LOCUS       DASTSP010000001         3963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1351095, whole genome shotgun sequence.
ACCESSION   DASTSP010000001 DASTSP010000000
VERSION     DASTSP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351095"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFS75_00005"
     CDS             <1..404
                     /locus_tag="VFS75_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4677087.1"
                     /translation="GAVFKSSTEEVLREADVVSLHVPLLPSTTHLINAERLSMMKPTA
                     YLINTSRGPVIDEAALVAALKAGTIRGAGLDVFENEPALAPGLAECENAVITPHIASA
                     SEETRNKMSEIAAENVIQFLKGQNPENAVTE"
     gene            470..1117
                     /locus_tag="VFS75_00010"
     CDS             470..1117
                     /locus_tag="VFS75_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU4677088.1"
                     /translation="MTFSLTVEERKERGKQLARLRSAGKLPAVMYGPKETATAITLDK
                     GKFEKLFKETGESSVIVLDGLGEPKEVLVHDVSFDPRKGGIIHVDFYAVEAGKEITVN
                     VPLEFVGEAPAVKQGGTLTKVLHEVEITAKPANLPQHLTVDVSSLDDFEKRITVRDLE
                     VPAGAKLENDLEDVVALVQAVEEEKEAPVEAVDMSAIEVEEKGKKEEPTEGAEAA"
     gene            1223..2584
                     /locus_tag="VFS75_00015"
     CDS             1223..2584
                     /locus_tag="VFS75_00015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEU4677089.1"
                     /translation="MRTYAVAFITTIVGIASLFPVAPVLAAGACDDDTTGKTEEELKA
                     DLAQCNAEIAQWQGILNGTRNKVTTIDSDVKALTAKIKAAEATIKSKNIAISQLSEDI
                     NERSEKIQDLEDKINEGKESLAQLIRRTNEIDDSTLAEVVLNSKNLSEFFSDLDSFFD
                     VQRGLEDHFRDVRQTIAQTEEERERLGEMQDKELDAKYVIETNKKTITKTQAEKQQLL
                     AATKQEAKSYEQIVKDRQAKANQINAALFRLRGVDGGGIPFKEALAYAKAASSYTGVR
                     AAFILAILRQESNLGVNVGQCLLVNPQTGAGKGKNTGTPFANVMKPDRDIPYFLDLMK
                     RLGRDPYATPVSCPQSVGYGGAMGPTQFIPSTWRSYEPRIAQAFGQSVADPWDPESAI
                     MATALYLEDMGAARGGYTAEREAAARYYAGGGWATYGLGYAASVLAYAEQYQNDIDFL
                     SSQ"
     gene            2626..2895
                     /locus_tag="VFS75_00020"
     CDS             2626..2895
                     /locus_tag="VFS75_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU4677090.1"
                     /translation="MKKDIHPENYRLVIFHDNSSGERFLVGSTIATEATDVWTDKKEY
                     PIAYVDVSSASHPFYTGQEKVMDTAGRVERFKARAEKAKSRAKKA"
     gene            2957..3928
                     /locus_tag="VFS75_00025"
     CDS             2957..3928
                     /locus_tag="VFS75_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU4677091.1"
                     /translation="MDYDQSKLAEFRENQRTQFLAGQFDELSRKHAEAAALLASDASM
                     AELAEDEMRSLEEQRAALFAEMERIVVESKEEEARPYGVILEVRAGVGGEEAALFAEE
                     LAHMYLKYGERKGWRTATASESRAAQGGYKEASFELLGSDVYDDLRYETGVHRVQRIP
                     VTEKAGRIHTSTASVAVLPMRRKPSIDVNPGDIEMEFSRSGGAGGQNVNKVETAVRLI
                     HKPTGVDVRCQSERSQLKNREKAMAMLIAKLEMLHEEEEAKRHSESRRSQIGTGDRSE
                     KIRTYNVPQNRITDHRIKESWHAIDDILGGELDEVIASLKRHAEMAG"
BASE COUNT          870 a         1170 c         1301 g          622 t
ORIGIN      
        1 ttggcgccgt cttcaagtct tcgaccgagg aggtgctccg cgaagccgac gtcgtctccc
       61 tccatgttcc gctcctcccg tcgacgacgc acctcataaa cgcggagcgt ctttcgatga
      121 tgaagccgac cgcgtacctc atcaatacgt cgcgcggacc cgtcattgac gaggcggcgc
      181 tcgtcgccgc cctcaaggcg ggaaccatcc gaggcgcggg actcgacgtt ttcgagaacg
      241 aaccggcgct cgcccctggc ctcgccgagt gcgagaatgc cgtcatcacc ccgcacatcg
      301 cctcggcgtc cgaggagacg cgcaataaga tgtccgagat agccgcggag aacgtcattc
      361 agttcctcaa ggggcagaat cccgagaacg ccgtcaccga gtagagcccg aagacttgag
      421 gtttttggca cgtatggtat ggtagtcggc actaatacta atggggcata tgacattttc
      481 acttaccgtt gaggagcgaa aggagcgcgg caagcagctt gcgcgtcttc ggagcgcggg
      541 gaagctcccg gccgtcatgt acggaccgaa ggagaccgcg acggcgatca cgctcgataa
      601 gggcaagttc gaaaaactct tcaaggagac cggcgagtcg agcgtcatcg tgctcgatgg
      661 gctcggagaa ccgaaggagg ttctcgtcca cgacgtctcg ttcgatccgc ggaagggagg
      721 catcatccac gtcgacttct acgccgtcga ggcgggcaag gagatcaccg tgaacgtgcc
      781 cctcgagttc gtcggcgagg cgccggccgt gaagcagggc ggaaccctca cgaaggtgct
      841 ccacgaggtc gagatcaccg cgaagccggc gaacctcccc cagcacctca ccgtcgacgt
      901 ttcctcgctc gacgatttcg aaaagcgcat caccgtgagg gatctcgagg ttcccgcggg
      961 cgcgaagctc gagaacgacc tcgaggacgt cgtcgccctc gttcaggccg tcgaggagga
     1021 gaaggaagcg ccggtcgagg ccgtcgacat gtccgcgatc gaggtcgagg agaagggcaa
     1081 gaaagaggaa cccacggaag gcgcagaggc ggcgtagatc taaggaaagc ggaaaaacgg
     1141 ccccgaacgg ggccgttttt cgaacccccg tgcacgagcg cctagaaatg acgcccggta
     1201 tcttggtata ctggagggac acatgaggac atacgcggtc gcattcatca cgacgattgt
     1261 tggtattgct tcgctctttc ccgtggcgcc ggttctcgct gcgggcgcgt gcgacgacga
     1321 tacgaccgga aagacggaag aggagttgaa ggccgatctc gcgcagtgta acgcggagat
     1381 cgcgcagtgg cagggaatcc tcaacggtac gcgcaacaag gtgacgacga tagacagcga
     1441 tgtgaaggcg ctcaccgcga agatcaaggc ggccgaggcg accattaagt cgaagaacat
     1501 cgcgatatcc cagctctccg aggacatcaa cgagaggagc gagaagatac aggacctcga
     1561 agacaagata aacgagggga aggagtcgct cgcccagctc attcgccgta cgaacgagat
     1621 agacgattcg acgctcgccg aagtggtgtt gaacagcaag aacctctcag agttcttctc
     1681 cgacctcgac tccttcttcg atgtccaacg cggactcgag gaccacttca gggacgtgcg
     1741 gcagaccatc gcacagaccg aggaggaacg agagcgtctc ggtgagatgc aggacaagga
     1801 gctcgacgcg aagtacgtca tcgagacgaa caagaagact atcacgaaga cgcaggcgga
     1861 gaagcagcaa cttctcgccg cgacgaagca ggaagcgaag tcgtacgagc agatcgtgaa
     1921 ggaccgccag gcgaaggcga atcagatcaa tgcggcgctc ttccggctcc gtggcgtcga
     1981 cggcggcgga attcccttca aagaggcgct cgcgtacgcg aaggccgcgt cgtcgtacac
     2041 cggcgtccgc gccgcgttca tcctcgccat cctccgccag gagtcgaacc ttggcgtgaa
     2101 cgtcggccag tgtctcctcg tcaacccaca gaccggcgcg gggaagggta agaacacggg
     2161 gacgcccttc gcgaacgtca tgaagccgga ccgcgacatc ccgtacttcc tcgacctcat
     2221 gaagcgcctc ggacgcgatc cgtacgcgac gccggtctca tgtccgcagt cggtgggcta
     2281 cggcggggcg atggggccga cccagttcat cccgtcgacg tggcgaagct acgagcctcg
     2341 tatcgcacaa gcgttcggcc aatcggtcgc ggacccttgg gacccagaga gcgccatcat
     2401 ggcgaccgcg ctctatctcg aggacatggg cgcggcccgg ggaggctaca ccgcggagcg
     2461 cgaggcggct gcccgttact acgcgggcgg cggatgggcg acgtacgggc ttgggtacgc
     2521 ggcgagcgtc ctcgcgtacg cggagcagta ccagaacgac atcgacttcc tctcgagcca
     2581 gtaaacggtt gccaaacgcg acatttctgc tatagtggtc cggcaatgaa aaaggacatt
     2641 cacccagaaa actatcgtct cgtcatcttc catgacaact cgagcggcga gcgttttctc
     2701 gttggctcga cgatcgcgac cgaggcgacc gacgtctgga ccgacaagaa ggagtacccg
     2761 atcgcgtacg tcgacgtgtc gtcggcatcg cacccgttct atacgggcca ggagaaggtg
     2821 atggacaccg ccggacgcgt cgagcgcttc aaggctcgcg cagagaaggc gaagagccgc
     2881 gcgaagaagg cgtaagcggg aaggtgagac cggagacagc tccggtttta tcgtgtctcg
     2941 tactatacta acgttcatgg attacgatca gtcgaaactc gccgagttca gggagaacca
     3001 gaggacacag ttcctcgcgg gtcagttcga cgagctctcg aggaagcatg cggaggccgc
     3061 tgcgcttctt gcgagcgatg cctcgatggc ggagctcgcg gaagacgaga tgcgctcgct
     3121 cgaggaacag cgcgcggcgc tcttcgcgga gatggagcgt atcgtcgtcg agtctaagga
     3181 agaggaggcg cggccgtacg gcgtcatcct cgaagtccgt gcgggcgtgg gcggcgagga
     3241 ggcggcgctc ttcgcagagg agctcgcgca catgtacctc aagtacggag agcggaaggg
     3301 ctggcggacg gcgaccgcgt ccgaatcgcg cgccgcgcag ggcggctaca aggaggcttc
     3361 gttcgagctt ctcggttcgg acgtgtacga cgacctccga tacgagacgg gcgtgcaccg
     3421 ggttcagcgc attccggtaa ccgagaaggc ggggcgcatc cacacgtcga ccgcgtcggt
     3481 cgccgtactc ccgatgcgtc ggaagccctc gatcgacgtc aatcccggcg acatcgagat
     3541 ggagttctcc cgttcgggcg gtgccggcgg acagaacgtg aacaaggtgg agacggccgt
     3601 ccgccttatc cataagccga ccggtgtcga cgtgcgctgt cagtcggaac ggagccagtt
     3661 gaagaatcgc gagaaggcga tggcgatgct catcgcgaag ctcgaaatgc tccacgagga
     3721 ggaggaggcg aagcggcact ccgagagcag gcggagccag atcggcaccg gcgaccgctc
     3781 cgagaagata cggacgtaca acgtgccgca gaatcgcatc accgatcacc gcatcaagga
     3841 atcgtggcac gcgatcgatg acatcctcgg cggggagctc gatgaggtga tcgcatccct
     3901 caagcgccac gcggaaatgg cgggatgacg ccgcctgttg cggaaacggg ggcgctgtgg
     3961 tac
//
LOCUS       DASTSP010000002         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1576055, whole genome shotgun sequence.
ACCESSION   DASTSP010000002 DASTSP010000000
VERSION     DASTSP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576055"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFS75_00030"
     CDS             <1..529
                     /locus_tag="VFS75_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677092.1"
                     /translation="LSSYLIIDTILKTFVTGADLGPWNEVKCESAPPVTPTGTTPPVT
                     PAAGTYTDADARAALAAAGITVNKTEAQGTSLNGMRTKTVQDVIDLKHDCDCTIVITG
                     GTESTGGHAEGTYSHESGYKYDMRVNDTLDGYIYSSFDKIKTRSDGAEQYRNPKDGTI
                     YAREGDHWDVLVPPG"
     gene            569..639
                     /locus_tag="VFS75_00035"
     tRNA            569..639
                     /locus_tag="VFS75_00035"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Trp,seq:cca)
     gene            921..2123
                     /locus_tag="VFS75_00040"
     CDS             921..2123
                     /locus_tag="VFS75_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU4677093.1"
                     /translation="MRAATNSVFHTSRKEHFMQKSDMTSRGDKPDWYLGSQAPSFGDY
                     FGSVSSLAVGETYLTYDRKYIVHRVAATEFHVSRNACAHAGAPILETPGVQDVDNLRC
                     PIHKWTYDLKGQLKAAPQFKSCHGVSLSKPDVALWNGNLVGFRKEETESLSRFGASLS
                     LPRGAFDTSAFVFMGEERYPIPYPRELMLVNYFDGYHVPLYHQKTFAAVANCDSYAWE
                     FSEQGAKVGYSIQVVRARSDVEKERRFLMQRGLAEEDIGWATFHLWIRDEFKSVERPI
                     DADIFAVWASLYGNGFLMPELYEGGLFLAMSYLVNEDPGNAETGNANLVEYYVHKNVP
                     EARRKEAARRFQHAYEQSAREDDEICETLYRSHRHRNFSDNRIYHQFLEAGDVHWREW
                     YKLKSRFK"
     gene            complement(2160..3047)
                     /gene="miaA"
                     /locus_tag="VFS75_00045"
     CDS             complement(2160..3047)
                     /gene="miaA"
                     /locus_tag="VFS75_00045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU4677094.1"
                     /translation="MKPKIIVIVGPTASGKTALSVELALRLRGEVVSADSRQVYRGCD
                     LGTGKVTREEMRGVPHHLLDVADPREGYTVADFVRDGRTAIDDIRSRGNVPIVVGGTF
                     FYIDALLGRVSAPDVPPNEELRARLEPLPAPDLYELLRMRDPARAAAVDPANKRRLIR
                     ALEIVEAKGIVPPHAPDEVYDALTIGIRVAPAVLRESIRARLEERIKAGMIDEVVDLE
                     ESGIPFERIESLGIEYEHIVAFLKGSCDEVELRERITTKSYQYAKRQMTWLKRDATIR
                     WVEPGDIQTTLTLVKDFLS"
BASE COUNT          679 a          963 c          992 g          645 t
ORIGIN      
        1 cctctcctca tacctcatca tcgacacgat attgaagacg ttcgtcaccg gtgccgacct
       61 cggtccgtgg aacgaggtga agtgcgaatc agcgccaccg gtcacgccga cgggaacgac
      121 cccgccggtg acaccggctg ctggaacgta taccgacgcg gatgctcgtg ccgcgctcgc
      181 ggccgccggt atcaccgtca ataagacgga agcacaagga acctcgctca acggcatgcg
      241 gacgaaaacc gttcaggatg tcatcgacct aaagcacgac tgtgactgta ctattgtcat
      301 aaccgggggc accgagagca cgggcggaca cgccgaaggt acctacagcc acgagagcgg
      361 gtataagtac gatatgcgcg tgaacgacac gctcgatggc tacatttaca gcagttttga
      421 caagatcaag acgcggagcg atggcgcgga acagtatcgt aatccgaagg acgggacaat
      481 ttacgcgcga gaaggggacc attgggacgt tctcgttcca cccggttgac ccgttgtatc
      541 ctttctcgta ggatggtttg gcggaaacgg gcctatagtt cagtggtaga atgctggtct
      601 ccaaaaccag cgaccgaggt tcgaatcctc gtgggcccgc aaaggtatac gaaaataacc
      661 tgctaaaaat gcaggttatt ttcgtatagc gagcaaactg ctttgctcgc ggacctttgc
      721 gggagacgga gctgtgtttt gcgctatacg aggcgcaaaa caagcgagtc cggggaggaa
      781 gttcttagga gcgtagcgac ttagaaactt gaccccgcaa aaccgataac aatcgaatcc
      841 catcacgcgt tagtgactcg tgaccgcaaa cgttgcacgt aaaccccgtc cgcttgccct
      901 catccaccca ttggggtacc atgcgcgcgg ctacgaattc ggtctttcac acttcacgaa
      961 aggagcactt catgcaaaag agcgacatga cgtcgcgcgg cgacaagccg gattggtacc
     1021 tcgggagtca ggcgccttcc tttggcgatt acttcggctc ggtgtcgtcc ctcgccgtcg
     1081 gggagacgta cctcacgtac gatagaaagt acatcgtgca ccgcgtcgcc gccacggagt
     1141 ttcacgtctc gcgaaacgcg tgcgcccacg cgggtgcgcc gatcctcgag acgccgggcg
     1201 tccaggacgt cgacaacctc cgctgtccga tacataagtg gacgtacgac ctcaagggac
     1261 agctcaaggc ggcgccgcag ttcaagtcct gccatggtgt gtcgctctcg aaacctgacg
     1321 tcgcgctctg gaacggaaac ctcgtcggtt ttcggaagga ggagaccgaa tccctcagca
     1381 gattcggcgc gtcgctcagt cttccaaggg gtgcgttcga cacctcggca ttcgtcttca
     1441 tgggcgagga gcgttatccg atcccgtacc ctcgtgagct catgctcgtc aattacttcg
     1501 acgggtacca cgttcccctg taccaccaga agaccttcgc ggcggtcgcg aactgcgact
     1561 cgtacgcgtg ggagttctca gagcagggcg cgaaggtggg gtacagcatc caggtggtgc
     1621 gcgcacggag cgacgtcgag aaggaacgga gattcctcat gcagcgcggt ctcgccgagg
     1681 aggatatcgg ctgggcaacg ttccacttat ggatccggga cgagttcaag tcggtcgagc
     1741 gcccgatcga tgccgatatc ttcgcggtgt gggcgtcgct ctacgggaac gggtttctca
     1801 tgccggaatt gtacgaaggc ggtctcttcc tcgcgatgag ctacctcgtc aatgaggacc
     1861 cgggcaacgc cgagacgggg aacgcgaatc tcgtcgagta ctacgtgcac aagaacgtac
     1921 ccgaggcgcg ccggaaggaa gcggcgcgcc ggttccagca cgcgtatgag cagagcgcgc
     1981 gcgaggacga cgagatctgc gagacgctct atcggtcgca tcgtcaccgg aacttctcgg
     2041 acaatcgcat ctaccaccaa ttcctcgagg cgggtgacgt gcactggagg gaatggtaca
     2101 agctcaaatc acgcttcaag tgaagtatcg tatcgcggcc cgtttcgacg ggccgttttt
     2161 tatgacaaaa aatcctttac gagagtcagg gtcgtctgta tatcgcccgg ctctacccag
     2221 cgaatggtcg catcgcgctt gagccaggtc atctggcgct tcgcgtactg gtagcttttt
     2281 gtcgtgatgc gctcacgcag ttcaacctcg tcgcaggaac ctttgagaaa tgcgacgatg
     2341 tgttcgtatt cgattccgag tgactcgata cgctcgaagg ggatgccgct ctcttcgaga
     2401 tcgacgacct cgtcgatcat acccgccttg atgcgctcct cgagacgcgc tcggatgctc
     2461 tcgcggagaa cggccggagc gacgcgaatg ccgatggtga gcgcgtcata cacttcgtct
     2521 ggcgcatggg gtggcacgat acccttcgcc tcgactatct cgagtgcgcg gatgagccgg
     2581 cgcttgttcg cggggtcgac ggccgcggcg cgcgcggggt cacgcatgcg gaggagctca
     2641 tagaggtccg gtgcgggaag cggctcgagc cgtgccctaa gttcctcgtt cggcggcacg
     2701 tcaggtgcgc tcacgcgacc gaggagcgca tcaatataga aaaatgtacc gccgacgacg
     2761 atgggaacgt tgccccgcga gcgaatatcg tcgatggcgg tccgtccatc tcggacgaaa
     2821 tccgcgaccg tgtaaccttc tctcgggtcg gccacatcga ggaggtggtg cggcacgccg
     2881 cgcatctcct cccgcgtcac cttgccggtc ccgaggtcgc acccgcggta tacttgccgc
     2941 gagtcggcgg agacgacttc gcctcgaagt cgaagcgcga gctcgaccga gagcgcggtc
     3001 tttcccgagg cggtcggtcc gacgatgacg atgattttcg gtttcatacg aggagggcca
     3061 ggaacgcgtc ctggcccgat cttcaatttc ttcggaggaa attccttaag cgctcaatga
     3121 acgaaggctg acgcgggtcg aggaaggagt ggccctccag ggagcccttc cattcctcga
     3181 cgtccttctt atcgatgagt ccgagcctcc tattcacgaa ctttcgctac gcctcgggat
     3241 cgttcggatg ctccttacgc gcgtccgcct ccgcgttag
//
LOCUS       DASTSP010000003         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_237556, whole genome shotgun sequence.
ACCESSION   DASTSP010000003 DASTSP010000000
VERSION     DASTSP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237556"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="ruvB"
                     /locus_tag="VFS75_00050"
     CDS             complement(<1..722)
                     /gene="ruvB"
                     /locus_tag="VFS75_00050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU4677095.1"
                     /translation="MSDQATTRPSFERDSAGNLDQTLRPQTWEEYVGQPKTKENLRIL
                     LTAARERGHAAEHILLYGPPGLGKTTLAHLISKTLGTNMRTTSGPAIERVGDLAAILT
                     NLSPGDVLFIDEIHRLSKSIEEVLYPAMESGVLDIIIGKGPSARTVQLDLPPFTLVAA
                     TTRISMLSAPLRSRFSGGTFRLEFYSEDEIAEILRRSASLLNVRADDALLASIAKRCR
                     ATPRTANYLLKRCRDLAQLEGR"
     gene            complement(1063..2715)
                     /locus_tag="VFS75_00055"
     CDS             complement(1063..2715)
                     /locus_tag="VFS75_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU4677096.1"
                     /translation="MAEFNDSNTNARIDELRHREEERLVQQLAPSYGYPYVNLGDVPI
                     DVSALKLLSEADARRGELAVFAQAGQNVSVAVRNPKNPELQALLEKLASDGYTPTVYL
                     ASFPSIDRAWARYHDIGRSTVQVKGVLDVDPELIKAFANEIKSSLDVAGKVAEIQKEN
                     SVVKTSKTIGAIFGGALSLGASDVHIEPEALAVRVRYRLDGVLTNVCDIDKDIAAHIV
                     SRLKLLSGLKLNVRREAQDGRFTFDIGTREVEVRSSVIPGAYGESMVMRLLDPNASSF
                     KLENLGLSEKLHSIMEEELKRPNGAILTTGPTGSGKTTALYSFLLSIHTPEIKIITIE
                     DPVEYKLPGIVQTQVSETYSFEEGLRSVLRQDPDVILIGEIRDREVAETAIHAALTGH
                     LVFSTLHTNSAAGAFPRLIDLGIDANMIGSGVNIILGQRLVRLLCDACKVERPITTEE
                     QKLIQGVLGQSIAIHTIFDAKGCDRCGGSGYKGRVGVYEAIKMDEKVREVVATDTREA
                     AIREAAKHQGIPSMQEDGIMKVLGGLTSLDEVARVLDLYNAG"
     gene            2851..3372
                     /locus_tag="VFS75_00060"
     CDS             2851..3372
                     /locus_tag="VFS75_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677097.1"
                     /translation="MKVLWNVAVFFGIAIALSACGGVTVRHTYGKAEALGSRASCPAP
                     FDKDAIAKVGYPEVWAREPHEPATVWPLYRCSFMQPLIAWAKSASNYESDRGGCTLDY
                     QSFTCRSHLGPSGTSRHGRVTYLEGSSRIDVAIANLSVLSCPNAGAEDRELDVSVDPD
                     GRWRFSVRHECYY"
BASE COUNT          617 a         1033 c         1175 g          718 t
ORIGIN      
        1 tcgcgtccct cgagctgggc gaggtcgcga cagcgcttca agaggtagtt cgcggttcga
       61 ggcgtcgcgc ggcagcgctt cgcgatggat gcgaggagcg cgtcgtccgc gcggacattg
      121 agtagcgacg ccgagcggcg aaggatctcc gcgatctcgt cctcggagta gaactcgaga
      181 cggaacgtgc cgccggagaa gcgcgaacga agcggcgcgg aaagcatcga gatgcgggtc
      241 gtcgcggcga cgagcgtaaa gggcgggagg tcgagctgga cagtgcgcgc cgacggtccc
      301 ttgccgatga tgatatcgag aacccccgac tccatcgccg ggtagagtac ttcctcgatc
      361 gatttcgaga ggcggtggat ctcgtcgatg aagagaacgt cgccaggcga gaggttcgtg
      421 aggatggcgg caaggtcacc gacgcgttcg atcgcggggc cggacgtcgt ccgcatgttc
      481 gtgcccaagg tcttcgaaat gaggtgcgcg agcgtcgtct tcccgagccc cggcggaccg
      541 tagaggagga tatgctcggc ggcatggccg cgttcgcggg cggcggtgag gaggatgcgg
      601 aggttctcct tggtcttcgg ctgaccgacg tactcttccc acgtctgcgg gcggagggtc
      661 tggtcgagat tgcccgcgct atcgcgctcg aaggaaggcc gcgtcgtcgc ttggtcgctc
      721 atgggcccta ttgtatacga aaaaccgggg cggggtgagg gggttgacaa acctgactac
      781 catgctaaaa tagcctgtaa tcgttcgttg cattcgagga cactcgaatg cttttctttt
      841 gcatcgggca aaaaagggcg tattcgcgcg gcgttctgtc gagtcgatac ccctctaagc
      901 aatacatggc tctcaaggcg agggtactct ggtacccgct tcgagcgcga tcactgggct
      961 attccttagc tttggagggt cacttcttcc gggaagctat tgcttaaagg gttatccgtt
     1021 cgacggccga aaaaagggga ccttcgggtc ccctttcgcg tgtcagcccg cgttgtagag
     1081 gtcgaggacg cgcgcgacct cgtcgagcga cgtgaggcca ccgagaacct tcatgatgcc
     1141 gtcctcctgc atcgagggaa tgccctgatg cttcgccgcc tcgcggatcg cggcttctcg
     1201 tgtgtccgtc gcgacgacct cgcggacctt ctcgtccatc ttgatcgcct cgtagacgcc
     1261 gactcggcct ttgtagccgc tcccgccgca gcggtcgcat cccttcgcat cgaagatcgt
     1321 gtgaatggcg atcgattgtc cgagcacgcc ctggatgagc ttctgctcct ccgtcgtgat
     1381 cggacgctcg accttgcacg cgtcgcagag gagacgaacg aggcgttggc cgaggatgat
     1441 gttgacgccg cttccgatca tgttcgcgtc gatgccgagg tcgatgagcc gcgggaaggc
     1501 gcccgcggcg ctattcgtgt gcaacgtcga gaagacgagg tgaccggtga gcgcggcatg
     1561 tatcgccgtc tccgcgacct cgcggtcgcg gatctcgccg atgagaatga cgtccgggtc
     1621 ttggcgaaga acggaacgga gcccttcctc gaacgagtac gtttcgctca cctgcgtctg
     1681 cacgatgccg gggagcttgt attcgaccgg gtcttcgatc gtgatgatct ttatctcggg
     1741 cgtatggatg gagagaagga acgagtagag cgcggtcgtc tttcccgagc cggtcggtcc
     1801 ggtcgtgagg atcgcgccgt tcggtcgctt cagttcctcc tccatgatgc tatggagctt
     1861 ctccgagaga ccgaggtttt cgagcttgaa ggacgaggcg ttcgggtcga ggaggcgcat
     1921 caccatcgat tcgccgtacg cgccgggaat gaccgacgag cggacttcga cttcgcgcgt
     1981 tccaatgtca aaggtgaagc ggccgtcttg cgcctccctt cgtacgttca atttgaggcc
     2041 cgagaggagc ttgaggcgcg agacgatgtg cgcggcgatg tccttatcga tgtcgcagac
     2101 gttcgtgaga acgccgtcga gccggtagcg gacgcggacg gcgagcgcct ccggctcgat
     2161 gtggacgtcc gaggcgccga gggaaagtgc gccgccgaag atggcaccga tcgtcttgct
     2221 cgtcttcacg acgctgttct ccttttggat ctccgcgacc ttgcccgcga cgtcgagact
     2281 cgacttgatc tcgttcgcga acgccttgat gagctcgggg tcgacgtcga gaacgccctt
     2341 cacctgaacg gtcgagcggc cgatgtcgtg gtagcgtgcc cacgcgcggt cgatcgacgg
     2401 gaacgaggcg aggtacacgg tcggcgtata gccgtcactc gcgagcttct caaggagcgc
     2461 ctgaagctcc gggttcttcg gattgcgtac cgctacggag acgttctgtc cggcctgtgc
     2521 gaagacggcg agctcgccgc gacgcgcgtc tgcctcggag aggagtttga gcgccgaaac
     2581 gtcgatgggg acgtcgccga ggttcacgta cgggtagccg tacgagggcg cgagctgttg
     2641 cacgaggcgt tcctcctccc tatgccggag ctcgtcgatg cgggcgttcg tgttcgagtc
     2701 attgaactct gccatttctc tcttatggta ccacgggcaa gggagcggcg agtcggattt
     2761 tcaaccttat tttaagccag aaacggatat tgacgcatac aatattcgtt gtataattgt
     2821 actatactcc ataccccaaa ggaggggata atgaaggttc tttggaacgt cgcggtcttc
     2881 ttcggcatcg ccatcgccct ctccgcgtgc ggcggcgtca cggtgcggca tacatacggg
     2941 aaggcggaag cgctcggctc gcgcgcgagc tgtccggcgc ccttcgacaa ggacgccatc
     3001 gcgaaggtcg ggtacccgga ggtgtgggcg cgggagcccc acgaaccggc gaccgtgtgg
     3061 cccttgtacc gttgctcgtt catgcagccc ctcatcgcgt gggcgaagtc ggcgagcaac
     3121 tacgagagcg accggggcgg ttgcacgctc gactaccagt ccttcacgtg caggagccac
     3181 ctcggtccct cgggaacgag ccgccatggg cgcgtcacgt atctcgaggg ttcgagtcgt
     3241 atcgatgtcg ccatcgcgaa cctctcggtc ctcagttgtc cgaacgcggg cgcagaggac
     3301 cgcgagctcg atgtctccgt cgaccccgac gggcggtggc gcttctcggt tcggcacgag
     3361 tgctactact aacggcggcc tgtccgcgag aggccggttt tcgatccttt tggatcgaaa
     3421 accggccttt ttctttacat gctagaatgc tcggatggaa cgactgacgg cggctaatct
     3481 cgaggaactc caaacggccg cgcgccgcgt cctcgcggcg cttcaagcaa gtgaccatgc
     3541 gac
//
LOCUS       DASTSP010000004         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_910825, whole genome shotgun sequence.
ACCESSION   DASTSP010000004 DASTSP010000000
VERSION     DASTSP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910825"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFS75_00065"
     CDS             complement(<1..143)
                     /locus_tag="VFS75_00065"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEU4677098.1"
                     /translation="MANDTSPIDIQKYLKGVNYPCSKQELVEAAKREGAPENVLGVLN
                     AMK"
     gene            254..1297
                     /locus_tag="VFS75_00070"
     CDS             254..1297
                     /locus_tag="VFS75_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU4677099.1"
                     /translation="MDLVSTWTLTTTSEAAWEEMIASMSRAKRTIDVLQFAVGATGPF
                     VDVFVDVLTKKAGEGVRVRILADAIGSFLFYQSPARKRLEEAGVDVLFHAAVVPRSLR
                     RLVPAFLRDHRKLVVVDDAEAHIGGVILEERARYWRDTSVVVRGAIVEDASALFETSW
                     SHAKRMKPLGTLLSEDGRGPFYLAGNSYRLGDKTLYRAMVRALTKAKRRVSITTPYFA
                     LTRDLRRALLYAMDQGAEVRILLPRRSDNVWADMLGRLFYAWLLRRGVRIFHYSRAIL
                     HAKTMVIDGTWATIGSSNLDWLSIWLNYELNLVSDVPRFAAELERLFEHDLETCTEVT
                     RSTKHWYGFFGDD"
     gene            1356..1523
                     /locus_tag="VFS75_00075"
     CDS             1356..1523
                     /locus_tag="VFS75_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEU4677100.1"
                     /translation="MLYWAAVFFILALVAAVLGFGNVVVVAWVGVAKLLFYIFLIAFV
                     VTLLIGVFRGP"
     gene            1678..2148
                     /locus_tag="VFS75_00080"
     CDS             1678..2148
                     /locus_tag="VFS75_00080"
                     /inference="COORDINATES: protein motif:HMM:NF021390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEU4677101.1"
                     /translation="MRKYLDGRVLALVAIALAIGYAAVLRQPKPEAPAVIGGDRDEHG
                     CLIAAGYSWCDARGECERPWERYCTTAAPKLAHFSCDAGKTIDATFYPADDLFVDLVL
                     GDERNMSIPRAISASGARYAREDESFVFWNKGDTAFITENGTTTYENCGIAGAR"
     gene            complement(2145..>2378)
                     /locus_tag="VFS75_00085"
     CDS             complement(2145..>2378)
                     /locus_tag="VFS75_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677102.1"
                     /translation="VIVLISQNREARIAELREEVELYVNTYSEAEITKVMHLLILLLE
                     KNGIDISKETDVQDMLTSLEADKIEKELERQLH"
BASE COUNT          413 a          731 c          723 g          511 t
ORIGIN      
        1 tccttcatcg cgttcaagac gccgaggacg ttctccggcg cgccctcgcg cttcgccgcc
       61 tcgacgagct cttgcttcga gcacggatag ttcactccct tcaggtactt ctggatgtcg
      121 atgggactcg tatcgtttgc catggtagga aattatcgag taagtacaca cctgtaccat
      181 agcaacaact cgattaagta cgaatgaagc gattttcatg acggcatcat cgccggctcg
      241 ctaccgtact cgtatggacc ttgtctccac gtggactctc acgacgacct ccgaggcggc
      301 gtgggaggag atgatagcgt cgatgagccg cgcgaagcgc acgatcgacg tcctccagtt
      361 cgcggtcggc gcgacgggcc ctttcgtcga cgtgttcgtc gacgtcctta cgaagaaggc
      421 gggggagggt gtacgcgttc gcattctcgc ggacgcgatc ggcagtttcc tcttctacca
      481 gtcacccgcg agaaagcgcc ttgaggaagc gggtgtcgac gttctcttcc acgcagcggt
      541 cgtaccgcgt tcgctccggc gcctcgttcc cgcgtttctc cgcgaccacc gaaagctcgt
      601 cgtcgtggac gacgccgagg cgcacatagg gggcgtcatt ctcgaggaac gcgcgcggta
      661 ctggcgtgat acgagcgtcg tcgttcgggg cgcgatcgtc gaggacgcgt cggcgctctt
      721 cgagacgtcg tggtcccacg cgaagcgcat gaagccgctc ggcacgctcc tctccgagga
      781 tgggagaggg ccgttctacc tcgccggaaa ctcgtatcgc ctcggcgata agacgctcta
      841 tcgcgcgatg gtccgcgcgc tcacgaaggc gaagcggcga gtgtccatca cgacaccgta
      901 cttcgcgctc acgcgcgacc ttcggcgcgc gcttctctat gcgatggacc aaggggcgga
      961 ggtccgtatt cttctcccgc ggcgctccga caacgtttgg gccgatatgc tcggccgcct
     1021 cttctatgcg tggctcctcc ggcgcggcgt ccgcatcttt cactattcgc gcgccattct
     1081 ccacgcgaag acgatggtca tcgacggcac atgggcgacg atcggaagtt cgaacctcga
     1141 ctggctctcc atttggctca attacgaact gaacctcgtg tccgacgtgc cacgcttcgc
     1201 cgcggagctc gagcggctct tcgagcacga cctcgagaca tgcaccgagg tgacgcgctc
     1261 gacgaagcac tggtacggct tcttcggcga tgattgacag cacgtgtcga tgggcgtaga
     1321 ttcttcggag tcttccacca gcgaaaggag agtctgtgct ctattgggcc gccgtgttct
     1381 tcatcctcgc cctcgtcgcc gccgttctcg gcttcggaaa cgtcgtggtc gtcgcctggg
     1441 tcggcgttgc gaagctcctg ttctacatct tcctcatcgc gttcgtcgtg acgctcctca
     1501 tcggagtgtt tcggggcccg tgatcagcac gcggcgacct tcgggtcgcc gttttcagtg
     1561 cgacgttttt gacgtcgcgg tatcgaggaa ccgcgccgcc atagcgcgat ggaatcgatg
     1621 gtatcggaga agattgagac gagtggtgaa tccgaacggg cggcgtatac ttactacatg
     1681 agaaagtacc tagacggacg ggtcctcgcc ctcgtcgcta tcgcgctcgc gatcggatat
     1741 gcggcggtcc ttcgtcagcc gaagcccgaa gcgccggccg tgatcggtgg cgaccgagac
     1801 gagcacggtt gtctcatcgc cgcaggatac tcatggtgcg atgcgcgtgg cgagtgcgag
     1861 cggccatggg agcgctactg cacgacggcc gcgccgaaac tcgcgcattt ctcgtgcgac
     1921 gcggggaaga ccatcgacgc cacgttctat ccggccgacg acctgttcgt cgaccttgtc
     1981 ctcggcgacg aacggaacat gtccatcccc cgcgccatct ccgcgtcggg cgcgcgctac
     2041 gcgcgcgagg acgaatcgtt cgtgttctgg aacaagggag atacggcgtt catcaccgag
     2101 aacggcacga cgacgtacga gaactgcggt atcgcaggcg cacgctagtg gagttgcctc
     2161 tcaagctcct tttcgatctt gtcggcttcg aggctggtaa gcatgtcttg gacgtccgtc
     2221 tctttcgata tgtctatgcc gttcttctcc aagagtaaaa tgagcaggtg catgaccttc
     2281 gtgatctctg cctcggaata cgtgttcaca tagagttcta cttcttcgcg aagctcggcg
     2341 attcgagcct cacggttttg cgagatgagg acgatgac
//
LOCUS       DASTSP010000005         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_689517, whole genome shotgun sequence.
ACCESSION   DASTSP010000005 DASTSP010000000
VERSION     DASTSP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689517"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFS75_00090"
     CDS             <1..590
                     /locus_tag="VFS75_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEU4677103.1"
                     /translation="IIIGKGPSARTVQLDLPPFTLVAATTRISMLSAPLRSRFSGGTF
                     RLEFYSEDEIAEILRRSASLLNVRAGDALLASIAKRCRATPRTANYLLKRCRDLAQLE
                     GRDLDETIIEKTFELLEIDELGLTGPDRALLSAIIEKFNGGPVGLNTMAAATGEEMST
                     IEDVFEPYLIRQGLLERTPRGRVASEAAYAHLARS"
     gene            745..1293
                     /locus_tag="VFS75_00095"
     CDS             745..1293
                     /locus_tag="VFS75_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007473900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4677104.1"
                     /translation="MSGHNKWSKIKRKKEVADAEKSKVFSKYASLIAVESRKAKGNLA
                     SPGLAAVIERAKKDHMPKENIDRAVQKGAGAEGSSLSEVLYETYGPGGVAILITAVTD
                     NPNRTTPEIRHTLSKQGYALGAAGSALWAFQKTGDGYVPTAPIALSDADGEKLANLVE
                     ALEENDDVQDIYTTADEAELPE"
     gene            1307..1780
                     /locus_tag="VFS75_00100"
     CDS             1307..1780
                     /locus_tag="VFS75_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU4677105.1"
                     /translation="MKVLAIDPGYDRLGVAVLTGTGSAPVLVHSSCILTERNTTLPDR
                     LHAAGAEVAALLEAHAPDVLAIETLYFNANATTAIAVAEARGIAIYLARTAGCEVREF
                     GPQEVKVAVTGYGKSDKRAVIDMVKRLCAGAPERGHDDEYDAIAVGITALAHHRA"
     gene            1873..2133
                     /locus_tag="VFS75_00105"
     CDS             1873..2133
                     /locus_tag="VFS75_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677106.1"
                     /translation="MLFSAHNDMREDEEDDEEAHIDDALLGELDDESLIDDELDPLIK
                     PELDPLALPAEDEEEETNKIFEDEEEDEEDMDYDSFDDRDEV"
     gene            complement(2182..3363)
                     /gene="tyrS"
                     /locus_tag="VFS75_00110"
     CDS             complement(2182..3363)
                     /gene="tyrS"
                     /locus_tag="VFS75_00110"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU4677107.1"
                     /translation="MKPSTDAMKIDDLLTRGVAEIIHEESLRARLTSGERLRVKLGID
                     PTSPHIHLGRAITLLKLRDFQELGHQVVFIIGDFTGQIGDTSDKESERPMLTRDVIEE
                     NLTTYLEQAGKILDMDAVECRRNSEWLEKLTYREIGEHADLFSVADFIARENIKRRLD
                     AGKRVSLREVLYPLMQGYDSVVVRADVEIGGTDQRFNLLAGRTLQEHAGERPQDVVMN
                     PLLNGTDGRKMSSSWGNTIALTADAADMYGKVMSIADSELLSYFMMCTRLPMSEVDAV
                     KDALASGTNPRDLKMRLAREITRMYHGDAESEAAEAAFVDTFQKGNVPEDVPEVGGDF
                     AAELIKAGIVASKTELRRLIEAGGVRNAETGEKLSAIPESVSEPVTLKVGKRRFVRLV
                     P"
     gene            3448..>4271
                     /locus_tag="VFS75_00115"
     CDS             3448..>4271
                     /locus_tag="VFS75_00115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU4677108.1"
                     /translation="MTRFTTWARKHTKELFIDLTIIFLVLGLIGAGVALVWISTLDIP
                     DLSSFEQRRVQQSTKIYDRTGEVLLYDLHQDVRRTVVPYERISRHIKNATVAIEDDTF
                     FEHKGVRPLAILRAAIANLEGGDLLGGQGGSTITQQVIKNAVLEPNKTLSRKIKEIIL
                     SIRLEQIMSKEDILGTYLNEVPYGGTIYGVEEASEAFFGKHASDVTLSEAAYLASLPQ
                     LPTYYSPYGSHFDALERRHDLVLDKMLEHGFITEDEYNQAKAEKVAFRPQEATGIKA"
BASE COUNT          844 a         1376 c         1286 g          765 t
ORIGIN      
        1 atatcatcat cggcaaggga ccttcggcgc gcacggtcca gctcgacctc ccgccgttta
       61 cgctcgtcgc cgcgacgacc cgcatctcga tgctttccgc gccgcttcgt tcgcgcttct
      121 cgggcggcac gttccgtctc gagttctact ccgaggacga gatcgcggag atactccgcc
      181 gctcggcgtc gctcctcaat gtccgcgcgg gcgacgcgct cctcgcatcc atcgcgaagc
      241 gctgccgcgc gacgcctcga accgcgaact acctcttgaa gcgctgtcgc gacctcgccc
      301 agctcgaggg acgcgacctc gacgagacga tcatcgaaaa gacgttcgag cttctcgaga
      361 tcgacgagct cggcctcacg ggaccggacc gcgcgctcct ctccgccatc atcgagaagt
      421 tcaacggcgg gccggtcggc ctcaatacca tggcggccgc gaccggtgag gagatgtcga
      481 cgatcgagga cgtcttcgaa ccgtacctca ttcgccaagg gctcctcgag cgcacgccgc
      541 gcggacgcgt cgcgtcggag gccgcgtacg cgcacctcgc acgctcgtag actttgcatt
      601 attcgccgga gcgattatca tgcagcaatt atcattgggt gctcgttgtg ggagagatct
      661 cgttcgtcgt tttgaggcgc cgtggggcgc acaaaacgac ggccaagatt ctctaattca
      721 ctacgttcat aacagaatct aggcatgtcc ggacataaca agtggtcgaa gataaagcgc
      781 aagaaggagg tcgcggacgc agagaagagc aaggtgttct cgaagtacgc gagcctcatc
      841 gcggtcgagt cgcgtaaggc gaagggcaac ctcgcgtcac ccggcctcgc cgcggtcatc
      901 gagcgcgcga agaaggacca catgccgaag gagaacatcg accgcgcggt acagaagggc
      961 gccggcgcgg aaggctcatc gctctccgag gtcctctacg agacgtacgg cccgggcggc
     1021 gtcgccatcc tcatcaccgc ggtgaccgac aacccgaacc ggacgactcc ggagatacgc
     1081 cacacgctct cgaagcaggg atacgcgctc ggcgcggctg gttccgcgct ctgggcgttt
     1141 cagaagaccg gtgacggcta cgtccccacg gcgcccatcg cgctctcgga cgccgacggc
     1201 gagaagctcg cgaacctcgt ggaggccctc gaggagaacg acgacgtgca ggacatctac
     1261 acgaccgccg acgaggccga gctccccgag tgataaaccg atttccatga aagttctcgc
     1321 catagacccg ggctacgacc gtctcggcgt cgccgtcctt acgggtacgg ggagcgcgcc
     1381 cgttctcgtc cattcctcgt gtatcctcac cgagcggaat acgaccctcc ccgaccggct
     1441 ccatgcggcg ggcgccgagg tcgccgcgtt gctcgaagcc cacgcgcccg acgtgctcgc
     1501 gatcgaaacg ctctacttca acgcgaacgc gacgaccgct atcgccgtcg ctgaggcgcg
     1561 cggcatcgcg atatacctcg cgcgcaccgc gggctgcgag gtccgcgagt tcggacccca
     1621 ggaggtgaag gtcgccgtga ccgggtacgg gaaaagcgac aagcgtgcgg tcatcgacat
     1681 ggtgaagagg ctctgcgcgg gcgcaccgga acgcggccac gacgacgaat atgacgcgat
     1741 cgccgtcggc atcacggcgc tcgcgcacca tcgcgcctaa gccctatcgt acccgaccgc
     1801 atctattgcg gaataaacga gagtctgata caaaggtttc gaactactaa gtaaccaaga
     1861 cacgcaacga acatgctgtt ttccgcacac aacgatatga gagaggacga ggaggacgac
     1921 gaggaggcgc acatcgacga cgcgctcctc ggcgagctcg atgacgagtc gctcatcgac
     1981 gacgagctcg acccgctcat caaaccggaa ctcgacccgc tcgcgctccc tgcggaggac
     2041 gaagaggagg aaacgaacaa gatattcgaa gacgaggagg aggacgagga ggacatggac
     2101 tacgactcgt tcgacgaccg agacgaggta tgacgaaaaa cgccgcgatc accgcggcgt
     2161 ttttcgtcgc gtgcgacgcg tctaggggac aaggcgtacg aatcgccgct tcccgacctt
     2221 gagcgtcact ggttcgctca cggattctgg gatcgcggaa agtttctccc ccgtctccgc
     2281 gttccgcaca ccgccggcct cgatgagccg ccggagttcc gtcttcgagg cgacgatgcc
     2341 cgccttgatg agttccgccg cgaaatcgcc gccgacctcg gggacatcct ccggtacgtt
     2401 ccccttttgg aacgtgtcga cgaacgcggc ttccgcggct tccgactcgg catcgccgtg
     2461 gtacatgcgc gtgatctcgc gagcgaggcg cattttgagg tcgcgcgggt tcgtgccact
     2521 cgcaagtgcg tccttcaccg cgtcgacctc gctcatgggg agtcgcgtgc acatcatgaa
     2581 gtacgaaaga agctcgctat cggcgataga catgaccttg ccgtacatat cggccgcatc
     2641 ggcggtgagc gcgatggtgt tcccccagct cgagctcatc tttctcccgt cagtgccgtt
     2701 taagagcggg ttcatgacga cgtcctgcgg ccgctcgccc gcatgctcct ggagcgtccg
     2761 tccggcgaga agattgaagc gctgatcggt gccgcctatt tcgacgtcgg cgcgaacgac
     2821 gacgctatcg tatccctgca tcaaggggta gaggacctcg cggagcgaca cgcgcttccc
     2881 cgcgtcgagc cgacgcttga tgttctcgcg cgcgatgaaa tcggcgaccg agaagaggtc
     2941 ggcgtgctcg cctatctcac ggtacgtgag cttctcgagc cactcggagt ttcgcctaca
     3001 ttcgacggcg tccatatcga gaatcttccc cgcttgttcg aggtacgtcg tgaggttctc
     3061 ctcgatgacg tcgcgcgtaa gcatcggccg ttcgctctcc ttgtccgacg tatcgccgat
     3121 ctgaccggtg aagtcgccga tgatgaacac gacctgatga ccgagttcct ggaagtcgcg
     3181 aagtttgagg agcgtgatgg cgcgcccgag atggatgtgc ggactcgtcg ggtcgatgcc
     3241 gagcttcacg cgaagccgct cgccggaggt gagccgcgct cggagcgact cctcgtggat
     3301 gatctccgcc acgccgcgcg tgaggaggtc gtctattttc atggcgtcgg tgcttggttt
     3361 catggggaat tcatcgcgat attggaataa ctgtatcaca ctcgcctcgc gtttatctat
     3421 acgcaataga atgctagtat ttgcaatatg acacgtttta cgacctgggc gaggaagcat
     3481 acgaaggaac tcttcatcga cctcaccatc atcttcctcg tcctcggtct catcggcgcc
     3541 ggcgtcgcgc tcgtctggat atcgacgctc gacatccccg acctctcctc gttcgaacag
     3601 agacgcgtcc aacaatcgac gaagatatac gaccggaccg gcgaggttct cctctacgac
     3661 ctccaccagg acgttcggcg taccgtcgtt ccgtacgagc ggatatcgcg acacatcaag
     3721 aacgcgacgg tcgctatcga ggacgacacg ttcttcgagc acaagggagt ccgcccgctc
     3781 gccatcctcc gcgcggcgat cgcgaacctc gagggcggcg atcttctcgg cggtcagggc
     3841 ggctcgacga tcacccagca ggtcatcaag aacgccgttc tcgagccgaa caagacgctc
     3901 tcgcgaaaga taaaggagat cattctctcc attcgactcg agcagatcat gtcgaaggag
     3961 gacatcctcg gcacgtacct caacgaggtc ccgtacggcg gcaccatcta cggcgtcgag
     4021 gaggcgtccg aggcgttctt cgggaagcat gcgtccgacg tgacgctctc tgaggccgcg
     4081 tacctcgcgt ctcttccgca actccccacg tactactcgc cgtacgggag ccacttcgac
     4141 gcgctcgaac gccggcacga cctcgtcctc gataagatgc tcgagcatgg tttcatcacg
     4201 gaggacgaat acaatcaggc gaaggcggag aaggtggcgt tccggccgca ggaggcgacc
     4261 ggcatcaagg c
//
LOCUS       DASTSP010000006        14012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1359090, whole genome shotgun sequence.
ACCESSION   DASTSP010000006 DASTSP010000000
VERSION     DASTSP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359090"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFS75_00120"
     CDS             <1..221
                     /locus_tag="VFS75_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU4677109.1"
                     /translation="PGLLTPAMVKAGAVVLDAGTSDEGGELRGDADPGVAEKASLFTP
                     VPGGIGPLTIAILLSNVVDCAEKNQAVV"
     gene            257..1672
                     /gene="secD"
                     /locus_tag="VFS75_00125"
     CDS             257..1672
                     /gene="secD"
                     /locus_tag="VFS75_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU4677110.1"
                     /translation="MSEQGGTKGDGLIRGFRALVVVVFAALVGYFVYTTTVHPDSKYP
                     FKLGLDLAGGSHLVYEADVSKLDPTEVPDAMNTLREVIERRINVFGVAEPVVQVERSS
                     IVAGKQAERLVVELPGVTNVDEAIKEIGKTPLLEFKLVDEEALAAQKAAENLASSTSG
                     AQISNVQVNGKDSSNPYIDTGLTGRYLKGAELVFGKGSATGLANEPTVSLTFNSEGAD
                     LFEQITSANVGKQLAIFLDGDIVSSPRINEGISGGHAVISGNFTADEARELVRNLNFG
                     ALPVPISLVSTQTIGSTLGDSALHAGMYAGIVGFIILGAFMIVWYRLPGVIAVIALIV
                     YVAAMLALFKLIPVVLTAAGIAGFILSVGLAVDANILIAERIKEEIRSGKHAQEAITE
                     GFSRAWLAIRDSNIAHIIVGIILFWFGTALIKGFALVFTIGVFVSMFSAITVSRTLLR
                     AIPIDVESKIGKFLLSSGLSR"
     gene            1674..2570
                     /gene="secF"
                     /locus_tag="VFS75_00130"
     CDS             1674..2570
                     /gene="secF"
                     /locus_tag="VFS75_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU4677111.1"
                     /translation="MNIKEHQGYLFAVPAIFATLSIAAILVWGLRPGIDVAGGSLLEV
                     SYDSRPPIESVNETVSKLDLGEIRVQASGENDYMLRQKELSQSEKDRLMEALNSLGAA
                     KEVQFNSVGPSIGAEIVRKSWWAIALVSLAIIAFIAFAFRHVSKPIASWKYGIVAIVT
                     LLHDILIPTGLFAYLGHSRDAEVGVLFIVAILTTLGISINDTIVVFDRIRENLRRNEE
                     RNRREPFTDVVWSSIFQTMARSINTSLTVIIMLAALYFLGPASTKDFSLTLIVGMIAG
                     TYSSIFFAAPLLTVIEKRQRKA"
     gene            2737..3291
                     /locus_tag="VFS75_00135"
     CDS             2737..3291
                     /locus_tag="VFS75_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4677112.1"
                     /translation="MIIGIAGSFGAGKGAVVDYLKKKGFTHYSASAFITEELTRLGMP
                     VNRDSMIEVGNRLRKEHGPAYIIETLYDDAKKAGGDAVIESLRAVAEVRKLKELGAVV
                     IGVDAAPEVRYERAVSRGSEKDKVSFEHWLTQERQESNPDDPTKQDIWGALKEADHLI
                     ENNGSIGDLHRTVDAFLAEMRHEG"
     gene            complement(3288..3992)
                     /gene="pyrH"
                     /locus_tag="VFS75_00140"
     CDS             complement(3288..3992)
                     /gene="pyrH"
                     /locus_tag="VFS75_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU4677113.1"
                     /translation="MMHTPPETVVMSVGGSLIVPDQIDCLFLADLKNLVEHQHTSFGR
                     RFVIIAGGGKTARRYQDAAATVTELDPEDLDWMGIHATRLNGHLLRTIFREIAYPEMI
                     TNPDDVRNVPSDAPLIIAAGHRPGASTDLRAVQIATITGARKLVNLSNIDYVYTDDPK
                     QNPHAEKIADIRWDDFMKLIPTEWNPGLSSPFDPVAAREAKAHGLEVSIINGARLDEL
                     EHYLDGRPFMGTRIHA"
     gene            4496..5955
                     /locus_tag="VFS75_00145"
     rRNA            4496..5955
                     /locus_tag="VFS75_00145"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            6259..8520
                     /locus_tag="VFS75_00150"
     CDS             6259..8520
                     /locus_tag="VFS75_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677114.1"
                     /translation="MKKLHLALLFALFFVGVHTASADTASDWMAQNCEDLNFGEYGLI
                     TPMLYVTPDGTQDLIQIGEPVTFSVHGNLTENVDLSFSDSAITMGQKQCLDGSAYGAD
                     GCPQLGQCTGTGTSTQCHQRYNFKQTATLTGTPRDIVVKVPNGGMRAYNGASGKSVCQ
                     LLHVRRERQTYTLGLGIDKPAFGAKEAFKVTLSGMSWSKAKEVFGTNPPFSFTRSNAP
                     WTSCENGIFRNSVGGGCSGDSVLSCGLLPDQSAPQYLCAVTGTGALPNSATDVIYTAY
                     IPKLQDQFDTYAYDNGDQSITLTKLGQGNDGGMTNYMNCPAGQGVDPSSPSGGCTRCQ
                     NIANDFKSHISSCGSAQDSYKWREQTYSDFYRNYSVCDVSVTDGSACTTVGAMCIKHD
                     PDRAFECKNTVGESYTQYYGRVIDGASGGTKGLAGVTVNPHHITAGYLGFDCSKLTTT
                     TDADGNFRFSDSDSKSLCLTLAGASVKYSKDGYFDATGAVGDEFDRMIPTVTMLPLSG
                     SDQTTQTGPLSIDNGNQTMNGNDVVFTIHNAIQGQVEACYQVLHHPNTGVNTDPNTPC
                     SEWAPFNGNDEWHFHPENQTLVGTLGVNAVDKNGNPVWNIPGLQVRSSFRKVGTTDVV
                     TATMTVAGNAQQGNNDGNGESSGHWTHTAGHFSPMPPACTSTPTDGAACSPIGSACST
                     ANDDWSCSGAKSGQAGPEAQAEPQKPGLIENAINRIGRVGGAIGDSFIRIGGYTGSFF
                     RGMLVNFGIVLSE"
     gene            complement(8584..8886)
                     /locus_tag="VFS75_00155"
     CDS             complement(8584..8886)
                     /locus_tag="VFS75_00155"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677115.1"
                     /translation="MDLQTLFTNLIAFTNATLIPFLLAIAFLVFIWNVVRYFIIGGAD
                     EGEHEHARSLATWGILAFVVILSLWGIVNLLVDGLGLADPTPVTPDYIGWQEAPGP"
     gene            8994..9242
                     /locus_tag="VFS75_00160"
     CDS             8994..9242
                     /locus_tag="VFS75_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677116.1"
                     /translation="MKTKKRHSLLSVMRFLAFLLVLGATALLIERSTPPAGDVVAAEE
                     ARIEQAAENPKSREAKYLACSHGKTACDAYIAHRSKGR"
     gene            complement(9343..9519)
                     /locus_tag="VFS75_00165"
     CDS             complement(9343..9519)
                     /locus_tag="VFS75_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677117.1"
                     /translation="MTCWTQGGLVLRASGAREQEGTPHEPLSDEDRARSEKRRARKRF
                     ERRRKRAANPKKSS"
     gene            9810..9882
                     /locus_tag="VFS75_00170"
     tRNA            9810..9882
                     /locus_tag="VFS75_00170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9843..9845,aa:Ile,seq:gat)
     gene            9924..11270
                     /gene="murD"
                     /locus_tag="VFS75_00175"
     CDS             9924..11270
                     /gene="murD"
                     /locus_tag="VFS75_00175"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU4677118.1"
                     /translation="MNDLGAYFKGKKITVMGLGLLGRGVGDSAYLARAGAELIVTDMK
                     PAADLKESLQRLSSYPNITYVLGEHRMEDFIGRDIILKAAGVPLDSPYIAAARKDGAK
                     IAMSGALFAKLSGVPVIGVTGTRGKSTVTHMIDHVLRHATEGAAVLLGGNVRGVSNLE
                     LLERVEEGSLAVMELDSWQLQGFGEERMSPHISVFTNFMEDHMNYYKGDMEAYFADKA
                     NIFRYQEQADVLVTTRAVFERAEAFMRETGEELTMDVRLVDASILPEDCLLSMPGEHN
                     RLNAALAMEALRATSLTDEEIVDGLASFPGVPGRLEYLGTIDGVKVYNDNNATTPQAT
                     SEGLRAVADGKNIVLIAGGADKNIDLAPLVSAIGDHAKAVVLLPGTGTERLRGRLDAR
                     VAKTLEEAVEEALRLASPGDVVLFSPGFASFGIFKNEYDRNDQFVNAVHERQAREL"
     gene            11275..12567
                     /gene="murC"
                     /locus_tag="VFS75_00180"
     CDS             11275..12567
                     /gene="murC"
                     /locus_tag="VFS75_00180"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU4677119.1"
                     /translation="MNFKHIHFIGVGGIGMSALARLFLHDGKMVSGSDRAPSDITRAL
                     ERLGMHFYPTQTGSNVTDDVDLVVYTEAMPRDHEELVEARERGIRTMNYFSALGMIAN
                     EYYLVAVAGSHGKTTTTAMLIDIFERANLDPSAVVGSLRASTKSNYRAGKGKYFIAEA
                     CEYRRDFLSLRPDILVITNIEHEHVDYYPDLASVQAAFREFALQVKEGGAIIADLANP
                     SVRPVVEGLSVTVIDYGKYFDPALSLSVPGMHNQMNAAAAKAAAVFAGVKPDAAKEAL
                     ENFAGTWRRFEYKGEVNGAKVYDDYGHHPTEIAATIRGARELYPDRKLTLVFQSHTYS
                     RTHTLFDDFVSALSLADRTLVLPIYAAREENESGVSHTKLAEAIRMRGKVAEACDSFE
                     GVVSDLRAHASAKDLILVMGAGDVTKVAGMLTNTDFTS"
     gene            12616..13161
                     /locus_tag="VFS75_00185"
     CDS             12616..13161
                     /locus_tag="VFS75_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677120.1"
                     /translation="MARRTYVVVLGSGTRDGALSLERREWLAKKTSFQGVLLNDHAEY
                     RVIRGIDRIRVLQQRGEEVGGMLFTSGFDGRITAETIAEAMARYANAIFIEAERDEHG
                     TLMRVPLIANDDGRVRGLRKEMAWACDRVHETCGILYRTRLEFVAEPWETFLVPIVNY
                     FAMRMPDCWALPVREPSILGN"
     gene            13239..13922
                     /gene="rsmI"
                     /locus_tag="VFS75_00190"
     CDS             13239..13922
                     /gene="rsmI"
                     /locus_tag="VFS75_00190"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU4677121.1"
                     /translation="MAGKLSIVATPIGNLEDVTLRALRTFKEAEYVLCEDTRVTGKLT
                     ARHGIKASLKRYDAHASERVHKAILADLASGKHIALATDAGTPGISDPGVFLVARVRR
                     ELPQVTIEAIPGPSAVTAAFSIAGIPGNAFTFLGFAPQKKGRATFFKRLAAYEVPVIF
                     YESPHRIAKALESLAAELPTDFRVTVARELTKLHEEVIAGSPAEVRDYFTEHPDHVRG
                     EFVIIVSRG"
BASE COUNT         2910 a         4135 c         4253 g         2714 t
ORIGIN      
        1 tccccggtct ccttacgccc gcgatggtca aagcgggcgc cgtcgtcctc gacgcaggta
       61 cctccgacga gggaggggag ctccgcggtg acgcggaccc aggagtcgca gagaaggcat
      121 ctctctttac ccccgttccg gggggcattg gccccctcac tattgccatc ctcctctcga
      181 atgtggtgga ttgcgccgag aaaaaccagg ctgtggtata gtgttcccca tatttagcca
      241 gctaagtgag atttccatga gcgaacaagg aggcacaaag ggcgacggac tcattcgcgg
      301 gtttcgggcg ctcgtcgtcg tcgtattcgc ggcgctcgtc gggtacttcg tctacacgac
      361 gaccgttcat ccggattcga agtatccctt caagctcggg ctcgacctcg cgggcggcag
      421 tcacctcgtc tacgaggcgg acgtctcgaa gctcgacccg acggaggttc ccgacgcgat
      481 gaacacgctc cgtgaggtca tcgaacggcg tatcaacgtc ttcggcgtcg cggaaccggt
      541 cgtgcaggtc gagcgctcga gcatcgtcgc ggggaagcaa gccgagcgcc tcgtcgtcga
      601 gcttcccggc gtgacgaacg tcgatgaagc catcaaggag atcggtaaga cgccgcttct
      661 cgagttcaag ctcgtcgacg aggaggcgct cgcggcgcag aaagctgccg agaatctcgc
      721 gtcatcgacg tccggcgcgc agatatcgaa cgtccaggta aacggcaagg attcgtcgaa
      781 cccatacatc gatacgggcc tcacggggag gtaccttaag ggcgccgaac tcgtcttcgg
      841 aaaaggctcc gcgaccggtc tcgcgaacga gccgacggta tcgctcacgt tcaatagtga
      901 gggcgccgat ctcttcgaac agatcacctc ggcgaacgtc gggaagcaac tcgccatctt
      961 cctcgatggc gatatcgtct cgtcgccgcg catcaacgag ggtatttcgg gcggccatgc
     1021 ggtcatctcg ggcaacttca ccgccgatga ggcacgtgag ctcgtccgta acctcaactt
     1081 cggcgcgctc ccggtgccca tctcgctcgt ctcgacgcag accatcggtt cgaccttggg
     1141 tgacagcgcg ctccacgcgg gtatgtacgc cggcatcgtc gggttcatca tcctcggcgc
     1201 cttcatgatc gtctggtacc gtctcccggg cgtcatcgcg gtcatcgcgc tcatcgtgta
     1261 cgtcgcggcg atgctcgccc tcttcaagct catcccggtc gtcctcaccg cggcaggcat
     1321 cgccggcttc atcctctcgg tcggcctcgc cgtcgacgcg aacatcctca tcgccgaacg
     1381 catcaaggag gagatacgtt cggggaagca cgcgcaagag gcgataaccg agggtttcag
     1441 ccgcgcgtgg ctcgccatcc gcgactcgaa catcgcgcac atcatcgtcg gtatcattct
     1501 cttctggttc ggcaccgcgc tcatcaaggg attcgcgctc gtcttcacga tcggcgtatt
     1561 cgtctcgatg ttctccgcga tcaccgtctc ccgcacgctt cttcgagcca tcccgataga
     1621 cgtcgagagc aagatcggga agtttttact cagttcaggc ctctctcgat aatatgaaca
     1681 taaaagaaca tcaggggtac ctcttcgccg tcccggcgat cttcgcgacg ctctcgatcg
     1741 ccgctatcct cgtctggggc ctccgtcccg gcatcgacgt ggccggcggt tcgctccttg
     1801 aggtctcgta cgattcgcgt ccgccgatcg aatcggtgaa cgaaacggtc tcgaagctcg
     1861 acctcggcga gatacgcgtg caggcctcgg gtgagaacga ctacatgctc cgtcagaagg
     1921 aactctctca gagcgagaag gacaggctca tggaggcgct caactcgctc ggcgcggcga
     1981 aggaggtgca attcaactcg gtcggcccct cgatcggcgc ggagatcgtc cgaaaatcgt
     2041 ggtgggccat cgcgctcgtg tcgctcgcca tcatcgcgtt catcgcgttc gcgttccgtc
     2101 acgtctcgaa gccgatcgcg tcgtggaagt acggcatcgt cgccatcgtc acgctcctcc
     2161 acgacattct catcccgacc ggcctcttcg cgtacctcgg ccactcgcga gacgcggagg
     2221 tcggtgtcct cttcatcgtc gccatcctca cgaccctcgg tatctcgata aacgacacca
     2281 tcgtcgtctt cgaccgcatc cgcgagaacc tgcgacgtaa cgaggagcgg aatcgccgag
     2341 agccgtttac cgacgtcgtg tggtcgagta tcttccagac catggcccgg tccatcaaca
     2401 cgtccctcac cgtcatcatc atgctcgccg cgctctactt cctcggcccg gcatcgacga
     2461 aggacttctc gctcacgctt atcgtcggta tgatcgccgg tacgtactcg tcgatattct
     2521 tcgcggcgcc gctcttaacc gtcatcgaga aacgtcagcg gaaggcatga tcatcggaat
     2581 cgctggttcg ttcggagcgg ggaagggtga gacagtccgg gggactgtcg caagaggtcg
     2641 tcgatatttt caggagaaaa tatcatacga cctgaacagc gccggtaagg cgcccgcgcc
     2701 catctgtact ttatttagtg gaagcacggc aagtctatga tcatcggaat cgctggttcg
     2761 ttcggagcgg ggaagggcgc ggtcgtcgac tacctaaaga agaagggctt cacgcactac
     2821 tccgcgagcg cgttcataac cgaggaactc acgcgcctcg gcatgccggt caaccgcgac
     2881 tccatgatcg aagtgggcaa ccggcttcga aaggagcacg gcccggcgta catcatcgag
     2941 acgctctacg atgatgcgaa gaaggcgggc ggcgatgccg tcatcgaatc gcttcgcgcg
     3001 gtcgccgagg ttcgaaagct caaggagctc ggcgcggtcg tgatcggcgt ggacgctgcg
     3061 cccgaggtcc gctatgaacg cgccgtctca cgggggagtg agaaggacaa ggtgagcttc
     3121 gagcactggc tcacgcagga gcgacaggaa tcgaatcccg atgacccgac gaagcaggat
     3181 atttggggcg cgctcaagga agcggaccat ctcatcgaga ataacggctc gatcggcgac
     3241 ctccaccgca ccgtcgatgc gttccttgcc gagatgaggc acgaaggtta ggcgtggatg
     3301 cgcgtgccca tgaaggggcg gccgtcgagg tagtgctcaa gttcgtctaa gcgcgcgccg
     3361 tttatgatac tgacctcgag gccatgcgct ttcgcctcgc gcgcggcgac cgggtcgaac
     3421 ggagaggaga ggccgggatt ccactcggtc ggaatgagtt tcatgaaatc gtcccagcgg
     3481 atgtccgcta tcttctcggc gtgaggattc tgcttcgggt catcggtgta gacgtagtcg
     3541 atgttcgaga ggtttacgag ctttcgcgca cccgttatcg tcgcgatctg gacggcacgg
     3601 agatcggtcg acgcaccggg ccgatggccg gccgcgatga tgaggggcgc atcgcttggg
     3661 acgtttctga cgtcgtcggg attcgtgatc atctcggggt acgctatctc gcgaaaaatg
     3721 gtacggagga gatggccatt gagtcgggtc gcatgaatgc ccatccagtc gagatcctcg
     3781 gggtcgagct cggtcaccgt cgcggccgcg tcctggtacc ggcgtgccgt cttgccgccg
     3841 cccgcgatga tgacgaaacg gcgaccgaag ctcgtatgct ggtgttcgac gaggttcttg
     3901 aggtcggcga ggaagagaca atcgatctgg tcgggaacga tgagggagcc gccgaccgac
     3961 atgacgaccg tctcaggggg agtgtgcatc atcgatagtg taggcggcgg cgcgaggaac
     4021 cgcaaccgct cgcccgggat atgcccgttt tccgctcttt tttggccgga gcctctccgg
     4081 tcccgcgtgc gacggtcctc gatgctcatt tcgcgaggaa ccgcttgacc ttttatgcga
     4141 gacctgtata atagcgccac tgtccatttt gggttgaggc atagagcgcc tagaaggcgt
     4201 tttacgtttc atccagaagg tggaggacat cgttttttga aaatcgaata accgaataca
     4261 tatgtagcaa cccatatgta ttcgtacgta tagttacgct cgaaagagcg tgatggtacg
     4321 catcagctag aatgtgaaaa cattcgaacg aaagacaaaa gcgaacatgt tcgaaagaac
     4381 gtgcactctc ttttgtctta tccttggaat gtctcgaagg gaaacgaata gtacagtttc
     4441 cgcatacttc gagacgacaa gatcaacgag tcacttcggt gactcagatc ctgtttaaac
     4501 ttagagtttg atcctagctc agggtgaacg ctggcggcgt ggatgaggca tgcaagtcga
     4561 atgggttcag acccatggca gacgaggtag gaacacgtag gtacataccc tcaagtcacg
     4621 gataatctgc cgaaaggcag gataatacgt gatggtccct tcggggtaaa gatttatcgc
     4681 ttgaggaatg gcctgcgttc tatcagctag ttggtacggt aacggcgtac caaggctacg
     4741 acggataggg gaggtgagag cctgaccccc accgatggaa ctgagacacg gtccttactc
     4801 ctacgggagg ccgcagtcga gaatcttccg caatggacga aagtctgacg gagcgacgcc
     4861 gcgtggtgga tgaagtccct agggacgtaa acacctttta tgagggagga agtttattga
     4921 cgttacctca tgaataaggg gctcccaatt ctgtgccagc aggagcggta atacagaagc
     4981 cccgagcgtt acccggattt actgggcgta aagggtgtgt aggtggtgtg attagtcttg
     5041 tgttaaatct ccgggctcaa cccggaactc gcgcgagaaa cggtcacact agaggaagtg
     5101 agaggtgtgc ggaactcatg gtgtaggggt gaaatccgtt gatatcatgg ggaacaccga
     5161 aagcgaaggc agcacactgg cactctcctg acactgaaac acgaaagcgt gggtagcgaa
     5221 tcggattaga tacccgagta gtccacgccc taaacgttgt ccgctagcta tagggagtat
     5281 cgaccctctc tgtggcgtag ctaacgcgat aagcggaccg cctgggtagt acgagcgcaa
     5341 gcttaaaact caaaggaata gacgggggct cgcacaagcg gtggatcatg gggcttaatt
     5401 cgtcactaag cgaggaacct taccaaggct agaaaccata ctgcacgcct cgtgaaagcg
     5461 aggaagcctt cgagggtgta tggcaggtga tgcatggccg tcgtcagttc gtggcttgag
     5521 ctgttccctt aagtggggaa acgaacgcaa ccctcgttgc ctgttacaag tgtcaggcga
     5581 gactgctccc tcacgggaga ggaaggtgag gatgacgcca ggtcagcatg tccctagatg
     5641 ccttgggctg cacccgtgat acaatgggtg gtacaacgag acgcaatgcc gcgaggcgga
     5701 gcaaatcttt ataaaccacc ctcaattcgg attgaagtct gcaactcgac ttcatgaagt
     5761 cggaatcgct agtaatcgtg gatcagcaac gccacggtga atacgttccc gagccttgta
     5821 ctcaccgccc gtcaactcaa gggagccgga agtgcccgaa gctcgcgctc gtcgcgatct
     5881 acggtaagtt cggtgacagg gagtaagtcg taacaaggca cggctagcgg aagctggtcg
     5941 tggattaatt ccaattggac acgtcgttgg tccggggtaa cccgaactat gaaacggcta
     6001 attggtcgac cttttgtgcc tcaattgagc acaaaagaag ggatgatacc caaccttcaa
     6061 aacggtatct ggacaccgcg gaaagacgcg gccaggataa gacaaaagag agtgagaacg
     6121 ctgatgaaac atgaaaaggc caccctaacg gtggcctttt catgtttcat cagtgaaaag
     6181 cgccttcgcg ctttttcgtg cgtcgctgtg cataaacgcg ctgtctcgcg cttaccgcgg
     6241 gcggtatcat gtacccatat gaaaaaattg catctcgcgc tccttttcgc gctcttcttc
     6301 gtcggcgtac acacggcgag cgccgacacg gcgagcgatt ggatggcgca gaactgcgaa
     6361 gatctcaatt tcggcgagta cgggctcatc acgccgatgc tctatgtgac gccggatggg
     6421 acgcaggacc tcattcaaat aggcgagccg gtcacgttct ccgtccacgg gaacctcacc
     6481 gagaacgtcg acctctcttt cagcgactcc gccataacga tggggcagaa gcaatgcctc
     6541 gacggcagtg cgtatggcgc cgacggctgt ccgcagctcg gacagtgtac gggaacgggt
     6601 acgtccacgc agtgtcacca gcggtacaat ttcaagcaga cggcgacctt gaccggcaca
     6661 cctcgggaca tcgtcgtgaa ggtcccgaac ggcggcatgc gcgcgtacaa cggtgcctcg
     6721 ggtaagtcgg tgtgccagct cctccatgtc cgcagggagc gacagacgta taccctcggt
     6781 ctcggtatcg acaagccggc gttcggtgcg aaagaggcgt tcaaggtgac gctctccggt
     6841 atgtcgtggt caaaggcgaa ggaggtcttc ggcacgaacc cgcctttcag tttcacgcgg
     6901 tcgaacgcgc cgtggacgag ctgtgagaac ggcatattcc gaaacagcgt gggtggaggt
     6961 tgctcggggg acagcgttct ctcatgcggg cttctgcccg atcagtccgc accgcagtat
     7021 ttgtgcgcgg tcaccggaac gggcgcattg ccgaacagcg cgaccgacgt catctacacc
     7081 gcgtacatcc ccaaattgca ggaccagttc gacacgtacg cgtacgataa cggcgaccaa
     7141 tccattacgc tcacaaagct cggacagggc aacgacggcg gcatgaccaa ctacatgaac
     7201 tgtcccgcgg ggcagggagt cgacccgtct agcccgagtg gaggttgtac gcgctgtcag
     7261 aacatcgcga acgatttcaa gtcgcatata tcgtcgtgtg gaagcgccca agactcgtac
     7321 aagtggcgag aacagacgta cagcgacttc tataggaact atagcgtctg cgacgtgagc
     7381 gtcacggacg ggtcggcctg tacgacggtt ggtgctatgt gcatcaagca cgaccccgac
     7441 cgcgcgttcg agtgtaagaa caccgttggt gaatcgtaca cgcagtacta cgggcgcgtc
     7501 atcgacggcg cctcaggcgg cacgaagggc ctcgcaggcg tcacggtgaa tccccatcac
     7561 ataacggccg gatatctcgg cttcgattgc tcgaaactca ccacgacgac ggacgcggac
     7621 ggcaacttta gattctccga cagtgacagc aagtccctct gtctcaccct cgccggtgcg
     7681 agcgtcaaat actcgaagga cggatacttc gacgcgacag gggccgtggg tgacgagttc
     7741 gaccgaatga taccgacggt cacgatgctc cccctaagcg gctccgacca aacgacgcaa
     7801 accggcccgc tttccattga taacggcaat cagacgatga acgggaacga cgtcgtcttc
     7861 accatccata atgcgattca gggtcaggtc gaggcgtgct atcaggtgct ccatcacccg
     7921 aacaccggcg tcaataccga cccgaatact ccttgttcgg aatgggcacc gtttaatgga
     7981 aacgacgagt ggcattttca tcccgagaat caaacactcg tcggaacctt gggtgttaac
     8041 gcggtcgaca agaacggcaa cccggtttgg aacatccctg gactccaagt ccgttcatcc
     8101 tttaggaagg tcggtacgac cgacgtcgtc accgcgacga tgacggtggc cggcaatgcc
     8161 cagcagggga acaatgacgg caatggtgag tcctcggggc actggacgca tacagcggga
     8221 cactttagcc ctatgccgcc tgcatgtaca tcgacgccga cggatggtgc cgcgtgctcg
     8281 cctataggtt ccgcttgttc gacggcgaac gacgactgga gctgcagtgg tgcgaagtcg
     8341 ggccaagcgg gaccggaggc gcaggcagag ccccagaagc ccggcctcat cgagaatgcc
     8401 ataaacagga taggacgcgt cggcggcgcg atcggcgaca gcttcatcag aataggaggc
     8461 tacaccggca gtttcttccg cggcatgctc gtgaacttcg gcatcgtact ctcggagtag
     8521 tatgaccata tgaaaaaccg ccgcgaccac gtcgcggcgg tttttcgctc gtgcgcgtat
     8581 cgtctagggt cccggtgcct cttgccagcc gatgtagtcg ggcgtcaccg gggttgggtc
     8641 cgcaagacct aagccgtcga ccagaaggtt cacgatgccc cagagggaga ggatgacgac
     8701 gaacgcgagg atgccccacg tcgcgagcga gcgcgcgtgt tcgtgctcgc cttcatccgc
     8761 gcccccgatg atgaaatacc gaacgacgtt ccagatgaac acgagaaacg cgatcgcgag
     8821 gaggaaggga atgagcgtcg cgttcgtgaa cgcgatgaga ttcgtaaaga gcgtctgaag
     8881 gtccatgtac caatagtacg ctaaaaaacc gcgaacaggc gcggtttcta cacattgaca
     8941 aatatatcaa ttactgtcat aataatagat agattccaac aagggagagc accatgaaga
     9001 cgaagaagcg gcactcgctt ttgtcggtca tgcggttcct cgcgtttctc ctcgtactcg
     9061 gtgcaaccgc gctcctcatc gagcgctcga cgccgcccgc gggcgacgtc gtcgccgcag
     9121 aggaggcgcg catcgagcag gccgcggaga accccaagag ccgcgaggcg aagtacctcg
     9181 cgtgctcgca cgggaagacc gcgtgcgacg cgtacatcgc ccaccgctcg aagggtcgct
     9241 gacagagcga ctccatcggc aaacgtcgga cctcgtgtcc ggcgtttttc tttcacggcc
     9301 acgcaggaaa agggccggcg cgcatacgcg ccggcccatc gttcacgatg acttcttcgg
     9361 attcgccgct cgcttcctcc gccgttcgaa gcgcttccgc gcgcgtcgct tctcgcttct
     9421 cgcacggtcc tcgtcggaga gcggttcgtg gggcgttcct tcctgttctc gcgcaccgct
     9481 cgcgcggagc acgagtcctc cctgcgtcca gcaggtcata tgtggcttct ccttgattat
     9541 ggggtacggt ggtgccggag atacagtacc ctaggtcggc cggccgtggc aagccgccct
     9601 tgttttcatt gcccaaatgg acgttttttg gtagtatcgg tgggcttaaa ggggttcaac
     9661 cctctgtccg cgcttagctc agttctagag tgagatcggt gggacccgat cgcaagacct
     9721 tgagctttac gaacggtata ccgttcccgt aaggaatcga ctgacttcct acagaggctg
     9781 tatactttca gtacttttgt tcattttgtc cgcgcttagc tcagttggta gagcattcga
     9841 ctgatactcg aaaggtctta ggttcaaatc ctaaagcgcg gacaaagttc gcaaatgagc
     9901 tcccgagaca tcgggtcaca cgtatgaacg acttgggcgc atacttcaaa gggaagaaga
     9961 taaccgtcat gggactcggg ctccttggac gtggcgtcgg cgacagtgcg tacctcgcgc
    10021 gcgccggggc ggagctcatc gtgaccgaca tgaagcctgc ggcggacctc aaggaatcgc
    10081 tccagcgtct ctcgtcgtac ccgaacatca cgtacgtgct tggcgagcat cgcatggagg
    10141 acttcattgg ccgggacatc atcctcaaag cggccggcgt gcccctcgat tctccgtaca
    10201 tcgcggccgc acggaaggac ggcgcgaaga tcgcgatgag cggcgcgctc ttcgcgaagc
    10261 tctccggtgt tcccgtgatc ggcgtgaccg gcacgcgcgg gaagtcgacg gtcacccata
    10321 tgatcgacca cgtcctccgt cacgcgaccg agggcgcagc ggtccttctc ggtgggaacg
    10381 tccgcggcgt ctcgaacctc gagctcctcg aacgcgtcga ggaagggtcc ctcgccgtca
    10441 tggagctcga ctcgtggcag cttcagggct tcggcgagga gcgcatgagc ccgcatatct
    10501 ccgtcttcac gaacttcatg gaagaccaca tgaactacta caagggcgat atggaggcgt
    10561 acttcgccga caaggcgaac atatttcgat accaagagca ggcggatgtc ctcgtgacga
    10621 cgcgcgccgt cttcgagcgg gcggaggcgt tcatgcggga gacgggtgag gaacttacca
    10681 tggacgttcg cctcgtcgac gcgtctatcc tccccgagga ctgcctcctc tccatgccgg
    10741 gcgagcataa ccgcctcaat gccgcgctcg cgatggaggc cctccgcgcg acgagcctca
    10801 ccgacgagga gatcgtggac ggcctcgcga gcttcccggg cgtaccgggc cgcctcgaat
    10861 acctcgggac gatcgacggc gtgaaggtat acaatgataa caacgcgacg acgccacagg
    10921 cgaccagcga gggcctccgg gccgtcgcgg acggcaagaa catcgtgctc atcgcgggcg
    10981 gagcggataa gaacatcgat ctcgcgccgc tcgtgtccgc gatcggggac cacgcgaagg
    11041 cggtcgtgct cctccctgga acggggacag agcgacttcg tggacgatta gacgcgcgcg
    11101 tcgcgaagac cctcgaggag gcggtcgagg aggcgcttcg gctcgcgtcg ccgggcgacg
    11161 tcgttctttt ctctcccggc ttcgcgtcct tcggcatttt caagaacgag tacgaccgga
    11221 acgaccagtt cgtgaacgcg gtgcacgagc ggcaggctag agaactttag gagcatgaac
    11281 ttcaagcata tccatttcat cggcgtcggc ggcatcggga tgtcggcctt ggcacgcctt
    11341 ttccttcacg acggaaagat ggtgagcggg tccgaccgcg cgccgtccga tataacgcgt
    11401 gcgctcgagc ggctcggtat gcacttctac ccgacgcaga cgggttcgaa cgtcaccgat
    11461 gacgtcgacc tcgtcgtcta caccgaggcg atgccgaggg accatgagga gctcgtcgag
    11521 gctcgcgagc gcggtatcag gacgatgaac tactttagtg cgctcggcat gatcgcgaac
    11581 gagtattacc tcgtcgcggt cgcggggagt cacgggaaga cgacgacgac cgcgatgctc
    11641 atcgacatct tcgagcgcgc gaatctcgac ccttcggccg tcgtcgggtc tttgcgcgcg
    11701 tcgacgaagt cgaactatcg ggcggggaag gggaagtact tcatcgccga ggcgtgcgag
    11761 tatcgccgcg atttcctctc gcttcgcccc gacatcctcg tcatcacgaa catagagcac
    11821 gagcacgtcg actactatcc ggacctcgcg tcggtccaag ccgcgttccg cgagttcgcg
    11881 ctccaggtta aggagggcgg tgcgatcatc gccgacctcg cgaacccgag cgtgcgaccg
    11941 gtcgtcgagg gtctctcggt caccgtcatc gactatggga agtatttcga tccggcgctc
    12001 tcgctctcgg tccccggtat gcacaaccag atgaacgccg ccgcggcgaa ggccgcggcg
    12061 gtcttcgccg gcgtgaagcc ggatgccgcg aaagaggcgc tcgagaactt cgcgggcacg
    12121 tggcgtcgct tcgagtataa gggagaggtg aacggcgcga aggtgtacga cgactatggc
    12181 caccacccga cggagatcgc ggcgacgata cgcggcgcgc gcgagctcta tccagaccgg
    12241 aaactcacgc tcgtctttca atcgcatacg tactcgcgga cacatacgct attcgatgat
    12301 ttcgtctccg cgctatccct cgcggatcga acgctcgttc tcccgatcta cgccgcacgg
    12361 gaggagaacg agagcggcgt ctctcatacg aagctcgccg aagcgatacg catgcggggg
    12421 aaggtcgccg aggcgtgcga ctcgttcgaa ggcgtcgttt ccgacctgcg tgcccatgcg
    12481 agcgctaagg acctcattct cgtgatgggg gccggggacg tgacgaaggt cgcgggcatg
    12541 ttgacaaata ccgattttac ttcataatta aatttagaag ctcactaaaa cccgtattcg
    12601 acaggaggat aagacatggc ccgaaggacg tacgtcgtcg tcttgggaag cggaacgcgt
    12661 gacggggcac ttagccttga acggagggaa tggctcgcga agaagacctc tttccagggg
    12721 gtcctcctca acgaccacgc ggagtatcgg gtcatccgtg gcatcgaccg catccgcgtg
    12781 cttcagcaaa gaggagagga ggtcggtggc atgctcttca cctctggctt cgacggccgc
    12841 atcacggcgg agaccatcgc cgaggcgatg gcgcgctatg cgaacgccat cttcatcgag
    12901 gcagaacgcg acgagcatgg gacgctcatg cgcgtaccgc tcatcgcgaa cgacgacggt
    12961 cgcgtgcgcg ggctccggaa ggagatggcg tgggcttgcg accgcgtgca cgaaacgtgc
    13021 ggtatcctct accgcacgcg tctcgagttc gtcgcggaac cgtgggagac cttcctggtc
    13081 cccatcgtga actacttcgc catgcggatg cccgattgct gggcactccc ggtgagggaa
    13141 ccgtccatcc tcggcaacta ggacgtcgaa atgcggtagt gaagcggcac cctcgggtgc
    13201 cgcttttttc tttgccgcgc ccgtggtagc gtgtggccat ggcagggaaa ctctccatcg
    13261 tcgcgacgcc gatcggcaat ctcgaggacg tgacgctccg cgcgctccgc acattcaagg
    13321 aggccgagta cgtcctctgt gaggacacgc gggtgacggg gaagctcacg gcgcgccacg
    13381 gcatcaaggc ttcgctcaaa cgctacgacg cgcacgcgag cgagcgagtg cacaaagcga
    13441 tactcgccga cctcgcctcg gggaagcaca tagcactcgc gaccgacgcg gggacgccgg
    13501 gcatcagcga tccgggcgtc ttcctcgtcg cgcgcgttcg aagggaactt ccccaggtca
    13561 ccatcgaggc gataccgggg ccgtctgcgg tgaccgccgc gttctcgatc gccggcattc
    13621 cggggaacgc gttcacgttc ctcggcttcg caccgcagaa gaagggccgc gcgacgttct
    13681 tcaagcgcct cgcggcgtac gaggtaccgg tcatcttcta cgagtcaccg caccgcatcg
    13741 cgaaggcgct cgaatcgctc gcggccgagc tccccacgga cttccgcgtc acggtcgccc
    13801 gcgagctcac gaagctccac gaggaggtca tcgcaggatc gcccgcagaa gttcgggatt
    13861 atttcaccga gcaccccgac cacgtccgcg gcgagttcgt catcatcgtc tcgcgcggtt
    13921 gacaacgctt tttcaattca agcttacacg gtatactcta cgcatgtcta aggaatcctt
    13981 cgtcctcctc atcggtatca tcgttttctt cg
//
LOCUS       DASTSP010000007        12853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_914703, whole genome shotgun sequence.
ACCESSION   DASTSP010000007 DASTSP010000000
VERSION     DASTSP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12853
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914703"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /gene="rplU"
                     /locus_tag="VFS75_00195"
     CDS             <1..191
                     /gene="rplU"
                     /locus_tag="VFS75_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU4677122.1"
                     /translation="LVDDGKDATIGMPYIKGATVKGTFEGVKKGKKITIVRYKAKSNR
                     DRKIGHRQKHAAVRIEAI"
     gene            complement(222..833)
                     /locus_tag="VFS75_00200"
     CDS             complement(222..833)
                     /locus_tag="VFS75_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU4677123.1"
                     /translation="MTPFDKLARYFEKFPGIGSRQARRFVFHLLTMGESDLREFAELI
                     RTIKDEVTECPSCHRFHSGRASERCALCESAVRDRSKLLVVERDTDIDAIERSGVYDG
                     LYFVLGGTVPLLGTEEVKGLRGGALKHTVGERAKEGLSEVILGFSVNPDGENTGRYVE
                     SLLHELLAANPIKLSHLGRGLSTGSELEYADPETIKNSLKHRE"
     gene            complement(840..2240)
                     /gene="dnaB"
                     /locus_tag="VFS75_00205"
     CDS             complement(840..2240)
                     /gene="dnaB"
                     /locus_tag="VFS75_00205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU4677124.1"
                     /translation="MDETREDVKQTATKSRLLRTPPQNLDAERALLGAIMLKPEAMHD
                     VSVTVFPESFYADKHREIYRAILDTFMHGDPIDLLSVTTKLKTRNLLERVGGASYVTE
                     LVESVPAAGNAIYYAELVQNKSILRGLINAADDIAELAYSDPENIEEAMDVAEKKVYQ
                     VTNAPTTQKFTPIGSSLTEAWDRFEKLNSSEDAHRGIPTGFSAIDNLLAGLQKSDLII
                     LAARPSMGKTTFALDIARNAALLAGASVGIFSLEMSDQQLVDRLLAAEAGVDSWKLRT
                     GKLSSDHEFEAVQAAMARLAEAPIHIDDQPGNNILKMRSSARRLKNEHGLDLLIVDYL
                     QLMSPTATKASDSMVQQVTEISRSLKILARELDVPVVALSQLSRAVEQRGGKPRLSDL
                     RDSGSIEQDADVVMFIHREDKINKESDRPNIAEILIEKHRNGPVGSAELYFDEKHVRF
                     INIDTHHTMGAAAGDF"
     gene            2357..2533
                     /locus_tag="VFS75_00210"
     CDS             2357..2533
                     /locus_tag="VFS75_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677125.1"
                     /translation="MANDFVCARCGRTRADHETDTESSNCAGYEPSANEREAADIETR
                     QVFSEIKASTVWIT"
     gene            complement(2574..4028)
                     /gene="cysS"
                     /locus_tag="VFS75_00215"
     CDS             complement(2574..4028)
                     /gene="cysS"
                     /locus_tag="VFS75_00215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU4677126.1"
                     /translation="MGFLDFFVSKRADEVPMYFVNSATGKKERFVPRKAGHLSMYSCG
                     PTVYDHIHIGNLRAYLLPDLVRRVATYNGYEVKNVINFTDFGHLTDDADAGEDKMMKA
                     LRREGKPITLTAMREVAETYMRSFKDDNAAFRNLPPTYYTPASDYIREEIKLVETLVE
                     KGYAYETSDGIYFDISKFPSYGVLGNVNVSEQEAGARVAENPEKRHPADFALWKKGLL
                     GWESAWGKGFPGWHVECTAMVFAKLGKQIDVHTGGEDLKYTHHNGEIAEAEATTGKSY
                     VSYWLHNAHLKMDGTKIAKSLGNGLRLQNLVDRGYSPLVYRYWLLTGHYRTPMNFTFE
                     ALDASKQALFRLKRFMFEEWKNESGTIDEAYRRRFHEAINDDLDTPKAIAIMWELVKD
                     GNVKPADKVATIREMDTIFDVGLRDEASDVSRELGIVGADELPDDVRTLLDERELARA
                     ARNWPESDRIREALNLKGYVVEDGPQGQKVSKST"
     gene            complement(4068..4979)
                     /locus_tag="VFS75_00220"
     CDS             complement(4068..4979)
                     /locus_tag="VFS75_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU4677127.1"
                     /translation="MTERFAQGKLRWINMKRPTPAEIHKIMLELELPPVLMGDLSSAV
                     PKNSVCRADDTLKLTLDFPAVKNISPEHQLEVKFFVTRDTLLTVHYEEMEGIDRFKRQ
                     LELTSTLRKRQVHLSGAHLFIALFNNLYDATSSKLDYLESKLSDIETEIFYDNEKKLV
                     FDIAQISKVLISFRHVMRSHEDILHDLTPLFTDLFGSSFKRDLENLGDHYDSLARRLA
                     TVYETMTALRETNIAMLETKQNEIMKIFTILAFTTFPLTLLSSLFGMNTDSTPIVGLR
                     GDFWIIIGVMVLATISFFVYFRHKGWM"
     gene            5059..6327
                     /locus_tag="VFS75_00225"
     CDS             5059..6327
                     /locus_tag="VFS75_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU4677128.1"
                     /translation="MQDDITTHEDERDDRDDAPRRPTLLGVGLAVLLATATFFSGIHI
                     GSDTKLEANVFSFLMPQTAADTSVDMSEFWRVWNLLNEKFVTTTSTPVTNGEKIEGAI
                     EGLVRSYGDPYTVYMPPEEANSFNEDISGNFSGVGMEVGIRDDVLTVISPLPQSPAEQ
                     AGIMAGDMILRIDGESTESMSVDEAVKRIRGEVGTEVNLTIFRKGESEYREFTVKRDN
                     IVIPTSKTEIRGDVFIVTLYSFNAISESEMQNALRAYVRSGKKNLVLDLRGNPGGYLE
                     SAVSIASYFLPVGKVVVRENFGDGQDEQLYRSTGKELRHFTPDDFVVLVDEGSASASE
                     ILAGALKEHGVATLIGAKTFGKGSVQELVKLDNGANLKVTVARWLTPNGEWITGNGIE
                     PDITVERSADDRDAGKDPQLDAALDFLKKH"
     gene            6405..6845
                     /gene="rplI"
                     /locus_tag="VFS75_00230"
     CDS             6405..6845
                     /gene="rplI"
                     /locus_tag="VFS75_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU4677129.1"
                     /translation="MKVILIRDVARLGRKSEVKDVPSGHAQNFLIPRKLAIPATPENV
                     RRLGEAMKKNAAAEAERMETFRAALAACAAKPVTIEAEANEQGHLFKGLHAKDVVHAL
                     AAAGCEFDESDIVLSAPIKSIGTHTVALERGDEKGSATLEVVKK"
     gene            7032..7553
                     /locus_tag="VFS75_00235"
     CDS             7032..7553
                     /locus_tag="VFS75_00235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015214299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4677130.1"
                     /translation="MNPVAIMKTSEGTIKLELFEDKMPITAANFIKLAKDGFYNGVKF
                     HRVIDGFMIQSGDPNTKGDDPSTYGTGGPGYTIQDEFVSDPLLSNVRGTIAMANTGQP
                     NSGGSQFFINTVDNTGLDFDKQPFTSKHPVFGRVIEGMDVVDKISKVETDGRDMPKTP
                     VVIESVTIVEKGA"
     gene            complement(7595..7873)
                     /gene="rpmA"
                     /locus_tag="VFS75_00240"
     CDS             complement(7595..7873)
                     /gene="rpmA"
                     /locus_tag="VFS75_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU4677131.1"
                     /translation="MSTKKAGGSAKNLTDSQAQYLGVKRADGEKVNAGEVIVRQRGTK
                     IEAGKNVAVGKDHTLFAMKAGIVKFGRVRKTNFTGKTTTKTKVGVEAA"
     gene            7972..8298
                     /locus_tag="VFS75_00245"
     CDS             7972..8298
                     /locus_tag="VFS75_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677132.1"
                     /translation="MSRDILLSALIVGVSVASFALGRLSAGQRTVPRPVLETAAVPDA
                     LATDTQREEAAVEGGYVASKTGTKYHLPWCPGASQIKESNKIWFTTKEEAEAAGYAPA
                     ANCKGI"
     gene            complement(8295..8681)
                     /locus_tag="VFS75_00250"
     CDS             complement(8295..8681)
                     /locus_tag="VFS75_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4677133.1"
                     /translation="MSTKTKILVVDDEKDLREALASALTDAGYDVSAAEDGKSGLDAA
                     RAQKPDLILLDIMMPKMNGHQVLHELRRDAWGKTVPVLLLTNADDAGNIVTGIGEKSD
                     EYLIKSQTSLKDIVTRVKQYLAGYHN"
     gene            complement(8694..9362)
                     /gene="ung"
                     /locus_tag="VFS75_00255"
     CDS             complement(8694..9362)
                     /gene="ung"
                     /locus_tag="VFS75_00255"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4677134.1"
                     /translation="MDVSIEPSWKEALKEEFDAPYFRELSDTVRNAYLANSAVYPPPR
                     DVFRAFELCPFERVRVVILGQDPYHGAGQAHGLSFSVRDGMGLPPSLQNVYKELHDDL
                     GVPMPTSGDLTRWAKQGVLLLNATLTVEGGKPGSHQGLGWERFTDAVIRKLSSDREHL
                     VFILWGKYAQEKGAHIDGSKHLVLRSPHPSPFSARSGFFGSKPFSKTNAYLREHGESP
                     IDWS"
     gene            9418..10014
                     /locus_tag="VFS75_00260"
     CDS             9418..10014
                     /locus_tag="VFS75_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEU4677135.1"
                     /translation="MIVIDTEASGTNYDKNSIVSLAAIDFSDPDNRFYEECRIWDGAH
                     INDESLAITGFSVEEVTDPKKKPEGEVVRMFLEWSQHMADRTLAGQNVSFDRDFLKAA
                     SEREGLSWDLAHRTIDTHTLCYMHMVTHGVTPPIDPEHRRSALNLDAVLNYCGIPDEP
                     EPHNALTGALCHAEVISRLLYGKKLLSEFDAYDIPWTT"
     gene            10080..10910
                     /locus_tag="VFS75_00265"
     CDS             10080..10910
                     /locus_tag="VFS75_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU4677136.1"
                     /translation="MATTKKTSPVKKFRLGLLGKRDPAAEIRVSIPVKSDPTTKKFKL
                     KRDGRDMHCRVVTDLFEQDDAIQSWRIFRIMSEFVNGFDVLRKYSTAATFFGSARLDP
                     TDPMYKAAEQLAADLAKKGFTIITGGGPGIMEAANVGAFKVGGKSIGLNIKLPAEQKL
                     NPYVSESESFNYFFSRKVMLSFASEIYIYFPGGFGTLDEFFEIVTLVQTKKISRIPIV
                     LYGKEFWEPLIEFFEKRLYKEYKTIAKEDLELFHVVDSVDEAYDYVMKHVDLVAPRQI
                     "
     gene            10919..12181
                     /locus_tag="VFS75_00270"
     CDS             10919..12181
                     /locus_tag="VFS75_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677137.1"
                     /translation="MKTLFKHSALSQTRLAAFEERLVRYTDGLVEGFDATLSYGRPES
                     ALLLPGDRAYQAELDRAVSRLTPVDHVVLIGIGGSSLGALAVYHALRSETTPRFTVID
                     ELDREALLRLETLLAECKNPSKLAVIVVSKSGTTTETMLNAVKAIAAGERRFGDAFHH
                     RLVFISDKGTKFLDAGRRKGALCLTIPSAVGGRFSVLSAAGIVPLSLLGFDVRSLRRG
                     ARDALSRVPLARTQKAASILALHALEGYRAVNFFTFNRRFAHLGYWYRQLLAESIGKR
                     MTVRGATFSHQLLPIVSTGADLHSTAQLFLSGYAGMYTHFVYYRDVRAYRALRSHWLL
                     SHVPFLKGKSVGEVSEAIVKGVLRSYSKEHLPYRATELEGATAYEVGLLMNSLMAEVM
                     CLAHLLSVDAFDQPNVESYKAATRKFLA"
     gene            12186..>12853
                     /locus_tag="VFS75_00275"
     CDS             12186..>12853
                     /locus_tag="VFS75_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4677138.1"
                     /translation="MFIHFDIGGTKTRVAASRDLASLERVAKFDTPRTYREGLEALLK
                     EMREVAGGEPIEGVSGGIRGPLNHEKTGIISETELADWVGRNLVLDLRSALDAPVYVE
                     NDTAMVGLGEVHFGAGKGYRIVAYHTVSTGVGGARFVDGRLDVTSVGFEPGHQVLDID
                     QTILGRHVPPTLQNLVSGRALEKRRGVKPYEIGQEDPVWDELARYLASGLKNTIVYWS
                     PDAIV"
BASE COUNT         2455 a         3817 c         4036 g         2545 t
ORIGIN      
        1 tcctcgtcga cgacggcaag gatgcgacga tcggcatgcc gtacatcaag ggcgcgacgg
       61 tgaaggggac cttcgaaggc gtgaagaagg ggaagaagat caccatcgtt cgctacaagg
      121 cgaaatcgaa ccgcgaccgc aagatcggtc accggcagaa gcacgccgcg gtgcgtatcg
      181 aggcaatcta aatacgaaaa aaccggcagg agccggtctt ttcattcgcg atgcttgagg
      241 gagttcttga tggtctccgg atcggcgtac tcgagctcgg agcccgtcga gagacctcgt
      301 ccgaggtgcg agagcttaat ggggttcgcg gcgaggagct cgtgaaggag cgattcgacg
      361 taccggccgg tgttctcgcc gtccgggttc accgagaagc cgagaatgac ctcggagagg
      421 ccctccttcg cacgctcacc gaccgtgtgc ttcaaggcgc cgccgcggag ccccttcacc
      481 tcctcggtgc cgaggagggg gacggtaccg cctaagacga agtagaggcc gtcatagacg
      541 ccgcttcgct ctatcgcgtc gatgtcggta tctcgttcga cgacgaggag cttcgaacgg
      601 tcgcgaaccg cgctctcgca gagcgcacag cgctcgctcg cgcgtcccga gtggaagcgg
      661 tggcatgacg ggcactcggt gacctcgtcc ttgatggtgc ggatgagctc ggcgaactca
      721 cgaaggtcgc tctctcccat ggtgaggaga tggaacacga atcggcgcgc ttggcgtgag
      781 ccgataccgg ggaacttctc gaagtagcgg gcgagtttgt cgaagggcgt catgagagtt
      841 tagaaatcgc ccgccgcggc gcccatcgtg tggtgcgtat cgatatttat gaagcggacg
      901 tgcttctcgt cgaagtagag ctcggccgaa ccgacggggc cgtttcggtg cttctcgatg
      961 agtatctcgg cgatgttcgg tcggtcggac tccttgttta tcttgtcttc gcggtgaatg
     1021 aacatgacga cgtcggcgtc ctgttcgatc gagcccgagt cgcggaggtc ggagaggcgc
     1081 ggcttcccgc cacgctgttc gaccgcgcgc gagagctggg agagcgcgac gacggggacg
     1141 tcgagctcgc gggcgaggat cttcaaggaa cgcgatattt ccgtgacctg ctggaccatc
     1201 gagtcggatg ccttcgtcgc ggtcgggctc atgagctgga ggtagtcgac gatgaggagg
     1261 tcgaggccgt gctcgttctt gagtcggcgc gccgaagagc gcattttcaa aatattgttg
     1321 cccggctggt cgtcgatgtg gatgggcgcc tctgcgaggc gtgccatcgc cgcctggacc
     1381 gcctcgaact cgtggtcgga ggagagcttt cccgtgcgga gtttccacga atcgacgcca
     1441 gcctccgcgg cgagaaggcg gtcgacgagc tgttggtcgc tcatctcaag cgagaagatg
     1501 ccgaccgacg cgcccgcgag gagcgcggcg ttacgcgcga tgtcgagcgc gaacgtcgtc
     1561 ttacccatcg agggacgcgc ggcgaggatg atgagatcgg acttttggag gccggcgagg
     1621 aggttgtcga tcgcgctaaa gccggtcggt atgcctcggt gcgcgtcctc ggacgaattg
     1681 agcttctcga agcggtccca cgcctctgtg agcgagctac cgatgggcgt gaacttctgc
     1741 gtcgtcggcg cgttcgtcac ctggtacacc ttcttttcgg cgacgtccat cgcctcttcg
     1801 atgttctcgg ggtcgctgta cgcgagttcg gcgatgtcgt cggccgcatt gatgagcccg
     1861 cggaggatgg atttgttctg gaccaattcc gcgtagtaga tcgcgtttcc ggccgcgggc
     1921 acggattcga cgagctccgt cacgtagctc gccccgccga cgcgctcgag gaggttccgc
     1981 gtcttcagtt tcgtcgtcac cgagagaagg tcgatcgggt cgccatgcat gaacgtatcg
     2041 aggatggcgc ggtagatctc gcggtgtttg tccgcgtaga acgattcggg gaagacggtc
     2101 accgaaacgt cgtgcatcgc ctccggcttc agcatgatgg cgccgaggag cgcgcgctcc
     2161 gcatcgaggt tctgcggcgg cgtccggagg agtctgctct tcgtcgcggt ttgcttcacg
     2221 tcttcgcgtg tctcatccat ggtgaatgca ttgtagcacg ggggtaaaaa cgggcaggga
     2281 agggacgttt ggggatgtcc tggggatacc gatttgtggt atagtgaagc cgttctttgg
     2341 acacggcgga ggccacatgg caaacgactt cgtttgcgcg cgatgcggac ggacgagagc
     2401 ggatcacgag accgatacgg aatcatcgaa ctgcgccggc tacgagcctt ccgcgaacga
     2461 gcgcgaagct gccgacatcg agacgcggca ggtgttctcg gagatcaagg cgtcgacggt
     2521 ctggatcacg tgagcgtaag cgaaagggca ccctatgggt gcccgttttt ttcttacgta
     2581 ctcttcgaga ccttctgccc ttgcggcccg tcttcgacga cgtacccttt gagattgagc
     2641 gcctcgcgaa tgcgatcgga ctccggccag ttacgcgcgg ctcgggcgag ctcgcgctcg
     2701 tcgaggagcg ttcggacgtc atcggggagc tcatccgccc cgacgatgcc gagctcgcgc
     2761 gagacgtcgc tcgcctcgtc gcggaggccg acgtcgaaga tggtgtccat ctcgcggatg
     2821 gtcgcgacct tgtccgcagg cttcacgttc ccgtccttca cgagctccca catgatggcg
     2881 atcgccttcg gcgtgtcgag gtcgtcgttt atcgcttcgt gaaagcgcct ccggtacgcc
     2941 tcgtcgatcg tgcccgattc gttcttccat tcttcgaaca tgaagcgttt gagccggaag
     3001 agcgcctgct tcgacgcgtc gagcgcttcg aacgtgaagt tcatcggcgt ccggtagtga
     3061 ccggtgagaa gccagtagcg atagacgagg ggagagtacc cgcggtcgac gaggttctgg
     3121 agtcggaggc cgtttcccag ggacttcgcg atcttcgtgc cgtccatctt gaggtgggcg
     3181 ttgtggagcc agtacgagac gtacgatttt ccggtcgtcg cttccgcctc tgcgatctcg
     3241 ccgttgtggt gcgtgtactt gagatcctca ccacccgtgt ggacgtctat ctgctttccc
     3301 aatttcgcga agaccatcgc ggtgcactcg acgtgccacc cggggaagcc ctttccccac
     3361 gcgctctccc aaccgaggag gcctttcttc cagagcgcga agtccgcggg atggcgcttc
     3421 tcgggattct ccgcgacgcg tgcacccgcc tcctgttcgc tcacgttcac gttcccgagt
     3481 acgccgtacg aggggaattt cgagatgtcg aagtagatgc cgtccgaggt ctcgtacgcg
     3541 taccccttct cgaccaaggt ctcgacgagc ttgatctcct cgcggatgta gtcgctcgcg
     3601 ggcgtgtagt acgtcggcgg taggtttcga aacgcggcgt tgtcgtcctt gaacgaccgc
     3661 atatacgtct ctgctacctc acgcatcgcg gtcagcgtaa tcggtttccc ctcgcggcgg
     3721 agcgccttca tcatcttgtc ctcgcccgcg tcggcgtcat cggtgaggtg gccgaagtcg
     3781 gtgaagttga tgacgttctt cacctcgtac ccgttgtacg tcgcaacgcg gcgaacgagg
     3841 tcggggagga ggtatgcgcg gaggttcccg atgtggatgt ggtcgtacac cgtcgggccg
     3901 catgagtaca tggagaggtg tccggccttt cgcggaacga agcgctcttt cttcccggtc
     3961 gcggagttca caaagtacat aggcacctcg tccgcgcgct tcgaaacgaa aaaatcgagg
     4021 aatcccatag ataaattcag tgtagcaaat accgcggatt cgtcatctca catccagcct
     4081 ttgtgcctga agtacacgaa gaagcttatg gtcgcgagga ccatcacgcc gatgatgatc
     4141 caaaagtcgc cgcgaagacc gacgatgggg gtcgaatcgg tgttcatgcc gaagagcgaa
     4201 ctgaggagcg tgagcgggaa cgtcgtgaac gcgagaatcg tgaatatctt catgatctcg
     4261 ttctgcttcg tctcgagcat cgcgatgttc gtctcacgga gcgcggtcat cgtctcgtag
     4321 accgtcgcga gccgccgcgc gagcgaatcg tagtggtcgc cgaggttctc caagtcgcgc
     4381 ttgaaggacg aaccgaagag gtcggtaaag agcggcgtga ggtcgtggag gatgtcctca
     4441 tggctccgca tgacgtgtcg gaaggagatg aggaccttgc ttatctgcgc tatgtcgaag
     4501 acgagcttct tctcgttgtc gtagaagatc tccgtctcga tgtccgagag cttcgactcg
     4561 aggtagtcta gttttgacga ggtagcatcg tagaggttat tgaagagcgc gatgaacagg
     4621 tgcgcgccag agagatgcac ttgtcgcttc cggagcgtcg aggtgagctc gagctggcgc
     4681 ttgaagcggt cgatgccctc catctcctca tagtggaccg tgaggagcgt atccctcgtc
     4741 acgaagaact ttacctcgag ctggtgctcg ggggagatgt tcttcaccgc cgggaaatcg
     4801 agggtcagct tgagcgtgtc gtcggcgcgg cacaccgaat tcttcggaac ggcgctcgaa
     4861 aggtccccca tgaggacggg tgggagctcg agctcgagca tgatcttgtg gatctcggcc
     4921 ggcgtaggcc gcttcatatt gatccagcgc agtttccctt gcgcgaagcg ttccgtcatg
     4981 tccctatttt atcacgcacg tccgtcgcca acggttcttt tttgtataac tgggggagag
     5041 tgtgcgataa tctctctcat gcaggacgac atcaccacgc acgaagacga gcgagatgac
     5101 cgagacgacg caccgcgcag accgacccta ctcggggtcg ggctcgccgt ccttctcgcg
     5161 accgcgacgt tcttctcggg tatccatatc ggttccgaca cgaaactgga ggcgaacgtc
     5221 ttctcgttcc tgatgccaca gaccgcggcc gacacgtcgg tcgacatgag cgagttctgg
     5281 cgggtatgga atctcctcaa cgagaagttc gtcacgacca cctcgacgcc ggtcacgaac
     5341 ggcgagaaga ttgagggcgc catcgagggc ttggtgcggt cgtacggtga cccgtacacg
     5401 gtctacatgc cgcccgagga ggcgaactcc ttcaacgagg acataagcgg caacttcagc
     5461 ggcgtcggca tggaggtcgg cattcgcgat gacgtgctca ccgtcatctc gcccctcccc
     5521 cagagtccgg cggagcaggc gggcatcatg gccggcgaca tgatactccg catcgatggc
     5581 gagtcgaccg agagcatgag cgtcgacgag gcggtgaagc gcattcgcgg cgaagtcggc
     5641 accgaggtca acctgaccat cttccggaag ggcgaatcgg agtaccgcga gttcaccgtg
     5701 aagcgcgaca acatcgtcat cccgacgagc aagacggaga ttcggggcga cgtcttcatc
     5761 gtgacgctct acagtttcaa cgcgatatcg gagagcgaga tgcagaacgc gctccgtgcg
     5821 tacgtgcgaa gcggtaagaa gaatctcgtc ctcgacctgc gcgggaatcc gggcgggtat
     5881 ctcgagagcg cggtctcgat cgcgagctac ttcctcccgg tcggtaaggt cgtcgtccgc
     5941 gagaacttcg gtgatggaca ggacgaacag ctctaccgaa gcacggggaa ggaactgcgc
     6001 cacttcacgc ccgacgactt cgtcgtcctc gtcgatgagg gctccgcgtc cgcctcggag
     6061 atccttgccg gcgccctcaa ggaacatggc gtcgcgacct tgatcggcgc gaagaccttc
     6121 ggaaagggct cggtccagga gctcgtgaag ctcgacaacg gggcgaacct caaggtgacc
     6181 gtcgcgcgct ggcttacccc gaacggtgag tggatcacgg ggaacggcat tgaaccggac
     6241 atcaccgtcg aacgctcggc ggacgaccga gacgcgggga aggaccccca gctcgacgcc
     6301 gctctcgact tcctcaagaa acactgaaag ccggagaatc cccctcgttt gaatcacgag
     6361 ggggatttgc tatgatgggt gtctcttacc aataacccac gaacatgaag gtcattctga
     6421 tacgagacgt cgcgcgcctc ggacgcaaat ccgaggtgaa ggacgtgccg agcgggcacg
     6481 cgcagaactt tctcattccg cgaaaactcg cgattcccgc gacgccggag aacgtccgcc
     6541 gtctcggcga ggcgatgaag aagaacgcgg cggcggaggc ggagcgcatg gagacgttta
     6601 gggcggcgct cgcggcgtgc gcggcgaagc cggttacgat agaggccgag gcgaacgagc
     6661 aggggcatct cttcaaaggg ctccatgcga aggacgtcgt ccacgcgctc gcggccgcgg
     6721 gctgcgagtt cgatgagagc gacatcgtcc tctcggctcc catcaaatcg atcggcacgc
     6781 atacggtcgc gctcgagcgc ggcgacgaga aggggagcgc gacgttagaa gtcgtaaaga
     6841 aataaggaat ggcaagcatc aaacagatcg cgttcgtggt cggcctctcg gtcgtcctcg
     6901 tcgcggcgat aggctggtgg gtcaatcgag cgaacgaggc gccagaactt ctcctacgcg
     6961 agaatcagaa gctcgacgcg ccgctctcgc cgcaggaatt aaaaacgaat aatccaggca
     7021 tcacgaacac catgaatcca gtagccatca tgaagacgtc ggaagggacc atcaagctcg
     7081 agctcttcga ggacaaaatg cccatcaccg cggcgaactt catcaaactc gcgaaagacg
     7141 gcttctataa cggcgtgaag ttccatcgcg tcatcgacgg gttcatgatc cagtcgggcg
     7201 acccgaacac gaagggagac gacccctcga cgtacgggac cggcggtccc gggtacacga
     7261 tacaagacga gttcgtctcg gacccgcttc tctccaatgt tcggggcacc atcgcgatgg
     7321 cgaacacggg ccaaccgaac tcgggcggga gccaattctt catcaatacg gtcgacaaca
     7381 ccggtctcga cttcgacaag cagccgttta cctcgaagca cccggtcttc ggacgcgtca
     7441 tcgagggcat ggacgtcgtc gacaagatct cgaaggtcga gacggacggg cgagacatgc
     7501 cgaagacgcc ggtcgtcatc gagtcggtga cgatagtgga gaagggggcg taggcaaaag
     7561 aaaacagcgt ctcgcgacgc tgttttcttt tgccttaggc ggcctcgacg ccgaccttgg
     7621 tcttcgtggt cgtctttcca gtgaaattgg tctttcggac gcgcccaaac ttcacgatgc
     7681 ccgccttcat ggcgaagagc gtgtggtcct tgccgaccgc gacgttcttg ccggcttcga
     7741 tcttcgtgcc gcgctgtcga acgatgacct cgcccgcgtt gaccttttcg ccgtccgcgc
     7801 gctttacgcc gaggtactgc gcctgcgagt cggtgaggtt cttcgcgctg ccgcctgctt
     7861 ttttggttga catacgatta tttctgtgga ccgggggagg aggcctcgcc gcggtgtata
     7921 atgaaccggg tgagtataca agaattatcc tcaaagttca agggtttttt catgtcgcga
     7981 gacatcctcc tttcggcgct catcgtcggc gtttcggtgg cctcctttgc cctcggcaga
     8041 ctttctgcgg ggcagcggac ggtcccccga ccggtactcg aaacggccgc cgttcctgat
     8101 gcgttagcaa cggataccca acgcgaagag gcggccgtcg agggcgggta cgtcgcctcg
     8161 aaaacgggga cgaaatacca cctcccatgg tgtccgggcg cgtcgcagat caaagaatcg
     8221 aacaagatct ggttcacgac gaaggaggag gcagaggcgg cgggatacgc gcctgcggcg
     8281 aattgtaagg gaatctagtt gtggtatccg gcgaggtact gcttcacgcg cgtcacgatg
     8341 tccttgaggc tcgtctgcga tttgatgagg tactcgtcgc tcttctctcc gatgcccgtc
     8401 acgatgttgc cggcgtcgtc cgcgttcgtg aggagaagga cgggaacggt cttaccccat
     8461 gcgtcccggc gcagttcgtg gaggacctga tgtccgttca tcttcggcat catgatgtcg
     8521 aggaggatga ggtcgggttt ctgcgcgcgc gccgcgtcga gacccgattt gccgtcttcc
     8581 gcggcgctca cgtcgtaccc cgcgtccgtg agcgcggacg cgagcgcttc gcggaggtcc
     8641 ttttcgtcgt cgacgacaag tatcttcgtc ttagtgctca ttgccgcatt gtatcacgac
     8701 cagtcgatgg gggactcgcc gtgctcccta aggtacgcgt ttgtcttact gaagggtttc
     8761 gagccgaaga atccgcttcg agccgagaag ggggaagggt ggggagaacg gaggacgagg
     8821 tgcttcgagc cgtcgatgtg cgcccccttc tcttgcgcgt acttccccca gaggatgaag
     8881 acgaggtgct cgcggtcgct cgagagtttt cggatgaccg cgtcggtgaa acgctcccac
     8941 ccgagacctt ggtgcgagcc gggtttgccg ccttcgaccg taagcgtcgc attgagaagg
     9001 agaacgccct gcttcgccca ccgggtaagg tctccggagg tcggcatggg aacgccgagg
     9061 tcgtcatgga gctctttgta gacgttttgg agagacggcg ggagacccat gccgtctctg
     9121 accgagaagg aaagtccgtg tgcctgtccc gcaccgtggt atgggtcttg tccgagtatg
     9181 acgacgcgga ccctctcgaa ggggcagagc tcgaaggcac ggaatacatc tcggggcggc
     9241 ggatacacgg cggaattggc gaggtacgca tttcggaccg tgtcgctcag ttccctgaag
     9301 tacggcgcgt cgaactcctc tttgagcgct tccttccacg acggttcgat ggagacgtcc
     9361 atggtccgat tgtagcaaac ggccggcagg tcgcgagcgc cgtggtatcc tagtcccatg
     9421 atagtcatag acaccgaagc ctcggggacg aactacgata agaactcgat cgtctccctc
     9481 gccgcgattg atttcagcga tccggacaac cgattctacg aagagtgccg catctgggac
     9541 ggcgcgcaca taaacgacga gtcgctcgcc atcacgggat tcagcgtcga ggaggtcacc
     9601 gatccgaaga agaagcccga gggcgaggtc gttcgcatgt tcctcgagtg gtcgcagcat
     9661 atggccgacc gaacgctcgc gggacagaac gtctccttcg accgcgactt cctgaaggcc
     9721 gcctcggagc gcgagggtct ctcgtgggac ctcgcccacc gcaccatcga cacgcacacg
     9781 ctctgctaca tgcacatggt cacccatggc gtgacgccgc ccatcgaccc ggagcaccgt
     9841 cgctccgcgc tcaacctcga cgcggttctc aattactgcg gcatccccga cgagccggaa
     9901 ccccataacg cgctcaccgg tgcgctctgt catgccgagg tcatttcccg cctcctctac
     9961 ggcaaaaaat tgctttccga gttcgacgcg tacgacatac cgtggacgac gtgacctcac
    10021 gtccactttt tcactgcata tctcgcgttt ctcgaccacg cgcgtgataa aatgtgctca
    10081 tggcaaccac aaaaaagacc tctccggtca agaaatttcg gcttgggctc ctcgggaagc
    10141 gtgatcccgc ggccgagata cgagtctcca tccccgtgaa aagcgacccg acgacgaaga
    10201 agttcaaatt gaaacgcgac ggccgagata tgcattgtcg agtcgtcacc gatctattcg
    10261 agcaggacga tgccattcag tcatggcgca tcttccgcat catgtcggag ttcgtgaacg
    10321 gattcgacgt cctccggaag tactcgaccg cggcgacctt cttcggaagc gcgcgtctcg
    10381 accccaccga tcccatgtac aaagccgcgg aacagctcgc cgcggacctc gcgaagaaag
    10441 gcttcaccat catcaccggc gggggacccg gcatcatgga ggcggcgaac gtcggcgcct
    10501 ttaaggtcgg cggtaagtcg atcgggctca acatcaaact ccccgcggaa cagaaattga
    10561 atccctatgt gtccgaatcg gagagcttca actacttctt ctcgcggaag gtcatgctct
    10621 cgttcgcgtc cgagatctat atctacttcc cgggcggctt cggcacgctc gatgagttct
    10681 tcgagatcgt gacgttggtc caaacgaaga agatatctcg catccccatc gttctctacg
    10741 ggaaggagtt ttgggaaccg ctcatcgaat tcttcgagaa gcggctctac aaggagtaca
    10801 aaacgatcgc gaaggaggac ctcgagctct tccacgtcgt cgactccgtc gatgaggcgt
    10861 acgactacgt catgaagcac gtcgacctcg tcgcgccgcg acagatctaa cgaccgctat
    10921 gaagaccctc ttcaaacaca gcgctttgtc gcagacgcgt ctcgccgcgt tcgaagagag
    10981 gctcgtgcgc tataccgatg gcctcgtcga gggattcgat gcgaccctct cctacgggcg
    11041 gcccgagtcg gcgctcctcc ttccgggtga tcgggcgtat caggcggagc tcgaccgcgc
    11101 cgtctctcga cttactcccg tcgaccacgt cgttctcatc ggcatcggtg gttcaagcct
    11161 cggcgctctc gccgtgtacc atgcgctccg gagcgagacg acgcctcgct tcaccgtcat
    11221 tgacgagctc gaccgagagg cgctccttcg tcttgagacg ctccttgcgg aatgtaagaa
    11281 cccctccaag ctcgcggtca tcgtcgtgag caagtccgga acgacgaccg agacgatgct
    11341 caacgcggtg aaggcgatcg cggccggcga acgccggttc ggcgatgcgt ttcatcatcg
    11401 gctcgtgttc ataagcgata agggcacgaa gttcctcgat gcggggcgga gaaagggagc
    11461 gctctgcctc accatcccgt cggctgtcgg cggccgtttt tccgttctct cggccgccgg
    11521 catcgtgccg ctttcgctcc tcgggttcga cgtacgctcg ctccgccgtg gggcacggga
    11581 cgcgctctcg cgcgtcccgc tcgcgcgtac gcagaaggcg gcgtccattc tcgcgctcca
    11641 tgcgcttgag gggtaccgtg cggtcaattt tttcacgttc aatcgccggt tcgcgcacct
    11701 cggctactgg tatcgacagc tcctcgccga gagcatcggc aagcggatga cggtgcgagg
    11761 tgctacgttc tcgcaccagc ttttgcccat cgtctcaacg ggcgcggacc ttcactcgac
    11821 cgcccaactt ttcctctcgg ggtatgcggg catgtacacg cacttcgtct actaccgcga
    11881 cgtccgcgcg tatcgggcgc tccgctccca ttggctcctc tcgcacgtac cgtttctcaa
    11941 gggtaagagc gtcggcgagg tgagcgaggc catcgtgaag ggcgtcctac gatcgtactc
    12001 gaaggagcac cttccatacc gtgcgaccga gctcgaaggt gcgaccgcgt acgaggtcgg
    12061 gcttctcatg aactcgctca tggcggaggt catgtgtctc gcgcacctcc ttagcgtcga
    12121 cgcgttcgat cagccgaacg tcgaatccta caaggccgcg acccgaaagt tcctcgcgta
    12181 gcaccatgtt catccacttc gacatagggg gtacgaaaac gcgcgtcgcc gcgtcgcgcg
    12241 acctcgcgtc cctcgagcgc gtcgcgaagt tcgatacgcc ccgcacgtac cgcgaggggc
    12301 tcgaagcgct cctaaaagag atgcgcgagg tggcgggagg cgaacccatc gagggcgtgt
    12361 caggaggcat ccgcggaccg ctcaaccatg agaagacggg catcatcagc gagaccgagc
    12421 tcgccgattg ggtaggacgg aacctcgtcc tcgacctccg tagcgcgctc gatgcgcccg
    12481 tgtacgtcga gaacgatacc gcgatggtcg ggctcggcga ggtccatttc ggggcgggga
    12541 aggggtatcg tatcgtcgcg taccacaccg tctcgacggg tgtgggcgga gcccggttcg
    12601 tcgatggacg gctcgacgta acgagcgtcg gattcgagcc gggacaccag gtcctcgaca
    12661 tcgaccagac gattctcgga cgccacgttc cgccgaccct ccaaaacctc gtatccggcc
    12721 gcgccctcga gaagcgtcga ggagtaaagc cgtacgagat cggccaggag gacccggtgt
    12781 gggacgagct cgcgcgatat ctcgcgtctg ggctcaaaaa caccatcgta tattggtcgc
    12841 cggacgccat cgt
//
LOCUS       DASTSP010000008         6500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_915788, whole genome shotgun sequence.
ACCESSION   DASTSP010000008 DASTSP010000000
VERSION     DASTSP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915788"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..652
                     /locus_tag="VFS75_00280"
     CDS             389..652
                     /locus_tag="VFS75_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677139.1"
                     /translation="MRIRMLESFGKPEMSPLETYVEEFKKDYEELTRLAAASAEHAAG
                     LGYGTDRDEKAKEMRERFEAMLEDVLGPKKEGEGDAQSELTLQ"
     gene            complement(700..933)
                     /locus_tag="VFS75_00285"
     CDS             complement(700..933)
                     /locus_tag="VFS75_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677140.1"
                     /translation="MEMVAAWLHEPSVYFTLAAVVLIVILTGVLRKHLRSTRYERHAK
                     NRRKAEARALEKQHIDSYIGGEALTSHAFHNGD"
     gene            1177..1572
                     /gene="mscL"
                     /locus_tag="VFS75_00290"
     CDS             1177..1572
                     /gene="mscL"
                     /locus_tag="VFS75_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU4677141.1"
                     /translation="MRKFFNEFKQFAMRGNVLDLAIAVVIGGAFNKIVSSLVDTIITP
                     AIGLLIGGVDFSALSFTVGASTFKYGLFVQNVVDFVLIALIIFLVVKAMNRFHRREEE
                     EEEKLPPEPPEEVKLLREIRDELKNGSRS"
     gene            1864..4788
                     /locus_tag="VFS75_00295"
     CDS             1864..4788
                     /locus_tag="VFS75_00295"
                     /inference="COORDINATES: protein motif:HMM:NF005544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEU4677142.1"
                     /translation="MGDYQRKQNRFMITTIIKRNGTTVPFDPTKIVVVMKKAFAAEQV
                     PIDDAALEQMTGRVVANLTTMCDGKENCPTVEQVQDLVENTLMERGYFTVAKAYILYR
                     YKQSEERKQEVIKEIEEQRLTVRRSDGSVEQFSREKMRAYLAEFVYGYEKEIDLDELV
                     SQLESEVYDGIKTSEIAKLATLVLRSRIEQDPAYSIVASRQLARRIADEAIGNTRSLS
                     AEERTAAYRNAFVRNIKLGVEAKKYDAGLLSFDLEKVAAAIEPSRDDLFRYLGMETLH
                     DRYFVRLDEKRVIETAQMMWMRIAMGLALNEKDKEGRAVEFYNVMSRLRFVPSTPTLF
                     HAGTPHPQLSSCYLNVVEDDLKHIFKVYGDNAMLSKWAGGIGTAWSKLRGTGAVLKSS
                     GITSQGVIPFLKIANDVTVAINRSGRRRGATCVYLENWHIDFEDFLELRKNTGDERRR
                     THDMNTAAWVSDLFMKRVKEDGDWTLFSPDETPDLTETYGKQFEEAYEKYEEMADRGE
                     LRLFKRIKARDLWKKHLSMLFETGHPWVTFKDPSNVRSPQDHVGVVHNSNLCTEITLN
                     TSAEETAVCNLGSLNYPVHIVNGRFDEAMVKETVTTAMRMLDNVIDLNFYPTIEARNS
                     NMKHRPVGLGIMGFQDALYMQNIPFASEECVTFSDSSMEVIAYYAILASSELARERGA
                     YQSFKGSKWDRGIFPIDTIALLEAERGIPTGTDRSERLDWSVVRESVKQYGMRNSNTM
                     AIAPTATIANISGVVPGNEPIYKNIYVKANISGDFVIINPHLVDDLKRAGLWSVEMLQ
                     AIKYNDGSLADIPSIPVELKVKYKETFEIDMRWLVKAGAARGKWIDQSQSLNIFFSGT
                     SGQELSDLYVYAWEAGLKTTYYLRSLAATQVEKSTVGTAGTHLRKGTETGAKPVEETP
                     SPVSVAPAEPVATVLAMKSPISTPSPFVKEVRDEAKSDTVPAKPNVKLHVAEDAICES
                     CQ"
     gene            4823..5857
                     /locus_tag="VFS75_00300"
     CDS             4823..5857
                     /locus_tag="VFS75_00300"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEU4677143.1"
                     /translation="MPIRKNAPPVDINKRRAINGEDADVIQLYPMKHRWAWDYYNAAN
                     ANHWLPTEISMQLDIEQWKSQTALTDDERHAFETILGFFTTADSIAANNIVIATYRQI
                     TSPEIRMYLLRQAYEEAIHTHAYQYIVESLAMDESKIFNMYRERDAIYNKASFILSFN
                     EGIFDPTFKTGTFEADQKFLENLCIFSLILEGIFFYSSFAVMFGFQRQHKLTGSAEQI
                     QYIMRDESMHLNFGCQLINTIREEQPELWTVEFQERIVGFIREAARLEYTYAQEVFPK
                     GIFGMNANGFRQYIEHIADRRLERVGLPQQFGVANPFPWMSEAIDLPKEKNFFETRVT
                     EYKTGGALNW"
     gene            complement(5968..6387)
                     /locus_tag="VFS75_00305"
     CDS             complement(5968..6387)
                     /locus_tag="VFS75_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677144.1"
                     /translation="MRLYFAFLWQRVALVEARMTWKRYRSFTLRQMRHELPELREEMR
                     IKHERWMMVPVIYWIDEYFCNPGCPHCGNRLLQRWQTIRYVSPAGELSRTQWLVLHCH
                     HCGKDSNGSMLFSRDRIRYPDPPPRRPERIHARVTTP"
BASE COUNT         1404 a         1923 c         1911 g         1262 t
ORIGIN      
        1 cttatactta tttcttttca ctttaaataa ttctaacatt ctgataaata cttttaatgg
       61 acttatctgg aagtgattct ttctatccga tagcaagact ccaatgggga atgtatatcg
      121 ttgcatcatt ctttctctca aatatagttc taacttcctc tacgacgtcg cccagatcag
      181 ccaaccccga agcgaaagct tcggggttgt tgctgtctgg agcggctact gggaatcgaa
      241 cggcggagcc ggggcccctt tgggccggcg agccgcgtcc agtgttctta tgagcgaagc
      301 gaattagaaa aagcgggacg atccagtagc cgcctcgtta tcccccttcc catttgcagg
      361 agggaagggc tcttgtcaga atgtatctat gagaatcaga atgctcgaat cattcggtaa
      421 gccggagatg agtccgctcg agacatacgt tgaggagttc aagaaggatt acgaggagct
      481 cacgcgcctc gcggcggcat ccgccgagca cgcggcggga ctggggtacg gcaccgatcg
      541 cgacgagaag gcgaaggaga tgcgcgagcg gttcgaggcg atgctcgagg acgtcctcgg
      601 cccgaagaaa gaaggtgaag gagatgcgca gtcggagctc acgcttcagt agacgacgtt
      661 tcgacatagg aaaaggcgac ccatgggtcg ccttttacgt cagtctccgt tatggaacgc
      721 gtggctcgtg agcgcctcgc cgccgatgta cgaatcgatg tgctgcttct cgagcgcgcg
      781 cgcctccgcc tttcgacggt ttttggcgtg ccgctcgtac cgcgtgcttc tgaggtgctt
      841 tcggagcacc cctgtgagga tgacgatgag gaccaccgcg gcgagggtga agtacaccga
      901 cggttcgtga agccatgccg ccaccatttc catgacgctg tctcccttat tgtgatgagc
      961 cggactgccg tgaacgcatc cgtatccgac ggatgagcac tatttataca acaggtatgg
     1021 gctgcggtca aaggcggcct ttggtacaat gtcgctatga gcacggcaga catcgtgggc
     1081 ggcgccatcc tcctcttcgc catgggagcg gcgctcggct actacacggt ccgcatcatc
     1141 gtcggcgtgt agggtaagcg gccgcgaatt tccactatga gaaaattctt caacgagttc
     1201 aagcagttcg cgatgcgggg gaacgtcctc gacctcgcta tcgcggtcgt catcggtggt
     1261 gcgttcaata agatagtctc gtcgctcgtc gataccatca tcaccccggc gatcggtctc
     1321 ctcatcggcg gtgtcgactt ttcggccctt agtttcaccg ttggtgcgtc cacgttcaag
     1381 tacggactct tcgtccagaa cgtcgttgac ttcgtcctca tcgcgctcat catattcctc
     1441 gtcgtgaagg ccatgaaccg gttccatcgc cgcgaggagg aagaagagga gaagcttccg
     1501 cccgagccgc cggaggaggt caaactgctc cgcgagatac gagacgagct caaaaacggc
     1561 tcgcgttcgt gattcggaaa cagagatacg cctctcgcaa gtcttgcagg cggaaccctt
     1621 ttgttgctat ccacaggacg gcttgtgcgc gggacgaagc gtccctgcgg aaaatggtcg
     1681 ttttctccct gtcctacccc gaaactgtgg atttccgcca ttatttgtca tgattcgggg
     1741 cggtggtatc atcaaatcac gacataatgc tcttcgtgtg tcgcttggag atatgaccac
     1801 ttaggaatag gtgggctgtt aaaaaaccct ctccgttttt ccgaccaatg gaccaatgtc
     1861 gggatgggag attaccaacg taagcagaac cggtttatga tcaccaccat cattaagagg
     1921 aacggaacga cggtaccgtt cgatccgacg aagatcgtcg tcgtaatgaa gaaggcgttc
     1981 gcggcggagc aggtgccgat cgacgacgcg gctctcgagc agatgaccgg ccgcgtcgtc
     2041 gcgaacctca cgacgatgtg cgacggcaag gagaactgcc caacggtgga acaggtgcag
     2101 gacctcgtcg agaatacgct catggagcgc ggatacttca cggtcgcgaa ggcgtacatc
     2161 ctctaccggt acaagcagag cgaagagcgt aagcaggagg tcataaagga gatcgaggaa
     2221 cagcgcctta cggttcggcg ctccgacggc tcggtcgagc agttctctcg cgagaagatg
     2281 cgggcgtacc tcgccgagtt cgtttacggg tacgagaagg agattgacct cgacgaactc
     2341 gtctcgcagc tcgagagcga ggtctacgac ggtatcaaga cgtccgagat cgcgaagctc
     2401 gcgaccctcg tgcttcgctc acgtatcgag caagatcccg catactccat cgtggcgtcg
     2461 cgccagctcg cgcggcgcat cgccgacgag gcgatcggga atacccgttc gctctccgct
     2521 gaagagcgaa ccgccgcgta tcgaaacgcg ttcgtacgga acatcaagct cggcgtcgag
     2581 gcgaagaagt acgacgcagg acttctctcg ttcgacctcg agaaggtcgc cgcggctatc
     2641 gagccctcgc gagacgacct cttccggtac ctcggcatgg agacgctcca tgaccgatac
     2701 ttcgttcgcc tcgacgagaa gcgtgtcatc gagacggcgc agatgatgtg gatgcgtatc
     2761 gcgatgggcc tcgcacttaa cgagaaggac aaagagggaa gggcggtgga gttctataac
     2821 gtcatgtcga gactccggtt cgtgccatcg acgccgaccc tcttccacgc cggtacgccg
     2881 catccgcagc tctcgtcatg ctacctcaac gtcgtcgagg acgatctcaa gcacattttc
     2941 aaagtgtacg gcgacaatgc gatgctctcg aagtgggcgg ggggcatcgg caccgcgtgg
     3001 tcgaagctcc gcggcacggg cgccgtcctc aagtcctcgg gcatcacgtc gcagggcgtc
     3061 attccgttcc ttaagatcgc gaacgacgtg acggtcgcca tcaaccgctc gggacggcgc
     3121 cgcggcgcga cgtgcgtcta cctcgagaat tggcacatcg acttcgagga tttcctcgag
     3181 cttcggaaga acaccggcga cgagcgccgt cgcacgcacg acatgaacac cgccgcgtgg
     3241 gtttccgacc ttttcatgaa gcgcgtgaag gaggatggcg attggacctt gttctccccg
     3301 gatgagacgc ccgacctcac cgagacgtac gggaagcagt tcgaggaggc gtacgagaag
     3361 tacgaggaga tggcggaccg cggagagctt cgactcttta agcgcataaa agcgcgcgac
     3421 ctctggaaga agcacctctc gatgctcttc gagacgggcc acccgtgggt cacgttcaag
     3481 gatccatcga acgttcgctc gccgcaggac catgtcggcg tcgtccacaa ttcgaatctc
     3541 tgcacggaga taacgctgaa tacgtccgcg gaggagaccg cggtctgcaa cctcggttcg
     3601 ctcaactatc cggttcacat cgtgaacggc cgcttcgacg aagcgatggt taaggagacg
     3661 gtcacgaccg cgatgcgcat gctcgacaac gtcatcgacc tcaatttcta cccgacgatc
     3721 gaagctcgca attcgaacat gaaacaccgc cccgtcggcc tcggtatcat gggattccag
     3781 gacgccctct acatgcagaa tatcccgttc gcgtccgagg agtgcgtcac gttctccgac
     3841 agttcgatgg aggtcatcgc gtactacgcg atactcgcat cctccgagct cgctcgcgag
     3901 cgcggcgcgt atcagagttt caaaggctcg aagtgggacc gcggcatttt ccccatcgac
     3961 acgatcgcgc tcctcgaggc ggagcgtggc atcccgacgg gcaccgaccg cagcgaacgt
     4021 ctcgactgga gcgtcgtccg cgagtccgtg aagcagtacg gcatgcggaa ctcgaacacc
     4081 atggcgatcg cgccgaccgc gaccatcgcg aacataagcg gcgtcgttcc ggggaacgaa
     4141 ccgatctaca agaacatcta cgtgaaggcg aacatctcgg gcgacttcgt catcatcaac
     4201 ccgcacctcg tcgatgacct gaagcgcgcg gggctctgga gtgtcgagat gcttcaggcg
     4261 atcaagtaca acgacggtag cctcgcggac atcccctcga tccccgtcga gctcaaggtg
     4321 aagtacaagg agaccttcga gatcgacatg cgctggctcg tcaaagcggg cgccgcgcgc
     4381 ggcaagtgga tcgaccaatc gcagtcgctt aacatcttct tcagcggcac ctccggccag
     4441 gagctctccg atctttacgt gtacgcgtgg gaagcgggtc tcaagacgac gtactacctc
     4501 cgctcgctcg ccgcgaccca ggtcgagaag tcgacggtcg gtaccgccgg tacgcatctt
     4561 cggaagggaa cggaaacggg cgcgaagcct gtcgaagaaa cgccgtcacc ggtctcggtc
     4621 gcgcctgcgg agcccgttgc gaccgtcctc gccatgaagt cgcctatctc tacgccgtcg
     4681 cctttcgtaa aggaggtgcg cgacgaggcg aagtcggata cggtccccgc gaaaccgaac
     4741 gtgaagcttc acgtcgccga ggacgccatc tgcgaatcct gccaatagat agtcccttac
     4801 ccagttctct aaccccacga gcatgccaat cagaaagaac gcgcctccgg tcgacatcaa
     4861 taagcgtcgc gcgataaacg gcgaggacgc cgacgtcatc cagctctacc ccatgaagca
     4921 caggtgggcg tgggactact acaacgcggc gaacgcgaac cactggctcc cgacggagat
     4981 cagcatgcag ctcgacatcg agcagtggaa gtcgcagacc gcgctcaccg acgacgagcg
     5041 gcacgcgttc gagaccattc tcgggttctt cacgacggcg gactcgatcg ccgcgaacaa
     5101 catcgtcatc gcgacgtacc ggcagataac gtcgccggag atacgcatgt accttcttcg
     5161 ccaggcgtac gaggaggcca tccacacgca cgcgtaccag tacatcgtcg agtccctcgc
     5221 gatggacgag tcgaagatat tcaacatgta ccgcgagcgt gacgccatct acaacaaggc
     5281 gagcttcatc ctctcgttca acgagggcat cttcgacccg acgttcaaga cgggcacgtt
     5341 cgaggcggac cagaagttcc tcgagaacct ctgtatcttc tccctcattc tcgaaggcat
     5401 cttcttctat agttcgttcg cggtcatgtt cggcttccag cgccagcaca agctcacggg
     5461 gtccgcggag cagatccagt acatcatgcg cgatgagtcg atgcatctca acttcggctg
     5521 ccagctcatc aacacgatca gggaggaaca gccggaactc tggaccgtcg agtttcagga
     5581 gcgcatcgtc ggtttcatcc gtgaggcggc gcgcctcgag tacacgtacg cgcaggaggt
     5641 cttcccgaag ggcatcttcg gcatgaacgc gaacggcttc cgccagtaca tcgagcacat
     5701 cgccgaccgt cgcctcgaac gcgtcggact cccacaacaa ttcggcgtcg cgaacccgtt
     5761 cccgtggatg agcgaggcca tcgacctccc gaaggagaag aacttcttcg agacgcgcgt
     5821 gaccgagtac aagacggggg gcgccttgaa ttggtgagaa gcgatacaaa atcgcttcgc
     5881 gagaccttga gcagtttctg tgaacatatc cttcgtctcc gagtctttgg atcgaaaaaa
     5941 cgcccgagca gtgctcgggc gttttcgtca cggtgtcgtg actcgtgcgt gaatccgttc
     6001 cggccgccgg ggaggcgggt cggggtagcg gatgcggtct cgggagaaga gcatcgaccc
     6061 gttcgagtcc ttgccgcagt ggtggcagtg aaggacgagc cactgcgtgc gggacagttc
     6121 tcccgcgggc gagacgtacc ggatggtctg ccagcgctgg aggaggcgat tcccacagtg
     6181 cggacagcca ggattacaaa agtactcgtc gatccagtag atgaccggta ccatcatcca
     6241 ccgttcgtgc ttgatgcgca tttcctctct caactccgga agctcgtgcc gcatctgacg
     6301 tagcgtgaac gaccggtatc gtttccaggt catacgcgcc tcgacgagcg cgacgcgttg
     6361 ccacaggaac gcgaagtaca gtctcatggc gaaccgcgcg tggacgacgt accggcgcca
     6421 caaacgacga atcgtagcgt acatggtgca cctcgagggg aatcatctgc ggaaagcgta
     6481 cgctccgtcc gtcggtcggt
//
LOCUS       DASTSP010000009        18470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_475226, whole genome shotgun sequence.
ACCESSION   DASTSP010000009 DASTSP010000000
VERSION     DASTSP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18470
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475226"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFS75_00310"
     CDS             complement(<1..559)
                     /locus_tag="VFS75_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4677145.1"
                     /translation="MALVYITRHIPEIGINLLRAAGHTVDVSAKDGVLTPDELRAALS
                     AKPYDAVIIQLTDTIDASVFSAAPSAKIFANYAVGYNNINLDDAKTAGVTITNTPGVL
                     TDTVAEYTIALMCAVTKRISESERYLRAGKYEGWAPELFLGMDLKGKTLGILGAGRIG
                     YEVARRAVLGFGMKVIYNDMKQVDAL"
     gene            602..1636
                     /locus_tag="VFS75_00315"
     CDS             602..1636
                     /locus_tag="VFS75_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEU4677146.1"
                     /translation="MSRSLGWYNEAIMAQQYDFVAIGDITIDAFIELSNEDARVTKDD
                     KGRKKLEMAFGEKLPYKDVTVINAVGNSPNAAVAAHRLGLRSALVANLGHDRNGKDCL
                     DALRTEGVDTDFVKVHEGKKTNYHYVLRYGAERTILIKHEEYPYTLPDFRVPPRFLYF
                     SSIGENGVELHHIAARYLKEHPETRLAFQPGTFQIRLGTKELRDLYAETDIFFCNKEE
                     AQEILQTDESDFKKLLLGIKELGPTTPVITDGPGGAYALDGETAWFMPMYPDPKEPVD
                     RTGAGDSFSSTFVSAMVLGHDVRTALSWGPVNSMSVVQYIGAQAGLLSRDKIEEYLRE
                     APHDYVPKEV"
     gene            complement(1683..2684)
                     /locus_tag="VFS75_00320"
     CDS             complement(1683..2684)
                     /locus_tag="VFS75_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4677147.1"
                     /translation="MNDTHQLAQHVNPNFFTDRVERVPNRNGYGDGLKEAGTDDERIV
                     ALSADLTASTKTNVFAEAFPKRFIEVGVAEQSMASVASGMAAMGKIPFIASYAMFSPG
                     RNWEQVRTTIAYNGANVKIVGAHAGVSVGPDGATHQAIEDIALTRVIPRMVVIAPCDA
                     NEARKAVRAAAKYVGPVYIRLSREATPVITTVGSPFEIGKAEVYLTRNRVHKRTAGII
                     ATGTILAEAMLAGKRLNERGIGASVMNMATIKPLDTKALLSFAKEHDVIVTVEEHQRA
                     GGLGSAVVEYLSAVLPKWIVRIGVDDEFGQSGEPDELLRHYGLDADSIVRETQKFFG"
     gene            complement(2696..3592)
                     /locus_tag="VFS75_00325"
     CDS             complement(2696..3592)
                     /locus_tag="VFS75_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU4677148.1"
                     /translation="MHYLSDEEVLSLERKANEVRQSIIEMLVEAKSGHTAGPLDMADI
                     FTLLYFEVLKHDPKNPFWNDRDRVILSNGHICPVLYAVMAHAGYFPLEELLTLRKFGS
                     RLQGHPHREHLPGLETSSGPLGSGLSQALGMALAERIDNPYSSKYFYCFLGDGELDEG
                     QVWEAIMHAGKEKVHNLIAIVDRNGIQIDGYTDDVMPLEPLHEKFRAFNWDVQEVDGH
                     NIRAVNDAIGKAQSIYGQPSIIIAHTIPSKGVDVFERDFRWHGNPPGLGPEDRVKKTD
                     QGKVALKKLRTLNGQLRTNECE"
     gene            complement(3636..4289)
                     /locus_tag="VFS75_00330"
     CDS             complement(3636..4289)
                     /locus_tag="VFS75_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677149.1"
                     /translation="MNHIIPAIIPESFQMLEAALSTVRGFGREAQIDVVDGAFVPARS
                     WPFTEGDVSDLARLSPAFILEADLMVEAPEDAIPGFSRFGVRKMVVHAESVRDWDRIR
                     EQRAKLGFSLGLSILNDSPLSLLLDRVSHIDYVQLMGIKTIGVQGEPFDERVLERICA
                     VRAQHPDLMISVDGSVNETTLPKLIEAGADRFVVGSAIFDAPNPAEAYGRLTRIEVL"
     gene            complement(4286..4738)
                     /locus_tag="VFS75_00335"
     CDS             complement(4286..4738)
                     /locus_tag="VFS75_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU4677150.1"
                     /translation="MKVYFGTDHAGYELKNTLLAYVEGELGHEVVDCGAYEENPEDDY
                     PAFVRAAAEAAAKDPHGSRAIVLGGSGEGEAMVANKVPGIRAAVYHGGNLDVVALSRT
                     HNDANVLSLGARFMKKDEAKEAVRLWLETPFSGEARHARRIASIEESS"
     gene            complement(4757..5776)
                     /gene="gap"
                     /locus_tag="VFS75_00340"
     CDS             complement(4757..5776)
                     /gene="gap"
                     /locus_tag="VFS75_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4677151.1"
                     /translation="MAKARVAINGFGRIGRTFFRMSYGHPDFEVVAVNDLGDIKSLAY
                     LLEYDTAYGRSPYSVAVEDNALVIDGRKIAFLSEREPQNLPWGKENIDIVVESTGFFT
                     AYDKAKAHLDAGAKRIVISAPVKTENGDAGKTVLMGVNEGELAGCAITSNASCTTNAA
                     SPLVGILNEAIGIEKAILNTVHAYTASQALVDGPSKKDFREGRAAAQNIVPSSTGAAI
                     ATTMVHGDLKGKFDGISIRVPVIVGSIVDVTFIAKRDTTVEEVNAALEKAAASETWKD
                     VFAVTHDPLVSSDIIGAEHASIADLAFTRVVGGNLVKVLAWYDNESGYTSTLVSHVAK
                     AAKLL"
     gene            complement(5857..7416)
                     /locus_tag="VFS75_00345"
     CDS             complement(5857..7416)
                     /locus_tag="VFS75_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677152.1"
                     /translation="MARHFTITVRVAAIALLSMLSVVPALSVRAAADDFRVRTQVGAD
                     TTAPSIPQNLTATPVATTQIDLAWGESSDNIEVSGYNVWRDDALLATTSTTTYSDTGL
                     SASTTYTYYVTAFDPSFNYSASSSVATATTFAEPEPPTPTSTPGRTTGTRIVPFPEEI
                     TSLEILPERDAVTIRYTTKDYVRSVLRWGRTTSYEIGSLGEQAYSRDHVTRITGLEPG
                     TQYAFSIEGETGLHRSGTMHAGSFTTLPAEDTFPPGNVTGLTATREGSDVVLSWQNPR
                     DADFEKVRIVRSDRFYPVDTQDGWVAYEGTGESVRDENVAVNGYLYYTVFAYDRLGNV
                     SSGAVVSIYMGSGEPPSEEPNPNENAIDLSFGDIVFVQEGVVLPSTGGEVRVDGAKQL
                     TVSIPYGRLPEHLKTIVVTIKESPESDKTLKFLLRVNGEKTTYTGTLAPFSRAGTFPV
                     DVTVFDYKTSQVGYAKGTIVSKLLSSAVGPVGFFDRMRDIGLSYWYAFWFVLLLILLF
                     FLGERIHRREW"
     gene            complement(7420..8193)
                     /locus_tag="VFS75_00350"
     CDS             complement(7420..8193)
                     /locus_tag="VFS75_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677153.1"
                     /translation="MRIAMRDALATSVVALFATVAIFIGLSSAAAAVMESTNYRIESD
                     SINFAGGLSSSTNYTLESTAGEAATGESASASYNLKAGYQQMVTTFISMTAPSSVTMT
                     PSIPGVSGGIANGSTTVTVRTDSAAGYELSIASTESPALTMGADSIADYAPAGADPDF
                     TFSTGATESHFGYSPSGLDTVSRFLDDGAACNTGALDTALSCWDGLSTTSEAIARSSG
                     ANTPDGTVTTVNFRVSVGGSVTQKAGTYTATTTLTAFSL"
     gene            complement(8195..9226)
                     /locus_tag="VFS75_00355"
     CDS             complement(8195..9226)
                     /locus_tag="VFS75_00355"
                     /inference="COORDINATES: protein motif:HMM:NF017810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEU4677154.1"
                     /translation="MQTNVHRIHVLATVFGALFVLACAPSLAYAEDASTTVGSGDSLL
                     NLYPTEGIPGGDAVIGDFVVGPGKVDLTVKPGETKVVKMTVTNRTGERRRFNLHVEDA
                     AGSEDVNTPIVLLGSDRGPYSLKDYVHIPYLTFDLDQNERATIPVTISLPPDAEPGGL
                     YGSVLVDTVSVEANPGIIDNTVPQSAIIARVGTLFFITVPGAVERNGTMKSFTTVPEE
                     TFYASGPIHFGVLFENTGSMHLAPYGEIRVTNMFGDEVGFVELDPWFVLPQSLRLREV
                     TWDRGYLFGRYTATAKINRSYDDTIDEMSYTFWVLPWKPLTAAFIALFIVFFLIRLFF
                     RTFEFKRKG"
     gene            complement(9244..9966)
                     /locus_tag="VFS75_00360"
     CDS             complement(9244..9966)
                     /locus_tag="VFS75_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677155.1"
                     /translation="MYARMNQTHVFSSATNAFVAAALVTALGVLSFFAFEPSIGHSQI
                     NDSFTVSQTITSEISFNVTAADVTMVGTIAGVSGGYATGTTRVSVNTNNATGYNMTLG
                     FSSNAVNAAMVSSSTDASSPYIANYTPAVAGVPDYNFVTNSTGGAGEFGYTVMASTSG
                     EVDPSFMNNTTNCNAGTNETVDRCWLNPTTTPETIINSTGENQSSTTTIKFKVAIPSN
                     PSPAIPSGVYVATGTLTATVNP"
     gene            complement(10065..14195)
                     /locus_tag="VFS75_00365"
     CDS             complement(10065..14195)
                     /locus_tag="VFS75_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677156.1"
                     /translation="MNREHRQPHKIPLDHRLFLILAFLVLFSPLRAAADTVTYTVSDA
                     SFSSSVSGACDTSITNATTTDGAQGTVLTSTCVGKSDTPVVSWTKSLTWEDMGVPVGA
                     TVSSVDGSFKYRVAQESVSATQAAALALNDSGDVSACTTSSLEVSFDPGAVSGTYSTR
                     NATGTVPVKGICAFSYNQVTLHLSLTPATGNNNSATSELRADDVQLVITYMPSLAPTL
                     SGTLYTDEGRSVASAGKTVSVRALNGLDTTLSTTTAADGSWSVDTAGFLGFLNASAPF
                     VVWVDGDPAMRAALVSKATSSVDAISGLDLYQNRVIVSNEATSATTTTIADLSFFDGD
                     DDPDIQYTANGGTLAVNAGQELHVKAGKTFAPGGPVVVNGNASASPTDGSFHIGTGAT
                     YIAGGTTTIAGNLIASSSSLFTAHASGTFFNATTTGKTINTTSTSSLGDVTFSGAGGG
                     WSFLTNATTSDFTVSTGTVSASSGTMTVQGNFVNDGVFDGGGSLRFFGSRGGSISGTL
                     TGTSALGDVIVEANMASTSDGLTEPYGNFVSDLPYPNLTNSGNAMASDDQYATGGFGA
                     GSLYAFTMDTSGMDSISGMEVRVEASGLCADAEVRINSSYSKLFTAASEAVYILGGPT
                     DMWGSTIAPTGSTTQLSVELITPASCTYSFDNVAVNVYGRTWSFASAASTTDLMIAAS
                     STLVAPPMLSIAGDYTNNGTFEAGSGTTTLGGATIQVLSGTMVGTSAFSNLEIANTSG
                     NGINSQSIVFAGPASTTDTLTMRASSSAAFSGATSTFQNIYLQGAPGQLIYLRSVNIL
                     GSEGVYNFYVPGTQKAVQYVDVRYANACPSTITAADGTSVDSGNNTCWDFGGDAPVIS
                     SAVDQAFSYGEDATDISTITVYDALNAPTITAMNDIRITIATSTVPMRWDAADTNAIF
                     GGTAAAKVSNPVSYEDGDATLVIPVSSDFAAGDSLTISELRFADFTGTTSPAVALSLY
                     TGGAGDTVADDADSKMVGISGSLTIDEHDAGQVGDQFTTSHANGVVLYRMKLTPAMEN
                     ANISSLAFDLRGVEGLAAGDFTNLALYRDVNGNGSYDAGDVAVGGSGVPSVSGQTGAI
                     TFGTPFTASTSAEYLLRGDVDGIGPKGSVVVALTGEGIDATGEMTGVAITEAGTPSTV
                     QHLRNGSRGGGGSGSAVGGPAPAGDGVKTGGGGGGGESIGNEFGFMAPTTSNGSWTTG
                     ENAYVSDDAYAVVSGNSAVENYGNFNFAIPAGSQITGIAVKLEARTDENSPIMVILSW
                     DGGPSVTAFQLTSGTSATDAVYTVGGPADTWGRTWSSSDFNNGNFTVALTPTASLFGF
                     TYLDAVQVRVYYEATGGGAGGGGFVSRDENRYLALVYGAAGEALFRLVRSFITAIF"
     gene            complement(14192..>18470)
                     /locus_tag="VFS75_00370"
     CDS             complement(14192..>18470)
                     /locus_tag="VFS75_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEU4677157.1"
                     /translation="STSIDTSAELAALLTDETGTAGRVVFSASPTLSGLLTFANASGT
                     ALTVDGTAFLSDLSVSGTATTSALIAGSARITGGTSFPTSPSEGTLFFSTASSTLYVY
                     ENGKWQTDRSTATYLVAASDTTNKESADFVATGTNDQDTINAALSALPSTGGLVYLLP
                     GTYSINNSINVLQSSTTIMGGGANTLLALTATSTNWGVIASSGKVGVTVRDLTIDGSA
                     AVGSTQYGIVLGSSYASVENVSFRRLRGYGIYAGSTYSRIENNSFTGDTQAGVYLTAS
                     SQYNVITGNTFTENGLYGVFGNSTSGNNVITGNSFKGNTRAIQFSASPNVTISGNTFI
                     GSSQYAIVSDSGSGTVITGNVFYGNTNGGAYATIYVNDNYDLITGNTIQATSTDPLIQ
                     IQGGTGNTIANNTYVNPDTTTYVSDGGTDTRYDAYDRVTLGTPTSRAYSIFTINASSS
                     SSTVEVAQKGTGSILDLYNATTRVLTVTNAGRLGLGTTTPAELLSVAGNMHLTGALYD
                     GTYNPGASGMVLQSVGSGVAWVATSTLGITGGSGSSNWVVSNGALTPSSTVGFSVHAS
                     STIGDGTQAGGLTINGGATTTGTAYFGGNVGIGSSTPTAILTVASTSLTGTSPLFILA
                     TSTTSNILKVLANGQVSLGGAAVNGYRLSVRDGATLLAGTGATLTVKKGIGSPNFLST
                     ESVASFDNETFPGSGVAVNFFTVSSASSTIYFGNTALPEQGSISYGVNSGTPTSQRLS
                     FRVNSAERMRIDGNGNVGIGTTTPTSLLTMDVQSLSGSVVGGIKQSFGFVNAVASAVS
                     YGNETYITTNPTATSTLVGSMIRIADTSTLGNTVRGFEAQAFRGTNTNGENTGISGYG
                     RTFGVRGTTLGDAGSVYLPAGVFAETQGTAQGNALRAYSGTITSEALVSLFQDTSEFT
                     GTGLEMNFGNAGGSFLATSTAKFIDLKVAGTSKFIIAANGSTTIGDGSVAAGLQIPYG
                     GICVDNDGSCVSTTTGQIRSVTSATGNSDLAEMYFSKEALRSGEIVALAGGLSIEKAD
                     ASSSPNVIGVVSTKPGLVLGFDDTSLVTGEAPYPVGLKGRVPIRLSTENGPIKKGDRI
                     TLSSVPGVGMKATGSARVVGIALEDYDGRYAYSDGFLNQFGDDMVKETIKAKKDAYDP
                     RAQDGCYYGGGAPEGEVGCTPRDVREIPPELMDEETLRDVALRELADEKPETLTLEGG
                     EEVAIGQALMFIELTTYLADAESEILRELAAPIPGSEETLWSRLKTLATNFVDGVLTV
                     LEIRADRIVVSDTLCVDDVCVTSDELRALLNSTNGSESGTSSNADESEPSSPDQSETM
                     QGDVGDSATTTDAVEESASNDTASSSPAEPTVAEDDGTTSESEPAAGPSASEDVAAAD
                     EVSGENLDESQIPEAPSVDASVEAPPEKGPSEATADVGGEAPNGQSI"
BASE COUNT         3340 a         5740 c         5776 g         3614 t
ORIGIN      
        1 cgagcgcgtc aacctgcttc atatcattgt agatgacctt catcccgaag ccgagaaccg
       61 cgcgccgtgc gacctcgtac ccgatgcgtc cggcaccgag gatgccgagc gtcttcccct
      121 tgaggtccat gccgaggaag agttccggcg cccatccctc gtacttcccc gcgcgaaggt
      181 accgttccga ctcggaaatg cgcttcgtga cggcgcacat gagcgcgatc gtgtactccg
      241 cgaccgtatc ggtgaggacg cccggggtgt tcgtgatggt gacgcccgca gtcttcgcgt
      301 catcgaggtt tatgttgttg tagccgaccg cgtagttcgc gaatatcttg gcgctcggcg
      361 ctgcgctaaa gacgctcgcg tcgatcgtat cggtaagctg gatgatgacc gcatcatacg
      421 gcttcgcgga gagcgcggcg cggagctcat cgggagtgag gacgccgtcc ttcgcgctta
      481 cgtcgacggt gtggcccgcg gcgcgaagga gattgatgcc gatctcgggg atgtgccgcg
      541 tgatgtaaac gagtgccatg gtgttctctg tgtcgctatc gaataaccac atcactatac
      601 catgtcacgc tctctcggat ggtacaatga ggccatcatg gcacagcagt acgacttcgt
      661 ggcgatcggt gacatcacga tcgacgcgtt catcgaactc tcgaacgagg acgcgcgggt
      721 aacgaaggac gacaaggggc gaaagaagct cgaaatggcg ttcggcgaga aactcccgta
      781 caaagacgtc accgtcataa acgcggtcgg caactcgccg aacgcagccg tagcggcgca
      841 ccgtttgggc ctccgttccg ccctcgtcgc gaacctcggc cacgaccgaa acggcaaaga
      901 ctgcctcgat gcgcttcgta ccgaaggcgt cgacaccgac ttcgtgaagg tccacgaggg
      961 caagaagacg aactaccact acgtccttcg ctacggtgcg gagcgtacca tcctcatcaa
     1021 gcacgaggag tacccgtaca cgcttcccga ttttcgagtg ccgccccgct ttctctactt
     1081 ctcgtcgatc ggcgagaacg gcgtcgaact ccaccacatc gccgcgcggt acctcaaaga
     1141 acaccccgag acgaggctcg ccttccaacc cggtacgttt cagatacggc tcggtacgaa
     1201 ggagcttcgc gacctctacg cggagaccga catcttcttc tgcaacaagg aagaggcaca
     1261 ggagatcctc cagaccgacg agagcgactt caagaaactt ctcctcggca taaaggagct
     1321 cggtccgacg acgcccgtca taaccgacgg accaggcggc gcgtacgcgc tcgacggaga
     1381 gaccgcgtgg ttcatgccga tgtaccccga cccgaaggag ccggtcgatc gaaccggtgc
     1441 gggtgattcg ttctcgtcga cgttcgtgag cgcgatggtc cttggccacg atgtgcgaac
     1501 cgcgctctca tggggaccgg tgaactcgat gtcggtcgtc cagtacatcg gcgcgcaggc
     1561 ggggctcctc tctcgcgata agatcgagga gtacctccgc gaagcgcccc acgattacgt
     1621 cccgaaggag gtctaaaaga tcccgcagcg cgggatcttt tcctacatgg ccattcatcg
     1681 tatcaaccga agaacttctg cgtctctctg acgatggaat ccgcatcgag accatagtgt
     1741 cgaaggagct catccggttc gccggactgc ccgaactcgt catcgacgcc tattcgcacg
     1801 atccacttcg gaagaaccgc cgaaaggtac tcgacgacgg cgctcccgag gccgcccgcg
     1861 cgctgatgct cttcgaccgt cacgatgacg tcatgctcct tcgcgaacga gaggagcgcc
     1921 ttcgtgtcga gaggctttat cgtcgccatg ttcatgaccg acgccccgat accgcgctca
     1981 ttgagtcgct tccccgcgag catcgcctcc gcgaggatgg taccggtcgc gatgataccc
     2041 gcggtccgct tgtgaacgcg gttacgcgtg aggtagacct cggccttccc tatctcgaag
     2101 ggcgaaccga ccgtcgtgat gaccggcgtc gcctcgcggg agagccggat gtagacgggt
     2161 ccgacgtact tcgccgccgc gcggaccgct ttcctcgcct cgttcgcatc gcacggcgcg
     2221 atgaccacca tgcgaggaat gacgcgcgtg agcgcgatat cctcgatggc ctggtgcgtc
     2281 gcgccgtcgg gtccgaccga cacgcccgcg tgcgctccga ctattttcac gttcgcgccg
     2341 ttgtacgcga tcgtcgtacg tacctgttcc caattccgtc cgggcgagaa catcgcgtac
     2401 gatgcgatga acggtatctt tcccatcgca gccataccgg aggcgacgga ggccatcgat
     2461 tgctccgcga caccgacctc gatgaagcgc ttcgggaacg cctcggcgaa gacgttcgtc
     2521 ttcgtcgacg cggtgaggtc cgccgagagc gcgacgatgc gctcatcgtc cgtacccgcc
     2581 tccttgagcc cgtcgccgta gccgtttcga ttgggcacgc gttcgactct atcggtgaag
     2641 aagttcggat tgacgtgctg tgcgagttgg tgtgtgtcgt tcatatgatg aaatgtcact
     2701 cgcactcgtt cgtgcggagc tggccattca gcgtccgtaa tttcttgagc gcgaccttcc
     2761 cctggtccgt cttcttcacg cggtcctcgg ggccgagacc gggagggttc ccgtgccagc
     2821 ggaagtctcg ctcgaagaca tctacgccct tcgagggtat ggtgtgcgcg atgatgatgg
     2881 acggttggcc gtagatcgat tgcgccttcc cgatcgcgtc gttcaccgcg cggatattgt
     2941 ggccgtcgac ctcctggacg tcccaattga aggcgcggaa cttctcgtgc aagggttcga
     3001 gcggcatgac gtcgtcggtg tacccatcga tctggatgcc gtttcggtcg acgatcgcga
     3061 tgaggttgtg aaccttctcc ttacccgcgt gcataatcgc ctcccacacc tgtccttcgt
     3121 cgagttcgcc atcgccgagg aaacagtaga agtacttcga cgagtacggg ttgtcgatcc
     3181 gctccgccaa agccatgccg agggcctgcg aaaggccgga gccaagaggg cccgagctcg
     3241 tctcgagccc gggaaggtgc tcccggtgcg gatgcccctg gagtctcgag ccgaactttc
     3301 gaagcgtgag gagctcttcg agggggaaat acccggcgtg cgccatgacg gcgtagagga
     3361 cgggacagat gtggccgttc gagagaataa cgcgatcacg gtcgttccaa aaggggttct
     3421 tcgggtcgtg cttgaggacc tcgaagtaga ggagcgtgaa gatgtcggcc atgtcgaggg
     3481 ggccggccgt gtggccgctc ttcgcctcga cgagcatctc gatgatgctc tgtcggacct
     3541 cgttcgcctt cctctcaagg gaaagaactt cctcgtcgga caggtagtgc atgtctacag
     3601 tataccgcgc gggtggcgga tgcggccctg cctgttcaca gaacctcgat gcgcgtgaga
     3661 cggccgtacg cctctgcagg gttcggtgca tcgaatatcg cggagccgac gacgaagcgg
     3721 tccgcaccgg cctcgatgag cttagggagc gtcgtctcgt tcacgcttcc atcgacgctt
     3781 atcatgaggt cggggtgctg ggcgcgtacc gcgcagatgc gctcgagcac gcgctcgtcg
     3841 aacggttcgc cctggacgcc tatcgtcttg atacccatga gctgtacgta gtcgatgtga
     3901 gagacgcggt cgagaaggag cgaaagcgga gagtcgttca atatggaaag tccgagcgag
     3961 aagccgagct tcgcccgctg ttctcgtatg cggtcccagt cgcggacgct ctccgcgtgg
     4021 acgaccatct tccgcacgcc gaaacgagag aatcccggta tcgcatcctc cggcgcttcg
     4081 accatgaggt ccgcctcgag gataaaagcc ggcgaaagcc gcgcgaggtc ggaaacgtcg
     4141 ccctccgtaa acggccaaga gcgcgcggga acgaacgcgc cgtcgacgac gtctatctgc
     4201 gcctcccgtc cgaagccgcg cacggtcgaa agcgccgctt cgagcatttg aaacgactcg
     4261 ggaatgatcg ccggaatgat gtggttcatg aagactcctc gatcgatgcg atacgccgcg
     4321 cgtgccgcgc ttccccgcta aacggcgtct cgagccagag acgcaccgcc tccttcgcct
     4381 cgtccttctt catgaaacgt gcgccgagcg agaggacgtt cgcgtcgttg tgggtgcgcg
     4441 agagcgcgac gacgtcgaga ttacctccgt ggtacacggc cgcccggatg ccaggaacct
     4501 tgttcgcgac catcgcctca ccctcgcccg agccgccgag aactatcgcg cgagaaccgt
     4561 gagggtcctt cgccgccgcc tcggccgcgg cgcgcacgaa cgccggataa tcgtcctccg
     4621 ggttctcctc gtacgcgccg cagtcgacga cctcgtggcc gagctcaccc tcgacgtacg
     4681 cgaggagcgt gttcttgagt tcgtaccccg cgtgatcagt gccgaagtat accttcatga
     4741 ttgcggtcct tagctcttaa agaagcttcg ctgccttcgc tacgtgcgac accaaggtcg
     4801 acgtgtatcc cgactcgttg tcgtaccatg cgagcacctt gacgaggttg ccgccgacga
     4861 ctcgcgtgaa cgcgaggtcc gcgatcgacg cgtgctcggc accgatgatg tccgaggaga
     4921 ccagagggtc gtgcgtcacc gcgaacacgt ccttccacgt ctcggacgcg gccgccttct
     4981 cgagagcggc gttcacctcc tcgaccgtcg tgtcgcgctt cgcgatgaac gtcacgtcga
     5041 cgatggaacc gacgatgacg ggaacgcgta tcgagatgcc gtcgaacttc cccttgagat
     5101 cgccatggac catcgtcgtc gcgatcgcgg cgccggtcga tgacggtacg atgttctgcg
     5161 cggcggcgcg accttcgcgg aagtccttct tcgaagggcc gtcgaccaat gcttgcgagg
     5221 cggtgtacgc gtggacggtg ttgaggatcg ccttctcgat gccgatcgct tcgttcaata
     5281 ttcccacaag ggggctcgcc gcgttcgtcg tacacgacgc gttcgaggtt atggcacagc
     5341 ccgcgagctc accctcgttc acacccatga ggaccgtctt ccccgcgtcg ccgttctccg
     5401 tcttcaccgg cgcggagatg acgatgcgct tcgcgcccgc atcgagatgc gccttcgcct
     5461 tatcgtacgc cgtgaagaag cccgtcgatt cgacgacgat gtcgatgttc tccttccccc
     5521 atggaaggtt ctgcggttcg cgctccgaga ggaacgctat ctttcgccca tcgatgacga
     5581 gcgcgttgtc ctcgaccgcg accgagtagg gactccggcc atacgcggtg tcgtactcga
     5641 gaaggtatgc gaggctctta atgtcgccga ggtcgttcac cgcgacgacc tcgaagtccg
     5701 ggtggccgta cgacatgcgg aagaacgttc tcccgatgcg accgaacccg ttaatcgcca
     5761 cccgtgcttt tgccatatgt gccgtgtatg tatatcaaac tggtaactct tgctcccagt
     5821 ataccccagc cctatgtatg aacgatgaag atatgctcac cactcgcgcc gatggatgcg
     5881 ttcaccgagg aaaaagagaa gaataaggag caggacgaac cagaacgcgt accagtacga
     5941 gagcccgatg tcgcgcatcc ggtcgaagaa accgacgggt ccgacggccg acgagagaag
     6001 tttcgagacg atcgtgccct tcgcgtagcc gacctgcgac gtcttgtagt cgaagacggt
     6061 gacgtcgacg gggaacgtcc ccgcgcggct aaacggcgcg agcgttccgg tataggtcgt
     6121 cttctcgccg tttacgcgaa ggaggaattt gagcgtcttg tcgctctcgg gactctcctt
     6181 gatggtgacg acgatggtct tgagatgctc cgggaggcgg ccgtacggga tagatacggt
     6241 gagttgcttc gcaccgtcga cgcgcacctc gccgccggtc gagggaagaa cgacgccttc
     6301 ctggacgaac acgatgtcgc cgaaggagag gtctatcgcg ttctcgttcg ggttcggctc
     6361 ctcggacggc ggctcgccgc tccccatata gatggagacg acggcacccg aggatacgtt
     6421 gccgagtcgg tcgtacgcga agaccgtgta gtagaggtac ccgttcacgg cgacgttctc
     6481 atcgcgcact gattcgcccg taccctcgta cgcgacccag ccgtcttgcg tgtcgaccgg
     6541 gtagaagcgg tcgctccgga cgatgcggac cttctcgaag tccgcgtcgc ggggattttg
     6601 ccacgagagc acgacgtcgc ttccctctcg cgtcgcggtt aggccggtca cgttccccgg
     6661 cgggaaggtg tcttcggcag gtagcgtcgt gaaggagccc gcgtgcatcg tgccgcttcg
     6721 gtgaagtccg gtctcccctt ctatggagaa cgcgtactgt gtgccgggct cgaggccagt
     6781 gatcctcgtc acgtggtcgc gcgagtatgc ctgctcaccc aatgaaccga tctcgtacga
     6841 tgtggtccgc ccccaccgaa ggaccgaacg tacgtagtct ttcgtcgtgt accggatggt
     6901 tacggcatcg cgctccggga gtatctcaag ggaggtgatc tcctcgggaa acggtacgat
     6961 acgcgttccc gtcgtcctcc ccggcgtcga ggtcggcgtc ggcggttccg gttcggcgaa
     7021 cgtcgtcgcc gtcgcgacgg acgacgacgc ggagtaattg aaagacgggt cgaacgcggt
     7081 cacgtagtag gtgtacgtcg tcgaagcgga gaggcccgtg tcgctgtacg tcgtcgtcga
     7141 ggtcgtcgcg aggagcgcgt cgtcgcgcca tacgttgtac cccgagacct ctatattgtc
     7201 gctcgactcg ccccacgcga ggtctatctg cgtcgtcgcg accggcgtcg cggtgagatt
     7261 ctgcggtatg gagggcgccg tcgtatcggc accgacctgc gtacgaaccc taaagtcgtc
     7321 ggccgccgcg cgaaccgaca gcgcgggcac gacactaagc atagaaagga gcgctatcgc
     7381 ggcgactcga acggttatcg tgaagtggcg cgccatacgt tagagcgaaa acgcggtgag
     7441 ggtcgtcgtc gcggtgtacg tacccgcctt ctgcgtgacc gaccctccga ccgatacccg
     7501 aaagttcacg gtcgtcacgg ttccgtcggg cgtgttcgcg ccggatgagc gcgcgatcgc
     7561 ctccgacgtc gtgctcaagc cgtcccagca cgagagcgcg gtatcgaggg cgccggtatt
     7621 gcacgcggcg ccgtcgtcga ggaagcgcga gacggtatcg agaccggagg gtgaataccc
     7681 gaagtgactc tcggtggcgc cggtcgagaa cgtaaagtcg gggtccgccc ccgcgggcgc
     7741 gtagtccgcg atggagtcgg cccccatggt gagcgcggga ctctccgtcg atgcgatcga
     7801 aagctcatac ccagcggcac tgtcggtgcg cacggtgacc gtggtcgaac cgttcgcgat
     7861 gccgccggag acgcccggga tagacggcgt catcgtgacc gacgacggcg cggtcatcga
     7921 gatgaaggtc gtcaccatct gctggtagcc ggccttgagg ttgtagctcg cgctcgccga
     7981 ttccccggtc gcggcctccc ccgccgtcga ttcgagcgtg tagttcgtcg aggacgagag
     8041 cccgccggcg aagttgatgc tatcgctctc gatgcggtag ttcgtgctct ccatgaccgc
     8101 tgccgcggcg gacgagaggc cgatgaatat ggcgaccgtg gcgaaaagag cgacgactga
     8161 tgtggcgagc gcgtcacgca tggcgatacg catgttatcc cttccgcttg aactcgaacg
     8221 tcctgaagaa gaggcggata aggaagaaga cgatgaagag agcgatgaac gcggcggtga
     8281 gcggcttcca tggaaggacc cagaacgtgt agctcatttc gtcgatcgta tcgtcgtaac
     8341 ttcgattgat cttcgcggtc gccgtatagc ggccgaagag atacccgcgg tcccacgtga
     8401 cctcgcgaag acgaagcgac tgtggaagga cgaaccacgg atcgagctcg acgaagccca
     8461 cctcgtcccc gaacatgttc gtgacgcgta tctcgccgta gggtgcgagg tgcatagaac
     8521 ccgtgttctc gaagagaacg ccgaagtgga tggggccgct cgcgtaaaac gtctcttcgg
     8581 ggacggtcgt gaacgacttc atcgtaccgt ttcgctcgac ggctcccggt acggtgatga
     8641 aaaagagggt gccgacgcgg gcgatgatgg cggactgcgg gacggtattg tcgatgatac
     8701 ccgggttcgc ttcgaccgag acggtatcga cgagcacgct cccgtagagg ccgcccggtt
     8761 cagcgtccgg tgggagcgat atcgtgaccg gtatggtcgc gcgctcgttc tggtcgaggt
     8821 cgaatgtgag gtacgggatg tggacgtagt ccttgagcga atacggaccg cggtcgctcc
     8881 ccaagaggac gatcggcgtg ttcacgtcct cggaccccgc cgcatcctcg acgtgaaggt
     8941 tgaagcgtcg tcgttcgccg gtacggttcg tcaccgtcat cttcacgacc ttcgtctcac
     9001 cgggcttcac ggtgaggtcg accttgccgg gccctacgac gaaatcgccg atgaccgcgt
     9061 cgccgccggg gatgccctcg gtcggataga ggttgaggag cgagtcgccg ctgcctacgg
     9121 tcgtcgaggc gtcctcggcg tacgcgagcg atggggcgca ggcgagaacg aaaagtgcac
     9181 cgaacacggt cgcgaggacg tggattcggt gcacgttagt ttgcataggt tagcggtact
     9241 ctactatggg ttcaccgtgg cggtcaacgt accggtcgcc acgtagacgc cggaggggat
     9301 ggcgggcgac gggttactcg ggatcgcgac cttgaactta atggtcgtcg tcgaactctg
     9361 gttctcgccg gtcgagttga tgatcgtctc gggggtggtc gtcgggttga gccagcaacg
     9421 atcgaccgtc tcgttcgttc ccgcgttaca gttcgtcgtg ttgttcatga acgacgggtc
     9481 gacctcgccg gaggtcgacg ccatgaccgt gtagccgaac tcgccggcgc ctccggtcga
     9541 gttcgtcacg aagttatagt cggggacgcc ggcgaccgcc ggtgtgtagt tcgcgatgta
     9601 cggggacgac gcgtcggtcg aactcgagac catggcggcg ttcacggcgt tcgacgagaa
     9661 gccgagcgtc atattgtagc cggtcgcatt gttcgtgttc acggagaccc gcgtcgtacc
     9721 ggtcgcgtag cctcctgaaa cgccggcaat ggtaccgacc atcgtgacgt ccgcggcggt
     9781 gacgttgaac gatatctcgc tcgtgatcgt ctgcgacacg gtgaaactgt cgttgatctg
     9841 actatggccg atcgacggct cgaacgcgaa gaacgaaagg acgccgagcg ccgtgacgag
     9901 cgccgcggcg acgaacgcgt tcgtggccga gctgaataca tgggtctggt tcatgcgtgc
     9961 gtacatcgaa aataatgctt acctaacaac ttcctatcac gttcattgta tcttttttcc
    10021 gcacaaaaaa ccgtttttac gaaagattac tgtgaataac gggcctagaa gatcgcggtt
    10081 atgaacgagc gcacgaggcg gaagagcgcc tcccccgccg cgccgtagac cagggcgaga
    10141 taccgattct cgtctcgcga tacgaatccc ccgccgcccg caccgcctcc ggtcgcttcg
    10201 tagtacacgc gaacctggac agcgtcgagg tacgtaaaac cgaatagaga ggccgtcgga
    10261 gtgagcgcca cggtgaaatt accgttgtta aagtcagagg aagaccatgt ccgtccccac
    10321 gtgtccgccg gaccgccgac tgtgtagacc gcatcagtgg cactcgtccc ggacgtaagt
    10381 tggaacgcag ttacggatgg cccgccgtcc caggacaata tgaccatgat ggggctattc
    10441 tcgtcagttc gagcctcaag ctttacggcg ataccagtga tctggcttcc ggcgggaatc
    10501 gcaaaattga agtttccgta gttttcaacc gccgaatttc cggacaccac cgcgtacgca
    10561 tcatctgaaa cgtacgcgtt ctctcccgtg gtccaggacc cgttactcgt cgtcggcgcc
    10621 atgaacccga actcgttccc gatcgattcg ccgccccctc ctccaccacc agtcttcaca
    10681 ccgtctcccg cgggggcggg gccgccgacc gcgctccccg acccgccgcc gccccgagag
    10741 ccgtttcgga ggtgttgcac ggtcgacggc gttcccgctt cggtgatggc gacaccggtc
    10801 atctctccgg tcgcgtcgat gccttcgccg gtaagcgcga caacgacgct ccccttcgga
    10861 ccgatgccgt cgacgtcgcc tcggaggagg tactccgccg aggtcgaagc ggtgaacggc
    10921 gtcccgaacg tgatggctcc cgtctggccg ctcacgctcg gaacgccgct tcctcctacg
    10981 gcgacgtcgc cggcgtcata ggagccgttc ccgttcacgt cgcggtagag cgcgaggttc
    11041 gtgaagtcgc ccgcggcgag accttcgacg ccgcgaaggt cgaaggcgag ggacgagatg
    11101 ttcgcgttct ccattgccgg cgtgagcttc atgcgataga ggactacgcc gttcgcatgc
    11161 gacgtcgtga actggtcgcc tacctgccca gcgtcgtgct cgtcgatggt gagcgagccc
    11221 gagataccga ccattttgct gtcggcgtcg tccgcgacgg tgtcgcccgc gccgcccgtg
    11281 tacagagaga gcgcgacggc cgggctcgtc gttccagtga agtccgcgaa tcgcaattcg
    11341 gagatggtga gagaatcgcc cgcggcgaag tcgctcgaaa ccgggatgac gagcgtcgcg
    11401 tcaccgtcct cgtaggaaac cgggttcgac accttcgccg cggcggttcc accgaagatc
    11461 gcgttcgtgt cggcggcatc ccatcgcatc ggcaccgtac tcgtcgctat cgtgatacga
    11521 atgtcgttca tagcggtgat ggtcggcgcg ttaagcgcgt cgtacaccgt tatcgtggag
    11581 atatccgtcg catcctcacc gtatgagaac gcctggtcga cggccgacga gatgaccggc
    11641 gcgtcaccgc cgaaatccca gcacgtattg ttccccgaat cgacggatgt cccgtccgcg
    11701 gccgtaatgg tcgagggaca tgcgtttgcg tatcgtacgt cgacatactg cacggccttc
    11761 tgcgttcccg gcacatagaa gttgtataca ccttcagacc ctaatatatt gactgaccgc
    11821 agatatatga gctggcccgg agcaccttgt aaataaatgt tctggaaggt gcttgtcgcg
    11881 ccagaaaaag ccgcggaact cgatgctcgc atggtgagcg tgtccgtggt cgatgcaggt
    11941 ccggcgaaga ctatgctttg cgaatttatg ccgtttccgg acgtgttcgc gatctcgaga
    12001 ttactgaagg cgctggtacc gaccatagtg ccagagagca cctgaatcgt agcgccgcca
    12061 agcgtcgtcg ttccgcttcc cgcctcgaag gtaccgttat tcgtgtaatc tccggcgata
    12121 gagagcatgg gcggcgcgac gagcgtcgaa ctcgcggcga tcatgagatc ggtcgtcgac
    12181 gccgccgaag cgaacgacca ggttcggccg tacacgttca ccgcgacgtt atcgaaagag
    12241 tatgtacagc tcgcgggcgt gatgagctcg accgatagtt gcgtcgtcga accggtcggt
    12301 gctatggtgc tcccccacat gtcggtcggc cctcccaata tgtacaccgc ttcgctcgcg
    12361 gcagtgaaca acttcgagta ggagctgttg attcgcacct ccgcatccgc gcaaagcccg
    12421 ctcgcttcga cgcgcacttc cataccggag atggaatcca ttcccgatgt gtccatagtg
    12481 aacgcgtaaa ggcttcccgc cccgaaccct cccgtcgcgt actgatcgtc ggacgccatc
    12541 gcgtttcccg agttcgtgag gttcggatac ggcagatcgc tcacgaagtt cccgtacggt
    12601 tccgtaagcc cgtcgctcgt tgatgccatg ttcgcttcga cgatgacatc gccgagcgcc
    12661 gatgtgccag ttaaggtgcc cgagatgctt ccacctcgtg aaccgaagaa tcgaagtgag
    12721 ccgccgccgt cgaaaacgcc atcgttcacg aaattgcctt gaacggtcat cgtccccgac
    12781 gaggcactga ccgtcccggt acttaccgtg aagtccgacg tcgtcgcgtt cgtgaggaac
    12841 gaccagccgc cacccgcgcc actgaatgtc acgtcgccaa gcgaactcgt cgacgtcgta
    12901 ttgatcgttt ttccggtcgt cgtcgcgttg aagaaggtgc ccgatgcatg cgccgtgaag
    12961 agactgctcg aggacgcgat gagattcccc gcgatggtcg tcgtaccgcc cgcgatgtac
    13021 gtcgcgcccg taccgatatg aaacgagccg tcggtcggcg atgccgacgc gttcccgttt
    13081 acgacgacgg gaccacctgg ggcgaaggtc ttccccgctt tcacgtgaag ttcctgtccc
    13141 gcgttcacgg cgagcgttcc gccgttcgcc gtgtactgaa tgtccgggtc gtcatcgccg
    13201 tcgaagaacg agaggtccgc tatcgtggtc gtcgtcgcgc tcgtcgcctc gttcgagacg
    13261 atgacccgat tctggtagag atcgagtccg gaaatggcat cgaccgacga tgtcgccttg
    13321 ctcacgagcg ccgcgcgcat cgccgggtcg ccgtcgaccc acacgacgaa cggcgcgctc
    13381 gcattgagga aaccgaggaa accggcggtg tcgacgctcc atgaaccgtc cgcggcggtc
    13441 gtcgtgctaa gggtggtgtc gagtccattg agtgcccgaa cgctcacggt cttgccggcg
    13501 gacgccacgc tcctgccctc gtcggtgtag agcgtaccgg agagcgtcgg agcgagcgac
    13561 ggcatgtacg tgatgacgag ttgaacgtca tcggcgcgga gttcggaagt tgcggagttg
    13621 ttattacccg tcgcgggcgt gagcgagagg tggagagtca cctggttata cgagaacgcg
    13681 catattccct tcaccggcac ggtaccggtc gcgtttcggg tcgagtaggt tcccgagacg
    13741 gcgcccggat cgaacgacac ctcgagactg ctcgtcgtac acgcgctcac gtctccgctg
    13801 tcattgagcg cgagggccgc agcctgcgtt gcggagacgc tctcctgtgc gacgcgatat
    13861 ttgaagctac cgtcgaccga cgacaccgtc gctcctacgg ggacgcccat gtcctcccac
    13921 gtgagcgact tcgtccaact cacgaccggt gtatcgctct taccgacgca ggtcgacgtg
    13981 aggaccgttc cctgcgcgcc atcggtcgtc gtcgcgttcg ttatcgaggt gtcgcacgcg
    14041 cccgagaccg agctcgagaa cgacgcgtcg gacacggtgt acgttacggt gtcggccgcg
    14101 gctcgaagcg gtgagaatag tacgagaaac gctaaaatga ggaagagccg atggtcgagt
    14161 gggattttat gcggttgtcg gtgctctcga ttcatatgct ttgcccgtta ggcgcctcac
    14221 ctcccacgtc tgccgtcgct tccgacggcc ctttctcggg cggcgcctcg accgatgcgt
    14281 cgacagacgg tgcttctggg atttggctct catcaagatt ctcgccgctc acctcgtccg
    14341 ccgcggccac gtcttccgag gccgaaggac ctgccgccgg ctccgattcg ctcgtcgtgc
    14401 catcatcctc cgcaacggtc ggctccgcgg gcgaactcga agccgtatcg ttcgatgcgc
    14461 tctcctcgac ggcatcagtc gtcgttgccg aatcgcctac atcaccctgc atcgtctcgc
    14521 tttggtctgg tgacgacggc tctgattcgt cggcgttcga gctcgttccg ctctcgcttc
    14581 cgttcgtcga gttgaggagc gcacgaagct cgtccgaggt cacgcagaca tcgtcgacgc
    14641 agagcgtatc gctcacgacg atacggtcgg ctcgtatctc gaggaccgtg agaacgccgt
    14701 ccacgaagtt cgtcgcgagc gtcttgagac gcgaccacag agtctcctcg ctccccggga
    14761 tgggcgccgc gagctcgcgg agtatctccg actccgcgtc tgcgaggtac gtcgtgagct
    14821 cgatgaacat gagggcctgc cctatcgcga cctcctcacc gccttcgagc gtgagcgtct
    14881 ccggtttctc gtccgcgagc tcgcgaagcg cgacgtcgcg gagcgtctct tcgtccatga
    14941 gttccggcgg tatctcacga acgtcgcgcg gcgtacaccc cacctcgccc tccggcgcgc
    15001 cgccgccgta gtaacagccg tcctgtgcgc gcggatcgta cgcgtccttc ttcgctttga
    15061 tggtctcctt caccatgtcg tcgccgaact ggttgaggaa gccgtcggaa tacgcatagc
    15121 ggccgtcgta gtcctcgagc gcgataccga cgacacgcgc gctccccgtc gccttcatac
    15181 cgactccagg caccgacgag agcgtgatgc ggtcgccttt cttgataggg ccgttctcgg
    15241 tcgagagcct gatggggacg cgccccttga ggccgaccgg gtacggcgcc tcgccggtca
    15301 cgagcgaggt gtcgtcgaag ccgagcacga gaccgggttt cgtcgagacg acgccgatga
    15361 cgttcgggga cgatgacgcg tccgccttct cgatggagag gccccccgcg agcgcgacga
    15421 tctctccgga gcggagcgct tccttcgaga agtacatctc ggcgaggtcg gagttgccgg
    15481 tcgcggaggt gaccgaccgt atctgtccgg tcgtcgtcga gacgcacgac ccgtcgttgt
    15541 cgacgcagat gccgccatac ggtatctgga gtcccgcggc gaccgatccg tcgcctatcg
    15601 tcgtcgagcc gttcgccgcg atgatgaact tcgaggtacc cgcgacctta aggtcgatga
    15661 acttagccgt cgacgtcgcc aggaaggaac caccggcgtt cccgaagttc atctcgaggc
    15721 cggtccccgt gaactcgctc gtgtcctgga agagggacac gagggcctcc gaggtgatgg
    15781 tccccgagta cgcgcgaaga gcgttcccct gcgctgtgcc ctgcgtctcc gcgaagacgc
    15841 cggccggaag gtacacgctt cccgcgtcgc cgagggtcgt accccggacg ccgaacgtac
    15901 ggccgtagcc cgagataccg gtattctctc cgttcgtgtt cgtaccgcgg aaggcctgcg
    15961 cctcgaagcc gcgcaccgtg ttccccaagg tgctcgtgtc cgcgatccga atcatgctcc
    16021 cgacgagggt cgacgtcgcc gtcgggttcg tcgtgatgta cgtctcgttc ccgtacgaga
    16081 ccgcgctcgc gaccgcgttc acgaaaccga agctttgctt gatgccgccg acgaccgagc
    16141 cggagagaga ctgtacgtcc atggtgagga gcgaggtcgg cgtcgtcgtc ccgatgccga
    16201 cgttgccgtt cccgtcgatg cgcatgcgct ccgcgctgtt caccctaaag gagagccgct
    16261 gggaggtcgg cgtgccggag ttcacgccgt acgagatgga gccctgctcg gggagcgcgg
    16321 tgttcccgaa gtagatggtc gagctcgcgc tcgagacggt gaagaagttc accgcgacgc
    16381 cacttcccgg gaacgtctcg ttatcgaacg acgcgaccga ttccgtggag aggaagttcg
    16441 gagacccgat gcccttcttc accgtgagcg tggcacccgt gccggcgaga agcgtggcgc
    16501 cgtctcgaac cgaaagacga tacccgttaa ccgccgcccc gccgagactc acctgtccgt
    16561 tcgcgagaac cttgaggata ttgctcgtcg tactcgtcgc gaggatgaag aggggcgagg
    16621 tccccgtaag gctcgtcgat gcgacggtga ggatggcggt cggcgtcgag gagccgatgc
    16681 cgacgttgcc tccgaagtac gccgtgccgg tcgtcgtcgc gccaccgttt atggtgaggc
    16741 cgcccgcctg cgtaccgtca ccgatcgtcg aggaggcatg gacggagaac ccgacggtcg
    16801 acgacggtgt gagcgcacca tttgagacca cccagttgct cgacccactt cctcccgtga
    16861 taccgagcgt cgaggtcgcg acccacgcga cgccgctccc aacgctctgg agcaccatac
    16921 cgcttgcgcc cggattatac gtcccgtcgt agagcgcgcc ggttagatgc atgttccccg
    16981 cgacggagag aagctccgcg ggcgtcgtcg tacccaagcc aagccgcccc gcgttcgtca
    17041 cggtgagaac gcgagtcgtc gcattgtaga ggtcgagtat cgagccggtc cccttttgcg
    17101 ccacctcgac ggtcgacgat gaagacgagg cgtttatggt gaatatgctg tacgcgcgcg
    17161 aagttggcgt accgagggtg acgcggtcat atgcgtcgta ccgcgtgtcg gtgccaccgt
    17221 cgctcacgta cgtcgtcgtg tccgggttca cgtacgtgtt gttcgcgatg gtattccccg
    17281 tccccccctg gatctgtatg agagggtcgg tcgacgtcgc ctgaatggtg ttcccggtga
    17341 tgaggtcgta gttatcgttc acgtagatgg tcgcgtacgc accgccgttc gtattgccat
    17401 agaagacgtt ccccgtgatg acggtaccac tcccggaatc gctcacgatg gcgtactgcg
    17461 aacttccgat gaacgtgttc cccgatatcg tgacgttcgg actcgcgctg aactgtattg
    17521 cacgcgtatt acccttaaaa ctattgccag tgatgacgtt atttcccgag gtgctgttcc
    17581 cgaagacgcc atagagtcca ttctcggtga acgtgttccc cgtgatgacg ttgtactgcg
    17641 acgatgcggt gaggtataca cctgcctgcg tgtctcccgt gaacgagttg ttctcgatcc
    17701 tgctgtacgt actgccggcg tagataccgt atccgcgaag acgcctgaag gacacgttct
    17761 cgaccgacgc atacgaactg ccgagcacga tgccgtactg ggtcgaccct accgccgcag
    17821 aaccatctat cgtgaggtcg cggaccgtca cccccacctt ccccgacgac gcgataacgc
    17881 cccagttggt cgatgtcgcg gtaagggcaa ggagcgtgtt cgcgcctcct cccatgatgg
    17941 tcgtcgacga ttggagcacg tttatcgaat tgttgatcga gtacgtgccc ggaaggaggt
    18001 aaacgagacc gcccgtagac ggcaaagccg aaagtgcggc gtttatcgtg tcctggtcgt
    18061 tcgtgcccgt cgcgacgaag tcggcgctct ccttgttcgt cgtgtccgag gcggcgacga
    18121 ggtacgtcgc ggtcgagcgg tcggtctgcc acttcccgtt ctcatacaca tagagcgtcg
    18181 aggaagcggt cgagaagaag agcgtgccct ccgaaggact cgtcgggaag gatgtccctc
    18241 ccgtaatgcg agcggagccg gcgataagcg ccgaggtcgt cgccgtaccg gagacggaga
    18301 gatcggagag gaacgccgtg ccatctacgg taagcgccgt ccccgaggcg ttcgcgaacg
    18361 tgaggagccc cgagagggtc ggtgatgccg agaacaccac tctccccgcg gtccccgtct
    18421 catcggtgag gagcgccgcg agctctgcgg aggtgtcgat cgaggtcgag
//
LOCUS       DASTSP010000010        14792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1367977, whole genome shotgun sequence.
ACCESSION   DASTSP010000010 DASTSP010000000
VERSION     DASTSP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367977"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFS75_00375"
     CDS             <1..227
                     /locus_tag="VFS75_00375"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU4677158.1"
                     /translation="PNHPRYSMLTHIDAYRIESDDEMRVLGFKDIVEDASRLVAIEWP
                     EKIATIMPAYALPVALTIVDGTTREITYDT"
     gene            217..2610
                     /locus_tag="VFS75_00380"
     CDS             217..2610
                     /locus_tag="VFS75_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEU4677159.1"
                     /translation="MTRKKTTTEEKRRTLVLLDAHAILHRAYHALPDFSSPKGEPTGA
                     LYGVVAMLLKIVTELKPDYLVACYDLPEPTYRHDVFKDYKAGRAELEDGLIAQIERSR
                     DIFKAFGVPIYERAGFEADDILGTIVEEVKGVKDLRTIIASGDMDTLQLVDDDHVLVY
                     TLKKGINDTILYDEKAVFARFGFGPTQVPDFKGFRGDPSDNIPGIKGIGEKTATELIV
                     NFKSVEGVYKALKKDETLLKKAGIKDRMIELVRANEEEAIFSKMLATIRRDAPIDFKL
                     PETTWRDGVDTELVLTLFAELGFRTLGARVEQIFHVEASDRAEEVGGLSQEEVEELGL
                     ALWLLSSDTTNPDEGDIMQYGRANELRTVPLIRKRLMEELKKENLERVYEDIELPLRG
                     VLHEMERTGVRIDVAHLTRLSKRMHAEIEKLEARMYELAGTEFNVNSPKQLGDVLYDK
                     IGLAPKKQKHTATGQRSTRESELEKMRDQHPIIADILRYREVQKLASTYVDVIPTMVG
                     EDGRVHTTFLQAGSVTGRMASQNPGVQNIPVRTEEGRLIRKAFVADPGFKLVSIDYSQ
                     VELRIAAILSGDTKLIEIFQKGEDVHQGVASQVFNVPFSEVTPNMRRQAKVINFGILY
                     GMGVNALRQNLGETTTRAEAQEFYNAYFNTFTRLAEYLEETKAFARAHGYTVTHFGRK
                     RRFPGMNSSAQFIRAQAERMAINAPVQGTAADVIRIAMVRIAEYLKREKLTEDVRMLL
                     QVHDELVFEIREERVKELVPELKRIMETVLTGDEAKGVPIIAEAKVGDNWDEMEKMK"
     gene            2612..3313
                     /locus_tag="VFS75_00385"
     CDS             2612..3313
                     /locus_tag="VFS75_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677160.1"
                     /translation="MLYAYFGEDTAAVRNEAFRALRRMEKGALVTTVTADTWNAAALS
                     DLAGGASLFGEAQVIVLDTISEGGADAKAELFERLDVLKGSSHNFILIEGSLKADEKK
                     RLAKVAEEAKEMEGEKAERFNAFAMTDALLRKDKKSLWLLLTAAWREGLSNEEIIGVL
                     FWQLKTLRLVAKAKDATDAGLKPFVYGKAKRALSSFTEKELDRLSLSLLSAYHDGHAG
                     RADIATALEAWVLSL"
     gene            3352..3424
                     /locus_tag="VFS75_00390"
     tRNA            3352..3424
                     /locus_tag="VFS75_00390"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3385..3387,aa:Lys,seq:ttt)
     gene            complement(3783..4265)
                     /locus_tag="VFS75_00395"
     CDS             complement(3783..4265)
                     /locus_tag="VFS75_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HEU4677161.1"
                     /translation="MNDSVPMEHERRFVPDLAAVPMKFSKCPNSDIVQAYLRDGETRI
                     RMETENGSRTYTKTVKRGEGISREEHEVSVTEAEFDLLWDDVEAQLSKRRYRIPWDGK
                     TFELNIFDGPLTGYVQFEIEFPSEEEARSYKPPHWVGREVTDDKRHTNASLAKYGIPR
                     "
     gene            complement(4319..5125)
                     /locus_tag="VFS75_00400"
     CDS             complement(4319..5125)
                     /locus_tag="VFS75_00400"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEU4677162.1"
                     /translation="MTYDIPHLLLHGTLAPSPAFLALFTLASALLTLLAVSCYLHRSQ
                     THRAIRFIAPLNHVFRLILWFTTGMVTKEWVAIHRKHHAMCDTSEDPHSPRYFGVRKI
                     LLEGADIYRKAGTEHPEILVEYGEGTPDDWLERHVYAGALRNWGIRLTLIVELILFGL
                     PALIIWPIQMLVIPIIAAGFINGIAHVWGYQRYKDGMPRGDGKVYERIGDARNVPTYG
                     LAVGEEFHNNHHAEQWNYKFSHAWYEFDLAGTFAELLTWIGFAWVPRRRN"
     gene            complement(5127..5513)
                     /locus_tag="VFS75_00405"
     CDS             complement(5127..5513)
                     /locus_tag="VFS75_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677163.1"
                     /translation="MLNPFPIQFLSLFAYAALRLVVAIVFLKEMSAHVRRREAVEAKM
                     PFMRAFLSRALPVFEFIVAAMFVAGFLTQVAALLGGAYALKGVLLAGRYPTVFSRGRL
                     FYFLLFGAAASLFVTGAGYLAVDLPL"
     gene            5552..6016
                     /locus_tag="VFS75_00410"
     CDS             5552..6016
                     /locus_tag="VFS75_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU4677164.1"
                     /translation="MRTYVILHNIRSAHNVGSVFRTADGAGVAKLYLTGYTPGPIDRF
                     GRPHAEIEKTSLGATKTVPYEAGREVNEVLDELTAAGVELVAVEQTAQALDYRTFRPS
                     RDTAFIFGNEVDGIEESVLERASVHIEIPLEGRKESLNVSVCAAVILFTMRP"
     gene            complement(6013..6984)
                     /locus_tag="VFS75_00415"
     CDS             complement(6013..6984)
                     /locus_tag="VFS75_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677165.1"
                     /translation="MKDLAGIIGFIIILMVFSVYTEGHPITGDDSTPLFSFGVDDTST
                     PAPSYEDETIEASDDSYAVTRLSPREIEERLAELEDELDAAKKDAYEARLRQPASPYQ
                     GLVTLGRGDSGETSLRREYVTLQTAYDSPQRVDVTGWTLESYITGERAVIPKGDRIVE
                     DPKDPSFEHIVLYPGETAYVITGRSPIGTSFHENQCTGYLDEDYSVYPALDRMCPDAT
                     KEMERWSTVTVTDDECYDFMRSVYSCEIPDEDVVDDAHLSSACRRFVLNELTYDSCVQ
                     HHALDPLFDDVGYWYVYLGRGHELWRVEREVIRLLDGNGKVVDVVEY"
     gene            complement(6981..8273)
                     /locus_tag="VFS75_00420"
     CDS             complement(6981..8273)
                     /locus_tag="VFS75_00420"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU4677166.1"
                     /translation="MDNFLYYVKRLIPTSLLRLLQPPYHYLLAVIGALWYRFPSKEIS
                     VIGVTGTKGKSTVVELINAIFEADGRRTALAGTIRFKLGEKSERNLYKMTMPGRFFTQ
                     RFLRDAVNAGCDVAILEMTSQGVLQYRHKFIHLDALVFTNLSPEHIEAHGSYEKYIAA
                     KLELATALAESKKRPRFIVANRDDEQGGAFLATDVEHKLPYGLSDLELHTLNKDDVSL
                     VLEGTTIRVPLVGLFNVYNALAAITVTRAFGVPMKTITRALTELSPIKGRVERFRSPK
                     GAEKKVTAIVDYAHTPDSLEKLYQAFEKERKVCVLGNTGGGRDTWKRPEMGRIAEKYC
                     ERVILTNEDPYDEDPRKIVDEMARGIDDASKLSIIMDRRKAIRTALEEAPDGSVVIIS
                     GKGTDPYIMGPHNTKMPWSDAKVVTQELASLFGDSAAS"
     gene            complement(8331..8900)
                     /gene="ruvA"
                     /locus_tag="VFS75_00425"
     CDS             complement(8331..8900)
                     /gene="ruvA"
                     /locus_tag="VFS75_00425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU4677167.1"
                     /translation="MIATLTGQVTDIGAAHIVLDVGGVGYLVYVTGSSGFTIGESATL
                     FTHLAVRENALDLYGFKTRDERTMFEHLIKLPKIGPKTASQILAQVDLPTLRRAVASE
                     DPSYLSKMSGIGKKSAEKIVSGLKDILGDEALADFPAHEPNVDTDVIEALLALGYSER
                     DARDAAQRIPADIAGTNARIKHALREVKR"
     gene            9030..12035
                     /locus_tag="VFS75_00430"
     CDS             9030..12035
                     /locus_tag="VFS75_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEU4677168.1"
                     /translation="MSHFETIARGKHFARTFGLFLVALTVFIGSFVLFLSPVEAGSAS
                     FGFTYIPKAGQPIPKDSNVSMTVYTNKDTFSPGESGTIYVDISGENPQPSYGMGITYR
                     VNSGSATNWNQTSSNVAIRAGHTYTVGTFTAPSTGGSFTLNIQASLSDYTGPYGKSIT
                     LNVQAPNQTLTTRVVGSGSISSNPGGISCGGDCSESYAYGTNVSLSATPSSGYVFTGW
                     SGDCSGTGSCSVSMTSSRNVTATFVASCTVTTDSSWSACSTSCGPGTQYKYYRTSPGS
                     TCGGYYNGQVADSRSCNLGACPVPPTASLTVSPNVVQYGGTTQVSWSSTNADTCYFYW
                     DGSPWVTKGSGGSFMTNALNMAGYHSITMQCKNTGNGLTSNWQTGSVTVIPPSPSGLT
                     GSCNGLTAQASWNGVAGASSYAFRSDNTTIGSWNGTCDPSTGAGDICETTTATSRSWS
                     AGYGYNYSVWVHSCSAVGCSATPSSISFSCPAAPQPDLVSEAILVPASGTVGTPLDLA
                     ATVRNAGGAGTGVAFKDDFSYRWGTTGTWTSLGLLPESALASGASANDTDTYTPTQTG
                     TLYIQHCVDSTNVVVEGANETPNCSVAPAITVSASSSAPTVVTISDTYAPGDTAVTLN
                     GTANPNGSDTIARFRWGTTDPLGDCAALPNLTGNAMVGNGTTSVNYAGLLPGVTTGTT
                     YYYCAYGASAAGVGYGAVRSFGAGGSAGIDLRSENLSVPSSGKVGSALNFSAMVHNVG
                     ATAAGAFKDNFSYQWDGTSGSWTDINPFTNHASLAAGATSNDNASYVPSRTGTLYIQH
                     CVDSANDVDEGANETPNCTIGDSDASDGGGGGGGGGGGGGGGGGGIPIVDGEIDGDPT
                     IVGSNGTSTITWSTNGAGNCTVSGGADSWSGTSGSQVTSPLNADTTYTLVCDGVLIDS
                     VTVQVRTTPELVASPRVVNQGSQTTLTWRLNGGVNCTLTGGGLNLGGLNGNSSVNVTV
                     SANTTYTLTCPGRGSASITVEVIPVGFET"
     gene            12052..12387
                     /locus_tag="VFS75_00435"
     CDS             12052..12387
                     /locus_tag="VFS75_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677169.1"
                     /translation="MPEQRNTENERRDEKRVQILAVLAVLILVTIALVMLVRERQERM
                     NAADVPVSTAANEAVSVPTASANAASIYPANVRTAFDEIRRKVAEGEMTPQDADTATA
                     KLMDDVPTP"
     gene            12404..12949
                     /locus_tag="VFS75_00440"
     CDS             12404..12949
                     /locus_tag="VFS75_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677170.1"
                     /translation="MTRHSANVLASIVLIGVLIVAIGTTLWRRTHMNVPSVDVAKDEP
                     AAEPIREQWTGVVQSVSADSLTLTVVVPEGQGDRTVSLTNDTIFKKATLRDPDTVKRE
                     MAAFEKAMEAYRKAYDASSTVSSTPPPVLPLAATEETIAPADIPIGASVSVVATVPGS
                     VNGAAVAESVEVIPPAPSTSE"
     gene            13037..>14792
                     /locus_tag="VFS75_00445"
     CDS             13037..>14792
                     /locus_tag="VFS75_00445"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677171.1"
                     /translation="MRLTHQFLDARFTRQGAILLAVVAALVLSYTVSPTRVEAGSASF
                     NLNCVSNCSTDPDVLATISTNKNTYSPGEVGSLTIKYESDAPAGSSFTGNATWTGGGS
                     WSGSVSRLQITTGATISIGSFTVPSTGSSYTLTVNGSLSPNYNGAASQNIVVSISQPN
                     QTLTTSVSGGGTITSSPAGINCGATCSASYAYGTNVSLTATPSSGNTFTGWSGACSGT
                     GGCTVSMTQARNVTASFGAACTVTHDTSWSACTASCGGGWQYEYYHTSPGSTCGAISN
                     GQVYAQQQCNTQACPVGPSATMWVSPSPIAYGGSTVYGWSTSNATYCRFYVDGNLAGD
                     TNNGWPINNASGWADAPLYTDRTYRIECTNATGQWTSSQATVDITPLIVLDSVTSGQV
                     GSCGFVSGWAFDPSRPSQSINVHQYYYNPFTGYDTGPTAVNRADVNSTYGITGVHGFQ
                     YQIPIAAAGTYTARSYAIGINGSGVQNGNNPFATEKTFTCDPAPVNNASCVSVTGVPA
                     SIYAGQTFTPTVTTYNNGTKQWNRDGTPHSIGFATPYDSLLFGLNRVSINPTTVNPGQ
                     YSTMTFTATAPTSAGTYQF"
BASE COUNT         2931 a         4463 c         4672 g         2726 t
ORIGIN      
        1 taccgaatca cccgcggtat tcgatgctca cgcacatcga cgcgtaccgc atcgaatcgg
       61 acgacgagat gcgcgttctc gggtttaagg atatcgtcga agacgcgtcg cgcctcgtcg
      121 cgatcgagtg gcccgagaag atagcgacca tcatgcccgc ctacgcgctc cccgtcgcgc
      181 tcacgatcgt ggacgggacg acgcgcgaga taacgtatga cacgtaagaa aacgacaacc
      241 gaagagaagc gaaggacgct cgtcctcctc gacgcgcacg cgatactcca ccgtgcgtac
      301 cacgcgcttc cggacttctc gtcgccgaag ggggagccga ccggtgccct ctacggcgtc
      361 gtcgcgatgc tcctcaagat cgtgaccgaa cttaaacccg actacctcgt cgcgtgctac
      421 gaccttcccg aaccgacgta ccggcacgac gtcttcaagg actacaaggc gggccgcgcc
      481 gagctcgagg acgggctcat cgctcagatc gagcgctcgc gcgacatctt caaagcgttc
      541 ggtgtgccca tctacgagcg cgccggattc gaggcggacg acattctcgg tacgatcgtc
      601 gaggaggtga agggagtgaa ggacctccgc accatcatcg cctcgggcga catggatacg
      661 ctccagctcg tcgacgatga ccacgttctc gtctatacgc tcaaaaaggg catcaacgac
      721 accattctct acgacgagaa ggcggtcttc gcgcgtttcg ggttcggtcc gacgcaggtc
      781 cccgacttca aggggtttcg cggcgacccc tcggacaata tccccggcat aaaaggcatc
      841 ggcgagaaga cggcgaccga gctcatcgtc aatttcaaat cggtcgaggg cgtctacaag
      901 gctctcaaga aggacgagac tctcctcaag aaggcgggca tcaaggaccg catgatcgag
      961 ctcgtcagag cgaacgagga agaggcgatc ttctcgaaga tgctcgcgac gatacgccgc
     1021 gacgcgccga tagacttcaa gctccccgag acgacgtggc gggacggcgt cgacacggag
     1081 ctcgtcctca cgctcttcgc cgaactcggc ttccgaacct tgggcgcgcg cgtcgagcag
     1141 atattccacg tcgaggcgag cgatcgagcg gaagaggtcg gaggactctc gcaagaagag
     1201 gtcgaggagc tcggcctcgc gctctggctc ctctcgtcgg acacgacgaa ccccgacgag
     1261 ggcgacatca tgcagtacgg ccgcgcgaac gaactccgca ccgtccccct tatccggaag
     1321 cgtctcatgg aggaacttaa gaaggagaac ctcgagcgcg tgtacgagga catcgagctt
     1381 ccgcttcgcg gcgtccttca cgagatggag cgaacgggcg tgcgcatcga cgtcgcgcac
     1441 ctcacgcgcc tctcgaagcg catgcacgcg gagatcgaga agctcgaggc gcgcatgtac
     1501 gagctcgcgg ggacggagtt caacgtgaac tcgccgaagc aactcggcga cgtgctctac
     1561 gacaagatcg gcctcgcgcc gaagaagcag aagcacacgg cgacgggcca gcgctcgacg
     1621 cgtgaatcgg agctcgagaa gatgcgcgac cagcatccga tcatcgcgga cattctccgg
     1681 taccgcgagg tgcagaagct cgcctcgacg tacgtcgacg tcatcccgac gatggtcggg
     1741 gaggacgggc gcgtccacac gacgttcctc caggcaggct cggtgaccgg ccgcatggcc
     1801 tcgcagaacc cgggcgtcca gaacattccc gtgaggaccg aggaggggcg cctcattcga
     1861 aaggcgttcg tcgcagaccc cgggttcaag ctcgtttcca tagactactc tcaggtcgaa
     1921 ctccgcatcg ccgccatcct ctcgggtgat acgaagctca tcgagatatt ccaaaaggga
     1981 gaggacgttc accagggcgt cgcgtcgcag gtctttaacg ttccgtttag cgaggtgacg
     2041 ccgaacatgc gtcggcaggc gaaggtcatc aacttcggta tcctctacgg catgggcgtg
     2101 aacgcgctcc gccagaacct cggcgagacg acgacgcgcg ccgaggcgca ggagttctac
     2161 aacgcgtact tcaacacgtt cacgcggctc gccgagtacc ttgaggagac gaaggcgttc
     2221 gctcgcgcgc acgggtacac ggtgacccac ttcggacgga agcgccgttt cccgggcatg
     2281 aactcctcgg cgcagttcat ccgcgcgcag gccgaacgca tggcgatcaa cgcgccggtt
     2341 caggggacgg ctgccgacgt cattcgtatc gcgatggtcc gtatcgccga gtacctaaag
     2401 cgagagaagc tcacagagga cgttcgcatg ctcctccagg tccacgacga gctcgtcttc
     2461 gagatacgag aggaacgcgt gaaggagctc gtccccgagc ttaaacgcat catggagacc
     2521 gtgctcacgg gggacgaagc gaaaggtgtc ccgatcatcg ccgaggcgaa ggtcggcgat
     2581 aattgggacg aaatggagaa gatgaaataa catgctgtac gcgtacttcg gtgaggatac
     2641 ggcggcggta cggaacgagg cctttcgcgc gctccggcgg atggagaagg gcgcgctcgt
     2701 tacgacggtg acggcggata cgtggaacgc cgctgcgctc tccgaccttg cgggcggtgc
     2761 atcgctcttc ggcgaggcgc aggtgatcgt cctcgacacc atctccgagg gcggcgccga
     2821 cgcgaaggcc gagctcttcg agcgcctcga cgtcctcaag ggctcttcgc acaacttcat
     2881 tctcatagag gggagtttga aggccgatga gaagaagcgt ctcgcgaagg tcgccgaaga
     2941 ggcgaaggag atggaaggag agaaggcgga acgcttcaac gccttcgcga tgactgacgc
     3001 gctcctccga aaggacaaga agtccctctg gcttctcctc acggccgcat ggcgagaagg
     3061 actctcgaac gaggagatca tcggcgtcct cttctggcag ctcaaaacgc tccgcctcgt
     3121 agcgaaggcg aaggacgcta cggatgcggg gctgaagccg ttcgtctacg gcaaggccaa
     3181 gcgcgcactg tcgtccttca ccgagaagga actcgaccgc ctttcactct cgctcctctc
     3241 cgcctaccac gatggacacg cgggccgcgc cgacatcgcg accgcgctcg aggcatgggt
     3301 gctctccttg tgatgacaag agagttccat gtgctatagt cctcacgata tccgcccata
     3361 gctcagatgg tagagcagct cccttttaag gagacggtcc caggttcgat ccctggtggg
     3421 cggacagatt tcgaagaaat atggacgcac aaagacactg tctgggagac agtgtcttaa
     3481 gggatcgaag gccttgagca ggtactttgc gaagaaatgc agcaaagtac gcgaaaggcg
     3541 tactgaacat gtaatgttcg atccctggtg ggcggacaag cgttaagaaa tcctggcctg
     3601 cgccaggatt ttagcttgtc cgagccggag cgatgttttg cgaatgcaaa accgcgaggc
     3661 ggggtcgcga gtccttagcg agtgaagcga gcttaggaac tcgcgacgat atttttcgag
     3721 ggtggacagt cgagactaaa aaagaaaccc gccgtgaggc gggtttgtac gtttgggaac
     3781 attcatcgcg ggatgccgta cttcgcgagc gacgcgttcg tgtgccgctt gtcgtcggtc
     3841 acctcgcggc cgacccagtg aggcggcttg tatgaccgcg cttcttcctc agacgggaac
     3901 tcgatctcga actgcacgta tccggtgagc ggtccatcga agatattgag ctcgaaggtc
     3961 tttccgtccc aggggatgcg gtagcggcgt ttcgaaagct gcgcctccac atcgtcccag
     4021 aggaggtcga actccgcttc ggtgaccgac acctcgtgct cttcgcggga gatcccttcg
     4081 ccgcgtttca cggtcttcgt gtacgtgcgg ctcccgttct ccgtctccat gcggatgcgg
     4141 gtttctccat ctcggagata cgcctgcacg atgtccgagt tcgggcactt cgagaacttc
     4201 atgggaacgg cagcgaggtc cggcacgaac cgccgttcgt gttccatcgg tacggaatcg
     4261 ttcatgagcg tgtcctcata aaagtctgcg ctcgatagta caggaaggcg agcgcgtgtc
     4321 aattacggcg ccgaggcacc cacgcgaagc ctatccacgt gaggagttcc gcgaacgtgc
     4381 cggcgaggtc gaactcgtac catgcgtgcg agaacttgta gttccattgc tcggcgtgat
     4441 ggttattatg aaactcttcg ccgaccgcga ggccgtacgt cggtacgttc cgcgcgtcgc
     4501 cgatccgctc gtacaccttg ccgtcgccgc gcggcatgcc gtccttgtat cgttggtacc
     4561 cccagacgtg cgcgatgccg tttatgaatc ccgccgcgat gatggggatg acgagcatct
     4621 ggattggcca gatgatgagg gcggggaggc caaagagaat gagctcgact atgagcgtga
     4681 gtctgatgcc ccagttccgg agggctccgg cgtagacgtg ccgttcgagc cagtcgtccg
     4741 gcgtgccctc tccgtactcg acaaggatct ccggatgttc cgtaccggcc tttcggtaga
     4801 tgtccgcgcc ttcgagaagt atctttcgaa cgccgaagta gcgggggctg tgcgggtcct
     4861 cggaagtgtc gcacatcgcg tggtgcttcc tatgtatcgc gacccactcc ttcgtcacca
     4921 tgccggtcgt gaaccagagg atgagccgga agacgtggtt aagcggcgcg atgaaacgga
     4981 tcgctcggtg cgtctgtgac ctatgtaagt agcaggaaac cgcaaggagc gtgaggagcg
     5041 ccgaggcgag cgtgaagagc gcgaggaagg cgggtgatgg cgcgagggtg ccgtggagga
     5101 ggaggtgtgg gatgtcgtag gtcatgttag agcgggaggt cgacggcgag gtaaccggcg
     5161 ccggtcacga aaagagatgc ggccgcgcca aagaggagga agtagaagag gcggccgcga
     5221 gagaagacgg tcgggtagcg ccctgcgagg aggacgcctt tgagcgcata ggcaccgcca
     5281 agaagtgcgg cgacctgtgt gagaaatccc gcgacgaaca tcgccgcgac gatgaactcg
     5341 aacaccggga gcgcgcggga gagaaatgcg cgcatgaagg gcattttggc ctcgaccgcc
     5401 tctcttcgtc gtacgtgcgc gctcatttcc ttgaggaaca cgatggcgac gacgaggcgg
     5461 agcgcggcgt aggcgaagag cgagagaaac tgtatgggaa acggattcag catggcgtat
     5521 cgcgtgcccc tagtgtagca tgcgagacag tatgagaacg tacgtcatcc tccacaacat
     5581 acggagcgcg cacaacgttg gctccgtgtt ccgcaccgcc gatggcgccg gcgttgcgaa
     5641 gctctacctc accgggtata cgcccggtcc cattgaccgg ttcggccggc cgcacgcgga
     5701 gatagagaag acgtcactcg gcgctacgaa gaccgtgccg tatgaggcgg ggagggaggt
     5761 gaacgaggtc ctcgatgagc tcaccgccgc gggcgtcgaa ctcgtcgcgg tcgagcagac
     5821 ggcgcaggcg ctcgattacc ggacgttcag gccgagccgt gacacggcgt tcatcttcgg
     5881 gaacgaggtc gatggcatcg aggagagcgt ccttgagcgc gcgagcgtcc acatcgaaat
     5941 tccgctcgag ggcaggaagg agtctctgaa cgtctcggtg tgcgccgccg tcattctctt
     6001 caccatgcgc ccctagtact cgacgacgtc gacgaccttt ccgttcccgt cgaggaggcg
     6061 tatgacctcg cgctcgacgc gccagagctc gtgtccgcgg ccgaggtaca cgtaccagta
     6121 gccgacatca tcgaagaggg gatcgagcgc gtggtgctgt acgcaactgt cgtacgtgag
     6181 ttcgttgagg acgaaacgcc ggcacgcgga ggagagatgc gcgtcgtcga cgacgtcctc
     6241 gtcgggtatc tcgcacgaat agacgctccg catgaagtcg tagcactcgt cgtcggtcac
     6301 cgtgaccgtg ctccagcgct ccatttcctt cgtcgcgtcg gggcacatcc gatccagggc
     6361 ggggtacacg ctgtagtctt cgtcgaggta gccggtacat tggttctcgt gaaaggaggt
     6421 gccgatgggc gagcggccgg tgatgacgta cgccgtctct cccggataga gcacgatgtg
     6481 ctcgaaggac gggtccttcg ggtcctcgac gatgcgatcg cccttgggga tgaccgcgcg
     6541 ttcgccggta atgtaactct cgagcgtcca ccccgtcacg tcgacgcgct ggggagagtc
     6601 atacgcggtt tgaagcgtta cgtactcgcg gcgcaggctc gtttcccctg aatcgccgcg
     6661 gccgagcgtg acgagtcctt ggtacgggga cgccggctgt cgaaggcgcg cctcgtaggc
     6721 gtctttcttc gccgcgtcga gctcgtcttc gagttcggcg agccgctcct cgatttcgcg
     6781 cggtgagagg cgcgtcacgg cgtacgaatc gtcggacgcc tcgatcgtct catcctcgta
     6841 cgacggcgcg ggcgtactcg tatcgtcgac gccgaaggag aaaagcggtg tggaatcgtc
     6901 accggttatc gggtggcctt ccgtgtacac gctgaagacc atgaggatga tgatgaagcc
     6961 gatgatgccc gcgaggtctt tcatgatgcg gcgctgtcgc cgaagaggga ggcgagctcc
     7021 tgggtcacga ccttcgcgtc gctccacggc atcttcgtgt tatgcggtcc catgatatac
     7081 gggtcggtgc cttttccgga gatgatgacg accgagccgt cgggtgcttc ctcgagcgcg
     7141 gtacgtatcg cctttcgccg gtccatgatg atcgacagtt tcgacgcatc gtcgataccg
     7201 cgcgccatct cgtcgacgat ctttcgcggg tcctcgtcgt acggatcctc gttcgtgagg
     7261 atgacgcgct cgcagtactt ctcggcgatg cggcccatct cggggcgttt ccacgtgtcg
     7321 cggccgccgc ccgtgttccc gagcacgcag accttccgct ccttctcgaa cgcctggtag
     7381 agcttctcga gtgagtccgg cgtgtgcgcg tagtcgacga tcgccgtcac cttcttttcg
     7441 gcgcccttgg gcgacctgaa gcgctcgacg cgccctttga tgggggagag ttcggtgagc
     7501 gcgcgcgtga tggtcttcat cggcacgccg aacgcgcggg tcaccgtgat cgcggcgagc
     7561 gcgttgtaca cgttgaagag cccgacgagg gggacgcgga tggtcgtgcc ctcgagaacg
     7621 aggcttacgt cgtccttgtt gagcgtgtgg agttcgaggt cggagagacc gtacgggagc
     7681 ttgtgctcga cgtcggtcgc gaggaacgcg ccgccttgct catcgtcgcg gttcgcgacg
     7741 atgaagcgcg gtcgtttctt cgactccgcg agagccgtcg cgagctctag tttcgcggcg
     7801 atgtactttt cataggagcc gtgcgcctcg atgtgctccg gcgagaggtt cgtgaagacg
     7861 agggcgtcga ggtggatgaa cttatggcgg tactggagga cgccctgcga ggtcatctcg
     7921 aggatggcga cgtcgcagcc tgcgttcacc gcgtcgcgga ggaagcgctg tgtgaagaag
     7981 cgcccgggca tcgtcatctt gtagaggttc cgctcgctct tctcgccgag cttgaagcgg
     8041 atggttcccg cgagcgcggt gcgtcggccg tccgcctcga agatcgcgtt tatgagctcg
     8101 acgaccgtcg acttgccctt cgtgccggtc acgccgatca ccgatatctc cttcgagggg
     8161 aagcgatacc agagggcgcc gatgaccgcg aggaggtaat ggtagggcgg ctggaggagt
     8221 cgaaggaggg aggtcggtat gaggcgcttc acgtagtaga ggaaattgtc cataggcgaa
     8281 tatcgaaagt atacccgttt ttcggggttc ggcgaggcgg gacgaatgga ctagcgcttc
     8341 acctcgcgga gcgcgtgctt gatgcgcgcg ttcgtccccg cgatgtccgc gggtatccgc
     8401 tgggcggcat ctcgcgcgtc acgctcggag tagccgaggg cgaggagcgc ctcgatgacg
     8461 tccgtgtcga cgttcggttc gtgcgcgggg aagtcggcga gcgcttcgtc gccgaggatg
     8521 tccttcagtc ccgagactat cttctccgcg ctcttcttcc cgatgccgga catcttcgag
     8581 aggtagctcg ggtcctcgga ggcgacggcg cgccggaggg tcggaaggtc cacctgcgcg
     8641 aggatctggc tcgcggtctt cggccctatc ttcgggagct tgatgaggtg ttcgaacata
     8701 gtgcgttcgt ctctcgtctt gaatccatag aggtcgagcg cgttctcgcg gacggcgagg
     8761 tgcgtaaaga gggtggcgct ctcgcctatg gtgaagccgc ttgaccccgt cacatagacg
     8821 aggtacccga ccccgcccac gtcgagaacg atatgcgcgg cgcctatgtc ggtcacctgt
     8881 ccggtgagag tcgctatcat agggaaatgc taccgttaaa cgctccgtcg cacaatcgcg
     8941 tacatatata ttgtgcataa gtagtttcga agtacggccc gcttgataca cttgaggtaa
     9001 gcaagcggcc tcgctggccg cgttcgcaca tgtcgcattt tgaaaccatc gcacgaggga
     9061 agcactttgc acggacattc ggcctttttc tcgtcgcatt gaccgtgttc attggctcgt
     9121 tcgtgctctt cctttctccg gtcgaggccg ggagcgcatc gttcggattc acgtatatac
     9181 cgaaggccgg gcaaccaatc ccgaaagact cgaacgtgag tatgacggta tacacgaaca
     9241 aagacacgtt ctctccgggt gaatcgggca cgatatacgt cgatatctcc ggcgagaacc
     9301 ctcagccgtc ctatggaatg ggtatcacgt accgcgttaa ttcggggagc gcgaccaact
     9361 ggaaccagac ctcatcgaac gtcgccatcc gagcaggcca cacatatacc gtcggtactt
     9421 ttaccgcccc gtcgacgggc gggtcgttca cgctcaatat acaggcgagc ttaagtgact
     9481 atacggggcc gtatggcaag agtatcactc tgaacgttca ggcgccgaac caaacgctca
     9541 cgacgcgggt cgtcggttcg ggctccatct cgtcgaaccc aggcggcatc agctgtggtg
     9601 gagactgtag cgagtcgtat gcgtacggga cgaacgtgtc cctctcggcg acgccgtcct
     9661 cgggctatgt ttttaccggg tggtcgggcg actgctcggg caccggctcc tgcagtgtct
     9721 ccatgacctc ttcacgtaac gtcaccgcga ccttcgtcgc gtcgtgtacc gtcaccaccg
     9781 attcgagttg gtccgcgtgt tcgacatcat gcggtccggg gacgcagtat aagtactatc
     9841 gtacctcacc gggcagcacc tgcgggggct actacaatgg acaggtcgcc gattcacgaa
     9901 gttgcaatct cggcgcgtgc ccggtccctc cgaccgcctc gctcaccgtg tcgccgaacg
     9961 tcgtccagta tggcggtacg acacaagtct catggtcctc cacgaacgcg gacacgtgct
    10021 atttctattg ggacggctcg ccatgggtaa cgaagggctc tggcggcagt ttcatgacga
    10081 acgccctcaa tatggccggg tatcattcca tcacgatgca gtgtaagaac accggcaacg
    10141 gccttacgag caactggcag acgggttccg tgaccgtcat ccctccatcg ccatccgggc
    10201 tcacgggctc ttgtaatggc cttaccgcac aggcgagttg gaacggtgtc gcgggagcga
    10261 gctcgtacgc cttccgctct gacaatacga ccataggcag ttggaacggt acctgcgatc
    10321 cgtcgaccgg agcgggagac atctgtgaga cgacgaccgc gacatcgcgt tcgtggtcgg
    10381 ccgggtacgg atacaactat tccgtgtggg tgcattcgtg tagcgcggtc ggctgttccg
    10441 cgacgccatc gtcgatctcg ttctcatgcc cggcggcgcc acagccggac ctcgtctccg
    10501 aagcaattct cgttcccgca tcggggacgg tcggcacgcc gctcgatctc gccgcaacgg
    10561 tacggaacgc cggcggcgcg ggaacgggcg tcgcgtttaa ggacgacttt agctaccgat
    10621 ggggcacgac cggcacgtgg acgtcgctcg gcctccttcc ggagagcgca cttgcctccg
    10681 gggcgagcgc gaacgacacc gatacctaca caccgacgca aacgggaacg ctttacatac
    10741 agcactgcgt cgattcgacg aacgtggtcg tcgaaggcgc gaacgaaacg ccgaactgtt
    10801 cggtcgcgcc ggcgatcacc gtatcagcaa gctcgagcgc gccgaccgtc gtcaccatct
    10861 cggacacgta cgcgccgggc gataccgccg tgacgctaaa cggcacggcg aatcccaatg
    10921 gctcggacac catcgcgcgc ttccgctggg gaacgacgga tcctctcggt gactgtgcgg
    10981 cgctcccgaa tcttaccgga aacgcgatgg tcggaaacgg gacgacgtcc gtgaactatg
    11041 cggggcttct cccgggcgtc acgactggca cgacgtacta ctactgcgcg tacggggcga
    11101 gtgccgccgg cgtaggctac ggggccgtcc gttccttcgg tgcgggaggc tccgccggta
    11161 tcgatctccg ctcggagaac ctctccgtgc catcctcggg caaggtcggt tcagcgctca
    11221 acttctcggc gatggtccac aacgtcggcg cgaccgccgc gggcgcgttc aaggacaact
    11281 ttagctacca atgggacggt acatcgggct cgtggaccga catcaatccc ttcacgaacc
    11341 acgcgagcct cgcggccggc gcgacctcga acgacaacgc ttcatacgta ccatcgcgaa
    11401 cgggtacgct ctacatccag cactgtgtcg attcggctaa cgacgtcgat gaaggcgcga
    11461 acgagacgcc gaactgtacg ataggggaca gcgacgcgag cgatggaggc ggcggaggtg
    11521 gcggtggcgg tggtggcgga ggcggtggag gaggcggaat tcccatcgtc gacggtgaaa
    11581 tagacggaga tccaaccatc gtcggttcga acggaacctc gaccatcacg tggtcgacga
    11641 acggcgcggg gaactgcacg gtctccggtg gtgcggatag ctggagcggc acctcgggct
    11701 ctcaagtgac gtcgccgctt aacgccgaca cgacgtacac gctcgtgtgc gacggcgtcc
    11761 tcatcgacag cgtcaccgtg caagtacgga cgacaccaga actcgtcgcg agcccacggg
    11821 tcgtgaacca gggaagccag acgacgctca cgtggcgtct caacggaggc gtgaactgta
    11881 cgctcacggg tggcggtctc aatctcggcg gtctcaacgg aaacagttcg gtgaacgtga
    11941 ccgtctccgc gaatacgacg tatacgctca catgtccggg tcgggggagc gcgtcgatca
    12001 cggtcgaggt catccccgtc gggttcgaaa cgtaggagta ataccataca tatgccagag
    12061 caacgaaata cagagaatga acgtcgcgac gagaagcgcg tgcagatact cgcggtcctc
    12121 gccgtcctca tcctcgtcac catcgcgctc gtcatgctcg tccgtgaacg gcaagagcgc
    12181 atgaacgccg cggacgttcc agtgtccact gcggcgaatg aggctgtatc ggtccccacc
    12241 gcatccgcaa acgcggcgtc catctacccg gcgaacgtgc gaaccgcctt cgacgagata
    12301 cggcgaaaag ttgcagaagg cgagatgaca ccgcaagatg cagacaccgc gacggcgaag
    12361 ctcatggacg acgtaccgac cccgtaaaac gccgtttcac atcatgacca gacacagcgc
    12421 taacgtactc gcgagcatag tcctcatcgg tgttctcatc gtcgcgatag gtacgacgct
    12481 ctggcggcgt acacacatga acgtgccatc cgtagacgtc gcgaaggacg aacctgccgc
    12541 cgaacccatc cgcgaacagt ggaccggtgt cgtccagtcc gtctcggccg actcgctcac
    12601 gctcaccgtc gtcgtgcctg aagggcaggg cgaccgaacc gtttcactta cgaacgatac
    12661 tattttcaaa aaagcgacgc tccgcgaccc ggatacggta aaacgagaga tggccgcctt
    12721 cgagaaggcc atggaagcgt accgcaaggc gtatgacgca tcgtcgaccg tatcaagtac
    12781 gcctccgccg gttctcccgc tcgccgcgac cgaggagacg attgctcccg ctgacattcc
    12841 cataggggcc tcggtgagcg tcgtcgcgac cgtccctggg tcggtaaacg gcgctgcggt
    12901 cgcggagagc gttgaggtta taccccccgc accgagcacg agcgagtagc gcccatttcc
    12961 gttgtgcaca agtcacggca tgctacaccc cgcttgctac acttaaggta agaacgcggc
    13021 tctgccgcgg attcgcatgc gtctcacaca tcagttcctc gatgcacgtt tcacgaggca
    13081 aggagccatt ctcctcgccg tggttgccgc gctcgttctc tcgtatacag tatctccgac
    13141 acgcgtcgag gcgggaagtg cgagcttcaa cctgaactgt gtatcgaatt gcagcacgga
    13201 ccccgacgtc ctcgcgacca tctcgacgaa caagaatacg tactctccgg gcgaggtagg
    13261 cagtctcacc atcaagtatg agagcgacgc cccagcgggc tcgagcttca caggaaacgc
    13321 gacttggacg ggtggtggtt cttggagcgg aagcgtgtcg cgactccaga tcacgacggg
    13381 cgcgacgata tcgatcggca gcttcacggt gccatcaacg ggaagctcgt atacacttac
    13441 cgtcaatgga agcttgagtc cgaactacaa cggggcagcc tcacagaaca tcgtcgtctc
    13501 gatatcacag ccgaaccaga cgctcacaac gagcgtgagc ggcggcggca ccatcacgtc
    13561 gagccccgcc ggcatcaact gtggtgcgac ctgctcggcg agctacgcct acggcacgaa
    13621 cgtctccctc accgcgacgc cctcatcggg gaataccttc accggctggt cgggtgcgtg
    13681 ttcgggaacc ggcggctgta ccgtctcgat gacgcaagcg aggaacgtga cggcctcatt
    13741 cggagccgcc tgcaccgtga cgcacgacac gagctggtcg gcctgtacgg catcctgcgg
    13801 tggcggttgg cagtacgagt actaccacac ctcgccggga agcacgtgcg gcgccatctc
    13861 gaacggacag gtgtatgcgc aacagcagtg taatacgcag gcgtgtcccg tcggtccgag
    13921 cgcgacgatg tgggtgagcc cttctccgat agcgtacggt ggttcaacgg tgtacggatg
    13981 gtcgacatcg aatgcgacgt actgccgttt ctatgtggat ggcaatctcg cgggtgatac
    14041 gaacaatggc tggccgatca acaatgccag tggctgggcc gacgcaccgc tctacaccga
    14101 taggacgtac cgtatcgaat gcacgaacgc gacgggacag tggacgtcgt cacaggcgac
    14161 cgtcgacatc accccgctca tcgtcctcga ttcggtgact tccggacagg tcgggtcttg
    14221 cgggttcgtt tccggttggg cattcgaccc gagccgccct tcgcagtcga tcaacgtcca
    14281 ccagtactac tacaatccgt tcaccggata cgacaccggg ccgacggcgg taaatcgcgc
    14341 ggatgttaat tcgacgtacg gcatcaccgg tgttcacgga ttccagtacc agataccgat
    14401 cgccgcggcg ggaacgtata ccgcgagaag ttacgcgata gggatcaacg ggagcggcgt
    14461 ccaaaacggg aacaatccgt tcgcgaccga gaagacgttc acgtgcgacc cggcgcccgt
    14521 caacaacgcc tcgtgtgtga gcgttaccgg cgttcccgcc tctatctacg cgggacagac
    14581 gttcacgccg accgtcacga cgtacaacaa cgggacgaag cagtggaatc gagatgggac
    14641 gccccacagc atcgggttcg cgacgccgta cgattcgctc ctcttcggcc tcaaccgcgt
    14701 ctccatcaac ccgaccacgg tgaaccccgg acagtactcg acgatgacgt tcaccgcgac
    14761 ggcgccgacc tccgcgggca cgtatcagtt cg
//
LOCUS       DASTSP010000011         9119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_922370, whole genome shotgun sequence.
ACCESSION   DASTSP010000011 DASTSP010000000
VERSION     DASTSP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922370"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFS75_00450"
     CDS             complement(<1..419)
                     /locus_tag="VFS75_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677172.1"
                     /translation="MRRAGSIIAVVLFIIGLYFMTTYVSPDALKRIAEANRLEGAILL
                     GFVMFGATVVAPIVSLPLVPMVAPFLGPFTTGVASYIGWSLGAFVAFFVGRRYGRPLV
                     GRFMNLESLARYESLIPPRTGFALIVMLRMVIPVDIL"
     gene            complement(439..774)
                     /locus_tag="VFS75_00455"
     CDS             complement(439..774)
                     /locus_tag="VFS75_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU4677173.1"
                     /translation="MRFLARLIITALSLVLVTYVVPGFEVSGFYPALIAALVLGILNA
                     IVRPVLIVLTLPITILTLGLFIFVINAALFLFAASFIQGFAIASFGAALLGSILVSAI
                     SWVANHFID"
     gene            complement(812..3334)
                     /gene="secA"
                     /locus_tag="VFS75_00460"
     CDS             complement(812..3334)
                     /gene="secA"
                     /locus_tag="VFS75_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU4677174.1"
                     /translation="MSFLDRFFGPTYEKELKAVRPIVDAINALEPTIAALDDQGLRAR
                     SMELKSRVAGGVSLDDVLPEAFALVREAAKRTLGQRHYDVQLIGGIILHRGKITEMRT
                     GEGKTLVGTLPAYLNALTGKGVHVVTVNDYLARRDATWMGQVYALLGLSVGVINQNGS
                     FLYDPNHVDEDALRDAEGSYKVVHDFLRPSSRKEAYDADITYGTNNEYGFDYLRDNIE
                     FDPRNIRQREHSYAIIDEVDSILIDEARTPLIISAPAQDSENLYEVFAKIAARLEPEV
                     DFVVDEKMKSATLTDHGVETAEKMLGIENIYTDRGIKYVHHLETAVRAKALYKRDKEY
                     VVRDDEVVIVDEFTGRLQPGRRWSDGLHQAIEAKEGVKIQRESRTYASITFQNYFRMY
                     EKLSGMTGTALTSQEEFYSVYRLDVIPVPTHRAVKRIDHNDLIFQSERGKVEAIIRRV
                     KEVHTKGQPLLIGTASIDKNEELSAALSAAGIEHAMLNAKNHEREGEIIAEAGKKGAV
                     TLATNMAGRGVDIKLGGPLASEAEATEVRELGGLFVLGTERHEARRIDNQLRGRSGRQ
                     GDPGETQFYVSMDDALMRVFASDTVKSMMGRFGIPEDQPIENGMITRALESAQKKIEG
                     FHFDSRKHVLAYDDVLNQQRQIVYAKRKRLLLGSIEDVKELAAEVVAYAPETETAMEA
                     RIKELGETQWYEILRRVMLQVSDILWVEHLEVMQHTRSSVSLRAYGQQDPLIEYRKEA
                     VRLFGEMQEAALSRVAELIPTIKVEAVDKEEEELKEAQKHIALQGGAENATEAEKGAA
                     PIRKEERHGRNDLVTVTNGTETETMKFKKAETRLKEGWRIVE"
     gene            complement(3750..4838)
                     /locus_tag="VFS75_00465"
     CDS             complement(3750..4838)
                     /locus_tag="VFS75_00465"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU4677175.1"
                     /translation="MPPFLKTQVPLAPYTTLGIGGPAEYFAHVDTEEKLLATLAWAHG
                     EGLPLTVLGGGSNVLVADEGIPGVVALVDLRGIEFREDGEAVLADVGAGETWDAFVET
                     AVLRGLFGLENLSGIPGKVGAAPVQNINAYGASVGDVIVSVRARRREDGKEMTFDHDA
                     CAFGYRDSFFKTKEGSAYVITRVTFRLMRNADATIAYRSSSQSIARKLAEAGITAPTP
                     VDVRAAVLDVRRNIGMLEGMYRSAGSFFKNTVVTKEVFERVRLIAKSQFAEKSARFAP
                     WFWELPGGMVKLSTAFLMECTPYNKTNFSEERFRDTVSLSPLHTLSIVNVGGATASDV
                     RAFAETIIAAVEETFGVRIEPEVRFLPE"
     gene            complement(4921..7101)
                     /locus_tag="VFS75_00470"
     CDS             complement(4921..7101)
                     /locus_tag="VFS75_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4677176.1"
                     /translation="MKDIAKWIVHAGIFAVPAVVLIISNSLFFPYITGKNFTFRIIVE
                     IIFAAWIILALYDVQYRPRFSWIIANFTVFLVVMFFADWFGKNRAASFWSNFERMEGY
                     VTLVHVFLYTLVVGSVLKTERLWNRFFMTTLGVAVVLSIYAFAQIAGDIKINQGGWRL
                     DGTLGNSAYMAIYMLFHTFIALFMLLRTKSRGMRYVFGALVVLFVYLLVQTATRGTVL
                     GLVGGSFIMVTYIALFAKGYPRVRKAAVGGLIAVVAVVGLFIAFKDSSFIQGNPYLQR
                     IASISLKEASNRFNIWSMAYEGVQEHPVLGWGQSNYNYVFNKYYRPELHGGEAWFDRV
                     HNIVMDWLIAGGFVGMIAYFSILGAAVYYLLYRPLRGDESFTVIERGLLLGLLAGYMI
                     HNFVVFDNIVSYIFYGTFLAFIHARVGRSIARVDALEVDSRVIEQIYAPVVGIAVAVV
                     VYYVNVPGILAAGDIISAFKEKDPNLVLVDFDRALSRHSFGDQEIREQMMQRVQPMLS
                     QQNLPDEFKQRAFKKVETELKNEVALKPDDARTRVFLASFYRLTNNVDEALAQLKVAR
                     ELSPKKQLIIFEQGMAEIQKKDYAAAEAFFKEAYDLGPQFDDSRTYYATAAIYNNDLA
                     TFEDLIKTPEQKRSFAINDLALQAAYQAKMYPLLIEMFETRIAANPSDPQLRTNLAYI
                     QNESGDEAGAIATLEEASKDIPTFKTQADSFIESIKSGSAKPAK"
     gene            complement(7172..8833)
                     /locus_tag="VFS75_00475"
     CDS             complement(7172..8833)
                     /locus_tag="VFS75_00475"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HEU4677177.1"
                     /translation="MHENADLLHLPRLNLKGRQIYDLEIMLVGGFEPVRGFMGEADYD
                     PVVETMRLSDGTLFPMPIVLDVPHESPYQVGDRILLCDPFGNPLATMDVTDRFTPDKE
                     KEALGVYKTLDKLHPGVQYLMDEMHDTYLGGPVTKIALPVRHDFKEFRKTPAELKALF
                     KERGWDKVVGFQTRNPMHRAHFELVRRAHEKTGAPVLVHPSVGMTKPGDIDYVTRVRT
                     YNVVCNTYGKDFTYLSLLPLAMRMGGPREALWHAIIRKNYGCTHFITGRDHAGPGKDS
                     TGKPFYGPYEARDLIVKHADEIGITAVPSEELAYSKTRGGYVAMNEVTDEDEIENISG
                     TEFRRRLRAGEEIPEWFSFKESIDILRQSVSRDSRPGVAIFFTGLSCSGKTTLAQHLL
                     ARLQILQDRNVTYLDGDVIREHLSKGLGFSKEDRDENVRRVGFVASEVVRHGGIVISA
                     LIAPYRDARRAVRSMVEHYGEFIEIFVDTSLEECARRDTKGLYEKAKQGLIKNFTGID
                     DPYEVPERPELVAKTEEATPEELVEEIIAYLYARGVLEKSEKSGS"
     gene            complement(8892..9050)
                     /locus_tag="VFS75_00480"
     CDS             complement(8892..9050)
                     /locus_tag="VFS75_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677178.1"
                     /translation="MRYNGGMYDDNYGCPCTDIQRFFVSERYPLKNLLPLVELQYRID
                     ELYKRLDE"
BASE COUNT         1766 a         2675 c         2809 g         1869 t
ORIGIN      
        1 cttaggatat cgacgggtat gaccatgcgg agcatcacga taagagcgaa gccggtccga
       61 ggcgggatga gcgattcata gcgcgcgagc gattcgaggt tcatgaaccg acctacgagc
      121 ggccgaccat agcgacgccc gacgaagaac gcgacaaagg cgcccaagga ccagccgata
      181 tacgaggcga cacctgtcgt gaaaggtccg aggaacgggg ccaccatggg tacgagcggc
      241 aatgagacga tcggtgcgac gaccgtcgca ccgaacatga cgaacccgag gaggatcgcc
      301 ccttcgagcc ggttcgcttc ggcgatcctc ttgagcgcat cgggagacac gtacgtcgtc
      361 atgaaataga gccctatgat gaagaggacg acggcgatga tactccctgc ccgccgcatg
      421 gaggacacag ttcccatact agtcgatgaa atggttcgcg acccaactga tcgcgctcac
      481 gagtatggag ccgaggagcg ccgcgccgaa gctcgctatc gcgaaaccct gtatgaacga
      541 tgccgcgaag agaaagagcg cggcattgat gacgaatatg aaaagaccga gcgtgaggat
      601 cgtaatgggg agcgtgagga cgatgagtac cgggcggacg atggcgttta gaataccgag
      661 gacgagcgcc gcgatgagcg cggggtagaa gcccgagacc tcgaaacccg gcacgacgta
      721 cgtgacgagg accaaagaaa gtgccgtgat gatgagccgc gcgaggaatc tcattcgtgc
      781 agtatacgct tcccttcctc gcggaacaac tctactcgac gatgcgccac ccttccttaa
      841 gccgcgtctc cgctttcttg aacttcatcg tctccgtctc tgttccgttc gtgacggtga
      901 cgaggtcgtt ccggccgtgc cgctcctcct ttcgtatggg tgccgcgccc ttttcggctt
      961 cggtcgcgtt ctctgcgccg ccctggagcg cgatgtgctt ttgcgcctct ttgagttcct
     1021 cctcttcctt gtccaccgcc tctaccttga tggtcgggat gagctccgcg acgcgcgaga
     1081 gcgccgcctc ctgcatctct ccgaagaggc gcaccgcttc cttgcggtat tcgatgaggg
     1141 gatcttgctg gccgtacgcg cgaagcgaca cgctcgagcg ggtatgctgc atgacctcga
     1201 ggtgttcgac ccaaaggatg tcggatacct ggagcatgac gcggcggagt atctcgtacc
     1261 actgcgtttc accgagctcc ttgatgcgcg cctccatggc ggtctccgtc tccggcgcgt
     1321 acgcgaccac ctccgccgcg agctccttca cgtcttcgat cgagccaagg agaagccgct
     1381 ttcgcttggc gtatacgatc tgtcgctgtt gattgaggac gtcgtcgtac gcgaggacgt
     1441 gctttcgaga gtcgaaatgg aacccttcga tcttcttctg cgcggactcg agggcgcgcg
     1501 ttatcatgcc gttctcgatg ggctggtcct cgggaatgcc gaagcggccc atcatcgact
     1561 ttaccgtgtc gctcgcgaaa acgcgcataa gggcatcgtc catcgagacg tagaattggg
     1621 tctctcccgg atctccctgg cgtcccgagc ggccgcggag ctgattgtcg atgcgccgcg
     1681 cctcatggcg ctcggtacca aggacgaaaa gtccaccgag ctcgcgaacc tcggtcgcct
     1741 cggcctcgct cgcgagcggc ccgccgagct tgatgtcgac gccacgaccc gccatattgg
     1801 tcgcgagcgt gaccgcgccc ttcttgcccg cctcagcgat gatctcgcct tcccgctcgt
     1861 gattcttcgc attgagcatc gcgtgctcga taccggccgc ggaaagggcc gccgagagct
     1921 cctcgttctt atcgatcgat gccgtaccga tgagaagcgg ctgacccttc gtgtggacct
     1981 cctttacgcg gcggatgatc gcctcgacct tgccgcgctc gctctggaag atgaggtcgt
     2041 tgtggtcgat acgcttcacc gctcggtgcg tcgggacggg gatgacgtcg agccggtaga
     2101 cggagtagaa ctcctcctgc gaagtgagcg cggtaccggt catacccgag agcttctcgt
     2161 acatgcggaa gtagttctgg aacgtgatgg aagcgtacgt gcgcgactcg cgctgtatct
     2221 tcacgccttc cttcgcctcg atcgcctgat ggaggccgtc ggaccagcgc ctgcccggct
     2281 ggaggcggcc cgtgaactca tcgacgatga cgacctcgtc gtcgcgaacg acgtactcct
     2341 tgtcgcgctt atagagtgcc ttcgcgcgga cggcggtctc gaggtgatgg acgtacttga
     2401 tgccgcggtc ggtgtagatg ttctcgatac cgagcatctt ctcggcggtc tcgacaccgt
     2461 ggtcggtgag ggtcgcgctc ttcatcttct cgtcgacgac gaaatcgact tccggctcga
     2521 gccgcgcggc gatcttcgcg aagacctcgt agaggttctc ggagtcctgc gcgggcgccg
     2581 agatgatgag cggtgttcgc gcctcgtcta tcaaaatgga gtcgacctcg tcgatgatgg
     2641 cgtacgagtg ctcgcgctgg cgtatgtttc ggggatcgaa ctcgatgttg tcgcgaagat
     2701 agtcgaaccc gtactcgtta ttagtgccgt acgtgatatc ggcatcgtac gcctctttgc
     2761 ggctcgaagg acgaaggaag tcgtggacga ccttgtacga accctcggcg tcgcggaggg
     2821 cgtcctcgtc gacgtggttc gggtcataga ggaaggaacc gttctggttg atgacgccga
     2881 ccgagagacc aaggagcgcg tagacctggc ccatccacgt cgcgtcacgt cgcgcgagat
     2941 aatcgttcac cgtgacgacg tggacgccct tcccggtgag cgcgttcaag tacgcgggga
     3001 gcgttccgac gagcgtctta ccctctcccg tccgcatctc ggtgatcttg ccgcgatgga
     3061 ggatgatgcc gccgatgagc tggacgtcgt aatgtcgctg accgagcgtt cgcttcgccg
     3121 cttcgcggac gagcgcgaac gcctccggaa gaacgtcgtc gagcgatacg ccaccggcga
     3181 cccgcgactt gagttccatc gaacgcgcgc ggagaccctg gtcgtcgagc gccgcgatcg
     3241 tcggttcgag cgcgttgatc gcgtcgacaa tgggacgaac ggccttgagt tccttctcgt
     3301 aggtcggtcc gaagaatcta tccaaaaatg acataatata gaggttttat agtcttccgg
     3361 ttcagaggga gagacgaatc cgtctagcat accacgtatt tcagccctaa aaaagcgaaa
     3421 acgctccctc gtagagggag cgttttcgag tgtgtcagaa gacaccttgg agcgaaagtt
     3481 actttcgctt tgcggtcttg cgctttgcgg tctttcgctt tgccgtcttc ttagcaggct
     3541 ttcgcttcgc tgccttcttc ttctttgctg gcatgtgtga agttgaaaat tttactcaat
     3601 aacaactcga ccgcattgtt ccctttccaa agagcatgtg caaagagaac atctgcttat
     3661 attcaattat cagttataaa tttttttgca caagaaaaaa tgaaaagaat gtgtggataa
     3721 actttttcaa aaaatgtatc tcgcaaaatt cactccggaa gaaaacgtac ttccggttcg
     3781 atgcgcacgc cgaacgtctc ctcgaccgcc gcgatgatcg tctcggcgaa cgcgcgcacg
     3841 tcggacgcgg tcgcaccgcc cacgttcacg atcgagagcg tatggagggg cgagagcgag
     3901 acggtatcgc ggaagcgctc ttcggaaaag ttcgttttat tgtacggtgt gcactccatg
     3961 agaaacgcgg tcgacagctt caccatgcca cccgggagtt cccagaacca cggcgcaaaa
     4021 cgtgccgatt tctcggcgaa ctgcgacttt gcgatgagtc gtacgcgctc gaacacctct
     4081 ttcgtgacga ccgtgttctt gaagaacgag cctgccgagc ggtacatccc ctcgagcatg
     4141 ccgatgttcc ttcgaacgtc gagaacggcc gcgcggacat cgacgggcgt cggcgcggtg
     4201 atgcctgcct cggcaagctt ccgcgcgatg ctctgcgacg aacttcggta cgcgatcgtc
     4261 gcgtccgcat ttcgcatgag acggaacgtc acgcgtgtga tgacgtacgc gcttccctcc
     4321 ttcgtcttga agaaactgtc gcgatagccg aacgcgcatg cgtcgtgatc gaacgtcatc
     4381 tctttgccgt cttccctacg tcgcgcgcga acggagacga taacgtcgcc gaccgacgcg
     4441 ccgtacgcgt ttatgttctg aaccggcgcc gcgccgacct tgccggggat gcccgagagg
     4501 ttctcgaggc cgaagagtcc ccggaggacc gctgtctcga cgaacgcgtc ccaggtctct
     4561 ccggccccga cgtccgcaag gacggcctcg ccgtcctcgc ggaattcgat accgcgcagg
     4621 tcgacgaggg cgacgacgcc gggaatacct tcgtcagcga cgaggacgtt cgagccaccc
     4681 ccgaggaccg tgagcgggag tccttcccca tgtgcccacg cgagggtcgc gaggagtttc
     4741 tcttcggtat cgacgtgcgc gaagtattcg gccggcccgc cgatgccgag ggtcgtatag
     4801 ggagcgagcg gtacctgcgt tttgagaaat ggcggcatac gggatggtgc gatttccgta
     4861 gtatagcagg tagcgcgcaa aaggcccccg gaggggcctt ttgcgcgccg tggctctcaa
     4921 ctacttcgca ggcttcgcgg aaccgctctt gatggactcg atgaaggaat ccgcttgggt
     4981 cttgaaggtc gggatgtcct tcgatgcctc ctcgagcgtc gctatcgccc cagcctcgtc
     5041 gcctgactcg ttctggatgt acgcgaggtt cgtacggagc tgcggatcgc tcgggttcgc
     5101 ggcgatacgg gtctcgaaca tctcgatgag aagcgggtac attttcgcct gatatgcggc
     5161 ctggagcgcg aggtcgttta tcgcgaacga gcgtttctgc tccggcgtct taatgaggtc
     5221 ctcgaaggtc gcgaggtcgt tgttgtatat cgccgcggtc gcgtagtacg tgcgcgaatc
     5281 gtcgaactgc ggcccgaggt cgtacgcctc cttgaagaac gcctcggccg ccgcgtagtc
     5341 cttcttctgt atctccgcca taccctgctc gaagatgatg agctgtttct tcggcgagag
     5401 ctcgcgcgct accttcagct gtgcgagggc ctcgtcgacg ttgttcgtga ggcggtagaa
     5461 cgaggcgagg aacactcgcg tacgggcgtc atcgggcttc agagcgacct cgttcttgag
     5521 ctccgtttcg accttcttga acgcgcgctg tttgaactcg tcgggaaggt tctgctggct
     5581 gagcatcggc tggacgcgct gcatcatctg ctctcgtatc tcctggtcgc cgaacgagtg
     5641 ccgcgacagc gcacggtcga agtcgacgag aacgaggttc gggtctttct ccttgaacgc
     5701 ggagatgatg tcccccgcgg cgaggatacc ggggacgttc acgtagtaca cgacgacggc
     5761 gacggcgatg ccgacgaccg gcgcatagat ctgctcgatg acacgcgagt cgacttcaag
     5821 ggcgtcgaca cgcgcgatac tcctccctac ccgtgcgtgg atgaacgcga ggaacgtccc
     5881 gtagaaaatg tagctcacga tgttatcgaa gacgacgaag ttgtggatca tgtaacccgc
     5941 gaggaggccg aggagaagcc cccgctcgat gaccgtgaac gattcgtcgc cccggagcgg
     6001 acggtagagg aggtagtaga ccgcggcacc gagtatcgag aagtacgcga tcatgccgac
     6061 gaagccgccc gcgatgagcc aatccatgac gatgttgtgg acgcggtcga accacgcctc
     6121 gccgccgtgg agttcgggtc ggtagtactt attgaacacg tagttatagt tgctctggcc
     6181 ccagccgaga accggatgct cctgcacgcc ctcgtatgcc atactccaga tattgaagcg
     6241 gttgctcgcc tccttgagcg atatcgaggc gatgcgctgt aggtacggat tcccctgaat
     6301 gaacgacgag tccttgaacg cgatgaagag gccgacgacg gcgacgaccg cgatgagccc
     6361 accgaccgcc gctttacgga cgcgcggata ccccttcgcg aagagcgcga tgtacgtcac
     6421 catgatgaag gaaccgccga cgagaccgag aacggtgccg cgggtggccg tctgcacgag
     6481 aaggtagacg aagaggacga cgagggcccc gaaaacgtat cgcattcccc tgctcttcgt
     6541 ccgaaggagc atgaagagcg cgatgaacgt atggaagagc atgtagatcg ccatgtacgc
     6601 cgagttgccg agcgtcccgt cgagacgcca cccgccctgg tttatcttga tgtcacccgc
     6661 gatctgcgcg aaggcgtaga tggacagaac gacggcgaca ccgagggtcg tcatgaaaaa
     6721 gcggttccag agccgttccg tcttgaggac gctcccgacg acgagcgtgt agaggaacac
     6781 gtggaccaac gtcacgtatc cttccatgcg ctcgaagttg ctccagaagc tcgcggcccg
     6841 attcttgccg aaccaatccg cgaagaacat gacgacgagg aagaccgtga agttcgcgat
     6901 gatccaagag aagcgcgggc ggtactgcac gtcatagagc gcgagaatga tccatgccgc
     6961 gaagatgatc tcgacgatta tcctgaaggt gaagttcttc ccggtgatgt acgggaagaa
     7021 gagcgagttc gagatgataa ggacgactgc gggaacggcg aagatgccgg cgtggacgat
     7081 ccactttgcg atgtctttca tattgccgaa aactatacca cgcaccgcct agaggtgtac
     7141 aaaaggcgaa gacgacggat ttcaggacgc gtcaagagcc gctcttctcg ctcttctcga
     7201 ggacgccgcg cgcatagagg tacgcgatga tctcctcgac gagttcctcc ggggtcgcct
     7261 cctcggtctt cgcgacgagc tcgggccgct cgggaacctc gtacgggtcg tcgatgccgg
     7321 tgaagttctt gatgaggccc tgcttcgcct tctcgtagag tcccttcgta tcgcgccgcg
     7381 cgcactcctc gagggaggtg tcgacgaata tctcgatgaa ctcgccgtag tgctcgacca
     7441 tggagcgaac cgcacggcgc gcgtcccgat acggcgcgat gagcgcggag atgacgatac
     7501 cgccgtggcg gacgacctcg ctcgcgacga agccgacgcg gcgcacgttc tcgtcgcggt
     7561 cctccttcga gaagccaagg cccttcgaga ggtgctcgcg aatgacgtcg ccgtctaagt
     7621 acgtgacgtt cctgtcctgc agtatctgga gtcgggcgag aaggtgctgt gcgagcgtgg
     7681 tcttccccga gcacgagagg ccggtgaaga atatggcgac gccggggcgg ctgtcgcggc
     7741 tcacgctctg cctaagaatg tcgatcgact ctttgaacga gaaccattcg ggtatctcct
     7801 ctcccgcgcg aagtcggcgg cggaactcgg tgcccgagat gttctcgatc tcgtcctcgt
     7861 cggtcacctc gttcatcgcg acgtacccgc cgcgcgtctt cgaatacgcg agctcctcgg
     7921 aggggaccgc cgtgatgccg atctcgtcgg cgtgcttcac gatgaggtct cgcgcctcat
     7981 aggggccata gaacggcttc ccggtgctgt ccttccccgg ccccgcgtgg tcgcggccgg
     8041 tgatgaaatg cgtacagccg tagttcttgc ggatgatggc gtgccagagc gcctcccgtg
     8101 ggccacccat gcgcatcgcg aggggtagga gcgagagata cgtgaagtcc ttgccgtacg
     8161 tgttgcagac gacgttgtac gtgcggacgc gcgtcacgta gtcgatatca cccggcttcg
     8221 tcatgccgac cgatgggtgg acgaggacgg gtgcgccggt cttctcgtgc gcccgccgga
     8281 cgagctcgaa gtgcgcgcgg tgcattgggt tccgcgtttg gaagccgacg accttgtccc
     8341 agcctcgttc cttgaagagc gccttcagct ccgccggggt ctttcgaaat tccttgaaat
     8401 cgtgcctaac cgggagcgcg atcttcgtca cggggccacc gaggtacgtg tcgtgcatct
     8461 cgtccatgag gtactggacg ccgggatgga gcttatcgag ggtcttgtag acgccgagcg
     8521 cttctttctc cttgtccggc gtgaagcggt cggtcacgtc catggtcgcg agcgggttcc
     8581 cgaagggatc gcagaggaga atgcggtcgc caacctggta cggactctca tggggcacat
     8641 cgaggacgat gggcatcggg aagagcgtcc cgtccgaaag gcgcatcgtc tcgacgaccg
     8701 ggtcgtagtc cgcctccccc atgaagccgc ggaccggctc gaagccgccg acgagcatta
     8761 tctcgaggtc gtatatctgg cgccccttca ggttgagtcg cggtaaatgg agcaggtcgg
     8821 cgttttcgtg catgtacgtt gattagttag gtaacgcaca ctataacacc agtccactct
     8881 gaaaagtaat actactcgtc gagccgctta tagagttcat cgatacggta ctgcaattcc
     8941 acgagcggaa gaaggttctt tagcgggtat cgctcgctca caaagaatcg ttggatgtcg
     9001 gtgcaggggc atccatagtt atcatcgtac atacccccgt tatagcgcat acttccctga
     9061 tagtgtgcta aaaattcaac ataggaatca gttcctgtcg cgtagcgcat gaacacgtc
//
LOCUS       DASTSP010000012        15019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1147239, whole genome shotgun sequence.
ACCESSION   DASTSP010000012 DASTSP010000000
VERSION     DASTSP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147239"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFS75_00485"
     CDS             <1..880
                     /locus_tag="VFS75_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU4677179.1"
                     /translation="TEYELIAGERRLRASKLAGLTQVPVIIRSGEESELMKLELAIIE
                     NLQREDLNAVDRAYAFKQLAEQFGLSHAQVAEKVGRSREYVSNSIRLLALPETILDAL
                     RRNDISDGHARTLLMLNDRPEEQDVVFREILLKKLSVREVERISRKIATDKVRKKDWQ
                     NTDPELIEIEKQFTETFGTRVQIQKTDFGGKLTIDYFSAEDLRKVLDMIHKGENATGE
                     TEGNILMKVSEATPLEPAPTESPVAGVVTAAPEAAPDAEEEVFTRHDTLEVPETPAEE
                     LDSDEGDLYSLKNFSL"
     gene            complement(861..2432)
                     /locus_tag="VFS75_00490"
     CDS             complement(861..2432)
                     /locus_tag="VFS75_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4677180.1"
                     /translation="MVNTTADTIIDNLASPSDTDRELVRRAYAFAERAHEGRTRYSGE
                     PYMTHLAEVGRMLAEIGMGASTVAAGLLHDTIEDTNVTPDELGAEFGEEILFLVEGVT
                     KLGSVRYYGSDRHNESLRKLFVATSQDIRVLIIKLVDRLHNMQTLEFVPKEKQLRIAR
                     ETLEIYVPVTHRLGMGRIRKELEDLAFPYVYPEEHKRVVDLTKHIMKKSEADLEKARK
                     ALQKRLAESGIRNFHTTFRAKGTYSLYQKLKRRDWDMDKVYDYLAVRVIVETIEQCYQ
                     TLGIVHELWRPIPKRVKDYIAFPKPNGYQSLHTTVITPNRTILEVQIRTEAMHREAEY
                     GIASHIVYKTKMQGQKPPTGARSWFSSLVPSLFRPFIWRTNVKSEREKTLIQERNHKK
                     KIPQWIQEIANAHKTHDGSREFFEGLQDDFFSYRIFVFTPEGDVVDLPVGASPIDFAY
                     AIHSDIGDHVSGAKVNNKLVGLDTELHNGDIVEIITGKSNKPTRKWLDYAKTSLARRR
                     IRTALSLQTPTEKSS"
     gene            complement(2499..4862)
                     /gene="topA"
                     /locus_tag="VFS75_00495"
     CDS             complement(2499..4862)
                     /gene="topA"
                     /locus_tag="VFS75_00495"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU4677181.1"
                     /translation="MSKTLVIVESPAKAKTIEKYLGPGYVVRASVGHVRDLPKSNKDA
                     VDIEGGFVPKYITSVGKDKVIADLKKAAKDADEILLATDPDREGEAIAWHVSELIKDV
                     AAKKPRRIVFYEITEPAVKEALEHPRAIDEHLKEAQEARRVLDRLVGYDLSGLIWKKV
                     RYGLSAGRVQSPALRIVMEREREIRAFVPEKYFVLTADTKTEKGTNLPLTGSVEPKEE
                     EEAKRIVKVGEAHSWKITAVTKSEQKRSPRPPFTTSTLQQVASTRLGFSPSRTMGAAQ
                     KLYEAGYITYMRTDSTNMSAVAQKQILALVADEFGKEYVAPRTYKTKSKSAQEAHEAI
                     RPSNFAKRSLGHTEDQKRLYELIWERAVSSQMSDATIERTKIVANVVGAKEEIPNFTA
                     NGSRVLFPGWLTVDTRARGEDIEIPKLASGDPLTLVSISYEGKETQPPSRYTEAGLIK
                     ELEKRGIGRPSTYASIMKTIVDRGYVVKEGRTLMPTDTGDLVSSFLEEHFTEYIGDNF
                     TSEMEDELDEIAEGKRGYTETLSSFYGPFRKAVAGKENIAKITNLGPGPKEFPCPTCG
                     SDMVVKLGRGGKFLSCGRYPDCEGARLIDGSEIKEDEPLGKHPETGEDIYLLTGRFGP
                     YVQLGKTPEKVRGKKTPTPKRSGVPKGKPVSEVTLEDAVQYLRLPRELGAHPETGEPI
                     VSNVGRFGPYIAHAGDFRSLKGTDNPYDITFDRALTILKEPKKMRAGEKLVKEVGLNP
                     KTKKMIRVFESKSGRYLKRGFARVWLPEGTDLDAFTVEDAVELLGKK"
     gene            complement(4969..5133)
                     /locus_tag="VFS75_00500"
     CDS             complement(4969..5133)
                     /locus_tag="VFS75_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677182.1"
                     /translation="MPDDPRRRWEQKRWIRDCVIYYLEEDGIYRDGDGNQLPKREDVV
                     DFPEIIEFFH"
     gene            complement(5192..6046)
                     /gene="dprA"
                     /locus_tag="VFS75_00505"
     CDS             complement(5192..6046)
                     /gene="dprA"
                     /locus_tag="VFS75_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU4677183.1"
                     /translation="MEFPITKVVCSDLSPLLVEIPDAPTHLYVRGELSPTNKRLAVVG
                     SRNYSNYGKQAVERLIEGLRGYPVSVVSGLALGIDALAHEAALRAHLHTIAVPGSGIA
                     DRVIYPRRNRGLAARILESGGALVSEFEPDFEATPWSFPKRNRIMAGLSHAVLLIEAS
                     ERSGTLITARLAADFNREVLVVPGSIFSENSHGVHQFLKLGATPVTSPDDIIDALGFA
                     EHVSARTTPPVSDDESAVLAVLSEPRDHDSIIRLLPFPRERTLVLLMEMELAGLVRDE
                     NGIVYRAD"
     gene            6154..6504
                     /locus_tag="VFS75_00510"
     CDS             6154..6504
                     /locus_tag="VFS75_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677184.1"
                     /translation="MNKLRHQGFRYLEVIPDPAGQPTTYVTVTELMDKAAKEGYYPLP
                     VGDAEEIIQELQKRMTPDIHRYFLVQRVEGEKRDDIGMVYSIQRDYVCPMYADLTLIT
                     GCDGRQRYVFAQPA"
     gene            6599..7861
                     /gene="serS"
                     /locus_tag="VFS75_00515"
     CDS             6599..7861
                     /gene="serS"
                     /locus_tag="VFS75_00515"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU4677185.1"
                     /translation="MLDIKFIRENLDLVKQGAAKKRVAIDLDALIATDDKRKALLSSI
                     EAKKAEQNAASRDVAGAADESTRSTLIAKMKVLKDEMQKEEDELKSVMQKWQELMLQV
                     PNIPDMSVPDGESDAQNAEVSAWGEKTAFDFEPKSHVDIMLKLKMVDFERGTKVHGFR
                     GYYLINQGARLSWAIWNYAQQFFLAKGMSPMLVPAIVRKSNLYGTGHLPNDVEDIYRT
                     QDDDYLIGTAEVSTMGYYTDEVLDIAELPIKWLSFSPCFRREAGSHGKDTKGLIRVHE
                     FFKFEQVILCEASHEESVKWHEWLTRNTEEFIESLGIPYHRVVNCGGDLGLGQVKKYD
                     IELWVPGENRYREIASASYFHDFQTRRFNIRYKDKDGKMRYAHSLNNTAIPTPRILVS
                     LIENFQQADGTVKLPPVLAPYFGADVLQ"
     gene            7868..8716
                     /locus_tag="VFS75_00520"
     CDS             7868..8716
                     /locus_tag="VFS75_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677186.1"
                     /translation="MQRERRTFGKRATARRGSNFARQLVFGGLLLVTLGLLGSGVWYA
                     TRLPALTIAAVEVDGGETISHDTVRTRVENVLSGSYVLLVPKRFSYLYPHDAIVASLS
                     AVPRVHDVSVERTSRNELAVSFDEYVPHALWCASGASSTPCWFIDDTGYAFSAAPDLR
                     GGTLVRHLDENATDVHEGQVIDAAKLAAVDRFISRAERELHFRITALTYRKDGDVEFS
                     VNGGGMILASADKDLDAAFDNLKTVLESPKFSHLAPGTFKYVDVRLESKIFVNETTDT
                     DEKLPE"
     gene            8742..9758
                     /locus_tag="VFS75_00525"
     CDS             8742..9758
                     /locus_tag="VFS75_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEU4677187.1"
                     /translation="MSFWDSSPRPLMVLAPLANVTDAAFRRIIAKYSAHRRKDGTVGG
                     PDVMWTEFVSADGLVRATPEGRAKLMADLIYTEAERPIVAQLFSSNPEHMERSARLCR
                     ELGFDGVDINMGCPDRTVEKQGAGAAMIKHPEAAREVIRAAKRGAGELPVSVKTRVGY
                     NKIELETWIPALLEERPAALTLHARTRKEMSKVPARWEHVREAVAIRDAMQGGSERTL
                     ILGNGDASSLADAEAKVRETGADGVMFGRAIFGNPWFFDPSVDVDALPLSERMRVMLE
                     HTRLFEELLPHKNFALMKKHYKAYVNNFDGAKELRATLMELDSSDAIEAAVSAWLAER
                     GLSL"
     gene            9830..10138
                     /locus_tag="VFS75_00530"
     CDS             9830..10138
                     /locus_tag="VFS75_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677188.1"
                     /translation="MNDVLYTNLFFSITALAVVVFTVFLCIAAYHVIKALRVLRRILD
                     RIDAGADVIAGDVRSVRAFFAEGGFLRRLVSLLTGREGRSREHRTRERRKQDLKTSDE
                     "
     gene            10146..10565
                     /locus_tag="VFS75_00535"
     CDS             10146..10565
                     /locus_tag="VFS75_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677189.1"
                     /translation="MAKKTKESMSTAQKVGLGLGLTAAAVTAAGAYFLYGTKESAQNR
                     KRVKGWVLKAKGDVLEALEKAKTITEKEYNDLVDAATKAYGTVSKASQGEVRDFKKEM
                     QGHWLELQKSKAVKKLMSDVKPAKKTARRKTPAKKTK"
     gene            10618..11451
                     /locus_tag="VFS75_00540"
     CDS             10618..11451
                     /locus_tag="VFS75_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4677190.1"
                     /translation="MNRRIILYSLFLMAIFGLAALVAFVGPHLGIMRGGLTAEVSAPA
                     GLTTDTWGIFDPATGELIAGEHASDARPIASVAKLFTAYAVMASEKRDDLVTISAGDV
                     ATEGRAGKLYVGERMTPYKLLFPMLIESSNDAAEAARRTLGVDFTKNVTNIESDLGLK
                     NTTLADGAGLSPKSVSSVEDLSRFYAHLKKTYPHVLDITRLNMYIDDHTGYVNNDPAR
                     SLTTFEGGKNGYTDEAARTFVGTFSKDGAEVGIVLLGSTDIVRDIERLLAFRKSPKGG
                     F"
     gene            11464..12495
                     /gene="dnaX"
                     /locus_tag="VFS75_00545"
     CDS             11464..12495
                     /gene="dnaX"
                     /locus_tag="VFS75_00545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU4677191.1"
                     /translation="MSGIQLALYRKYRPTSFDEVRNQEHVISVLKGAIEKKQVPHALL
                     FTGPRGTGKTTVARIFAKAVGASDVDTYEIDAASNRSIDDVRELKEAVSTLPYESPYK
                     VYIVDEVHMLTKEAFNALLKTLEEPPAHVIFILATTERDKLLDTITSRCQVFEFHAPT
                     REELRETVIAVAKREKFKLAPHVADVIALSADGSYRDALGITQKVMLASGDRELGEDE
                     VARIVGAPRSAVLSEVVAALASKDADRGLRALSEAETAHVDMKFFLKLLLERLRAVVL
                     LRNTKDKDALLSRFGDEEGKTLLSYANDAKSPINSHLLLRAIELGGYVGKSFAPTLPI
                     ELLLIEQHA"
     gene            12498..13139
                     /locus_tag="VFS75_00550"
     CDS             12498..13139
                     /locus_tag="VFS75_00550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4677192.1"
                     /translation="MHVYFVRHGETTMNRRLTHQPPGTPLSERGREQAREVAEFLRSV
                     NPDLLLTSDYTRALETARIIGLSVGLTPRIPGFLHELVRPSRLFHRSHFAPCTIWYSF
                     LTALHRNDLSWHFEDAENFPELAGRTRRALQYLESLSATHRSVVVVSHTVFINVLVAY
                     LCRERMLDVVDLFRVFFHFERLKNGGVVHVEFSGRRERGTCPWQLVPMAPDAL"
     gene            13149..14171
                     /gene="mltG"
                     /locus_tag="VFS75_00555"
     CDS             13149..14171
                     /gene="mltG"
                     /locus_tag="VFS75_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU4677193.1"
                     /translation="MERRHAIRRGKRTMTVVAVSLLSFAFVLWGVATFMLGGTNVRYE
                     GVNASLTNASAFPVGVDPREKRIVEVPNLDAYIEREGFAATRASSRQLTWVSRVMGQL
                     ALMDWFQNLASLSSRTLVIEPGERGEEVADHFAKILGWSEDERAAFVRAVAQRTPAIR
                     EGKFYPGRYTVPRDASPEAVADAVLGSFTENVLSRYATSTAAAVPLQDALTIASLLER
                     EAYDFDDMRLIAGVIWNRLFIGMNLQIDATLQYAKGTKPSEPWWPQVGPADKYIESAY
                     NTYENPGLPPAPIANPSAEAILAALNPKNTDCLYYFHDKKAGFHCAATYDEHVDLLKR
                     YYGRGK"
     gene            14192..14821
                     /locus_tag="VFS75_00560"
     CDS             14192..14821
                     /locus_tag="VFS75_00560"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU4677194.1"
                     /translation="MLGALESMVESLAAVVPLEVFVTVASILEEVLAPIPSPTVMMLT
                     GALASVQDRTLLMLVPLLLLGTIGKTLGASVVYVIADRGEDLIIGRFGRYFGVTREDV
                     EAFGDELGTGARGYFLLTLFRALPVVPSTLVSVGSGLIKVPYRMFLIATLVGTIVRDG
                     FYLYAGYIGTETFAAMVNHSASAETYIEAAALVVVFVYIAYRLMRQKRR"
BASE COUNT         2886 a         4618 c         4609 g         2906 t
ORIGIN      
        1 gaccgagtac gagctcatcg ccggcgaacg gcgacttcgc gcctcgaagc tcgccggact
       61 cacgcaggtc cccgtcatca tccgctcggg tgaggaatcc gagctcatga agctcgagct
      121 cgccatcatc gagaatctcc agcgcgagga cctcaacgcg gtcgaccgcg cgtacgcgtt
      181 caagcaactc gcggagcagt tcgggctctc tcatgcgcag gtagccgaga aggtgggaag
      241 gagcagggag tacgtgtcga actcgatacg cctcctcgcg ctccccgaga cgatcctcga
      301 cgcgcttcgg cgaaacgaca tctccgacgg gcacgctcgc acgcttttga tgcttaacga
      361 ccgtcccgag gagcaggacg tcgtcttccg tgagatactc ctcaaaaaac tttcggtccg
      421 cgaggtcgaa cgcatctcgc gaaagatcgc gaccgataaa gtacgaaaga aggactggca
      481 gaacaccgat cccgagctca tcgagataga gaagcagttc accgagacgt tcggcacgcg
      541 cgtgcagata cagaagaccg atttcggcgg gaaactcacc atcgactact tctcggcgga
      601 ggaccttcgg aaggtgctcg atatgataca taagggcgag aatgcgacgg gagagacgga
      661 gggtaatatt ctcatgaagg tctcggaggc gacgccgctt gagcctgctc cgacagagtc
      721 tccggtcgca ggcgtggtga cagcggctcc cgaagcggca ccggacgccg aggaagaggt
      781 cttcacgcgc catgacacgc tcgaggtccc ggagacgccc gccgaggagc tcgattccga
      841 cgagggtgac ctctactcgc tcaagaactt ttctctgtag gcgtctggag cgagagcgcc
      901 gtacgaatgc gacgtcgcgc gagcgacgtt ttcgcgtagt cgagccactt gcgcgtgggc
      961 ttattggatt tgccggtgat gatctcgacg atgtcgccgt tgtgaagctc ggtatcgagg
     1021 ccgacgagct tgttgttcac cttcgcgccg gagacgtggt cgccgatgtc cgaatggatc
     1081 gcgtacgcga agtcgatggg cgaggcgccg acagggagat cgacgacgtc gccctccggg
     1141 gtgaagacga agatgcggta ggagaagaaa tcgtcctgga gaccctcgaa gaactcgcgc
     1201 gaaccgtcgt gcgtcttatg cgcgttcgcg atctcctgta tccactgggg gatcttcttc
     1261 ttgtggtttc gctcttggat gagggttttc tcccgctcgc tcttcacgtt cgtgcgccag
     1321 atgaacggac gaaagaggct cggtacgagc gaactgaacc acgagcgcgc gccggtcggc
     1381 ggcttttgtc cctgcatctt cgtcttgtag acgatgtggg acgcgatgcc gtactcggct
     1441 tcgcggtgca tggcctcggt ccgtatctgg acttcgagaa tggtccggtt cggcgtgatg
     1501 accgtcgtat ggagcgactg atacccgttc ggcttcggga aggcgatgta atccttcacg
     1561 cgcttcggaa tggggcgcca gagctcgtgg acgatgccga gggtctggta gcactgctcg
     1621 atcgtctcga cgatgacgcg gaccgcgagg tagtcgtaca ccttatccat gtcccaatcg
     1681 cggcgcttca gtttttggta gaggctgtac gtacccttcg cgcggaacgt cgtatggaaa
     1741 ttgcgaatgc cgctctcggc gaggcgcttc tgaagcgctt tgcgcgcctt ctcgaggtcg
     1801 gcctcgctct tcttcatgat gtgcttcgtg agatcgacga cccgcttgtg ttcctccggg
     1861 tagacgtacg ggaaggcgag gtcctcgagc tcctttctga tgcgccccat accgagtcgg
     1921 tgcgtgaccg gcacgtatat ctcgagcgtc tctcgtgcga tgcggagttg tttctccttg
     1981 gggacgaatt ccaaggtttg catgttgtgg agtcggtcga cgagtttgat gatgagcacg
     2041 cgaatgtcct gcgaggtcgc gacgaagagc tttcgaagcg actcgttgtg ccggtcgctt
     2101 ccgtagtagc gtaccgagcc gagcttcgtg acgccttcga cgaggaagag tatctcttcg
     2161 ccgaattcgg cgccaagctc atcgggcgtg acgttcgtgt cctcgatcgt gtcatggagg
     2221 agacccgcgg cgaccgtgct cgcgcccatg ccgatctcgg cgagcatacg tccgacctcg
     2281 gcgaggtgcg tcatgtacgg ctcgccggag tatcgcgttc gtccctcgtg cgcgcgctcg
     2341 gcgaaggcgt acgcgcgtcg aacgagctct cggtcggtat ccgaaggcga ggcaagatta
     2401 tcgatgatgg tgtctgcggt cgtattcacc atgttctcta tagtacacct gcggtgcgtg
     2461 acgacaaaaa gacgcgcgaa cgcgcgtctc cataatactt acttctttcc gaggagctcg
     2521 accgcgtcct cgacggtgaa cgcatcgagg tcggtccctt cggggagcca gacccgcgcg
     2581 aagccgcgct tcaagtatcg gcccgacttc gattcgaaga cgcgtatcat cttcttcgtc
     2641 ttggggttga ggccgacctc cttcacgagc ttctcgcccg cgcgcatctt cttcggctct
     2701 ttgagtatgg taagcgcgcg gtcgaacgtg atgtcgtacg ggttatcggt acccttcagc
     2761 gaccggaagt cgcccgcgtg cgcgatgtac gggccgaagc ggccgacgtt cgagacgatg
     2821 ggttcgccgg tctccgggtg cgcgccgagc tcgcgaggga gtcgtaaata ctgaaccgcg
     2881 tcttcgaggg tcacttcgga gacgggcttg cccttcggta cgcctgaacg tttcggcgtc
     2941 ggcgtcttct tgccacggac cttctcgggg gtcttgccga gctggacgta cgggccgaag
     3001 cgacctgtga ggagatagat gtcttcgccg gtctccgggt gctttcccaa cggttcgtcc
     3061 tcctttatct cggagccgtc gatgaggcgg gcgccctcac agtcggggta gcgaccgcac
     3121 gagaggaact tgccgccgcg accgagcttc acgaccatgt cgctcccgca cgtcgggcaa
     3181 gggaactcct tcgggcccgg tccgaggttc gttatcttcg cgatgttctc cttccccgcg
     3241 accgccttcc tgaaagggcc gtagaaggac gagagggtct ccgtgtaccc gcgcttgccc
     3301 tccgcgatct catcgagctc gtcctccatc tcgctcgtga agttgtcgcc gatgtactcg
     3361 gtgaaatgct cttcgaggaa cgatgagacg aggtctcccg tatcggtcgg catgagggta
     3421 cgtccttcct tcacgacgta gccccggtcg acgatcgtct tcatgatgga cgcgtacgtc
     3481 gacggtcggc cgatgccgcg tttctcgagc tccttgatga gccccgcctc ggtgtaccgc
     3541 gacggcggct gggtttcctt gccctcgtag gagatcgaga cgagggtgag cgggtcaccc
     3601 gaggcgagct tcggaatctc gatatcctcg ccgcgcgctc gcgtgtcgac cgtgagccag
     3661 ccggggaaga gaacgcgcga gccgttcgcg gtgaagttag ggatctcctc tttcgcgccc
     3721 acgacgttcg cgacgatctt cgtgcgctcg atagtggcgt cgctcatctg cgaggaaact
     3781 gcgcgttccc aaatgagctc gtagaggcgc ttctggtcct cggtatggcc gagggagcgc
     3841 ttcgcgaagt tcgagggtcg gatggcctca tgtgcctctt gcgccgactt actcttcgtc
     3901 ttgtacgtcc gcggcgcgac gtactccttg ccgaactcgt cggcgacgag cgcgagtatc
     3961 tgcttctgcg ccaccgcgct catgttcgtc gagtcggtgc gcatatacgt aatgtagccg
     4021 gcctcgtaga gcttttgcgc ggcacccatg gtcctcgacg gcgagaaccc gagacgcgtc
     4081 gaggcgacct gctggagggt cgacgtggta aagggcggcc gcggactccg cttctgctcc
     4141 gacttcgtga ccgcggtgat cttccacgaa tgcgcctcgc cgaccttgac gatgcgcttc
     4201 gcctcttcct cctctttagg ctcaacgctt cccgtgagcg ggagattcgt tcctttctcc
     4261 gtcttcgtgt ccgcggtcaa tacgaaatac ttctccggaa cgaacgcgcg tatctcgcgc
     4321 tcgcgctcca tgacgatgcg gagcgcgggc gattgcacgc gtcccgccga gagaccgtac
     4381 cggaccttct tccagatgag gcccgagaga tcgtacccga cgaggcgatc gaggacgcga
     4441 cgcgcctcct gcgcctcttt gaggtgctcg tcgatcgcgc gcgggtgttc gagcgcttcc
     4501 tttaccgccg gctcggtgat ctcgtagaag acgatacgtc gcggcttttt cgccgcgacg
     4561 tccttgatga gttccgagac gtgccatgcg atggcctcgc cctctcggtc ggggtccgtc
     4621 gcgaggagaa tttcgtcggc gtccttcgcc gccttcttca ggtccgcgat caccttatcc
     4681 ttgccgaccg aggtgatgta cttcggcacg aagccgccct cgatgtcgac ggcgtctttg
     4741 ttcgatttcg gaaggtcacg cacgtgcccg accgacgcgc gcacgacgta ccccggaccg
     4801 agatacttct cgatcgtctt agccttcgcg ggcgactcta cgatgacgag cgttttagac
     4861 atgtctccct ttcatactag ggaaaatacc ccatgtcaaa tgagaacccc gtgaaggtca
     4921 aagaaaatgc ccgcgcgatg cgcgggccgg aactagtcct ctcgtgtgtc agtggaagaa
     4981 ctcgattatc tcgggaaagt cgaccacatc ctcgcgtttc gggagttgat tcccgtctcc
     5041 gtcgcggtag atgccgtcct cctcgaggta gtagatgacg caatcgcgta tccaccgctt
     5101 ttgttcccac cgtcgtctcg ggtcatcggg catagctcgt cctccttgtc agccgtcgcg
     5161 atgatacgcc atggggtgcg gacgcgcaac gttagtcggc gcgatacacg atgccgttct
     5221 cgtcgcgaac gagtcccgca agttccatct ccataagaag gacgagggtc cgctctcgtg
     5281 ggaagggaag gaggcggatg atggaatcgt ggtcccgcgg ctcgctaagg acggcgagga
     5341 ccgccgactc gtcgtccgag actgggggtg tcgttcgtgc ggagacatgt tcggcgaatc
     5401 cgagcgcgtc gatgatgtca tcgggtgacg tgaccggtgt cgcaccgagc ttgagaaatt
     5461 ggtggacccc gtggctgttc tccgagaaaa tgctcccggg tacgacgagg acctcacgat
     5521 tgaagtcggc ggcgagtcgc gcggtgatga gcgtgcccga acgctcgctc gcctcgatga
     5581 ggaggacggc atgcgagagt cccgccatga tgcggttccg tttcgggaac gaccacggcg
     5641 tcgcctcgaa gtccggctcg aattcggaga ccaaggcacc gcccgattcg agaatacgcg
     5701 ccgcgagtcc gcggtttcgc ctcggataga tgacccggtc ggcgataccc gagccgggga
     5761 cggcgatcgt gtggaggtga gcgcgcaagg ccgcctcgtg cgcgagggcg tcgatgccaa
     5821 gggcaagacc ggagacgacg gaaacggggt agccgcggag tccctcgatg agacgctcga
     5881 ccgcctgttt cccatagttc gagtagtttc gagatccgac gacggcaagc cgcttattcg
     5941 tgggagagag ctccccgcgc acatataaat gtgttggcgc atcggggatc tcgacaagga
     6001 ggggagatag atcgctacag accactttcg tgatcggaaa ttccatacgt aaagtatacc
     6061 aggtgcggca agagatgatt gacaaaaacc attttgcata gtagaactca tagcggtatt
     6121 ttcggttgtt ccccacacat aggaggagca tgcgtgaata agttacgcca tcaaggcttc
     6181 agatacctgg aagtcatccc ggaccctgct ggtcaaccca ccacgtacgt cacggtgacg
     6241 gagctcatgg ataaagccgc aaaggaaggc tactatccgc ttcctgtggg cgatgcggaa
     6301 gagatcatcc aggagctcca gaagcgcatg acgcccgaca tccaccgcta cttcctcgtc
     6361 cagcgcgtcg aaggagagaa acgagacgac atcggcatgg tgtactcgat ccaacgggac
     6421 tacgtctgcc ccatgtatgc cgaccttacg ctgatcaccg gctgcgacgg taggcaacgc
     6481 tacgtcttcg cgcagcctgc gtgaaaaacc ggcgcacgga agccgggacc tcgcgtcccg
     6541 gctttttctt ttgagtaaaa agaaaacgtg cgtataatgg aattctatct agattcccat
     6601 gctcgacatc aaattcattc gggagaacct cgacctcgtg aagcaggggg cggcgaagaa
     6661 gcgcgtcgcc atcgacctcg atgcgctcat cgcgaccgac gacaagcgga aggcccttct
     6721 ttcctccatc gaggcgaaga aggcggaaca gaacgccgcg tcccgggacg tcgcgggggc
     6781 agcggacgaa tcgacgcgga gcacgctcat cgcgaagatg aaggttctca aggacgagat
     6841 gcagaaggag gaagacgaac tgaagagcgt catgcagaag tggcaggagc tcatgctcca
     6901 ggtgccgaac atccccgaca tgtccgtccc cgacggtgag agcgatgcgc agaacgccga
     6961 ggtgagcgca tggggcgaga agaccgcgtt cgacttcgaa ccgaagagcc atgtcgacat
     7021 catgctgaaa ctgaagatgg tcgatttcga acgcggcacg aaggtccacg gcttccgcgg
     7081 ctactatctc atcaatcaag gcgcgcgact ctcatgggcg atctggaact acgcacagca
     7141 gttcttcctc gcgaagggga tgagccccat gctcgtgcct gcgatcgtcc gaaagtcgaa
     7201 cctctacggt accgggcatc tccctaatga cgtcgaggac atctacagga cgcaggacga
     7261 cgactacctc atcggcaccg cggaggtctc gacgatgggc tactacactg acgaggtcct
     7321 cgatatcgcc gagctcccca tcaagtggct ctcgttctcg ccgtgcttta gacgggaagc
     7381 ggggagccac gggaaggata cgaaggggct catccgggtc catgagttct tcaagttcga
     7441 gcaggttatc ctttgcgagg cgagccacga ggaatccgtg aagtggcacg aatggctcac
     7501 ccggaacacg gaggagttca tcgagtcctt gggcattccg taccaccgcg tggtgaactg
     7561 cgggggcgac cttggcctcg gacaggtgaa gaagtacgac atcgagctct gggtccccgg
     7621 cgagaaccgt taccgcgaga tcgcgtccgc atcctacttc catgacttcc agacccgtcg
     7681 tttcaatatc cggtacaagg ataaggatgg gaagatgcgc tacgcgcact cccttaacaa
     7741 caccgccatc ccgacgccgc gcattctcgt ctctctcatc gagaacttcc agcaggcgga
     7801 cggcaccgtg aagctacctc cggttctcgc tccgtatttc ggggctgatg tactacaata
     7861 gtcgataatg cagcgagaac gccgaacctt cggaaagcgc gcgaccgcac ggcggggttc
     7921 taacttcgcg cgacaactcg tcttcggcgg cctgcttctc gttacgctcg ggctcctcgg
     7981 ttccggcgtg tggtacgcga cgcggcttcc cgcactcacc atcgcggccg tcgaggtcga
     8041 tggcggagag acgatatcgc acgacacggt ccgcacccgc gtcgaaaacg tcctctccgg
     8101 ctcgtacgtg ctcctcgttc ccaagcggtt ctcgtacctc tacccgcacg acgcgatcgt
     8161 cgcgtcgctt tcagcggttc ctcgcgtgca cgacgtgtcg gtcgaacgca cgtctcgaaa
     8221 cgagctcgcc gtgtccttcg atgagtacgt accccatgcg ctctggtgcg cgagcggagc
     8281 atcctcgacg ccgtgctggt tcatcgacga caccgggtac gccttcagcg cggcgccaga
     8341 ccttaggggc gggacgctcg ttcgacatct cgacgagaac gcgaccgacg tccacgaggg
     8401 acaggtcatc gacgctgcga agctcgccgc ggtcgatcgc ttcatctcgc gcgcggaacg
     8461 ggaactgcac tttcgcatca ccgcgctcac gtacagaaaa gacggcgacg tcgagttctc
     8521 ggtaaacggc ggcggcatga tactcgcctc cgccgataag gacctcgatg cggcgttcga
     8581 taacctaaaa acggtcctcg agtcgccgaa gttctcgcac ctcgctcccg gcacgttcaa
     8641 gtacgtcgac gtccgccttg agagcaagat cttcgtgaac gagacgactg ataccgacga
     8701 gaaacttccc gagtagcgtg attcgtggta tcacatcacg catgtcattt tgggattcat
     8761 cgccgcggcc gctcatggta ctcgcgccac tcgcgaacgt cactgacgcg gcctttcggc
     8821 gcatcatcgc gaagtactcg gcgcataggc gcaaggacgg cacggtcggc ggaccggacg
     8881 tcatgtggac cgagttcgtg tcggccgacg gcctcgttcg tgcgacacca gaaggacgag
     8941 cgaaactcat ggcggacctc atctataccg aggccgagcg accgatcgtc gcgcaactct
     9001 tctcgtcgaa tcccgagcat atggagcgct cagctcggct ctgtcgtgag ctcggcttcg
     9061 acggcgtcga catcaacatg gggtgtcccg accgcaccgt cgagaagcag ggggcgggtg
     9121 cggcgatgat caaacacccg gaggccgcgc gcgaggtcat ccgcgcggcg aagcggggag
     9181 cgggcgagct gccggtgtcg gtgaagacgc gcgtcggcta taacaagata gagctcgaga
     9241 cgtggatacc cgcgcttctt gaggagcggc cggccgcgct cacgcttcac gcgcgaacgc
     9301 gaaaggagat gtcgaaggtg cccgcgcggt gggagcacgt ccgcgaggcg gtcgccattc
     9361 gggacgcgat gcagggaggt tcggagcgca cgctcattct cgggaacggc gacgcgtcga
     9421 gcctcgcgga cgcggaagcg aaggtgcgtg agacgggagc ggacggcgtc atgttcggac
     9481 gggccatctt cgggaatcct tggttcttcg atccttcggt cgacgtagat gcgcttccgc
     9541 tctccgagcg catgcgggtc atgctcgagc acacacgtct tttcgaggaa ctcctcccgc
     9601 acaagaactt cgcgctcatg aagaagcact acaaggcata cgtcaataac ttcgacggtg
     9661 cgaaggaact gcgtgcgacc cttatggagc tcgactcgtc cgacgcgatc gaagccgcgg
     9721 tctccgcgtg gctcgccgag cgcggtctct cgctttagcc ttcggttttc aacacgtacc
     9781 cgcgtcttca tattttctct acgtacccct ctgctacaat gaggaggtaa tgaacgatgt
     9841 actctacacg aacctctttt tctccatcac ggcgctcgcc gtcgtcgtct tcaccgtctt
     9901 tctctgcatc gccgcgtacc acgtgatcaa ggcgctccgc gtgctccgca ggatactcga
     9961 ccgcatcgac gccggcgcgg atgtcatcgc gggcgatgtg cggagcgttc gcgccttctt
    10021 cgcggaaggc gggttcttgc ggcgcctcgt ctccctcctc accggacggg aaggtcggag
    10081 ccgcgagcac aggacgcgag agcgacggaa gcaagattta aaaactagcg acgaatagaa
    10141 atagcatggc gaagaaaacg aaagaatcga tgagcacggc gcagaaggta ggtctcggtc
    10201 ttggtctcac cgcggctgca gtgaccgccg ctggcgcata cttcctctac gggacgaagg
    10261 aatcggcgca gaatcggaaa cgcgtgaaag gatgggtcct caaggcgaaa ggggacgtcc
    10321 tcgaagcgct cgagaaggcg aagacgataa cggagaagga gtacaacgac ctcgtcgacg
    10381 ccgcgacgaa ggcgtacggt acggtaagca aggcgagcca gggagaggtc cgcgatttca
    10441 agaaggagat gcagggacat tggctcgagc tccagaagag caaggccgtg aagaagctca
    10501 tgtcggacgt gaagccggcg aagaagaccg cacggcgaaa gacccccgcg aagaagacga
    10561 agtaacgaga acagacgacg ctaccgcacc agccccctgg tgcggtatgc tatttccatg
    10621 aaccgcagga tcatcctcta tagtctcttc ctcatggcca tcttcggtct cgcggccctc
    10681 gtcgcgttcg tcggaccgca cctcggtatc atgcgtgggg gactgaccgc cgaggtgagc
    10741 gctcccgccg ggcttacgac cgatacgtgg ggtattttcg atccagcgac gggtgagctc
    10801 atcgccggag aacacgcgtc cgacgcgcgg ccgatcgctt cggtcgcgaa gctcttcacc
    10861 gcgtatgcgg tcatggcgag cgagaagcga gacgatctcg ttacgatatc cgcgggcgac
    10921 gtcgcgaccg agggtcgcgc ggggaagctc tacgtcggcg agcgcatgac gccctacaag
    10981 cttctcttcc cgatgctcat cgaatcgtcg aacgacgcgg cagaggccgc caggcgaacg
    11041 ctcggcgtcg atttcacgaa gaacgtcacg aacatcgagt cggacctcgg cctcaagaac
    11101 acgacgctcg ccgatggtgc gggtcttagc cccaagagcg tctcgtcagt cgaagatctc
    11161 tcgcgcttct acgcgcacct caagaagacg tacccgcacg tgctcgatat cacgcggctc
    11221 aacatgtaca tcgacgacca tacggggtac gtgaacaacg acccggcgcg ttcgctcacg
    11281 acgttcgagg gcggcaagaa cggatacacc gacgaggcgg cgaggacctt cgtcggcacg
    11341 ttctcgaagg acggcgccga ggtcggcatc gtcctcttgg ggagtactga catagtgcgg
    11401 gacatcgaac gcctcctcgc gttccgaaag agcccgaagg ggggcttctg atatactgta
    11461 cgcatgtccg gcatccagct cgcgctttat aggaagtatc ggcctacctc gttcgatgag
    11521 gtgcggaacc aagagcacgt catctccgtt ctcaaaggcg cgatagagaa gaagcaggtg
    11581 cctcatgcgc tcctcttcac cggcccgcgc ggcacgggaa agacgaccgt cgcgcgcatc
    11641 ttcgcgaagg cggtcggcgc atcggacgtc gatacgtatg agatagacgc cgcatcgaat
    11701 cggagcatcg acgacgttcg cgagctgaag gaagcggtga gcacgctccc gtacgagtcg
    11761 ccgtacaagg tgtacatcgt cgacgaggtt catatgctca cgaaggaggc gttcaacgcg
    11821 ctccttaaaa cgctcgagga gccgcccgcg cacgtcattt tcatactcgc gacgacggag
    11881 cgcgataagc tcctcgatac catcacgtcg cgctgtcagg ttttcgaatt ccacgcaccg
    11941 acgcgagagg agcttcgcga gaccgttatc gcggtcgcga agcgggagaa gttcaagctc
    12001 gcgccacacg tcgctgacgt catcgcgctt tcggccgatg gttcctaccg ggacgccctc
    12061 ggtatcaccc agaaggtgat gctcgcctcg ggagacaggg agctcggcga agacgaggtc
    12121 gcacgcatcg tcggcgcacc gcgaagcgcc gtccttagcg aggtcgtcgc cgcactcgcg
    12181 tccaaagacg ccgatcgcgg actccgcgcg ctttcggagg ccgagacggc acacgtcgac
    12241 atgaagttct tcttgaagct ccttctcgag cgtctccgcg ccgtcgtcct ccttaggaac
    12301 acgaaggaca aggacgcgct cctttcgcgc ttcggcgatg aggaggggaa gacgctcctc
    12361 tcgtacgcga acgacgcgaa gtcgcccatc aactcgcacc ttctccttcg cgcgatcgag
    12421 ctcgggggat acgtggggaa gagcttcgcg ccgacccttc ccatcgaact cttgctcatc
    12481 gaacaacacg cctagccatg cacgtatatt tcgttagaca cggcgagacg acgatgaatc
    12541 gacggctcac gcaccagcca cccggcacgc cgctctccga aagggggcga gagcaggcgc
    12601 gcgaggtcgc ggagttccta cgctcggtga accccgacct ccttctcacg agcgactaca
    12661 cgcgagccct cgagaccgcc cgcatcatcg gcctctccgt ggggctcacg ccgcgcattc
    12721 ccggcttcct gcacgagctc gtgcggcctt cccggctctt ccaccgttcg cacttcgcgc
    12781 cgtgcacgat ctggtattct ttcctgaccg cgcttcatcg taacgacctt tcctggcact
    12841 tcgaggatgc ggagaacttc ccggagctcg ccggcagaac gcgtcgcgcg ctccagtatc
    12901 tcgagtcgct ctccgcgacg catcgttcgg tcgtcgtcgt ctcgcatacc gtcttcataa
    12961 acgtactcgt cgcgtacctc tgccgcgagc gcatgctcga tgtcgtcgac ctcttccggg
    13021 tgttcttcca tttcgagcgc ctcaagaacg gcggtgtcgt ccacgtcgag ttcagcggac
    13081 gacgcgagcg gggaacgtgt ccctggcaac tcgttcccat ggcacccgac gcgctataat
    13141 cgacgggtat ggaacgacgg cacgcgatac gacgaggtaa gagaacgatg acggtcgtcg
    13201 ccgtctcgct cctctctttc gccttcgtgc tctggggcgt cgcgactttc atgctcggag
    13261 gcacgaacgt tcggtacgaa ggtgtcaacg catcgctcac gaacgcgtcg gcatttcccg
    13321 tgggcgtcga cccccgtgaa aagcgcatcg tcgaagtccc taacctcgac gcgtacatcg
    13381 aacgggaagg cttcgcagcg acgcgcgcct cgtctcgcca gctcacctgg gtcagccgag
    13441 tcatgggtca gctcgcgctc atggattggt tccaaaacct cgcgtccctc tcatcacgga
    13501 cgctcgtcat agaaccgggc gagcgcggcg aggaggtcgc cgaccacttc gcgaagatac
    13561 tcggttggag cgaggatgag cgtgcggcgt tcgtgcgcgc ggtcgcacag cggacacccg
    13621 ccataagaga ggggaagttc tatccgggtc gctacaccgt cccgcgagac gcatcgcccg
    13681 aggccgtcgc ggacgccgta ctcggttcgt tcaccgagaa cgtgctctcg cgctacgcga
    13741 cgtcgaccgc cgccgccgtg ccgcttcagg acgcgcttac catcgcttcg ctccttgagc
    13801 gcgaagcata cgacttcgac gacatgcgcc tcatcgccgg cgtcatctgg aatcggctct
    13861 tcatcggcat gaacctccag atagacgcga ccttgcagta cgcgaagggc acgaaacctt
    13921 cagaaccgtg gtggccgcag gtgggaccgg ccgataagta catagaatcc gcgtacaaca
    13981 cgtacgagaa tcccggtctg ccgcctgcgc cgatcgcgaa tccctccgcg gaggcgatac
    14041 tcgccgcgct caatccaaag aacaccgact gcctctacta tttccatgat aaaaaggccg
    14101 gctttcattg cgcggcgacg tacgacgagc acgtcgacct cctgaagcga tactacggca
    14161 ggggaaagtg aaatctagta tactggagcc catgctcggt gctctcgaat cgatggtcga
    14221 atccctcgcg gcggtggtcc ccctcgaggt cttcgtcacc gtcgcgtcta tactcgagga
    14281 agttctcgca ccgataccgt cgccgaccgt catgatgctc acgggcgccc tcgcctccgt
    14341 ccaggaccgc acgctcctca tgctcgtgcc gctcctcctt ctcggtacca tcgggaagac
    14401 gctcggggcg agcgtcgtct acgtgatcgc ggaccgcggc gaagacctca tcatcggccg
    14461 cttcggcagg tacttcgggg taacgcgtga ggatgtggag gccttcgggg atgaactcgg
    14521 caccggcgcg cgcggatact ttctcctcac gctcttccgc gcactccccg tcgtcccatc
    14581 gacgctcgta tcggtcgggt caggcctcat caaggtgccg taccgtatgt tcctcatcgc
    14641 gacgctcgtg ggtaccatcg tccgcgatgg attctatctc tacgcgggat acatcggcac
    14701 ggagacgttc gccgccatgg tgaaccactc cgcgagcgcg gagacgtaca tcgaggcggc
    14761 ggcgctcgtc gtcgtattcg tgtacatcgc ctatcgactc atgaggcaga agcgccgtta
    14821 acgctctggt ctctggaaag tccctctggc aggggaacat ggcacacgct cacttcgtag
    14881 catcgcgtgc cgtcttgagg gcgcgtgtcc accaaaggag ctcctcgagc atgtggtccg
    14941 caggcgcctg aagcgatgcg aagagctcgt tcgccggcgc tttgccagtc ttcacggggt
    15001 agaaacgttc accagtgat
//
LOCUS       DASTSP010000013         6157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1817279, whole genome shotgun sequence.
ACCESSION   DASTSP010000013 DASTSP010000000
VERSION     DASTSP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817279"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..951)
                     /locus_tag="VFS75_00565"
     CDS             complement(181..951)
                     /locus_tag="VFS75_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4677195.1"
                     /translation="MDKSKYINKVKGHALIDGGSGGIGSECVRGLVANGAEAITITFN
                     SNEAKARKVATEIEAEGVRVYVDRANPLDVTEYAAFLERAVAFHGGMDISTYVNAVGW
                     SPNTPIEEQTVEEHRKVMDINLHGPFFATRTNLVRMRTMGIRGSVVLITSTNGINSNS
                     PISSHYDASKAGLVPHIRNFGTWFAPHGMRVNGVAPGWIETPMNDTLPLEERERESAK
                     IWIGRWAEPHEVANMVVAVAGSTGSYVTGTNIIVDGGY"
     gene            1094..2014
                     /locus_tag="VFS75_00570"
     CDS             1094..2014
                     /locus_tag="VFS75_00570"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677196.1"
                     /translation="MCIILLSPMLATAKDLNVDFVQGVWFSQEPVFVGVPTRVYVAFR
                     NNTESDLTGTIRFLDNGKQMGSSDISVLRGRIVEAWTDWTPTTGNHTITVTMANAALH
                     EIGKEPAPIDIEGIATEDAVTVDYDTDGDGIGNASDTDDDGDGISDIDEKARGSDPLT
                     PNPTPVATTTTSAPLASTTAPAGLEEFLPDGKTSDALRSVTDGLTNVKSALDAYRRER
                     NAQLADVSTTTPLDTATITRSKIEGQKTFLGGLVAGTAYLLKNLYTFALYALSRALTY
                     PAMVELAVLLGLLLTIYRFARRVGRRPGWD"
     gene            complement(2053..2403)
                     /locus_tag="VFS75_00575"
     CDS             complement(2053..2403)
                     /locus_tag="VFS75_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677197.1"
                     /translation="MAKSYLTREELEAYSTVEDLLYQSASCDLANWGLCTNGVESMFR
                     TENDEWKLHLPLAKIIQSTECWCEAVGHGNVFVIPVVEVKSGTLWCMYVSAAPGERYE
                     PGPRIAEAPSLLPN"
     gene            2521..2871
                     /locus_tag="VFS75_00580"
     CDS             2521..2871
                     /locus_tag="VFS75_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU4677198.1"
                     /translation="MNTYTGSCHCGAVKFTVDADIEKIMECNCSHCARKGFLLSFVPA
                     ENVTIESGEDNLTEYRFNKKHIAHLFCKTCGVQAFGRGERPDGSTTYAINVRTIPDLD
                     VTALTIEKVDGKNF"
     gene            complement(2897..3499)
                     /locus_tag="VFS75_00585"
     CDS             complement(2897..3499)
                     /locus_tag="VFS75_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677199.1"
                     /translation="MVPWKIVVLSGASGSGKTTCIRVLESFAPAVRFCQLPSVTTRAP
                     RPGDEPWYEYVDLDTFRKMKSDGKFLWIEAVHGNLYGTTKDAFETALAYGDCLLASLT
                     PHDIPTIAMRGLDYGVPLEEMLFLYIRHPGDEVLRKRLEARGDAKDVIERRIADCANW
                     DGLAKDAKLVFPRQVKLDVTFIENDTSEETFAGKVRTLFT"
     gene            complement(3575..3784)
                     /locus_tag="VFS75_00590"
     CDS             complement(3575..3784)
                     /locus_tag="VFS75_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677200.1"
                     /translation="MPRRKYTLWDPPPAHLRTPLRASAKLTKRELAALKKDIEQALEE
                     EKRKSPWLGHEASQRYPLRRYDAAC"
     gene            complement(3969..4442)
                     /gene="rpsG"
                     /locus_tag="VFS75_00595"
     CDS             complement(3969..4442)
                     /gene="rpsG"
                     /locus_tag="VFS75_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU4677201.1"
                     /translation="MRRPIKRRAPLTKDERYNSQNVTRLINYIMLRGQKETARKIVYA
                     AFEEIKNAKEGGGDPAEIFETALRNAGPQMEVRSRRVGGANYQVPREVRPERRLALAL
                     RWIITAARGQKGTPMFKALAAEIMAAAREEGAAVKKKEDTHKMAEANRAFAHFAW"
     gene            complement(4492..4902)
                     /gene="rpsL"
                     /locus_tag="VFS75_00600"
     CDS             complement(4492..4902)
                     /gene="rpsL"
                     /locus_tag="VFS75_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017815358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU4677202.1"
                     /translation="MATINQLTKRGRRGGAGRTKTGALRSGFNSIKNRPNSYNSPFKR
                     GVCTRVTTKTPRKPNSAIRKIARVRLTNHEEITAYIPGIGHTLQEHSVVLVRGGRVKD
                     IGVNYTIVRGKYDATGVDKRMRGRSRYGAKKSKK"
     gene            complement(5220..5849)
                     /locus_tag="VFS75_00605"
     CDS             complement(5220..5849)
                     /locus_tag="VFS75_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4677203.1"
                     /translation="MFELITIAILILSIVFHEVAHGYAANWLGDPTARLAGRLTPNPV
                     VHIDPLGSVIIPAFLFLSHAPFLFGWAKPVPYNPYNLRNGRWSESIVAAAGPLANLFL
                     ALVFALLIRFAVPLHLSGPFIEMSSYIVFINVLLGLFNAIPLPPLDGSKIIAAFFPGS
                     LEYRYRALVGAVERIGLLGLFLMVFIFMQVLWQPFSALVTVVFRVLTGL"
     gene            5970..>6157
                     /gene="rpmB"
                     /locus_tag="VFS75_00610"
     CDS             5970..>6157
                     /gene="rpmB"
                     /locus_tag="VFS75_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU4677204.1"
                     /translation="MAKQCDITGKKAQVGGRYSNRTRATQFNPSGKVLRQPNLQKKRI
                     FVPEIGKTVVVNVSTKGLR"
BASE COUNT         1301 a         1819 c         1774 g         1263 t
ORIGIN      
        1 tgcttcggtt cacggagcac atgctcgaac cgtacccacc cgagatcgaa gcgtcgatcg
       61 cgctctatcg gagcatgcga aaggcgtgat gacaatcgtc gaggacccgc cgcagcgatg
      121 cgacgggtct ttttataaaa aagaccgtgt gccgaagcac acggtcccga atcgtactcc
      181 ttagtatcct ccgtcgacaa tgatgttcgt tccggtgacg tagctaccgg tcgaaccggc
      241 gaccgctacg accatgttcg caacttcatg cggctctgcc cagcgtccga tccagatctt
      301 cgccgattct cgctcgcgtt cctctagcgg cagtgtgtcg ttcatgggcg tttcgatcca
      361 tcccggagcg accccattca cacgcatgcc gtgaggcgcg aaccaagtgc caaagttccg
      421 aatatgcggg acgaggccgg ctttcgatgc atcgtagtgc gacgagatcg gagaatttga
      481 gttgatgcca ttcgtcgacg taatgaggac gaccgaccct ctgatgccca tagtgcgcat
      541 gcgaactaag ttcgttcggg tggcgaagaa cggcccgtgg aggttgatgt ccatcacctt
      601 cctgtgctcc tccacggtct gctcctcgat cggggtgttc ggcgaccagc cgacggcgtt
      661 cacgtacgtc gagatatcca tcccgccatg gaacgcgact gcgcgttcaa ggaaggcagc
      721 atactccgtg acatcgagag gattcgcgcg atcaacgtac acgcgcaccc cctccgcctc
      781 gatctcggtg gcgacttttc tcgccttcgc ctcattacta ttgaacgtga tcgtgatcgc
      841 ctcggcgccg ttcgcgacga ggccacggac gcactcgctc ccgatgccgc ctgagccgcc
      901 gtcgatgagc gcatggccct ttactttgtt gatgtacttg gatttgtcca taagagcttt
      961 cagagtttga tctgataaaa gaataccacg agaaatgagt cacgtcaatt ttctacttct
     1021 cacagacctt ggatttccgg gccgtttcga acggtatact acatactata tgaagcggct
     1081 ccttattgcg caaatgtgca taatcctcct ctccccgatg ctcgctaccg cgaaggacct
     1141 aaacgtcgac ttcgtccagg gtgtctggtt ctcacaagaa cccgtcttcg tcggcgtgcc
     1201 gacgcgcgtc tacgtcgcgt ttcggaacaa caccgagagc gacctcacgg gaaccatccg
     1261 ctttttggac aatggtaagc agatgggctc ttccgatatc agcgttcttc gtggacgtat
     1321 cgtcgaggcg tggaccgatt ggacgccgac gacgggtaac cacacgataa ccgtgacgat
     1381 ggcgaacgcg gccttacacg agataggcaa agaaccggcg cccatcgaca tagagggcat
     1441 cgcgaccgag gacgcggtca cggtggacta cgataccgat ggcgacggta tcggaaacgc
     1501 gtccgacacc gatgacgatg gcgacggtat ctcggacatc gatgagaaag cccgcgggag
     1561 cgacccgctc acgccgaacc cgacgcccgt agcgactacg acgacatccg caccgctcgc
     1621 ttcgacgacg gcaccggccg gcctcgagga gttccttccc gacggaaaga cgagcgacgc
     1681 gctgcggagc gtgaccgacg gcctcacgaa cgtgaaaagc gcgctcgacg cgtatcgaag
     1741 agaacggaac gcacagctcg cggacgtctc gacgacgact cccctcgata ccgcgaccat
     1801 cacgcgaagc aagatagagg ggcaaaagac gttcctcggc gggctcgtcg cgggaaccgc
     1861 gtacctcctc aagaacctct atacgttcgc gctctatgcc ctatcgcgcg cgctcaccta
     1921 ccccgcgatg gtcgagctcg cggtgctcct tgggcttctc cttaccatct atcgattcgc
     1981 gaggcgcgtc ggccgccgtc ctgggtggga ttgaaaaacc gctcgcgtac tgcgagcggt
     2041 ttgaactaag tttcagttcg gaaggagcga cggtgcctct gcgatgcgag gtcccggctc
     2101 gtatctctcg ccaggagccg cggatacgta catgcaccac agcgtgccgg acttcacttc
     2161 gacgaccgga atgacgaaga cgttgccgtg acccacggcc tcgcaccagc actcggtcga
     2221 ttggattatc ttagcgagcg gcaagtgcag tttccactcg tcattttcgg tacggaacat
     2281 gctctcgacc ccgttcgtgc atagtcccca gttcgccaga tcgcacgacg cgctctggta
     2341 taggagatct tccaccgtac tgtacgcctc taactcttcc cgcgtgaggt atgacttagc
     2401 catttgggca ccctccgata agaagtgtcg ggactcggga ctccttcacc atagtaagcc
     2461 gtctaagggc cgatggccaa cttcacgagg tgattatcga gacatgctac aatcgggcgc
     2521 atgaatacat atacgggaag ctgtcactgc ggcgctgtca agttcaccgt cgacgcggat
     2581 atagagaaga tcatggagtg taactgctcc cattgcgcgc ggaagggatt ccttctttca
     2641 ttcgtacccg cggagaacgt cacgatcgag tccggcgagg ataaccttac cgagtaccga
     2701 ttcaataaga agcacatagc gcatctcttc tgcaagacgt gcggcgtgca ggcgttcggc
     2761 cgcggagagc gaccagacgg aagcacgacg tacgcgatca acgtacgcac tattcccgac
     2821 ctcgatgtca ccgcgctcac catcgagaag gtcgacggaa agaattttta aaaaaaagga
     2881 ggcgcaagcc tccttttcaa gtgaagagcg ttcgcacttt ccccgcgaac gtctcctcgc
     2941 tcgtatcgtt ctcgatgaac gtgacgtcga gcttcacctg ccgtgggaag acgagcttcg
     3001 cgtcctttgc gagcccatcc caattcgcgc agtcggcgat cctccgctcg atcacgtcct
     3061 tcgcgtcgcc acgcgcttcg agccgcttcc ggagcacctc gtctccgggg tgtcggatgt
     3121 agaggaagag catttcctcg agcggcacgc catagtcgag accccgcatc gcgatcgtcg
     3181 ggatgtcgtg cggcgtgagc gacgcgagga ggcagtcgcc atatgcgagc gccgtctcga
     3241 acgcgtcctt cgtcgtgccg tagagattcc cgtggaccgc ttctatccag agaaatttcc
     3301 cgtcagactt cattttacgg aacgtgtcca aatcgacgta ctcgtaccac ggttcgtcac
     3361 caggccgcgg agcccgggtc gtcacgctcg gaagctggca gaagcggacc gcgggagcga
     3421 aactctccaa aacgcgtata catgtcgtct tcccggagcc agaggcgccg gagagaacga
     3481 ctattttcca gggcaccatg gccatctcct aaagaacaag attggcgcaa ctatacaacg
     3541 taaacggccg tccgcaagtg cggacggccg ggacttaaca ggcggcatcg tatcttcgga
     3601 gaggataccg ctgactcgct tcatgcccga gccaagggct ctttctcttc tcttcctcga
     3661 gcgcctgctc gatgtccttc ttcaaggccg cgagctcgcg tttcgtgagc ttcgcgctgg
     3721 cacgaagcgg ggtgcgaaga tgtgccggtg gcggatccca aagcgtgtac tttcgacgcg
     3781 gcatgacgtt ctctccttgg ttgatttcta cccctagcgt agcatagaaa aacaaaacag
     3841 ccgctggtgc ggctgtttta tttcgtatta aagtgaccgt tcgactaggg cgtcgattca
     3901 aggcgccacg taggcgcatg aatcgacgga ctccgaaaat atagtcgctt gcgctcctta
     3961 ttctcgggtc accacgcgaa gtgtgcaaag gcgcggttcg cctccgccat cttgtgcgtg
     4021 tcctccttct tcttcaccgc agcaccctct tcgcgagcgg ctgccatgat ctctgcggca
     4081 agcgccttga acatcggcgt acccttctgg ccgcgagcgg ccgtgatgat ccaccggagc
     4141 gcgagcgcga gccgacgctc gggccgaact tcgcgcggga cctggtagtt cgcgccgccg
     4201 acgcgtcgtg agcggacctc catctgaggt cccgcgtttc gaagtgcggt ctcgaagatc
     4261 tccgcggggt cgccgcctcc ctccttcgcg ttcttgatct cctcgaacgc cgcgtagacg
     4321 atctttcgcg cggtctcctt ctggccgcga agcattatgt aattgatgag tctcgttacg
     4381 ttctgcgagt tgtaccgctc gtccttcgta agcggagctc gtcgtttgat tggtcgtcgc
     4441 atatatttcc tgtcgttatc tatagcgagt atagtctatt tgtaatttag tttatttctt
     4501 cgacttcttc gcaccgtagc gtgagcgtcc tcgcatacgc ttgtcgacgc cggtcgcgtc
     4561 gtacttaccg cggacgatcg tgtagttcac gccgatgtcc ttcacgcggc cgccgcgtac
     4621 gaggacgacc gagtgctcct ggagcgtgtg tccgatgccc gggatgtagg cggtgatctc
     4681 ctcatggttc gtgagacgaa cgcgagcgat ctttcgaata gcggagttcg gcttgcgcgg
     4741 cgtcttcgtc gtcacgcgcg tgcagacgcc acgcttgaac ggcgagttgt acgagttcgg
     4801 acggttcttg atcgagttga aacccgaacg gagggcgccc gtcttcgtgc gccccgcgcc
     4861 gccgcgtcgt ccccgcttgg ttagctgatt gatcgttgcc atgatgaatt aaatccctga
     4921 aatagtgggc ctactctact atatattgag aaattcgcaa gcagtccggc cgtgagcgtc
     4981 ccgaaaataa aaaaatggca gagtctcgcc gactacaacg tcgaccctgc catctgggca
     5041 tagctcggaa tgttcgtctg tccgcaaccg ttcgcttctg ggagtgaagc ggggtcgcga
     5101 tagactagcg cccgagagca cggtcccgac cggttcccaa aatcattaac taagcgatac
     5161 cagtaaaaat aaatttagtc aaggtactgc ggcgccacgt cgcgcgcata tgccccaatt
     5221 tacaaaccgg tcaaaacacg aaagacgacc gtgacgagcg cggaaaacgg ctgccaaagg
     5281 acctgcatga agatgaacac catgaggaaa agaccgagaa gtccgatgcg ttctaccgcg
     5341 ccgacgagcg cgcggtaccg gtactctagc gaccccggaa agaaggcggc gatgatcttc
     5401 gacccgtcga gtggcgggag cggtatcgca ttgaagaggc cgaggaggac gttaatgaag
     5461 acgatgtagc tcgacatctc gatgaacggt ccggagaggt ggagcgggac ggcgaagcgg
     5521 atgagaagcg cgaagacgag cgcgaggaag aggttcgcga gaggtccggc cgcggcgacg
     5581 atcgattccg accagcggcc gtttctgaga ttgtacgggt tatacggcac gggcttcgcc
     5641 cacccgaaga gaaacggcgc gtgcgagaga aagagaaatg ccggaatgat gaccgaaccc
     5701 aatgggtcga tatggacgac cgggttcggc gtgagccggc cggcgaggcg cgccgtcggg
     5761 tcgccgagcc aattcgccgc gtacccgtgc gccacttcat ggaacacgat ggagaggatg
     5821 agaatggcga tggtgatgag ctcgaacata tatatatgat agtagcacgt attataggcc
     5881 gggaagatga aagctcaaac ggcacctcgc catctcgtgt tgcaatttga ccgttccatg
     5941 ctagtatagc cgcactaata taacgacgta tggcaaagca gtgcgacata accggtaaga
     6001 aggcgcaggt cggcgggcgg tactcgaacc gaacgcgtgc gacgcagttc aatccctcgg
     6061 ggaaggtgct tcgtcagccg aacctccaga agaagcgcat cttcgtaccg gagatcggca
     6121 agaccgtcgt cgtgaacgtc tcgacgaagg gcctccg
//
LOCUS       DASTSP010000014        10620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_35031, whole genome shotgun sequence.
ACCESSION   DASTSP010000014 DASTSP010000000
VERSION     DASTSP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35031"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..917)
                     /gene="atpG"
                     /locus_tag="VFS75_00615"
     CDS             complement(6..917)
                     /gene="atpG"
                     /locus_tag="VFS75_00615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU4677205.1"
                     /translation="MALKQIKNKIKATERTSKVTKAMQAVSAVKMRKSQERALAGRPF
                     AEAALRILEHVSRDYGAQSHPLFAEHAEGKHAVVVVTSDKGLAGSLNSSVLKEAERVV
                     AASSKENVAVICFGRKSYEHFSRRGYEVPLHFLNVQDDVSLDDFEVVASHLISEFSAK
                     RYHAVHVVYESFISTFAQEAVSERILPLKSASLQEIVAGITPSAGKWSGTAEERPHIP
                     YTVEPSGEEVYAEIFPLLIKVMLYHALLESKASEHSARMVAMKNATDKAEEVERTLTL
                     AYNKERQSVITAEVSEITGGIEAMKAA"
     gene            complement(924..2483)
                     /gene="atpA"
                     /locus_tag="VFS75_00620"
     CDS             complement(924..2483)
                     /gene="atpA"
                     /locus_tag="VFS75_00620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU4677206.1"
                     /translation="MSTIIERIKKELEAIAPEREALEIGIVVAVGDGVAEIEGVKEAR
                     MMEMVLFEDAEGTALKDAMEETGSLFGLVLNLEEESVKVVILGDASRVTEGMTVRRTG
                     KLLSIPVGAGIVGRVVSPLGEVIDGKGSYEATRDMPIERVAYGVMERAGVDEPIHTGI
                     KAIDSMIPIGRGQRELIIGDRGTGKTTVAIDTIINQKYEPKENRPICIYVAVGQKESK
                     TARIVRELEENGALEYTVVVATSAADPSPLQFLAPFAGAAIGEFFMEDGKDALIVYDD
                     LSKHAVAYRQMSLLLRRPPGREAYPGDIFYLHSRLLERAAKLSDALGGGSLTALPIIE
                     TQEGDVSAYIPTNVISITDGQIFLETDLFNKGIRPALNVGISVSRVGSAAQTKAMKKV
                     SGKIRLELAQFRELEAFMQFASDLDENTKKQIESGRKYTELLKQDQHATMPFEEEVVS
                     IFAANGGILDGASVEKVREIERTFLEQLERDTPDILETIRKERALSDETTAKLQAIVD
                     EFKKTHPNLYA"
     gene            complement(2495..2884)
                     /locus_tag="VFS75_00625"
     CDS             complement(2495..2884)
                     /locus_tag="VFS75_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677207.1"
                     /translation="MYVRDYVKAAYDVIEREGNVDKTLSALRTYLDGRGLAPMYPRIL
                     RGLAEEFRRRERTSRPVLSVARERDVARYEREIADAFARLGSSEKPAVEVNDNLIGGF
                     VAEASGKRIDRSFKTKLLHAYQRLTAR"
     gene            complement(2893..3345)
                     /gene="atpF"
                     /locus_tag="VFS75_00630"
     CDS             complement(2893..3345)
                     /gene="atpF"
                     /locus_tag="VFS75_00630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU4677208.1"
                     /translation="MQELIKAFGIDWRLIVIQVFNFGVLVGILWYFLYTPVLKMLSDR
                     ASKIARGVKDAEDAAEARKNADVEKHRVLEDAHREASDIVKRGTEHAEEKGKELLNEA
                     QERIARELESAKLAAAELKAQAQKESEAEIAKLAILGAERVLRDELSK"
     gene            complement(3358..3579)
                     /gene="atpE"
                     /locus_tag="VFS75_00635"
     CDS             complement(3358..3579)
                     /gene="atpE"
                     /locus_tag="VFS75_00635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU4677209.1"
                     /translation="MEAIKVLGMALAVGLGMIGPGIGIGILVSKALEAIGRNPEVSGR
                     IQTNMFIGIAFTEALAIFAIVVGFIIRFG"
     gene            complement(3667..4440)
                     /gene="atpB"
                     /locus_tag="VFS75_00640"
     CDS             complement(3667..4440)
                     /gene="atpB"
                     /locus_tag="VFS75_00640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU4677210.1"
                     /translation="MNTAHAASEGIAIHLSAPVLGHFFGIPLTATLLTTWVMIALLLV
                     FAIVAKRRLAMVPSKTQVAVELFVGGAFDYVADALEDRELAKRFFPLIMSIFVFIFAL
                     NVIALLPGVDALGMWKIEDGLSEFVPFLHSANTDLNIPIALALIAFFTVEVTGIAFLG
                     VWKYGSKFINFASPLAFVIGIIELFSELARLVSFSFRLFGNIFAGKTLILIAIFFVPF
                     ILPVPILAFELFVGFIQAFIFAVLTLFFIKLAIAEPEHH"
     gene            4639..4712
                     /locus_tag="VFS75_00645"
     tRNA            4639..4712
                     /locus_tag="VFS75_00645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4673..4675,aa:Ala,seq:tgc)
     gene            complement(5032..7575)
                     /locus_tag="VFS75_00650"
     CDS             complement(5032..7575)
                     /locus_tag="VFS75_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677211.1"
                     /translation="MNASTTLRIASPIVIMSLLLALLPVATVRADDGTTTPPVEAGLA
                     NDTVPLTEAVVSSDPEPSDDAVTVATSSAPSGDATSTETVSADAAAMESGTSTSALSS
                     STTPVAHLTATTSASSTTWSSTGTSSATSTEWNGLGSTTVDTDNGGAGTTTVIKTGTA
                     VALANVLNLVNSNFVNSKGVVLFKNFFDTLTGGLDLRDYLAASSSTCSLSGCADNEVH
                     TNIENGAILDNSLYVTAETGGNTIDGAENAAIASGDAYAGVNLVNVANTNVIDSNYLL
                     VTMNAFQDVNGDIVFPSLEKFFGELSQSTTPAALSITNEGSIDNDISLDTATGANMAD
                     ASSSTISSGASASGVNVFNQLNTSLVGGKSVSIIFRVNGSWAGQVFGAPDTLAWMDNG
                     DGGIYLFDTEGGTGGGGSLYATTTNAASIKNRVHVVALTGENEIHNADTALISTGNAY
                     AGANIMNVANATVVGRNWILAIVNILGDFNGNIAFGRPDLWVGGQATVPTGFGNGDTA
                     AYKVTVINNGDSTATDVTLRATYDADHLALSNASVPLSLDGRDAVWHLGDIPAGGAVE
                     LTYSGTVKNAGEGTDIDTTYDVMEHETDNNMVDNSDIVTVSTAVHHHGSNSHGKKPEP
                     TTLAGGSVTVPRALVVERESATTSVRLDVAATTTERLTLTNDGDEAVDGVVLHDVLRD
                     PEGAVLHEEVWDLGTVAAHETVRIGYDIGFDVDAPVGVYRLETVLDAAGMTRTYGMNG
                     SILVWSSPAHIPAVPLVPAPMTTPSTTEPSVATTTDAASIIDAFLPNIAEAASDHPEN
                     PEVAYIRNLKRELALAGALLVISGSILLMYRYRRDWLDAIRNKIRTAMF"
     gene            complement(7678..8694)
                     /locus_tag="VFS75_00655"
     CDS             complement(7678..8694)
                     /locus_tag="VFS75_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677212.1"
                     /translation="MILHKAASIISTMSLLLAAPVAFADTITVSTTNSASISNNVSVA
                     SNTGGNVSKGAEGARGSMGGSAVGGGDGSAVRGGNGGNGGNGGNGGSITTGDAIGTAD
                     VENDVNSTEVTIEDNRSDVNQYDVTLHARSSVGSSGSESQAANSSYNESSNEQNYANG
                     SDSHETYQSTNSASASEDSSDTDGSWSQASASDSSQSADSGTSNYQQDNESAWQVSSE
                     SNSSDEMAAEQHDNKSIDYTRTYVVAHNDVVVANQNDASVQNSVGVDAYTGFNRSKGA
                     IGAIGDNGANAVGGGDGSSATGGSGGNGGNGGQGGAVVTGVSDSLAKVVTVSGRSLTR
                     ITRN"
     gene            complement(9017..9087)
                     /locus_tag="VFS75_00660"
     tRNA            complement(9017..9087)
                     /locus_tag="VFS75_00660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9053..9055),aa:Pro,seq:ggg)
     gene            complement(9140..9442)
                     /locus_tag="VFS75_00665"
     CDS             complement(9140..9442)
                     /locus_tag="VFS75_00665"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU4677213.1"
                     /translation="MAVRSKQAMKASEVKKRLKNYPDWTVNAATTQIAKTYSFPSFVA
                     GLAFVAKIAVHAEILGHHPDVELSYGKVKVRLSTHDAKGLTPLDFELAERIDSIAP"
     gene            complement(9450..10430)
                     /locus_tag="VFS75_00670"
     CDS             complement(9450..10430)
                     /locus_tag="VFS75_00670"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU4677214.1"
                     /translation="MHRVRVAVVRGGMSDEYEVSMRTGAAVIANLDPERFAPIDVIIL
                     RSGEWLVDGRVRLPEQILYSVDVVFNALHGTFGEDGTFQRLLERYAVPYTGSKVYASN
                     IAMNKILTKRHLRDAGVRFAPHVHVTRESRDDIGRVAERIMRLFGPQYVVKPVASGSS
                     VGTRMVTNPLLLHQALREALDEFEEVMVEQRIKGREATCGVAERYRGHDLYAFPAIEI
                     VPPPHAEYFDATVKYSGETRELCPSTFDMRTKHELERLAKLVHRELGLSQYSRSDFIV
                     APDGVYFLEVNTLPGLTPESLLPKAIEAVGGSYGEFLAHLIDDACRVRVS"
     gene            complement(10506..>10620)
                     /gene="rpsT"
                     /locus_tag="VFS75_00675"
     CDS             complement(10506..>10620)
                     /gene="rpsT"
                     /locus_tag="VFS75_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU4677215.1"
                     /translation="SRAYEALDKAAKRGVIKKNTASRKKSRLMAAIKKAKA"
BASE COUNT         1983 a         3176 c         3200 g         2261 t
ORIGIN      
        1 taatcctacg ccgccttcat cgcctcgatg cctcccgtta tctcggagac ttccgcggtg
       61 atgacgctct gtcgttcttt gttgtacgcg agcgtgagcg tccgctcgac ctcctccgcc
      121 ttgtcggtcg cgttcttcat cgcgaccatg cgagccgaat gttcgctcgc cttcgattcg
      181 aggagcgcgt ggtagagcat gaccttgatg aggagcggga atatctccgc gtatacctcc
      241 tcgcccgatg gctcgacggt gtacgggatg tgcggccgct cttccgcggt tccgctccac
      301 ttacccgccg acggggtgat acccgcgacg atctcctgga gcgacgccga cttcaagggg
      361 aggatacgct ccgataccgc ttcctgtgcg aacgtcgata tgaacgactc gtacacgacg
      421 tggaccgcat ggtaccgctt cgcggagaat tcggagatga ggtgcgatgc gacgacctcg
      481 aagtcgtcga gactcacgtc gtcctgaacg ttgagaaaat ggagcggcac ctcataccca
      541 cgacggctga agtgctcgta gctctttcgc ccgaaacata tgacggccac gttctccttg
      601 gaagacgccg cgacgacccg ctcggcctct ttgaggacgg agctgttgag actacccgcg
      661 aggcctttgt ccgaggtgac gacgacgacc gcgtgcttcc cctcggcatg ctcggcgaag
      721 agggggtgcg actgagcccc gtaatcgcga gagacatgct cgaggatgcg aagcgccgcc
      781 tccgcgaacg gacgtcccgc gagcgctcgc tcctgcgatt tccgcatctt caccgcggag
      841 accgcctgca tggccttcgt gaccttgctc gtgcgttcgg tcgcctttat cttgtttttg
      901 atctgcttga gcgccattgt cgattatgcg tagaggttcg ggtgggtctt cttgaactcg
      961 tcgactatcg cctgcagctt cgccgtcgtt tcgtccgaga gcgcgcgctc ctttcggata
     1021 gtctcgagaa tgtcgggcgt atcgcgctcg agctgttcga ggaacgtcct ctctatctca
     1081 cgtaccttct ctaccgacgc gccgtcgagg atgccgccgt tcgccgcgaa gatggagacg
     1141 acctcctcct caaacggcat cgtcgcgtgt tggtcttgct tcaagagctc cgtgtacttt
     1201 cggccgctct cgatctgctt cttcgtgttc tcgtcgagat cactcgcaaa ctgcatgaac
     1261 gcttcgagct ctcgaaactg cgcgagctcg agacggatct tgcccgagac cttcttcatc
     1321 gccttcgtct gtgcggccga gccgacgcgc gagacggaga taccgacgtt caatgccggt
     1381 cggattccct tgttgaagag atcggtctcg aggaagatct ggccatccgt gatggagatg
     1441 acgttcgtcg ggatgtacgc cgaaacgtcg ccctcttgcg tctcgatgat ggggagtgcc
     1501 gtgagcgacc cgccaccgag cgcgtcggag agtttcgccg cgcgctcgag gagacgagag
     1561 tggagataga agatgtcacc cgggtacgcc tcgcgtcccg gcggacggcg aaggaggagc
     1621 gacatctgcc gatacgcgac cgcgtgcttc gagaggtcgt cgtacacgat gagcgcgtcc
     1681 tttccgtcct ccatgaagaa ctcgccgatc gcagcgccgg cgaacggtgc gaggaattgg
     1741 agcggagatg gatccgccgc ggacgtggcg acgacgaccg tgtactccaa ggcaccgttc
     1801 tcctcgagct cgcggacgat gcgcgccgtc ttcgactctt tctgtccgac cgcgacgtag
     1861 atgcagatcg gccggttctc cttcggctcg tacttttggt tgatgatcgt gtcgatggcg
     1921 acggtcgtct ttcccgtacc gcggtcaccg atgatgagct ctcgctgtcc gcgaccgatg
     1981 gggatcatcg agtcgatagc cttgatgccc gtatggatgg gctcatcgac gccggcgcgc
     2041 tccatgacgc cgtacgcgac gcgctcgata ggcatgtcgc gagtcgcctc gtacgagccc
     2101 ttaccgtcga tgacctcacc gagcggactc acgacgcggc cgacgatgcc cgcaccgacg
     2161 ggaatactaa ggagtttgcc ggttcggcgc acggtcattc cctcggtgac gcgcgatgcg
     2221 tctccgagaa tgacgacctt caccgattcc tcctcgagat tgaggacgag accgaagagc
     2281 gaaccggtct cttccatcgc gtccttgagc gccgtaccct cggcatcctc gaagagcacc
     2341 atctccatca tgcgagcctc cttgacgccc tcgatctcgg cgacgccgtc gcccacggcg
     2401 acgacgatac ctatctccag agcctctcgc tcgggtgcga tggcctccag ttctttcttg
     2461 atgcgttcga tgatggtgct catatatatc gtggttatcg tgccgttaaa cgttggtacg
     2521 cgtgaaggag cttcgtctta aaactacggt cgatgcgctt tcccgacgcc tcggcgacga
     2581 agccgccgat gagattgtcg ttcacctcga ccgcgggctt ttcgctcgac ccgagacgcg
     2641 cgaaggcatc ggctatctcc cgctcgtagc gagcaacgtc gcgctcgcga gcaacgctaa
     2701 ggaccggtct ggacgtccgc tctcgacgtc gaaattcctc cgcgagtccg cgaaggatgc
     2761 gtggatacat cggagcgagc ccgcgaccgt cgaggtacgt gcggagagcg gagagcgtct
     2821 tgtcgacgtt tccctctcgc tcgatgacgt cgtatgctgc tttaacgtag tcgcggacgt
     2881 acatggtgtt cgttacttcg agagctcatc gcgaagcacg cgctcggctc cgagtatcgc
     2941 gagcttcgcg atctccgctt cgctctcctt ctgagcctgt gccttcagtt ccgctgcggc
     3001 gagcttcgcg ctctcgagct cgcgcgcgat gcgctcctgt gcttcattaa ggagctcttt
     3061 ccccttctct tcggcgtgct ccgtgccgcg cttcacgata tcggacgcct cccggtgcgc
     3121 gtcctcgagt acgcggtgct tctcgacgtc ggcgttcttg cgcgcctccg ccgcatcctc
     3181 ggcgtccttg acgccccgcg ctattttcga tgcgcgatcc gagagcatct taagaacggg
     3241 cgtatagagg aaataccaaa ggatgccgac gaggacgccg aagttgaaga cttgaatgac
     3301 gatgagtcgc cagtcgatgc cgaatgcctt gatgagttcc tgcatacgcc agggcggtta
     3361 gccgaatcga atgataaagc cgacgacgat cgcgaatatc gcgagcgcct cggtgaaggc
     3421 gataccgatg aacatgttcg tctggatcct gcccgagacc tccggattgc gaccgatcgc
     3481 ctcaagcgcc ttcgatacga ggataccgat accgatgccg gggccgatca tgccgagacc
     3541 gacggcgagc gccataccga gtaccttgat tgcttcgatg tccatactat atgtgtgctg
     3601 ttatctggtt actgctaatg gataactggg ggccgcgcac cgaagtgaga ggctcccagc
     3661 tacgcactag tgatgctcgg gttccgctat cgcgagcttg atgaagaaga gcgtcaagac
     3721 cgcgaatatg aacgcctgta tgaacccgac gaagagctcg aaggcgagta tcggaacagg
     3781 aagtataaag ggtacgaaga aaatcgcaat caggatgagc gtctttcccg cgaagatgtt
     3841 cccgaagaga cggaacgaga aggagacgag gcgcgcgagt tcgctgaaga gctcgatgat
     3901 gccgatgacg aacgcgaggg gcgacgcgaa gttgatgaac ttagaaccgt acttccagac
     3961 gccgagaaag gcgatgccgg tcacttcgac cgtgaagaag gctatgagcg cgagcgcgat
     4021 ggggatattg aggtcggtgt tcgccgagtg gaggaacggg acgaattcgc ttagtccgtc
     4081 ctcgatcttc cacatgccga gcgcgtcgac gcccgggagg agcgcgatga cgttgagcgc
     4141 gaagatgaag acgaaaatgc tcatgatgag cggaaagaac cgcttcgcga gttccctatc
     4201 ctcgagcgcg tcggcgacat aatcgaacgc tccaccgacg aagagttcga cggcgacctg
     4261 ggtcttcgag gggaccatcg cgaggcgcct tttcgcgacg atggcgaaga cgaggagaag
     4321 tgcgatcatc acccatgtgg tgagcaaggt cgcggtcaat ggaataccga agaaatggcc
     4381 gagtacgggg gcagaaaggt gaattgcgat accctccgat gcggcatgag cggtgttcat
     4441 aatttcgcgc gcatgataac agacaatagg tataaagaaa agccgaattc ctgcgtactt
     4501 tcaagcgata cgagccgctc aaggcctcat tcgtaccaca ggggtcatga gcgttcggtg
     4561 aaggcgatag acgggcattt cacggcggaa cgattgacca aacgccatat catgcgtatg
     4621 atatccgggt tccgttttgg gcccgtagct catttggcta gagcgcctgt tttgcactca
     4681 ggaggtgggg ggttcgaatc ctctcgggtc cactgatacg gagaaaccgg cttcatgccg
     4741 gttttttcgt atcaggctgg gtgtgggagg attcgaagac cttgagcagt cgctgcgcga
     4801 ccagattttc ctttagaaaa tcgaaatact cttgtggtga aaggtatacc gattctgtaa
     4861 gaatcgaatc ctctcgggtc cacacacgat ataaaacctg ccctgcggca ggttttatcg
     4921 tgtgtggaag ccggagcgtt gtttcggcta ttcctaatgt cgaaaccgcg aggcaggaca
     4981 ggaagcactt atgagcggag cgaattagtg acttgcgtca cagcaacccc actagaacat
     5041 cgcggttcgt atcttgttcc gtatggcatc gagccagtct cgtcgatacc gatacatgag
     5101 gaggatggaa ccggatatga cgaggagcgc tcccgcgagg gcgagctcgc gcttcaagtt
     5161 ccgtatgtac gcgacctccg ggttctcggg gtggtcggac gcggcctctg cgatgttcgg
     5221 caggaacgcg tcgatgatgc tcgcggcatc cgtcgtcgtc gcgaccgacg gctccgtcgt
     5281 cgagggcgtc gtcatcggtg ccggtacgag cggaaccgcc ggtatgtgcg ccggactcga
     5341 ccacacgagt atcgaaccgt tcattccgta cgttcgcgtc atgccggcgg cgtcgagcac
     5401 ggtctcgaga cgatagacac cgactggcgc atcgacatcg aacccgatgt catatccgat
     5461 ccgaacggtc tcgtgcgccg ctacggtacc gaggtcccat acctcctcgt ggagcacggc
     5521 gccctcggga tcacggagga cgtcatggag gaccacaccg tccacggctt catccccgtc
     5581 gttcgtgagg gtgagtcgct cggtcgtcgt cgccgccacg tcgaggcgta cgctcgtcgt
     5641 cgcgctctct cgctccacga ccaatgcgcg cggaaccgtg acgcttccgc ccgcgagcgt
     5701 ggtcggctcc ggcttcttcc cgtgcgagtt cgagccatgg tgatgcacgg cggtcgagac
     5761 agtcacgatg tcgctattgt cgaccatgtt gttgtccgtc tcatgctcca tgacgtcgta
     5821 cgtcgtatcg atgtctgtcc cttctcctgc gtttttcacc gtacccgagt acgtgagctc
     5881 gacggcaccg cccgcgggga tatcaccgag atgccagacg gcatctcgcc catcaagcga
     5941 caatggtacc gatgcgttcg agagcgcgag gtgatcggcg tcgtacgtcg ctcgaagggt
     6001 cacatccgtc gccgtagaat cgccgttatt gatgaccgtc accttatacg ccgcggtgtc
     6061 accgtttccg aaacccgtag gaacggtcgc ctggccaccg acccagagat cgggacgtcc
     6121 aaacgcgatg ttcccgttga agtctccgag aatgttcacg atcgcgagta tccagtttcg
     6181 accgacgacc gtcgcgttcg cgacgttcat gatgttcgcg ccggcatacg cgttacccgt
     6241 cgagatgagc gccgtgtccg cgttatgaat ctcgttctcg ccggtgagcg cgacgacgtg
     6301 gacgcggttc ttgatggatg cggcgttcgt cgtcgtcgca tagagcgagc ctccgcctcc
     6361 cgtgccgcct tcggtatcga agagatagat cccgccatcc ccattatcca tccaagcgag
     6421 cgtatccgga gcgccgaaca cctgaccggc ccatgacccg ttcacgcgaa aaatgatgga
     6481 gacgctcttc ccacccacga gcgacgtgtt gagttgattg aagacgttca cgcccgatgc
     6541 gctcgcaccg ctcgatatcg tcgaactcga cgcgtcggcc atattcgcgc cggtcgcggt
     6601 gtcgagggag atatcattgt cgatcgagcc ctcgttcgtt atggaaagcg cggctggtgt
     6661 ggtcgattgg gaaagttcac cgaagaactt ctcgaggctc gggaacacga tgtcgccgtt
     6721 cacgtcctga aacgcgttca tcgtgacgag gaggtagttc gaatcgatga cgttcgtgtt
     6781 cgccacattg acgagattga cgcccgcgta cgcgtctccg ctcgcgatcg cggcgttttc
     6841 cgcaccgtct atggtgttcc cgccggtttc cgcggtgacg tagagggagt tatcaagaat
     6901 cgcgccgttc tcgatgttcg tgtggacctc attatcggcg cagcccgaga gggagcacgt
     6961 cgatgagctc gcggcgaggt agtcgcgcag gtcgagaccg cccgtcagcg tatcgaagaa
     7021 attcttgaag agaaccacgc ccttcgagtt cacgaagttc gagttcacga gattgaggac
     7081 gttcgcgagc gcgaccgcgg tacccgtttt gatgacggtc gtcgtccccg ctcctccatt
     7141 gtcggtatcg acggtcgtcg acccgaggcc gttccattcg gtcgacgtcg ccgacgacgt
     7201 accggtcgag ctccatgtcg tggaactcgc gctcgtcgtc gcggtgagat gggcgaccgg
     7261 tgtcgtcgag ctcgaaagcg cgctcgtcga ggttccgctc tccatcgcgg ccgcatccgc
     7321 actgactgtt tcggtcgagg tcgcgtcacc cgagggcgcg ctcgacgtcg cgacagtgac
     7381 tgcgtcgtca gacggttccg ggtccgagga gacgacggcc tccgtcagag gcacggtatc
     7441 gttcgccagg cccgcctcga ccgggggcgt cgtcgtcccg tcatcggccc gtaccgtagc
     7501 gacgggaagt agcgcgagaa ggaggctcat gatgacgatt gggctcgcga tgcggagcgt
     7561 ggtggatgcg ttcatggtgt acagtggtta ttcgtaatga tcacctgttt tcatataaat
     7621 tctcgtcctg tgaggagaac ttatctgaaa acaggtggtg cctgttttca gatgtcacta
     7681 gtttcgagtg atccgggtaa gggatcgacc agagaccgtc acgaccttcg cgagcgaatc
     7741 ggatacgccg gtcacgaccg cgccgccctg gccgccgtta ccgccgttac cgccgcttcc
     7801 gcccgttgcc gagctgccgt caccgccgcc gaccgcgttc gcaccattgt cgccgatggc
     7861 gccgatagcg cccttcgagc gattgaaacc ggtgtacgcg tcgacgccga ccgagttctg
     7921 gaccgatgcg tcgttttggt tcgcgacgac gacgtcgtta tgcgcgacca cgtacgtccg
     7981 cgtgtaatcg atcgacttat tgtcgtgctg ttccgccgcc atttcgtcgg aagagttcga
     8041 ctcgcttgag acctgccaag cgctctcgtt gtcctgctga tagttcgacg taccggagtc
     8101 cgcagactgc gacgaatcgc tcgccgatgc ctggctccat gagccgtcgg tgtcgctcga
     8161 gtcctcggac gcagacgccg agttcgtgct ttggtacgtt tcgtgtgaat cagagccgtt
     8221 cgcgtagttc tgctcgttcg agctctcgtt gtacgacgag ttcgcagcct gtgactccga
     8281 accggagctt ccgaccgacg aacgcgcgtg gagcgtcaca tcgtactgat tcacgtcgct
     8341 acggttatcc tcgatcgtaa cctcggtcga gttgacgtcg ttctcgacgt cggccgttcc
     8401 gatagcgtcg ccggtcgtga tggaaccgcc gttgccgcca ttccctccgt taccgccgtt
     8461 gccgccgcga accgcgcttc cgtcgccgcc gcccaccgcg ctaccgccca tcgaaccgcg
     8521 agcgccttcg gcgcccttcg agacgttgcc gccggtgttc gacgcgacgg atacgttatt
     8581 cgatatcgac gccgaattcg tcgtcgaaac ggtgatcgtg tccgcgaacg cgaccggtgc
     8641 cgcgaggagg aggctcatcg tgctgataat ggatgccgct ttgtgtaaga tcatagactt
     8701 atgcttatgg tattaatgcg ctagtaatgg tctcccgacg ataccgaccg cggttcgcaa
     8761 tatgcgaacc gggcgctcgt acgttggacg ctcattgtaa tgaaagtggc atgataaaaa
     8821 gatgaaattt ttcggtcgat tttcatgaag atgaagtcga cggcgcgtac accccgcatg
     8881 tacggggagt tcatgagaaa aaacgctccg ttcatgaagc gttctcccat ttcattcatc
     8941 gctcactcaa aattcactgg tgcaatacgc aaagcgcggt cctcacggac cgcgctttat
     9001 cgcctactgt gcaatgtcgg ggtagccgga atcggaccga cgctacatcc accccatgga
     9061 cgcgtactac cactatacga taccccgacg ctggacggta agctcagccc taggattata
     9121 cagaaattcg cgactttcgc tacggcgcta tcgaatcgat gcgctccgct aactcgaagt
     9181 cgagcggcgt gagacccttc gcgtcatgcg tggagagacg gaccttgacc ttgccgtacg
     9241 agagttcgac gtcggggtgg tgtccgagta tctccgcatg caccgctatt ttcgcgacga
     9301 acgcgaggcc tgcgacgaac gatgggaacg agtacgtctt cgcgatctgg gtcgttgccg
     9361 cgtttaccgt ccagtcggga taattcttca gtcgtttctt gacctcgctc gccttcatcg
     9421 cctgtttcga acgaactgcc atatcggtat tatgacacac gaacgcggca cgcatcgtcg
     9481 atgaggtgag caaggaattc tccgtacgag cctccgaccg cctcgatcgc cttcggaagg
     9541 agcgactcgg gtgttaaccc gggaagcgtg ttcacctcga ggaaatagac gccatcgggt
     9601 gcgacgatga agtccgatcg cgagtactgg gagagcccga gctcgcggtg gacgagcttc
     9661 gcgaggcgct cgagttcgtg tttcgtcctc atatcgaagg tcgatggaca gagttctcgc
     9721 gtctcacccg agtacttcac ggtcgcgtcg aagtactcgg cgtgaggcgg cggcacgatc
     9781 tcgatggccg ggaatgcgta gaggtcgtgg ccgcggtagc gctccgcgac gccgcacgtc
     9841 gcctctctac ccttgatgcg ttgttccacc atgacctctt cgaactcgtc gagcgcttcg
     9901 cgaagtgcct ggtgaaggag gagcgggttc gtgaccatcc gcgtgccgac cgacgagccc
     9961 gaggcgaccg gcttcacgac gtactgcggg ccgaaaagcc gcatgatgcg ctcggcgacg
    10021 cgaccgatat cgtctcgcga ctcgcgcgtt acgtgaacgt gcggcgcgaa tcgaacgccc
    10081 gcgtcccgaa ggtggcgctt cgtgaggatc ttgttcatcg cgatgttcga ggcgtagact
    10141 ttgctccccg tgtacggaac ggcataccgc tcgaggagac gctggaacgt gccgtcctcg
    10201 ccgaacgttc catggagcgc gttgaacacg acgtcgaccg agtagagaat ctgctccggc
    10261 aaacggacgc gaccgtcgac gagccactcg cccgagcgaa gtatgatgac gtcgatcggc
    10321 gcgaagcgct cggggtcgag attcgcgatg accgcggcac cggtccgcat cgaaacctcg
    10381 tactcgtcgc tcatgccgcc gcgcactacc gctacgcgta ccctatgcat acgcatagtg
    10441 taccacggtg ccatttacgt cagttctggc gcatactgaa aaaccaccct cccgggtggt
    10501 tttcgttagg ccttcgcctt cttgatggcc gccatgaggc gggacttctt ccgcgaggcg
    10561 gtgttcttct tgatgacgcc gcgcttcgcc gccttatcga gtgcctcgta ggcgcgcgag
//
LOCUS       DASTSP010000015         4028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1149952, whole genome shotgun sequence.
ACCESSION   DASTSP010000015 DASTSP010000000
VERSION     DASTSP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149952"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFS75_00680"
     CDS             <1..274
                     /locus_tag="VFS75_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677216.1"
                     /translation="DIPPGLLRAPGIEKKVELRVREGCDNRGPGKSLLCEHLTHPEDE
                     DATSTDDGDDDSVSGRDHEEDDTASSTDDEGDDDSASSTPDGSNDD"
     gene            340..855
                     /locus_tag="VFS75_00685"
     CDS             340..855
                     /locus_tag="VFS75_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677217.1"
                     /translation="MTYSIHRAPIALIILLVTFFVLFVLPTRADAYVKTGESTIKADG
                     SIIFLMDYVFGHEKHEIGMPVIARDTDGALPSMLSYQIFDETGARVAGRAIGIVLSSA
                     RVDGTGEYVVPKGHSRKFTLMVIFTPEKNNAVSKYRLQVTSLPFYFDKTQLLRLNPSE
                     LTHYTTALVTP"
     gene            complement(845..2092)
                     /locus_tag="VFS75_00690"
     CDS             complement(845..2092)
                     /locus_tag="VFS75_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HEU4677218.1"
                     /translation="MSTSREYDVVVVGGGPAGMMAAGRAGERGLRVLLIEKNEVLGKK
                     LSITGGGRCNVTNAEDDIRTLLRHYGDAEQFLYSPFSRFGVRDTFAFIEAHGVPLVVE
                     ERKRAFPKTHKAPDVTRAMERYVADGKVTVLLKTAVQGFETTDGRIAGVVTDRGTFVG
                     RAYVIASGGKSHAETGSSGESISWLRAVGHTAHEPNPNLVPLVVEDAWVKRLAGTVLR
                     GARIAFNGATDKLVKDGDILMTHFGLSGPTILNAAHEVKELLSRGAVNAEIDLFPHED
                     EGALRTRLNELLQVHSNKNLQNALRAWFPNALAETLIGSAENARLKAHSVGRELRGEL
                     TLRMKRMPITVTGTMGYDWAIVSDGGVDLSEIDTRTMRSKLHENLYLVGDVLNINRPS
                     GGFSLQLCWTTGWLAGESACATE"
     gene            2146..2850
                     /locus_tag="VFS75_00695"
     CDS             2146..2850
                     /locus_tag="VFS75_00695"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU4677219.1"
                     /translation="MRLNKFLAHAGYATRRSADALIEAGRVFVNGKRAVVGQKVTETD
                     KVELKDYDATNFRYLLYYKPRGVITHSPEGDEVDIETRIRRDCGVTGVFPVGRLDKDS
                     EGLIILTNDGRITGPLLSPEHGHERVYEVDTDKRVTQTFLNALAKGVDIEGYRTKPAK
                     ADFLPGNEHGLRLTLTEGKKHQVRRMCAALGYQVRRLRRVRIDAYELKRLKPGAFYEL
                     SKKEAAHFRRRLGLPA"
     gene            complement(2903..3109)
                     /locus_tag="VFS75_00700"
     CDS             complement(2903..3109)
                     /locus_tag="VFS75_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU4677220.1"
                     /translation="MKGKIARLTDRGFGFITPEEGGNGKDLFFHARSLADGLLYDSLK
                     EGDIVTYDVEDGEKGPAAVNVKLA"
     gene            3345..3728
                     /locus_tag="VFS75_00705"
     CDS             3345..3728
                     /locus_tag="VFS75_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677221.1"
                     /translation="MTFLWKAAIMAVLAVVFLSPSRAVFAAETYDVAPIGALATYRFD
                     NENWFTLPHPKVASFTVEDDGNFSGMTEDGIPFTQRNVPNTLGVRIQRFEIQDHYHFI
                     VEGEEAYSADELAVKVERILTERGQ"
     gene            complement(3763..>4028)
                     /locus_tag="VFS75_00710"
     CDS             complement(3763..>4028)
                     /locus_tag="VFS75_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008308428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU4677222.1"
                     /translation="QWGKAQNVSKVKLIPDGSGDFTRGLGMLVKKNDVGFGDRSWRYS
                     MYVEDGEIKKAFIEPGFRDDCPTDPFEVSDVDTMLTYLKGLKS"
BASE COUNT          816 a         1274 c         1128 g          810 t
ORIGIN      
        1 ggacattccg ccgggactcc tccgcgcgcc gggtatcgag aagaaggtcg aactccgcgt
       61 tcgcgagggt tgcgacaacc gcggcccagg aaagagtctc ctctgcgagc acctcacgca
      121 ccccgaggac gaggacgcga cgtcgaccga tgatggcgac gacgacagtg tgagcggccg
      181 cgaccacgag gaggatgata ccgcctcttc gacggacgac gagggggacg acgactcagc
      241 ctcttcgacg ccggacggaa gcaacgacga ctagccctcc acagaaacaa aatggccgcc
      301 ttcaggcggc ctttttgttt ctgcggtata cttcactcta tgacatacag tatccatcgc
      361 gcgcctatcg cgcttatcat cttgctcgtg acatttttcg tgctctttgt gcttccgact
      421 cgagcggacg catacgtgaa gaccggcgaa tccaccatca aggcggacgg ctcaatcatc
      481 ttcctcatgg actatgtctt tggccacgag aagcacgaga tcggcatgcc cgtcatcgcg
      541 agagacaccg acggagcgct tccctcgatg ctctcgtacc agatattcga tgaaacgggc
      601 gcgcgcgtcg ccggccgcgc gatcggtatc gtccttagtt cggcccgggt cgacgggacg
      661 ggtgagtacg tcgtcccgaa gggtcactcg cggaaattca ccctcatggt catcttcacg
      721 ccggagaaga acaacgccgt atcgaaatac cggctccagg tgacgtcgct ccccttctac
      781 ttcgacaaga cacagctgtt gagactgaac ccctccgagc ttacgcacta tacgaccgcc
      841 ctcgtcactc cgtagcgcac gcgctctcgc cggcgagcca cccggtcgtc cagcagagct
      901 ggagcgagaa accgcccgat ggacggttta tgttgagcac gtcgccgacg aggtacagat
      961 tctcgtgaag tttcgagcgc atcgttctcg tatctatctc ggagagatcg acgccgccat
     1021 ccgatactat cgcccaatcg tatcccatcg tgccggtcac cgtaatgggc atgcgcttca
     1081 tgcgtagggt gagttcgccg cgaagttctc gaccgacgct gtgcgccttc agccgcgcgt
     1141 tttcagccga accgatgagc gtctcggcga gcgcgttcgg aaaccaggcg cgaagcgcgt
     1201 tctgaagatt cttgttcgag tggacctgaa gaagttcgtt gagtctcgtg cgtagcgcgc
     1261 cctcgtcctc gtgcggaaaa aggtctatct ccgcgttcac cgctcccctg cttaagagct
     1321 ctttcacctc atgcgcggcg ttaaggatgg tcgggcccga cagaccgaaa tgggtcatca
     1381 aaatgtcacc atctttcacg agtttgtccg tcgcgccatt gaaggcgatc ctcgcgccgc
     1441 gaagcaccgt tcccgcgaga cgttttaccc acgcatcctc tacgacgagt ggaacgaggt
     1501 tcggattcgg ctcatgagcc gtgtgaccaa cggcgcgaag ccacgagatg ctctcgccgc
     1561 tcgagcccgt ctcggcgtgc gacttaccgc cgcttgcgat gacgtacgcg cgccccacga
     1621 acgtaccgcg gtccgtcacg acgccggcaa tgcgtccgtc ggtcgtttcg aacccctgca
     1681 ccgcggtctt aaggagtacc gtgaccttac catccgccac gtaccgctcc atggcgcgcg
     1741 tgacgtcggg agccttatgc gtcttcggga aggcgcgctt tcgctcttcg acgacgagcg
     1801 gcacgccgtg cgcctcgatg aacgcgaagg tgtcgcggac gccgaagcgt gagaagggcg
     1861 agtacaggaa ttgttccgca tcgccgtagt gccgaaggag ggttcggata tcgtcttcgg
     1921 cgttcgtgac attacatctt ccgccgcccg ttatggagag tttcttcccg agcacctcgt
     1981 tcttctcgat gaggagtacg cgaaggccgc gctctccggc gcggccggcc gccatcattc
     2041 ccgccggccc gccgcccacg acgacgacat catactccct gctcgttgac atagtgcgaa
     2101 aatactagca taatcaagcc tcaaatcaaa tctatggact ttcccatgcg actcaacaaa
     2161 ttcctcgcgc acgcagggta cgcgacgcgc cgaagcgcgg acgctctcat cgaggccggg
     2221 cgcgtcttcg tgaacgggaa acgagcggtc gtcggacaga aggtcacgga gaccgacaag
     2281 gtcgaactca aggattacga cgcgacgaac ttccgctacc tcctctacta caagccgcgc
     2341 ggcgtcatca cgcactcgcc cgaaggcgac gaggtcgaca tcgagacgcg catcaggcgc
     2401 gattgcggcg tgacgggcgt ctttccggtc ggccgcctcg ataaggactc cgagggtctc
     2461 atcatcctca cgaacgatgg gcgcatcacc gggccgctcc tcagccccga gcacggccac
     2521 gagcgcgtgt acgaggtcga caccgacaag cgcgtgacgc agacgttcct caatgcgctc
     2581 gcgaagggcg tcgacatcga gggataccgc acgaagcccg cgaaggcgga cttccttccc
     2641 gggaacgagc acggcctccg gctcacgctt accgagggaa agaaacatca agtacgccgc
     2701 atgtgcgcgg cactcggcta ccaggtgcgc cgcctccgtc gcgttcgcat cgacgcgtac
     2761 gaactgaagc gcctgaaacc cggcgccttc tacgaactct cgaagaagga ggcggcgcac
     2821 ttccgaaggc gcctcggcct ccccgcctaa tacggaaaaa gccgcggacg agtccgcggc
     2881 tttttctata actggactgg ctttatgcga gcttgacgtt cacagcagca ggtcccttct
     2941 caccatcctc cacgtcgtac gtcacgatgt caccctcctt gagtgaatcg tagagcagtc
     3001 cgtccgcgag cgatcgcgca tggaagaaaa ggtcctttcc attgcctccc tcctcgggag
     3061 taatgaaacc gaatcctcgg tcggtgagac gagcgatttt acccttcatt gatctatgat
     3121 cattcttatt gaataaacaa aaccgatgtg cttctgatcc gactagctca ggccctatgt
     3181 tttgtatgaa aatactacca tgcttattcc gccgtgtcga tagcatgtgg atgaagatta
     3241 cgtgtcgtat gtattgacgg acatcgatat gagcgtatag tacggctaac gtagcgctcc
     3301 ttggggctgt gccccgccct ttttaccgaa ataagccgta ttatatgact ttcctctgga
     3361 aagcggccat tatggccgtt cttgccgtcg tctttctgtc gccctcccgg gcggttttcg
     3421 cagcagagac gtacgatgtt gccccgatcg gcgcgctcgc gacgtaccgc ttcgataacg
     3481 agaactggtt cacgctcccc caccccaagg tcgcgagctt tacggtcgaa gacgacggta
     3541 atttctcggg tatgaccgag gacggcattc ccttcactca gcggaatgta ccgaacacgc
     3601 tcggcgttcg catacagcgt ttcgagatac aggaccacta ccacttcatc gtcgagggcg
     3661 aggaagcgta ctcggcagac gaactcgccg tgaaagtgga gcggatactc acggagcgcg
     3721 gacagtagac gaaaaacacc ggccgtgccg gtgtttttcg ttttaggact tcagtccctt
     3781 cagatacgta agcatcgtgt cgacgtccga gacctcgaac gggtccgtcg ggcaatcgtc
     3841 cctgaatccc ggttcgatga acgccttctt gatctctccg tcctcgacgt acatgctgta
     3901 gcgccacgaa cggtcgccga atccgacgtc gttcttcttc acgagcatgc cgaggccgcg
     3961 cgtgaagtct cccgagccgt ccggaatgag cttcaccttg gacacgttct gcgcctttcc
     4021 ccactgga
//
LOCUS       DASTSP010000016         5145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_37604, whole genome shotgun sequence.
ACCESSION   DASTSP010000016 DASTSP010000000
VERSION     DASTSP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37604"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3216)
                     /gene="dnaE"
                     /locus_tag="VFS75_00715"
     CDS             complement(<1..3216)
                     /gene="dnaE"
                     /locus_tag="VFS75_00715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4677223.1"
                     /translation="MDGNDRKSDFIHLHVHSHYSLLNALPTPKELAARARDEGMHALA
                     LTDNGGLYGAVNFYKACAKEGIKPILGIDAYLAPRSRLEKDAGLDKPRGRVVLLAKNN
                     DGYTNLIKMVTESYVNGFYYRPRIDHTLIEELHDNLLCIIPSFSGEPVLDLKDGRDEE
                     AKASLDWYRSLFGEDCYIELTHHPEIEGHEALQRRILELAEATKTPLVAAHDVYYLDQ
                     KDHLARETMVKIQSGGVVETEDAEGLLAPNFSFISEARAKELFTGPAQREALANTVRI
                     ADRCTVEMTPGETWYFPDYTIQSGKSPDEELQDMAYDGVAWRGLSLLDKEVKARLEYE
                     LSVIKMKGYAKYFLVVGDLIREARERKILTTIRGSVAGSLTTYVLGITNVNPLDYKLP
                     FERFLNPERPSAPDIDMDYADNRRDEMIAYAREKYGEANVAQIGTFGTMMARAAVRDV
                     ARVLGHPYATGDRIAKLIPMGSQGFPMTIAKALEIEPDLRELYAKDDDTKQVIDLARQ
                     IEGRVRHVGVHAAGVVIAPRPLTEFVPVQPDPKTGKLITQYDMHSVGEDGVGLLKFDF
                     LGIKNLSILAEAVRRVRVIHGIEVDIEKIPLDDGKTFEMLARGETMGLFQLNGSGMTA
                     FLKQLRPTDIHDINAMVALYRPGPMESIPQYIERKHNSSLVTYLDPRLSKILDRSYGV
                     ITYQDDVMMISIELAGYSWLDADKLRKAMGKKIPAVMEAEKEKLMKGFLEYGKLSPAI
                     AEKLWKLIEPFAAYGFNKAHAASYGRVAYQTAYMKANYPVEYMSAVLTADAGDTEKIA
                     EIINECTRMGIEVLPPDVNESFAPFSVVPAKEDVPAKIRFGLTTIKNFGEGIAEEIIE
                     ERKAHGAYASMQDFLSRIASRNLNKKSLEALIMSGCFDRFGERGMFMHNLETLLSYQR
                     ERSHGKESAQDSLFGGMEGSTIHDLALEPATPATSNEKLLWEKELLGVYVSGHPLDAY
                     REELAKRPKIADIKKETRNGIPVVTAGMVESVRELLTKKGDRMAFVQLSDGLASVELV
                     AFPEAYRAHHELFAAGQCIAIQGKLSMRNDEPTVVIDRAKRL"
     gene            3242..3901
                     /locus_tag="VFS75_00720"
     CDS             3242..3901
                     /locus_tag="VFS75_00720"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU4677224.1"
                     /translation="MKSENGLKMDVFSPRYIVGVDEAGRGPLAGPVAVGAVVVSHDFD
                     WALLPGVDDSKRVPAVERLRIYGEAKALKASGVLDYAVSLVAPSVIDRAGIVRAVARG
                     ISRSLSKLALDPTLVEVRLDGLLRAPRAYGFQRTIVGGDGLEKVIGLASIMAKVTRDR
                     FMERVARRYPHYGFDAHKGYGTPEHRKAILEHDLCPLHRRSYCTRFTVQNGLVEILDE
                     V"
     gene            3960..4139
                     /locus_tag="VFS75_00725"
     CDS             3960..4139
                     /locus_tag="VFS75_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677225.1"
                     /translation="MKEENGGGPLRQFGEANMLPAHKLEPDDTSHLSDRRRLRSLQSP
                     SSELRIGGVPLKESA"
     gene            4410..4685
                     /locus_tag="VFS75_00730"
     CDS             4410..4685
                     /locus_tag="VFS75_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677226.1"
                     /translation="MEHFRGVGRKISGVHDVKARPVNGHLIQIWLYGQMGESERDTIR
                     DAMLGTLKGRIKPFMHRLKWPGFRVKTASHPKGEVKEVCWCIERRPR"
     gene            4812..4988
                     /gene="rpmF"
                     /locus_tag="VFS75_00735"
                     /pseudo
     CDS             4812..4988
                     /gene="rpmF"
                     /locus_tag="VFS75_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
BASE COUNT          938 a         1520 c         1642 g         1045 t
ORIGIN      
        1 gagtcgcttc gctcggtcga tgacgacggt cggctcgtcg ttacgcatcg agagcttccc
       61 ctggatggcg atgcactgac ctgcggcgaa gagctcgtgg tgcgcacggt acgcttcggg
      121 gaacgcgacg agctcgaccg aggcgaggcc gtcggagagt tgtacgaacg ccatgcggtc
      181 gcccttcttc gtgaggagct cgcggacgct ctccaccatg cccgcggtca cgacggggat
      241 gccgtttcgc gtctccttct tgatgtcggc gatcttcggg cgcttcgcga gctcctcgcg
      301 gtacgcgtcg aggggatggc ccgagacgta gacgccaaga agttccttct cccagaggag
      361 cttctcgttc gacgtcgcgg gcgtcgcggg ttcgagcgcg aggtcgtgta tcgtcgaccc
      421 ctccatgccg ccgaagagcg agtcctgtgc ggattccttg ccgtgcgagc gctcgcgctg
      481 atacgaaagg agcgtctcga ggttgtgcat gaacatcccg cgctcgccga agcggtcgaa
      541 acagccggac atgatgaggg cctcgagcga tttcttgttg aggttcctcg atgcgatgcg
      601 cgagaggaag tcctgcatcg acgcgtacgc tccgtgcgcc tttcgctcct cgatgatctc
      661 ctccgcgatg ccttcgccga agttcttgat ggtcgtgagg ccgaagcgta tcttcgcggg
      721 gacatcctct ttcgcgggaa cgaccgagaa cggcgcgaag ctctcgttca cgtctggtgg
      781 gaggacctcg atgcccatgc gtgtgcactc gttgatgatc tcggcgatct tctcggtatc
      841 gccggcgtcc gcggtgagca cggcggacat gtactcgacg gggtagttcg ccttcatata
      901 cgcggtctgg tacgcgacgc gaccgtaact cgccgcatgt gccttgttga atccgtacgc
      961 ggcgaacggt tcaatgagct tccagagttt ctccgcgatc gcgggagaga gcttcccgta
     1021 ttcgagaaag cccttcatga gcttttcctt ctcggcctcc atgaccgcgg gaatcttctt
     1081 acccatcgcc tttcggagtt tgtccgcgtc gagccagctg tagccggcga gctcgatcga
     1141 tatcatcatg acgtcgtcct ggtacgtgat gacgccgtac gagcggtcga gtatcttcga
     1201 aaggcgcggg tcgaggtacg tgacgaggga actgttgtgc ttgcgctcga tgtactgcgg
     1261 gatggattcc atcgggcccg ggcggtagag cgcgaccatg gcgttgatat catgaatatc
     1321 ggtcggccgg agctgtttca agaacgccgt catgccgctg ccgtttagct ggaagagacc
     1381 catcgtctca ccgcgcgcga gcatttcgaa ggtcttccca tcgtcgagcg gtatcttctc
     1441 gatgtctacc tcgataccgt gaatgacgcg cacacggcga accgcttcgg cgagtatcga
     1501 gaggttcttg atgccgagaa agtcgaactt gaggagaccg acgccgtcct cgccgaccga
     1561 gtgcatgtcg tactgcgtga tgagcttacc cgtcttcggg tcgggctgga ccggcacgaa
     1621 ctcggtgaga gggcgaggcg cgatgacgac accggccgcg tggacgccga cgtggcgaac
     1681 gcgtccctcg atctgccgcg cgaggtctat gacctgcttc gtgtcgtcgt ccttcgcgta
     1741 gagctcgcgg aggtcgggct ctatttcgag ggctttcgcg atcgtcatcg ggaagccttg
     1801 gctccccatc gggatgagct tcgcgatcct atcgccggtc gcgtacgggt ggccgaggac
     1861 gcgcgcgaca tcgcggacgg cggcgcgtgc catcatcgtc ccgaacgtac cgatctgtgc
     1921 gacgttcgcc tcaccgtact tctcgcgcgc gtaggcgatc atctcgtcgc gacgattgtc
     1981 ggcgtagtcc atgtcgatgt caggcgcgga cggacgctcc gggttgagga atcgctcgaa
     2041 ggggagtttg tagtcgagcg gattcacgtt cgtgatgccg aggacgtacg tcgtgaggga
     2101 ccctgcgaca gaaccgcgga tcgtcgtgag tatcttccgc tcgcgcgcct cgcggatgag
     2161 gtcaccgacg acgaggaaat acttcgcgta tcccttcatc ttgatgaccg agagctcgta
     2221 ctcgaggcgc gctttgacct ccttgtcgag aagggagagc ccgcgccacg ccactccgtc
     2281 gtacgccatg tcctggagct cctcgtcggg cgacttaccg ctttgtatcg tatagtcggg
     2341 gaagtaccac gtctcgccgg gcgtcatctc gaccgtacag cggtcggcta tcctcaccgt
     2401 gttcgcgagg gcctctctct gtgccggccc ggtgaagagc tccttcgcac gcgcctcgga
     2461 gatgaacgag aagttcggcg cgaggagccc ctcggcgtcc tcggtctcga cgacgccacc
     2521 cgactgtatc ttcaccatgg tctcccgcgc gaggtgatcc ttctggtcga ggtagtacac
     2581 gtcgtgcgcg gcgacgaggg gcgtcttcgt cgcctccgcg agttcgagga tgcgtctctg
     2641 gagcgcctcg tgcccttcga tctcggggtg atgcgtgagt tcgatgtagc aatcctcgcc
     2701 gaagaggctc cggtaccaat cgagggaggc cttcgcttcc tcatcgcgtc cgtctttgag
     2761 atcgagaacg ggttcgccgc tgaaggacgg gatgatgcag agaaggttgt cgtggagctc
     2821 ctcgatgagc gtgtggtcga tgcgcggacg atagtagaaa ccgttcacgt acgactcggt
     2881 caccattttg atgaggttcg tgtagccgtc gttatttttc gcgaggagga ccacgcgtcc
     2941 gcgcggcttg tcgagccccg cgtccttttc gagtcgtgaa cgcggcgcga ggtacgcgtc
     3001 gatgccgagg atcggcttaa tgccttcctt cgcgcacgct ttatagaagt taacggcgcc
     3061 atagagaccg ccgttgtcgg tgagggcaag agcgtgcatg ccttcgtccc gagcccgcgc
     3121 cgcgagttcc ttcggggtcg ggagagcgtt tagaagcgag tagtgcgagt gaacgtggag
     3181 gtggatgaag tcgctctttc ggtcgttccc gtccatagtg acagtattgt atattagatg
     3241 tatgaaaagc gaaaacggcc tcaaaatgga cgtgttttcg ccacggtaca tcgtcggcgt
     3301 cgatgaagcc gggcgcggtc cattggctgg ccccgtcgcg gtcggtgcgg tcgtcgtctc
     3361 tcacgatttc gactgggcgc tcctcccggg tgtcgatgac tcgaaacggg tccctgccgt
     3421 ggagcggctc cgcatctatg gggaagcgaa ggccctcaag gcgtccggtg ttctcgatta
     3481 cgccgtctcc ctcgtcgctc cctccgtcat cgaccgcgct ggcatcgttc gagcggtcgc
     3541 gcgcggcatc tctcgctcgc tttcgaaact cgcgctcgat ccgacgctcg tcgaggtgcg
     3601 actcgatggg ctcctacgcg caccgcgcgc gtacggattc cagaggacca tcgtcggagg
     3661 cgacggtctt gagaaggtca tcggtctcgc gtccatcatg gcgaaggtca cacgcgatcg
     3721 cttcatggaa cgggtcgcac gccgctatcc gcactacggc ttcgacgccc acaaagggta
     3781 cgggacgccg gagcatcgga aggcgatcct cgagcacgac ctctgcccgc tccaccgacg
     3841 ctcgtactgt acgcgcttta cggtgcaaaa cgggcttgtc gagattctgg acgaggtata
     3901 gcatgaagat actccctgta ccgggcggca cgggagaaac cacaacggga gagtcaacga
     3961 tgaaggaaga aaacggagga ggacctctgc ggcagttcgg ggaagcgaac atgcttccgg
     4021 cccacaagct cgaaccggat gacacgtcgc acctgtcgga tcggcgtcgg ctccgttcgc
     4081 tccagagtcc ctcgagcgaa ctgcggatcg gcggcgttcc cctcaaggaa tcggcctaac
     4141 gcggcaacgc ggccccagac cgacggaaac gcatttccgc cggtcctttt tattcgacga
     4201 aatcggttgt gcgtggtata gtatcgcgtc gttcgtaagg aggcgaacga agacaatggg
     4261 gaccgccatg tggcggacgt tcaatcaaga agaagggaaa ggagacaccg ttgcgtgtca
     4321 tcgaaaaaga agtgaaggtg gcgaggggaa catccctcga ctactcgaaa agttctctcc
     4381 gggtcgcatc ggacgtaagc gtgctcgtca tggaacactt tcgcggggtc gggagaaaga
     4441 tctccggcgt ccatgacgtg aaggcgcggc cggtcaatgg acacctcatc cagatctggc
     4501 tctacgggca gatgggagag tccgagcgcg ataccatccg cgacgcgatg ctcggcacgc
     4561 tgaagggtcg catcaagccc ttcatgcacc gcctcaagtg gccgggattc cgcgtcaaga
     4621 cggcatccca ccccaagggc gaggtgaagg aagtgtgctg gtgcatcgag cgccggccac
     4681 ggtagcacga atccagtttc ccacgaggaa ttccgcccca cggggcaagg ccgcttcgat
     4741 cacgagcggc ctttttattt cgcccatggt ttagttgctt tttcagcggc atttggtata
     4801 gtcttgccac tatggtcata cgaatgcgac atactcggtc ccacacgggg aaccgccgct
     4861 cacaccacgc gctcaaggcg gcgaaccttt cgaagtgttc gcactgtggc gaactccacc
     4921 gtccgcacca catgtgcctc tcgtgcggct actacaacgg ccgccaggtg atggacctca
     4981 aggcggagaa ggcgaagcgc gaccagcgca tcaaggcgaa ggaggagatg atcaagaacg
     5041 aggctgtctc gtcgagcgcg cccgccgacg agaaggagga gaagaagtag gcgcgaccta
     5101 aatccacaaa acgtcccccg aaagggggac gtttccgtag aaaat
//
LOCUS       DASTSP010000017        13908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_929406, whole genome shotgun sequence.
ACCESSION   DASTSP010000017 DASTSP010000000
VERSION     DASTSP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13908
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929406"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..631)
                     /locus_tag="VFS75_00740"
     CDS             complement(26..631)
                     /locus_tag="VFS75_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677227.1"
                     /translation="MNRTSFLAAILVGVALSSAAFATPTKLELRVICETPEVAKFRAD
                     RQGRTNQPSWAWNASHNVKKHVRGFKYAGPGLVLTVQIDGVRRDLNVDGKSFRVETPD
                     KTWDYRVTGEVAASGAPGVVRFGTIDMKSGAVTIELGDIIDPDPHAPLTVTIWPQNFE
                     GTGLTYDSGRKYYVADMRYRKDNGTMLPEIVAIPRRGICGR"
     gene            complement(700..1917)
                     /locus_tag="VFS75_00745"
     CDS             complement(700..1917)
                     /locus_tag="VFS75_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677228.1"
                     /translation="MFRSKRFWVAVLVLLAAAALAFWLLAAADVVCVARTKVVPDPRI
                     TENDAVFAEAVRDARTLAFSSLVRSLDPVTSYFTYEEHPNGTISNADNDIRQLLASRV
                     LAEAAAFDSSYETLHERNLSVIMENWYKEDGEQAYVYAYGKSKLGANAMLLRVLVASP
                     DFARYATEAKRLAAGILSLMHEDGSFEPWYKEPDYAYDRDYLLTFYSGEATLALLEYG
                     EKVKDVAVLTDARRAEDHYIGAYVDDMSAHYYPAYVPWHTLALSHLFNATKDMRYANA
                     IFALNDKLLELEDMEEFIGRFYDPETPEYGSPHASSDAVYTEGLATAYAVAKATGDAA
                     HARAYRGTLTLAFQNLVNLQYEAPWYRIFDDHTARRKLLGGIMTNACEAQVRIDSTAH
                     AVDAFDAILRAVS"
     gene            complement(1935..2600)
                     /locus_tag="VFS75_00750"
     CDS             complement(1935..2600)
                     /locus_tag="VFS75_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEU4677229.1"
                     /translation="MDHTEGRIEELYASKLEQRVVFFFVLVGIIAVTYGLLYLIDFIP
                     EKPGTNDAAKEALTKPATTTPAEPAPVAVDPYPVSITFDSLDKELTIQNPTSDDVSVL
                     DTALLSGVVRHPDSADFEHTGTIFLLGHSSYLPVVHNKNFQAFNGIQKLKWGDTIRLR
                     SKDTEYVYAVDRVYEAKASSAEVPIAHGVAKLTLATCNSFATKDDRFIVEASLVSSKP
                     LRS"
     gene            2742..3746
                     /locus_tag="VFS75_00755"
     CDS             2742..3746
                     /locus_tag="VFS75_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677230.1"
                     /translation="MKETHQYRILMIKTLAIIGFLVTLGLVVWLIVAGLRLIPGAFSS
                     LASIAESIKNYRPLPSLELTLEKTAVDSGESFELHWTDMGTEGTFHFSYGCTDGVGLG
                     VRGQDGVFVPIRCTDSLGLPADVHGLFLSVTSDAMRVADVPLTLSYENDAKHVTKEAK
                     ATVTVANANIGEKPATTTPVVTPSPKPATTTATSSKPVTPTPAPKPVTRVVYPPSDPN
                     GYTDLAVTTLGSGIIRNGAFSFTASYERDAKNAIRFDVKNVGTKTSDEWYFKTLLPSG
                     ETYTSPTQAPLKPYEHIEFTLGFDLDTNANLVTIVNSAFTDRDVNAKNDSAAWSVVVK
                     "
     gene            complement(3806..5887)
                     /locus_tag="VFS75_00760"
     CDS             complement(3806..5887)
                     /locus_tag="VFS75_00760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HEU4677231.1"
                     /translation="MAKKDTAKSTYSADSIQVLEGLEPVRKRPGMYIGTTGPDGLHHL
                     IWEVFDNCRDEAMGGFANRIEVALLPGNYVRVVDNGRGIPVDIHKQTKVSALETIMTT
                     LHAGGKFGGEGYKVSGGLHGVGVSVVNALSSHVIAEVHRDGAYYIQEYHIGKPKAKVK
                     KVGPSKLQGTIITFQADDTIFEEVKYEWRRIVTHLRQQAYLVKGLKISIIDAREMSEE
                     AQRKLDVEGKQYLTDEALEVPHEHFYFEGGLKSLVAFNNQYQKPVHDRIFYVEKQDVD
                     AKVLSVEVALQYVDDITARISAFANNIYNPEHGTHVTGFKTALTRTLNNYAKKNNFFS
                     VKEKDAGFTGDDVLEGITAVVSVKMPEIQFEGQTKAKLGSVEARSAVESVFGDAFATF
                     LEENPDDAKAIVNKVVIAVKARKAAKAAKDSVLRKGALEGMTLPGKLADCQSKSAEES
                     ELFIVEGDSAGGSAKMGRDRRTQAILPLRGKILNVERARLDRMLGSEQVKNLVVALGT
                     AIGDVFDIGKLRYHKIIIATDADVDGAHIRTLLLTLFYRYFKPIIDGGYLFIAQPPLY
                     KIQRGKEVLYAYSDEEKMTLLGEDAVVEDVESDGEPEAESEEAAEVEEKEEAAKSKKV
                     RIQRYKGLGEMNAEELWETTMNPENRVLKQVSILDAQAADKVFDILMGTDVSSRKSFI
                     QSNARMANLDV"
     gene            complement(6034..7959)
                     /locus_tag="VFS75_00765"
     CDS             complement(6034..7959)
                     /locus_tag="VFS75_00765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU4677232.1"
                     /translation="MKVLYDWLKDYTGDALPSLPELENLLTFHAFEIDGIEEIAGNEV
                     IDVKVLPDRGSDCLSHRGIAREIATLTGTPLQADPLTTPVSLTPETDSLTVSIEDPSR
                     CRRFSAARITGLTVKDSPEWLRARLAALGQRSINNVVDATNYVMLGLGQPLHAYDAEK
                     FPHEGGVWRFGVRMAREGEEVTILGGETYTASPEVQLIVDAKSDAPAGIAGIKGGQLA
                     EIDASTTAIILEAANFDPRTTRRAAQSLRLPTDASKRFENDLSPELAAYGLTAAVALI
                     LDIAGGTLEGYVDAYPTPRENPRVDVSLAHIEGLLGMSLGAEAIEDILRRLGFSYERK
                     GDAWAVIAPFERTDIVIPEDVIAEIGRVYGYEHVDAIMPPPAPLAEVNARHYYAERIR
                     ETLVGLGFSEIITSSFRKKDEIKLRNALASDKGYLRSDLRKNVKEALLRNAPYMDLLG
                     VSRIQLFEIGTVFCRTDDGADVTEHVSLALGVRTKAGGYAPADDARLREALTALESSL
                     GAPLTTDVHDGVAECDLTSVIANLPSPAAYEPFTPPADVRFRPFSEYPFVSRDIALWV
                     KETVAAADIEAVLREGAGDLLVRLSQFDEFKKDGRVSYAFRLVFQSKERTLTDEEVGV
                     IMERISGMLAERGYEVR"
     gene            complement(7961..8686)
                     /gene="pheS"
                     /locus_tag="VFS75_00770"
     CDS             complement(7961..8686)
                     /gene="pheS"
                     /locus_tag="VFS75_00770"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU4677233.1"
                     /translation="MNASERGHRHPISSMIAEINAIFAEIGFVFAEGPEAERERYNFD
                     RLNVPADHPSRDMQDTFWFSSKDVKEPTVLRTHTSPVQARYMETHTPPIRVIVPGKVF
                     RNEATDATHEAQFYQLEGLYVDKGVHLGHLKGTIEYFFEKFFKGDVEVRFRPSFFPFV
                     EPGVEVDMRITSGASKLKGRWIEVMGAGMVHPSVLEGSGIDPATYSGFAFGMGIDRLG
                     FMKYEFDDVRDLYSGDLRFVNQF"
     gene            complement(8689..9822)
                     /locus_tag="VFS75_00775"
     CDS             complement(8689..9822)
                     /locus_tag="VFS75_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677234.1"
                     /translation="MEQSEYVKPLPYNSRRNLFAFLVIVFLVVLPFLYLYATGYRFDF
                     AKPTNIVSTGGIYVAADRTGAQIYIDDELVRETRAFRQAFYAQNLDVGTHRVHVQKDG
                     HHTWVKELPVSEHLVTEAAAFNMPLIPDARLIAKWDTATGSPVVKSEIAYPHVVNAYV
                     ATTTKPTAASFKLDQEYTSLMAHFASTSTSTASRETTAEYLRGLLTGGGATTTDEATS
                     TKISSGVRLALEGDDLFATWVGPFEQMPYYYCAPTFPPYSTSTPAISSAVTTPAVRQD
                     VDPDGLVMHPVQAVPAGAACDPTVKMDRKGETLRAFDFYPGSTDLILEVLEDGIYVVE
                     IDDRGWQNMQPLMEGRDLDLYIENGNVFVYDGSLIYQILLQSE"
     gene            complement(9861..11450)
                     /locus_tag="VFS75_00780"
     CDS             complement(9861..11450)
                     /locus_tag="VFS75_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4677235.1"
                     /translation="MAKRPQLLPYAARPDFKVGRATELSGKNRAFYRFLEILPGAASW
                     LSLIAMVLASIYTPFFAAYFIIAFSVYWVLKTAFLSYHLRHNWRRLKVHMAMDWSAMI
                     ERFECSHLYHLVILPFYNEPEAVIDASLASLANARYDKKSMIVVLAREERAGEPARIA
                     AERMREKWSGTFGSFLVTAHPGDIEGELAGKGSNATWAAEEARRKILDPHGIRYNHTL
                     VSIFDIDTVVYPDYFNCLVWHFMTARRPLKSSFQPVPVFNNNIWEASALARVVAMSST
                     FWEMVQQERPERMATFSSHSVSFQALYEIGYWQTNMVSEDSRIYWNLLIANDGEYDVV
                     PLSYPVSMDANTAPTFWGTIKNIYKQHRRWTYGVENLVYLIFHFTKNKAIPLRRRIII
                     ALQQFEGYWSMVTNPIMLIILGWAPIFFGGRVFHETVLSYNLPIVVRDLLILAMFGLV
                     VSSMISLTLVPERPDGRGRHLWLVMALQWILVPFTMVVFSALPGLDAQTRLMFGRYMG
                     FWVTPKARDREEESEERAAVA"
     gene            complement(11537..11956)
                     /locus_tag="VFS75_00785"
     CDS             complement(11537..11956)
                     /locus_tag="VFS75_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677236.1"
                     /translation="MNAANLGMRSVSVFGEAMLVPADTIVLPEGFVAAAVSFDGKRSA
                     GRLYYGVRLTHANMRHVESRVREAEFQLYDVPPCVSWSEALKIEAVREQVVAVQQALA
                     QLYSLMLESYLKKNVISEAECLAFLRGKRIAILGSSR"
     gene            complement(12069..12142)
                     /locus_tag="VFS75_00790"
     tRNA            complement(12069..12142)
                     /locus_tag="VFS75_00790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12106..12108),aa:Thr,seq:tgt)
     gene            complement(12226..12651)
                     /locus_tag="VFS75_00795"
     CDS             complement(12226..12651)
                     /locus_tag="VFS75_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677237.1"
                     /translation="MDPFLTGLVVSALVALILNAFFLHEERTGKRVLGRLRAKADIGV
                     LRAGRQLRAALHYIGRDFLRQLFHYLFHRVLKAALALIRRIELALRNAIRVNKSLAKS
                     VDAERLERTKLEEIAFHKAATALSDEEKRERRERMLEGV"
     gene            complement(12656..13240)
                     /gene="tsf"
                     /locus_tag="VFS75_00800"
     CDS             complement(12656..13240)
                     /gene="tsf"
                     /locus_tag="VFS75_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU4677238.1"
                     /translation="MEITSAQLKELRDLTGISVMQCKKALEEAGGDMDKAKIILQKKS
                     GEIAAKKSDRALGAGVVGSYVHSTNEVAAMVLLSCETDFVAKNPEFIALARDIAMHVA
                     AQHPLYVRRDQVTEEAMTKAREVFAAEVADKPADIQEKILAGKLDAYFKDRVLMEQPF
                     IKDPDRTIGDLVTNAIQKFGENVVISDISRLSVK"
     gene            complement(13286..>13908)
                     /gene="rpsB"
                     /locus_tag="VFS75_00805"
     CDS             complement(13286..>13908)
                     /gene="rpsB"
                     /locus_tag="VFS75_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU4677239.1"
                     /translation="GTDIIDLEKTTELLEAAKSVLAEAGKAGKTVLFVGSKEEIAPVV
                     KAHAEAAEMPYVVNGWVGGMLTNFAEIKKRVQRLGDLTAQGESGELERKYTKKERVII
                     GRELDKLTHNFLGIQKMDRLPALLLVVDARHDSIAVAEARELDIPIVGIVSSDTNLRL
                     IDHPIVANDSLQASVTLILDELTKAYMSGREEFVPAKKEEVRKRDR"
BASE COUNT         2642 a         4288 c         4220 g         2758 t
ORIGIN      
        1 ccccacttct actgcagctt cacccctacc gcccgcaaat gccgcgacgc ggtatcgcga
       61 cgatctcggg gagcatcgtc ccgttgtcct tccggtaccg catgtccgcg acgtagtact
      121 ttcgtccgga gtcgtacgtg agccccgtgc cctcgaagtt ctggggccag atggtcacgg
      181 tgagcggggc gtgcgggtcg ggatcgatga tgtcgccgag ctcgatcgtg accgcgcccg
      241 acttcatgtc gatcgtgccg aaacgaacga cgccgggagc gccggaggcc gcgacctcgc
      301 ccgtcacccg gtagtcccag gtcttgtcgg gcgtctcgac gcggaagctc tttccgtcga
      361 cgttgaggtc tcgccgcacg ccgtcgatct ggacggtgag gacgagaccc gggccggcgt
      421 acttgaagcc gcgtacgtgc ttcttcacgt tgtgcgaggc gttccacgcc cacgacggct
      481 ggttcgttct tccctgcctg tcagcacgga acttcgctac ctccggcgtc tcgcagatga
      541 cgcggagctc gagcttcgtc ggtgtcgcga aggctgcgct cgagagcgcg accccaacga
      601 ggatagccgc gagaaacgac gtacgattca tgaaacctcc tatcggttgg ttgtggggaa
      661 cgaaatcgac ctacacgatt acacgtaaaa tatagtgtgt caagagaccg cgcgaaggat
      721 ggcgtcaaag gcgtcgaccg cgtgcgcggt gctgtcgatg cggacctgtg cttcacacgc
      781 gttcgtcatg atgcctccta acagcttccg ccgagcggta tggtcgtcga agatgcggta
      841 ccatggcgct tcgtactgga ggttcacgag attctggaac gcgagcgtga gcgtgccgcg
      901 atacgcacgt gcatgcgcgg cgtcccccgt cgccttcgcg acggcgtacg cggtcgcgag
      961 cccttccgtg tacacggcgt ccgacgatgc gtgcggactt ccgtactcgg gcgtctcggg
     1021 atcatagaat ctgccgatga actcctccat gtcctcgagc tcgaggagtt tgtcattgag
     1081 cgcgaagatg gcgttcgcgt accgcatgtc cttcgtcgcg ttaaagagat gcgagagcgc
     1141 gagcgtgtgc caggggacgt acgccggata gtagtgtgcg ctcatgtcgt cgacgtacgc
     1201 gccgatgtag tggtcctcgg ctcggcgcgc gtccgtaagg accgccacat ccttcacctt
     1261 ctccccatac tcgaggagcg cgagcgtcgc ctccccggaa tagaacgtga gaaggtagtc
     1321 tcggtcgtac gcgtagtcag gctccttgta ccacggttcg aaagaaccat cctcgtgcat
     1381 gagcgagaga atgccagccg cgagccgctt cgcctccgtc gcgtaacgcg cgaagtccgg
     1441 actcgcgacg aggacgcgaa ggagcatcgc gttcgcgccg agtttcgact tcccgtacgc
     1501 gtacacgtac gcctgctctc cgtcctcttt gtaccagttc tccatgatga cggagagatt
     1561 gcgctcgtga agcgtctcat aggacgaatc aaacgcggcc gcctcggcga ggacgcgtga
     1621 cgcgaggagc tgccgaatgt cgttgtcggc gttcgagatg gtgccgtttg ggtgttcctc
     1681 gtacgtgaag tagctcgtca cagggtcgag agaacggacg agcgacgaga acgcgagcgt
     1741 acgagcgtcc cgaaccgcct cagcgaacac ggcgtcgttc tccgtgatgc gcggatcggg
     1801 tacgaccttc gtacgcgcga cgcacacgac gtcagccgct gcaaggagcc agaacgcgag
     1861 cgcggcggcg gcgaggagaa cgaggacggc gacccaaaac cgtttcgaac gaaacatacg
     1921 catatgctag cacgctagga gcgaagcggc ttcgaggaga cgagcgatgc ctcgacgatg
     1981 aagcgatcat ccttcgtcgc gaaggagttg caggtcgcga gcgtgagctt cgcgacgccg
     2041 tgcgcgatgg ggacctcggc gctcgaggcc ttcgcctcgt agacccgatc gacggcgtag
     2101 acgtactcgg tgtccttcga acggaggcga atggtgtctc cccacttcag cttctggatg
     2161 ccgttgaatg cctggaagtt cttattgtga acgacgggaa ggtagcttga gtgtccgagg
     2221 aggaagatgg ttcccgtatg ctcgaagtcc gcggagtcgg gatgtcgtac gacgcccgag
     2281 aggagggcgg tatcgaggac ggacacgtcg tccgacgtcg gattctgtat agtgagttcc
     2341 ttatcgagcg agtcgaaggt gatggacacg ggatacgggt cgaccgcgac cggcgcgggc
     2401 tccgcaggcg tcgtcgtcgc cggtttcgtg agcgcctcct tcgccgcatc gttcgtgccc
     2461 ggtttctccg gaatgaaatc gatgaggtag aggagcccgt acgtcaccgc gataatgccg
     2521 acgagcacga agaagaacac gacacgttgc tcgagcttcg atgcgtaaag ttcctcgatt
     2581 cggccctccg tgtgatccat atgagattga agtaaacctt gattatgcga tttgtcaact
     2641 agccatttaa aaatggaagc atagaatgcc tccgaatagt actctcttcg gtcattatcg
     2701 gcctattgac atccacttgt tcaagtggtt atatcttcgc catgaaggaa acacaccagt
     2761 accgcattct catgataaaa accctcgcca tcatagggtt tttggtgacc ttgggcctcg
     2821 tcgtatggct catcgtcgcg gggctccgcc ttatccccgg cgcgttctcg tcactcgcct
     2881 cgatcgccga gagcatcaag aactaccgac cgctcccctc tctcgagctc accctcgaga
     2941 agacggccgt cgactccggt gagtcgttcg aactccattg gaccgacatg ggcaccgaag
     3001 gtacgttcca cttctcgtac ggatgcaccg acggcgtcgg cctcggcgtg cgcgggcagg
     3061 atggcgtctt cgttccgata cgttgtacgg actcactcgg cctccccgcc gatgtccatg
     3121 gcctcttcct cagcgtcacc tccgatgcga tgcgcgtcgc cgatgtaccg ctcacacttt
     3181 catacgagaa cgacgcgaag cacgtgacga aggaagcgaa ggcgacggtg accgtcgcga
     3241 acgcgaacat cggcgagaag ccggcgacca cgaccccggt cgtcacgcca tcaccaaagc
     3301 ccgcgacgac gaccgcgacg tcctcgaaac ccgtgacccc gacacccgca ccaaagccgg
     3361 tcactcgcgt cgtctatccg ccctcggacc ccaacggata taccgatctc gccgtcacga
     3421 ctctcggaag cggcatcatc cgaaacggtg cgttctcgtt cacggcctcg tacgagcgcg
     3481 acgcgaagaa cgccattcgc ttcgacgtga agaacgtcgg tacgaagacc tcggacgagt
     3541 ggtacttcaa gacactcctc ccctcgggcg agacctacac gtcgccgacg caggcgccgc
     3601 ttaagccgta cgagcacatc gagttcacgc tcggattcga cctcgacacg aacgcgaacc
     3661 tcgtcaccat cgtgaacagc gccttcaccg acagggacgt taacgcgaag aacgacagcg
     3721 ccgcgtggtc ggtcgtcgtg aagtaaccat cgaaacgaca cgaaaaatgc cgcggcagag
     3781 ccgcggcatt tttcaatgca attagctaca cgtcgaggtt cgccatgcgc gcgttcgact
     3841 ggatgaacga tttccgactt gagacgtcgg tccccatgag aatgtcgaac accttatcgg
     3901 cggcctgcgc gtcgaggatc gatacctgct tgaggacccg gttctcggga ttcatcgtcg
     3961 tctcccagag ctcctcggcg ttcatctcac cgagaccttt gtaacgctga atgcgcacct
     4021 tcttcgactt ggcggcttcc tctttctctt cgacctcggc cgcttcctcc gactccgctt
     4081 caggctcgcc gtcggactca acgtcctcga cgaccgcatc ctctccgagg agcgtcatct
     4141 tctcctcgtc gctgtacgcg tagagcacct ccttccccct ctgtatcttg tagagaggcg
     4201 gctgcgcgat gaagaggtac ccaccgtcga tgatcggctt gaaatagcgg tagaagagcg
     4261 taaggaggag cgtgcggatg tgcgcaccgt cgacgtcggc atcggtcgcg atgatgatct
     4321 tgtggtagcg gagcttcccg atgtcgaaga cgtcaccgat cgccgtaccg agggcgacga
     4381 cgaggttctt cacctgctcg gaaccgagca tgcggtcgag acgcgcacgt tcgacgttga
     4441 gtatcttgcc acgaagcggc aagatagcct gcgtccgcct atcgcggccc atcttcgcgg
     4501 aaccgcccgc ggaatctccc tcaacgatga agagctcgga ttcctcggcg ctcttcgact
     4561 ggcagtcggc gagcttccct gggagcgtca tcccctcgag ggcgcccttc cgaagcaccg
     4621 aatccttcgc ggccttcgcg gccttacgcg ccttcacggc gataacgacc ttgttcacga
     4681 tcgccttcgc gtcgtcgggg ttctcctcga ggaacgtcgc gaacgcgtcg ccgaagacgc
     4741 tctcgacggc gcttcgcgct tcgacggatc cgagcttcgc cttcgtctgt ccctcgaact
     4801 ggatctccgg catcttcacc gagacgacgg cggtgatccc ctcaagaacg tcatcgccgg
     4861 tgaaaccggc gtccttctcc ttcaccgaga agaagttgtt cttcttcgcg tagttgttca
     4921 atgttcgcgt aagcgcggtc ttgaacccgg tcacgtgcgt gccgtgctcg ggattgtaga
     4981 tgttgttcgc gaacgcgctg atgcgcgccg tgatgtcgtc gacgtactgg agcgcgacct
     5041 cgaccgagag caccttcgcg tcgacgtcct gcttctcgac gtagaagatg cggtcgtgga
     5101 cgggcttctg atactggttg ttgaacgcga cgagcgactt caatcctccc tcgaagtaga
     5161 agtgctcgtg cggcacctcg agcgcctcgt ccgtgagata ctgcttcccc tcgacgtcga
     5221 gcttccgctg tgcctcctcg ctcatctcac gtgcgtcgat gatcgatatt ttgagtccct
     5281 tcacgagata cgcctgctgg cgaaggtgcg tcacgatgcg gcgccactcg tacttcacct
     5341 cctcgaagat ggtgtcgtcc gcctggaacg tgatgatggt tccctggagt ttcgacggcc
     5401 cgaccttctt caccttcgcc ttcggcttac cgatgtggta ctcctggatg tagtacgcac
     5461 cgtctcggtg cacctcggcg atgacgtgcg aggagagcgc gttcacgacg gagacgccga
     5521 cgccatggag gccgccggag accttatatc cctcaccgcc gaacttaccg cccgcgtgga
     5581 gggtcgtcat gatggtctcg agcgcagata ccttcgtctg cttgtgtatg tcgacgggaa
     5641 tgccacggcc gttgtcgacg acgcgaacgt aattgcccgg aagaagcgcg acctcgatcc
     5701 tgttcgcgaa gccgcccatc gcctcgtcgc gacagttgtc gaacacctcc caaatgaggt
     5761 ggtggagtcc atcgggaccg gtcgttccga tgtacattcc cgggcgcttt cggaccggct
     5821 cgagtccctc gaggacctgg atggaatccg cgctatacgt gctcttcgct gtatcttttt
     5881 ttgccatgcc cgaccgaaaa tgtgcgtcaa taataaaacg agctcaaagg gctctgatag
     5941 gaaaagtata gcagatttcg gccattttgt cccccttccg tgggcacaaa aaagcgccga
     6001 aacggcgcct gtttcggggt cgtcggcgac cgcttaccgt acctcgtagc cgcgctccgc
     6061 gagcataccc gaaattcgct ccatgattac gccgacctcc tcgtcggtga gcgtccgctc
     6121 cttcgactgg aatacgagcc ggaacgcgta cgagacccgc ccgtccttct tgaactcgtc
     6181 gaactgcgag agccgcacga gaaggtctcc cgctccctct cggagaaccg cctcgatatc
     6241 cgccgcggcg accgtctcct tgacccaaag cgcgatgtcg cgggagacga acgggtactc
     6301 tgagaacggc cggaaccgaa cgtcggcggg aggtgtgaac ggttcgtacg cggcgggcga
     6361 cggaaggttc gcgatcaccg aggtaaggtc gcactcggcg acgccgtcat gaacgtccgt
     6421 ggtaagcggc gcgccgaggg acgattcgag cgcggtgagc gcttcgcgga ggcgcgcgtc
     6481 gtccgcaggc gcgtatcccc ccgccttcgt ccgcacgccg agcgcgagcg agacgtgctc
     6541 cgtgacgtcc gccccgtcat cggtgcggca aaacacggtt cctatctcga agagctgaat
     6601 acgcgacacg ccgaggaggt ccatgtatgg cgcgttccga agaagcgcct ccttcacgtt
     6661 cttccgaagg tcgctccgga ggtatccctt gtcgctcgcg agcgcgttgc ggagtttgat
     6721 ttcgtccttc ttcctgaaag acgacgtgat tatctccgag aagccgagcc caacgagggt
     6781 ctcccgaata cgctccgcat agtagtggcg tgcgttcacc tcggcaagcg gtgcaggcgg
     6841 cggcatgata gcgtccacgt gctcgtatcc gtagacgcgg ccgatctcgg cgatcacatc
     6901 ctcgggaatg acgatgtcgg tccgttcgaa aggcgctatg accgcccacg cgtcgccttt
     6961 tcgctcgtac gaaaaaccga gccgtcgaag tatgtcctcg atggcctccg caccaagcga
     7021 cataccgagg agtccctcga tgtgcgcgag ggagacatcg acacgcgggt tctcgcgtgg
     7081 cgtcgggtat gcgtccacgt acccctcgag cgtccctccc gcgatatcga ggatgagcgc
     7141 gaccgccgcc gtgagaccgt acgcggcgag ttcaggcgac agatcgttct cgaaccgctt
     7201 cgacgcgtcg gtcggaagac ggagcgattg cgccgcgcgg cgggtcgtcc tcgggtcgaa
     7261 gttcgccgct tcgaggatga tcgcggtcgt cgaggcgtct atctccgcga gttgcccgcc
     7321 ctttataccc gcgatgccgg ctggcgcgtc gcttttcgcg tcgacgatga gctggacctc
     7381 gggcgaagcc gtgtacgtct cgccgccgag gatggtcacc tcctcgcctt cccgtgccat
     7441 gcggacgcca aagcgccaca cgcctccctc gtgggggaac ttctcggcat cgtatgcgtg
     7501 aagcggctgg ccgagtccga gcatgacgta gttcgtcgca tcgacgacgt tgttgatgga
     7561 acgctgcccg agcgcggcga gacgggccct cagccactcg ggcgaatcct tcacggtgag
     7621 acccgtgatg cgagccgcgc taaagcggcg acaacgggat gggtcttcga tggagacggt
     7681 aagactgtcc gtctcgggcg taagcgagac gggagtcgta agcgggtccg cctgaagcgg
     7741 cgtgccggta agcgtcgcta tctcacgcgc gatgccgcgg tgcgagaggc agtcgctccc
     7801 gcgatccggg agcaccttca cgtcgatgac ctcgttcccc gcgatctctt cgatgccgtc
     7861 gatctcgaac gcatggaacg tgagcaggtt ctcgagttcg ggaagcgacg ggagcgcatc
     7921 accggtatag tccttgagcc aatcgtagag tactttcata tcaaaactga ttcacgaagc
     7981 gaaggtcgcc gctgtagagg tcgcgtacat cgtcgaactc gtacttcatg aagccgagac
     8041 ggtcgatgcc cataccgaac gcgaaccccg agtaggtcgc ggggtcgatg ccggacccct
     8101 caaggaccga cgggtggacc atccccgcgc ccatgacctc gatccaccga cccttcaatt
     8161 tcgacgcgcc ggaggtgatg cgcatgtcga cctcgacgcc cggctcgacg aacgggaaga
     8221 agctcggccg aaaccgaacc tcgacgtcgc ccttgaagaa cttctcgaag aaatactcga
     8281 tggttccctt gagatgcccg aggtgaacgc ccttgtcgac gtaaagaccc tcgagctggt
     8341 agaactgcgc ttcatgagtc gcgtcggtcg cctcgttacg gaagaccttc cccggcacga
     8401 tgacgcgtat cggcggcgtg tgcgtctcca tgtatctcgc ctggaccggc gaggtgtgcg
     8461 tgcgaaggac ggtcggctct ttgacgtcct tagatgagaa ccagaacgtg tcctgcatat
     8521 cgcgagatgg atggtccgcg gggacgttca ggcggtcgaa gttgtagcgc tcgcgctcgg
     8581 cctcgggccc ctccgcgaag acaaacccga tctcggcgaa gatcgcgttg atctcggcga
     8641 tcatactgct tatcgggtgc ctatgtcctc gctcgctcgc gttcatactt attcgctctg
     8701 taagagtatt tggtagatga gactcccatc gtacacaaag acgttgccgt tctcaatata
     8761 gaggtcgagg tctctcccct ccatgagagg ttgcatgttc tgccagcccc ggtcgtctat
     8821 ctcgacgacg tagatgccat cctcgagaac ctcgaggatg aggtcggtcg agcccggata
     8881 gaagtcgaag gctcggagcg tctcgccctt tcggtccatc ttcacggtcg ggtcgcacgc
     8941 cgccccggcg gggactgcct gaaccgggtg catgacgagc ccgtccggat cgacgtcctg
     9001 tcgcaccgcg ggcgtcgtga ccgcggacga gatggcgggc gtggacgtgc tatagggcgg
     9061 gaaggtcggc gcgcagtaat agtacggcat ctgttcgaac ggaccgaccc acgtcgcgaa
     9121 gaggtcatca ccctcgagag cgagccgaac gcccgaggag atcttggtcg aggtggcctc
     9181 gtcggtcgtc gttgctcctc caccagtgag aaggccgcga aggtactcgg cggtcgtctc
     9241 acggctcgcc gtcgaggtgg aggtcgacgc gaagtgcgcc atgagagacg tgtactcctg
     9301 gtcgagcttg aacgacgcgg cggtcggctt cgtcgtcgtc gcgacgtacg cgttcacgac
     9361 gtgcgggtac gcgatctcgc tcttcacgac cggtgacccc gtcgccgtat cccacttcgc
     9421 gatgagacgc gcgtccggga tcaagggcat gttgaaggcc gccgcctcgg tgacgagatg
     9481 ctccgacacg ggaagttcct tcacccacgt gtggtgtccg tccttctgca cgtgaacgcg
     9541 gtgcgtccca acgtcgaggt tctgcgcata gaacgcttgt cgaaacgcgc gtgtctcgcg
     9601 gacgagctcg tcgtcgatat agatctgcgc ccccgtgcgg tctgcggcga cgtagatgcc
     9661 gcccgtcgaa acgatgttcg tcggcttcgc gaagtcgaag cggtagccgg tcgcatagag
     9721 gtacaaaaac ggcaaaacga cgagaaagac gatgacgaga aacgcgaaga ggttccgccg
     9781 tgaattgtac gggaggggtt ttacgtactc actttgctcc ataagtctgt agggtaccac
     9841 gatatcgcgg aaatttccgc tcacgcgacc gccgctcgct cctcgctctc ctcttcccta
     9901 tcgcgcgcct tcggcgtcac ccagaacccc atatagcgtc cgaacatgag ccgcgtctgc
     9961 gcatcgagcc ccggaagcgc gctgaagacc accatcgtga acggaacgag tatccactgg
    10021 agcgccatga cgagccagag gtgcctaccg cggccatcgg gccgttcggg aacgagcgtg
    10081 agcgagatca tcgatgagac gacgagcccg aacatcgcga gaatgaggag gtctcgaacg
    10141 acgatcggca gattgtacga gagaaccgtc tcatggaaga cgcggccgcc gaagaagatg
    10201 ggcgcccacc cgaggatgat gagcatgatc gggttcgtca ccatcgacca gtacccctcg
    10261 aattgctgga gcgcgattat gatacgccga cggagcggga tggccttgtt cttcgtgaag
    10321 tggaagatga ggtacacgag gttctcgacg ccgtacgtcc accgccgatg ctgcttgtat
    10381 atgttcttga tggtccccca gaaggtcggc gcggtgttcg cgtccataga gaccgggtac
    10441 gagagcggta cgacatcgta ctcgccgtca ttcgcgatga ggaggttcca gtagatacgc
    10501 gagtcctcgc tcaccatatt ggtctgccag tagcctatct cgtagagcgc ttgaaacgag
    10561 accgagtggg acgagaacgt cgccatgcgc tcggggcgct cttgctgaac catctcccag
    10621 aaggtcgagg acatcgcgac gacgcgagcg agagccgacg cctcccagat gttgttattg
    10681 aaaacgggga ccggctggaa cgagctcttg agcggtcgcc gtgcggtcat gaagtgccag
    10741 acgaggcagt tgaagtaatc cgggtacacg accgtatcga tgtcgaagat ggagacgagc
    10801 gtgtgattgt agcgaatgcc gtgcgggtcg agtatctttc ggcgtgcctc ttcggcggcc
    10861 cacgtcgcat tagagccctt cccggcgagc tcgccctcga tatcaccggg gtgcgcggtc
    10921 acgagaaaac tcccgaacgt ccccgaccac ttctcgcgca tgcgctcggc cgcgatgcgc
    10981 gcgggctcgc ccgcgcgctc ctcgcgcgcg aggacgacta tcatgctctt cttgtcgtat
    11041 cgcgcgttcg cgagagacgc caaggaggca tcgatgaccg cctcgggttc gttgtagaac
    11101 gggaggatga cgaggtggta gaggtgcgaa cactcgaagc gctctatcat ggcgctccaa
    11161 tccatcgcca tgtggacttt gagccggcgc caattatgcc ggagatggta cgagagaaac
    11221 gcggtcttca gcacccagta gaccgagaac gcgatgatga agtacgcggc gaagaacggc
    11281 gtgtagatgg acgcgagaac catcgcgatg agagagagcc acgacgccgc tcccgggagt
    11341 atctcgagga atcggtaaaa cgcacgattc ttgccggaga gttcggtcgc tcgtcctacc
    11401 ttgaaatcgg gtcgggctgc gtatgggaga agctgcgggc gttttgccat gacggagtcg
    11461 agtatagccc caaatgagcc attttgaaag cgccggcata atcgaaaaag gggtacgcgt
    11521 tcgcgtaccc cgacctctac cgcgacgaac cgagaattgc tatccgcttc ccgcggagga
    11581 acgcgagaca ctccgcttcg gagatgacgt tcttcttcag atacgattcg agcatgagcg
    11641 agtacagctg cgcgagcgcc tgctgtaccg cgacgacctg ctctcgcacc gcttcgatct
    11701 tcaaggcttc gctccatgaa acgcacggcg gaacatcgta caactggaat tccgcttccc
    11761 tgacgcgcga ctctacgtgc cgcatgttcg cgtgcgtgag ccggacgccg tagtacaacc
    11821 tccccgcgct tcgcttccca tcgaagctta ccgccgcggc gacgaatcct tccggcagaa
    11881 cgatggtatc cgcgggcacg agcatcgctt ccccgaagac gctcacggac ctcataccga
    11941 ggttggctgc gttcatcgta aacctcccta tgtgtaaccc gtcgtacggt actcctctta
    12001 gaaagaggtg tcaaaaaagc gaacggactg cctctgtaag aggatacggt ttacccttca
    12061 agactgtgag ccgcctctca gattcgaact gaggacctac ggtttacaaa accgttgctc
    12121 taaccagctg agctaaggcg gctcacagtc ttgaacgaaa cttttcgtca gtatcccata
    12181 ctgacgaaac tccacccagc tccgctgagc cgctctggcc ttgaatcaga cgccctcgag
    12241 catccgctcc ctgcgttcac gcttctcctc atcggagagc gcggtcgccg ccttgtggaa
    12301 ggcgatctcc tcgagcttcg tccgctcgag gcgctcggcg tcgacgcttt tcgcgagcga
    12361 cttgttcacg cggatggcgt tacgaagtgc gagctcgata cggcgaatga gcgcgagcgc
    12421 cgccttgaga acgcggtgaa agaggtagtg aaagagctgc cggaggaagt cacgtccgat
    12481 gtagtggagt gccgcgcgca attgccggcc agcacggaga acgccgatgt cggccttcgc
    12541 gcggagccgc ccgaggacgc gcttccccgt tcgctcttcg tggagaaaga acgcgttgag
    12601 gataagcgcg acgagcgcgc ttaccacgag accggtgagg aacgggtcca tgcggctatt
    12661 ttaccgagag acgggagata tcgctaatga cgacgttctc gccgaacttc tggatggcgt
    12721 tcgtaacgag gtcgccgatg gtgcggtccg ggtccttgat gaacggctgt tccatgagga
    12781 cgcggtcctt aaagtacgca tcgagcttcc ccgcgagtat cttctcctga atgtcggccg
    12841 gtttgtccgc gacctccgcg gcgaacacct cgcgggcctt cgtcatcgcc tcctcggtca
    12901 cctgatcgcg gcgaacgtac aggggatgct gcgccgcgac gtgcatcgcg atgtcgcgcg
    12961 cgagcgcgat gaactcggga ttcttggcga cgaagtccgt ctcgcatgaa aggagcacca
    13021 tcgcggcgac ctcattcgtc gagtggacgt aactaccgac gacgccggcg ccgagcgcgc
    13081 ggtcggattt cttcgccgcg atctcgccgc tcttcttctg gaggatgatc ttcgccttgt
    13141 ccatgtcgcc gcccgcctcc tcgagggcct tcttgcactg catgaccgag ataccggtaa
    13201 gatcacgcag ttccttaagt tgtgccgatg tgatttccat ggtcaaaaga acgccctgat
    13261 gatgataaaa cgcgaagaga ccgttttacc ggtctcgctt tcgtacctcc tccttcttcg
    13321 cggggacgaa ctcctcccta ccgctcatgt acgccttcgt cagctcgtcg aggatgagcg
    13381 tgacgctcgc ctggagagaa tcgttcgcga cgatcggatg gtcgatgagc cggaggttcg
    13441 tgtccgagct cacgatgccg acgatcggga tatcaagctc gcgcgcctcg gcgacggcga
    13501 tcgaatcgtg tcgcgcgtcg acgacgagaa gaagcgccgg gaggcggtcc atcttctgga
    13561 taccgaggaa attgtgcgtg agcttatcga gctcgcgtcc tatgatgacg cgctccttct
    13621 tcgtatattt gcgctcgagc tcgcctgact caccctgcgc ggtcagatca ccgagacgct
    13681 gaacgcgctt cttgatctcc gcgaagttcg tgagcatgcc gccgacccat ccgttcacga
    13741 cgtacggcat ctccgccgcc tcggcgtgcg ccttcacgac gggagcgatc tcctccttgg
    13801 agccgacgaa gaggacggtc ttccccgcct ttcccgcctc ggcgagaacc gatttcgccg
    13861 cttcgaggag ctccgtcgtc ttctcgaggt cgattatgtc cgtgccct
//
LOCUS       DASTSP010000018         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_929796, whole genome shotgun sequence.
ACCESSION   DASTSP010000018 DASTSP010000000
VERSION     DASTSP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929796"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VFS75_00810"
     CDS             <1..1169
                     /locus_tag="VFS75_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4677240.1"
                     /translation="GTLDQKKVELFLNELKDGNDALKVVTYKQIDSRTFDTELVNAIA
                     EGRSPDLVILPSSLLVSYRTKLQALSAETLDPRVFRDTYVDGAEVFLMSDGTYGLPFA
                     VDPLVMYWNRDLFSSGGLATPPSTWESLVTETVPALVRTDPDLNITQSAIAFGEYANV
                     EHAKDVLTMLLMQAGTTIVTESDGRYEVTLLRKNASALSPGEAALTFYTQFVVPGKDL
                     YSWNRAKRLDRDMFLAGDLALYFGLGSERSELEAENSNLNFDVAMVPEGEGATVKRTS
                     GEFYAFVLPRASKNLQGAYALAKYLGNAENGPALADAFGFAPAHRTAYAGSVSDPFKQ
                     VVYQSALIARAWLDPSPSGSADVFERMVEDYTSGRARLSAVLSDAASELQALFK"
     gene            1172..1543
                     /locus_tag="VFS75_00815"
     CDS             1172..1543
                     /locus_tag="VFS75_00815"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEU4677241.1"
                     /translation="MSILRRYARFAYVFVALVPSFAFAQNTTGLDAGKCPKGALCNPL
                     HATSISELIANIIDVILVFATPLIVLMIMYAGFLFVTARGNEGQVESAKKALLWSVVG
                     GVIVLGARIISTVIEGTITSF"
     gene            1545..1868
                     /locus_tag="VFS75_00820"
     CDS             1545..1868
                     /locus_tag="VFS75_00820"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677242.1"
                     /translation="MRPRLIQLTAIALASLPSIALAASTPTNFRGLVDVILGIIKIVV
                     TLVFGLTFLYFMWTLIRTWIVHGGDAGSIEEGKKTLVVAVIALVIMISVWGIVGLIRQ
                     SLFGI"
     gene            1980..2351
                     /locus_tag="VFS75_00825"
     CDS             1980..2351
                     /locus_tag="VFS75_00825"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677243.1"
                     /translation="MKIIAYLLPLVLIVPMSALAAGEGQLGTVSTFITNIVNFINRTL
                     VPFIFALAFLVFLWGVFKTFILGGSDEGKQEEGKKLMMYAIAGFVIMVSLWGIVNLVA
                     KGFGFTNEDITPLIPKTPDVH"
BASE COUNT          476 a          790 c          700 g          546 t
ORIGIN      
        1 ggggcacgct cgaccagaag aaagtggaac tttttctcaa cgaacttaag gacggtaatg
       61 atgcgcttaa ggttgtaaca tacaaacaga tcgattcacg cacgttcgat accgagctcg
      121 tgaacgctat cgcggagggg cgctcacccg acctcgtcat cctgccgagc tcgctcctcg
      181 tctcgtatcg tacgaagctt caggcgctct cggcagagac cctcgaccca cgtgtcttcc
      241 gcgatacgta cgtcgacggc gccgaggtgt tcctcatgag cgatggcacg tacgggctcc
      301 cgttcgccgt cgacccactc gtcatgtact ggaaccgtga cctcttctcc tcgggcgggc
      361 tcgcgacgcc gccgtcgacg tgggagtccc tggtcaccga gaccgtgccc gcgctcgttc
      421 gcaccgaccc agacctcaac atcacgcaga gcgccatcgc gttcggcgag tacgcgaacg
      481 tagagcacgc caaggacgtt ctcacgatgc tcctcatgca ggcggggacg accatcgtga
      541 ccgagtctga cggacggtac gaggtgacgc tccttcgaaa gaacgcctcc gcgctctcgc
      601 ccggcgaagc ggcgctcacg ttctacacgc agttcgtcgt gcccgggaag gatctctatt
      661 cgtggaatcg ggcgaagcgc cttgaccgcg acatgttcct cgccggcgac ctcgcgctct
      721 acttcggcct cgggagcgag cggagcgagc tcgaggcgga gaactcgaac ctcaacttcg
      781 acgtcgccat ggtgccggag ggcgagggag cgacagtgaa gcgcacgtcg ggcgagttct
      841 acgcgttcgt ccttccgcgt gcgtcgaaga accttcaggg cgcgtacgcg ctcgcaaagt
      901 atctgggaaa cgcagagaac ggaccggcgc tcgcggacgc tttcggcttt gcgcccgcac
      961 accgtaccgc gtatgccggc tccgtgagcg acccgttcaa gcaggtcgtc taccagtccg
     1021 cgctcatcgc gcgcgcgtgg ctcgacccgt ccccctcggg gagcgcggac gtcttcgagc
     1081 gcatggtcga ggactacacc tcgggtcgag ctcgcctaag tgccgtgctc tctgacgcgg
     1141 cgagcgaatt acaagcgtta ttcaaatgac catgtctatc cttcgccggt acgctcgctt
     1201 cgcctatgtt ttcgtcgcgc tcgtcccgtc tttcgcgttc gcgcaaaata ctaccggatt
     1261 agatgctggc aagtgtccca aaggagcgct ctgtaacccg ctccacgcga cgagcatttc
     1321 ggaacttatc gcgaacatta tcgacgtcat cctcgtcttc gcgacgcctc tcatcgtcct
     1381 catgatcatg tacgccgggt tcctcttcgt gactgcgcgc gggaatgagg gtcaggtcga
     1441 gagcgcgaag aaggcgctcc tctggtcggt cgtcggcggc gtcatcgtgc tcggcgcgcg
     1501 catcatctcg accgtcatcg aaggcaccat aacttcgttc tgatatgcgc cctcgtctta
     1561 ttcaactcac cgcgatcgca ctggcgtctc ttcctagcat cgcgcttgcc gcgagcacac
     1621 cgacgaactt ccgcggcctc gtcgacgtca tcctcggtat catcaagata gtcgttacgc
     1681 tcgtcttcgg cctcacgttt ctctacttca tgtggacctt gatccgcaca tggatcgtcc
     1741 acggcgggga cgcggggagc atcgaggagg ggaagaagac gctcgtcgtc gcggtcatcg
     1801 cgctcgtcat catgatatcc gtctggggta tcgtagggct catccggcaa tcgctcttcg
     1861 gaatataacc attttcgtat ccacagtact accccccggt acttcccata aaggctagaa
     1921 taggtacaag gttcttaata tcgttaatca tatcccacgt tttacagcgt ttacacagta
     1981 tgaaaatcat cgcttactta ctcccgctcg ttctcattgt gccgatgagt gcgctcgccg
     2041 caggcgaagg ccagttaggt acagtatcga cgttcataac gaacatcgtc aacttcatca
     2101 accggacgct cgttccgttc atcttcgccc tcgcgtttct cgtcttcctc tggggcgttt
     2161 tcaagacgtt catcctcggt ggctccgacg aaggaaagca ggaggagggc aagaagctca
     2221 tgatgtacgc gatcgcgggg ttcgtcatca tggtctcgct ctggggcata gtgaatctcg
     2281 tcgcgaaagg ctttgggttc accaacgaag acataacccc tctcattcct aagacgccgg
     2341 acgtacacta gcctatgcag gcgtacgcgg cagattcagc ggacgccgta gcggaggcgc
     2401 atagctttct gactcggctc gagaacgcga ttctctttcc gctcatgacc ctcatgatgg
     2461 ctgtcgcgtt ccttatcttc ctttggggag ggtacgagta catcgcgaac gc
//
LOCUS       DASTSP010000019         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_485014, whole genome shotgun sequence.
ACCESSION   DASTSP010000019 DASTSP010000000
VERSION     DASTSP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485014"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2464
                     /gene="uvrA"
                     /locus_tag="VFS75_00830"
     CDS             <1..2464
                     /gene="uvrA"
                     /locus_tag="VFS75_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4677244.1"
                     /translation="AEGQRRYVESLSSYARQFLRQMQKPDVDEISGLSPAISIDQKSA
                     SRNPRSTVATITEIYDYLRILYARVGQPYCLDTDVPIKKLSQDEILAIVLKSIEDREI
                     RRVKSNDVRKVLGVEVSSARVSIFAPVVVGRKGEYYQLLYDLLGKGYEVVRIDGEIKQ
                     LREKIVLTKMKRHDIDVLIDEIYVSEFENSPKNAKERLSEAVELALRESNGLVKVRTP
                     DGDERMLSAKFICPVDGSSFPEVEPRLFSFNSPYGACPTCNGLGTVGVFNTDVCPACN
                     GARLRPEALRVYLGGDGKGDLGKNIVDFTNMTIEEAASFVAGLKLSKKDQDIAWPALR
                     EIIERLDFMINVGIEYLTLNRRANTLSGGEAQRIRLASQLGSGLVGAMYVLDEPTIGL
                     HQRDNERLIKTLTDLRDLGNTIIVVEHDEDTIFAADYLVDIGPGAGVHGGEVVVADYL
                     DTLLSAPKNTSKSVTLDYLRGDRRIPVPETRRVSEKGSVKIRGGKAFNIKNLNVDIPL
                     GRLIAVTGVSGSGKSTFMYEILYRNLRSRLDRKYRTPHTYNAASVTGTEYLGRAILVD
                     QSPIGRTPRSNPATYTGAFTYIRDLFASTSEARARGWKSGRFSFNVKGGRCEACQGNG
                     EVAVEMHFLPTVYVTCDVCDGKRFTKETLEVRYKGKNIDDVLHMTIEEADAFFIDVPA
                     IHERLQSLIDVGLGYLELGQSATTLSGGEAQRVKIASELYRPHTQKTIYLLDEPTIGL
                     HYEDVRKLIEILDELVKRGNTVMVIEHNLDFIKACDYVIDIGPEGGEKGGDVVAKGTP
                     EEVAKNPKSYTGTYLKKSLSHG"
     gene            2457..3689
                     /locus_tag="VFS75_00835"
     CDS             2457..3689
                     /locus_tag="VFS75_00835"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU4677245.1"
                     /translation="MDRAKFARMKLPDAPGVYFFIGPRKEILYIGRATSLLHRVKSYF
                     DDDIASKRSPAIEKMVRESVRIDVEETDSVLEAIILEVNLIRTHKPRYNTLSKDDKSY
                     NHVAITDEEFPRVLVLRGKDLRDVKHEWSYSHVYGPFPSGKLLRDALRIIRRIFKFYD
                     VKRPIREKMSKVLRGTIDFNRQIGLYPESTSPDAYQETIRHIRLFFEGRKRTVIAELK
                     RDMTRFAKQERFEEAGEAKRKILALEHIQDVSLLRDDLRAYRDDRSMRIEAYDIAHLA
                     GTHMVGVFTVVEGGKPDPSSYRKFNVRGFTASNDPGALREVLERRLRHEEWRIPDLIV
                     VDGNAVQKRVAERAAKAAGVVVPVAAVVKDERHRPERILGPRHIVEARAEEILLANAE
                     AHRFAIAFHRRKRGKLPE"
BASE COUNT          834 a         1198 c         1155 g          673 t
ORIGIN      
        1 cgcggaaggc cagcggcggt acgtcgagtc actctcgtcc tacgctcgtc agtttctcag
       61 gcagatgcag aagcccgatg tcgacgagat cagcggcctc tctccggcga tatcgatcga
      121 ccagaaatcc gcatccagaa acccgcgctc gaccgtcgcg acgattaccg agatatacga
      181 ctacctccgc atcctctacg cgcgcgtcgg ccagccgtac tgtctcgaca ccgacgtgcc
      241 aatcaagaag ctctcacagg acgagatact cgcgatcgtg ctgaagtcca tcgaagaccg
      301 cgagattcga cgcgtgaagt cgaacgacgt gcggaaggtt ctcggcgtcg aggtgagttc
      361 cgcgcgggtc tcgatattcg caccggtcgt cgttggccga aagggcgagt actaccagct
      421 tctctacgat ctcctcggca aggggtacga ggtcgtccgt atcgacggcg agatcaaaca
      481 gcttcgcgag aagatcgtcc tcacgaagat gaagcgtcac gatatagacg tcctcatcga
      541 cgagatctac gtgagcgagt tcgagaactc accgaagaac gcgaaggagc gtctctccga
      601 ggcggtcgag ctcgcgctcc gcgaatcgaa cggtctcgtg aaagtccgaa cgcccgacgg
      661 cgacgagcgc atgctctcgg cgaagttcat atgccctgtc gacggctcgt cgttcccgga
      721 ggtcgagccg cgcttgttct cgttcaactc gccctatggc gcgtgtccga cgtgtaacgg
      781 cctcggtacg gtcggcgtct tcaatacgga cgtctgtccg gcgtgtaatg gcgcgcggct
      841 ccgtcccgag gcgctccgcg tatacctcgg cggcgatggc aagggcgatc tcgggaagaa
      901 catcgtcgat ttcacgaaca tgacgatcga ggaggccgct tcgttcgtcg ccgggctcaa
      961 gctctcgaag aaggaccagg acatcgcgtg gccagcgctt cgcgagatca tcgaacgtct
     1021 cgatttcatg ataaacgtcg gtatcgagta cctaacgctc aatcgccgcg cgaacacgct
     1081 ctccggcggc gaggcgcagc gcatccgcct cgcgtcccaa ctcgggtccg gtctcgtcgg
     1141 cgcgatgtac gtcctcgacg agccgaccat cggacttcat cagcgcgata acgagcgtct
     1201 catcaagacg ctcaccgacc tccgtgacct cgggaacacc atcatcgtcg tcgagcatga
     1261 cgaggacacc atcttcgccg ccgattatct cgtcgacatc ggcccgggtg cgggcgtcca
     1321 tggcggggaa gtcgtcgtcg cggactatct cgacacgttg ctttccgcgc cgaagaacac
     1381 gtcgaagtcc gtgacgctcg actacttgcg cggcgatcgg cggatccccg ttcccgagac
     1441 gcgacgcgtc tcggagaaag gctcggtcaa gatacgcggc ggtaaggcct tcaacatcaa
     1501 gaacctcaac gtcgatatcc cgctcggccg cctcatcgcg gtgaccggcg tctcggggag
     1561 cggcaaatcg acgttcatgt atgagatact ttaccggaac ctccgttcgc ggctcgaccg
     1621 aaagtatcgg acgccgcaca cgtataacgc cgcgagcgtc acgggcacgg agtacctcgg
     1681 ccgcgcgatc ctcgtcgacc agtcgcccat cgggcgcacg ccgcgctcga accctgcgac
     1741 gtacaccggc gcgttcacgt atatccgcga cctgttcgcg tcgacgagcg aagcgcgtgc
     1801 gcgaggctgg aagtcgggac gcttctcgtt caacgtgaag ggcggccggt gcgaggcttg
     1861 ccaaggaaac ggcgaagtcg ccgtcgaaat gcacttcctc ccgacggtgt acgtgacgtg
     1921 cgacgtatgt gacggcaagc gcttcacgaa ggagacgcta gaagtccgct acaaagggaa
     1981 gaacatcgac gacgttctcc acatgaccat cgaggaggcg gacgcgttct tcatcgacgt
     2041 acccgctatc catgagcgcc tccagtccct tatcgacgtc ggactcgggt acctcgagct
     2101 cggccagagc gcaacgacgc tctccggcgg cgaggcgcag cgcgtgaaga tcgcatccga
     2161 actgtaccga ccgcatacgc agaagacgat ctaccttctc gatgaaccga cgatcggtct
     2221 tcactacgag gatgtccgca agctcatcga gatactcgac gagctcgtga agcgagggaa
     2281 cacggtcatg gtcatcgagc acaatctcga cttcatcaag gcgtgcgact acgtcatcga
     2341 catcggtccg gagggagggg agaagggcgg cgacgtcgtt gcgaagggga cgccggaaga
     2401 agtggcgaag aaccccaagt cgtacacggg gacatacctc aaaaagtcgc tctcccatgg
     2461 atagggcgaa gttcgcgcgg atgaaactgc cggatgcgcc gggtgtctac tttttcatag
     2521 gtccgcggaa ggagatactc tacatcggtc gggcgacatc ccttctccac cgcgtgaaga
     2581 gctacttcga cgatgacata gcctcgaagc ggagtcccgc gatcgagaag atggtgcgtg
     2641 agtcggttcg catagacgtc gaggagaccg attccgttct cgaggcgatc atcctcgagg
     2701 tgaatctcat cagaacgcac aaaccccgct acaatacgct ctcgaaggac gacaagagct
     2761 ataaccacgt cgccatcacc gacgaggagt tcccgcgcgt cctcgtcctc cgggggaagg
     2821 accttcgcga tgtgaagcac gaatggtcgt actcgcacgt ctatggtcca ttcccgagcg
     2881 ggaagcttct tcgcgacgcg ctccgcatca tacgacgcat cttcaagttc tacgacgtaa
     2941 agcggcccat ccgcgagaag atgagcaagg tcctgcgcgg cacgatcgat ttcaatcgcc
     3001 agataggact ctaccccgag agcacgtcgc ccgatgcgta tcaggagacc atacgacaca
     3061 ttcggctctt cttcgagggg cgcaagcgta cggtcatcgc ggaactgaag cgggatatga
     3121 cacgcttcgc gaagcaagaa cggttcgagg aagcgggcga ggcaaagcgg aagatactcg
     3181 cgctcgagca tattcaggac gtgtcgctcc ttcgtgacga cctgcgcgcg taccgcgacg
     3241 atcgaagcat gcgcattgaa gcgtacgaca tcgcgcacct cgcgggaacg catatggtcg
     3301 gcgtcttcac cgtcgtcgaa ggcgggaagc ccgacccgag ctcgtatcgc aaattcaacg
     3361 ttcgtgggtt caccgcgtcg aacgatcccg gcgctctccg agaggtcctc gaacgacgcc
     3421 tccgtcacga ggaatggcgg ataccggacc tcatcgtcgt cgacggaaac gccgtacaaa
     3481 agcgcgtcgc cgagcgtgcc gcgaaggcgg caggggtcgt cgtacccgtc gcggccgtcg
     3541 tgaaggacga acggcatcgt cctgagcgca ttctcggccc gcgtcatatc gtcgaggccc
     3601 gagcggagga gatactcctc gcgaacgccg aggcgcatcg attcgcgatc gcgttccatc
     3661 gcaggaaacg cggcaagctc cccgaataac gatatacgtt gcgaattcga cgttttgaag
     3721 gtatagtacc cccaccatgg caatcacgaa aaacgcgaag aaggcggtcc gctcccaggc
     3781 acggaagcgc gtctataacg tgcgcacgaa ggcgaagctc cacgacaccg tgaagtccct
     3841 caaggacgcg ctcgccaagg
//
LOCUS       DASTSP010000020        12623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1601994, whole genome shotgun sequence.
ACCESSION   DASTSP010000020 DASTSP010000000
VERSION     DASTSP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12623
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601994"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..749
                     /locus_tag="VFS75_00840"
     CDS             441..749
                     /locus_tag="VFS75_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677246.1"
                     /translation="MKILAAVSCILFSFAVHASPSKNSFEEERLRRNCCVSARPDIPV
                     ERPAFRIGQHDDWLHELVRKKRIRINAGNVIPPDDKVYFLRRGGKFMIEYRRRIHFLE
                     "
     gene            complement(839..1309)
                     /locus_tag="VFS75_00845"
     CDS             complement(839..1309)
                     /locus_tag="VFS75_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677247.1"
                     /translation="MKRRVHNGNRYGGMAQTARQGRKASPPKTVVVVREDALDRFNTR
                     YVKRPQKVTPPLESVSFILWERVSKSVRIRLEKIYEAFDAIRTRYSSFHVRLCENFLD
                     ALKRFASGAEELIAVVIAEGAYPGAGKFVQLRVANQTFAVPDSLVSGLRKNKKP"
     gene            complement(1435..1980)
                     /locus_tag="VFS75_00850"
     CDS             complement(1435..1980)
                     /locus_tag="VFS75_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677248.1"
                     /translation="MKELDELIVGYKRVGRRIVRGPNYAGTGGTIHKKPVETRDVAGD
                     DTEDDEVPSVPSAREKVKRKEKRAERMRAGKSGPVRKITLDVAEALKCAAKPRTANSD
                     GKLFEEFEIGIRHVGLIGTRKISFTVVSSRTMRHGTATVTYMRLARGTREWELPEHAF
                     RRRFESAIVAQHPKPKTMVYK"
     gene            complement(2045..2899)
                     /gene="cyoE"
                     /locus_tag="VFS75_00855"
     CDS             complement(2045..2899)
                     /gene="cyoE"
                     /locus_tag="VFS75_00855"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU4677249.1"
                     /translation="MMAGIYYRLAKPGIVYGNSFAAAAGFMLAAHGAPNPMLLLLTLV
                     GLGFVIGGACVFNNVIDRDIDVRMERTKGRALVTGQISPLNALVFGAVLSLIGFTFLA
                     VFANVLAFAAAAFGFFAYVVLYSLFAKRGTMYGTEIGSLSGAMPPVVGYAAASGTVDL
                     GAVLLFLILVFWQMPHFFAIALYRKNEYAAAGIPTVPNRKGVLFTKRVMLAYTALFLA
                     ALIALYHFGYVGKIFLALMSALGVAWVVMGIRGFRTPDDSRWARRMFRFSLILLVALS
                     MLIAFDAR"
     gene            complement(2896..3252)
                     /gene="cyoD"
                     /locus_tag="VFS75_00860"
     CDS             complement(2896..3252)
                     /gene="cyoD"
                     /locus_tag="VFS75_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEU4677250.1"
                     /translation="MTADSHNYILGYLLSVTLTLLAPVFLLVHEVTGHSLFAHTTLRV
                     AFLALALVQLVVQLVFFLHIRGEKRPRWRFMTLVSTVLIVVIVVGGSLWIMENLDARM
                     GSMSPDAMDAYMLDQE"
     gene            complement(3249..3839)
                     /gene="cyoC"
                     /locus_tag="VFS75_00865"
     CDS             complement(3249..3839)
                     /gene="cyoC"
                     /locus_tag="VFS75_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003174469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEU4677251.1"
                     /translation="MNIREEHMEERERASIASVGFWVYIMSDCLLFGSLFATFAVLEG
                     NTFAGPALGKHFSLPFVLVETMLLLTSSVTAGFGLLAARRGARRHVLAWLFATFLLGA
                     AFVALEIAEFRTLVLEGYGPSTSAALSSFFTLVGTHGLHVATGLLWMAVLMIYVVARG
                     ITGGALRRLTLFTLFWHFLDIVWIFIFTIVYLFNFI"
     gene            complement(3851..5833)
                     /gene="cyoB"
                     /locus_tag="VFS75_00870"
     CDS             complement(3851..5833)
                     /gene="cyoB"
                     /locus_tag="VFS75_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEU4677252.1"
                     /translation="MLGKLTIDALPHDPIVIGASIFMVVVGLIIVGLLYRYDKFGWLW
                     REWLTSLDPKRIGVMYIIVSFVMLFRGFSDAVMMRVQQALSVGASHGYLEPSHFQQIF
                     TAHGTIMIFFVAMGLMWGLFNVVVPLQIGARDVAFPFLNSLSFWLFVSGAVLMNLSLV
                     LGDFAKTGWLAYPPLSELAYSPDVGVDYWIWALQISGLGSLLSGVNFFTTILTMRCPG
                     MTLMRMPVFVWTTLCSTALVILAFPILTATLAMLFLDRFADFHFFTSTMGGNPMMYVN
                     LIWAWGHPEVYILVLPAFGVFSEVVPAFSQKRLFGYTSMVFATAGITFFSFVVWLHHF
                     FTMGAGANVNAFFGIMTMIIAIPTGVKVFNWLFTMFRGRIIFSSMMHWFLGFVATFTL
                     GGVTGVLMAVPAADFQLHNSLFLVAHFHNMIIGGVVFGYFAGMTYWFPKIFGFTLNER
                     LGKAAFWSWIIGFFLAFVPLYILGFMGATRRLDHYDASLGWQSLFIVAAIGVAVIGLG
                     VLFQVAQYVVSIRDRRKHRDVTGDPWNGRNLEWSTASPAPFYNFARIPRVYGRDAWWA
                     DKQSEELNAQGPYEDIELPKNSPAGLYIGVATLAIGFGLVWHIAALVLIGALVALGLL
                     IARTLDDDTERVIPAAEVERMDREFAKRRLDLHAAS"
     gene            complement(5837..6679)
                     /gene="cyoA"
                     /locus_tag="VFS75_00875"
     CDS             complement(5837..6679)
                     /gene="cyoA"
                     /locus_tag="VFS75_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEU4677253.1"
                     /translation="MLSQLFSYVGPGTFAVLDPRGPVGLDERNLMLTALVLMLGVLIP
                     VFMLTAAIAWRYRAGNTKARYTPNWEHNALDEFIWWAIPCAIIFVLALITWKSTHDLD
                     PYRPLRPGMESMPIQVVALDWKWLFIYPNEGIATVNFVEFPEDTAIDFEITADAPMNS
                     FWIPQLGGQIYAMPAMTTHLNLMASGTGSYFGTSANISGEGFSGMTFTAKSVSEEEFE
                     RWVESVRAGTSTLTREAYDALAKPSAYEPVATFSAVEDGLYNTIIGKYLAPPASSSGT
                     ARTH"
     gene            complement(6770..7198)
                     /locus_tag="VFS75_00880"
     CDS             complement(6770..7198)
                     /locus_tag="VFS75_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677254.1"
                     /translation="MKKEIRNVALTFLLVLAIAGRSAYVFAKENAMSDDAVSEPAVAT
                     VDGEAGSDPIAEAVLEPTDAPAKLEPTPAPARRAPASTQNVERPAAPADTETLAALQA
                     KLEAAKAQIEAVAAPKPAPQPTVVAKTPTVKHVVRRSRAS"
     gene            complement(7203..7373)
                     /locus_tag="VFS75_00885"
     CDS             complement(7203..7373)
                     /locus_tag="VFS75_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEU4677255.1"
                     /translation="MAQYVCLECGWIYDEAVGDPSRGIAPGTRWESLPDDFTCGECDV
                     KKSETHKWQRLE"
     gene            complement(7429..8358)
                     /locus_tag="VFS75_00890"
     CDS             complement(7429..8358)
                     /locus_tag="VFS75_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4677256.1"
                     /translation="MSTFFILLISSAFVYVLFTPPAPSGVVEEEVFIEGDETIEVGPY
                     EYREHIEAVLVVADIFVLLIASIFSYAFARRTLRPIEEMRAREERFMADVAHELRTPL
                     AVMRSGNDTVLRKPRSPQEYERFIRESNDEVKRLAGLTEGLLTLLTNERERAEACADV
                     DFGALARREVARFASYAAERSVSLSADRTEEVIVHADAGSLVQLVQNLLKNAVDYNRP
                     GGTVTVHIVRDGLDAVLSVRDTGVGIAETAQRHVFDRFYKVDEARSDVTRGAGLGLAI
                     ARDIARRFGGDIALESAPGAGSTFTVRLPLAHS"
     gene            complement(8399..9067)
                     /locus_tag="VFS75_00895"
     CDS             complement(8399..9067)
                     /locus_tag="VFS75_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4677257.1"
                     /translation="MKLLVVEDNEKLAKYVAQTFEEEAYAVDVVHDGETGERRIESGS
                     YDAVILDIMLPEKDGIAVCKSVRAAGITVPILMLTARGEVEDRVLGLDSGADDYLLKP
                     FDMNELLARVRALLRRPQSHLAEKLTVQDVVLDTATRSVAVGGKERALTLKEYAVLEY
                     LMRNAGTVITREQLLDHCWDFAYSAFSNITDVYIKQLRQKLGGDHDRYIKTIRNVGYT
                     FKTE"
     gene            9127..10014
                     /locus_tag="VFS75_00900"
     CDS             9127..10014
                     /locus_tag="VFS75_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU4677258.1"
                     /translation="MNEYAYARRYMGTDVSLSFVVPTEALALTMAEDLFRSIARYESL
                     FSRFLPESELSRLNRSKAARVSREFEAVLRRSTDLVHRTENAFNPLVQVARLGYVRSF
                     AEGPRGATVDATPYDTDITAVGVDTDGTVRLRGAQNLDFGGILKGYLASLLVRHVQGE
                     YPACTGAIVNLGGDLATFGRDADGKPFVFEVYNPVTGHDLPLTVENSALATSGTYARS
                     WETKQGRRHHIVDSKTRENPRTNIVSATVVHEDGAVAEAFTKVFISRPFDEAMRIVGK
                     EPLGFLLILVNGSVVSSLV"
     gene            10011..10640
                     /locus_tag="VFS75_00905"
     CDS             10011..10640
                     /locus_tag="VFS75_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677259.1"
                     /translation="MKTYFAFIRITQEALLAVSVAMLATLPTVLAFNPDLFTDASMAF
                     LYAVAHTALFFVMMIRPLSDLFPSVPYLRPLVILRKGAGVLSASVIVSFLLAKVIVDP
                     SGYLSSIGTGAYWSLHNLALFAHLGDLSAVVLLATSNALSKRVLGKNWKRVQRLSYVY
                     FYASALYVTLMLHTTYVLVYVVFVTLATVFALLKNRGLLGAESYTPKQA"
     gene            10637..11332
                     /locus_tag="VFS75_00910"
     CDS             10637..11332
                     /locus_tag="VFS75_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4677260.1"
                     /translation="MIRKVKGIIRNVNDLSPTAKELVIDLEEPLPFVAGNFVNLFFPK
                     DGETVRRAFSLSSSDADSRTIALSVRRSRGMAGLTPYFWEHDVAGTPVELMGPLGENT
                     ADKLTSKKAFLFAFGVGAGVIRSVADHLVKRASPPELRIMTGSRDDGDIIHKDYFDEL
                     AAMGKAHVRYVVSTPGTSPFLSGYIQDHVSDFDFNDADVYMCGQDAACTELQTKVLST
                     APKNCRLFVESFG"
     gene            complement(11465..11539)
                     /locus_tag="VFS75_00915"
     tRNA            complement(11465..11539)
                     /locus_tag="VFS75_00915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11504..11506),aa:Arg,seq:tct)
     gene            complement(11661..12029)
                     /locus_tag="VFS75_00920"
     CDS             complement(11661..12029)
                     /locus_tag="VFS75_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677261.1"
                     /translation="MKFSFHKRGKKKPSFHLWKDGTSTYMNPSRDWTIGLVIASVGFV
                     VGATLIGFDFYSQLRSEGADVPPQTSQMLYDQNGVTEYARIYSRREDAFNALRAGGTP
                     APVLTPVATTTATTTPLAGE"
     gene            complement(12026..12538)
                     /locus_tag="VFS75_00925"
     CDS             complement(12026..12538)
                     /locus_tag="VFS75_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677262.1"
                     /translation="MIAVFGSAFFVVSLAALFIFSHLVSLKKEEYQRETLAAAEAEAH
                     NAALSQLTRTLDETKEDRDSVNGRILKDEDVIGFLALIETLGKEQGVSLSTSALTVEP
                     IDATFERLVMSVAVSGPYQGVIRTLEVLENLPYQSSVLHLQLSRGEAEDGSQVWKSTY
                     DLTITKFKKR"
BASE COUNT         2541 a         3826 c         3862 g         2394 t
ORIGIN      
        1 ctcgtttaca ccgagaaggt cgggggttca agtccctcac cgcccacaaa gtgaaaatcc
       61 cagaggtcct tctctgggat ttttgctttt gggccatggg acttgaagac ctcgcgagag
      121 ggttttggag cttgcgaaca aaaccctcag caaggtgtac tgaacatgta atgttcaagt
      181 ccctcaccgc ccacaaaaaa gaagcgcccg ggggcgcttc aatcactcaa ttctgttgac
      241 cggctagtcc gcgtgccacc gggcgctctc atcctgcctc tcccgtagct tcactgggag
      301 gttccgcgaa taggctcttg gggctctgta tataagtgta cgcgatggag gaatacgcgc
      361 aaaagtcgac ggtgcatagg gtactggaaa tcgccgctag ggcctgtact ataggagagg
      421 tccacccacg aaaggttcgc atgaagatcc tcgcagcggt ctcctgcatc ctgttctcgt
      481 tcgcggtcca cgcaagcccc tcgaagaact ccttcgaaga ggagaggctc cggcggaact
      541 gttgcgtctc ggcacggccc gacatacccg tcgagcggcc ggcgttccgc atcggacagc
      601 acgacgattg gcttcacgag ctcgtgcgga agaagcgcat tcggatcaac gcggggaacg
      661 tcatcccccc ggacgacaag gtgtactttc tccgtcgggg cggcaagttc atgatcgagt
      721 accgccgccg catccatttc ctcgagtaac gctcggggac gcccacgcga caaacgtggg
      781 cgtttttatt ccatagaaaa accctcgcac ggaacgaggg tcgagcgact ccggggagtc
      841 aaggtttttt attcttccga agcccggaga cgagcgaatc cggaactgcg aacgtctggt
      901 tcgcgacgcg gagctgaacg aacttgcccg cgccggggta cgcaccttcc gcgatgacga
      961 ccgcaatgag ctcttcggcg cccgaagcga accgcttgag ggcgtcgagg aagttctcgc
     1021 agagccgcac gtggaacgat gagtaccgcg tacgtatcgc atcgaacgcc tcgtagatct
     1081 tctcgaggcg gatgcgaacg cttttcgaga cccgctccca gaggatgaac gacacgctct
     1141 cgaggggtgg cgtcaccttc tgcggccgct tcacgtaccg cgtattgaag cggtcgagag
     1201 cgtcctcccg cacgacgacc accgtcttcg gcggcgacgc cttccgaccc tgccttgcgg
     1261 tctgcgccat tccgccgtag cggttcccgt tatgcaccct ccgtttcaaa ccacgaactg
     1321 catgagtcac gtgaatctcc cttgttgtgg acgtgcgcat cacaaaatga tgcatcgaca
     1381 tagtacacct ttttcggaga atttaaagca aaaaaaatga gcccccgtga gagctcattt
     1441 gtacaccatc gtcttcggct tcggatgttg ggccacgatg gccgactcga agcgacggcg
     1501 aaacgcgtgt tccgggagtt cccactcccg cgttccgcga gcgagccgca tgtacgtcac
     1561 cgtcgccgta ccgtggcgca tcgtgcggct cgagacgacc gtgaagctta tcttccttgt
     1621 gccgatcaac cccacgtgcc ggatgccgat ctcgaactcc tcgaagagtt ttccatcgga
     1681 gttagccgtc cgcggtttcg ctgcgcattt gagagcctcc gccacgtcga gggtaatctt
     1741 ccgaaccgga ccgctcttcc ctgcacgcat gcgctcggcg cgcttctcct ttcgcttcac
     1801 cttctcgcgg gcggacggaa cgctcgggac ctcatcgtcc tcggtatcgt cgccggcgac
     1861 gtcccgcgtt tcgacgggct tcttgtggat cgtcccgccg gtccccgcgt agttcggacc
     1921 gcggacgatc ctccgcccga cccgcttgta gccgacgatg agctcgtcaa gttccttcat
     1981 cgatacctcc tttcttggcc gaccactata gtacactgcg ccgtcgtttt gaaaagggcc
     2041 gatactaccg cgcgtcgaat gcgatgagca tcgagagagc gacgaggaga atgagcgaga
     2101 agcggaacat gcggcgcgcc caccgagaat catcgggcgt gcggaaaccg cggatgccca
     2161 tgacgaccca cgcgacgcca agcgcgctca tgagcgcgag gaatatctta ccgacgtacc
     2221 cgaagtggta gagggcgatg agcgcggcga ggaagagcgc cgtgtacgcg agcatgacgc
     2281 gcttcgtgaa gaggacgcct ttccggttgg gcacggtcgg gatgccggcc gcggcatatt
     2341 cgttcttccg gtagagcgcg atcgcgaaga agtgcggcat ttgccagaag acgaggatga
     2401 ggaagaggag gaccgctccg aggtcgacgg tgccggaggc tgctgcgtac ccgacgaccg
     2461 gcggcatggc gcccgagagg gacccgatct ccgtcccgta catggtcccg cgtttcgcga
     2521 agaggctgta gaggacgacg tacgcgaaga acccgaaggc cgcggcggcg aacgcgagca
     2581 cgttcgcgaa caccgcgagg aacgtgaagc cgataagcga aagcaccgct ccgaagacga
     2641 gcgcgtttag cggcgagatc tgccccgtga cgagcgctct tcccttcgtc cgctccatgc
     2701 gaacgtcgat atccctatcg atgacattgt tgaagacgca cgcgccgccg atgacgaacc
     2761 cgagaccgac gagcgtgaga aggagaagca tgggattcgg tgcaccgtgt gccgcgagca
     2821 taaagcccgc agcggcggcg aacgagtttc cgtagacgat gcccggtttc gcgagcctgt
     2881 agtagatccc tgccatcatt cttggtcgag catatacgcg tccatcgcgt cgggcgacat
     2941 cgaccccatg cgcgcatcga ggttctccat gatccagagc gacccgccga ccacgatgac
     3001 gacgatgagc accgtcgaca cgagcgtcat aaatctccag cgcggccgtt tctcgccgcg
     3061 gatatggagg aagaagacga gctggacgac gagctggacg agcgcgagcg cgaggaacgc
     3121 aacgcggagc gtcgtgtggg cgaagagcga gtggccggtc acctcgtgaa cgagaaggaa
     3181 gaccggcgcg agaagcgtga gcgtcaccga gaggaggtag ccgagtatgt agttgtgaga
     3241 gtcggcggtc atatgaaatt gaaaagatac acgatggtaa agatgaatat ccaaacgatg
     3301 tcgaggaagt gccagaagag cgtgaagagc gtgagccgcc ggagcgcgcc tcccgtgata
     3361 ccccgggcaa cgacgtagat catgaggacg gccatccaga ggaggcccgt cgcgacgtgg
     3421 agtccatgcg tgccgacgag cgtgaagaac gaggagaggg cggcgctcgt gctcggccca
     3481 tacccttcaa gaacgagcgt ccggaactcg gctatttcga gagcaacgaa cgccgcgccg
     3541 agaaggaacg tcgcgaagag ccacgcgagc acgtgacgcc gcgctccccg ccgtgcggcg
     3601 agaaggccga agcccgccgt aacgctcgat gtgaggagga gcatcgtctc gacgaggacg
     3661 aaagggagcg agaaatgctt cccgagcgcc ggcccagcga acgtattccc ctcgaggacg
     3721 gcgaacgtcg cgaagagact cccgaagagg agacaatcgc tcatgatgta gacccaaaag
     3781 cctaccgacg cgatgctcgc ccgttcgcgt tcctccatgt gctcttcacg tatgttcatg
     3841 gtgtcgtgat tcatgatgcg gcgtgtaggt cgaggcgccg tttcgcgaac tcgcggtcca
     3901 tgcgttcgac ctcggcggcg ggaatgacgc gctccgtgtc gtcgtcgagc gtgcgggcga
     3961 tgagaaggcc gagcgcgacg agcgccccga tgagtacgag cgcggcgata tgccacacga
     4021 gaccaaagcc gatggcgagg gtcgccacgc cgatgtagag gcccgcagga ctgttcttcg
     4081 ggagctcgat gtcctcgtac ggcccctgcg cgttcagctc ctcgctctgt ttatcggccc
     4141 accatgcgtc tcgaccgtag acgcgcggta tacgcgcgaa gttgtagaac ggcgcgggcg
     4201 acgcggtcga ccactcgaga ttccgaccgt tccatgggtc acccgtcacg tcccggtgct
     4261 ttcgcctgtc gcgtatactc acgacgtact gcgcgacctg gaagaggacg ccgagcccga
     4321 tgaccgcgac gccgatcgcc gcgacgatga agagcgactg ccatcccaag gacgcgtcgt
     4381 agtggtcgag gcgtcgcgtc gcgcccatga atccgagaat gtagagcggc acgaacgcga
     4441 ggaagaagcc gatgatccag gaccaaaatg ccgccttccc gagccgctca ttgagcgtga
     4501 agccgaatat cttcgggaac cagtacgtca ttcccgcgaa atacccgaat acgacgccgc
     4561 cgatgatcat gttatggaag tgcgcgacga gaaagaggct gttgtggagc tggaagtcgg
     4621 cggcgggtac cgccatgagg acgcccgtca cgccgcctaa ggtgaacgtc gcgacgaatc
     4681 cgaggaacca atgcatcata gaggagaata tgatgcggcc gcggaacatc gtgaagagcc
     4741 agttgaacac cttcaccccc gtcggtatcg cgatgatcat cgtcatgatt ccgaagaacg
     4801 cgttcacgtt cgcaccggct cccatcgtga agaagtggtg gagccacacg acgaacgaaa
     4861 aaaaggttat gccggccgtc gcgaacacca tcgacgtgta cccgaagagt cgtttttgcg
     4921 agaacgcggg aacgacctcg ctgaagacgc cgaacgcggg aaggacgaga atgtacacct
     4981 ccggatgacc ccacgcccaa atgaggttca cgtacatcat cggattaccg cccatcgttg
     5041 acgtgaagaa gtggaagtct gcgaatcgat cgaggaagag catcgcaagc gtcgcggtga
     5101 gtatgggaaa cgcgagaatg acgagcgcgg tcgaacagag cgtcgtccag acgaacacgg
     5161 gcatgcgcat gagggtcatc cccggacagc gcatcgtgag aatggtcgtg aagaagttca
     5221 ctcccgagag gagactcccg agtcccgata tctggagcgc ccatatccaa taatcgacgc
     5281 cgacatccgg actgtacgcg agctcggaga ggggcgggta cgcgagccac cccgtcttcg
     5341 cgaagtcgcc gagaacgagc gagagattca tgagaacggc acccgagacg aagagccaga
     5401 acgagagcga gttcaagaac gggaacgcga cgtcgcgggc accgatctgc agcgggacga
     5461 cgacgttgaa gagcccccac atgagtccca tagcgacgaa gaagatcatg atggtcccgt
     5521 gcgccgtgaa tatctgttgg aagtggctcg gctcgaggta cccgtgcgac gcgccgaccg
     5581 aaagcgcctg ttggacgcgc atcatgaccg catcgctgaa tccgcggaac agcatgacga
     5641 acgatacgat gatgtacatg acgccgatgc gtttggggtc aagggacgtg agccactcgc
     5701 gccagagcca gccgaactta tcgtaccggt agagaaggcc gacgatgatg aggcctacga
     5761 ccaccatgaa gatggacgcg ccgatcacga tcgggtcatg cgggagcgca tcgatggtga
     5821 gcttgcctaa catgtttcaa tgggttcgcg ccgtgcccga cgatgacgca ggcggcgcga
     5881 ggtacttccc tatgatcgta ttatacagac cgtcttcgac ggcggagaag gtcgcgaccg
     5941 gttcgtacgc actcggcttc gcgagcgcgt cgtacgcctc acgcgtgagc gtcgaggttc
     6001 ccgctcgcac ggactcgacc caccgttcga actcctcctc ggacacgctc tttgccgtaa
     6061 acgtcattcc cgagaacccc tcgccgctta tgttcgcgga ggttccgaaa tagctccccg
     6121 tgccgctcgc catgaggttg agatgcgtcg tcatcgccgg catcgcgtat atctgtccgc
     6181 cgagctgcgg tatccagaac gagttcatcg gggcatcggc ggttatctcg aaatcgatgg
     6241 cggtgtcctc ggggaattcg acgaaattca ccgtcgcgat gccctcattg gggtagatga
     6301 agagccactt ccaatcgagg gcgacgacct ggatgggcat cgattccatg cccggtcgta
     6361 gcggccgata cgggtcgagg tcgtgcgtgc tcttccacgt gatgagcgcg aggacgaaga
     6421 tgatggcgca cggtatggcc caccagatga actcgtcgag cgcgttgtgc tcccaattcg
     6481 gcgtgtagcg cgccttcgta ttccccgcgc ggtaccgcca cgcgatcgcc gcggtgagca
     6541 tgaagacggg gatgagaacg ccgagcatga ggacgagcgc cgtgagcatg aggtttcgtt
     6601 cgtctaaccc gacgggccca cgaggatcga gaacggcgaa ggttccggga cccacatagg
     6661 aaaagagctg cgagagcatg tctgcattat aacagagccc tctcacggca tgcggtcacc
     6721 gcgcacaaga aaacgacccg acgcgggtcg agtcgtttcg tacgcggtat tagctcgcgc
     6781 gtgaccggcg aacgacgtgt ttcaccgtcg gcgtcttcgc gacgaccgtc ggctgtggcg
     6841 cgggtttcgg cgcggcgacg gcctctatct gcgctttcgc ggcttcgagc ttcgcctgaa
     6901 gcgcggcaag cgtctcggta tccgcgggcg ccgccggcct ctcgacgttc tgcgtcgacg
     6961 cgggtgctct ccgcgccgga gcgggcgtcg gttcgagctt cgccggcgca tcggtcggct
     7021 caagaaccgc ctcagcgata gggtcgctcc ccgcctctcc atccacggtg gcaacggccg
     7081 gttcggatac cgcgtcgtcg ctcatcgcgt tctccttcgc gaagacgtac gcgcttcgcc
     7141 ccgcgatcgc gaggacgagg aggaacgtca atgctacgtt tcgtatttcc tttttcatgg
     7201 tgttactcga ggcgctgcca cttatgcgtc tcgctcttct tcacatcgca ctcgccgcac
     7261 gtgaagtcgt cggggagcga ttcccagcgt gtgcccgggg ctataccccg agacggatcg
     7321 ccgaccgcct cgtcgtagat ccacccgcac tcgagacaga cgtattgcgc catagtttag
     7381 ccgttatgcc gataatgcgc ataacggtac cagccgaaga tgagtggctc atgaatgagc
     7441 gagcgggagt cgaaccgtga acgtcgaacc ggctcccggc gcgctctcga gcgcgatgtc
     7501 cccgccgaaa cgccgcgcga tatcccgcgc tatcgcgaga ccgagtccgg cgccgcgcgt
     7561 cacgtcgctt cgcgcctcgt ccactttgta gaagcggtcg aacacgtgac gctgtgcggt
     7621 ctcggcgatg ccgacgcccg tatcgcgtac ggagagaacg gcgtcgagcc cgtctctcac
     7681 gatatggacg gtgacggtgc cacccgggcg gttgtagtcg acggcgttct tgaggagatt
     7741 ctggacgagt tgaacgagac tccccgcgtc ggcgtgaacg atgacctcct cggtcctatc
     7801 cgcggaaagg gacacggacc gttccgccgc gtacgaagcg aaacgagcca cttcgcggcg
     7861 cgcgagcgcg ccgaagtcga cgtccgcgca cgcctccgca cgctcgcgtt cgttcgtaag
     7921 gagcgtaagg agaccctcgg taaggccggc gagccgcttc acctcgtcgt tcgactcgcg
     7981 gatgaaccgc tcgtactctt ggggagagcg aggcttcctc aaaacggtat cgtttcccga
     8041 gcgcataacg gcgagcggcg tccgaagttc gtgcgcgacg tccgccatga accgctcttc
     8101 ccgtgcgcgc atctcctcga tgggacggag cgtccggcgc gcgaacgcgt acgagaagat
     8161 gctcgctatg aggaggacga agatatcggc gacgacgagg accgcttcga tatgttcgcg
     8221 atactcgtac ggtccgacct cgatcgtctc atctccctcg atgaacacct cctcttcgac
     8281 gacaccgctc ggcgcgggag gagtgaagag aacgtacacg aacgcgctcg agatgaggag
     8341 gatgaagaat gtgctcacga cgtagtagag cgtgagccgt aacgtcgctc gtctgaactc
     8401 attccgtctt gaacgtgtat ccgacgttgc ggatggtttt gatgtatcgg tcatggtcgc
     8461 cgccgagctt ctgacgaagc tgctttatgt atacgtccgt tatattacta aacgcgctgt
     8521 acgcaaagtc ccagcaatgg tcgaggagct gttctcgcgt gatgaccgtg cccgcgttcc
     8581 gcatgaggta ctcgaggacg gcgtactcct tgagcgtgag cgcgcgctcc ttccccccta
     8641 cggcgaccga acgcgtcgcg gtgtcgagga cgacgtcctg aacggtgagc ttctcggcga
     8701 ggtgggactg cggccggcgg agaagcgcac gcacgcgagc gaggagctcg ttcatgtcga
     8761 agggtttgag gagatagtcg tccgccccgc tgtcgaggcc gaggacgcga tcttcgacct
     8821 cgccgcgcgc ggtgagcatg agtatgggta ccgtgatacc cgccgcgcgg accgacttgc
     8881 atacggcgat accgtccttc tccggaagca tgatgtcgag gatgacagcg tcgtacgagc
     8941 cgctctcgat gcgccgctcg ccggtctcgc cgtcatgaac gacgtcgacc gcgtacgcct
     9001 cttcctcgaa ggtttgcgcg acgtacttcg cgagtttctc gttatcctcg acgacgagta
     9061 gcttcatgtg ggccatgata cacccattca cgatgcattc atattgaccc ggtaccgtgt
     9121 cgcaatatga acgaatacgc gtacgcgaga cggtacatgg gtacggacgt gagcctctcg
     9181 ttcgtcgtgc ccacggaggc gctcgcgctc accatggcgg aggacctttt taggagtatc
     9241 gcccggtatg aatcgctctt ctcacgtttt ctcccggaga gcgaactctc gcggctcaat
     9301 cgctcgaaag cagcccgcgt gtcgagagag ttcgaggcgg tcctgcggcg aagcactgac
     9361 ctcgtccatc ggaccgagaa cgcgttcaat ccgctcgtcc aggtcgcgcg tctcgggtac
     9421 gtccgaagct ttgcggaggg gccgcgcggc gcgaccgtgg acgcaacgcc gtacgatacg
     9481 gacatcaccg cggtcggcgt cgataccgac ggaaccgtgc gccttcgcgg ggcgcaaaac
     9541 ctcgacttcg gcggcatctt gaaagggtac ctcgcgtcgc tcctcgttcg acacgtacaa
     9601 ggcgaatacc ccgcgtgcac gggtgccatc gtgaacttag gcggcgacct cgcgaccttc
     9661 ggtcgcgatg cggacggcaa accgttcgtc ttcgaagtct ataacccggt gaccggccac
     9721 gatctgccgc tcacggtcga gaactctgcc cttgcgacga gcggtacgta cgcgcggtcg
     9781 tgggaaacga agcagggtcg ccgacaccat atcgtcgact cgaagacgcg cgagaatcca
     9841 cggacgaaca tcgtctcggc gaccgtcgtc cacgaagacg gtgcggtcgc cgaggccttc
     9901 acgaaggtat tcatcagtag acctttcgac gaggcgatgc gaatcgtcgg gaaagagccg
     9961 ctcggtttcc ttctcatcct cgttaacggc tcggtcgtaa gcagcctcgt atgaaaacct
    10021 atttcgcgtt cataagaata acgcaggaag cgctcctcgc cgtctcggtc gcgatgctcg
    10081 cgacgttgcc aaccgtactc gcgttcaatc ccgatctctt tacggatgcc tcgatggcgt
    10141 ttttgtacgc agtcgcgcac accgcgctct tcttcgtcat gatgatacgt ccgctttcgg
    10201 acctgttccc gagcgtaccg tacctccggc cgctcgtcat cctacggaag ggtgctggcg
    10261 tactttccgc ctccgtcatc gtatcgtttt tgctcgcgaa ggtcatcgtc gacccaagtg
    10321 ggtacctttc ctcgataggg accggggcgt actggtcgct ccataatctc gcgctcttcg
    10381 cgcaccttgg cgacctctcc gccgtagtgc tccttgcgac ctcgaacgcg ctctcgaagc
    10441 gggttctcgg caagaactgg aagcgcgttc agcgtctctc gtacgtgtac ttctacgcga
    10501 gcgcgctcta cgtgacgctc atgctccaca cgacgtacgt actcgtctac gtcgtcttcg
    10561 ttacgctcgc gacggtcttc gccctcctca agaaccgcgg gctcttgggt gccgagtcgt
    10621 ataccccgaa acaggcatga tacgaaaagt gaaaggcatc atccggaacg tgaacgacct
    10681 ctcgccgacc gcgaaggagc tcgtcatcga ccttgaagag ccactgccct tcgtcgcggg
    10741 gaacttcgtc aatctcttct ttccgaagga cggagaaacg gtacggcgcg cgttctcgct
    10801 ctcgtcgagc gacgccgact ctcggaccat cgcgctctcg gttcggcgct cgagaggaat
    10861 ggcgggactc actccgtatt tctgggagca tgacgtcgcc gggaccccgg tcgagctcat
    10921 gggtcccttg ggcgagaata ccgcggacaa gctcacgtcg aagaaggcgt ttctcttcgc
    10981 cttcggcgtc ggagccggcg tcattcgttc ggtcgccgat cacctcgtga agcgcgcgtc
    11041 gccgcccgag ctccgtatca tgacgggaag cagggacgac ggcgacatca ttcacaaaga
    11101 ctacttcgac gagctcgcgg ccatgggtaa agcgcacgtt cgctatgtcg tatcgacgcc
    11161 cggtacgtca ccgttcctct ccggatacat acaagaccac gttagtgact tcgatttcaa
    11221 cgatgctgac gtctacatgt gcgggcaaga tgcggcctgt acggaactac agacgaaggt
    11281 ccttagtact gctccgaaga attgccgctt gttcgtggag agtttcgggt aagaggaagg
    11341 gatcgaacct tacaggttca gtatccgttc aggatatttt ttcaaaatat ctctgaacgg
    11401 cttcgattcc tcccccgaac gccccgccac tagaaaaggc tggcgaactg ccaacctttt
    11461 ccagtggtgc ccccgggagg aatcgaacct ccatcaagag cttagaagtc tcctgttcta
    11521 tccattgaac tacggggacg ggcactcctt ctatatatac tatcaatctc ctcgtgaaca
    11581 tgccccgcat gtatcactcc attccctacg gggacgggca ctctttcctt ctctcgctca
    11641 tggcgagtgt actcgctctc ctactccccc gcaagcggcg tggtcgtcgc ggtcgtcgtc
    11701 gcgacgggcg tcaaaacggg cgcgggcgtg ccaccagccc gaagggcgtt aaaggcgtcc
    11761 tcacgtctgg aatatattcg tgcgtactcc gtgacgccgt tttgatcgta gagcatttgg
    11821 ctcgtctgtg ggggtacgtc cgcaccctcg gagcgcagtt gcgagtagaa atcgaagccg
    11881 atgagcgtcg ctccgacgac gaacccgacc gaggcgatga cgagcccgat ggtccagtca
    11941 cgagacgggt tcatatacgt cgacgttccg tctttccaaa gatggaacga cggtttcttc
    12001 tttccgcgct tgtgaaatga gaacttcatc gtttcttgaa cttagtgatg gtgaggtcgt
    12061 aggtgctctt ccaaacctgc gaaccgtcct ccgcctcgcc tctcgagagc tgaaggtgga
    12121 ggaccgaaga ttgatacgga aggttctcga gcacttcgag cgtccggatg accccctggt
    12181 acggaccgga cacggcgacg ctcatgacga gacgctcgaa cgtcgcgtcg atcggctcga
    12241 cggtgagcgc gctcgtcgag agcgagacac cctgctcctt ccccaaggtc tcgatgagcg
    12301 cgaggaaccc gatgacgtcc tcatccttga ggatgcggcc atttacggag tcacggtcct
    12361 ctttcgtctc atcgagcgtc ctcgtgagct gtgagagggc cgcgttgtgc gcctccgcct
    12421 cggcggcggc gagcgtttcc ctctggtact cttccttctt gagagagacg aggtgggaga
    12481 agatgaagag ggccgcgagc gacacgacga aaaacgcgct cccgaaaacc gctatcatgt
    12541 tcgtactctt gtgggtcgtc ggtgtcattt tgtcttagcg agggttaccg taacggtgaa
    12601 cgggaggtcc gcgtcgcgcg cga
//
LOCUS       DASTSP010000021         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_711192, whole genome shotgun sequence.
ACCESSION   DASTSP010000021 DASTSP010000000
VERSION     DASTSP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711192"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFS75_00930"
     CDS             complement(<1..995)
                     /locus_tag="VFS75_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU4677263.1"
                     /translation="MTKRSAAKKDYAKMALKLHKKLRGKLAVVSKGKLRSRDDWSTMY
                     TPGVGAVAGHLAKHEREARDYTLKGNTVAVVSDGSAVLGLGNLGPLGALPVMEGKCAI
                     LKEMAGVDAFPIVLSTQDTEEIIKTVKHISPTFGAINLEDISAPRCFEIEERLKAELP
                     IPVMHDDQHGTAIIVLAGLMNAAKVVGKRLSSLRVVIVGAGAAGRAIALLLVKAGIED
                     VIVSDSAGVIYQGRKGMAPYKCALAKVTNPRGVRGLLDAAVAGADAIVGVSGPGTISR
                     AHVESMAKDAIVFALANPIPEIYPEDATAAGAAVVASGRSDYPNQINNSLVFPGI"
     gene            1356..>3447
                     /gene="recG"
                     /locus_tag="VFS75_00935"
     CDS             1356..>3447
                     /gene="recG"
                     /locus_tag="VFS75_00935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU4677264.1"
                     /translation="MRPTDLLSDHFRIDKEHEKALKRLGLATVRDLLYHLPQRYEDIS
                     SVQSVEGLRKGEDAVVYGAVGGLKTRKAWKSRMPIAEGWVEDGSGRMKVMWFNQPYLA
                     KMLTDGTYVKLAGKVSGTEGKLYLANPEIEKLDSLPIDRHDSIFKNTGNLDETIYPVY
                     RETKGVTSKWFYHTVVKCFEKGVLDTLKDPIPEEILARYNLPTLTTALQWIHTPKKAE
                     HAKSARKRFAFEEVFYIQAQKAKERASVEAAETYRFKTDPAHILEFTKRFPFDLTPSQ
                     IKALADITADFDRRFPMSRLLEGDVGSGKTAVAATAAYVAATSRPSSNVSKNMGGLEY
                     GNLQVAYMAPTEILAKQHFESFIEYFKHLPIQIGLITGSGCMKFPSKVNAERATAISK
                     AQLLRYVKSGEIPIVIGTHALMYKSVEFRHLALVIIDEQHRFGTAQRKALAKKDARLP
                     HLLSMTATPIPRTLALTVYGDLDITLLSEMPKGRKPVITRLVAKGREKEMYAHVKEEL
                     DSGRQAYVICPRIDEADPEKETAIIAKSVVEEAKRLKKDVFPDRTIAVLHSKMKPAEK
                     DKVMAAFAKHDIDILVATSVVEVGVNVPNATNIIIEGAERFGLAQLHQLRGRVIRGTH
                     QPYCFVVTDSKSERTRERLKALTTARNGFELAEFDLAQRGSGELYGAKQSGLTDLGME
                     ALKNLKMVEAARTEA"
BASE COUNT          699 a         1123 c         1047 g          578 t
ORIGIN      
        1 aagatgcccg ggaagacgag cgagttgttt atctggttcg ggtagtccga gcggccgctc
       61 gcgacgacgg cggcgcccgc cgcggtcgcg tcctcgggat atatctcggg tatcgggttc
      121 gcgagcgcga agacgatcgc gtccttcgcc atcgactcga cgtgtgcgcg cgaaatggtt
      181 cccgggccgg atacgccgac gatcgcgtcc gctcccgcga ccgccgcatc gagaaggccg
      241 cggacgccgc gcgggttcgt gaccttcgcg agtgcgcact tatagggcgc catgcccttc
      301 cgtccctggt agatgacgcc ggcgctgtcg gaaacgatga cgtcctcgat accggccttg
      361 acgagaagga gcgcgatcgc gcgtcccgcc gcaccggcgc cgacgatgac gacgcgaagc
      421 gaggagaggc gtttcccgac gaccttcgcc gcgttcatga ggcccgcgag gacgatgatc
      481 gccgtgccgt gctggtcgtc gtgcatgacc ggtatgggta gctctgcctt aagccgctcc
      541 tcgatctcga agcagcgcgg cgcgctgatg tcctcgaggt tgatcgcgcc gaaggtcggc
      601 gagatgtgct tcaccgtttt gattatctcc tcggtgtcct gggtcgagag gacgatggga
      661 aacgcgtcga cgcccgccat ctccttgagt atcgcgcact tcccctccat gacgggaagg
      721 gcacccaagg gcccgaggtt cccaagtccg aggaccgcgg aaccgtccga gacgacggcg
      781 acggtgtttc ccttcagcgt gtagtctcgc gcctcgcgct cgtgcttcgc gaggtgcccc
      841 gcgaccgcgc cgacgcccgg cgtgtacatc gtgctccaat catcgcgact ccggagtttc
      901 cccttcgaga cgaccgcgag tttcccacgc agtttcttgt ggagctttag cgccatcttc
      961 gcgtagtcct tcttcgccgc tgatcgtttc gtcatgtacg tatatatgag ggatagtctc
     1021 aaagtatact cctccgagca cgaaataaaa aaaccttcag cgtggagctg aaggggatgg
     1081 aagaacgggg agagaggaag gaagaagaac gaatccaggg cgaatgccaa ggatggaaga
     1141 acgatgctcg gtcactgcga ggaggccgaa ttgcgggtcg agccctgtta catttcgtca
     1201 aggactacat cggggagcgt aggctccccg cccgcggcgc ccgcaccctt taacaagtgc
     1261 gggcgccgca atctcctctc actatacgcc tcccccttcc cccgtcaaga gtcaaaaaac
     1321 ctcgtcgttt cgggcacatt tgctactata gtcacatgcg accgacagac ctcttaagcg
     1381 accacttccg tatcgacaaa gagcacgaga aggcgcttaa gcgcctcggc ctcgcgaccg
     1441 ttcgcgacct cctttaccac ctcccccaac gctacgagga catctcctcc gttcaatcgg
     1501 tcgaggggct tcgaaagggc gaggatgccg tcgtctacgg agcggtcgga ggcctcaaaa
     1561 cgcggaaggc gtggaagagc cgcatgccta tcgccgaggg ctgggtcgag gacggttcgg
     1621 gccgcatgaa ggtcatgtgg ttcaatcagc cgtacctcgc gaagatgctc acggacggaa
     1681 cgtacgtgaa gctcgcgggc aaggtctcgg gaacggaggg aaaactctac ctcgcgaacc
     1741 cggagatcga gaagctcgat tccctcccga tcgaccgtca cgattccatt ttcaagaata
     1801 cggggaacct cgacgagacc atctacccgg tctaccgcga gacgaagggc gtcacctcga
     1861 agtggttcta ccacaccgtc gtgaagtgct tcgagaaggg cgtgctcgac acgctcaagg
     1921 acccgatacc cgaggagata ctcgcgcgct acaacctccc gacgctcacg accgcgctcc
     1981 agtggataca cacgccgaag aaggccgaac acgcgaagag cgcgcggaag cgcttcgcgt
     2041 tcgaggaggt cttctacatt caggcccaga aggcgaagga gcgcgcgtcg gtcgaggcgg
     2101 cggagacgta ccgcttcaag acggaccccg cgcacatcct cgagttcacg aaacgcttcc
     2161 ccttcgacct cacgccgtcc cagataaaag cgctcgccga catcaccgcc gacttcgatc
     2221 ggcgcttccc gatgtcgcgg ctcctcgagg gcgacgtcgg gagcggcaag accgcggtcg
     2281 cggcgaccgc ggcgtacgtc gcggcgacct ctcgtccctc atcgaacgtc tcgaagaaca
     2341 tgggcggcct cgagtacggc aatctccagg tcgcctacat ggcgccgacc gagatactcg
     2401 cgaaacagca cttcgagtcg ttcatcgagt acttcaagca cctccccatc cagatcggcc
     2461 tcatcaccgg ctccggctgc atgaaattcc cctcgaaggt gaacgccgag cgcgcgacgg
     2521 cgatctcgaa ggcgcagctc ctccgctatg tgaaatccgg tgagattccc atcgtcatcg
     2581 ggacgcacgc tctcatgtac aagtcggtcg agtttcggca tctcgcgctc gtcatcatcg
     2641 acgaacagca ccgtttcggc accgcgcagc gaaaggcgct cgcgaagaag gacgcgcggc
     2701 tcccgcacct cctctcgatg accgcgacgc cgatcccccg taccctcgcg ctcaccgtct
     2761 acggcgacct cgacatcacg ctcctctccg agatgccgaa gggccgaaag ccggtcatca
     2821 cgaggctcgt cgcgaagggc cgcgagaagg agatgtacgc gcacgtgaag gaagagctcg
     2881 actcgggccg acaggcgtac gtcatctgtc cgcgcatcga cgaagcggat ccggagaagg
     2941 agacggccat catcgcgaag tcggtcgtcg aggaggcgaa gcgcctcaag aaggacgtct
     3001 tccccgatcg gacgatcgcc gtcctccaca gcaagatgaa accggccgag aaagacaagg
     3061 tcatggcggc cttcgcgaag cacgacatcg acattctcgt cgcgacgtcg gtcgtcgagg
     3121 tcggcgtgaa cgtccccaat gcgacgaaca tcatcatcga gggcgcggag cgcttcggcc
     3181 tcgcacagct tcaccagctc cgcggacgcg tcattcgcgg gacgcaccag ccgtactgct
     3241 tcgtcgtcac cgacagcaag tccgagcgaa cgcgcgagcg cctcaaagcg ctcacgaccg
     3301 cgagaaacgg cttcgaactc gccgagttcg acctcgccca gcgcggttcg ggcgagctct
     3361 acggcgcgaa gcagtcggga ctcaccgacc tcggcatgga ggcgctgaag aacctcaaaa
     3421 tggtcgaagc ggcgcgtacc gaggcgc
//
LOCUS       DASTSP010000022         5469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1159189, whole genome shotgun sequence.
ACCESSION   DASTSP010000022 DASTSP010000000
VERSION     DASTSP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159189"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..764)
                     /locus_tag="VFS75_00940"
     CDS             complement(144..764)
                     /locus_tag="VFS75_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU4677265.1"
                     /translation="MRLSYLVAALLAVIGIAGYYAAGGAGTADIANYPPKNATIVAFG
                     DSLVEGYGATEGNDLVSDLSKELGRPVLNLGRSGDTTEDGLARIGDALAADPGVVILL
                     LGGNDFIRQTDSATTEANLGAIIERLESNGAVVVLLGVRSGVLGGDSAAMYEELAARY
                     RTVYVPDVLSGVIGHGDLMYDGVHPNDAGYAMIAKRVAAAFRKYAL"
     gene            822..1601
                     /locus_tag="VFS75_00945"
     CDS             822..1601
                     /locus_tag="VFS75_00945"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEU4677266.1"
                     /translation="MRDLLETAIDAATDAGSEVMRLYESTDFERKNDGSPVTLADTRA
                     NEVITAKLEATGIAMLSEESASIGHPYPERLWVIDPLDGTRDFIAKSGDFAVMVGLVE
                     NGRPVLGAVFLPVMRKLYFAERGGGAYGRDEKGERRLSVSARTEDLRFVRSGRHVDPR
                     FDAVAKRLAASVTARGSIGVKAGLIAEDESDFLLSWGKLGEWDVCAPEIILTEAGGEV
                     TDAAGQPLRYGNPDHRIQNGMVFSNHACHERVLRAISEVNG"
     gene            complement(1598..1996)
                     /locus_tag="VFS75_00950"
     CDS             complement(1598..1996)
                     /locus_tag="VFS75_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677267.1"
                     /translation="MAFKEIVMGRTTGAFYARLFVAGLVFVGGALMLGRADEGEINVA
                     AAIQNSKEHPSSDTPANERGNANPADANAAAIPAAFRDKPNGGLVPQGGSAPAPAPVA
                     EPVSEAASTTEVSADASIAATTTDAGAEGG"
     gene            2198..2761
                     /gene="infC"
                     /locus_tag="VFS75_00955"
     CDS             2198..2761
                     /gene="infC"
                     /locus_tag="VFS75_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU4677268.1"
                     /translation="MKDERVLINQQIRARELRVIDAEGENRGVISKEEALKLAEEAGL
                     DLIEISPMANPPVAKIMDFGKYQYELKKKAKEVKAKSHVTETKSLQVKIGTGEHDLAL
                     KAKRAAGWLEEGHRVKIDLFLWGRYKYMDEAFLKERLGRFLKIIPAEYRIADEIKKSP
                     KGFSTTIERAAQKGAKAKESDTHHENK"
     gene            2748..2933
                     /locus_tag="VFS75_00960"
     CDS             2748..2933
                     /locus_tag="VFS75_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU4677269.1"
                     /translation="MKTNKSYTKRIKVTKNGKLVARKAGQNHFNSRQTGRQRQNRSRT
                     QTLSITKRARNRFLANA"
     gene            2962..3306
                     /gene="rplT"
                     /locus_tag="VFS75_00965"
     CDS             2962..3306
                     /gene="rplT"
                     /locus_tag="VFS75_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU4677270.1"
                     /translation="MARVKGGVQAQKRRRKILSQVKGFRLGRSKKKRMAIEAIHHAHL
                     HAFAHRKDKKNDFRRLWTVRLNAALKAAGMKYSVFMGSLSKKGLSLNRKMLAEMAATR
                     PAAFERLLANIK"
     gene            complement(3355..4716)
                     /locus_tag="VFS75_00970"
     CDS             complement(3355..4716)
                     /locus_tag="VFS75_00970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4677271.1"
                     /translation="MTAKLSFMGGAGSVTGANFLLDLGEETIMVDCGLVQEGRFSMEK
                     NCEAFAYDPSAVGTLFVTHAHADHIGRIPKLVRDGFRGIIYSTPPTRDLAAVMLEDAY
                     HVMASNRERYSTDTCFELEDIAAALRLWKTVEYHEPVGLSSGATATFTDAGHILGSAM
                     VTIERKGKRIAFTGDIGNVPQPLLNPPEALHDYDYLVMESVYGDRLHEGVEKRAELLR
                     EHLEDTVRKGGTLIIPAFSLERTQGMLFEINNLFESGAMKPIPVFLDSPLAIRVTGLY
                     KKYTNYLKDAVRAQIEGGDDVFDFPRLAMTETAEASRGIDAVHGAKVIIAGSGMSHGG
                     RILAHEIKYLEDPKTTLLLVGYQAVGSIGRLLGDGAKKVKILDRQVRVRAKVAQIHGY
                     SGHADRDQLVEFVGEGSGRVRQVFVTMGEERSSLFLVQRLRDYLGVRAVAPELGSSVD
                     IDL"
     gene            complement(4713..5102)
                     /locus_tag="VFS75_00975"
     CDS             complement(4713..5102)
                     /locus_tag="VFS75_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677272.1"
                     /translation="MTLRLTLFFTAFITLAIVHLAAVRFFLYWKYLWLDIPMHALGGA
                     VVMLGLFILPFFRIALPARLSSFSGSLALVLLVGVLWELFEVAFGLSLPPGSHESYAA
                     DTLTDLVMDLMGGAAGYLVAKSTYHLS"
BASE COUNT         1123 a         1635 c         1758 g          953 t
ORIGIN      
        1 cggcgatcgg cgtcgcggtc aatgaatccc agaggaagaa aacgacgaga aggaggagga
       61 cgccgagcgt gacggccatg atgagggcgc ctgttttcga ggaacgctcg gagggcatgc
      121 gtatatgata gcaggtcgcg gggctagagc gcgtacttcc taaacgccgc ggcgacgcgc
      181 ttcgcgatca tcgcgtaccc cgcatcgttc gggtggacgc cgtcgtacat gaggtcgccg
      241 tggccgatca cgcccgagag aacgtcgggg acgtacaccg tgcggtaccg cgcggcgagc
      301 tcctcgtaca tggcggcgct atcgccgccg aggacgccgc ttcgaacgcc gagaaggacg
      361 acgacggcgc cgttcgactc gagcctctcg atgatcgcgc cgaggttcgc ctccgtcgtt
      421 gccgaatccg tctgcctgat gaagtcgttc cctccgagga ggaggatgac gacgccgggg
      481 tcggccgcga gggcgtcgcc tatgcgggcg agaccatctt cggttgtgtc gcccgagcgg
      541 ccgagattga ggactgggcg accaagctct ttcgaaaggt ccgagacgag gtcgttcccc
      601 tcggtcgctc cgtagccctc gacgagcgag tcgccgaacg cgacgatggt cgcgttcttc
      661 ggcggatagt tcgcgatatc ggccgttccc gcgccgcccg cggcatagta cccggcgatg
      721 ccgatgaccg cgaggagagc cgcgacgagg tacgaaagac gcataggagc tagtgtatca
      781 aaaggtgcgc gcgaacgctt ctctgctata ctccgtctat catgcgagac cttctcgaga
      841 cggcgatcga cgcggccacc gatgcgggca gcgaggtcat gcgcctctac gaatcgaccg
      901 acttcgaaag aaagaacgac gggagcccgg tcaccctggc cgacacgcgc gcgaacgagg
      961 tcatcaccgc aaaactcgag gctaccggta tcgccatgct ctccgaggag tcggcgtcga
     1021 taggtcaccc gtaccccgag cgcctctggg tcatcgatcc cctcgatggt acgcgcgact
     1081 tcatcgcgaa gtcgggtgat ttcgccgtca tggtgggact cgtcgagaac gggcgtcccg
     1141 ttctcggtgc ggtcttcctc cccgtcatga gaaagctcta cttcgcagag cgcggcgggg
     1201 gtgcgtacgg gagagacgag aagggtgaaa gacggctctc cgtctccgcg cgcaccgaag
     1261 accttcgctt cgtgcgaagc ggccggcacg tcgacccacg tttcgacgcg gtcgcaaagc
     1321 gactcgcggc gtcggtcacg gcgcgaggga gcatcggcgt aaaggcgggg ctcatcgccg
     1381 aagacgagag cgacttcctt ctctcatggg ggaagcttgg cgagtgggac gtctgcgcgc
     1441 ccgagataat cctcacggag gcgggcggcg aagtgacgga cgcggcaggc cagccgcttc
     1501 gttatgggaa tccggaccac cgcatacaaa acggcatggt cttctcgaac catgcctgtc
     1561 atgagcgcgt tcttcgcgcg atcagcgagg tgaacggcta acctccttcc gctccggcgt
     1621 ccgtcgtcgt cgcggcgatg gacgcgtccg ctgatacctc ggtcgtcgaa gcggcttcgg
     1681 agacaggctc cgcgacgggc gcaggcgccg gtgcgcttcc tccctgaggg acgaggccgc
     1741 cgtttggctt atctctgaag gccgcgggga tcgccgcggc gttcgcgtca gcgggattcg
     1801 cgttcccccg ctcgttcgcc ggtgtgtcgg acgatgggtg ctctttcgag ttctggatag
     1861 ccgcggcgac gtttatctca ccctcgtccg cgcggcccag catgagcgcg ccgcccacga
     1921 agacgagtcc ggcgacgaaa agccgcgcgt agaaggctcc cgtcgtcctc cccattacga
     1981 tttccttgaa cgccatagtg cctcaagtat accatcatgg gcgcggtcgg aaaggccacc
     2041 ctgctgtgta cgcgtccggc cgtgatattg ccgttttccg cggtatatgg tacatttgcc
     2101 acaccgagag agcaggggct ctctttttgc tatcgaagaa cggggcgcga aacggcgcca
     2161 ccgatacgat aggccggaac ctttactttt catcactttg aaagacgaac gagtactcat
     2221 aaaccagcag attcgcgcac gcgaacttcg cgtcatcgac gcagagggcg agaaccgggg
     2281 cgtcatctcg aaggaggaag cgctcaaact cgcggaggaa gctgggctcg atctcatcga
     2341 gatctccccg atggcgaacc cgcccgtcgc gaagatcatg gacttcggga agtaccagta
     2401 cgagttgaaa aagaaggcga aggaggtgaa ggcgaagtcg cacgtcaccg agacgaagag
     2461 tctccaggtg aagatcggca ccggcgagca cgatttggcg ctcaaagcca agcgcgcggc
     2521 cggatggctc gaggaaggtc atcgggtgaa gatagacctc ttcctctggg gacggtacaa
     2581 gtacatggac gaggccttcc tcaaggaacg gctcggccga ttcttgaaaa tcatacccgc
     2641 cgagtataga atagccgacg aaatcaagaa gagcccgaag ggattcagca cgacgatcga
     2701 gcgtgcggcg cagaaaggcg cgaaggcaaa ggagtcagat acacaccatg aaaacaaata
     2761 aatcatacac gaagcgcatc aaggtcacga agaacggaaa gctcgtcgcg cgcaaggcgg
     2821 ggcagaacca cttcaattcg cgtcagaccg gccgccagcg gcagaaccgg agccgaacgc
     2881 agaccctttc gatcacgaag cgcgcacgaa accgcttcct cgcgaacgcg taacatttcc
     2941 agtaagtagt aacgaatacc tatggcacga gtaaaaggag gagtacaggc gcaaaagcga
     3001 cgacgaaaga tcctttcgca ggtgaagggc tttcgcctcg gccgctcgaa gaagaagcgc
     3061 atggcgatcg aagcgatcca ccacgcgcac ctgcacgcgt ttgcgcaccg aaaggacaag
     3121 aagaacgact tccgacgcct ctggacggtc cgcctcaacg ccgctctcaa ggctgcgggg
     3181 atgaagtaca gcgtcttcat gggctcgctc tcgaagaagg ggctttccct caaccgaaag
     3241 atgctcgcgg agatggcggc gacgcggccg gccgcgttcg agcgactcct cgcgaacatt
     3301 aagtaacctt ttccctttca aaacccccgg gcttcgcccg ggggttttcg ttggtcagag
     3361 gtcgatgtcg acggagctcc cgagctccgg tgctaccgca cgcacaccga ggtagtcgcg
     3421 gagtcgctga acgaggaaca aggaggagcg ctcctctccc atcgtcacga agacctgccg
     3481 aacgcggcct gacccctcac caacgaactc gacgagctga tcgcggtcgg catgccccga
     3541 gtacccgtga atctgcgcga ccttcgcgcg cacgcggacc tgtcggtcga ggatcttgac
     3601 cttcttcgcg ccgtcgccga ggaggcggcc gatggagccg accgcctggt agccgacgag
     3661 gaggagggtc gtcttcgggt cctcgaggta cttgatctca tgcgcgagaa tgcggccccc
     3721 gtggctcata ccggagcccg cgatgatgac cttcgcgccg tgtaccgcat cgatgccgcg
     3781 cgacgcctcc gccgtctccg tcatggcgag ccgcgggaaa tcgaagacgt cgtcgccccc
     3841 ttcgatctgg gcgcgaaccg catccttgag gtaattcgtg tactttttat agaggcccgt
     3901 cacgcggata gccaagggcg aatcgaggaa gaccgggatg ggtttcattg ccccgctctc
     3961 gaagaggttg tttatctcga agagcatacc ctgcgttcgc tcgagcgaga acgccgggat
     4021 gatgagcgtg ccacccttcc tcaccgtgtc ctcaaggtgc tcgcggagga gttcggcgcg
     4081 cttttcgacg ccctcgtgga gccggtcgcc gtagaccgac tccatgacga ggtagtcgta
     4141 gtcgtggagc gcttcgggcg ggttgagaag cggctggggc acgtttccga tgtcgccggt
     4201 gaaggcgatg cgcttcccct tccgttcgat ggtcaccatc gcggagccga ggatgtggcc
     4261 ggcgtcggtg aaggtcgccg tcgcgcccga ggagagaccg acgggctcgt ggtactcgac
     4321 ggtcttccaa aggcggagcg ccgccgcgat gtcctcgagc tcaaagcagg tatccgtgct
     4381 gtaccgctct cggttcgacg ccataacgtg gtatgcgtcc tcgagcatga ccgcggcgag
     4441 atcgcgggtc ggcggcgtcg agtagatgat gccccgaaag ccgtctcgaa cgagcttcgg
     4501 aatgcggccg atatggtcgg cgtgcgcgtg cgtgacgaag agcgtgccga ccgcggacgg
     4561 gtcgtacgcg aacgcttcgc agttcttctc catcgagaac cgtccttcct gaacgagtcc
     4621 gcagtcgacc atgatagtct cttcgccgag gtcgaggagg aagttcgctc ccgtcaccga
     4681 cccggccccg cccatgaatg agagtttcgc cgtcatgaga gatggtacgt gcttttcgcg
     4741 acgagatatc ccgccgcacc gcccatgagg tccatgacga ggtcggtcaa cgtgtcggcc
     4801 gcgtacgatt catgcgagcc gggagggagc gagagcccga aagcgacctc gaagagctcc
     4861 cagagcaccc caacgagaag gacgagcgcg agcgagccgg aaaacgacga gaggcgcgcc
     4921 ggaagcgcga ttcggaagaa cggcagtatg aataggccga gcatgacgac cgcgccgccg
     4981 agggcgtgca tcgggatgtc gagccagagg tatttccagt agaggaagaa gcgcaccgct
     5041 gcgagatgca cgatggcgag cgtgatgaac gcggtgaaga agagggtaag ccgaagcgtc
     5101 atgggtgtat ggtagcacgc cgagcgagca ctaaaaaaac gtaccctggg tacgtttttt
     5161 tagtggagcg acggttgcat gtttcctgac tctagatcag gtgggcaccc tttccaagca
     5221 cttttgaaat ctcgtcgcgt cgagttctca gctgtacttg tgactacggg cagccctgga
     5281 tgttctttaa gcaagaacat gcgggaccga cgctccacag aaagtataac agaaaacgcg
     5341 aaccgatgcg gttcgcgttc tcgtctttgt gcacaagtct actccttcgt agacgggtcg
     5401 aggtcgatgt cgtacatgat gcgctcctgc gccgtcacat agtccatgat ctcgccgtcc
     5461 gtgaaccag
//
LOCUS       DASTSP010000023         6252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_45335, whole genome shotgun sequence.
ACCESSION   DASTSP010000023 DASTSP010000000
VERSION     DASTSP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45335"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFS75_00980"
     CDS             <1..351
                     /locus_tag="VFS75_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677273.1"
                     /translation="ELVRLARESFHKVFKRGTPYRATGAVLMDLGPERSLQLDLFGSV
                     ASAEKWKPVYATVDGLDERFGKHTVYLGSTWKAMHTKAHENERGDAPARAKELLRGEN
                     ARQRIGIPMLGDVT"
     gene            complement(394..3078)
                     /locus_tag="VFS75_00985"
     CDS             complement(394..3078)
                     /locus_tag="VFS75_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4677274.1"
                     /translation="MPNFHNFTSRAKEAIRKAHELAIERGQNHVSPTHLLTALVMQDE
                     NTVISLLDRLDIDIIALTDLLLEALEMPGSAQTLSQSYQLYLTPDLAQALEQSGRIAQ
                     SLGDSYVGVEHLFLALLEHPGPAIEVIERAHIDKDKVQKALKEIREHNIEDAQSPAKK
                     FRALSKYARNLNKLAQENKLDPVIGRDAEIQRVIQILSRRTKNNPVLIGEAGVGKTAI
                     AEGLALAIVQGNVPESVKGKEVLSLDLGLLVAGTKFRGEFEERLKAVMKEIEQAQGSV
                     ILFIDELHTIVGAGQAEGSMDASNMLKPALARGEMRVIGATTLKEYQKHIERDPALTR
                     RFQPVYVNEPSIEDAIAIMRGVAEKYEIYHGVRITDDAIVAAVNLSSRYISDRFLPDK
                     AVDLIDEAASALRISLENKPEELVVADRAVRRLEIEREALNKEEETSEHKPKIKARLK
                     EITEEIGELKQQTSTLETKWNNEKDAVTEIREMKKQLEELRLEAEQAEASADLTRAAE
                     IRYALVPKIEKDLEAKSSRLKKLQRTRRLLKEEVTEEDIAAVVSRWTGIPVSRMLEAE
                     AKKLARMEEVLSERVVGQTEAVKRVSDAIKRSRAGIADPNRPIGSFLFLGPTGVGKTE
                     LSRALAEFMFNDESALVRIDMSEYMEKHSVSKIIGSPPGYVGHEEGGAITELVRHRPY
                     SVILLDEIEKAHPEVFNILLQVLDSGHLTDAKGRKVNFKNTVIIMTSNIGAEHIDRMS
                     KFGFTNQTDEASQYAQAKDKVLESLKNFFRPEFLNRLDEIIIFDILSKETIRNIVNIQ
                     VRQVIDRLAQKEIKLTVTPEVLDYLAKEGYDPRFGARPLKRVIQSKILTPVASVMVGE
                     GMLQGGTVAVSMKKGELHFDVRKKSRPAASRKGEKVAV"
     gene            complement(3180..4406)
                     /locus_tag="VFS75_00990"
     CDS             complement(3180..4406)
                     /locus_tag="VFS75_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU4677275.1"
                     /translation="MGFSSLFRKPRVHLDYASATPVSERVFDAMRPYFRDVWANPSAV
                     YEEGVIARAAIERARTDLARTLHVRPAGITWTSGGTEANNLAIVGTIEARRKDGMAYE
                     EMEVLSMRIEHPSILETLTRLKERGVRVTYLPVDGDGLVSVDDVSRALSKRVVLVTYA
                     YVNSEIGVIQDVKGISRAVRKWNDANEGTILTHVDASQAPLWLPCALDSLGVDMMTLD
                     AGKCYGPKGVGALVRRAHVALEPVLFGGGQESGLRSGTENAALIIGAARAFQDAQSEH
                     EALAKRVATLRDGAFDRILKEFPDAVVNGSKESRVANNVNVSFPGTDVEYAVIWLDAK
                     GIATSTRSACGAAGKNGSDVVREMTKDEARALSTIRFTFGRESTMSDISRAIHALKAY
                     NSVMYGKDGVRGRGKS"
     gene            4433..6007
                     /gene="infB"
                     /locus_tag="VFS75_00995"
     CDS             4433..6007
                     /gene="infB"
                     /locus_tag="VFS75_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU4677276.1"
                     /translation="MWQYLLYSRIHSNTPCHGAFFNTETRMPKQPAQQKRVPRPPIVV
                     VMGHIDHGKSTLLDHIRETNVVAGEAGGITQHLSAYRVEHETKDKAKKLITFLDTPGH
                     EAFQKMRHRGADVADIAILVVSAEDGVKPQTMEALDSILKAGIPYIVAINKIDKPSAN
                     LVMTQSSLIEHGIYIEGMGGDISWAAISAKTGQGVDELLDLVLLTAELAELTTDPSLP
                     GAGSVIEGKLDQKRGNTATLMVKDGTVRTGDFVVSGSCYAPVRIMEDASGTSIKEAGA
                     SEPIGIVGWNEVPRIGDVFTTVSSKKEAEKLAAENTIAAPKALSDESDLPTIPLLIKA
                     DVMGTIDAVVHELEKYKSDRVLVRIIGTGVGSISSNDVQNVSATKNAIIVGFNVTPDR
                     AAIDLAERLGVEIQSFNIIYKLSEWLEEALKVRTPKKEEERITGKAKILKHFSTQKGS
                     HVLGGRLEVGTLALNQQVRITRRDVELGRGVLKNLQQQKSNVKEISDGEFGMQLDSKV
                     DVAPGDYIEAFDVVII"
     gene            6009..>6252
                     /locus_tag="VFS75_01000"
     CDS             6009..>6252
                     /locus_tag="VFS75_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677277.1"
                     /translation="MEFDRHDKARSLFMELAAAFVRDEANTNPLITVTNVDVSPSYQH
                     VKVFFTTIPDGKEDDALIFLKRKGSDLREYIKKHSRL"
BASE COUNT         1216 a         1963 c         1859 g         1214 t
ORIGIN      
        1 gagctcgtcc gcctcgcccg cgaatcgttt cataaggtgt tcaaacgagg cacaccctac
       61 cgtgcgacgg gcgcggtcct catggactta gggcccgaac gctcgcttca gctcgacctt
      121 ttcgggtcgg tcgcgtccgc ggagaagtgg aaacccgtct acgcgacggt agacggcctc
      181 gacgaacgct tcgggaagca cacggtgtat cttggctcga cgtggaaggc gatgcatact
      241 aaagcgcacg agaacgagcg gggcgatgcg cccgcgcgcg cgaaggagct cctccgcggc
      301 gagaacgccc gccagcgcat cggcatcccg atgctcggcg acgtcacgta aaaagcgccc
      361 acaccggggc gctttttagg gttatcagta tccttacacc gcgaccttct ctccctttcg
      421 actcgcggcg ggacggctct tctttcgaac gtcgaagtgg agttccccct tcttcatcga
      481 cacagcgacg gtgccgccct ggagcatgcc ttcgccgacc atgacggacg cgaccggcgt
      541 gaggatcttc gactggatga cgcgcttcag cggtcgcgcg ccgaaccgcg gatcgtagcc
      601 ctccttcgcg aggtagtcga ggacctccgg cgtcaccgtg agcttgatct ccttctgcgc
      661 gagtcggtcg atgacctggc gcacctgaat gttcacgatg ttcctaatgg tctccttcga
      721 gagaatatcg aagatgatga tctcatcgag acggttgagg aactcggggc ggaagaagtt
      781 cttgaggctc tcgagcacct tatccttcgc ctgcgcgtac tgcgaggctt cgtcggtctg
      841 attcgtgaag ccgaacttcg acattcgatc gatgtgctcc gcgccaatgt tcgaagtcat
      901 gatgatgacc gtattcttga agttcacctt ccttcccttc gcgtcggtga ggtgaccgga
      961 gtcgaggacc tggagaagga tattgaacac ctccggatgc gccttttcaa tctcgtcgag
     1021 aaggatgacc gagtacggtc ggtgacgtac gagctcggtg atggcgccgc cctcctcgtg
     1081 tccgacgtac cccggcggcg acccgatgat cttcgagacg gaatgcttct ccatgtactc
     1141 ggacatgtcg atgcggacga gcgcgctctc atcattgaac atgaactcgg cgagggcgcg
     1201 agagagctcg gtcttgccaa cgcccgtcgg accgaggaag aggaacgaac cgatggggcg
     1261 gttcgggtcg gcgatgccgg cgcgcgaacg cttgatcgcg tcggatacgc gcttcaccgc
     1321 ctccgtctgt ccgacgacgc gctccgagag aacctcctcc atacgcgcga gcttcttcgc
     1381 ctcggcttcg agcatgcgtg agaccggaat gccggtccag cgcgagacga ccgccgcgat
     1441 gtcctcctcg gtcacctcct ccttgagaag acggcgcgtt cgctggagct tcttgagtcg
     1501 cgacgatttc gcctcgaggt ccttctcgat cttcggaacg agcgcgtagc gaatctccgc
     1561 cgcgcgggtg aggtctgccg acgcctcggc ctgctcggcc tcgagccgga gttcctcgag
     1621 ctgcttcttc atctcgcgta tctcggtcac cgcgtccttc tcgttgttcc acttcgtctc
     1681 gagggtcgag gtctgctgct tcaattcacc gatctcctcg gttatctcct tgaggcgagc
     1741 ctttatcttc ggcttatgct ccgacgtttc ctcttcctta ttgagcgcct cgcgttcgat
     1801 ctcaagacga cggacggctc ggtcggcgac gacgagctcc tcgggcttgt tctcaaggga
     1861 gatacggagc gccgaagcgg cctcatcgat gaggtcgacc gccttatcgg gaaggaagcg
     1921 gtcgctgatg tagcgcgatg agagattcac cgcggcgacg atggcgtcgt cggtgattcg
     1981 aacgccgtgg tatatctcgt acttctcggc gacgccgcgc atgatggcga tcgcgtcttc
     2041 gatcgatggt tcgttcacgt agaccggctg gaaacgacgc gtaagcgccg ggtcgcgctc
     2101 gatgtgcttc tggtattcct tgagagtcgt cgcaccgatg acgcgcatct cgccgcgcgc
     2161 gagggcaggt ttcaacatgt tcgacgcgtc catagaaccc tccgcctgac cggcgccgac
     2221 gatggtgtga agctcgtcga tgaagaggat gacggaaccc tgtgcctgct cgatctcctt
     2281 catgactgcc ttgagacgct cctcgaactc gccgcggaac ttcgttccgg cgacgaggag
     2341 gccgaggtcg agggagagga cctccttacc cttcacggac tcgggaacgt tcccctgaac
     2401 gatggcgagc gcaagtccct ccgcgatcgc ggtcttgccg acgcccgcct caccgatcaa
     2461 taccgggttg ttcttcgtgc ggcgcgagag tatctgaatg acgcgctgta tctccgcgtc
     2521 gcgtccgatg accggatcga gcttgttctc ctgtgcgagc ttattcaaat tgcgcgcgta
     2581 cttcgagagc gcacgaaact tcttcgcggg cgactgcgcg tcctcgatat tgtgctcgcg
     2641 gatctctttg agcgccttct gcaccttatc cttgtcgatg tgcgcgcgct cgatgacctc
     2701 gatcgcggga cccgggtgtt cgagaagcgc gaggaagagg tgctcgacgc cgacgtacga
     2761 atcgccgagg gactgcgcga tgcggcccga ctgctcgagg gcctgcgcga ggtcgggagt
     2821 gaggtaaagc tggtacgact gcgacagcgt ctgcgcagag cccggcatct cgagagcctc
     2881 gaggagaagg tcagtgagcg cgatgatatc gatatcgaga cggtcgagga gcgagatgac
     2941 ggtgttctcg tcctgcatga cgagagcggt gaggaggtgc gtcgggctca catggttctg
     3001 cccgcgctcg atggcgagct cgtgcgcttt gcgtatcgct tccttcgctc tcgatgtgaa
     3061 gttgtgaaag tttggcatgg tgaaagattc attatacgcg agagcgcccc gtccaaagaa
     3121 cctaaaaggc gaaccccaaa actggtaata gccttataat acaacgcttt tacgggaagt
     3181 caagacttcc ctcgaccccg aacgccatcc ttcccgtaca tgacactatt atatgccttg
     3241 agggcatgaa tagcccgtga aatatccgac atcgtggact cccttccaaa ggtaaaccga
     3301 atggtcgaga gcgcccgggc ctcgtccttc gtcatctcgc gaacgacgtc cgagccgttc
     3361 ttgccagccg cgccacacgc gcttcgcgtc gacgtcgcga tccccttggc gtcgagccat
     3421 atgaccgcat actccacgtc cgtgcccggg aaggagacgt tcacgttgtt cgcgacacgc
     3481 gactccttgc ttccgttcac gaccgcgtcg ggaaattcct ttagaatcct gtcgaacgcg
     3541 ccatcccgaa gagtggctac gcgtttcgcg agcgcttcat gctccgactg tgcgtcctga
     3601 aacgcgcgcg ccgcgccgat gatgagcgcg gcgttctcgg tacccgaacg caacccggac
     3661 tcctgtcccc cgccgaagag gacgggctcg agcgcgacat gcgcgcgccg gacgagcgcg
     3721 ccaacgccct tcggtccata gcacttcccc gcatcgagcg tcatcatatc gacgccaagg
     3781 gaatcgagcg cacagggaag ccacagcggc gcctggctcg cgtctacgtg cgtgaggata
     3841 gtcccctcgt tcgcatcgtt ccactttcgt acggcgcgcg agataccctt cacgtcctgt
     3901 atcactccga tctccgagtt cacgtacgcg tacgtgacga ggaccacccg cttcgagagc
     3961 gcacgcgaga cgtcatcgac gctcacgaga ccgtccccgt cgacgggaag gtacgtcacg
     4021 cggacgcctc tctccttaag gcgcgtgagc gtttcgagaa tggagggatg ctcgatgcgc
     4081 atcgagagca cctccatttc ctcatacgcc ataccatcct ttcgacgcgc ttcgatggtt
     4141 cccacgatgg cgaggttgtt cgcctcggtg ccgccagacg tccacgtgat gcccgcgggc
     4201 cggacatgaa gcgtgcgagc caggtccgtt ctcgctcttt ctatggccgc gcgagcgatg
     4261 actccttcct catagacggc gctcgggttc gcccacacat cgcgaaaata gggacgcatc
     4321 gcgtcgaaga cgcgctccga tacgggcgtc gcggacgcgt agtcgaggtg cactcgcggc
     4381 tttcgaaaga gggaggaaaa ccccatttcg tgatgatgtc acagggtgcc atttgtggca
     4441 atatctgcta tactctcgca tacattcgaa tacgccttgt catggggcgt ttttcaatac
     4501 cgagactcgt atgccgaaac aacccgcaca acagaagagg gtcccccgcc cgcccatcgt
     4561 cgtcgtcatg ggccatatcg accacggcaa gtcgacgctc cttgaccata tccgcgagac
     4621 gaacgtcgtc gccggcgagg cgggcggcat cacgcagcat ctctccgcgt accgcgtcga
     4681 gcacgagacg aaggacaagg cgaagaagct catcacgttc ctcgatacgc cgggccacga
     4741 ggcgttccaa aagatgcgac accgcggcgc ggacgtcgcc gacatcgcga tcctcgtcgt
     4801 ctccgccgag gacggcgtaa agccgcagac gatggaagcg ctcgattcga tcttgaaagc
     4861 gggcattccg tatatcgtcg ccatcaacaa gatagacaag ccgagcgcga acctcgtcat
     4921 gacgcagtcg agcctcatcg agcacggcat ctacatcgaa gggatgggcg gggacatctc
     4981 gtgggcggcg atctccgcga agacgggcca gggcgtcgac gaactcctcg acctcgttct
     5041 cctcaccgcc gagctcgccg aactcacgac cgacccttcc cttccgggag ccggaagcgt
     5101 catcgagggc aagctcgacc agaagcgcgg caacaccgcg acgctcatgg tgaaggacgg
     5161 gacggtccgt accggcgact tcgtggtctc gggctcgtgc tatgcgccgg tccgcatcat
     5221 ggaggacgcg agcggcacga gcatcaaaga ggccggcgcg tctgaaccga tcggcatcgt
     5281 cggctggaac gaggtgccgc ggatcggcga cgtcttcacc acggtctcgt cgaagaaaga
     5341 agcggaaaag ctcgccgccg agaataccat cgccgctccg aaagcgctct cggacgaatc
     5401 cgacctcccg accatccccc ttctcatcaa ggcggacgtc atgggcacga tcgacgccgt
     5461 cgttcacgag ctcgagaagt acaagtccga ccgcgtcctc gtccgcatca tcggcacggg
     5521 cgtcggctcg atatcctcga acgacgtcca gaacgtgagc gccacgaaga acgccatcat
     5581 cgtgggattc aacgtaacgc ccgaccgcgc cgcgatcgac ctcgcggaac ggctcggcgt
     5641 cgagatacag tcgttcaata tcatctataa gctctcggag tggctcgagg aggccttgaa
     5701 ggtccgtacg ccgaagaagg aggaagaacg tatcaccggg aaggcgaaga tcctcaagca
     5761 tttcagcaca cagaagggct cgcacgtcct tggcggacga ctcgaggtag ggacgctcgc
     5821 cctcaaccaa caggttcgta tcacacgccg tgatgtcgag ctcgggcgcg gcgttctcaa
     5881 gaacctccag cagcaaaagt cgaacgtcaa agagatctcc gacggcgagt tcggcatgca
     5941 actcgactcg aaggtcgacg tcgcgcccgg cgactatatc gaggcgttcg acgtcgtcat
     6001 catttgatat ggaattcgat cgacacgaca aagcgcgttc cctcttcatg gagctcgccg
     6061 cagcgttcgt ccgcgacgag gcgaacacga accccctcat caccgtgacg aacgtcgacg
     6121 tctcgccgag ctaccagcac gtgaaggtct ttttcacgac gatccccgat gggaaggagg
     6181 acgacgcgct catcttccta aagcggaaag gatcggacct tcgcgagtac atcaaaaagc
     6241 atagccgcct ga
//
LOCUS       DASTSP010000024         6167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1607571, whole genome shotgun sequence.
ACCESSION   DASTSP010000024 DASTSP010000000
VERSION     DASTSP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607571"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFS75_01005"
     CDS             complement(<1..547)
                     /locus_tag="VFS75_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4677278.1"
                     /translation="MRVLTFGWDYPPMKNGGLGVACHGLTEELIDSGVEVLFVLPHEQ
                     ETIGSGRFLFANARPGQARIERISSPLKPYQASDSFIEMFLPGGQRVRFSRSVLEEVY
                     RYAEEAARIAQTEHFDLIHAHDWTAYLAGMVAKKVSLKPLVLHVHATSYDQAGGGNVD
                     PEIYKIERTAFSAADSVVAISN"
     gene            complement(681..1697)
                     /locus_tag="VFS75_01010"
     CDS             complement(681..1697)
                     /locus_tag="VFS75_01010"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEU4677279.1"
                     /translation="MENENLYKTVAQLMQAGKGILAADESDSTAGKRLAIAKLPNQPG
                     NRQDFRELLFTAPGIEEFLSGIILYDTTLRESTDQGVPFVDVLTARGIVPGIKVDKGT
                     RPLPQFPDEVVTHGLDGLDERFKEYYEIGARFAKWRAVVTIGDTIPTDAALEMNADRM
                     AEYALLSQAAGLVPMVEPEVMFPGTHSLEVAEEVTTRTLTILFKKLREFRVDLKGLLL
                     KSSMVLAGDRHPDQSTPDEVAAATLRTFKLSVPREVGGIVFLSGGQTPKRATENLQAI
                     AAFGPQSWPITFSYSRAIQEPVLVAWQGKPENVGAAQKTLLQYVKQNGLAQLGKYETS
                     KDGK"
     gene            complement(1769..2539)
                     /locus_tag="VFS75_01015"
     CDS             complement(1769..2539)
                     /locus_tag="VFS75_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4677280.1"
                     /translation="MNGINWIGIYTMIRREMMRMLRVVIQTLVSPLISATLYIFIFGH
                     VIGSRISLIAGVPYITFVFPGILMLNIIMASFSHASSSVYMGRFIKSIEEILIAPFSY
                     FEIVFGFAAAAVIRSTLVAVGILAIGVLFHAVTITSPFLFLFYVVAISTIFALTGILV
                     GLWANGFEQLSALNTFVITPLTYLGGIFYSISMLPPVMAHITRWNPFFYFADGIRHSM
                     IGFSEANAYVGLAVILGLVAVLGFVTIYLFRIGWRIRS"
     gene            complement(2536..3267)
                     /locus_tag="VFS75_01020"
     CDS             complement(2536..3267)
                     /locus_tag="VFS75_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4677281.1"
                     /translation="MAETDILVIEHLVKKYGEKTAVDDVSLSIRRGDFYGFLGPNGAG
                     KSSTIGCIIGVNKITSGRILVDGLDVVEDYKEARKRIGVSSQEYNVDFFASVEDLIDF
                     LGGYYGIPANVRRERLEALLVRFNLVEHRKKAFRELSGGLKRRVILARALIHDPMLLI
                     LDEPTAGVDVEQRHELWRYLTELNEEGKTIILTSHYLEEVERLCRHITIINKGKLVAT
                     GTREEFVEGGRSLEERYLEITKQAI"
     gene            3303..4073
                     /locus_tag="VFS75_01025"
     CDS             3303..4073
                     /locus_tag="VFS75_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HEU4677282.1"
                     /translation="MNMTRDEEWLLREKYEGDMNPSFVEDCARLRAGEPLAYVIGHVP
                     FLGVTVYLDSRPLIPRAETEFWVERAIAEMPPHEPIRVLDLCSGSGAIGLAVLAKRLN
                     ASVDLIELEERHHATIERNLRENGVSASRARVLGGDLFERAEGPYDHILSNPPYIDPA
                     LDRVAQSVRAFEPAEALYGGEGGLELVRRIMREAEGFLAPYGRLYIEHEPEQSAAIAT
                     AAKEAGLACRTERDQYGVDRYSVLSCLAPRFHVESSHG"
     gene            4066..5310
                     /gene="hisS"
                     /locus_tag="VFS75_01030"
     CDS             4066..5310
                     /gene="hisS"
                     /locus_tag="VFS75_01030"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU4677283.1"
                     /translation="MDKKLSTEPYKGTRDFYPEDMAVERYIFDTWAKVAESFGFERYD
                     ASILEPSELYRSKGAENEELVNEQTYTFVDRGEREVTLRPEMTPTVARMVAGKRRELS
                     FPVRWYSIPNLFRYERPQRGRLREHWQLNCDMFGVDDVASDIEMIALGYRILLAFGAS
                     PKMFEIRVNDRTWLKLQLSDYGLSEEQMKAMLTLIDKRDKIDDFDEEAEKIAGKKVSL
                     DFTTPNPEHRLMQVIEGLKALGIENVVISPSTVRGFSYYTGTIFEFFDTASENNRSLL
                     GGGRYDNLTALFGGDPVSGVGFGMGDVTMRDFLETHSLLTKDVKTTAPTLAILPLDAT
                     HNLAAEVLAEEFRSAGVRTAVDLSDRKVGKKLSAASDAGVAYVIVLGEDEAASRSYAL
                     KNLGTGDETKGSAEELAKHIAR"
     gene            5311..5886
                     /locus_tag="VFS75_01035"
     CDS             5311..5886
                     /locus_tag="VFS75_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU4677284.1"
                     /translation="MRYVVFDLETQNIFSDVGSNDPSDLDVSVGSAYDSGTDTYTTVT
                     VDELHKLWPIFEQADLLVGYNSDHFDIPLLNKYYPGDLTTIKSVDLCSAVKDSLGKRL
                     RLDSLAQATLGSKKSGNGLMAIKWWREGDIASIKKYCEQDVKITKGIFEYALQNGKLL
                     YKDGHRKKEIPIDTSSWTPHEEHSMTFSLPF"
     gene            complement(5913..>6167)
                     /locus_tag="VFS75_01040"
     CDS             complement(5913..>6167)
                     /locus_tag="VFS75_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU4677285.1"
                     /translation="AARFSFLLGLPVIVGSGLKMLLELISSGEDVAWFSVFVGSIVAF
                     VVGLISIRFLLSFIRKHSLWPFIWYRIALACFVLFVTFFG"
BASE COUNT         1272 a         1892 c         1879 g         1124 t
ORIGIN      
        1 agttgcttat cgcgacgacc gaatcggccg cagaaaacgc cgttcgctct attttgtata
       61 tctccgggtc gacgttcccg ccgcccgctt ggtcgtacga cgtcgcatga acgtggagga
      121 cgagcggttt gaggcttacc ttcttcgcga ccatgcccgc gaggtacgcg gtccaatcgt
      181 gcgcgtgaat gaggtcgaag tgctcggtct gtgctatacg cgccgcctcc tcggcgtagc
      241 ggtacacctc ctcgagaacg ctccgcgaga agcgaacgcg ctgtcccccc gggaggaaca
      301 tctcgatgaa agagtcgctc gcctggtagg gctttagcgg cgacgatatt cgttctatcc
      361 gcgcctggcc gggacgcgcg ttcgcgaaga ggaatcgacc gctcccgatc gtctcttgct
      421 catgaggcag aacgaagagc acctcgacgc cggagtcgat gagctcctcg gtgagaccat
      481 ggcacgcaac accaaggccg ccattcttca tcggcggata atcccacccg aatgtcaaaa
      541 cacgcatgta gaacgacctg cactcggtct tccccaccac gaaagaaaaa acgcgctctg
      601 cgcccgatac ggtcacctgt atggtagcac gcggacgcgt cctatcgacg tcgctctata
      661 tatgaaaagt gcatatctcc ctatttgccg tccttcgagg tctcgtactt cccgagttgc
      721 gcgagaccgt tttgcttcac gtactggaga agggtcttct gggcggcccc cacgttctcc
      781 ggcttcccct gccatgcgac gaggaccggc tcttggatgg cgcgcgagta tgagaacgtg
      841 atgggccacg actgcggtcc gaacgcggcg atcgcctgga ggttctcggt cgcgcgcttc
      901 ggagtctgac ctccggagag gaagacgatg ccgcctacct cgcgcggcac cgagagtttg
      961 aaggtgcgaa gcgtcgccgc ggcgacctcg tccggcgtcg attggtccgg atggcgatcg
     1021 cctgcgagga ccatgctcga cttgaggagg agtcctttga ggtcgacgcg gaactcccga
     1081 agtttcttga agagtatcgt gagcgtgcgc gtcgtcacct cctcggcgac ctcgagagaa
     1141 tgcgtgccgg ggaacatgac ctccggctcg accatcggga cgagacccgc ggcctgtgag
     1201 aggagcgcgt attccgccat tcggtcggcg ttcatctcga gcgccgcatc ggtcggaatg
     1261 gtgtcaccga tcgtgacgac cgcgcgccac ttcgcgaagc gcgcccctat ctcgtagtac
     1321 tccttgaagc gttcgtcgag gccatcgagg ccatgcgtca cgacctcgtc cggaaattgc
     1381 ggaagcggtc gcgttccctt atccaccttg atgcccggca cgataccgcg cgcggtgagg
     1441 acatcgacga acggcacgcc ctggtcggtc gattcgcgaa gcgtcgtgtc gtagaggatg
     1501 atacccgaga ggaactcctc tatgcccggc gcggtgaaga gtagctcacg aaaatcctgc
     1561 cgattgcccg gctgattcgg aagcttcgcg atggcgagcc gcttgcccgc ggtcgaatcg
     1621 ctctcgtcgg cggcgaggat tccctttccc gcttgcatga gctgcgcgac agtcttgtag
     1681 aggttctcgt tctccatgaa cgttcaatgt attttgaaag tgtaccacgc acgcggcgcg
     1741 gcgctttcga cactatgcac aacgcgtact acgagcggat gcgccaaccg atgcggaaga
     1801 ggtagatggt cacgaagccg aggacggcga cgagtccgag gatgaccgcg aggccgacgt
     1861 acgcgttcgc ctcggagaaa ccgatcatcg agtgtcggat gccgtccgcg aagtagaaga
     1921 acgggttcca ccgcgtgatg tgggccataa ccggcgggag catcgagatc gaatagaaga
     1981 tgccgcctaa gtacgtgaga ggcgtgatga cgaacgtatt gagcgccgag agctgttcga
     2041 acccgttcgc ccagaggccg acgaggatgc cggttaaggc gaagatggtc gagatggcga
     2101 cgacgtagaa gaggaagagg aacggcgacg tgatggtgac cgcatggaag agaacgccga
     2161 tcgcgagaat gccgaccgcg accaaggtcg agcggatgac ggcggcggcg gcgaagccga
     2221 agactatctc gaaatacgag aatggcgcga tgagtatctc ctctatggac ttgatgaagc
     2281 ggcccatgta caccgagctc gacgcgtgcg agaacgacgc catgatgatg ttgagcatga
     2341 ggatgcccgg gaagacgaac gtgatgtacg gaacgccggc gatgagcgag atgcgcgagc
     2401 caatgacgtg cccgaatatg aatatgtaga gggtcgcgga gatgaggggc gacacgagcg
     2461 tctggatgac gacgcgaagc atccgcatca tctcgcggcg gatcatcgta tagatgccta
     2521 tccaatttat gccgttcata tcgcttgttt cgttatctcg aggtaccgct cctcgagcga
     2581 gcggccgcct tccacgaact cctcgcgcgt tcccgtcgcg acgagctttc ccttgtttat
     2641 gatggtgatg tgtcgacaga gccgctcgac ctcctcgagg tagtgcgacg tgagaatgat
     2701 cgtcttccct tcctcattca gttccgtgag gtaacgccaa agttcgtgcc gctgttcgac
     2761 gtcgacgccg gcggtcggct cgtcgaggat gaggagcatc gggtcgtgaa tgagggcgcg
     2821 cgcgaggatg acccgacgtt tgagaccgcc cgagagctcg cggaaggcct tcttccgatg
     2881 ctcgaccaag ttgaaccgaa cgaggagcgc ctcgagccgc tcgcgccgaa cgttcgcggg
     2941 aatgccgtag tagccgccga ggaagtcgat gaggtcctcg accgacgcga agaagtcgac
     3001 gttgtactct tgcgacgaaa cgccgatgcg cttacgagcc tccttgtagt cctcgacgac
     3061 gtcgaggcca tcgacgagaa tacggcccga cgtgatcttg ttcacgccga tgatacagcc
     3121 gatcgtgctc gacttccccg caccattggg gccgaggaag ccgtagaaat cgccgcgccg
     3181 tatcgagagg gacacgtcgt cgacggcggt cttctcgccg tacttcttca cgagatgctc
     3241 gatgacgagg atgtcggttt ctgccatcgc ttgagtttac catgtaggcg gcgtatattc
     3301 gcatgaacat gacgagagac gaggaatggc tccttcggga aaagtatgaa ggcgatatga
     3361 acccatcgtt cgtcgaagac tgcgcgcgtc tccgcgccgg cgaaccgctt gcgtacgtca
     3421 tcgggcacgt gccgttcctc ggcgtgaccg tctacctcga ttcgcgcccg ctcattcccc
     3481 gtgccgagac ggagttctgg gtcgagcggg ccatcgcgga gatgcccccc catgaaccca
     3541 tccgcgtgct cgacctctgc tccggttcgg gcgcgatcgg cctcgccgtt ctcgcgaagc
     3601 gcctgaatgc atccgtcgac ctcatcgagc tcgaggagcg ccaccacgcg accatcgagc
     3661 ggaatctccg cgagaacggc gtctccgcta gtcgggcgcg cgtcttaggc ggcgacctct
     3721 tcgagcgagc ggagggcccg tacgaccaca ttctctcgaa cccgccgtat atcgaccccg
     3781 cgctcgaccg cgtggcacag agcgttcgcg cgttcgaacc ggcggaagcg ctctatggcg
     3841 gtgagggcgg cctcgagctc gttcggcgca tcatgaggga ggcggagggg ttcctcgcgc
     3901 cgtatggacg cctctacatc gagcacgaac ccgaacagag cgccgctatc gccacggcgg
     3961 cgaaggaggc agggcttgcg tgccgcacgg agcgcgacca gtacggggtc gaccgctact
     4021 ctgtcctttc ttgtcttgcc cctcgattcc acgtagaatc tagccatgga taaaaaactc
     4081 tccacggaac cctataaggg cacccgcgac ttctacccgg aggatatggc cgtggagcgc
     4141 tacatcttcg acacgtgggc caaggtcgcc gaatcgttcg gctttgagcg ctacgacgcg
     4201 tccatcctcg aaccgtcgga gctctaccgg tcgaagggtg ccgagaacga ggagctcgtg
     4261 aacgagcaga cgtacacgtt cgtcgaccgc ggcgagcgcg aggtaacgct ccgccccgag
     4321 atgacgccga ccgtcgcgcg catggtcgcg ggcaagcgcc gcgaactctc cttccccgtt
     4381 cgctggtact cgatcccgaa cctctttcgc tatgaacgcc cgcaacgcgg acgccttcgc
     4441 gagcactggc aacttaactg cgacatgttc ggcgtcgacg acgtcgcctc ggacatcgag
     4501 atgatcgcgc tcggctaccg catcctcctc gccttcggcg catcgccgaa gatgttcgag
     4561 atacgcgtga acgaccgcac ctggctgaag ctccaactct ctgactacgg tctctccgaa
     4621 gagcaaatga aggcgatgct caccctcatc gacaagcgcg acaagatcga cgatttcgac
     4681 gaggaggccg agaagatcgc cggaaaaaag gtatcgctcg acttcacgac accgaacccc
     4741 gagcatcgcc tcatgcaggt catcgagggt ctcaaggcgc tcggcatcga gaacgtcgtc
     4801 atttcgccct cgacggtccg aggtttcagt tactacaccg gcaccatctt cgagttcttc
     4861 gacaccgcgt ccgagaacaa ccgctcgctc ctcggcggcg gccgctacga taacctcacc
     4921 gcgctcttcg gcggcgaccc ggtctctggc gtcggcttcg gcatgggcga cgtcaccatg
     4981 cgagacttcc tcgagacgca tagtctcctc accaaggacg tgaagacgac cgcaccgacc
     5041 ctcgccatcc ttcccctcga cgccacgcac aatctcgccg cagaggtgct cgcggaggag
     5101 ttccggtccg cgggcgtccg gaccgcggtc gacctctccg accggaaggt cggcaagaag
     5161 ctctccgcgg cgagcgacgc gggcgtggca tacgttatcg tcctcggcga agacgaggcg
     5221 gcgtcgcgct cgtacgccct caagaacctg ggaaccggtg acgagacgaa gggttcggct
     5281 gaagaactcg ctaagcacat cgcacgataa atgcgctacg tcgtattcga cctcgaaacg
     5341 cagaacatct tctccgacgt cggttcgaac gacccgtctg acctcgacgt atcggtcggc
     5401 tcggcgtacg actcgggtac cgacacgtac acgacggtca ccgtcgacga gctccacaaa
     5461 ctctggccca ttttcgaaca ggcggacctc ctcgtcggat acaacagcga ccatttcgat
     5521 atcccgctcc tcaataaata ctatccgggc gatctcacga cgataaagag cgtcgacctc
     5581 tgcagtgcgg tcaaggattc cctcgggaag cgccttcgtc tcgattctct cgcacaggcg
     5641 accttgggct cgaagaaatc cgggaacggt ctcatggcca tcaaatggtg gcgcgaaggc
     5701 gacatcgcct ccataaagaa atactgcgag caggacgtta agatcacgaa gggcatcttc
     5761 gagtacgcgc ttcagaacgg caagcttctc tacaaggacg gccaccggaa gaaggagatc
     5821 cccatcgata cgtcgtcctg gacgccgcac gaggagcact cgatgacgtt ctcactcccg
     5881 ttttgaaaag gcccgcatat gcgggtcttt cactagccga agaaggtgac gaagagaacg
     5941 aagcacgcga gcgcgatcct ataccagatg aagggccaga gcgaatgctt tcgtatgaag
     6001 gagaggagga atcgtatgga aatgagaccg acgacgaacg cgacgatcga gccgacgaac
     6061 accgagaacc acgcgacgtc ctcacccgag gagatgagct cgaggagcat cttgaggccg
     6121 ctgcccacga tgaccggaag accgaggagg aacgagaaac gggcggc
//
LOCUS       DASTSP010000025         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_939462, whole genome shotgun sequence.
ACCESSION   DASTSP010000025 DASTSP010000000
VERSION     DASTSP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939462"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..659
                     /locus_tag="VFS75_01045"
     CDS             138..659
                     /locus_tag="VFS75_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4677286.1"
                     /translation="MKAFDEFSDALFRHAMYRISDRERAVELVHDTFTKVWGYLRDGH
                     TIESYKPFLYKVMNNLIIDEYRRRKEYSLDALMEEEGTDEGSFPELYEGGLEEVTFSL
                     DAQKASDLISELPVSYREVLTLRFVDGLGPKEISALIEESENVVSVRIHRALRALRDM
                     IGKREDEADRRLK"
     gene            662..2047
                     /locus_tag="VFS75_01050"
     CDS             662..2047
                     /locus_tag="VFS75_01050"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEU4677287.1"
                     /translation="MKSFDSELKKYADKVSLKAAERRELRERVLSYMEYHPLPKQAVS
                     VEELRGIPSEAFTVFHFTRFHVRLMGGALALVLILAPLVAERSVPGDVLYLVKTGINE
                     KIQKQLANSPYEKIEFETKLIERRVAEARSLAQEGKLSDDVKNQLAADVKEHSDAVQN
                     NLSELRSMDKDGAAIAEITFNTSLQVQSAVLNADDTVKDTALIEGIKDVVAKAQEQVA
                     TTQEENKPSFGGLMAQVESDTSRAYELFGTVKKSATPEEVGDIDRRLSDIDRLVDEAK
                     EMQQTDEGKAIDDLTKTLGLTQKLIAFMTDIDVHETVALEALVPVVPSLEERTALARR
                     NLESVETRAANVFMKVDGIVDPGKKGKVTGGLHDADALAAEVKSSLDSGNIDAAEAKL
                     AEVEALISDLGVLAEAPASTTGNGTGVGLAEGEGTSTDVGVMSVAATLAPEDTGTTTG
                     TSTATGTPTAR"
     gene            complement(2097..3236)
                     /locus_tag="VFS75_01055"
     CDS             complement(2097..3236)
                     /locus_tag="VFS75_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU4677288.1"
                     /translation="MILLGLMKSAAAAVLIAYLTFTNGIADFIDAHLLHTPTETTAPQ
                     AETELRPLGEGLAERAIPRVLLDHLEYQRAAALASKETEPPAAVPEGESLTDAVEKSL
                     VNIFCQYKTDRFIRTTTGSGFFINRKGVIITNAHVAQFLVLEDAKNLTGNVECVIRTG
                     DPAEPRYTAELLYISPAWIFDNADLITEEAPRGTGERDYALLYVSKSVDGTPLPTSFP
                     AIPVDTALLTRSVAGSDAVTGGYPAEKLFKEGVDAKLEPVIASTTIGTLYTFGSNYAD
                     IFSVSDSPVGEQGASGGPVASLKSDDVIGLIVTKGDEETEGEHSLRAITLSYVDRTMK
                     EETGFSLIDNMQGDLAARGAVFKKAMLPFLADILSSELNTTTRRD"
BASE COUNT          674 a          971 c         1096 g          629 t
ORIGIN      
        1 ttcatatttt tagactgaaa gaagtgggtt ttttggccgt catcgcttat aatgatttcc
       61 acatggcgga gaaccgcaaa aagccaaacg ggaaaccagg aggggcttct cggggtacgt
      121 ccccggagga gcgatttttg aaggcgttcg atgaattctc ggatgctctc tttcggcacg
      181 cgatgtatcg catttcggac cgagagcgag ccgtagagct cgtccacgat acctttacga
      241 aagtgtgggg gtaccttcga gacgggcata cgatcgagtc ctacaagccg ttcctttaca
      301 aggtgatgaa caacctcatc atcgacgaat accgcagacg taaggagtac tctctcgatg
      361 cgctcatgga agaggaggga acagacgaag gttcgttccc cgaactctac gagggcggcc
      421 tcgaagaggt aaccttcagc ctcgacgctc agaaggcgag cgacctcatc tcggagttac
      481 cggtctcgta ccgggaggtg ctcacgcttc ggttcgtcga cggtcttggt cccaaggaga
      541 taagcgcgct catcgaggag tcggagaacg tcgtctcggt gcgcattcac cgggcgctcc
      601 gggcgcttcg ggacatgatc ggaaagcggg aggacgaagc ggacagacgc ctcaagtgac
      661 catgaaatct ttcgattcag aactaaagaa atacgcagac aaggtgagcc tcaaggcagc
      721 cgaacgacgc gaacttcgcg agcgtgttct ctcgtacatg gagtatcacc ccctcccgaa
      781 gcaggccgtg agcgtcgagg aacttcgcgg cattccctcc gaggccttta ccgtcttcca
      841 cttcacgcgc ttccacgttc gtctcatggg cggcgcgctc gcgctcgttc tcatactcgc
      901 accgctcgtt gccgagcgct ccgtcccggg cgatgtgctc tacctcgtga agaccggtat
      961 caatgagaag atacagaagc aactcgcgaa ctccccgtac gagaagatcg agttcgagac
     1021 gaagctcatc gaacgacgcg tagccgaagc ccgctcgctc gcgcaggaag ggaagctctc
     1081 cgatgacgtg aagaaccaac tcgccgcgga cgtcaaagag cacagtgacg cggtccagaa
     1141 caacctaagc gaacttcgct cgatggacaa ggacggtgcg gcgatcgccg agatcacgtt
     1201 caatacgtcg ctccaggtgc agtcggcggt gctcaatgcg gacgataccg tgaaggacac
     1261 cgccctcatc gagggtatca aagacgtggt cgcgaaagcg caagagcagg tcgcgacgac
     1321 gcaggaggag aacaagccgt cgttcggcgg gctcatggcc caggtagagt ccgatacgtc
     1381 gcgcgcgtac gaactcttcg gaaccgtgaa gaagtcggcg acgcctgagg aggtcggtga
     1441 catcgaccgt cggctctccg atatcgaccg tctcgtcgac gaggcgaagg agatgcaaca
     1501 gaccgacgag gggaaggcga tcgacgacct tacgaagacc ttgggcctca cgcagaagct
     1561 catcgcgttc atgaccgaca tcgacgtcca cgagaccgtc gcccttgagg cgctcgttcc
     1621 cgtcgtaccg agcctcgaag agcgtacggc gctcgcgcgg cggaacctcg agagcgtcga
     1681 gacgcgcgcc gcgaacgtgt tcatgaaggt cgacggcatc gtcgaccccg gcaagaaagg
     1741 gaaggtgacc ggaggtctcc atgacgccga cgcgctcgct gccgaagtga agtcgtcgct
     1801 cgatagcggc aatatcgacg ccgccgaggc gaagctcgcg gaggtcgagg cgctcatctc
     1861 cgacctcggc gtcctcgccg aggcgcccgc gtcgacgacg gggaacggca cgggggtcgg
     1921 gctcgcggaa ggcgagggga cttcgaccga tgtcggcgtc atgtccgtcg ccgcgacgct
     1981 cgcccccgaa gatacgggaa cgacgacggg gacgtcgact gcgaccggga cgcccacggc
     2041 acggtaacag tatgcatagt aagaaagacc ggcactcgcc ggtctttttc gttctcctaa
     2101 tcgcgccgtg tcgtcgtatt gagctccgag gagaggatat cggcgaggaa ggggagcatc
     2161 gccttcttga agaccgcacc acgcgccgcg aggtctccct gcatgttgtc gatgagcgag
     2221 aatccggttt cctctttcat ggtgcggtcg acgtacgaga gcgttatggc gcgaaggcta
     2281 tgctcgccct cggtctcctc gtcgcctttc gtcacgatga gcccgatgac gtcgtcgctc
     2341 ttcagtgacg cgacgggacc gcccgaagcg ccctgctcgc cgacgggcga gtcggagacg
     2401 gagaagatgt cggcgtagtt gctcccgaac gtgtacaagg tgccgatggt cgtcgatgcg
     2461 atgaccggtt cgagtttggc gtcgacaccc tctttgaaga gcttctccgc ggggtaaccg
     2521 cccgtcacgg catcagaacc ggcgacgctt cgcgtgagga gcgcggtgtc gacggggatc
     2581 gcggggaagg acgtcgggag gggcgtgccg tcgaccgact tcgagacgta gagcagtgcg
     2641 tagtctcgct caccggtgcc gcgcggcgct tcctcggtaa tgaggtcggc gttgtcaaat
     2701 atccacgccg gggagatgta gaggagctcc gccgtgtacc ggggctccgc ggggtcgccg
     2761 gttcgtatga cgcactcgac gttcccggtg aggttcttcg cgtcctcgag gacgaggaac
     2821 tgcgcgacgt gcgcgttcgt gatgatgacg ccttttcggt tgatgaagaa accgcttccc
     2881 gtcgtcgtcc gtatgaaacg gtccgttttg tactgacaga agatgttgac gagcgacttc
     2941 tcgacggcat cggtgagcga ctcgccctcg ggtacggcgg ccggcggctc cgtctccttg
     3001 ctcgcgagcg cggcggcccg ctggtattcg aggtggtcga ggaggacgcg cggtatcgca
     3061 cgctcggcga gaccctcccc gagcggtcgg agctcggtct ccgcttgtgg agcggtcgtc
     3121 tccgtcggtg tgtggagcag gtgcgcgtcg atgaagtccg cgatgccgtt cgtgaaggtg
     3181 aggtatgcga tgagtacggc cgcggccgcg gatttcatga gtccgaggag gatcatacgg
     3241 tgattgtatc acgcgattgt gccgcggcgt tccttcgtgc tagaatcctg cggacacgga
     3301 tgcgggcata gtttagtggt aaaatgactg ccttccaagc agtagatgga ggttcgattc
     3361 ctcttgcccg
//
LOCUS       DASTSP010000026         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1834427, whole genome shotgun sequence.
ACCESSION   DASTSP010000026 DASTSP010000000
VERSION     DASTSP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834427"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..2001)
                     /locus_tag="VFS75_01060"
     CDS             complement(94..2001)
                     /locus_tag="VFS75_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU4677289.1"
                     /translation="MQFDPEKVTYFARTDARGKNVPFGIKAKDRQRHVYVIGKTGMGK
                     STLLENMAVQDIQNGEGMAFIDPHGSAAETLLNYIPQDRVNDVLYFAPFDTEHPVSFN
                     IMEDIGADKRHLVVSGLMSVFEKIWVDAWSARMAYILQNTLMALLEFPGATLLGVNRM
                     LSDKDYRKLVVDNVKDPGVKAFWVDEFAKYNERYMQEAGDAIKNKIGQFTANPVIRNI
                     IGQPKSSFDIRKMMDEKKILIMNLSKGLIGETNANLLGSMLTTRIYLAAMSRADLPPE
                     KMREMPNFYFYVDEFQSFANATFANILSEARKYHLNLTIAHQYIEQMEEEVRNAVFGN
                     VGTIISFRVGPFDAEVLETIFSPQFTATDIVNLAFAQVYLTLMIDGIGSQPFSATTLP
                     PIAPPTISCKDMVVAASREKFTKVRADVEVAIRKFHEPVNPPAPKERAEARPAPREGM
                     QTKPQGERQGGERPRQSAPERERQNAERPREQREVPKSDAKPVESKPAEAPKESHRQQ
                     HPKPHRDGPHDRDRRHVDEKQHRGNVDELRSVLKKIVSTAPSDVPAERRNVAKSEELK
                     EALSVAMGEPKPAPKEEPKPAPQTSPAEEATSAPKAAPTPEPPRPKQKGVTPKELERM
                     MRLTTEDKPPL"
     gene            complement(2064..2312)
                     /locus_tag="VFS75_01065"
     CDS             complement(2064..2312)
                     /locus_tag="VFS75_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677290.1"
                     /translation="MSKESFVLLIGIIVFFGPFLGLPTEYKDILFAVAGLLLIVTGYR
                     LRRRAYLASLEREGGERVADAFVESGSTLEPREAFDPS"
     gene            complement(2354..>2686)
                     /locus_tag="VFS75_01070"
     CDS             complement(2354..>2686)
                     /locus_tag="VFS75_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4677291.1"
                     /translation="VTAAFSIAGIPGNAFTFLGFAPQKKGRATFFKRLAAYEVPVIFY
                     ESPHRIAKALESLAAELPTDFRVTVARELTKLHEEVIAGSPAEVRDYFTEHPDRVRGE
                     FVIIVSRG"
BASE COUNT          442 a          784 c          880 g          580 t
ORIGIN      
        1 catccatgcg acctcgttta tccgcacctg tgccgatgcg acggagggcg cgattatgag
       61 gagcgtgagc acgaggcgtg cgagtttcat ggttcaaagt ggcggtttgt cctcggtcgt
      121 gaggcgcatc atccgctcga gttcctttgg tgttacgccc ttttgtttcg gacgtggtgg
      181 ctctggcgtg ggggcggcct tcggagccga tgtcgcttcc tctgcaggcg acgtttgcgg
      241 cgcgggcttt ggttcttcct tcggcgcggg cttcggctcc cccatcgcga ccgagagtgc
      301 ttccttcagt tcctcgctct tcgcgacgtt acggcgttcg gcgggtacgt cgctcggtgc
      361 ggtcgatacg atcttcttca aaacggagcg gagctcgtcg acgttcccac ggtgctgctt
      421 ctcgtcgacg tgccgacgat cgcggtcgtg cggtccgtca cggtgcggct tcggatgctg
      481 ctgtcgatga gactccttcg gtgcctccgc cggcttcgat tctacgggct tcgcatcgct
      541 cttcggaacc tccctttgtt ctcgtggccg ctcggcgttt tggcgctcgc gttcgggtgc
      601 gctctgacgt ggacgctccc ctccctgtcg ctctccctgc ggcttcgtct gcatgccctc
      661 gcgcggtgcg gggcgcgcct ccgcccgctc tttcggagcg ggcgggttga ccggctcgtg
      721 gaacttccga atggcgacct cgacgtcggc gcgcaccttc gtgaacttct cacggctcgc
      781 cgcgacgacc atgtccttgc aggagatggt aggcggcgca atgggcggaa gcgtcgtcgc
      841 ggagaacggc tgtgacccta tgccgtcgat catcaaggtg aggtacacct gcgcgaaggc
      901 gaggttcacg atatcggtcg cggtgaactg cggactgaag atggtctcga ggacctcggc
      961 gtcgaacggt ccgacgcgga aggagatgat ggtgccgacg ttcccgaaga ccgcgtttct
     1021 gacctcctcc tccatctgtt cgatgtactg gtgcgcgatg gtgaggttga ggtggtactt
     1081 acgcgcctcg gagaggatgt tcgcgaaggt cgcgttcgcg aaggactgga actcgtcgac
     1141 gtagaagtag aagttcggca tctcgcgcat cttctcgggc ggtaggtcgg cgcggctcat
     1201 cgcggcgagg tagatgcgcg tcgtgagcat cgagccgagg agattcgcgt tcgtctcgcc
     1261 gatgagaccc ttcgagaggt tcatgatgag tatcttcttc tcgtccatca tcttccggat
     1321 gtcgaacgat gatttcggct ggccgatgat gttcctaatg acggggttcg cggtgaactg
     1381 accgatcttg ttcttgatgg cgtcgcctgc ctcctgcatg taccgctcgt tgtacttcgc
     1441 gaactcgtcg acccagaacg ccttcacgcc cgggtccttc acgttgtcga cgacgagctt
     1501 ccggtagtcc ttgtcggaga gcatacggtt gacgccgagg agcgtcgcgc ccgggaactc
     1561 gaggagcgcc atcaaggtgt tctgaaggat gtacgccatg cgcgcgctcc acgcgtccac
     1621 ccatatcttc tcgaagacgg acatgaggcc ggagacgacg aggtgccgct tgtctgcgcc
     1681 gatatcctcc atgatgttga aggatacggg atgttccgtg tcgaacggcg cgaagtagag
     1741 gacgtcgttc acccgatctt ggggaatgta gttgaggagc gtctccgctg ccgagccgtg
     1801 cgggtcgatg aaggccatac cctcgccgtt ttggatgtct tgaacggcca tgttctcgag
     1861 aagcgtcgac ttccccatac ccgtcttgcc gatgacgtac acgtgccgtt ggcggtcctt
     1921 cgccttgatg ccgaagggaa cgttctttcc ccgggcgtcg gtccgcgcga agtaggttac
     1981 tttctccgga tcgaactgca tacgcagtaa gtatagcaaa caaaaagccc cgggccgtga
     2041 aggcccgggg tgcagtacgt gcgctacgac ggatcgaacg cttccctcgg ctcgagcgtg
     2101 cttccgctct cgacgaacgc atcggcgacg cgctcgccgc cctcccgctc gagagacgcg
     2161 aggtacgcgc ggcgacgaag gcggtacccc gtcacgatga ggaggagtcc ggcgaccgcg
     2221 aagaggatgt ccttgtattc cgtggggagc cccaagaagg gaccgaagaa aacgatgata
     2281 ccgatgagga ggacgaagga ttccttagac atgcgtagag tataccgtgt aagcttgaat
     2341 tgaaaaagcg ttgtcaaccg cgcgagacga tgatgacgaa ctcgccacgg acgcggtcgg
     2401 ggtgctcggt gaaataatcc cgaacttctg cgggcgatcc tgcgatgacc tcctcgtgga
     2461 gcttcgtgag ttcacgggcg accgtgacgc ggaagtccgt ggggagctcg gccgcgagcg
     2521 attcgagcgc cttcgcgatg cggtgcggcg actcgtagaa gatgaccggt acctcgtacg
     2581 ccgcgaggcg cttgaagaac gtcgcgcggc ccttcttctg cggtgcgaag ccgaggaacg
     2641 tgaacgcgtt ccccggaatg ccggcgatcg agaacgcggc ggtcac
//
LOCUS       DASTSP010000027         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1165648, whole genome shotgun sequence.
ACCESSION   DASTSP010000027 DASTSP010000000
VERSION     DASTSP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165648"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="mltG"
                     /locus_tag="VFS75_01075"
     CDS             <1..900
                     /gene="mltG"
                     /locus_tag="VFS75_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU4677292.1"
                     /translation="LTLFVVLSSRYLERPSSAFPVGEEIVVTEGAPQSDITDMLAARH
                     IVRSSLYLYLYLRQHYPDEFIKAGTYRFEDPLRTSEVAEALISGKDSTPLLKVTFPEG
                     FRADRLLSYLPESLRASTTVNLAQFEGYLFPDTYFITAGTSVNDIITLLTDTFRQKVS
                     PLAGDIAKSGYTEREVVTLASIVEREAKDARSKRIVAGILENRLAEGMPLQVDAAFDY
                     LLGKESSELTDEDLTLDSPYNTYKYAGLPPTPISNPGLDAIEAVLQPERTDYLYYLTD
                     ADGNFHYAKTFEEHKANKQRYLK"
     gene            933..1994
                     /gene="mnmA"
                     /locus_tag="VFS75_01080"
     CDS             933..1994
                     /gene="mnmA"
                     /locus_tag="VFS75_01080"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU4677293.1"
                     /translation="MSKGTVFVGLSGGVDSSVSALRLLKAGYDVVGVFMMTWLPEFLD
                     CDIEAERLDAMRVAATLGIPFRTFDAVEAYREGVANYMIREYEAGRTPNPDVMCNRRV
                     KFGAFFEYAMGEGADYVATGHYARVVKRDSYELHRGKDTEKDQSYFLWTLTERELART
                     LFPVGGSEKRVVRADAARYSLPTAGKRDSQGICFLGQVDIREFLSHYVPVRPGDVLNE
                     RGTVIGRHDGALFYTIGQRTGFTVRTAGREERAHYVVEKNMERNTLTVSDRPRPVSGD
                     RIALSDVNVIGGVLPERAFAEFRYRQRPFPVSLEEAAGDHAMLSVTEAGIDLPSPGQS
                     CVLYDGTRCLGGGIIDRIV"
     gene            1991..2821
                     /locus_tag="VFS75_01085"
     CDS             1991..2821
                     /locus_tag="VFS75_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEU4677294.1"
                     /translation="MTIITKADGNTEEFKPGKLVSSLKKAGAKPAEVSRIVAAIESGL
                     KDGMRTQIIYQKAFQLLRESNDPVAAKYSLRRAIFGLGPSGFPFEDFLGRIFEAEGYK
                     TKRRLTIRGKCAVHEIDLAAYSPEHTFIAEAKFHMQPGTKSDLQVAMYSYARYLDLQS
                     ARVCKDDVCGIIDLFLITNTKFTQAAIKYGKCVGINMLSWDYPKDDTLQEKIERHKLY
                     PITVLSRLPAGYKVVLLERGVILCTELLRRAALLQEIGLSGTKIDAIFNEINALYGHK
                     "
     gene            2933..>3231
                     /locus_tag="VFS75_01090"
     CDS             2933..>3231
                     /locus_tag="VFS75_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677295.1"
                     /translation="MADTVERDDSRKTVVAFITGLLIGGLLVWVFSSTPANENKTVPT
                     APEAPSAPSTTGSKDTSSNGNGSSTDTTSNGSASGDVVSDQGAGNSVELGSIDFPT"
BASE COUNT          681 a         1024 c          936 g          590 t
ORIGIN      
        1 ctcacgctct tcgtcgtcct ctcctcgcgc taccttgagc gaccgtcgtc tgcgtttccg
       61 gtcggtgagg agatagtcgt gaccgaaggg gcgccacaga gcgacatcac cgacatgctc
      121 gccgcgcgcc acattgttcg ctcgtctctc tatctctacc tctacctgcg tcagcactat
      181 ccagacgagt tcatcaaggc ggggacatac cgcttcgaag acccacttcg aacgagcgag
      241 gtcgccgagg cgctcatctc gggaaaggac agtacgcccc tcctcaaggt gacgttcccc
      301 gagggtttcc gcgccgaccg cctcctttcg tacctcccgg agtcgctccg tgcctcgacg
      361 acggtcaacc tcgcgcagtt cgagggctac ctctttcccg acacgtactt cataacggcg
      421 ggaacgagcg tgaacgacat catcacgctt ctcacggaca catttaggca aaaggtctcg
      481 ccgctcgccg gcgatatcgc gaagagcgga tacaccgagc gggaggtcgt caccctcgcg
      541 tccatcgtcg aacgcgaggc taaggacgcg cgctcgaaac gcatcgtcgc gggcattctc
      601 gagaaccgcc tcgcggaggg tatgccgctt caagtcgacg ccgcgttcga ttacctcctc
      661 ggtaaggaga gcagcgagct caccgacgag gacctcacgc tcgattcacc ctacaacacc
      721 tacaaatacg ccggccttcc tccgacgccc atctcgaatc cgggactcga tgcgatcgag
      781 gccgtgctac agcccgagcg taccgactac ctctactacc tcaccgacgc cgacggcaat
      841 ttccactacg cgaagacgtt cgaggagcac aaggcgaaca aacagcggta cttgaagtag
      901 gcgcgattct gcgtctgtgc tatggttgcc gcatgagcaa aggaacggta ttcgtcggac
      961 tctcgggagg cgtcgattcg agcgtctccg cgctccggct ccttaaggcc ggctatgacg
     1021 tcgtcggtgt cttcatgatg acgtggttac cggaattcct cgactgcgac atcgaggccg
     1081 agcgcctcga cgcgatgcgc gttgccgcca cgctcggtat cccgttccga acgttcgacg
     1141 cggtcgaagc gtaccgtgag ggcgtcgcga actacatgat ccgcgagtac gaggccggac
     1201 gaacgccgaa tcccgacgtc atgtgcaacc gccgcgtgaa gttcggcgcc ttcttcgagt
     1261 acgcgatggg ggagggagcc gactacgtcg cgacgggaca ctacgcgcgc gtcgtgaaaa
     1321 gggactccta cgagctccac cggggaaagg ataccgagaa agaccagagc tactttctct
     1381 ggacgcttac ggagcgcgag ctcgcgcgaa cgctcttccc cgtaggcggg agcgagaagc
     1441 gcgtggtacg ggccgacgcg gcgcgatatt ccttgccaac ggcaggcaaa cgtgatagcc
     1501 agggcatctg tttcctcgga caggtcgaca tccgcgaatt cctctcccat tacgtccccg
     1561 tacgaccggg cgacgtcctc aatgagcgcg gcaccgtcat cgggcgacac gacggcgcgc
     1621 tcttctacac gatcggtcag cgcacgggct tcacggtgcg aacggcgggc cgcgaggagc
     1681 gcgcgcacta cgtcgtcgaa aagaacatgg agcgaaatac gctcaccgtg agcgaccgtc
     1741 cgcgacccgt ttccggagac cgtatcgcgc tctcggacgt gaacgtgatc ggtggagtgc
     1801 tccccgagcg tgcgttcgcc gagtttcggt atcgccagcg gccattcccc gtctcgctcg
     1861 aagaggcggc gggtgaccat gctatgctat cggtgaccga agcgggcatc gacctgccgt
     1921 cgccgggaca gtcatgcgtc ctctacgacg gaacgaggtg cctcggcggc ggcataatcg
     1981 atcgaatcgt atgaccatca tcacgaaggc cgacggcaat accgaggagt tcaagccggg
     2041 aaagctcgtc tcgtccctca agaaggccgg cgcgaagccc gctgaagtgt cgcgcatcgt
     2101 cgcggccata gaatcgggtc tcaaggacgg gatgcgcacg cagatcatct accagaaggc
     2161 gttccagctc ctccgagaat cgaacgaccc cgtcgccgcg aagtactcgc ttcgccgcgc
     2221 catcttcggc ctcggcccgt cgggtttccc gttcgaggac ttcctcggaa ggatattcga
     2281 agccgaaggc tataagacga aacgccgtct caccatccgc ggcaagtgcg cggttcacga
     2341 gatcgacctc gccgcgtact ctcccgagca cacgttcatc gccgaggcga aattccatat
     2401 gcagccggga acgaagtcag acctccaggt agccatgtac tcgtacgcgc gctacctcga
     2461 cctccagtca gcgcgcgtct gcaaagacga cgtctgcggc atcatcgacc tcttcctcat
     2521 aacgaacacg aagttcacgc aggccgccat caagtacggg aagtgcgtcg gcatcaacat
     2581 gctctcgtgg gattatccga aggacgacac gctccaggag aagatcgagc gtcacaaact
     2641 ctatccgata accgtactct cccgccttcc cgcaggctac aaggtagtgc tcctcgagcg
     2701 gggcgtcatt ctctgcacgg aacttctccg gcgcgccgcg ctcctccagg aaatagggct
     2761 ttctggcacc aaaatagacg ccattttcaa cgagataaac gcattgtacg gccataaata
     2821 ggtcgttttg acaaaggaaa ggcggaaatg cggagttctg gtctccgtac aacgccattt
     2881 tcgcccatgc tataatcctc acgattacca gggtagttta cgtaagcaaa gtatggcaga
     2941 tacagtagaa agagatgaca gcaggaagac tgtcgtcgcg tttatcacgg gactcttgat
     3001 cggcggattg cttgtgtggg tattctcatc gactccggct aacgagaaca aaacggtacc
     3061 gacggctccg gaagcgccga gcgctccgtc gacgaccggt tcgaaggaca cgtcgtcgaa
     3121 cggcaatggc tcgtcgactg acacgacatc gaacgggagc gcgagcggtg acgtagtgtc
     3181 cgatcaggga gcgggcaact cggtcgaact cggttcgatc gatttcccga c
//
LOCUS       DASTSP010000028        14656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_273719, whole genome shotgun sequence.
ACCESSION   DASTSP010000028 DASTSP010000000
VERSION     DASTSP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273719"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /gene="ligA"
                     /locus_tag="VFS75_01095"
     CDS             complement(<1..1464)
                     /gene="ligA"
                     /locus_tag="VFS75_01095"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU4677296.1"
                     /translation="MAPKPTERRAKELARLIAHHQKLYHEKDAPEISDEAYDSLVREL
                     RGITEAHPDLAHLYEATEVVGGKPSEAFAKVRHRVRQWSFDNVFSDAELRAWEERLMR
                     ALEKQGMSERVSYVSEHKIDGLKMVLEYEKGALVRAATRGDGVVGEDVTHSARMIADV
                     PTELAKPVTLTAVGEVWLSRREFERINRERERSGEPLFANPRNAAAGSVRQLDPTVTK
                     GRKLSFFAYDVDYVDERTLDGKSVPTTQYEELAFLRSLGFTVNPHAKHCATLDDAIAD
                     YRRWAPKKLSMHYGMDGTVIKVDDIRLQNALGYTAKAPRFGIAYKFPSEEATTVLEDI
                     VLQVGRTGVVTPVAHLRPVLIAGSTVSRATLHNEDQIKRLDARIGDTVVLRKAGDVIP
                     EIVSVVLALRPKGAKPYTFPKKVAECGGDGSIERIPGEAAYRCVARDSAVLHRRRLYY
                     FVSRGAMNVDGVGPKIIDLLLDETLINTASDLYTLTEG"
     gene            complement(1546..2175)
                     /locus_tag="VFS75_01100"
     CDS             complement(1546..2175)
                     /locus_tag="VFS75_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677297.1"
                     /translation="MKVNRTVPTDERGITLLITLLLMGVLLGVSTSLINITLKQYQLS
                     GIALASEIAFQAANAGLECALYHDFPAAGVSPFAVPDDGSEQAVVPNIDCMGVNASSD
                     DGSNLPNYSDANETDGKSMSGGEQRFEFTWGSDPDAADKLCSIVSVYKFSSGGSDVPV
                     TIGNRDFRGGDPCPAGSVCTVIQARGYNVPCNQISTSPRVVEREYTQVY"
     gene            complement(2165..2740)
                     /locus_tag="VFS75_01105"
     CDS             complement(2165..2740)
                     /locus_tag="VFS75_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4677298.1"
                     /translation="MIYRATQHKSVIHGFTLVETLVAITVLLLVIIGPITVAQKGIRA
                     AYYANEQVTAVFLAQEAVEAIRELRDDAALAAYDNPGGGDTWNWIGALPPDCTDGTGC
                     AFDITSHAFESCALDNNCELKVTAAGAYNYEAGGTDSGYVRRVYVTAAAPDAIDVTVS
                     VTWTSTTFGGGTPRTITLETWIYDHYARYES"
     gene            complement(2737..3336)
                     /locus_tag="VFS75_01110"
     CDS             complement(2737..3336)
                     /locus_tag="VFS75_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4677299.1"
                     /translation="MSTRITKQQREKGFTLVELMVSLTVFSIVMTISVGTLLVLIDLN
                     SKAQAVYSATTNLSFALDSMTREIRTGYRYYCKESSGNPEEALPDSTVTNDCDAGDFV
                     SFIREEDDVRMGYRLDEATHAIEQKESSGNWVPITADDVSVDEFSVTVDNSTTYSPGN
                     DEEQPTADIVVKGHVNNGLDTDTDFYIQTHIVQRRLDII"
     gene            complement(3341..3958)
                     /locus_tag="VFS75_01115"
     CDS             complement(3341..3958)
                     /locus_tag="VFS75_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4677300.1"
                     /translation="MLTSSTSRTSSSSPRGFTLVELLVSVAIIGIVTSIVLVKYNSFD
                     SNVLLKSAAYEMALNLREAQVRSVSVARNETSFDYPFGVSVPVGKTYQSFRYLVTDEG
                     DPSYKEFPVYGEDTEDIRTFFLDRSMVVGDLCIDQNGTETCGLSRLDISFRRPKFKGL
                     FHVDGFTQVEDASIDSGTVKLTSATPGSVYAVKVTNLGQISVYKE"
     gene            complement(3915..4736)
                     /locus_tag="VFS75_01120"
     CDS             complement(3915..4736)
                     /locus_tag="VFS75_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU4677301.1"
                     /translation="MATYLTVMFFVLGAVVGSFLNVVIYRFHTGKSLGGRSHCLSCGK
                     TLSWSELLPVVSYLAQRGICRECGAYIPPRYLVVEVLTGLSFTWVWHLFANAPVLLVF
                     YLVLVSILVVIVFYDIRHTIIPNELTAAVLALALVFTFFDAFTRHDVAVVLSRLIAGA
                     SAGFFFYGLWYVSRGRWIGFGDAKLAVPLGMMVGLGGVFSMLVFSFWVGAVITLALLG
                     LERLLRRGKARLQFLGAPLTIKSEVPFAPFLVLGFLIVQFFHADIFDITYLVFFP"
     gene            complement(4740..5225)
                     /locus_tag="VFS75_01125"
     CDS             complement(4740..5225)
                     /locus_tag="VFS75_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4677302.1"
                     /translation="MHSPHPRSSAAIRGFTLLELVVVIAIIAILAAVVIGATSQQKNA
                     GGNAGVEQNLVNARSQIALYYVNNNNSYLNACADARIRKMVQAAAKAANITPSAAAYA
                     NTDVGDWNSEACHATAAAWAIWVPLRSSTSASPAAWCVDSTNASRQVTGSLASGAVAC
                     P"
     gene            complement(5334..5771)
                     /locus_tag="VFS75_01130"
     CDS             complement(5334..5771)
                     /locus_tag="VFS75_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4677303.1"
                     /translation="MMTNLYKRGFTLIELLVVIAIIGILASVVLASLNSARDKGADAA
                     IKSSINNARAQAELFYTNNSNSYSTVCSSGTNNISQMMTGATNAGSGSVVCADGGAGT
                     AWGAAAQLKSNTSLWYCVDSTGNSTTTAVANGGLTDTSDWTCG"
     gene            complement(5882..7093)
                     /locus_tag="VFS75_01135"
     CDS             complement(5882..7093)
                     /locus_tag="VFS75_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU4677304.1"
                     /translation="MLFSYKAVDKLGTPREGTVDAQNVEAAIDTVEGRGYTIITVDPV
                     EGGKSSILDFEVTWFDRVSNKDIVILSRQIATLFEAQVSALRVFRLLGSEVENQKLQR
                     ILTEVSDAIQGGSSISRALAQHPDVFSQFYVSMVKSGEESGTLDKTFMYLADYLDRMY
                     LVVSKARNALIYPAFVISIFVGVMTLMLTLVIPSISQILIDSGQEIPIYTRIVMGISS
                     ILTDYFGLILVFLTLVGVVFWQFQKTEAGKYMIDEFKLAVPFVGSLYEKLFLTRICDN
                     LSTMLGSGISMVQALEVTAEVVDNRVYREIIDNTLVDVKAGKSFADAIAEYPEIPGVL
                     AQMSKVGEETGKLGDILNTLSVFYRREVNNTIDTLISLIEPLMIVVLGLGVGTLLASV
                     LIPIYNITGGI"
     gene            complement(7098..8168)
                     /locus_tag="VFS75_01140"
     CDS             complement(7098..8168)
                     /locus_tag="VFS75_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU4677305.1"
                     /translation="MVSVDYKRELDDLIDIVTSEKGSDIHLSVGSHPIIRVDGSLVPL
                     AKKPVLTNVDVAGFVSVLLTKEQEQRFLESREIDFSFSTREGTRFRGNGFFQRGSMSI
                     ALRYISNVIKTFEELNLPPVLESFTQRPQGFFLVVGPVGQGKSTTLAAMIEHINQNRA
                     EHIVTIEDPIEYIFEPKKSLIDQREVRVDTADFHVGLHSAFRQDVDVIMVGEMRGQDT
                     ISTAVTAAETGHLVFSTLHTNNAAQTIDRIIDSFPSTQQDQIRVQLAGSLTGIFSQRL
                     IPRISGSLVPAYELLISNAAVANLIRERRTHEIQTVIETGMEQGMIDMNRSIVSLVQK
                     GEITVENAFAYSTNPKGLERLM"
     gene            complement(8180..8545)
                     /locus_tag="VFS75_01145"
     CDS             complement(8180..8545)
                     /locus_tag="VFS75_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4677306.1"
                     /translation="MNILLVDDDAFLRDMYATKFRELGHTIDAAQTAENALAKLESGT
                     YDVILMDMVMPGMNGIELLKEIKSKNLGGNPTCIMLSNQSDDADKEAALGNGAVGYIV
                     KAEFIPSEVVEQVLKLVAK"
     gene            complement(8579..10366)
                     /locus_tag="VFS75_01150"
     CDS             complement(8579..10366)
                     /locus_tag="VFS75_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU4677307.1"
                     /translation="MNLLALLAEKNVINAALEERVSARAAEGGSYEKALIDEGVDPDI
                     IRKTIGEYYELPIRTITETQRIDSAVLKYLSEESARHYGILPIAFEDDVLIVGITDPE
                     NLSYRDALNFITMKDQIPYKLVFILERDFENGMKSYENLTGEVDEALGVLESELKKEE
                     DKATEVAGKVEHIKEDAPVTKIVATILRYAVDGGASDIHIEPRDQKTIIRFRVDGVLV
                     HSLELPQKVHPAVVARIKILASLRLDEKRKPQDGRFSASIDGRPIDFRVSILPTNHGE
                     KVVMRILDTDAGVRTLEDTGVSDYNIELIRRAIQQPYGIILISGPTGSGKSTTLYAML
                     SEVDRIGKNVISLEDPVEYNIDGVSQSQIRPEIGYTFANGLRTALRQDPDVIMVGEIR
                     DKETAQLAIQAALTGHLVLSTIHTNNAVGVIPRLIDMGVDPYLIAPTLELALAQRLVR
                     RICPGTGKKTPVTGSLKVMIEKEFSDLPAEFRKIIPKAEDVLFAEPSAECSSGTKGRV
                     AVMEAIPVSESIEKLILDGGDESEILKAARANGFISMKEDAIVKALNHVIPFEEVSTL
                     GGELLVSDEAEKEKEAMHAPAPEAPVDNP"
     gene            complement(10386..10679)
                     /locus_tag="VFS75_01155"
     CDS             complement(10386..10679)
                     /locus_tag="VFS75_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677308.1"
                     /translation="MSHLLRNLLFALIATVVVGVLYKTFFMGGSTDLTAISSEKHDVV
                     LQTEKILADIQKINTYTIDDSVFDDARFTSLQSYRVPITDVGTGRSNPFAPVQ"
     gene            complement(10688..11254)
                     /locus_tag="VFS75_01160"
     CDS             complement(10688..11254)
                     /locus_tag="VFS75_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677309.1"
                     /translation="MMRTTTPILSILIAVLLFVFFTQPQYLKAKDLMNQIDEYHQATK
                     TYADFTGALENKLAKKSDRSPYEMERLDQLVPEEIDDTQDLVDLEALAQKNNLLFQVT
                     EVDDKQLSFVDRSGGGASSAATSRDELTTVDISFAVVGTYDQFKSFLKDLEKSLTIYE
                     VTQLSLTVAEGPFQQFKMTVRTYALPNK"
     gene            complement(11251..12036)
                     /locus_tag="VFS75_01165"
     CDS             complement(11251..12036)
                     /locus_tag="VFS75_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677310.1"
                     /translation="MAYIPRSSLIPNETASAIPVQMKKRRTIHAFGLAATLIFLLSLF
                     CAVGIFLYKGVLEKRLEDAKQSLGAVSDGGNQKKIDEIRLYNDRLRVARSLLDNHVAT
                     SRIFEEIENSTKKSVQYRSLEFVNDPGFEATLTLTGDTKEFSSIALQKMQILEDSLFS
                     DFVLQDITTSKTAKAQGETATAPQAGISFKVVGVFKKDLISYGDDVPASEPAPQETAP
                     SSAPEDVAPAVEADTSSEPATAPADASTTPVDAPETPDSQTQS"
     gene            complement(12094..13191)
                     /gene="pilM"
                     /locus_tag="VFS75_01170"
     CDS             complement(12094..13191)
                     /gene="pilM"
                     /locus_tag="VFS75_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEU4677311.1"
                     /translation="MAAFDLSSLKSFLKPQRTAATSAKGGVIGIDIGSSAIKLIQLKD
                     ERGVPTLETYGELQLGPYEGIDLGRGTHLTPVKTIEALVDILRESGATATKAAFAFAY
                     NASFITIASVPTADPERLASMIPIEAKKYIPISLTKVSLDWFPLTPHEEEGRTDVLIS
                     AIYNDTLERYQSIMHGSGLTPVASEIEIFSSIRSVLSPKDTTVAILDCGASATRLYVV
                     RNGVVEKAHSVPLSGVELTDLIAKTEGIEFKDAEELKRTVGMGGGDGNAALQKTITGS
                     VERSFREVHTVMSRYEETSHAKIDKVVLSGSGSLLTGLRPYLEDMFSRPVILSDPFSK
                     VAYPAFLEDTLRLAGPSFAVAVGVALRVYRK"
     gene            13232..13828
                     /locus_tag="VFS75_01175"
     CDS             13232..13828
                     /locus_tag="VFS75_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU4677312.1"
                     /translation="MVHFLTSVIDALFPPEGDALVVRELTGREVSLLFEPRVVDGVVA
                     LTRFSDARVRALIHEAKFHGNAAATRHLGSLLARYLEHASYDIVVPVPLSRRRFMERG
                     YNQVARIIAGSEVSGDRVVSSALSRMRHTRPQTDLEKKARAANVSGAFAVAHADALRD
                     KRVILVDDVTTTGATLAAAREALLAASPRSITCVALAH"
     gene            13972..14058
                     /locus_tag="VFS75_01180"
     tRNA            13972..14058
                     /locus_tag="VFS75_01180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14006..14008,aa:Ser,seq:gct)
     gene            14392..>14656
                     /locus_tag="VFS75_01185"
     CDS             14392..>14656
                     /locus_tag="VFS75_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEU4677313.1"
                     /translation="MKSHPFVLPSKKAAGRKNTIKSFKAKADARRTTTEVIADFLTRS
                     FGTVSFLVLNAIFFIVWIAWNMGLTPFPVMDPFPFGLLTMVVSL"
BASE COUNT         2717 a         4270 c         4693 g         2976 t
ORIGIN      
        1 gccctcggtg agcgtgtaga ggtcggaggc cgtgttgatc aaggtctcat cgaggaggag
       61 gtcgatgatc ttcggtccca cgccgtccac gttcatcgcg ccgcgcgaga cgaagtagta
      121 gagccggcga cgatggagga ccgcggagtc ccgcgcgaca cagcggtacg cagcctcgcc
      181 cgggatgcgc tcgatcgagc cgtcaccgcc gcactcggcg accttcttcg gaaacgtgta
      241 cggcttcgca cccttcggcc gaagggccaa aacgacgctc acgatctccg ggatgacgtc
      301 tcccgccttc cggaggacga ccgtgtcgcc gatgcgcgca tcgagccgct tgatctggtc
      361 ctcgttgtgg agtgtcgccc gcgagacggt cgaacccgcg atgagaacgg gtcggaggtg
      421 cgcgaccggc gtgacgacgc cggttcgtcc gacctggagg acgatgtcct cgagaacggt
      481 cgtcgcctcc tccgagggga atttgtacgc gatgccgaag cgcggcgcct tcgccgtgta
      541 gccgagggcg ttctggaggc gaatgtcgtc gaccttgatg acggtgccgt ccatgccgta
      601 gtgcatcgag agcttcttcg gcgcccacct ccggtagtcg gcgatcgcat cgtcgagcgt
      661 cgcacagtgc ttcgcatgtg ggttgacggt gaacccgagc gacctaagga acgcgagttc
      721 ctcgtactgc gttgtcggca cgctcttccc gtcaagcgtt ctctcgtcga cgtagtcgac
      781 gtcgtacgcg aagaacgaga gtttccgtcc cttcgtgacc gtaggatcga gctggcggac
      841 cgacccggcc gccgcgttac gaggattggc gaagagcggt tcacccgacc tctctcgctc
      901 acggttgatc cgttcgaact cgcgccgcga gagccagacc tcgccgaccg cggtcagcgt
      961 gacgggcttc gcgagctcgg tcggcacatc ggcgatcatc cgcgcgctgt gcgtcacgtc
     1021 ctcgcccacg acgccgtcgc cgcgggtcgc ggcacggacg agcgcgccct tctcgtactc
     1081 gagcaccatc ttcaatccgt cgatcttatg ctcgctcacg tacgaaacgc gctccgacat
     1141 accctgcttc tcgagtgcgc gcatgaggcg ctcctcccac gcgcggagct cggcgtcgga
     1201 gaagacgtta tcgaacgacc actgtctgac gcgatgacgg accttcgcga acgcctcgct
     1261 cggttttccg ccgacgacct cggtcgcttc atagagatgc gcgaggtcgg ggtgcgcttc
     1321 ggtaatgccg cggagctcgc gcacgaggga atcgtacgcc tcgtcgctta tttcgggcgc
     1381 gtccttctcg tggtagagct tctggtggtg cgcgatgagg cgcgcgagct ccttcgcgcg
     1441 ccgctctgtc ggctttggtg ccatacgcgc taatagtaat acagaacggg ccctttagca
     1501 catgaaaacg gcggccgaag gccgccgttt gggtgccatc ttcgcctagt agacctgcgt
     1561 gtactcgcgc tcgacgacgc gcgggctcgt gcttatttgg ttacacggaa cgttgtaccc
     1621 gcgcgcctga atgaccgtac agacggagcc cgcgggacac gggtcgccgc ctcggaaatc
     1681 gcggttccct atcgtcacgg ggacgtccga accgcccgag gagaatttat acacgctcac
     1741 gatggagcag agcttgtccg cggcatcggg gtcgctcccc cacgtgaact cgaagcgctg
     1801 ttcgccgccg ctcatcgatt tgccatcggt ctcgttcgcg tccgagtagt tcgggaggtt
     1861 cgagccgtcg tccgatgacg cgttcacgcc catgcagtcg atgttcggaa cgaccgcctg
     1921 ttcgctccca tcatcgggaa cggcgaacgg gctcacgccg gccgccggaa aatcgtgata
     1981 gagcgcgcac tcgaggccgg cgttcgccgc ttggaaggcg atctcggagg cgagcgcgat
     2041 gcccgagagt tggtactgct tcagggtgat gttgatgagg gacgtgctca cgccgaggag
     2101 gactcccatg aggaggagcg tgatgaggag cgtgatgcct cgttcgtcgg ttggtacggt
     2161 tcgattaact ttcatagcgc gcatagtgat cgtatatcca cgtctcgagc gtgatggtcc
     2221 gcggcgtgcc gccgccgaac gtcgtcgacg tccacgtgac gctgaccgtg acgtcgatcg
     2281 cgtccggcgc ggcggcggtc acgtacacgc ggcgaacgta cccggagtcg gtcccgcccg
     2341 cctcgtagtt gtacgcgccc gcagcagtga ccttgagctc gcagttattg tcgagcgcgc
     2401 acgattcgaa cgcgtggctc gtgatatcga aggcgcaccc tgtcccgtcg gtacagtcgg
     2461 gcggtagcgc gccgatccag ttccacgtgt cgccaccgcc tgggttatcg tacgcggcga
     2521 gcgcggcgtc gtcgcgaagt tcccggatgg cctcgaccgc ctcctgtgcg aggaagaccg
     2581 cggtcacctg ctcgttcgcg tagtacgcgg cgcggatacc cttctgcgcg acggtgatgg
     2641 ggccgatgat gacgaggagg aggaccgtga tggcgaccaa cgtttcgacg agggtgaagc
     2701 cgtgtatgac gctcttgtgt tgtgtcgcgc ggtatatcat atgatatcga gtcgccgctg
     2761 tacgatatgg gtctgaatat agaaatcggt atcggtgtcg aggccgttgt tgacgtgtcc
     2821 cttcacgacg atgtcggcgg tgggctgctc ctcatcgttc ccaggactgt acgtcgtcga
     2881 attgtcgacc gttaccgaga actcgtcgac cgatacgtcg tccgcggtga tgggaaccca
     2941 gttgccgctc gactcctttt gctcgatcgc gtgcgtcgcc tcgtcgagac ggtaccccat
     3001 gcgcacgtcg tcctcctcgc ggatgaacga cacgaagtcg ccggcgtcgc agtcgttcgt
     3061 gaccgtcgag tccggaagcg cctcctcggg gttcccgctc gactccttgc agtagtagcg
     3121 ataccccgtg cgaatctcgc gcgtcatgga atcgagcgcg aaggagaggt tcgtcgtcgc
     3181 cgagtagacc gcctgtgcct tcgaattgag gtcgatgagg acgaggagcg tcccgacgct
     3241 gatcgtcatg acgatggaga acacggtgag agacaccatg agctcaacga gcgtgaagcc
     3301 cttctctctc tgctgtttcg tgattcgggt gctcatggcg ttattcctta tagacggaga
     3361 tctgaccgag attcgtcacc ttgaccgcgt ataccgaccc gggcgtcgcg gaggtgagct
     3421 tcaccgtccc gctgtcgatg gacgcgtcct cgacctgcgt gaacccgtcg acgtggaaga
     3481 gtcccttgaa cttcggccgc ctgaaggaga tgtcgagccg cgagagtccg cacgtctccg
     3541 taccgttctg atcgatgcag aggtctccga cgaccatcga gcgatcgagg aagaacgtcc
     3601 ggatgtcctc ggtgtcctcg ccgtagacgg ggaactcctt gtacgacgga tcgccctcgt
     3661 cggtgacgag gtagcggaag gactggtacg tcttaccgac gggcacgctc acgccgaacg
     3721 ggtagtcgaa gctcgtctcg tttcgcgcga ccgagaccga acggacctgc gcctcgcgga
     3781 gattgagcgc catctcgtac gcggcgcttt tgaggagcac gttgctgtcg aagctgttgt
     3841 acttcacgag aacgatggag gtcacgatgc cgatgatcgc gacggacacg agaagctcga
     3901 cgagcgtgaa ccccctaggg gaagaagacg aggtacgtga tgtcgaagat gtcagcatgg
     3961 aagaactgaa cgatgagaaa gccgagaacg agaaacggcg cgaagggcac ttcgctctta
     4021 attgtaagcg gtgcgccgag aaattgtaag cgcgcttttc cccgccgcag gagccgttcg
     4081 agaccgagaa gggcgagcgt aatgacggca ccgacccaga acgagaagac gagcatcgaa
     4141 aagacgcccc cgaggcccac catcatgccg agcggaaccg cgagcttcgc gtcgccgaag
     4201 cctatccacc gtccgcgtga gacataccag agcccataga agaaaaagcc ggcgctcgcc
     4261 cccgcgatga ggcgcgagag gacgacggcg acgtcgtgtc gggtgaacgc gtcgaagaat
     4321 gtgaagacga gggcgagcgc gaggaccgcc gcagtgagct cgttgggaat gatggtgtga
     4381 cggatatcgt aaaaaacgat gacgacgagg atggagacga ggacgagata gaagacgagg
     4441 agaaccggcg cgttcgcgaa gaggtgccac acccacgtaa acgagagtcc cgtaagcacc
     4501 tcgacgacga ggtagcgagg cgggatgtac gcgccgcact cgcggcaaat accgcgctgg
     4561 gcgaggtacg aaacgacggg aagaagttcc gaccacgaga gcgtctttcc gcacgagagg
     4621 cagtgcgaac ggccgcctaa tgacttcccc gtgtgaaagc ggtagatgac gacgttgagg
     4681 aaactcccca cgaccgcgcc gaggacgaag aacatgacgg tgaggtacgt ggccataggt
     4741 caggggcacg cgaccgcgcc cgaggcgagc gacccggtga cctgtcgcga ggcgttcgtc
     4801 gagtcgacgc accaggccgc gggcgatgcg gaggtcgagg agcgaagcgg cacccaaatg
     4861 gcccacgcgg cagcggtagc gtggcacgcc tcggagttcc agtcaccgac gtccgtgttc
     4921 gcgtaggcag ctgcgctcgg cgtgatgttc gccgccttcg ccgccgcctg aaccatcttc
     4981 cgaatgcgcg cgtcagcaca cgcatttagg tacgaattgt tgttattgac gtagtagagc
     5041 gcgatctgcg aacgcgcgtt tacgagattt tgctcgacac ccgcgttccc gcccgcgttc
     5101 ttctgctgcg atgtcgcgcc gatcaccacc gcggcgagta tcgcgatgat ggcgatgacg
     5161 acgacgagct caaggagcgt gaagccgcgt attgcggcgg aagagcgagg gtgtggcgaa
     5221 tgcatacgtg ttcatagtac cagacgcgct cgatggctcg cggcgcacct tcgccatcgt
     5281 gtgcacaacg caaaaagcgc ccggaggcgc tttttgcgtt aacaaacggt ggtttatccg
     5341 caggtccagt cggacgtatc ggtaagtcct ccgttcgcga ccgcagtggt cgtcgaatta
     5401 cctgtgctat cgacacagta ccaaagcgac gtgttcgact taagctgagc tgccgctccc
     5461 catgccgttc ccgcaccgcc gtcggcgcag acaacgctac cgctaccggc gttcgtcgcg
     5521 ccagtcatca tctgagagat gttgttcgtg cccgacgaac agacggttga gtacgaattg
     5581 ctgttgttcg tgtagaagag ctccgcctgt gcacgggcgt tgttgatgct cgacttgatc
     5641 gcggcgtcag cgcccttgtc gcgagccgag ttgagggacg cgagcacgac ggatgcgagg
     5701 atgccgatga tcgcgatcac cacgaggagc tcgatgagcg tgaagcctcg tttataaaga
     5761 ttggtcatca taggttatta aataagtaat atattactcc acgtagtgtg gttacctgta
     5821 attatacggg ctcgggcggc cccgaagccg taccctgtgg acaaagaacg gtttttagcc
     5881 cttatatgcc gcccgtgatg ttgtagatcg gaataaggac cgacgcgaga agcgttccca
     5941 cgccgaggcc gaggacgacg atcatgagag gctcgatgag gctgatgagc gtgtcgatcg
     6001 tgttgttgac ctcccggcgg tagaagaccg agagcgtgtt caatatatcg ccgagcttac
     6061 ccgtttcctc gccgaccttg ctcatctgcg cgaggacgcc cggtatctcc gggtactcgg
     6121 cgatagcatc cgcgaaggat ttacccgcct tcacgtcgac gagcgtgttg tcgatgatct
     6181 cgcggtatac gcggttatcg acgacctcgg cggtcacctc gagggcctgt accatcgaga
     6241 taccgctccc gagcatcgtc gagaggttat cgcagatgcg cgtgaggaag agcttctcgt
     6301 agagcgaccc gacgaacggg accgcgagct tgaactcgtc gatcatgtac tttccggcct
     6361 cggtcttctg gaactgccag aagactacgc cgaccaaggt gaggaacacg aggatgaggc
     6421 cgaagtagtc ggtcagtatc gacgagatac ccatgacgat gcgcgtgtag atgggtatct
     6481 cctgccccga gtcgatgagt atctggctga tcgacggaat gaccaaggtg agcatgagcg
     6541 tcatgacgcc cacgaagatg gagatgacga acgccgggta gatgagcgcg ttacgcgcct
     6601 tcgagacgac gaggtacatg cggtcgaggt agtccgcgag gtacatgaac gtcttgtcga
     6661 gcgttcccga ttcctcgccg gacttcacca tggagacgta gaactgcgag aatacgtcgg
     6721 gatgctgcgc gagggcgcgc gatatggaac taccgccctg gatggcatcg ctcacctcgg
     6781 tgaggatccg ctggagcttc tggttctcga cctcggagcc gaggaggcga aacacgcgaa
     6841 gcgccgagac ctgcgcctcg aagagagtcg cgatctgtcg tgagaggatg acgatgtcct
     6901 tattggagac gcggtcgaac cacgtgacct cgaaatcgag aatggaggac ttcccgccct
     6961 ccaccgggtc gacggtgatg atggtgtacc cgcgtccttc gacggtgtcg atcgccgctt
     7021 cgacgttctg ggcgtccacg gttccctctc ggggcgtccc gagcttatcg actgctttgt
     7081 atgaaaataa catggcgcta cattaatcgc tcgaggccct tcgggttcgt cgagtacgcg
     7141 aacgcgttct cgacggttat ctcacccttc tgcacgagac ttacgatcga acggttcatg
     7201 tcgatcatgc cctgctccat gccggtctcg atgaccgtct ggatctcgtg ggttcgccgc
     7261 tcgcggatga ggttcgcgac cgcggcgttc gagatgagga gctcgtacgc ggggacgaga
     7321 gagcccgaga tgcgcggaat gaggcgctgg gagaagattc cggtcaatga ccccgcgagc
     7381 tggacgcgta tctgatcctg ctgggtcgag gggaaactgt cgatgatgcg gtcgatggtc
     7441 tgcgcggcat tgttcgtgtg aagggtggag aagacgaggt gccccgtctc cgcggcggtg
     7501 accgcggtcg agatggtgtc ctgaccgcgc atctcgccga ccatgatgac gtcgacgtcc
     7561 tgccggaacg cggagtggag tccgacgtgg aagtccgcgg tgtcgacccg cacctcgcgc
     7621 tgatcgatca aggacttctt cggttcgaag atgtactcga tggggtcctc gatcgtgacg
     7681 atatgctcgg cgcggttctg gttgatgtgt tcgatcatcg ccgcgagggt cgtcgacttt
     7741 ccctgtccga cgggcccgac gacgaggaaa aagccctgcg ggcgctgcgt gaacgactcg
     7801 aggaccggcg ggagattgag ctcctcgaac gtcttgatga cgttcgagat gtaccggagc
     7861 gcgatggaca tcgagccgcg ctggaaaaaa ccgttcccgc ggaaacgggt tccctcgcgc
     7921 gtcgagaacg agaagtcgat ctcgcgcgat tcgagaaacc gctgttcctg ctccttcgtg
     7981 aggagaacgc tcacgaaacc ggcgacgtcg acgttcgtga ggaccggttt cttcgcgagg
     8041 ggtaccaagg agccgtcgac gcgaatgatg gggtgcgacc cgaccgagag gtggatatcg
     8101 gacccctttt cggacgtgac gatgtcaata aggtcgtcga gttctcgctt gtagtcaacg
     8161 gacaccatgt tcttatgtgt tacttggcaa cgagcttaag gacctgctcc accacctcgc
     8221 tcggaatgaa ctcggccttc acgatgtacc cgacggcgcc gtttccgagc gccgcttcct
     8281 tgtcggcgtc gtcgctctgg ttcgagagca tgatgcacgt cggattccca ccgaggttct
     8341 tcgactttat ctccttgagg agttcgatac cgttcatgcc gggcatcacc atgtccatga
     8401 ggatgacgtc gtacgtgccg ctctcgagct tcgcgagcgc gttctcggcc gtttgggcgg
     8461 cgtcgatcgt atgcccgagc tcgcggaact tcgtcgcgta catatcacga aggaaagcgt
     8521 cgtcgtcgac taagagtata ttcatatgcc cccattatac gcttcgaggg gtctcttttc
     8581 aggggttatc gacaggagct tcgggcgcgg gggcgtgcat ggcctccttc tccttctccg
     8641 cctcgtccga gacgaggagc tcaccgccta gggtgctcac ctcctcgaag ggaatgacgt
     8701 ggttgagcgc cttcacgatg gcgtcctcct tcatgctgat gaagccgttc gcccgggccg
     8761 ctttcagtat ctcgctctca tcgccgccat cgaggatgag cttctcgatg ctctcgctca
     8821 ccggtatcgc ctccatgacc gcgacgcggc ccttcgttcc cgacgagcac tccgcggagg
     8881 gctccgcgaa gaggacatcc tcggccttcg ggatgatctt tcgaaactcc gcggggagat
     8941 cgctgaactc cttctcgatc atgaccttga gactccccgt gaccggcgtc ttcttccccg
     9001 tccccgggca gatgcgccgg acgaggcgct gtgcgagcgc gagctcgagg gtcggcgcga
     9061 tgaggtacgg gtcgacgccc atgtcgatga gacgcggaat gacgccgacc gcgttgttcg
     9121 tgtggatggt cgagaggacg aggtggccgg tgagcgccgc ctggatggcg agctgggccg
     9181 tctccttatc gcgtatctca ccgaccatga tgacgtcggg gtcctggcgg agggcggtac
     9241 ggaggccgtt cgcgaacgtg tagccgatct ccgggcgtat ctgcgactga ctcacgccgt
     9301 cgatgttgta ctcgaccggg tcctcgagcg agatgacgtt cttcccgatg cggtcgacct
     9361 cggagagcat cgcgtagagc gtcgtcgact taccggagcc ggtcggaccc gagatgagga
     9421 tgatgccgta gggctgctgt atcgcgcggc gaatgagctc gatgttgtag tcggagacgc
     9481 cggtatcctc gagcgtgcgg acgcccgcgt cggtatcgag gatacgcatg accaccttct
     9541 ccccgtggtt cgtcgggagt atggaaacgc ggaagtcgat gggccgtccg tcgatcgacg
     9601 ccgagaagcg gccatcctgc ggctttcgct tctcgtcgag acgcaaggac gcgaggatct
     9661 tgatacgcgc gacgaccgcg gggtggacct tctgcgggag ttcgagcgag tggacgagga
     9721 caccgtcgac gcggaaacgg atgatggtct tctggtcgcg cggctcgatg tggatgtcgg
     9781 aggcgccgcc gtcgaccgcg tagcggagga tggtcgcgac tatcttcgtg accggggcgt
     9841 cctctttgat gtgctcgacc ttccccgcga cctcggtcgc cttatcctcc tccttcttga
     9901 gttcgctctc gagaacgccg agggcctcgt cgacctcgcc ggtgaggttc tcgtacgact
     9961 tcatgccgtt ctcgaaatcc cgctcgagga tgaacacgag cttgtacggg atctggtcct
    10021 tcatcgtgat gaaattgagc gcatcacggt acgagagatt ctcggggtcg gtgataccga
    10081 cgatgaggac gtcgtcctcg aacgcgatgg ggagaatgcc gtaatggcgg gcggattcct
    10141 cggagaggta cttgaggacg gcagagtcga tgcgctgggt ctccgtgatg gtgcgaatgg
    10201 gaagttcgta gtactcgccg atcgtctttc ggatgatatc gggatcgacg ccctcgtcga
    10261 tgagcgcctt ctcgtacgaa ccgccctctg cggcgcgcgc agagacgcgc tcctcgagcg
    10321 cggcgtttat cacgttcttt tctgcgagta acgcgagaag attcatacgg ctctctgccg
    10381 gcggtttatt ggaccggcgc gaacgggttc gagcggccgg taccgacgtc ggttatcggc
    10441 acgcgatagc tctgaagcga ggtgaaacgc gcatcgtcga agaccgagtc gtcgatcgta
    10501 tacgtgttta tcttttgaat atcggcgagt atcttctcgg tctgaaggac gacgtcatgc
    10561 ttctccgagg agatggccgt gaggtcggtg ctcccgccca tgaagaacgt cttgtagagc
    10621 acgccgacga cgaccgtggc gatgagggcg aagagcaggt tgcgtaggag gtgtgacata
    10681 cgatgtatta cttattcggg agcgcgtacg tgcgcacggt catcttgaac tgctggaacg
    10741 gcccctcggc gacggtaagc gagagctggg tcacctcgta gatggtgagg ctcttctcga
    10801 ggtccttgag gaagctcttg aactggtcgt acgtgccgac gaccgcgaac gagatgtcga
    10861 ccgtcgtgag ctcatcgcgc gaggtcgccg ctgaagacgc gccgcccccc gagcggtcga
    10921 cgaaggaaag ctgtttatcg tcgacctccg tcacctggaa aaggaggttg ttcttttgtg
    10981 cgagcgcctc gaggtcgacg aggtcctgtg tgtcgtctat ctcctcggga acgagctggt
    11041 cgagacgctc catctcgtac ggcgagcggt ccgacttctt cgcgagcttg ttctcgagcg
    11101 cgccggtgaa gtcggcgtac gtcttcgtcg cttggtggta ctcgtcgatc tggttcatga
    11161 ggtccttcgc cttcaggtac tgcggctgcg tgaagaacac gaagaggagg accgcgatga
    11221 gtatggaaag gatgggagtg gtggtgcgca tcatgattgt gtttggctat ccggagtctc
    11281 cggcgcgtcc acgggcgtcg tcgatgcgtc cgccggagcg gtcgcaggtt cgctcgacgt
    11341 atcagcctcg acggctggtg ccacgtcctc gggagcggag ctcggggcgg tttcttgcgg
    11401 tgccggttcg gacgccggga cgtcgtcccc gtagcttatg aggtcctttt tgaagacccc
    11461 gaccaccttg aaggagatac ccgcttgcgg cgcggtcgcg gtctctccct gcgccttcgc
    11521 ggtcttcgac gtcgtgatgt cttggagcac gaagtccgag aagaggctgt cttcgagtat
    11581 ctgcatcttc tggagcgcta tggaactgaa ctccttcgtg tcgccggtca gggtaagagt
    11641 cgcctcgaag cccgggtcgt tcacgaactc gagcgacctg tattggacgc tcttcttcgt
    11701 cgagttctct atctcctcga agatgcgcga tgtcgcgacg tggttatcga gaaggctcct
    11761 cgcgacgcgg aggcggtcgt tgtagagacg gatctcgtct atcttcttct gatttccgcc
    11821 gtcgcttacg gcaccgaggc tctgcttcgc gtcctcgagc cgcttctcga gcacgccctt
    11881 atagaggaaa atgccgaccg cacagaagag ggacagcaag aaaatgagcg tcgcggcgag
    11941 accgaaggcg tggatagtcc gcctcttttt catttggacc gggatggcgc tcgcggtctc
    12001 attgggaatg agcgaagaac gtgggatata tgccattccc tatagtatag cgctcgcgct
    12061 tcgcggaatg acgctcgctc tgtggacaga acgctacttc ctgtagacgc ggagcgcgac
    12121 accgaccgcg acggcgaacg atggaccggc gagtcgaagc gtgtcttcga gaaacgcggg
    12181 gtacgcgacc ttcgagaacg ggtccgagag gatgaccggg cgtgagaaca tgtcctcgag
    12241 gtacggacgg agacccgtga ggagagaacc gctccccgag aggacgacct tgtcgatctt
    12301 cgcgtggctc gtctcctcgt atcggctcat gaccgtgtga acctcgcgga agctccgctc
    12361 gacgcttccc gtgatggtct tctggagcgc ggcgtttccg tctcccccgc ccatgccgac
    12421 ggtgcgcttc agttcctcgg cgtctttgaa ctcgatgccc tcggtcttcg cgatgagatc
    12481 ggtgagctcg acgcccgaaa gggggacgct gtgcgccttc tcgacgacgc cgttacggac
    12541 gacgtagagc cgcgtcgcgg acgcgccaca gtcgaggatc gcgaccgtcg tgtccttcgg
    12601 cgagaggacc gagcgtatgg agctgaatat ctctatctcg ctcgcgaccg gcgtgaggcc
    12661 gcttccgtgc atgatggact ggtagcgctc gagcgtatcg ttgtagatcg cggagatgag
    12721 gacatcggtg cgtccctctt cctcgtgggg ggtgagcggg aaccagtcga ggctcacctt
    12781 cgtgagcgat atcggaatgt acttcttcgc ctcgatcggg atcatcgacg cgagccgttc
    12841 cgggtccgcg gtcggcaccg acgcgatggt gatgaagctc gcgttgtatg cgaacgcgaa
    12901 cgcggccttc gtcgcggtcg cgcccgattc gcggagaatg tcgaccaggg cctcgatcgt
    12961 cttcaccgga gtgaggtgcg tgccgcggcc gaggtctatc ccctcgtacg gaccgagctg
    13021 gagctctccg tacgtctcga gggtcggcac gccgcgttcg tccttaagct ggatcaactt
    13081 gatcgcggac gacccgatgt cgatgccgat gacgccgccc ttcgccgaag tcgccgcggt
    13141 gcgctgcggt ttgaggaacg atttgaggga cgaaagatcg aacgccgcca tatacgtgca
    13201 ctattctacc atcagatagt agtatgtacc tatggtgcat ttcctcacga gcgtcatcga
    13261 cgcgctcttc cctcccgagg gcgacgcgct cgtcgtccgc gagctcacag ggcgagaggt
    13321 atcgctcctc ttcgaaccgc gcgtcgtcga tggagtcgtc gcgcttacgc gattctcgga
    13381 cgcgcgcgtt cgcgcgctca tccatgaggc gaagttccat gggaacgccg ccgcgacgcg
    13441 ccacctcggc tccctccttg cccgctacct cgagcacgcg tcgtacgata tcgtcgtgcc
    13501 ggtgccgctc tcgaggcggc gcttcatgga gcgcgggtac aaccaggtcg cgcggatcat
    13561 agcggggagc gaagtgtccg gcgaccgcgt cgtctcttcc gcgctttcgc gtatgcgaca
    13621 cacgcgcccg cagaccgacc tcgagaagaa ggctcgcgcg gcgaacgtta gtggcgcgtt
    13681 cgcggtcgcg catgccgacg cgctccgcga caagcgcgtc atcctcgtcg acgacgtgac
    13741 gacgacgggt gcgacgctcg ccgcggcgag agaagcgctc ctcgccgctt ctcctcgctc
    13801 catcacctgc gtcgctttag cccattaggg taaaatgtgc tatcctaccc cggtcaaaat
    13861 tgaatattgg agacgtggct gagtgggtac atagtccggg ggactatgta ccggaatgca
    13921 tggcattccg tggacgactt gtcgaacacg aagccacgtc ggaatgacaa tggagacgtg
    13981 gctgagtggt cgaaagcgcc tcactgctaa cgaggtaggg cctaaaaacc ctcgacggtt
    14041 cgaatccgtc cgtctccgca ctcaatgcaa aaccggccct tgtggtcggt ttttgcaatg
    14101 agagacagga ttcgaagagg ttgccagcta ttttaaagaa tgaaaaatag cgacaacctg
    14161 tactgaagct gtaagcttcg aatccgtccg tctccgcaaa actagaaaat tcgggattct
    14221 tcccggattt tttagtttct gcgaaaagga ttcgaagacc ttgcgtgcta ctttatgagt
    14281 ggagcaaagt aaagtagccg caaggtgtac tgaacatgta atgttcgaat ccgtcattct
    14341 tgtgaaaaaa ataaccttct cttagaggac gggtcttgct agcatatact gatgaaatct
    14401 catcccttcg ttctcccatc gaagaaagcc gccggtcgca aaaacacgat aaagagcttc
    14461 aaggcgaagg cagacgcgcg gcgcacgacg accgaggtca tcgcggactt cctcacccgg
    14521 tcgttcggta cggtctcctt cttggtgctc aatgccatct ttttcatcgt ctggatcgca
    14581 tggaacatgg gtctcacgcc gttccccgtc atggacccgt tcccgtttgg cctcttgacc
    14641 atggtagttt ccctcg
//
LOCUS       DASTSP010000029         9782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_722122, whole genome shotgun sequence.
ACCESSION   DASTSP010000029 DASTSP010000000
VERSION     DASTSP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722122"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFS75_01190"
     CDS             complement(<1..1051)
                     /locus_tag="VFS75_01190"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEU4677314.1"
                     /translation="MELPHSLKRLYKMAAHHWLTASFILGFIVDNITLNRVDSLFDDV
                     LLFFYVLLAMVSIVLLYAGIAERYSERTNAFLKSKAPIVMQYAFGSILSGMLIFYGRS
                     GSFFDSWPFLVLIVGVILGNETITNRANRLVYNLIIFYIGLFSFVVLIVPVLIGKMGA
                     VIFVLSGLLSLILMGMLVRTLAHIVPNFIALEKKKLIFFVGTTFVLFNALYFTNIIPP
                     IPLSLKDIGIYHSVIRFENGHYELAYEKPAWWEFLRRSDSTFHYGAGDNVFCYASVFA
                     PARLAVDVYHRWQYYDESAGEWKDYGRFAYPIQGGRYDGYRGYTQISHVAPGDWRCIV
                     ETERGQVIGKREFTVE"
     gene            complement(1122..1943)
                     /locus_tag="VFS75_01195"
     CDS             complement(1122..1943)
                     /locus_tag="VFS75_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEU4677315.1"
                     /translation="MTLIRVSRNEAFALVRDGFVILTGYDEKPAERARSCFAEVLEVF
                     GAESLRRFQFEHERRPDGVREPDDGFLDWTRETELKKDRKFAFHYRPDLRKKMDAVRW
                     KSGEEAKYCLSRLLNLSAAVYNHHSEIAMAVLTAVDALELMSVCFADELKKALVAPAP
                     TSRSVVRFLYYPESSGNEKAKVHYDRSLLTLHSGDEGGSLFAVSNGHERVISPRPGEL
                     LVFWGSKASRLATKHGAKLKALAHGARAREGEVRQAVVSFWHNNDVLWDAEPVTM"
     gene            complement(2074..2508)
                     /locus_tag="VFS75_01200"
     CDS             complement(2074..2508)
                     /locus_tag="VFS75_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677316.1"
                     /translation="MKKGILCFMGTLALMIVAVLVWQPSKKSEPAPTVVRSSGCSEAG
                     ECSATLGWKDHSELYVTYQSGDKPIAVLNIQSGNLVLGEHAGRTEKLMAIAKKLALPQ
                     SSLAPAEEYMPGRHSRNIYTRITVPVAFGPKEFDAAMRALIK"
     gene            complement(2611..3876)
                     /gene="glyA"
                     /locus_tag="VFS75_01205"
     CDS             complement(2611..3876)
                     /gene="glyA"
                     /locus_tag="VFS75_01205"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU4677317.1"
                     /translation="MEYDHIERQDREVFSAIMGEEAREAKGLELIPSENYVSKAVREA
                     NGSVFTNKYSEGYPGKRYYGGQEFTDIVETIAIERAKRLFNCKFANVQPLSGAPANVA
                     MYFALLEPGDTVLGMHLEHGGHLTHGHPTTSINKVFNFVRYKMKDVDTGEIDYDELRN
                     VALEHKPKIILAGFSAYSRELDYKKFVEIAKEVGAYTVADMAHIAGLIAGGAVRNPFD
                     DGFDVVTTTTHKTLRGPRGGMILVRDDEELAKKINKSVFPGLQGGPHMNNIAAKAVAF
                     GEALTPSFKAYALQILRNAKAMEAVFRDANIRMLGGGTSNHLIVADVYGSLGVTGKEA
                     EVALDEIGITLNKNMIADEGRTAMDPSGIRFGTPAITTRNMREAEATEVAEIMVERLQ
                     HPKDEEVRTRLHTRIEALAAKFPIPETFM"
     gene            complement(3934..4275)
                     /locus_tag="VFS75_01210"
     CDS             complement(3934..4275)
                     /locus_tag="VFS75_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HEU4677318.1"
                     /translation="MELSDYQKRSRVTAIYPNAGANFVYPTLGLAGETGEVVEKVKKL
                     MRNEGIERADAVSPEWRVELAKEMGDVLWYLAQLATEFDLDLDDIAEGNLEKLLSRKE
                     RGVLHSEGDNR"
     gene            4333..5220
                     /gene="thyA"
                     /locus_tag="VFS75_01215"
     CDS             4333..5220
                     /gene="thyA"
                     /locus_tag="VFS75_01215"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU4677319.1"
                     /translation="MIKPYGERTPDTQYKNLLKEILAKGVRTPSRQGTDALTLVGATP
                     MHFRLENGFPMITERGVKGFWRQAIGEICGFVNGARTLEALESFGCKFWTPWGTKEKT
                     EKRGLKEGDLGPGSYGGAFHDFPTAEGVPYDQFKNIVEQIREFPELRTHFISPWVPQY
                     TIRGTGKQQKVVVCPCHGWIHIRIIDGKLYLHMFQRSGDVPVGVPSNMIQYAALTMML
                     AQVTGYEAYEYVHTISDAHIYVDQIPMVEELIARETKPFPTVTLTNPTKDLFAFRATD
                     FELADYEAGEPMKGIPVAV"
     gene            5224..5685
                     /locus_tag="VFS75_01220"
     CDS             5224..5685
                     /locus_tag="VFS75_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677320.1"
                     /translation="MEPSPKTGLNHLSARTEEQKALMAKIEEDGVCPFCAEHFRKYHP
                     KPILRETEYWFVTENMSPYEGTTHHFLFVYKPAHINRPDQMLPGAADDLMALVAWLEK
                     EFAIEGGSFFMRFGDTRTNGGSVSHLHAQLITGKPYQEGAEAIRVKLGWKS"
     gene            5757..6305
                     /locus_tag="VFS75_01225"
     CDS             5757..6305
                     /locus_tag="VFS75_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677321.1"
                     /translation="MEELKLPEPVKLTGERLICLQGCRSYKQYYEMRRRFEKDICTFC
                     ELPKENEIIWEDEHMRVWHVPLKYMREDELKYHFVIAPKRHVRRMEELTDDEAVSLHH
                     AHQIIGAKYHMPGGGYVTRFGAIAYNAGTVDHLHVNIYVPSGDNEFRVPLMKSPEQRE
                     ENRIRAAEFAARYDAGEVPETE"
     gene            complement(6357..7217)
                     /locus_tag="VFS75_01230"
     CDS             complement(6357..7217)
                     /locus_tag="VFS75_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEU4677322.1"
                     /translation="MQTRTIAIAYIPVLHRGYLAYLEKLEEDGVRELYLVGDELLASH
                     EELDYLNRKDRIRALPEATMLALLRSAFPSLSIELLSIANVMALHEGTPRIRAPREDV
                     NEFVMETYFDGHPISYDEVFLRWNKSNVEDATDADVERVSLDTFERDVFAKARGEATK
                     SADWWRQVGAALIKDGKVLYVAHNEHLPEEEYPNAVGDARSLYKKGDHVNYVTSAHAE
                     ASVIGLAAKEGVSTDGATLITTDFPCPFCARVIVKSGIARIFYEKGYPVLGGHTFFKE
                     MGIEVKKIET"
     gene            complement(7207..7740)
                     /locus_tag="VFS75_01235"
     CDS             complement(7207..7740)
                     /locus_tag="VFS75_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU4677323.1"
                     /translation="MSINTGIFGDTTSFEHRYIPDHERLRELVVYWKKLGLRIVLTSG
                     TFDLFHIGHAQYLEKAKALGDLLIVGVDSDEKVRARKGPHRPVVPEHERVKILTHCRH
                     VDVITLKAADHPKNHLIKIVSPDILVVSKSTEHPEGAVDEKAAFCGEIVVLEPQAETS
                     TSAKIRLIQIEKGENAD"
     gene            7813..8316
                     /locus_tag="VFS75_01240"
     CDS             7813..8316
                     /locus_tag="VFS75_01240"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU4677324.1"
                     /translation="MENPRVSIVAALGKNRELGRGNDLIWRIKEDLGRVKRLTMGHPI
                     VMGRNTYESIGHPLPGRTNIVISSAAGDIPGCIVAPSLEAALAAARATGTDEVFIFGG
                     ARVYKDALPLTDRLYLTLVDGGAADADVFFPDYADFTTIIEEERHDEHEPPFRWVTLE
                     RPASSSV"
     gene            complement(8282..9013)
                     /locus_tag="VFS75_01245"
     CDS             complement(8282..9013)
                     /locus_tag="VFS75_01245"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEU4677325.1"
                     /translation="MKFIPTRTSVLEPPRTDLPTFLDESIRNLEDEDVVIITSKVVAI
                     SEGRYVRMDAANKDDLVRAEADYIYERQGRKPLTVVHHALISAAGIDESNGFGHYVLL
                     PKDPFESARRIRTHLMARFSLRELGVVITDSHSLPCRYGAMSVSIGSWGFEPVESHVG
                     RRDLFGRVMRYSSLNIADSIAAASALVSGECDERTPVIIARDVPNLVFSDACPREKLF
                     VPFDEDIYEPLFREFKPTRKRDVRA"
     gene            complement(9197..>9782)
                     /locus_tag="VFS75_01250"
     CDS             complement(9197..>9782)
                     /locus_tag="VFS75_01250"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4677326.1"
                     /translation="RVLESTHFTCDTPVEEVFGDQTFLERPTIAGLIINGGAMLMVQP
                     TKVRSEKEERQWIPPQGGVKFGADGDETLLTAALREFGEELGLTTEHLVPRKAPELIG
                     HCRNPIPAERGESFEFKRIFFIVMHARSRDWVKLNGENRKYLWVDSGPYLLSLIGSMF
                     ERRETKFQAICAATVEAHKRGMLPWDCRLEPAPA"
BASE COUNT         1985 a         2939 c         2971 g         1887 t
ORIGIN      
        1 cttcgacggt gaactcgcgc tttccgatga cctggccgcg ctccgtctcg acgatacagc
       61 gccaatcgcc cggcgcgacg tgcgatatct gcgtgtaccc gcggtacccg tcgtagcggc
      121 ctccctggat cgggtacgcg aagcgcccgt agtccttcca ctcgcccgca ctctcgtcgt
      181 agtactgcca ccgatggtag acgtcgaccg cgaggcgcgc gggcgcgaag accgacgcgt
      241 aacagaagac gttgtcgccc gcgccgtagt ggaacgtcga atccgaacgc cgaagaaact
      301 cccaccacgc tggtttctcg tacgcgagct catagtgccc gttctcgaat cggataacgc
      361 tgtggtagat accgatgtcc ttgagggaga gcgggatggg cggaatgatg ttcgtgaagt
      421 agagcgcatt gaagagtacg aacgtcgtcc cgacgaagaa tatgagcttc ttcttctcga
      481 gcgcgatgaa gttcggcacg atatgcgcga gcgttcggac gagcatcccc atgaggatga
      541 gcgagagtag cccgcttagc acgaagatga ccgcacccat cttgccgatg agaaccggta
      601 cgatgaggac gacgaacgag aagagcccga tgtagaagat gatgaggttg tagacgagcc
      661 ggttcgcccg gttcgtgatg gtctcgttgc cgagaatgac gccgacgatg agcacgagga
      721 acggccagct gtcgaagaac gaaccgctcc gtccatagaa gatgagcata ccggagagga
      781 tgctaccgaa cgcgtactgc atgacgatcg gggccttcga tttgaggaac gcgttcgtcc
      841 gctcgctgta gcgctccgcg atacccgcgt agaggaggac gatcgagacc atcgcgagaa
      901 gaacgtagaa gaagaggagg acgtcatcga acaggctatc gacgcgattg agcgtgatgt
      961 tgtcgacgat gaagccgagg atgaaggacg cggtgagcca gtggtgcgcc gccatcttat
     1021 aaagtcgttt gagcgagtgc gggagctcca tagcgacatt gtagtatcct cgcggataaa
     1081 agaaaatgcg cccgaaggcg catgaggagg gcgtacgcgg tctacatcgt gaccggttcc
     1141 gcatcccaga gaacatcgtt gttgtgccag aaggacacga ccgcctggcg cacctcaccc
     1201 tctcgtgctc gagctccgtg tgcgagcgct ttgagcttcg cgccgtgctt cgtcgcgagg
     1261 cgcgatgcct tcgagcccca gaagacgaga agctctccgg gtcgaggcga tatgacccgc
     1321 tcatgcccgt tcgagaccgc gaagagcgaa cctccctcgt cccccgaatg gagcgtgaga
     1381 agcgagcggt cgtagtgcac cttcgccttc tcgttccccg acgattccgg gtagtagagg
     1441 aagcgaacga cggagcgcga cgtgggagcg ggagcgacaa gcgccttctt aagctcgtcg
     1501 gcgaagcaga ccgacatgag ctcgagcgca tcgacggcgg taaggacggc catcgcgatc
     1561 tcgctgtggt ggttgtagac cgccgcggag agattgagaa ggcgcgagag acaatacttc
     1621 gcctcttcgc cgctcttcca cctgaccgcg tccatcttct tccggaggtc cgggcgatag
     1681 tggaacgcga acttcctatc cttcttgagt tccgtttcgc gggtccagtc gagaaagccg
     1741 tcgtcgggct ccctgacccc gtccggacgc cgctcgtgct cgaactggaa gcgccggagc
     1801 gattcagcgc cgaacacctc gagaacttca gcgaagcaac ttcgtgcgcg ttcggccggt
     1861 ttttcgtcgt accccgtaag gatgacgaag ccgtcccgaa cgagcgcgaa ggcctcgttc
     1921 cgcgacaccc gtatgagggt cattgcgttc cctttccaaa aaaacgtgtc tagcaggtac
     1981 tctagcagag tctgcggcgc tcgacaaaaa gctcatgagg ggacgcaaaa aagaaaccgc
     2041 ctagcttgtg ggctagacgg cacaactttt cgttcacttg atgagcgcac gcatcgccgc
     2101 atcgaactct tttggtccga aggcgacagg cacggtgata cgggtgtaga tgttcctact
     2161 gtgcctgccg ggcatatact cttctgcggg agcgagcgag ctctgcggca gcgcgagctt
     2221 cttcgcgata gccataagct tttcagtccg tcctgcgtgc tcgccgagaa ctagattccc
     2281 ggactggatg ttgagcaccg cgatgggctt gtcgccgctt tggtacgtca cgtagagctc
     2341 cgagtggtcc ttccacccga gcgtcgcgct acattctcca gcctcactgc agccagagga
     2401 gcgcacgacg gtgggcgcag gttcggactt cttgctcggc tgccatacga gaacggcgac
     2461 gatcatgagc gcaagcgttc ccatgaagca cagaatgccc tttttcatcc ctacctccaa
     2521 tgaacactat ataaataata ttatacaata ccaaaacaat acctgtcaat attcaggtta
     2581 aggctctaat gagcgctttt tcgactacaa ctacatgaag gtctcgggga tggggaactt
     2641 cgcggcgagc gcctcgatac gtgtgtggag gcgcgtccgc acctcctcgt ccttcgggtg
     2701 ctggaggcgc tccaccatga tctcggcgac ctcggtcgcc tcggcctcgc gcatgttccg
     2761 cgtcgtgatg gcgggcgtgc cgaagcgaat gccggaaggg tccatcgcgg tccgcccctc
     2821 gtccgcgatc atgttcttgt tgagcgtgat gccgatctcg tcgagcgcga cctcggcttc
     2881 cttccccgtc acgccgaggg agccgtagac gtccgcgacg atgaggtggt tcgaggtgcc
     2941 gccgccgagc atgcggatgt tcgcgtcgcg gaagaccgct tccatcgcct tcgcgtttcg
     3001 gagtatttgg agcgcgtacg ccttgaagga cggcgtgagc gcctcgccga acgcgaccgc
     3061 cttcgcggcg atgttgttca tgtgggggcc gccctgaagg cccgggaaga ccgacttgtt
     3121 gatcttcttc gcgagctctt cgtcgtcgcg gacgagtatc atgccgccac gcggaccgcg
     3181 taaggtcttg tgggtcgtcg tcgtcacgac gtcgaagccg tcgtcgaagg ggtttcggac
     3241 cgcgccaccc gcgatgagac cggcgatgtg cgccatgtcg gcgaccgtgt acgcgccgac
     3301 ctccttcgct atctcgacga acttcttgta gtcgagctcg cggctgtacg ccgagaagcc
     3361 ggcgaggatg atcttcggct tatgctcgag ggcgacattc cgaagttcgt cgtagtcgat
     3421 ctcacccgtg tcgacgtctt tcatcttgta gcggacaaaa ttgaatacct tattgatgga
     3481 ggtcgtcgga tggccatggg tgaggtgccc gccatgctcg aggtgcatac cgaggacggt
     3541 gtcgcccggc tcgaggagcg cgaaatacat cgcgacgttc gcgggcgcac cggagagcgg
     3601 ctgcacgttc gcgaacttac agttgaagag cctcttcgcg cgctctatcg cgatcgtctc
     3661 gacgatgtcg gtgaactcct gcccgccgta gtagcgcttt ccgggatatc cctcggagta
     3721 cttgttcgtg aagaccgagc cgttcgcctc gcggaccgcc ttcgaaacgt agttctccga
     3781 cgggatgagc tcaagaccct tcgcctcacg cgcttcctcc cccatgatcg ccgagaacac
     3841 ctccctatcc tgcctctcta tgtgatcgta ttccatgtga aaaatctatc gtaagtaagg
     3901 tcgttcggtt gtgcggtctc agatcatggt gcgctatcgg ttatcgcctt cgctatggag
     3961 gacgccgcgt tccttccggg agagaagttt ctcgaggttc ccttccgcga tgtcatcgag
     4021 atcgaggtcg aactccgtcg caagctgtgc gaggtaccag aggacgtcgc ccatctcctt
     4081 cgcgagttcc acgcgccact cgggcgagac ggcgtccgcg cgttcgatac cttcgttccg
     4141 catgagcttc ttcaccttct cgacgacctc gcccgtctcg cctgcgaggc cgagggtcgg
     4201 atacacgaag ttcgctccag cattggggta gatcgcggtc acgcgcgagc gcttctgata
     4261 gtcgctaagc tccatatcga gacaatagta gcaccatttg aagggagggg gcaaagacgc
     4321 tacaattcgg cgatgataaa gccctacggc gagcggacgc ccgacacgca gtacaagaat
     4381 cttttaaagg agatcctcgc aaagggcgtt cgcacgccct cgcgccaggg cacggacgcc
     4441 cttaccctcg tgggagcgac gcccatgcac ttccgcctcg agaacggctt tccgatgatc
     4501 accgagcggg gcgtgaaagg cttctggcgt caggcgatcg gcgagatatg cgggttcgta
     4561 aacggtgcgc gaacgctcga agcgctcgag tccttcggct gtaagttctg gacgccctgg
     4621 ggcacgaagg agaagacgga gaagcgcggg ctcaaagagg gcgacctcgg gccgggttcc
     4681 tatggcggcg ctttccatga cttcccgacc gcggagggcg tgccgtacga ccagttcaag
     4741 aacatcgtcg agcagatacg ggagttcccc gagctccgca cgcacttcat ctcgccgtgg
     4801 gtgccgcagt acacgattcg cgggaccggc aaacagcaga aggtcgtcgt gtgtccgtgc
     4861 cacggatgga tacacatccg catcatcgac gggaaactct acctccacat gttccaacgc
     4921 tcgggcgacg tgcccgtcgg cgtcccgtcg aacatgatcc agtacgcggc gctcacgatg
     4981 atgctcgcgc aggtgacggg gtacgaggcg tacgagtacg tccataccat ctccgacgcg
     5041 cacatctacg tcgaccagat accgatggtc gaggaattga tcgcgcgcga gacgaagccc
     5101 ttcccgacgg tcacgctcac gaacccgacc aaggacctct tcgccttccg cgcgaccgac
     5161 ttcgagctcg ccgactacga ggccggcgaa ccgatgaagg gtatccccgt cgccgtctga
     5221 tatatggaac cgagcccgaa aacaggtttg aaccacttga gcgcgcgaac cgaggagcag
     5281 aaggccttga tggcgaagat cgaggaggac ggggtctgtc ccttttgtgc ggagcacttt
     5341 agaaagtatc atccgaaacc gatacttcga gagaccgagt actggttcgt gaccgagaac
     5401 atgagcccgt acgaaggtac gacgcaccac ttcctcttcg tgtataaacc ggcacatatc
     5461 aatcgacccg accagatgct tccaggcgcc gcggacgacc ttatggccct ggtcgcgtgg
     5521 ctcgagaagg aattcgcgat cgagggtggc agtttcttca tgcgcttcgg cgacacgcga
     5581 acgaacggcg ggagcgtctc acacctccat gcgcaactca tcacggggaa accctaccag
     5641 gaaggcgccg aagccatccg cgtgaagctc ggctggaaat cttgacattt aactagtgcg
     5701 tggcatgctg tcacgcagac taaattccgt cccaccaccg taacgaggag gcacacgtgg
     5761 aagaactgaa gctgcccgaa ccggtcaaat tgaccggtga acgcctgatc tgcctgcagg
     5821 gttgcagaag ctacaaacag tactacgaga tgcgacggcg tttcgagaag gacatctgca
     5881 ccttttgcga acttcccaag gagaacgaga tcatctggga ggacgagcac atgcgagtct
     5941 ggcatgtccc cctcaaatac atgcgggagg acgagctcaa gtaccacttc gtcatcgcgc
     6001 cgaagcggca cgtgcggcgg atggaggagc tcaccgacga cgaagcggtc tcgctccacc
     6061 atgcgcatca gatcatcggc gcgaagtacc acatgccggg cggcggatac gtgacgcgtt
     6121 tcggtgcgat agcgtataac gccggcaccg tcgaccacct ccacgtgaac atctacgtac
     6181 cgagcggcga caatgagttc cgcgttccgc tcatgaagag cccggagcag cgggaggaga
     6241 acaggatacg cgccgcggag ttcgcggcgc ggtacgatgc gggcgaggta ccggaaacgg
     6301 agtgaaggtg ccccaaagga ccatacaaag cgcgcgacct cggtcgcgcg ctttttctat
     6361 gtttcgatct tcttcacctc gatacccatt tccttgaaga aggtatgacc gccgaggacc
     6421 ggatagcctt tctcataaaa aatgcgcgcg atgccgctct tgacgatgac gcgcgcgcag
     6481 aacggacacg gaaaatcggt cgtgatgagc gtcgcgccgt cggtcgatac gccttccttc
     6541 gccgcgaggc cgatgaccga tgcctcggcg tgcgcggagg tcacgtagtt cacgtggtcg
     6601 ccctttttgt agagggagcg agcgtcaccc accgcgttcg gatactcctc ctccggtaaa
     6661 tgctcgttgt gcgcgacgta gaggaccttg ccgtccttga tgagcgcggc gcctacctgc
     6721 cgccaccagt cggcgctctt cgtcgcttcg ccccgcgctt tcgcgaagac gtctcgttcg
     6781 aacgtatcga gggaaacgcg ttcaacgtcc gcatcggtcg catcttcgac gttgctcttg
     6841 ttccagcgaa gaaagacttc gtcgtatgag ataggatggc cgtcgaagta cgtctccatg
     6901 acgaactcgt tcacgtcctc gcgcggtgcg cgaatacgcg gggtcccctc atggagcgcc
     6961 atgacgttcg cgatcgagag gagttcgata gagagcgagg ggaacgcgga tcgaaggagc
     7021 gcgagcatcg tcgcctcggg aagcgctcga atgcggtcct tgcggttcag gtagtcgagt
     7081 tcctcatgcg aggcgagaag ctcgtcgccg acgagataga gttcccggac gccgtcctcc
     7141 tcgagcttct caaggtacgc gaggtagccc cgatggagaa cggggatgta cgcgatcgcg
     7201 atggtcctag tctgcatttt ctcctttctc tatttgaatg aggcgtatct tcgccgacgt
     7261 cgaggtctcc gcctgcggtt cgaggacgac gatctcgccg cagaacgcgg ccttctcgtc
     7321 gaccgctcct tccggatgct cggtcgattt cgagacgacg agaatgtccg gcgagactat
     7381 cttgatgagg tggttcttcg gatggtccgc cgccttcagg gtgatgacgt cgacgtgccg
     7441 gcagtgcgtg aggatcttca cccgttcatg ctcgggaacg acgggccgat gcggaccctt
     7501 ccgtgcgcgg accttctcgt cgctgtcgac gccgacgatg agaaggtcac cgagcgcctt
     7561 cgccttctcg aggtactgcg cgtgtccgat gtggaagaga tcgaacgtcc ccgaggtgag
     7621 gacgatgcgg aggccgagct tcttccagta cacgacgagc tcgcggaggc gctcgtggtc
     7681 ggggatgtaa cgatgctcga agctcgtcgt gtcgccgaaa atacccgtgt tgatcgacat
     7741 ggaagcagta tagcggacgc cccattccgg ctcaaggagc gacgcatcgc ggcatgtgtc
     7801 ataatgtacg ccatggaaaa cccgcgtgtg agcatcgtcg ccgccctagg caaaaaccgc
     7861 gaactcggcc gcgggaacga cctcatctgg cgcatcaaag aggacctcgg ccgcgtgaag
     7921 cggctcacca tgggccaccc catcgtcatg ggacgaaaca cgtacgagtc gataggacac
     7981 cccctcccgg gccggacgaa catcgtcatt tcctcggccg caggcgacat ccccggatgc
     8041 atcgtcgcgc cctcactcga ggccgcgctt gcggcagcac gcgcgacggg cactgatgag
     8101 gtcttcatct tcggcggcgc gcgcgtatac aaagacgctc tcccgctcac cgaccggctc
     8161 tacctcacgc tcgtcgacgg aggagccgcg gacgccgacg tcttcttccc cgactacgcc
     8221 gacttcacga ctatcatcga agaggaacgt cacgacgagc acgaaccccc gtttcggtgg
     8281 gtcacgctcg aacgtcccgc ttcctcgtcg gtttgaactc tcgaaaaagt ggctcgtaga
     8341 tatcctcatc aaaaggcacg aagagcttct ctcgcggaca cgcatccgag aacacaaggt
     8401 tcggaacatc gcgagcgatg atgaccggcg ttcgctcgtc gcattctccg gagaccaggg
     8461 cggacgcggc cgcgatggaa tcggcgatat tgagcgacga gtaccgcatg acgcgaccga
     8521 agagatcgcg cctcccaacg tggctctcga ccggttcgaa tccccatgag cctatagaga
     8581 cgctcatggc tccgtagcga cacggaagcg agtggctatc cgtaatgacg acacccagtt
     8641 cgcggagcga gaaccgcgcc atgagatgcg tacgaatccg ccgagcgctc tcgaatgggt
     8701 ccttagggag gagcacgtag tggccgaacc cattactctc gtcgatgcct gccgccgaga
     8761 tgagcgcatg gtggacgacg gtgagcggct tacggccctg ccgctcgtag atgtagtccg
     8821 cctcggcgcg aacgaggtcg tctttgttcg ccgcgtccat acgcacgtac cgcccttcgc
     8881 ttatggcgac gaccttcgac gtgatgatga cgacgtcctc gtcctcaagg tttctgatcg
     8941 attcgtcgag gaaggtgggt aggtcggtac ggggtggctc gaggacgctc gtcctcgttg
     9001 gaatgaattt catggagttg tctctaatgt ataagcaccg atagaggcgc cacacgaaga
     9061 acgaacgcct ctcgctagga gagtgagagc tcggagaaca tggaccaaat acgtttgtgt
     9121 ccagtgagga gcatagtaca tagagtataa caaaaaagga gcggccgaag ccaccccttc
     9181 aaaagaacga atgccgttat gccggcgccg gctcgagcct gcagtcccac ggcaacatgc
     9241 cccgcttgtg cgcctccacg gtcgccgcgc agatcgcctg gaacttcgtc tcccgacgct
     9301 cgaacatcga accgatgagc gaaaggaggt acggacccga atccacccag aggtacttcc
     9361 ggttctcacc gttcaacttc acccagtcgc gcgaccgcgc gtgcatgacg atgaagaaga
     9421 tgcgcttgaa ctcgaagctc tcgccacgct cggcggggat cggattgcgg cagtgcccga
     9481 tgagctcggg cgccttccgc gggacgagat gctccgtggt gagcccgagc tcttcaccga
     9541 actcgcggag cgcggcagtg aggagcgttt cgtctccgtc ggcgccgaac ttcacaccgc
     9601 cttggggcgg gatccactgg cgttcttcct tctccgaccg caccttcgtc ggctgcacca
     9661 tgagcatggc accgccattg atgatgagtc cagcgatcgt ggggcgctcg aggaatgtct
     9721 gatctccgaa cacctcctcg accggtgtgt cacacgtgaa gtgggtgctc tcgagcaccc
     9781 tt
//
LOCUS       DASTSP010000030         4709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1839476, whole genome shotgun sequence.
ACCESSION   DASTSP010000030 DASTSP010000000
VERSION     DASTSP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839476"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1382)
                     /locus_tag="VFS75_01255"
     CDS             complement(45..1382)
                     /locus_tag="VFS75_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677327.1"
                     /translation="MIHLFEESSEFLEKFGLPSKYALLGIGFALAAIAYGYFDLESSR
                     VYEFMVNTAPIWAPIITFFLFFEKWLEYVRLKFNVKRPRVTLEIKLPQEVLKSPEAME
                     LVLNQLHQGATPDNHIETYVDGKHPPKFGLEIVSRGGDVRFYINVQKPKFKNITETQL
                     YAQYPGIEVHELDIDYTAEIPWDPERFKYFSIHFGHKKPTGFPIKTYVDYELQEMPKE
                     EEKIDPITSMLEMLGSIGPGEYVWVQMLIEANRKVTFKEGALVEKPDWTKEGRAQVEG
                     VIKDAAKRVGVQEDEIGRNVNQFLTDEEKETIRAMERGLSKNAFNTAIRCMYIAESDA
                     YNPGERIGAIITGWRSYDYIGRNAIGVRWRTDFDWNWWQDPSGHEREHLKHNELEEYK
                     KRTYTNYSHADLPTPFSVEELATMYHFPGTVASTPSLARLPSKRSEAPSNLPT"
     gene            complement(1453..2478)
                     /locus_tag="VFS75_01260"
     CDS             complement(1453..2478)
                     /locus_tag="VFS75_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU4677328.1"
                     /translation="MITFKPKVVTKKLLSVLPERARDVLKKRYGLGTTPETFTLESIG
                     QTYGITRERVRQIENYGIQAIQKSDAYAECQNVFDELENALHTLGGVISEDELLSELA
                     EDPSLRNHIYFLLVVGDPFKRYKEDNSFTHRWCVEKRIADSVEKALKQIFSTLGEDDL
                     ISEDEIIARLKASLGEIEERYRDEKILRRWLSISKLIDRNPLGEWGHATSPNVRAKGI
                     RDYAYLAVKRHGSPMHFKEVAEAIQSLFEKKAHVATTHNELIKDKRFVLVGRGLYALA
                     EWGYSAGVVKDVLRDILRTHGPLTREEIIDKVRKERYVKDNTIIVNLQDTSLFKKLPD
                     GNFILVE"
     gene            complement(2570..>4709)
                     /gene="leuS"
                     /locus_tag="VFS75_01265"
     CDS             complement(2570..>4709)
                     /gene="leuS"
                     /locus_tag="VFS75_01265"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU4677329.1"
                     /translation="KEWTDANVTYMTKQLRSMGNAFSWDHAARSTDPEYFSWTQWMFT
                     RFFEEGIAYRGTGIVNWCPGCNTVIANEQVTSEGCCERSGDQVEKREMPQWMLRITKY
                     ADRLIDDLDALPWPEHVKEAQRQWIGRSVGAKLSFELRFERHPEDNERRGPHGEKAAI
                     PVFTTRPDTLFGATYLVLAPEHPWVTLATDDAHDVLDNKDEVRTYVDEARQRDEKERT
                     DATKEKTGVELKGVKAVNPATGEEIPMYVADYVLAGYGTGAIMAVPAHDERDYAFATK
                     FGLLIRQVIDTDGELPVTTAGKLINSGEFDGIASEEAKRAIVERFGGEPTSTYRLRDW
                     SIGRQRYWGVPVPIVYDPEGNPHSIPKEHLPWTLPTDVDFKPTGEPPLAKSVELQERT
                     ERIFGPGWTPDVETMDTFVDSSWYFLRYVDPHDSDSFSSKEAQRAWMPVDLYFGGAEH
                     TTMHLLYSRFWQKALYDLGLVTDQEPYTRRVNRGLVLGPDGAKMSKSKGNVIDPDREV
                     ERLGADAVKMYLAFMGPYGDGANYPWDMGGIAGLRRFLERANGLRAHVRTTVGDDIAV
                     LLNRTIAKVTGDIDAYKFNTAISALMIFQNAAEKDGLTDDEYCTFLRLLAPFAPHLSE
                     ELWHEAGKTASVHEEAWPTADPALLEESTVTVAVQVNGKTRGTIEVPRDADESAVLNA
                     ALADPLVGPKLAGERKRVIYVANKVLNVVL"
BASE COUNT          815 a         1409 c         1443 g         1042 t
ORIGIN      
        1 cgtgcggcgc gtgtacgcga cgatgatcgg ttcgggtcgt cgcatcatgt cggcaggttc
       61 gacggcgctt cggagcgctt cgagggcagg cgtgccaagg acggcgtcga cgcgaccgtt
      121 cccgggaagt ggtacatcgt cgcgagctct tcgaccgaga acggcgtcgg gaggtcggca
      181 tggctgtagt tcgtgtacgt acgcttcttg tactcctcaa gctcattgtg cttgaggtgc
      241 tcgcgctcgt gcccggatgg gtcctgccac cagttccaat cgaaatcggt tcgccaacgc
      301 acgcctatcg cgtttcgccc tatgtagtcg taacttctcc agccggtgat gatggcgccg
      361 atgcgttctc ccgggttgta tgcgtcgctc tccgctatgt acatacaccg aatcgccgta
      421 ttgaacgcgt tcttcgagag gccgcgttcc atcgcacgga tggtctcctt ctcttcgtcg
      481 gtgaggaatt ggttcacgtt tcgacctatc tcgtcctcct ggacgccgac gcgcttcgcc
      541 gcgtccttga tgacgccttc gacctgcgct cttccctcct tcgtccaatc gggtttctcg
      601 acgagcgcac cctccttgaa ggtgaccttt cggttcgcct cgatgagcat ctggacccat
      661 acgtactcac ccggtccgat ggagccgagc atctcgagca tcgaggtgat ggggtctatc
      721 ttctcctctt ccttcggcat ctcctggagc tcgtagtcga cataggtctt tatgggaaag
      781 ccggtcggtt tcttgtgtcc gaagtggatg gaaaaatact tgaagcgctc ggggtcccac
      841 ggtatctcgg ccgtgtagtc gatgtcgagc tcatggacct cgataccggg atactgcgcg
      901 tagagctggg tctccgtgat gttcttgaac ttcggtttct ggacgttgat gtagaaacgc
      961 acgtcgccgc cacgggagac tatctcgagg ccgaacttcg gcggatgttt cccatcgacg
     1021 tacgtctcga tgtggttatc cggcgtcgcg ccctggtgga gctgattgag cacgagctcc
     1081 atggcttcgg gcgacttgag gacctcctgg ggaagcttga tctcgagggt gacgcgcggc
     1141 cgcttcacgt tgaacttcaa gcgtacgtac tcgagccact tctcgaagaa gaggaagaac
     1201 gtgatgatgg gggcccagat cggcgccgtg ttgaccatga actcgtacac gcggctggac
     1261 tcgaggtcga agtatccata cgcgatggca gcgagagcga acccgatgcc gaggagcgcg
     1321 tacttcgacg ggagaccgaa cttctcgagg aattcggacg attcttcgaa gagatggatc
     1381 atggatgtag gtccattgta caacaaaccg ccggaaagac aggggtcttt ccggcggttt
     1441 gtgagtagtc gcctactcga cgaggatgaa gttcccgtca gggagcttct tgaagagcga
     1501 cgtgtcctga aggttcacga ttatcgtatt gtccttcacg tagcgctcct tcctgacctt
     1561 gtcgatgatc tcctcgcgcg tgagggggcc atgggtgcga agaatgtcgc ggagtacgtc
     1621 tttcacgacg cctgccgagt atccccactc ggcgagcgcg tagagcccgc gtccgacgag
     1681 gacgaaacgc ttgtctttga tgagctcgtt atgtgtcgtc gcgacgtgcg ccttcttctc
     1741 gaaaagcgac tgtatcgcct cggcaacctc cttaaagtgc atcggcgagc catggcgctt
     1801 cacggcgagg tacgcgtagt cacgaatacc cttcgcgcgc acgttcgggc tcgtcgcgtg
     1861 tccccactcg ccgagcggat tgcggtcgat gagcttcgat atggaaagcc agcggcgaag
     1921 tatcttctcg tcgcggtacc gctcctcgat ctcgccgagc gaggccttga gacgcgcgat
     1981 gatctcatct tcggagatga ggtcgtcctc gccgagggtc gagaatatct gcttgagcgc
     2041 cttctcgacg gaatccgcga tacgcttctc gacgcaccag cggtgcgtga acgaattgtc
     2101 ctccttgtac cgcttgaacg ggtcgccgac gacgaggagg aagtaaatgt ggttacgaag
     2161 cgaagggtcc tccgcgagtt ccgagaggag ctcatcctcg ctgatgacac cgcccaaggt
     2221 atggagcgcg ttctcgagct cgtcgaacac gttctggcac tccgcgtacg cgtcggactt
     2281 ctggatcgcc tggatgccgt agttctcgat ctggcggacg cgctcacgcg tgatgccgta
     2341 cgtctggccg atggattcga gggtgaaggt ctccggcgtc gtgccgagtc cgtagcgctt
     2401 cttgaggaca tcgcgagcac gctccggaag gacggagaga agtttcttgg tgacgacttt
     2461 cggtttgaac gtgatcatga tgaatgtgtc gaaatggtga cctcctccga aatatgtggt
     2521 cttattttta tcacgggtat cgattcgcgt caagaagacg ccgttcgggc tacaggacga
     2581 cgttgagaac cttgttcgcg acgtagatga cacgtttcct ctcccccgcg agcttcggtc
     2641 cgaccagagg gtcggcgagc gccgcgttaa ggacggcgga ctcgtcagcg tcgcggggaa
     2701 cctcgatcgt tccgcgagtc tttccgttca cctgcacggc gacggtcacc gtgctctcct
     2761 caaggagagc cgggtcggcg gtcggccacg cctcctcatg aacggacgcc gtcttccctg
     2821 cctcgtgcca gagctcttcg gagaggtgcg gtgcgaacgg cgcgaggagc cgaaggaacg
     2881 tgcagtactc gtcgtcggtg agcccgtcct tctccgcggc gttttggaat atcatgagcg
     2941 cactgatagc cgtattgaac ttgtacgcgt cgatgtcgcc ggtgaccttc gcgatggttc
     3001 gattgaggag taccgcaatg tcgtcgccca cagttgtcct cacatgggcg cggagcccgt
     3061 tcgcgcgctc gagaaagcgg cgcagtcccg cgatgccgcc catgtcccac gggtagttcg
     3121 ctccgtctcc gtacggaccc atgaaggcaa ggtacatctt gaccgcgtcc gcaccgaggc
     3181 gttcgacctc cctatcgggg tcgatgacgt ttcccttcga cttcgacatc ttcgcaccgt
     3241 cagggccgag gacgaggcca cggttgaccc gcctcgtgta cggctcctgg tcggtgacga
     3301 gtccgaggtc gtagagcgcc ttctgccaaa aacgcgagta gaggaggtgc atcgtcgtat
     3361 gttcagcgcc gccgaagtag aggtcgacgg gcatccacgc tcgctgggcc tccttcgagg
     3421 agaaggaatc cgaatcgtgc gggtcgacgt agcgaaggaa gtaccacgat gagtcgacga
     3481 aggtgtccat cgtctcgacg tcgggcgtcc agcctggtcc gaagatgcgt tcggtacgct
     3541 cttgaagctc gacgctcttc gcgagaggcg gttcgccggt cggcttgaaa tcgacgtcgg
     3601 tcggcaaggt ccacgggagg tgttccttcg gtatggagtg cgggttcccc tccgggtcgt
     3661 acacgatggg cacgggcacg ccccagtagc gctgtcggcc gatagaccag tcgcggaggc
     3721 gatacgtgct cgtcggctcg ccaccgaagc gctcgacgat agcgcgcttc gcctcttcgg
     3781 atgcaatccc gtcgaactca ccggagttga tgagcttccc cgcggttgtc actggaagtt
     3841 cgccgtcagt atcgatgacc tgccgtatca ggagaccgaa cttggtcgcg aaggcatagt
     3901 cgcgctcatc atgggcgggc accgccatga tggcgcccgt tccgtagccc gcgagaacgt
     3961 agtccgcgac gtacatcggt atctcttctc cggtcgcggg attcaccgcc ttcacgccct
     4021 tcaattcgac accggtcttc tccttggtcg cgtcggtgcg ttccttctca tctcgctgtc
     4081 gagcctcgtc gacgtacgtc cggacctcgt ccttgttgtc gaggacgtca tgcgcatcgt
     4141 cggtcgcgag cgtgacccac ggatgctcgg gtgcgagcac gaggtacgtc gcgccgaaga
     4201 gcgtatccgg gcgcgtcgtg aatacgggaa tcgcggcttt ctcaccatgc ggaccgcgac
     4261 gctcgttatc ctcgggatgg cgctcgaacc ggagctcgaa cgagagcttc gcgccgacgc
     4321 ttcgccctat ccactgccgc tgtgcctcct tcacgtgctc gggccacggc aaggcgtcaa
     4381 gatcgtcgat gagccgatct gcgtacttcg tgatgcggag catccactgc ggcatctcgc
     4441 gtttctctac ctggtctccg gagcgttcac agcacccttc gctcgtcacc tgctcgttcg
     4501 cgatcaccgt attgcacccg ggacaccagt tcacgatgcc cgtaccgcgg tacgcgatac
     4561 cttcctcgaa gaaacgcgtg aacatccact gcgtccacga aaagtactct gggtccgtgg
     4621 agcgcgccgc gtggtcccac gagaacgcgt tccccatgct ccggagctgt ttcgtcatat
     4681 aggtgacgtt cgcgtccgtc cactccttc
//
LOCUS       DASTSP010000031         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1840312, whole genome shotgun sequence.
ACCESSION   DASTSP010000031 DASTSP010000000
VERSION     DASTSP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840312"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..173
                     /locus_tag="VFS75_01270"
     tRNA            90..173
                     /locus_tag="VFS75_01270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Leu,seq:cag)
     gene            389..688
                     /locus_tag="VFS75_01275"
     CDS             389..688
                     /locus_tag="VFS75_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677330.1"
                     /translation="MEETALDLERFRRDYGEYVIEVIPEEQAKFGPRFSFDGWFVIPM
                     ANSDDTFLVDSGGWGGIAFQLRVDFKTNACYLEPETEGGEWTFVGVLLPKNARPA"
     gene            complement(702..1724)
                     /locus_tag="VFS75_01280"
     CDS             complement(702..1724)
                     /locus_tag="VFS75_01280"
                     /inference="COORDINATES: protein motif:HMM:NF001133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU4677331.1"
                     /translation="MKRSRVPNSVRKREVSEAKIPRRTLSFYRYVRIADPKAMRDLLY
                     GKFMELGCLGRIYVAGEGINAQMNVPCARFDAFDAYLNSLPEFANMPYKLAVEETDVV
                     SFYKLTVKVREKIVADGLDDASFDVTKTGEYLTAREMNAYVDDPDAVIVDMRNGYESE
                     VGHFENAITPDVDTFREELEVVPELLRVHKKKKIALYCTGGIRCEKASAWLRHNGFEN
                     VKHLRGGIIDYKRQVEEEKLPNRFLGKNFVFDERLGERISGEVIAHCHLCNTAKCDEH
                     THCRNDACHALFICCPSCNARLNGYCSYRCRLFDALPSGIKTRLTRNDHKKREVRYRK
                     RRLRSV"
     gene            1905..2996
                     /locus_tag="VFS75_01285"
     CDS             1905..2996
                     /locus_tag="VFS75_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677332.1"
                     /translation="MATAHVPPQVDELVQTDDLVKRDRFGRAVVTWSSPVVFVFLGYV
                     AGTFTSVLLHLPYFIGQSLGLTCGTYAAGIALSRSFVRNDATIAFITVNPLASLFGKN
                     PLVVYGPGTHFCYWWEQRSADNVVSLVEATEPFTAVVQTGSGTVTFQGSTRLRPNICE
                     LHQFISGAAAAASNITGLIIAHIVGFTGASDGGNKPKPLLEVLKNIGGLNDELRKEFK
                     EGRAGSKVSDFERRFGVIVGDTTVEKILPSEDVQKTMSAVTESEVMDTMVAKSFGCAS
                     YEELVKKVEDGTFNWATVNQVRTQKMAASGNLQGMDLKDHTFNLNVNGLDQLSPEAAA
                     AFASVLGRFETFKGTRGKGAGGNRPNERR"
     gene            3159..3584
                     /locus_tag="VFS75_01290"
     CDS             3159..3584
                     /locus_tag="VFS75_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677333.1"
                     /translation="MAIDAYHEAAAERAVKKEKAPPTMRNGEGRVYTTPLAEPAPTRY
                     SFTATHDKWTVIEMVQGRCIFMHVTDLGRHDRNGHDVAVEVGGINQGAWIPFTEYLAA
                     VHAHRTPFDAGFLRVRTVGDEPIDMTFEFRPWGTCDTRS"
     gene            complement(3642..3929)
                     /locus_tag="VFS75_01295"
     CDS             complement(3642..3929)
                     /locus_tag="VFS75_01295"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677334.1"
                     /translation="MIAYAADSADAVAEAHGFLTRLENAILFPLMTLMMAVAFLIFLW
                     GGYEYIANAADDGGRETGRKHMLYGIIGLVIMVSAYGILKIAASTFGVPVE"
     gene            complement(3936..4310)
                     /locus_tag="VFS75_01300"
     CDS             complement(3936..4310)
                     /locus_tag="VFS75_01300"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEU4677335.1"
                     /translation="MKIIAYLLPLVLIVPMTAFAQTNPGNLTPVTTFVRNIIDFINGT
                     LVPFIFAIAFLVFLWGVFKTFILGGSDEGKQEEGKKLMMYAIAGFVIMVSLWGIVNLL
                     ANGLGFSGADIQKIPDAPKTNN"
BASE COUNT          984 a         1240 c         1251 g          937 t
ORIGIN      
        1 gaatcgaaga ggtcgtcgac ggatttagta gaatccgtca tacgacctga acaggttctg
       61 taagaaccga tctccacccc gggcaccaag ggcgtgtggc gtaattggta gccgcgcacg
      121 gttcaggtcc gtgtgtccga aaggacatgg aagttcgagt cttctcacgc ccacaaaata
      181 aagagccatc gcggttcttt atttttgggc aaaagactcg aagctgttca gagatagttc
      241 gagcttgcga gaaatatcct gaacgggtac tgaacacgta atgttcgagt cttctcacgc
      301 ccacaaaata aagagccatc gcggttcttt atttttgggc acaaggttca gttggaaagt
      361 tcttcactac ttctatgaag gaggtaccat ggaggaaacg gcgctcgatc tcgagcggtt
      421 tcgcagagac tacggagaat acgtcatcga ggtgataccg gaagagcagg caaagttcgg
      481 tcctcgtttc tcgttcgacg gctggttcgt tatccccatg gcgaactcgg acgacacgtt
      541 cctcgtcgat tcgggtggat ggggcggcat cgcgttccaa ctacgcgtcg acttcaaaac
      601 gaacgcgtgc tacctcgaac ccgaaacgga aggaggagag tggaccttcg tcggcgtact
      661 cctaccgaag aatgccaggc ccgcgtaagc gggctttttc tttatacgct tcgaagacgg
      721 cgctttcggt agcgtacttc ccgcttcttg tggtcgttcc tcgtaaggcg agtttttatg
      781 cccgagggaa gcgcgtcgaa aagccgacag cggtacgagc agtacccgtt caacctcgcg
      841 ttgcacgagg ggcagcaaat gaaaagcgcg tggcaggcgt cattccggca atgcgtgtgt
      901 tcgtcgcact tcgccgtgtt acagaggtgg caatgcgcga tgacctcgcc gctgatccgc
      961 tcgcccaagc gctcatcgaa gacgaagttc ttcccaagaa agcgattcgg gagcttctcc
     1021 tcctctacct gccgcttgta gtcgatgatg ccgccccgaa ggtgcttcac gttctcaaag
     1081 ccgttatggc gaagccacgc gctcgccttc tcacagcgga taccgcccgt gcagtagaga
     1141 gcgatcttct tttttttatg tactcgaagg agctccggaa cgacctcgag ctcctcgcgg
     1201 aacgtgtcga cgtccggcgt gatggcgttc tcgaagtgcc ccacctcgct ctcgtagccg
     1261 tttcgcatat cgacgatgac cgcgtcggga tcgtcgacgt acgcgttcat ctcgcgcgca
     1321 gtgaggtact cgccggtctt cgtcacatcg aacgacgcgt cgtcaagccc gtccgcgacg
     1381 atcttctctc gcaccttcac ggtgagcttg tagaacgaga cgacatcggt ctcctcgacg
     1441 gcaagtttgt atggcatgtt cgcgaactcc ggaaggctat tgaggtacgc gtcgaacgca
     1501 tcgaaccgcg cacacggtac gttcatctgc gcgttaatgc cttcccccgc gacgtagatg
     1561 cgcccgagac agccgagttc catgaacttc ccgtagagca ggtcgcgcat agccttcggg
     1621 tccgcgatgc gcacgtaccg atagaacgac aacgtcctcc gcggtatctt cgcttcacta
     1681 acctctcgtt ttcgcaccga gttcggcacc ctgctccgtt tcatgacggc cagcatagcg
     1741 catttcgacc aagtccgaaa gatggaacgc cgaaaggagc gctctgtcgg gccctaaaaa
     1801 cggccctcgc tcattgactt ttgatgtatt tcatggtatc attgaaaaag aataggattc
     1861 ggtagtattt cacttcccat ccacgctcat tgaaaggata cacaatggca actgcccacg
     1921 tgccgccgca ggttgacgag ctcgtacaaa cggatgacct agtaaaacgc gaccgctttg
     1981 gacgagcggt cgtcacgtgg tcatctcctg tcgtcttcgt ctttctgggc tatgtcgcgg
     2041 ggacgttcac gtcggtcctg ttgcacctgc cctatttcat cggacagagt ctcggactca
     2101 cgtgtgggac ttatgctgcc ggcatcgcgt tgtcccgttc cttcgtccga aacgacgcta
     2161 cgatcgcgtt cattaccgtg aatccgcttg catcgctttt cggcaagaat cccctcgtcg
     2221 tatatggacc ggggacgcac ttctgctact ggtgggagca acggtctgcg gacaatgtcg
     2281 tgagcctcgt cgaggcgacc gaaccgttca cggctgttgt acagaccggg agcggaaccg
     2341 tcacctttca ggggtcgacg cgtctccggc cgaacatctg cgaactgcat cagttcatca
     2401 gcggtgcggc ggcggcggcc tcgaacatca cgggtctgat catcgcgcac atcgtcggtt
     2461 tcacgggagc gagcgacggc ggaaacaagc ccaagccgct acttgaagtc ctcaagaaca
     2521 tcggcggcct taacgacgag ctcaggaagg agttcaagga gggtcgagca gggagtaagg
     2581 tctcggactt cgagcgtcgc ttcggcgtca tcgtcggtga taccaccgtc gaaaagatcc
     2641 tcccatctga agatgttcaa aaaacgatga gcgccgtcac tgagtccgag gtgatggaca
     2701 cgatggtcgc caagtcgttc ggatgcgctt cttacgagga gctggtcaag aaggtcgaag
     2761 atggcacgtt caactgggcg acggtcaacc aagtccgcac tcagaagatg gccgcgtccg
     2821 gcaatcttca gggcatggac ctcaaggatc ataccttcaa cctgaatgtg aacggacttg
     2881 accaactgag cccggaggca gcggctgcct ttgccagtgt tctcggtcgc ttcgaaactt
     2941 tcaaaggcac ccgagggaaa ggagccggag gaaatcggcc caacgaaagg agatgacgga
     3001 tggcgaacgt acggtcgcag accattgcgg aagtgcaaca gcaaggccgc gctcaagcaa
     3061 cggcgacgca taacacgagc aaaggcacgt tcaagaaatg ggctacgcgc gtcgcaatca
     3121 tgttgggcat ttcctatctc gtttcggtag gtatggatat ggctatcgat gcctaccacg
     3181 aggcggccgc ggaacgggcg gtgaaaaagg aaaaagcgcc accgaccatg cgaaacggcg
     3241 aaggtcgtgt ctacacgaca ccgctcgcag agccggcgcc gacgcgctac tcgttcaccg
     3301 cgacgcatga caagtggacg gtcatcgaga tggtccaggg tcgttgtatc ttcatgcacg
     3361 tcacggacct cggaaggcac gaccgaaacg gccacgacgt agccgtcgag gtcggcggca
     3421 tcaaccaagg tgcctggatt cccttcaccg agtatctcgc agcggtccac gcgcacagga
     3481 ccccgttcga cgccgggttc ttgagggtcc gtaccgtcgg cgatgagccg atcgatatga
     3541 ctttcgagtt ccggccatgg ggtacgtgcg atacgcgttc ttgacgcaca aagcgcgatg
     3601 tgagctaaca catcgcgctt tttcttgtca cgatctatgc ctcactcgac cgggacaccg
     3661 aaggtgcttg ccgcgatctt gagaatgccg tatgcggaga ccatgatgac gaggccgatg
     3721 atgccataga gcatatgctt ccgacccgtc tccctgccgc catcgtccgc ggcgttcgcg
     3781 atgtactcgt accctcccca aaggaagatg aggaacgcga ctgccatcat gagggtcatg
     3841 agcgggaaga gaatcgcgtt ctcgagccgc gtcagaaaac cgtgcgcctc cgctacggcg
     3901 tccgccgaat ctgccgcgta cgcgatcata cgagcttaat tgttcgtctt cggagcatcc
     3961 ggtatcttct ggatatcagc accgctgaaa cctaagccgt tagcgaggag attcacgata
     4021 ccccagagcg agaccatgat aacgaaccca gcgatcgcgt acatcatgag cttcttgccc
     4081 tcctcctgct tcccctcgtc ggagcccccg aggatgaacg tcttgaaaac gccccagagg
     4141 aagacgagaa acgctatcgc gaagatgaac gggacgagcg ttccgttgat gaaatcgatg
     4201 atgtttcgga cgaacgtagt aactggagtt aggttgccag gattcgtctg cgcgaacgcc
     4261 gtcatcggca caatgagaac tagcgggagt aagtaagcga tgattttcat actgtgtaaa
     4321 cgctgtaaaa cgtgggatat tattaacgat attaagaacc ttgtacctat tctagccttt
     4381 atgggaagta ccggggggta gtactgtgga ta
//
LOCUS       DASTSP010000032         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1184148, whole genome shotgun sequence.
ACCESSION   DASTSP010000032 DASTSP010000000
VERSION     DASTSP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184148"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VFS75_01305"
     CDS             complement(<1..175)
                     /locus_tag="VFS75_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEU4677336.1"
                     /translation="MNLLHDIPCGSADEMNVIVEIPKFSKNKYEIDKETGIIALDRVM
                     HTAQDYPFDYGFVP"
     gene            complement(197..1405)
                     /gene="nusA"
                     /locus_tag="VFS75_01310"
     CDS             complement(197..1405)
                     /gene="nusA"
                     /locus_tag="VFS75_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU4677337.1"
                     /translation="MFDLKVIHSVLDQMEEERGIGREKMLDAIEQALATAYKKEFGKR
                     GQIIRATFDLNSGDTTFNQVKIVADETTVRMDDQVDHEAEGENQPEREETPGEDGDLR
                     VRFDPEKHILIENARLIKKDVQVGDELVFPLESKEDFGRIAAQTAKQVIIQKIREAEK
                     ESALKEFGQKEGDIVVGHVQRFERGNLYVDLGRVTGVMPYDEQIPGERYHPGERVRAL
                     LVAVDETPRGIFLKLSRSAPDFLSKLFEIETPELQNGAVEIKAIAREAGSRSKVAVYA
                     HDSHIDPVGSMVGQRGVRVSTVMSELGGEKIDIIEWSEKPEEFIEDALSPAQVLSVEV
                     LEEPGENEDERGHAQVTVAPDQQSLAIGRGGQNVRLAAKLTGWKIDIGSANAGGDADA
                     TNTDEGAKEA"
     gene            1703..2293
                     /locus_tag="VFS75_01315"
     CDS             1703..2293
                     /locus_tag="VFS75_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677338.1"
                     /translation="MLHLQLISRLPADTRNALLEPLIAQERKEDPESLKFGQAEEFGH
                     LMVPEDYVQSKFIDETRARYREQKGVVVLDNWNGAWFTLSSHELRPGERITVTAVRQY
                     AKAATLGEQLQYLDTQGAVYPSTHGLLLFFDQFRSEMTMNTWYVALDAESRLPMMQHQ
                     RCVLMARRELRRSVLKVQSVAHVGPDVRILCFRQGF"
     gene            2438..4153
                     /locus_tag="VFS75_01320"
     CDS             2438..4153
                     /locus_tag="VFS75_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU4677339.1"
                     /translation="MDSGLVSRKDVDAAEKLATEEKRPLAEALVSGGYISEDDLRRVE
                     SYVLGVPFVSLRDAKIPFEVLSLIPEPVARTHNIIAFKKAEDSLEVAMLDTADLPAID
                     FVKKKVGLKILPRLTDTESMKKALLQYQKTLKDEFGDLIVKEASALKVVAENPDEKTS
                     EEELKKMAEDLPVVRIVDTLLRHAIIQGASDIHIEPMENELLVRYRIDGILHDAMSLP
                     KHAASTISARIKVLSNLKLDEKRLPQDGRFKMDMDGQKVSFRVSVLPIFFGEKIVMRL
                     LREGRSGFTLEGLGFHGQTLERVHDATKQTTGIILVTGPTGSGKTTTLYTVLDILNTP
                     KVNISTIEDPIEYQMPRVNQTQVKPEIGFTFANGLRSLMRQDPDVIMVGEIRDQETAS
                     LAINAALTGHLVLATVHTNSSSATIARLIDMGVETFLLVSTLRIALGQRLVRRLTETK
                     EPYTLSANERANLGKHIDLDRVLQVLKKENIVKSSATWNDIPFYRPKEGVDSDEGYKG
                     RIGIHEVLQVSPTLKELILAEGTADQVEAQARKEGMLTMLEDGIYKAALGQTSIEEVL
                     RVISE"
     gene            4159..>4687
                     /locus_tag="VFS75_01325"
     CDS             4159..>4687
                     /locus_tag="VFS75_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU4677340.1"
                     /translation="MEKFTYTGTTKDGQPVTETVEAADKYAVYEIARTNGHTVTNVAP
                     ATRISVKSLVNMDRLNVLLSRVKADELVMMTRNLSSMLKAGLPLSRALSVIERQSKNP
                     RLKSTLASIRESIAKGEPFFEALKKFPETFSPLYVAMVRAGEESGGMVDTLRMISLQL
                     ERSSQLRKKIKGAMTY"
BASE COUNT          914 a         1454 c         1402 g          917 t
ORIGIN      
        1 gcggcacgaa gccgtagtcg aacgggtagt cctgcgccgt gtgcatgacg cggtcgagcg
       61 cgatgatgcc cgtctccttg tcgatctcgt acttgttctt cgagaacttc ggtatctcga
      121 cgatgacgtt catctcgtcg gcgcttccgc acggaatgtc atgtaagagg ttcatatggt
      181 gatgattacg ctggtattac gcttccttcg ccccctcgtc cgtattggtc gcgtcggcat
      241 caccgcccgc gttcgcggac ccgatatcga tcttccaacc ggtcaatttc gcggcgaggc
      301 gtacgttctg tccgccacgg ccgatcgcga gggactgctg atcgggcgcg acggtgacct
      361 gcgcgtgacc gcgctcatcc tcgttctcgc ccggttcctc gaggacttcg acggaaagga
      421 cctgtgcggg agagagcgcg tcctcgatga actcctccgg cttctccgac cactcgatga
      481 tgtctatctt ctcgccgccg agctcgctca tgacggtcga gacgcgcacg ccgcgctggc
      541 cgaccatcga accgacgggg tcgatgtggc tatcatgggc gtacacggcg accttcgagc
      601 gcgagcccgc ttcgcgggcg atcgccttga tctcgaccgc gccgttctgg agttcgggcg
      661 tctcgatctc gaagagcttc gagagaaaat caggcgcgga gcgcgagagc ttgagaaaaa
      721 tgccgcgcgg cgtctcgtcg acggcgacga ggagcgcgcg aacgcgctca cccggatggt
      781 atcgctcgcc ggggatctgc tcgtcgtacg gcatgacgcc ggtcacgcga ccgagatcga
      841 cgtagagatt cccgcgctcg aagcgctgga cgtggccgac gacgatgtcg ccctccttct
      901 ggccgaactc cttgagcgcc gactccttct cggcctcgcg tatcttctgg atgatgacct
      961 gcttggccgt ttgcgcggcg atgcggccga agtcctcctt cgattcgaga gggaagacga
     1021 gctcgtcgcc cacctgaacg tcctttttta tgagacgcgc gttctcgatg aggatgtgct
     1081 tctcggggtc gaagcggacg cggaggtcgc cgtcctcgcc tggcgtctcc tcgcgctccg
     1141 gctgattctc cccctcggcc tcgtggtcga cctggtcgtc catacggacg gtcgtctcgt
     1201 cggcgactat ctttacctgg ttgaacgtcg tgtcaccgga attgaggtcg aacgtcgcgc
     1261 gaatgatctg tccgcgcttc ccgaactcct tcttatacgc ggtcgccaaa gcctgctcga
     1321 tggcgtcgag catcttctcg cggccgatgc cgcgctcctc ctccatctga tcgagaaccg
     1381 agtgaatgac tttaagatcg aacataccta tatatatgtg gggtataccc cgtttatagc
     1441 tcctaaatcc cgttaaaggt aacaaaaatg ccggctggta gagccgacct catgtgtacc
     1501 ttcgagtata gcggaagcga acgggaagtc aaataatgcg gcggtttgca ggaaggtgtg
     1561 aaaggcgcta gcattcgaag ggttccatac gtatgttcca attccttgat cggaggattc
     1621 gatgcagacg gacgacaaga tcctccgaga gttattcgcg gaggctcaga agcggatctt
     1681 taaggggcaa gatcgcacct atttgcttca tttacagctc ataagccgcc tcccggccga
     1741 cacccggaac gcgctcctcg aaccgctcat cgcacaagaa cggaaagagg accccgagag
     1801 cctgaagttc ggtcaagcgg aggagttcgg tcacctcatg gtgcccgaag actacgtcca
     1861 gtcgaagttc atcgatgaga cgcgcgcgcg gtaccgtgag caaaagggtg tcgtggtcct
     1921 cgataactgg aacggagcgt ggttcacgct ttcgtcccac gagctccgtc ccggcgagcg
     1981 tatcacggtg accgcggtcc ggcagtacgc gaaagcggcg accttggggg agcagcttca
     2041 gtacctcgat acacaaggag cggtctaccc gagcacgcat ggtctcttac tgttcttcga
     2101 ccagtttcgg agcgaaatga ccatgaacac gtggtacgtc gcgctcgatg ccgagtcgcg
     2161 gcttcccatg atgcagcatc agcgctgcgt gctcatggcg cggcgcgaat tacgtcgctc
     2221 cgtcctcaag gtgcagtcgg ttgcccacgt cggaccggac gtccgcatac tctgcttcag
     2281 acaaggcttt tgagcccgca tcccgcgggc tctttttctt tttcactcac accggcgcgc
     2341 cgcaccgcgt ccctcggatg ctatactgat ggcgtaatcc catacgggga ggttcgttac
     2401 tgtgcgcata caggacacac aaatcaaacg atttgtcatg gattcggggc tcgtctcccg
     2461 gaaggacgtt gacgctgcgg agaagctcgc gaccgaggag aagcgaccgc tcgccgaggc
     2521 cctcgtctcg ggcggctaca tctccgagga cgacctccga cgcgtcgagt cgtacgtcct
     2581 cggcgtgccg ttcgtctcac tccgcgacgc gaagataccg ttcgaggttc tctcccttat
     2641 ccccgagccg gtcgcgcgga cccataacat catcgcgttc aagaaggccg aggattccct
     2701 cgaggtagcg atgcttgata cggcggacct tcccgcgatc gacttcgtga agaagaaggt
     2761 cggtctcaag atcctcccgc gtctcaccga caccgagtcg atgaagaagg cgctcctcca
     2821 gtaccagaag acgctcaagg acgagttcgg cgacctcatc gtgaaggagg cgtccgctct
     2881 caaggtcgtc gcggaaaacc ccgacgagaa gacgtccgag gaggaattga agaagatggc
     2941 ggaggacctt ccggtcgttc gcatcgtcga cacgctcctt cggcacgcga tcatccaggg
     3001 cgcgtcggac atccacatcg agccgatgga gaacgagctc ctcgtccggt accgcatcga
     3061 cggcatcctc cacgacgcga tgtcgctccc gaagcacgcc gcgtcgacca tctccgcgcg
     3121 catcaaggtc ctctcgaact tgaagctcga cgagaagcgc cttccgcagg acggccgctt
     3181 caagatggac atggacggtc agaaggtgtc gttccgcgtc tcggtcctcc ccatcttctt
     3241 cggtgagaag atcgtcatgc gcctccttcg agaggggcgt tcgggattca cgctcgaggg
     3301 cctcggcttc catgggcaga ctttggagcg cgttcacgac gcgacgaagc agacgaccgg
     3361 catcatcctc gtcaccggtc cgaccgggtc gggtaagacg acgacgctct ataccgtcct
     3421 cgatatcttg aacaccccga aagtcaacat ttcgaccatc gaggatccga tcgagtacca
     3481 gatgccgcgc gtcaatcaga cgcaggtgaa gcccgagatc gggttcacgt tcgcgaacgg
     3541 actccgctcg ctcatgcgcc aggatccgga cgtcattatg gtcggtgaga tccgtgacca
     3601 ggagacggca tcgctcgcca tcaacgcggc cctcaccggc cacctcgtcc tcgcgacggt
     3661 ccatacgaac tcgtcctcgg cgaccatcgc acgtctcatc gacatgggcg tcgagacgtt
     3721 cctcctcgtc tcgaccctcc gcatcgccct cggacagcgc ctcgttcgcc gccttacgga
     3781 gacgaaggag ccgtacacgc tttcggcgaa cgagcgcgcg aatctcggga agcacatcga
     3841 cctcgaccgc gtgctccagg tcttgaagaa ggagaacatc gtgaagtcgt cggcgacgtg
     3901 gaacgatatc ccgttctacc ggccgaagga gggggtggat tcggacgagg ggtataaggg
     3961 ccgcatcggc attcacgagg tcttgcaggt atcgccgacc ctcaaggagc tcatcctcgc
     4021 ggagggaacc gccgatcagg tcgaggcgca ggcgcgcaaa gagggcatgc tcacgatgct
     4081 cgaggacggc atctataagg cggcgctcgg tcagacgtcg atcgaggaag ttctccgcgt
     4141 catctcggaa tagttttcat ggaaaaattc acctacacgg gaacgacgaa ggacggtcag
     4201 ccggtcaccg agaccgtcga ggcggccgac aagtacgcgg tctacgagat cgcgcgcacg
     4261 aacgggcaca cggtaacgaa cgtcgccccc gcgacgcgca tttcggtgaa gagcctcgtc
     4321 aacatggatc gcctcaacgt gctcctctcg cgcgtgaagg cggacgagct cgtcatgatg
     4381 acgagaaacc tctcgtcgat gctgaaggcc ggtctcccgc tgtcccgcgc gctctcggtc
     4441 atcgaacgac agtcgaagaa tccgcggctt aagagcacgc tcgcttcgat tcgcgagtct
     4501 atcgcgaagg gagagccgtt cttcgaggcg ttgaagaaat ttcccgagac gttctcgccc
     4561 ctgtacgtcg cgatggtgcg cgcgggcgag gagagcggtg gcatggtcga cacgctccgc
     4621 atgatatcgc tccagctcga gcgctcgagc cagctacgga agaagatcaa gggcgcgatg
     4681 acgtacc
//
LOCUS       DASTSP010000033        23049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_741445, whole genome shotgun sequence.
ACCESSION   DASTSP010000033 DASTSP010000000
VERSION     DASTSP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 23049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23049
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741445"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFS75_01330"
     CDS             complement(<1..280)
                     /locus_tag="VFS75_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU4677341.1"
                     /translation="MDIVSSAVLGLVQGVTEFLPVSSTGHLVLTHALLGVENSSSLAF
                     DAVLQLATALAVIVYYFDEIYVLVQTVLRKLGRMPVNEKDYVMAKAIAI"
     gene            371..1165
                     /locus_tag="VFS75_01335"
     CDS             371..1165
                     /locus_tag="VFS75_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU4677342.1"
                     /translation="MPEQEPVQSTSPAPAAAPLRPRSSLGIPIAIVVSALLIAGAIVY
                     TGMNRPTTTAPVAGVNDPNATTPNPEPVTKNDHIRGNPNAPIVLIEYSDYDCPFCRLF
                     HDTMTKVMSTYGADGKVAWVYRHFPLAQLHPNAPKISEAAYCVAELGGNDAFWKFTDA
                     LNDSRKVTYDANGQIQAIEPTDMTRMDEFAKAGGVDTAKFESCYTSGKYAGQVQEDVA
                     AAMKAGARGTPYTVVVVGDQMSVINGAQPYESVKATLDTLLSQVGG"
     gene            complement(1162..4161)
                     /locus_tag="VFS75_01340"
     CDS             complement(1162..4161)
                     /locus_tag="VFS75_01340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU4677343.1"
                     /translation="MDHERFEEAYGRLNAAQREAVDTIEGPVMVVAGPGTGKTQILTL
                     RIANILKKTDTAPENILALTFTESGARAMRERLASYIGAPAYRVHVHTFHEFAGRCIR
                     EFPDAYEKRVGGRPVTDLEKVTMLETILETPSLTLLRPSGNPGFYIGPIVSALSLMKR
                     EYITPETFREIIAADEAVLAATPKVHEKGAHKGKVRSEYIEREKRLGRSRELLFVYET
                     YEALLGERGFFDYDDMIFETVRALEGNEDMLRALQETYQYVLADEHQDVNGSQNRILE
                     LLASFHERPNLFVVGDEKQSIYRFQGASLENFLYFGETYPHTKTIALTENYRSAQNIL
                     DLAHELISVDAGAAKDLRVPLTAAGKATGSIERRNFSHEAVEHDAIVAKIGELILKDV
                     PREEIAVIVRTNGEVEELSRYLRAHGIPAEASADGDILAHPITRSVRMLLGAAIDPAN
                     ESCLFEVLQSGFSDVPSGDLALAMRRVSWSRPLRHVIEDEATLRECGVVNPAPFVRLA
                     GVLRDARERMGIEAPHRILERLIRESGLLDHAVTIDPYEGSRVIRRLYDEVEEMVRRG
                     EAETLAAIERMFKTRVAHNLPLSAPYVRANRRSVEVMTAHKAKGLEFRHVFIPHLTDS
                     KWGGRARADLFRIPVARRLKDDVGPEDDERKLLYVAMTRAKEGLYLSSSGTSTEGRSL
                     LPTRLLEDLGEDLITEVDTAKEEETFDPAHALSATLARVPFDPALLIETLRERGLSVT
                     ALNNYLRSPWNYFYRNVLRIPEVEAESLLFGTALHDTLESVMKHRRENGALPSASELS
                     AYLEREFSKLPLTNEEYVRNHERGLTALSNYLAHVGPALPVETKEEYKIDAVIETGVP
                     ELPTLRLTGMLDRLDFDETGKLVRVVDYKTGKPKTRGYIEGTTKDSTGDYKRQLVFYA
                     LLLSLSGDERLQCRDGVLSFIEADEKGRIHEEAYTVTDEEMADLKTLIQRVVADIAKG
                     AFLDAPCDPAVCDYCELVAEQRRRTAA"
     gene            complement(4186..4569)
                     /locus_tag="VFS75_01345"
     CDS             complement(4186..4569)
                     /locus_tag="VFS75_01345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU4677344.1"
                     /translation="MEAPMDTNEEFSLVRRAASFTHAGRGIKVFVRSTHNAWIHLVVL
                     ALAIALGLFFDITTTEWIAIVFAGGFVLAAEAFNTAIEIDIDLTSPEYHPYAKDTKDV
                     AAGAVLIAAVTAAIIGALVFVPYLI"
     gene            complement(4614..4997)
                     /locus_tag="VFS75_01350"
     CDS             complement(4614..4997)
                     /locus_tag="VFS75_01350"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEU4677345.1"
                     /translation="MTNTPGERYLDTWVHIFNFYGCAEEAILRARKETIDAMVADAGF
                     GLLGSILSVFPGEASGYTYVAAISESGVDIHTWPEYGMSAQFEIHYCNYSRDNDDKAK
                     RLMEAFEAYFKPEKTEYIPPIAFPL"
     gene            5133..5588
                     /locus_tag="VFS75_01355"
     CDS             5133..5588
                     /locus_tag="VFS75_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4677346.1"
                     /translation="MEMRDITKDAVIISEDASFREAIELMVKKKTNTLLVVNEEGELS
                     GEVNVSDLLDAIIPMDLNPDNIDAVLGTEDGFKKAVRGAEDRTVMDFMSADFEAVHMT
                     DTLIEIAGTAIAHQTEHIPVVDRDNHPIGVISRQGLKHILAKYLGIKGE"
     gene            complement(5644..5781)
                     /locus_tag="VFS75_01360"
     CDS             complement(5644..5781)
                     /locus_tag="VFS75_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677347.1"
                     /translation="MRRDTLISRYPRLRRLGRRSAMRSVGRRIRCIDADRVMRELNYG
                     T"
     gene            complement(5791..6057)
                     /locus_tag="VFS75_01365"
     CDS             complement(5791..6057)
                     /locus_tag="VFS75_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677348.1"
                     /translation="MFSFVYSLFGVRQQSYRVSDRVELMVNGRFPKPKRQYIFILKRR
                     YTFFNGGIERVWVYIGPVYEPDGERIRCVHTEWRVPESALAARV"
     gene            complement(6180..6440)
                     /locus_tag="VFS75_01370"
     CDS             complement(6180..6440)
                     /locus_tag="VFS75_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677349.1"
                     /translation="MTYEQAAQYVGIAVAVTVIMREFGTRRIRTLSAADADALPAEQM
                     GGVRVKRLAKSRFNPHRGRGEKRKTFRDGEGTRTIRRFEYRF"
     gene            6652..7044
                     /locus_tag="VFS75_01375"
     CDS             6652..7044
                     /locus_tag="VFS75_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677350.1"
                     /translation="MPREPGFKKIRGERYWCPWTFDHLGGKRPDEHPPVERVFPKHGR
                     YRIVGVPVLAPGQVWVEDVGYLVVVLGYSEERREVTFRYYKCPLGLVVTRPFHLFRKH
                     FKVWEDAVRSRAIIRATYHRMLAAHGLA"
     gene            7125..7439
                     /locus_tag="VFS75_01380"
     CDS             7125..7439
                     /locus_tag="VFS75_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677351.1"
                     /translation="MSAQIIDFAKHQRVAARRRLSLVPLTLVKYPEKRPGSIPRDLFG
                     HPILTEGQYWKQGKNAIHLVRIERIADDKVTLRTFDSDFETTITADQFLRMYTLWDDV
                     PD"
     gene            7524..7970
                     /locus_tag="VFS75_01385"
     CDS             7524..7970
                     /locus_tag="VFS75_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677352.1"
                     /translation="MAYYGQLAKVRSNPPGGMRDGVPQAKLRGKWSATKGHGSAADPL
                     VRQTDLYGRYDPAKKEYVPYTFEELNGGAPRKDPKRVKYDERGQVIIEFGRPWKEKRT
                     HLLVAVGGMYEGGTMVAVRAWNADLTFRRTMSVYDFRDRFVPYDLT"
     gene            complement(8408..8800)
                     /locus_tag="VFS75_01390"
     CDS             complement(8408..8800)
                     /locus_tag="VFS75_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677353.1"
                     /translation="MFEDDDRILYLKTKVRRARKPHRCVECKRVITKGETYHFNYYAY
                     VPHRATRPYFESAKMCDECHVDWEILLKAELRESVFATHKCYGELRNRVLRACEDRWL
                     TEPEEVRLYVRWAWGRTLRLSKQIPLPY"
     gene            complement(8809..8976)
                     /locus_tag="VFS75_01395"
     CDS             complement(8809..8976)
                     /locus_tag="VFS75_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677354.1"
                     /translation="MSDSYSAGKAAEEAAKIKASRCNKSPNGAHAFEKRGSGPSRWEE
                     CRHCKTPIYYK"
     gene            complement(9070..9447)
                     /locus_tag="VFS75_01400"
     CDS             complement(9070..9447)
                     /locus_tag="VFS75_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677355.1"
                     /translation="MFRNAVLVLNESIVACESSEPYRCVECEQRFLAGEEHLVVERLV
                     LSAITGRVGTMVNVICHSCDADWRRILEVEFRIASTRRQRQYGLLRKRVVDACGKGGY
                     VKDEEADMFLRWRHGTVERERAL"
     gene            complement(9552..9734)
                     /locus_tag="VFS75_01405"
     CDS             complement(9552..9734)
                     /locus_tag="VFS75_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677356.1"
                     /translation="MLLWSFIAFWVLYGLIAVALEYFNFCAPHPLLWPFYVVQSFLDN
                     RRRERMLRDYPYRPPK"
     gene            complement(9744..10040)
                     /locus_tag="VFS75_01410"
     CDS             complement(9744..10040)
                     /locus_tag="VFS75_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677357.1"
                     /translation="MNDLSKFGLMVRAVRGCPKGQKRYEVRTANSNAFVALVHLTVRV
                     SDGEPVTTVATRLVERKEVEVGTQFVHRRELTEEQIAAIQEWQRSFVLPALSRA"
     gene            10160..10414
                     /locus_tag="VFS75_01415"
     CDS             10160..10414
                     /locus_tag="VFS75_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677358.1"
                     /translation="MKALYQEWKIESFRVGEGRLRSISKDLAQYEAEVAHQRTLENGA
                     VPDGDALIEVEVDDESAFPEMLENGTVFLHYDELDVLTFP"
     gene            complement(10457..10792)
                     /locus_tag="VFS75_01420"
     CDS             complement(10457..10792)
                     /locus_tag="VFS75_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677359.1"
                     /translation="MSKFLVMYLAPSATMDEWMKKPQEERADAEKKMMEDWNAWMAAH
                     KDLIKETWAAGKTKKVTGDGVSDSRNDLMLYSIVEAEDHDAAAKAFEGHPHFGIPGAT
                     IEVVAIRPM"
     gene            11089..11991
                     /locus_tag="VFS75_01425"
     CDS             11089..11991
                     /locus_tag="VFS75_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016152726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEU4677360.1"
                     /translation="MANWNQISEDLRTRGSTFDIIRKEALNKLHRYTKRNVIVYYSGW
                     LQKNSNELQQALSINDMDMNGFMTAINGLDSTVGLDLILHTPGGEVAATEAIISYLQS
                     KFQHIRVIVPQLAMSGGTMIACSAHEIIMGKHSSLGPVDPQIGGVPAHGIVEEFDKAH
                     KEIQADPSKLAVWQFVLQKYPPNFVGECQKAIDWSVKIIERNLRDRMLRGKADIDSIV
                     ARIKEELCNHAVSLSHSRHLPADKCREIGLEVIYLEDDQKLQDAVLTVHHATMQTIFN
                     TPAFKIIENHNGQAFILGTPQSHS"
     gene            complement(12123..13388)
                     /locus_tag="VFS75_01430"
     CDS             complement(12123..13388)
                     /locus_tag="VFS75_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4677361.1"
                     /translation="MAKRASSQKKKKKITSPRKKATNKRAKHAPKKKAIRKPAKAAKK
                     QVKKVAAKKPLLRPKKKLSRAEQAMEDKAVALLAVGRGRGYVTYDEILREFPDIEKKV
                     GFLEDLYERFATASIDVIGSGGMLGDDIDDTLLDRRNVYKRSESSYDSIQMYLREIGQ
                     YPLLTAQEERALAKRIVEGDSEARNLLARANLRLVVSIAKKYVGRSPDLTLLDLIQEG
                     NLGLFKAVDKFDFTKGFKFSTYATWWIRQAITRALADQSRTIRIPVHMVETMAKYKQV
                     SRRLAQDLGREPLAEEIATEMDVEVDKIYQIEKISQDTISLELPIGDDDDRSRLSDFI
                     ADDKIIAPDQEVAHRILADQMQEILGSLSEKERKILEMRHGLLDGTYHTLEEVGKEFG
                     VTRERIRQIEAKALEKIRQHEKARRLKSY"
     gene            complement(13706..15448)
                     /gene="dnaG"
                     /locus_tag="VFS75_01435"
     CDS             complement(13706..15448)
                     /gene="dnaG"
                     /locus_tag="VFS75_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU4677362.1"
                     /translation="MRSPVEEIKERLNILDIIGQYVELKRAGKSYKGKSPFTNEKTPS
                     FYVSPDRGMYYCFSSQQGGDMFTFIEKMEGVDFKGALKILAEKAHVELVPEDPKKRDA
                     RETAYALLEEATRYFEENLREAPEIRKYVEERGVTGATIQSFRIGYAKDEWRALRAHL
                     KEKGFTDEALLAAGLAKRAEGKEPYDVFRDRVMFPIMDPSGRVVAFSGRTRKAEEGVP
                     KYVNSPETDLFQKSEILYGYDKAKHGIRSMNFALIVEGQFDLVLSHQAGYVNAVAVSG
                     TALTPHHVGLLTRISKNVVLSLDADRAGITAVKRSAGPMLEAGMDVKVAELPEGEDPA
                     DVVRKDPKRLKAIVGKSVHVVEFLLKTLKSNAKDERTYKLRVREEVLPLLSSMDNRID
                     REHFEGVVAEYIESTKEGVHFEVERIAEAKKHERRAAVAVVAKKEKEEKGIEPNRKKT
                     LEDRIQAYMHIAAGERAWLGKRIREELEKVLGDDAMALFASAPSAEATALIFRLEEDE
                     ENLRERELLEQVSDALTQYMHAIARERIRGIKVQVREAEESGKGDDALLLLKEAKAYE
                     KFLASSIVLTKEGE"
     gene            complement(15547..19194)
                     /gene="rpoC"
                     /locus_tag="VFS75_01440"
     CDS             complement(15547..19194)
                     /gene="rpoC"
                     /locus_tag="VFS75_01440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU4677363.1"
                     /translation="MKTNQQFTEVALKLASPDRILEWSKGEVTKPETINYRTQRPEKH
                     GLFDERIFGPVEDYECACKKYRGIRYKGIVCEKCGVEVTRSIVRRERMGHIELCVPVS
                     HIWFLRGVPSRIALILGTSSASIEKVIYFAGYIVTKVSSDERQRILKELDTEFKTKYK
                     ELQTEQAREELKNRMDDIKSEIESITEGTILDEMHYHKYSMKYSTLFEARIGAEAIYD
                     IFKKLDLVELQARLEKERETAGAAERAKLDKRLSLIKGMIHAGVRPEWMFLTRIPVIP
                     PALRPMVALEGGRHASSDLNDLYRRVINRNNRLKKLMDIMAPDVILRNEKRILQEAVD
                     ALIDNSIRHGNTAYSAMSQAQRRPLKSLADYLKSKQGYFRQNLLGKRVDYSGRSVIVI
                     GPDLALDQCGLPKHMALELFRPFVISELLKQELAYNIRGAGRLIEDGVPEVWAILEDV
                     IRDRHVLLNRAPTLHRQGIQAFRPVLIEGNAIQVHPLVCRAFNADFDGDQMAVHVPLS
                     DEAQFEAREIISANKNILKPGSADPVVSEKLLDMALGAFWMTKVMEGAKGEGKFFSSP
                     NAAILAYDFGVLDIRAKVKVLATDTPKYKQFEGKVFETTVGRLLFNSVLPSEHPFIND
                     VITQRTLFGIIVDLIDEEGIDAVPRIVDRMKRFGFEYATKSGTTWGIDDVVVPEVKQS
                     IIAKAREEEAKVFGFFNDGLISHEERRRMIIEIWHKAKTEIEQALPATLDENGSVFDM
                     WKSGARGSMGQIAQMAGMKGLIINTRGETLEFPILSSMKEGMSPIEYFITTHGSRKGL
                     ADTALQTAKAGYLTRRLFVVAQDAIVTEDDCKTKEGVTIGRISSSGIEIAFSKAIRGR
                     ILAADAVTKDGTVMFKKGHLLSRLDSIAIEKSDIESVEVRSPMTCKTLQGVCQQCYGI
                     DLTTNKLVDIGEAVGTVAAQAIGEPGTQLTMNTKHAGGAASVGGDVTQGLPRVEEVFE
                     KRAPKIPAVISRTDGVVTNIRTEGKEKVITVAPAVGSKYATKKNDNIEYAVHFRRVIT
                     VSVGDSVKQGQLMTDGSAHLPDLFKYAGQEATQEYIINETNKIYELQGVTIARKHIEL
                     IVKQMMSRVKITQAGESSFTVGDVVEEWNLARVNDELKAAGKEPAKGERLILGITETS
                     LSRKSFLSAASFQNTTRVLINAAVRGSEDKLTGLMENVIIGRLIPAGTGFKGSRKWDA
                     IKQFENDPVEE"
     gene            complement(19216..22416)
                     /locus_tag="VFS75_01445"
     CDS             complement(19216..22416)
                     /locus_tag="VFS75_01445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU4677364.1"
                     /translation="MSNAPKAKENEGAVLPKKHFQRFRDARVDLPDLVEPQRESYKNF
                     IEVGLKEVFKEFSPISDYSEKKFDLIFKKYELGSPKTTPEAAHENKLTYEAPLRAQVV
                     LKNKTFGSEKEQEIFLADLPVMTENGTFIINGVERVIVPQLARSYGIFFTTTESKGRT
                     YFGAKIIPARGAWVEIESEADGVIYVKIDRKRKFPISSLFRVFGVTKDADMVKLFKGV
                     EHADEYMKRTIAKDVAKTLDDSYIEIYKRLRDGDLATVENAKEFVDSIFSPLRYDLSE
                     IGRHHFNERFGLKTDAKADAQRTVTTEDLVTILKNIIESNHTPGAIADDIDHLGFRRV
                     RYFGEMLQQRIRVGMTRVKRNIQDRMSTIEAETSLPIQLVNPRPLQAAIKEFFTTNQL
                     SQFMQQQNMLAELEGLRTLSALGPGGLTRERASFEVRDVHPSHYGRLCPIHTPEGPNI
                     GLILRLSMYARVNRFGIIETPYAKVEKGVVTDEIVYLNAHEEEKYNIAHAAVEVDDNG
                     KIKPETLEVRYHGEPHSVSRKEVDYIDIATNQAFSVATSMIPFINHDDANRALMGSNM
                     QKQATPCIVQEAPFVATGVEELAARDTGRMIVAEEAGEIVEVDARHIVLKNKDGKKKE
                     YALTTFQRTNDFSAFYHRAAVSVGQKVKRGDILANTSSTENGQMALGQNMLVAFMSWS
                     GANYEDAIIISERVVKDSKFATIHLDELEVSVRDTKLGPEVTTPDIPNVSELKLRNLD
                     EEGIIRIGAEVRPGDILVGKVTPKGETQLTPEERLLRSIFGEKAKDVKDTSLRLEAGK
                     RGRVIGVKIFSREAGDQLESGVIKRIHITVAQVRNVSVGDKLAGRHGNKGVISRILPE
                     EDMPYTADGRPVDVILTPLGVPSRMNLGQILELHLGMAANTLGYQAIVPPFAGAGEKE
                     VKEELKAAGLPEDGKLWLKDGRTGDMFAQRISVGYMYILKLHHMVEDKIHMRSIGPYS
                     LITQQPLGGKAQVGGQRFGEMEVWALLGYGAAYTLREMLTIKSDDIVGRSAAFDAIVR
                     GEKISHPHTPAAFNVLVNQLRGLALDIDLRREEDRI"
     gene            complement(22866..>23049)
                     /locus_tag="VFS75_01450"
     CDS             complement(22866..>23049)
                     /locus_tag="VFS75_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU4677365.1"
                     /translation="ARHIPGVLGTFESLEDERISSGVMYTRPEVLIHKGKKYKVPPIL
                     TKGNHAEIEAWKRSQA"
BASE COUNT         4314 a         7014 c         6989 g         4732 t
ORIGIN      
        1 cgatggcgat cgccttcgcc atgacatagt ccttctcgtt gaccggcatg cggccgagct
       61 tccgaaggac cgtctgcacg aggacgtata tctcgtcgaa gtagtagacg atgacggcga
      121 gcgcggtcgc gagctggagg accgcgtcga acgcgagcga gctcgagttc tcgacgccga
      181 ggagcgcgtg cgtgaggacg aggtgaccgg tcgacgaaac gggaaggaat tccgtcacgc
      241 cttggacgag gccgaggacc gcgcttgaga cgatatccat ggcggaagta tagcatgggc
      301 gcaatatcgc tatggtatac tttcggggta tttaccacgg ggcgagaccc cagataagca
      361 ttcaaaacgc atgcctgaac aagaaccagt acagtcgacg tcgccggctc ccgcggcggc
      421 tcccctccga ccgcggagtt cgctcggcat cccgatagcg atcgtggtct cggcgctcct
      481 catcgccggc gctatcgtct acacgggcat gaatcgtccg acgacgaccg ctccggtcgc
      541 cggagtgaac gacccgaacg cgacgacgcc gaacccggag cccgtcacga agaacgacca
      601 catccgtggc aatccgaacg cgccgatcgt cctcatcgag tactccgact acgattgtcc
      661 gttctgccga ctcttccacg atacgatgac gaaggtcatg agcacgtacg gcgcggacgg
      721 caaggtcgcg tgggtgtatc gtcacttccc tctcgcccaa ctccacccga acgcaccgaa
      781 gatctccgag gcggcgtact gcgtcgccga actcggcggc aacgacgcct tctggaagtt
      841 cacggatgcg ctcaatgatt ctcgaaaggt gacgtacgac gcgaacgggc agatacaggc
      901 tatcgaacct accgatatga cccgcatgga cgagttcgcg aaggcgggcg gcgtcgacac
      961 ggcgaagttc gagtcctgct acacgagcgg caagtacgct ggccaagtgc aggaagacgt
     1021 cgcggccgcg atgaaggctg gcgcacgcgg caccccgtac acggtcgtcg tcgtcggcga
     1081 ccagatgtcc gtcataaacg gcgcacagcc gtacgagtcg gtgaaggcga ccctcgatac
     1141 gctcctctcg caggtcggcg gctaggccgc cgtccgtcgt cgctgttcgg cgacgagctc
     1201 acaatagtcg catacggcgg gatcgcaagg cgcgtcgagg aacgcgcctt tcgctatgtc
     1261 cgcgacgacg cgttggatga gcgtcttcaa gtccgccatc tcctcatccg tcacggtgta
     1321 cgcctcctca tggatacgtc ccttctcgtc cgcctcgatg aacgagagaa cgccgtctcg
     1381 acactgaagt cgttcgtcgc ccgagagcga gaggaggagc gcgtagaaga cgagctgacg
     1441 cttgtagtcc cccgtcgaat ccttcgtcgt gccttcgatg tagccgcgcg tcttcggctt
     1501 ccccgttttg taatcgacga ccctaacgag cttccccgtc tcgtcgaagt cgagcctatc
     1561 gagcataccg gtgaggcgga gcgttggaag ctcgggaacg cctgtttcga tgaccgcgtc
     1621 tatcttgtat tcctccttcg tctcaacggg gagcgcgggg ccgacgtgcg cgaggtagtt
     1681 cgagagggcg gtgagaccgc gttcgtggtt gcggacgtac tcctcgttcg tgagcggcag
     1741 tttcgagaat tcgcgctcga ggtacgcgga aagttctgac gccgacggca gtgcgccatt
     1801 ctctcgccga tgcttcatga cgctctcaag ggtgtcgtgg agtgccgtgc cgaagagaag
     1861 gctttccgcc tcgacctcgg ggatgcggag gacgtttcta tagaaatagt tccaggggct
     1921 ccggaggtaa ttgttgagcg cggtcaccga gagcccgcgt tcgcgaagcg tctcgatgag
     1981 gagcgcgggg tcgaacggca cgcgcgcgag ggtcgccgag agcgcgtgcg ccggatcgaa
     2041 ggtctcctcc tccttcgccg tgtcgacctc ggtgatgagg tcctcgccga ggtcttcgag
     2101 gagacgcgtc gggaggagcg agcggccttc ggtgctcgta ccggacgacg agaggtagag
     2161 cccctccttc gcgcgcgtca tggcgacgta gaggagcttc cgctcgtcgt cctccgggcc
     2221 gacgtcgtct ttgaggcgcc tcgcgacggg gatgcggaag aggtccgcgc gagcgcgacc
     2281 gccccacttc gagtcggtga gatgcggaat gaacacgtgt ctgaactcga ggcccttcgc
     2341 cttgtgcgcc gtcataacct cgaccgaacg ccgattcgcg cgaacgtacg gtgcgctaag
     2401 cggaaggttg tgcgcgacgc gggtcttgaa catgcgctcg atcgccgcga gcgtctccgc
     2461 ttccccgcgc cggaccatct cctcgacctc gtcgtagagg cggcggatga cgcgactgcc
     2521 ctcatagggg tcgatggtga ccgcgtggtc gagaagaccg ctctcgcgga taaggcgctc
     2581 gaggatgcga tgcggcgcct cgatgcccat acgctcgcgc gcgtcccgaa ggacacccgc
     2641 gaggcgtacg aagggcgcgg gattcacgac accgcactcc cggagcgtcg cctcgtcctc
     2701 gatcacgtgt cgaagcgggc gactccatga gacccgccgc atcgcgagcg ccagatcgcc
     2761 cgagggcacg tccgagaaac ccgactggag gacctcgaag aggcacgatt cgttcgccgg
     2821 gtcgatggcc gcgccgagaa gcatgcggac gctccgcgta atggggtgcg cgaggatgtc
     2881 accgtccgcg gaggcttctg cgggaatgcc gtgcgcgcgg aggtaccgtg agagctcctc
     2941 gacctcgccg ttcgtccgca cgatgaccgc tatctcctcg cgcggaacgt ccttcaggat
     3001 gagctcgcct atcttcgcga cgatggcgtc gtgctcgacc gcctcatggg agaagttccg
     3061 ccgctcgatc gagcccgtcg ccttccccgc cgcggtaagg ggaacgcgaa ggtccttcgc
     3121 ggcacccgcg tcgaccgaaa tgagctcatg cgcgaggtcg aggatgttct gcgcggagcg
     3181 atagttctcc gtaagcgcga tggtcttcgt gtgcgggtac gtctcgccga agtagaggaa
     3241 gttctcgagc gaggcgccct gaaaacggta gatcgactgc ttctcatcgc cgacgacgaa
     3301 gaggttcgga cgctcatgga acgacgcgag aagttcgagg atgcggtttt ggcttccgtt
     3361 cacgtcctgg tgctcgtccg cgaggacgta ctggtacgtc tcctggagcg cgcggagcat
     3421 gtcctcgttc ccctcgagcg cacgcaccgt ctcgaatatc atgtcgtcat agtcgaagaa
     3481 cccacgctcg ccaaggagcg cctcgtacgt ctcgtacacg aagagaagct cgcggctccg
     3541 cccgaggcgc ttctctcgct cgatgtactc gcttcgcacc tttcccttgt gcgcgccctt
     3601 ctcgtgtacc ttcggcgtcg ctgcgagcac cgcctcgtcc gcggcgatga tctcgcggaa
     3661 ggtttcgggc gtgatgtact cgcgcttcat gagggagagc gccgagacga tcgggcctat
     3721 gtaaaaaccg ggattccccg agggccgaag gagcgtgagc gatggggtct cgaggatcgt
     3781 ctcgagcatc gtcaccttct caagatcggt gaccggtcgg ccgcccacgc gcttctcgta
     3841 cgcgtccgga aactcgcgaa tgcagcgacc cgcgaattca tggaacgtat gaacgtggac
     3901 gcggtacgcg ggagcgccga tgtacgatgc gaggcgctcg cgcatcgcgc gagcgcctga
     3961 ttcggtgaaa gtgagcgcga ggatgttctc gggcgcggtg tcagtcttct tgaggatgtt
     4021 cgcgatcctc agcgtgagta tctgcgtctt ccccgtcccc ggccccgcga cgaccatgac
     4081 cggcccctcg atggtatcga cggcctcgcg ctgtgcggca ttcagcctcc cgtaggcctc
     4141 ctcgaagcgt tcgtggtcca tggggcccat tatacgcgag ggcggctaaa tgaggtacgg
     4201 gacgaacacg agggccccga tgatggccgc ggtcacggcc gctatgagga cggcacccgc
     4261 ggcgacgtcc ttcgtgtcct tcgcgtacgg gtggtactcg ggcgacgtga ggtcgatatc
     4321 tatctctatc gccgtattga acgcttcggc cgcgaggacg aagccgcccg cgaagacgat
     4381 agcgatccat tcggtcgtcg tgatgtcgaa gaagaggccg agcgctatgg cgagcgcgag
     4441 cacgacgagg tgtatccacg cgttatgggt cgagcgcacg aagaccttga taccgcgacc
     4501 ggcgtgcgtg aaactcgccg cccgcctcac gagggagaat tcttcattcg tatccatcgg
     4561 cgcttccatg cgaaaagtat agcacctcga aaaaaccgcc cgaaggcggt tctctagagg
     4621 gggaacgcga tgggcggtat gtattcggtc ttctcgggct tgaagtacgc ctcgaacgcc
     4681 tccatgaggc gcttcgcctt atcatcgttg tcgcgcgagt agttgcagta gtgtatctcg
     4741 aactgcgcgc tcatgccgta ttccggccac gtgtggatat cgacgcccga ttccgatatc
     4801 gcggcgacgt acgtgtatcc cgaggcctca ccgggaaaga cgctcaagat ggagccgagg
     4861 agcccgaagc cggcgtcggc gaccatcgcg tcgatcgtct ccttccgcgc gcggagaata
     4921 gcctcttcgg cgcacccgta gaaattgaag atatggaccc acgtgtcgag atatcgttct
     4981 ccgggagtgt tcgtcatttc ttttttagaa tgataacgac tctcgccgaa aatgcaatgc
     5041 cgggcgcggt agaaaaagga cttcacgcgc tcttcatgac gaatcggcga tagtgatgtt
     5101 tacaacgaga gccggcatag tatcattaac taatggaaat gcgcgatatc acaaaggacg
     5161 ccgtgatcat cagcgaagat gcctcctttc gcgaagcgat cgagctcatg gtgaagaaga
     5221 agacgaacac gctcctcgtc gtgaacgagg agggcgaact ctccggcgag gtgaacgtct
     5281 ccgaccttct cgacgcgatc atccccatgg acctgaaccc ggacaacatc gacgccgttc
     5341 tcggcaccga ggacgggttc aagaaagcgg tccgcggcgc cgaggaccgg accgtcatgg
     5401 atttcatgtc cgccgatttc gaggccgtcc acatgacgga cacgctcatc gagatagcgg
     5461 ggacggcgat cgcccaccag accgagcaca ttcccgtcgt cgaccgagac aatcatccca
     5521 tcggcgtcat ctcgcgccag ggcctcaagc acatcctcgc gaagtacctc ggcatcaagg
     5581 gcgagtagcc cgccggcgaa catgaaaaac gggcgagtct tcgactcgcc cgttcgcgtg
     5641 tgatcacgtg ccatagttca attcccgcat gacgcggtcg gcgtcgatgc accggatccg
     5701 tctcccgacg cttcgcatcg cacttcgccg tccgagtcgc cttaaccgcg ggtaccgtga
     5761 gatgagcgta tcgcgccgca tgccgttctc ctatacgcgc gccgcgagcg ccgactccgg
     5821 aacgcgccac tccgtgtgga cgcaccgtat ccgctcgccg tccggttcgt acaccggacc
     5881 gatgtagacc cagacgcgtt cgataccgcc gttgaagaac gtgtacctcc gtttcaaaat
     5941 gaagatgtac tgtcgcttcg gtttcgggaa tcgcccgttc accatgagct cgaccctatc
     6001 cgagacccta tagctttgct gtctgacgcc gaaaagcgaa tagacgaacg agaacatggc
     6061 gaactccctt ccgtcgttgt gccgaagaaa gcgtagcaga gttcgagacc gcgggcaaca
     6121 aaaaggggcg ggagcgaatt gcgttcgcgt gtctcccgcc ccttgtgcgc tccctgctgt
     6181 tagaaccggt actcgaagcg ccggatggtc cgggtgcctt cgccgtcgcg gaaggtcttc
     6241 cgcttctcgc ccctgccgcg gtgcggattg aagcggctct tcgcgaggcg cttcacgcgc
     6301 acgccgccca tctgctccgc ggggagcgcg tccgcgtccg ccgcactaag cgtgcggatg
     6361 cggcgcgtgc cgaactcccg catgatgacg gtgacggcga ccgcgattcc cacgtactgg
     6421 gccgcttgct cgtacgtcat agacctgagc atatgtactt cctccttatt gatgtggagt
     6481 ctgcgagcct tcgctcgcga tcggaactac ctattatagt aggacacatt atgtgtattg
     6541 tcaatgggcc agtggcgtgc tagcatgcgg tcagatcacg ctccgaaagg accgaccatg
     6601 gtacgtatct accgtttcag caatcggcgt cctccctgcc cggaggacgg catgccgcgg
     6661 gagcccggct tcaaaaagat ccgcggggag cggtactggt gtccctggac gttcgaccac
     6721 ctcgggggga aacggccgga cgagcaccct cccgtcgaac gggtgttccc gaagcacgga
     6781 cgctaccgca tcgtcggcgt tccggtgctc gcgcccggac aggtctgggt cgaggacgtc
     6841 ggctacctcg tcgtcgtgct cgggtactcc gaggaacgcc gcgaggtcac gttccgctat
     6901 tataagtgtc cgctcgggct cgtcgtcacg aggcccttcc acctcttccg gaagcacttc
     6961 aaggtatggg aggacgcggt gcgctcacga gctatcatcc gcgcgacgta ccaccgtatg
     7021 ctcgcggcgc atgggctcgc gtgagatgcg gcgattcggc cgacccttcg ggtcggccgt
     7081 tcttttatgc tacgatacgg caagtaccgt tcacggagaa cagaatgagc gcacagatca
     7141 tcgacttcgc gaagcaccag cgcgtggcgg cgcggcgacg actgtcgctc gtcccgctca
     7201 ccctcgtgaa gtatcccgag aagcgtccgg gttctattcc ccgcgacctc ttcggtcatc
     7261 ccatactcac cgaggggcag tactggaaac aggggaaaaa cgccatacac ctcgtccgca
     7321 tcgaacgcat cgctgacgac aaggtcacgc tccggacgtt cgattcggac ttcgagacga
     7381 ccatcacggc agaccaattc ctccgcatgt acacgctctg ggacgacgtc ccggactgac
     7441 gcgcgtgaag ccaacctcgg ttggcttttc ttttgccctg tgctaggatt tcggccggca
     7501 cgttcatcaa cacggaggaa ccgatggcct actatggtca gctcgcgaag gtccgcagca
     7561 acccacccgg tggcatgcgg gatggggttc cccaggcgaa gctccgcggc aagtggagcg
     7621 ccacgaaagg acatggctcc gcggccgatc ctttggtccg gcagaccgac ctctacggcc
     7681 ggtacgaccc ggcgaagaag gagtacgttc cgtacacgtt cgaggagctg aacggcggcg
     7741 cgccgaggaa ggatccgaag cgcgtgaagt acgacgaacg cggccaggtc atcatcgagt
     7801 tcggccgccc gtggaaggag aagcgcacgc atctcctcgt cgccgtcggc ggcatgtacg
     7861 agggcggcac gatggtcgcc gtgcgcgcct ggaacgccga tctgaccttc cggcggacga
     7921 tgtccgtcta cgacttccgg gaccggtttg tcccgtacga tctcacctga cccgtcccag
     7981 ggtcctttgg gccgacgctc cccttcgtcg gccctttttt tatgcctgca caaccgaact
     8041 agaccatgcc gtaagacggg tgtacgatta cagacggttt tcgtagtcgt tcgttgtact
     8101 tccctagcgt tcacctacat cgcggtcaca ataagggaga cggtgatgca catgtatccg
     8161 catcagatgg cgaggttgga aagcgtttcc gccgaagagg gtcaggactc gacggcgcgg
     8221 cgtgatgaaa acaagcggtc cgcgaagacg gagccgcgcg gcacgagcag gcaggatctt
     8281 tcgttttgcg tacgcccgac ccccgaggtc aggcggcgct agcctcgcga ggcgcacatt
     8341 cgaagccgac cagagatggt cggctttttc attggcaaaa aataaaaggc ggtcgcatgc
     8401 gaccgcctta ataaggaagt ggtatctgct tggagagccg aagagtcctt ccccacgccc
     8461 aacggacgta gagtcgtact tcctcgggtt cggtcagcca ccgatcctca caagctctaa
     8521 gaacgcgatt tctgagttcg ccgtaacact tgtgcgtcgc gaacaccgat tcccgcagtt
     8581 cggctttgag aaggatctcc caatccacat ggcactcgtc gcacatcttc gccgattcga
     8641 agtacgggcg agtagcgcga tgggggacgt acgcgtaata gttgaagtgg tacgtctccc
     8701 ccttggtgat gacgcgcttg cactcgacgc acctatgggg ctttcgcgct cgccggacct
     8761 tcgtcttgag gtacaggatc ctgtcgtcgt cctcgaacat aggccatcct atttgtagta
     8821 gatgggcgtc ttgcagtgcc ggcactcctc ccaccgggaa ggcccggaac ctcgtttctc
     8881 gaaggcatgc gccccgttcg gagacttgtt gcacctactc gccttgatct tcgcggcctc
     8941 ttcagccgcc tttcccgcgg aatacgagtc gctcatggct gtcctcctcg atatctcgcg
     9001 ccattctggc acaccggcgg gcaaaaataa aggcggtccg tagaccgcct ccacatgtgg
     9061 acgcttcctt cagagcgccc gttcccgctc gaccgtcccg tgcctccagc ggaggaacat
     9121 gtcggcttcc tcatctttca cgtagccgcc cttcccgcac gcgtcgacga cgcgcttccg
     9181 gagaaggccg tactgccgct gccgccgcgt cgaagcgata cggaactcga cctcgaggat
     9241 gcgccgccag tcggcgtcgc acgagtggca gatgacgttg accatcgtcc ccacgcgccc
     9301 cgtgatcgcc gagaggacga gccgctcgac gacgaggtgt tcctcccccg cgaggaaacg
     9361 ctgttcacat tcgacacagc ggtacggttc gctcgactcg cacgcgacta tgctctcgtt
     9421 cagtaccaag accgcattcc ggaacatggc actctccctt cttgtgaaat gagaagaaca
     9481 gcaccttccc tataattgtc ccaccatcgg agaaagccgc aacgggcgaa agaggagaaa
     9541 aggaaaccgc cctacttagg agggcggtac gggtaatcgc ggagcatgcg ctctctccgg
     9601 cgattatcca ggaaggactg aacgacgtag aagggccaga ggagcggatg cggcgcgcag
     9661 aaattgaagt attcaagcgc gaccgcgatg aggccgtaga gcacccagaa ggcgatgaag
     9721 gaccagagga gcatggccgt ctcctacgcg cgcgagagcg ccggaagaac gaacgagcgc
     9781 tgccactctt gaatggcggc gatctgttcc tccgtgagct cgcgtcggtg gacgaactgc
     9841 gtcccgacct ccacttcctt tcgctcgacg agccgcgtcg cgaccgttgt gaccggctcg
     9901 ccgtcgctca cgcggacggt gaggtggacg agcgcgacga acgcgttcga gttcgcggtg
     9961 cggacctcgt accgcttctg ccctttggga cagccccgaa cggcgcgaac catgaggccg
    10021 aacttggaaa ggtcgttcac gttgttctcc cttgagttgt acgaaccggg tgaagcgtag
    10081 caaaaagcgg cggccgccgc aaacttgcca tctaccgtcc ccgtgatacc ctctttctag
    10141 gaaacccgag gagagcacca tgaaagcgct ctatcaggag tggaaaatcg aatcgttcag
    10201 agtcggtgag ggccgactcc gttcgataag taaggacctc gcgcagtacg aagcggaggt
    10261 cgcgcaccag cgaacgctcg agaacggcgc cgtgcccgac ggcgacgcgc tcatcgaggt
    10321 cgaggtcgac gacgagtcgg ccttccccga gatgctcgag aacggaacgg tcttcctcca
    10381 ctacgacgag ctcgacgtcc tcacgttccc ctgaaccaca aacggcttcg cttcgcgaag
    10441 ccgtttttac atgtccctac atcgggcgga tggcgacgac ctcgatggtc gcgcccggga
    10501 tgccgaagtg tgggtggcct tcgaacgcct tcgcggcggc gtcgtggtcc tccgcctcga
    10561 cgatcgagta gagcatgagg tcgtttcgag aatccgagac gccgtcaccc gtgactttct
    10621 tcgtcttccc cgccgcccat gtctccttga tgaggtcttt gtgcgcggcc atccatgcgt
    10681 tccagtcctc catcatcttc ttctccgcat cagcgcgttc ttcctgcggc ttcttcatcc
    10741 actcgtccat cgtcgccgac ggcgcgaggt acatgaccaa aaatttactc atggattaaa
    10801 aaattattgt agtaacaaga atagtaaatc agtataccaa acgcggttag cggcggaatt
    10861 agctataaca aattcattta aaaaaatgtc aatatgctat aatgctcgat atgccgaatg
    10921 gtactcctca gcgagccggc gaaaaacgcc ttacagaaaa cgaagtcgag caacttctaa
    10981 aaaaggtgcg tgagttccaa gaaacaaatc aaagatattt gataagacct gttgctcgtc
    11041 aggagtcaaa cactccgatt atcaatatgg ccaacaccaa tttctaacat ggcaaattgg
    11101 aatcagattt ccgaggacct tagaactcga ggttcaactt ttgatatcat ccgtaaagaa
    11161 gcgttgaata agttgcatcg atacacaaaa cgaaatgtca ttgtgtacta tagcggttgg
    11221 cttcaaaaaa actctaacga acttcaacaa gccctctcaa tcaatgatat ggatatgaat
    11281 ggtttcatga ccgctattaa cggtcttgat tctacagtag gtttagacct gattttgcat
    11341 acacctggcg gagaagttgc ggcaaccgaa gcgattattt cttatcttca atcgaagttc
    11401 caacacatac gtgtgattgt tcctcaactg gcgatgtcgg gaggaacaat gattgcctgt
    11461 tctgctcacg aaattattat gggcaaacat tctagtctcg gcccagtcga tccccaaatt
    11521 ggcggagtgc cagctcatgg gatagttgaa gaatttgata aagctcataa ggagattcaa
    11581 gcagacccat caaagctagc tgtgtggcaa ttcgtccttc agaaataccc accaaacttt
    11641 gtgggtgagt gtcaaaaagc cattgattgg tctgtgaaaa ttatagaaag aaatctgcga
    11701 gatagaatgc ttcgtggaaa agccgacatt gattcaatcg tcgcccgtat taaagaggaa
    11761 ctatgtaacc acgcagtaag cttatctcat agcagacatt taccggcaga caaatgtcgc
    11821 gagattggat tggaagtcat ttatcttgaa gatgatcaaa aattacagga tgcagtattg
    11881 acagtacatc atgctactat gcaaaccata tttaacacgc cggcattcaa aattatagag
    11941 aaccataatg gtcaagcctt tatcttgggt acaccccaaa gtcactcgta atctttggcg
    12001 aactcttcag gcggcgagtg agaaatgggc tgggacccat ttcgcaagag gttgtcgaaa
    12061 aagcccggcg ttcatgccgg gctttttcat acaacctgaa cagcgcctgt aaggcgtgaa
    12121 agctagtacg acttcagtcg tctagctttc tcgtgctgtc ggattttttc taaagccttc
    12181 gcctcgatct gtcggatacg ctcgcgcgtg acgccgaact ccttccctac ctcttcgagc
    12241 gtgtgatacg tgccgtcgag aagtccatgg cgcatctcga gaatcttccg ttccttctcc
    12301 gagagcgagc cgagaatctc ctgcatctgg tcggcgagga ttcggtgcgc gacctcctgg
    12361 tcgggcgcga tgatcttgtc gtccgcgatg aagtcggaga ggcgcgagcg gtcgtcgtcg
    12421 tcgccgatgg ggagttcgag cgagatggtg tcctgcgata tcttctctat ctggtatatc
    12481 ttatcgactt cgacgtccat ctccgtcgcg atctcctcgg cgaggggctc acggccaagg
    12541 tcctgcgcga ggcggcgcga gacctgcttg tacttcgcca tcgtctccac catgtgaacg
    12601 gggatacgga tggtgcgcga ctggtcggcg agggcacgcg taatggcctg gcgaatccac
    12661 caggtcgcgt acgtcgagaa cttgaagccc ttcgtgaagt cgaacttgtc gacggccttg
    12721 aagaggccga ggttcccctc ctgaatgagg tcgaggagcg tgaggtccgg gcttcggccg
    12781 acgtacttct tcgcgatcga gacgacgagg cggaggttcg cacgcgcgag gaggttacgc
    12841 gcctcgctgt cgccctcgac gatgcgcttc gcgagcgctc gctcctcctg tgcggtaagg
    12901 agcgggtact gccctatctc gcggaggtac atctggatgg agtcgtacga agactccgaa
    12961 cgcttgtaga cgtttcgcct atcgagaagc gtgtcgtcga tgtcgtcgcc gagcatgccg
    13021 cccgaaccga tgacgtcgat ggacgccgtc gcgaaccgct cgtagaggtc ctcgaggaac
    13081 ccgaccttct tctcgatgtc cgggaactcg cggagtatct cgtcataggt gacgtaaccg
    13141 cggccgcggc cgacggcaag gagcgcgacg gccttgtcct ccatcgcctg ctccgcacgg
    13201 gagagcttct tcttcggacg aagaagcggc ttcttcgccg ctaccttctt cacctgcttc
    13261 ttcgccgcct tcgcgggctt tcgtatcgcc ttcttcttcg gcgcgtgctt tgcacgctta
    13321 ttcgtcgcct ttttgcgggg cgacgttatc ttcttcttct ttttttgtga cgacgcgcgt
    13381 ttcgccataa tgaaacgtaa ctatacatta aaactcgaaa atgtccatat tcaaagcggc
    13441 gcgaggagac gccctccccg ggcgagagcc cggaaatcca ccgtttccta taactcacct
    13501 atttacaggg aagttcacct tccgtacact caccatgtcg gcgcacccct gcccgacccg
    13561 ccgagaggag gagacagccg ccatgcccaa ttcctcgcaa cgccccttcg gacacgtcgt
    13621 ccacggcctg cacctcgaag cacaacctcc gcccgcgtga cccccacgcg agcccacggc
    13681 cccatctccc tcggtggggc tttttttatt cgccttcctt tgtgaggacg atcgaggagg
    13741 ctaaaaattt ctcgtacgcc ttcgcttcct tgaggaggag gagcgcgtcg tcacccttgc
    13801 cgctctcctc cgcctcccga acctgaacct ttatgccgcg gatgcgctcg cgcgctatcg
    13861 cgtgcatgta ctgcgtgagc gcgtcggaca cctgttcgag gagctcgcgc tcgcggagat
    13921 tctcctcatc ctcctcgagg cggaagatga gcgcggtcgc ctcggcgctc ggcgccgagg
    13981 cgaagagcgc catcgcgtcg tctccgagga ccttctcgag ctcttcccgt atccgcttcc
    14041 cgagccacgc gcgctcgccc gcggcgatgt gcatgtacgc ctgaatgcgg tcctcgagcg
    14101 tcttctttcg gttcggctct atgcccttct cctccttctc cttcttcgcg acaaccgcta
    14161 cggcggcgcg acgctcgtgc ttcttcgcct cggcgatgcg ctcgacctcg aagtggacgc
    14221 cctccttcgt cgactcgatg tattcggcca cgacgccctc gaagtgctca cgatcaatgc
    14281 ggttgtccat ggaagagagc agcggcaaga cctcctcacg gacgcggagc ttgtacgtgc
    14341 gctcgtcctt cgcgttcgac ttgagggtct tcaagagaaa ttcgacgacg tggaccgact
    14401 tccccacgat cgccttgagg cgcttcgggt ccttacgaac gacgtccgca gggtcctcgc
    14461 cttccgggag ttcggcgact ttgacgtcca tgcccgcttc gagcatcgga cccgcggagc
    14521 gcttcaccgc ggtgattccg gcgcggtcgg catcgaggga gaggacgacg ttcttcgata
    14581 tgcgcgtgag gaggcccaca tggtgcggcg tgagcgccgt ccccgagacg gcgaccgcgt
    14641 tcacgtagcc cgcctggtgc gagaggacga ggtcgaactg tccctcgacg atgagcgcga
    14701 agttcatcga gcggatgccg tgcttcgcct tgtcgtagcc gtagagtatc tcggacttct
    14761 ggaagaggtc ggtctccgga gaattgacgt acttcggtac gccctcctcg gccttccgcg
    14821 tgcggcccga gaacgcgacg acgcggcccg aggggtccat gatggggaac atgacccggt
    14881 cgcggaagac gtcgtacggc tccttccctt ccgcgcgctt cgcgagccct gcggcgagga
    14941 gcgcctcgtc ggtgaagccc ttttccttca agtgcgcgcg caaggcgcgc cactcgtcct
    15001 tcgcgtagcc gatacggaag gactggatgg tcgcgcccgt tacgccgcgc tcctcgacgt
    15061 attttcgtat ctcgggcgct tcgcggagat tctcctcgaa gtagcgcgtc gcctcctcga
    15121 ggagcgcgta cgcggtctcg cgcgcatcac gcttcttcgg gtcttcgggg acgagctcga
    15181 cgtgcgcctt ctcggcgagt atcttgagcg cccccttgaa atcgacgccc tccatcttct
    15241 cgatgaaggt gaacatgtcg ccgccctgct gggaggagaa gcaatagtac atgccgcggt
    15301 cgggcgacac gtagaaggag ggcgtcttct cgttcgtgaa cggcgacttg cccttgtagc
    15361 tcttccccgc ccgcttcaat tcgacgtact gcccgatgat gtcgagaata ttgagccgct
    15421 cctttatctc ttcgaccggg gaccgcatga tggcaccatt atacgcgaag ggcacgacgg
    15481 cgggaaatgg cgcgaaaagg ccccggacgc gaagcgtccg gggccttttc tcgtactaaa
    15541 tcagtgctac tcctccacgg ggtcgttctc gaactgcttg atggcgtccc acttgcggga
    15601 gcccttgaag cccgtacccg ccgggataag tcggccgatg atgacgttct ccatgagacc
    15661 cgtgagcttg tcctcgctgc cgcgaacggc cgcgttgatg aggacgcgcg tcgtgttctg
    15721 gaaggacgcc gccgagagga acgactttct ggagagcgac gtctcggtga taccgaggat
    15781 gaggcgctcg cccttcgcgg gctccttgcc cgcggccttg agctcgtcgt tcacgcgcgc
    15841 gaggttccac tcctcgacga cgtcgccgac ggtgaaggag ctctcgcccg cctgggtgat
    15901 cttcacgcgg ctcatcatct gcttcacgat gagctcgatg tgctttcgcg cgatggtcac
    15961 accctggagt tcgtagatct tgttcgtctc gttgatgatg tactcctgcg tcgcctcctg
    16021 acccgcgtac ttgaagaggt ccgggaggtg cgccgagcca tcggtcatga gctggccctg
    16081 cttcacgctg tcgcccacgg agaccgtgat gacgcgacgg aagtggacgg cgtactcgat
    16141 gttgtcgttc ttcttcgtcg cgtacttcga gccgacggcc ggagcgacgg tgatgacctt
    16201 ctccttgccc tcggtgcgga tgttcgtgac gacgccgtcc gtgcggctga tgaccgccgg
    16261 gatcttcggt gcgcgcttct cgaacacctc ctcgacgcgc gggagaccct gcgtcacgtc
    16321 gccgccgacc gatgcggcgc ctccggcgtg cttcgtgttc atcgtgagct gggttcccgg
    16381 ctcaccgatc gcctgtgcgg cgacggtacc gaccgcctcg ccgatgtcga cgagcttatt
    16441 cgtcgtgagg tcgatgccgt agcactgctg acagacgccc tggagcgtct tacaggtcat
    16501 cgggctccgt acctcgaccg actcgatgtc ggacttctcg atcgcgatcg agtcgaggcg
    16561 cgagagaagg tggcccttct tgaacatgac cgtgccgtcc ttcgtcaccg cgtcagccgc
    16621 gaggatgcgg ccgcggatag ccttcgagaa ggcgatctcg atgcccgagg acgagatgcg
    16681 gccgatcgtg acgccctcct tcgtcttaca gtcgtcctcc gtgacgatgg cgtcctgcgc
    16741 gacgacgaag aggcgtcgcg tgaggtaacc ggccttcgcg gtctggagcg ccgtatcggc
    16801 gagaccctta cgcgagccgt gcgtcgtgat gaagtactcg atcgggctca taccctcctt
    16861 catcgaggag aggatgggga attcgagggt ctcgccgcgc gtgttgatga tgagaccctt
    16921 catgccggcc atctgcgcga tctggcccat ggagcctcgc gcgccggact tccacatatc
    16981 gaagacggag ccgttctcgt cgagagtcgc cgggagcgcc tgctcgatct cggtcttcgc
    17041 cttgtgccat atctcgatga tcatgcggcg gcgttcctcg tgggagatga ggccgtcgtt
    17101 gaagaatccg aagaccttcg cctcctcctc gcgcgccttc gcgatgatgg actgcttcac
    17161 ctcggggaca acgacgtcgt cgatgcccca ggtcgtaccc gacttcgtcg cgtactcgaa
    17221 gccgaaccgc ttcatgcggt cgacgatgcg cgggaccgcg tcgatgccct cttcgtcgat
    17281 gaggtcgacg atgatgccga agagcgttcg ctgggtgatg acgtcgttta tgaacggatg
    17341 ctccgacgga aggaccgagt tgaagagaag ccggccgacg gtcgtctcga agaccttacc
    17401 ctcgaactgc ttgtacttcg gcgtgtcggt cgcgaggacc tttaccttcg cacggatgtc
    17461 gagaacaccg aagtcgtacg cgagaatggc cgcgttcggt gaggagaaga acttgccctc
    17521 tcccttcgcg ccctccatga ccttcgtcat ccagaacgcg ccgagcgcca tgtcgaggag
    17581 cttctcggag acgaccgggt cggccgaacc gggcttcagg atgttcttgt tcgcggagat
    17641 gatctcgcgc gcctcgaact gcgcctcgtc ggagagcggt acgtggaccg ccatctggtc
    17701 gccgtcgaag tcggcgttga acgcgcgaca gacgaggggg tgaacctgga tggcgttacc
    17761 ctcgatgagg accgggcgga acgcctggat accctggcgg tgaagcgtcg gtgcgcggtt
    17821 gagaagtacg tgtcgatcgc gaatgacgtc ctcgaggatc gcccacacct ccggcacgcc
    17881 gtcctcaatg agacgacctg cgccgcggat gttgtacgcg agctcctgct tcaagagctc
    17941 ggagatgacg aacggacgga agagctcgag cgccatgtgc ttcgggagac cgcactggtc
    18001 gagtgcgaga tccggtccga tgacgatgac cgagcggccg gagtagtcga cgcgcttacc
    18061 gaggaggttc tgacggaagt agccctgctt cgacttgagg tagtctgcga gggacttaag
    18121 cggccttcgc tgtgcctggc tcatcgcgga gtacgcggtg ttgccgtggc gaatcgagtt
    18181 atcgatgagg gcgtcgacgg cctcctggag aatacgcttc tcgtttcgga gaatgacgtc
    18241 cggcgccatg atgtccatga gcttcttgag gcgattgttt cggttgatga cgcggcggta
    18301 gaggtcgttc aagtcactcg acgcgtgacg accgccctcg agggcgacca tcgggcgaag
    18361 cgccggcggg atgaccggga tgcgcgtgag gaacatccac tcgggtcgaa cgcccgcgtg
    18421 gatcatgcct ttgatgaggg agagacgctt gtcgagcttc gcgcgctccg ccgctccggc
    18481 ggtctcgcgc tccttctcga ggcgagcctg gagctcgacg aggtcgagct tcttgaagat
    18541 atcgtagatc gcctccgcgc cgatgcgcgc ctcgaagagg gtcgagtact tcatcgagta
    18601 cttgtggtag tgcatctcgt cgaggatggt gccctccgtg atgctctcga tctccgactt
    18661 gatgtcgtcc atgcggttct tcaattcctc gcgggcctgt tccgtttgga gctccttgta
    18721 cttcgtcttg aactcggtat cgagctcctt gaggatacgc tggcgctcgt ccgaagagac
    18781 cttcgtgacg atgtagcccg cgaagtagat gaccttctcg atcgacgccg atgacgtacc
    18841 gagtatgagc gcgatacgag acgggacgcc gcggaggaac caaatgtgcg agacggggac
    18901 gcagagctcg atatggccca tgcgctcgcg gcgcacgatg gaacgggtga cctccacccc
    18961 gcacttctcg cagacgatgc ccttgtaccg aatgcctcgg tacttcttgc aggcgcattc
    19021 gtagtcttca accggtccga agatgcgctc gtcgaagagc ccgtgcttct ccggacgctg
    19081 ggttcggtag ttgatggtct cgggcttcgt gacctctccc ttcgaccact cgaggatgcg
    19141 atcgggcgac gcgagcttga gcgcgacctc ggtgaactgc tgattggttt tcataatgat
    19201 gtgaagacta cgttgctaaa tcctgtcctc ctctcgacga aggtcgatgt cgagcgcgag
    19261 gccgcggagc tgattaacga gaacgttgaa ggctgccggc gtgtgcgggt gactgatctt
    19321 ctcgccgcgc acgatggcgt cgaacgccgc cgagcgaccg acgatgtcgt cggattttat
    19381 ggtgagcatc tcgcggagcg tgtacgcggc gccgtagccc aggagcgccc agacctccat
    19441 ctctccgaat cgctggcctc cgacctgtgc cttaccgccc aagggctgct gcgtgatcaa
    19501 ggagtacgga ccgatagagc gcatgtggat cttgtcctcg accatgtggt ggagtttcaa
    19561 gatgtacatg taaccgacgg agatgcgctg tgcgaacatg tctcccgtgc ggccgtcctt
    19621 gagccagagc ttgccgtcct ccggaagacc ggcggcctta agctcctcct tcacctcctt
    19681 ctcgcccgcg ccggcgaacg gcggaacgat agcctggtag ccgagcgtgt tcgcggccat
    19741 accgaggtgg agttcgagga tctgtccgag gttcatacgc gacggaacgc ccaagggggt
    19801 gagaatgacg tcgacgggac gtccgtccgc ggtgtacggc atgtcctcct cggggaggat
    19861 gcgggagatg actcccttgt tgccgtgacg gcccgcgagc ttgtctccga ccgagacgtt
    19921 acggacctgc gcgacggtga tgtggatgcg cttgatgacg ccggactcga gctggtcgcc
    19981 cgcctcgcgg ctgaatatct tcacgccgat gacgcggccg cgcttccccg cttcgaggcg
    20041 aagcgacgtg tccttcacgt ccttcgcctt ctcgccgaag atcgagcgga ggagacgctc
    20101 ctcgggcgtg agctgtgtct cgcccttcgg ggtcacctta ccgacgagaa tgtcgccggg
    20161 gcgtacctcc gcgccgatgc ggatgatccc ctcctcgtcg aggttcctaa gcttgagctc
    20221 ggagacgttc ggaatgtccg gggtcgtgac ctcggggccg agcttcgtgt cgcggaccga
    20281 gacctcgagc tcgtcgaggt ggatggtcgc gaacttgctg tccttgacga cgcgctcgga
    20341 gatgatgatg gcgtcctcgt agttcgcgcc gctccagctc atgaacgcga cgagcatgtt
    20401 ctgaccgagc gccatctggc cgttctcggt cgacgacgtg ttcgcgagaa tgtcgccgcg
    20461 cttcaccttc tgccctaccg agaccgctgc gcggtggtag aacgcggaga agtcgttcgt
    20521 tcgctggaac gtcgtgagcg cgtactcctt cttctttccg tccttattct tcaaaacgat
    20581 gtgacgagcg tctacttcga cgatctcacc cgcctcctcg gcgacgatca tgcggccggt
    20641 atcgcgcgca gcgagctcct cgacgccggt cgcgacgaac ggagcctcct gtacgatgca
    20701 cggagtagct tgcttctgca tgttcgaacc catgagcgcg cggttcgcgt catcgtggtt
    20761 gatgaacggg atcatcgagg tcgcgacgga gaacgcctga ttcgtcgcga tgtcgatgta
    20821 gtcgacctcc ttccgtgaga ccgagtgcgg ctcgccgtgg tagcgaacct cgagcgtctc
    20881 gggcttgatc ttgccgttat cgtcgacctc caccgcggcg tgcgcgatgt tgtacttctc
    20941 ctcctcgtgc gcattgaggt acacgatctc gtcggtgacg acgcccttct ccaccttcgc
    21001 gtacggcgtc tcgatgatgc cgaagcggtt cacccgcgcg tacatcgaga ggcgaaggat
    21061 gagaccgatg ttcggtcctt ccggcgtgtg aattgggcaa aggcgaccat agtgactggg
    21121 gtgcacgtcg cgcacctcga agctcgcgcg ctcgcgcgta aggccgccgg ggccgagggc
    21181 cgagagcgta cgaagtccct caagttctgc gagcatgttc tgctgctgca tgaactgcga
    21241 gagctggttc gtcgtgaaga actccttgat agcggcctga agcggacgcg gattgacgag
    21301 ctggatcggg agcgacgtct ccgcctcgat cgtcgacatg cggtcctgga tgttgcgctt
    21361 cacgcgcgtc ataccgacgc ggatacgctg ctggagcatc tcgccgaagt agcgaacgcg
    21421 gcggaagccg aggtggtcta tgtcgtccgc gatcgcgcca ggcgtgtgat tcgactcgat
    21481 gatgttcttc aagatagtga cgaggtcctc ggtcgtgacc gtgcgctgtg cgtcggcctt
    21541 cgcgtcggtc ttgaggccga agcgctcgtt gaagtggtgt cggccgatct cggagaggtc
    21601 gtagcgcaag gggctgaaga tggagtcgac gaactccttc gcgttctcga cggtcgcgag
    21661 atcgccgtcg cggagtcgct tgtagatctc gatgtacgag tcatcgagcg tcttcgcgac
    21721 gtccttcgcg atggtgcgct tcatgtactc gtcggcgtgc tcgacaccct tgaagagctt
    21781 caccatgtcc gcgtccttcg tgacgccgaa cacgcggaag agggacgaaa tggggaactt
    21841 tcgcttgcgg tcgatcttca cgtagatgac gccatcggcc tccgactcga tctcgaccca
    21901 tgcgccgcga gccgggatga tcttcgcacc gaagtacgtg cggcccttcg attccgtcgt
    21961 cgtgaagaag atgccgtacg aacgcgcgag ctgcggcacg atgacgcgct cgacgccgtt
    22021 tatgatgaac gtaccgttct cggtcatgac cggaaggtcg gcgaggaaga tctcctgctc
    22081 cttctcgctt ccgaaggtct tgttcttcaa gaccacctgc gcgcgaagcg gcgcctcgta
    22141 cgtgagcttg ttctcgtgcg cggcctcagg cgtcgtcttc ggactgccga gctcgtactt
    22201 cttgaaaatg aggtcgaact tcttctccga gtagtcgctt atcggactga actccttgaa
    22261 gacctccttg aggccgacct cgatgaagtt cttgtacgac tcacgctgcg gctcgacgag
    22321 atcaggaaga tctacgcgcg cgtcgcggaa gcgctgaaag tgcttcttcg gaaggaccgc
    22381 gccctcgttc tccttcgcct tcggtgcatt ggacatagta atgaatactc gttacgccgt
    22441 taataacggt gagcgaagcg atgaacgaaa aaacccgcac ccgaggcgcg tttcgtccat
    22501 acacttgtta ctcagcctgt actgtgtaca tcacgtgacg tgattatgtg tgaggcactc
    22561 gcctcgttgt ttcggtgtcc cccgacacca cccacgtccc ccgctaccgt ctgtatgaaa
    22621 atcgtgcaac cagaacgcac tccgccgatt tcgtatgcgc gagatattaa cagatacgac
    22681 gcttgacgtc aagcgttctc tccccttagg cacccgttga cgcgagcgat ctcgggtata
    22741 tagtgagaag acctcagaaa cgtgcgacgg ccgcctgccg cgacctccgc ccgagtaggc
    22801 cgctcatcgc ggaatacgca ggactcgtac gtttctgggg tcatcgccgc tacgtcggcg
    22861 attttttatg cctgacttcg cttccacgct tctatctcgg cgtgattgcc cttcgtaagg
    22921 ataggcggaa ccttgtattt cttgcctttg tggatgagaa cctcggggcg cgtgtacatg
    22981 acgcccgagg aaatccgctc gtcctcgagg gactcgaacg tgccgaggac gcccgggata
    23041 tggcgcgcg
//
LOCUS       DASTSP010000034         5960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_967557, whole genome shotgun sequence.
ACCESSION   DASTSP010000034 DASTSP010000000
VERSION     DASTSP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967557"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..759)
                     /locus_tag="VFS75_01455"
     CDS             complement(7..759)
                     /locus_tag="VFS75_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU4677366.1"
                     /translation="MDVEQSQTNTASGFSVRSEVYSGPLDLLIDLIEKRKLLVNDISL
                     ASVTDDYMAYVAQFERNPLREMADFIVLASTLLLLKSKSLLPVLELTDAEEETIESLE
                     ERLRYYQIFRNAAKSLAGRFGRTVAFGRPFVPDRRPLFVTDSYTTVDALRSAMSDVVR
                     NLPVKREKPKVQVKTVVSLETMIERLRDRIEKQFKLRFRDFAGSGQRTTVIVGFLAVL
                     EMVKQGVLIVRQAARFDDIELEREARTTPRYQ"
     gene            complement(816..2249)
                     /locus_tag="VFS75_01460"
     CDS             complement(816..2249)
                     /locus_tag="VFS75_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEU4677367.1"
                     /translation="MQIRTTDKKLKALPKDYVRVDFVEKGAETLMREGKATTLTVALG
                     KEKDLSPRTFRTLVRSVVQAAHRHKIMKLAFSFELSNYPKLAQHGEAWALSQIAENLL
                     LADYAFTNYKSKPGRERLTEVLIAGKFTKEGLAGLRRGVTVGKAANACRDIANTPGGD
                     MTPKTLAERAKEELTGTKATVTVLDEKAIRELGMGGVLGVGQGAADKPRFIVIEYWGL
                     KKPMMTGAKGAMKFKDDERPIVFVGKGVTFDTGGLQVKPGMAMYEMHMDMSGGAAVIA
                     AIGAIARLGLQKNVVGLVPAVENAVSDRAIRPGDVLRLMSGKTVDVLHTDAEGRIILA
                     DALTYAKRYEPKLLVDVATLTGAAMVAVGQHAHVIMTKDRALEDRFRELGELSGDYTF
                     PLPLWDEYLQYTKGVHGDIANVPSTGDSRAGGAINGGTFLSHFAEKMPWVHIDMAARM
                     TSVASDKLARGATGEPTRLLVKIAEEF"
     gene            complement(2218..3591)
                     /gene="aspS"
                     /locus_tag="VFS75_01465"
     CDS             complement(2218..3591)
                     /gene="aspS"
                     /locus_tag="VFS75_01465"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEU4677368.1"
                     /translation="MERTLIRELRNEIGKTVTIKGWVDVARNQGKVAFFDFRDRTGTV
                     QGVVFGKPDLAPVAAELKQEWVVAVTGVVNKRPEKMVNAKAENGDIELEITGIEVLAK
                     AAPLPFDMSAEGYNLDLTTELDHRALTLRHPKNRAIFKVQAVIIDAFREYLKSQDFFE
                     FQAPAITPATAEGGAEVFQVNYFDKKAYLTQSPQLYKQIVMTAFERVFSVNKVFRAEP
                     SATTRHITEIVSLDAEMGFIDSWTDVRVMSENTVRYILGEVAKRCAKELELLDATLPV
                     MIEKTPTYSLTEAQELIFEKTGRDCRGEKDLSPEDERAICEIVKADTGSDFVYVYGYP
                     TRQKPFYVYPNPEKPEFNEGMDLLCRGIEWLSGGRRINDYEQLMDHVEKWNMNPDAIA
                     MFLEAFRYGVPPEGGFAFGAERMTMQILGLKNIREATLFPRDMHRIDTLLAKPDDHAD
                     TNDGQEA"
     gene            complement(3862..4809)
                     /gene="trpS"
                     /locus_tag="VFS75_01470"
     CDS             complement(3862..4809)
                     /gene="trpS"
                     /locus_tag="VFS75_01470"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU4677369.1"
                     /translation="MFGIGKKRLLTGIQPSGALHLGNYLGALRPFMDVYRDYESFIMV
                     ADLHALTTVRDPKALRGYVRDVVRDYLAAGVDPKKAVIFRQSDVAAHAELAWIFECLV
                     TVKFLELAHAYKDKVAKGLEANAGLFNYPMLMAADILLYDADVVPVGEDQRQHVEYAR
                     EAAAKFNHAYGDVFKAPKEMILKGVGVVPGTDGQKMSKSYKNTIPLFGTKEELVKAVM
                     GIVTDSSGERPENVYAIHKLVRSEAELTPLYEENKGKYKNLKQALVADLEALIAPMRE
                     RRNAITDREVEEVLEDGAEAAGTVAAKKMAAVKDAVGLS"
     gene            complement(4913..5539)
                     /locus_tag="VFS75_01475"
     CDS             complement(4913..5539)
                     /locus_tag="VFS75_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677370.1"
                     /translation="MKILRMSAAFMVLFGIMATASAQWAVTDPSEPSYSAELQLQYQA
                     TRNASEQRSLFAKSLYVEKSLGGSVSVTFIGYHDEEFWSMYPGLAWSHGNVEVGLGVG
                     SAHYDGRSHTTLNPWVWYGNDELGIEAQVYVERYLSEHDDPWYVKAYVRKRVGDLMFG
                     VYEDSIMGAGPLAGVYLGKHLRLWAYVPVVGQPSTGRMRGAITLTYEF"
BASE COUNT         1079 a         1804 c         1806 g         1271 t
ORIGIN      
        1 cgcgcatcat tggtatcgtg gcgtcgttcg cgcctcgcgc tcgagctcga tatcgtcgaa
       61 ccgcgccgcc tgacggacga tgaggacacc ctgcttcacc atctcgagga ccgcgagaaa
      121 accgacgatg accgtcgtcc tttgcccgga gccggcgaag tcgcggaagc gaagcttgaa
      181 ctgcttttcg atgcggtcgc ggagccgttc gatcatcgtc tcgagcgata cgaccgtctt
      241 cacctggacc ttcggcttct cgcgcttcac ggggaggttt cgaacgacgt ccgacatcgc
      301 gctccggagc gcgtcgacgg tcgtatagga atccgtcacg aagagaggcc gacggtcagg
      361 tacgaacggt ctgccgaacg cgacggttct accgaagcgg cccgcgaggg acttcgccgc
      421 gtttcggaat atctgatagt accgaagacg ctcctcgagc gactcgatgg tctcctcttc
      481 cgcgtcggtg agctcgagta cgggaaggag cgatttcgac ttgagaagga ggagcgtcga
      541 ggcgagcact atgaaatccg ccatctcgcg aaggggattt cgctcgaact gcgcgacgta
      601 cgccatgtag tcgtcggtga ccgacgcgag cgaaatgtcg ttgacgagga gcttacgctt
      661 ctcgatgagg tcgatgagga ggtctagcgg gcccgaatag acctcgcttc gtacgctaaa
      721 accgctcgcc gtattggttt gagattgctc tacgtccacg agtccatttt accatggaac
      781 gagcgagcgg tcgttctcgt atccaccaaa aaaagctaga attcttcggc tattttgacg
      841 aggaggcgcg tcggctctcc cgtcgcaccc ctcgcgagct tatccgacgc gaccgatgtc
      901 atgcgggcgg ccatgtcgat gtggacccac ggcatcttct cggcgaaatg cgagaggaac
      961 gtgccgccgt ttatcgcgcc gcccgcgcgc gaatcgccag tggacggtac gttcgcgatg
     1021 tcgccgtgga cgcccttcgt gtattggagg tactcgtccc agagagggag cgggaacgtg
     1081 tagtcgccgc ttaactcacc gagctcgcgg aatctatcct cgagggcacg gtccttcgtc
     1141 atgatgacat gggcgtgttg accgaccgcg accatagccg cgccggtgag ggtcgcgacg
     1201 tcgacgagga gcttcggctc gtagcgcttc gcgtacgtga gcgcgtcggc gaggatgatg
     1261 cgtccctcgg cgtcggtatg gagaacgtcg acggtcttcc ccgacatcag gcgaagcacg
     1321 tcgcccgggc ggatggcgcg gtcagagacc gcgttctcga ccgcgggaac gagaccgacg
     1381 acgttctttt ggaggccgag gcgcgctatc gcgcctatcg ccgcgatgac cgcggcgccg
     1441 cccgacatgt ccatgtgcat ttcgtacatc gccatgcccg gtttcacctg cagtcctccc
     1501 gtgtcgaacg tgacgccctt cccgacgaag actatcggtc gttcatcatc cttgaatttc
     1561 atggcgccct tcgctcccgt catcatgggc ttcttaagtc cccagtactc gatgacgatg
     1621 aaacgcggct tatccgcggc tccttgccca acccccaaga cgccgcccat accgagctcg
     1681 cgtatcgcct tctcgtcgag aaccgtgacg gtggcctttg taccggtgag ttcctctttc
     1741 gcgcgctcgg cgagcgtctt tggcgtcatg tcgccgccag gcgtgttcgc gatgtcgcgg
     1801 caggcgttcg ccgccttgcc gacggtgacg ccgcgtctaa ggccggcgag cccctccttc
     1861 gtgaacttcc ccgcgatgag gacctccgta agccgttcac ggccgggttt gctcttgtag
     1921 ttcgtgaacg catagtccgc gaggaggagg ttctcggcga tctgtgagag cgcccacgcc
     1981 tctccgtgtt gtgcgagctt cgggtagttc gagagctcga acgagaacgc gagtttcatg
     2041 atcttgtgtc ggtgcgccgc ctgcacgacg gaacgcacga gcgttcgaaa cgtgcgaggc
     2101 gagagatcct tttccttgcc gagcgctacc gtgagcgtcg tcgccttccc ctccctcatg
     2161 agcgtctccg cgcccttctc gacgaagtcg acgcggacgt agtccttggg gagtgcctta
     2221 agcttcttgt ccgtcgttcg tatctgcatg gtcgtctggc ttagcgagca atgtgtcgat
     2281 tcggtgcatg tcgcgcggga aaagggtcgc ctctcggatg ttcttcagtc cgagtatctg
     2341 catggtcatg cgctccgcgc cgaacgcgaa gccgccttct ggaggtacac cgtaacggaa
     2401 cgcctcgagg aacatggcga tcgcatcggg gttcatgttc cacttctcta catggtccat
     2461 gagttgttcg tagtcgttga tgcgccgacc tcccgaaagc cactcgatgc cgcggcagag
     2521 gaggtccatg ccctcgttga actcgggctt ctcggggttc gggtacacgt agaacggctt
     2581 ctggcgcgtc ggatagccgt atacgtacac gaaatccgag cctgtgtccg ccttcacgat
     2641 ctcacagatg gcgcgctcgt cctcggggct caaatccttc tctccccggc agtcgcggcc
     2701 ggtcttctcg aagatgagct cctgcgcctc ggtgagggag tacgtcggcg tcttctctat
     2761 catgacgggc aaggtcgcgt cgaggagttc gagttccttc gcacagcgct tcgccacttc
     2821 gccaagaatg taccgcacgg tgttctccga cataacgcgg acgtcggtcc acgagtcgat
     2881 gaatcccatc tccgcgtcga gggagacgat ctcggtgatg tgccgcgtcg tcgccgaggg
     2941 ttcggcgcgg aagaccttgt tgaccgagaa gacgcgctcg aacgcggtca tgacgatctg
     3001 tttataaagc tgtggcgact gcgtgaggta cgccttcttg tcgaagtagt tcacttgaaa
     3061 cacctccgcg ccgccttcgg cggtcgccgg cgtgatcgcg ggtgcctgga actcgaagaa
     3121 atcctggctt ttgagatact ctcggaacgc gtcgatgatg accgcctgca ccttgaagat
     3181 ggcgcggttc ttcgggtggc ggagcgtgag cgcgcgatgg tcgagttcgg tcgtgaggtc
     3241 gaggttgtat ccctcggcgg acatatcgaa cggaagcggc gccgccttcg cgagcacctc
     3301 gatgccggtt atctcgagct cgatgtcgcc gttctcggcc tttgcgttca ccatcttctc
     3361 gggtcgcttg ttgacgacgc cggtcaccgc gacgacccac tcctgtttaa gttccgcggc
     3421 gacgggcgcg aggtccggct ttccgaaaac gacgccctgc acggtgcccg tgcggtcgcg
     3481 gaaatcgaag aaggcgacct tgccttggtt ccgggcgacg tcgacccaac ccttaatggt
     3541 caccgtcttc cctatctcgt ttcggagctc tcgaatgagc gttctttcca tgtgaatcgt
     3601 tgccgtaggg acgaaaaacc gcccccacgg acatattacg aataatacgt ccgtaggggc
     3661 ggttttggcc gcggtgccac cctacttccc cggcggtatg ccgcgcgggg ccctcgttag
     3721 ttatcacctt acaggtgctg tacaccttgt cgcatagtta tgctcaaggt cttgcgaaac
     3781 gggtcccgtc ccgtttctca cctcggcagg tgtcagctgc gtcaacggtt cttcgaatcg
     3841 taccgccgtc gtttcgccgc gtcaagaaag gccgaccgcg tccttcacgg ccgccatctt
     3901 cttcgcggcc accgtccctg ccgcctcggc gccgtcctct aggacttcct cgacctccct
     3961 gtccgtaatg gcgttccgtc gctcgcgcat cggcgcgatg agcgcttcga ggtcggcgac
     4021 gagcgcctgc ttaaggttct tgtacttacc cttgttctcc tcatagagcg gcgtgagctc
     4081 cgcttcagaa cggacgagct tgtgtatcgc atagacgttc tccggtcgct cgcccgaaga
     4141 gtcggtgacg atgcccatga ccgccttcac gagctcttcc ttcgtgccga agagcgggat
     4201 ggtgttcttg tagctcttcg acatcttctg gccatcggtc ccgggaacga cgccgacacc
     4261 tttcaggatc atctccttcg gcgctttgaa gacgtcgcca tacgcgtgat tgaacttcgc
     4321 cgccgcctcg cgcgcgtact caacgtgctg gcgctggtct tcgcccaccg gaacgacgtc
     4381 cgcgtcgtag aggagaatat ccgcggccat gagcatcggg tagttgaaga gaccagcgtt
     4441 cgcctcgagt ccctttgcga ccttatcttt gtacgcgtgc gcgagctcga ggaacttcac
     4501 ggtgacgagg cactcgaata tccatgcgag ctcggcatgc gcggcgacgt ccgactggcg
     4561 gaagatgacc gccttcttcg ggtcgacgcc cgcggcgagg tagtcgcgga cgacgtcacg
     4621 aacgtagccg cggagcgcct tcgggtcgcg gaccgtcgtg agcgcgtgga ggtcggcgac
     4681 catgatgaaa ctctcgtagt cgcggtacac gtccataaac ggccggagcg cgccgaggta
     4741 atttccgagg tggagcgctc ccgacggctg aatgccggtg agaagtcgct ttttgccgat
     4801 tccgaacata cgaaaatagt agcagaatcg tgaggctgca gaaaggcaca ttgtgggaac
     4861 gcatagaaaa agggcatggt cggtgagacc atgcccttaa ggtgcttcag tgctagaact
     4921 cgtacgtgag cgtgatggct ccgcgcatgc gaccggtcga cggctgtccg acgaccggta
     4981 cgtacgccca gaggcggagg tgcttgccga gatagactcc ggcgagcggc cccgcgccca
     5041 tgatggagtc ctcgtagacg ccgaacatga ggtcgccgac gcgcttacgc acgtaggcct
     5101 tcacgtacca ggggtcgtcg tgctcgctga ggtatcgctc gacgtacacc tgcgcctcga
     5161 tgccgagctc gtcgttcccg taccagaccc acggattgag ggtcgtgtgc gagcggccgt
     5221 cgtagtgagc gcttcccacg ccgaggccga cctcgacgtt accgtgcgac catgcgaggc
     5281 ccgggtacat cgaccagaac tcctcgtcgt ggtacccgat gaacgtgacg gacacgctcc
     5341 caccgagcga cttctcgacg tagagactct tcgcgaagag cgagcgctgt tcgctcgcgt
     5401 tccgcgtcgc ctggtactgg agttggagtt ccgccgagta gctcggctca gacgggtccg
     5461 tgaccgccca ctgtgcggac gcggtcgcca tgatcccgaa aaggaccatg aacgccgcgc
     5521 tcatgcgaag gatcttcatg tgccctcctt tgggcggttg tagaaactgt tcgcattata
     5581 ctacactaaa aaagtatcgt caaatcattg taattcatta aaaaaatggt atgttaaaaa
     5641 cgtcgcaaat cctcaaagga aggaggtgag ataaatgaag agcccgaagc agttctcgtg
     5701 gaaagtcacc acggtggccc gggatgagcc caagaagaag ggcaatttcc agagccagtg
     5761 ccgcagcggt ggcggtggca acggccggtg cggtggcaag tgcaagcagt aaagcagtga
     5821 gagggtgtgg cctcggccac accctttaat catttctaga aaaactttga tcgggaaagt
     5881 attttttgtg gtctctcctt acaaagatcc ctccacgaaa tgccaggctc ctcccttttc
     5941 aagttcttta ttatccagta
//
LOCUS       DASTSP010000035         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_302907, whole genome shotgun sequence.
ACCESSION   DASTSP010000035 DASTSP010000000
VERSION     DASTSP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302907"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..571
                     /locus_tag="VFS75_01480"
     CDS             74..571
                     /locus_tag="VFS75_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU4677371.1"
                     /translation="MRNLSLFALLLILVGFGYLLWHKNAEAPVAQTEDRPDTSAPFET
                     TNPAAGLPAITDVSGTDAGMNPEADPTLPAHTKVFNVDASNFSFSVKEMRVNVGDTVI
                     VNFANKDGFHDWVIDEFNARSPRIGAGQSAQITFVADKKGSFEYYCSVGSHRAQGMWG
                     TLVVE"
     gene            642..1274
                     /locus_tag="VFS75_01485"
     CDS             642..1274
                     /locus_tag="VFS75_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677372.1"
                     /translation="MSFELPGRAERHGSDLAIRFKELLTERGPRVFKELERTLEEHGF
                     ALASAEDVALLFSENSLLCRNENFTRVMDLLIDGTPIAVRNREYQANMCIVAGGEGFR
                     VAMSEGFSGEDVGGVVRTVVTFRGRHLSSRDTIPHDAELWALKPKTAQVSIAARGEIP
                     LDDIEMVSFRFPTEYFPEELLTDDERERLNEAGIRFIVRHYIPERKTATH"
     gene            complement(1275..1556)
                     /locus_tag="VFS75_01490"
     CDS             complement(1275..1556)
                     /locus_tag="VFS75_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020039556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU4677373.1"
                     /translation="MHGSDFLNRQRKRLHSRKASKLQILKDGGEQSLSHSEKDIIYIE
                     YALRRIDENQYGLCSNCGTPIRRERLEAIPEALNCIECQSTTETKSGHA"
     gene            complement(1619..2545)
                     /gene="xerA"
                     /locus_tag="VFS75_01495"
     CDS             complement(1619..2545)
                     /gene="xerA"
                     /locus_tag="VFS75_01495"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEU4677374.1"
                     /translation="MTLRDLKSRFLEYLEIEKGRSIHTVSNYDRYLTRFLEYAKTDDP
                     AALTEGAVREFRLWLNRQPGVKNGRNTDTMKRKTQNYYLIALRAFLKYLRKQGVASLN
                     PERIELAKVPDRSLDLISPQELDRLMKAPDVSTLRGLRDRAILELLFSTGLRVSELCN
                     LSQEDIDLSRDEFSVRGKGDKVRVVFLSDTAREAVRAYLKERKDFDDAMFVQYGKTMK
                     KDEVDNKDLRLTPRSVQRIIKRYATIAGITRKVTPHVIRHSFATDLLSNGADLRSVQA
                     LLGHANIATTQVYTHVTDKHLRDIHKKYHSRK"
     gene            3091..3357
                     /gene="rpsO"
                     /locus_tag="VFS75_01500"
     CDS             3091..3357
                     /gene="rpsO"
                     /locus_tag="VFS75_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU4677375.1"
                     /translation="MLTKRKKENAMKEVRRHDSDTGSPEAQVAILTKQIDELSKHLKK
                     NAKDFHSRRGLLQMVADRRKHMKYLERKDEKAYKSLVKKLDLKK"
     gene            3499..>3797
                     /locus_tag="VFS75_01505"
     CDS             3499..>3797
                     /locus_tag="VFS75_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU4677376.1"
                     /translation="MSVIKHADQRVAIFIDTQNLYHSAKNIHHAKVNFGAVVKAALGG
                     RKLIRAMAYVVTTESGEEQSFFEALEKTGIEVKTKDLQVFFGGAKKADWDVGLAV"
BASE COUNT          813 a         1078 c         1103 g          803 t
ORIGIN      
        1 ttcttcatcc acctctaggc tctccttcga actcgatgct atacttcggt tctaccattt
       61 aatgcaatcg tccatgcgga atctttcact cttcgcgctt ctccttattc tcgtcggctt
      121 cggctacctc ctctggcata agaacgccga ggcaccggtc gcccaaacgg aggatcgtcc
      181 cgacacgagc gcaccgttcg agaccacgaa tcccgctgct gggcttccgg cgatcaccga
      241 tgtctccggc accgatgcgg ggatgaatcc cgaggccgac ccgaccttac cggcgcatac
      301 gaaggtcttc aacgtcgacg cgagcaactt ttcgttcagc gtgaaggaga tgcgcgtgaa
      361 cgtgggtgac acggtcatcg tcaatttcgc gaacaaggac ggcttccatg actgggtcat
      421 cgacgaattc aacgcgcgct caccgagaat cggtgccgga cagtcggcgc agatcacgtt
      481 cgtcgcggac aagaagggaa gcttcgagta ctactgctcg gtcggttcgc accgtgcaca
      541 gggcatgtgg ggaacgctcg tcgtcgagta gaacgctccg acacgcttga aaacccgggt
      601 gtttcgttta catttctctc ctcgtggggg acaatatctt catgtcattc gagctccccg
      661 gtagagcgga acgtcacgga agcgatctcg cgattcgttt caaagaacta cttacggaac
      721 gcggtcctcg cgtcttcaag gagcttgagc gtactctcga agaacatggg ttcgcgctcg
      781 cgtcggcgga agatgtcgct ctattgtttt ccgagaactc gctcctctgc agaaacgaaa
      841 actttacgcg cgtcatggac ctcctcatcg acgggacgcc gatcgcggta cgaaacagag
      901 agtaccaggc caacatgtgc atcgtagccg ggggagaggg cttccgcgtc gcgatgagcg
      961 agggcttcag cggtgaggat gtcggtggcg tcgtccgcac cgtcgtcacg ttccgcggta
     1021 gacacctttc gtcgcgcgat accattccgc acgatgcgga attatgggcg ctcaaaccga
     1081 agactgcgca agtatccatc gcggcgcgcg gcgagatccc gctcgacgac atcgagatgg
     1141 tctcgtttcg attccccacg gagtactttc ctgaagaact cctcacggac gacgaacgcg
     1201 agcggctcaa cgaagcgggc atacggttca tcgtccgcca ctatattccg gaacggaaaa
     1261 cggccacgca ttgatcatgc gtggcctgac ttcgtctcgg tcgtgctttg acactcgatg
     1321 caattgagcg cctcgggaat cgcctcgagg cgctctctac gtatcggcgt accgcagttc
     1381 gagcagagac cgtactggtt ctcgtcgatg cgccgaagcg catactcgat gtagatgatg
     1441 tccttctcgg agtgcgagag cgattgttcg ccaccatcct tgaggatctg caacttcgac
     1501 gcttttcgag aatggaggcg ttttcgctga cgattcaaga aatcactgcc gtgcatcgac
     1561 cctcccaaag aacgttatgg ttggccgcta ttatgccatc ttgactcata ttagtcaact
     1621 acttccgcga gtggtacttc ttgtgaatgt cacggaggtg cttgtcggtc acgtgcgtgt
     1681 agacctgcgt cgtcgcgatg ttcgcgtgcc ctaaaagcgc ctggaccgag cgaaggtcgg
     1741 cgccgttcga gagcaggtcg gtcgcgaagg aatggcgaat gacgtgcggc gtcaccttcc
     1801 gcgtgatgcc cgcgatcgtc gcgtagcgct tgatgatgcg ctggaccgag cgcggcgtga
     1861 gccggaggtc cttgttgtcg acctcgtcct tcttcatcgt cttcccgtac tggacgaaca
     1921 tcgcgtcgtc gaagtccttt cgctccttga ggtacgcgcg caccgcctcg cgcgcggtat
     1981 cggagaggaa gacgacgcgc accttatcgc ccttgccgcg gaccgagaac tcgtctcgcg
     2041 agaggtcgat gtcctcctgt gaaaggttgc agagctcgga gacgcgaaga cccgtcgaga
     2101 agagaagctc gagaatggcg cggtcgcgga gaccgcggag cgtcgagaca tccggtgcct
     2161 tcatgaggcg atcgagctct tggggcgaga tgaggtcgag cgaccggtcc ggcaccttcg
     2221 cgagctcgat acgctcggga ttcaaggaag cgacaccctg cttacgcagg tacttgagaa
     2281 acgcgcgaag cgcgatgagg tagtagttct gcgtctttcg cttcatcgtg tcggtgtttc
     2341 gaccgttctt cacgcccggt tggcgattga gccagaggcg aaattcgcgg acggcgcctt
     2401 cggtgagcgc cgcggggtcg tctgtcttcg cgtactcgag gaaacgggtg aggtaccggt
     2461 cgtagttcga gaccgtatgg atgcttcgcc ccttttctat ctcaaggtat tcgaggaatc
     2521 tgcttttgag gtcacgaagc gtcatggaga tattatacac accatacgtc gcacaatatt
     2581 cggcgcgcga agatgaaaaa ggccgcgctc ccacgcgtgg cctcgtcgtg accgactatt
     2641 ttcgaaggag aggtcgcttc aaaacctcct cgcgcatgta gggaagcgag aggtggaaca
     2701 tttctgcgac cactgtagcg gcttccccca cggaacctga cgcctccgag ccgaaaatag
     2761 ccagtctccg acgccccgca tcgaagtccg cctcggcctg acgctcgagt tccgcacgga
     2821 gcccttcaag aaccagaaga agcgcatcgt accgtaattt cccgattctg cggcccaatt
     2881 cctcgagtgt atccgcacgt ggcggatcga tcggatggtc cacgtgatgt cctttctttt
     2941 aggggtctgg caacgagcat accaaaaaag cgttagggca ggggttgcaa aaattgtcaa
     3001 cgtgtggtaa agtagtacta ccttcgcagg ttaggatcta acggcgtccg agtgttctat
     3061 ctaagcactc gcgacgcgtt tttaaacatc atgcttacaa aacgaaagaa agagaacgcg
     3121 atgaaggaag ttcgacggca cgacagcgat acgggttcgc ctgaagcaca ggtcgcgatc
     3181 ctcacgaagc agatcgacga gctctcgaag caccttaaga agaacgcaaa ggattttcat
     3241 tcccgccgcg gtcttcttca gatggtcgcg gaccgtcgca agcacatgaa gtatcttgag
     3301 cgcaaggacg agaaggcgta caagagcctc gtgaagaagc tcgatctcaa gaagtaaggt
     3361 cagaggtgag gatccgcccg gaccgggcgg attttcattt tccgccgatg acgcatctgt
     3421 tatactagcg aaggaggggc ttttcggccc acttttacaa aacagtacta acgaacgagt
     3481 gatcgttcac gcatttttat gtcagtaatc aaacacgcag accagcgtgt cgccattttc
     3541 atcgacacgc agaacctcta ccatagcgcg aagaacatcc accacgcgaa ggtcaacttc
     3601 ggcgcggtcg tcaaagccgc cctcggcggc cggaagctca tccgcgcgat ggcgtacgtc
     3661 gtcacgacgg agagcggcga ggaacagtcg ttcttcgaag ctctcgagaa gacgggtatc
     3721 gaggtgaaga cgaaggacct ccaggtcttc ttcggcggcg cgaagaaggc ggactgggac
     3781 gtcggcctcg ccgtcga
//
LOCUS       DASTSP010000036         6150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_527474, whole genome shotgun sequence.
ACCESSION   DASTSP010000036 DASTSP010000000
VERSION     DASTSP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527474"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFS75_01510"
     CDS             <1..429
                     /locus_tag="VFS75_01510"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU4677377.1"
                     /translation="IGEENSVDSPNTAHVRAIIGDTFNASRAELPLQAGEYVLYKLWK
                     RGKRIDWPLRREARVPGLWHKLLRGVPRIHIGVTPKVGDIMEYVMEGEVGHLAYVEAV
                     FPDDTITVSEANFPDSGIYNERVLTREEWRELRPTFVSVL"
     gene            452..1135
                     /locus_tag="VFS75_01515"
     CDS             452..1135
                     /locus_tag="VFS75_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU4677378.1"
                     /translation="MQKAAFFDIDGTLFRSSLLIELVEECIAAGIFPSDTRGRYADEF
                     RAWQFREGTYEAYIDAVVDAFIENIRGVHYGAFVRVGEAVVERAGKKTYRYTRDLIAD
                     LKGKGYYLVAISQSPKTILDEFCAAYGFDKVYGRIYEIGPRDLFTGAITDVELIKDKS
                     LIVKRVFERHELDREGSIGVGDTEGDIPLLSSVDTPICFNPNMQLYQHAKEKDWPIVV
                     ERKDVVYTI"
     gene            1143..1943
                     /locus_tag="VFS75_01520"
     CDS             1143..1943
                     /locus_tag="VFS75_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU4677379.1"
                     /translation="MTKLAAFRRRVRGFYEREGRHALPWRTTRDPYRILVSEVMLQQT
                     QVERVIPKYEAFLKRFPTARSLAKARLGDVLRLWQGLGYNRRAKLLHDAAKVIASKRS
                     FPRTLEELRALPGVGTYTAAAVMTFAFDEPVPLIETNVRTVFIHHFFADEDGVDDDAL
                     MPLIERTLDRTRPREWYYALMDYGSHLKKTVGNASARSVHHVRQSAFKGSDREVRGAI
                     VRTLAAGPKQRETLHASLPFDLQHIDEQLERLIEEGMVVRRRAAYALP"
     gene            complement(2009..3988)
                     /locus_tag="VFS75_01525"
     CDS             complement(2009..3988)
                     /locus_tag="VFS75_01525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU4677380.1"
                     /translation="MEQQSNQFNSERRAANFSGRPSGRPSRRGRGGANRRGGRRSTFS
                     ADGARTPRDGQSPQGEKRRRPSQNRRRLERNRARTSPALTHRIAPVDAKGPRILPNTD
                     PDVVRIVPLGGVEEVGKNMTVVETADDILIFDAGFQFVSSESDAPGVNYILPNTQYLE
                     ENKHKIRGIVITHGHLDHIGGIPFLIERIGNPPIYTRYLTSLMILKRQEEFPHLPKVN
                     MNVVELDQRIKIGNTYIKPFPVTHSIPDSMGVSVETKHGNIVISGDLKLEHDGTEPSE
                     KEKRVWGGVGKDKNILFIGDSTNAEREGFSIPETRVHTNIEQIIRNVKGRLIVGTFAS
                     QFERMIRIIMICEELGKKIVLEGRSIKTNLEIAELAGLMKVQKGTIIPIQEIGNYPSD
                     RIVVLATGAQGEEFAALMRIATGRHKFLTLNERDTIMLSSSVIPGNELSVQKLKDNLY
                     RHGVHIIHYRASDVHSTGHGNSGELVWIRAQVNPQFVMPAYGYHSMLRCHAYANIEAG
                     FPKERVILADNGSVIDIKNGTELHVHKQKAPSEIVMVDGFTVGAKQEVVLRDRQSLAE
                     DGMFVIIATVNTKTGALRKSPDIISRGFVYLRENQQLLSEARVLVKKTIEKYTAKSHP
                     IDLDYVKDEITNVVSGFLLQKTNKTPMVIPVLIGI"
     gene            complement(4030..5724)
                     /locus_tag="VFS75_01530"
     CDS             complement(4030..5724)
                     /locus_tag="VFS75_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4677381.1"
                     /translation="MFLRLDRMKASDGFLLKGALLAAVAFLAVFLIRASVDHSVLIPA
                     TGGTFSEGIVGTPRFANPVLAVTRADRDLSALVYDGLMRLGEDGTLVPNIAKSVTVSD
                     DGLTYNVVLRDDVKFHDGQPLTAKDVAFTVGRIEDPLLSSPLRANFDGVSVEVLGDYE
                     LNFVIAQPYAPFIENLTFGILPEHIWKDAGSDEFPFSQYNSEPVGSGPYEVESIARNA
                     SGIPETYVLKANDAYHRGSPKIKTIELHFYPSEDKLIQAFKKGTVGSVAGVDPDHLAA
                     YQVNSATSHVERIPLPRTFAVFFDQNKSAALRDPAARKALDAAIDRSALIDAVLGGYG
                     RPLFGPVPEGFGTNASATPHADVVGVDAARDILKAGGWEMNDETHVWEKTIDKVKTPL
                     SFSLSTANGGTFEATAEFLKNAWEQLGAAVEVKQFEQTDLAQSVIRPRDYQALLFGMQ
                     LGRAFDFYSFWHSSQRNDPGLNIALYANLTTDSILADMRTSTDTASRNDAIDAFVAEL
                     DKEKPAVFLYSPELLYIFPNDVTGATFTGLAEPQERWSGVTDWYIKTDSVWPIFTK"
     gene            complement(5870..6100)
                     /gene="secG"
                     /locus_tag="VFS75_01535"
     CDS             complement(5870..6100)
                     /gene="secG"
                     /locus_tag="VFS75_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU4677382.1"
                     /translation="MEIVRTVLPYVQIILSILLVSAILLQQRGSSLGGAFGGDNFSAT
                     YHKRRGAELFLFRFSIATAILFVASTFLSFLI"
BASE COUNT         1147 a         1811 c         1992 g         1200 t
ORIGIN      
        1 ataggggagg agaactccgt cgatagtccg aacacggcgc acgtccgcgc catcattggc
       61 gatacgttca atgcgagccg tgcggagttg ccgctccagg cgggcgagta cgtcctctat
      121 aagctctgga agcgcgggaa gcgcatcgac tggccgctgc ggcgagaagc tcgcgtgccg
      181 ggcctctggc acaagctcct tcgcggcgtt ccgcgcatcc acatcggagt cacgccgaag
      241 gtcggcgaca tcatggagta cgtcatggag ggcgaggtcg ggcacctcgc gtacgtcgag
      301 gcggtattcc cggacgacac gatcaccgtc tccgaggcga acttccccga ctccggtatc
      361 tacaacgagc gcgtgctcac gagggaagag tggcgcgaac tacggccgac gttcgtgagc
      421 gtcctctagc gacctttggt acactatgac catgcagaaa gccgcattct tcgacatcga
      481 cggtacgctc ttccgttcga gcctcctcat cgagctcgtc gaggagtgca tcgcggccgg
      541 tatcttccca tccgacacgc gcggacgcta cgcagatgaa ttccgcgcgt ggcaattccg
      601 cgagggaacg tacgaggcct acatcgacgc tgtcgtcgac gcgttcatcg aaaacatacg
      661 aggcgtgcac tacggcgcgt tcgtgcgcgt cggtgaggcg gtcgtagagc gcgccgggaa
      721 gaagacgtac cgatacaccc gggacctcat cgcggacttg aagggaaagg gctactacct
      781 cgtcgccatc tcgcagtcgc cgaagacgat cctcgacgag ttctgcgccg cctacggctt
      841 cgacaaggta tatggccgta tctacgagat cggcccgcgc gacctcttca cgggtgccat
      901 cacggacgtc gagctcatca aggacaagtc gctcatcgtg aagcgcgtct tcgagcgcca
      961 tgagctcgac cgcgagggct cgatcggcgt cggcgacacg gagggcgaca tcccgctcct
     1021 ctcgtcggtc gacacgccca tctgtttcaa tccgaacatg cagctctacc agcacgcgaa
     1081 ggagaaggac tggcccatcg tggtcgagcg aaaagatgtc gtctacacca tctagacgag
     1141 gggtgacgaa actcgcggcc ttcaggagac gggtccgagg attctatgaa cgagagggga
     1201 ggcatgcgct cccgtggcga acgacgcgcg acccgtatcg catcctcgtc tccgaggtca
     1261 tgctccagca gacgcaggtc gagcgcgtca taccgaagta cgaggcgttc ctaaagcgct
     1321 tcccgaccgc gcggtccctc gcgaaggcgc gtctcggcga tgtgcttcgc ctgtggcagg
     1381 gtctggggta caaccgtcgc gcgaaactcc tccatgatgc ggcgaaggtg atcgcgtcga
     1441 agcgaagctt cccacgaacg ctcgaagagc ttcgcgcgct ccccggcgtc ggaacgtata
     1501 ccgcggcggc ggtcatgacg ttcgcgttcg acgaaccagt acccctcatc gagacgaacg
     1561 tgcgcacggt gttcatacac cacttcttcg cggatgagga cggagtcgat gacgatgcgc
     1621 ttatgccgct catcgagcga acgctcgacc gtacgagacc tcgcgagtgg tactacgcgc
     1681 tcatggacta cggctcgcac ctcaaaaaga ccgtcgggaa cgcgagcgcg agaagcgtgc
     1741 accacgtgcg ccagagcgcc ttcaagggct ccgacaggga agtgcgcggc gcaatcgtcc
     1801 gaacgctcgc ggccgggccg aaacaacgag agacgctcca tgcctcgctc ccgttcgacc
     1861 tccagcacat cgatgaacaa ctcgagcggc tcatcgagga aggcatggtc gtgcgtcgtc
     1921 gcgcagcgta cgccctaccc tgactatcga tatagcgaaa acgccccgcg gaagcggggc
     1981 gtttctgttt ctccgtaatc gatactgatt agatgccgat gaggaccggt atgaccatcg
     2041 gggtcttgtt cgtcttctgg aggaggaatc cggagacgac gttcgtgatc tcgtctttta
     2101 cgtagtcgag gtcaatgggg tgcgacttcg cagtgtactt ctcgatcgtc ttcttgacga
     2161 gaacgcgcgc ctcggagaga agctgctggt tctctcgcag gtacacgaag ccgcgcgaga
     2221 tgatatcagg cgacttccga agagcaccgg tcttcgtgtt caccgtcgcg atgatgacga
     2281 acatgccgtc ctcggcgagc gactggcggt cgcggagaac gacctcctgc ttcgcgccga
     2341 ccgtgaagcc gtcgaccatg acgatctccg acggcgcctt ctgcttatgc acgtggagct
     2401 cggttccgtt cttgatgtcg atgaccgagc cgttgtccgc gaggataacg cgttccttcg
     2461 ggaaaccggc ctcgatgttc gcgtacgcgt gacagcggag catcgagtgg tacccgtacg
     2521 cgggcatgac gaactgcggg ttcacctggg cgcgtatcca gacgagctca cccgagttac
     2581 cgtgtcccgt cgagtgcacg tccgacgcgc ggtagtggat gatgtgaacg ccgtgacggt
     2641 acaggttgtc cttgagcttc tgcaccgaga gctcgttacc cgggatgacc gaggacgaga
     2701 gcatgatggt gtcgcgctca ttgagcgtga ggaatttgtg gcggccggtc gcgatgcgca
     2761 taagcgccgc gaactcctcg ccctgggcgc ccgtcgcgag gacgacgatg cggtcggacg
     2821 ggtagttgcc gatctcctga atcgggatga tggtgccctt ctgaaccttc atgaggcccg
     2881 cgagctccgc gatctcgagg ttcgtcttga tggagcgtcc ctcgaggacg atcttcttcc
     2941 cgagctcctc gcagatcatg atgatgcgga tcatgcgctc gaactgcgac gcgaacgtgc
     3001 cgacgatgag gcggcccttc acgtttctga tgatctgttc gatgttcgtg tggacgcgcg
     3061 tctcggggat agagaagccc tcgcgctcgg cgttcgtcga atcgccgatg aaaaggatgt
     3121 tcttatcctt gccgacgccg ccccagacgc gcttctcctt ctcggacggc tcggtgccgt
     3181 cgtgctcgag tttaaggtcg cccgagatga cgatgttgcc gtgcttcgtc tctaccgaga
     3241 ctcccatcga gtcgggaatg gagtgcgtga cggggaaggg tttgatgtac gtgtttccga
     3301 tcttgatgcg ctggtcgagc tcgacgacgt tcatgttcac cttcgggagg tgcgggaact
     3361 cttcttggcg cttcaatatc atgagcgacg tgaggtagcg cgtgtagatg ggcggattgc
     3421 cgatgcgctc tatgaggaac gggatgccgc cgatgtgatc gaggtggccg tgcgtgatga
     3481 cgatgccgcg gatcttatgc ttgttctcct cgaggtactg cgtgttcggg agaatgtagt
     3541 tcacgcccgg tgcgtcgctc tcggaggaca cgaactggaa gcccgcgtcg aaaatgagaa
     3601 tgtcatcggc cgtttcgacg accgtcatgt tcttgccgac ctcctcgacg ccgcctaatg
     3661 gcacgatgcg gacgacgtcc ggatcggtgt tggggaggat gcgcggaccc ttcgcgtcga
     3721 ccggggcgat gcgatgcgtg agcgcggggc tcgttcgcgc gcggttccgt tcgaggcgac
     3781 gcctgttctg cgatggtcgt cggcgtttct cgccttgagg cgactgcccg tcacgcgggg
     3841 tgcgcgctcc gtccgccgag aacgtgcttc ggcgcccgcc gcggcgattc gcgccaccgc
     3901 gaccgcgtcg cgacgggcga cccgacgggc gacctgagaa gttcgccgcg cggcgctcgc
     3961 tattaaattg gtttgattgc tgttccataa ctggttccta atgctttacg ttgtactaat
     4021 attgatatgc tacttcgtga atatgggcca taccgagtcc gtcttgatgt accaatcggt
     4081 gaccccgctc caccgttcct gcggctccgc gagcccggtg aaggtcgcac cggtcacgtc
     4141 gttcgggaag atgtagagga gctcggggct atagaggaac accgcgggct tctccttgtc
     4201 gagctcggcg acgaacgcgt cgatcgcgtc gtttcggctc gccgtatcgg tgctcgtccg
     4261 catgtccgcg agtatcgaat cggtcgtcag gttcgcgtag agcgcgatgt tgaggcctgg
     4321 atcgtttcgc tgggacgaat gccagaacga gtagaagtcg aacgcgcggc cgagttgcat
     4381 gccgaagagg agcgcctggt agtctcgcgg tcggatgacc gattgtgcga ggtccgtttg
     4441 ttcgaactgc ttgacctcga ccgctgcgcc gagctgctcc cacgcgttct tcaggaactc
     4501 cgcggtcgcc tcgaacgtcc cgccgttcgc cgtcgagagc gagaaggaga gcggcgtctt
     4561 caccttgtcg atggtcttct cccagacgtg cgtctcatcg ttcatctccc agccgccggc
     4621 cttgaggatg tcacgcgcgg cgtcgacgcc gacgacatcg gcgtgcggcg tcgcgctcgc
     4681 gttcgtcccg aatccctcag ggaccgggcc gaagagcggc cgtccgtacc cgccgaggac
     4741 cgcatcgatg agcgcactcc ggtcgatcgc cgcgtcgagc gcctttcgcg ccgctgggtc
     4801 gcgaagcgcg gcggatttgt tctgatcgaa gaagacggcg aaggttcggg ggagcggaat
     4861 ccgctcgacg tggctcgtcg ccgagttcac ctggtacgcg gcgaggtggt cgggatcgac
     4921 gcccgcgacg ctccccacgg tgcccttctt gaacgcttga atgagcttgt cctcggacgg
     4981 atagaagtgg agctcgatgg tctttatctt cggcgacccg cggtggtatg cgtcgttcgc
     5041 tttgagcacg tacgtctccg ggatgccgga ggcgttacgc gcgatgctct cgacctcgta
     5101 gggacccgag ccgaccggct cgctgttgta ttggctgaac ggaaactcgt cggagcccgc
     5161 atccttccag atatgttcgg ggaggatacc aaacgtgagg ttctcgatga agggcgcgta
     5221 cggctgagcg atgacgaaat tgagctcgta gtctcctaag acctcgaccg agacgccatc
     5281 gaaattggcg cgaagcgggc tcgaaaggag tgggtcctcg atgcggccga cggtgaacgc
     5341 gacgtccttc gcggtgagcg gctgcccatc gtggaacttc acgtcatcgc ggaggaccac
     5401 gttgtacgtg aggccgtcat cggagacggt gacgctcttc gcgatattgg ggacgagcgt
     5461 gccgtcctct ccaagccgca tgagtccgtc gtaaacgagg gcggatagat cacggtccgc
     5521 gcgggtaacc gcgaggaccg ggttcgcaaa ccgtggtgtc ccgacgatgc cctcggagaa
     5581 cgttccgccc gtcgccggga tgaggacgct gtggtcgacg ctcgcgcgta taaggaagac
     5641 ggcgaggaac gcgaccgccg cgaggagcgc acccttgagg agaaagccgt cgctcgcctt
     5701 catgcgatcg aggcgaagga acatccggtc aacgagcgga cgtttctccc gcggggagct
     5761 ttggaatgga tccatggagt ggaaacgtta ttgctaatga gcgtgtctga aaaggcaaaa
     5821 tcggtctacg aacatgaaac gcgactgcgg cgaaacgccg ccgcttcgat tagatgagga
     5881 agctcaagaa ggtcgatgcc acgaagagga tggcagtcgc tatcgagaag cggaagagga
     5941 agagttccgc tccacgccgc ttgtggtacg tcgcactgaa gttgtcccct ccgaacgcgc
     6001 cgccgagcga tgagccccgc tgctggagca gtatcgcgga tacgagcagt atggagagga
     6061 taatttgtac gtacggtaat acggtacgga cgatttccat gtcggagaga tactagcaca
     6121 aacggcacga attggaaagc gggcaagaag
//
LOCUS       DASTSP010000037         4362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1197349, whole genome shotgun sequence.
ACCESSION   DASTSP010000037 DASTSP010000000
VERSION     DASTSP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197349"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..814
                     /gene="murI"
                     /locus_tag="VFS75_01540"
     CDS             41..814
                     /gene="murI"
                     /locus_tag="VFS75_01540"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU4677383.1"
                     /translation="MKIGFFDTGLGGLTILRAVRKRMPGYDYLFFGDTVNVPYGGKSE
                     AEVFELTKAGVIRLFKAGAAIVIVACNTASAESVRKLQDSILEGVFRDRKILGVIIPT
                     IEELHAMGAEHALLIGTERTVKSEKYTRELLRIAPAVVLEARATPALVPLIESGEIDA
                     AAHLVDDVLAHVKGRVDTLVLGCTHYTVLKERLKKTHPQLRIISQDDVIPAKLEAYLK
                     RHGEIERKLSRRGTITITESGANLHVSDFKRKLLEELGE"
     gene            complement(1107..2435)
                     /locus_tag="VFS75_01545"
     CDS             complement(1107..2435)
                     /locus_tag="VFS75_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU4677384.1"
                     /translation="MHHRVRNTNSRRLLICLAIAGTIFASGAVLRSTALAQSQIDELR
                     SQIADRNNRLAEIEKEIATYQSELKKVGGQKATLQAAIDRLELERKKVQADIRYTENK
                     IGATDLELSKLGLEIKSTETGIELNRQAIAETLRSLEETDQDSMVEVLLSNDNLAAFW
                     KKIDDLEEIRSVIADQVSTLESEKAALGEKYDLSSKKRADLVDLKSQYADQNEVLVGN
                     KAEKSKLLAATKNQETEYQKLLKAKQAEADKIQKELRDFESQLKFILDPNTVPTRGTA
                     VFRWPLDSVIITQLFGGTEFAQQNPGVYGRPYHNGVDISAPIGTPIHAALSGVVRATG
                     NTDAVAGCYSWGKWTLIDHANGLSTLYAHQSVIKVVPGQSVSTGDVIGYTGNTGYTTG
                     PHLHFTVYAKAGVSIKQFSDFKAVTSCGAATTPVAAPEAYIDPMAYLPKP"
     gene            complement(2449..3813)
                     /gene="gltX"
                     /locus_tag="VFS75_01550"
     CDS             complement(2449..3813)
                     /gene="gltX"
                     /locus_tag="VFS75_01550"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU4677385.1"
                     /translation="MSIFERQKVVTRMAPSPTGHLHIGTARTALFNYLFAKRHGGVFI
                     LRTEDTDKERSKKEFEDEIIEGLDWLGITWDAFYRQSERAAIYRGYLEKAIENGKAYV
                     SKEESKMHPGTEVEVVRLKNPNTTITFMDKIRGEITFDTTELGDFVIARSLDDALYHF
                     TVVVDDHEMEVSHVIRGEDHISNTPRQILIQEAIGAERPVYAHLPLILAPDRSKLSKR
                     HGAVMLSEYRRQGYVRSALVNYLALLGWNPGTDRELYELDELVESFDLSGIQKSGAIF
                     DIEKLKWFNKEHMRTWSDAAYFDYVVERIPERVTSLPEWSEERLRRLIPVIRERTNVG
                     DEITADCMAGEYDFAFAAPKPDPALLKWRNDATPRQTLPRLQKLAELVARIPESLEPE
                     EIKSYIWDYALEAGKGEVLWPLRVALTGRERSPDPFTVIHVIGSAEAYKRIKVACDTI
                     LMAE"
     gene            complement(3823..>4362)
                     /locus_tag="VFS75_01555"
     CDS             complement(3823..>4362)
                     /locus_tag="VFS75_01555"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4677386.1"
                     /translation="TGGSQGAERVNQLILDSLDELLPDYTIVHQTGKANFEVTRLSAE
                     TLITNESLKAYYRPTPFLDAATLNDVYGTADIIVSRAGSTSIYEIALHGKPAILIPIP
                     EEISHDQRSNAYAYARAGAAVVLEEANVSDALLRAEIDRIMQDKQLYDEMAADAREFA
                     LPDSALKISALVMEVAREH"
BASE COUNT          787 a         1256 c         1351 g          968 t
ORIGIN      
        1 cgccagacgt tctcaaagcg ctcgtaagca tcaccggcgt atgaaaattg ggttcttcga
       61 tacaggtctc ggcggtctca ccattttgcg ggcggtgcga aagcgtatgc cggggtacga
      121 ctacctcttc ttcggcgaca cggtgaacgt gccctacgga gggaagagcg aagcggaggt
      181 gtttgaactc acgaaagccg gcgtcatacg cctcttcaaa gcgggtgccg cgatcgtcat
      241 cgtcgcctgc aatacggcct cggcggagtc ggtacgaaaa ctgcaggatt cgatcctcga
      301 aggcgtcttc cgcgatagga agatactcgg cgtcataatc ccgaccatcg aggaactcca
      361 tgcgatgggc gcggagcacg cgctcctcat cggtaccgaa cgaacggtga agtcggagaa
      421 gtacacgcga gagctgctcc ggatcgcacc tgcggtcgtc cttgaggcga gagcgacgcc
      481 ggcgctcgta ccgctcatcg agtccggtga gatcgacgcg gcggcacatc tcgtcgacga
      541 cgtgctcgcg catgtgaagg gaagggtgga tacgctcgtt ctcggctgta cgcactacac
      601 ggtcctcaag gagcgactca aaaagacgca cccgcagctt cggatcatat cgcaggacga
      661 tgtgattccc gcaaagctcg aggcgtacct aaagcgacac ggagagatcg agaggaagct
      721 ctctcgaagg ggaacgatca cgataaccga gagcggtgcg aatttgcatg tgtcagattt
      781 caagaggaag ctcctcgagg agctcgggga gtagtggttt gctaaatgta ctacgtgtcg
      841 cacaatatat cttttgcgac ctattatata ctgggccact tccctacata tcgaaattta
      901 aaaagaccgc acacgcggtc tttatctcaa caaatcggtg gagtgagggt ctccgcgtag
      961 tctcgtgccg tgccactctc ctctaccgga ccggatgatc gttctaggaa tgcgcaaaga
     1021 tgccgatctc cccgacgagg acctttccaa gtatgtagat tcccgtgatg agcgcgacga
     1081 tgagaatgaa tcgtagcata ctctctctag ggctttggta ggtacgccat cgggtctatg
     1141 tacgcctcgg gtgccgctac cggtgtcgtc gccgcgccac agctcgtcac ggctttgaag
     1201 tcgctgaact gcttgatgga gacgccggcc ttcgcgtaga ccgtgaagtg gaggtgtggg
     1261 ccggtcgtgt agccggtgtt accggtgtaa ccgatgacgt cgccggtcga aacgctctga
     1321 cccggtacga ccttgatgac gctctggtgc gcgtacaagg tcgaaagccc gttcgcgtgg
     1381 tcaatgagcg tccacttgcc ccacgagtag catccggcga ccgcatcggt attgcctgtc
     1441 gcccgaacga caccggagag cgccgcgtga atgggcgtac cgatgggggc gctgatgtcg
     1501 acgccgttgt ggtacggtcg tccgtagacg cccggattct gctgtgcgaa ctccgtgccg
     1561 ccgaagagct gggttatgat gacgctgtcg agcggccacc taaagaccgc cgtgccgcgc
     1621 gtcggcaccg tgttcgggtc gagtatgaac ttcagttgcg actcgaagtc ccggagttcc
     1681 ttctgtatct tatccgcctc cgcttgcttc gccttgagga gtttctggta ttcggtctcc
     1741 tggttcttcg tcgccgcgag gagcttcgac ttctccgcct tgttgccgac gagaacctcg
     1801 ttctggtcgg cgtactggga tttgaggtcg acgaggtcgg cgcgcttctt cgagcttagg
     1861 tcgtacttct cgccgagcgc cgccttctcg gactcgagcg tcgatacctg gtccgcgatg
     1921 acggagcgta tctcttcgag gtcgtctatc ttcttccaga aggccgcgag attgtcgttc
     1981 gaaaggagta cctcgaccat cgagtcctgg tcggtctcct cgagcgagcg gagcgtttct
     2041 gcgatggctt ggcgattgag ctcgatcccc gtctccgtgc tctttatctc gagcccgagc
     2101 ttcgaaagct cgaggtcggt cgcgccgatc ttgttctcgg tgtatcggat gtccgcctgc
     2161 accttcttac gctcgagctc gagacgatcg atggccgctt gtagggttgc cttctgcccc
     2221 ccgaccttct tgagctcgct ctggtaggtc gcgatctcct tctcgatctc ggcgagtctg
     2281 ttgtttcggt cggctatctg actccgaagc tcatcgatct gggactgcgc gagggcggtc
     2341 gaacggagaa cggcgcccga cgcgaaaatg gtccccgcga tggcgaggca tatcaaaaga
     2401 cgtcgagagt tcgtattgcg gaccctgtgg tgcattgcga tatacggcct attcggccat
     2461 caatattgta tcacaggcga cttttatcct cttgtacgct tctgcggagc cgatgacgtg
     2521 gatgaccgtg aaagggtccg gtgagcgctc tctcccggtt aaggcgacgc gaagcggcca
     2581 gagaacctcg cccttccccg cctcgagcgc gtagtcccag atgtaggact tgatctcctc
     2641 cggttccagg gattcgggaa tgcgcgcgac gagttcggcg agcttctgga gccgcgggag
     2701 cgtctggcgg ggggtcgcgt cgtttcgcca cttgaggagc gcagggtcgg gtttaggcgc
     2761 cgcgaaggcg aagtcgtact cccccgccat gcagtcagcc gtgatctcat cgccgacgtt
     2821 cgttcgctcg cggatgacgg gaatgaggcg tcggagccgt tcctcgctcc attcgggaag
     2881 cgaggtgacg cgctcgggaa tgcgctcgac gacgtagtcg aagtacgctg cgtcgctcca
     2941 cgtgcgcata tgctccttgt tgaaccactt caatttctcg atatcgaata tggcaccgct
     3001 tttctggatg ccggagaggt cgaacgactc cacgagctca tcgagttcgt agagctcccg
     3061 gtcggtgccg ggattccatc cgaggagcgc gagataattg acgagggccg agcgcacgta
     3121 accctgtctc cggtactcgg agagcatgac cgcgccgtgc cgcttcgaga gcttcgagcg
     3181 gtcgggcgcg agtatcaagg gaaggtgcgc gtagacgggc cgctccgcgc cgatcgcctc
     3241 ctgaatgagt atctggcgcg gcgtgttcga gatgtggtcc tccccgcgaa tgacgtgtga
     3301 gacctccatc tcgtggtcat cgacgacgac cgtgaagtgg tagagcgcgt cgtcgagcga
     3361 gcgagcgatg acgaaatcac cgagctcggt cgtgtcgaac gttatttcgc cgcggatctt
     3421 gtccatgaag gtgatcgtcg tgttcgggtt tttgagccga acgacctcga cctccgtgcc
     3481 gggatgcatc ttgctctcct ccttgctcac gtacgctttg ccgttctcga tcgccttctc
     3541 gaggtatccg cgatagatgg cggcccgttc cgactggcgg tagaaggcgt cccacgtgat
     3601 gccgagccaa tcgagcccct cgatgatctc gtcctcgaac tccttcttgc ttcgctcctt
     3661 atcggtatcc tcggtgcgga gtatgaaaac accgccgtgt cgcttcgcga agagatagtt
     3721 gaaaagcgcg gtacgcgcgg tgccgatgtg gaggtggccg gtcgggctcg gcgccatgcg
     3781 cgtcacgacc ttctgcctct cgaaaattga catagtatcc gtttagtgtt ctcgtgctac
     3841 ctccatgacg agcgcgctta tcttcagggc gctatcgggc agtgcgaact ccctcgcgtc
     3901 ggccgccatt tcgtcgtaga gctgcttgtc ctgcatgata cggtcaattt cggcccggag
     3961 caaggcgtcg cttacgttcg cttcctcgag gacgacggcc gcaccggcgc gcgcgtacgc
     4021 gtacgcgttc gaccgctggt cgtgacttat ctcctccgga atgggaatga gaatggcggg
     4081 tttgccgtgg agcgctatct cgtagatcga cgtgctcccc gcgcgcgaca cgatgatgtc
     4141 agcggtgcca tagacgtcat tgagcgtcgc ggcatcgaga aacggcgtcg gccggtagta
     4201 cgccttgaga ctctcgttgg tgatgagcgt ctccgcggag agtctcgtga cctcgaagtt
     4261 cgccttcccc gtttggtgaa cgatggtgta atcagggaga agctcatcga gagagtcgag
     4321 tatgagctgg ttcacgcgct ccgctccctg ggaaccaccg gt
//
LOCUS       DASTSP010000038        28825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_976084, whole genome shotgun sequence.
ACCESSION   DASTSP010000038 DASTSP010000000
VERSION     DASTSP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 28825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28825
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976084"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VFS75_01560"
     CDS             <1..1305
                     /locus_tag="VFS75_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU4677387.1"
                     /translation="GEEALAERGFKVITTLDWELEEKGEEIAKKYALKNEKTYNASNA
                     SIVAADPSTGQILAMVGSRDYFDKEIDGAYNIALAERQPGSAFKPFVYAEAFKKGYYP
                     ETVVFDLRTQFSTACKPDNFTSEGNCYSPDNYDHKFRGPVSLRNALAQSLNVPAVKVL
                     YLAGLNDSLKLARDMGITTLGDAGHYGLTLVLGGGEVRLVDMVGAYGAFANEGVKNEL
                     QSILRVEDSSGNVIEEFKPESKRVLDRDVTLQIADVLSDNVARTPLYGPNSPLYFPGR
                     DVAAKTGTTNDKRDAWVLGFTPNLVVGAWAGNNDNSPMSEISGLVVTPLWRAFMDEAL
                     KKVEPKSFAQAPRTPDTAKPVLRGVWFDLSGFASADVNGLTSSSMNLLNAVAGAHSIL
                     YFVDKSDPGGPQPSNPNDDPQFPLWEYPVSIWKQQMLQSAGY"
     gene            complement(1309..1641)
                     /locus_tag="VFS75_01565"
     CDS             complement(1309..1641)
                     /locus_tag="VFS75_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677388.1"
                     /translation="MRLPNDARGNGSEPKRRRGTGIVKVGSLFEKYRKTLRAPQKSVI
                     AAFCAALEELFEIRLSPDRCSYNPRSRTLSVQASGMVKTKVAIEKRHILNYLRETLGE
                     RSAPKDIL"
     gene            complement(1638..2729)
                     /gene="dnaN"
                     /locus_tag="VFS75_01570"
     CDS             complement(1638..2729)
                     /gene="dnaN"
                     /locus_tag="VFS75_01570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU4677389.1"
                     /translation="MHIQIDTKVFRETLDVVSKISTKHSTLPVLQCVLLEVKGTELTL
                     KATNLEIGIEGTLSVTSDEDGTIAVPALILLQTVNLVNQSTLTLSTEGDLLVVEAKAS
                     RTEIKSIPAADFPSIPRVSGTPFTVSGPLFTLGVKTTAFTASQSSIKPELGSIYIFQK
                     KEHSLTFVATDSFRLMEKTVPQKNVSLGESILIPYKNALELARVTEGASGDVELFINE
                     NQCALRVGKIYITSRLVHGTFPDYEQIIPKEYKTNISLLTGDLSQALKKTSIFLNKFM
                     QLTLTAGKGALTLSAWSGEAGATTESVPANVDGEEITLNFNQRYITEALPHVQDDSVI
                     LKCAGVGRPMVIENAHDRSLRYLVMPMNK"
     gene            complement(3016..4395)
                     /gene="dnaA"
                     /locus_tag="VFS75_01575"
     CDS             complement(3016..4395)
                     /gene="dnaA"
                     /locus_tag="VFS75_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU4677390.1"
                     /translation="MSSISNIATSIIQTEQIDVKKLWDDVLVNIELSITSANFKTWFK
                     DTHIVSLEDGTVTLGVPSVFVRDWIADKFQGLILKTLRELTPHARSLEYVVVQRNERK
                     QEASKKQTVNAALPLEEYYINKSDNLNPKYTFDSFVVGPFNELAHAASITVVNKPGIA
                     YNPLFIYGKTGHGKTHLIQAIGNQLKRGGKKVFYVTSERFTVDYMNALQNGTANGFKD
                     KYRQYDVLIMDDVQFLMNKEKTQEELFHLFNAMHDNNKQIVFSSDVHPALMSNMEARL
                     QSRFSQGMIVDIPQPDIESRSAILRSKAAQNTLTLSDDVVDHLANTIEGNIRELEGAL
                     NTILCQSQLLGRALSLDEVKLLIKNSTRPRKSLAVSDVVDKVARYFDIEPASIYEKTR
                     RKEVVKPRQLIMYILREDFQVSYPAIGQKLGGRDHTTVIHSCEKIKRELKEDPELEEE
                     VSQVRLLLK"
     gene            4622..4759
                     /gene="rpmH"
                     /locus_tag="VFS75_01580"
     CDS             4622..4759
                     /gene="rpmH"
                     /locus_tag="VFS75_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU4677391.1"
                     /translation="MPKRTYQPKKRKRATTHGFLVRSRTKSGAKVLAKRRRKGRAKLS
                     V"
     gene            4773..5102
                     /gene="rnpA"
                     /locus_tag="VFS75_01585"
     CDS             4773..5102
                     /gene="rnpA"
                     /locus_tag="VFS75_01585"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU4677392.1"
                     /translation="MLPKKERLSREDFSRFFSSGKRRNSPALGLVYTPYPSLHVSVVV
                     PKKVARRAVDRNVMRRRIYDIVRRHRDEVGATGVFIFLAKAPALRMPFGALRDEVIGL
                     ITAACGR"
     gene            5118..5858
                     /locus_tag="VFS75_01590"
     CDS             5118..5858
                     /locus_tag="VFS75_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEU4677393.1"
                     /translation="MSAIWHTIFFNPIYNSLVYFIDAIPGGDVGLAIICTVILVKTII
                     LPISLKAARTQLVMRELEPKLAEIKETHKDNREIQALKTMELFKEAKVNPLSSIVLLF
                     VQIPIVIALYFAVSRGGGVPLPEINTAILYSFVPTPETVNMIFLGFLDITKKSLALAA
                     LAGFTQFLHTHLSLPPHKPREESAAPSFKDDFTRSMHLQMRYVMPVLIGFVAYTLAAS
                     VALYFTISNIMAIVQEFVLRHKGLKAKH"
     gene            complement(5891..7069)
                     /locus_tag="VFS75_01595"
     CDS             complement(5891..7069)
                     /locus_tag="VFS75_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677394.1"
                     /translation="MKRPLIIAIIALIVILLIGAWVYLFVHGSPKSAGDVFTRFGFGD
                     APPPEMPGTPTVDVAPVDASGTPERLKQLTTRPVAGAAFTAGGIEYVEQGTGHVYSID
                     LGSGEEKLESGTTIAGAERATFSGDGTYVAVTSLQNGEPNTDVGRIDGLSGKLEGVSL
                     PPGARDIGFGAASTSLYYLLPGSAGSSGYAYDLIEGKGAEMFHIPLRDVHPIWGEPLY
                     VYTTPTAHATGYVYRLDDGKLTYVHDGATGLTALPYPGGIVTTFARSGRVTSVALAAE
                     GETTLDEPLIPEKCASTPAWGSVLYCGIPDSLDAADFPDAWYMGTLSYRDSLYRVDVA
                     LGTSELVSDMLAESGREVDVLTMGASADGSKVYLINKNDNTLWMYDLGNRAEETATST
                     "
     gene            complement(7071..7724)
                     /locus_tag="VFS75_01600"
     CDS             complement(7071..7724)
                     /locus_tag="VFS75_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677395.1"
                     /translation="MTRRRHSILTIGSLAAFLFVLTTTLVFAADDGKKQYVPLVGIPG
                     LTDKQNPSIAAYINSIYLFTITVGAIIGVIKIGMAGVKYSMSDVITDKSEARKDILGV
                     FMGLAILLIPFVVLNTIYSGLTNLDVLENKNVKVDLKPSGGADPSTQTSGQDFIASLP
                     KPPFDSNGICQEGYDSSSADGASCATTCADQCASLGGTATRISTDPATGCGKIRCLK"
     gene            complement(7754..8731)
                     /locus_tag="VFS75_01605"
     CDS             complement(7754..8731)
                     /locus_tag="VFS75_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677396.1"
                     /translation="MRRSLILATLSVAFFTFSVFFTSPGHAFAQLAPSLVTLLKAPAF
                     PAPHESVEVSLSDYAVDTVGADIFWYVDGAELPNTRNARSVIVTAGAIGETKTVRADL
                     VRKNAPTLSAATVIRPAVVDLVLEAATYVPDFYRGRALPSDGAPIRAVAVVNDGTDAP
                     ESSYTYKWSESGDVLLGGPVKGKNVLDLTMTRYRKETLSVEVINDDGDTIGKRTIPLV
                     SAEPTLIFYEHSPLRGLLERAMSDVNPLIGDEMTVYGEPYFLDARTTRNDGDFTWTID
                     GTEAPADGAPNALTLSKEGTTGSASIDLRIVTKRAIPQLLRGGFTIFFD"
     gene            complement(8755..9426)
                     /locus_tag="VFS75_01610"
     CDS             complement(8755..9426)
                     /locus_tag="VFS75_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677397.1"
                     /translation="MAENARENADERDRARGIENATSRFKREHEARLTSADLIRKAEA
                     RRGGTVPEGERARADDGAARPRASSRRREEEAALADELRALSDAGIAYDEGDERLLLD
                     TPPDTPSFPGVILVVAAVKDILDGLDVTGIGVVVATALSFLMSLVLFLWTLSRQQGGW
                     WKQKGSRSTWSRYAGATIVEFIPFLQMLPAESLYVVMTYVSESKLGRSLQSALERARM
                     AKGKE"
     gene            complement(9429..11222)
                     /locus_tag="VFS75_01615"
     CDS             complement(9429..11222)
                     /locus_tag="VFS75_01615"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU4677398.1"
                     /translation="MGLFDFIQQKKTEAVGTAADAVLSLPDVIAPSAISINPRSINVS
                     GKLSRVFYAVSYPRYLNDGWLEPVLNMEREIDVSIVIHPIDTAETLKKFQKKVAEVQS
                     QINERAEKGMVRDPQLDAAYRNLEELRDKLQQAEEKLFNVGLYIAIYADTEADLNKVE
                     TEIRGILDTRLVYIKPALFQQEDGFMSVIPTVTDKLLVHSKFNSSPLSSFFPFTSFDL
                     TSDTGILYGINRHNSSLVLFDRFKLTNYNSVTFATSGAGKSYTTKLEILRSLMFGTEV
                     IVIDPEHEYEYLAEATGGRFFDISLSSTHHINPFDLPIPLPDENPKELLRSHIIELVG
                     LFRLMLGGLSPEEDSIIDAALHEVYALKDITGSGDYRDLPPPLLSDFEMVLAGMEGSE
                     SLVQRLQKYTSGTWSGFMNEPTNVDINQKFVVFSLRDMEEELKTIAMYIITNHIWGEV
                     RRELKKRLLVIDEAWWMLKSEDTAAFLHNIAKRGRKYYLGLSTITQDVDDFLRSPYGV
                     PMITNSSIQLLMKQSPTAVDTLQSVFNLTDEEKFMLLESGVGEGIFFVGLKHVAIKVI
                     ASYTEDQIITSDPSQLLRIKEAKKELGSDAA"
     gene            complement(11225..11839)
                     /locus_tag="VFS75_01620"
     CDS             complement(11225..11839)
                     /locus_tag="VFS75_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677399.1"
                     /translation="MAKQNASQQFVPVRDIHDDVVVLKSGQMCMVLLASSVNFALKSQ
                     DEQQAILSQYQAFLNSIDFSVQIYVQSRRLDIRPYLEILRAREGEQYNDLMRIQLREY
                     MEFVSSFTEQVDIMTKNFFVIVPYTPVNLEVGGISKFLKPKTTAARDDKFFEDRTQLE
                     QRVSVVEQGLARIGIRTAPLGTEELVELFYHIFNPEDLTKAPQR"
     gene            complement(11832..12242)
                     /locus_tag="VFS75_01625"
     CDS             complement(11832..12242)
                     /locus_tag="VFS75_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEU4677400.1"
                     /translation="MRFEVPQFIEIEDKIIGPLTWKQFVYVAGGVGILVILWVSLPTL
                     LFLLFGLPFGILAGSLAFHRVNNRPFSIFLESFLSYAGKGKLYLWRKDAEQSVIERTD
                     TSDVAVPKLEFANRNSIHELSRKLETQIPADPHG"
     gene            complement(12295..12765)
                     /locus_tag="VFS75_01630"
     CDS             complement(12295..12765)
                     /locus_tag="VFS75_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677401.1"
                     /translation="MTITNDMRMVALQKISPSLQEAYRSVETARLLKVLMTENGIQNF
                     DFVDLVGDVMLGFYSKSQLRSLLMQQIGLSEEVASNVDNKLRIFFSRVEGVPIASPEA
                     REVKERLEFRPEPPVGPLGRKPLTREEVLRAIAPRRTMQSDIESVKQSKGPGAA"
     gene            complement(12769..14844)
                     /locus_tag="VFS75_01635"
     CDS             complement(12769..14844)
                     /locus_tag="VFS75_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEU4677402.1"
                     /translation="MPTSAHADVLGFGSMAIDAIVSFIGNIFLSFSAVITWMGGQLFE
                     YALKFTVFEMGTRLGSGTPFGDTISNLWSLIRDICNLAFIFGFIYVGIRTIIDPDSAD
                     TKRFLSRIIVGALLINFSLFFVKAIVDVSNFTSVQIYNSLTTGTGQISETLTSQLGII
                     TIYNAKGGTILPELFASQKSFSFFLLGGIFMLITAFVFFAAALLLVVRFVAIVLIMVA
                     SPVLFAATVFPQTEKFASDLWSKLMSYSFFAPLFLLLMLISIKMIDGLKLGISAGAGG
                     SKDGFADVIAAGSNNFSTILNFTVIIFFMIAALMIASSMSGKGGKFAMGIANTARGKV
                     QSIVGRNTVGRASHYLKEKYEHLDAEASKSSWKSLRGAGLKTARFIAAAPAGGDKGVR
                     EALHHGEEAKFGGSDSYESVKKYDKERAARQANINKISELQEATDKATGVKLHGIPRL
                     AATSFANDQERIKFERMVAEATIKDFEEMEQGQREAIVAFMTQNQVDGLMKSDKLTDK
                     EKADLGAKRAKHLAELYIDNKQLLSKANADQLDALGQAYLSEEEHAVRLTGSQMDDLK
                     KKLTPTELKLVSDARENALAKLATGTTINTKEKGEPKTLDSSFILSMKPGDMAKLPKK
                     VLKPLATKLPVQVLDKIRADGTLSIGDQKEIREAFMDQYKSVESADSDPTYDWLTTSP
                     YGKQFGVGV"
     gene            complement(14917..16803)
                     /locus_tag="VFS75_01640"
     CDS             complement(14917..16803)
                     /locus_tag="VFS75_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677403.1"
                     /translation="MMRFLRKKKLVSAAVAGFLTVSLLATPVSLSTLRPQKAEAQFAV
                     IDITNYIANYGSWIADAASEAYNAVSSFAANYLQIKESTLDSIAWQLVNAVIKEMIKS
                     TTDWVNHGFKGNPTFVQDINKFTRDVADQAVGNFIWKSDDLQFLCSPFALDVKIALDT
                     QYRDTRNYQAQCTLTDVVKNVDGFLNGDFLAGGWDGWFQMTQTPSNNPYGALLETSGV
                     LQAKVAGNQDRQLALLSFGNGFMSMKDPTCKPSPDNPDGFDESACPIVTPGTVIEDQL
                     NNVLHKPGERLVVADEVNELLSALFSQLVSNVFSSAGGLAGLNDSGSGSYFDRLDHET
                     DPTVPDQGSLSVFDNPIKEETEYRDLVRQEISMLLDAQTYKDRTYGDGNSCHSEDIPD
                     DLIEALATARTALSVSESAIGRLNEFRSDFLLLQSSTTPQTALDLLYAKYNANSTPDA
                     KTTLMSRFIAYQSSGALHSPSDTVTLRIGTIRSLADEIAGLKDDIDHDCDGRTPTPGA
                     TTQYSLTPADGNKSVAANGSEVTAFLVNVPSGTKRTTLTLAKTGATPSGVSYAAWPSD
                     TAGGDAFAPFVSWPSAGGTYTLCRNASATDTSCIATSTPSSFFFNVRPTDMTSYTIRV
                     NLSN"
     gene            complement(16800..17639)
                     /locus_tag="VFS75_01645"
     CDS             complement(16800..17639)
                     /locus_tag="VFS75_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677404.1"
                     /translation="MLIILGAFHLRSASGDEPAGGAVVVKAPERAAIPIEDTDGDGVA
                     DWKDGLEAQVFSAIATPTPLSAGEDATYTPPTTFTGKFSEAFFADYLDGKIKGEDFKD
                     PSKLVGNAVSAINANTRSKTHSRSELTTTPTSPESVRAYGNALGDVLKAHSRNGDDEM
                     TILEKALSTNDASLLDALGPIRDEYAGTIADALRMSVPDDLVAEHLAFLNAVEGLKTD
                     VEAASVGLTDPLYTLARVRGHKDVQTALGTSFENIAKKLTAYGATYGADESGGFFYLF
                     LKS"
     gene            complement(17774..18586)
                     /gene="rsmA"
                     /locus_tag="VFS75_01650"
     CDS             complement(17774..18586)
                     /gene="rsmA"
                     /locus_tag="VFS75_01650"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU4677405.1"
                     /translation="MPENPPSITAKKSLGQHFLNNPNIPRKMADAASVGAGDTVLEIG
                     PGTGVLTRDLLSRGATVVAIEADRRAIDALQETFPGEIKAKKLVIFHDDARSLVLDDY
                     DLPKAYKVVANIPYYLSGLLFRAFLAAAHQPSCLVFLVQREVAERIARDSKESILSLS
                     VKVFGEPRYVETVRKGNFTPAPKVDSAIIAVTDISRERLGDLSEALFFEVVRAGFASR
                     RKQLLGNLTALLPREELIHIFSTLGIPENVRGEDLSLDGWLALASAVSVHPK"
     gene            complement(18623..20566)
                     /locus_tag="VFS75_01655"
     CDS             complement(18623..20566)
                     /locus_tag="VFS75_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU4677406.1"
                     /translation="MHDEHHLEGLNAAQKNAVLTTEGPLLVLAGAGSGKTRVITHRII
                     HLILTGTAPERILAVTFTNKAAKEMHERAMDLLRRYRGSERASWDAVPTVTTFHALGV
                     RMLREHHEVLGIKKHFTIYDRSDSSAAMKKALVRAGYDPKEFEPRRMLSMVSRAKGDA
                     ILPAEFLEKAKSFPARVAGEAWKHYEDILREEHALDFDDLLSKTLAMLRNHPPILASY
                     RERYAYVHVDEYQDTNRVQFEIARLIVGERGNLCVVGDVDQNIYSWRGADVKNILQFE
                     RHFPAAKVIMLEENYRSTQTIIEASNDVIEKNVNRPEKKVFTRNHPGELISLYAGMGG
                     ADEAEYVAGTAKELIKNGANPSDIAVLFRTNFQSRVLEEAFIAYEVPYQMLGTKFFER
                     KEVKDVLSYLRYAMNPDSTADLARIINTPARGIGKVTVLKVVEGKAETLPRATREKVE
                     AFEKIIEDIRAYAAEHPVHETIAFIMRHSGIEESYKKDGEEGQERLENVREVVSLGTK
                     YAELPPEEAVEALLEDSALGSDQDELKEREEFDAVRLMTIHAAKGLEFAYVFITGLEE
                     GLFPHERLDAEGVDEEEERRLFYVALTRAKKKVFLSYAHMRTIFGSQRVNVPSSFLSD
                     IRSELIESANPGESGFERTIWLD"
     gene            20948..21976
                     /locus_tag="VFS75_01660"
     CDS             20948..21976
                     /locus_tag="VFS75_01660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4677407.1"
                     /translation="MTYGSVKRLVLELIAIYGVVAVPIVNAGVLSLFNREAAAAPTET
                     AFEENQSAQFVPLLRAATHSDPNPAKGGGDIIVEDGALVPAGAALSDQKSTAKSNNGE
                     ISLYTVREGDTLSQIAEMFDVSAKTILWANNLSSANNIHPGDELVILPITGVRHVVKK
                     GDTLQTIAKKYDGDVDDIIAYNQLSSASDISVGETLVVPNGVISTPTPAPVLASHPTS
                     QARGVSTGNGSSGFINPVPGSVKTQGIHGYNGVDLAAPIGTHIRAAAGGTVIVAKTSG
                     WNGGYGLYVVIRHPNGVQTLYAHMSKVAVTVGQQVAQGELIGYVGSTGRSTGPHCHVE
                     VRGARNPF"
     gene            complement(22031..22399)
                     /locus_tag="VFS75_01665"
     CDS             complement(22031..22399)
                     /locus_tag="VFS75_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677408.1"
                     /translation="MSRTNIIWVLILAIIVVVIAIVFFMKSPATTPADTAAIGTTTTT
                     VAPETVESYVTANLDDLIAEATTSTSSATASYDITGYEAHGGAGTVRYTDGTTTHTAD
                     FTYNVDERGYPSVTSFTIRQ"
     gene            complement(22482..24002)
                     /locus_tag="VFS75_01670"
     CDS             complement(22482..24002)
                     /locus_tag="VFS75_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU4677409.1"
                     /translation="MSVARVFGAQPSVTGADIVTIETDLSRGLFSFAVVGLPGKAVEE
                     ARDRIGSAIKNSGFKSPKSENHKVVISLAPADLKKEGPLFDLPMALSYLIAAGDMVAD
                     VSKTVCIGELSLDGELRPVRGVLPIVRRAKELGFKECIVPETNAAEAALIPNIRVTPA
                     RTLMGVVRHLDLKREDHEPLPEAPVTQIPNLWDEGTVRLEDVRGQESAKRGLMIGAAG
                     RHNVIMVGPPGTGKTMLARAFQTILPPLSTQEALEVTSIHSIAGTGEGISSRPPFRSP
                     HHTASYTALVGGGTQPRPGEVTLAHRGVLFLDEFPEFERRAIDALRQPLEDRVVSISR
                     VHSNALFPADFILVAAMNPTRAGDERGDYAAQMLETYRHKISGPILDRIDLWLSVPHV
                     DFGTLTERREPVTKTSETDEARAKIARARERQHARFAGTGIHTNGEMTARDIEERVEL
                     SSSVADLLRRSAEKLGLSPRSYHRLVKVSRTIADLEDSNDIKESHVLEALHFRVKA"
     gene            complement(24006..24377)
                     /locus_tag="VFS75_01675"
     CDS             complement(24006..24377)
                     /locus_tag="VFS75_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU4677410.1"
                     /translation="MRDNIETYRAKLDELLRDVVTELKSVGIHNPENPADWEAVPEGD
                     IEEPDPNDHADVVEGWDERRGIVAALEGRYNSIVAALGRIHDGTYGECVVCGRPIEDE
                     RLDANPAARTCIEHREEDALT"
     gene            complement(24419..26203)
                     /locus_tag="VFS75_01680"
     CDS             complement(24419..26203)
                     /locus_tag="VFS75_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677411.1"
                     /translation="MAEETVRQNEETELGITFHTKAPEPAASAPADKKQKDVIDEMAA
                     TAEVAKKAGVFDVSKAESAEHELGTIVTDRKLHRATLGESMQSAFGEWWHNLRTSAKP
                     VIENLKKPDVGPTVAKVETRVDVIENAAKHATFAPKDDHHAVVEKIKTFKRDVARATG
                     AAFVVKEERRTEDGAPRWTHTLDERKQPALRREPHPDLAPDLRKTMVAPVVEKRIKKD
                     PSDYAKRPKTQPSILRKSASPTEVAFMHIPGTVERKNVPKPTPAQIAPVVRGGAKEEP
                     TKHEASPTREQVGYVAREEVKRAAAHVEPPAIPVFGAEALSPKKGLDTTPAPAFHAGT
                     PVSQNVEASAEPAAPKKSSRVYTTPSTSWYESHARLTWSVASGVLVIAAVLFIVLRSG
                     GPSPAANPTPGEGGTGTVPAVTSVPSFFDVDAAVPIALTDDRSGFFGALQSAVASAQT
                     GVTQFYPTITEVGVTRVAGSAEIFTTLGLSLPASATRSLDPVIMFGSVTVAKNEPFVV
                     LRGKNFDSLFAGLLAWEPRLRSDLSPLFDTSYVERDVFEDAVKQGRAARVLHDALGSE
                     VLLYTFVDDTTVVITSSEEALTKVMARL"
     gene            complement(26270..28405)
                     /locus_tag="VFS75_01685"
     CDS             complement(26270..28405)
                     /locus_tag="VFS75_01685"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU4677412.1"
                     /translation="MEKRSYSIDVSGKALTATFTNLTGQAHGSVLMSMGETVVLVTAV
                     MSERTMSGMTYFPLSVEFEEKFYATGQILGSRFIRREGRPSDGAVLSARIVDRTIRPL
                     FDHALRNEVQVVATVLAAGEDDPDVLAVIGASLALSVSDIPWDGPVGAVRIGKRRDTG
                     AIVVNPTYPEREEGILTYEVLACGKDGGINMIETAGFEIAEADVVEALEQAVVLNTEL
                     EAWQARIIAEIGKTKRAIALPTLSPEAIALFNEKIAPKLGSTLFSNRSGKTHLYELKD
                     EWLTLLRETIADADQGLAGEYFEHALDDELHRGIIDDNVRPDGRGMDEVRPLFAQAGG
                     VSPALHGSGIFYRGETHIFTALTLGGPDDSQVLDTIEENGTKKQFMHHYNFPPFSVGE
                     TGRVGGQNRRMIGHGALAEKALVAVIPPKTDFPYTIRLVSETMSSNGSSSMGSVCAST
                     LALMDGGVPIARAVAGIASGVMMRDHDHYKLLTDIQGPEDEHGDMDFKVAGTRVGITA
                     MQMDVKVAGVPVRILAEALEKARLARLHILDTLEATIAAPRPDISPRAPKILVLTVKP
                     DQIGLVIGSGGKTINGIKEETGVDEITIEDDGTVYITGRGDAPEKARERIADITRTYE
                     VGERLEVEITRITTFGAFAKMNAHTEGLIHISEIVPWRLESLDGILTVGEKVTVVVSK
                     VEGDKIGLSIKKAETDFLERKGLTPPERK"
     gene            complement(28545..>28825)
                     /locus_tag="VFS75_01690"
     CDS             complement(28545..>28825)
                     /locus_tag="VFS75_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU4677413.1"
                     /translation="AVDAIKYSEKVDAIVIASGDGDFVPLAEYLRSHGCQVEAIAFGK
                     SSSQRLRENVDDFIDMDQTPQQFLIGRRGGVSRHRRTVSRVVGADTND"
BASE COUNT         5256 a         8551 c         8993 g         6025 t
ORIGIN      
        1 ggggaggagg cgctcgccga acgcggcttc aaggtcatca cgaccctcga ctgggagctc
       61 gaggagaagg gtgaggagat agcgaagaag tacgccctca agaacgagaa gacgtacaac
      121 gcgtcgaacg cgtcgatcgt cgccgcggac ccatcgacgg gacagatcct cgcgatggtc
      181 ggctcacgcg attatttcga taaggagatc gacggggcgt ataacatcgc gctcgccgag
      241 cgacagcctg gttccgcgtt caagccgttc gtgtatgccg aggcgttcaa gaagggatac
      301 taccccgaaa cggtcgtctt cgacctccgc acgcagttct cgaccgcctg taagcctgat
      361 aacttcacct ccgaggggaa ctgctactct ccggacaact acgaccacaa gttccgcggc
      421 cccgtgtcgc tccgaaacgc gctcgcgcag tccctcaacg ttcccgccgt gaaggtcctc
      481 tatctcgccg gactgaacga ctcattgaag ctcgcgcgtg acatgggtat cacgaccctc
      541 ggcgacgcgg gccactacgg cctcacgctc gtcctcggcg gcggcgaagt gcgtctcgtc
      601 gatatggtgg gcgcgtacgg cgcgttcgcg aacgagggcg tgaagaacga gctccagagc
      661 atccttcgcg tcgaggacag ctcggggaac gtcatcgagg agttcaagcc cgagtcgaag
      721 cgcgttctcg accgcgacgt gacgctccag atcgccgacg tgctctcgga caacgtcgcg
      781 cgaacgccgc tctacggtcc gaactcgcct ctctacttcc ccggacgcga cgtcgccgcg
      841 aagaccggaa cgacgaacga caagcgcgac gcatgggtcc tcggctttac gccgaacctc
      901 gtcgtcggcg cgtgggcggg gaacaacgac aatagtccga tgagcgagat atcgggactc
      961 gtcgtcacgc cgctctggcg cgcgttcatg gacgaggcgc tgaagaaggt cgaaccgaaa
     1021 tccttcgcgc aagcgccgcg cacacctgac acggcgaagc cggtcctccg gggcgtctgg
     1081 ttcgacctaa gcggcttcgc atccgccgac gtgaacggcc tcacctcgtc gtccatgaac
     1141 ctcctcaatg cggtcgccgg agcgcactcg atactctatt tcgtcgataa gagcgatcct
     1201 ggagggccgc aaccctcgaa cccgaacgac gacccccaat tcccgctgtg ggagtacccg
     1261 gtctccatct ggaaacagca gatgctccaa tcggccggat attagttctt aaagaatgtc
     1321 cttcggcgca cttcgctcgc cgagcgtctc gcgaaggtaa ttgaggatat ggcgcttctc
     1381 gatcgcgacc ttcgtcttca ccatacccga cgcctgaacg gagagcgtcc gcgaccgtgg
     1441 gttgtacgaa caccggtccg gtgagagtcg tatttcgaag agttcctcga gcgccgcgca
     1501 gaacgcggcg atgacgctct tttgaggcgc acggagtgtt tttcggtact tctcgaagag
     1561 cgagccgacc ttcacgatgc ccgtgccgcg ccgtcgtttc ggttcactgc cgttaccgcg
     1621 tgcgtcgttc ggtagcctca tttgttcatc ggcatgacga ggtaccgaag cgagcggtca
     1681 tgcgcgttct cgatgaccat cggtcggccg acgcccgcgc acttgaggat gacgctgtcg
     1741 tcctgaacgt gggggagcgc ctcggtgatg taccgttgat tgaaattaag ggtaatctcc
     1801 tcgccgtcga cgttcgcggg taccgattcg gtcgtcgcgc ccgcctcacc gctccatgcg
     1861 gagagcgtga gcgctccctt ccccgcggtg agcgtgagct gcatgaactt attgagaaag
     1921 atgctcgtct tcttcagggc ctgcgagagg tcgcccgtga ggagcgagat gttcgtttta
     1981 tactccttcg gaatgatctg ctcgtagtcg ggaaacgtcc catgaacgag ccgcgaggtg
     2041 atgtagatct taccgacgcg aagcgcgcac tggttctcgt ttataaagag ctcgacgtcg
     2101 cctgacgctc cttcggtaac gcgtgcgagt tctagcgcgt tcttatacgg gatgagaatg
     2161 gattcaccga gcgagacgtt cttttgcggt acggtcttct ccatgaggcg gaacgagtcc
     2221 gtggcgacga atgtgagcga gtgctccttt ttttggaaga tgtagatgct cccaagctcc
     2281 ggcttgattg aagattgcga cgcggtgaac gcggtcgtct tcacaccgag cgtgaagagt
     2341 gggccgctta cagtgaaggg ggtgccggag acgcgcggaa tactcgggaa gtcagccgcg
     2401 ggtatgctct ttatctccgt ccgtgacgcc ttcgcctcga cgacgaggag gtcgccctcc
     2461 gtcgagagtg tgagcgtcga ttggttcacg agattcaccg tttgaagcag gataagcgcg
     2521 ggaacggcga tcgtcccatc ctcatcggag gtgacggaaa gggttccctc gatacctatc
     2581 tcgaggttcg tcgccttcaa tgtgagctcc gtccctttca cctcgaggag tacgcactgg
     2641 agtacgggga gtgtgctgtg cttcgtactt atcttcgaca ccacgtcgag cgtttctcgg
     2701 aagaccttcg tgtcgatctg tatgtgcata atcgtttatt tcttttttaa gaattcctca
     2761 tactcactac tattacgtat gtggatgtgt gtataggtcc actatacttc cattttcact
     2821 cgtttttcat aaacccactc ccgtacagac ctgtagatag cggcgcggac aacgatgtat
     2881 cgaattaatc ggttcttcat gaagtccgga ttaagaaccg aatgaagagc ggattaagtc
     2941 cctatccgca cgacgtccac aactgaccgc ggcgcgtttt ccacaaggtc atcacggggt
     3001 aaagaacagc tacgactact taaggagaag tcgcacctgc gatacctcct cctcgagctc
     3061 tgggtcctcc ttaagctcac gctttatctt ctcgcatgag tgaatgacgg tcgtatggtc
     3121 gcggccgccg agcttttgtc cgatcgccgg gtacgatacc tggaaatcct cacggaggat
     3181 gtacatgatg agttggcgcg gcttcacgac ctccttacgg cgcgtcttct catagatgct
     3241 cgcgggttcg atgtcgaagt accgcgcgac cttgtcgacg acgtcggata cggcgagcga
     3301 cttccgcggt cgcgtgctgt tcttgatgag gagcttcacc tcatcgaggg agagcgcacg
     3361 gccgaggagc tgggattgac agaggatcgt attgagcgct ccctcgagct cgcggatgtt
     3421 cccctcgatg gtgttcgcga ggtggtcgac gacgtcgtcg cttaacgtaa gggtattttg
     3481 cgccgccttc gagcgcagga tggcgctccg agactcgatg tcgggctgtg ggatgtcgac
     3541 gatcattccc tgtgagaaac gagactggag gcgtgcctcc atgttcgaca tgagggctgg
     3601 gtgaacgtcc gacgagaaca cgatctgctt gttgttgtcg tgcatcgcgt tgaagaggtg
     3661 gaagagctcc tcctgcgtct tctccttgtt catgaggaac tggacgtcgt ccatgatgag
     3721 cacgtcgtac tggcggtact tatccttgaa cccgttcgcg gtgccgttct ggagcgcgtt
     3781 catgtagtcg acggtgaagc gctcgctcgt cacgtagaag accttcttcc ctcctcgctt
     3841 cagctggttc cctatcgcct ggatgaggtg cgttttcccg tgacccgtct ttccatagat
     3901 gaagagcggg ttgtacgcga tacccggctt gttgacgacc gtgatcgacg ccgcgtgcgc
     3961 gagctcgttg aacggcccga cgacgaaact gtcgaacgtg tacttcgggt tgaggttgtc
     4021 gctcttattg atgtagtact cctctaaggg aagtgcggcg ttcaccgtct gcttcttcga
     4081 tgcctcctgc ttacgctcgt tccgctggac gacgacgtac tcaagcgagc gcgcgtgagg
     4141 cgtgagttcc cgaagcgtct ttaaaataag cccctggaac ttgtccgcga tccaatcgcg
     4201 gacgaagacc gaggggactc cgagggtgac cgtaccatct tcgagtgaga cgatgtgcgt
     4261 gtccttgaac catgtcttga agttcgcgga cgtgatggag agctctatat tgacgagtac
     4321 gtcgtcccat aacttcttca catcgatctg ctcagtttgg ataatgctgg tagcgatgtt
     4381 tgatatggaa ctcatacctc tagatagctg cggataacat gtagcattat acgtccctgt
     4441 ggaaaactgt aaaccgaacg cgcaaatgcg cggtttccgc ggcgggagcg cacagagcgt
     4501 gtgcacaacg tgtggagagc aatgtatgcg cgaaaggtca atggtcttcg gcgaacgcga
     4561 acgatattct tgaaatcgcc gaaaatgtag tgtagagtgt ggcgatagcg aatattcatc
     4621 tatgccaaag cgaacctacc aaccgaagaa gcgcaagcga gcgacgacgc acgggttcct
     4681 cgtccgctcg aggacgaagt ccggtgcgaa ggtgctcgcg aagcgccgcc gcaagggccg
     4741 cgcgaagttg agcgtataag ccgcaaaacc gcatgttgcc gaaaaaagaa cgattgagca
     4801 gagaggactt cagtcgtttt ttttcgtctg ggaaacggcg gaactccccc gccctcggac
     4861 tcgtctacac gccctatccg tcgctccacg tctcggtcgt cgttccgaag aaggtcgcgc
     4921 gacgcgccgt cgaccgcaat gtgatgcgaa ggcgaatcta tgatatcgtg agacgtcacc
     4981 gggacgaggt cggcgcgacg ggagtgttca tcttcctcgc gaaggcgccg gcccttcgca
     5041 tgccgtttgg cgcgctccgc gacgaggtca tcggcctcat cactgcggcc tgcggccgat
     5101 aaccacccta tacatatatg agcgcgatct ggcataccat cttcttcaac ccgatctata
     5161 atagtctcgt ctacttcatc gacgcgatac cgggcggcga cgtcggtctc gccatcatct
     5221 gtacggtcat cctcgtgaag accatcatcc tccccatctc tttgaaggcg gctcgcacac
     5281 agctcgtcat gcgcgagctc gaaccgaagc tcgccgagat caaggagacg cacaaggaca
     5341 atcgcgaaat acaggcgcta aagacgatgg aactcttcaa ggaagcgaag gtaaacccgc
     5401 tttcgagcat cgtactcctc ttcgtccaga taccgatcgt catcgccctc tacttcgcgg
     5461 tctcacgagg cggcggcgtt ccccttcccg agataaacac cgccatcctc tactcgttcg
     5521 tgccgacgcc cgagacggtc aatatgatat tcctcggatt cctcgacatc acgaagaaga
     5581 gtctcgcgct cgcggcgctc gccggcttca cgcagttcct ccacacgcac ctctcgcttc
     5641 cgccgcataa gccgcgcgag gagagcgccg cgccgagctt caaggacgac ttcacgcgga
     5701 gcatgcacct ccagatgcgg tacgtcatgc cggtcctcat cggcttcgtc gcgtacacgc
     5761 tcgcggcgtc ggtcgcgctc tacttcacca tcagtaacat catggcgatc gtccaggagt
     5821 tcgtccttcg gcacaagggg ttgaaggcga agcactagga ggtcgaaaaa agcgggggta
     5881 cccgcttttt tcatgtgctc gtcgcggtct cttccgctcg gttccccagg tcgtacatcc
     5941 agagcgtgtt gtcgttctta tttatgagat agaccttcga gccatccgcg ctcgctccca
     6001 tcgtgaggac gtcgacctcg cggccgctct cggcgagcat atccgatacg agttcggacg
     6061 taccgagcgc cacatcgacg cggtagagcg aatcgcggta cgagagggtc cccatgtacc
     6121 acgcatccgg gaaatccgcc gcatcgagcg agtccggtat gccacagtag aggacgcttc
     6181 cccaagcggg ggtcgaggca cacttctcgg gaatgagcgg ttcatcgagc gtggtctctc
     6241 cttccgccgc gagagcgacc gaggtaacgc gtcccgaacg agcgaacgtc gtgacgatgc
     6301 cgcccggata cggaagcgcc gtgaggccgg tcgcgccatc gtgaacgtac gtgagcttgc
     6361 cgtcatcgag tcggtagacg tacccggtcg cgtgcgcggt cggcgtcgtg tacacgtaga
     6421 gcggctctcc ccaaatcgga tgaacgtctc ggagcgggat gtggaacatc tccgcgccct
     6481 tcccctcgat gaggtcgtac gcgtacccgg atgagcccgc cgaacccgga aggaggtaat
     6541 agaggctcgt cgacgccgcg ccgaagccga tatcgcgcgc cccgggcgga agcgacacgc
     6601 cctcgagctt cccggagaga ccgtcgatgc ggccgacgtc ggtgttcggt tcgccgtttt
     6661 ggagcgacgt cacggcgacg tacgttccgt caccggagaa cgtcgcgcgc tccgcgcccg
     6721 cgattgtcgt gccgctctcg agcttctcct cgccgcttcc gaggtcgatc gagtagacgt
     6781 gtcccgtccc ctgctcgacg tactcgatgc cgccggccgt gaacgcggcg cccgctaccg
     6841 gccgcgtcgt gagctgtttg aggcgctcgg gtgtccccga ggcgtcgacc ggcgcgacgt
     6901 cgacggtcgg cgtgccaggc atctccggag ggggtgcgtc accgaagccg aagcgcgtga
     6961 agacgtcgcc cgcgctcttc ggcgacccgt ggacgaagag gtacacccat gcaccgatga
     7021 ggagaatgac gataagcgcg atgatggcga tgatgagcgg acgcttcata tcatttcaaa
     7081 caccggatct ttccgcaccc ggtcgcgggg tcggtgctta ttcgtgtcgc ggtaccaccg
     7141 agcgacgcgc attggtccgc gcacgtcgtc gcgcacgagg ccccgtccgc ggagctcgag
     7201 tcatagcctt cttggcatat cccgttactg tcgaacggcg gcttcggaag gctcgcgatg
     7261 aagtcttgac cgctcgtctg cgtcgacgga tccgctccgc cggaaggctt gaggtcgacc
     7321 ttcacgttct tattctcgag gacatcaagg ttcgtgaggc cgctgtagat ggtattgagg
     7381 acgacgaacg ggatgaggag gatggcgagg cccatgaaga cgccgaggat gtccttccgc
     7441 gcctcggact tgtcggtgat gacgtcgctc atcgagtact tcacgcccgc catgcctatt
     7501 ttgatgacgc cgatgatcgc ccctacggtg atggtgaaga gatagatcga gttgatgtac
     7561 gcggcgatag atggattttg cttgtcagta aggcccggga taccgacgag cggcacatac
     7621 tgtttcttcc cgtcgtcggc tgcgaagacg agcgtcgtcg taaggacgaa gagaaacgcc
     7681 gcgagcgaac cgatcgtgag gattgaatgt cgtcgtcgtg tcatgtctct attataaacc
     7741 ccgaacggtc ggcttaatcg aagaagatgg taaagccgcc gcggagaagt tgcgggatgg
     7801 cgcgcttcgt cacgatgcgg aggtctatcg acgcgcttcc cgtagtgccc tcttttgaaa
     7861 gcgtgagcgc gttcggcgcg ccgtccgcgg gggcctccgt gccgtctatc gtccacgtga
     7921 agtccccgtc atttcgcgtc gttctcgcat cgaggaagta cggctcgccg tagacggtca
     7981 tctcgtcgcc gatgagcgga ttcacgtcgc tcatcgcccg ttcgaggagg ccgcggagcg
     8041 ggctgtgctc gtagaagatg agcgtcggct ccgctgaaac gaggggaatg gttcgcttgc
     8101 cgatcgtgtc gccgtcgtcg tttatgacct cgacgctcaa ggtctctttt cggtagcgcg
     8161 tcatggtgag gtcgaggacg ttcttcccct tcaccggtcc gccgaggagg acatcgccgc
     8221 tctcggacca cttgtacgtg tacgaactct cgggggcatc ggtaccgtcg ttcacgaccg
     8281 cgacggcgcg tatcggcgca ccgtcgctcg gtagcgcccg cccgcggtag aagtcgggga
     8341 cgtacgtcgc cgcttcgagg acgagatcga cgaccgccgg tcgtatgacc gtggccgccg
     8401 agagggtcgg cgcgttcttt cggacgaggt cggctcgtac ggtcttcgtc tcgccgatcg
     8461 ctcccgcggt cacgatgacg ctccgcgcat ttcgggtgtt cgggagctcg gcgccgtcta
     8521 cgtaccaaaa gatgtccgcg cccacggtgt cgacggcgta gtcgctcaag gacacttcga
     8581 cgctctcatg gggcgccggg aacgccggag ctttaaggag ggtcacgagg gacggtgcga
     8641 gttgcgcgaa ggcgtgtccc ggggacgtga aaaagaccga aaatgtgaag aatgcgacgg
     8701 aaagagtggc gagaatcaac gaacgtctca tatatatgta taagtgtagc acggctactc
     8761 tttgcctttc gccatgcgtg cccgctcgag cgcgctctgg agcgagcggc cgagcttcga
     8821 ttcacttacg tacgtcataa cgacgtagag ggactccgcg gggagcatct ggaggaacgg
     8881 tatgaactcg acgatggtcg cccccgcgta gcgcgaccac gtcgagcgcg aacccttctg
     8941 cttccaccag ccgccttgct ggcggcttag cgtccagagg aagaggacga gggacatgag
     9001 gaacgagagc gcggtcgcga cgacgacgcc gatgccggtc acgtcgagcc catcgagaat
     9061 atccttcact gcggcgacga cgaggatgac gccgggaaac gaaggcgtat cgggcggcgt
     9121 atcgagaagg agtcgctcgt ctccctcgtc gtacgcgata ccggcgtcgg aaagcgcgcg
     9181 aagctcgtcg gcgagggccg cctcctcctc acgccggcga gagctcgccc gcggtctcgc
     9241 tgcaccatca tccgctcggg ctcgctctcc ctccggaacc gtccccccgc ggcgcgcctc
     9301 cgccttccga atgaggtcgg cgctcgtgag gcgcgcctcg tgctcgcgct tgaagcgcga
     9361 ggtcgcgttc tcgatgccgc gcgctctgtc gcgctcgtcg gcgttctctc gtgcgttctc
     9421 tgccatggtt atgcggcgtc gctaccaagc tccttcttcg cctccttgat gcgcagaagt
     9481 tgcgatgggt cggacgtgat gatctggtcc tcggtgtacg aggcgatgac cttgatcgcg
     9541 acgtgcttga ggccgacgaa gaagataccc tcgccgacgc ccgactcgag gagcatgaac
     9601 ttctcctcgt cggtgagatt gaagacgctc tggagcgtat cgaccgcggt cggcgactgt
     9661 ttcatgagga gttgtatcga cgagttcgtg atcatcggca cgccgtaggg cgagcgaagg
     9721 aaatcgtcga cgtcctgcgt gatcgtcgag aggccgaggt agtacttgcg tccgcgcttc
     9781 gctatattgt ggaggaacgc cgcggtgtcc tccgacttga gcatccacca ggcctcgtcg
     9841 atgacgagga ggcgtttttt gagttcccgt cgcacctcgc cccagatgtg gttcgtgatg
     9901 atgtacatcg cgatggtctt cagctcctcc tccatatcgc ggagcgagaa gacgacgaat
     9961 ttttggttga tatcgacgtt cgtcggctcg ttcatgaagc ccgaccacgt tcccgacgtg
    10021 tacttttgga gccgttgcac gagcgattcg ctcccttcca tgccggcgag aaccatctcg
    10081 aagtcggaaa gtaagggcgg cggaaggtcg cggtagtcgc ccgaacccgt tatgtccttg
    10141 agcgcgtaga cctcgtggag cgccgcatcg atgatggagt cctcttccgg cgaaaggccg
    10201 ccgagcatga gcctgaagag cccgacgagt tcgatgatgt gcgaacgaag gagctccttc
    10261 gggttctcgt cggggagcgg tatcggaagg tcgaacggat tgatgtggtg cgtcgacgag
    10321 agcgagatgt cgaagaagcg cccgcccgtc gcctcggcga ggtactcgta ctcgtgctcg
    10381 gggtcgatga cgatgacctc ggtcccgaac atgagggagc ggagtatctc gagcttcgtc
    10441 gtgtacgact ttcccgcgcc ggaggtggcg aacgtcaccg agttgtagtt cgtgagcttg
    10501 aagcggtcga agaggacgag ggaactgttg tggcggttga tgccgtagag gatgcccgtg
    10561 tcggacgtga ggtcgaagga cgtgaatggg aagaacgacg agaggggcga cgagttgaac
    10621 ttgctgtgga cgaggagttt gtcggtgacc gtcggaatga cgctcatgaa cccgtcctcc
    10681 tgctggaaga gcgccggttt gatgtacacg agacgcgtgt cgaggatgcc tcgtatctcc
    10741 gtctcgacct tgttgaggtc ggcttccgtg tccgcgtata tcgcgatgta gagtccgacg
    10801 ttgaagagct tctcctccgc ttgctggagc ttgtcgcgaa gttcctcgag gtttctatac
    10861 gcggcgtcga gctgcgggtc gcggaccatg cccttctcgg cgcgctcgtt gatctggctc
    10921 tgcacctcgg cgaccttctt ctggaacttc ttcagtgtct ccgcggtgtc gatcggatga
    10981 atgacgatgg agacgtctat ctcgcgctcc atgttgagga ccggctcgag ccagccgtcg
    11041 ttgaggtagc ggggatacga gaccgcgtag aagacgcggg agagcttccc gctcacgttt
    11101 atggagcgcg ggttgatgct gatcgcggac ggtgcgatga cgtccgggag cgagagtacc
    11161 gcgtcagccg cggtgcccac cgcctcggtc ttcttctgtt gtatgaaatc gaaaagtccc
    11221 atggtcaacg ttgcggcgcc ttcgtgaggt cctccggatt gaagatgtgg tagaagagct
    11281 cgaccagttc ctcggtcccg aggggcgccg tcctgatgcc gatcctagcg agtccctgct
    11341 cgacgaccga gacgcgctgt tcgagctggg tccggtcctc gaagaacttg tcgtcccgcg
    11401 ccgcggtcgt cttcggcttg aggaacttcg agatgccgcc gacctcgaga ttgaccggcg
    11461 tgtacggcac gatgacgaag aagttcttcg tcatgatgtc gacctgctcg gtgaacgagc
    11521 tcacgaactc catgtactcg cgaagctgaa tgcgcatgag gtcgttgtac tgttcgccct
    11581 cccgcgcgcg gaggatctcg aggtacggcc ggatatcgag acgccgcgac tgcacgtata
    11641 tctgaacgga gaaatcgatg gagttgagga acgcttggta ctgcgagagg atggcctgct
    11701 gttcgtcctg cgatttgagc gcgaagttca ccgatgatgc gaggaggacc atgcacatct
    11761 gaccgctctt caggaccacg acatcgtcat ggatgtcgcg gacgggaacg aactgttgtg
    11821 aagcgttctg tttagccatg gggatctgcg ggtatttgcg tctcgagctt tcgggagagc
    11881 tcgtgtatcg agttgcggtt cgcgaactcg agcttcggga ccgcgacgtc ggaggtgtcg
    11941 gtccgttcga tgaccgattg ctcggcgtcc tttcgccaga ggtagagctt ccccttcccc
    12001 gcgtacgaga ggaaggactc gaggaatatg gagaacggac gattattgac gcgatggaac
    12061 gcgagcgagc ccgcgaggat gccgaagggt agtccgaaaa ggaggaagag gagcgtcggg
    12121 agcgataccc agaggatgac gaggatgccg acgccgcccg cgacgtacac gaactgtttc
    12181 caggtgaggg gcccaatgat cttgtcctct atttcgatga actgtggaac ttcgaatcgc
    12241 atatgctcat tgtaccctac tcatggcgcg cggagccgcg gcgaccgtgg aaggtcacgc
    12301 ggcgcccggt cccttcgact gtttcaccga ctcgatgtcg ctctgcatcg tgcgccgcgg
    12361 cgctatcgcg cggagaacct cctcgcgggt gagcggcttt ctcccaagag ggccgacggg
    12421 cggctcgggc ctgaattcaa gacgctcctt cacttctctt gcttcgggtg aagcaattgg
    12481 aactccttct actcgagaga aaaaaatcct caatttgtta tctacatttg aagccacttc
    12541 ttcagagagg ccgatttgct gcatgaggag gcttcgaagc tgcgactttg aataaaagcc
    12601 aagcatgaca tcgccgacca aatccacaaa atcaaaattt tgaataccat tttcagtcat
    12661 gagtaccttc aaaagccggg ctgtctcgac cgatctatat gcctcttgaa gactcggaga
    12721 gatcttttga agggcaacca tccgcatgtc atttgttatt gtcatatgct atacgcctac
    12781 accaaattgt ttaccatatg ggctggtagt caaccaatca tacgttgggt cagaatcagc
    12841 actttcaacc gatttgtatt gatccataaa tgcctctcgt atttcttttt gatccccgat
    12901 agaaagtgta ccgtctgctc taattttatc gagcacctgc acaggcaact ttgttgcgag
    12961 tggcttgaga actttctttg gtaactttgc catgtcgcct ggcttcatac tcaagatgaa
    13021 gctcgaatcg agcgtcttgg gttccccctt ctctttagtg tttatggtcg tgcccgttgc
    13081 caactttgcg agagcattct ctcgagcgtc ggataccaac tttaattcgg tcggcgtgag
    13141 ttttttcttg aggtcgtcca tctgtgagcc ggtgaggcgt accgcgtgct cctcctccga
    13201 gaggtacgcc tgaccgagcg cgtcgagctg atccgcgttc gctttcgaaa ggagttgctt
    13261 gttgtctatg tagagttcag cgaggtgctt cgcgcgtttc gcaccgaggt ccgccttctc
    13321 cttatccgta agtttatcgc tcttcatgag gccgtcgact tgattttgag tcatgaacgc
    13381 gacgatcgct tcgcgctgtc cctgctccat ctcctcgaag tccttgatgg tcgcttcggc
    13441 gaccatgcgc tcgaatttga tacgctcctg atcgttcgca aagctcgtcg cagcgagtct
    13501 ggggatgccg tggagcttga cacccgtcgc cttatcggtc gcttcctgaa gttccgatat
    13561 cttgttgatg ttcgcctggc gtgccgccct ctccttgtcg tacttcttca cgctttcata
    13621 tgaatcggaa ccaccgaact tcgcctcttc tccatggtgt agcgcctcgc ggactccctt
    13681 atcaccgccc gcaggtgcgg cggcaatgaa tcgagcggtc ttcaagccgg caccgcgcaa
    13741 gctcttccag ctcgacttgc tcgcctcggc gtcgaggtgt tcgtacttct ccttgagata
    13801 gtgcgaagca cggccgaccg tgtttcggcc cacaatactc tgcactttac cccgagcggt
    13861 gttcgcgatg cccatcgcga acttgcctcc ctttccgctc atgctcgagg cgatcatgag
    13921 cgccgctatc atgaagaaga tgatgacggt gaagttgaga atagtactga aattattgct
    13981 ccctgccgct attacgtccg cgaaaccatc tttactccct ccggcacccg cacttattcc
    14041 aagcttgagg ccgtctatca tctttatcga tatgagcata agcaagagaa agagcggcgc
    14101 gaagaacgag tagctcatga gcttcgacca gaggtctgac gcgaacttct cggtttgggg
    14161 gaagaccgtc gccgcgaaga ggaccggcga ggcgaccatt atcaaaacga tggcgacgaa
    14221 gcgaacgacg aggaggagcg cggcggcgaa gaacacgaac gcggtgatga gcatgaatat
    14281 gccgccaagg aggaagaagg agaaactttt ctgggaagcg aacaattccg gtaagatggt
    14341 gccccctttt gcattgtata tcgtgataat gccaagttgc gacgtgagcg tctcggatat
    14401 ctgaccagtg ccggtggtta gcgagttgta gatttgaacc gatgtgaagt tcgagacgtc
    14461 gacgatcgcc ttcacgaaga agagactgaa gttgatgagg agcgcgccga cgatgatgcg
    14521 cgagagaaag cgcttcgtgt cggcactatc ggggtcgatg atggtgcgga tgccgacgta
    14581 gatgaacccg aagatgaacg cgagattgca gatgtcgcga atcagggacc ataaattact
    14641 tatcgtatct ccgaaaggag ttccggagcc gagacgggtg cccatctcga agacagtgaa
    14701 cttcagtgcg tactcgaaga gctgtcctcc catccacgtg atgaccgccg agaacgacag
    14761 aaagatgttg cctatgaaac taacgatcgc atctatcgcc attgagccaa acccaaggac
    14821 atcggcatgt gcagatgtcg gcatcaagac aatgacgagt aacgcgctca cgagccagat
    14881 cttcactatg cggtttttcg ggaagcgcgg catacgttag ttcgaaaggt tcacgcggat
    14941 ggtgtagctc gtcatatccg tcggccggac gttgaagaag aacgagctcg gcgtcgacgt
    15001 cgcgatgcac gacgtgtcgg tcgcgctcgc gttccggcag agcgtgtacg tgccgcccgc
    15061 cgagggccac gagacgaagg gcgcgaacgc gtcgccgccc gcggtatcgg acggccacgc
    15121 ggcgtacgag acgccagacg gcgtcgcgcc cgtcttcgcg agcgtgagcg tcgtccgctt
    15181 cgtgccggag ggaacattga cgaggaacgc ggtcacctcg ctcccgttcg ccgcgaccga
    15241 cttgttgccg tccgcgggcg tgagcgagta ctgcgtcgtc gcgccggggg tcggcgtcct
    15301 cccatcgcag tcgtggtcga tgtcgtcctt gaggcccgcg atctcgtccg cgaggcttcg
    15361 aatggtgccg atgcggagcg tgaccgtatc gctcggcgag tggagcgcgc ccgacgattg
    15421 gtacgcgatg aagcgcgaca taagggtcgt cttcgcgtcg ggcgtcgagt tcgcgttgta
    15481 tttcgcgtag aggaggtcga gcgccgtctg cggcgtcgtc gaactctgaa gaaggaggaa
    15541 gtcgctcctg aactcgttga ggcgaccgat cgcgctctcc gagaccgaga gcgcggtacg
    15601 cgccgtcgcg agcgcctcga tgaggtcgtc ggggatgtcc tcgctgtggc acgagttgcc
    15661 gtcaccgtac gtgcggtcct tgtacgtctg tgcgtcgagg agcatcgata tctcctgacg
    15721 gacaagatct cggtactcgg tctcctcctt gatcgggtta tcgaagaccg agagcgagcc
    15781 ctggtccgga accgtcgggt ccgtctcgtg gtcgagccgg tcgaagtacg agccggagcc
    15841 agaatcgttg agaccggcga gcccgccggc gctactgaag acgttgctga cgagctggga
    15901 gaagagcgcg cttaagagtt cgttcacctc atccgcgacg acgagacgct cgccaggctt
    15961 gtggagcacg ttgttcagct ggtcttcgat gaccgtccca ggcgtaacga tggggcacgc
    16021 gctctcgtcg aaaccgtcgg ggttgtccgg gctcggttta caggtcgggt ctttcatcga
    16081 catgaacccg ttcccgaagc taaggagcgc gagctgacgg tcctggtttc ccgctacctt
    16141 cgcctggagg acgccgctcg tctcgaggag cgcgccgtag gggttgttcg agggcgtctg
    16201 cgtcatctgg aaccacccgt cccagccgcc cgcgaggaag tcgccgttga ggaagccgtc
    16261 gacgttcttc acgacgtcgg tgagcgtaca ctgcgcctgg tagtttcgcg tatcgcggta
    16321 ctgggtatcg agcgcgatct tcacgtcgag cgcgaacggc gaacagagga attggaggtc
    16381 gtcgctcttc cagatgaagt tcccgacggc ctggtcggcg acgtccctcg tgaacttgtt
    16441 gatgtcctgg acgaacgtcg ggttcccctt gaagccgtga ttcacccaat cggtcgtcga
    16501 cttgatcatc tccttgatga ccgcattgac gagttgccac gcgatggagt cgagcgtcga
    16561 ctctttgatt tggagataat ttgcggcaaa cgatgataca gcgttgtacg cttccgaggc
    16621 agcgtcagct atccaacttc catagttggc gatgtagttc gttatatcga tgacagcaaa
    16681 ttgcgcctcg gccttctgcg gacggagcgt cgagagcgag acgggtgtgg caaggagcga
    16741 gacggtgagg aatcccgcga cggcggctga tacgagcttc ttttttcgaa ggaagcgcat
    16801 catgacttga ggaaaaggta gaagaatccg cccgattcgt cggcgccgta ggtcgcgccg
    16861 tatgcggtga gcttcttcgc gatgttctcg aaggacgtcc cgagcgccgt ctgcacgtcc
    16921 ttgtggccgc gcacgcgcgc gagcgtgtag agcgggtcgg tgagtccgac cgacgccgcc
    16981 tcgacgtcgg tcttgaggcc ctcgacggcg ttcagaaacg cgaggtgctc ggcgacgagg
    17041 tcgtccggga cgctcatgcg gagcgcgtcg gcgatcgtgc ccgcgtattc gtcgcgaatg
    17101 gggccaagcg cgtcgagaag cgaggcgtcg ttcgtcgaga gcgccttttc gagtatcgtc
    17161 atctcatcgt cgccgtttcg cgagtgcgct ttgagaacgt cgccgagcgc gttcccgtac
    17221 gcgcggacgg attccggaga ggtcggcgtt gtcgtgagct cgcttcgcga atgcgttttg
    17281 ctcctcgtgt tcgcgttgat ggcactgacc gcgttcccga cgagtttcga cgggtctttg
    17341 aaatcctccc cctttatctt cccgtcgagg tagtccgcga agaacgcctc ggagaacttt
    17401 ccggtgaagg tcgtcggagg cgtgtacgtc gcgtcctcgc ccgcagagag cggggtcggc
    17461 gtcgcgatgg ccgagaagac ctgcgcctcg agcccgtcct tccaatcggc gacgccatcc
    17521 ccgtccgtgt cctcgatcgg gatggccgcg cgctcgggcg ccttcacgac gacggcgccg
    17581 cccgccggtt cgtcaccaga ggcggaccgc aggtggaaag cgccgaggat gatgagcacc
    17641 ccgactacga ccgcgcctcc tatacgaaac gttcctttca cacgaaagtt gtatactcat
    17701 aaagtatagc aattgcgcgg ataaactgtg ggtaaaatgg cgataacctg tggttaaccg
    17761 aaggatattc tcgtcacttc gggtgcacgg agacggcgct cgcgagggcg agccacccgt
    17821 cgagcgagag gtcctcgcca cggacgttct ccgggatgcc gagtgtggaa aagatgtgga
    17881 tgagttcttc gcgcgggagg agggccgtga ggtttccgag gagctgtttc cggcgagacg
    17941 cgaatccggc gcgtacgacc tcgaaaaaaa gcgcttcgga gagatcaccg aggcgttccc
    18001 gcgagatatc ggtgaccgcg atgatcgccg aatccacctt cggcgcgggg gtgaaattcc
    18061 ccttcctcac cgtctcgacg tatcgaggtt cgccgaaaac cttgacggat aaggataaaa
    18121 tcgattcctt cgagtcgcgg gcaatgcgct cagcgacctc tctttggacg aggaacacga
    18181 ggcaggatgg ctggtgcgcg gccgctaaaa aagcgcgaaa aaggagcccg gagagatagt
    18241 acggaatgtt cgcgaccacc ttgtacgcct tcgggaggtc gtagtcgtcg agcacgaggg
    18301 aacgcgcgtc gtcgtgaaaa atgactagtt ttttagcctt tatttcccct ggaaacgtct
    18361 cctggagcgc gtcgatagcg cgccggtcgg cttcgatcgc gacgaccgtc gccccgcgcg
    18421 agaggaggtc gcgggtgaga acgccggtcc ccggccctat ttcgaggacg gtgtcgcccg
    18481 cgccgacgga ggcggcgtcg gccattttcc gcggtatgtt cggattattg agaaaatgct
    18541 ggccgagaga cttcttcgcc gtaatggagg gagggttttc gggcatacgg agagtatagg
    18601 tcctcggtac ggaaatcgaa aatcagtcga gccagatggt tcgctcgaac cctgactcac
    18661 cggggttcgc gctctcgatg agctcgctcc ggatgtcgct aaggaacgac gagggaacgt
    18721 ttacccgctg gctcccgaag atggtgcgca tgtgcgcgta cgagaggaag accttcttct
    18781 tcgcgcgcgt gagcgcgacg tagaagaggc gccgctcctc ttcctcgtcg acgccctcgg
    18841 cgtcgagacg ctcgtggggg aagagcccct cctcgagtcc ggtgatgaag acgtacgcga
    18901 actcgagtcc cttcgcggca tggatggtca tgaggcggac cgcatcgaac tcctccctct
    18961 ccttgagctc gtcctggtcg gagccgagcg cggaatcctc gaggagcgct tcgaccgcct
    19021 cctcgggtgg aagttccgcg tacttcgtgc cgagggacac gacctcgcgc acgttctcga
    19081 gtcgctcctg tccctcctcg ccgtccttct tgtagctctc ttcgatgccc gaatgtcgca
    19141 tgatgaacgc gatggtctcg tgaacgggat gctccgcagc gtacgctcgg atgtcctcga
    19201 tgatcttctc gaacgcctcg accttctcgc gcgtcgccct cggaagcgtc tcggccttcc
    19261 cttcgacgac cttgagcacg gtcaccttgc cgataccgcg tgcgggcgtg ttgatgatgc
    19321 gcgcgaggtc ggcggtcgag tcgggattca tcgcatagcg gaggtacgag agaacgtcct
    19381 tcacctcctt tcgctcgaag aacttcgtgc cgagcatttg gtacggcacc tcgtaggcga
    19441 tgaacgcctc ctcgagaacg cgcgactgga agttcgttcg gaagaggacg gcgatatcgc
    19501 tcggatttgc gccatttttt atgagttctt tcgccgtccc cgcgacatac tccgcctcgt
    19561 ccgcaccacc cattcccgcg tagagcgaaa tgagctcacc ggggtggttc cgcgtgaaga
    19621 ccttcttctc ggggcgattc acgttcttct cgatgacgtc gttcgacgct tcgatgatgg
    19681 tctgcgtcga gcggtagttc tcctcgagca tgatgacctt cgccgcggga aagtgtcgct
    19741 cgaactggag gatgttcttc acgtccgcac cgcgccagct gtagatgttc tggtcgacgt
    19801 cgccgacgac gcacaggttc ccccgctccc cgacgatgag acgcgcgatc tcgaactgca
    19861 cgcggttcgt atcttggtat tcatcgacat ggacgtacgc gtaccgctcg cggtacgacg
    19921 cgaggatcgg cggatggttc cggagcatcg cgagcgtctt cgaaaggagg tcgtcgaagt
    19981 cgagcgcgtg ctcttcgcgg aggatgtcct cgtagtgctt ccacgcctcg cccgcgacgc
    20041 gtgcggggaa gctcttcgcc ttctcgagaa attccgcggg caagatcgcg tcgcccttcg
    20101 cgcgggacac catgctaagc atgcgtcgcg gctcgaactc cttcgggtcg tatccggcgc
    20161 ggacgagggc tttcttcatc gccgcagagg agtcgctccg atcgtagatg gtgaagtgct
    20221 tctttatgcc gaggacctcg tgatgctccc ggagcatacg gacgccgagg gcgtggaagg
    20281 tcgtcacggt cggcaccgcg tcccacgacg cgcgctcgct ccctcggtat cgcctgagga
    20341 ggtccatggc gcgctcgtgc atctccttcg ccgccttgtt cgtgaacgtg acggcgagga
    20401 tgcgctcggg tgccgtaccc gtaaggataa ggtggatgat gcgatgggtt atgacccggg
    20461 tcttcccgga gcccgcaccg gcaaggacga ggagcggtcc ttcggtcgtc aaaacagcgt
    20521 tcttttgagc cgcatttaag ccctcgaggt gatgttcatc gtgcatatcc gttcgttagg
    20581 catcaactat agcatgcgac ctcggaacct ctccccaccc gttttcatag ccgcttcata
    20641 cgctgcctta ccattacaca ataaaagtgc ctgtctggga agacctaaat cgcggctcgg
    20701 caacgggcta aaatcgtctt cataaatatc ctgtggacaa cgctctttgg caaggttgac
    20761 ccccctcccc gcttggctat gatggtacac ggtgaaagtg acagttcgcc gggagcgacg
    20821 agacgtctgc tctttttagt ttcagcgata ccgtaccgga agcactgcgg agagaccata
    20881 acacacgtat ataaaacagt tctcacaaga ttctggccgc gctcataagg gcaggacgac
    20941 gcgacagatg acatacggaa gcgtgaagcg gctcgttctc gagctcatcg ccatttacgg
    21001 ggtcgtcgcc gttcccatcg tgaacgccgg cgtcctctcg cttttcaacc gcgaggcggc
    21061 cgccgctccc acggagaccg cgttcgagga gaatcagagc gcacagttcg taccgctcct
    21121 tcgggcggcg acgcactccg acccgaatcc cgcgaaggga ggcggcgaca tcatcgtcga
    21181 ggacggcgcg ctcgtcccgg cgggcgccgc gctctccgac cagaaatcga ccgcgaagtc
    21241 gaacaacggc gagatcagcc tctatacggt ccgcgagggc gatacgctct cgcagatcgc
    21301 ggagatgttc gacgtctcgg cgaagaccat tctctgggcg aacaatcttt cgagcgcgaa
    21361 caacatccat cccggtgacg agctcgtcat cctccccatc acgggcgttc gccacgtcgt
    21421 gaagaagggc gatacgctcc agacgatcgc gaagaaatac gatggtgacg tcgacgacat
    21481 catcgcgtac aaccagcttt cgtcggcgtc tgacatttcc gtcggagaga cgctcgtcgt
    21541 cccgaacggc gtcatcagta cgccgactcc cgcgccggtc ctcgcctccc acccgacgtc
    21601 gcaggcgcgc ggcgtttcga ccgggaacgg cagttcaggg ttcatcaacc cggtccccgg
    21661 atcagtgaag acacaaggca tccatggata caacggcgtc gacctcgccg caccgatcgg
    21721 cacgcacatt cgcgccgcgg cgggcggaac ggtcatcgtc gcgaagacct cggggtggaa
    21781 cggcggctac ggactctacg tcgtcatccg tcacccgaac ggcgtgcaga ccctctacgc
    21841 gcacatgtcg aaggtagccg tcacggtcgg tcagcaggtc gcgcagggcg agctcatcgg
    21901 gtacgtcggt tcgaccggcc gttcgacggg accgcactgt cacgtcgagg tgcgcggcgc
    21961 gcgaaacccg ttctaatgga aacatttaaa ccccccgacc ttcggtcgcg ggggtttttc
    22021 ctattcaccg ctactgccgt atggtgaacg atgtcaccga ggggtagcct cgttcgtcga
    22081 cgttgtacgt gaaatcggct gtgtgagtcg tcgttccatc ggtgtatcgg acggtccccg
    22141 caccgccgtg cgcctcatag ccggttatgt cgtacgacgc ggtcgcgctc gatgtcgacg
    22201 tcgtcgcctc cgcgatcaaa tcgtcgagat tcgcggtcac gtacgactcg accgtctctg
    22261 gcgcgacggt cgtggtcgtc gtcccgatcg ccgccgtgtc ggctggcgtg gtcgccgggc
    22321 ttttcataaa aaagacgatg gcgatgacga cgacgatgat cgcgaggatg agaacccaga
    22381 tgatgttcgt tcgtgacata gagaagtccg cccatcgtat cacgctcgca tgaagtttcc
    22441 atgaaacgtc ctgaacgggt cctgtaagga agagcgccga cctatgcctt cactctgaag
    22501 tggagcgctt cgaggacgtg tgattctttg atgtcattgg aatcctcgag gtcggcgatg
    22561 gttcgcgaga ccttcacgag tcggtggtag ctccgcggcg agagtccgag cttctcggcc
    22621 gagcggcgta agaggtcggc gacggaactc gagagctcga cgcgctcttc gatgtcgcgc
    22681 gcggtcattt ccccgttcgt gtgaatgccc gttcccgcga atcgcgcgtg ctggcgttct
    22741 cgtgcccgcg ctatcttcgc gcgcgcctcg tcggtctccg acgtcttcgt gacgggttct
    22801 cgccgctcgg tgagcgtgcc gaaatcgacg tgcggcacgg agagccagag gtcgatgcgg
    22861 tcgaggatgg gaccggatat cttgtgccgg tacgtttcga gcatctgtgc ggcgtagtcg
    22921 ccacgctcgt cgcccgcgcg cgtcgggttc atcgcggcga cgaggatgaa gtccgccgga
    22981 aagagcgcgt tcgagtggac gcgcgagatg gagacgaccc ggtcctcgag cggttggcgg
    23041 agcgcgtcga tggcgcgtcg ctcgaactcg gggaactcgt cgagaaagag gacaccgcgg
    23101 tgcgcgagcg tcacctcgcc cggacgtggt tgtgtgccac cgccgacgag cgcggtgtac
    23161 gaggcggtat ggtgcggtga acggaacggc ggtcgtgacg agatgccttc gcccgtcccc
    23221 gcgatcgaat gtatcgaggt cacctcgagc gcctcttggg tggagagtgg cgggaggatg
    23281 gtttggaacg cccgcgcgag catcgtcttt cccgttcccg gcggtccgac catgatgacg
    23341 ttgtgtcgtc ccgccgcacc tatcatgaga ccgcgcttcg ccgactcctg acctcgaacg
    23401 tcttcgaggc ggacggtccc ttcgtcccag aggttcggta tctgcgtgac gggtgcctcg
    23461 gggagcggtt catggtcttc gcgcttcagg tcgaggtggc ggacgacccc catgagcgtc
    23521 cgcgccggcg tcacacgaat gttcgggatg agcgcggcct ccgcggcgtt cgtttccggt
    23581 acgatgcatt ctttgaagcc caactccttc gcgcgacgca cgatggggag gacgccgcgc
    23641 accggtcgta gctcaccgtc gagcgagagc tcaccgatgc acaccgtctt cgagacgtcg
    23701 gccaccatgt cgcccgccgc gatgaggtac gagagcgcca tcgggagatc gaagagcgga
    23761 ccctccttct tgaggtccgc gggcgcgagc gaaatgacga ccttgtggtt ctcgctcttc
    23821 ggcgacttga agccggagtt cttgatggcg ctcccgatcc tatcgcgtgc ctcctcgacc
    23881 gcctttcccg ggaggccgac gaccgcgaac gaaaacagac ctcgcgagag gtccgtctca
    23941 atggtgacga tgtccgcgcc ggtcacggat ggttgtgctc cgaagacgcg tgcgaccgac
    24001 ataggctagg tgagcgcgtc ctcctcccgg tgctcgatgc acgtgcgggc cgcggggttc
    24061 gcatcgagac gttcgtcctc gatcggtcta ccacaaacga cgcactcgcc gtacgtaccg
    24121 tcatggatgc ggccgagcgc ggcgacgatc gagttgtacc gtccctcgag cgcggcgacg
    24181 attcctcggc gttcgtccca tccttcgacg acgtccgcgt ggtcgttcgg gtccggctcc
    24241 tcgatgtcgc cctcggggac cgcttcccag tcggcgggat tctccgggtt atggataccg
    24301 acgctcttca attccgtgac gacatcgcgg agtagctcat cgagcttcgc gcggtacgtt
    24361 tcgatgttgt ctctcataga aaaaagtata gcacgcgggg ctcggcgggc gatgaaggtc
    24421 ataacctcgc catgaccttc gtgagcgctt cttcggacga ggtgatgacg accgtcgtat
    24481 cgtcgacgaa ggtatagagg agaacctccg aacccaacgc gtcgtggagg acgcgcgcgg
    24541 cgcgtccctg tttcacggca tcctcgaaga cgtcgcgctc gacgtacgaa gtgtcgaaca
    24601 ggggcgagag gtcgctccgg aggcgcggct cccaggcgag gagtcccgcg aagagggagt
    24661 cgaagttctt tccgcggaga acgacgaacg gttcgttctt cgccacggtc acgctgccga
    24721 acatgatgac ggggtcgagc gagcgcgtgg ccgaggcggg gaggctaagt ccgagcgtcg
    24781 tgaagatctc ggccgaccca gcgacgcgcg tgacgccaac ctccgttatc gtcggataga
    24841 actgcgtaac gccggtttgc gccgatgcga cggcgctttg gagcgctccg aagaacccac
    24901 tgcggtcgtc ggtgagagcg atgggtacgg ccgcatcgac gtcgaagaac gacggcacgg
    24961 acgtgaccgc gggaacggtg ccagtgccac cctcaccggg cgtagggttc gccgctggcg
    25021 agggaccgcc gctccggagc actatgaaaa ggacggccgc gatgacgaga acacccgacg
    25081 cgactgacca cgtaagacgc gcgtgcgact cgtaccatga ggtcgatggc gtcgtgtaca
    25141 cgcgtgagct cttcttcggt gcggccggtt ccgcgctcgc ttcgacgttc tgcgagacgg
    25201 gcgtgccggc gtgaaacgcg ggtgctggcg tcgtgtccaa gccctttttg ggagagagcg
    25261 cctctgcgcc gaagaccggt atggcgggtg gttccacgtg tgccgcggct cgcttcacct
    25321 cctcgcgcgc gacgtagccg acctgttctc tcgtgggaga cgcttcgtgt ttcgtcggct
    25381 cctccttcgc gccgccacga acgaccggag cgatctgtgc gggcgtcggc ttcggtacgt
    25441 tctttcgctc gacggttccc gggatgtgca tgaaggcgac ctcggtaggc gacgcgctct
    25501 ttcgaagtat cgatggctgc gttttcggtc gcttcgcgta gtcgctcggg tccttcttga
    25561 tgcgtttctc gacgaccggc gcgaccatcg tctttcggag gtccggcgcg aggtcgggat
    25621 gcggctcccg ccttagcgcc ggttgtttcc gctcgtcgag cgtgtgcgtc cagcgcggtg
    25681 cgccatcctc cgttcgccgc tcctccttca cgacgaacgc ggcgcccgtc gcacgagcga
    25741 cgtctcgctt gaacgtcttg atcttctcga cgacggcgtg gtgatcgtcc ttcggcgcga
    25801 aggtcgcatg cttcgccgcg ttctcgatga cgtcgacgcg cgtctcgacc ttcgcgacgg
    25861 tcggaccgac gtcgggcttc ttgaggttct cgatgaccgg tttcgcggac gtacgaaggt
    25921 tgtgccacca ctctccgaac gccgactgca tgctctcgcc caaggtcgcg cgatggagtt
    25981 tccggtcggt gacgatggtg ccgagctcgt gctcggcgct ttcggccttc gatacgtcga
    26041 agacgccggc cttcttcgcg acctctgcgg ttgccgccat ctcgtcgatg acgtcctttt
    26101 gcttcttgtc ggcgggcgcc gacgcggccg gttcaggcgc cttggtgtgg aaggttatgc
    26161 cgagctcggt ctcttcgttt tgacgtacgg tctcctctgc catgctaccc gtagtatctc
    26221 atggatcgac gaaggccaca tgcggtcgcc cacatgtggc ctcgtaccac tacttccgct
    26281 cgggtggcgt gagtcccttc cgttcgagga agtccgtctc cgccttcttt atcgaaagcc
    26341 cgatcttgtc tccctcgacc ttcgagacga cgacggtgac cttctcgccg accgtgagga
    26401 tgccgtcgag cgactcgagt cgccacggga cgatctccga aatatggatg aggccctccg
    26461 tgtgcgcgtt catcttcgcg aacgcgccga acgtcgtgat gcgcgtgatc tcgacctcga
    26521 gccgttctcc cacctcgtac gttcgggtga tgtccgcgat gcgttcgcgc gccttctccg
    26581 gcgcgtcacc gcgtccggtg atgtagacgg tgccgtcgtc ctcgatcgtg atctcgtcga
    26641 cgccggtctc ctccttgatg ccgtttatcg tcttcccgcc cgagccgatg acgagaccga
    26701 tctggtccgg cttcacggta aggacgagta tcttcggtgc gcgcggcgag atgtcggggc
    26761 gcggcgccgc gatcgtcgct tcgagcgtgt cgaggatgtg gagtcgcgcg aggcgcgcct
    26821 tctcgagcgc ttcggcgagg atgcgaacag gaacgccggc gaccttcacg tccatctgca
    26881 tggcggtgat gccgacgcgc gttcccgcga ccttgaagtc catgtcgccg tgctcgtcct
    26941 cgggcccctg gatgtcggtg aggagcttgt agtggtcatg gtcccgcatc atgacgccgg
    27001 aggcgatacc cgcgaccgcg cgcgcgatcg ggacgccgcc gtccatgagc gcgagggtcg
    27061 atgcgcacac cgaacccatc gaactcgaac cgttcgacga catcgtttcc gagacgaggc
    27121 gtatcgtgta cgggaagtcg gtcttcggcg ggatgaccgc gacgagcgct ttctccgcga
    27181 gcgcgccgtg cccgatcatg cggcggttct gtccgccgac gcgtcccgtc tcgccgaccg
    27241 agaagggcgg gaaattgtag tggtgcatga attgcttctt cgtgccgttc tcttcgatcg
    27301 tatcgaggac ctgcgagtcg tctgggccgc ctaaggtgag cgcggtgaag atgtgcgtct
    27361 cgccgcggta gaagatgccc gaaccgtgga gcgcgggcga aacgccgccc gcctgcgcga
    27421 aaagcggccg cacctcgtcc ataccgcggc catcgggacg cacgttgtcg tcgatgatgc
    27481 cgcggtggag ctcgtcatcg agcgcgtgct cgaagtactc gcccgcgaga ccttggtccg
    27541 cgtcggctat ggtctctcgg aggagcgtga gccactcgtc cttgagctcg tagaggtgtg
    27601 tcttccccga gcggttcgag aagagcgtgc tcccgagctt cggtgcgatc ttctcattaa
    27661 aaagcgcgat cgcttcgggc gagagcgtcg gcagagcaat cgcgcgcttc gtcttaccga
    27721 tctcggcgat gatccgcgcc tgccacgcct cgagctcggt gttcaatacg accgcttgct
    27781 cgagggcttc gacgacatcg gcctccgcta tctcgaagcc ggcggtctct atcatgttga
    27841 tgccaccgtc ctttccgcac gcgagaactt cgtacgtgag gataccctct tcccgctcgg
    27901 ggtacgtcgg gttcacgacg atggcgcccg tgtcgcggcg ctttccgatg cggaccgcgc
    27961 cgaccgggcc atcccacgga atgtccgaga ccgagagcgc gagcgacgcg ccgatgaccg
    28021 cgaggacgtc cgggtcgtct tcgcccgcgg cgaggaccgt cgcgacgacc tgcacctcgt
    28081 ttcggagcgc atggtcgaag agcggtcgaa tggtcctatc gacgatgcgc gcggagagga
    28141 ccgcgccgtc cgaagggcgt ccctctcggc gaatgaagcg gctcccgagt atctgccccg
    28201 tcgcgtagaa cttctcctcg aattcgacgg agagcgggaa gtacgtcatg ccggacatcg
    28261 tccgctcgct catgaccgcg gtcacgagga cgacggtctc ccccatcgac atgaggacgg
    28321 agccgtgcgc ctggcccgtg aggttcgtga acgtcgcggt gagcgccttc ccgctcacgt
    28381 cgatactgta tgatcttttc tccatgctgc gattgcctta gcggtccgaa ttctagattt
    28441 caaacgagtc gaaatctacc tattcggacc ccttctgata acacgcccag tctagcaaaa
    28501 accggaacga aaaaacatcc gccctctggc ggatgtctct ctcgttagtc gttcgtatcg
    28561 gcgccgacga ctcggctcac cgttcggcgg tgccgtgaga cccctccgcg cctcccgatg
    28621 aggaattgct gtggcgtttg gtccatatcg atgaagtcgt cgacgttctc gcggagccgc
    28681 tgcgagctcg acttgccgaa ggcgatcgcc tcgacctgac agccgtgcga gcggaggtac
    28741 tcggcgaggg ggacgaagtc gccgtcgccc gaggctatga cgatcgcgtc gaccttctcc
    28801 gagtacttga tggcgtcgac ggcga
//
LOCUS       DASTSP010000039         7002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1201263, whole genome shotgun sequence.
ACCESSION   DASTSP010000039 DASTSP010000000
VERSION     DASTSP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201263"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFS75_01695"
     CDS             complement(<1..509)
                     /locus_tag="VFS75_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU4677414.1"
                     /translation="MPARVPEVTLKTRVRDESVEGPNPYRWQDVTTDELFKGKKVVVF
                     GLPGAFTPTCSSTHLPGYEAKYDELKAQGVDEVYCVSVNDAFVMFQWGKAQDVSKVKL
                     IPDGSGDFTRGLGMLVKKNNLGFGDRSWRYSMYVEDGEIKKAFIEPGFGDDCPTDPFE
                     VSDVDTMLT"
     gene            709..1971
                     /locus_tag="VFS75_01700"
     CDS             709..1971
                     /locus_tag="VFS75_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEU4677415.1"
                     /translation="MTTAALRERVDGLMRKNRRVAGEHMYTVPSPEHYPFQWLWDSCF
                     HAIILSHLDPALARAELRSVLAKPLASGLIPHMIYWEDSYEGVKWGREKQGDLLSAAW
                     GVHGVSSITQPPVIATAVWQLHVREPDAAFIREIYPMLRAHYLALLSERSFGPSGLLG
                     IINPDESGEDNSPRFDSLQGLPPRHDPVENFSKRIERVREHVACNFDVQNCMVHRFWV
                     EDVSFNAISIRGLEALSRIAREIGDVNDAVLFSLSAQQLRTSMQLHMKRGTRYYSLQG
                     ESEEEVIVDTWTLFMPLFAELLHPEEAKELIETALLDKRSFFTKYPVPSVSRREPSYT
                     DDDLWRGPTWAAANWFIYKGLLNYGYDDVASTIRERTIELLDRSGFREFYNAETGEGY
                     GAHEFTWGGLVLDMDGEKLGVPTSPRAS"
     gene            complement(1949..2698)
                     /locus_tag="VFS75_01705"
     CDS             complement(1949..2698)
                     /locus_tag="VFS75_01705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4677416.1"
                     /translation="MKKPAKTSWGKEAAWYAEHLTDADTYHAKVILPNIMRLVAPNKD
                     QTVLEIGCGEGYFARALSKEGSMVTAADISPELIAEAKKQGGGVAYVVSPAQDLSFAR
                     DAFFDVVLAVLTLQNMERLDLVMKEVRRVLKKEGRFLFVLNHPVIRVPKLSSWGYDEG
                     SKTQYRRLDGYLSERRTAIDMHPGEKRKSVTYSFHRSLQEYMKLLRGAGFSITRLEEW
                     ISHKTSEPGPRAKAENVARKEFPLFMMLEAM"
     gene            complement(2722..3072)
                     /locus_tag="VFS75_01710"
     CDS             complement(2722..3072)
                     /locus_tag="VFS75_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU4677417.1"
                     /translation="MTISVKRVYDAYEKKDGLRVLVDRLWPRGVTKVKAHIDVWAKEL
                     SPSTELRQWFHEEPERRYESFRTKYAKELKSEKHRAVELLKGEKNVTLVTAAKDIEHS
                     HVPTLRSFLTSLAK"
     gene            complement(3102..3647)
                     /locus_tag="VFS75_01715"
     CDS             complement(3102..3647)
                     /locus_tag="VFS75_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU4677418.1"
                     /translation="MSERAYYAVARTFHWIVAGIVGFQFLTAWIMPGVRRSTNPAYLV
                     NIHMTYGAILLPFVALFFASRFFRPASPPYRLSKLNERLAKAVHHSIYALLFLVPLTG
                     LAYATWRGWTVTIFGAYELPHLFAGSPYLRLLAELHGGLATILGYLVLGHIGAALYHH
                     FVLRDEVLVTMLPWAKRSVRG"
     gene            complement(3644..4243)
                     /locus_tag="VFS75_01720"
     CDS             complement(3644..4243)
                     /locus_tag="VFS75_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677419.1"
                     /translation="MKPHTRGNALIVVVVVIAIALIAGIYIYGVRTPSPSVDSGTESP
                     AGTSTAVTPVVPTGWKTADATSSNATFSYPESFGTTYVEAYDWPPVLTIENQSYSCTE
                     AGSPDASGGRTEKRIIDGKEYCVTTEMGGAAGSVYSQYAYATDLAGKEAILTFTVRSP
                     QCGNYEEPQKSECANEEATFDLDSLIGDIMQTIGFKPAA"
     gene            complement(4337..4702)
                     /locus_tag="VFS75_01725"
     CDS             complement(4337..4702)
                     /locus_tag="VFS75_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677420.1"
                     /translation="MMIEWNRVTWYSRIGALVLFLGIVPALAFCIGARYGAVRQQSAD
                     LDLIAPVVETPAAGSEGGTPISGKIDINAVCEGALAYMTFPDAASADAFVAECKEGKH
                     PEVIEHFKADMNLGDDAAI"
     gene            complement(4777..5382)
                     /locus_tag="VFS75_01730"
     CDS             complement(4777..5382)
                     /locus_tag="VFS75_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677421.1"
                     /translation="MFPKNSAEYKFLKKLNTPEKVQTFLEKLPFNHEKGGETCMSPKR
                     VLQTRTAHCLEGALLACAAFMLAGRKPYIVNLKVHDSDIDHAIVLFTENGRYGAISKT
                     NHAVLRFRDPVYRSLRELVMSYFHEYFLYGNGKKTLIGYSKPLNVRRFGTKWITAEED
                     LWDMGGAIFDAPITRIAPPRTRLRRAQPFERNLLKKQEWPE"
     gene            complement(5388..5783)
                     /locus_tag="VFS75_01735"
     CDS             complement(5388..5783)
                     /locus_tag="VFS75_01735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEU4677422.1"
                     /translation="MGETNEFSFVLKPAAHGVGVFATHGIAKGTHLRMFGDGETVSNR
                     SVVRNKEDVPEQFREYCADRGEKLLCPADFGQMAVGWYLNHSREANAIPDEDYKWYAA
                     RDIEAGEEITIDYNSLGEPAEAREDYYSK"
     gene            complement(5803..6072)
                     /locus_tag="VFS75_01740"
     CDS             complement(5803..6072)
                     /locus_tag="VFS75_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HEU4677423.1"
                     /translation="MHWLYQNGWNGMDMLGIGALAVFLPILVLWSIGWKGWALWLAAR
                     RHEPVWFVVLLIVNTLGILEIIYIFGIAKRKDHKEERPTTTAGSV"
     gene            complement(6139..>7002)
                     /locus_tag="VFS75_01745"
     CDS             complement(6139..>7002)
                     /locus_tag="VFS75_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677424.1"
                     /translation="PLRYASTTNVTVKGSLWPTAAPYPNGDAILPFKRIVAYYGNFYS
                     RQMGILGELEPTDMLAHLAEKVADWEKEDPTTPVLPAIEYIAIVAQGSAGADGMYRAV
                     MPDKEIDKAYELAKEIHGVLILDIQVGLSPLSTELPKFKKYLERPDVFLAIDPEFSMK
                     GGQRPGTVIGTFDATDVNYAIDYLSTIVRDNELPPKVLIVHRFTEEMVTGSSRITPTP
                     EVQVVVNMDGWGPKDLKRGTYGQVIAPEPVQFTGVKLFYKNDLKTPSTGLLTPAEVMA
                     LHPKPIYIQYQ"
BASE COUNT         1326 a         2151 c         2069 g         1456 t
ORIGIN      
        1 tacgtaagca tcgtgtcgac gtccgagacc tcgaacgggt ccgtcgggca atcgtcgccg
       61 aatcccggct cgatgaacgc cttcttgatc tcgccgtcct cgacgtacat actgtagcgc
      121 cacgagcggt cgccgaatcc gagattgttc ttctttacga gcatgccgag gccgcgcgta
      181 aagtctcccg acccgtccgg aatgagctta accttcgaca cgtcctgcgc cttgccccac
      241 tggaacatga cgaaagcgtc gttgaccgag acgcagtaga cctcatcgac gccctgtgcc
      301 ttgagttcgt cgtacttcgc ctcgtacccg ggaaggtgcg tcgaggagca cgtcggcgtg
      361 aacgctccgg gaagcccgaa gaccaccact ttcttaccct tgaagagctc gtccgtcgta
      421 acgtcctgcc agcgataggg attcggcccc tcaacggatt cgtcgcgaac gcgcgtcttt
      481 agggtcactt ccggcacgcg tgccggcatc gttggttcgt tcatagcatc gtttcgttat
      541 ggcgcgggaa gtcccgccgc ccgtcctcat aatgagtcga atttcgtgtc tgccgttacc
      601 gtaggacact attgtatcgt actcgcgaaa tgtgacaaga aaatgaagcg tgaccgttta
      661 tccacccgtg gggcccgcga ttccgtacga cgtgcataca atagggatat gacgacggcg
      721 gcattacggg agcgggtcga cggactcatg cggaagaacc gcagggtcgc cggcgagcac
      781 atgtacaccg tcccttcgcc cgaacactac ccgttccagt ggctctggga ctcctgcttc
      841 cacgcgatca tcctctctca cctcgacccg gcgctcgcgc gggccgagct ccgctccgtc
      901 ctcgcgaagc cgctcgcgag cggtctcatt ccccacatga tctactggga ggactcgtac
      961 gagggcgtga agtgggggcg cgagaagcag ggagacctcc tctccgcagc ctggggcgtc
     1021 cacggcgtga gttcgatcac ccagccgccg gtcatcgcga ccgcggtgtg gcagctccac
     1081 gtccgcgaac ccgacgccgc gttcatacgc gagatctacc cgatgctccg cgcgcactac
     1141 ctcgcgctcc tctcggagcg aagtttcgga ccgagcgggc tccttggcat catcaacccc
     1201 gacgagtccg gcgaggacaa ctcaccccgc ttcgactcgc tccagggtct tccgccgcgg
     1261 cacgacccag tcgagaactt ctcgaagcgc atcgaacgcg tccgcgagca cgtcgcgtgc
     1321 aacttcgacg tacagaactg catggtccac cgcttctggg tcgaggacgt ctcgttcaac
     1381 gccatctcca tccgcggcct cgaggcgctc tcgcgcatcg cgcgcgagat aggcgacgtg
     1441 aacgacgcgg tcctcttcag cctttccgcc cagcaacttc gcacctcgat gcaactccac
     1501 atgaagcgcg ggacgcggta ctactcgctc cagggcgagt cggaggaaga ggtcatcgtc
     1561 gatacgtgga cccttttcat gccgctcttc gccgaactcc tccaccccga ggaggcgaag
     1621 gagctcatcg agactgcgct cctcgacaag cgttcctttt tcacgaagta tcccgtcccc
     1681 tcggtctcgc ggcgcgaacc ctcgtacacc gatgatgacc tctggcgagg accgacgtgg
     1741 gccgccgcga actggttcat atacaagggt ctcctcaatt atggctacga cgacgtcgcg
     1801 agtacgatac gcgagcgcac gatcgagctt ctcgatcgct cggggttccg cgagttctac
     1861 aatgccgaga ccggcgaggg atacggcgcg cacgaattca catggggcgg cctcgttctc
     1921 gacatggacg gcgagaaact cggtgtccct acatcgcctc gagcatcata aagagcggga
     1981 actccttccg cgcgacgttc tccgccttcg cgcgaggtcc cggctcgctc gtcttgtgcg
     2041 atatccattc ctcgagccgc gtgatcgaaa accctgcgcc acggaggagt ttcatgtact
     2101 cctgaagcga gcggtggaac gagtacgtga cggatttccg tttctcgccg gggtgcatgt
     2161 cgatcgcggt acgtcgctcc gagaggtagc cgtcgagtcg gcggtactgc gtcttcgagc
     2221 cttcgtcata tccccacgat gagagcttcg gaacgcggat gacagggtga ttgaggacga
     2281 agaggaatct gccctccttc ttgaggacgc gccggacctc cttcatgacg aggtcgaggc
     2341 gctccatgtt ctggagcgta aggaccgcga ggacgacgtc aaagaacgcg tccctcgcga
     2401 aggaaaggtc ttgcgcgggg gaaacgacgt acgcgacgcc gcctccttgt ttcttcgcct
     2461 cggcgatgag ctcgggcgag atgtccgcgg cggtgaccat gcttccctcc ttagaaagcg
     2521 cgcgggcgaa gtacccctcg ccgcacccta tctcgagaac ggtctgatct ttattcggcg
     2581 cgacgagccg catgatgttc gggagaatga ccttcgcatg gtacgtatcc gcgtcagtga
     2641 ggtgctccgc gtaccacgcc gcttccttgc cccatgaggt cttagccggc tttttcatgg
     2701 cgccatagta gcacgcggca cctatttcgc gagcgaagtg aggaaggacc gaagcgtcgg
     2761 tacgtgcgaa tgttctatat ccttcgccgc cgttacgagc gtcacattct tctcgccctt
     2821 caatagttcg actgcacgat gcttttcact cttcaactct ttcgcgtact tcgttctgaa
     2881 cgattcgtag cggcgctcgg gctcctcatg gaaccactgc cgaagttccg tcgacggtga
     2941 gagttccttc gcccaaacgt cgatgtgcgc cttaaccttc gtgacgccgc gcggccagag
     3001 ccggtcgacg agcacgcgaa ggccgtcttt cttttcatac gcatcgtata ctcttttcac
     3061 ggaaatggtc atagtggtat gagctagtgg atataaacgc gtcatccgcg tacagagcgc
     3121 ttcgcccacg gaagcatcgt gacgaggacc tcgtcacgaa gaacgaagtg atggtaaagc
     3181 gccgcgccga tatgcccgag cacgaggtac ccgaggatcg tcgcgagacc gccatggagt
     3241 tccgcgagga ggcggaggta cggtgaccct gcgaagagat gcggaagctc gtacgcgccg
     3301 aagatggtga ccgtccatcc gcgccatgtc gcgtacgcga gtcccgtgag cggcacgaga
     3361 aagagaagcg cgtaaatgga atggtgaacc gccttcgcga gccgttcgtt gagcttcgaa
     3421 agtcggtacg gcgggctcgc cgggcgaaag aaacgagagg cgaagaagag cgcgacgaac
     3481 ggaaggagta tcgcgccgta ggtcatgtga atgttcacga ggtacgcggg gttcgtgctc
     3541 cgtctcaccc ctggcatgat ccacgcggtg aggaactgaa aaccgacgat gccggcgact
     3601 atccaatgga acgtgcgggc gaccgcgtag tacgctctct cgctcatgcc gcgggcttga
     3661 aaccgatagt ctgcatgatg tcgccgatga gggagtcgag gtcgaacgtc gcctcctcgt
     3721 tcgcgcactc gctcttctgc ggctcctcgt agttgccgca ctgcgggctc cgcacggtga
     3781 aggtgaggat cgcctcctta cccgcgaggt cggtcgcgta cgcgtactgg ctatacacac
     3841 ttcccgcggc acctcccatt tccgtcgtca cgcaatactc cttcccgtcg atgatacgct
     3901 tctcggttcg tccgccggac gcgtcaggag agcccgcctc ggtacacgag taggactggt
     3961 tctctatggt gaggacaggc ggccagtcgt acgcttcgac gtacgtcgta ccgaacgact
     4021 cgggataact gaaggtcgcg ttcgaggacg tcgcgtccgc cgtcttccat cccgtgggga
     4081 cgaccggcgt taccgccgtc gacgttcccg ccggactctc ggtcccgcta tcgacgctcg
     4141 gactcggcgt tctcacgccg tagatataga taccggcgat gagcgcgatc gcgatgacga
     4201 ccacgacgac gatgagcgcg tttccgcgag tgtgaggttt cataacaata gcataccgcg
     4261 gaaaaatgaa taattcgtga gcggctgtgc acgcgcgaaa aaacggcgtc gtcgcgacgc
     4321 cgcttttacg gtttctctag atcgcggcgt cgtcgcccag gttcatgtcg gccttgaagt
     4381 gctcgatgac ctcgggatgc ttcccttcct tacactccgc gacgaacgcg tccgcgctcg
     4441 cggcatcggg gaacgtcatg tacgcgagcg ctccctcgca aaccgcgttt atgtctatct
     4501 tcccggagat gggcgtaccg ccctctgacc ccgccgccgg cgtctcgacg accggcgcga
     4561 tgaggtcgag gtcggcgctc tgctggcgga ccgctccgta ccgcgcaccg atacaaaacg
     4621 cgagcgcggg aacgatgccc aagaagagga cgagcgcgcc gatgcgcgag taccaggtca
     4681 cacggttcca ttcgatcatc atgagtatga gattagtgga ttaaaatgct tgcgcctcat
     4741 tctagcacgc gcctcgaatc ggcgatcgac ttttccctat tccggccact cctgcttctt
     4801 gagaaggttc cgctcgaacg gctgtgccct tctaaggcgt gttcgcggcg gagcgattcg
     4861 cgttatcggc gcgtcgaaga tggcgccgcc catgtcccaa aggtcctcct cggcggttat
     4921 ccatttcgtg ccgaagcggc ggacgttcag cggcttcgag tagccaatga gcgtcttctt
     4981 cccgttccca tagagaaagt actcgtggaa gtacgacatg acgagctcgc gaagcgagcg
     5041 gtacaccggg tcgcggaagc gaaggaccgc atggttcgtc ttactgatgg ctccatagcg
     5101 gccattctcc gtaaagagga cgatggcgtg gtcgatgtcg gaatcgtgaa cctttaggtt
     5161 cacgatatag ggcttgcggc ccgcgagcat gaacgcggcg catgcgagga gcgcgccctc
     5221 gagacagtgt gccgtcctcg tttgaaggac gcgcttaggc gacatgcacg tctcgccgcc
     5281 tttctcgtga ttgaacggaa gcttctcgag gaacgtctgc accttctcgg gcgtgttgag
     5341 tttcttaagg aatttatatt ccgcggagtt cttcgggaac atagacttca cttcgagtag
     5401 tagtcctcgc gcgcctccgc aggctcaccg agcgaattat agtcgatcgt tatctcttcg
     5461 cccgcttcga tgtctctcgc ggcataccat ttgtagtcct cgtcaggaat ggcgttcgcc
     5521 tctcgcgagt ggttcaaata ccagccgacg gccatttgcc cgaagtcagc cggacagaga
     5581 agcttctctc ctctatccgc gcagtactcc cgaaactgtt cgggtacgtc ctccttgttt
     5641 cggacaacgc tccggttgct cacggtctca ccgtcaccga acatgcgaag gtgcgtaccc
     5701 ttcgcgatgc cgtgcgtagc gaaaacaccg acgccatgcg cagccggttt caatacgaag
     5761 gagaactcgt tcgtctcacc catgggaaaa gtatagcact cgctatacgc tccccgctgt
     5821 ggtggtcggt cgttcctctt tgtggtcctt ccgcttcgcg atgccgaaga tgtagatgat
     5881 ctcgagtatg ccaagcgtgt tcacgatgag gagtacgacg aaccacacgg gttcatggcg
     5941 acgagcggcg agccagagcg cccaaccttt ccacccgata gaccagagga cgagtatggg
     6001 caaaaatacc gctaatgcgc cgataccgag catgtccatt ccgttccacc cgttttgata
     6061 gagccaatgc atagtgagga tcgatagatt ggtaatctct caatcatgat accgcatcgg
     6121 tgaaggaggc gtgaacggct actgatactg gatgtaaatg ggtttcgggt ggagcgccat
     6181 gacctcggcg ggcgtgagca gaccggtcga cggtgtcttg aggtcgttct tatagaagag
     6241 cttcacgccg gtgaattgga ccggttcagg ggcgattacc tggccgtacg tgccgcgctt
     6301 caggtccttc gggccccagc cgtccatgtt cacgacgacc tgcacctccg gcgtcggagt
     6361 gatgcgcgac gagcccgtca ccatttcctc cgtgaagcgg tggacgatga ggaccttcgg
     6421 cggaagttca ttgtctcgta cgatcgtcga aaggtagtcg atcgcgtaat tgacgtccgt
     6481 cgcgtcgaac gtgccgatga cggtccccgg ccgctgtcct cccttcatcg agaactccgg
     6541 gtcgatggcg aggaacacgt cgggccgctc gaggtatttc ttgaacttcg gcagttccgt
     6601 ggagagcggg ctaaggccca cctggatgtc gagtatgagg acaccgtgta tctccttcgc
     6661 cagttcgtac gctttatcta tctctttatc gggcatgacc gcgcggtaca tgccgtccgc
     6721 acctgcgctc ccctgtgcga cgatggcgat gtactcgatc gcgggaagga ccggcgtcgt
     6781 cgggtcttcc ttctcccagt ctgcgacctt ctctgcgagg tgcgcgagca tgtccgtcgg
     6841 ttcgagttcg ccgagtatac ccatctgcct cgagtagaaa tttccgtagt acgcgacgat
     6901 gcgcttgaac ggaagtatcg catcgccatt ggggtacggc gcggcggtcg gccagagact
     6961 ccccttcacg gtgacgttcg tcgtcgaggc gtagcgaagc gg
//
LOCUS       DASTSP010000040         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_534749, whole genome shotgun sequence.
ACCESSION   DASTSP010000040 DASTSP010000000
VERSION     DASTSP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534749"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1896)
                     /locus_tag="VFS75_01750"
     CDS             complement(298..1896)
                     /locus_tag="VFS75_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4677425.1"
                     /translation="MYFSHAPLKILGIAALAIVLIALALGTYDYKEAQYGQSKWTEII
                     ARTIGGEVNAFGYSLRSLVGLSGVSYERNRDGSETARAIPVLTYHAIVSDNDKENVSP
                     ESFEGHNVGQREFEDQMFTLKRNGWHTVTLEEFDAFMREGKSLPAKSFLLTFDDGAKE
                     SYYPVDPLLKVLGYSAVSFILPAYSSGEGTHYYLSKAEIGRALASGRWEIGSHGEDIH
                     RFVQTDDASSSAPALANRAWLPAEGRLETVDEYETRVKDDLVTAKEQLSNIFGRPITA
                     FAFPFGDYGQLTSNVPNASDFLKAASAGVYDYTFYQWYKGEGYTYNYPDMRGSMVKRI
                     SVHPDWSGAELLALLENGLPKNLPYEDDFSSDQGWLDSWGTHEVKDGTLTLRAVGGEA
                     GASAILDGTGDWRDYDAHITVASAKNTGVFVFVRFKGNDDYADCNIGNGFAHVEQMVG
                     GDFRVIKGNRSGAIVIPEGPFTVDVHVRGREVTCGINGTTLVDSTFLDESLDRGGIGF
                     KAWDKVPNIATLVLDTLSVSRSTE"
     gene            complement(1913..>2108)
                     /locus_tag="VFS75_01755"
     CDS             complement(1913..>2108)
                     /locus_tag="VFS75_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4677426.1"
                     /translation="SQLPLVYLSGLFLMLLLHGIYYRIQVGKKAWVLAIITFWFNTVL
                     LMWQLPWAILTIKDSRWGTR"
BASE COUNT          412 a          634 c          609 g          453 t
ORIGIN      
        1 ggtgaggaca ccgcacgtgc cggagtcgcg gcaaagatgg acgccgcctt gaactcggac
       61 ggtacgcgcg acgcgagcca agcgctcgtc gacgcgaagt acatgacggt cagtaagcct
      121 atgactatgg cactcgtgac gctcataaat aataaacctc tgtgtacgta ctgctgtatt
      181 gtttttgtca tcggggcaca tcgtatcagt gcccgaagca cccggtcaag aaaatatggc
      241 ttaactacgt cataaaatct tcactgtggc ttctctcggc tctctttttc gtacggatta
      301 ttcagtagac cgcgaaaccg atagtgtgtc aagaacgagc gtcgcgatgt tcgggacctt
      361 atcccacgct ttgaacccga taccgcctcg atcgagcgat tcatcgagga aggtgctgtc
      421 gacgagcgtc gtcccgttta tgccgcacgt cacttccctg cctcgcacgt gcacgtcgac
      481 ggtgaacggc ccctccggga tcacgatcgc ccccgagcga ttacctttga tgacgcgaaa
      541 gtcgccgccg accatttgtt cgacgtgtgc gaagccgttg ccgatgttgc agtccgcata
      601 gtcgtcgttc cctttgaagc ggacgaacac gaacacgccg gtgttcttcg ccgaggcgac
      661 ggtgatgtgc gcatcataat ctcgccaatc gcccgtgcca tcgagtatcg ccgacgcgcc
      721 ggcctcgccg ccgaccgccc gaagcgtgag cgtgccatcc ttgacctcgt gcgtacccca
      781 cgagtcgagc cagccttggt cgcttgagaa atcgtcctca tacgggaggt tcttcgggag
      841 tccgttttcg aggagcgcga gcagctccgc gccgctccag tccggatgga cgcttatgcg
      901 cttcaccatc gacccgcgca tgtccgggta gttgtacgtg taaccctcac ctttgtacca
      961 ctgatagaac gtgtagtcgt agacgccggc agaggcggcc ttcagaaaat ccgatgcgtt
     1021 cgggacgttg ctcgtaagct ggccgtaatc accgaacggg aacgcgaagg cggtgatagg
     1081 tcgcccgaag atgttcgaga gctgctcttt cgccgtcacc aggtcgtcct tcacccgcgt
     1141 ctcgtattca tcgaccgtct cgagtcgacc ctccgcggga agccacgcgc gattcgcgag
     1201 cgcgggtgcg ctcgacgatg cgtcgtcggt ctgtacgaag cggtggatat cctcgccgtg
     1261 cgagcctatc tcccaccgac cgctcgcaag cgctcgtcct atctccgcct tcgagagata
     1321 gtagtgcgta ccctctccgc tcgagtacgc cggcagaatg aacgaaacgg cgctataccc
     1381 gagcactttg aggagcgggt cgaccgggta gtagctctcc ttcgctccgt cgtcgaaggt
     1441 gagaagaaac gacttcgcgg gaagcgactt cccctcgcgc atgaacgcgt cgaactcctc
     1501 gagcgtcacc gtatgccatc cgtttcgttt cagagtgaac atttggtctt cgaactctcg
     1561 ctgtccgacg ttatgcccct cgaacgactc agggctcacg ttctccttgt cgttatcgct
     1621 cacgatcgcg tggtacgtga ggaccggaat ggcgcgagcg gtctcactgc cgtcgcgatt
     1681 gcgctcgtac gagacgccgc ttaggccgac gaggctccga agcgagtagc cgaacgcgtt
     1741 cacctcgcca cctatggtgc gggcgattat ctccgtccat ttcgactggc catactgcgc
     1801 ctctttgtag tcgtacgtcc ctagtgcgag cgcgataagg acgatcgcga gtgccgcgat
     1861 gccgagtatc ttcagtggtg cgtgcgagaa gtacatatat ctatcggtac gattatcgcg
     1921 tcccccatcg ggagtcttta atggtgagta tcgcccacgg cagctgccac atgaggagga
     1981 ccgtgttgaa ccagaacgtg attatggcga gcacccacgc ctttttgccg acctggatgc
     2041 gatagtagat gccatggaga aggagcatga ggaagaggcc ggagaggtag acgagcggca
     2101 gctggctt
//
LOCUS       DASTSP010000041         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_756808, whole genome shotgun sequence.
ACCESSION   DASTSP010000041 DASTSP010000000
VERSION     DASTSP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756808"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFS75_01760"
     CDS             <1..851
                     /locus_tag="VFS75_01760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU4677427.1"
                     /translation="PVRYRVGNIDPRFDANAEEVKRVAAAAEAIWEGKLGRELFVYDE
                     RRGIPINLVFDERQENAEIEENLRDDLEAKAGMNDAVAKQYDALIGEFRSLKKAYESR
                     VRSYETKLAAYNDTVNDWNAKGGAPESEIERLEADAASLKAEQEGLEADAEKLNALVD
                     QLNAIGAKGNTLITDYNSVVAEYNDKVAEAGEFTQGDYTGDAINVYQFNSEDELELVL
                     AHELGHALSLDHVDGKRSIMYRLMEDQAVDDGLTPEDIAEFDAVCAQKSAPAEFLAAL
                     REKMTF"
     gene            910..1695
                     /locus_tag="VFS75_01765"
     CDS             910..1695
                     /locus_tag="VFS75_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU4677428.1"
                     /translation="MFQELYMRRILATLGIVGIVALVAYSYQAFKQAEYMYHGPTVIS
                     VSGKGEVFAKPDVATFSFTVSTKEADAVTAQNKAADTMNGITAYLKDAGVDEKDIKTT
                     SYNLSPRYEYSSQVCTVTYCPPAKEPKQIGFAVDQTVSVKVRDLDKAGEIVSQVGGKG
                     ATNVSGLSFTIDDPESLRAEARAKAIDDAKAKGKVLANELGTHIVRMSGYWEDNGGPI
                     IYGMGKGGAVESAAVDQVAPRPAELPTGENTITVNVTLNYEIR"
     gene            1819..2001
                     /gene="secE"
                     /locus_tag="VFS75_01770"
     CDS             1819..2001
                     /gene="secE"
                     /locus_tag="VFS75_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU4677429.1"
                     /translation="MSHFINYLKDTVAELRHVTWPTNRQSVVYTALVIAISAVVAFYI
                     GFFDFVFERAVNWLIR"
     gene            2101..2646
                     /gene="nusG"
                     /locus_tag="VFS75_01775"
     CDS             2101..2646
                     /gene="nusG"
                     /locus_tag="VFS75_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU4677430.1"
                     /translation="MAKQQANGERHWYAIHTYSGYENAVARNLKQRIESLGMQDRIFD
                     VVVPTEKKIRVKGGKRVTEEERIYPGYVLVEMIVTDDSWYVVRNTPRVTGFVGSGTQP
                     VPLRDDELKALMTRMQDDAPKHHVDLSIDDLVMIVDGPFKELEGKVGEIDEGRGKVKV
                     LVSMFGRETPVELDFLQVKKM"
     gene            2864..3289
                     /gene="rplK"
                     /locus_tag="VFS75_01780"
     CDS             2864..3289
                     /gene="rplK"
                     /locus_tag="VFS75_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU4677431.1"
                     /translation="MAKKVVKKIKVQAVGGKATPAPPLGPVLGQAGINIGEFVNQFNE
                     KTRERMGDVVPVEINVYDDRSFDFIVKTSPASRLVLKKMGIEKASGKNVASPTGTIKR
                     ALVREIAEEKMQDLSANDVEAAMKIIEGTCRSMGVKVID"
BASE COUNT          818 a          983 c         1095 g          640 t
ORIGIN      
        1 tacccgtgcg ctaccgagtc gggaacatcg acccgcgttt cgacgcgaac gccgaagagg
       61 tgaagcgcgt cgccgccgcg gcggaggcca tttgggaggg gaagcttggt cgggaactct
      121 tcgtgtacga cgagagacga ggcatcccca tcaacctcgt tttcgacgag cggcaggaga
      181 acgcggagat agaggagaac ctacgcgatg acctcgaggc gaaggcaggc atgaacgacg
      241 ccgtcgcgaa gcagtacgat gcgctcatcg gtgagttccg ttccctcaag aaggcgtacg
      301 agtcgcgcgt gcgctcctat gagacgaagc ttgccgcgta caatgacacg gtgaacgatt
      361 ggaacgcgaa ggggggagcc ccggaatccg agatagaacg gctcgaggcg gatgcggcgt
      421 cccttaaagc cgaacaggaa gggctcgagg cggacgcgga gaagcttaat gcgctcgtcg
      481 accaactcaa tgcgatcggg gcgaagggga acacgctcat caccgactac aactccgtcg
      541 tcgccgaata taacgacaag gtggccgagg ccggggagtt cacgcaggga gactacacgg
      601 gtgacgccat caacgtctac cagttcaatt ccgaagacga gctcgagctc gttctcgccc
      661 acgagctcgg ccacgcgctc tcgctcgacc acgtcgatgg gaagcgctcc atcatgtatc
      721 gcctcatgga ggaccaagcc gttgacgatg gcctcacgcc cgaggatatc gccgagttcg
      781 atgccgtgtg cgcgcagaag agcgcgccgg cggaattcct cgccgcgctt cgcgaaaaaa
      841 tgacgttttg agaaaccgcg acggcgcggt aggatactcg tgtaatcgct aattcacgtt
      901 ttcatcacta tgttccaaga gctttatatg cgccgcatcc tcgccacgct cggcatcgtg
      961 ggcatcgtcg cgctcgtcgc ctactcctac caggcgttca aacaggccga atacatgtac
     1021 cacggtccga cggtgatctc cgtttcgggg aagggcgagg tcttcgcgaa gccggacgtc
     1081 gcgacgttct cgttcacggt gagcacgaag gaagccgacg ccgtgaccgc ccagaataag
     1141 gcggccgata cgatgaacgg catcaccgcg tacctcaagg acgccggcgt cgacgagaag
     1201 gacatcaaga cgacctcgta caacctttcg ccgcgctacg agtactcgtc gcaggtctgt
     1261 acggtgacgt actgtcctcc ggcgaaggag ccgaagcaga tcggcttcgc cgtcgaccag
     1321 acggtgagcg tgaaggtgcg ggatctcgat aaggcggggg agatcgtctc gcaggtcggc
     1381 ggtaagggcg cgacgaacgt gagcggactc tcgttcacga tagacgaccc ggaatcgctc
     1441 cgtgccgagg ctcgcgcgaa ggccatcgac gacgcgaagg cgaagggaaa ggtgctcgcg
     1501 aacgagctcg ggacccacat cgttcgtatg agcgggtatt gggaggataa cggcggtcct
     1561 atcatctacg gcatggggaa gggtggcgcc gtcgaatcgg ccgcggtcga ccaggtggct
     1621 ccgagaccgg cggaacttcc gacgggagag aacaccatta cggtgaacgt gacactgaac
     1681 tacgagatcc ggtagaaccg aaaggaagga aaaccgaggc ggaaagcctc ggtttttcgt
     1741 tgcattgaca acgcctcgga gcgtagtact atggtcgtga tcgcccccgg ggcgttttaa
     1801 ttttgctaac cacataccat gagccacttt atcaactatc ttaaggacac cgtcgcggaa
     1861 cttcggcacg tcacgtggcc gacgaaccgc cagtcggtcg tctacacggc gctcgtcata
     1921 gccatctcgg cggtcgtcgc gttctacatc gggttcttcg atttcgtgtt cgagcgcgcg
     1981 gtcaattggc tcattaggta agtgagacag agttcgttcg tcatttcgag gcgctatggg
     2041 gcgcacgaaa tcacggccga cttttcctaa attcattaca ttcattaaga aaaactaggc
     2101 atggcaaaac agcaagcgaa cggcgaacgg cactggtacg cgatccacac gtattcggga
     2161 tacgagaacg ccgtggcgcg taacctcaaa cagcgtatcg agtccctcgg catgcaggac
     2221 cgcatcttcg acgtggtcgt cccgaccgag aagaagatac gcgtgaaggg agggaagcgc
     2281 gtgaccgagg aggagcgtat ctacccggga tacgttctcg tcgagatgat cgtgaccgac
     2341 gactcgtggt acgtcgtccg caacacaccg cgagtgacgg gtttcgtcgg aagcggcacc
     2401 cagccggtcc cgcttcgcga cgacgaactg aaggcgctca tgacgcgaat gcaggatgac
     2461 gcgccgaagc accacgtcga cctctcgatc gacgacctcg tcatgatcgt cgacggaccg
     2521 ttcaaggagc tcgaaggcaa ggtcggcgag atcgacgagg ggcgcggaaa ggtgaaggtg
     2581 ctcgtctcga tgttcgggcg tgagacgccg gtcgagcttg atttccttca agtgaagaaa
     2641 atgtaatcgt cccggagatc cccgactgct ttgtgggtcg ctcggatgtt caccgtatcg
     2701 ttatacgact cacatcctcg cacggcggcg cctcgcattc gagcaatctc cgaaacgatt
     2761 ccgaaaccat tgcacagtcg agatttttct tgtacgatac gcgggcttcc agtgaggacc
     2821 cggctatgga acgggaccta caaacagtaa tcaaatcaat accgtggcaa agaaagtagt
     2881 taagaagata aaggtccagg ccgtcggcgg caaggctacg cccgcgccgc ccctcggtcc
     2941 cgtgctcggc caggccggca tcaacatcgg cgagttcgtc aatcagttca atgagaagac
     3001 gcgcgagcga atgggtgatg tcgtccccgt tgagatcaac gtctacgacg atcgaagctt
     3061 tgacttcatc gtgaagacga gccccgcatc gcgcctcgtc cttaagaaga tgggcatcga
     3121 gaaggcgtcg gggaagaacg tcgcgtctcc gaccggcacc atcaagcgag cgctcgtccg
     3181 cgagatcgcc gaggagaaga tgcaggacct ctcggcgaac gacgtcgagg ccgcgatgaa
     3241 gatcatcgaa ggcacctgcc gcagcatggg cgtgaaggtg atcgactaga gaaccttcac
     3301 ggaccttgag cagttgctgc gaaaggtata cggcttctgt aagaagggaa ggtgatcgac
     3361 tagagaactt ttacggggtt ctcgacaaaa ccggccctta ggccggtttt atcgagtctg
     3421 tgtaccagag ccgttttcga aatatcgtgg tcgtgctata gtctcgcgca gaaaacgagt
     3481 tgtcttgtct aacctgcgaa ggagacggct ttgtacgata acaagggaaa cggaag
//
LOCUS       DASTSP010000042        43795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_536221, whole genome shotgun sequence.
ACCESSION   DASTSP010000042 DASTSP010000000
VERSION     DASTSP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 43795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43795
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536221"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1761
                     /gene="argS"
                     /locus_tag="VFS75_01785"
     CDS             79..1761
                     /gene="argS"
                     /locus_tag="VFS75_01785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU4677432.1"
                     /translation="MRMEGEIRELIDAALAAKGLDAVAVTVEHPADESHGDYATNAAL
                     VAAKRLGANPRALAEDLVAQMAGKSPNIESIEVAGPGFINFHIARSFFAEAVANARAK
                     GDAYGTNDTRKGQEILFEYTSPNLWKPLHVGNLVGNIVGESVSRLFEAEGATLRRINY
                     PSDIGLTVAKGVWGLKKTGGDPEDINAIGEAYRVGNEAYETDETAKTEIEAVNRALYA
                     GDDEGLKALRERGLRTSHRHLMELCRMLGTSFDLEIRESEVSDIGTELVRTHTGDVFV
                     ESNGAVVFPGEQYGLHTRVFLNSQGLPTYEAKDLGNFARKERAYPHWTHSYIVTGGEQ
                     REYFMVLFEAMRRVFPDTADKILAHIPTGFLTLTTGKMSSRKGNVLTAESLLASLMEE
                     VEKRATESRAENLASLVEEVAVGALKYQILRQAAGQDIVFDKERALSLEGDSGPYLMY
                     AHARMSSIEEKAAAAGMHAGTDAPEVPYALERFLYRFPEVVLRAGHECAPHHLVVYLT
                     ELASRFNAFYAVEKIVDDGDPYAPYKLSLTKAARVVLRNGLALLGIAAPERM"
     gene            2064..2600
                     /locus_tag="VFS75_01790"
     CDS             2064..2600
                     /locus_tag="VFS75_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677433.1"
                     /translation="MKQVVLGLFSSRARAEELIRHLTESLGVRTDDISYIYRNTEGDV
                     EAAPATDAHTPASGAATGALVGGSVGALAGIATAIGVIPVIGPIFAAGPLVAALGIGT
                     ASAVGTTAVGAATGAVAGGVIGALVNLGAPDVEAKEYENRILAGDVLVAVTTDEVPPV
                     HTAFTTYGATDLRTYGAK"
     gene            2700..6197
                     /locus_tag="VFS75_01795"
     CDS             2700..6197
                     /locus_tag="VFS75_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4677434.1"
                     /translation="MENNEKSDAARREEEVLAFWKERDIFHKSLTKDAPKGDYVFYDG
                     PPFATGTPHYGHILAGTIKDAIPRFFTMRGYRVKRKWGWDCHGLPLENIIEKKLGLAT
                     KRDIEELGVKKFNEAAREAVLEYEDDWKEVVPRMGRWVDMEHDYKTMDSTYTESVWWA
                     FSELDRQGLVYEGFKSMHLCPRCGTTLSNFEVNQGYKDIKDIAVTVKLPLLGTDGNVT
                     DTSLLVWTTTPWTLPGNMAAAVNSEIEYVKVRVPIEDGDEVVILAKERLAQLDTDAYE
                     VLETMKGKDLVGLRYEPPFDYYKHEEFQNKEHAWKVYHADYVEVGEEGTGAVHLAPAY
                     GEEDLELAKREGIPVIHHVGEDGRFKDVITDFKGRLAKPKDDDATGVTHLEADIEVVK
                     ALKERGRLFRKENITHSYPHCWRCDTPLLNFATTSWFVRVSEMRDTLVAENAKIRWVP
                     EYVGRARFGNWLEGARDWAISRQRYWGAPLPIWRNPKTKEHRVIGSLAELQARVRKSG
                     NTYVIARHGESVFNTKSLLSSLAETENPLSATGREQVKHAAKALRERGIDVIYTSPLG
                     RTRETADMFAEALGMPRERVIEDERLGEVRFGEFEGKLIDEYHAYFKDGHERLTKRPE
                     GGESWTELKRRMGSFLYDIDAKHTGERVLIVSHNGPIQMLRAAAAGDDRATTAKRIDD
                     NAYDVANAELSDLAFTPMPHNDDFELDYHRPYIDEIDLFDPDGRKLERVRDVFDCWFE
                     SGSMPYGQNHYPFENLDAFDPGKGAGYPAEFIAEGLDQTRGWFYSLLVLGTALFKRAP
                     YRHVIVNGLVLAEDGKKMSKRLQNYPDPMALADRVGVDAMRFYLLSSPVVRGEDLNFS
                     EKEVRELERKNIGRLHNVLAMYEMYADGTAASDSSDNVLDRWMVARLREATKEMTDGY
                     LAYELDRATRPIEGLIDDLSVWYLRRSRERLKGIDAKDRARTLSTLRYALRTVALLMA
                     PAMPFYAEYLFGRVREAGDRESVHLMDWPASVEPDGHIIADMAEARRVTSLGLEARSK
                     ANIKVRQPLALLRARANARVEYLADIVKAELNVKRLEITPDLPVDVELDTEITSELRR
                     EGDARELIRAVQDARKEEGLDPRDRVTLYVDGRTRDLLMGFEKELSHVVGAEEIAVGE
                     TTKEVKTEGEPLRFSIAKR"
     gene            6197..6772
                     /locus_tag="VFS75_01800"
     CDS             6197..6772
                     /locus_tag="VFS75_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677435.1"
                     /translation="MAYKTYITNALVCAGFDSHSADRSFLLFTREAGMLFASAKGVRK
                     EISKHRYALQECSEARVTLVRGKAGWRVTGAEPVRNFYAMARTREARALLKNAILLIR
                     RVMHGETPHAGVFDDVIASFERMHDHRYETLGSVLSLRVLHALGYIAPSPEYDLFLSP
                     TFPFSAVGDLKDETAEALERAVERALVESHL"
     gene            6802..8796
                     /locus_tag="VFS75_01805"
     CDS             6802..8796
                     /locus_tag="VFS75_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677436.1"
                     /translation="MSNEEARREQPEPSDPRRVPEDRIKELRERLYARGDAPTDVRHK
                     LPERPHEPLRSDIQPARPAPEPETERAEKPREEQTVPYTRPMAANSMRSSFRKKFLIF
                     GFLFFVIAIGLSAAFMFLGTHSVSGENISVNVTGPLTVNGGGEYPFQVSIANQNTVPI
                     QSATLIVEYPAGTHSATDPNAEVSIIRQPLDTIGSGEIVNIPLRARIFGEENDEKEIK
                     VSVEYRVEGSNATFEKEAAPLSFKVGTSPVVVTIDGLKSISSGQELDFTITVQSNSPA
                     PLSNLLLKATYPDGFDFTSSKPETLAGEDTWGIDTLKPGEKKVIKVTGLMTGYEDETR
                     SITVEAGVPAGADISHIASMLASATADVKIEKSFLGVDLSVNGMETEPVIMNATEVAN
                     ARVTLTNSLDTAIYDGKVTIELTGNALDEFNVKSAGGFFDSSKNTITWDSAEFENLKE
                     ILPGEDVILGFTLEPKQNVGTAPEIHLKVTAEGSRVFEDRAPEKLSGIAERTVKIESI
                     SSVDAAAYHATGPFTNTGPIPPVAEEVTQYTLTFRVKTGANDVTGAEVTAALPQYVSW
                     LDLVSEGADVSYNPTTRTVKWTLGKVDANTTKETSMQVSFKPSLSQVGTMPTILEAQR
                     FKATDRFTGTVVRAEHPAVTTSLTNEPDSAAEDGRVRASE"
     gene            8859..10172
                     /locus_tag="VFS75_01810"
     CDS             8859..10172
                     /locus_tag="VFS75_01810"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009832726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU4677437.1"
                     /translation="MEHSAEKMEKIVSLCKRRGFVFQGSEIYGGFAGAYDYGPLGVAL
                     NNNLKREWWKMFVESREDMYGLDAAILMNEKVWEASGHTGAGFSDPLVECEKCKRRFR
                     ADHLENKETCPECGGKLGEERQFNLMFETKVGAQGDAKSYLRPETAQGIFVNFKNVLD
                     SMSPKLPFGIAQIGKAFRNEIAPREFLFRVREFEQAEIEYFLKPEDWERLFEAWREDV
                     WKWIDHIGVAREHVRELEVDANDLAHYSKRTVDFEYDYPGKGFAELLGLAYRTDHDLR
                     SHMEASGEKLEYIEPDGTRFIPHVIEPSFGLGRAFIAVLAEAYTEDEMNGEVRTYLKL
                     PTHLAPYRAAVSPLLKNKPELVTKAREVFSMLKQEFGNVAWDDNGNIGKRYRRQDEIG
                     TPACIVIDFETIEGETKDTVTVRDRDTGAQVRVPIADLVSHLTSA"
     gene            10181..11599
                     /locus_tag="VFS75_01815"
     CDS             10181..11599
                     /locus_tag="VFS75_01815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU4677438.1"
                     /translation="MANKYLTTTLPYVNADPHVGFAYELVAADALVRYWKATGDDVFF
                     NTGTDEHGQKIAGKADEKGESRQAYVDHYANEFRKLIPALGVSEDIHFIRTTDERHKK
                     AAEELWRRCEAAGDIYKKKYKGLYCVGDEMFVKESELVDGRCANHPNLELEEIEEENY
                     FFKLSNYQSYLEEYLSRDGVIVPEWRRQEARSFVKKGLEDFSISREKARLDWGIPVPG
                     DENQVMYVWFDALTNYVSTLGWPEDTEGNYEKFWKSGEPMQIAGKDQVRFQSVMWQAM
                     LKSAGLAPTAKVLYHGFITSGGQKMSKSLGNVVSPFELVSRYGTEATRYLLLRHVHPF
                     EDTDVTWERLDEWYNAGLVNGIGNLTARILKMSETHLGAFEAHDFISEMKGMEEYDCK
                     SALDYLWSRIQALDEKIAETEPFKLVKTEPERAKAILEELRADLYRLSIELRPFMPAT
                     AVTIEEAVKANKKPENLFARID"
     gene            11601..11966
                     /locus_tag="VFS75_01820"
     CDS             11601..11966
                     /locus_tag="VFS75_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU4677439.1"
                     /translation="MRKVYNKLVRDKMIDVYKHDVENKISSSDYSVRYLNPAETLEEL
                     KNKLLEEVAEVFEAYGKDREHLKEEIADVIEVIDAILFHNGMTLDEVLTIRDAKKAKK
                     GGFETGLYLESIDYFDSAE"
     gene            12473..13087
                     /locus_tag="VFS75_01825"
     CDS             12473..13087
                     /locus_tag="VFS75_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4677440.1"
                     /translation="MTENGKGIVDIGRLILAFAKVNRATTMEDGVTPESDTDHTVMLS
                     VVASAFAKKLYPDSLDVGRVAEFAIVHDLVEAYACDTDSFGISAEAKRAKDEREREAY
                     LRIEREFRDTFPWIPETIAAYERLDSREARFVKTADKLLSKVTHILNGGAYFKSRGMD
                     EETMWNNYQQMVRAAEEKYGAEFPEVLALMDELIREAKRIAYGA"
     gene            13077..13475
                     /locus_tag="VFS75_01830"
     CDS             13077..13475
                     /locus_tag="VFS75_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEU4677441.1"
                     /translation="MEHKTFKDIYAIAERNVKENPYVREKEADEIIGIYLDWLRGEVE
                     EVRDETKPRNRIHLADELADILWVYAGLLAVLKDRGYIDSVEEVFMQSHEKYTERMPA
                     FLRSSAELWESIKARQKEDLALRHQKEYGQ"
     gene            13465..14277
                     /locus_tag="VFS75_01835"
     CDS             13465..14277
                     /locus_tag="VFS75_01835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU4677442.1"
                     /translation="MDSEFRYIDVHTHLNLDAFDADRTAVETRTLEDRVAHINVGTAR
                     ETSEAAIRLAESFNAGVYATVGLHPVRASGASEHEGTEAFDHEAFQALGAHEKVVAIG
                     ECGLDYFRVEKDTKAKQEGAFSAQIALANELEKPLMLHIRDEKGKMDAYEDALAILKR
                     EAKVLGNVHFFAGTYDIAKRFWEMGYTTSFTGVVTFADEYDDIIKNAPLDMLHAETDA
                     PYVAPVPFRGTRNEPAHVREVYARIAALRSEDPEVVRRALLENARRLFGVLI"
     gene            14331..14621
                     /locus_tag="VFS75_01840"
     CDS             14331..14621
                     /locus_tag="VFS75_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677443.1"
                     /translation="MEPGEETCGVLTSDIHITGGSAEPLECLRPRHQFGQHLIRRDDG
                     GYVAYETDWTCGCEMCQSDEPDDWCEIYRDVSDEEAKYLLGSADAELKGFQD"
     gene            14695..15498
                     /locus_tag="VFS75_01845"
     CDS             14695..15498
                     /locus_tag="VFS75_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677444.1"
                     /translation="MRRVYDLSFVSKDVSNSGAPEIIISIQPEHVTQFKVGIGEYATR
                     YSENAERYLGEMPNFVSPTNCRFGYRGCGEVREGRTLDIAFPLYDFDGRHVAYTLMVT
                     FEELRTFFPATRTAELPSEKSQYVDVSLPCRQAVHGHNPSGWILPHFGMWLRAKGGEP
                     REQGTVRRKVTQAIFEVWNALVHRRQKQGTQRAVLLIGDNGHFVLHCPGDACDLGVYP
                     DLVREGMGTRPVRFESHNVESYVQGLALLSGLAALATIAREENEHVHQH"
     gene            complement(15538..16473)
                     /locus_tag="VFS75_01850"
     CDS             complement(15538..16473)
                     /locus_tag="VFS75_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU4677445.1"
                     /translation="MVDLSERKLRIWLISGAALIVVLFAVSGYELYVIRTLESENANL
                     GNDLNAVRTTLSEREATFADDEATLEALKAAYATSEATGADLLKQLSEEKNRNEDFED
                     QIRSITKDVTKLDKLSKLDPELLKKYSKVYFLNENYKPAKLEEIPEEDRLRKNEPEYI
                     ESEVLPFLEDMLSDAKEDGVDLLVLSAYRSYEEQKGLKSAYTVQYGSGANTFSADQGY
                     SEHQLGTTVDFTTAALGGLTGFENTAAYTWLQKHAYRYGFVISYPENNGYYVYEPWHW
                     RFVGTDLAHDLHVDDKYFYDIEQRTIDTYLLHIFD"
     gene            16607..17170
                     /locus_tag="VFS75_01855"
     CDS             16607..17170
                     /locus_tag="VFS75_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU4677446.1"
                     /translation="MTESTMPAAEAAAQSYDERNDYEFAFHVLPTATEGEVTSAFEAI
                     KAEIAKEGEITGEEAPERIELAYPIVKSIEGKNRKFASAYFGWVRFKLEAEKLEAVKE
                     ELASVNVILRHLLIKLTREEVAEPFRFHENRKSVKMVEVVNEEPQALPEAPRETEARA
                     EVSAAALDESLEKITKDEGEKKEESAS"
     gene            17193..17621
                     /gene="ssb"
                     /locus_tag="VFS75_01860"
     CDS             17193..17621
                     /gene="ssb"
                     /locus_tag="VFS75_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4677447.1"
                     /translation="MYLNKAMVYGNLTRDPELKSLPSGMQVCSFGIATNRVYKDRDGK
                     RAEQTDYHNIVVFGRQAETCAQYLKKGAGAFVEGRLQTRSWEQDGAKQYRTEIIADRV
                     QFGPKSTGGGAPMGASPKAEPAGNAPEYPEEEINPEDIPF"
     gene            17628..17831
                     /gene="rpsR"
                     /locus_tag="VFS75_01865"
     CDS             17628..17831
                     /gene="rpsR"
                     /locus_tag="VFS75_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU4677448.1"
                     /translation="MEKEKKQKTTNVEHYDYKDVQRLRAHMNPHARMMSRKRTGHNAH
                     EQRVFATAVKRARFMALLPYVSE"
     gene            complement(18114..18689)
                     /locus_tag="VFS75_01870"
     CDS             complement(18114..18689)
                     /locus_tag="VFS75_01870"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU4677449.1"
                     /translation="MSVLSYNEITLKKVILWNDEPHIVLASHVFRKQQRKPVNITKLK
                     SLISGRVIEQTFHQNETAEEADIESRPIEFIYENRGEYWFNTPGKPAERFALSGDIVG
                     DEIKFLKPKTVIDAVVFDEKIIGVKMPIKVELKVKEAAPAVRGNTSSGALKEAILETG
                     AKIMVPLFINEGDVIRINTESAEYTERVEKA"
     gene            18739..19329
                     /locus_tag="VFS75_01875"
     CDS             18739..19329
                     /locus_tag="VFS75_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4677450.1"
                     /translation="MFGLSTGDSARDLTDEAILSRVQQEPWLFTALLERYQAAFLRKA
                     KTILHKNEDAEEVVQDAFTKIYMNAHRFEVRPRAKFSSWAYAILINTALTRYQKCVRE
                     GKRTMLIDPEYMEQLGEMKEHNAFRQDKDAIDRLLEKLPGHFARVLRLHYLEHWSHKD
                     IAAETGESVGAVKARIHRAKAEFRKQAGAEGASLLG"
     gene            19370..19690
                     /locus_tag="VFS75_01880"
     CDS             19370..19690
                     /locus_tag="VFS75_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677451.1"
                     /translation="MKKLQRKIMRGIYYAYALRLVSLPGVLQGFFMLAIMIALTRFVS
                     LGHVIANFETGGFAHVGTFLYNAVRTTEIWTLLLIGLFVFSLLSLRISLVPRREEAYS
                     FARI"
     gene            complement(19743..20753)
                     /gene="recA"
                     /locus_tag="VFS75_01885"
     CDS             complement(19743..20753)
                     /gene="recA"
                     /locus_tag="VFS75_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU4677452.1"
                     /translation="MGDIAETLRSIKTKFGDDAIMTLGSTRKVDIDAIPSGSIGLDDA
                     LGIGGYPRGRIIEIFGPESSGKTTLSLHAIAEAQKQGGICAFIDAEHAMDPEYAARLG
                     VKLDELLISQPESGEQALEIVESLVRSGKIDIVVIDSVAALTPKDEIEGEMGATHVGK
                     QARLMSQALRKLTAITAKSKTIVIFINQIRMQIGVMFGNPETTPGGKALKFYTSVRLD
                     IRRIAQIKKGDEVVGGRHRVKVVKNKVAPPFRVTEFDLIYNEGISREGELLALGEKYG
                     LVKKSGAAYSYLPPGGDQSAEVKLGRGYDAARTTLREDKKLQNELLKHVRKALKDEGT
                     AA"
     gene            complement(20887..21255)
                     /locus_tag="VFS75_01890"
     CDS             complement(20887..21255)
                     /locus_tag="VFS75_01890"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677453.1"
                     /translation="MRVILSNITLGILLRFLGVSLLVIGIVGYVEFQARNLIHGPSIT
                     LTEEPPLVERDRTVTLRGEAENIVKLTLNGREIHTNEAGEFAQDLILENGLTIVTLAA
                     KDRFGRTTTLTERYVYDPES"
     gene            complement(21260..23419)
                     /locus_tag="VFS75_01895"
     CDS             complement(21260..23419)
                     /locus_tag="VFS75_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU4677454.1"
                     /translation="MAKRRRPTTPPPAGPSFFHDVSPQTKHAIAAIFFVVLAVFFVLS
                     SLGYAGIAGDMTFRALTYLFGWGFVLAPVACLLFVYVFMKPLEDNPVSVSKLVGVTLF
                     FLASLGLMELWFTEGGGAGALMLYPFASLFGGIVAGIMLVAILLIALFLTFNTSFMDF
                     LHRKRDEEVDEALEPVVTMPLPDSGEPLEGAAAAAEANADDEKPKKRSLTEKFLSKAA
                     GEFVVSSFEGTYAPPPLSLLHKDKGTPDVGDVKASSNTIRRTLKKFNIDVEMDSVTIG
                     SSITRYAIKPAEGVKLEKIVGLQTNLEYALAASPIRIEAPIPGKSLVGIEVPNRQKAM
                     VGLASLFSSPEYTDSPKPLLVALGKNITGEAWFADIARMPHGLIAGTTGSGKSVAIHN
                     VVISLLFRNSPDQLRFIMVDPKRIELTLYNGIPHLLTPVITDAKKVIVALRWAIKEME
                     RRLDLLSEAKVQNIASYHDHVYHPAKEKFEKKGSPEEERGELPEPLPYIVVIIDELAD
                     LMTAYPKELEACIVRLAQMSRAVGIHLILSTQRPSVNVITGLIKANVPTRIALQVASQ
                     IDSRTILDQSGAEKLLGRGDMLFLSAESPKPTRLQSAFVTTEEVKKVVSYLKDQSGME
                     LDTINLDPQGDKADPNAIFAGGLEDGDDDELYEDARLAVMEAGKASTSYLQRKLRLGY
                     SRAARLMDMLEERGVIGPADGSKPRPVIADGPETEEV"
     gene            23822..24412
                     /locus_tag="VFS75_01900"
     CDS             23822..24412
                     /locus_tag="VFS75_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEU4677455.1"
                     /translation="MLRGDTSIRHTDQIVLDLETASRTVLDASLASLKSTDLTVHESA
                     LSLAYELMNLESKLRVAHAARLIDQGLLDVFVHEIDSVHRALRKYVGTDVRNPLLGDT
                     APERREREGRTPRRSLAPVGDAPLTERAPSPSSVGRRDRVLDVIRTKGQASIKDIVEV
                     VSDVSEKTIQRELNSLIKDNIVHREGERRWSKYSLV"
     gene            25242..27236
                     /locus_tag="VFS75_01905"
     CDS             25242..27236
                     /locus_tag="VFS75_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU4677456.1"
                     /translation="MNITANNAVSKLFVAFVAAAMLFMLAAPSAKAATVEELQAQIAA
                     LMAQIAALSGGSTSGSTAACTFTRSLTVGVSGDDVKCLQNYLTPTYFSNAAGATGYFG
                     PVTQAAVAAWQAANGVTPAAGYFGPVSQAKYSAMASAGGNTGSTGGNTGSTGGNQGGS
                     TSNLSGEADLDDVQIDDADDDTVQEGQDDVQIGQITVKFKNGDARIDRMDIALQGGDD
                     ETDPWDTFDKVMLSVDGDEIASMDASDRDNYLNDDEGSLRFSHLDLVANEDDEVVIDV
                     TADLQSGMDDSNDGEAWSLYVGSVRYFDAEDVATTEDGVGDQVSDFGSVGAAASADFT
                     INEAGNDDEIIVKTSSEDPDATTLEVKDDQKSDWYTVFAFDLDTKDSENDIDLNTIQV
                     GVHTPGTSTYNGLVDDAELVIDGTTIDDVTVTGGGTNDATLSFDVNNDVTINAGDRVT
                     AELKLKFKSLASGDEGATIYAEVSSADRAAIDATGADDLAAGQLSGSATGDTHTLRTS
                     GVAVEDFSADTDVTTSDTATNSYGTFTFTFDVTTIGDDEVFIPLTAASTTASGSTTKG
                     AVYKITDANGTTVTASDSNSASLVRISGGDTSTAGYVRLDSGETATLKLTVTFNPTTA
                     GQFIGVLDSVGFAATADPATDSTTLTPSTDYDTDPAFITS"
     gene            27514..27996
                     /locus_tag="VFS75_01910"
     CDS             27514..27996
                     /locus_tag="VFS75_01910"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEU4677457.1"
                     /translation="MRITDQYFWNVIFLIFFVVLVFLGTVILESEAWKPLSDLTMTDY
                     VLITLASFRIIRAVLYDKIFAFFREQFYDAKEVRGKVILVKPESGPRRTLAELLSCPW
                     CFGIWSAAMVSFFYLLSPVFFYPVLFIALAGVASFLQILSNYIGWKAEEAKQNVEGRM
                     "
     gene            28234..28719
                     /gene="rplJ"
                     /locus_tag="VFS75_01915"
     CDS             28234..28719
                     /gene="rplJ"
                     /locus_tag="VFS75_01915"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU4677458.1"
                     /translation="MAITKAKKQDILAKLENIKKDAEAIVFVNFKGVTVKDTTAMRRT
                     MRDAGVGYFVAKKTLMKRAFGEAYEGTMPELDGEIAVAYSTDAILPAQNVKDFAKQFK
                     DKIAIAGGMFQGVWKSKEEMTEIASIPSLDVLRGMFAQLINSPRQRFAVVLSKVSETK
                     S"
     gene            28746..29108
                     /gene="rplL"
                     /locus_tag="VFS75_01920"
     CDS             28746..29108
                     /gene="rplL"
                     /locus_tag="VFS75_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU4677459.1"
                     /translation="MNKDQIIEAVEKMTVLELNDLVKTIEEKWGVSAAAVAVAAPGAA
                     ADAGEEQSSFNVELTDGGAQKIAVIKVVKEALGLGLKEAKDLVDGAPAIVKEGMKKED
                     AEALKAKLEEAGAKVTLK"
     gene            complement(29253..29585)
                     /locus_tag="VFS75_01925"
     CDS             complement(29253..29585)
                     /locus_tag="VFS75_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677460.1"
                     /translation="MRTRKKRMPEENDDPNAPAWLEIKQLPEPVEKRLERRLQEKRLL
                     SSHVRLDLVDHVFWRTTRGAAKRFLKGLAVSDALSFHLYTKDDLGNYMRVSIERLLEL
                     ESTPRPTR"
     gene            complement(29582..29989)
                     /locus_tag="VFS75_01930"
     CDS             complement(29582..29989)
                     /locus_tag="VFS75_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677461.1"
                     /translation="MTRKTVVRMGAIAATFRPGFKARPAQSVLEYYLLPLSNLVSEAV
                     ALGTIDVKLPQTVTRRVPGYADEQQLYRVDSAVIARVASEPSFSGHYKLFVRMTEGGP
                     LYRYRSDGKKVGAKTRRAKGALKRTRPTVREGA"
     gene            complement(30099..30314)
                     /locus_tag="VFS75_01935"
     CDS             complement(30099..30314)
                     /locus_tag="VFS75_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677462.1"
                     /translation="MATRMSVDMKVTLGIAGTLLVIGGVLMGLALSGTLPRPPIEGIL
                     GGVLPTVLSAAGTACLFFAYRRPQPTT"
     gene            30460..31095
                     /locus_tag="VFS75_01940"
     CDS             30460..31095
                     /locus_tag="VFS75_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677463.1"
                     /translation="MKDSLAILFGNQARVKLLRFFLFNPKQEFTFDDISRRAKLVRRT
                     ARTEINALERAEVIKRKMIYVKHPTKDAKLRVQGYVLNDKFPQLNSLQSFFFETAPIN
                     GTTLLKHLRKAGPLDFLASTGVFMREFDRRLDFLVATKKLNEHRVEIAVRALEAELGI
                     EIRYATFSTEDLIYRVGMYDKLTRDVFDYPHQILVDRVGVRNELLSRPAFA"
     gene            31637..34818
                     /locus_tag="VFS75_01945"
     rRNA            31637..34818
                     /locus_tag="VFS75_01945"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            35009..35081
                     /locus_tag="VFS75_01950"
     tRNA            35009..35081
                     /locus_tag="VFS75_01950"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35042..35044,aa:His,seq:gtg)
     gene            35284..35392
                     /gene="rrf"
                     /locus_tag="VFS75_01955"
     rRNA            35284..35392
                     /gene="rrf"
                     /locus_tag="VFS75_01955"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            35651..36949
                     /locus_tag="VFS75_01960"
     CDS             35651..36949
                     /locus_tag="VFS75_01960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4677464.1"
                     /translation="MRQTITDPGASELVYEIREIVEVAKKFEALGQPITWENIGDPVA
                     KGEEVAPWMKEYVVEAVKENAVYAYSPTKGLQATREYIANERNLEGGIEITPDDILFV
                     NGLGDAISKVYRSLNPKVRVIGPNPAYPTHSSAEAAHADGPHLTYPLDPKNGWKPDLD
                     AMERTIVEHPEIGGILIVNPDNPTGYVYPQDVVRDIVAIAKKHDLFIISDEIYSNLAY
                     GIEHKKLASVIDDVPGIAMRGVSKEFPWPGGRCGWMEFYNRDKDEDFDRFCKSLIDAK
                     MLEVCSTTLPQYVLPRIMKDERYYPMLKERTARYAKRAKLAKDVLSEIPGVIIHEPNG
                     AFYMSLVFEAGVLTDRQTLPVENEAIATELGKLLTGVTNDKRLVYYLLAATGICVVPL
                     TSGFNSTYDGFRFTLLERDDAKFREIIETLRDAIRAYIGS"
     gene            36992..38350
                     /locus_tag="VFS75_01965"
     CDS             36992..38350
                     /locus_tag="VFS75_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEU4677465.1"
                     /translation="MRWWAFWRRVEYGAAFLVLCAGIGTGLYYMYGYEAPTCFDGLQN
                     GDERNVDCGGVCQRICPMDITMPTALWAESFKVVNGQYNAVAYVENRNKDIGSPELSY
                     TFKLFDAQGALITERSGTTVLPPDGVYPIFEGRIMTGDRVPAYTSIEFGEDILWRKGS
                     VGRDQFTLEKRELVNADSLPKLTANLRNSSLDEAEDVEIVATIFDSQKHPLTAARTVL
                     KYFPGRTSEDVVFTWPEPIAKTVKSCEIPTDVALAIDLSGSMDNDGGTPPEPISSVLA
                     AATDFAKQLKEDDQVTLVTYATKAEVVEPLTHESSRVANDISKLAIDPKEQTGSTNTG
                     DALLKIASELGSTRHSADARKVAILLTDGLATAPDDPEVYAIREAEDLKQMDVLLFTI
                     GLGADVNEDFLRAIASNPSMYHKAPSAKDLSSIYAGIKKDICEDGPAVIEIIAKPKTS
                     FQ"
     gene            38388..39506
                     /gene="rodA"
                     /locus_tag="VFS75_01970"
     CDS             38388..39506
                     /gene="rodA"
                     /locus_tag="VFS75_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU4677466.1"
                     /translation="MTLVRTLFKSIDWWLISAVLFLTFLGLVTMYTFDGDNSYFTKQI
                     AWIVIATAGLAIAIVPDYRFLRTGNTTFFIYLGVLVLLVLVLFVGSTVKGAESRFDLG
                     VFSLQPSDPAKLALIAVLAKYFSKRHELIGDFRHILVSGTYALLIFGLVFIQPDFGSA
                     IILFSIWFGMVLVAGIKFRHLLIVFLIGVLSFGVMWQFIFLDYQKQRILTFLHPLADI
                     QGTGYNAYQSTIAVGSGQLLGKGVGYGTQSKLQFLPEYETDFIFAAFAEEWGLLGVLL
                     LFFLFGVVIWRLLTHAVHGATNFERLFAVGVAILIISHFFVHIGMNIGLLPVTGTTVP
                     FLSYGGSHLLTEFLGVGMVIAMGRYTKRHYVGEDLLAS"
     gene            complement(39503..40648)
                     /locus_tag="VFS75_01975"
     CDS             complement(39503..40648)
                     /locus_tag="VFS75_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4677467.1"
                     /translation="MTTNAKKQKTLYIITKSNFGGAQRYVYELATGLPRDRFEPVVAA
                     GGDGILFEKLRAAGVRTVTIASFERDISITKELRSMFELARIIRRERPDVVHLNSSKA
                     GGSGAFVARLLGVRQIIFTAHGWPFYEDRGALWRAVVWFFSWLTALLSHRVIVVSARD
                     RAVVLPFSREKLTLIHTAVPHIDFLDRADARGRLFSAGVVSEHEHDLWLVTNAELTKN
                     KNLFTALDAVSRYNEVRDTKIFYTLISDGELRHELEMYVRDRGLSHAVRFTGYLDDAR
                     TYLRAFDVFLLPSLKEGMPYALLEAGAAGLPSIASDVGGIPEVIENGATGLLIDPRNT
                     DTIVTALETYARSMEKRDACGNALKEKVAREFDIARMLERTFALYGD"
     gene            40887..42245
                     /locus_tag="VFS75_01980"
     CDS             40887..42245
                     /locus_tag="VFS75_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4677468.1"
                     /translation="MHMFVRGSTVLLLIGDLIVFTGSLVLTLFVRYQAWPSHDLVSTH
                     LRAFTVLFVFWVLTFLITGLYDRYISLVRKSIPALVLKVQFFNILLAALIFFIFPFGI
                     EPKTNLVIYLLISTLLIVLWRLYVYPLIATGKPMRALVVGDSDEALAIARMFQGNPFF
                     RNIKPFLLRKKDISDFDEFRASLLRFVDQGSADMVIADMRDEFANRLTRDFYKLAFEN
                     SNIRFFNLPAIYEHLYHRVPPELVEESWLLENVSAGSPHYAYDVLKRGIDLAGAIALL
                     VPALVLFPVIMLAIYLEDRGAFFYEAERVGQFNRPIRILKFRTMTGRDASGDALKSTL
                     TITKVGTFLRKTRLDELPQLLNILMGDLSFIGPRPEIPSLVSVYAENIPYYNMRHLTK
                     PGLSGWAQINNFDVPRGGVDIPRTIDKLSFDLYYLKHRSLILDIEIALKTINTLVLRS
                     GT"
     gene            42295..43200
                     /locus_tag="VFS75_01985"
     CDS             42295..43200
                     /locus_tag="VFS75_01985"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4677469.1"
                     /translation="MSTYLITGGAGFIGTNLAERLVRDGQTVVVVDDLSAGKAERLPK
                     EVDFRKLDICDTATLTAVMKGVDVVVHLAALPRVQFSIEHPFEAQHVNVDGTLSVLEA
                     ARQAGVKRVVYAASSSAYGDQENLPLSEDLPPQPKSPYGLHKYYGEVLMKLWHDIHGL
                     ETVSLRFFNVYGPHFDPEGAYALVIGKFLKQLKEGKPMTVTGDGEQSRDFTHVFDTVD
                     AVVRATVSEQVGKGEVINVGAGRNVSINELTKMIGGPVEYVAPRIEPKHTRADNRRAK
                     ELLGWAPTIPLEEGVSSLKKEFGIA"
     gene            43213..>43795
                     /locus_tag="VFS75_01990"
     CDS             43213..>43795
                     /locus_tag="VFS75_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEU4677470.1"
                     /translation="MNVDGKRLASGVYESLRTRVRSLSRAPRLTIVTASPNLETKKYL
                     ALKERRAAEVGIETNVIVLAESATTENVVTLVANEAARADALIVQLPLPPSIDADRVL
                     AAVPVSRDADGLNPESAVLSPVVGAIREILARHGVLPQGKRVAVVGEGRLVGRPASAW
                     FRSVGAQVRVLTKDTADIEAETRDADIIVSGAGV"
BASE COUNT         9255 a        12600 c        13298 g         8642 t
ORIGIN      
        1 gtcgaagagg gtcctgcccg cgagctcgtc cgccggaccg attgagatgg acacgaggcg
       61 cgcccctagt atattgtgat gcgtatggaa ggagagatac gagagctcat cgacgccgcg
      121 cttgccgcga agggtctcga cgccgtcgcc gttaccgtcg agcacccggc cgatgagtcg
      181 cacggcgact acgcgacgaa cgcggcgctc gtcgccgcga agcgactggg cgcgaatcct
      241 cgcgcgctcg cggaagacct cgtggcccag atggcgggga agtcgccgaa cattgaatcc
      301 atcgaggtcg caggacccgg gttcatcaat tttcacattg cacggagctt cttcgcggaa
      361 gccgtcgcga acgcacgtgc gaagggggac gcgtacggaa cgaacgatac gcgaaagggc
      421 caggagatac tcttcgagta cacgagtccg aacctatgga agccgctcca tgtcgggaat
      481 ctggtcggaa atatagtcgg tgagtccgtc tcgcggctct tcgaagcgga gggtgcgacg
      541 ctccgtcgca taaattaccc gtcggacatc ggcctaaccg tcgcgaaggg cgtctggggc
      601 cttaagaaga cgggcggcga ccccgaagat atcaacgcca ttggcgaggc gtaccgggtt
      661 ggaaacgagg cgtatgaaac cgacgagacc gcgaagacgg agatcgaggc ggtgaaccgc
      721 gcgctctacg cgggcgacga cgaaggacta aaagcgcttc gcgagagggg gcttcggacg
      781 tcgcaccgcc accttatgga gctttgccgc atgctcggga cgtcgttcga cctcgagata
      841 cgcgagagcg aggtgagcga tatcggcacg gagctcgttc ggacgcatac gggcgacgtg
      901 ttcgtggaga gcaacggcgc ggtcgtcttc ccgggtgagc aatatggact ccatacgcgc
      961 gtcttcctca actcgcaagg tctcccgacg tacgaagcga aggacctcgg taacttcgcg
     1021 cggaaggaac gtgcgtaccc ccattggacg cactcgtata tcgtgacggg cggggaacag
     1081 cgcgagtatt tcatggttct cttcgaggcg atgcgaaggg tgttcccgga cactgccgat
     1141 aagatcctcg cgcacatccc cacgggattt ctcacgctca cgaccgggaa gatgtcctcg
     1201 cggaagggaa acgtcctcac cgcggagtcg cttcttgcga gccttatgga ggaggtagag
     1261 aagcgagcga cggagagccg cgcggagaac cttgcctcgc tcgtcgaaga ggtcgctgtc
     1321 ggcgcgctca agtatcagat actccgccag gccgcagggc aggacatcgt cttcgataaa
     1381 gagcgcgcgc tctcgcttga gggagactcc ggcccgtacc taatgtacgc gcacgcacgc
     1441 atgagctcca tcgaggagaa ggccgcggcg gcgggtatgc acgcgggaac ggacgcacca
     1501 gaggtgccgt acgctctcga gcgctttctc taccgctttc ccgaggtcgt tctccgggcc
     1561 ggtcacgagt gcgcgccgca ccacctcgtc gtctacctca ccgaactcgc gagtcgcttt
     1621 aacgcctttt acgccgtgga gaagatcgtc gatgacggcg acccgtatgc cccctataag
     1681 ctctcgctca cgaaagccgc gcgcgtcgtt cttcgaaacg ggctcgcgct tctcggtatc
     1741 gcggctccgg agcgaatgta ggttcacgct tgcttcatgg cctctccggt acgatagggc
     1801 ggaagggaga cagactgtct tccgctttat aaattaacgt gcggctatgg acaacagcaa
     1861 ggatgacaag aagaacgacc gccagcatgg gaaccgctaa gcggtaggcg gagaatcgaa
     1921 catcgaaacg tcgataagag cacgcaagaa cggcgaaccc atcgccgttt ttgtgtttct
     1981 tcgcggatgg cgcgcggttc acgtggattt cactcgtatt cccgtatgct tcacggagtt
     2041 actcgcataa tatcgctagc accatgaaac aagtcgtgct cggactattc tcctcgcgcg
     2101 cccgcgccga ggagctcatt agacatctca cggaatctct cggcgtacga accgatgata
     2161 tctcatacat ctatcggaac acggaaggcg acgtcgaagc cgctccggcg acggacgcgc
     2221 acacgccggc ctccggcgcg gcgaccggcg cgctcgtcgg cgggtccgtc ggcgcgctcg
     2281 cgggcatcgc gaccgctatc ggcgtcattc ccgtcatcgg acccatcttc gcggccggcc
     2341 ccttggtcgc cgcgctcggc atcggtacgg cgagcgccgt cggcacgacg gccgtgggag
     2401 cggcgaccgg cgccgtcgcc ggcggcgtga taggcgccct cgtcaatctc ggggcacccg
     2461 acgtcgaggc gaaggagtac gagaatcgca tcctcgcggg cgacgtcctc gtcgccgtca
     2521 cgaccgatga ggtaccgccg gtccataccg cgttcacgac gtatggcgcg acggacctcc
     2581 ggacctacgg cgcgaagtga aaaacgggcc gcgaccgcgg cccgttttcg tattcgtgct
     2641 atgatagccg ggtacgcctg atatgcgtac gcttatatat gtctgataca catagcgcca
     2701 tggaaaacaa tgaaaagagc gacgccgcgc gtcgcgagga ggaagtactc gcgttctgga
     2761 aggagcgcga catttttcat aagagcctta cgaaggacgc gccgaaaggc gattacgttt
     2821 tctacgacgg acctccgttc gcgacgggta cgccgcacta cgggcacata ctcgcgggca
     2881 ccatcaagga cgcgataccg cgattcttca cgatgcgcgg ttaccgggtg aagcgaaagt
     2941 ggggctggga ctgccacggg cttcccctcg agaacatcat cgagaaaaag ctcggactcg
     3001 cgacgaagcg agacatcgag gagcttggcg tgaagaagtt caacgaggcc gcgcgcgagg
     3061 cggtcctcga atacgaggac gactggaagg aggtcgtgcc gcgcatggga cgctgggtcg
     3121 acatggagca cgactacaag acgatggact cgacgtatac ggagtccgta tggtgggcgt
     3181 tctcggagct cgatcgacaa ggtctcgtct acgagggttt caaatcgatg catctttgcc
     3241 cacgctgcgg gacgacgctt tccaatttcg aggtgaacca gggctacaag gacatcaagg
     3301 atatcgcggt caccgtcaaa ctcccgctcc tcggtacgga tggaaacgtg acggatacgt
     3361 cgctcctcgt ttggacgacg acgccgtgga cgctcccggg gaacatggcc gcggcggtca
     3421 attcggaaat cgagtacgtg aaggtccgcg ttccgataga agacggtgac gaggtcgtca
     3481 ttctcgcgaa ggagcggctt gcgcagttgg ataccgacgc gtacgaggtt ctcgaaacca
     3541 tgaaagggaa ggacctcgtc ggtctccgct acgagccgcc tttcgactat tacaagcacg
     3601 aggagtttca gaataaggag cacgcgtgga aggtgtacca cgcggactac gtcgaagtcg
     3661 gcgaggaggg gacaggtgcc gtccacctcg cgcccgccta cggcgaggag gacctcgagc
     3721 tcgcgaagcg agaaggtatt cccgtcatcc accacgtcgg agaggatggc cgtttcaagg
     3781 acgtcatcac cgatttcaag gggcgcctcg cgaagccgaa ggacgatgac gcgaccggcg
     3841 tgacgcatct cgaagcggac atcgaggtcg tgaaagcgct caaggaacgg ggtcgtctct
     3901 tcaggaagga gaacattacg cacagttacc cgcactgctg gcgctgtgat acgccgctcc
     3961 ttaatttcgc gacgacgtcg tggttcgttc gcgtgtccga gatgcgggat accctcgtcg
     4021 ccgagaacgc gaaaatacgc tgggttcccg agtacgtcgg gcgcgcacgc ttcggcaatt
     4081 ggctcgaagg agcgcgcgat tgggcgatct cgcgtcagcg ttactggggc gcaccgctac
     4141 ccatttggag gaatcctaag acgaaggaac atcgagtcat cggttctttg gccgaacttc
     4201 aggcacgcgt tcgaaagagc ggtaatacgt acgtcatcgc gcgccatggc gaatccgtgt
     4261 tcaatacgaa gtcgctccta agctcgctcg cagagacgga gaacccgctt agcgccacgg
     4321 gccgagaaca ggtgaagcat gccgcaaagg cgcttcgaga gcgcggcatc gacgtcatct
     4381 acacgtctcc gctcggtcgt acgagggaga cggcggacat gtttgccgag gcgctcggta
     4441 tgccgaggga gcgcgtcatc gaagacgagc gactcggtga ggttcgcttc ggcgagttcg
     4501 aggggaaact gatcgacgaa taccacgcgt acttcaagga cgggcatgaa cgcctcacga
     4561 agaggccgga gggcggcgag tcgtggaccg agttgaagcg tcgcatgggg tcgttcctct
     4621 acgatatcga cgcgaagcat acgggggagc gcgttctcat cgtctcgcat aacggtccta
     4681 ttcagatgct tcgcgccgcg gcggcgggag acgacagagc gacgaccgcg aagcgcatcg
     4741 acgacaacgc gtacgacgtc gcgaacgccg agctctctga tctcgcgttc acgccgatgc
     4801 cccacaacga cgacttcgaa ctcgactacc accgaccgta catcgacgag atcgacctct
     4861 tcgaccccga tggcaggaag ctcgagcggg ttcgtgacgt cttcgactgc tggttcgagt
     4921 ccggctcgat gccgtacggc cagaatcact acccgttcga gaacctcgac gcgttcgacc
     4981 cggggaaggg cgcgggatat ccggccgagt tcatcgccga ggggctcgac cagacgcgcg
     5041 gctggttcta ttcgctcctc gtcctcggga cggcgctctt caagcgcgcg ccgtatcgcc
     5101 atgtcatcgt gaacgggctc gtcctcgccg aggacgggaa gaagatgtcg aagcggcttc
     5161 agaactaccc cgaccccatg gcgctcgccg accgcgtcgg cgtcgatgcg atgcggttct
     5221 acctactctc gtcgccggtc gtgcgcggcg aggacctcaa tttctccgag aaggaggttc
     5281 gcgagctcga gcggaagaac atcggccgac tccacaacgt cctcgcgatg tacgagatgt
     5341 acgccgacgg caccgcggcg tcggactcct cggacaatgt cctcgaccga tggatggtcg
     5401 cgcgccttcg cgaggcgacg aaggagatga ccgacgggta cctcgcgtac gagctcgacc
     5461 gcgcgacgcg ccccatcgag ggactcattg acgacctttc cgtctggtat cttcgccgtt
     5521 cgcgcgagcg tctcaagggc atcgacgcga aggaccgcgc gcgcactctc tcgacgcttc
     5581 gctacgcgct ccgcaccgtc gcgctcctca tggcaccggc gatgccgttc tacgcggagt
     5641 acctcttcgg gcgcgtgcga gaggcggggg atagggagag cgttcacctc atggattggc
     5701 ccgctagcgt ggagcccgac ggccacatca tcgccgacat ggccgaggcg cggcgcgtca
     5761 cgtctctcgg tcttgaggca cgctcgaagg cgaacatcaa ggtgcgccaa ccgctcgcgc
     5821 tgcttcgggc tcgggcgaac gctcgcgtcg aatatctcgc ggacatcgtg aaggcggaac
     5881 tcaacgtaaa gcgacttgag atcacgccag atctcccggt cgacgtcgaa ctcgatacgg
     5941 agataacctc cgaacttaga cgcgaaggcg atgcgcgtga gcttatccgc gcggtccaag
     6001 acgcgcgcaa ggaggaaggg ctcgacccgc gcgatcgcgt gaccctctat gtcgacggcc
     6061 gcacgcgcga cctcctcatg ggcttcgaga aggagctctc gcacgtcgtc ggcgccgagg
     6121 agatcgcggt gggcgagacg acgaaggagg tgaagaccga gggagaaccg ctccggttct
     6181 ccatcgcgaa gcgataatgg cctataagac gtacatcacg aacgcgctcg tttgcgccgg
     6241 gttcgacagc catagcgcgg accgctcgtt ccttcttttt acccgtgaag cggggatgct
     6301 ctttgcgagc gcgaagggcg ttcgcaaaga gatctcgaaa caccggtacg cgctccaaga
     6361 gtgttccgag gcgcgcgtca cgctcgttcg cggaaaggcc ggctggaggg tgacgggcgc
     6421 ggagccggtg cgtaatttct atgcgatggc gaggacgcgc gaagcgcgcg cgcttctcaa
     6481 gaacgccatt ctcctcatac ggcgagtcat gcacggagag acgccgcacg ctggcgtctt
     6541 cgacgatgtc atcgcgtcct tcgagcggat gcatgaccat cgctatgaga ccctggggag
     6601 cgtgctctcg ctccgcgttc tccacgcgct cggatacatc gccccctcgc cggaatatga
     6661 cctattcctc tcgccgacgt tcccgttctc cgcggtcggt gacctcaagg acgagaccgc
     6721 cgaagcgctc gagcgcgcgg tcgaacgcgc gctcgtcgag agccacctct gacccggagg
     6781 ccctgtgtta tactcgtaga tatgtcgaac gaggaagcgc gacgagaaca acccgagccg
     6841 tcggacccgc gtcgagtccc cgaggatcgc atcaaagagc ttcgcgaacg tctctacgcg
     6901 cgtggcgatg cgcccacgga cgtacggcac aagctccccg agcgcccgca tgagccgctc
     6961 cgctcggata tccagccggc ccggcccgcg cccgaacctg aaacggagcg agcggaaaag
     7021 cctcgggaag agcagacggt accgtatact agacccatgg ccgcaaacag catgcgaagt
     7081 tcgtttcgta agaaattcct catattcggc ttcctcttct tcgtcatcgc gatcgggctt
     7141 tccgccgcgt tcatgttcct cggcacccat tcggtctcgg gcgagaacat cagcgtgaac
     7201 gtcaccggac cgcttaccgt gaacggcggc ggggagtacc ccttccaggt atcgatcgcg
     7261 aaccagaaca ccgtccccat ccagtccgcg accctcattg tcgaatatcc cgcggggacg
     7321 cacagcgcga ccgaccccaa tgcggaggtc tcgatcattc gtcagccgct cgatacgatc
     7381 ggctccggtg agatcgtgaa cataccgctt cgcgcgcgta tcttcggtga ggagaacgac
     7441 gaaaaagaga tcaaggtctc cgtcgagtac cgtgtcgaag gctcgaacgc gacgttcgag
     7501 aaggaagcgg cgccgctctc gttcaaggtc ggcacgtcac ccgtcgttgt caccatagat
     7561 ggcctcaaat ccatctcttc gggacaggaa ctcgacttca ccatcaccgt ccaatcgaat
     7621 tcgcccgcgc cgctctcgaa tcttctcctc aaggcgacgt acccagacgg tttcgatttc
     7681 acgtcgtcga agcccgagac gctcgcgggc gaggatacgt ggggcatcga tacgctgaag
     7741 ccgggagaga agaaggtcat caaggtgacg gggctcatga cgggctacga ggacgaaacg
     7801 cgctcgatca ccgtcgaggc cggcgttccc gccggagcgg acatcagcca tatcgcatcg
     7861 atgctcgcct ccgcgaccgc ggacgtgaag atagagaagt cgttcctcgg cgtcgacctc
     7921 tcggtgaacg gtatggagac ggaaccggtc atcatgaacg cgaccgaggt cgcgaacgcg
     7981 cgcgttaccc tcacgaactc ccttgatacg gcgatctacg acggcaaggt gaccatcgaa
     8041 ctcacgggga acgcgctcga cgagttcaac gtgaagtcgg cgggcggatt cttcgattcc
     8101 tcgaagaaca ccatcacatg ggacagcgcc gagttcgaga acctcaagga gatattgcca
     8161 ggcgaggacg tcatcctcgg attcacgctc gagccgaaac agaacgtcgg taccgcgccg
     8221 gagatccacc tcaaggtcac ggcggagggc tcgcgcgtct tcgaggaccg cgcgccggag
     8281 aagctctcgg gcatcgcaga gcgtaccgtg aagatcgaga gcatctcgtc ggttgatgcc
     8341 gccgcgtacc atgcgaccgg accgttcacg aacactggtc ctataccgcc cgtcgccgaa
     8401 gaggtcacgc agtacacgct caccttccgc gtgaagaccg gcgcgaacga cgtgaccggc
     8461 gcggaggtga ccgcggcgct cccgcagtac gtgagctggc tcgacctcgt ctccgaagga
     8521 gccgacgtct cgtacaatcc gacgacgcga acggtgaaat ggacgctcgg caaggtcgac
     8581 gcgaacacga cgaaggagac gtcgatgcag gtgtccttca agccgtctct ctcgcaggtc
     8641 gggacgatgc cgaccattct cgaagcccag cgcttcaagg cgacggaccg gttcaccggt
     8701 accgtcgtcc gcgcggagca tccggccgtc acgacgagcc tcacgaatga gcccgactct
     8761 gccgccgagg atggcagggt tcgcgcgagc gagtagcgca tttgctcgcc gctccatatc
     8821 tctatatagt gtaggaatta cgcggtaatc ccgtttccat ggaacacagc gccgaaaaga
     8881 tggagaagat cgtgtcgctc tgtaagcggc gtgggttcgt gttccaaggc tccgagatat
     8941 acggcggctt cgcgggggcg tatgactacg ggcccctcgg cgtcgctctc aacaacaacc
     9001 tgaagcgcga gtggtggaag atgttcgtcg aatcgcggga ggatatgtac gggctcgacg
     9061 ccgccattct catgaacgag aaggtgtggg aggcgtccgg ccacaccggc gcgggcttca
     9121 gcgatccctt ggtcgagtgc gagaaatgca agcgtaggtt ccgcgcagat caccttgaga
     9181 acaaggagac gtgtccggag tgcggcggga agctcggcga ggagcgccag ttcaatctca
     9241 tgttcgagac gaaggtcggt gcacagggcg acgcgaagtc gtatcttagg cccgagaccg
     9301 cgcagggtat cttcgtcaat ttcaagaacg tcctcgactc gatgagcccg aagctcccct
     9361 ttggtatcgc gcagataggg aaggcgtttc gaaacgagat cgcgccccgc gaattcctct
     9421 tccgcgtccg cgagttcgag caggcggaga tcgagtactt cctcaaaccg gaggattggg
     9481 agcgcctctt cgaggcgtgg cgcgaggacg tgtggaagtg gatcgaccac atcggcgtcg
     9541 cgcgcgagca cgttcgcgag ctcgaggtcg acgcgaacga cctcgcacac tactcgaagc
     9601 gcaccgtcga cttcgagtac gactacccgg ggaagggttt cgccgagctc ctcggtctcg
     9661 cgtaccgcac cgaccatgac ctccgctcgc atatggaggc gtccggcgag aagctcgagt
     9721 atatcgagcc ggatggcacg cggttcatcc cccatgtcat cgagccctcc ttcgggctcg
     9781 gtcgcgcgtt catcgcggtt ctcgcggaag cgtacacgga ggacgagatg aacggcgagg
     9841 tgcgtacgta tctcaagctt ccgacgcacc tcgcgccgta ccgcgccgcg gtatcaccgc
     9901 tcctgaagaa caaacccgag ctcgtcacga aggcacgcga ggtcttttcc atgctcaagc
     9961 aggaattcgg gaacgtcgcg tgggacgaca acggcaacat cggaaagcgc tataggcggc
    10021 aagacgagat cggtacgccc gcatgcatcg tcatcgattt cgagaccatc gagggagaga
    10081 cgaaggatac ggtgaccgtt cgcgaccgtg acacgggcgc acaggtgcgc gtcccgatcg
    10141 cagacctcgt gtcgcacctc acgagcgcgt aaatttcatt atggcgaaca agtatctcac
    10201 cacgacgctg ccgtacgtga acgccgaccc gcacgtcggc ttcgcgtacg agctcgtcgc
    10261 tgccgacgcg ctcgtccgct actggaaggc gaccggagac gacgtcttct tcaatacggg
    10321 aacggacgag cacggtcaga agatagccgg gaaggccgac gagaaggggg agagccgcca
    10381 ggcgtacgtc gaccactacg cgaacgagtt tcgaaaattg ataccggccc tcggcgtttc
    10441 cgaggacatc catttcatac gtactaccga cgaacgccac aagaaggcgg ccgaggagct
    10501 ctggcgccgg tgcgaggccg cgggcgacat ctataagaag aagtacaagg ggctctactg
    10561 cgtcggcgac gagatgttcg taaaggagag cgagctcgtt gacggcaggt gcgcgaatca
    10621 cccgaacctc gaactcgagg agatcgagga ggagaactac ttcttcaagc tctcgaacta
    10681 ccagtcgtac ctcgaggaat acctctctcg ggacggtgtc atcgtccccg agtggcgacg
    10741 gcaggaggcg cggagcttcg tgaagaaggg tctcgaggac ttcagcatct ctcgcgagaa
    10801 ggcgcgtctc gattggggca ttccggtccc gggcgatgag aaccaggtca tgtacgtatg
    10861 gttcgacgcc ctcacgaact acgtctcgac cttgggctgg cccgaggata ctgaaggaaa
    10921 ctacgagaaa ttctggaaga gcggcgaacc gatgcagata gcggggaagg accaggtccg
    10981 cttccagtcc gtcatgtggc aggcgatgct caaatcggcc ggtctcgcgc cgaccgcgaa
    11041 ggtcctctac cacgggttca tcacctcggg aggtcagaag atgtcgaagt cattggggaa
    11101 cgtcgtgagt cccttcgagc tcgtctcgcg ctacggcaca gaggcgacac gctacctcct
    11161 ccttcgccac gtccatccct tcgaggatac tgacgtcacg tgggagcgcc tcgacgagtg
    11221 gtacaacgcg ggactcgtga acggcatcgg gaacctcacc gcgcgcatcc tcaagatgtc
    11281 cgagacgcac ctcggcgcgt tcgaagccca cgacttcata tctgagatga agggcatgga
    11341 ggagtatgac tgtaagagcg cgctcgacta cctctggtcg cgcatccagg cgctcgatga
    11401 gaagatagcc gagaccgagc ctttcaagct cgtgaagacg gaacccgaac gtgcgaaggc
    11461 gatcctcgag gaactgcgcg ccgacctcta caggctctcc atcgaactcc ggccgttcat
    11521 gcccgcgacg gccgtgacga tagaggaggc ggtcaaagcg aacaagaagc cggagaatct
    11581 cttcgcgcgc atcgactgat atgaggaagg tgtacaacaa gctcgttcgg gacaagatga
    11641 tcgacgtcta caagcacgac gtcgagaaca agataagctc gagcgattac agcgttcgct
    11701 acctcaatcc ggcggagacc ttggaggaat taaagaacaa actcctcgag gaggtggcgg
    11761 aggtcttcga ggcgtacggg aaggacaggg aacacctcaa ggaggagatc gccgacgtca
    11821 tcgaggtcat cgacgcgata ctctttcaca acggcatgac cctcgacgaa gttctcacaa
    11881 tacgcgacgc gaagaaggcg aagaaaggcg gtttcgagac cggcctgtat ctcgagagca
    11941 tcgattactt cgatagcgcc gaataagaaa catacgatta atcgtatgtt tcttattctc
    12001 ttgtgaattt gagcaaggtt ttcttccagc gtcctcggcc cctcggagga aagcccatct
    12061 gcaggatcct ttccagcgtt tccactatcc gagtgtattc gcctttgtct tccgttacga
    12121 accgtcgtat tgcgccgtat ttcccaattt cataattgag ccttatacgg tcggtggtag
    12181 aggggctgag attgagaagt acggacactc gatacgaaga gacgccttcg gtgtagagga
    12241 tgatcgccgc gagtcgtttc gcgaacatga gccgctcttc ctctccgaat agttcgctca
    12301 gaaacgcgga cgctcgtttc gaatcgagtt gcgcgacaac cttgtataac tgattcaaga
    12361 gttggtcttc cttcttatgt ggtagtttac gtttggagac atgtgtcata tcacaatcat
    12421 acgattaatc gtatgattgt gatagggaga gcgtgtgcta tgctggttcg caatgacgga
    12481 gaacggaaaa ggtatcgtgg acatcggtag actcatcctc gcgttcgcga aggtaaaccg
    12541 cgcaacgacg atggaggacg gcgtgacgcc ggaatcggac accgaccaca cggtcatgct
    12601 ctccgtcgtc gcctctgcct tcgccaagaa gctctacccg gactcgctcg acgtggggcg
    12661 agtcgccgag ttcgccatcg tccacgacct cgtcgaagcg tacgcctgtg acaccgatag
    12721 ttttggcatc agcgcggagg caaagcgagc gaaggacgag cgagagcgcg aagcgtacct
    12781 tcgcatcgag cgcgagtttc gggacacgtt cccgtggatt cccgagacca tcgcggcgta
    12841 cgagcgcctc gatagccgcg aagcgcgttt cgtgaagacc gcggacaagc tcctgtcgaa
    12901 ggtgacgcat atcctgaacg gcggcgcata cttcaagtcg cgcggcatgg atgaagagac
    12961 gatgtggaat aattaccagc agatggtgcg cgccgccgag gagaagtacg gcgcggaatt
    13021 cccggaggtc ctcgccctca tggacgagct cattagggaa gcgaagcgaa tcgcctatgg
    13081 agcataagac cttcaaggac atctacgcga tagcggaacg gaacgtgaag gagaatccgt
    13141 acgtacgcga aaaggaggcc gatgagatca tcggtatcta tctcgattgg cttcgcggcg
    13201 aggtagaaga agtgagggat gaaacgaaac caaggaatag gatacatctc gccgatgagc
    13261 tcgcagatat actctgggtg tacgcggggc tcttagcagt tctcaaggat agagggtata
    13321 tcgattcggt cgaagaggtc ttcatgcagt cgcacgagaa gtataccgag cgtatgccgg
    13381 catttttacg aagctccgct gagctgtggg aatccatcaa ggcgaggcag aaggaagacc
    13441 tcgccctgcg tcatcaaaag gaatatggac agtgaatttc gatacatcga cgtgcacacg
    13501 caccttaacc tcgatgcgtt cgacgctgat cgcacggcgg tcgaaacgag gactctcgaa
    13561 gaccgcgtcg cacacataaa cgtagggacc gcgcgggaaa caagcgaggc ggcgattcgt
    13621 ctcgcagagt cgtttaatgc cggcgtctac gcgacggtcg gcctccatcc cgttcgcgcg
    13681 agcggcgcat cggagcacga gggcacggag gcgttcgacc acgaggcgtt tcaggcgctc
    13741 ggagcgcatg agaaggtcgt cgcgatcggc gagtgcggcc tcgactactt tcgcgtcgag
    13801 aaagatacga aggcgaagca ggagggggcc tttagcgcgc agatagcgct cgcgaacgag
    13861 ctcgaaaagc cgctcatgct ccacatccgc gacgagaagg gaaagatgga cgcgtacgag
    13921 gatgcgctcg ccatactgaa gcgcgaggcg aaggttctcg ggaacgtgca cttcttcgcg
    13981 gggacgtacg acatcgcgaa gcgcttctgg gagatggggt acacgacgtc cttcaccggc
    14041 gtcgtcacct tcgcagatga gtacgatgac atcattaaaa acgcgccgct cgacatgctc
    14101 cacgcggaga ccgatgcgcc gtacgtcgct cccgtgccct ttcgtgggac gcggaacgag
    14161 cccgcgcacg tccgcgaagt gtacgcgcgc atcgcggcgc ttcgaagcga ggaccctgaa
    14221 gtagtgcgcc gggcccttct cgagaacgcc cgtcggctct tcggcgtcct tatttgacag
    14281 tggcgtcctg aacgcttacc ttgtttcaag acatacttcg gagagagacc atggaaccgg
    14341 gagaagagac ctgcggcgtc cttacgtcgg acatccacat caccggcggt tcggcagaac
    14401 cgctcgaatg ccttcgaccg cggcaccagt tcggccaaca cctcatcagg cgcgacgacg
    14461 gaggctacgt cgcctacgaa acggattgga cgtgcggctg cgagatgtgc cagtccgatg
    14521 agccggacga ttggtgcgag atctatcgcg acgtcagcga cgaggaggcg aaataccttc
    14581 tcggctctgc ggacgcggag ctgaagggtt tccaagactg aacgaacgtt cagtcttgat
    14641 tttttgacat cctgtggtta aatcttccga gaccattacg atggaggaac gagcatgcgg
    14701 cgcgtctatg acctgtcgtt cgtatcgaaa gacgtctcga atagcggcgc gccggagatc
    14761 atcatctcga tccaaccgga gcacgtcacg cagttcaagg tgggcatcgg cgagtacgcc
    14821 acgcggtatt ccgagaacgc ggagcggtat ctcggcgaaa tgccgaattt cgtctcgccg
    14881 acgaattgtc gtttcggcta ccgggggtgc ggcgaagtgc gagagggaag gacgctcgat
    14941 atcgcgttcc cgctctacga tttcgatggg aggcatgtgg cgtacacgct catggtgacc
    15001 ttcgaagagt tgaggacgtt ctttccggcg acgcgcacgg cggaacttcc gagcgagaag
    15061 agccagtacg tcgacgtctc actgccttgt aggcaggcgg tccacggaca caatccgagc
    15121 ggctggattc tccctcactt cggtatgtgg cttcgagcga agggcggaga accgagagaa
    15181 cagggtaccg ttcggagaaa ggtcacgcag gcgatcttcg aagtatggaa cgcgctcgta
    15241 catcgcaggc agaagcaggg cacacagagg gcagtcctcc tcatcgggga caacgggcat
    15301 ttcgtgctcc actgtcccgg cgatgcgtgc gacctcggcg tctatcctga tctcgtccga
    15361 gaaggaatgg gaacgcggcc cgttcggttc gagtcgcata acgtcgagtc gtacgtgcag
    15421 ggtctcgcgc tcctctcggg gctcgctgcg cttgcgacga tcgctcgcga ggagaacgag
    15481 catgtccacc agcattagca cctttcgaag aggcgcctca cccgaggcgc ctctttttta
    15541 atcgaagatg tggaggaggt acgtatcgat ggtccgctgt tctatatcgt agaaatactt
    15601 gtcgtcgaca tggaggtcgt gcgcgaggtc ggtcccgacg aagcgccagt gccacggctc
    15661 atagacgtag tatccgttgt tctcggggta cgagatgacg aatccgtacc ggtacgcgtg
    15721 cttttgaagc cacgtgtacg cggcggtatt ctcgaagccg gtgagtccgc cgagcgcggc
    15781 ggtcgtgaag tcgacggtgg tgccgagttg atgttccgag tatccctggt ccgcggagaa
    15841 cgtgttcgca cccgagccgt attgtaccgt gtacgcgctc ttcagcccct tctgctcctc
    15901 gtacgagcgg tacgcggaga ggacgaggag atcgacgccg tcctccttcg cgtcagagag
    15961 catgtcttcg aggaagggga gcacttcact ctcgatgtat tcgggttcgt tctttcttag
    16021 acggtcctct tccggtatct cctcgagctt cgcaggtttg tagttctcgt tgaggaagta
    16081 gaccttggag tacttcttga ggagttcggg gtcgagtttc gacagtttgt cgagcttcgt
    16141 cacgtccttc gtgatgctcc gtatctggtc ctcgaagtcc tcgttccggt tcttctcctc
    16201 ggagagttgc ttgagcaggt cggcacccgt cgcttcggag gtcgcgtacg cggccttgag
    16261 ggcctcgagc gtcgcctcat cgtcggcgaa ggtagcctcg cgctccgaga gggtcgtccg
    16321 tacggcgtta aggtcgttcc cgagattcgc gttctcgctt tcgagcgtcc ggatgacgta
    16381 gagctcgtag cctgaaacgg cgaagagcac gacgatgagc gcggcgcccg atatgagcca
    16441 tattcttagc ttccgttccg agaggtccac catgtgcctc ccattctagc atgccgatcc
    16501 ggcattccct catccttgaa tcgaccgcgt aactatggta cggtaacgcc acattattag
    16561 ataacgtcta cgggtgcggc cataatcaaa cggtcgtaca ctcactatga cagaatcaac
    16621 aatgccagca gccgaggcgg ccgcgcagtc gtacgatgaa cgaaacgact acgagttcgc
    16681 cttccacgta cttccaacgg ctactgaagg ggaagtgacg agcgccttcg aggccatcaa
    16741 ggccgagatc gcgaaggagg gggagattac cggagaggag gcacccgagc gaatcgagct
    16801 cgcgtacccc atcgtgaagt ccatcgaggg caagaaccga aagttcgcga gcgcgtactt
    16861 cggttgggta cgtttcaagc tcgaggcgga gaagctcgag gccgtgaagg aggaactcgc
    16921 gtccgtgaac gtcatccttc gccatctcct catcaagctc actcgcgagg aggtagcgga
    16981 gccgttccgc tttcacgaga accgaaaatc ggtgaagatg gtggaagtgg tgaacgagga
    17041 gccgcaggcc ctccccgagg cgccgcgaga gaccgaggca cgcgccgagg tatccgcggc
    17101 ggcgctcgac gaatcgctcg agaagataac gaaggacgag ggtgagaaga aggaggagtc
    17161 cgcatcgtaa ttattggttt tagcgaatcg atatgtacct taacaaggca atggtctacg
    17221 ggaatctcac gagagacccg gaactcaagt cgctgccgag cgggatgcag gtgtgttcct
    17281 tcggcatcgc gacgaaccgc gtgtacaagg accgagacgg gaagcgagcg gaacagaccg
    17341 attaccacaa catcgtggtc ttcggtcggc aggccgagac gtgcgcacag tacctcaaga
    17401 aaggtgcggg cgcgttcgtc gagggccggc tccagacccg aagctgggag caagacggcg
    17461 cgaagcagta ccggaccgag ataatcgccg accgcgtcca gttcggaccg aagtcgaccg
    17521 gcggcggcgc accgatgggt gcgagcccga aggccgaacc ggcgggaaac gctcccgagt
    17581 acccggaaga ggagatcaat cccgaggaca taccgtttta gagtcatatg gaaaaagaaa
    17641 agaagcagaa gacgacgaac gtcgagcact acgactacaa ggacgtgcag cgcctccgcg
    17701 cgcacatgaa cccgcatgcg cgtatgatga gccggaagcg aaccggccac aacgcgcacg
    17761 aacagcgagt cttcgccact gcggtgaagc gcgcgcgatt catggcgctc ctcccgtacg
    17821 tctcggaata acctagaatc aacgagcgca gcgactatga ttctagagtc cgtcggtgca
    17881 tgcgtccaca aagacgcctt gaaccgacgc cataatcgaa cgctcgatag agtttcacac
    17941 tcaaaaaacc gccgacccta cgggccggcg gttttcgttt tacgctttct ccacccgttc
    18001 ggtgcctaga attccttaag agcgaagcga atcagaattc ttggccgtca ttttgcgcgc
    18061 cttcgaagcg cctcaaaatg acgaacaaag gttcttatac ctcacgcgca agatcacgct
    18121 ttctccaccc gttcggtgta ttcagctgat tccgtattga tgcggatgac gtcgccctcg
    18181 ttgatgaaga ggggaaccat gatcttcgcg ccggtctcga ggatggcctc cttaagggca
    18241 cccgacgacg tattgccacg gaccgcggga gcggcctcct tcaccttgag ttcgaccttg
    18301 atcggcatct tcacgccgat tatcttctcg tcgaagacga ccgcgtcgat gacggtcttc
    18361 ggcttcagaa actttatctc gtcgccgacg atgtcgccgg agagcgcgaa tcgttctgcg
    18421 ggcttacccg gcgtattgaa ccagtactcg ccgcggttct cgtagatgaa ctcgatcggc
    18481 cgcgactcga tgtccgcctc ctccgcggtc tcgttctgat ggaacgtctg ctcgatgacc
    18541 cgtcccgaga tgaggctctt cagcttcgtg atattgacag gctttcgctg ttgttttcga
    18601 aagacgtgcg atgcgagcac gatatggggc tcatcgttcc aaagtatgac ttttttgagg
    18661 gtgatctcgt tgtaggaaag gacagacatt tggtagtggt gtactatgtg ggtaaatagg
    18721 caggcatagt aacaggttat gttcgggctt tcaaccggag acagcgcgcg agacctcacc
    18781 gatgaggcca tcctttcgcg cgtacagcag gagccttggc tcttcaccgc gctcctcgaa
    18841 cgctaccaag ctgcttttct ccggaaggcg aagaccatcc tccacaagaa cgaggacgcc
    18901 gaggaggtcg tccaggatgc gttcacgaag atttacatga acgcgcaccg tttcgaggtg
    18961 cgaccacgcg cgaagttctc gtcgtgggca tacgccatac tcataaacac ggcgctcacg
    19021 cgataccaga agtgcgtgcg ggaggggaag cggaccatgc tcatcgaccc cgaatacatg
    19081 gaacagctcg gcgagatgaa ggagcacaac gccttccggc aagataagga cgcgatcgac
    19141 cggctcctcg agaaattgcc cggtcacttc gcgcgcgtcc ttcggctcca ctacctcgag
    19201 cattggtcgc acaaggacat cgccgcggaa acgggggagt cggtcggcgc cgtaaaggcc
    19261 cgcatccatc gcgcgaaggc ggagtttaga aagcaggcgg gcgcggaggg cgcgtcactt
    19321 ttggggtaag agcatcgtta tataaacgta acgctaattt tatcatcgta tgaaaaagct
    19381 tcagcggaag atcatgagag gcatctacta cgcgtacgcg cttcgtctcg tctcgctccc
    19441 gggcgtgctc caggggttct tcatgctcgc catcatgata gcgctcacac gcttcgtctc
    19501 ccttggccac gtcatcgcga acttcgagac gggcggtttc gcgcacgtcg gcacgttcct
    19561 ctacaatgcg gtgcgtacga cggagatatg gacgcttctc ctcatcggac tcttcgtctt
    19621 ctcgctcctc tcgcttcgta tcagcctcgt accgcgaagg gaagaggcgt actcgttcgc
    19681 gcgcatttag cgcttcgata tgaaaaccgc cggcccgtag ggccggcggt tttcatatcc
    19741 gcctacgcgg cggtaccctc gtccttcagt gcctttcgaa cgtgcttgag gagctcgttc
    19801 tggagtttct tgtcctcgcg aagtgtcgtt cgcgccgcat cgtagccacg gccgagcttc
    19861 acctcggcgc tctggtcgcc tcccgggggg aggtacgagt aagccgcgcc ggacttcttc
    19921 acgaggccgt acttctcgcc gagggcgagg agctcgccct cgcggctgat gccctcgttg
    19981 tagatgaggt cgaactccgt cacgcggaag gggggcgcga ccttgttctt cacgaccttg
    20041 acgcggtgtc tgccgccgac gacctcatcg cccttcttta tctgggcgat gcgtcgaatg
    20101 tcgaggcgca ccgatgtgta gaacttgagc gccttgccac ccggcgtcgt ctcggggttg
    20161 ccgaacatga cgcctatctg catgcggatc tggttgatga aaatgacgat ggtcttcgac
    20221 ttcgcggtga tcgcggtgag cttccgaagc gcctggctca tgaggcgcgc ctgcttaccg
    20281 acgtgcgtcg cgcccatctc gccctcgatc tcgtccttcg gggtgagtgc ggcgaccgag
    20341 tcgatgacga cgatgtctat ctttcccgag cggacgagcg actccactat ctcgagcgct
    20401 tgctcgccgc tctccggctg tgagatgagg agctcgtcga gcttcacgcc gaggcgcgcg
    20461 gcgtactccg ggtccatcgc gtgctccgcg tcgatgaacg cgcaaatgcc gccctgcttc
    20521 tgcgcctccg ctatcgcatg gagggagagc gtcgtcttgc ccgaggactc gggaccgaat
    20581 atctcgatga tgcggccgcg cgggtagccg ccgatgccga gcgcgtcgtc gagcccgatc
    20641 gaacccgagg gaatggcatc gatgtcgacc ttcctcgtgc tcccgagggt catgatggcg
    20701 tcgtcgccga acttcgtctt gatgcttcgg agcgtctccg cgatgtcgcc catacccttc
    20761 gtctccttcg ccggcgcttc cgcgtcgcgt tcgatggtcg tctctccctt cgtgcttttc
    20821 ttgggcatgc tagatcttct tatccgcgaa gtacgcttcg cactttataa tattcgccat
    20881 tataccctag gactctgggt catacacgta ccgttccgtg agggtggtcg tccggccgaa
    20941 gcggtctttc gccgcgaggg tgacgatggt caatccgttc tcgaggatca ggtcctgcgc
    21001 gaactcaccg gcctcgttcg tgtgtatctc gcggccgttt aacgtcagtt tgacgatgtt
    21061 ctccgcctcg ccgcggagag tgaccgtgcg gtcccgctcc acgagaggag gctcttcggt
    21121 gagcgttatc gagggtccgt gaatgaggtt tcgtgcctgg aactcgacgt acccgacgat
    21181 accgatgacg aggagcgaga cgccgaggaa gcgaaggagg atgccgaggg tgatgttcga
    21241 gaggatgacg cgcatacggt tagacctctt cggtctctgg tccgtcggcg atgacggggc
    21301 ggggcttcga gccgtccgcg gggccgatga cgccgcgctc ctcgagcatg tccatgaggc
    21361 gtgcggcgcg cgagtagccg agacggagct ttcgctggag gtacgaggtc gacgcctttc
    21421 cggcctccat gaccgcgagg cgcgcgtctt cgtagagctc gtcgtcgtcc ccgtcctcga
    21481 ggccacccgc gaatatcgcg ttcgggtcgg ccttatcgcc ctgtgggtcg agattgatgg
    21541 tgtcgagctc cataccgctc tggtccttga ggtacgagac gaccttcttc acctcctcgg
    21601 tcgtcacgaa cgcggactgg aggcgcgtcg gcttcgggct ctctgccgag aggaagagca
    21661 tgtcgccgcg gccgaggagc ttctccgcgc cggactggtc gaggatggtc cgtgaatcta
    21721 tctgcgaggc tacctggagc gcgatacggg tcggcacgtt cgccttgatg aggccggtga
    21781 tgacgttgac cgatggtcgt tgcgtcgaga gtatgaggtg gatgccgacc gcgcggctca
    21841 tctgcgcgag gcggacgatg cacgcctcaa gctccttcgg gtacgcggtc atgaggtcgg
    21901 cgagctcgtc gatgatgacg acgatgtagg gaagcggctc ggggagctcg ccacgctcct
    21961 cctcgggaga gcccttcttc tcgaacttct ccttcgcggg gtggtacacg tggtcgtggt
    22021 acgaggcgat gttctggacc ttcgcctcgg aaaggaggtc gaggcgccgc tccatctcct
    22081 tgatcgccca gcggagggcg acgatgacct tcttcgcgtc ggtgatgacc ggcgtgagga
    22141 ggtgcgggat gccgttatag agcgtgagct cgatgcgctt cgggtcgacc atgatgaagc
    22201 ggagctggtc gggcgagtta cggaagagaa gcgagatgac gacgttatgg atggcgaccg
    22261 actttcccga gccggtcgta cccgcgatga ggccgtgcgg catgcgcgcg atgtccgcga
    22321 accacgcctc gccggtgatg ttcttcccca aggcgacgag gagcggcttc gggctgtcgg
    22381 tgtattccgg ggaagagaag agggacgcga ggccgaccat cgccttttgg cggttcggga
    22441 cctcgatgcc gacgagcgac ttcccgggta tcggcgcctc gatgcggatt gggctcgcgg
    22501 cgagcgcgta ctcgaggttc gtctggaggc cgactatctt ctcgagcttc acgccctcgg
    22561 ctggcttgat ggcgtagcgg gtgatggagg aaccgatcgt gaccgagtcc atctcgacgt
    22621 cgatgttgaa cttcttcagc gtacggcgga tggtgttcga gctcgccttc acgtcgccga
    22681 cgtccggcgt acccttgtcc ttgtggagga gggagagggg aggaggcgcg tacgtgccct
    22741 caaagctcga gacgacgaac tcaccggccg ccttcgagag gaacttctcg gtgagcgaac
    22801 gcttcttcgg cttctcatcg tcggcgttcg cctcggcggc cgccgcggcg ccttccagag
    22861 gctcaccgga atccggtagc ggcatcgtga cgacgggctc gagcgcctcg tctacttcct
    22921 cgtcgcgctt cctatggagg aagtccatga agctcgtatt gaacgtgagg aagagcgcga
    22981 tgaggaggat agcgacgagc atgatacccg cgacgatacc gccgaagagc gacgcgaacg
    23041 gatagagcat gagcgcgccg gcgccgccgc cttccgtaaa ccagagttcc atgagtccga
    23101 gagaggcgag gaagaagagc gtgacgccga cgagcttgga gacggagacc ggattgtctt
    23161 cgagcggctt cataaagacg tatacgaaga ggagacaggc gacgggcgcg aggacgaagc
    23221 cccagccgaa gaggtaagtg agcgccctga aggtcatgtc gcccgcgatg cccgcgtagc
    23281 cgagggacga gaggacgaag aagaccgcga ggacgacgaa gaagatggcg gctatcgcgt
    23341 gcttcgtctg gggcgagacg tcatgaaaga aggaagggcc tgcgggcggt ggcgtcgtcg
    23401 gtctgcgtcg tttggccatg cttagatttt attatgaatg taatgaatta taaaatctta
    23461 gccgtcgccg agtgcgtctc tccttgacgc ctcgcggcga cgaacgagca tttgactaaa
    23521 actcgcacgc tctatgacag atttgtaacc tctgcatgat agcacacggc gactccgaga
    23581 aaccgtgtgg attagcggat ccatgcgtac atgaggagag acgaacagcg ttgcgtatgc
    23641 gacaaaaagg cgtatactga ccgatacgaa tgtccgctat tataaaggtc aggtatacga
    23701 catcatggaa gccaaagaga cagtattaga gacagaacgt cgacatcgta tcgtcaatga
    23761 taccgaatat ttcaaatata tttttaaaaa gacagaaaag attgcgtgtg ctgtcgttta
    23821 tgtgctgcgt ggtgatactt caatccgtca taccgaccag attgtcctag acctcgaaac
    23881 cgcatctcgg acagttctcg atgcgtccct tgcgtctctt aagagcaccg acctcacggt
    23941 ccatgagagc gcgcttagtc tcgcgtacga gctcatgaac ctcgagtcga agctccgggt
    24001 cgcgcacgcg gcgcggctca tcgaccaggg ccttctcgat gtcttcgtgc atgagatcga
    24061 ctcggtccat cgtgcgctcc gcaagtacgt gggcacggac gttcgaaacc cgcttcttgg
    24121 cgatacggca cccgagagga gggagcgcga ggggagaacg ccgcgccgta gcctcgcgcc
    24181 cgtgggcgac gcgcccctta cggagcgagc accatcgccc tcgagcgtgg ggaggcgcga
    24241 ccgcgtcctc gacgtcatcc gtactaaggg acaggcgagt atcaaagaca tagtagaggt
    24301 cgtatccgac gtgagcgaaa agacaatcca gcgagagctc aatagtctta taaaagacaa
    24361 tatagtacat cgcgagggag agagaaggtg gagcaaatac agcctggttt agacaaagac
    24421 aattctctca agagaaaaag gacataacgt gtggcgttat gtcctttctt gttataaaag
    24481 acgttatcat ttgtccatat cttcctcatt ttatcatcgc aggacgatgt cctaaaggac
    24541 gcagtgtttc cgccctttaa gggcatttcg gtgcgtaaga tgttcgcgca tagtgcgtca
    24601 tcaccggtag cgagagcgcg ttcgtgttga ctttatgaag acgtgcacgt atgctctcat
    24661 ccagattttg tggcttgatt cgtaggcagt tatgcacaca tcgccgcact atccggggta
    24721 tacaagcgaa acgcttttgt atactattcg tatgcgcttc agtgcgctct tttttgaaag
    24781 gaaaagggac cacgttttgg agggtagctc ggttcatcca agtatccacc aaatggcgcg
    24841 tatactcgtt ctttgagaga cgaacacgcc atgtacatat gtatgtacat ccgtttttca
    24901 tgagacatat gaaaaaatgg tggaggcgag gttcggtccc gggggaaaca cctggatctc
    24961 atatctaagt ccgcagatgc ggaccgacgg cgtctacgac acagacgcac tttgccgttt
    25021 cctgttcggg aacggtgctt cagaaattcc ttttcccgtt acggttgcgt acgatagaaa
    25081 ttccttctct cttgtagcta ggccacggcg cgtggggtgt cgacccgtta acgcgcgccg
    25141 cggaatagtg atattcgtga acggtccttc gacgggccgt tcgcaaataa gaattaattc
    25201 ttattattag gttaacagta attcgttact ggtaaacagt tatgaacatc acagcaaaca
    25261 atgctgtctc gaagctcttc gtagcgttcg tggcagcagc gatgctcttc atgctcgcgg
    25321 ctccttcagc gaaggcggct acggttgagg agcttcaggc acagatcgca gcgctcatgg
    25381 cgcagatcgc agccctctcg ggcggatcga cgtcgggcag cacggcggct tgcaccttta
    25441 ctcgttcact cacggtgggt gtctctggcg acgacgttaa gtgcctccag aactacctca
    25501 ccccgaccta cttcagcaat gcggcaggcg caacgggcta cttcggtccg gtgacgcagg
    25561 cggcggtcgc tgcatggcag gcggctaacg gcgtcacgcc ggcggctggt tacttcggtc
    25621 ctgtttcaca ggctaagtac tccgctatgg cgtcggcagg tggcaacacc ggctcgactg
    25681 gtggcaacac cggctcgact ggcggcaacc agggcggtag cacctcgaac ctttcgggcg
    25741 aggctgacct cgatgacgtt cagatcgacg atgctgacga cgatacggtc caggagggcc
    25801 aggacgacgt tcagatcggc cagattaccg ttaagttcaa gaacggcgat gctcgcatcg
    25861 atcgaatgga catcgctctt cagggcggtg acgatgagac cgatccgtgg gatacgttcg
    25921 acaaggtcat gctctccgtc gatggcgatg agatcgcttc gatggacgct tctgaccgag
    25981 acaactacct taacgatgat gagggctcac ttcgcttctc gcatctcgat ctcgttgcaa
    26041 acgaggacga tgaggtggtc atcgacgtaa cggctgacct ccagtccggc atggatgatt
    26101 ccaatgacgg cgaggcgtgg tcgctctacg tcggttctgt ccgctacttc gatgcggagg
    26161 atgttgcgac gaccgaggac ggagttggtg accaggtttc tgatttcggc agcgttggag
    26221 ctgcggcatc ggctgacttc accattaacg aggcgggtaa cgatgacgag atcatcgtga
    26281 agacctcgtc ggaggatccg gacgctacga cgctcgaggt caaggatgac cagaagtctg
    26341 actggtacac ggtcttcgcg ttcgacctcg acacgaagga ttccgagaac gacattgacc
    26401 tcaacaccat ccaggtgggc gttcacacgc cgggcacgtc gacctacaac ggtctcgtgg
    26461 acgacgctga actcgttatt gatggcacga ccatcgatga tgtgacggtc actggcggtg
    26521 gtacgaacga cgcaacgctc agtttcgacg tcaacaacga tgtgacgatc aacgctggcg
    26581 accgcgttac cgcagagctc aagctcaagt tcaagtcgct cgcttcgggt gatgagggcg
    26641 caacgatcta cgcagaggtc agctcggctg atcgcgcagc gatcgacgcg acgggtgcag
    26701 atgatctcgc agcaggccag ctttctggct cggcgacggg tgatacgcac acgctccgca
    26761 cgtcgggtgt agcggtcgag gatttctcgg cagacaccga cgtaacgacc tctgatacgg
    26821 caacgaacag ctacggaacc ttcaccttca ccttcgacgt tacgacgatc ggagatgacg
    26881 aggtgttcat cccgcttacg gctgcttcga cgaccgcatc gggctcgacg acgaagggtg
    26941 cagtctacaa gatcactgac gctaacggca cgaccgttac ggcatctgac tctaactcgg
    27001 cttcactcgt tcgtatctcg ggtggcgaca ccagcacggc tggctatgtc cgcctcgatt
    27061 ctggtgagac ggcgaccctc aagttgacgg tcacgttcaa cccgacgacg gctggccagt
    27121 tcatcggtgt actcgacagc gttgggttcg cggctacggc tgatcctgct acggacagca
    27181 cgacccttac cccgtcgacc gactacgata ctgacccggc gttcatcacg agctaattcg
    27241 cacgaattag cctagatgat ccgttcggat ctagtctagt cagcaaaaac tccccgcaag
    27301 gggagttttt gcgtatatgg attcggaaag agaagaaggg aaacgaaaaa tgcacgcgca
    27361 tcgcgcgtgc atttttcgtt tccctggtat actaacctca tgttcaacag gtccttctac
    27421 cgcttcctct tcggcttcct cgccatcatc gcgtcgaccc tcattctcat cctcctcgtc
    27481 ggcgggagca agggttcata atcgccatcg accatgagaa tcaccgatca atacttctgg
    27541 aacgtcatct ttctcatctt cttcgtcgtc ctcgtcttcc tcgggacggt gatccttgag
    27601 agcgaggcgt ggaagccgct ttccgacctc accatgacgg actacgtcct catcacgctc
    27661 gcgtcgtttc gcatcatccg cgccgtcctc tacgataaga tattcgcgtt cttccgcgag
    27721 cagttctacg acgcgaagga ggtgcgcggg aaggtcatac tcgtgaagcc ggagagtggg
    27781 ccgcgccgca cgctcgccga gctcctctcg tgcccgtggt gcttcggcat ttggtcggcc
    27841 gcgatggtct cgttcttcta tctcttgagc ccggtcttct tctatcccgt cctcttcatc
    27901 gcgctcgccg gcgtcgcctc gtttctccag atactttcga attacatcgg ctggaaggcc
    27961 gaagaggcga agcagaacgt cgaaggacga atgtagcggg gaagggcatg gagagaggaa
    28021 gcggccgcga ggccgcttcc ttttttcgcg cactctggta tagtgtccgg gtcagactct
    28081 agacagccgg tcgctttccg aaaaggggaa gccataatga gccgtttggc tcgccagccg
    28141 aggtccaact actcctagta tttggcggtc gaactctggt ctctcccatg gggaggtctg
    28201 acgtcattag caatcagtac gtttcggtct actgtggcaa taacgaaagc aaagaagcag
    28261 gatatcctcg cgaagctcga gaatatcaag aaggacgccg aagcgatcgt cttcgtgaac
    28321 ttcaagggtg tcacggtgaa ggatacgacg gccatgcgcc gcaccatgcg ggacgcgggt
    28381 gtcggttact tcgtcgcgaa gaagaccctc atgaagcgcg cgttcggcga ggcgtacgag
    28441 ggcacgatgc ccgagctcga tggagagata gcggtcgcgt attcgaccga cgccatcctc
    28501 ccggcacaga acgtcaaaga cttcgcgaag cagttcaagg acaagatcgc gatagccggc
    28561 ggcatgttcc agggcgtctg gaagtcgaag gaagagatga cggagatcgc gtcgataccg
    28621 tctctcgacg tcttgcgcgg tatgttcgca cagctcatca actcgccgcg tcagcggttc
    28681 gcggtggtgc ttagtaaggt ttcggaaacg aagtcctaat ctattaacga ctaactatca
    28741 gtattgtgaa taaggatcaa atcatcgaag cagtggagaa gatgaccgtt ctcgagctca
    28801 acgatctcgt gaagaccatc gaggagaagt ggggcgtgtc cgcggcggct gtcgcggtag
    28861 cggctccggg tgcggcggct gacgctggcg aggagcagag ctccttcaac gtcgagctca
    28921 ccgacggcgg cgcgcagaag atcgccgtca tcaaggtcgt gaaggaggct ctcggcctcg
    28981 gcctcaagga ggcgaaggac ctcgtcgatg gcgcaccggc tatcgtcaag gagggcatga
    29041 agaaggagga tgctgaagcg ctcaaggcga aactcgagga agctggagcg aaagttacgc
    29101 tcaagtaaat tttctcacaa aacaaaaaca ccccaatagg ggtgtttttg ttttgcaaaa
    29161 aaatatgagc gtaacttttg ctccgagcgt agcgaaatag tatgtctcgc aactagcggt
    29221 aaagtaacac gtaagtaaaa agaaacccca cgctatcgcg tggggcgcgg ggtcgactcg
    29281 agctcgagaa gacgctcgat ggaaacccgc atgtagttac cgaggtcatc tttcgtgtag
    29341 aggtggaacg aaagcgcgtc ggagacggcg agccccttga ggaaccgctt cgcggcgcct
    29401 ctcgtcgttc gccagaacac gtggtcgacg aggtcgaggc ggacgtgaga gctcaagaga
    29461 cgtttttctt ggagccgtcg ttcgagccgc ttttccaccg gctctggcag ttgctttatc
    29521 tccagccaag cgggtgcgtt ggggtcatcg ttttcctccg gcatccgctt ctttcgcgtc
    29581 ctcatgcgcc ctccctcacg gtcggccgcg tccgcttgag ggcgcctttc gcccgtcttg
    29641 tcttcgcgcc caccttcttc ccgtcggagc ggtaacggta gagcggtccg ccctccgtca
    29701 ttcgcacgaa gagcttgtag tgcccgctga agctcggctc ggacgcgacc cgcgcgatga
    29761 ccgccgaatc gacgcggtag agctgctgtt cgtccgcata tccggggacg cggcgcgtca
    29821 cagtttgagg gagcttcacg tcgatcgtgc cgagcgcgac cgcctcgctc acgaggttcg
    29881 agagcgggag gaggtagtac tcgaggaccg actgcgccgg cctcgccttg aagccaggac
    29941 ggaacgtcgc cgcgatggcg cccatacgca caacggtctt cctcgtcatg attcgtcctt
    30001 tcggtttgaa atgtacggcc gcgctcatag tatcgaaaaa cggggaatga gcaaggcagt
    30061 gaaaaacgct ccgggcaaag ccggagcgtc gtggtcgttt aggtcgtcgg ttgcggacgg
    30121 cggtacgcga agaagaggca cgccgtgccg gccgcggaaa ggacggtggg aagcacaccg
    30181 cccaggatgc cctcgatggg cgggcgggga agcgttcccg agagggcgag ccccatcagg
    30241 acgccgccga tgacgaggag ggtcccggcg atgccgaggg tgaccttcat atcaacgctc
    30301 atgcgggtgg ccatgcgttc tcccgtgtcg tggaaaccgg aaaaacacta ccacgaatct
    30361 cgaatgcgtc aatggcggaa tggaacactc gccgctttgc ctgtctagac atgtggataa
    30421 tgagccgcgt gctataatcg cggcaacgga attcctccca tgaaagactc tctcgccatt
    30481 ctcttcggta accaggcacg cgtcaaactt ctccgattct tcctcttcaa tccgaagcag
    30541 gaattcacgt tcgatgatat ctcgaggcga gcgaaactcg tccgtcgcac ggcacgcacc
    30601 gagataaacg cgctcgagcg cgcggaggtc atcaagcgaa agatgatata cgtgaagcat
    30661 ccgacgaagg acgcgaagct tcgcgtgcaa ggctacgtgc tcaacgacaa atttccccag
    30721 ctcaattcgc tccagtcgtt cttcttcgag acggcgccca tcaacggtac gacgctcctc
    30781 aagcatcttc gaaaggcggg accgctcgac tttctcgcgt cgacgggcgt cttcatgcgt
    30841 gagttcgacc gccgcctcga tttcctcgtc gcgacgaaga aattgaacga gcaccgggtc
    30901 gagatcgcgg tccgagcgct cgaggcggag cttggcatcg agatacgcta cgcgacgttc
    30961 tcgacggagg acctcatcta ccgcgtcggt atgtacgaca agctcacgcg cgacgtcttc
    31021 gattacccgc accagatact cgtcgaccgc gtgggcgtcc gaaacgagct cttgagccgc
    31081 cccgccttcg cctagagagg gaaggaaaga gtccccgtga ggggcttttt tcgtccgttt
    31141 ttcgcgaaac ggagccgaca cctgaaatag cgcttgactc tcgaatcctt tcgtatataa
    31201 tggcgcctac gtgtttatga gaatcactaa ggaaaccacc tagcggacca tcttcgccta
    31261 gcgaagtgtg tgccgcggag agcggctttt ttagtcaaaa agttcaggga tggacttcgg
    31321 agagattctc acgggaacat gcgatcgttg aaaattgcat attccggaaa cgagattgat
    31381 aaggtctcgt ttctaaaatc ctacgtgttc gacgcacgta ggtgtgttcg ctattcgaga
    31441 gaatagcgga cgccaggtaa gcgcatgcag cgaagaggac aatgcttcgg cagggtcttt
    31501 tcgtccgctg catgcacgac acaaagtctc acctgagctt gtatctcagg tgagcgcact
    31561 caaaaattgt acagcggtgt acagcgttcg tgtaatagac tatcgcggca caaaagatgc
    31621 cgtgtccagc cttccttaca ggaacgataa gggtgtatgg tggatgcctt ggcttcaaag
    31681 agcgatgaag gacgtggtat ggctgcgata agcgcggggg agatgccaaa cgatcgtaat
    31741 atcccgcgat gtccgaatga ggaaactcac ctgcttacgg caggtaccct cgcaagaggg
    31801 agcgcacccg gtgaagtgaa acatcacagt agccggagga agagaaagca acacagggag
    31861 gtttctttct gatcggaggg ccgcaaggct caccattacg gtcaggaaga agcttcttgc
    31921 gatcccctaa gtagcggcga gcgaaaaggg aagagcccaa accattcgtg tttacacgaa
    31981 tggggttgta gggcaggagc gtcatttagt tgagaagagt caccaattgg tgtgatagca
    32041 gaacgcgttg ggaagcgcga ccctagcagg taatagtcct gtatgcgaaa ttacatcaac
    32101 tcttttgctt ctgttcctga gtagttcgag acatgaatag ctcgaatgaa tctgggagca
    32161 ctaactccta aggctaaata tctttgaaga ccgatagtga actagtaccg tgagggaaag
    32221 gtgaaaagaa ccccggagag gggagtgaaa tagaactgaa accatacatc tacaaggagt
    32281 cggagccctg cggggcgacg gcgtgcctat tgaagaatga gccggcgagt gtatctgtat
    32341 cctttgtcta agcggtaacc ccgcggaggc acagtgaaag cgagcgtgaa tagcgcgaat
    32401 atagggtacg gatacgaccc gaagcgggac gagcttgcca tgagcagggt gaagcggggg
    32461 taaaaccccg tggaggcccg aaccggtgga ccgttcaaaa tcctcggatg acttgtggta
    32521 gggagtgaaa agctaatcga gttccgtaat agctggttct ctccgaaaca gctttagggt
    32581 tggcgtcgcg tgttcctcct gggggtagag cactggaagg tctaaatagg gggaaacccc
    32641 gttagaccta tcaaactacg aataccaggc agttagagcg cggcagttag actacggggg
    32701 agaagctccg ttggtcaaaa gggaaacagc ccagatcgcc agttaaggtc cctaaatata
    32761 tgctcagtgc gtcttgagga ggtgaggatt cactaacagt caggatgttg gcttagaagc
    32821 agccatcatt taaagaaagc gtaacagctc actgatcgag agtcctcgcg ccgaagatga
    32881 tcggggctta agcatattac cgaaactgcg gattgtacgt ttcttcggaa acgtacagtg
    32941 gtaggagagc gttctctgtt gcgatgaagg gaaggtgaga accgccctgg agtacagaga
    33001 agtgagaatg ccggcacaag taaccgcaag atgggtgaga accccatccg ccgaaatacc
    33061 aaggtttcct tcgctatgct gatcagcgaa gggttagtcg gtcctaagcc gatcccgaca
    33121 gggggaggcg atggatagca ggttaatatt cctgcacact gttctgttcg aaggagggac
    33181 gaagcgttgt actcgaagca tcttattgga ttggtgctgg agctacgagg aggtctggta
    33241 ggcaaatccg ccggacatta actccgagta gagtctgaac ctaaggctac ggccgaggga
    33301 acgagaggag cacgctttcg agaaaaactt ctaaggttaa gcaggacggt tccgtaccgc
    33361 aaaccgacac aggtggtagg gtcgagaaga ccaaggcgaa cgagtgaggg gtccttaagg
    33421 aactcggcaa aaaagcggcc gtaacttcgg gataaggcct gccctttctc cgcaaggagg
    33481 cggggccgca gcgaaagatt tcctggcgac tgtttaacaa aaacacagct ctctgcgaag
    33541 acgtaagtca atgtatagag ggtgacgcct gaccaatgcc ggaaggtcaa acatggaagg
    33601 tggtacggat tcgtccgtac tgctgggcta tgtaagcccc ggtcaatgtc agcgataact
    33661 ataatcgttc taaggtaccg cgcgcgtcca cgacgcgcgc ggtaggactg gaacgattat
    33721 gggagtgtaa aacagatacg cataaagcta cctttgcgcg cagcgatgca cgcattgtcc
    33781 aaatgtgacc atcaagagac caaatgtcac acacctctct gaggtgaaga tagagtcctt
    33841 cttgcaaaag aaaaaaggaa cagaagagcg cagttccttg tcgggtaagt tccgacgtgc
    33901 acgaaaggcg taacgactgg gaaactgtct caaggacctg ctcggtgaaa atacaatggg
    33961 ggtgaagatg ccccctacct gcaattagac gaaaagaccc caggagcttt actgtagctt
    34021 cgtattgagg ctgtttgatt gatgcgtagc atagatggga gaggttgaag cgttcctttc
    34081 gggggacgtg gactcgtcag tgaaatacca ttcttcaatt gggcagtttc taacctcgtt
    34141 ggcaaaacct tcgagagaca gtgcgtggca ggcagtttta gtggggcgct accctcctaa
    34201 agagtaacgg aggggtctca aggttggcta ggtgcgaatg gaaaccgtac cgatagtgta
    34261 atggcataag ccagcttgac tgtaaggtgt cagtaccgaa cagatgcgaa agcagagcat
    34321 agtgaaccga tggtccgcgt tagatgcggc cagagattat ctgacaaaag ttaccctggg
    34381 gataacaggc tagtcgtgcc caagcgtcca tagcgacggc acggttcggc acctcgatgt
    34441 cggctcaact tatcctgggg ctggagcagg tcccaagggt ttggctgttc gccaattaaa
    34501 aagttacgtg agctgggttt agaccgtcgt gagacaggtt ggtctctatc tattgcaggc
    34561 gtcgattctt gaagagattt gtccctagta cgagaggacc gggatgaact gacctctggt
    34621 ctatctgctg tcacgccagt ggcaccgcag agtagctatg tcgggaacgg ataactgctg
    34681 aaagcatcta agcaggaagc cgactctaag atgaggaatc attaagtccc ctgagagatg
    34741 atcaggttga taggcatcag atggaagcac agtaatgtgt tgagtcgaga tgtactaata
    34801 ggacgtagtt cctgtaggga atgctgcaca tggcacacgc gccgtgtacg caagaactcc
    34861 gattcggaca tacgttcgaa ttcgaaagca accgtcgcac gcgctcggtt ggagggaatg
    34921 ctgcacatgg cgatacgcga tggaggcatg aacgctggac tgctgacaat ttttgaagtg
    34981 tttgtacctg atacggaata tgcgatgtcg agggtgtggc gcagctggta gcgcgtcggc
    35041 cgtgaggccg aaggtcgttg gttcgaatcc aaccaccccg acccgacccc gcggacggac
    35101 gaaattcggt ccgtccgcgg aggcacgtgt tcattcgaga attgaatatt taaagttggt
    35161 gctatagcga gaagggtgag acagtccggg ggactgtcgc aagaccttga gcagttgctg
    35221 cgaaaggtgt acagctcctg taaggagcac cgacgaacgt agattgaaaa ttgaatattt
    35281 aaagttggtg ctttcgcgag aagggtccac ccgttcccat tccgaacacg gaagttaagc
    35341 ttctctgcac cgatggtact ttcttggaag ggagagtagg tcggcgccaa ctttaagtat
    35401 tcaaaagaaa aaactacccg tttgggtagt tttttctttt gatgtcccta aagtgacgaa
    35461 ttagttttgt cgacttcgtc cagcggtttc ctctggtaga ggaaactgag acagaaattc
    35521 agcatagtcg cttcgctcgt tgcttcattt cactttaagt attctgtggc tttcatcccc
    35581 gtgctcgctc gtctcagtag gtcggcgcgc gcatattttg tatttcatgc aaatacgata
    35641 tagtggcgaa atgcgacaga ccataaccga cccgggggcg tcagagctcg tctacgagat
    35701 acgcgagatc gtcgaagtgg cgaagaagtt cgaggccttg ggtcagccga tcacctggga
    35761 gaacatcggc gacccggtcg cgaaagggga ggaggtcgcc ccgtggatga aggagtacgt
    35821 cgtcgaggcg gtcaaggaga atgccgtcta cgcgtactcg ccgacgaagg ggcttcaggc
    35881 gactcgtgag tacatcgcga acgagcggaa cctcgagggc ggcatcgaga taacccccga
    35941 cgacatcctc ttcgtgaacg gtctcggtga cgcgatctcc aaggtgtatc ggagcctcaa
    36001 tccgaaggtg cgcgtcatcg ggccgaatcc cgcgtatccg acgcattcct cggccgaagc
    36061 ggcgcacgcc gacggccctc acctcacgta ccccttggac cctaaaaacg gttggaagcc
    36121 cgacctcgat gcgatggagc ggaccatcgt cgaacacccg gagatcggcg gcattctcat
    36181 cgtgaacccg gacaatccga ccggctacgt ctacccgcaa gacgtcgtcc gcgatatcgt
    36241 ggcgatcgcg aagaagcatg acctcttcat catctccgat gagatatact cgaacctcgc
    36301 gtacggcata gagcacaaga agctcgcatc ggtcatcgac gacgtccccg gtatcgcgat
    36361 gcgtggcgtc tcgaaggagt tcccctggcc gggcggacgc tgtggctgga tggagttcta
    36421 caaccgcgac aaggacgagg acttcgaccg tttttgtaag tccctgatcg acgcgaagat
    36481 gctcgaggtc tgctcgacga ccctcccgca gtacgtgctc ccgcgcatca tgaaggacga
    36541 gcgctactac ccgatgctca aagagcgcac ggcgcggtac gcgaagcgag cgaagctcgc
    36601 gaaggacgta ctctcggaga tccccggcgt catcatccat gagcccaacg gcgcgttcta
    36661 tatgtccctt gtcttcgagg cgggcgtcct cactgaccga cagacccttc cggtagagaa
    36721 cgaggccatt gccaccgagc tcggaaagct cctcaccggc gtcacgaacg acaagcgcct
    36781 cgtgtactac cttctcgcgg cgaccggtat ctgcgtcgtt cctctcacgt ccggattcaa
    36841 ctcgacgtac gatggtttcc gcttcacgct cctcgagcga gacgacgcga agtttcgaga
    36901 gatcatcgag accctccgcg atgcgatacg cgcgtacatc ggctcttgat atcgaaagag
    36961 ccggcctaac cgcatctgtt acaatacctc catgagatgg tgggcctttt ggcgtcgagt
    37021 cgagtacggg gccgcgttcc tcgtcctctg tgccggtatc ggtacgggcc tctattatat
    37081 gtacgggtat gaggcgccga cgtgcttcga cggcctccaa aacggcgacg aacggaacgt
    37141 cgactgcggc ggcgtctgcc agcgtatttg tcccatggat atcacgatgc cgaccgcgct
    37201 ctgggcggaa tcgttcaaag tcgtcaatgg acagtacaac gctgtcgcat acgtcgagaa
    37261 tcgaaacaaa gatatcgggt ctccggagct ttcgtacacg ttcaagctct tcgacgcgca
    37321 aggagcgctc atcaccgagc gctcggggac gacggtcctt ccgcccgatg gcgtctatcc
    37381 tatctttgag ggccgcatca tgacgggcga ccgcgtgccc gcgtacacgt cgatcgagtt
    37441 cggcgaggac atactctggc gaaaggggag cgtcgggcgc gaccagttta cgctcgagaa
    37501 gcgcgagctc gtaaacgccg atagccttcc caagctcacg gcgaacctac ggaactcgtc
    37561 gctcgacgaa gcagaggacg tcgagatcgt cgcgacaata ttcgattcac agaagcaccc
    37621 cctcaccgcc gcgcggaccg tcctcaagta tttcccaggg cggacgagcg aagacgtcgt
    37681 cttcacgtgg ccggaaccca tcgcgaagac cgtgaagagt tgtgagatac cgaccgacgt
    37741 cgcgctcgcc atcgacctct cgggaagcat ggacaacgat gggggtacgc cgcccgaacc
    37801 gataagctcc gtcctcgccg cggcgaccga tttcgcgaag cagttgaaag aggacgacca
    37861 ggtgaccctc gtcacgtacg cgacgaaggc cgaggtcgtc gaacccctca cgcacgagtc
    37921 gtcgcgggtc gcgaacgata tttcgaaact cgccatcgac ccgaaggagc agaccgggtc
    37981 gacgaacacg ggcgatgcgc tccttaagat agcgagcgag ctcggttcga cccggcacag
    38041 cgccgacgcg cggaaggtcg cgatcctcct caccgacggg ctcgcgacgg cgcccgacga
    38101 tcccgaggtc tacgcgatac gggaagccga ggacttgaag cagatggacg ttcttctctt
    38161 caccatcggg ctcggcgccg acgtgaacga ggacttcctc cgcgccatcg cttcgaatcc
    38221 atcgatgtat cacaaggcgc catcggcgaa ggacctttcg tcgatatacg ccggcatcaa
    38281 gaaggacatc tgcgaggacg gcccggccgt catcgagata atcgcgaagc cgaagacctc
    38341 gttccagtag ttttgaccgg catacgcttc tcgcgcataa tggactcgtg acgctcgtcc
    38401 gaacactctt caaaagcatc gattggtggc ttatttcagc cgtacttttc cttacctttc
    38461 tcgggctcgt cacgatgtac acgttcgacg gcgataactc gtacttcacg aagcagatcg
    38521 cctggatagt catcgcgacc gcggggctcg ccatcgcgat cgttccggac taccggttcc
    38581 tccgcacggg gaacacgacc ttcttcatct acctcggcgt cctcgtcctc ctcgtcctcg
    38641 tcctcttcgt cggttcgacg gtgaagggag cggagagccg cttcgacctc ggcgtgttct
    38701 cgctccagcc gtcggacccc gcgaagctcg cgctcatcgc cgtcctcgcg aagtacttca
    38761 gtaagcggca tgagctcatc ggcgactttc ggcatatcct cgtctccgga acgtacgcgc
    38821 tcctcatctt cggactcgtc ttcatccagc cggacttcgg gtcggcgatc atcctcttct
    38881 ccatttggtt cggcatggtc ctcgtcgcgg gcatcaagtt tcgtcacctc ctcatcgtct
    38941 ttttgatagg cgtcctctcg ttcggcgtca tgtggcagtt cattttcctc gactatcaga
    39001 aacagcgcat cctcacgttc ctccatccct tggcggacat acaggggacg gggtacaacg
    39061 cgtaccagtc gaccatcgcg gtcggctcgg gtcaacttct tgggaaggga gtcggctacg
    39121 gaacgcagtc gaagctccag tttcttccgg agtacgagac ggacttcatc ttcgcggcgt
    39181 tcgcggagga gtgggggctc ctcggcgtcc tcctcctttt cttcctcttc ggcgtcgtca
    39241 tctggcgtct tctcacgcac gcggtccatg gcgcgacgaa cttcgagcgc ctcttcgccg
    39301 tgggcgtcgc cattctcatc atcagccact tcttcgtcca catcggcatg aacatcggtc
    39361 tcctcccggt cacggggacg accgtcccgt tcctctccta cggcgggtcg cacctcctca
    39421 ccgagttcct cggcgtcggc atggtcatcg cgatgggccg ctatacgaaa cgtcactatg
    39481 tcggcgagga cctcctcgcg agctaatctc cgtagagcgc gaacgtccgc tcgagcattc
    39541 gtgcgatatc gaattcacgc gcgaccttct ctttaagcgc gttgccgcaa gcgtcgcgct
    39601 tttccatcga gcgcgcgtac gtctcgaggg cggtgacgat ggtgtcggta ttccgagggt
    39661 cgatgaggag gccggtcgcg ccgttctcga tgacctccgg tatgcctccc acgtcgctcg
    39721 cgatcgaggg aaggccggcg gcacccgctt cgaggagcgc gtacggcata ccctccttaa
    39781 gggaggggag gaggaagacg tcgaaggccc ggaggtaggt tcgcgcatcg tcgagatatc
    39841 ccgtgaaccg gaccgcatgc gagaggccgc ggtcgcgtac gtacatctcg agctcgtgac
    39901 gcagttcgcc gtcgcttatg agcgtgtaga agatcttcgt gtcgcgcacc tcgttatagc
    39961 gcgagacggc gtcgagcgcg gtgaagaggt tcttgttctt cgtcagttcc gcgttcgtga
    40021 cgagccagag gtcgtgctcg tgctcggata cgacgcccgc cgagaagagg cggccgcgcg
    40081 cgtccgcgcg gtcgagaaaa tcgatgtgcg gcaccgcggt atgtatgagg gtgagcttct
    40141 cgcgtgagaa ggggagtacg accgcgcgat cgcgcgcgga gacgacgatg acgcgatggg
    40201 agaggagcgc ggtgagccat gagaagaacc acacgaccgc tctccagaga gctccccgat
    40261 cctcgtagaa cggccagccg tgcgcggtga agatgatctg acggacgcca aggagtcggg
    40321 cgacgaaggc gcccgaaccg cccgccttcg aactgttgag atggacgacg tccggtcgct
    40381 cgcgtcgaat gattcgagcg agctcgaaca tcgatcggag ttccttcgtg atgctgatgt
    40441 cccgttcgaa actcgctatc gtgaccgtgc gaacgcccgc ggcgcgcaac ttctcgaaga
    40501 ggatgccgtc gccgcccgcc gcgacgacgg gctcgaagcg gtccctggga agcccggtcg
    40561 cgagttcgta gacgtaccgc tgagcgccac cgaaattcga cttcgttatg atgtacagtg
    40621 ttttttgttt ttttgcgttc gtcgtcatag tgttccttga gggtggcgca caactgggga
    40681 gtgccccgca ctccccagca cctcaattcg acttcgttat gatgtacagt gttttttgtt
    40741 tttttgcgtt cgtcgtcata gtgttccttt tcgagctcgt cgtcatggcg tcttccgtac
    40801 tcgtcgtttc tataacttaa gaacccacct ccctggtacg acggcgaccg cttgcgtaaa
    40861 cgcgcccttc tcgtattata ccttccatgc acatgttcgt gcgcggtagt accgtgctac
    40921 tcctcatcgg cgacctcatc gtcttcacgg gctctctcgt ccttacgctc ttcgtgcgct
    40981 accaagcgtg gccgtcgcac gacctcgttt cgacgcacct gcgcgcgttc accgttctct
    41041 tcgtcttctg ggtcctcacg tttctcatca ccggcctcta cgaccggtac atctcgctcg
    41101 ttcgaaagag catccccgcc ctcgtcctca aggtccagtt cttcaacatc ctcctcgccg
    41161 cgctcatctt cttcatcttc ccgttcggca tcgaaccgaa gacgaacctc gtcatctacc
    41221 ttctcatatc gacgctcctc atcgtcctct ggaggctcta cgtgtaccct ctgatcgcga
    41281 cggggaagcc gatgcgcgcg ctcgtggtcg gcgatagcga tgaggccttg gccatcgcgc
    41341 gcatgttcca ggggaacccg ttcttcagga acatcaagcc gtttctcctt cgaaagaagg
    41401 acatctcgga cttcgatgag ttccgtgcgt cgctccttcg cttcgtcgat cagggctccg
    41461 cggacatggt catcgccgac atgcgcgacg agttcgcgaa tcgtcttacg cgcgacttct
    41521 ataagctcgc gttcgagaac tcgaacatca ggttcttcaa tctgccggcg atctacgaac
    41581 acctctacca tcgcgtgccg ccggagctcg tcgaggagag ctggctcctc gagaacgtct
    41641 cggccggttc gcctcactat gcgtacgatg tgctgaagcg cggcatcgat ctcgcgggcg
    41701 ccatcgccct cctcgtcccg gctctcgtcc tctttccggt catcatgctc gcgatctacc
    41761 tcgaagaccg cggggcgttc ttctacgagg ccgagcgtgt cgggcagttc aatcgaccga
    41821 tacgcattct caagttccgg acgatgaccg gccgcgacgc ctcgggcgat gcgctaaaga
    41881 gcacgcttac gatcacgaag gtcggtacgt tcctccggaa gacgcgcctc gacgaactgc
    41941 cgcaactcct caacatactc atgggagacc tctccttcat cgggccgcgc ccggagatac
    42001 cgtccctcgt ttcggtgtac gcggagaaca tcccgtacta caacatgcgg cacctcacga
    42061 agcccggtct ctccggttgg gcgcagataa acaacttcga cgtaccgcga ggcggcgtcg
    42121 acattccgcg aacgatcgac aagctctcgt tcgacctcta ctacctcaag caccgctcgc
    42181 tcatcctcga catcgagata gcgctgaaga ccataaatac gctcgtcctc cggagcggaa
    42241 cgtaacggag gagggatttg ttacaatcgc tgaatactcg ttaaccacag accaatgtcc
    42301 acgtacctca tcaccggcgg cgccggcttt atcgggacca atctcgccga gcgactcgtc
    42361 cgtgacggac agacggtggt cgtcgttgac gacctctcgg cggggaaggc ggagcgcctt
    42421 cccaaggagg tcgatttccg gaagctcgac atctgcgaca ccgcgacact tacggcggtg
    42481 atgaagggcg tcgacgttgt cgtccacctt gcggcgctcc cgcgcgtcca gttctcgatc
    42541 gaacatccct tcgaggccca gcacgtgaac gtcgacggaa cgctctccgt cctcgaggcg
    42601 gcgcggcagg cgggtgtgaa gcgcgtcgtc tatgccgcgt cgagctcggc ctacggtgac
    42661 caagagaacc tcccgctctc cgaggacctt ccgccccagc cgaagagccc gtatggactc
    42721 cacaagtact acggcgaggt tctcatgaaa ctttggcatg acattcacgg tctcgagacc
    42781 gtctccctcc gcttcttcaa cgtctacggt cctcatttcg atcccgaggg cgcgtacgct
    42841 ttggtcatcg gaaaattcct caaacagctc aaggaaggca agccgatgac ggtgacggga
    42901 gacggcgagc agtcgcgcga cttcacgcac gtcttcgata cggtggacgc ggtcgtacgc
    42961 gccaccgtga gcgagcaggt cgggaagggc gaggtcataa acgtcggcgc gggacggaac
    43021 gtttccatca acgagctcac gaagatgatc ggcggaccgg tcgagtacgt cgcaccgcgc
    43081 atcgaaccga agcacacgcg tgccgataat cgccgcgcga aggagcttct cggttgggca
    43141 ccgaccattc cgctcgagga aggcgtttcg agcctcaaga aggagttcgg catcgcctag
    43201 cgtatattct ctatgaacgt cgacggaaaa cgactcgcct ccggcgtcta cgaatcgctc
    43261 cgcacgcggg tgcggtctct ctcgcgcgcg ccgcgtctca cgatcgtgac cgcgagcccg
    43321 aacctcgaga cgaagaagta cctcgcgctc aaagagcggc gcgcggccga ggtcggcatc
    43381 gagacgaacg tcatcgttct tgcggagagc gcgacgacgg agaacgtcgt gaccctcgtc
    43441 gcgaacgagg cggctcgtgc cgatgccctc atcgtccaac tcccgctccc gccgtccata
    43501 gatgccgacc gggtgctcgc cgccgtcccc gtctcacgcg atgctgacgg tcttaacccc
    43561 gagagcgccg tcctctcgcc ggtcgtcggt gccatccggg agatactcgc gcgccatggc
    43621 gttttgcccc aggggaagcg tgtcgccgtc gtcggcgagg ggcgcctcgt cggccggccg
    43681 gcgagcgcgt ggtttcggtc cgtcggcgcc caggtgcgcg ttctcacgaa ggacacggcc
    43741 gacatcgaag cggagacgag agacgccgac atcatcgtct ccggtgccgg agtcc
//
LOCUS       DASTSP010000043         9557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_89875, whole genome shotgun sequence.
ACCESSION   DASTSP010000043 DASTSP010000000
VERSION     DASTSP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89875"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFS75_01995"
     CDS             <1..480
                     /locus_tag="VFS75_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4677471.1"
                     /translation="PLYRFLTALSIEHVGEETARIIANAMGSIDRIKTASEDELAAIH
                     GVGDVVARSLVMWFKDAAHRKELDALLREITLEAPEVREGGALRGKSFVFTGTLSSFT
                     REDAEEEVRKRGGSIVSSVSKKTSYVVVGADPGSKAEKARTLGVTVLSEGEFKDLIR"
     gene            548..850
                     /gene="gatC"
                     /locus_tag="VFS75_02000"
     CDS             548..850
                     /gene="gatC"
                     /locus_tag="VFS75_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU4677472.1"
                     /translation="MKREDIEHLAKLSRIALSDAEADSLAGDISKILAYVGDIEKATG
                     AAAPEKKVGPLNTVMREDGEPHESGIYTDALLKAAPERQGPYVKVKKILGDKTDRT"
     gene            847..2268
                     /gene="gatA"
                     /locus_tag="VFS75_02005"
     CDS             847..2268
                     /gene="gatA"
                     /locus_tag="VFS75_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU4677473.1"
                     /translation="MTIRELRKKFVEGTYTPTDAYRDMRAAIDAKNPDLNAFIDVYDD
                     AEAAAKAATQRYATEGENAPALLGMPLAVKNNILIEGKRATAASKMLKNYIAPYDATI
                     IARLKAEGPVIMGATNLDEFAMGSSTENSAFGPTKNPVDPTRVPGGSSGGSAAAVKMG
                     AAVAALGTDTGGSVRLPASFCGLVGYKPTYGKNSRHGLIALGSSLDQAGTFTNSVADA
                     ELLEKVMGGKDRFDATSVGEDTYPAVALKDRYRIGVPRDFLTEGMDADVLAAFEESIA
                     ALVRAGHEVVDVSMPHMKNGLAAYYIVLPAEISSNLARYDGVRFGLHVSGDTLLDDYL
                     KTRAEGFGPEVKRRILLGTYVLSAGYYDAYYGKAELMRSLMREELDHVFSDVDLIMTP
                     TAPTPAFKLGEKSDPLSMYLADIFTVPVNLTGVPAISIPEREVVRDGISLPVGVQYIA
                     PHAGDERLFDFGKKTYDATKGLA"
     gene            2279..2893
                     /locus_tag="VFS75_02010"
     CDS             2279..2893
                     /locus_tag="VFS75_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677474.1"
                     /translation="MNSAPVPDENDIVFRTYGIDYERLARSIYEHLFHGDWSTFTDAV
                     RFWWDLYSIIAIVLSLIFFAGYIYAKIRMAELTAIQNDVLRDAEARWAARHALKGTRN
                     ARWELIQQKIAENNPESWRLAIIEADILLDETLTNAGYVGQTLGDKLKTANPQSFTTI
                     QDAWEAHKVRNEIAHVGSDFVLTKKTAQDTIVRFERVFQEFAII"
     gene            3031..3101
                     /locus_tag="VFS75_02015"
     tRNA            3031..3101
                     /locus_tag="VFS75_02015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3063..3065,aa:Met,seq:cat)
     gene            3222..3899
                     /locus_tag="VFS75_02020"
     CDS             3222..3899
                     /locus_tag="VFS75_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU4677475.1"
                     /translation="MQCVILAAGKGTRLRPLTDNCPKPLVKVGSKTLLDHIVESLPSA
                     VDELIIVVGYLGDMIKAYCGEEFHGRKVKYVEQTVQDGTARALWLCKDLLKGRFLFMF
                     ADDIHGENDIARITSYSRGMLAKTSNTPERFGVVVRNPDGTLFEIIEKPTHAPSNLVS
                     TGVMVLDDHVFEFEPQNPVNGEYYLTEVLERYATKYPVAVVEEHFWIPIGYPEDITRA
                     ETLLSIR"
     gene            3966..4039
                     /locus_tag="VFS75_02025"
     tRNA            3966..4039
                     /locus_tag="VFS75_02025"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4000..4002,aa:Met,seq:cat)
     gene            4125..4198
                     /locus_tag="VFS75_02030"
     tRNA            4125..4198
                     /locus_tag="VFS75_02030"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4159..4161,aa:Asp,seq:gtc)
     gene            complement(4378..4453)
                     /locus_tag="VFS75_02035"
     tRNA            complement(4378..4453)
                     /locus_tag="VFS75_02035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4418..4420),aa:Thr,seq:ggt)
     gene            4700..4772
                     /locus_tag="VFS75_02040"
     tRNA            4700..4772
                     /locus_tag="VFS75_02040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4733..4735,aa:Thr,seq:cgt)
     gene            4961..5494
                     /locus_tag="VFS75_02045"
     CDS             4961..5494
                     /locus_tag="VFS75_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4677476.1"
                     /translation="MDATMEKVRKAGTSVQTKSSETGRIELRVRRLTGEDLLSAASAE
                     RILKVITQLIENEEVLDFTVLSDPDTWVMGIFEGVVVEGEFEDPFLIGMATANFKHSW
                     TGWKIIVDDVIVFDKNRHKGIGRALMEAMEELGALHEPPCKTVVLTSDRNDAFAFYYR
                     IGYTTRGEQQFKKRLKG"
     gene            5570..6445
                     /locus_tag="VFS75_02050"
     CDS             5570..6445
                     /locus_tag="VFS75_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU4677477.1"
                     /translation="MAAETEARIREIEEAMLRPDFWNDSGKAQAMIKELQTLKDEASG
                     KGKYDREPAIVTIVAGAGGDDAEDFARMLVEMYERYVAKRGWDVIVLDANPNDHNGYR
                     NIAFEVAGKDAYGTLRRESGVHRLVRMSPFNASGKRQTSFVLVEVSPRVADLKEANLD
                     EDEVEVTYAKSGGAGGQNVNKRETAVRAVHKPTNISVHVSSERSQQQNREKALALLAG
                     KVYAFKEEQRERELKGLSVEKTVKIEWGSQIRSYVLHPYQLVKDHRTNTEVRDIDRVF
                     AGDIQVFLDAEAREG"
     gene            6464..7147
                     /gene="ftsE"
                     /locus_tag="VFS75_02055"
     CDS             6464..7147
                     /gene="ftsE"
                     /locus_tag="VFS75_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU4677478.1"
                     /translation="MIYFDKVSKVYNGGKSVALHEVTFQVAPNEFVSIVGHSGAGKTT
                     LLKMLIAEDRPSSGQVFFESLDIHSLSRSSLPRFRRKIGTVFQDFKLLPNKTAWENIA
                     FAMEANGRSDEEIGENVPQALELVDLGDKAWNFPHELSGGEKQRVAIARAIVNQPDII
                     IADEPTGNLDPIATYEVVQILKKINDLGTTVIMTTHNKGVIDALGRRVITMDEGRIVR
                     DDAEGKYVL"
     gene            7156..8076
                     /locus_tag="VFS75_02060"
     CDS             7156..8076
                     /locus_tag="VFS75_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU4677479.1"
                     /translation="MITSIKRIVRSGFVGFWRNAFVSLAAIFVLTVTLTVVGGMIFID
                     QLLTASLSQIQSKVDINVYMVPGAEEAPIMDLKSSLESLPDVAEVTYTSSDEALAQFR
                     ARHQNDELTLQALDELGDNPLGASLEIRAKDTSQYESIANFLEAQRGAESPDSPLIDR
                     INFSQNRDAINKLTNIIDTVERTSYITMIVLVVASILIAFNTIRLTIYTSREEISVMR
                     LVGASNMFIRGPFVLQGVMYGVIAGVVTLLILYPVALWLGPGTEAFFSFNIFNYFVNH
                     FGYLFAILVGSGIILGTVSSTLAIARYLRV"
     gene            8079..>9557
                     /locus_tag="VFS75_02065"
     CDS             8079..>9557
                     /locus_tag="VFS75_02065"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU4677480.1"
                     /translation="MPTKQRKTKTRTKQRKYIFVVGGVMSGVGKGVASSSIAMLLQSR
                     GLTVTAIKIDPYINVDAGTMNPTEHGEVFVLENGLETDQDMGNYERFLTKDIPGINYM
                     TTGSVYQAVIGKERNLEYGGRNVQVVPDIPLEVIRRIKKAGDVADADVIITEIGGTIG
                     EYENILFLEAVRMMQSETPDDVAVVMVSYLPVPGSIGEMKTKPTQHAARTLGSAGVRA
                     DFILARGPVPLDMKRKEKIARFCNVRPEHVISAPDVKSVYDVPVNFEKDDLSRRISEV
                     LGIKTKRPADLTKWKNFVARSKNGKDVVKIAVVGKYFSSGDFVLSDVYLSVLEAIKYS
                     AYALHLKPEIHYLGAAQFENKKKLRELDEYDGILIPGGFGSTGIPGKLAVIKYARENK
                     IPYFGLCYGMQLLVIEYARNVARLKDAHTAEVDPASNHLVIDIMADQKEKIEKSDMGG
                     TMRLGEYEAKLKRGSVAQKAYGAETVSERHRHRYEVNPEYVAR"
BASE COUNT         2111 a         2875 c         2816 g         1755 t
ORIGIN      
        1 cccctctatc gattcctcac cgcgctctcg atcgagcatg tcggtgagga gacggcgcgc
       61 atcatcgcga acgcgatggg ctcgatagac cgcattaaaa cggcgagcga ggacgaactt
      121 gccgctatcc atggcgtcgg cgacgtcgtc gctcgctccc tcgtcatgtg gttcaaagac
      181 gccgcgcacc ggaaggagct cgacgcgctc cttcgcgaga taacgctcga agcccccgag
      241 gtgagggagg gcggcgcgct tagaggaaag tcgttcgttt tcacgggaac gctttcgagc
      301 ttcacacgcg aggacgcaga agaggaggtg cgaaaacgag gcggcagcat cgtgagctcc
      361 gtctcgaaga agacgagcta cgtcgtcgtc ggtgccgatc ccggctcgaa agcggagaag
      421 gcgcgcacgc tcggcgtcac ggtcctctcc gaaggcgagt tcaaagacct cattcggtaa
      481 gtctcatcgt cgaaagggcg cgccaacgcg ccctttcgtt ttgtctgaaa tccgctatga
      541 tggggcgatg aaacgagaag atatcgagca cctcgcgaag ctctcccgca tcgcgctctc
      601 cgatgcggag gcagattcgc tcgcgggaga catctcgaag atactcgcgt acgtcggcga
      661 catcgagaag gcgaccggtg ccgcggcgcc cgagaagaaa gtcggccctc tcaacacggt
      721 catgcgcgag gacggcgagc cgcacgagag cggcatctac accgacgcgc ttcttaaggc
      781 cgcgccggag cgtcagggac cgtacgtgaa ggtgaagaag atactcggcg ataagaccga
      841 cagaacatga cgatacgaga gctccgaaag aagttcgtcg agggaacgta cacgccaacc
      901 gatgcctatc gcgatatgcg tgcggccatc gacgcgaaga accccgacct aaacgcgttc
      961 atcgacgtct atgacgacgc cgaagccgcg gctaaggcgg cgacccaacg atacgcgacg
     1021 gagggtgaga acgcccccgc gctcctcggg atgccgcttg ccgtgaagaa caacatcctc
     1081 atcgagggga agcgtgcgac cgcggcatcg aagatgctca agaactacat cgcgccgtac
     1141 gacgcgacca tcatcgcgcg gctcaaagcg gaagggcccg tcatcatggg tgcgacgaac
     1201 ctcgacgagt tcgcgatggg ttcctcgacg gagaactccg ccttcggccc gacgaagaac
     1261 ccggtcgacc cgacgcgcgt tccaggcggc tcctcgggcg gttcggccgc ggcggtcaaa
     1321 atgggtgcgg ccgtcgccgc cctcggcacc gacacgggcg gttccgttcg tcttcccgcg
     1381 agcttctgcg gactcgtcgg ctacaagccg acgtacggga agaactcgcg ccacgggctc
     1441 atcgcgctcg gctcctcgct cgaccaggcg ggcacgttca cgaattccgt cgccgatgcg
     1501 gaacttctcg agaaggtgat gggtggcaag gaccgcttcg acgcgacgtc cgtcggggag
     1561 gatacgtacc ctgcggtagc tctcaaagac cgctatcgaa tcggcgtccc gcgtgatttc
     1621 ctcaccgagg gcatggacgc ggacgtcctt gcggcgttcg aggaatcgat cgccgcgctc
     1681 gttcgcgcgg gccatgaggt cgtcgacgta tcgatgccgc atatgaagaa cggcctcgcc
     1741 gcgtactaca tcgttcttcc cgcggagata tcgtcgaacc tcgcgcgcta cgacggtgtc
     1801 cgcttcggac tccacgtctc gggcgatacg ctcctcgacg attatttgaa gacgcgcgcg
     1861 gaaggattcg ggccagaggt gaagcggcgc attctcctcg gtacgtacgt cctctccgcg
     1921 ggttactacg acgcgtacta cggaaaggcg gaactcatgc gttctctcat gcgcgaggag
     1981 ctcgaccacg tcttctccga cgttgatctc atcatgacgc cgaccgcgcc gactcccgcg
     2041 ttcaaactcg gggagaagag cgacccgtta tccatgtacc tcgcggacat cttcaccgtc
     2101 cccgtgaact taaccggcgt gcccgccatc tctatccccg agcgagaggt cgtacgagac
     2161 ggaatttcgc tccccgttgg agtacaatat atagcaccgc acgcaggcga cgagcgcctc
     2221 ttcgatttcg gcaaaaagac gtacgacgcg acgaagggcc tcgcctaagc ggccacccat
     2281 gaattcggca cccgtccccg atgagaacga tatcgtcttc cgtacgtacg gaatcgacta
     2341 tgagcggctc gctcgctcca tctacgagca ccttttccac ggagactggt cgacctttac
     2401 cgatgccgtc cgcttctggt gggacctcta ctcgatcatc gccatcgtcc tctccctcat
     2461 cttcttcgcg ggatacattt acgcgaagat acgcatggcg gagctcaccg cgatacagaa
     2521 cgacgtgctc cgcgatgcgg aagcgcgctg ggcggcgcgc cacgccctca aaggcactcg
     2581 caacgcgcgc tgggagctca tacagcagaa gatagccgag aacaatccgg aatcgtggcg
     2641 actcgccatc atcgaggccg atatcctcct cgacgagacg ctcacgaacg cgggctacgt
     2701 cgggcagacc ctgggcgaca aactgaagac ggcgaacccg caatccttca caaccattca
     2761 ggacgcgtgg gaggcgcaca aggtccgcaa tgagatcgcg cacgtcggga gcgatttcgt
     2821 tctcacgaag aagaccgcgc aggacaccat cgttcgattc gaacgcgtat tccaggagtt
     2881 cgccatcatt tgatttgcgc tctcacgcga attgcggtaa cgtaagacat tcacggagcg
     2941 agacggtccg ggggccgtcg caagacctcg agcagtcgct gtgagaggta tagaggccca
     3001 gtacgggcga ggaacggtag agacattcat cgcggggtgg aggagaggta cctcgggagg
     3061 ctcataacct ccagaccctg gttcgattcc aggccccgca acaagcacga aaagagccgg
     3121 cttccgccgg ctcttttcgt gctcttctga agtgcctgga atcgaaccgt cacgagactt
     3181 tcattttccc ttcattcccg ttcatgctat aacaaccata tatgcaatgc gtcattctcg
     3241 cggccggaaa gggaacacgg ctccgtcctc tcaccgacaa ctgcccgaaa cccttggtga
     3301 aggtgggttc gaaaacgctc ctcgaccaca tcgtcgagtc gcttccgtcc gcggtcgacg
     3361 agctcatcat cgtcgtcggc tacctcggag acatgatcaa agcgtactgc ggtgaggagt
     3421 tccacggacg caaagtaaag tacgtcgaac agacggtgca ggacggcacc gcgcgggcgc
     3481 tctggctctg taaagacctc ctcaaggggc gcttcctctt catgttcgcg gacgacatcc
     3541 acggagagaa cgacatagcg cgcatcacct catacagtcg cggcatgctc gcgaagacga
     3601 gcaacacccc ggaacgcttc ggcgtcgtcg tccgaaaccc cgacgggacg ctcttcgaga
     3661 tcattgaaaa gccgacccat gctccctcga acctcgtctc gaccggcgtc atggtcctcg
     3721 acgaccacgt cttcgagttc gaaccgcaga atccggtgaa cggcgagtac tatctcaccg
     3781 aggtcctcga acgctacgcg acgaaatacc cggtcgccgt cgtcgaagag cacttttgga
     3841 taccgatcgg ctaccccgaa gatatcaccc gtgcggagac gctcctctcg atacgctaaa
     3901 aataccttga agtaacaagg gtctagtggt attctctttt ggccgttacg cggctctcta
     3961 tttttgtcag tgtagctcag ttggttagag cgagggactc ataagcccta ggtcgctggt
     4021 tcgatcccag ccactgacac acggagtatt gccctttatt ttcgaggcgt gtatactctt
     4081 gccgttcgct tcggccgttt aggaggagcg aaattcgtat aactgcggtc gtagctcaac
     4141 tggttagagc acccgcctgt cacgcgggag gttgccggtt caagcccggt cgaccgcgca
     4201 gataaaagcg ctccccccat gggagtgttt ttatatgcgc gcaagggctt gaacaagcgg
     4261 aacggagtga gctcggggag gaagttctta ggagcgaagc gacttagaaa cttgcccctc
     4321 aagcccggtc gaccgcgcag agaaaaaccg gccgagggcc ggttttttcg tatgttctgg
     4381 agccgttgat cgggattgaa ccgatgacct cttccttacc atggaagtgc tctaccaact
     4441 gagctacaac ggcaaaatgt tttagaactt ggagaagaat agcaaaaaat cagttgaaaa
     4501 caaaccctgg gtatattagg ataagacccg tcgccgttgt agctcagttg gtagagtgag
     4561 caagcgggag tttgtgcaag accttgagca gttgctgcga aaggtgtaca gctcctgtaa
     4621 ggagttgcat tcgtatgaaa aacccataca gtctgcactc tctcggacat gaaagattgc
     4681 cagttttccc gcctcttgag ccgtcttagc tcagttggta gagcattgca ttcgtaacgc
     4741 aaaggtcccg agttcgatcc tcggagacgg ctcaagcggc ggaagaaacg agacgtaaag
     4801 ctcccccatt gggagttttt gcgttcttga ggagacggga atacgccagt atatcgaggc
     4861 tggggataaa tggggcccgt gttcatcgca tcttcatcgc tactcattag agtggtagta
     4921 gagaagggca gttccttacg ggtcatctga aaggaagggt atggacgcca ccatggagaa
     4981 agttcggaag gcgggtacgt cggtgcaaac aaaaagttct gaaaccggtc gaatcgagct
     5041 tcgcgtgcgg cgtctcaccg gcgaggacct cctctcggcc gcgtcggcag agaggatcct
     5101 caaggtgatc acccagctca tcgagaacga ggaggtcctc gacttcacgg tgctctccga
     5161 ccccgacacg tgggtcatgg gcatcttcga gggcgtcgtc gtcgaaggtg agttcgagga
     5221 tccgttcctc atcgggatgg cgaccgcgaa tttcaagcac tcgtggaccg gctggaagat
     5281 catcgtcgat gacgtcatcg tcttcgacaa gaaccggcac aagggaatcg gtcgcgcgct
     5341 catggaggcg atggaggaac tcggcgcgct ccacgagccg ccctgcaaga ccgtcgtcct
     5401 cacgagcgac cgcaatgacg cgttcgcgtt ctactaccgg atcggctata cgacgcgagg
     5461 cgaacagcag ttcaagaagc gtctgaaggg ctgacgaatc gataggcgtt tcccgcgtgc
     5521 tccgcacgcg ggttttttta tggccatcgc gatttgctat actggcccca tggcagcgga
     5581 aacagaggcg agaatacgag agatcgagga ggctatgctt cggccggact tctggaacga
     5641 ctcggggaag gcgcaggcga tgatcaaaga gctccagacc ctcaaagacg aggcgtcggg
     5701 caaggggaag tacgacagag agcccgccat cgtcaccatc gtcgcgggtg ccggaggcga
     5761 cgacgcggag gacttcgcgc gaatgctcgt cgaaatgtac gaacgatacg tcgcgaagcg
     5821 gggatgggac gtcatcgttc tcgacgcgaa cccgaacgac cacaatgggt accggaacat
     5881 cgccttcgag gtcgcgggca aagatgccta cggaacgctc cgccgtgagt ccggcgtgca
     5941 ccgcctcgtt cgcatgtcgc ccttcaacgc gagcggtaaa cggcagacgt cgttcgttct
     6001 cgtcgaggtc agcccgcgcg tcgccgacct caaagaggcg aacctcgacg aggacgaggt
     6061 cgaggtaacc tacgcgaaat ccggtggcgc cgggggccag aacgtcaaca agcgagagac
     6121 ggcggttcgc gccgttcaca agccgacgaa catcagcgtc cacgtctcct cggagcggag
     6181 ccagcagcag aatcgtgaga aggcgctcgc gctcctcgcc ggcaaagtgt acgccttcaa
     6241 ggaagagcag cgcgaacgtg agctcaaggg actctcggtc gagaagacgg tgaagatcga
     6301 gtgggggagc cagatacggt cgtacgttct tcacccgtac cagctcgtga aagaccaccg
     6361 tacgaacacc gaagtgcgcg acatcgaccg cgtattcgct ggagacattc aagtttttct
     6421 agacgcagag gcgcgagaag ggtaaaatga cccaatacaa cgtatgatct atttcgataa
     6481 ggtgtcgaag gtgtacaacg gaggcaaatc ggtcgcgctc cacgaggtca ccttccaggt
     6541 cgccccgaac gagttcgtct cgatcgtcgg acactcggga gcggggaaga cgaccctcct
     6601 caagatgctc atcgccgagg acagaccgtc ctccggtcag gtcttcttcg aatccctcga
     6661 catccactcg ctctcacgaa gttcgctccc gaggttccgt cgcaagatag ggaccgtctt
     6721 ccaggacttc aagctcctcc ccaataagac cgcgtgggag aacatcgcat tcgcgatgga
     6781 ggcgaacgga cgctccgacg aggagatcgg cgagaacgtt ccgcaagcgc tcgagctcgt
     6841 cgacttaggc gacaaggcgt ggaactttcc ccatgagctc tcgggcggcg agaagcagcg
     6901 cgtcgccatc gcccgtgcga tcgtcaatca gcccgatatc atcatcgccg acgagccgac
     6961 gggaaacctc gatccgatcg ccacgtacga ggtggtccaa atactcaaaa agataaatga
     7021 cctcggcacg accgtcatca tgacgacgca taacaagggg gtcatcgatg cattaggccg
     7081 gcgcgtcatc acgatggacg aaggccgcat cgtgagggat gacgcggagg gtaagtacgt
     7141 gctttaatac acaccatgat cacgagcata aaacgaatcg ttcgttccgg tttcgtcggg
     7201 ttttggagga acgctttcgt ctcgctcgcg gcgatattcg tgctcacggt gacgctcacc
     7261 gtcgtgggcg gcatgatatt catcgaccaa ctcctcaccg cgtccctctc gcagatacag
     7321 tcgaaggtcg acatcaacgt atacatggtc ccgggcgccg aagaagctcc catcatggac
     7381 ctcaaatcgt ccctcgagag ccttcccgac gtcgcagagg tcacgtacac ctcgagcgac
     7441 gaggcgctcg cgcagttccg cgcgcggcat cagaacgacg agctcacgct ccaggcactg
     7501 gacgaactcg gcgacaatcc cttaggggcg tcgcttgaga tccgcgcgaa ggacacctcg
     7561 caatacgaga gcatcgcgaa cttcctcgag gcacagcgcg gggcggagtc gccggatagc
     7621 ccgctcatcg accgcatcaa tttcagccag aatcgcgacg ctatcaacaa gctcacgaac
     7681 atcatcgaca cggtcgagcg aacgagctac atcacgatga tcgttctcgt cgtcgcatcg
     7741 atcctcatcg cgttcaatac catccgcctc actatctaca cgtcgcgcga ggagatctcg
     7801 gtcatgcgcc tcgtcggtgc ctcgaacatg ttcatccgcg gaccgttcgt gctccagggt
     7861 gtcatgtacg gcgtcatcgc gggcgtcgtg accctcctca tcctctatcc ggtcgcgctt
     7921 tggctcggcc ccggtaccga ggccttcttc tcgttcaata tcttcaatta cttcgtgaac
     7981 catttcggtt acctctttgc gatcctcgtc ggctcgggta tcatcctcgg gacggtctcg
     8041 agcaccttag cgatcgcccg gtacctccgc gtataacaat gcccacgaag cagagaaaga
     8101 cgaagacacg cacgaagcag agaaagtaca tcttcgtcgt cggcggggtc atgagcgggg
     8161 tcggtaaggg cgtcgcctcc tcctcgatcg ccatgctcct ccaatcgcgc ggcctcacgg
     8221 tcaccgcgat caagatagac ccgtatatca acgtcgacgc gggcacgatg aacccgaccg
     8281 agcacggtga ggtcttcgtt ctcgagaacg gtctcgagac tgaccaggac atggggaact
     8341 atgagcgctt tttgactaag gatattcctg gtatcaacta catgacgaca ggctcggtgt
     8401 accaggcggt catcgggaag gagcgaaacc tcgagtacgg cggccggaac gttcaggtcg
     8461 tccccgacat tccgctcgag gtcatccggc gcatcaagaa ggcgggcgac gtcgcggacg
     8521 cggacgtcat catcacggag ataggaggca cgatcggcga atacgagaac attctcttcc
     8581 tcgaagcggt ccgtatgatg cagagcgaga cgccggatga cgtcgcggtc gtcatggtga
     8641 gctacctccc ggtgcccggt tcgatcgggg agatgaagac gaaaccgacc caacacgccg
     8701 cacgtacctt ggggagcgcg ggcgtccgcg cggacttcat cctcgcgcgc ggcccggtcc
     8761 cgctcgatat gaagcggaag gagaagatcg ctcgcttctg caacgttcgt cccgagcacg
     8821 tcatctccgc accggacgtg aagagcgtct acgacgtgcc cgtcaacttc gagaaggacg
     8881 acctctcgcg gcgcatctct gaggtcctcg gtatcaaaac gaagcggccg gccgacctca
     8941 cgaagtggaa gaacttcgtc gctcgctcga aaaacggcaa ggacgtcgtg aagatagccg
     9001 tcgtcggaaa gtacttcagc tcgggcgact tcgtcctctc cgacgtgtac ctctcggtcc
     9061 tcgaggccat caagtactcg gcgtacgcgc tccatctgaa gcccgagatc cactacctcg
     9121 gcgccgcgca gttcgagaac aaaaagaagc tccgcgagct cgacgagtac gacggtatcc
     9181 tcattcccgg tggtttcggc tcgaccggta tcccggggaa gctcgccgtc atcaagtacg
     9241 cgcgcgagaa caagataccg tacttcggtc tctgctacgg catgcagctc ctcgtcatcg
     9301 agtacgcgcg gaacgtcgcg cgcctcaaag acgcgcacac ggcggaggtc gaccccgcat
     9361 cgaatcacct cgtcatcgac atcatggccg accagaagga gaagatcgag aagagcgaca
     9421 tgggtggtac gatgcgcctc ggcgagtacg aggcgaaatt gaagcgtggc tcggtcgcac
     9481 aaaaggcgta cggcgcggaa acggtctccg agcggcaccg ccatcgctat gaggtcaacc
     9541 ccgagtacgt ggcacgt
//
LOCUS       DASTSP010000044         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_95771, whole genome shotgun sequence.
ACCESSION   DASTSP010000044 DASTSP010000000
VERSION     DASTSP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95771"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..540
                     /locus_tag="VFS75_02070"
     CDS             286..540
                     /locus_tag="VFS75_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677481.1"
                     /translation="MTISKRTALVTLVITALVGSRALFAFFDDPEGPNLLVVLVTAAV
                     IFGVSSVVYKFCSTVSDLRRMLLAISSQVALIAMLYLLGS"
     gene            609..947
                     /locus_tag="VFS75_02075"
     CDS             609..947
                     /locus_tag="VFS75_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEU4677482.1"
                     /translation="MQIENSMLTTAFELPGYRITCNLGMARGIVVRSRSIIGTIGGGL
                     QTIFGGNITLFTSLCERARADALDLMLKHAAEMGANAVVGVRYDATGIGGGITEVLAY
                     GTAVMVEKNR"
     gene            complement(957..1256)
                     /locus_tag="VFS75_02080"
     CDS             complement(957..1256)
                     /locus_tag="VFS75_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677483.1"
                     /translation="MRWNPLISALLAAAYIGGVVLFMHFIEGRRHDTPDTLLDGMGVL
                     SLLVFSAATMGLLFFYQPVVKLIEGKRAEAITYFLKTLGIFGLITVLILTFVSLQ"
     gene            1346..1693
                     /locus_tag="VFS75_02085"
     CDS             1346..1693
                     /locus_tag="VFS75_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677484.1"
                     /translation="MKQYFCIYRVPVATMDEWRANTSPEEMKKQSEELGAKMMAWTEK
                     HKASLVGQGYPLGKTKTVTKDGARDGRNDLNYAQIVEAESLDDAAAIFADSPHMDIPN
                     SFIDVMEIPHPGM"
     gene            1700..2410
                     /locus_tag="VFS75_02090"
     CDS             1700..2410
                     /locus_tag="VFS75_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU4677485.1"
                     /translation="MTSTDVSHRDKALHANSTNWLRAAVLGANDGIVSIAAIVVGVAG
                     ALTDVHSIFISGVAGLLAGAFSMAAGEYISVSSQRDTEQALLAKERRELEEYPEAEFE
                     ELTKIYEEKGLTRGTAETVARELSRGDVFETHAREELNIDPSNLTNPWQASFASFISF
                     TVGGIIPLLPITLLPASVRIEGTFVAATVALVLTGILSAAKSGASHVRVVLRIVLSGL
                     LAMAVTYGVGVVLGATVL"
BASE COUNT          563 a          666 c          715 g          497 t
ORIGIN      
        1 agccgtacgg cccctttgtc agcgatccgg atggagaagt aacgaaaatt gagttaatcg
       61 acccaaagga ctacaagaaa tactttgatt ggggagagat tggcgaccat atcatgcagc
      121 gagctctgga attgaaagcc cagatggatt tagagacgaa ttacgtaaaa tagatctgca
      181 cgagacgcgt cgttttggat cccaaaaact caccaaacgg accaagcagg gcttggccga
      241 ccacggttct aacacgagac ggccagcatg aaacaacaca ctcatatgac catctcgaag
      301 agaactgcct tggtgaccct cgtgattaca gcgcttgtgg gttcccgcgc actgttcgct
      361 ttttttgatg atccagaggg cccgaatttg ctcgtcgttc tcgtgacggc ggccgtcata
      421 ttcggtgtct cgtcggtcgt ctacaagttc tgttctacgg tctcggacct cagacggatg
      481 cttcttgcga tttcgagtca ggtagccttg atagcgatgc tctatctttt aggaagctaa
      541 agacgtcgag tcagagagcg aggaagtgac ggggagaaaa agaaaggcgc ccttgctata
      601 ctagtgccat gcaaatagaa aattcgatgc tcacgacggc gtttgagctg ccaggctatc
      661 gtattacgtg taacctagga atggcacgcg gtatcgttgt gcgttcccgc tctattatcg
      721 gcacgatcgg cggcggactc cagaccatct tcggcggaaa tatcactctt ttcacgtctc
      781 tctgcgaacg tgcgcgagcc gatgcgctcg atctcatgct gaaacatgcc gcagaaatgg
      841 gcgcgaatgc ggttgttggc gtccggtatg acgccacggg aatcggaggc ggcattacag
      901 aagtcctcgc gtacggaacc gcggttatgg tggaaaagaa ccgttaagtt ccaagtctac
      961 tgcaaactca caaacgtgag gattagaacc gtgatgagac cgaagatacc gagcgttttg
     1021 aggaagtacg ttattgcctc ggctcgcttc ccctcgatga gcttcacgac gggctggtag
     1081 aagaaaagta accccatggt cgccgccgag aatactaaga gcgagagaac gcccatcccg
     1141 tcgaggagcg tgtcgggcgt gtcgtggcgg cggccctcaa tgaagtgcat gaagaggacg
     1201 actccgccga tgtaggccgc cgcaaggagc gcgcttatga ggggattcca tcgcatacgt
     1261 ctagtataac gagggaagag ccgtttcggt tccttcatac gagcatcgct cccggcggtg
     1321 tatactattc ccataatctc tcactatgaa acaatacttc tgcatctatc gagtaccggt
     1381 cgcgacgatg gacgagtggc gcgcgaatac atcaccggag gaaatgaaaa agcaatccga
     1441 ggaactcggc gcgaagatga tggcgtggac ggagaaacac aaggcgtcac tcgtcgggca
     1501 gggctatccc ctcgggaaga cgaagaccgt gacgaaggac ggtgcgaggg acggccgaaa
     1561 cgacctcaac tacgcgcaga tcgtcgaggc agagtcgctc gatgacgcgg cggcgatatt
     1621 cgcggatagt ccccatatgg acatcccgaa ctcgttcatc gacgtcatgg agataccgca
     1681 tccggggatg taaaacgcca tgacttcaac cgacgttagc caccgagaca aagccctcca
     1741 cgcgaattcg acgaactggc ttcgcgcggc ggtcctcggc gcgaacgacg gcattgtctc
     1801 gatagcggcg atcgtcgtcg gcgtcgcggg cgcgctcacc gacgtccatt cgatattcat
     1861 aagcggtgtc gcaggtctcc tcgcgggcgc tttctcgatg gcggcggggg agtacatctc
     1921 ggtctcgagc cagcgcgaca cggagcaggc tctcctcgcg aaggaacgac gtgagctcga
     1981 ggagtatccc gaggcggagt tcgaggaact cacgaagata tacgaggaga agggcctcac
     2041 gcgggggacg gcggagacgg tcgctcgcga gctctcccgg ggcgacgtct tcgagacgca
     2101 cgcgagagag gagctcaaca tcgatccctc gaacctcacg aacccctggc aggcgtcgtt
     2161 cgcttccttc atctccttca ccgtaggcgg tatcattccg ctccttccga ttacgctcct
     2221 acctgcgagc gttcgtatcg aggggacgtt cgtcgcggcc acggtcgccc tcgtccttac
     2281 gggcatactg agcgcggcaa agagcggcgc ttcgcatgtt cgcgtcgtcc ttcgcatcgt
     2341 cctctcaggc cttctcgcga tggcggtcac ctatggcgtc ggcgtcgtcc tcggcgcgac
     2401 cgtactataa acatttgaaa cggagagatg tgatactatc a
//
LOCUS       DASTSP010000045         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1435532, whole genome shotgun sequence.
ACCESSION   DASTSP010000045 DASTSP010000000
VERSION     DASTSP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435532"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VFS75_02095"
     CDS             complement(<1..1168)
                     /locus_tag="VFS75_02095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4677486.1"
                     /translation="MTRDTRRERLSFIDATRRSLRRLDRAIQRRTARIFTLNIALWFL
                     FFFVVYLILLFKVNTYETLGDHFFFGLYSVIITGYILSRFLLANFHKQVDVDLSYEPT
                     VTFVVPAKNEESNIAETIERFAEVDYPKEKIEVIAINDGSTDRTYEEMLRVAERVRPI
                     VKRVEVVNWIENRGKRHGMAEGVFRAKHDIVIFIDSDSFIEPDAVTHLVKYFSDPTVG
                     AVSGHTDVYNANQNLLTRMQDLRYYISFKVYKAAESVFGNVTCCPGCCSAYRREYLGE
                     FIDEWLNQRFLGAVCTFGDDRALTNFILRKYNAVYSPEARAYTVVPDNFPQYVKQQQR
                     WKKSWVRETLLASTFMWRRNSLAAFFFYVYVILAFAAPIVFIRAVFFLPLTKSQR"
     gene            complement(1182..2567)
                     /locus_tag="VFS75_02100"
     CDS             complement(1182..2567)
                     /locus_tag="VFS75_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU4677487.1"
                     /translation="MKKIATPVLTERKRAQRRNASAKSIFAHAKNDRARSRNYTVTVV
                     GLGYVGLPLALEALKGGYTVHGVDKNRELVRVLSEGRAPAHLEEANLRFFRRRYQDMR
                     FSETVLRPSDAYVVCVPTPVLPDKTPDLEPLLGATKLIAPHVRRGTLVVIESTVNPGV
                     CEEFVLPIIERETGLRAERDFFFAHCPERINPGDPRWTVATIPRVLGTSGKRSDAAAL
                     SLYESIIKAPITLMSNIKEAEAVKMVENTFRDINIAFVNELAMAFTKAGIDTMNVLRG
                     ASTKPFGFMAHYPGCGVGGHCIPVDPYYLIAYGHRNGFNHRFLEVAREINNHMPEYTV
                     SLLTERLAKRGIDIRGKKVALLGLSYKKDVPDLRESPALDIAEALTKAGAVVATYDPL
                     VLGRSTAKSLDEALDGAVGAIVATNHTVFGELSPEALLRHDIRVIVDGRNCLEKAKFE
                     SAGIDYSGIGR"
     gene            complement(2747..3859)
                     /locus_tag="VFS75_02105"
     CDS             complement(2747..3859)
                     /locus_tag="VFS75_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4677488.1"
                     /translation="MKTTFKQLVELGIGLKHRLARNLNFVTQVKILISLLAYLLVTVA
                     SLVFVITHVKGNIIEGAAASFAAAANGGDVIIADLVADLDNSVTSMLYGTVLVVTFIT
                     VIFGLLITRLALAPLREAFSMQKSFISGIAHEIRTPLSILRMNNELAKFEVEEPSPMA
                     ELLDENLADIDKVNEILNNLLLFDRMGSLDLLRNIEKNDLLDIAKEVATQFRDLAAQK
                     KISIFITGHDVPPVLGNRTAIEQVFFNLIKNAMTYSQSGGRITIECAGVTDDYVIIRV
                     ADTGVGIPQDDLPRIFEPFYRSGKTGKLSGTGIGLAIVLEIMKMHKGSIEAESIEGVG
                     TSFRLAFPRRQVTPLRRLFRNPMSMSKILFDFSRKS"
     gene            complement(3807..4484)
                     /locus_tag="VFS75_02110"
     CDS             complement(3807..4484)
                     /locus_tag="VFS75_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4677489.1"
                     /translation="MRIIVVEDEPRLARALRQGLQKHGFAVDLKSDGKEALDHVLIHH
                     SVYDLVILDLMLPNMEGLQICKAVRDHEINVPILVLTAKNQTQDKVEALNAGADDYMT
                     KPFSLNELVARINARLRRTEHAYPTKLQAQDITLDVGQHKVYRGDKEIVLTVKEFALL
                     EFFMRNVNQVLNREYILDHIWDFDFNSFSNVVDVHVKNLRKKLGKTKTNEDYIQTVSG
                     VGYRFKA"
     gene            4699..>5188
                     /locus_tag="VFS75_02115"
     CDS             4699..>5188
                     /locus_tag="VFS75_02115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4677490.1"
                     /translation="MNIKIKVIVGSTREGRFSEHAAAWIARLAAEKPGTDVELVDLRD
                     YDMPFFDAAVTPSAKTEPYENEAVARFTAKVNEADGFIVVTPEYNHGPSAVLKNAFDW
                     VSPEWNKKAVAFVSYGTVGGARAVEQLRLVAVELQMAPIRQSIQITGERFYPVKTGKA
                     SAN"
BASE COUNT         1036 a         1458 c         1580 g         1114 t
ORIGIN      
        1 gccgctggct tttcgtgaga ggcaggaaga agacggcgcg gatgaacacg atcggcgccg
       61 cgaacgcgag gatgacgtag acgtagaaga agaacgccgc aagcgagttc ctccgccaca
      121 tgaacgtgct cgcgaggagc gtctcccgta cccacgactt cttccagcgc tgttgctgct
      181 tcacatactg cggaaagttg tcgggcacga cggtgtacgc gcgcgcctcg ggcgagtaca
      241 ccgcgttgta cttccggaga atgaagttcg tgagcgcgcg gtcgtcgccg aacgtacaca
      301 cggccccgag gaagcgttga ttgagccact cgtcgatgaa ctcgccgaga tattcgcggc
      361 ggtatgcgct gcaacagccc ggacaacagg tcacgttgcc gaagacggat tccgccgctt
      421 tgtacacttt gaacgagata tagtagcgga ggtcctgcat gcgcgtgagg aggttctggt
      481 tcgcgttgta gacgtccgta tgccccgaga ccgcgcccac ggtggggtcg ctaaagtact
      541 tcacgaggtg cgtcacggcg tccggctcga tgaagctgtc cgaatcgatg aagatgacga
      601 tgtcgtgctt cgcgcggaac acgccctccg ccatgccatg gcgcttcccc cgattctcga
      661 tccagttcac cacctccacg cgcttcacga tcggacgtac acgctcggcg acgcggagca
      721 tctcctcgta cgtgcgatcc gtcgagccgt cgttgatggc gatgacctcg atcttctcct
      781 tcgggtagtc gacctccgcg aaccgctcga tggtctccgc gatgttcgac tcctcgttct
      841 tcgccggcac gacgaacgtc accgtaggtt cgtacgagag gtcgacgtcg acctgcttgt
      901 gaaagttcgc gaggaggaat cgcgagagga tataccccgt gatgatgacg ctgtagagac
      961 cgaagaagaa gtgatcgccg agcgtctcgt acgtgttgac cttgaagaga agaatgaggt
     1021 acacgacgaa gaagaagagg aaccagagcg cgatattgag cgtgaagatg cgggccgttc
     1081 gtctttggat ggcacggtca aggcgccgaa gcgaacgccg ggtcgcgtct atgaaagaga
     1141 gccgttctct acgggtatcg cgtgtcatgg tacgtgtttc gttaacggcc tatgccggaa
     1201 taatcgatgc ctgctgattc gaacttcgcc ttctcgaggc agttgcgtcc atcgacgatg
     1261 acgcggatgt cgtgtcgaag gagcgcttcg ggcgaaagtt caccgaagac ggtgtggttc
     1321 gtggcgacga tcgcgcctac cgcgccgtcg agcgcctcat cgagcgactt cgcggtcgat
     1381 cgtccgagga cgagcgggtc gtacgtcgcc acgacggcac ccgccttcgt gagcgcttcg
     1441 gcgatgtcga gcgcgggact ctcgcggagg tccggaacgt ccttcttgta cgagaggccg
     1501 agaagcgcga ccttcttccc tcggatgtcg atgccgcgct tcgcgagacg ctctgtaagg
     1561 agcgagaccg tgtactcggg catgtggttg tttatctcgc gagcgacctc gaggaaccga
     1621 tgattgaagc cgtttcgatg cccgtacgcg atgaggtagt acggatcgac gggaatgcag
     1681 tgccctccga cgccgcatcc cggatagtgc gccatgaagc cgaacggctt cgtcgacgcg
     1741 ccgcgaagca cgttcatcgt atcgatgccc gccttcgtga acgccatcgc gagctcgttc
     1801 acgaacgcga tattgatgtc acggaacgtg ttttcgacca ttttgaccgc ctcggcctcc
     1861 ttgatattgc tcatgagggt gatgggggcc tttatgatgg actcgtagag ggagagcgcg
     1921 gcggcatcgc tccgcttccc cgatgtgccg agcacccgcg ggatggtagc gaccgtccag
     1981 cgcggatcgc cggggttgat acgttccgga caatgcgcga agaagaagtc gcgctccgcc
     2041 cggagcccag tctcgcgctc gatgatgggg agtacgaatt cctcacagac gccggggttc
     2101 accgtcgact cgatgacgac gagggtccct cgcctcacgt gaggagcgat gagcttcgtc
     2161 gcgccgagga ggggctcgag gtccggcgtc ttatccggaa ggaccggcgt tgggacgcac
     2221 acgacatagg cgtctgacgg gcgcaggacg gtctcggaga aacgcatgtc ctggtagcgc
     2281 cgcctgaaga agcgcaggtt cgcctcctca aggtgcgcgg gcgcgcgacc ttcggagaga
     2341 acgcggacga gctcccggtt cttatcgacg ccatgcaccg tatacccgcc cttcagcgcc
     2401 tcgagcgcga gcggcagacc gacgtatccg agtccgacca cggttaccgt atagttccta
     2461 cttcgtgctc gatcgttctt cgcgtgggcg aatatggact tagcgctcgc gttccttcgc
     2521 tgtgcgcgct tcctctcagt gagtacgggt gtggcgattt ttttcatgtt ttccaaacgt
     2581 ttaggttcta atgtcgtcta ctgtcgtccg tgcttcgtca gtatgcacga gtcgcaatta
     2641 aaaagtgatt aaaataaccg ttaagaaatg atgaatgttt cgattaaaaa gcgatgaacg
     2701 taaaagccgc gcgcccgtgg gcgcgcggct tagcttcatg cgctcttcag gattttcgcg
     2761 agaagtcgaa taatatcttc gacatcgaca tcgggttgcg gaagaggcgg cgaagaggcg
     2821 tgacctgtcg cctcgggaac gcgagacgga aggacgtgcc gacgccctcg atgctctccg
     2881 cctcgatgga gcccttatgc atcttcatga tctcgaggac tatggcgagt ccaataccgg
     2941 tccccgagag cttaccggtt ttccccgaac ggtagaacgg ttcgaagatg cgcggcagat
     3001 cgtcttgcgg tatgccgacg cccgtgtcgg cgacgcggat gatgacgtag tcgtcggtga
     3061 ctcccgcaca ttcgatggtg atgcggccgc cgctttgcga atacgtcatc gcgttcttta
     3121 tgagattgaa gaatacctgc tcgatcgccg ttcgattgcc gaggaccggc ggcacgtcgt
     3181 gcccggttat gaaaatcgat atcttcttct gtgcggcgag gtcgcgaaac tgcgtcgcga
     3241 cctccttcgc gatgtcgagg aggtcgttct tctcgatgtt cctaaggaga tccagggaac
     3301 ccatacgatc gaagaggaga aggttgttga gtatctcgtt caccttgtcg atgtccgcga
     3361 gattctcgtc gaggagctcg gccatcgggc tcggctcctc gacctcgaac ttcgcgagct
     3421 cattgttcat ccgcaggatg ctaagcggcg tacgtatctc gtgcgcgatg ccggagatga
     3481 agcttttctg catgctgaac gcctcccgaa gcggtgcgag cgcgagccgc gtgatgagga
     3541 gcccgaagat gaccgtaatg aacgtgacca cgaggacggt cccatagagc atggacgtca
     3601 cggagttatc gagatcggcg acgaggtccg cgatgatcac atcgccgccg ttcgcggccg
     3661 ccgcgaacga tgccgcggct ccctcgatga tgttcccctt tacgtgcgtg atgacgaata
     3721 cgagactcgc gacggtcacg agaaggtacg cgagaagact gatgagtatc tttacctgcg
     3781 tgacgaagtt aaggttccgc gcgagcctat gctttaaacc gatacccaac tccactaact
     3841 gtttgaatgt agtcttcatt cgtttttgtc ttgccgagtt tcttacggag gttcttcacg
     3901 tgcacgtcga cgacgttact gaacgaattg aagtcgaagt cccaaatgtg gtcgaggatg
     3961 tactctcggt tcagcacctg gttcacgttg cgcatgaaaa actcgaggag cgcgaactcc
     4021 ttgaccgtga gaacgatctc tttgtcacca cgatacacct tgtgttggcc cacatcaagc
     4081 gtgatgtctt gcgcttggag tttcgtcgga tacgcgtgtt cggtgcgtcg caggcgcgcg
     4141 ttgatgcgcg cgacgagctc gttcaatgag aacggcttcg tcatgtaatc gtccgccccg
     4201 gcgttcaatg cctccacctt gtcctgggtc tggttcttcg cggtgaggac gaggatgggc
     4261 acattgatct catggtcgcg aacggccttg cagatctgaa ggccctccat attgggaagc
     4321 atgaggtcga gaatgacgag gtcgtacacc gaatgatgaa tgagaacgtg gtcgagcgcc
     4381 tccttgccgt cgcttttgag gtcgacggcg aacccgtgtt tctggagtcc ctgtcggagg
     4441 gctcgtgcta gcctcggctc gtcttcgaca acgatgattc gcatacaatt cgtaggtgtt
     4501 tccgctgata aacttctgcc cctctttctg gagtcgcccc ggggccctcg aaacatgatt
     4561 gtacacgaat tttatgaact ttacatgaag ggttaatgaa ctgtcgtgac agaagaccga
     4621 acctgagccg tttctttcgc atccacactt catcaatcgt tcattgagcg ctcaccctct
     4681 ctcgtctaca atggtcacat gaatatcaag atcaaggtca tcgtcggaag tacgcgggaa
     4741 ggacgcttta gcgaacacgc cgccgcctgg atagcgcgac tcgcggccga gaaaccgggg
     4801 acggatgtcg agctcgtcga cctccgcgat tacgatatgc cgttttttga cgctgcggtc
     4861 acgccatcgg cgaagacgga gccgtacgag aacgaggcgg tcgcgcgctt caccgcgaag
     4921 gtgaacgagg cggacggttt catcgtcgtc accccggaat acaatcatgg accttccgcc
     4981 gtcctcaaga acgcgttcga ctgggtctct cccgaatgga acaagaaggc ggtcgcgttc
     5041 gtgagctatg gaacggtcgg cggtgcgcgc gccgtcgagc aacttcgact cgtcgccgtc
     5101 gaacttcaga tggcgccgat acggcagtcc atccagatca ccggtgagcg cttttacccc
     5161 gtgaagacgg gcaaggcatc agcgaacg
//
LOCUS       DASTSP010000046         4993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_764730, whole genome shotgun sequence.
ACCESSION   DASTSP010000046 DASTSP010000000
VERSION     DASTSP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764730"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1443)
                     /locus_tag="VFS75_02120"
     CDS             complement(184..1443)
                     /locus_tag="VFS75_02120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4677491.1"
                     /translation="MRKPTTHGMSEIVRDGERLALLRASIPVPAGSAEAEARALAYAE
                     ALLSGAGPYSRHGLLDALGFLGASVRVGAGDGLISISVDTVDTSLQKTLTLLSHIVTR
                     PHFSSSELTRIKKHVANALELSLEDARGRAYEGFVNAFLEPTDRRYVQPLADVMKALA
                     RVTPKDLRRLHDAVRASAWNVVLGGSEESVRRMRRSLSALSIATVSSSHPHTGRTVRG
                     GRTLALVDIPHKQNIEFSIGGPLSLRPTERAYAAFVFGMSVLGIHGGFTGRLMSIVRE
                     KEGLTYSIYANVEKATTSEDGFWRIATFFNPKDAERGIRSTLREIERIRTDGITEDEL
                     RRFKSILATRFALRDDSLVRRVGETLGRMNAGLTEAEHAAQREALLSLSVDEVNDAMR
                     THLSRSFIVIGGAGPVAKVEPLLRKLV"
     gene            complement(1440..2765)
                     /locus_tag="VFS75_02125"
     CDS             complement(1440..2765)
                     /locus_tag="VFS75_02125"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4677492.1"
                     /translation="MSRRNAKATRSKERDTRDIERVRRVGEITEYLLPGNGLRVLYAR
                     RPGTGVVTSNILYFVGSRDEAHGETGVAHMLEHMLFKPTTHDRSAKSEAAAMRFEREF
                     GIVLNANTWKDRTTYYFSYPKAHLERALSIEAERMRDVIMDEAEFAPERTNVLSEYDM
                     YAGDEQFALAVDMTAAAFQSHPYGHETIGFREDIESYTPQMLRRFYERFYVPGNAVLI
                     IVGDLIEREMKEAVLRYFKDVPPGPVPERLEVKEPKQRGRRLVEVRRESKTNVYAIGV
                     KHGPFPTKPWYETMAVFDMLAGGRDSILHKALVDTGLVTRIDTSVEPSRSENLAILFF
                     TLSPKASHDAVRKRMLASLASLNKKTITPYLQKTIAKAVTSEFETRENSLGYVAELVE
                     YVSAGQWTAFFETERMLRSIRPDDVLTRVRDLFVEENTTEGRFIGTKRT"
     gene            complement(2737..3333)
                     /locus_tag="VFS75_02130"
     CDS             complement(2737..3333)
                     /locus_tag="VFS75_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4677493.1"
                     /translation="MAYSYAIEQAIRAASVLHKDHVRKGPVPYPYMTHLFAVAMIVAD
                     YTTDEDTIVASLLHDTLEYTDYTEDELGDDFGGPVKELVLSVTEPLYDKSDRKASAEA
                     RKAYVKQLKDASERALVIVAADRIHTMRSAIEEYLDDHSGFVAEFGTDLEDSLLQYQE
                     ISNTLNRNLKNAILAEFNSVFTEYKNFIRDVQAKRESY"
     gene            complement(3338..3592)
                     /locus_tag="VFS75_02135"
     CDS             complement(3338..3592)
                     /locus_tag="VFS75_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677494.1"
                     /translation="MTMIQNDPELRRIEMQLKRKEDERKEDQRELDRLNAKASELDRD
                     IMKVQAKVNADAQEIARLEASKHDRMTQVQKEAEKEARAK"
     gene            complement(3589..3930)
                     /locus_tag="VFS75_02140"
     CDS             complement(3589..3930)
                     /locus_tag="VFS75_02140"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU4677495.1"
                     /translation="MTSFRESVLSVVRSIPRGRTMTYGEVAARAGRPRAHRAVAGIMS
                     RNVDPTVPCHRVVGADGSMRGYNVFSRPRETLAHDVVEFLERLPYTRGRGGDAESVKR
                     ALLEAEGVIFS"
     gene            complement(3927..4715)
                     /locus_tag="VFS75_02145"
     CDS             complement(3927..4715)
                     /locus_tag="VFS75_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU4677496.1"
                     /translation="MGTFTKKYNPNRGADWNYGGANWRLSRSKIDLFVECPRCFILDN
                     KLGTARPRGPAFTLNVAVDALFKKEFDEYRKDGRPHPLMLQYGLDAVPYSHPMLDTWR
                     ENFKGIEHRHADTGFVVSGAVDDIWVRPDGSLIVVDYKATAKDGTIETLADSAWEEQY
                     KRQIGVYQWLLRQNGFEVSETGYFVYANGDASKPAFENVLSFETTLVPCVGDTSWIEP
                     TLQAVKACLESDALPPSGTTCEFCPYREASGKKLQKIHAAMKRT"
     gene            complement(4778..>4993)
                     /locus_tag="VFS75_02150"
     CDS             complement(4778..>4993)
                     /locus_tag="VFS75_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677497.1"
                     /translation="RDYANDTAGRILGAARYDVKAGLVPTMVELVRPTIAGNTYQVSF
                     YTDEGATTFKSGEDKPIEGGEATFTAK"
BASE COUNT          849 a         1524 c         1553 g         1067 t
ORIGIN      
        1 attccggcgg cggccagggc cgcgccggcg acgtgacctt cagccagggc accatgggcc
       61 tcggcgccgc gagatcctga gcggccgccg gctcggcgcc gtacgagagt agccgtacgg
      121 cggacgtaca ccacgcagga aggggctgac cgagcggtca gccccgtttt tatttacata
      181 cgtctagacg agcttccgaa ggaggggctc gaccttcgcg accggtcccg cgccgccgat
      241 gacgatgaaa ctacgcgaga ggtgcgtccg catcgcgtcg ttcacctcgt cgacgctcag
      301 cgacaggagc gcttcacgct gtgccgcgtg ctcggcctcg gtaagacccg cgttcattcg
      361 cccgagcgtc tctccgacgc ggcggacgag cgagtcgtcg cgaagcgcga agcgcgtcgc
      421 gagtatcgat ttgaagcgtc gaagttcgtc ttcggtgata ccgtccgtac ggatacgttc
      481 gatctcgcga agcgtcgagc gaatcccgcg ttcggcgtcc ttcggattga agaacgtcgc
      541 gatacgccag aagccgtcct cgctcgtggt agccttctcg acgttcgcgt agatcgagta
      601 cgtgagcccc tccttctcgc gaacgatgct catgagtctg ccggtgaatc cgccatggat
      661 accgaggacg gacataccga agacgaacgc cgcatacgcg cgctcggtgg gccgaagcga
      721 gagcggtccg cctatcgaga actcgatgtt ctgtttatgc gggatatcga cgagcgcgag
      781 cgttcgtcct cccctcaccg tgcgtcccgt gtgcggatgg gatgatgaaa cggtcgctat
      841 ggacagcgca ctgagggaac gccgcattct tctcacgctc tcttcgctcc cgccgagaac
      901 gacgttccat gcgctcgccc gtaccgcatc atgtaatcgc cggaggtcct tcggcgtgac
      961 gcgagcgagc gccttcatga cgtcagcgag tggctggacg tatcgacggt cggtcggttc
     1021 gagaaacgcg ttcacgaaac cttcgtaggc tcgcccgcgc gcgtcctcga gcgagagctc
     1081 gagcgcgttc gccacatgct tcttgatgcg ggtgagctcg gagctggaga agtgcggacg
     1141 agtcacgatg tgcgagagaa gcgtgagcgt tttttggaga gacgtatcga cggtgtcgac
     1201 cgaaatggag atgagaccgt cccctgcccc gacgcgaacg gacgcgccga ggaaaccgag
     1261 cgcatcgagc agtccgtgcc gcgaataggg ccctgctccc gagaggagcg cttcggcgta
     1321 cgcgagcgcg cgggcttcgg cctcagcgct accggcaggg acgggtatcg aggctcgcag
     1381 gagcgcgagc cgctctccgt ctcgtactat ctcgctcatc ccgtgtgtcg ttggctttct
     1441 catgttcgtt tggtgcctat gaatcgtcct tccgtcgtgt tctcctcgac gaagaggtct
     1501 cgcactcgcg taaggacgtc gtccggtcgt atcgaacgga gcatccgctc cgtctcgaag
     1561 aacgcggtcc actgtcccgc gctcacgtac tcgacgagct cggcgacgta tccgagactg
     1621 ttctcgcgcg tctcgaactc gctcgtcacc gccttcgcga tggtcttctg caaatacggc
     1681 gtgatcgttt ttttattgag agacgcgaga gatgcgagca tgcgctttcg caccgcgtcg
     1741 tgggacgctt tcggtgagag agtgaagaag agtatggcga ggttctcgct cctcgatggt
     1801 tcgacgctcg tgtcgatccg cgtcacgagt cccgtgtcga cgagcgcctt atggagaatg
     1861 ctgtcgcgtc cgccggcgag catgtcgaag accgccatcg tctcatacca cggcttcgtc
     1921 gggaacggac cgtgctttac cccgatcgcg tacacgttcg tcttcgactc gcgtcgcacc
     1981 tcgacgagtc gtctaccgcg ttgcttcggc tccttcacct cgaggcgttc gggtacgggt
     2041 ccgggtggca catccttgaa ataccgcaag accgcctcct tcatctcccg ttcaataagg
     2101 tcgccgacga tgatgaggac ggcgttcccc gggacgtaga agcgctcgta gaatctacgg
     2161 agcatctgcg gcgtgtacga ttcgatgtcc tcacgaaagc cgatggtctc gtgaccatag
     2221 gggtgcgact gaaacgctgc ggcggtcata tctaccgcga gcgcgaactg ttcgtcgccc
     2281 gcgtacatgt cgtactccga gaggacgttc gtacgctcgg gcgcgaactc cgcctcgtcc
     2341 atgatgacgt cgcgcatacg ctcggcctcg attgagagcg ctcgctcaag gtgcgccttc
     2401 gggtacgaaa agtaataggt cgtccggtct ttccaggtgt tcgcgttaag gacgatgccg
     2461 aattcccgct cgaaacgcat cgccgcggcc tcgctcttcg cgcttcggtc atgggtcgtc
     2521 ggcttaaaga gcatgtgctc gagcatgtgc gcgacgcccg tctcgccgtg cgcttcgtcg
     2581 cgcgaaccga cgaagtagag aatattagat gtcacgacgc cggtgccggg tcgtcgcgca
     2641 tagaggacgc ggagcccgtt acccggaagg aggtactccg ttatctcgcc gactcgccgt
     2701 acgcgttcta tgtctcgtgt gtctcgttct ttcgatctag tagctttcgc gtttcgcctg
     2761 gacatcgcgg atgaagttct tatattccgt aaagacgctg ttgaactccg caagtatagc
     2821 atttttgaga ttcctgttga gcgtgttcga tatttcctgg tactggagca gtgaatcctc
     2881 gagatcggta ccgaactccg cgacgaatcc cgagtggtcg tcgaggtact cctcgatagc
     2941 cgagcgcatg gtgtggatgc ggtcggcggc gacgatgacg agcgcgcgct cggacgcgtc
     3001 cttcaattgc ttcacgtacg ctttgcgcgc ctccgccgag gcctttctgt cgctcttgtc
     3061 gtagagaggc tcggtgacgg agaggacgag ctccttcacg ggtccgccga agtcgtcccc
     3121 gagctcgtcc tcggtgtaat cggtatactc gagcgtatcg tgaaggagcg atgcgacgat
     3181 ggtgtcctcg tcggtcgtgt aatcggcaac tatcatggcg accgcgaaga ggtgcgtcat
     3241 gtacggatac ggcacgggac ctttccgcac gtggtccttg tggaggaccg acgccgcgcg
     3301 tatcgcctgt tcgatagcgt acgagtaggc catgtcgtta tttggcacgc gcctccttct
     3361 ccgcttcctt ctgcacctgt gtcatacggt cgtgtttcga cgcttcgagc ctcgcgatct
     3421 cctgtgcgtc ggcgttcacc ttcgcctgga ccttcatgat gtcgcggtcg agttccgagg
     3481 ccttggcgtt cagtcggtcg agctcacgct ggtcctcttt gcgttcgtcc tcttttcgtt
     3541 tgagttgcat ctcgatacgg cgcagttccg ggtcgttctg gatcatggtc atgaaaagat
     3601 tactccttcg gcttctaata gggctcgctt caccgattcg gcgtcgccac ctcttcctcg
     3661 agtgtacggc aagcgctcca aaaattcgac gacatcatgt gcaagggtct cgcgcgggcg
     3721 cgaaaagacg ttgtagccgc gcatcgagcc gtcagcgccg acgacgcgat ggcaaggcac
     3781 ggtcgggtcg acgtttcggc tcatgatgcc cgcgaccgct cggtgcgcac gcggacggcc
     3841 tgcgcgcgcc gcgacctcgc cgtacgtcat cgtccgccct ctcggtatcg agcgtacgac
     3901 ggagagcacc gattcgcgga atgaggtcat gtccgtttca tcgccgcgtg gatcttctgg
     3961 agctttttac cgctcgcctc gcggtacggg cagaactcgc acgtggtgcc cgacggcggg
     4021 agcgcatcgc tctcgagaca cgccttcacg gcctggagcg tcggctcgat ccacgacgtg
     4081 tcgccgacgc acgggacgag cgtcgtctcg aacgaaagga cgttctcgaa cgccggtttg
     4141 ctcgcatcgc cgttcgcgta cacgaagtat ccggtctccg agacctcgaa gccgttttga
     4201 cgaaggagcc actggtacac gccgatctgg cgcttgtact gctcctccca tgcggaatcc
     4261 gcgagcgttt cgatggtgcc gtccttcgcg gtcgctttgt agtcgacgac gatgagcgac
     4321 ccgtccggac gcacccagat atcgtcgacc gcacccgaga ccacgaagcc ggtgtcggca
     4381 tggcgatgct cgatgccttt gaagttctcg cgccacgtgt cgagcatcgg atggctgtac
     4441 gggacggcgt cgaggccgta ctgaagcatc aaggggtgcg gtctcccgtc cttccgatac
     4501 tcgtcgaact ccttcttgaa gagcgcgtcg acggcgacgt tgagcgtgaa tgccggaccg
     4561 cgcggccgcg ccgtcccgag cttgttatcg aggatgaagc accgtggaca ttcgacgaag
     4621 aggtctatct tcgagcgcga gagccgccaa ttcgcgccgc cgtagttcca gtcggcaccg
     4681 cggttcggat tgtacttctt cgtgaaggtt cccatgagaa aagtataaca tccgtgagac
     4741 acgaaaaagc acccgtgtgg gtgcttgttt cgaggctcta tttggccgtg aaggtcgctt
     4801 cgccgccctc gataggcttg tcctccccgg acttgaaggt cgtcgctccc tcatcggtgt
     4861 agaaggagac ctggtacgtg ttacccgcga tggtcgggcg aacgagctcg accatggtcg
     4921 gtacgaggcc ggccttcacg tcgtagcgcg cggcgccgag aatgcgtccg gcggtgtcgt
     4981 tcgcgtagtc gcg
//
LOCUS       DASTSP010000047        23615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_990629, whole genome shotgun sequence.
ACCESSION   DASTSP010000047 DASTSP010000000
VERSION     DASTSP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 23615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23615
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990629"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFS75_02155"
     CDS             <1..398
                     /locus_tag="VFS75_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4677498.1"
                     /translation="GDHAGGALAGFINGALAKTGGEHPDVQAWYASFLHDAVLPNVYL
                     WSHMVVLGEMLVGIALIAGFLVGISAFIGTFMSFNFLLAGTVSINPFLVAFGILLVLA
                     WRVSGYIGLDYYVLPMFHRYLRPKRVLWS"
     gene            complement(439..852)
                     /locus_tag="VFS75_02160"
     CDS             complement(439..852)
                     /locus_tag="VFS75_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677499.1"
                     /translation="MNAKTLRELAKFASGLVAADFLIGLFVLFGGNLPMTFFGMNFDE
                     RVTIEWMLFDVVLFAFLVHYGWRTGDVPRSDRERTFHRVAGILLAVVAVLHLLRILFG
                     VDLTIGSWEAPYWLSAIGAVVTAFLAYMSFTLARR"
     gene            981..1706
                     /locus_tag="VFS75_02165"
     CDS             981..1706
                     /locus_tag="VFS75_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU4677500.1"
                     /translation="MLLSLIALAAFASTLIGGLFALRLRDRLHLVLGFSAGAVIGVAF
                     FDLLPESLALTSGAFDTGTVTAVVALGFLAYMLLDRLVLFHGQAEAHEHGTHAHRGIV
                     GAGSLSFHSFLDGMAIGLSFQVAPAVGIIVTTAVLVHDFSDGINAVNLIMRHGGERTQ
                     ALRWLITDAVAPVLGIAAASFFALSEVALGMMLALFTGFFLYIGAGDLLPESHHAHPK
                     ALTTVMTALGIGVMYVAVSLAGV"
     gene            complement(1949..2020)
                     /locus_tag="VFS75_02170"
     tRNA            complement(1949..2020)
                     /locus_tag="VFS75_02170"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1986..1988),aa:Asn,seq:gtt)
     gene            2362..3018
                     /locus_tag="VFS75_02175"
     CDS             2362..3018
                     /locus_tag="VFS75_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU4677501.1"
                     /translation="MPDGGREKESIMRSFTTRSARRAYALLSLMTAIVFIGGFVAVSR
                     KAEAPVADLSNNETRMALTLTSPAFEDGGVIPSEYTCDGANVNPELRIGGVPEGTRSL
                     VLVMDDPDIPESVKQARGIDVFDHWVLYDIPPTTDSIPKGAPVGTAGLNGLGVAGYAG
                     PCPPDREHRYIFRLYAVSGTLEFVKAPSLKEVEDAAKGMAIESATLIGRYNRPVNVTK
                     "
     gene            3032..3307
                     /locus_tag="VFS75_02180"
     CDS             3032..3307
                     /locus_tag="VFS75_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU4677502.1"
                     /translation="MTTLYVKTGCPFSAKAIGALDAYGVPYELKNIADPGVADELTEK
                     GGKLQVPFLEDGETSMYESDGIVAYVESTYGTGSAEIETTDGGTCPA"
     gene            3313..3549
                     /locus_tag="VFS75_02185"
     CDS             3313..3549
                     /locus_tag="VFS75_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU4677503.1"
                     /translation="MYVRVHALPGAKRERVTRDDEFTFTIAVREPAERNLANRRIAAV
                     LARELSVRENDVRLITGHRSSAKLYSIGERRLQD"
     gene            3562..3930
                     /locus_tag="VFS75_02190"
     CDS             3562..3930
                     /locus_tag="VFS75_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEU4677504.1"
                     /translation="MTFPRISMKTTNITVSPDLEALIEHKFEALGRLLPDGKEALCEI
                     ELEKETEHQSGKIYRAEVNLTVAGKLFRTEATEEQIEHAIDVARGELKRELERAHGKR
                     QSLLKRGGQAIKRMIRFGRD"
     gene            complement(3927..4784)
                     /locus_tag="VFS75_02195"
     CDS             complement(3927..4784)
                     /locus_tag="VFS75_02195"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU4677505.1"
                     /translation="MDIPRIAFFGTPDIGVYVLDELEAAGIVPTIVITNPDAPRGRKL
                     VLTETPVAAWARERGIPCLKPESLDADDVLASLRKSGVELFIIAAYGKRIPSAILALP
                     RYGALNVHPSLLPALRGASPIRSAILENVNPTGVSIMVPVERMDAGPVVAREEVRIPE
                     SEWPTRGRTLDELLARRGGALLARILPDWISGTLTAEAQDESRATYAKKITKEMGEID
                     VAADPSENLRKIRAFDGWPGAYFFTEKGGRRIRVKIVDARLTDGSLVITRVIPEGKQE
                     MDYETFLRS"
     gene            complement(4786..5328)
                     /gene="def"
                     /locus_tag="VFS75_02200"
     CDS             complement(4786..5328)
                     /gene="def"
                     /locus_tag="VFS75_02200"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4677506.1"
                     /translation="MATLVNQNHPVLHEIAAEVPRDEISSPKIKKVLKDMRTALETYN
                     VKGFNGVAIAAPQIGVPLRIFLVHNTSTDPEDRDTLPTLVAINPVLVKLSKRKHIVGE
                     GCLSVGKDYGAVKRSTHATLRALDENGEEYERGASGLLAQIFQHEVDHLDGILFIDKA
                     EKVWNKEDIEAKKLSEASND"
     gene            complement(5386..7269)
                     /locus_tag="VFS75_02205"
     CDS             complement(5386..7269)
                     /locus_tag="VFS75_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677507.1"
                     /translation="MYVITVVPLKRGIALDALSYFGTDRYEPGTIVTIPFRNGSILGL
                     VTETAPVSAAKTALRAATFSLRRLPAQTEATVLGNAFIETAKELSRTYVAPLGTMLYH
                     LLPPEIRSGDVKLPHTHHPGPATGGTVSVLQASRRERYLAFRSLVRETFAHAGSVLLV
                     APSSQDAYELRDALGQGIGDRVVLLSNAQAKTEQKRSFEALEDFSKTKLIIATPSYAM
                     IERHDVTLVIIEHERSPYYKELSRPYVDYRDALLAHARHTGRNVILADLLVRTEDEEA
                     RRRDIYHTYGETPKRIELPGKLEVVEMARAEGEAFRLFSPAVIEAIKDVRKHRGRTFL
                     FAARRGLAPVVACVDCGYIFRSPESGAPYSLLRTTKDGVEKRWFVCGSSGERIRAEDT
                     CRECGSWRLKERGIGIQQVYDELHKLFPNAPTILFDHMSARTFKKASFLRDTFYKTKG
                     AILLGTPMAVPYLTKDVDLSVVMNMDALLSTPTWKLDEENLALLLSLREMTTGTVYVQ
                     TRAPKTDVLLEAKQGSVEHFYTEELALRRSFEYPPFTTFVHLTWQGTPDDVKRTEQDI
                     AARFADFSPSIYQSPVSPLLEPIMYALIKLPRGTWPNDKLSNLLRSLPPTVRVVLNPD
                     KIV"
     gene            complement(7307..8809)
                     /locus_tag="VFS75_02210"
     CDS             complement(7307..8809)
                     /locus_tag="VFS75_02210"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4677508.1"
                     /translation="MKSNIGLHIADLRETQGITQEELARRLKTTQSAVARLESGKQNV
                     SAEMLTKLGRALRKNLVTLSPGTVNLEIEGGRKLKGTITTATSKNGAVGLLCASLLNR
                     GTTTLRNVPRIEEVHRLIEVLQSIGVRVEWDKHDVIITPPRTLDLKAIDTAAARKTRS
                     IVMFIGPLIHAFRSFSLPQSGGCKLGSRTVRPHFFGLEHFGVKIDTTEDEFRVSVKAR
                     RPEEIVLYESGDTVTENVLMAAALTPSTTVIKYASANYQVQELCAFLRVCGVTIEGVG
                     TTTLTVTGVDKLRRDIEYTLSEDPTDTMFFLAAAIVTRSEITIKRCPIEFLELELLKL
                     EKMGFTYVKSKPYLAKNGLTKLVDITTKPSRLMALHEKIHPSVYPGLNIDNLPFFAVI
                     ATQAEGQTLIHDWVYEKRALYFTELDKLGAETVLADPHRIYITGPTTLRPNELVCPPA
                     LRPATILLIGMLGAKGRSVLRNIYSINRGYENLVERLTSLGAEVSYLEEY"
     gene            8906..9916
                     /locus_tag="VFS75_02215"
     CDS             8906..9916
                     /locus_tag="VFS75_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU4677509.1"
                     /translation="MKGFTPKIGIDLGTTNVLVFVHGKGIVLNEPSVVAVSDQNEIVA
                     VGNEAKLMLGRTPDTIRAYRPMKDGVIADYRVTEAMLRYFLKKALGTFNIWRPDVLVS
                     VPAGVSSTEKRAVIEATLKAGARNVYVVKEPILAAIGAKVPIYEPRGHMIVDIGGGTI
                     DVAVISLGGIVASNSVKVAGNKIDAALIDHAKKTLNLSIGEKTAEDIKIKIGSALPLE
                     EELVVEIKGRDVLTGLPRTAEMKTNEVVRAISRELRDMVKAIKDVFQITPPELASDII
                     DHGIIMTGGTSQLRNFPELIKRRTGVKAVLADQAYFCVAKGTGEALEHLDTYKRTIMA
                     KR"
     gene            9976..12702
                     /locus_tag="VFS75_02220"
     CDS             9976..12702
                     /locus_tag="VFS75_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU4677510.1"
                     /translation="METPGREKFSSPQEEIAFLRNEIARRERDLLSRSNEVDHNDVEA
                     VGREVIREYGEHEPDLLLHKSHAIDAYDLAASSETVKTAAEKVEEIMRVAEARGIRNA
                     LAVLERVKDPFLTDEVHRHLVSRLRTERKVADLETDAPLWKVLDMTLYEVALPRDPND
                     EHEHSLKAIFAGMEQFYAGMQTISQGKDPRYYTLEIAVSDKRDDIIFYVAVPNEFANL
                     FEKQALSLFPAAELVLQQNDYNIFVDGGESMIAIAEEKKHHIYPLKTHDEFEVDPLTV
                     LLNAFSKIERDGGGASVQIVVTGGAEKYHRTYGGIVRRMRNGEPTDVAIRKSTLGGEL
                     FEGVKSLIMNSPKKKDDERPHELDTERIELFNRKMSAPVLGINIRLAVSAKDKTRAEQ
                     ILSELESSFHQFQDTQGNQLVFKRLSGVEKAGELRAFSFRELESSRIIPLSITELSTI
                     IHFPAEGVTSSPQFKQSRAKYAAAPVDMPEVGTLLGENEFRGAKRPVYLTPIDRLRHF
                     YVIGQTGTGKTTLLKNMLIQDIEDGAGVCMIDPHGTDIVDVLSAIPPERMDDLVYFDP
                     SNLDRSLGLNMLEYDERHPEEKTFVVNELFSIFQKLYGANPESMGPMFEQYFRNATLL
                     VLEDPASGSTLLDIARVMTDGKYRARKLARAKNPVVIQFWTAIATKAGGEAALENIVP
                     YIVSKFDVFTANDYMRPIIGQQRSAFDFRKLMDERKILLVNLAKGRLGEINANLIGMI
                     IVGKLLMAALSRVDDLTMSYPPYYLYIDEFQNVTTNSIASILSEARKYKLGLTIAHQF
                     IAQLDEPIRDAVFGNVGSLAAFRVGPEDAEFLEKQFSPTFTAQDLMNVENRNAYVRIL
                     SNGMPTPPFSIRTLPPRETNHEYAAEMIRYSHAQNGRPREEVEREIRERFLS"
     gene            12796..14484
                     /locus_tag="VFS75_02225"
     CDS             12796..14484
                     /locus_tag="VFS75_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677511.1"
                     /translation="MRDEDAGTAPSFDLRSWQGLTEVLKRGKESLADPHAYAEFRNLV
                     LSYAQQGGNAELRKQIDSIVASFTTIARDEASLSEESAPAPARKIVDPPVTKTEEAPP
                     SEVPPKRVGIGMRRLAPRFSASVSKPKPVVLPTKTESTPPPRPAAPKPEPTPVPPDVT
                     TPKPTTPSSPKPMVAEKSPAEQASEPVYETKPASGAPPAPMSEPAPTSASSVKEASTG
                     ASSSFKSLDEHKARIAEIKRLVNAQIGNPATLIDTHNEIGKRYMKSLLNALKATGGGG
                     GNVDDSMAELESAFDALRNDAPEAKSGTVPAIPPAPEPTPEPVAEEVPRSDTPMPAVK
                     EEAPEWDARADAIQKNDEKILSVMRDMQKDRSGGEVADLASAIANGRTEAAVKEAVVA
                     EVPKAPREPEPMKVASAMAEAPRNRVDFVPQNKNVVREVGKRPIEDVKKETGVDPREV
                     AIKQTELSSPEITEALHQLLNEWSIFRGRGFFGMGPSGPEHPLYHTLAPLSMGEVIAG
                     RWEGSDRKLTKMIKQYVDAWRHEQGIAYVITETFEHYLRRVVQRIMKRGQGGGA"
     gene            14529..15125
                     /locus_tag="VFS75_02230"
     CDS             14529..15125
                     /locus_tag="VFS75_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677512.1"
                     /translation="MHVHHAQLLVGSLEWASAFVPESERTPGTDVTVIDTDLLSIATV
                     RELSRAAALRPVSRPYRTFVVSARGVPHEAQNALLKLLEEPNENTLFYVIVPREDVLL
                     ATVRSRLHLVATEDSATSSHFEAFLGLSHAKRLAMIADRVTAEDAPWVADILRGMEAY
                     ARKRRDPSLIEEILKVESYLAGTGASKKMLLEHLALSL"
     gene            15158..15709
                     /gene="frr"
                     /locus_tag="VFS75_02235"
     CDS             15158..15709
                     /gene="frr"
                     /locus_tag="VFS75_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU4677513.1"
                     /translation="MAYNPQQFVARGDEIVKWLESEFSSVRTGRATPALLDLVLVESY
                     GARVPIVQVGSVSVEDPRTLRVTVWDKTAIKAVEKAIVDADLGVSVVADGSGLRVIFP
                     ELTGERRAQLIKIAKAKLEEARVSIRGARDEAVKELEALQKSGELSEDEKFSAKEDLQ
                     KRVDAYNAKLVAAFELKEEEINA"
     gene            15714..16832
                     /locus_tag="VFS75_02240"
     CDS             15714..16832
                     /locus_tag="VFS75_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4677514.1"
                     /translation="MSILIFLGVLFALVLVHELGHFAVAKWTGMRVDEFGIGFPPRLF
                     GVRKGETEYTLNLFPIGGFVRIFGENGDEAGGEVNDDGASSVPSDGGRSFTDKSRWAQ
                     AAVLVAGITMNILFAWLLISAAFAMGVETSVDESEAGPGARLVVLNVLPESPAASALD
                     AGDVIESASAGGESIESPTPSAFSEFIGRHGDEAVTLAYRRGSERGIAEVTPKAGVVS
                     AEASRPAIGVSLSMVEKERRAVGAAIVDGFTYTFTSLKETALGIGGLLYDAIRGRADL
                     SQVAGPVGIVGLVGQASAYGLSALLMFTAFISLNLAVINILPFPALDGGRLLFVAIES
                     VKGSPIPARYAGIVNAIGFVLLIALMVVITVHDVSKLI"
     gene            complement(16884..17228)
                     /locus_tag="VFS75_02245"
     CDS             complement(16884..17228)
                     /locus_tag="VFS75_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677515.1"
                     /translation="MSEIRTINAVLETHPQKAAFVENGTSKKRRDFTMREARFDRSFG
                     NFGIFVLVVEDETGARLHIGTEPGERFWRYLHGDPMRGEMLCYSIRTPTLTLLDGLDA
                     QMLDELMRTLAS"
     gene            17419..18684
                     /locus_tag="VFS75_02250"
     CDS             17419..18684
                     /locus_tag="VFS75_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEU4677516.1"
                     /translation="MKQSRLFTKTRKEVPADEVARNAQLLIRAGYVAKEMAGVYAYLP
                     LGLRVLERIKSIVREEMDAIGGQELIMTALQRKEVWERTNRWSDEAVDVWFKSELKAG
                     GEVGFGWSHEEPIGEMMKSYIASYKDLPVYVYQFQTKLRNELRAKSGIMRGREFVMKD
                     MYSFSVGEEDHTAFYDATIEAYKRVFERVGIGADTFVTFASGGAFTQFSHEFQTITDA
                     GEDVIYIQREKGIAINAEVLNDDTLRELGVTREELTEVKTAEVGNIFNFGRQKAEDIG
                     LVYKNEAGESVPVWMGSYGIGVTRLMGVIVEKFADEKGMVWPEAVAPFPVHLIELSGQ
                     DAEIKEYADELYSGFKDHGVEVLYDDRDARAGEKFNDSDLMGIPYRVVVSRKTKEEGK
                     YEVVNRRTGEVEHLDEESLYEKFVNARAA"
     gene            18681..19751
                     /locus_tag="VFS75_02255"
     CDS             18681..19751
                     /locus_tag="VFS75_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU4677517.1"
                     /translation="MSKYPGYIDRLMGTLSTDIGIDLGTANTLVYLKGKGIVLNEPTI
                     VAINRKTGQLVAVGNDAKTMLGRTPAHIEVVRPLVHGVISDFEVTEEMIAYLINKVRS
                     GSRSLIAPRVLIGVPSGITSVEMRAARDAAKNAGARWVYLIEEPIAAAIGAKLPIHKP
                     QGSMIIDIGGGTTDIAVMSLNGIVTARNLRVAGDQLNEDIMAYIRDQFKVLVGDRTAE
                     DTKIALASLEESGDSKELIVRGRDVMTGLPREVIITDSDIREAIMGSLNQIVEATRDV
                     IELTPPEVLADVMQRGITLCGGGALIPGVPKLLENVLKLPVIVVPDPLRAVMRGIGSV
                     LEGLDIYEELLIENEGELSPQI"
     gene            19702..20622
                     /locus_tag="VFS75_02260"
     CDS             19702..20622
                     /locus_tag="VFS75_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU4677518.1"
                     /translation="MKSYLSKTRESSLRRSDAGGLRRLVAWIVVAFLVLLLARGPLGA
                     LGSMVTSPAFSFRHWLETSSATIPVFVRSRLELESEINDLRSKLAAEEGRNAAFSFVT
                     DENRELRALFASSSSPRILAGVISRPPLTPYDTLIIDRGAEDGVIEDAPVYFGNDRAV
                     GYVRTVFSGSALVSLFSSPGAESTVYVFGPNIFTTAEGEGGGVIHLSVPQGIEVKAGD
                     VVVLPSLDGGVLGLVDHVTAEPTEPEQDAYVLYDVPLQSVRLVAVGTTPVRPVSWSEA
                     TDEVDRARAVFTFDIPSTTPEKIAPSTTTP"
     gene            20628..20870
                     /locus_tag="VFS75_02265"
     CDS             20628..20870
                     /locus_tag="VFS75_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677519.1"
                     /translation="MRIIAFICLFFAALYLPLWLFALGAVAYAFTFRAYELLVLAVLI
                     DAEFGEPGTGAYRYTASVAIALLFAALVRPYLRFYA"
     gene            20867..22552
                     /locus_tag="VFS75_02270"
     CDS             20867..22552
                     /locus_tag="VFS75_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU4677520.1"
                     /translation="MRFFRKKRRAEISFDEILLDSSNLPSFNTGRLEGRIELPLQKRN
                     VYVVGGIFAVIALVFFSQLVNLQVIQGAELNAKSESNRLDQSIIIAERGIIYDRYGEL
                     LAWNERDAAGKYDFPVRAYTDRRGFGQLLGYVSYPQRDKAGFYYRTEYIGRNGVESAY
                     DDVLRGENGRHLIEENVHGDTISENAVDEPVPGKPLSLALDAELSEVMYDLIASTTAE
                     NGFRSGAAAMMDVDTGEIVALASFPSYDPEVLADGDDVAKIEEYNADKRFPFLDKVMG
                     GAYVPGSIVKPIVAYGALAEHIISPDKVIVSTGSLTIPNPYDPSHPAVYRDWNPSLGA
                     MTIREGLAFSSNVFLMTIGGGFGDQKGLGIAKLDKYYRLFGLGQRTGVPLDGEQAGVV
                     PDPVWKEKVFKDEWRLGDTYLTSIGQFGFQVTPLQMLRAYAAIANGGKLVTPQVVLGA
                     EGDTVDLHLDQSALTVVKEGMREAVTKVGGTARALDRKDVMVAAKSGTAELGTSKAYV
                     NSWVAGFFPYEHPKYVFILLMEYGPRTNTVGSSSVMGKVFEWMATHRKEYFGA"
     gene            22613..23086
                     /gene="greA"
                     /locus_tag="VFS75_02275"
     CDS             22613..23086
                     /gene="greA"
                     /locus_tag="VFS75_02275"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU4677521.1"
                     /translation="MNEPHDSFLTQEKFEELKKELYHLKTVRRKEVAESLEYARSLGD
                     ISENAEYQEARDMQAAIEERIQYLEKVIKEAKIVATTKKGDVVALGSEVTIQKEKTNE
                     AHTYTIVGSEEANIHERKLSFLSPLGEALMGKAKGDTFTFETPNGKQSYTVTKVA"
     gene            23313..>23615
                     /locus_tag="VFS75_02280"
     CDS             23313..>23615
                     /locus_tag="VFS75_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU4677522.1"
                     /translation="MSALEEIRNERLKKLELLKEKGIDPYRATTTRTHTIAEADGRFD
                     ELAASGEQVTLAGRVMVVRGQGAILFADLQDGTGKFQAVVKEDTLAADSFALFRDAV"
BASE COUNT         4613 a         7136 c         7436 g         4430 t
ORIGIN      
        1 ggggagacca cgcgggcggg gcgctcgcgg gattcattaa cggcgctctc gcgaagacgg
       61 gaggcgagca tccggacgtc caagcctggt acgcctcgtt tctccatgat gcggtccttc
      121 cgaacgtgta tctctggtcc cacatggtcg tcctcggcga aatgctcgtc ggcatcgcgc
      181 tcatcgcggg cttcctcgtc ggtataagcg cgttcatcgg aacgttcatg agcttcaact
      241 tcctcctcgc cggcacggtg agcatcaatc cgttcctcgt cgcgttcgga attcttctcg
      301 tccttgcgtg gcgtgtgagc ggctacatag gactggacta ctacgttctt ccgatgttcc
      361 atcggtacct tcgcccgaag cgcgttctct ggtcatagac atgaaaaaac acgcaccggg
      421 gtgcgtgttt tttcatgtct agcgtcgcgc gagcgtgaag ctcatgtacg cgaggaacgc
      481 ggtcacgacc gctccgatcg cgcttagcca gtacggcgct tcccacgaac cgatcgtgag
      541 gtcgaccccg aagaggatgc ggagaaggtg gaggaccgcg acgacggcga gtaggatacc
      601 tgcgacgcgg tggaacgttc tctctcgatc gctccgcggg acgtccccgg tacgccatcc
      661 gtaatggacg aggaaggcga agaggacgac gtcgaagagc atccactcga tcgtcacgcg
      721 ttcatcgaag ttcattccga agaacgtcat ggggaggttc ccgccgaata ggacgaagag
      781 gccgatgagg aagtctgccg cgacgagtcc ggacgcgaac ttcgcgagtt cccgaagtgt
      841 ttttgcgttc atgtggtaag ggttactggt ataggttcat tatagagagt gagcgcggga
      901 tggtctcctc ggttatgcac cgtgcgcgtt tccccggccg tattgcggcc tcgcgccgtg
      961 ggcgctatca tggatggcac atgcttctct ccctcatcgc tctcgccgcg ttcgcctcga
     1021 cgctcatcgg cggactcttc gcccttcgtc tccgtgaccg actccacctc gtactcggtt
     1081 tcagcgccgg cgcggtcatc ggcgtcgcgt tcttcgacct tctcccggaa tcgctcgcgc
     1141 tcacgagcgg cgcgttcgat acggggacgg tgacggccgt cgtcgctctg ggcttcctcg
     1201 cgtacatgct cctcgaccgc ctcgttctct tccatggcca ggccgaagcg catgagcacg
     1261 gtacgcacgc gcatcgcggc atcgtcggag cggggagtct ctcgttccat agtttcctcg
     1321 acggcatggc tatcggacta tcgttccagg tcgcgcccgc ggtcggcatc atcgtgacga
     1381 ccgcagtcct cgtccacgat ttctctgacg gcatcaacgc ggtcaatctc atcatgcgcc
     1441 atggcggaga gaggacccaa gcgctccgat ggctcatcac ggatgcagta gcgcccgtcc
     1501 tcggtatcgc cgccgcgagc ttcttcgcac tttccgaggt ggcgcttgga atgatgctcg
     1561 ccttgttcac gggattcttc ctctacatcg gggcggggga cctccttccg gagagtcacc
     1621 atgcgcaccc gaaggcgctc accacggtaa tgaccgccct cggcatcggc gtcatgtacg
     1681 tcgccgtttc gctcgcgggg gtgtagcgac agactccttc ccttacaggc accgtacact
     1741 gtcatttttt tggcaggatt cggtcacgag tcactaagcg tacgcggctc ttgcggcggc
     1801 aggattcttc cattacatgg actgttcagg ttgtatgatc ttttcttcga aaagctcgac
     1861 aacctctttg aatcctgcct gtccgtcctg tggacggacg ttcgccggag caaatgaaaa
     1921 gacgcccgtg aaggcgtctt ttcatttgct ccggcggcag gattcgaacc tgcgaccaag
     1981 tgattaacag tcacctgctc taccactgag ctacaccgga atgattgtgt gcgatgcacc
     2041 aaggattgag tggattgctt gtgttcggca ggtgccttac agtcaccctt cttatagaag
     2101 ctgttcaggt tgtatgacga tttctcccgt tggtcgaaat cctcgacaac ctcttgcgaa
     2161 agcttgcgct ttctcactct accactctgc tacaccggaa tgagaaggat agtggatgct
     2221 cacttccact agttcttcga atgctcggtg caagcgactg cttacaccgg aatgatgtcg
     2281 tcctcgtgaa gctccatttc gcgaggacat tcagcattgt actgaaaaaa cggctcaaag
     2341 caaatgtggt aaaatggtcg tatgcccgat ggcggtcgag agaaggagag catcatgagg
     2401 tcgttcacga cccgaagcgc acggcgtgcg tacgcgctcc ttagcctcat gaccgccatc
     2461 gtttttatcg gcgggttcgt cgcggtgagc cggaaggccg aggctcccgt ggccgaccta
     2521 tctaacaacg aaactcgtat ggcactcaca ctcacatcac cggcgttcga ggacggcggt
     2581 gtcattcctt ccgagtacac gtgcgacggc gcgaacgtga accccgaact ccgcataggg
     2641 ggcgtccccg aggggacacg ctcgctcgtc ctcgtcatgg acgaccccga cattcccgag
     2701 agcgtaaagc aggcgcgcgg catcgacgtc ttcgaccatt gggtcctcta cgacatacca
     2761 ccgacgaccg actccatccc gaagggcgcg ccggtcggca ccgccggcct caatggtctc
     2821 ggtgtcgccg gttacgccgg tccctgcccg ccggaccgcg agcaccgcta catattccga
     2881 ctgtacgccg tctcgggcac gctcgagttc gtgaaggcgc cgagcctcaa agaggtcgag
     2941 gatgccgcaa agggcatggc catcgagtcg gcgaccctca tcggccgcta caataggccg
     3001 gtgaacgtta ccaagtaaac cgaaaaccac catgaccacg ttatatgtga agacgggatg
     3061 tccctttagc gcgaaggcga taggcgccct cgacgcgtac ggggtcccgt acgagttgaa
     3121 gaacatcgcc gacccgggag tggcggacga gctcaccgag aagggaggca agctccaggt
     3181 accgttcctc gaagatggcg agacgtcgat gtacgaatcg gacggcatcg tcgcgtatgt
     3241 cgagtcgacg tacggaacgg gaagcgcgga gatcgagacg accgacggcg gcacgtgtcc
     3301 cgcatagaag gtatgtacgt acgggtccac gctctgccgg gagcgaagcg cgagcgggtg
     3361 acgagagacg acgagttcac gttcaccatc gcggtccgtg agccggccga gcgcaatctc
     3421 gcgaatcgac gcatcgccgc cgtcctcgca cgcgagttat cggttcggga gaacgatgtg
     3481 cggctcatca ccggccatcg ctcgtccgcg aagttgtatt cgataggaga gcgtcgttta
     3541 caagactagg acaatcgcac catgacattt ccacgcatca gcatgaagac gacgaacatc
     3601 accgtttcgc ccgatctcga agcgctcatc gagcataagt tcgaagcgct cggacgtctc
     3661 ctccccgacg ggaaggaggc gctctgcgag atagagctcg agaaggagac ggagcatcag
     3721 tcggggaaga tctaccgtgc cgaggtgaat ctcacggtcg cgggtaagct ctttaggacc
     3781 gaggcgaccg aagagcagat agagcacgcg atcgacgtgg cacgtggcga attgaaacgc
     3841 gagctcgaac gcgcgcacgg gaagcgccag agtctcttga aacgcggcgg gcaggcgatc
     3901 aagcgaatga tccgcttcgg tcgtgactag ctccggagga acgtctcata gtccatctct
     3961 tgttttcctt cgggtatcac cctcgtgatg acgagggaac cgtccgtgag gcgggcgtcg
     4021 acgatcttaa cgcggatcct ccgcccgcct ttttccgtga aaaaatacgc accgggccat
     4081 ccgtcgaacg cgcgtatctt ccgcaggttc tccgaggggt cggcggcgac gtctatctcg
     4141 cccatctcct tcgttatctt cttcgcgtac gtcgcgcggg attcatcttg tgcttctgcg
     4201 gtgagcgtgc ccgatatcca atcgggaagg atgcgcgcga ggagcgcacc gccgcgacgc
     4261 gcgaggagtt cgtcgagcgt acggccacgc gtcggccact cgctctcggg aatgcggact
     4321 tcctcccgcg cgacgacggg ccccgcgtcc atccgttcga cgggtaccat gatggagacg
     4381 cccgtcgggt tcacgttctc gaggatcgcc gagcgtatgg ggctcgcgcc gcgaagcgcg
     4441 gggaggagtg acgggtgtac gttcagcgcg ccgtagcgag ggagcgcgag gattgcgctc
     4501 gggatgcgct ttccgtacgc ggcgatgatg aaaagttcga cgccgctctt tcgcagtgag
     4561 gcgagcacgt cgtctgcgtc gagcgactcc ggctttaggc aggggatacc gcgttcgcgc
     4621 gcccatgccg cgaccggcgt ctcggttagg acgagctttc gtccgcgcgg tgcgtcagga
     4681 ttcgtgatga cgatggtggg cacgatgcca gccgcctcga gctcgtcgag cacgtagacg
     4741 ccgatgtccg gcgtaccgaa gaacgcgatg cgggggatat ccatatcagt cgttcgatgc
     4801 ttcgctcaac ttcttcgcct cgatgtcctc cttgttccag accttctccg ccttgtcgat
     4861 gaagaggata ccgtcgaggt ggtccacctc gtgctggaat atctgcgcga gaagacccga
     4921 ggcgccgcgc tcgtactcct cgccgttctc atcgagcgcg cgtagggtcg cgtgggtcga
     4981 gcgcttgacc gcgccgtagt ccttgccgac cgagaggcat ccctcgccga cgatgtgctt
     5041 gcgcttcgag agcttcacga ggaccggatt gatggcgacg agcgtcggga gcgtgtctct
     5101 atcctcgggg tccgtgctcg tattatggac gaggaagatg cggagcggca cgccgatctg
     5161 cggcgccgct atcgcgacgc cgttgaaccc cttcacgttg tacgtctcga gcgcggtgcg
     5221 catgtccttg aggaccttct tgatcttggg agacgatatc tcatcgcgcg gcacctccgc
     5281 cgctatctcg tggaggaccg gatgattttg attcactaat gtggccatat tctgcgatat
     5341 tcgccctact atagcgcacc tcggcgcgga aataaatgcg ctcgcctaca cgatcttatc
     5401 gggattcaag acgacgcgga ccgtgggcgg gagactacga agcagattcg agagcttgtc
     5461 gttcggccac gtgccccgcg gcagcttgat caaggcgtac atgatgggct cgagaagcgg
     5521 cgagaccggc gattggtaga tggacggcga gaaatccgcg aaccgggcgg cgatatcttg
     5581 ttcggttcgt ttcacgtcgt ccggcgtccc ttgccacgtg aggtgtacga aggtcgtgaa
     5641 gggcgggtac tcgaaggatc tccggagggc gagctcctcg gtgtagaaat gctcgaccga
     5701 accctgcttc gcctcgagaa gaacgtccgt cttcggcgcg cgggtctgca cgtacacggt
     5761 gccggtcgtc atctcgcgaa gcgagaggag gagcgcgagg ttctcctcgt cgagtttcca
     5821 cgtcggcgtc gagagaagcg cgtccatgtt catgacgacg gagaggtcga cgtccttcgt
     5881 gaggtagggg accgccatcg gcgtgccgag gagaatggcg cccttcgtct tgtagaacgt
     5941 atcgcgcagg aaggatgcct tcttgaacgt tctcgcgctc atgtggtcga agagaatggt
     6001 cggtgcgttc gggaagagct tatgaagctc gtcatagacc tgctgaatac cgatgccgcg
     6061 ttccttgaga cgccatgagc cgcactcgcg gcacgtgtcc tccgcccgga tgcgctcacc
     6121 ggaggaaccg cagacgaacc aacgtttctc gacgccgtcc ttcgtcgtgc ggaggagcga
     6181 gtacggtgcg ccgctctccg gtgaacggaa gatgtagccg caatcgacgc acgcgacgac
     6241 gggcgcgaga ccgcgccgag cggcgaagag gaacgtccgc ccgcggtgtt tccgcacgtc
     6301 ctttatcgct tcgatgaccg cgggagagaa gagacgaaac gcctctccct cggcacgcgc
     6361 catctcgacc acctcgagct ttccggggag ttcgatgcgt ttcggcgtct caccgtacgt
     6421 gtggtagatg tcgcgacggc gtgcctcctc gtcctcggtg cgcacgagaa ggtcggcgag
     6481 gatgacgttt cggcccgtgt ggcgcgcgtg ggcgaggagc gcgtcgcgat agtcgacgta
     6541 tggacgagaa agttccttgt agtagggcga gcgttcatgc tcgatgatga cgagcgtgac
     6601 gtcatggcgc tctatcatcg cgtacgaagg cgtcgcgatg atgagtttcg tcttcgagaa
     6661 gtcctcgagc gcctcgaacg aacgcttctg ctcggtcttc gcctgtgcgt tcgagaggag
     6721 gacgacgcgg tcgccgatac cctggccgag cgcgtcgcga agttcgtacg cgtcctgcga
     6781 ggacggcgcg acgaggagaa ccgagcccgc gtgcgcgaac gtctcccgaa cgagcgagcg
     6841 gaacgcgagg taccgttcac ggcggctcgc ctggagcacc gagaccgtgc cgcctgtggc
     6901 cggccctgga tggtgcgtat gggggagttt gacgtcgccg cttcgtatct cgggcgggag
     6961 caggtggtag agcatcgtgc cgagcggcgc gacgtacgtg cgtgagagtt ccttcgcggt
     7021 ctcgatgaac gcgttcccca acacggtcgc ctcggtttgc gccgggaggc ggcggagcga
     7081 gaacgtcgct gcacggagcg ccgtcttcgc ggcgctcacg ggcgcggtct ccgtgacgag
     7141 gccgagaatg gagccgtttc gaaagggtat cgtcacgatc gtccccggtt catagcggtc
     7201 ggtcccgaaa tacgagagcg cgtcgagcgc gataccgcgc ttgaggggga cgaccgtgat
     7261 gacgtacatg ccggtattct agcaaacccc ggcagttcgc ccaaaatcaa tactcttcga
     7321 ggtatgagac ctccgcgccg agcgaggtga ggcgctcgac gaggttttcg tagccgcgat
     7381 tgatcgaata gatgttccgg aggaccgagc ggcccttcgc gccgagcata ccgatgagaa
     7441 ggatggtcgc gggacgaagc gcgggcggac acacgagctc gttcggtcga agggtcgtgg
     7501 gtccggttat gtaaatgcgg tgcgggtcgg cgagaacggt ctccgcaccg agcttatcga
     7561 gctcggtgaa gtagagcgcg cgcttctcgt acacccaatc gtgaatgagc gtttgccctt
     7621 cggcttgcgt cgcgatgacc gcgaagaagg gaaggttatc gatattgagc ccggggtaga
     7681 ccgatgggtg tatcttctcg tgaagggcca tgagtcggga cggtttcgtc gtgatgtcga
     7741 cgagcttcgt gaggccgttc ttcgcgaggt acggcttcga cttcacgtac gtgaacccca
     7801 tcttctcgag tttgaggagc tcgagctcga ggaactcgat ggggcatcgc ttgatggtta
     7861 tctccgagcg cgtcacgatg gcagccgcga ggaagaacat cgtgtcggtc gggtcctcgg
     7921 agagcgtgta ctcgatgtcg cgtcgaagtt tgtcgacgcc ggtcaccgta agcgtcgtcg
     7981 tgccgacacc ctcgatcgta acgccgcaga cgcggagaaa cgcgcaaagc tcttgcacct
     8041 ggtagttcgc cgaggcgtac ttaatgacgg tggtcgatgg cgtgagcgca gcggccatga
     8101 ggacgttctc ggtcaccgtg tcgcccgatt catagaggac gatctcctcg ggacgcctgg
     8161 ctttgaccga gacgcggaac tcgtcctcgg tcgtgtctat tttcacgccg aagtgttcga
     8221 ggccgaagaa gtggggtcga accgtgcggc tgccgagttt gcatccgccc gactggggga
     8281 gcgaaaagga gcggaacgcg tgtatcaagg gaccgatgaa catgacgatg gaacgcgtct
     8341 ttcgcgcggc cgcggtgtcg atcgccttga gatcgagggt gcgaggcggc gtgatgatga
     8401 cgtcgtgctt gtcccactcg acgcgtacgc cgatcgattg gagcacctcg atgaggcggt
     8461 gtacctcctc gatgcgcggg acgttccgga gggtcgtcgt gccgcggttg aggagggagg
     8521 cgcagaggag gccgaccgcg ccgtttttgg aggtcgcggt cgtgatggtg cctttgagct
     8581 tccgaccacc ttcgatctcg aggttcacgg tgccgggaga gagcgtcacg aggttcttcc
     8641 taagcgcacg gccgagcttc gtgagcattt cggcgctcac gttctgcttc ccggattcga
     8701 ggcgcgcgac ggcgctctgc gtcgttttga ggcgcctcgc gagctcctcc tgggttatcc
     8761 cttgcgtctc gcggaggtcc gcgatatgga gtccgatgtt cgatttcatg cgcccaatat
     8821 agcatatatg ctatgtcagg gcgagccggc cgtgttgagt gccgcgccat tgtaaaaagc
     8881 cgcggacaca gtacacttag gcgacatgaa ggggttcact cccaagattg gcatcgatct
     8941 cggcacgacg aacgtgctcg tgttcgtcca tgggaagggt atcgtcctca acgaaccctc
     9001 ggtcgtcgcg gtctcggacc agaacgagat agtcgcggtc gggaacgagg cgaagctcat
     9061 gctcggccgc acgcccgaca ccatccgcgc gtaccgcccg atgaaggacg gcgtcattgc
     9121 agattaccga gtcaccgagg cgatgcttcg atacttcctc aagaaggcgc tcggtacgtt
     9181 caatatctgg agaccggacg tcctcgtctc cgtacccgcc ggcgtatcgt cgaccgagaa
     9241 gcgcgcggtc atcgaggcga ccctcaaggc aggcgcgcgg aacgtgtacg tcgtgaagga
     9301 accgattctc gccgcgatcg gagcgaaggt acccatctac gagccgcgcg gccacatgat
     9361 cgtcgacatc ggcggcggta cgatcgacgt cgccgtcatc tccttgggag gtatcgtcgc
     9421 gtcgaactcg gtcaaggtcg cggggaacaa gatagacgcc gcgctcatcg accatgcgaa
     9481 aaagaccctc aatctctcga tcggcgagaa gaccgcggag gacattaaga taaagatagg
     9541 ctcggcgctc cccctcgagg aggagctcgt cgtcgagata aagggacgtg acgtgctcac
     9601 cggcctcccg cggaccgcgg agatgaagac gaacgaggtc gttcgcgcca tctcgcgcga
     9661 gcttcgggac atggtgaagg cgatcaagga cgtcttccag ataaccccgc cggagctcgc
     9721 atcggacatc atcgaccacg gcatcatcat gaccggcggt acgtcccagc tccggaactt
     9781 ccccgagctc atcaagcggc gaaccggcgt gaaggccgtt ctcgcggacc aagcgtactt
     9841 ctgcgtcgcg aagggaaccg gcgaagcgct cgagcatctc gacacctaca agcggaccat
     9901 catggcgaag cgctagtcgg cagttgtgca ttctctcgtg ttccttcttt ctccgtttct
     9961 atttacaatt gaggcatgga gaccccaggg cgggagaaat tctcttcacc gcaggaagag
    10021 atcgcttttt tacgtaacga gatagcacga cgcgaacggg acctcctgtc gcgttcgaac
    10081 gaggtcgacc acaacgacgt cgaggcggtc ggccgtgagg tcattcgcga gtacggcgag
    10141 cacgaaccgg acctcctcct ccacaagagc cacgcgatcg acgcgtacga cctcgccgca
    10201 tcgagcgaga cggtgaagac cgcggccgag aaggtcgagg agatcatgcg ggtcgccgag
    10261 gcgcggggca tcagaaacgc gctcgcggtc ctcgaacgcg tgaaggaccc ctttttgacc
    10321 gatgaggtcc accgccacct cgtgagcagg ctccgtaccg agcggaaggt cgccgacctc
    10381 gagacggacg cgccgttgtg gaaggtttta gatatgacgc tctatgaggt cgcgcttcca
    10441 cgcgatccga acgatgagca tgagcactcg ctcaaagcca tcttcgccgg catggagcag
    10501 ttctatgcgg gcatgcagac catctcgcag ggaaaggacc cgcgttatta caccctcgag
    10561 atagcggtct ccgataaacg cgatgacatc atcttctacg tcgccgtgcc gaacgagttc
    10621 gcgaacctct tcgagaagca ggcactctcc ctcttcccgg cggcagagct cgtcctccag
    10681 cagaacgact acaatatctt cgtcgacggc ggcgagagca tgatcgcgat cgccgaagag
    10741 aagaagcacc atatctatcc cctgaagacg catgatgaat tcgaggtcga cccgctcacg
    10801 gtccttttga acgcgttctc gaagatcgag cgcgatggcg gcggcgcatc cgtgcagatc
    10861 gtcgttacgg gaggtgcgga gaagtaccat cgaacgtacg gaggcatcgt gcgaaggatg
    10921 cgaaacggtg aaccgacgga cgtcgcgata cggaagagca ccttgggcgg cgagctcttc
    10981 gaaggcgtga agagcctcat catgaactcg ccgaagaaga aggacgatga gcgtccacac
    11041 gagctcgaca cggagcgtat cgagctcttc aatcgaaaga tgtccgcgcc ggtcctcggc
    11101 atcaatatcc gcctcgcggt ctccgcgaag gacaagacgc gcgccgaaca gatactctcc
    11161 gagctcgagt cgagcttcca tcagttccag gacacgcagg ggaaccagct cgtcttcaag
    11221 cgactctcgg gcgtcgagaa ggccggtgaa ctgcgagcgt tctcgtttcg cgagctcgaa
    11281 tcgagccgca tcatcccctt atccatcacc gaactctcga ccatcatcca cttcccggcg
    11341 gaaggcgtca cgtcgtcgcc gcagttcaag cagtcgcgcg cgaagtacgc cgcggcgccc
    11401 gtcgatatgc cagaggtcgg cacgctcctc ggcgagaacg agttccgcgg cgcgaagcgt
    11461 cccgtgtatc tcacgcccat cgatcggctc cgccacttct acgtcatcgg tcagaccggc
    11521 acgggaaaga cgactctgct caagaacatg ctcatccaag acatcgagga cggcgccggc
    11581 gtttgcatga tagatcccca tggcacggac atcgtcgatg ttctttcggc catcccgccc
    11641 gagcgcatgg acgatctcgt gtacttcgat ccctcgaacc tcgatcgttc gctcggactc
    11701 aacatgctcg agtacgacga acggcatccg gaggagaaga cgttcgtcgt gaacgagctc
    11761 ttctccatct ttcagaagct ctacggcgcg aacccggagt ccatgggtcc gatgttcgag
    11821 cagtacttcc gaaacgcgac gctcctcgtc ctcgaggacc ccgcgtcggg ctcgacgctc
    11881 ctcgacatcg cgcgcgtcat gacggacggg aagtatcggg cgcggaagct cgcgcgcgcg
    11941 aagaacccgg tcgtcatcca gttctggacc gcgatcgcga cgaaggcggg cggggaggcc
    12001 gccctcgaga acatcgtccc gtacatcgtc tcgaagttcg acgtcttcac cgcgaacgac
    12061 tacatgcgcc ccatcatcgg ccagcagcgt tccgccttcg atttcagaaa gctcatggac
    12121 gagcggaaga tacttctcgt gaacctcgcg aaaggacgcc tcggcgagat taacgcgaac
    12181 ctcatcggca tgatcatcgt cgggaaactc ctcatggccg cgctctcgcg ggtcgacgac
    12241 ctcacgatga gctacccgcc gtactatctc tacatagacg agttccagaa cgtgacgacg
    12301 aactccatcg cctccatcct ctccgaggcg cgtaagtaca agctcggcct caccatcgcc
    12361 caccagttca tcgcgcaact cgacgaaccc atacgcgacg cggtcttcgg gaacgtcggc
    12421 tccttggcgg cgtttcgcgt cggtccggag gatgcggagt tcctcgagaa gcagttttcg
    12481 ccgaccttca ccgcgcagga cctcatgaac gtcgagaatc gaaacgcgta cgttcgcata
    12541 ctgtcaaacg gcatgccgac gccacccttt agtattcgca cgcttccgcc ccgagagacg
    12601 aaccacgagt acgcggcgga gatgatccgg tattcacacg cgcagaacgg gcgtccgcgc
    12661 gaggaggtcg agcgagagat acgcgaacgc tttctctcgt gagttcagtt atcaaccgaa
    12721 aatcgagttc ggggctctac cgagtgctac aatacgtgca atatgggagg gagtagtact
    12781 tcacagagtg ctgttttgcg tgacgaagac gcgggaaccg cgccctcctt cgacctgcgg
    12841 agttggcaag gtctcaccga ggtcctcaag cgtggaaagg agtcgctcgc cgacccgcac
    12901 gcgtacgcgg agttcaggaa tctcgtcctc tcgtacgcgc aacagggagg gaacgccgaa
    12961 cttcgtaagc agatagactc tatcgtcgcg tcgttcacga cgatcgcgag agacgaagca
    13021 tcactatccg aggagagcgc tcccgccccc gcacggaaga tcgtcgatcc gcctgtgacg
    13081 aagacggaag aggcgccgcc gtcggaggtc ccgccgaagc gtgtaggtat cggtatgcgg
    13141 cggctcgctc cgcgattctc cgcgtccgtc tcgaaaccga aaccggtagt tttgcctacg
    13201 aagacggagt cgacaccgcc gccccgacca gccgctccga aaccggaacc gactcccgtg
    13261 ccgcccgacg taacgacgcc gaaacctact acgccctcgt cgcccaaacc gatggtcgca
    13321 gagaagtcgc ccgcggagca ggcttctgaa cccgtctatg aaacgaagcc cgcgtccgga
    13381 gcgccacccg caccgatgtc agagccggcg cccacgtccg cttcgagcgt caaggaagca
    13441 tcgaccggcg cgtcgtcgtc ttttaagtcg ctcgacgagc acaaggcccg catcgcggag
    13501 ataaagcggc tcgtgaacgc gcagataggg aatccggcga ctctcatcga tacgcacaac
    13561 gagatcggca agcgatacat gaagtcgctc cttaatgccc tcaaagcgac gggtggtgga
    13621 ggggggaacg tcgatgattc gatggccgaa ctcgagtcgg ctttcgatgc gcttcgtaat
    13681 gacgctccgg aagcgaagag cggaaccgtt cccgcgatac cgcccgcccc ggagccgacg
    13741 cccgaacccg tcgccgagga ggtgccacgg agcgatacgc ctatgcccgc ggtgaaggag
    13801 gaagcgcccg agtgggacgc tcgcgccgac gccattcaga agaacgacga gaagatactc
    13861 tccgtcatgc gcgatatgca gaaggaccgc tccggtggcg aggtagccga ccttgcgtcg
    13921 gcgatcgcga acgggcgtac ggaggcggcg gtgaaagagg ccgtcgtggc ggaggtgcca
    13981 aaggcgccac gtgagccgga gccgatgaag gtcgcgtccg cgatggcgga ggcgccgcgg
    14041 aatagagtcg acttcgtccc gcagaacaag aacgtcgttc gcgaggtcgg caagcggccc
    14101 atcgaggacg tgaagaagga gaccggggtc gacccgcgcg aggtcgccat aaagcagacc
    14161 gagctctcgt cgcccgagat aaccgaggcg ctccatcagc ttctcaatga gtggagcatc
    14221 ttccgcggac gcggcttctt cggcatggga ccgtcagggc ccgagcatcc tctctaccac
    14281 acgctcgcac cgctctcgat gggcgaggtt atcgcgggtc gctgggaggg ctccgaccgg
    14341 aagctcacga agatgataaa gcagtacgtc gacgcatggc ggcacgagca gggcatcgcg
    14401 tacgtcatca ccgagacgtt cgagcactac ctgcggcgcg tggtccagcg catcatgaag
    14461 cgggggcagg ggggcggcgc gtagacgtcg tggtagaatc cgagggacac aagaagtgtc
    14521 ccgttttcat gcacgtacac cacgcacagc ttctcgtcgg ctcgctcgaa tgggcttcag
    14581 cattcgttcc cgagagcgag cggacgcccg gcaccgacgt cacggtcatc gataccgacc
    14641 tgctctcgat cgcgacggtg cgtgagctct cccgcgcggc ggcgcttcgg ccggtctcgc
    14701 gtccgtatcg gacgttcgtc gtcagtgcgc gcggggtccc gcatgaggcg cagaacgcgc
    14761 ttctcaagct tctcgaggaa ccgaacgaga acacgctctt ctacgtcatc gtcccgcgcg
    14821 aggacgtgct ccttgcgacg gtccgttcgc ggctccatct cgtcgcgacc gaggatagcg
    14881 cgacgtcttc tcacttcgag gccttcctcg gtctctcgca cgcgaagcga cttgcgatga
    14941 tcgccgaccg tgtcaccgcg gaggatgccc cctgggttgc cgatatactg cgcggtatgg
    15001 aggcgtacgc gcgaaagaga cgagacccgt ctctcatcga agagatcctc aaggtcgagt
    15061 cgtacctcgc cgggacggga gcctcgaaga agatgctcct cgagcacctc gccctttcgc
    15121 tttgagccgt cgggcgagta cggtacaatg cgaccatatg gcatacaatc cacagcaatt
    15181 cgtcgcgcga ggcgatgaga tagtgaagtg gctcgagagc gagttctcga gcgtccgaac
    15241 cggacgggcg acgcccgcgc ttctcgacct cgtcctcgtc gaatcgtacg gcgcgcgcgt
    15301 ccccatcgtg caggtcggct cggtcagcgt cgaggacccg cgcacgctcc gcgtcaccgt
    15361 gtgggataag acggcgataa aagcggtcga gaaggcgatc gtcgatgccg acctcggcgt
    15421 ttcggtcgtc gcagacggga gcggccttcg cgtcatcttc ccggaactca ctggcgaacg
    15481 gcgcgcacag ctcatcaaga tcgcgaaggc gaaactcgaa gaagcgcgcg tctccatacg
    15541 gggtgcgcgc gatgaggcgg tgaaggagct cgaagcgctc cagaagtctg gcgaactctc
    15601 cgaggacgag aagttctccg cgaaggagga tctccagaag cgcgtcgacg cgtacaatgc
    15661 gaagctcgtc gcggcgttcg aactgaagga agaggagatc aacgcataac ggcatgtcca
    15721 tactcatttt cctaggcgta ctcttcgcgc tcgttctcgt ccacgagctc ggacacttcg
    15781 cggtcgcgaa gtggacgggt atgcgcgtcg acgagttcgg tatcggcttt ccgccgcgtc
    15841 tcttcggcgt acggaagggc gagacggagt acacgctcaa tctctttccg ataggcggtt
    15901 tcgttcggat attcggagag aacggcgatg aggcgggcgg ggaagtgaac gacgacggtg
    15961 cgtcctccgt tccttccgac ggcggacggt ccttcacgga caaaagccgc tgggcgcagg
    16021 cggcggtcct cgtcgcgggc atcacgatga acatcctctt cgcgtggctc ctcatctcgg
    16081 cggcgttcgc gatgggcgtc gaaacgtcgg tcgacgagag cgaagcgggg ccgggcgccc
    16141 gcctcgtcgt ccttaacgta ctccccgaga gcccggcggc gtccgcgctc gacgcgggcg
    16201 acgtcatcga gagcgcctcc gccggaggag agagcatcga atcaccgacg ccttcggcgt
    16261 tcagcgagtt catcggccgc cacggcgatg aggcggttac gctcgcgtac cgaagaggga
    16321 gcgagcgcgg tatcgcggag gtgacgccga aggcgggtgt cgtaagcgct gaagcgtcgc
    16381 ggcccgcgat cggcgtgtcg ctttcgatgg tcgagaagga acgccgcgcc gtcggcgcgg
    16441 ccatcgtcga tgggttcacg tatacgttca cgagcctcaa ggagaccgcg ctcggcatcg
    16501 gcgggctcct ctatgacgcg attcgcggtc gcgctgatct ctcgcaagtc gcggggcccg
    16561 tcggtatcgt cgggctcgtc ggacaggcgt ccgcctacgg actctccgca cttctcatgt
    16621 tcacggcgtt catctccctc aacctcgcgg tcatcaatat actcccgttc ccggccttag
    16681 atggcgggag gctcctcttc gtcgccatcg agtcggtgaa aggttcgccg atcccggcac
    16741 gctacgcggg tatcgtgaac gcgatcggat tcgtccttct catcgcgctc atggtggtca
    16801 tcacggtcca tgacgtctcg aagctcatct gacgacccac atactataaa aaatcgcccc
    16861 ggcatactcc ggggcgtttt cgttcaggac gcgagcgttc gcataagctc atcgagcatc
    16921 tgtgcgtcga gaccgtcaag gagcgtgagc gtcggcgtgc gaatcgagta acagagcatc
    16981 tcgccgcgca tcgggtcgcc atggaggtac cgccagaacc gctctcccgg ctccgtgccg
    17041 atgtggagcc tcgcgccggt ctcgtcctct acgacgagaa cgaagatgcc gaaattgccg
    17101 aagctccgat cgaaacgcgc ttcgcgcatc gtgaagtcac gccgcttttt agaggttccg
    17161 ttctcgacga acgcggcctt ctgtgggtgc gtttccagta ccgcattgat cgtacgtatc
    17221 tcggacatcg ccctctcccg ttggaatctt gcggcagtct accatgggcg gtgtcgatgg
    17281 aatagggctc tgtatcgtct ctttttgacc ggcacttcct ttaaagatag aataaggagg
    17341 ttcgtacgag agaggccgat gtacctggag gagtttttca gtgtgtacga catttaacgt
    17401 catagtcatt ttgaacgcat gaagcaaagc cgcctgttca cgaagacccg gaaggaggtt
    17461 cccgcggacg aggtcgcgcg aaacgcacag ctcctcatac gcgcgggcta cgtcgcgaag
    17521 gagatggcgg gcgtgtacgc gtaccttcct ctcggacttc gcgttctcga gcgcatcaag
    17581 tctatcgtgc gcgaggagat ggacgcgatc ggcggacagg agctcatcat gacggcgctt
    17641 cagcggaagg aggtatggga gcgcacgaat cgttggagcg acgaggcggt cgacgtctgg
    17701 ttcaagtcgg agctcaaggc gggtggcgag gtcggtttcg gctggagtca cgaggaaccg
    17761 atcggcgaaa tgatgaagag ctacatcgcg agttacaaag acctcccggt ctacgtgtac
    17821 cagttccaga cgaaacttcg aaacgaactt cgcgcgaaga gcggcatcat gcgcggccgg
    17881 gagttcgtca tgaaggacat gtactcgttc tcggtgggcg aggaggacca cacggcgttc
    17941 tacgacgcga ccatcgaggc gtacaagcgc gtcttcgagc gcgtcggcat cggtgcggac
    18001 accttcgtca cgttcgcctc gggtggcgcg ttcacgcagt tcagtcatga gtttcagacc
    18061 attacggacg cgggcgagga cgtcatttac atacagaggg agaagggcat cgccatcaac
    18121 gcggaggtcc tgaacgatga cacgctccgc gaactcgggg tcacacgaga ggagcttacc
    18181 gaggtgaaga ccgcggaggt cgggaacatc ttcaacttcg gccgacagaa ggcggaggac
    18241 atcggactcg tctacaagaa cgaggcgggc gagagcgttc ccgtatggat gggttcctac
    18301 gggatcggcg tcacgcgcct catgggcgtc atcgtcgaga agttcgcgga cgagaagggc
    18361 atggtctggc cggaggcggt cgcgccgttc cccgttcacc tcatcgagct ctctggtcag
    18421 gatgccgaga taaaggagta cgcggacgaa ctctacagcg gtttcaagga ccacggcgtc
    18481 gaggtcctct acgacgaccg tgatgcgcgc gcgggcgaga agttcaacga tagcgacctc
    18541 atgggcattc cgtaccgcgt ggtcgtgagt cgaaagacga aggaggaggg taagtacgag
    18601 gtcgtgaacc ggcggaccgg cgaggtcgag cacctcgacg aggaatcgct ctacgagaag
    18661 ttcgtaaacg ctagggcggc atgagcaagt accccggcta catcgaccgc ctcatgggga
    18721 cgctctcgac ggacatcggc atcgacctcg ggacggcgaa caccttggta tacctcaaag
    18781 ggaagggcat cgtcctcaac gagcctacca tcgtcgccat caaccggaag acgggacagc
    18841 tcgtcgcggt cggaaacgac gcgaagacga tgctcggacg cacgcccgcg cacatcgagg
    18901 tcgtgcggcc cctcgtccac ggcgtcatct cggacttcga ggtgaccgag gagatgatcg
    18961 cgtatctcat caacaaggta cgctccgggt cgcgctctct catcgcgccg cgcgtcctca
    19021 tcggcgtgcc gtcgggtatc acgtcggtcg aaatgcgcgc ggcgcgcgac gccgcgaaga
    19081 acgccggcgc gcgctgggtg tacctcatcg aggaaccgat cgcggcggcg atcggcgcga
    19141 agctccccat ccataagccg caggggagca tgatcatcga catcggcggc ggcacgaccg
    19201 acatcgcggt gatgtcccta aacggcatcg tgacggcgcg gaaccttcgc gttgccggtg
    19261 accagctcaa tgaggacatc atggcgtaca tccgcgacca gttcaaagtg ctcgtcggcg
    19321 atcgtaccgc ggaggacacg aagatcgcgc tcgcctctct cgaggagtcc ggtgattcga
    19381 aggagctcat cgttcgcggg cgcgacgtga tgaccggcct tccgcgcgag gtcatcatca
    19441 ccgacagcga tatccgcgag gcgatcatgg ggtcgctcaa tcagatagtc gaggcgaccc
    19501 gcgacgtcat cgagctcacg ccgcccgaag tcctcgcgga cgtcatgcag cgcggcatca
    19561 cgctctgcgg cggcggggcg ctcataccgg gagtgccgaa gcttctcgag aacgtcctca
    19621 aactcccggt catcgtcgtt cccgacccct tgcgcgcggt catgcgcggc atcgggtcgg
    19681 tccttgaagg tctcgatatc tatgaagagt tacttatcga aaacgaggga gagctctctc
    19741 cgcagatctg atgcgggagg tcttcggcgc ctcgtcgcat ggatagtggt cgcgttcctc
    19801 gtgctccttc tcgccagagg accgcttggt gcgctcggct ccatggtcac gtcacctgcg
    19861 ttctcgtttc gacactggct cgagacgtca tccgcgacga tacccgtctt cgtacggagc
    19921 cgtcttgagc tcgaatccga gataaacgac cttcgttcga agctcgccgc ggaagagggc
    19981 aggaacgcgg cgttctcgtt cgtaacggac gagaaccgcg agctccgggc cctcttcgcg
    20041 tcgtcgtcct cgccgcgcat cctcgcgggc gtcatttcgc ggccgccgct tacgccgtac
    20101 gacaccctca tcatcgatcg cggagcggag gacggcgtca tcgaggacgc gccggtctat
    20161 ttcgggaatg accgcgccgt cgggtacgtt cgtaccgtgt tcagtgggag cgcgctcgtc
    20221 tccttgttct cgtcgccggg cgcggaatcg accgtgtacg tcttcggtcc gaacatcttc
    20281 acgacggcag agggtgaagg cggcggcgtc atccacctct ccgtgcccca gggcatcgaa
    20341 gtaaaggcgg gtgacgtcgt ggtactcccg tccctcgatg gtggcgtcct cggtctcgtc
    20401 gaccacgtga ccgccgagcc gaccgaaccc gagcaggacg cgtacgtcct ctacgacgta
    20461 ccgctccagt ccgttcgcct cgtcgcggtc gggacgacac cggttcgacc ggtctcgtgg
    20521 agcgaggcga cggacgaggt cgatcgtgca cgcgctgtct ttacgttcga tatcccatcg
    20581 acgacacccg agaagatcgc tccgtcaacg acaacgccat gacctccatg cgcatcatcg
    20641 ctttcatttg tctgttcttc gcggctctgt acctgccgct ctggctcttc gcgctcggcg
    20701 ccgtcgcata cgcgttcacg ttccgcgcgt acgagcttct cgtcctcgcg gtcctcatcg
    20761 atgccgagtt cggcgagccg ggtacgggag cgtaccgtta cacggcgtcg gtcgcgatcg
    20821 cgctcctctt cgcggcgctc gtacgaccgt atcttcgttt ctacgcatga gattcttcag
    20881 gaaaaaaaga cgagccgaga tatcgttcga tgagatcctt ctcgattcat cgaaccttcc
    20941 gtcgttcaac acgggacgct tggaggggcg catcgagctt ccgctccaga agcggaacgt
    21001 gtacgtcgtc gggggtatct tcgcggtcat cgcgctcgtc ttcttctcgc aactcgtcaa
    21061 tcttcaggtc attcagggag cggaactgaa cgcgaagagc gagagcaacc gactcgacca
    21121 gagcatcatc atcgctgaac gtggcatcat ctatgaccgc tatggcgagc tcctcgcctg
    21181 gaacgaacgc gacgcggcgg ggaagtacga ttttccggtg cgggcgtaca ccgaccgccg
    21241 cgggttcggc cagcttctcg ggtacgtgag ctacccgcag cgagataagg ccggtttcta
    21301 ctaccgtacc gagtacatag gacgtaacgg cgtcgaaagc gcgtacgacg acgtgctccg
    21361 gggagagaac ggacggcacc tcatcgagga gaacgtccac ggtgacacta tctccgagaa
    21421 cgcggtcgat gagccggtcc ctgggaaacc gctctccctc gcgctcgacg cggagctctc
    21481 ggaggtcatg tacgacctca tcgcgtcgac gacggcagag aacgggttta gaagcggcgc
    21541 cgcggcgatg atggacgtcg ataccggcga gatcgtggcg ctcgcgagct tcccgtcgta
    21601 cgacccggag gtcctcgcag acggcgacga cgtcgcgaag atcgaggagt acaacgccga
    21661 taagcggttt ccgttcctcg acaaggtgat gggcggcgcg tacgtccccg gctccatcgt
    21721 gaagcccatc gtcgcctacg gcgcgctcgc cgagcacatc atcagtcccg acaaggtcat
    21781 cgtgtcgacg ggctcgctta ccattccgaa tccgtacgac ccgtcgcatc ccgccgtcta
    21841 ccgcgactgg aatccgtcct tgggcgcgat gaccatccgc gaggggctcg cgttctcgtc
    21901 gaacgtcttt ctcatgacga tcggcggcgg cttcggcgac cagaagggcc tcggcatagc
    21961 gaagctcgac aagtactacc gcctcttcgg actcggacag aggacgggcg tccccctcga
    22021 cggcgaacaa gccggtgtcg tccccgaccc cgtatggaag gagaaggtct tcaaggacga
    22081 gtggcgcctc ggcgacacgt acctcacgtc gatcggtcag ttcggcttcc aggtgacgcc
    22141 gctccagatg cttcgagcgt acgcggcgat cgcgaacggc gggaagctcg tgacgccgca
    22201 ggtcgtctta ggggcagagg gagacaccgt cgacctccat ctcgaccaaa gcgctctcac
    22261 ggtcgtgaag gaaggcatgc gcgaggccgt cacgaaggtc ggcggtaccg cgcgcgccct
    22321 cgatcggaag gatgtcatgg tagcggcgaa gtcggggacc gcagagctcg gcacgtcgaa
    22381 ggcgtacgtg aactcgtggg tcgccggatt cttcccgtac gagcacccca agtacgtatt
    22441 catccttctc atggagtacg gtccgcgcac gaacacggtc ggctcgagct ccgtcatggg
    22501 taaggtgttc gagtggatgg caacgcaccg gaaggagtat ttcggtgcct aaaatcgtca
    22561 agttgacttc aaaggtggcc tacgtataat tgactcacta attttatgca ccatgaatga
    22621 accacatgac tcgttcctta ctcaggagaa gttcgaggag ctgaagaagg aactctacca
    22681 tctcaaaacg gttcgccgaa aggaggtagc ggagtcattg gagtacgcgc ggtcgctcgg
    22741 cgacatctcg gagaacgccg agtaccagga ggcgcgcgac atgcaggccg cgatcgagga
    22801 gcgcatccag tacctcgaga aggtcatcaa ggaggcgaag atcgtcgcga cgacgaagaa
    22861 gggcgacgtc gtcgccttgg gctccgaggt caccatccag aaggagaaga cgaacgaggc
    22921 gcacacgtac acgatcgtcg gttccgagga ggcgaacatt cacgagcgga agctctcgtt
    22981 cctctcgccc ttaggcgagg cgctcatggg gaaggcgaag ggtgacacgt tcaccttcga
    23041 gacgccgaac ggcaagcagt cgtacacggt cacgaaagtg gcgtagaaca tagaaaaagc
    23101 atcctctacg gatgcttttt ccgttctacg agccggagcg atgtttcgcc gaccacacgg
    23161 aggcgaaacc gcgaggtcgg gcaggaggta tttgtgggcg tagcgaacta gtaacctgac
    23221 gctagagatt cgaaaaaaca tcctctacgg atgctttttt cgtccgaccg gggaacgttg
    23281 catttgcgcg cgttcacggt actatgattt tcatgtcagc actggaagag atacgaaacg
    23341 agcgcctcaa aaagctcgag ctcctcaagg agaagggaat agacccctac cgcgcgacga
    23401 cgacgcggac ccacacgatc gcagaggccg acggtcgatt cgacgagctc gccgcaagcg
    23461 gcgagcaagt cacgctcgcc ggacgcgtga tggtcgttcg cggtcagggc gccatcctct
    23521 ttgcggacct ccaagacggc acggggaagt tccaggcggt cgtgaaggag gacacgctcg
    23581 ccgcggattc cttcgcgctc ttccgcgacg ctgtc
//
LOCUS       DASTSP010000048         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_547570, whole genome shotgun sequence.
ACCESSION   DASTSP010000048 DASTSP010000000
VERSION     DASTSP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547570"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFS75_02285"
     CDS             <1..692
                     /locus_tag="VFS75_02285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4677523.1"
                     /translation="SNYTRNIVVERYGVDPKKVEVVHNGIKPGRPRRLPPALAELKRA
                     GKKIVFYNGRITIQKGVDYFVRAARRVVDVDPSVVFVISGWGDMERQIMHLVGSLGLS
                     ENVIFAGALWDEERDRMYQAADLLVMPSVSEPFGLVPLEAIQHGTPALISKQSGVAEV
                     LTHALKVDFWDVDEMANKILAALRYPRMSRQLVEEGQRELHRVTWRRAAEKVISLYHR
                     LLSWWREGRMG"
     gene            707..2032
                     /locus_tag="VFS75_02290"
     CDS             707..2032
                     /locus_tag="VFS75_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEU4677524.1"
                     /translation="MLSVCPYFHVHQPHRVKRYRVFDIGRDGEYFNAKDDTDLNNEKV
                     LYKVAEKSYLPATAVLGDLLARHPEFRFAFSFSGTVLDQFEEYAPHVLKRFQELVASG
                     RVEILADTYYHSLAFFYSPREFERQVAHHAKRVKELFGVTPRVFRNTELSYTNDLARW
                     CDERGYLGIMAEGWDPILGWRSPNYVYRPVGTRNIKVLLKNYKLSDDIAFRFGNRSWE
                     SWPLTAETYAHWVHAHHGDGHTINLFMDFETFGEHQWEDTGIFDFLHELPNRLLSHGE
                     TTFKTPTETILSYDAVGDFDVPHILTWADTDRDLTAWVGNDIQRDAIASIYGMEDDVM
                     RTGDSGLIETWRRLQTSDHFYYMCTKWFNDGDVHAYFSPYESPYDAYIAFMNALSDLQ
                     LRVKEALRRSTERIEAPRPSLWERFISWLRRLFGKLAFIIRRRTGVRTP"
     gene            2053..>2343
                     /locus_tag="VFS75_02295"
     CDS             2053..>2343
                     /locus_tag="VFS75_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677525.1"
                     /translation="MTTIAKKGGKKTGAKRDLKKAKRVLARAEGAQCFWVTNGAIIST
                     LVDLSNLLGEMETSVYRYHVTKDKNDFADWVEFVLGDRELALALRKAKQPKSA"
BASE COUNT          467 a          732 c          705 g          439 t
ORIGIN      
        1 taagcaacta cacgcggaac atcgtcgtcg agcgctacgg cgtcgacccg aagaaggtgg
       61 aggtcgttca caacggcatc aagccgggcc gtccgcgtcg gcttccgccc gccctcgcgg
      121 aattgaagcg agccgggaag aagatcgtct tctataacgg ccgcatcacc attcaaaagg
      181 gcgtcgacta cttcgttcgc gcggcgcggc gcgtcgtcga cgtcgacccg tcggtcgtct
      241 tcgtcatctc gggatggggt gacatggagc gacagatcat gcacctcgtc ggctctctcg
      301 gtctctcgga gaacgtcatc ttcgcgggcg ccctctggga cgaggagcgc gaccgcatgt
      361 accaagcggc ggacctcctc gtcatgccat cggtctccga gccgttcggt ctcgtgcctc
      421 tcgaggcgat ccagcacggg acgccggctc tcatctcgaa gcaatccgga gtcgcggagg
      481 ttctcacgca cgcgctcaag gtcgattttt gggacgtcga cgagatggcg aacaagatac
      541 tcgcggcgct ccgctacccg cggatgagcc gccagctcgt cgaagaggga cagcgcgagc
      601 tccaccgcgt gacgtggcgt cgcgccgcgg agaaggtcat ctcgctctac catcgcctcc
      661 tctcgtggtg gcgagagggg agaatggggt agaatgaaca tacatcatgc ttagcgtttg
      721 tccgtatttt cacgtccatc agccgcaccg cgtgaagcgg taccgggtct tcgacatcgg
      781 acgagacggc gagtatttca acgcgaagga cgatacggat ctcaacaacg agaaggtgct
      841 ctacaaggtc gccgagaagt cgtaccttcc ggcgacggcg gtcctcggcg acctcctcgc
      901 gcggcatccc gagttccgct tcgcgttctc cttctcggga accgtcctcg accagttcga
      961 ggagtacgcg ccccatgtcc tcaagcgatt ccaggagctc gtcgcctccg gccgcgtcga
     1021 gatactcgcc gacacgtact accactcgct cgcgttcttc tactcgcccc gtgagttcga
     1081 gcggcaggtc gcgcaccacg cgaagcgcgt gaaagagctc ttcggcgtca cgccgcgcgt
     1141 gtttcggaac accgaactct cgtacacgaa cgacctcgcg cgttggtgcg atgagcgcgg
     1201 ctacctcggc atcatggcgg aaggctggga cccgatactc ggctggcgga gcccgaacta
     1261 cgtgtatcgc cccgtcggca cgcggaacat caaggtcctc ctcaagaact acaagctctc
     1321 ggacgacatc gcgttccgtt tcgggaatcg ctcgtgggag agttggccct taaccgcgga
     1381 gacgtacgcg cactgggtgc acgcgcacca cggcgacggg cacacgatca atctcttcat
     1441 ggacttcgag accttcggcg aacatcaatg ggaggacacc ggcatcttcg acttccttca
     1501 cgagcttccg aaccggctcc tctctcatgg tgagacgacc ttcaagacgc cgaccgagac
     1561 catcctttcg tacgacgcgg tcggcgactt cgacgtgccc cacatcctca cgtgggcgga
     1621 caccgatcgc gacctcaccg cgtgggtcgg gaacgacatc cagcgcgacg cgatcgcctc
     1681 gatctacggc atggaggacg acgtcatgcg aaccggcgac agcggcctca tcgagacgtg
     1741 gcgtcgactt cagacgtcag accacttcta ctacatgtgc acgaaatggt tcaacgatgg
     1801 ggacgtgcac gcgtacttca gtccgtacga atccccatac gatgcgtata tcgctttcat
     1861 gaacgccctc tctgacttac aattgagggt gaaggaagca ttgcggaggt cgaccgagcg
     1921 aatcgaagcg ccgcgtccga gtctatggga acgtttcatc tcgtggctcc ggcgcctctt
     1981 tggcaaactc gcgttcatta tcaggcggcg cacgggcgtt cgtacgccgt aacagttcat
     2041 cagttcttat ttatgacaac aatagcgaag aaggggggga agaagaccgg ggcgaaacga
     2101 gatctcaaga aggctaagcg cgtcctcgcc cgtgccgagg gggcgcagtg tttttgggtg
     2161 acgaacggcg cgatcatctc gacgctcgtc gacctctcga acctcctcgg agagatggag
     2221 acgtccgtgt accgatacca cgtcacgaag gataagaacg acttcgccga ttgggtggag
     2281 ttcgtgcttg gcgataggga gctcgctctc gcccttcgga aggcgaaaca gccgaagagc
     2341 gcg
//
LOCUS       DASTSP010000049         4961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_772959, whole genome shotgun sequence.
ACCESSION   DASTSP010000049 DASTSP010000000
VERSION     DASTSP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772959"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1055
                     /gene="obgE"
                     /locus_tag="VFS75_02300"
     CDS             75..1055
                     /gene="obgE"
                     /locus_tag="VFS75_02300"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU4677526.1"
                     /translation="MFVDELTIDAKAGNGGDGVVRWRREKFVDRGGPAGGNGGKGGDV
                     YMRAVRDLSLLSKYTGEKTFRAEDGRPGEKRSKYGKDGEDLYIDVPVGATVTEHERER
                     VYTFTKEGETHKVLLGGRGGLGNEYFKSSTNRSPEESTKGKRGEAGRFTIVLALMVDG
                     GLVGVPNAGKSTLLNALTNTRARVGAYPFTTTEPHLGELYGYVLADIPGLIEGASLGK
                     GLGHKFLRHVSRTKVIIHCVSLAEDEPAVTYGLIRSELGKYSDALLEKPEWIVLTKAD
                     LASPEKIAETETRLRGVTTAPIAVVSAETGEGIKELSDSLTHFFKKGMDQ"
     gene            1241..2641
                     /gene="gatB"
                     /locus_tag="VFS75_02305"
     CDS             1241..2641
                     /gene="gatB"
                     /locus_tag="VFS75_02305"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU4677527.1"
                     /translation="MKTYTPTIGIEVHAELKTRTKMFCDSKNEPHDAEPNTNVCPICM
                     GHPGTLPTINRQAVEHVLKVGMALGSELASYTEFDRKNYFYPDIPKGYQISQYEYPLV
                     KGGTLAGVAITRVHLEEDTARSLHDQGEGSVIDYNRAGVPLMELVTEPVIHDAKAAGD
                     FARELQLLLRTLGVSDANMERGEMRVEANISVSDSDTFGTKVEVKNLNSFKSVEGAIE
                     YEVRRQIEAIERGEQVIQETRGWDEAKGKTYSQRVKESAHDYRYFPEPDLPKLSVDEA
                     LLSRIRESMPELPAQKREKYSNLGLTTEQISVIISDAAVDSFFSRCVESAESDGDLVR
                     LSANYLVTDLLTLVDDTGRLDKAHADLFFALMRMVHDSRVTSRVAKDILEEVVIDGAD
                     PEVIAKDRGLLQSSSDEALLPIVDEVIGANKTVADDFEAGKEAALQYLVGQGMKLSRG
                     SANPKVLADLFRKRLG"
     gene            2715..3701
                     /locus_tag="VFS75_02310"
     CDS             2715..3701
                     /locus_tag="VFS75_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677528.1"
                     /translation="MEVLVLDGKNYVKASKAARDLGYATDYVGQLCRSGKVDARLIGR
                     TWYVDPDQLGHHRVEKKRMSRVKAREQAKKAIEEVHRLRVNDTTPKPHFTAGIRYEPD
                     DRELIPETRKLSVESLGLVKVHPDEVEGNELTIENEGEKILMSGDLTVEDVSDDPVEV
                     DTVVLTPRVVRRTRKEVLAEERKRLKEEREAAKEAEETRFIDRIASSTDDDGQESTVV
                     PINAVETRTEPQEGLGALADVADATDELQADFEPEPSVERVGWGARVAVLLATIALVA
                     LTMPVTKLMYYSGETDTSVISYTYEPQEAFGNLWQRIMESRVHDIIGTRIKW"
     gene            4065..4793
                     /locus_tag="VFS75_02315"
     CDS             4065..4793
                     /locus_tag="VFS75_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677529.1"
                     /translation="MSNLLNVEGKEFTTATAAAKQLGYTKDYLLLLIKDGKVEGRKIG
                     NKWFVHVPSAERFFRQAAISKVVRRRRISLERKSELERHVRARVGGSHKAAVLETLAI
                     LIIGLSLGATGYLGSVSETASAGSSLAFLERLAISFHGLFAPEEVRTDFGNTDIAVPT
                     TTATTSVATERPSPAMIVAPAESFTEDSVDALKESFSDEVNVTVDPNDSHTGIIVPVF
                     KSGPGEPYRFLLVPMASASSSETQ"
BASE COUNT         1160 a         1350 c         1500 g          951 t
ORIGIN      
        1 ggtgtagcat cactcatcat gctcgcggtc tcgtggcgac aagcccgaaa gggactccga
       61 gaagaatagc aactatgttc gtagacgagc tcacaatcga cgcgaaggcg ggtaacggag
      121 gcgacggcgt cgtcaggtgg cgtcgtgaga agttcgtcga ccgcggcggc ccggcgggag
      181 ggaacggcgg aaaggggggc gacgtctaca tgcgggcggt acgcgacctt tcgctccttt
      241 cgaagtacac gggcgagaag acgtttcggg cggaagacgg ccggcctggt gaaaaacgca
      301 gtaaatacgg caaggatgga gaggatctct atatcgacgt gccggtcggc gcgacggtga
      361 ccgaacacga gcgggagcgc gtctacacct tcacgaagga gggtgagact cacaaggtgc
      421 tcctcggcgg acgtggcggc cttggaaacg agtattttaa gtcctcgacg aatcgttctc
      481 ccgaggagtc gacgaaggga aagaggggcg aagcaggccg attcaccatc gttctcgctc
      541 tcatggtgga cggtggtctc gtgggcgtcc ccaatgccgg gaagtccacg ctcctcaacg
      601 cgctcacgaa cacgcgcgcg agagtgggcg catatccctt cacgaccacc gagccgcact
      661 tgggcgaact ctacgggtac gtgctcgcgg acatccccgg gctcatcgaa ggggcctcgc
      721 tcggtaaggg tctcggccac aagttcctcc ggcatgtatc gcggacgaag gtcatcatcc
      781 actgcgtctc cctcgcagaa gacgagcccg cggtgacgta tggtctcata cgttccgagc
      841 tcggcaagta cagcgatgca ctactcgaaa agccggagtg gatcgtcctc acgaaggccg
      901 atctcgcgtc ccctgaaaag atagcggaga ccgaaacgcg gcttcgcgga gtgacgaccg
      961 ctccgatcgc ggtcgtgtcg gcggagacag gagagggaat taaggagttg agcgactctc
     1021 tcacgcactt tttcaagaag ggaatggatc aataaagacg tcgaacaggt cactttctat
     1081 tgacagaaat gtctttcgtg ttagtgaaat atacatatgc acggcttaac caagccatat
     1141 atgtaattca ttcggctgat tgccttaaaa tcgttgacaa tattgtcttt cgtgtcttta
     1201 ttgcttgtct ctatgagggg acaacggtac tattacgccc atgaaaacgt acacgccgac
     1261 catcggaatc gaggtccatg cggaacttaa gacgcggacg aagatgttct gcgactcgaa
     1321 gaacgagccg catgacgcgg agccgaacac gaacgtctgt cccatctgta tgggacaccc
     1381 aggcacgctc ccgaccatca accgtcaggc ggtcgagcat gtactcaagg tgggtatggc
     1441 gctcggcagc gaactcgcgt cgtacaccga gtttgatcgg aagaactatt tctacccgga
     1501 catcccgaag gggtaccaga taagtcagta cgagtacccg ctcgtgaagg gcggtacgct
     1561 cgcgggcgtg gcgatcacgc gcgtccacct cgaagaggac accgcccggt cgctccatga
     1621 ccagggggag ggaagcgtca tcgactataa tcgcgcgggc gtccccctta tggagctcgt
     1681 gacggaaccg gtcatccacg atgcgaaggc tgcgggcgac ttcgcacgag aactacagct
     1741 cctccttcgt accctcgggg tatccgatgc caacatggag cggggagaga tgcgagtaga
     1801 ggcgaacata tcggtaagcg attcggacac gttcggcacg aaggtcgagg tgaagaatct
     1861 caactcattt aagtccgtcg agggtgcgat cgagtacgag gtaaggcggc agatcgaggc
     1921 catcgaacgc ggagagcagg tgatccagga gacgaggggt tgggacgagg ctaaaggcaa
     1981 gacatatagt cagcgagtta aggagtcagc gcatgattat cgttacttcc cggagccgga
     2041 cctcccgaaa ctctctgttg atgaggctct cttatctcgg atacgcgagt cgatgcctga
     2101 attaccggca cagaagcgcg aaaaatatag taatctcgga ttaacgactg aacaaatatc
     2161 ggtcatcatc agcgatgccg ccgttgattc gttcttctcg cgatgcgtgg aaagcgcgga
     2221 aagtgatggt gatctcgttc gattgtccgc gaattatctc gtcaccgacc tgctcacgct
     2281 cgtggacgac accgggaggc tcgataaggc acacgccgac ctgttcttcg ccctcatgcg
     2341 catggttcac gattcacggg tcacttctcg tgttgcgaag gacattctcg aggaggtcgt
     2401 gatcgatggc gcagatcctg aagtgatcgc aaaggaccgc ggacttctcc agtcgagttc
     2461 agatgaagcc ttgctcccga tcgttgacga ggtcatcggc gcaaataaga ccgttgccga
     2521 tgatttcgag gcgggtaagg aggctgctct ccagtacctc gttgggcagg gaatgaaact
     2581 ctctcgcgga agtgctaacc cgaaggtgct tgcggacttg tttaggaagc ggctcggcta
     2641 ggcgcgctcg cacagccgat atctcctttg ataccactat acagcgccat aggcgggtgt
     2701 atactgtagg gaatatggag gtccttgttc ttgatggaaa gaactacgtg aaagcgagca
     2761 aggccgctcg agatctcggc tatgcgaccg attacgtcgg ccaactctgc cggagcggca
     2821 aggtcgatgc gcgcctcata ggccgtacat ggtacgtcga cccggaccag ctcggacacc
     2881 atcgcgtcga aaagaagcgc atgtctcgcg ttaaggctcg cgagcaggcg aagaaggcga
     2941 tagaggaggt gcatcgcctc cgcgtcaacg atacgacgcc gaagccacat ttcaccgcgg
     3001 gaatacgcta cgagccggat gatcgggagc tcataccaga aacccgaaaa ttaagcgtcg
     3061 aatcgctcgg ccttgtgaag gtccatccgg atgaggtcga agggaacgag ctcaccatcg
     3121 agaacgaggg ggaaaagata ctcatgtcgg gcgacctgac ggtcgaagac gtgtcagatg
     3181 accccgtgga ggtcgatacc gtcgtactca cgccgcgcgt cgtccggagg acgaggaagg
     3241 aagtgctcgc cgaagagcga aagcgtctca aagaggagcg cgaagcagct aaagaggcag
     3301 aagagacgcg tttcatcgat aggatcgcgt cctcaacgga cgatgacggg caagaaagta
     3361 ccgtagtgcc gataaacgcc gtagaaacgc gtacagagcc tcaggaaggc ctaggagctc
     3421 tcgcagatgt ggcagatgct acagatgaac ttcaagcgga ttttgaacca gagccctcgg
     3481 tagagcgagt gggttggggc gcgcgggtcg ccgttcttct cgcgaccatc gcgctcgtcg
     3541 cactcaccat gccggtcacg aaactcatgt actactccgg agagaccgat acatccgtta
     3601 tctcgtatac ctacgagccg caagaagcat tcggtaatct gtggcagaga ataatggaat
     3661 ctcgggtaca cgatattata ggaacacgaa taaaatggtg accagatagt aacccagaga
     3721 gccgtctcga gcggcttttt tcctacttcg cgcagtaacg gggtacagta acggcgaatc
     3781 tggtcgccgc gctgatctat gccctaactc cctcgctcgg accagcgcgt aattcacggt
     3841 gaatcgccgt catcgagcag agccggatgg tctgtcgatg ccagccattg gagacgagcg
     3901 cacgagcgcc tcgtgctcac cgccacgtcg gaaattgtgg tatccgagcg ggggagagca
     3961 ccgccgaaat taggcgaaaa agttatcccc gcgcggggcg atttttcgtg cgagaaaaac
     4021 gcgaaaaaac tttataattt ataggtattt cagtcatttc atagatgtcg aatttactca
     4081 acgtagaggg taaagaattc acgacggcga cggcagcggc aaagcaactc gggtacacca
     4141 aagactacct cctgttgctc atcaaggacg gcaaagtcga aggtcgcaag atcgggaaca
     4201 agtggttcgt gcacgttcca tcggccgaac ggttttttcg acaggcagct atctcgaagg
     4261 tagtgcggcg acggaggata tcactcgagc gaaagtccga gctcgagcgt catgtccgtg
     4321 ctcgggtcgg cgggagccat aaagcggcgg tcctcgagac gctcgcgatc ctcatcatcg
     4381 gtctctcgct cggcgcgacc ggctaccttg gcagtgtctc ggagaccgcg tccgctggga
     4441 gctctctcgc gttcctggaa cgactcgcga tctcgtttca tggactcttc gcaccggagg
     4501 aagtgcggac ggatttcggg aacaccgaca tcgcggttcc gacgacgacc gcgacgacgt
     4561 cggtcgcgac ggagcgcccg tcgccagcca tgatcgtcgc acccgcggag tcgttcaccg
     4621 aggattccgt cgatgcgctc aaggaatcgt tctccgacga ggtgaacgtg accgtcgatc
     4681 cgaacgactc gcataccggc atcatcgttc ccgttttcaa gagcgggccc ggagaaccat
     4741 accggttcct cttggtgcct atggcgagcg catcgtcgtc tgagacgcaa taaattttca
     4801 tgcagttcgt tatccgaaaa ccgcacaccc tcgcactcct cctcggagtc gcgttcgtgc
     4861 tcgcgcagtt ttcgggtgcg gcgcacgcgt cggccgcagc gaactacgag ctcaattacc
     4921 aaggcaagct caccgatagc tcgaacgccg cggtcgcgga c
//
LOCUS       DASTSP010000050         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_772983, whole genome shotgun sequence.
ACCESSION   DASTSP010000050 DASTSP010000000
VERSION     DASTSP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772983"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..>2771)
                     /locus_tag="VFS75_02320"
     CDS             complement(181..>2771)
                     /locus_tag="VFS75_02320"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677530.1"
                     /translation="TVYCTDTTNTCNPVTSGTAVTVSTESATNYVRWKSTDNAGNVQT
                     VQSSGAIKIDKSAPGTSGSVTGGSAGTNGWYTSDVTYSLSTIDVLSGIASTVYCTDTT
                     NTCNPVTSGTAVTVSTESATNYVRWKSTDNAGNVQTVQSSGAIKIDKTAPTVPTPSDD
                     GDYVSTTTLTFTGAPSDALAGLSSCSAQVDVGDTDGLSLAMGTTSVGTDGDHTFTGEN
                     DGTYYYRYSCTDAAGNGSSYSSWSDGVTVSIHSLSLVSNGGTLGGTAAGQKDYGTAIT
                     ITQTPPSGYTFGGWSSTNDVCTGIGDCSFSMPAFDVTVTATYTGLPFFTSADDQTFTS
                     GQSAAGISTITITDPAGDISASTDIRIAIATSSVDMRFDSSDTSATFGGTASDKVSNP
                     VSYEGDDSVLVISVDVNFLAGDTLTVSGLSFTSFGTANSATTALSMLIHGSGSTTAGY
                     DAKKVTIEGGLTFGAHSEGQVSDQFSTGSITDEPLFAFSLAPHGESIEVESLAFELSS
                     VKGVGENDITNVGLYRDNDSDRTHSGPDAAVGGAPTVSVENGAGAISFGEPFTATTTN
                     DYILTGSVANLSGSDRLYVAMNTGDVTAVGAVSGAVDKSGSAPGVLHALARVGAVGGG
                     GGATALGENFTPVVATTTSGGSDGGSGGAIDPNSGTTIGSEAGYFPPAGNGTPHNGWT
                     AGGNAYASDGSYVTTSGSGVQQSYGTFNFTVPQNDAMTGIGVKLEASGSSASGTIGVK
                     LSWDGGSSWTFEKTTSVLTQLDAVYTVGGQGDTWSRSWSPSETTNGNLLVDVIAHPGN
                     NTISLDAIQVNVYHQATGGGQGGGGAVFGNETRFFANAYEAFTSAFGLRVETFVNWLF
                     GFAHGF"
BASE COUNT          464 a          853 c          904 g          550 t
ORIGIN      
        1 gtccgcgacc gcggcgttcg agctatcggt gagcttgcct tggtaattga gctcgtagtt
       61 cgctgcggcc gacgcgtgcg ccgcacccga aaactgcgcg agcacgaacg cgactccgag
      121 gaggagtgcg agggtgtgcg ggtttttgag taagcggtgc atggtcgttt gtgtatcgtc
      181 ctagaatccg tgagcgaaac cgaagagcca gttgacgaag gtctctaccc tcaagccgaa
      241 ggcgctcgtg aacgcctcgt acgcgttcgc gaagaagcgg gtctcgttgc cgaagacggc
      301 gccgccgcct ccctgcccac cgcccgtcgc ctggtggtag acgttcacct gaatggcgtc
      361 gagacttatc gtgttgttcc ctgggtgtgc gatgacgtcg acgagaagat tcccgttggt
      421 cgtttccgaa ggcgaccaag agcggctcca cgtgtcaccc tgaccgccga cggtgtagac
      481 ggcgtcgagc tgcgtgagaa cgctcgtcgt cttctcgaag gtccacgacg agccgccgtc
      541 ccaggagagt ttgacgccga tcgtgccgct cgccgaactg ccggacgcct cgagtttgac
      601 gccgatgccg gtcatggcat cgttctgcgg caccgtgaag ttgaaggtac cgtacgattg
      661 ctgtacgccg cttcccgatg tcgtcacgta cgacccgtca gaggcgtacg cgttcccgcc
      721 tgctgtccat ccgttatgag gcgtcccgtt ccccgctggc gggaagtagc cggcctcgct
      781 ccctatcgtc gtgccggagt tgggatcgat cgcgccgccg cttccgccgt cggagcctcc
      841 ggacgtcgtc gtcgcgacga ccggcgtgaa gttctcaccg agcgcggtcg ctccacctcc
      901 gccgcccacc gcaccgacgc gcgcgagcgc atggaggaca cccggggcgc tccccgactt
      961 gtcgaccgca ccggagaccg cgcccacggc ggtgacatca ccggtgttca tcgcgacgta
     1021 gagccggtca gagccggaga ggttcgcgac gcttcccgtg agaatgtagt cgttcgtcgt
     1081 cgtcgcggtg aacggctcac cgaaggagat cgcgcccgcg ccgttctcca ccgagaccgt
     1141 gggtgcaccg ccgaccgccg cgtcggggcc gctgtgcgta cggtcgctgt cgttatcacg
     1201 atagaggccg acgttcgtga tgtcgttctc gccgaccccc ttcacgctcg agagctcgaa
     1261 ggcgagcgat tcgacttcga tcgactctcc gtgaggcgcg agactgaagg cgaaaagcgg
     1321 ctcgtcggta atggagccgg ttgagaactg gtcggagacc tgtccttccg agtgcgcacc
     1381 gaacgtgagc ccaccttcga tcgtgacctt cttcgcgtcg tatcccgcgg tcgtcgagcc
     1441 gctcccatgg atgagcatgc tgagcgccgt cgtagcggag ttcgccgtcc cgaaactcgt
     1501 gaacgagaga cccgagacgg taagcgtgtc gcccgcgagg aagttcacgt cgaccgatat
     1561 gacgagaacg gaatcgtcgc cctcgtacga gacgggattc gatactttgt ccgaagccgt
     1621 gccgccgaac gtcgcgctcg tatcggagga atcgaagcgc atgtcgaccg aggacgtcgc
     1681 gatagcgatg cgaatatccg tgctcgcgct aatatcgccg gccgggtcgg taatggtaat
     1741 ggtggatata cccgcggcgc tctgccccga tgtgaacgtc tggtcgtccg cggacgtgaa
     1801 gaacggcagt ccggtgtagg tcgccgtcac cgtaacgtcg aacgcgggca tgctgaacga
     1861 gcagtcgccg atgccggtac aaacgtcgtt cgtcgaactc cacccaccga acgtgtagcc
     1921 cgagggcggt gtttgtgtga tcgtgatcgc ggtgccgtag tccttctgtc ctgcggccgt
     1981 accgcctaac gtgccgccgt ttgagaccaa ggagagcgaa tgtatcgaga cggtgacgcc
     2041 gtccgaccat gaggagtacg aagagccgtt ccctgccgcg tcggtgcacg agtagcggta
     2101 gtagtacgtg ccatcgttct cgcccgtgaa cgtatggtcg ccgtccgttc ctacggaggt
     2161 cgttcccatg gcgagcgaga gcccgtcggt gtctcccaca tctacctgcg cggagcagga
     2221 cgaaagacca gcgagcgcat cggatggcgc acccgtgaag gtaagagtcg tcgtcgagac
     2281 atagtctcca tcgtcgctcg gcgtcgggac cgtcggcgcc gtcttgtcta tcttgatggc
     2341 gcccgacgat tggaccgtct ggacgttccc ggcgttgtcg gtcgatttcc agcggacgta
     2401 gttcgtcgcg gattccgtgg atacggtgac cgcggtgccc gatgttaccg ggttacaggt
     2461 gttcgtcgtg tcggtgcagt atacggtcga cgcgatgccg gagagaacat cgatggtaga
     2521 gagactgtat gtgacgtcgg aggtatacca cccgttcgtg ccggcacttc ctccggtcac
     2581 cgagcctgac gtccccggcg cgcttttatc gatcttgatc gcgcccgacg attggaccgt
     2641 ctggacgttc cccgcgttgt cggtcgactt ccagcggacg tagttcgtcg cggattccgt
     2701 ggatacggtg accgcggtgc ccgatgttac cgggttacag gtgttcgtcg tgtcggtgca
     2761 gtatacggtc g
//
LOCUS       DASTSP010000051         5147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_553778, whole genome shotgun sequence.
ACCESSION   DASTSP010000051 DASTSP010000000
VERSION     DASTSP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553778"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /gene="atpD"
                     /locus_tag="VFS75_02325"
     CDS             <1..1190
                     /gene="atpD"
                     /locus_tag="VFS75_02325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU4677531.1"
                     /translation="GLTRGLTVVDTDAPITVPVGEKALGRLMNVLGQTLDGQGQIEDA
                     PKLPIHRKPPEFTEQSTKTEVFETGIKSVDLLAPFIKGGKVGLFGGAGVGKTVLIQEL
                     IRNVATEHGGYSVFAGVGERVREGNDLYHEMKDSGVLAKTALVFGQMNEVPGARARVG
                     LTGLTMAEYFRDEGGKDVLFFMDNVFRFTQAGQEVSSLLGRVSSAVGYQPTLAEEMGQ
                     MQERITSTTKGSITSVQAVYVPADDLTDPAPATTFSHLDSTVVLSRALASLGIYPAID
                     PLGSNSSALDPHVVGKDHYEVAQGVKQVLQRYKDLQDIIAILGIEELSDDDKRLVYRA
                     RKIQRFLSQPFFVGEVFTGTPGKYVPLKETIRGFREILEGKHDDKPEQAFYMKGGIEE
                     VTG"
     gene            1196..1441
                     /locus_tag="VFS75_02330"
     CDS             1196..1441
                     /locus_tag="VFS75_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677532.1"
                     /translation="MAHTTFELTISKVDGPLYAGPAESVTLPGAAGELTVYAKHEPLI
                     ALLKKGTITVRGGEGPRTIDIERGLLEVSGTSVTVLV"
     gene            1723..2016
                     /locus_tag="VFS75_02335"
     CDS             1723..2016
                     /locus_tag="VFS75_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677533.1"
                     /translation="MFDKAKSFLMKKMLERQLKNAPAAEREMIMTLMEKNPELFQKIA
                     TEMQAEIKAGKSQTAAAMKVMPKYQAELQKALGGVPPPRSQGARFNPDGSIHT"
     gene            2083..2658
                     /locus_tag="VFS75_02340"
     CDS             2083..2658
                     /locus_tag="VFS75_02340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4677534.1"
                     /translation="MKRILLVLGHTASDSYSGRLADAYESGARSAGHEVVRVNLGDLR
                     FDPVLREGYRRVQDLEPDLKMVQEHILWADHLVFVYPNWWCTMPALLKGLFDRIWLPG
                     FAFNFDKQTKHLIKRLTGKTGRVVIAIGTQSPFMTWWRFGDFTNEISHAILGFAGVKT
                     SVTAVGPSDRVTPDVREGWVEKVRSLGARGR"
     gene            2733..3041
                     /locus_tag="VFS75_02345"
     CDS             2733..3041
                     /locus_tag="VFS75_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677535.1"
                     /translation="MELMFLLPLLALALVFASLWVVGWLNERRIDLYQYGALCAALLV
                     LAPILYVATARTLNEMSALACATVFLIGLLWCALLSKRGAWRNVRVNLAFIRYRMFGR
                     "
     gene            3115..3279
                     /locus_tag="VFS75_02350"
     CDS             3115..3279
                     /locus_tag="VFS75_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677536.1"
                     /translation="MQSRSLIWLLMGIGSAAGSYVPLLWGGSSFSFASVIFAGVGGLL
                     GIWLGYRLGS"
     gene            3329..4549
                     /locus_tag="VFS75_02355"
     CDS             3329..4549
                     /locus_tag="VFS75_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4677537.1"
                     /translation="MANNKGVLPVLFVTMLIDMVGISMLIPILPVIFTEPSSPAFMLA
                     GWSTEAQYTIAGLLTALFGFVQFIASPVLGELSDVYGRKRLITLGVAVLALSQLVFGL
                     GIAVGSLAVLFVSRAIAGVAASNFSIAQATIADVTEPHDRARNFGLIGAAVGLAFILG
                     PFLGGTIAGLSGNAALPFFVAAGLGIVNVILVSTFLKETHHVRAEKRRITPLKAVNNI
                     RAALKDHDARPVYAAGFLAMLGFAFYTSFIAILLAARFGFTETMTGTYFAIAGGWIIF
                     AQVVVVRTLAKIYSDRTRLLIALPTLAVIIVAIPLVHASHWLYVFMPFLSSAFALVTT
                     GVPSLVSKGVGPERQGAALGINGSLQALAQASAPLVAGFASGFLGLTAAFVIGAGCML
                     ASFLIIVKRVARRT"
     gene            4582..4803
                     /locus_tag="VFS75_02360"
     CDS             4582..4803
                     /locus_tag="VFS75_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677538.1"
                     /translation="MAHPFEGLFEKALMRSHGDENAVLGQAERLLDEGYPPKEICDAL
                     RSVQRSLIAAADEAIIVEALEELGICDDE"
     gene            4805..5011
                     /locus_tag="VFS75_02365"
     CDS             4805..5011
                     /locus_tag="VFS75_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677539.1"
                     /translation="MISTRIVARTFAVLIGVFFVGSLGYLAHYALTTPEPVSCPSDAF
                     ICPDGNTVGRVGPSCAFAPCPDVR"
BASE COUNT          953 a         1615 c         1549 g         1030 t
ORIGIN      
        1 cgggtctcac gcgaggactc acggtcgtcg ataccgacgc ccccatcacc gtgccggtcg
       61 gcgagaaggc gctcggccgg ctcatgaacg tgctcggaca aacgctcgac ggacagggtc
      121 agatcgagga cgctccgaaa ctccccattc accggaaacc gccggagttc accgagcagt
      181 cgacgaagac cgaggtcttc gagaccggca tcaagtcggt cgacctcctc gcaccgttca
      241 tcaagggcgg taaggtcggc ctcttcggcg gcgcgggcgt cgggaagacc gtcctcatcc
      301 aggagctcat ccgtaacgtc gcgaccgagc atggcgggta ttccgtcttc gccggtgtcg
      361 gtgagcgcgt gcgcgagggt aacgatctct accacgagat gaaggattcg ggcgttctcg
      421 cgaaaacggc gctcgtcttc ggacagatga acgaggtgcc gggcgcgcgt gcgcgcgtcg
      481 gactcactgg gctcacgatg gcggagtact tccgtgacga gggaggaaag gacgtcctct
      541 tcttcatgga caacgtcttc cgcttcacgc aggccggtca ggaggtatcg tcgctcctcg
      601 gccgcgtgtc gtcggcggtg gggtaccagc cgaccctggc tgaggagatg ggacagatgc
      661 aggagcgcat cacctcgacg acgaagggct ccatcacgtc ggttcaggcc gtatacgtgc
      721 cggcagacga cctcaccgat cccgcaccgg cgacgacctt ctcgcacctc gactcgacgg
      781 tcgtcctctc tcgcgccctc gcgtcgctcg gcatctatcc ggctatcgac ccgctcggta
      841 gtaactcatc ggcgctcgac ccccatgtcg tcggcaagga ccactacgag gtcgcgcaag
      901 gcgtgaagca ggtcctccag cggtacaagg acctccagga catcatcgcc attctcggta
      961 tcgaagagct ctccgacgac gacaagcgac tcgtgtaccg tgcgcgaaag atccagcgat
     1021 tccttagcca acctttcttc gtcggcgagg tcttcacggg tacccctggc aagtatgtac
     1081 ccctgaagga gaccatccgc ggctttaggg agatactcga agggaaacac gacgataagc
     1141 cggagcaggc cttctacatg aagggcggca tcgaggaggt aaccggctag cgcatatggc
     1201 acacactacg ttcgaactca ccatatcgaa ggtcgatggc ccgctctatg cgggtccggc
     1261 cgagtccgtg acgcttcccg gcgccgcggg agagcttacc gtgtacgcga agcacgagcc
     1321 gctgatcgcg ctcctcaaga aggggacaat cacggtccgt ggtggagagg gcccgcgcac
     1381 catcgacatc gaacgcggcc tcctcgaggt gagcggcacg agcgtcaccg ttctcgtata
     1441 aaaaaaccgc gcacgagagt gcgcggcgaa gactggaggt gccgaatcgc tcggcacgga
     1501 gagtgtcgca catggcggac gtttccgcaa gcaaaaaaag agggttacga gcttgcgccc
     1561 gtaaccccgt ttgtggcctc tagtacatct agatcaaagg agtgatccgg acgaggtacg
     1621 aggagagggt cctcgcgccc gaatcaacac ctactatgac atgaagttgg aacgcacgca
     1681 agcccactcg atttaccttt aaaatgtgct atacatacac ctatgttcga taaagcgaag
     1741 agttttttga tgaagaagat gcttgagcgt cagctcaaga acgcgccagc cgccgagcgc
     1801 gagatgatca tgaccctcat ggagaagaat cccgagctct tccaaaagat cgcgacggag
     1861 atgcaggcgg agatcaaggc ggggaagtcg cagaccgcgg ccgccatgaa ggtgatgccg
     1921 aagtatcagg cggaactcca gaaggcgctc ggtggcgttc ctccgccgcg ctcgcaaggg
     1981 gcacgattca acccggacgg ctcgatacac acgtagacgt ttgttcacag aaccccggac
     2041 gctcaagcgt ccggggttct ctcatatgct acgatggtat ccatgaaacg gatcttactc
     2101 gttctcggcc ataccgcgtc cgactcgtac tcgggacggc tcgcggacgc gtacgagtca
     2161 ggtgcccgaa gcgccggtca tgaggtcgtt cgcgtgaacc tcggagacct acggttcgac
     2221 ccggtcctac gtgagggcta ccgccgcgtc caagacctcg agcctgacct caagatggtc
     2281 caggaacaca tcctctgggc cgaccacctc gtattcgtat accctaattg gtggtgcacg
     2341 atgcccgccc tcctcaaggg tctcttcgac cgcatctggc ttcccggctt cgccttcaat
     2401 ttcgacaagc agacgaagca cctcatcaag cgccttacgg gaaagacggg ccgcgtcgtc
     2461 atcgcgatcg gcacccagag tccgttcatg acgtggtggc ggttcggaga ttttacgaac
     2521 gagatatcgc acgcgatact cgggttcgcg ggtgtgaaga cgtccgtgac cgcggtaggg
     2581 ccttccgacc gcgtgacgcc agacgttcgc gaaggctggg tagagaaggt gcgctcgctc
     2641 ggcgcgaggg ggaggtagtt gacaccgacc ctttacaaac aggcacccag gcggcactct
     2701 agtgtgggcc attcaccaaa aaggtgcgta cgatggaact catgttctta ctgccgcttc
     2761 tcgcgctcgc cttggtattc gcctcgctct gggtcgtcgg ctggcttaac gagcgacgga
     2821 tcgacctcta tcagtacggc gcgctctgtg ccgcgctcct cgtgctcgct cccatcctct
     2881 acgtcgcgac cgcgcggacg ctgaacgaga tgagcgcgct cgcgtgcgcg acggtctttc
     2941 tcatcggcct tctctggtgt gcgctcctta gcaagcgggg tgcctggagg aacgtgcggg
     3001 tgaacctcgc cttcattcgc taccgaatgt tcgggagatg agtgcgacct ttcggccgga
     3061 agttattccg gccttttttg tgcgcggcaa agagaagact gctatgattt cggtatgcaa
     3121 tcgcgttccc tcatatggct cttaatgggt atcggctccg ccgcggggag ttacgtgccg
     3181 ctcctctggg gcgggagttc cttctcgttc gcttcggtca ttttcgcggg cgttgggggc
     3241 ctcctcggta tttggctcgg ctatcgtctc ggctcgtagg atgaataact tcatcgcgac
     3301 ctaaccgctg cggtatatca tatgcaccat ggcgaacaat aaaggtgtgc ttcccgtcct
     3361 cttcgtgacc atgctcatcg acatggtcgg gataagcatg ctcatcccca tcctgccggt
     3421 catctttacg gagccgtctt cgcccgcgtt catgctcgcg ggatggagca ccgaggcgca
     3481 gtacacgatc gcgggtctcc tcaccgcgct cttcggcttc gtccagttca tcgcgtctcc
     3541 ggtacttggc gaactctccg acgtctacgg gcggaagcga ctcatcacgc tcggcgtcgc
     3601 cgttctcgcg ctctcccagc tcgtcttcgg tctcggtatc gcggtcggtt cgctcgccgt
     3661 tctcttcgtg agtcgcgcca tcgcgggtgt cgccgcgtcg aacttcagca tcgcgcaggc
     3721 gacaatcgca gacgtcacgg aaccgcacga tcgggcccgt aacttcggac tcataggcgc
     3781 cgccgtcggc ctcgcgttca tcctcggtcc gttcctcggc ggtacgatag cgggtctaag
     3841 cgggaacgcg gcgctcccgt tcttcgtcgc ggcgggactt ggtatcgtga acgtcatcct
     3901 cgtctcgacc ttcctcaagg agacgcatca cgtccgcgca gagaagcggc gcatcacgcc
     3961 gcttaaggcc gtcaacaata ttcgtgcggc gctcaaggac cacgacgcgc ggccggtcta
     4021 cgccgccggt tttctcgcga tgctcggctt cgcgttctat acgtcgttca tcgcgatact
     4081 cctcgcggct cgattcggat tcaccgagac gatgaccggc acgtacttcg cgattgcggg
     4141 tgggtggatc atcttcgcgc aggtagtagt cgttcgcact ctcgcgaaga tctacagcga
     4201 tagaacgcga ctcctcatcg cgctcccgac cctcgccgtc atcatcgtgg cgataccgct
     4261 cgtccacgcg tcccattggc tctacgtgtt catgccgttt ctctcgtcgg ccttcgcact
     4321 cgtgacgacc ggcgtgccgt ctctcgtgag taaaggcgtc ggacccgaac gccagggagc
     4381 agccctcggc atcaacggct cgctccaggc cctcgcgcag gcgtcggcac cgctcgtcgc
     4441 cggcttcgca tccgggttcc tcggtctcac cgcggcgttc gttatcggtg cgggttgcat
     4501 gctcgcctcg tttctcatca tcgtgaagcg cgtcgcgcgg cgtacttgac gaggcgggcg
     4561 gttctcgccg aaaatcatcg tatggcacac ccgttcgagg gactattcga gaaggcgctc
     4621 atgcgcagtc acggcgacga gaacgccgtt ctcgggcaag ccgagcgcct tctcgacgag
     4681 gggtatccac cgaaggagat ttgcgacgcg cttcgaagcg tgcagcgctc gctcatcgcc
     4741 gccgctgacg aggcaatcat cgtagaggcg ctcgaggagc tcggcatatg cgatgatgag
     4801 taacatgata tccacgcgaa tcgtcgcacg taccttcgcc gtcctcattg gtgtcttctt
     4861 cgtcggctcg cttggatatc tcgcgcacta cgccctcact acgccggaac ccgtctcgtg
     4921 cccgagcgac gccttcattt gcccggacgg caatacggtc ggccgcgtcg ggccgtcatg
     4981 cgcgttcgct ccctgtcccg atgtgcgctg accggttttg acagtaggcg gaaggggacg
     5041 taggcttttt cgagatgcac ctcgcaaaca acaagggaga ccgccatggt ggaggttgac
     5101 gtgatgatcg aaggcgtgag cgagtacacg cgcgtcatcg ccgcatt
//
LOCUS       DASTSP010000052         7198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1448057, whole genome shotgun sequence.
ACCESSION   DASTSP010000052 DASTSP010000000
VERSION     DASTSP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448057"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1415
                     /locus_tag="VFS75_02370"
     CDS             354..1415
                     /locus_tag="VFS75_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677540.1"
                     /translation="MRKDMEHDSNSSYASTRPRGWLYIALFALFAAPHTSHAALLYLD
                     PAATELHRGDTVTMALRLDVDEGECVNTIRGVVHYDPSIRAVDVSRGDSIFSIWVEDP
                     LIDEAAHTITFAGGIPGGYCGRIPGDPSLTNVILRIVFRAPGFTVGAGSEPEAKVSID
                     EGSEAYLNDGVGTAAPLRVADATVSLLPTAGDTLSDDWRTVVQNDKDAPADFDITLAT
                     STTAFSGKYFITFNSADKQSGIDHYEVMEEPFTEWSAFKWGAADAPWTKAESPYVLKD
                     QSLNSTIRVKAIDKAGNVRIETLVPDTALRSISRDRLLTILVFTGLGVVLAAVVFYAL
                     WRRRNRILAEEAQKETEIL"
     gene            1420..3144
                     /locus_tag="VFS75_02375"
     CDS             1420..3144
                     /locus_tag="VFS75_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEU4677541.1"
                     /translation="MKSLRILSTFLTATALFLVAGHAMAATLRLNPGTGVYTVGTPFT
                     VSILIGTDGKPVNAADGSLSFNPRELQVVGVSRAASIFSLWTAEPSFSNAAGTISFSG
                     GSPSGYTGSAGMVATVTFKPLGAGTPKVNFQSGSILAADGLGTNVLTGMSGGTYTVSA
                     PTENPEPEYITPPNTPKAPVVTSTTHPDQSGWYKSTTAKLAWDLPSGVTAVRTLLDSS
                     PSTIPTKTYDDLITEKTIDDLDEGVSYFHIQFKNAEGWGKITHYRLGVDTEAPKDFTI
                     AEADASDSPERALSFDYDDVSPVVRYKIQIDGKDPIDYADEKGTKRYTVPPLSPGYHT
                     FIVEAFDGAGNSTIATYSMTIEAFEKPILTDYPSRIDTDVIPAIKGTTRPHATVAVSI
                     VPASAGDDTPSPTDADFTVTANDEGEFVYIPDHSFTEGVYLLSAVAKDEFGRMSERSD
                     VVRLLVEKPGYIRLGSMVVNALSVIVPLLALLILLVFGTWYLWHRLANWAKRVRKETK
                     EAEASLAAEFDRIVRNLDDKVTSLRASRKGKLTKAEAELIDQIEGDLKTARSRIGKEV
                     GDIEDIVS"
     gene            3141..3812
                     /locus_tag="VFS75_02380"
     CDS             3141..3812
                     /locus_tag="VFS75_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677542.1"
                     /translation="MTTPTVKYLETHLMVALALLTVALGIAGGAYVAHLRIERAESLV
                     REELTRIGETMYAIAETTDRNGIDESAAGIITNCERRGEFDALLTNLGTLGKKDLITV
                     QSMFDACGRVDAKQKAFMVSRLTREHEEYEDLLHLLAKLSGQGVDAYHDDAWTRIVET
                     ERLRSDLLADLSTIQANIISNLILGKGTGSAEVREQVAEAQNIGQRLIVYDRTIDELR
                     ASVKP"
     gene            3809..4552
                     /locus_tag="VFS75_02385"
     CDS             3809..4552
                     /locus_tag="VFS75_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677543.1"
                     /translation="MKMFRVLLKMLLPRYADGRFATVRYAFPVVLASAIFASLAAVMT
                     ESTSFITIETDATTVTRDQQFTIDVGITARVPINAVDLVISYPSDSLVVDSIDTGMSV
                     ITLWAEEPYAKDGSIFLRGGTFRKGFIGEHDIARIKAHATKAGEARILLKDLQFVAAD
                     GKGTYLPVDPSLTNAVNVVVEGTDGVASGKVGISLVTDLDGDGDVDLKDVSAFMTAWF
                     SGTRVYDFNHDGRMTFSDFSILLSDSFFR"
     gene            4786..6504
                     /locus_tag="VFS75_02390"
     CDS             4786..6504
                     /locus_tag="VFS75_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677544.1"
                     /translation="MLLPLQPAFAAEEGGALPDQNPPTPVEPSTEPSPTAAPPVLLEP
                     VAEDEPATPLAEDEPVVADTVDSTEPTVSSGDTDIESPLTTEPVVTTDTQAVTAVEPE
                     NGGASSTASTASSTSTDEGGGAESNMSDDNAPEATTTASLPETDAQAPAEATEGTTTA
                     PVLDTEGVSTTTEDTRDVSLDDATVEVAPADDLPAAPISDETARKGGETVSVNTVTND
                     ANRFTFDKDACVRVGDGSFYCSSEPSVPAVTGTDRVFAAVDDDGDREIYVEKSGTVRA
                     ITDNSMDDDAPYFDEASNSIVWHRLVDGRYQIVEYDVATNEEHTLTHDRYNNMEPSRY
                     GALTVWQAWVGNDWEIMLDDSGTTTMITDNTYHDISPRVSGTYVVWQAFEDGAWRVKV
                     YDALTKKTETIGDAGGASLENPRFVLVYDSKQENGDVETRGYDLHTKKNVPLESTPAP
                     VPDRLPNPDETGEHRALVQPPVQLKTKDGSGADVPIVGGSDPDDDIASSTDRTSDELA
                     GPTLDLTEGTTTAATSPEAEDLVVTPLPDHATSTATDHIDDVIVVPFSAGVLDTDPQT
                     GVASST"
     gene            6524..>7198
                     /locus_tag="VFS75_02395"
     CDS             6524..>7198
                     /locus_tag="VFS75_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677545.1"
                     /translation="MGGNLPSMLFTRRRSIAVFVGFVLLSGAVALSARFAPIAEAAGN
                     DFNIQRGTFTMTGTTKTLTAGTDYVAPTASTSAFIRITNIGGTGAGDASASVPQAARD
                     VTAYVQDPSDIMTSVTFARDASATGTTRISWEIVEFTGESGSDNEMIVRSQSTVTYGG
                     SSLTATGTAATGVADDADIVVFITGEENPNAGSTQYNTGESTSAWDGGTNQPVFTRGE
                     AGGDAAI"
BASE COUNT         1483 a         2296 c         2188 g         1231 t
ORIGIN      
        1 cattacaaga agcgggtgac gattattcta cgacacttcg ctacattgta acgcccacgt
       61 tctagcaacg gcacagagta tgaaaagccc tatctcccac cacgagaagg gtttttcatt
      121 tgctcgagcg cgaatgaaaa acccttctcg tggcgggcaa gcccaccgtc ctacggacgc
      181 ggtatacctt tcgcagcaag tgctcaaggt catgcggaat gtccccgaca tttctcacct
      241 gaacagcccc tgtaaggggt ttcatgtccg ggggacatga aaaagagatt gtcggcgcgg
      301 tcggcctcac atgggtaaaa gcgcggagga tgaacgttcc cataccgtat actatgcgta
      361 aggatatgga gcacgactcg aattcgtcgt acgcgagcac gcgaccgcgc gggtggctct
      421 acatcgcgct cttcgctctc ttcgcggcac cgcacacgtc acacgcggcg ttactgtacc
      481 tcgatccggc ggcgaccgag ctccatcgcg gtgatacggt caccatggcg ctccgcctcg
      541 atgtcgacga aggggagtgc gtgaacacga tacgcggcgt cgtccactat gacccgtcga
      601 tacgcgccgt cgacgtctcg cgcggcgatt cgatattctc gatctgggtc gaggaccctc
      661 tcatagacga agccgctcac accatcacgt tcgcgggcgg cataccgggc ggctactgcg
      721 gccgcatacc gggcgaccca tccctcacga acgtcatcct tcgcatcgtc tttcgcgcac
      781 cggggttcac cgtcggcgcg ggttcggaac cggaggcgaa ggtatcgata gacgagggct
      841 ccgaggcgta cctcaacgac ggtgtcggca ccgcggcacc gctccgcgta gcggacgcga
      901 cggtatcgct ccttccgacg gcgggggata cgctctcaga cgactggcgc acggtcgttc
      961 agaacgacaa agacgctccc gccgatttcg atatcacgct cgcgacgagc acgaccgcct
     1021 tcagcggcaa gtacttcatc acgttcaatt cggctgataa gcaatccggt atcgaccact
     1081 acgaggtcat ggaggagccg ttcacggaat ggagcgcgtt caagtgggga gccgccgatg
     1141 cgccgtggac gaaggcggag agtccgtacg ttctcaagga ccagtcgctc aatagtacga
     1201 tacgcgtgaa ggcgatagac aaggccggta acgttcgcat cgagaccttg gttcccgaca
     1261 cggcgctccg gagcatctcc cgcgatagac tccttaccat cctcgtattc accgggctcg
     1321 gggtcgttct cgccgccgtc gtcttctatg ccctctggcg acgcaggaat cgcatcctcg
     1381 ccgaagaggc acaaaaggag accgagatcc tataacgtca tgaagtccct tcgcatactc
     1441 tcgacgttcc ttaccgccac cgcgctcttt ctcgttgccg gacacgcgat ggcggcgacg
     1501 ctccgactca atccggggac gggggtctac accgtcggga cgccgttcac cgtgagtatc
     1561 ctcatcggta cggacgggaa gccggtcaat gcggcggacg gcagtctttc gttcaacccg
     1621 cgcgaacttc aggtcgtcgg cgtctcgcgc gccgcgtcga tcttcagtct ctggaccgcc
     1681 gaaccgtcgt tctcgaacgc ggcgggtacg atctccttca gcggcgggtc gccgtcgggg
     1741 tacaccggta gcgccggcat ggtcgcgacc gtcaccttca agccgctcgg cgcaggcacg
     1801 ccgaaggtca acttccagag cggttcgata ctcgcggctg acggactcgg caccaacgtc
     1861 ctcacgggca tgagcggcgg cacgtacacc gtctcggcgc ctacggagaa cccggaaccc
     1921 gagtacatta cgccgcccaa taccccgaag gcgccggtcg tcacgtcgac gacgcatccg
     1981 gaccagagcg gttggtacaa gtcgacgacc gcgaagctcg cgtgggacct cccctcgggc
     2041 gtcaccgccg ttcggacgct cctcgactcc tcgcccagca cgataccgac gaagacgtac
     2101 gacgatctca tcacggagaa gaccatcgat gacctcgacg agggagtttc gtatttccat
     2161 attcagttca agaacgccga gggatggggc aagatcacgc actatcgact cggggtcgat
     2221 accgaggcgc cgaaggactt cacgatagcc gaagcggacg cgagcgacag tcccgagcgc
     2281 gcgctctcct tcgactatga cgacgtgtcg ccggtcgtac gctacaagat acagatagac
     2341 gggaaagacc cgatcgacta cgccgacgaa aagggtacga agcgctacac cgtgccgcca
     2401 ctttcgccgg ggtaccatac gttcatcgta gaagcgttcg acggcgcggg gaatagcacc
     2461 atcgcgacgt actcgatgac catcgaggcc ttcgagaagc ctattctcac cgattacccg
     2521 agccgcatag acaccgacgt cataccggcg atcaagggta cgacgcggcc gcatgcgacc
     2581 gtcgccgtct ccatcgttcc ggcgagcgcg ggcgacgata cgccatctcc gaccgacgcg
     2641 gacttcaccg tgacggcgaa cgacgagggt gagttcgtct acatacccga ccactcgttc
     2701 accgagggtg tgtacctcct ctccgcggtc gcgaaggatg agttcggtcg catgagcgag
     2761 cgctccgacg tcgtccgcct cctcgtcgag aagcccggtt acatccgact cggctcgatg
     2821 gtcgtgaacg cgctctccgt catcgtgccc ctcctcgcgc tcctcatact cctcgtgttc
     2881 gggacatggt acctctggca ccgcctcgcg aactgggcga agcgggttcg gaaggagacg
     2941 aaggaggcgg aagcgagcct cgccgcggag ttcgaccgca tcgtgagaaa cctcgacgat
     3001 aaggtcacat cgctccgcgc gtcccgaaag gggaagctca cgaaggccga ggcggagctc
     3061 atcgaccaga tcgagggaga cctcaagacc gcgcgtagcc gcatcggcaa ggaggtagga
     3121 gacatcgaag acatcgtatc atgacgacac caaccgttaa gtatctcgaa acgcatctca
     3181 tggtcgccct cgcgctcctc acggtcgcct tgggcatcgc gggtggcgcc tacgtcgcgc
     3241 atctgcggat cgagcgcgcc gagtcgctcg ttcgcgagga actcacgcga atcggtgaga
     3301 ccatgtacgc catcgcggaa acgactgacc gtaacggcat cgacgagtcg gccgcgggca
     3361 tcatcacgaa ctgcgaacgc cgcggcgagt tcgacgcgct ccttacgaac ctcggcaccc
     3421 tcgggaagaa ggacctcatc acggtgcaat ccatgttcga cgcgtgcggt cgcgtcgacg
     3481 cgaagcagaa ggcgttcatg gtgtcgcgtc tcacgcgcga gcacgaagag tacgaagacc
     3541 tcctccacct cctcgcgaaa ctctccggtc agggggtaga cgcgtaccat gacgacgcgt
     3601 ggacgcggat agtcgagacc gagcgtcttc ggagcgacct cctcgcggat ctaagcacga
     3661 ttcaggcgaa catcatatcg aatctcattc tcggcaaggg cacggggagc gcggaggtgc
     3721 gcgagcaggt cgccgaggcg cagaacatcg gtcagcgcct catcgtgtac gaccgcacca
     3781 tcgacgaact ccgtgcgtcc gtaaaaccat gaagatgttc agagtcctct tgaagatgct
     3841 ccttccgcgg tacgcggacg ggcggttcgc gaccgtacgg tacgcctttc ccgtcgttct
     3901 cgcgagcgct atcttcgcga gcctcgccgc ggtcatgacc gaatcgacgt cgttcatcac
     3961 tatcgagacc gacgcgacga ccgtcacgcg cgaccagcag ttcacgatag acgtcggtat
     4021 caccgctcgc gtcccgataa acgcggtcga cctcgtcatc tcatatccgt ccgattcgct
     4081 cgtcgtcgat agtatcgata ccggcatgtc cgtcatcacg ctctgggcgg aggaacccta
     4141 cgcgaaggac ggtagcatct tcctgcgcgg ggggacgttc agaaagggat tcatcggcga
     4201 gcacgacatc gcgcgcatca aagcgcacgc gacgaaggcg ggggaggcac gcatcctcct
     4261 taaagacctc caattcgtcg cggccgacgg aaaggggacg tacctgccgg tcgacccgtc
     4321 cctcacgaac gcggtcaacg tcgtcgtcga gggcacggac ggcgtcgcgt cgggcaaggt
     4381 cggcatttcg ctcgttaccg acctcgacgg agacggcgac gtcgatctca aggacgtgag
     4441 cgccttcatg accgcatggt tctccggaac gcgtgtctac gatttcaatc atgacggccg
     4501 catgacgttt agcgactttt cgatactcct ctcggattcg ttcttccggt agttatccac
     4561 agcggtacgg ccgtcggtgc ataaagtccg ccgcccgtgt tgataaggat gtggataggg
     4621 tctggaagcc ctgtagaata tctgtataca ggctcatcgg cacttttacg ggcctgttcg
     4681 tagagcatgc gaaagcatca tgtaacgcga cgagcgcaca aacggctccg aggttttgag
     4741 tatctggcat actgcgcttt ttcttcgaca cttcttttcg tacttgtgct ccttccgctc
     4801 caaccggcgt tcgccgcgga ggaaggggga gcactaccgg atcagaaccc tccgacgccc
     4861 gtagaaccgt cgaccgagcc atcgcccacg gccgcgccgc ccgtgctctt agaacccgtc
     4921 gccgaagacg aacctgccac gcctctggcc gaggatgagc cagtggtagc cgacaccgtc
     4981 gactcgaccg aaccgaccgt ttcctcgggc gacactgaca tcgaatcgcc tcttaccacc
     5041 gagcccgtcg tgacgacgga tacgcaagcg gtgaccgcgg tcgaaccgga gaacggcggc
     5101 gcgtcgagta cggcatcgac cgcgagcagt acctcgacgg acgagggcgg cggcgccgag
     5161 tcgaacatga gcgacgataa cgctccggaa gcgacgacga ccgcgtcgct cccggagacg
     5221 gacgcgcaag cgcccgccga ggcgacggag ggaacgacga ccgcgcccgt acttgatacc
     5281 gagggagtct cgacgacgac ggaggacaca cgagacgtct cgctcgacga tgcaacggtc
     5341 gaggttgcgc ccgcggacga tctacccgcc gcgccgattt ctgacgaaac tgcgagaaag
     5401 ggcggcgaga ccgtttcggt gaacacggta acgaacgatg ccaatcgctt cacgttcgat
     5461 aaggatgcct gcgtgcgggt cggcgacggc tcgttctact gctcgagcga gccttcggtg
     5521 cccgcggtga cgggaactga ccgcgttttc gccgccgtcg acgatgacgg cgaccgcgag
     5581 atctacgtcg agaagtccgg tacggtccgc gcgataacgg acaactcaat ggacgatgac
     5641 gccccctact tcgatgaggc gtcgaacagt atcgtctggc atcgcctcgt cgatgggcgg
     5701 taccagatcg tcgagtacga cgtggcgacg aacgaggagc acactctcac gcacgaccgc
     5761 tacaacaata tggaaccgtc gcgatacggc gcgctcacgg tctggcaggc atgggtgggg
     5821 aacgactggg agatcatgct cgacgatagc ggcacgacga cgatgatcac ggacaacacg
     5881 taccacgaca tctcgccacg ggtgagcggc acgtacgtcg tctggcaggc tttcgaggat
     5941 ggcgcatggc gcgtcaaagt gtacgatgcg ctcacgaaga agacggagac gatcggcgac
     6001 gccggcggtg cgtcgctcga gaatcctcga ttcgtcctcg tgtacgactc gaagcaggag
     6061 aacggcgacg tcgaaacgcg cggctatgac ctccatacga agaagaacgt gcccctcgag
     6121 tcgactcccg cgccggtacc ggaccgactt ccgaatcccg atgagacggg agagcaccgc
     6181 gcgctcgttc agccgccggt acagctgaag acgaaggacg gaagcggcgc cgacgtgcct
     6241 atcgtcggcg gcagcgatcc ggacgatgac atcgcatcga gcacggatcg aacgagcgac
     6301 gagctcgctg gccccacgct cgacctcacc gagggcacga ccaccgcggc aacgtcgccg
     6361 gaagccgagg acctcgtcgt gacgccgctt cccgaccacg cgacgagcac cgcgaccgac
     6421 cacatcgatg acgtcatcgt cgtacccttc tcggcgggag ttctcgatac cgacccacag
     6481 accggggtcg cttcgtcaac ctaactggca cataatagag agaatgggag ggaatctgcc
     6541 ttcgatgctt tttacgcgaa gacgttcgat cgccgtgttc gtcggcttcg tgctcttaag
     6601 cggcgcggtc gctctctccg cgcggttcgc gcccatagcg gaggccgccg gcaatgattt
     6661 caatatacag cgagggacgt tcacgatgac gggaacgacg aagacgctca ccgctgggac
     6721 ggattacgtc gcgccgaccg cttcgacatc cgcgttcata cgtatcacga acataggcgg
     6781 aaccggcgcc ggcgatgcgt cagcgtctgt tccacaggcg gcgcgcgacg ttaccgcata
     6841 cgttcaagac ccatcggaca tcatgacctc ggtgactttc gcgcgcgacg catcggcgac
     6901 ggggacgacg cgcatctcat gggagatcgt cgagttcacc ggagagtcag gttcggacaa
     6961 cgagatgatc gtgcgcagcc agtccaccgt tacgtacggt ggcagttcgc tgaccgcgac
     7021 cggtacggcc gcgaccggcg tcgcggacga cgccgacatc gtcgtcttca taacgggtga
     7081 ggagaacccg aacgcgggct cgacgcagta caacacggga gagtcgacct ccgcatggga
     7141 cggtggcacg aaccagccgg tcttcactcg aggagaggcg ggcggggacg cggccatc
//
LOCUS       DASTSP010000053         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1449504, whole genome shotgun sequence.
ACCESSION   DASTSP010000053 DASTSP010000000
VERSION     DASTSP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449504"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..2066
                     /locus_tag="VFS75_02400"
     CDS             189..2066
                     /locus_tag="VFS75_02400"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU4677546.1"
                     /translation="MTGNQVRERFLAFMKERGHVIIPSSQLVPENDPTTLFTGSGMQP
                     LVPYLMGESHPSGTRLADSQKCFRAEDIDEVGDNRHTTFFEMLGNWSLGDYFKKEQIP
                     WFYEFLVDSIGLDPARLYVTVFMGDESAGVPRDTESAELWQGLFGARGVSSGIAEMGS
                     EAAGGERGMKEGERIFYYDASKNWWSRAGRPEKMPIGEIGGPDTEVFYDFGTPHDPKF
                     GRACHPNCDCGRFLEIGNSVFMQYVKNADGSFAMLPKQNVDFGGGLERITAASLLDGD
                     VFKVDLLRNVVLEIERLSGKRYEDDTTSFRIITDHIRGAVFMIGDGVYPGNVEQGYFT
                     RRLIRRAVRYADIVGIPKGELKLLAKSVIDTYKTHYENLGAHEADIVGTIYDEETQFR
                     KTLENGLREFNKVSDKGERRITGEEAFTLFTTYGFPIELTSEIAAERGMTVDLDGFKT
                     HMAEHRERSRAGAAEKFKGGLADHSEKVIMYHTATHLLLAALRKELGEHVHQAGSNIT
                     GERLRFDFTHPDKLTDETLRKLEAYVNEAIRKNARVSIETLPKAVAEKDPTIEGSFWD
                     RYPDTVKVYTIKDDEGTVYSRELCGGPHVDATGTISGTFKIQKEESSSRGVRRIKATL
                     E"
     gene            2121..>3019
                     /locus_tag="VFS75_02405"
     CDS             2121..>3019
                     /locus_tag="VFS75_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677547.1"
                     /translation="MLFDRSGTKTTYGALIDVASGTIGIAIVASDAVKKLPEIIYENR
                     IDMRVGKRAAAEIRRVREMLLSASLSFSEGFASLRAIDPRARVSSLSVTVAAPWSYTV
                     TNAVEYKDDAPFKITPTMLRDLVDSAEATILEGMRKDPDAAAENLEIIERVTTNVTVN
                     DYPVPDPLKVEGTSLALSHVVGLVPRDILTSVHEIQDKLFQGTTLRVHTSMCTLFAVA
                     EELYPRLSSFCLVNVSGEATEFGIATEGTLTDSIFIPKGTNGFMKKVMEDTGRPLADV
                     ESALRAFGAKDAAMASDLEAHLS"
BASE COUNT          641 a          920 c          904 g          554 t
ORIGIN      
        1 taggaagtcc tcatggtttt cgcgcgggtg cacgggcaca ttcggaaggg tctcactgct
       61 tgcctccttg ggtcgtttac gcctgagaca tctcaggttt cgctatcatg ccatatgttc
      121 caattttgtc cagtatcccg ccatctaccg ctagaatgag cggaaaaagt gtagtatagt
      181 cattttctat gaccggcaat caggtacgag agcgattcct cgcgttcatg aaagagcgcg
      241 gacacgtcat catcccgtcc tcacaactcg ttcccgagaa cgaccccacg accctcttca
      301 cgggaagcgg tatgcagccc ctcgtcccgt acctcatggg cgagagccat ccatcaggca
      361 cgcgtcttgc ggactcgcag aagtgtttcc gcgcggagga tatcgacgag gtcggcgaca
      421 accgccacac gaccttcttc gagatgctcg gtaactggtc actcggcgac tatttcaaga
      481 aggagcagat accctggttc tacgagttcc tcgtcgactc gatcggactc gaccccgcgc
      541 ggctctacgt gaccgtgttc atgggcgatg aaagcgctgg cgtgccgcgc gacaccgagt
      601 cggcggaact ctggcaagga ctcttcggag cgcgtggcgt ctcaagcggt atcgcagaga
      661 tgggctccga ggccgcgggc ggcgaacgcg gtatgaagga aggggagcgc atcttctact
      721 acgatgcctc gaagaactgg tggtctcgcg ccggaagacc ggagaagatg ccgatcggcg
      781 agataggcgg tcccgacacc gaggtcttct acgacttcgg tacgccacac gacccaaagt
      841 tcggtagagc gtgtcacccg aactgcgatt gcggacggtt cctcgaaata gggaactccg
      901 tcttcatgca gtacgtgaag aacgctgacg gttcgttcgc gatgttgccg aagcagaacg
      961 tcgatttcgg cggcggactc gagcgcatca ccgcggcgtc gctcctcgac ggtgacgtct
     1021 tcaaggtcga cctcctccga aacgtcgtcc tcgagatcga gcgactctcc ggaaagcgtt
     1081 acgaggacga taccacgtcc ttccgcatca ttaccgacca catccgcggc gcggtcttca
     1141 tgatcggcga cggcgtttac cccggaaacg tcgagcaggg atacttcacg cggcggctca
     1201 tccggcgcgc ggtccgttac gcggacatcg tcggtatacc gaagggggaa ctcaagctcc
     1261 tcgcgaagag cgtcatcgac acgtacaaga cgcactacga gaacctcggt gcgcatgagg
     1321 cggacatcgt cggcaccata tacgacgaag agacacagtt taggaagacg ttggagaacg
     1381 gactacgaga gttcaataag gtctccgaca agggggagag gcgaataacc ggcgaagagg
     1441 cgttcaccct gttcacgacg tacggattcc cgatcgagct cacgagcgag attgccgccg
     1501 aacgcggcat gaccgtcgac ctcgatggtt tcaaaacgca tatggcggaa catcgcgaac
     1561 gttctcgtgc gggagcggcg gagaagttca agggtggtct cgcggaccac tcggagaagg
     1621 tcatcatgta ccacaccgcg acgcaccttc tcctcgccgc gcttcgcaaa gaactcggcg
     1681 aacatgtcca tcaggccggt tcgaacatca ccggcgagcg gctccggttc gatttcacgc
     1741 atcccgacaa gctcacggac gagaccttgc gaaagctcga ggcgtacgtg aacgaggcga
     1801 tacggaagaa cgcgcgcgtc tccatcgaga cgctcccgaa ggcggtcgcc gagaaggacc
     1861 cgacgatcga gggaagcttc tgggaccgat atcccgacac cgtgaaggtg tacaccatca
     1921 aggacgacga ggggaccgtg tactctcgcg agctttgcgg cgggccgcac gttgacgcga
     1981 ccggtaccat ctccggcacg ttcaagatac agaaggaaga atcatcctcg cgcggcgtgc
     2041 gacgcatcaa agcgacgctc gagtagacgc aatgtagatt attcgcgagg cgctccgcta
     2101 cgttgcggta tactggttcc atgctctttg atcgaagtgg cacgaagacg acgtacggcg
     2161 ccttgatcga cgtcgcctcc ggcacgatcg gtatcgccat cgtggcctcg gatgccgtga
     2221 agaagctccc cgagataatc tacgagaacc gcatcgacat gcgagtcggc aagcgcgccg
     2281 ccgcggagat tcgtcgcgta cgtgagatgc tcctctccgc ctcgctctcg ttctcggagg
     2341 gcttcgcgtc gcttcgcgct atcgacccgc gagcgcgagt ctcctcgctc tcggtcaccg
     2401 tcgccgcacc ctggtcgtac acggtgacga acgcggtcga atacaaagac gacgcgccgt
     2461 ttaagatcac gccgaccatg cttcgcgacc tcgtcgacag cgccgaggcg accatcctcg
     2521 agggaatgcg gaaggacccc gacgccgcgg cggagaacct cgagataatc gaacgggtga
     2581 cgacgaatgt caccgtgaac gactacccgg taccagaccc actgaaggtc gagggcacgt
     2641 cgctcgcgct ttcccatgtc gtcggtctcg taccgcgcga catcctcacg tcggtccatg
     2701 agatacagga taagctcttt cagggtacga cgctccgcgt tcatacgtcc atgtgcacgc
     2761 tcttcgcggt cgcggaggag ctctatccgc gactttcgtc gttctgcctc gtgaacgtga
     2821 gcggcgaggc cacggagttc ggcatcgcca ccgagggcac gcttaccgac tccatcttca
     2881 taccgaaggg gacgaacggc ttcatgaaga aggtcatgga ggataccggc cgaccgctcg
     2941 ccgacgtcga atccgcgctt cgcgcgttcg gggcgaagga tgccgcgatg gcgtccgacc
     3001 tcgaagcgca tttgagcga
//
LOCUS       DASTSP010000054         7664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_110163, whole genome shotgun sequence.
ACCESSION   DASTSP010000054 DASTSP010000000
VERSION     DASTSP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110163"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2237
                     /locus_tag="VFS75_02410"
     CDS             <1..2237
                     /locus_tag="VFS75_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677548.1"
                     /translation="IVSYAVVEFTGKNWAVQRSEHAYTATGTAETEAITSLNSLSHAF
                     LYAQKRSGAGLSGVDEYGHEVWLSSIGAVSYYLEPGATNPSDQVSVAWVIENTETGTG
                     EMSVTRSNGTSNGGASPLTLSVSIGSTLTNASNASIFINGRSSGTDTNYPRPIAGATI
                     ASTTAYELWRSETGSTLSYRTEIVEWPTANLSIHQDYYRFYVDANALTPTDPWPLGAA
                     DLGENAVLTSTDEPLGGGERVRIRMSLQAVGATLPASSRSFKLQYGLMTTTCSAIPEQ
                     NWQTVGGTSSSTAWRGYDAAGAADGSVLSGDPPTAGDLLLSVSDVAGSYEETNDTAVN
                     SYAVPAGSDIEYDWLVEEHAASADSYYCFRMIESDGTPLTAYDNYPELKTSPFEPRSQ
                     NWRWYDDELDETPTTTLAAEGVAPSDVPTGNVLKLRVTVAETKNIARDDARFRLQYSE
                     YSDFRDAADVLSQAACTGSSTWCYANGGGADNAVITTGTLSDADACALSVGTGCGTHN
                     ESPDAALGFTHPAGASVEYEFTVKAVAPRVNSTYFFRLYDVDADAPVPVNAGESYPSL
                     ATEGAKLVFTMTGLAASAVVEGVTLDVTTTPTTIDFGTLPRGQLIEGAHRLTVDANGT
                     EGYKLYMLMTGDLTTTSGATIKEVTGTNGAPLSWNGGCAADAPSCFGYHTSDDTLADG
                     STRFSAIDTYARLSTTTPEEVAYSSQPVAGDFTDIVYRILVRGLQDAGNYETNIMYIS
                     VPVF"
     gene            2239..3234
                     /locus_tag="VFS75_02415"
     CDS             2239..3234
                     /locus_tag="VFS75_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677549.1"
                     /translation="MRRTPLTIVLLVALLLPALHANAAIAYKVEPLVIDLSAKARDIF
                     TKTITVTNTGTQPVTIFPTVNNISVADGGTIEEFLSPSASDRTSSLASWIEIPRLGVN
                     LKQGESKTFNVTFRLNPKPVPGTYHALIGFGYGRNQDEAQALVRAGRAPATMVTMDVE
                     SNRTEFLKLAGFVVDRFVTSANNEAASFTFKNPGEDPETPTGEIILYDGTGREVGSVP
                     VNAENVSVPPGGEHTFTASVPTDGMFGKYKAYLSVEYGGSERATVQDTAFYYVMPLGK
                     LIPSALAVLLAALLVSWFVHRRYLDEGDRDDHGVLTVHVRDTVSDAKEHDINLKR"
     gene            3231..4541
                     /locus_tag="VFS75_02420"
     CDS             3231..4541
                     /locus_tag="VFS75_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677550.1"
                     /translation="MIRVIFMRRALAFAALLATLFAPVVALAQTSQMMSVTPPLFQLS
                     AKPGDVWQSSIKVVNGNAYPLTVYAEVVDFRAVGEEGQGTFVPLTDDADGSTLASWID
                     IPEGPHVIEPEKTKDIPFVVDIPKDAPPGGHYAAILTTTKPPAKAAGESAVFTSQAVT
                     SLFFVRIEGDVREAGDIREFSVENAVVPKPEATFSVRFENKGNVHLQPRGDIVITNMW
                     GTERGVIPINNDTQFGNVLPESIRNFTFTWQRDLSFTDVGRYRALVTLAYGENGVKSV
                     SSITYFWVIPVKITLITLTILAFFIALIVWMVKVYVRRMLALAGIPVSGDVSRAGNAS
                     RDGSVHVPSYRKVSAPIRSGVLDLRTRLSSAPETKSTTSTLISFVVEYKAFFVSLLIL
                     ILIGVTAVLYIGRARTTDDDYRVIIKQGDETRTIEGKDVEGGAR"
     gene            4585..5061
                     /locus_tag="VFS75_02425"
     CDS             4585..5061
                     /locus_tag="VFS75_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677551.1"
                     /translation="MEKRNTIIAIVLIVIVVAGMFLYARMKRAEINAPAPEPAATTTD
                     VTPSPYAGITRIDAKHFFDGTTHTVVGSMSMPTPCDLLNWSTHADGSTVTIDFTVINN
                     AEMCAQTVTEARFKVAFDAPENATIKATLEGRPIDVNLIPAAPGETPDDYEIFIKG"
     gene            5063..5611
                     /locus_tag="VFS75_02430"
     CDS             5063..5611
                     /locus_tag="VFS75_02430"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4677552.1"
                     /translation="MREYVRGHVGFIAAIVATVAVLGFLTYSFVAPSGMFSGLQNDLK
                     ALDEGDGGTYTDLDGNPVPLSRFKGKPLIVNLWASWMPFSKDELSLMSALASERGESV
                     AVVAVNRMEDPATVRSYLATFGLGQGITVLLDPADSFYKKVGGYAMPETLFYAKDGTL
                     VEHKRGVLTEAELKAYVDSVAR"
     gene            5730..5879
                     /locus_tag="VFS75_02435"
     CDS             5730..5879
                     /locus_tag="VFS75_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677553.1"
                     /translation="MFRSIGTVLVIWYLSLLFAHSFRALDDALAASFRSLENVATVTG
                     ARTSR"
     gene            5959..7005
                     /locus_tag="VFS75_02440"
     CDS             5959..7005
                     /locus_tag="VFS75_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4677554.1"
                     /translation="MELRTDKRTIEYVYFFVVLAVSGYLVWQLVAPVISAVALAAIVA
                     TICYPMYERLLPRMPRKSPTLAALVSLLIVLIVIVIPLVLLSSLLLREAISIYTLFSQ
                     GDRLSIVEPLNQLNRAVQTFIPTFSIDAVSVVQQAAQFFVNNLVSIFAGTASTIFLFF
                     IALIAVFYFFRDGRYFTAYLVSMSPLSDDNDTLILEKLARAVRSVALGSVFVAIIQGI
                     LTAIGLSLFGFDRAILWGVVASVGALVPSVGTSVVFIPSVIYLFMTGQTMSALGVALW
                     GLLAVGLIDNLLGPYLMSRGNHMHPLVLLLSVLGGVALFGPIGFVLGPVVMSLFLVLL
                     ELYSTHFLDKENPV"
     gene            7019..>7664
                     /locus_tag="VFS75_02445"
     CDS             7019..>7664
                     /locus_tag="VFS75_02445"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU4677555.1"
                     /translation="MLAHLMGAVVGAFIAVAVSIFGPIREGAIVPASIPAPQEAPSPR
                     EAPRILFVGDVMLGRRVETLANEYGASYPFRHVSDEVALADYAVANFEGTVSDPHVHT
                     PDLTYRFSVSEDLFREVKGSGFDAISLANNHSLDYGVPAFLNTTRLCERYGIVCGGDA
                     SSPNLASTTVARIGGERVGFLYLVAIGDDPVDIESVRSLMDELRGASDFQVALVH"
BASE COUNT         1407 a         2531 c         2267 g         1459 t
ORIGIN      
        1 ccatcgtgag ctacgcggtc gtcgagttca ccgggaagaa ctgggccgtt cagcggagcg
       61 agcacgcgta caccgcgacg ggaaccgccg agaccgaggc gatcacgtca ctgaatagcc
      121 tttcccatgc gtttttgtac gcacagaagc gctcgggtgc gggtctctcc ggcgtcgacg
      181 agtacggtca cgaagtgtgg ctctcgtcga tcggcgcggt atcctattac cttgaaccgg
      241 gtgcgacgaa cccgagcgac caggtatcgg tcgcgtgggt catcgagaac accgagacgg
      301 gcaccggcga gatgtcggtg acgcggtcga acggcacctc gaacggtggt gcatcgccgc
      361 tcacgctctc cgtatcgatc gggagcacgc ttacgaacgc ctcgaacgcg tccatcttca
      421 taaacgggcg atcgtccggg accgacacga actatccgcg ccccatcgcc ggggcgacga
      481 tcgcctcgac gaccgcatat gaactttggc ggagcgaaac gggttcgacg ctctcgtatc
      541 ggacggagat agtcgaatgg ccgacggcga acctttccat ccatcaggat tactaccgtt
      601 tctacgtcga tgccaatgcg ctgacgccga ccgacccctg gcctctcggc gccgccgatc
      661 tcggcgagaa cgccgttctc acgtcgaccg acgaaccctt aggcggcggt gaacgcgtcc
      721 gcatccgtat gtcgcttcag gccgtcggtg cgacgctccc cgcgtcgtca cggtcgttca
      781 aactccagta cgggctcatg acgacgactt gtagcgcgat accggaacaa aactggcaga
      841 ccgtcggcgg gacgtcatct tcgaccgcgt ggcgcggcta cgacgcggcg ggagccgcgg
      901 acggctcggt cctctcgggg gacccgccga cagcgggaga cctccttctc tccgtgtccg
      961 acgtcgccgg ctcgtacgag gagacgaacg acacggccgt taactcgtac gccgttccgg
     1021 cggggagcga catcgagtac gactggctcg tcgaagagca tgcggcgagc gccgactcgt
     1081 actactgctt ccgcatgatc gagtcggacg gtacgccgct tacggcgtac gacaactatc
     1141 cggagctcaa gacatcgccg ttcgagcccc gctcacagaa ctggcggtgg tacgatgatg
     1201 agctcgacga gacgccgacg acgacgctcg ccgccgaggg tgtcgcacca tcggacgtgc
     1261 cgactggcaa cgttctcaaa cttcgggtca ccgtcgccga gacgaagaac attgcacggg
     1321 acgacgcgcg gttccgactc cagtactccg agtactcgga cttccgtgac gcggccgacg
     1381 tgctctcaca ggccgcatgt acgggtagtt cgacgtggtg ttacgcgaac ggcggaggag
     1441 cggacaacgc cgtcatcacg accggcacgc tctccgacgc cgatgcgtgc gctcttagcg
     1501 tcggtaccgg ctgtggaacg cacaacgagt cgcccgatgc ggcgctcgga ttcacgcacc
     1561 cggcgggcgc ttcggtcgag tacgagttca ccgtgaaggc ggtcgccccg agggtgaaca
     1621 gcacgtactt ctttcggctc tacgacgtcg acgccgacgc gccggttcca gtgaacgccg
     1681 gggaaagtta cccttcgctc gcgaccgagg gcgcgaagct cgtcttcacg atgacgggac
     1741 tcgccgcgtc cgccgtcgtc gagggagtga cgctcgacgt gacgacgacg ccgaccacga
     1801 tcgacttcgg aacgctcccg cgtggacagc tcatcgaggg agcgcaccgc ctcaccgtcg
     1861 acgcgaacgg caccgaggga tacaaactct acatgctcat gacgggcgac ctcacgacga
     1921 cgagcggcgc gacgatcaag gaggtaaccg gcacgaacgg cgcgccgctc tcatggaacg
     1981 ggggatgcgc cgccgacgcg ccgagctgtt tcggctacca tacgagcgac gacacgctcg
     2041 ccgatgggtc gacgcgtttc tcggcgatcg acacgtacgc gcgactctcg acgacgacgc
     2101 ccgaagaggt cgcgtacagt tcacagccgg tcgcgggtga tttcaccgac atcgtctacc
     2161 gcatcctcgt ccgtggtttg caagacgcgg gtaactatga gactaatatt atgtacatct
     2221 ccgtacccgt cttctgatat gcgccggaca cctctcacaa tcgtccttct cgtcgctctc
     2281 ttgctccccg ccctgcatgc caatgccgcg atcgcgtaca aggtcgagcc gctcgtcatc
     2341 gacctctccg cgaaggcccg cgacatcttc acgaagacta tcaccgtcac gaacacgggt
     2401 acgcaacccg tcaccatttt tccgacggtc aacaatatct cggtcgcgga cggtggcacc
     2461 atcgaggagt tcctctcacc gtcggcgtcc gaccgaacgt catcgctcgc ctcgtggatc
     2521 gagatcccgc gcctcggcgt caatttgaag cagggggaat cgaagacgtt caacgtcacg
     2581 ttccgtctca accccaagcc cgtcccggga acgtaccatg cgctcatcgg cttcggatac
     2641 gggcgcaacc aagacgaggc acaagcgctc gtgcgcgcgg gaagagcgcc agcgacgatg
     2701 gtgacgatgg acgtcgagtc gaaccggacg gagttcctca agctcgcggg attcgtcgtc
     2761 gaccgattcg tcacctcggc gaacaacgag gcggcatcct ttacgttcaa gaacccggga
     2821 gaggacccgg agacgccgac gggggagatc atcctctacg acgggaccgg cagggaggtc
     2881 ggctccgtgc cggtcaacgc cgagaacgta tcggtcccgc ccggaggcga gcataccttc
     2941 accgcgtccg tgccgaccga cggcatgttc gggaagtata aggcgtacct ctcggtcgag
     3001 tacggcggta gcgagcgggc gacggtccag gatacggctt tctattacgt gatgccgctt
     3061 gggaagctca ttccgagcgc gctcgccgtc ctcttagcgg cgctcctcgt ctcgtggttc
     3121 gtccaccggc gctacctcga cgagggagac cgcgacgacc acggcgtcct caccgtccac
     3181 gtacgcgata cggtgagcga cgcgaaggag cacgacatca atctcaaacg atgatacgcg
     3241 tcattttcat gaggagagcg ctcgccttcg ccgcactact cgcgacgctc ttcgcgccgg
     3301 tcgtcgccct ggcgcagacg tctcaaatga tgagcgtgac gccgcccctt tttcagctct
     3361 ccgcgaagcc cggggacgtc tggcagagca gcatcaaggt cgtgaacggg aacgcgtacc
     3421 cgctcaccgt ctacgcggag gtcgtcgatt tccgcgcggt cggtgaggaa gggcagggga
     3481 cgttcgtacc gctcacggac gacgccgacg gttcgacgct cgcatcgtgg atcgacatac
     3541 cggaaggacc gcacgtcatc gagccggaga agacgaagga cattcctttc gtcgtcgaca
     3601 taccgaagga tgcgcccccc ggcggccact acgccgccat cctcacgacg acgaagcctc
     3661 cagcgaaggc ggctggtgaa tccgcggtct tcacgtcgca agccgtcacg tccctcttct
     3721 tcgtccgcat cgagggtgac gttcgcgagg ccggcgacat acgtgagttc agcgtcgaga
     3781 acgccgtcgt tccgaagccg gaggcgacct tctccgttcg attcgagaac aagggcaacg
     3841 ttcacctcca gcctcgcggc gatatcgtca tcacgaacat gtgggggacc gagcgcggcg
     3901 tcatccccat caacaacgac acgcagttcg gcaacgtgct ccccgagagc atcaggaact
     3961 tcaccttcac gtggcagcgc gatctgtcgt tcacggacgt cggccgctat agggcgctcg
     4021 tgacgctcgc gtacggtgag aacggcgtga agagcgtgag ctcgataacc tatttttggg
     4081 tcattcccgt gaagataacg ctcatcacgc tcaccatcct cgcctttttc atcgcgctca
     4141 tcgtttggat ggtgaaggtg tacgtccggc gcatgctcgc cctcgcgggt ataccggtct
     4201 cgggggacgt gtcgcgcgct gggaatgcct ctcgtgacgg ttcggtgcat gtgccctcgt
     4261 acaggaaggt ctccgctccc atacgaagcg gcgtcctcga tcttcgcacg aggctctcct
     4321 ccgctcccga gacgaagagt acgacctcaa cgctcatttc gttcgtcgtc gaatacaaag
     4381 ccttcttcgt gtcgctcctc atcctcattc tcatcggcgt caccgccgtt ctctacatcg
     4441 gccgcgcgcg gacgaccgat gacgactatc gggtcatcat caagcagggt gacgagaccc
     4501 gcaccatcga gggcaaggac gtcgagggag gcgcgagata ggcgtggtat actggtacct
     4561 cattacgagg gtaacaataa cgttatggaa aaacgtaaca ccatcatcgc gatcgttctc
     4621 atcgtcatcg tggtagcagg catgttcctc tacgcgcgca tgaagcgagc ggagatcaac
     4681 gccccggctc ccgagcccgc ggcgacgacg accgacgtga cgccgtctcc gtacgccggc
     4741 atcacgcgca tcgacgcaaa gcatttcttc gacggcacga cgcacaccgt cgtcggttcg
     4801 atgtccatgc cgacgccgtg cgatctcctc aactggtcga cacacgccga cggctcgacc
     4861 gtgacgatcg acttcaccgt catcaacaac gccgagatgt gcgcgcagac ggtgaccgag
     4921 gcgcgcttca aggtggcgtt cgacgcgccg gagaacgcga ccatcaaggc gaccttagag
     4981 ggccgcccca tcgacgtgaa cctcatcccc gcggcgcccg gagagacgcc tgacgactac
     5041 gagatcttca tcaaggggta acatgcggga gtacgttcga ggtcatgtcg ggttcatcgc
     5101 cgccatcgta gcgacggtcg ccgtgctcgg tttcctcacg tactcgttcg tcgcgccgag
     5161 cggcatgttc tccggactcc agaacgacct caaagcgctc gacgagggcg acgggggtac
     5221 gtacaccgac ctcgatggga accccgtccc gctctcgcgc ttcaagggga agccgctcat
     5281 cgtgaacctt tgggcgtcgt ggatgccatt ctcgaaggac gagctttcgc tcatgagcgc
     5341 tctcgcgtcg gagcggggag agtcggtcgc ggtcgtcgcg gtgaaccgca tggaagaccc
     5401 ggcgacggtg cgctcgtacc tcgcgacgtt cggactcggg cagggcatta cggtcctcct
     5461 cgatcccgcg gactccttct acaagaaggt gggcgggtac gcgatgcccg agaccctctt
     5521 ttacgcgaag gacggtacgc tcgtcgaaca caagcgcggc gtactcaccg aagcggagct
     5581 caaggcctac gttgatagcg tcgcacggta gagttgcaaa gaccactaac gtgtggcata
     5641 gtttccgtag ggcagggaac caggcgacca ccggtattac cctgggctac gtgtttgtca
     5701 cacgtagtga atgtcgcata tcacccgcca tgtttcgatc tatcggaacc gtgctcgtta
     5761 tctggtacct ttcgctcctc ttcgcccact cgtttcgcgc gctcgacgac gcactcgcgg
     5821 catcgttccg atcgctcgag aacgtagcga cggtgactgg agcgcgaacc agcagatagg
     5881 atcaaggcca gacgtcgtct ggcctttgtt cgtattcgat acctttgttc cccgagccgt
     5941 acggtataca atggcgacat ggaactccgc accgataagc gcaccatcga gtacgtctac
     6001 ttcttcgtcg ttctcgccgt ctccggatac ctcgtgtggc agctcgtcgc accggtcatc
     6061 tccgccgtcg cgctcgcggc catcgtcgcg accatctgct acccgatgta cgaacggctt
     6121 cttccgcgca tgccgcgaaa gagtccgacg ctcgcggccc tcgtatcgct cctcatcgtc
     6181 ctcatcgtca tcgtcattcc gctcgtcctt ctctcgtcgc tcctccttcg ggaagcgata
     6241 tcgatctata cgctcttctc gcagggggat cgactctcca tcgtcgaacc gctcaaccag
     6301 ctcaatcgtg ccgtacagac gttcatcccg acgttctcca tcgacgcggt gagcgtcgtc
     6361 cagcaggcgg cccagttctt cgtgaacaac ctcgtttcga tattcgccgg caccgcgtcg
     6421 accatcttcc tctttttcat cgcgctcatc gccgtcttct acttcttccg cgatggcaga
     6481 tactttacgg cctacctcgt ctcgatgagt cccttaagcg acgataacga tacgctcatc
     6541 ctcgagaagc tcgcccgtgc cgtccgctcg gtcgccctcg gctccgtctt cgtcgccatc
     6601 attcagggaa tcctcaccgc gataggcctg agcctcttcg gtttcgaccg cgcgatactc
     6661 tggggcgtcg tcgcgtcggt cggtgccctc gttccgtcgg tcggcacgag cgtcgtcttc
     6721 attccgtccg tcatctacct attcatgacc ggacagacca tgagcgccct cggcgtcgcg
     6781 ctctggggac tcctcgccgt cggcctcatc gacaatcttc tcggcccgta tctcatgagc
     6841 cgtggaaacc atatgcatcc gctcgtgctc ctgctctcgg tgttgggcgg cgtcgcgctc
     6901 ttcggtccga tcggcttcgt gctcggaccg gtcgtcatga gccttttcct cgttcttctc
     6961 gagctctaca gcacgcactt cctcgacaaa gagaatcccg tctgatacac cgtctgatat
     7021 gctcgcgcac ctcatgggag ccgtcgtcgg cgccttcatc gccgtagcgg tctctatctt
     7081 cggccctatc cgagagggtg ccatcgtgcc cgcatcgata ccggcgccgc aggaggcgcc
     7141 ctcgccgcgc gaagcgccgc gtattctctt cgtcggtgac gtcatgctcg ggcgccgcgt
     7201 cgaaacgctc gcaaatgagt acggcgcgtc gtacccgttc aggcacgtca gcgacgaggt
     7261 ggcgctcgcc gactacgcgg tcgcgaactt cgagggtacg gtgagcgacc cgcacgtgca
     7321 cacgcccgac ctcacgtacc gctttagcgt cagcgaggac ctctttcgcg aggtgaaggg
     7381 aagtggtttc gacgcaatct cactcgcgaa caatcacagc ctcgactacg gcgttcccgc
     7441 attcctcaac acgacgcgcc tttgcgagcg ctatggcatc gtgtgcgggg gcgacgcgtc
     7501 gtcgccgaac ctcgcgtcga cgacggtcgc gcgtatcggc ggcgagagag tcggttttct
     7561 gtaccttgtc gcgatcggcg acgacccggt cgatatcgaa agcgtgcgct cgctcatgga
     7621 cgagcttcgg ggagcgagcg attttcaggt cgcgctcgtc catt
//
LOCUS       DASTSP010000055         7584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_560245, whole genome shotgun sequence.
ACCESSION   DASTSP010000055 DASTSP010000000
VERSION     DASTSP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560245"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..791)
                     /locus_tag="VFS75_02450"
     CDS             complement(204..791)
                     /locus_tag="VFS75_02450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU4677556.1"
                     /translation="MQRLEERERELEQMKRRLKARERHIKRLQTELRTDHLTKLPNRQ
                     SFYEELVLEADRARRKKTSLYVVYFDIDHFKRINDTYGHAVGDKVLVKIGRILRRELR
                     SDEFSARIAGDEFAIILSSLRRDEDAERMLDRLRKKMERCLCVSVVDRTIFATASFGV
                     AKMRPSEKTDAFLVRVDDAMYTAKKRGRNVVHVSS"
     gene            complement(891..1790)
                     /locus_tag="VFS75_02455"
     CDS             complement(891..1790)
                     /locus_tag="VFS75_02455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU4677557.1"
                     /translation="MATLYTHQGENVAKTWLLMGAFLVLVVAIGYAVSWYLGSPAILY
                     GALIFAILMNVGSYWFSDRVVIAMTRARPATREAYPDLWNVVENLSITAGLPMPKVYV
                     VDDPAPNAFATGRNPEHAAIAATTGLLALLERSELEGVIAHELSHVGNRDMLVMTVAV
                     VLAGFIAMLANFFMRSLAWGGGRGDRDRNPLLAVFAIVGIILMPIAAQLIQLAISRKR
                     EFLADASGALLTRYPEGLASALEKLRAYSRPMTHANKATAHLFIADPYGGDNPSFKER
                     VSSLFQTHPRIEDRIKVLRGMDV"
     gene            complement(1831..3564)
                     /locus_tag="VFS75_02460"
     CDS             complement(1831..3564)
                     /locus_tag="VFS75_02460"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU4677558.1"
                     /translation="MMEATNAHTWKNGARGLIVLALFAFALSAPNAARAANEAIESFE
                     DDIAVSRDGSFSVSEDIVYTFAAERHGIYRCIPLTHPDQASSFFKERYLDVTIDSVTM
                     DGTAVPYTDERTGGRLCLTIGDPAGTVSGTHAYRVDYVVRGGLSYPDFGGAELYWNVT
                     GNEWTVPIKVVEARVSSPDGLLTRERACYAGTEAKTASCAISIDETGAANFKGTLFDP
                     GAGLTLAQGLSRSAVAYDVRERYRPIVFIGGSILLLLVAAAVFLYRYETKNRTGRTIV
                     PQYEPYPGLEPIYTGYLFDRRLDARDISAGFVRLAQEGYLTVKKRDAKVLFLFSVDDY
                     ELTLKRPIADAAGTVKEELLRLLFDEKASVGSTVTLAHLAKDTAKQRMNASRLRALRA
                     RLARDVRAMGYMESVDFTFIFKTRFIVALLVVAAAFAFIESALVIAPIVAFLVVLLIL
                     SAGRRTKKGYEALDQLRGFKEFLKVTESQRYIFHNAPARNAEQFMEFLPYAIAFGVER
                     EWAKTFEGITIPRPLWYEGGSEGAWSAMNFTASLGAFSGSLRGATAGTSASTGGGHVG
                     GGAGGGGGGSW"
     gene            complement(3572..3943)
                     /locus_tag="VFS75_02465"
     CDS             complement(3572..3943)
                     /locus_tag="VFS75_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU4677559.1"
                     /translation="MEKIYYNKLCRDKVPAIIKAKGFDCEVRQVDHDEYKREIIRKVF
                     EEASGVSNHSGREGLLKELADLMITVDAVKKEYGIADSELDSAIEKSIADKGGYDERY
                     YLSWSSDTEYNSTDQGRGFDD"
     gene            complement(3962..4549)
                     /locus_tag="VFS75_02470"
     CDS             complement(3962..4549)
                     /locus_tag="VFS75_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU4677560.1"
                     /translation="MSLTYIILIVIAVIALWVVLAYNGFVKRVQRTKEAWADIDVQLK
                     RRHDLIPNLVETVKGYAAHERETFDAVTNARTEAARVHVDPSALTPEGMQAMAGAENG
                     LSQALGRLFAVAEAYPDLKANTNFLELQRELSDTENKIQAARRFYNGNARDLKIALQS
                     FPSNVIGNLFHFREEPYFELGDGDTAKDPVQVSFK"
     gene            complement(4674..6320)
                     /gene="groL"
                     /locus_tag="VFS75_02475"
     CDS             complement(4674..6320)
                     /gene="groL"
                     /locus_tag="VFS75_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757603.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU4677561.1"
                     /translation="MAKEVIYGEDVKKRLKKGVDTVANAVKLTLGPRGRNVIIDKGYG
                     GPTITNDGVSIAKEITLADKFENMGAEIVKEVANKTNELAGDGTTTATILTQALLAEG
                     LKHTTMGVNAMAVRSGMDKAAGDIVRALKELAVKITTTEEVKQVATISAESEEIGAKI
                     AETIDKVGKDGVVTVEESQSFGIDTEFTEGMEFDRGYVSPYMITNPERMEAEYKDALV
                     LVTDGKISTAQQIIPLLEKVAQTGKKEIVIIADDVEGEALATFVLNKIRGGFSVLAVK
                     APGYGDRKKDMLEDIAILTGGSVITEQVGLKLESAELSHLGKAARVVSTKDKTVIVGG
                     EGKKEAIDARVAALRSQLTATDSKFDKEKLEERIAKLSGGVAVIKVGAATETEMKYLK
                     LKIEDAVNATKAAIEEGIVPGGGTSLSRAAAVVEGQVDKELDRDELIGYRIVLKAMEE
                     PLKQLANNVGKHDGAVVVERVKEKGGNFGYNAQKAEYEDDMIKAGIIDPVKVERAGVE
                     HAVSAVGILLTSEVAVADAPEPEKPAAPDMSGMGGMGGMM"
     gene            complement(6443..6769)
                     /locus_tag="VFS75_02480"
     CDS             complement(6443..6769)
                     /locus_tag="VFS75_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU4677562.1"
                     /translation="MTSKAGEGSTIRPLQDRVVVRRLTDEEAGTKSASGIIVPDTVSK
                     EKPEQGIVTAVGDGKWDEKGEKRVPMDVKVGDRIVFSKYGYDEVKVGGEEYFIVSASS
                     ILGVIA"
     gene            6915..>7584
                     /gene="pyrE"
                     /locus_tag="VFS75_02485"
     CDS             6915..>7584
                     /gene="pyrE"
                     /locus_tag="VFS75_02485"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU4677563.1"
                     /translation="MDASSFIAYALSQTAIEFPRGGRRLRSGRVSPYFFNVGRFDTAY
                     ALGKLAEAYASAILTAVTVETSTHPEVIFGPAYKGIVLAAQTAIQLARIYYPFRERLG
                     YAYNRKERKNHGEGGVMVGMSLRGKRVVIVDDVISSGGSGDEAVALVRREGGVPIGYV
                     IGLDRLESDPESGTSVSETFTARHGIPVRAVSTVRDLTSFLERNPEYPNAADVRRRIE
                     EYLGL"
BASE COUNT         1338 a         2467 c         2260 g         1519 t
ORIGIN      
        1 gggagaagga acatcgcgat cgtcgcgatg actgtccaaa ggaaatactt cctcgtcttc
       61 tccgcggacg cgtacaccgc gtcgatcttc tcctcgaggc gcgcgagcgt ctcctgtggc
      121 gtttcgtttg gcataccgac atgataccac ctcgccccgg cgaggggtaa aaaaagggcg
      181 acctggcggt cgccctcgtt cgatcacgac gaaacgtgaa ccacgttccg ccctcgcttc
      241 ttcgccgtgt acatcgcgtc gtcgacgcga acgaggaacg catcggtctt ctccgaggga
      301 cgcatcttcg cgactccgaa gctcgcggtc gcgaagatgg tccgatcgac cacggagaca
      361 cagagacagc gctccatctt cttccggagc cgatcgagca tccgctcggc atcctcgtcg
      421 cgccgaagcg acgagaggat gatggcgaac tcgtcccccg cgatgcgcgc ggagaactca
      481 tcggagcgga gctcacgccg gaggatccga ccgatcttca cgagaacctt gtcaccgacg
      541 gcgtggccgt acgtatcgtt gatcctcttg aagtggtcga tatcgaagta gacgacgtac
      601 aaggacgtct tcttccgtcg cgcccggtcg gcctcaagga cgagctcctc gtagaaggat
      661 tgccggttcg gcagcttcgt gaggtggtcg gtgcgaagct cggtctgaag ccgtttgata
      721 tgccgttcgc gagccttgag cctccgcttc atctgctcga gctctcgctc gcgctcttcg
      781 agccgctgca cgagcagggc tttgtcggtt tcgagtatta ccacccgtcc ctcccttagg
      841 gtcgttgtct acccattatt atgacattta tggatgtcaa agtcaataga ctacacgtcc
      901 attccccgga gaaccttgat gcggtcctcg atacgcgggt gcgtctgaaa aaggctcgaa
      961 acacgctctt tgaaagacgg attgtctccg ccgtacgggt cggcgatgaa gaggtgtgcc
     1021 gtcgctttgt tcgcgtgcgt catcgggcgc gagtacgcgc ggagcttctc cagggcggac
     1081 gcgagcccct cggggtatcg cgtgaggagg gcacccgaag cgtcggcgag gaactcgcgc
     1141 ttccgcgata tcgcgagttg gatgagctgt gcggcgatcg gcatgaggat gatgccgacg
     1201 atcgcgaaga cggcgaggag cgggttacgg tcgcggtccc cgcgtccccc accccacgcg
     1261 agggaacgca tgaagaagtt cgcgagcatg gcgatgaagc cagcgaggac gacggccacg
     1321 gtcatgacga gcatatcgcg attcccgacg tgcgagagtt catgcgcgat gacaccttcg
     1381 agctcgctcc gttcgaggag cgcgaggagt cctgtcgtcg ccgcgatcgc cgcgtgttcg
     1441 gggttccgcc cagtcgcgaa cgcgttcggc gccgggtcat cgacgacgta caccttcggc
     1501 atgggaagcc ccgcggtgat ggagaggttc tcgacgacgt tccagaggtc cggatacgcc
     1561 tcacgcgtcg ccggcctcgc tcgcgtcatc gcgatgacca ccctatcgga gaaccagtaa
     1621 ctcccgacgt tcatgaggat cgcgaagatg agggcgccat agagaatggc cggcgaaccg
     1681 aggtaccatg agacggcgta gcctatcgcg acgacaagga cgaggaacgc acccatgagg
     1741 agccacgtct tcgcgacgtt ctcgccttga tgcgtataga gagtagccat atgaagattg
     1801 tatccgtgaa aaatgttctg taaatgaagg tcaccagctg ccgcctcccc cgccgccagc
     1861 cccgcccccg acgtgcccac ctcccgtaga tgcgctcgtc cccgcggtcg cgccccggag
     1921 cgaccccgag aaggcgccga ggctcgccgt gaagttcatc gcgctccacg ccccctcgga
     1981 cccgccctcg taccagagcg gcctcggtat cgtgatcccc tcgaaggtct tcgcccactc
     2041 tcgctcgacg ccgaacgcga tcgcgtacgg gagaaactcc atgaactgct cggcgttccg
     2101 cgcgggcgcg ttgtggaaga tgtaccgctg ggactccgtc accttgagga actccttgaa
     2161 accgcgtagc tggtcgagtg cctcgtatcc cttcttcgtc cgccgtcccg cggagaggat
     2221 gagaagaacg acgaggaacg cgacgatggg cgcgatgacg agcgcgctct cgatgaacgc
     2281 gaatgcggcc gcgacgacga ggagcgcgac gatgaagcgc gtcttgaaga tgaacgtgaa
     2341 gtcgaccgac tccatgtagc ccatcgcccg tacgtcgcgc gcgagccgcg cgcggagcgc
     2401 tcgcagacgg gacgcgttca tgcgctgttt cgccgtgtcc ttcgcgagat gcgcgagggt
     2461 cacggtcgac ccgaccgacg ccttctcatc gaagagtagc cgaaggagct cctccttcac
     2521 cgtccccgcc gcatcggcta ttggccgctt gagggtaagc tcgtagtcat cgaccgaaaa
     2581 gaggaaaagc actttcgcgt cgcgcttctt cacggtgagg tacccctctt gtgcgagacg
     2641 cacgaagcct gcggagatat cgcgcgcgtc gagccgccgg tcgaagaggt atcccgtgta
     2701 gatcggctcg agaccagggt acggctcata ctgcggcacg atggtccgtc ccgttcgatt
     2761 cttcgtctcg taccggtaga ggaagaccgc cgcggcgacg aggagcagaa ggatgctccc
     2821 gccgatgaag acgatggggc ggtatcgctc tcgaacgtcg tacgcgaccg cggagcggct
     2881 aagaccttgt gcgagcgtga gtcccgcccc aggatcgaaa agcgtgcctt tgaagtttgc
     2941 ggctcccgtc tcgtcgatcg atatcgcgca cgacgcggtc ttcgcctcgg ttcccgcgta
     3001 acacgcgcgc tcccgcgtga ggagcccgtc cggcgacgac acgcgggctt caacgacctt
     3061 gatgggaacg gtccattcgt ttcccgtcac gttccagtag agctcggcgc cgccgaagtc
     3121 ggggtacgaa agcccgccgc gaacgacgta gtcgacgcgg taggcgtgcg tacccgagac
     3181 cgttcccgca gggtctccta tcgtgaggca gagcctccct cccgttctct cgtcggtgta
     3241 cggaacggcg gtcccgtcca tcgtcaccga atcgatggtg acgtcgaggt atcgttcctt
     3301 gaagaacgac gacgcctggt ccgggtgcgt aagcggaata cagcggtaga tgccatggcg
     3361 ttccgcggcg aacgtgtaca cgatgtcttc ggagaccgaa aaggagccgt ccctcgacac
     3421 cgcgatgtcg tcctcgaacg attcgatggc ctcgttcgcc gcgcgcgcgg cgttcggcgc
     3481 ggaaagcgca aacgcgaaga gcgcgaggac gataaggccc cgcgcgccgt tcttccacgt
     3541 gtgtgcgttc gtcgcctcca tcatgcgtac gctaatcgtc gaatccgcgt ccctgatcgg
     3601 tcgaattgta ttcggtgtca gaagaccatg agagatagta ccgctcgtcg tatccgccct
     3661 tgtcggcgat gctcttctcg atagccgaat cgagctcgct gtccgcgatg ccgtactcct
     3721 tcttcaccgc gtcgacggtg atcatgaggt cggcgagctc cttgaggagt ccctcgcgtc
     3781 ccgagtggtt cgagacgccg ctcgcctcct cgaagacctt cctaatgatc tcccgcttgt
     3841 actcgtcgtg gtcgacctgg cgaacctcgc agtcgaaccc tttcgccttg atgatagcgg
     3901 gcaccttgtc cctgcagagc ttgttgtagt agatcttttc catggtgtgg agaggtgacg
     3961 attatttgaa actaacctga accgggtcct tcgcggtatc gccgtcaccg agctcgaagt
     4021 acggttcctc cctgaagtgg aacagattcc cgatgacgtt cgaggggaac gactggagcg
     4081 caatcttgag gtctcgtgcg ttgccgttgt agaagcgccg cgccgcctga atcttgttct
     4141 cggtgtccga gagctcgcgc tggagctcga ggaagttcgt gttcgccttg agatcgggat
     4201 acgcctccgc gaccgcgaag agtcggccga gcgcctgcga aaggccgttc tccgcgcccg
     4261 ccatcgcctg catgccttcc ggcgtgagcg cggatggatc gacgtggaca cgcgccgcct
     4321 cggtccgcgc gttcgttacc gcgtcgaagg tctcgcgctc atgcgcggcg taacccttca
     4381 cggtctcgac gaggttgggg atgaggtcat ggcggcgctt cagctgaacg tcgatatcgg
     4441 cccacgcctc cttcgtccgc tggacgcgct tcacgaagcc gttatacgcg aggacgaccc
     4501 agagcgcgat gacggcgatg actatgagaa tgatgtaagt gagtgacatg gctgttatcg
     4561 ttcgtggata atgacgactt cagtatagca cccggtgaag ctcttatgaa aagcgaaaaa
     4621 ccggcaccta cggtgccggt ttttctaagc tctagtcgct ctatacgcgg ccatcacatc
     4681 atccctccca tgccgcccat gcccgacatg tcgggcgcgg ccggcttctc cggttccggc
     4741 gcgtccgcga ccgcgacctc cgaggtgagg aggataccga ccgcggagac cgcgtgctcg
     4801 acgccggcgc gctcgacctt caccgggtct atgatgcccg ccttgatcat gtcgtcctcg
     4861 tactccgcct tctgcgcgtt gtacccgaag ttaccgccct tctccttcac gcgctcgacg
     4921 acgaccgcgc cgtcgtgctt gccgacgttg ttcgcgagtt gcttcaaggg ctcctccatc
     4981 gccttaagga cgatgcgata accgatgagc tcgtcgcggt cgagttcctt gtcgacctga
     5041 ccctcgacga ccgccgccgc gcgcgagagc gacgtcccgc cgcccggcac gatgccttcc
     5101 tcgatcgccg ccttcgtcgc gttgaccgcg tcctcgatct tcaatttcaa atacttcatc
     5161 tcggtctccg tcgccgcgcc gaccttgatg acggcgacgc cgcccgagag cttcgcgatg
     5221 cgctcctcga gcttctcctt gtcgaacttc gagtcggtcg cggtgagttg cgagcggagc
     5281 gccgcgacgc gcgcgtcgat cgcctccttc ttcccctcgc cgcccacgat gaccgtcttg
     5341 tccttcgtcg agacgacgcg cgccgccttc ccgaggtgcg agagttccgc gctttcgagc
     5401 ttgagaccca cctgctcggt gatgaccgaa ccgccggtga ggatggcgat gtcctcgagc
     5461 atatccttct tcctatcgcc gtagcccggc gccttcaccg cgaggaccga gaaaccgccg
     5521 cggatcttgt tgaggacgaa cgtcgcgagc gcctcgccct cgacgtcgtc cgcgatgatg
     5581 acgatctcct tcttccccgt ctgcgcgacc ttctcgagga gcgggatgat ctgctgcgcc
     5641 gtcgagatct tgccgtcggt gacgaggacg agcgcgtcct tgtactccgc ctccatgcgc
     5701 tccgggttcg tgatcatgta cggcgagacg tacccgcgat cgaactccat accctcggtg
     5761 aactccgtgt cgatgccgaa cgactgcgac tcctcgacgg tcacgacgcc gtcctttccc
     5821 accttgtcga tggtctcggc gatcttcgcg ccgatctcct cgctctccgc ggagatggtc
     5881 gcgacctgct tcacctcctc cgtcgtcgtg atcttcacgg caagctcctt gagcgcgcgg
     5941 acgatatcgc ccgcggcctt gtccatgccg ctacggaccg ccatcgcgtt cacgcccatc
     6001 gtcgtatgct tgagcccctc cgcgaggagc gcctgcgtga gaatggtcgc ggtcgtcgtg
     6061 ccgtcgcccg cgagctcgtt cgtcttgttc gcgacctcct tcacgatctc cgcgcccatg
     6121 ttctcgaact tgtccgcgag cgttatctcc ttcgcgatcg acacgccgtc gttcgtgatg
     6181 gtcggcccac cgtacccctt gtcgatgatg acgtttcgtc cgcgcgggcc aagagtgagc
     6241 ttcaccgcgt tcgcgaccgt gtcgacgccc ttcttgaggc gcttcttgac gtcctctccg
     6301 tatatcactt cctttgccat gcctagtagt tcttaatgag cgcagcgaat tttagaacga
     6361 cttggccgtc gtttcgatgg acccccgtcc ttcgaaacga cgccctgctc aaaaatcgcc
     6421 gcatagtaaa tattcgatga atctatgcga tcactcccaa aatgctcgac gccgagacga
     6481 tgaagtactc ctcgccaccg accttcacct cgtcgtagcc gtacttcgag aacacgatgc
     6541 ggtcccctac cttcacgtcc atgggaacgc gcttctcgcc cttctcgtcc catttcccgt
     6601 cgccgacggc cgtcacgatg ccctgttcgg gcttttcctt gctcacggtg tcggggacga
     6661 tgatacctga cgctgatttc gtgcccgctt cctcgtcggt aagccggcgg acgaccacgc
     6721 ggtcctggag cggacggatg gtcgaacctt cacctgcctt tgaagtcata tacaaatatt
     6781 cgttatcctt aacacctgat aatgcggggc tcattatagc gcggagcggc gcgctgtcca
     6841 gatttttaga aagttgcggc atccgtcgaa tgggtctatg ctctccgaag ccagtacctc
     6901 gagaaaggaa ccccatggac gcctcgtcct tcatcgcgta cgcgctctcc caaacggcca
     6961 tcgaattccc ccgaggaggg cgacgactcc ggagcgggcg cgtgagcccg tacttcttca
     7021 acgtcggacg cttcgacacc gcatacgcgc tcgggaagct cgccgaggca tacgcgtcag
     7081 cgatactcac cgcggtcacc gtcgagacgt ctacccaccc ggaagttata ttcgggccgg
     7141 cgtacaaggg catcgtcctc gccgcacaga ccgcgatcca gctcgcccgc atctactacc
     7201 catttcgaga aaggctcggg tacgcctaca acagaaagga gcgaaagaac cacggcgagg
     7261 gcggcgtcat ggtcggcatg tcgctacgcg gaaagcgcgt cgtcatcgtc gatgacgtca
     7321 tttcctcggg aggaagcggc gacgaggccg tcgcgctcgt gcggcgagaa ggcggcgtac
     7381 cgatcgggta cgtgatcggc ctcgaccgac tcgaaagcga cccggagagc ggaacgtcgg
     7441 tctcggagac atttacggca cggcacggca ttcccgtccg cgccgtatcg accgtccgcg
     7501 acctcacctc gtttctcgag cggaaccccg agtatccgaa cgccgccgat gtccggcggc
     7561 gcatcgagga gtacctcggg ctct
//
LOCUS       DASTSP010000056         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_561610, whole genome shotgun sequence.
ACCESSION   DASTSP010000056 DASTSP010000000
VERSION     DASTSP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561610"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFS75_02490"
     CDS             complement(<1..600)
                     /locus_tag="VFS75_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4677564.1"
                     /translation="MERDAHRRILLVGGGTGGHFYPLIAIAEAIAKGGEADIYYAGPS
                     RYDADALRKAGIGYIAVPAGKTRRYRSVLNVFDPFLTLIGALVALLRLYILYPDVVVS
                     KGGYTSVPVIIAAAFLRIPIIVHESDSVIGRANRLAIRFARLVIVSYDETIAQVAGKR
                     VEKLGIPVREELLAPVAQNAREALGIPAGRPLVFVTGGSQ"
     gene            complement(622..1722)
                     /locus_tag="VFS75_02495"
     CDS             complement(622..1722)
                     /locus_tag="VFS75_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEU4677565.1"
                     /translation="MHAPDRTLLFLTVALVAAGFLIFSSASLGLLARQGASFSSVAFS
                     QFFFGIVGGSIALFLTSSVHYRWWRQNAFYIFLATFVFTFTVFIPGLYFAHGGAHRWI
                     GVGPFTMQPAELLRVGYIIYMATWLSGMKQKIGSFTKGTLPFVGLVALVGIPMLLQPD
                     TDTYMIMVAAALAMFIAAGGRVRDVVLMTVLGVLLLSVLAMTRPYIKDRIHTFLDPSS
                     DPQGSGYQIKQSLIAIGSGGVLGRGFGQSIQKFEYLPEPIGDSVFAVYAEEFGFVGSV
                     VLILMFAAFTLRGYRIATHAPDLFGMLLVVGLTTLVIAQVSLNIAAMLAIAPLSGLPL
                     PFISHGGTALLVTLASIGIILNVSKYQRASVG"
     gene            complement(1724..2797)
                     /locus_tag="VFS75_02500"
     CDS             complement(1724..2797)
                     /locus_tag="VFS75_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677566.1"
                     /translation="MESTIIKVLAPMAVAFVVGMLITPFLTNYLYRYEIWKKKAGKGV
                     GYGGGETPLFDELHKHRDTNTPRMGGIVIWASVLITVLLVRLLSVLFGSHVVGALDFL
                     SRSQTWIPLAAMLVGASVGFLDDLLVVRGTGTHFAGGLPLSQRLLAVTVTAFLAGWWF
                     YDKLGVAEVNLIGHGPIHLGLWFLPFFVLVAIAIYASSVIDGIDGLSGGTFMFIFTAY
                     AGIAAYQNQIDLAAFCATVVGGILAFLWFNIPPARFYMTETGIMALTMALTIVVFMTD
                     ALGGGEGVSVFPVIGILLVLTVLSDVIQLASKKFFGRKVFRIAPLHHHFEALGWSAAK
                     VTMRYWVLGFVFAIIGTILAISF"
     gene            complement(2802..3854)
                     /locus_tag="VFS75_02505"
     CDS             complement(2802..3854)
                     /locus_tag="VFS75_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4677567.1"
                     /translation="MIPEHKDEERDDAVSELFSDETAESPKHVSTIPVVPQLSVALGI
                     LVFVFGVTYVGASHSLKEQSAAADEVRVDTTLADRSHDEAARVGAFDDVSLEAKGAIV
                     WDVKNQKALYAKNADESLPLASVTKLMTALLTYELLSPEDRVTITRRAIEVEGDSGFA
                     DGEEFTMQDLADLTLITSSNDGATALGARTGEVLSSSNPDAAFVEAMNVRAEELGLTQ
                     TSFKNPTGLDLSPTEPGALGSARDVARLMDFIVTNVPDAVALTNVDLTTIDNARGQYH
                     IAKNTNIDVATIDGLIASKTGYTDLAGGNLVVAFNAGLNRPVVVAVLGSSFNGRFADV
                     SDLVARTRRYVSESSR"
     gene            complement(3851..>4351)
                     /locus_tag="VFS75_02510"
     CDS             complement(3851..>4351)
                     /locus_tag="VFS75_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU4677568.1"
                     /translation="LLSVSLEDIETALGALQESLRDRGVRLVTTDTEAQLVTAPELSE
                     FIDGIRKDELEKEIGRAGAETLAIVLYRGPLSRAEIDRIRGVNSAFILRNLLIRGLVE
                     RRAHPTDARSFVYAATSDLLNHLGIDRRESLPDFGEIMNALDAFEKQADEAETTGDDE
                     TSPFRS"
BASE COUNT          856 a         1260 c         1487 g          748 t
ORIGIN      
        1 ctgggaacca ccggtcacga aaacgagcgg acggcctgcg gggatgccga gggcctcgcg
       61 tgcgttttgc gctaccggcg cgaggagctc ttcgcggacc ggtatgccga gcttctcgac
      121 gcgtttcccc gccacctgag cgatcgtctc gtcgtacgac acgatgacca agcgcgcgaa
      181 gcggattgcg aggcgattcg ctcgtccgat gacggagtcc gattcgtgca cgatgatggg
      241 tatccgaagg aacgccgcgg cgatgatgac gggaacgctc gtgtagccgc ccttacttac
      301 gacgacgtcg ggatagagga tgtagagccg aaggagcgcg acgagagcgc cgatgagcgt
      361 gaggaacggg tcgaagacat tgaggacgga acggtaccgc ctggtcttcc ccgccggaac
      421 ggcaatgtac cctatccccg ctttcctaag cgcgtcggcg tcgtatcgag acgggccagc
      481 gtagtagatg tcggcctccc cgcccttcgc aatggcctcc gctatcgcga tgagcggata
      541 gaaatgaccg ccggtaccgc cgcccacgag aagtatgcgt ctgtgcgcgt cgcgttccat
      601 atactttgag attgtagcac gctagccgac gctcgctcgc tggtatttcg agacgttgag
      661 tataatcccg atcgatgcga gcgtgacgag gagcgccgtt ccgccgtgac tgatgaacgg
      721 aagcgggagt ccggagagcg gcgctatcgc gagcatggcc gcgatgttga gcgagacttg
      781 cgcgatgacg agcgtcgtca agccgacgac gaggagcatg ccgaagaggt cgggcgcgtg
      841 cgtcgcgata cggtagccgc gaagcgtgaa cgcggcgaac atgaggatga ggacgacgga
      901 gccgacgaag ccgaactcct cggcgtacac ggcgaagacc gagtcgccga tcggttcggg
      961 gaggtactcg aacttctgga tgctctgacc gaatccacgg ccgaggacgc cgcccgagcc
     1021 gatcgcgatg agggattgct ttatctggta ccccgaacct tgcgggtcgg agctcgggtc
     1081 gagaaacgtg tgaatgcggt ctttgatgta cgggcgcgtc atcgcgagga ccgagaggag
     1141 gagtacaccg aggacggtca taaggacgac gtcgcggacg cggccgcccg ccgcgatgaa
     1201 catcgcgagc gcggccgcga ccatgatcat gtacgtgtcg gtatctggct gaaggagcat
     1261 cgggatgccg accaacgcga cgaggccgac gaacgggagc gttcccttcg tgaagctccc
     1321 tatcttttgc ttcatgccgg agagccacgt cgccatgtag atgatgtacc cgacgcggag
     1381 gagctccgcg ggctgcatcg tgaacggtcc gacgcctatc cagcggtgcg cgccgccatg
     1441 cgcgaagtag agtccgggaa tgaagaccgt gaacgtgaag acgaacgtcg cgaggaatat
     1501 gtagaacgcg ttctggcgcc accaccggta atgcacggac gacgtgagga agagcgcgat
     1561 actcccaccc actatgccga agaagaactg gctaaaggcc accgacgaga agctcgcgcc
     1621 ctgtcgcgcg aggagaccga gggacgccga ggagaagatg aggaaccccg ccgcgacgag
     1681 cgcgacggtg agaaagagga gcgtgcggtc gggagcgtgc atatcaaaac gagatggcga
     1741 ggatcgtgcc gatgatggcg aagacgaagc cgaggaccca atatcgcatc gtcaccttcg
     1801 ccgcggacca gccgagcgcc tcgaagtggt ggtgaagcgg cgcgatgcgg aataccttcc
     1861 tcccgaagaa cttcttcgag gcgagctgta tgacgtccga gagcacggtg agaacgagga
     1921 ggatgccgat gaccgggaag accgagaccc cctctccccc gccgagcgcg tcggtcatga
     1981 agacgacgat ggtgagcgcc atcgtgagcg ccatgatgcc cgtttccgtc atgtagaagc
     2041 gcgcgggcgg gatattgaac cagaggaacg cgagaatgcc tccgacgacg gtggcacaaa
     2101 atgcggcgag gtcgatctgg ttctggtacg cggcgatgcc agcgtacgcg gtgaaaatga
     2161 acatgaacgt gcctcccgaa agcccgtcga tgccgtcgat cacgctcgat gcgtagatgg
     2221 cgatcgcgac gaggacgaag aacggcagga accagagccc gaggtgaatg gggccatggc
     2281 cgatcagatt gacctccgcg acgccgagct tatcgtagaa ccaccagccg gcgaggaacg
     2341 ccgttaccgt gaccgcaagg aggcgctgtg agagcggcag gccgcccgcg aagtgcgtcc
     2401 ccgtgccgcg gacgacgagg aggtcgtcga ggaatccgac cgaggcgccg acgagcatcg
     2461 ccgcgagcgg gatccacgtc tgcgagcggc taaggaagtc cagggcgccg acgacgtgcg
     2521 aaccgaaaag aacgctaagg agccggacga ggaggaccgt gatgagaacg ctcgcccaga
     2581 tgacgatacc gcccatgcgc ggcgtgttcg tgtcgcggtg cttatggagc tcgtcgaaga
     2641 gcggtgtctc gccgccgccg tacccaacgc ccttccccgc cttcttcttc cagatctcgt
     2701 agcggtagag atagttcgta aggaacggcg tgatgagcat gccgacgacg aacgcgacgg
     2761 ccattggtgc gaggactttg atgatcgtgg attccatatg gttatcggga actttcgctc
     2821 acgtagcggc gtgtcctcgc gacgaggtcg gagacgtcgg cgaagcgccc gttgaaggac
     2881 gagccgagga ccgcgacgac gaccgggcga ttgagaccgg cgttgaaggc gacgacgaga
     2941 ttaccgccgg cgaggtccgt gtagccggtc ttcgatgcga tgagaccgtc tatcgtcgcg
     3001 acgtcgatgt tcgtattctt cgcgatgtgg tattggccgc gcgcgttgtc tatcgtcgtg
     3061 aggtcgacgt tcgtgagcgc gaccgcgtcg gggacgttcg tgacgatgaa gtccatgagc
     3121 cgagcgacgt cgcgcgcgct cccgagcgcg ccgggttcgg tcggcgagag gtcgagaccg
     3181 gtcgggttct tgaaactggt ctgtgtaagg ccgagttctt cggcgcggac gttcatcgcc
     3241 tccacgaagg cggcgtccgg gttcgacgaa gagagcacct cgccggtacg cgcgccgagt
     3301 gccgtcgcac cgtcgttcga cgacgtgatg agcgtgaggt cggcgaggtc ctgcatggtg
     3361 aactcttcac cgtcggcgaa gccgctgtcg ccctcgacct cgatcgctct tctcgtgatg
     3421 gtgacgcggt cctccggtga aagcagttcg tacgtgagga gcgccgtcat gagcttcgtg
     3481 acggacgcca aggggagcga ttcgtccgcg ttcttcgcat agagcgcctt ctggttcttg
     3541 acgtcccaca cgatagcgcc cttcgcctca agggagacgt cgtcgaatgc gccgactcgt
     3601 gccgcttcat catgcgagcg atccgcgagc gtcgtgtcga cgcgcacttc gtcggccgcc
     3661 gcggattgct cctttaagga gtgcgaggcg ccgacgtacg tgacgccgaa aacgaagacg
     3721 aggatgccga gcgcgacgct aagctggggc acgaccggga tggtcgagac gtgcttcggg
     3781 ctttccgccg tttcatcgct aaagagctcg cttactgcgt catcgcgctc ctcatctttg
     3841 tgttcgggta tcatgatcga aatggtgaag tctcgtcgtc tcccgtcgtc tcggcctcgt
     3901 ccgcctgctt ctcgaacgcg tcgagggcgt tcatgatctc tccgaagtcg gggagggatt
     3961 cacgcctatc gataccgagg tgattgagca agtccgatgt ggcggcgtac acgaacgatc
     4021 gcgcgtcggt cgggtgcgcg cgccgctcga cgaggccgcg tatgaggagg ttcctaagga
     4081 tgaaggcgga atttacgccg cggatacggt ctatctcggc gcgtgagagc ggtccgcggt
     4141 agagcacgat ggcgagcgtc tccgcgcctg cccgccctat ctccttctcc aactcgtcct
     4201 tcctgatccc atcgatgaac tccgagagtt ccggtgcggt gacgagttgt gcctccgtgt
     4261 cggtcgtcac gagtcggacg ccgcggtcgc ggagcgactc ctgaagcgcg ccgagagcgg
     4321 tttcgatgtc ctcgagcgac acggagagaa g
//
LOCUS       DASTSP010000057         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_115831, whole genome shotgun sequence.
ACCESSION   DASTSP010000057 DASTSP010000000
VERSION     DASTSP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115831"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3428)
                     /locus_tag="VFS75_02515"
     CDS             complement(<1..>3428)
                     /locus_tag="VFS75_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677569.1"
                     /translation="VPNTQITVGANGSSTASDIVFATNVVNATGTERMRVTSNGNVGI
                     GTSTPTGLLSINPNGITGPAFVVGSSTATNFAILNTGQARFQLGSAAAPSVTFPLAVN
                     GLTTGFYGVSTDNGGIRFSGAGTDTLRLNSSGMQVYSSGVVGFGALAGTSDVAISRGA
                     AGKLYVGTGGTGNSDGTLVAGNIGIGTTSPSSKLTVAGDILGAGLTATGTVTFSGLTG
                     GVLTTNGSGVVSASTTIGTAYLDSSVLLSSEVDTSAELAALLSDETGTAGSVVFSASP
                     TLSGLLTFANASGTALTTSGNAYFGGNVGIGTTTPNNKLTVTTSGATSYLNNGTYSLV
                     LNNSLNPAASLSLQYDGSNDVGVIQTGVAGVGFIKNLALNPGGGNVGIGTTTPNVPLE
                     VLRSSGAGYTWTPNTRTTALFQSGNAAGATISIVSKSTGYSGIYFGDDSLETQGQLKF
                     DNADNHFGFTTTGGTEQLTLLTSGNLGIGTTTPNVRLSVLGSATRYNNNSTYAAELGT
                     SDNSARKLLLGYDSQIDAAIVQAVNLGVSFDKPLLLNPNNGNVGIGTTSPAYKLDVNG
                     NVMVEGTNHLSLNTSSNYFNADSSGIILNNNLIFRLNTNGSTALYANFNGNIGIGTTS
                     PSSKLTVAGDILGAGLTATGTVTFSGLTGGVLTTNGSGVVSASTTIGAGYLDSSVLLS
                     SEVDTSAELAALLSDETGTAGNAVFSTNPLLAGFRSSASSTIGDGTATGGLTISGGAT
                     TTGNTIIGGDLGLSGNSINSAYSINAAKGAYFGNTTIVGGDLSATRFLDYANSNYLLD
                     PDNTGTSLAVAGNVGIGTTTPSRMLTVTGDALFGTTTASGTLSVIDGWSSISTGHITV
                     GSGTTLATAPTLTFAAVATSGPANWTGSTKFSYNDSGFYISHDYGSGPGLAPGGFGLG
                     TGGRTDDIFIGTSGNVGIGTTSPNFKLEVGYSGTTNNTIAVSNYDTGATSYAEVLARN
                     AGAGSAQASGVRMVALGTGWTTSGGFVQNGAALDADTGLSGGLSLMARASGAAMRFYT
                     GGSADADERMRIDGNGNVGIGTTTPSSLLTVGTTSYSGSGVAGLKESYSFTNSVASAV
                     YYGNEMYIVNNPSATSTLVGKMIRIEDDSALGNTVRGLEVQTHRGTNTKGENTA"
BASE COUNT          649 a         1010 c         1081 g          688 t
ORIGIN      
        1 atcgccgtgt tctcaccctt cgtgttcgtg ccacggtgag tctggacctc gaggccgcga
       61 acggtgttcc cgagagccga gtcgtcctcg atgcgtatca tcttgccgac gagtgtcgag
      121 gtcgccgacg ggttattgac gatgtacatc tcgtttccgt aatagaccgc gctcgcgacc
      181 gagttcgtga agctgtacga ctccttgagt cccgcgacgc cagagccgga gtaagacgtc
      241 gtaccgaccg tgaggagcga gctcggcgtc gtcgtcccga tgccgacgtt gccgtttccg
      301 tcgatgcgca tgcgctcgtc cgcatcggcg gaaccccctg tgtagaaacg catcgcggcg
      361 cccgacgcgc gcgccatgag cgagagtcca cccgagaggc cggtatccgc atcgagcgcg
      421 gcgccgtttt gtacgaatcc gccgctcgtc gtccagccgg tcccgagggc gaccatacga
      481 acgcccgacg cctgcgcgga gcctgcaccg gcgtttctcg cgagcacttc cgcgtagctc
      541 gtcgcaccgg tatcatagtt cgagaccgcg atcgtgttgt tcgtcgttcc cgagtagccg
      601 acctcgagtt taaagttcgg agaggtcgtc ccgatgccga cgttgccgct cgtgccaatg
      661 aagatgtcgt cggtacgacc gcccgtgcca agaccgaatc cacccggagc gagaccaggt
      721 cccgagccgt agtcatgcga gatgtagaaa ccgctgtcgt tgtaactgaa tttcgtgctc
      781 cccgtccagt tcgccggccc gctcgtcgcg acggctgcaa atgtaagggt cggtgccgta
      841 gcgagcgtag ttcctgaacc caccgtaata tgtccggtcg agatactgct ccatccgtcg
      901 atgaccgaga gtgtaccaga cgcggtcgtc gtgccgaaga gggcatctcc cgtcaccgtg
      961 agcatgcgag agggcgtcgt cgtcccgatg ccgacgttac cagctacggc cagcgaggta
     1021 ccagtattgt cagggtcaag cagataatta ctgttggcat aatccaaaaa cctggtcgcg
     1081 gaaagatcgc cgccaacaat ggtggtattg ccgaagtaag cgcccttggc ggcattaatg
     1141 gaataggcgg aatttatgga gttgccgctt aggcctaagt ccccgccaat tatggtattc
     1201 cccgtcgtcg tcgcaccgcc actgatcgtg aggccgccgg tcgcggtccc gtcgccgatc
     1261 gtcgatgacg ctgaggagcg gaaccctgcg agtaagggat tcgtcgagaa gaccgcattc
     1321 cccgccgtgc cagtttcgtc ggagaggagg gcggcgagtt cggcggaggt atcaacctcg
     1381 gacgagagga gaaccgagga gtcgaggtat ccagcgccga tcgtcgtcga tgccgagacg
     1441 acgcccgagc cgttcgtcgt gaggacgcca ccggtgagcc ccgagaaggt gacggtaccc
     1501 gtcgcggtga ggccggcccc gagaatgtct cccgccaccg tgagcttcga ggagggcgag
     1561 gtggtgccga tgccgatgtt accgttgaaa ttagcgtaaa gtgccgtgct tccgttcgtg
     1621 ttgagacgaa agatgagatt gttattgaga atgatgccgc tcgaatccgc attgaagtag
     1681 ttcgacgatg tattgagcga gaggtgattc gtaccctcga ccatgacgtt cccgttcacg
     1741 tccaatttgt acgcggggct cgtcgtcccg atgccgacgt tgccattgtt cggattgaga
     1801 agaagaggct tatcgaagga gacaccgaga ttgaccgcct gcacgatagc cgcgtctatc
     1861 tgcgaatcgt atccgaggag aagctttcga gcgctgttgt ctgacgtacc aagctcggcc
     1921 gcgtacgtcg aattgttgtt atagcgcgtt gcgctcccta agacgcttag tctgacgttc
     1981 ggcgtcgtcg tccctatgcc gagattcccg gaggtaagaa gcgtgagttg ctccgtaccc
     2041 cctgtcgtcg tgaagccgaa gtgattgtcc gcattgtcga atttaagttg accctgtgtc
     2101 tcgagcgaat cgtcaccgaa gtagatgccg ctgtacccgg tcgacttact gacgatggag
     2161 atcgtagcac cagccgcatt accgctctgg aaaagcgccg tcgtacgcgt gttcggcgtc
     2221 catgtgtaac ccgcgccgct cgatcgaagg acttcgaggg ggacgttcgg cgtcgtcgtg
     2281 cctatgccga cgttgccacc gccggggttg agcgcgagat tcttgatgaa gccgacgcct
     2341 gcgacgccgg tctggattac accgacatcg ttgctaccgt cgtattggag cgatagtgag
     2401 gccgccgggt tgagggagtt attgaggacg agggaatatg taccgttatt gagatatgat
     2461 gtcgcacctg acgtcgtcac cgtaagtttg ttgttcggcg tcgtcgtccc gatgccgacg
     2521 ttccccccga agtacgcgtt ccccgaggtc gtgagcgccg tccccgaggc gttcgcgaac
     2581 gtaaggagac ccgagagcgt cggcgacgcg gagaacacca cgcttcccgc cgtgccagtt
     2641 tcgtcagaga ggagggcggc gagttcggcg gaggtatcaa cctcggacga gaggagaacc
     2701 gaggagtcga gatacgcggt gccgatcgtc gtcgatgccg agacgacgcc acttccgttc
     2761 gtcgtgagga cgccgccggt gagccccgag aaggtcaccg taccggtagc ggtgaggccg
     2821 gctccaagga tatctcccgc caccgtgagc ttcgaggagg gcgaggtggt gccgatgcct
     2881 atgtttccag cgacgagcgt cccatcggaa ttccccgtcc cgcccgtacc cacgtagagt
     2941 tttccagccg ccccacgaga gatggcgaca tccgatgtac cggcgagcgc accaaaaccg
     3001 acgactcccg atgagtacac ttgcattccg gaactattaa ggcgaagtgt gtccgtgccc
     3061 gcgcccgaga atcggatgcc accgttatcc gtcgacacac cgtagaatcc cgtcgtgagg
     3121 ccgttcacgg cgagcgggaa tgtgacgctc ggcgcggcgg cggaaccaag ttggaaccgt
     3181 gcctgaccgg tattgagtat cgcaaagttc gtcgcggtcg aactgccgac cacgaacgcg
     3241 ggccctgtga tgccgttcgg attgatggag agtagtccgg tcggcgtaga cgtgccgatg
     3301 ccgacgttgc cgttcgaggt gacgcgcata cgctccgtcc ccgtcgcgtt caccacgttc
     3361 gtcgcgaaga cgatgtccga cgcggtgctc gaaccgttcg caccgaccgt gatctgagtg
     3421 ttcggtac
//
LOCUS       DASTSP010000058        20218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1678276, whole genome shotgun sequence.
ACCESSION   DASTSP010000058 DASTSP010000000
VERSION     DASTSP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20218
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678276"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..972)
                     /locus_tag="VFS75_02520"
     CDS             complement(121..972)
                     /locus_tag="VFS75_02520"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU4677570.1"
                     /translation="MDTLTHAAYLWLEAHALRVALIALIAFVAHRGLGLFLERAIRGM
                     VSSRNFQSREAERKREDTLIRIGSTSAGILIWLTALLMALEEVGVAIGPLLAAAGVAG
                     LAFGFGGQYLIRDLISGMFIILENQYRIGDVVCFDATCGLVEDITLRMTTLRDLDGTV
                     HHVPHGEVKRVSNLSKDYARVNLDVRISYAADLDRVIAVVNKVGAALAEDPAWSEHIV
                     KAPQFLRVDDFGESGIVIKILGDTKPLKQWDVSGELRRRLKRALDKEKIDIPLPQLVI
                     HQGKSRG"
     gene            complement(1122..1481)
                     /locus_tag="VFS75_02525"
     CDS             complement(1122..1481)
                     /locus_tag="VFS75_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677571.1"
                     /translation="MATSNVTNVLLRVALAFAFLYPPVSAYLAPYDWIGYFPQFVHGI
                     VSDTVLLSLWGLLEVGIGLWILSGKRIALPSVIAAILLALIVLFNLAQMEVVFRDVSL
                     ALAALARAGANRRTSML"
     gene            complement(1484..2002)
                     /locus_tag="VFS75_02530"
     CDS             complement(1484..2002)
                     /locus_tag="VFS75_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677572.1"
                     /translation="MKTSIALGIVAVVIIIGGIALYSSGNTQSKTSYATPTTGTETQA
                     TAPATAGTKASNSEPGTQTGASVPKTVTVTYDGSSYSPSVVNIRTGDTVAFVDASANP
                     MWVASGVHPTHTLYDGSDTSAHCAADYAGPAPFDECGASTATYTFTFTEAGTHPYHNH
                     VNAAATGKVVVE"
     gene            complement(2146..3162)
                     /locus_tag="VFS75_02535"
     CDS             complement(2146..3162)
                     /locus_tag="VFS75_02535"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEU4677573.1"
                     /translation="MTATLLIALLLGGGTSYAAENAIPGDLLYPVKVDINENVKSAFA
                     VGDDAEAKLQADLARERLKEAEVLASRGTLDAAKSAELETRLKDHLAKADAHSQAAFE
                     KGDLEARASVESAIEGAARSYIDVLSGLNANVNGNHGDALIDELKAVADVESTSSAET
                     AVSASTDASAQVRATIDAASAAIHEMEAKLDSFGTTSTSLSARITASAKQATDAESSA
                     NALFEQGNYQAAYVRAKDAIRSATFAEVLIDSALHIRTDAKVEGKGTLEDLIGTSSPS
                     KEDEARDERSDGTNGQGKSTSTDVRHDDADIEANEHSDADVKTDLIDAHVDTDTSLKS
                     HIGL"
     gene            complement(3366..3848)
                     /locus_tag="VFS75_02540"
     CDS             complement(3366..3848)
                     /locus_tag="VFS75_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4677574.1"
                     /translation="MEQEFIETFTTYGDAIFRFSLMKVSQVELAEDMTQEVFMRYWQY
                     LRDDREITNCRALLYAIANNMAKDWYKKKKAESLDAHIVAGREPAETGMDAEALATYR
                     EVLEAAEGLDERDKEVVLLRFVEGLEPRDIAECLGETANAVSVRLNRAAQKLRMHLHI
                     "
     gene            3978..4064
                     /locus_tag="VFS75_02545"
     tRNA            3978..4064
                     /locus_tag="VFS75_02545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4012..4014,aa:Ser,seq:gga)
     gene            4250..4753
                     /locus_tag="VFS75_02550"
     CDS             4250..4753
                     /locus_tag="VFS75_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677575.1"
                     /translation="MKHVYLVFVSTFFLGFVTGGLVFLFNHTGKAGDGGIGAPAAGMS
                     ISAVAYGGCTHLGCPAYKIEDDGAYTYLTGSVSTPGRFTDSLTDRQLATLRAELADVD
                     FDAVAASRFTGTCPVAVDGVAYRFAIEYRGERHEVDTCEADVEGDPLFATLEDYFGIF
                     DLLYAGQ"
     gene            4740..5411
                     /locus_tag="VFS75_02555"
     CDS             4740..5411
                     /locus_tag="VFS75_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677576.1"
                     /translation="MRDSSTRFRLAVTAVFAALFFIFAPVAAAHVPVLIREASPSAAV
                     LIDDPELSTAFYGTLTGFPHTYEIRSSEPFLLHVEVREPDVSDARDNVSGIIVMERPE
                     GGRVEEVARLSSPAPWPSEFEFWGGDRYRVGPTFEREVRPGTYRIEVSTPDNDGARYV
                     LVMGTREELTLGYGELLGRIGDVKAFFGKSRIALVESPFAYVPVLIALSVLIFFVVRR
                     RRLIA"
     gene            5449..5859
                     /locus_tag="VFS75_02560"
     CDS             5449..5859
                     /locus_tag="VFS75_02560"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEU4677577.1"
                     /translation="MVTDLNEVAVIVAAIVSVAVGSIWYSPLLFGASWLRAARLSVEE
                     ENMTSKEASLLALKGVVVEFVFFYALALLLLASEAGGMSFAGYIGVMAALLLSFLIGI
                     TVWERKPLSYVLVNGGYTVVALAVGLSIVTFWPW"
     gene            5876..6361
                     /locus_tag="VFS75_02565"
     CDS             5876..6361
                     /locus_tag="VFS75_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU4677578.1"
                     /translation="MRWSNWTAIAVGVLLAAFLFATRSNEFSLSKLFLPTSPVMHIGD
                     VPIRVTIADTNDERIAGLSGKEGLDESEGLLFVFPSADYHAMWMKDMRFPIDIIWIGE
                     DARVISVNENVSPDSFPRTFRPARPAKYAVETNAHFVKTFGIHEGEDARLPMEYLENP
                     K"
     gene            6374..6726
                     /gene="rnpB"
                     /locus_tag="VFS75_02570"
     ncRNA           6374..6726
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFS75_02570"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            6803..9277
                     /locus_tag="VFS75_02575"
     CDS             6803..9277
                     /locus_tag="VFS75_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4677579.1"
                     /translation="MSTQGKNDGGDRVARGFVTVAENVLVVIFGLLPIIFVPIAAAPY
                     DYTKFSAVVLAVIVALVLFSLSVLRSGTLRIGVSYPLVAMWSAVAIAFISALLSGDFK
                     DALVGDFFSIHSVVFLAMLALVPTVWVLLRPGREAVIRMYLLLVVSTFTIVAFHVLRM
                     IFGPQFLSFGIFTGTVSTPLGSWNDLALFLGLIVILSLIALEQLALSRVAQWLFTIAI
                     VASLGMLAVINFFTVWLVLGLMSLVMIVYSLRRDKFEGSQLLLVEHRATSGPALILAL
                     VVFVVSVLFVIGGATIGGYIAKTVGISYVEVRPSFQATADIARNVYHENAFLGIGPNR
                     FEDAWRLYKDESINLTPFWNTDFNAGNGYISTLFVTTGVLGGLAWLTFLISFLVMGTR
                     RLLSAPASDRLWYFIGVSSFVSAVYIWGMSVIYVPGVVVLLLGSLCVGVSLVALAMLE
                     GAPARVLVVGSDRRTGFILTLVVIVVIVGSVSVLYTATRHYSSVYAFNESLLEMQNGT
                     NIDALESRVADAYALSSSDVFARRIAEFELARMNSLITVASPTDAEQKQFQDASLSGI
                     NAAQQAILIDRFEPSNWSVLGGIYGALASVNVEGAKDKAIEAYQKYVELDPKNPLPLL
                     ELAVVEGRAGDYDKAREYVNRAITLKPDFTDAFFFLSQLEIATGNVEAAIKSTQAVVS
                     LEPQNPVRYYQLGVLESFDKNPTAAISAFERAVALDPNYANARYLLALSYDEAGQSDK
                     AKAELEAVAKLNPDNAEVANLITVITEEGSLKRLRAEANKLLAEPQPTLNDTGTVSTE
                     GGTGDSSLIAPVNTAPKAETATSSGQ"
     gene            9287..10969
                     /locus_tag="VFS75_02580"
     CDS             9287..10969
                     /locus_tag="VFS75_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU4677580.1"
                     /translation="MVQRVFDIVYREVRGLHQAAYILAIFTFGSQLLALIRDRLLAHQ
                     FGAGIELDLYYTAFRIPDFLYVLFASTLSVYVLIPFIAGRMEGKGERARHLLSQIFTL
                     FLGLYALVAFLAIIAAPDVVRYFFPGFVEHTDILIPLIRLLLLQPLLLGISSLLGVIT
                     QIEHRFVLYAISPLIYNLGIILGVVVLYPIFGIEGLAFGVVLGALGHVCIQLPFILKS
                     PLRPRLTLRFDPTDMVAVFRTSLTRAVTLSLHQLVLIGLIGFASIMTVGSVSVFQFAF
                     NLQSVPLAIIGMSYSVAAFPLLAKLYAEKKHELFSENITTALRHIFFWSLPAITLFIV
                     IRAQFVRVVLGSGEFDWSDTRLTAAVLAVFVVSLVSQGMHLLLVRALYAVGNTRLPFY
                     VTLGSSTLALLFGFYFYSLFVEHGFVYRYFESLMRLDGVPGVEVLALPMGYSLALIIH
                     SIVLLVVARKALYVRLRPLVRSFYTSAVAAFFGGYVAYATLSYFGTSYEADTLMTVLS
                     EGFVAGLGGLGGVILAHYAVGSRELSEIGSALKKRFTKVDVLVPQDEDTLAV"
     gene            11015..12799
                     /gene="lepA"
                     /locus_tag="VFS75_02585"
     CDS             11015..12799
                     /gene="lepA"
                     /locus_tag="VFS75_02585"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU4677581.1"
                     /translation="MKEIRNFSIIAHIDHGKSTLADRMLELTKTVESRKMKDQVLDSM
                     ELERERGITIKMQPVRMAHTYRGTTYEINLIDTPGHIDFSYEVSRALKAVEGVLLLVD
                     ATQGVQAQTLSVLQMARDQGLTVIPVLTKIDVPHARREEVRDEVVRLLSCDPGEVRYV
                     SGRTGEGVRELLDVICERIPAPRKSERDTFRGLVFDFQYSNHRGIIVYFRVFDGSVRK
                     GERLTFLVAGRTFVALEVGIMAPEERPVSELQAGEIGYIVTGIKEPGVASVGDTLALE
                     HGSDELLEGYRAVPPVVWASVFPESQDDFTMLKQALDRLKMSDSSLSYEEESSGVLGR
                     GFRCGFLGMLHLEIVTERLKREFNIEIVITSPSIVYEVTYPDGRVDEIYAASKFPEYG
                     AKVLVREPWVLAQIILPPTYLGAVMTLLYDHEGNVIDTEVFGDGRNLLSIEMPLRELM
                     RNFFDKLKNATSGYASLSYEIAEMRSADVTRLDVLIAEDLVPAFSRVVGVRRAETEAR
                     AIVERLHGTLPRQMFVTKIQAKALGRIIASRSLSAMKKDVTGYLYGGDITRKMKLREK
                     QKRGKKKLLAHGKVNIPQEVFMKIIKSE"
     gene            13022..13369
                     /locus_tag="VFS75_02590"
     CDS             13022..13369
                     /locus_tag="VFS75_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU4677582.1"
                     /translation="MTISLMFVLAAALSVSTYDRYVAARETGAKLEEREAELAVLKER
                     AAALEAQVKHLENDRGIEEELRSRYDVVRQGEEAVILVGGERDAKARDDAGILVTKEA
                     TETPSFFERLRSW"
     gene            13433..13672
                     /locus_tag="VFS75_02595"
     CDS             13433..13672
                     /locus_tag="VFS75_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEU4677583.1"
                     /translation="MAKQVTIYSTPTCHFCHAAKSFFDEHNVAYTDFDVATDMEKRKE
                     MIDMTGQLGVPVIRVEDDVIVGFDEGKLRELLQIA"
     gene            complement(13937..14008)
                     /locus_tag="VFS75_02600"
     tRNA            complement(13937..14008)
                     /locus_tag="VFS75_02600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13973..13975),aa:Arg,seq:cct)
     gene            14121..14399
                     /locus_tag="VFS75_02605"
     CDS             14121..14399
                     /locus_tag="VFS75_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677584.1"
                     /translation="MPPENSRDHDEMLRLLRENNALLKKLYRHNIISYVLKALWYAIL
                     IGLPFAVYFYFLEPYFRAFGSDYDTFRQGMDELPGVKEIQRFLQVFVD"
     gene            14460..14532
                     /locus_tag="VFS75_02610"
     tRNA            14460..14532
                     /locus_tag="VFS75_02610"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14493..14495,aa:Phe,seq:gaa)
     gene            complement(14888..14961)
                     /locus_tag="VFS75_02615"
     tRNA            complement(14888..14961)
                     /locus_tag="VFS75_02615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14927..14929),aa:Pro,seq:cgg)
     gene            complement(15037..15537)
                     /locus_tag="VFS75_02620"
     CDS             complement(15037..15537)
                     /locus_tag="VFS75_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4677585.1"
                     /translation="MKKPSFLERLTGSIKLENEKDDGFDEHDDLESHTEDADPLRDEG
                     ADYDEDGGEEGELPIDMYQTRDAIVIRALVAGVNPDDLNISITRDMVTLKGQREEVQE
                     VPDEDYYHRELFWGSFSRSIVLPEEIVIDEAEAKEKHGLLEITLPKLDKGRSARLTVK
                     SHHPIK"
     gene            complement(15534..16229)
                     /locus_tag="VFS75_02625"
     CDS             complement(15534..16229)
                     /locus_tag="VFS75_02625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEU4677586.1"
                     /translation="MLSVQFAIAFLIGLVPALFWLWFWLREDKARPEPKSLIATAFIS
                     GMLVVALVLPLQKYAMDRFTGQNLIFVWVIIEEVLKYSAALIVVLWNKAVDEPIDAII
                     YMITIALGFSALENALFVFNPVMQGDYVNSLLTEHFRFLGATLLHVLASGTVGVSMAL
                     SYYKRSAVKIFAATAGLFIAIILHALFNFFIMDASGEAVLGVFMFVWMGIIVLFLIFE
                     KVKILEKMHRRTI"
     gene            complement(16290..18512)
                     /locus_tag="VFS75_02630"
     CDS             complement(16290..18512)
                     /locus_tag="VFS75_02630"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU4677587.1"
                     /translation="MEDTNDKKPTPQPDIPATFLKPYSPEETETKLYELWESSGYFNP
                     DVCVEKGVTAADAEPYSIVLPPPNVTGTLHMGSALMLAIEDILVRHARMSGKKTLWVP
                     GTDSAAIATQARVEKDIQKSEGKSRHELGREELLKRIDAFVEENKSTMLAQMRAIGAS
                     LDWSRYAYTMDDSRYDAVMEAFVRMYEAGLIYRGNRIVNWDPKGQTTISDDEIVYEER
                     KAKLYTFKYGKDFPISISSTRPETKVGDTAVAVHPEDPRYKEYVGKEYDVVFCDVPLH
                     IKVVADASVDKEFGTGALGVTPAHSQIDWEIADRHDLPRPQVISEYAKMTVPGRLSGM
                     KVAEAREAVVAWLREEGLLEKDEDIMQNVSTAERTGGVIEPLPKLQWWVDVDKPFTIA
                     ESKIPGIASASSLSLKELMTKVAEGGGVTFVSERFEKTYLHWAKNLRPWCISRQIWFG
                     HRIPVWYREDAMKVQVDSPGDGWEQDPDVLDTWFSSALWTMTTLGWPEETEDLKEYHP
                     TTLIETGYDILFFWVARMILMSGFFLGEVPFKTIYLHGLVRDGQGRKISKSLGNNIDP
                     VDMASKYGMDAVRISLIMGMAPGTDSRISEDKIRGYKHFANKLWNITRFVLTAVGDSD
                     LERVQGEEVLHAELSSLVMTVDRHFAEYRIDLAADAIYHFIWHRFADEIIEESKPILQ
                     SGGDAALARQKTLATILTTCLKLLHPFMPYVTEAVWQSAPTLRQGERDILMVAKWPTT
                     "
     gene            complement(18553..19725)
                     /locus_tag="VFS75_02635"
     CDS             complement(18553..19725)
                     /locus_tag="VFS75_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU4677588.1"
                     /translation="MTILSIETSCDETAVSVVQADGEFPHATYEVLGNALFSQIDIHR
                     EYGGVFPMVAKREHAKTLVPMLERALSEAGFLEATNTQLSPDVTADLHEMLYREATLG
                     DSLIGFLESHGKPPIDLIAVTSGPGLEPTLWVGINFAHALASVWNAPVVPVNHMEGHI
                     MASIFTGERIPEIRFPALALLVSGGHTELDLMRSWGHYECVGETRDDAVGEAFDKVAR
                     MFGLPYPGGPEVGARAAIARERNLPTFATLPRPMLDADNLDFSFSGLKTAVRYAIAKK
                     KLSGDDVAALARDFEDAAVEVLVEKSRRAVETYGAKTLIVGGGVSANALLRERLTSFF
                     TREYPEVTVYLPPQKLSTDNSVMIALAGHANAAHARSADEFGATKADGNLSLDAKA"
     gene            complement(19912..19984)
                     /locus_tag="VFS75_02640"
     tRNA            complement(19912..19984)
                     /locus_tag="VFS75_02640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19949..19951),aa:Val,seq:gac)
BASE COUNT         3759 a         6088 c         6029 g         4342 t
ORIGIN      
        1 cctcctcccc ctcatcgccg ccggcttcat tctcccgtac gccttgagca cgttctcgac
       61 catctacccg atgtaggtta gtgaaagcgc ccgcaaagcg ggcgctttca ctcgacggtt
      121 ctagccgcgc gacttccctt ggtggatgac gagctgcggg agcgggatat cgatcttctc
      181 cttatcgagc gcacgcttga ggcgtctacg gagttcaccc gagacgtccc actgtttgag
      241 gggcttcgtg tcgccgagta tctttatgac gatgcctgat tcaccgaagt cgtcgacgcg
      301 gaggaactgc ggcgctttca cgatgtgttc cgaccacgcg ggatcttcag cgagcgccgc
      361 gcccactttg ttgacgaccg cgataacgcg gtcgaggtcc gccgcgtacg atatgcggac
      421 gtcgagattc acgcgcgcgt agtccttcga gaggttcgac acgcgcttca cctcgccatg
      481 ggggacgtga tgcaccgtcc catcgaggtc acggagcgtc gtcatgcgga gcgttatgtc
      541 ctcgacgagc ccgcatgtcg cgtcgaaaca gacgacatcg ccgatgcggt attggttctc
      601 gagaatgatg aacatgccgc tgatgaggtc cctgatcagg tattgtccgc cgaagccgaa
      661 cgcgaggcct gcgacccccg ccgcggcgag gagggggccg atcgcgacgc cgacctcctc
      721 gagcgccatg aggagcgcgg tgagccagat gagtatgccc gcggaggtgg aaccgatacg
      781 aatgagcgta tcctctcgct ttcgttcggc ttcgcggctt tggaagtttc tcgacgacac
      841 catgcctcga atcgctcgct cgaggaagag cccaaggcct cggtgggcga cgaacgcgat
      901 gagcgcgatg agggctacgc ggagcgcgtg cgcttcgagc cagaggtagg cagcgtgggt
      961 gagcgtgtcc atagaaaagt gcgattaggt tctttcaatc tcctatctca ctaccttaca
     1021 cgcggacccc cgtggtctgt aaaggcgtct ctctgcatag agacagtaac gcgacgagga
     1081 aggcgcccgc ttcgcgggcg ccttcctcgt cataccggac gctagagcat gctcgttcgc
     1141 cgattcgccc ccgcgcgcgc gagcgcggcg agcgcgagcg agacgtcgcg gaacacgact
     1201 tccatttgtg cgaggttgaa gaggacgatg agcgcgagga gtatggccgc tatcacggag
     1261 gggagcgcga tacgcttacc cgagaggatc cagagaccga tgccgacttc gaggagtccc
     1321 cacagagaga gaaggacggt atcggatacg atgccgtgca cgaactgggg gaagtaacct
     1381 atccagtcgt acggggcaag gtatgcggat acgggcggat agagaaacgc gaaggcgagc
     1441 gcgactcgaa ggagaacgtt cgtgacgttc gatgtcgcca tactcactcg acgacgacct
     1501 tacccgtcgc cgcggcgttc acgtggttgt ggtacgggtg cgtgcccgcc tcggtgaagg
     1561 tgaacgtgta cgtcgccgtc gaggcgccgc attcgtcgaa cggagcgggg cccgcgtaat
     1621 cagccgcgca gtgcgcggag gtgtcgctcc cgtcgtagag cgtgtgcgtc gggtggactc
     1681 ccgacgcgac ccacatgggg ttcgccgagg cgtcgacgaa ggcgaccgtg tcgcctgtgc
     1741 ggatgttcac gaccgacggg ctatacgacg agccgtcgta cgtaacggtc accgtttttg
     1801 gcactgacgc gccggtctgt gtccccggtt cgctattcga tgccttcgtg ccggccgtcg
     1861 ccggtgccgt cgcttgcgtc tcagtacccg tcgtaggggt agcgtacgat gtttttgact
     1921 gcgtattacc gctcgaatag agcgcgattc cgccgatgat aatgacgacc gctacgatgc
     1981 cgagcgcgat tgatgttttc atagggtttt atgatgccac gcgaagggtt aaatttttat
     2041 gaaataaagt atagcacgct ttacatgcct cgctggggat atccctcatg gagaaacgaa
     2101 aaacgctcgc aaaatgcgag cgttttcatt ccgcactccg tcaccttaga gaccgatgtg
     2161 cgatttgagg gaggtatccg tgtctacgtg tgcatcgatg aggtcggtct tcacgtccgc
     2221 gtcggagtgt tcgttcgctt cgatatcggc gtcgtcgtgc cgcacgtccg tgctcgtcga
     2281 cttgccctgt ccgttcgtgc cgtcgcttcg ctcgtctcgc gcctcgtctt ctttagaggg
     2341 cgaggaggtt ccgatgagat cttcgagagt ccccttaccc tcgaccttcg cgtcggtgcg
     2401 aatgtggagc gcgctgtcga tcagaacctc ggcgaacgtc gcgcttcgaa tggcgtcctt
     2461 cgctcgaacg tacgccgcct ggtagttccc ctgttcgaag agcgcgttcg cgctcgattc
     2521 ggcgtcggtc gcttgcttcg cgctcgccgt aatgcgcgcg gagagggaag tagacgtcgt
     2581 cccgaacgag tcgagtttcg cctccatttc atggatggcc gctgatgccg cgtcgatggt
     2641 cgcccgaacc tgtgccgacg catcggtcga tgccgagacc gcagtctccg cgctcgacgt
     2701 cgattcgacg tcggcgacgg ccttcagttc gtcgatgagc gcgtccccat ggttgccgtt
     2761 gacgttcgcg ttgaggccgg agaggacatc gatgtacgag cgagcggcgc cctcgatggc
     2821 agactcgacg gacgcacgtg cctcgaggtc acccttctcg aaggccgcct ggctgtgcgc
     2881 atcggccttg gcgaggtggt ccttgagccg cgtctcgagt tcggctgatt tggccgcgtc
     2941 gagcgtgccg cgcgaggcga ggacctccgc ctccttgagg cgttccctcg cgaggtccgc
     3001 ttggagcttc gcttcggcgt catcgccgac cgcgaacgcg ctcttcacgt tctcgttgat
     3061 atcgaccttc acgggataga gaaggtctcc cggtatcgcg ttctccgccg cgtaagacgt
     3121 gccgccgccg aggaggagcg cgatgagtag tgttgctgtc atacgaaatg atgtgagatt
     3181 agatctgcta atgagagaag cgagtggcga cgcttcgtat atagaacgaa cgggggccgc
     3241 accacttacg tcgtggaatt gggcataccc gcggagcgct ttctcgatac gggtacggga
     3301 ggcagccgag agcttcactc cctgtgcctt ctcaagcgtc tgtcgcatgt cttgttcgtt
     3361 catattcata tatgcagatg catccgcaat ttctgtgccg ctcgattgag gcgaacggag
     3421 accgcgttcg ccgtctcgcc gaggcactcc gcgatatcgc gcggttcgag cccttcgacg
     3481 aagcggagga ggacgacctc cttgtcgcgc tcatcgagtc cctccgcggc ctcgagcact
     3541 tcgcggtacg tggcgagggc ctccgcgtcc atgccagtct cggccggttc acgaccggcg
     3601 acgatgtgtg cgtcgagcga ctccgccttc ttctttttgt accaatcctt cgccatattg
     3661 ttcgcgatgg cgtagagaag cgctcgacag ttcgtgatct ctctgtcgtc tcgaaggtac
     3721 tgccagtacc gcatgaacac ctcttgggtc atatcctcgg cgagctcgac ttgggagacc
     3781 ttcatgagag agaagcggaa gatagcgtca ccataggttg tgaacgtctc gatgaactcc
     3841 tgttccattg tcggctctgt accgttaaaa cgacccattg aggtgattct tacactgccc
     3901 gaggtctctc gcaagcgacg atgcgcgtgc gccatgggat gacgtggcgc tgattttctt
     3961 gtacagtaca ggcgcacgga ggcgtcgcat agcggtttag tgcaccacct tggaaaggtg
     4021 gcatcgggga aactcgatcg agagttcgaa tctctccgcc tccgcagaat gagaaaattc
     4081 gggattctct cgcggatttt ttcgtctctg cgcacgagat tcgaaaaggt tgtcgatgat
     4141 ttcgaccgac gggagaaatc atcatacaac ctgaactgaa gctgtaagct tcgaatctct
     4201 ccgcctccgc cctttgaact gaaaactgaa aggcggtatc gttaccgtaa tgaagcacgt
     4261 gtatctcgtc ttcgtgtcca ctttcttcct cgggttcgtg acgggagggc tcgtgttcct
     4321 cttcaaccac acggggaagg cgggagacgg cggcatcggt gcgccggcgg ccggtatgag
     4381 catctccgcg gtggcgtacg gcggctgtac gcatctcggg tgtccggcgt acaagataga
     4441 ggacgacggc gcgtacacgt acctcacggg aagcgtgtcg acgcccggcc gcttcaccga
     4501 ctcgctcacc gaccgccagc tcgcgacgct ccgcgccgag ctcgctgacg tcgacttcga
     4561 tgcggtcgcc gcgtcacgct tcacgggcac gtgtcccgtc gcggtcgacg gcgtcgcgta
     4621 tcgcttcgcg atcgagtacc gcggcgagcg ccacgaggtc gatacgtgcg aagcggacgt
     4681 cgagggcgac ccgctcttcg ccacgctcga ggattatttc ggtattttcg atctgctcta
     4741 tgcgggacag tagcacgcgt ttccgtttag ccgtcaccgc ggtgttcgcc gcgctgttct
     4801 tcatctttgc gccggtcgcg gccgcacacg ttccggtcct tatccgcgag gcatcgcctt
     4861 ccgcggcggt tttgatcgac gatcccgagc tctcgaccgc gttctatggc acgctaaccg
     4921 gtttcccgca cacgtacgag atacggagct cggagccgtt tctcctccac gtcgaggttc
     4981 gcgaaccgga cgtttcggac gcacgcgaca acgtctccgg catcatcgtc atggaacgcc
     5041 cggagggagg ccgcgtcgag gaggtcgcgc ggctctcgtc gcccgctcca tggccgagcg
     5101 agttcgagtt ctggggcggt gaccgctacc gcgtcggccc gacgttcgaa cgcgaggtcc
     5161 gtcccggaac gtaccgtatc gaggtgagta cgccggataa cgacggtgcg cggtacgtcc
     5221 tcgtcatggg gacccgcgag gagctgacgc tcgggtatgg ggaactcctc ggacgtatcg
     5281 gcgacgtgaa ggccttcttc ggaaagtcgc gcattgcgct cgtcgaatcg ccgttcgcgt
     5341 acgtcccggt cctcatcgcg ctttccgtcc tgatcttctt cgtcgtaagg agacggcggc
     5401 tcatcgcgtg agtgctatac tggtcacgtt actcgtcacc acgcatctat ggtcaccgat
     5461 ctgaatgagg tagccgtcat cgtcgccgcc atcgtgtccg tagccgtcgg aagcatctgg
     5521 tactcgccgc ttctcttcgg cgcgtcatgg ctgcgggccg cgcggctttc cgtcgaggag
     5581 gagaacatga cctcgaagga ggcgtcgctc ctcgcgctca agggcgtcgt cgtcgagttc
     5641 gtgttcttct acgcgctcgc gcttctcctt ctcgcttcgg aggcgggtgg catgtcgttc
     5701 gcggggtaca tcggcgttat ggcggcgctt ctcctctcgt ttctcatcgg tatcacggtc
     5761 tgggaacgga aaccgctctc gtacgtcctc gtgaacgggg gatacaccgt cgtcgcgctc
     5821 gccgtcggcc ttagcatcgt caccttctgg ccctggtaac atggcgaatc gtatcatgag
     5881 gtggagcaac tggaccgcca tcgccgtcgg cgtgctcctc gccgcgttcc ttttcgcgac
     5941 gcgctcgaac gaattctcac tgtcgaaact cttcctaccg acgtcgcccg tcatgcacat
     6001 cggcgacgta cccatccgcg tcaccatcgc tgacacgaac gacgaacgta tcgcgggact
     6061 ctcggggaag gaagggctcg acgagtccga gggactcttg ttcgtcttcc cgtccgccga
     6121 ctaccacgcg atgtggatga aggacatgcg ctttccgatc gacatcatct ggatagggga
     6181 ggacgcgcgg gtcatcagcg tgaacgagaa cgtgagtccc gactcgttcc cgaggacctt
     6241 tcggccggct cgcccggcga agtacgcggt cgaaacgaac gcgcacttcg ttaagacgtt
     6301 cggcatccat gaaggcgagg acgcccggct tccgatggag tatcttgaaa atccaaagta
     6361 atattgtata atacgtgggc gtctgtgatc gctcgccgaa ttggcgagag gaaagtccga
     6421 acacgcccgg cagtgccgga gtcggtagcg ggtaacgccc gtctagagcg atctacgagg
     6481 tgcgaacaga gacgccctgc gtcgaaagat aggggcatct gcgcgagcag atgagcctct
     6541 ccggtgacgg ggagggtgaa acggctaaat ccttacctgc gtgcaaggcc gagcccagaa
     6601 ccatttttgc gctagctaaa agtggtgctg ggagtgccgc atgagcggac cggtaacggt
     6661 tcgccaaggt aaatgatcac acgtccttcg ggacgacaga attcggctta tagacgtaca
     6721 aacaggcgtc ccgaaagggc gcctttttgt gtgctatcat tttttcaaca gaggtgttga
     6781 tccgattaat cttacgacca ctatgtccac ccaggggaaa aacgatggag gcgaccgagt
     6841 cgcgcgaggt ttcgtgaccg tcgcggagaa cgttctcgtc gtcatcttcg gccttctgcc
     6901 cattattttt gtgccgatcg ccgcggcgcc gtacgactac accaagttct cggcggtcgt
     6961 tctcgccgtc atcgtcgcgc tcgtcctctt tagcctctcc gtgctccgtt cgggaacgct
     7021 ccgcatcggc gtatcgtacc cgctcgtcgc catgtggagc gccgtcgcga tcgcgttcat
     7081 ctccgcgctc ctctccggag acttcaagga cgcactcgtc ggcgacttct tcagcattca
     7141 ctccgtcgtg ttcctcgcga tgctcgcgct cgttcccacg gtctgggtac tccttcgccc
     7201 aggtagggaa gcggtcattc ggatgtacct cctcctcgtc gtgagcacgt tcaccatcgt
     7261 cgccttccac gtactccgca tgatcttcgg gccacagttc ctgtccttcg gcatattcac
     7321 cggtacggtc tcgacgccct tgggaagctg gaacgacctc gcgctcttcc tcggcctcat
     7381 cgtcatcctc tcgttgatcg cgctcgaaca gctcgcgctc tctcgggtgg cacagtggct
     7441 cttcaccatc gccatcgtcg cgtctctcgg catgctcgcc gtcatcaatt tcttcaccgt
     7501 ttggctcgtc ctcggtctca tgagcctcgt catgatcgtc tactcgctcc gtcgtgacaa
     7561 attcgagggg agtcagcttc ttctcgtcga gcatcgtgcg acgagcgggc cggcgctcat
     7621 actcgcgctc gtcgttttcg tcgtctcggt cctcttcgtc atcggtggtg ccacgatagg
     7681 cggatacatc gcgaagaccg tcggtatctc gtacgtcgag gtacgtccgt ccttccaggc
     7741 gactgcggac atcgcgcgga acgtctacca cgagaacgcg ttcctcggta tcggaccgaa
     7801 ccgctttgaa gacgcgtggc ggctctacaa ggacgagtcc atcaacctca ccccgttttg
     7861 gaacaccgac ttcaacgcgg ggaacggata catctcgacg ctcttcgtca cgacgggcgt
     7921 cctcggcgga ctagcatggc ttacgttcct catatcgttc ctcgtcatgg ggacgcgccg
     7981 ccttctctcc gctcccgcga gcgaccgact ctggtacttt atcggcgtct cctcgttcgt
     8041 gagcgccgta tatatctggg gcatgtcggt catctacgtt cccggcgtcg tcgtgctcct
     8101 cctcggctcg ctctgcgtag gggtatcgct cgtcgcactc gccatgctcg agggtgcacc
     8161 cgcgcgcgtt ctcgtggtcg ggtctgaccg cagaaccggc ttcatcctca ctctcgtcgt
     8221 catcgtcgtc atcgttggct cggtgagcgt gctctacacc gcgacgcgcc actattcgtc
     8281 ggtctacgcg ttcaacgaga gcctcctcga aatgcaaaac ggcacgaaca tcgacgcgct
     8341 cgagtcgcgg gtcgccgatg cgtacgcgct ctcgtcgagc gacgtcttcg cacggcgtat
     8401 cgcagagttc gagctcgcgc gcatgaatag tctcatcacg gtcgcctcgc cgaccgacgc
     8461 cgagcagaag caattccagg acgcgagcct ctctggcatc aacgcggccc agcaggcgat
     8521 tctcatcgat cgtttcgaac cgtcgaactg gtcggttctc ggcggcatat acggcgcgct
     8581 cgcatcggtc aatgtcgagg gggcgaagga caaagcgatc gaggcgtacc aaaagtacgt
     8641 cgagctcgat ccgaagaacc cgctcccgct tctcgagctc gccgtcgtcg agggcagggc
     8701 tggtgattac gacaaggcgc gcgagtacgt gaatcgcgcc atcacgctga agcccgattt
     8761 caccgacgcg ttcttcttcc tctcgcagct cgagatcgcg acggggaacg tcgaggcggc
     8821 catcaagtcg acgcaggccg tcgtctcgct cgaaccccag aacccggtac ggtactacca
     8881 gctcggggtc ctcgagagct tcgataagaa tcccacggcg gcgataagcg ccttcgagcg
     8941 ggcagtcgcg ctcgatccga actacgcgaa cgcccgctac cttctcgcgc tctcctatga
     9001 cgaggcggga cagagcgaca aggcgaaggc cgagctcgag gcggtcgcga agctcaatcc
     9061 cgacaacgcg gaggtagcga acctcatcac cgtcatcacc gaggaggggt cattgaagcg
     9121 actgcgcgcg gaggcgaaca agctcctcgc cgagccgcag ccgaccctga acgacacggg
     9181 cacggtgagc accgagggag gtacgggcga ctcctcgctc atcgcgccgg tcaacacggc
     9241 cccgaaggcc gaaaccgcga cgtcatcggg acagtagtaa tacggcatgg tccagcgagt
     9301 gttcgacatc gtctataggg aggtacgcgg tctccaccag gccgcgtata ttcttgccat
     9361 cttcactttc gggtcgcagc tcctcgcgct catacgcgac cggctcctcg cgcatcagtt
     9421 cggcgcgggc atagaactcg acctctacta caccgcgttc cgcatccccg atttcctcta
     9481 cgtgctcttc gcgtccacgc tctccgtgta cgtgctcatt ccgttcatcg cggggcgcat
     9541 ggaggggaag ggtgaacgag cgcggcacct tctctcgcag atattcacgc tcttcctcgg
     9601 cctctacgcg ctcgtcgcgt tcctcgccat catcgccgcg ccggacgtcg tccgctattt
     9661 ctttcccggc ttcgtcgaac ataccgatat cctcatcccg ctcatacggc tcctcctcct
     9721 tcagccgctc cttctcggta tctcgagcct cctcggcgtt atcactcaga tagagcatag
     9781 attcgtcctc tatgcgataa gccccctcat ctacaacctc ggcatcatcc tcggcgtcgt
     9841 cgtcctctat ccgatcttcg gtatcgaggg tctcgccttc ggtgtcgtcc tcggcgcatt
     9901 ggggcacgtg tgcattcagc tcccgttcat tctcaagagt ccgcttcgtc cgcgtctcac
     9961 gcttcgcttc gaccccacgg acatggtcgc cgtgtttcgt acgtcgctca cgcgcgccgt
    10021 cacgctctcg ctccaccagc tcgtcctcat cggccttatc ggtttcgcga gcatcatgac
    10081 cgtcggctcc gtctcggtct ttcagttcgc gttcaacctg caatcggtac cactcgccat
    10141 catcggcatg agctactccg tcgcggcgtt cccgctcctc gcgaagctct acgccgagaa
    10201 gaagcacgag ctcttctcgg agaacatcac gaccgcgctt cggcacatct tcttctggtc
    10261 acttcccgcg atcacgctct tcatcgtcat acgcgcgcag ttcgtacgcg tcgtcctcgg
    10321 ttcgggagag ttcgactgga gcgatacgcg tctcaccgcc gcggtcctcg cggtcttcgt
    10381 ggtgtcattg gtgtcgcagg gaatgcacct tctcctcgtg cgcgcgctct atgcggtggg
    10441 gaatacgaga ctgccgttct atgtgacact cggttcttcg acgctcgcgc ttctcttcgg
    10501 gttctatttc tattcgctct tcgtcgagca cggcttcgtg tatcgttact tcgagtcgct
    10561 catgcgcctc gatggcgtcc ctggcgtcga ggtcctcgcg cttcccatgg gatattcgct
    10621 cgcgctcatc atccatagca tcgtgcttct cgtggtcgcg cggaaggctc tctatgttcg
    10681 gctccgtccg ctcgtccgct cgttctacac gtccgcggta gccgcgttct tcggaggcta
    10741 cgtcgcgtac gcgaccttga gctacttcgg aacgagctac gaggccgata cgctcatgac
    10801 ggtcctctcg gaagggttcg tcgcggggct cggcgggctc ggcggcgtca tcctcgcgca
    10861 ctacgcggtg ggaagtcgtg agctttcgga gatcggctcg gcgctcaaga agcgattcac
    10921 gaaggtcgat gtcctcgtac cgcaggacga ggacacgctc gcggtctgac ctcgcattgt
    10981 cgccgcattg tgatagagta gggccccagg gcatatgaaa gagatacgca atttttccat
    11041 aatcgcgcac atcgaccacg gaaaatcgac cctcgccgac cggatgctcg agctcacgaa
    11101 gaccgtcgag tctcggaaga tgaaagacca ggtcctcgac tcgatggagc tggagcgtga
    11161 acgcggcatc accatcaaaa tgcagcccgt tcgcatggcg cacacgtacc gcgggacgac
    11221 gtacgagata aacctcatcg acacgccggg gcatatcgac ttctcgtatg aggtgtcgcg
    11281 ggcactgaag gccgtcgagg gcgtccttct cctcgtcgac gcgacgcagg gagtccaagc
    11341 gcagacgctc tcggtcctcc aaatggcccg cgaccagggc ctcaccgtca ttccggtcct
    11401 cacgaagata gacgttccgc acgcgcggcg cgaggaggtc agggatgagg tcgtgcggct
    11461 tctctcgtgc gatcccgggg aggttcgata cgtctcggga cgaacgggcg agggcgttcg
    11521 agagcttctc gacgtcatct gcgagcgcat ccccgcgccg cgaaagtccg agcgggatac
    11581 gtttcgaggc ctcgttttcg actttcagta ctcgaatcac cgcggtatca tcgtgtactt
    11641 ccgcgtcttc gacggatcgg tacgaaaagg cgagcgcctc acgttcctcg tcgcggggcg
    11701 caccttcgtc gcgctcgaag tcggcatcat ggcgccggag gagcggccgg tatccgagct
    11761 tcaggcgggc gagatcggct acatcgtgac aggcatcaag gagccagggg tcgcgtcggt
    11821 cggcgatacg ctcgcgctcg agcatgggag tgacgagctc ctcgaagggt accgagcggt
    11881 cccgcccgtc gtctgggcct cggtgttccc ggagagccag gacgatttca cgatgctcaa
    11941 acaagcgctc gaccgcctta agatgtcgga ttcgtcgctc tcgtacgagg aggaatcctc
    12001 gggcgtcctc ggacgagggt ttcggtgcgg attcctcggc atgctccacc tcgagatcgt
    12061 gaccgagcgc ctcaagcgag agttcaacat cgagatcgtc atcacgtcgc catcgatcgt
    12121 ctacgaagtg acgtatcccg atgggcgcgt cgacgagata tacgcggcct cgaagtttcc
    12181 cgaatacggg gcaaaggtcc tcgtccgcga accgtgggtt ctcgcgcaga tcatcttgcc
    12241 gcctacgtac cttggggcgg tcatgacgct cctctacgac cacgagggaa acgtcatcga
    12301 tacggaggtc ttcggcgacg gtcgaaatct cctctcgatc gagatgccgc ttcgcgaact
    12361 catgcgaaac ttcttcgata agctcaagaa cgcgacgagc ggatacgctt cgctctcgta
    12421 cgagatagcg gagatgcggt ccgcggacgt tacgcgcctc gatgttctca tcgccgagga
    12481 cctcgttccg gcgttctcgc gcgtcgtcgg cgtccgccgc gcggagaccg aggcgcgcgc
    12541 catcgtcgag cggctccacg gaacgcttcc acgccagatg ttcgtgacga agatacaggc
    12601 gaaggcactc ggtcgcatca tcgcgtcgcg ctcgctctcc gcgatgaaga aggatgtgac
    12661 cggctacctc tatggtggcg acatcacgcg gaagatgaag cttcgcgaga agcagaagcg
    12721 cggcaagaag aagctccttg cgcacgggaa ggtgaacatc ccgcaggagg ttttcatgaa
    12781 gataatcaag agcgaatagg aaaagacccg ggaacgccgg gccttttcct attcgctcac
    12841 gtcagaacca gcgtgagtac gtgagcacca tactcagtgc gatgagtaca gcgatgaccg
    12901 accagaccca ctttatggcg gtcttcgtct ttttgtggaa taagagactc atggtgtaag
    12961 ataatagcac atgttcgatt tccacgaaaa gcggagaatg cgcggtattt tatactcacg
    13021 cgtgacgatc tcgctcatgt tcgtgctcgc agcggcgctt tccgtatcga cgtacgatcg
    13081 ctatgtcgcc gcgcgcgaga ccggcgcgaa actcgaggag cgtgaggccg agctcgcggt
    13141 gctcaaggaa cgcgccgcgg cgctcgaggc gcaggtaaag caccttgaga acgaccgcgg
    13201 catcgaggag gagctccgga gccgttacga cgtcgtgcgg cagggcgaag aggcggtcat
    13261 cctcgtcggt ggcgaacggg acgcgaaagc gagagacgac gcgggcattc ttgtgacgaa
    13321 agaagcgacg gagacgccgt cgttcttcga acggctccgc tcgtggtgag cttcattccg
    13381 gtgaaatggc agatgtggta taatcgcaat ctacgagcgt aacgcattca ctatggcaaa
    13441 acaggtaacg atatacagta cgccgacgtg tcatttctgc cacgccgcga agtcgttctt
    13501 cgatgagcat aatgtcgcgt acacggactt cgatgtggcg accgacatgg agaagcggaa
    13561 ggagatgatc gacatgaccg gccagctcgg cgtccccgtc attcgggtag aggacgacgt
    13621 catcgtcggt ttcgacgaag ggaagctccg ggaactcctc cagatagcct aagtctcaac
    13681 gcgctgaaaa aagcgagacc ttcgtctcgc tttttttgac tctcatctaa ggcgtcaggt
    13741 cgctaattcg cttcgctcat aagtgccgtc ttacagacgc tgtatacttt tcgcagaaat
    13801 tgctcaaagt cttgcgaaat gctcttcgca tttctcacct gcccggtctc gcggtttcgt
    13861 ctccttgggg tcgacgaaac atcgctccgg ctcacaaacg ctaaaagcga gacgaaagtc
    13921 tcgcttttag tgtttgtgcc cccacccgga atcgaaccag gatctcttcc ttaggacgga
    13981 atcgttctat ccattgaact acgggggcga atggatgcac ggagacgata ccacgtccga
    14041 ttccgtccta caacggaagt tctaccgcca ttcccacatc tcgcggatgg aatgcgcgcg
    14101 ccacttgcta tacttatgcc atgccacccg agaactcgcg cgatcacgac gagatgcttc
    14161 gcctccttcg agagaacaac gctctcctca agaagctcta ccgccataac atcattagtt
    14221 acgtactgaa ggcgctctgg tatgcgatac tcatcggtct tccgttcgcc gtctacttct
    14281 acttcctcga gccctacttc cgtgcgttcg gttcggatta cgatacgttc aggcagggga
    14341 tggacgagct ccccggcgtc aaggagatac aacggtttct ccaagttttc gtcgactgat
    14401 agggtctgta ctcacgccga aaatatgcta ccatcagagg gcgcaaaaat cgcgcatttg
    14461 cttgggtagc tcagttggta gagcatttgc ctgaagagca aagggtcgcc agttcgagcc
    14521 tggccccaag cacagatacg aaaaagccac cttcagggtg gtgttttcgt atctgtagcc
    14581 aggctcgaaa gccggaggcg aagccgaggc agggtagctg aaacagtatg gggtactgtt
    14641 tcagaacata ctctagggaa cgatgtcact aatgagttga aatacaaaac ccaatagaat
    14701 cagacaaata cctatcttgc ttaaatattc gtatcttctt tcccttgcac tctcttttcc
    14761 actcgtacct acgattaagt gtaaagcacc atcacgagct atctttggag gaagtccgaa
    14821 tctccaaagt aagataacgc ccgttatatc gaagagtaat cctatggcgt tgatttgact
    14881 tctcatctgg tcggggtacc cggaatcgaa ccggggtctt acgcacccga agcgcatata
    14941 ctacctctat actatacccc gaggtttggc tccgactttc ggagcttaag gactatagca
    15001 aaaaagagag tggacttcca ctctcttttc gggtaactac tttatcggat gatgactctt
    15061 tacggtgagc cgagcgcttc gtcccttgtc gagcttaggg agcgttattt cgagaagccc
    15121 gtgcttctcc tttgcctcgg cttcgtcgat gacgatctcc tcgggaagaa cgatcgagcg
    15181 ggagaaggag ccccagaaga gctcgcggtg gtagtagtct tcgtcgggaa cctcctggac
    15241 ctcttcccgc tgtcccttta gagtcaccat gtcacgggtg atcgagatgt tgaggtcatc
    15301 gggattcaca ccggcgacga gcgcgcgtat gacgatggca tcgcgcgtct ggtacatatc
    15361 gatgggcagt tcgccctcct cgcccccgtc ctcgtcgtag tcggcgccct cgtcgcggag
    15421 cggatcggcg tcttccgtgt gactctcgag gtcgtcgtgc tcgtcgaatc cgtcgtcctt
    15481 ctcgttctcg agcttgatgg aaccggtcaa tcgttctagg aatgagggtt ttttcatatg
    15541 gtacgacgat gcattttttc tagtatcttc actttttcga atatgagaaa gaggacaatg
    15601 atgcccatcc agacgaacat aaagacgccg agaacggctt caccgcttgc gtccattata
    15661 aagaagttga atagcgcgtg caatatgatg gcgatgaaca atcccgccgt cgcggcgaat
    15721 atcttcacgg cagagcgctt gtagtacgag agcgccatcg atacgccgac cgttcccgaa
    15781 gcaaggacat gaaggagcgt cgccccgagg aaacggaagt gctcggtgag aagcgaattg
    15841 acgtaatctc cctgcatgac cggattaaag acgaagagcg cgttctcgag cgccgaaaag
    15901 ccgagcgcga tggtgatcat gtagatgatg gcgtcgatgg gctcatcgac cgctttgttc
    15961 cagaggacga cgatgagcgc tgccgagtat ttgagaacct cctcgatgat gacccacacg
    16021 aaaatgaggt tctgtcccgt gaaccggtcc atcgcgtact tctggagggg aaggacgagt
    16081 gcgacgacga gcatgcccga aatgaacgcg gtcgcgatga gcgatttcgg ctccggtcgc
    16141 gccttatcct cgcggagcca gaaccagagc cagaacaggg cgggtacgag cccgatgagg
    16201 aacgcgatcg cgaattgtac ggagagcata cgcctccatt gtagcattcg aaaaacggcg
    16261 aaaccgcgtc gaaaactacc gactggggtt tacgtcgtgg gccacttcgc gaccatgagg
    16321 atgtcgcgct cgccctgccg gagcgtcggc gcgctctgcc acactgcctc tgtcacgtat
    16381 ggcatgaagg ggtggagaag tttgaggcat gtcgtgagga tggtcgcaag cgttttctgc
    16441 cgagcgagcg ccgcatcgcc gcccgattgg aggatgggtt tcgattcctc gatgatctca
    16501 tccgcgaagc ggtgccatat gaagtgatag atagcgtcag cggcgaggtc gatgcggtat
    16561 tcggcgaagt ggcggtcgac cgtcatgacg agggaactga gttccgcatg gagcacctcc
    16621 tctccctgaa cgcgctcaag gtcggagtct cccacggcgg tgagaacgaa gcgggtgatg
    16681 ttccagagtt tgttcgcgaa gtgcttatag ccgcgtatct tgtcttccga gatgcggctg
    16741 tcggtgcccg gtgccatgcc catgatgaga gagatgcgga ccgcgtccat gccgtacttc
    16801 gacgccatgt cgacggggtc gatattgttc ccgagagact tggagatctt ccgtccctgc
    16861 ccgtcgcgaa cgaggccgtg gagatagatg gtcttgaagg ggacctcccc gaggaagaag
    16921 ccgctcatga gtatcattcg cgccacccag aagaagagga tatcgtaccc ggtttcgatg
    16981 agcgtcgtcg gatggtactc cttaaggtct tcggtctcct ctggccatcc taaggtcgtc
    17041 atcgtccaga gcgcggagct aaaccacgta tcgagcacgt ccgggtcctg ttcccacccg
    17101 tctcctggcg aatcgacttg cactttcatg gcatcttcgc gataccagac cggtatgcgg
    17161 tggccgaacc atatctggcg gcttatgcac cacgggcgga ggttcttcgc ccagtggagg
    17221 tacgtcttct caaagcgttc ggagacgaac gtgacgccgc caccttccgc gactttcgtc
    17281 atgagttcct tcaatgaaag cgatgaagct gaagcgatgc cggggatttt cgattccgct
    17341 atcgtaaagg gcttgtcgac gtcgacccac cactggagct tcgggagcgg ctcgatgacg
    17401 ccgccggtgc gttccgctgt cgagacgttc tgcataatgt cctcgtcctt ctcgaggagg
    17461 ccttcctcac ggagccaggc gacgaccgcc tcgcgcgcct cggcgacctt catgccggag
    17521 agccgcccgg ggaccgtcat cttcgcgtat tcgcttatga cctgcggtcg cgggaggtcg
    17581 tgacggtcgg cgatctccca gtcgatctgg ctatgcgctg gagtgacacc gagggcgccc
    17641 gtgccgaact ccttgtcgac cgatgcgtcg gcgacgacct tgatatggag cggcacgtcg
    17701 cagaacacga cgtcgtactc cttgccgacg tattctttgt atcgggggtc ctcaggatgc
    17761 acggcgacgg ccgtgtcgcc gaccttcgtc tccggacgcg tcgatgatat tgaaatggga
    17821 aagtccttcc cgtacttaaa ggtgtagagt ttcgccttcc gctcctcgta gacgatctca
    17881 tcgtctgaga tggtcgtctg gcctttcggg tcccagttca caatgcggtt tccgcggtag
    17941 atgagacctg cctcgtacat gcggacgaac gcttccatga ccgcgtcgta ccgcgagtcg
    18001 tccatcgtgt atgcgtagcg agaccaatcg agggacgcgc cgatcgcccg catctgcgcg
    18061 agcatggtcg acttgttctc ctcgacgaac gcgtcgatac gtttaaggag ctcctcgcgg
    18121 ccgagctcgt ggcgtgattt accctcggac ttttgtatat ccttctcgac acgcgcctgg
    18181 gtcgcgatag ccgccgagtc ggtgccgggg acccagagcg ttttcttgcc gctcatacgc
    18241 gcgtggcgaa cgaggatgtc ctcgatcgcg agcatgagcg cgcttcccat gtggagcgta
    18301 ccggtcacgt tcggtggcgg aaggacgata gagtacggtt ctgcgtcggc ggccgtgacg
    18361 cctttctcga cgcacacatc cgggttgaag tagccggagg attcccaaag ctcgtagagc
    18421 ttagtctcgg tctcctcggg cgagtagggc ttcaagaagg tcgccggaat gtccggctgg
    18481 ggagtgggct tcttatcgtt cgtatcctcc atgtatcgtg atgataccag aaaatgagcc
    18541 gtttaaaagg cgctacgcct tagcgtcgag ggacaggttc ccatccgctt tcgtcgcgcc
    18601 gaactcgtcc gccgagcggg catgagcggc gttcgcgtgc ccggcaaggg ctatcatgac
    18661 gctattgtcg gtcgagagct tctgcggcgg taagtagacc gtgacctcgg ggtattctct
    18721 cgtgaagaac gacgtgagtc gctctcgaag gagcgcgttc gcggatacgc cgccgccgac
    18781 gatcaaggtc ttcgcgccgt acgtctcgac cgcgcgccgt gacttctcga cgagcacctc
    18841 gacggccgca tcctcgaaat ctcgagcgag tgccgcgacg tcgtcaccag aaagtttctt
    18901 tttagctatc gcgtagcgga ccgcggtctt gagccccgag aaggagaagt cgagattgtc
    18961 ggcgtcgagc atcgggcggg gaagcgtcgc gaaagtcggt aggtttcgtt cacgcgcgat
    19021 cgcggcgcgt gcgccgactt ccggaccgcc cgggtatggg aggccgaaca tgcgcgcgac
    19081 cttgtcgaac gcctcgccga ccgcgtcgtc gcgcgtctct cccacgcact catagtgtcc
    19141 ccacgagcgc atgaggtcga gctcggtgtg gcctcccgag acgaggagcg cgagcgcggg
    19201 gaaccgtatc tcggggatgc gctcgcccgt gaagatggac gccatgatgt gtccctccat
    19261 gtggttcacc gggacgacgg gggcgttcca taccgacgcg agcgcatgcg cgaaattgat
    19321 accgacccac agggtcggct cgaggccggg acccgaggtc accgcgatga ggtcgatcgg
    19381 aggctttccg tgcgactcga ggaaaccgat gagcgagtcg ccaagtgtcg cctcgcggta
    19441 gagcatctca tggaggtctg cggtcacatc gggcgagagc tgcgtattcg tcgcttcgag
    19501 gaaccctgct tcgctaagcg cgcgctcgag catagggacg agggtcttcg cgtgctctcg
    19561 cttcgctacc atcggaaaga cgccgccgta ctcgcggtgg atgtctatct gcgagaagag
    19621 cgcgttcccg aggacctcat aggtcgcatg ggggaactca ccgtccgcct ggacgacgga
    19681 gacggccgtc tcgtcgcatg atgtctcgat gctcaagatg gtcattcgat gcactatata
    19741 tgagttcaaa gtgattgacc attgcggggg ccggggcgtc aggtcgctaa ttcgctacgc
    19801 tcgtaagcgc ctcctgcccg gcctcgcggt tccgcctcct cgcggccggc ggaacatcgc
    19861 tccggctcgc acaaactaaa atcctgccgc agggcaggat ttttacgttt gtgcgcggtg
    19921 caggacttga acctacgacc cccacaatgt caatgtgatg ctctaccagc tgagctaacc
    19981 gcgcgggtgg tgccaatata ccagagcgcc gtaggatagg gaagtccaag cgatagtatt
    20041 gattcctatg aatgtcccgt ggtatggtgc gggcagtaca actgcgaagg aaacgccatg
    20101 gcgcacacca tagtgcaaat cggcacggta atcggcatca tcggcgtcac gctcctcgct
    20161 tccgtcctta tcgtcggctg ctgtctcgcg ctcccgtacc tcggtgagcg gctcgcga
//
LOCUS       DASTSP010000059         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1233008, whole genome shotgun sequence.
ACCESSION   DASTSP010000059 DASTSP010000000
VERSION     DASTSP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233008"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /gene="fusA"
                     /locus_tag="VFS75_02645"
     CDS             <1..2057
                     /gene="fusA"
                     /locus_tag="VFS75_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4677589.1"
                     /translation="KIGEVHDGTTVTDWMEQERERGITITAAAISCNWTPSLIADRND
                     DALKYTFNIIDTPGHIDFTAEVKRSMRVLDGAIVVFDGVAGVEPQSETNWRYADEAQV
                     PRICYINKMDRTGASFERSYDSILKRLSPKAVRFQLPWGSEENLKGVIDLLKMKAYTF
                     EGEMGMKVVEHDIPEEMKADAEKYHGELVERIVEHDDAQMSAYLEGTVPSYEELKATL
                     RKAVIANEIFPVMTGSSLKNVGVQLILDAVVDYLPSPVDLPPVKGVNPKTDAEETREA
                     SDEAPFCALAFKLQTDPFVGALTFFRVYSGTVKAGSYIYNSTTGEKERLGRIVRLMAD
                     KRSEVEEVYAGEIAAGVGLKDVKTSHTLCDEANPIILEQITFPEPVVAMRIEPKTKND
                     QEKMGMAMKKLADEDPTFKVSTDEETNETIIAGMGELHLEIIVDRMKREFGVEANVGK
                     PQVAYRETIQKPAEADYKYVKQTGGRGQYGHVKIRIRPMEKLEEGEEVAKNVTREDHY
                     EFVNAIKGGVIPNEYIPAVMKGCKEAMARGFVAGYKMEDITVELFDGSYHEVDSSEIA
                     FRLAAINAFKEAAKKAGAVLLEPIMRVEVTTPEKFMGDVNGNISSKRGLVEGMEDRGM
                     AKVIHAKVPLSEMFGYTTALRSMTEGRANMTMEFDHYEVVPPNVAAEIKKARGVKDIE
                     EE"
     gene            complement(2107..>2356)
                     /locus_tag="VFS75_02650"
     CDS             complement(2107..>2356)
                     /locus_tag="VFS75_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677590.1"
                     /translation="RKAELFDLFFLEMAKLIRVGAAIDALDGVIPTNDLIHPDYREAL
                     NPFLEALTDDELNEMLKNIMPAIVQLEVGAKKVIIEWK"
BASE COUNT          534 a          686 c          735 g          401 t
ORIGIN      
        1 acaagatcgg cgaggtgcac gacggcacca cggtgaccga ctggatggag caggagcgag
       61 agcgcggcat caccattacg gcggccgcca tctcgtgtaa ctggaccccg tccctcatcg
      121 ccgaccgaaa cgacgatgcg ctcaagtaca cgttcaacat catcgatacg cccggccaca
      181 tcgatttcac cgcggaggtg aagcgttcga tgcgcgtcct cgacggagcg atcgtcgtct
      241 tcgacggcgt cgcgggtgtc gagccgcagt ccgagacgaa ctggcgctat gctgacgaag
      301 cgcaagttcc gcgtatctgc tacatcaaca agatggaccg caccggcgcg tcgttcgagc
      361 gctcgtacga ctcgatcctc aagcgcctct cgccgaaggc cgtccgattc cagctcccct
      421 gggggagcga ggagaatctc aagggcgtca tcgatcttct caagatgaag gcgtatacgt
      481 tcgagggcga gatgggcatg aaggtcgtcg aacacgacat ccccgaggag atgaaggctg
      541 acgcggagaa gtaccacggc gagctcgtcg agcgcatcgt cgagcacgac gacgcgcaga
      601 tgagcgcgta cctcgagggc accgtgccgt cgtacgagga attgaaggcg acgcttcgaa
      661 aggcggtcat cgcgaacgag atcttcccgg tcatgaccgg ctcgtcgctt aagaacgtcg
      721 gcgtccagct cattctcgac gccgtcgtcg actaccttcc gtcgcccgtc gatcttccgc
      781 cggtcaaagg cgtgaacccg aagaccgacg ctgaggagac gcgcgaggct tccgacgagg
      841 cgccgttctg cgccctcgcg ttcaagctcc agaccgaccc gttcgtgggc gcgctcacgt
      901 tcttccgcgt ctactcgggc acggtgaagg ccggcagtta catatacaat tcgacgacgg
      961 gcgagaagga gcgcctcggc cgcatcgtcc gcctcatggc cgacaagcgt tcggaggtcg
     1021 aggaggtgta cgcgggcgag atcgccgcgg gcgtcggtct caaggacgtg aagacgtcgc
     1081 acacgctctg cgatgaggcg aacccgatca ttctcgagca gatcaccttc ccggaaccgg
     1141 tcgtcgcgat gcgcatcgaa ccgaagacga agaacgacca agagaagatg ggtatggcga
     1201 tgaagaagct cgccgacgag gacccgacct tcaaggtgtc gaccgacgag gagaccaatg
     1261 agaccatcat cgccgggatg ggtgagcttc acctcgagat catcgtcgat cgcatgaagc
     1321 gcgagttcgg cgtcgaggcg aacgtcggca agccgcaggt ggcataccgc gagaccatcc
     1381 agaagccggc cgaggccgac tacaagtacg tgaagcagac gggaggccgc ggccagtacg
     1441 gccacgtgaa gattcgcatc cgcccgatgg agaagcttga agagggcgaa gaggtcgcga
     1501 agaacgtcac ccgcgaggac cactacgagt tcgtgaacgc catcaaggga ggagtcattc
     1561 cgaacgagta catcccggcg gtcatgaagg gatgcaagga ggcgatggca cgcggtttcg
     1621 tcgccggtta caagatggag gacatcaccg tcgagctctt cgacggtagc taccacgagg
     1681 tggactcatc ggagatcgcg ttccgcctcg ccgctatcaa tgccttcaag gaggccgcga
     1741 agaaggcggg tgccgtgctc ctcgaaccga tcatgcgcgt cgaggtgacg acgccggaga
     1801 agttcatggg agacgtgaac ggcaacataa gcagcaagcg cggactcgtc gagggtatgg
     1861 aggaccgcgg tatggcgaag gtcatccacg cgaaggtccc gctctccgag atgttcggct
     1921 atacgacggc gctccgctcg atgaccgaag gccgcgcgaa catgacgatg gagttcgacc
     1981 actacgaggt cgtcccgccg aacgtcgccg ccgagatcaa gaaggcgcgt ggagtcaaag
     2041 atatagaaga ggaataacga tcacaaaaaa cggcggatcc attggtccgc cgttttcgtc
     2101 tcgagatcat ttccattcga tgataacttt cttcgcaccg acctcgagct ggacgattgc
     2161 cggcatgatg ttcttcagca tttcgttgag ctcatcgtcg gtcagcgcct caaggaatgg
     2221 attgagagcc tctctataat cgggatgaat gaggtcgtta gtgggaatga ctccatctaa
     2281 cgcatctatc gctgcaccga ctctgatcaa cttcgccatt tccagaaaga agagatcgaa
     2341 caattctgct ttgcgc
//
LOCUS       DASTSP010000060         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1457674, whole genome shotgun sequence.
ACCESSION   DASTSP010000060 DASTSP010000000
VERSION     DASTSP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457674"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VFS75_02655"
     CDS             complement(<1..788)
                     /locus_tag="VFS75_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677591.1"
                     /translation="MRTQGFLRDMNARLVFALACTAVALGVLFAVVEASSIHTYRDTL
                     SDSAPGEDATHTIEFTTASAIPPGGFVRFTPEDGAFTIPATDFDVDNVELYVDSSLRT
                     ATDTAPTATDDAVTIVTGTSGHIEVTLNSTSGIDAGSEVRLVIGNETTNSTTTDLGIE
                     NPAATGTYEYRIETGDNLGSSNEAQGLVAIIDNVGVGPVNTTETIPPVRFNGAPSGTI
                     AGTVTAVEVSLETDEFAECRYSFTPDTPFLSMTDGFDDTFTIVHS"
     gene            complement(853..1656)
                     /locus_tag="VFS75_02660"
     CDS             complement(853..1656)
                     /locus_tag="VFS75_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4677592.1"
                     /translation="MKWGRTTLVIVGALIVTALGIDAADSLGGVRGTLLSQVIGTEAK
                     GACPAGMVEVDTIGSLTCVDAYEVSAGDGCPVPNPSQLLNSQRNVNAAECHAVSKAGA
                     LPWTFISRDQAMRICAKDGKRLPTSGEWYALSLGMTDVERACNVSSGKMSESGANASC
                     VSGGVFDLVGNVWEWVSDDVTDGRYDGTALPGSGYVTAVDADGMASAIDAAPAELYEK
                     DYFWSRTDGAYGIIRGGYYDSGTDAGLYAVHADTPPGSGSIGIGFRCVK"
     gene            complement(1684..2490)
                     /locus_tag="VFS75_02665"
     CDS             complement(1684..2490)
                     /locus_tag="VFS75_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4677593.1"
                     /translation="MNGKLLKGALVVLGAVVLSTLGIFASDGIRGIDTGANIAGVGGA
                     ATCKEGSAPFKVDGNTICVDRFEESPSKGCPFQTLGSAIDTERNMASKECFAASVAGG
                     KPWTFVSLSQAERLCAALGKRLPSAKEWYDIALGTDPGGCVIHESAAKETGTASCVSS
                     ANAYDAVGNVWEWVDETVKDGTWGGRALPGEGYVTSVDENGIVITTGESADPLYGKDY
                     AWTKGEGVLGMIRGGFYGSADDAGLYTLNAAIPTSFATQGVGFRCVEDVL"
     gene            complement(2471..2875)
                     /locus_tag="VFS75_02670"
     CDS             complement(2471..2875)
                     /locus_tag="VFS75_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677594.1"
                     /translation="MAERIYRSVLRISMLVTTAALLFDGGFVFPVTKQLSDNTFLYIA
                     GAETSITATVAPNGINDLTAELTKQKKELDEREAALNARDVAARDFSSKESDYSTYIL
                     SAILFILIVLIVLNFAMDWQRARRFTYERQAS"
     gene            complement(2892..3953)
                     /locus_tag="VFS75_02675"
     CDS             complement(2892..3953)
                     /locus_tag="VFS75_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677595.1"
                     /translation="MRLVPPFPAFAILAIALIAPASASAQDSAGVTIMPAMVEKGANP
                     GDTLHETLSVTNDSAVDKDFYIFTKDIKGVEGGGMPIFADPNLEQTGYELSSWVTLPG
                     ETVHVPANDSVDVPVTVSIPTSATPGSHFGGVFVSEQPPKLRENGASVGFDVASVFSI
                     RIAGDIIDTARIREFSTDKLVYSSKHVTFKAKVENQGNILIRPRGPLSITSMFSKTPT
                     VITVNDSQAGVFPGSIRDFDFDWDDEGIGFGRYEAVLALTYDGENGQKTIDSTLVFWI
                     FPTQIVLAFIGGFLAIFLLGYFAVRYYINQAVMRASGGRRIVSSRYRRQGVSRFAFLF
                     LTFMVVVVLFLLVLLVFFA"
     gene            complement(4068..5126)
                     /locus_tag="VFS75_02680"
     CDS             complement(4068..5126)
                     /locus_tag="VFS75_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677596.1"
                     /translation="MFTKAEAANLTYVKDTLSDSDTGSVSNHTIQFLSPTGVGAGETI
                     VVTFPSEFNLATSGIAFDDIDLAENGVDKTIVAGAPAAAQWGFSTTSNTFTLTAGASE
                     SIASNATVTIKVGTNATVGATGDAQVTNPTAIGSYEFTVTAGTADSGQYRVAIVDNVL
                     VTANVNTTLTFTVSGVANGQAVNGSATNTATTTTSTSLPFETLDAGVSKTLAQDLTVA
                     TNARNGYVVTVEQDSNLQSSTGADIDGFIDGAYTDTPTAWQAPGNNVNDENTWGHWGL
                     TSDDVSLLGAGSDFGADEWVAASTTPRAIMAHNAPADGVTQNIGKARVGYQAEITALQ
                     EAGDDYSTVLTYIATPTF"
BASE COUNT          964 a         1608 c         1683 g         1012 t
ORIGIN      
        1 gagtgaacga tcgtgaacgt gtcgtcgaaa ccgtccgtca tcgataagaa gggagtatcc
       61 ggggtgaacg agtagcgaca ctcggcgaac tcgtcggttt cgagggatac ctcgaccgcg
      121 gtgaccgtac ccgcgatcgt ccccgacggc gcgccgttga agcgaaccgg gggaatggtc
      181 tccgtcgtgt tcacgggacc gacgccgacg ttatcgatga tggcgacgag accctgcgcc
      241 tcgttcgacg agccgagatt atcaccggtc tcgatgcggt attcgtacgt gccggtcgcc
      301 gcgggattct cgatgccgag gtcggtcgtc gtcgagttcg tcgtctcgtt tcctatcacg
      361 agacgaacct ccgacccggc gtcgatgccg ctcgtactat tcagtgtcac ctcgatgtgc
      421 cccgacgtgc cggtcacgat ggtgaccgcg tcgtcggtcg cggtcggcgc ggtgtccgtc
      481 gccgtccgga gcgaggaatc gacgtacagt tcgacgttat cgacgtcgaa gtcggtcgcc
      541 gggatggtga aggcgccgtc ctccggcgtg aagcggacga acccgcccgg cgggatggcg
      601 gacgcggtcg taaactcgat cgtgtgggtc gcgtcttcac ccggcgcgga atcggagaga
      661 gtgtctcggt acgtatggat ggaggacgct tcgacgaccg cgaagaggac gcccagtgcg
      721 accgcggtgc acgcgagcgc aaagacgagg cgcgcgttca tgtctcgtaa aaagccctgc
      781 gttcgcatgg tatgtactca ttgtacccca tggagagtgc gcgaggccgc tgaaaactga
      841 ataagagaga ccctatttca cgcaccgaaa gccgataccg atcgagccac tccccggagg
      901 cgtgtcggcg tggacggcgt agaggcccgc gtccgtgccg ctgtcgtagt atccgcctcg
      961 gatgatgccg tacgcaccgt ccgttcgcga ccaaaagtag tccttctcgt agagttcagc
     1021 gggcgccgca tcgatcgcgc tcgccatacc gtccgcgtcg accgccgtga cgtagcccga
     1081 gccgggaagc gccgtgccgt cgtagcgacc gtccgtcacg tcatcggaga cccactccca
     1141 tacgtttccg acgaggtcga agacacctcc cgacacgcac gaggcgttcg caccggattc
     1201 gctcatcttc cccgaggaga cgttgcacgc gcgctcgacg tcggtcatgc cgagggagag
     1261 cgcgtaccat tccccgctcg tcggtagtcg tttgccgtcc ttcgcgcaga tacgcatcgc
     1321 ctggtcgcgc gagatgaacg tccacgggag cgctccggcc ttcgagacgg catgacactc
     1381 cgcggcgttc acgttccgct gggaattcag aagctgcgag ggattcggga ccggacaacc
     1441 gtcacccgcg gacacctcgt acgcgtcgac gcacgtgagg gaacctatcg tgtcgacttc
     1501 gaccatgccc gcggggcagg cgcccttcgc ctccgtaccg atgacctgcg agaggagcgt
     1561 accgcgaacg ccgccgaggc tatcggccgc gtcgatgccg agcgcggtca cgatgagagc
     1621 gccgacgatg acgagcgtgg ttctccccca tttcatacgt tcagtatagc ggtaaccaag
     1681 gcattagagg acatcttcta cacagcgaaa gccgacgccc tgcgtcgcga acgaggtcgg
     1741 gatggcggca ttcagcgtat agagtccggc gtcgtcggcg ctcccgtaga atccgccgcg
     1801 tatcatgccg aggacgcctt ctcccttcgt ccacgcgtag tccttaccgt agagggggtc
     1861 ggcgctctcc cccgtcgtga tgacgatgcc gttctcgtcg accgaggtga cgtagccctc
     1921 cccggggagc gcgcggccgc cccatgttcc gtccttcacc gtctcgtcga cccactccca
     1981 cacgtttccg accgcatcgt acgcgttcgc tgacgatacg cacgaggcgg ttcccgtctc
     2041 cttcgcggcg ctctcgtgaa tgacgcatcc gcccgggtcg gtgccgagcg cgatgtcgta
     2101 ccactccttc gccgacggga ggcgcttccc gagagccgcg cagagacgct cggcctggga
     2161 aagggaaacg aacgtccagg gcttcccacc cgcgacggat gcggcgaagc actccttcga
     2221 ggccatattc cgttccgtgt cgatcgcact ccccaaggtc tggaacggac accccttcga
     2281 gggactctcc tcgaagcggt cgacgcagat ggtgttgccg tcgaccttga agggcgctga
     2341 accctccttg cacgtcgcgg cgccgcccac acccgcgata ttcgcacccg tgtcgatgcc
     2401 gcggatgccg tccgacgcga agatgccgag ggtcgagagg acgaccgccc cgaggacgac
     2461 gagcgctcct ttaagaagct tgccgttcat acgtgaatcg tcgcgcgcgt tgccaatcca
     2521 tcgcgaaatt gaggacgatg agcacgatga ggatgaagag tatcgcgctt agtatgtacg
     2581 tcgagtagtc gctctccttg gatgagaagt cgcgcgcggc cacgtcacga gcgttgagag
     2641 ccgcctctcg ctcatcgagc tcctttttct gcttcgtgag ttcggcggtg aggtcgttta
     2701 tgccgttcgg tgccacggtc gcggtgatgc tcgtctcggc gcccgcgatg tagaggaagg
     2761 tgttatcgga gagctgtttc gtcaccggga acacgaaccc gccgtcgaag aggagcgctg
     2821 ccgtcgtgac gagcatggag atgcggagaa cgcttctgta tatgcgctcg gccatagaaa
     2881 aagtatacac cctacgcgaa gaacacgagg aggacgagga ggaatagcac aacgaccacc
     2941 atgaacgtga ggaagaggaa cgcgaatcga gagactccct gccgtcgata gcgcgacgag
     3001 acgatgcgtc gtccaccgga ggcgcgcatg accgcctggt tgatgtagta gcggaccgcg
     3061 aagtagccga ggaggaatat cgcgaggaat ccgccgatga acgcgaggac gatctgcgtc
     3121 gggaatatcc agaagaccaa ggtcgagtcg atggtctttt ggccgttctc gccgtcgtac
     3181 gtgagcgcga ggaccgcctc gtaccgtcca aagccgatac cctcgtcgtc ccaatcgaag
     3241 tcgaagtcgc ggatggaccc cgggaagacg cccgcctggc tgtcgttcac cgtgatgacg
     3301 gtcggcgtct tcgagaacat gctcgtgatg gaaagagggc cgcgtggccg gatgaggatg
     3361 ttcccctggt tctcgacctt cgccttgaac gtgacgtgct tcgaggagta gacgagcttg
     3421 tcggtcgaga actcgcggat gcgcgccgtg tcgatgatgt cgcccgcgat gcggatgctg
     3481 aagaccgatg cgacgtcgaa gccgaccgac gcgccgttct cacggagttt cggcggctgt
     3541 tcggagacga agacgccgcc gaagtggctc cccggcgtcg ccgaggtcgg gatggagacg
     3601 gtgaccggta cgtcgaccga gtcgttcgcg gggacgtgga cggtctcccc cgggagcgtc
     3661 acccacgacg agagctcata gccggtctgt tcgaggttcg gatcggcgaa gatcggcatg
     3721 ccgcccccct cgacgccctt gatgtccttc gtgaagatgt agaagtcctt gtcgaccgcg
     3781 ctgtcgttcg tgaccgaaag cgtctcatgg agcgtgtcac cgggattcgc ccccttctcg
     3841 accattgccg gcatgatggt gacgccagcg ctatcctgcg cggatgcgga tgcgggtgcg
     3901 ataagcgcga tagcgaggat tgcgaacgcg gggaacggcg gaacgagtct catacgatgt
     3961 cgtgattgta gcacttcgct cacccgaatc gaacgtcgcg gctgtatata aacgtacgaa
     4021 agacccgcgc gatgcgcggg tcttcgtttc gtgtgtccgt aagttcgtta gaacgtcggc
     4081 gttgcgatgt aggtgagcac agtgctgtag tcatcacctg cttcctggag cgcggtgatc
     4141 tcggcctggt agccgacacg cgccttaccg atgttctgag tcactccgtc agccggcgcg
     4201 ttgtgcgcca tgatggcgcg aggcgtcgta ctcgcggcga cccactcgtc tgcgccgaag
     4261 tccgatcccg ctccgaggag cgacacgtcg tccgaggtga gaccccagtg gccccacgtg
     4321 ttctcgtcgt taacgttgtt acccggtgcc tgccatgcgg tcggcgtgtc ggtgtacgcg
     4381 ccatcgatga atccatcgat gtcagcgccc gtcgacgact ggaggttaga gtcctgctcg
     4441 acggtgacta cgtatccgtt acgagcgttc gtggcgaccg tgaggtcctg ggcgagcgtc
     4501 ttcgatacgc ccgcgtcgag ggtctcgaac gggagcgagg tcgaagtcgt cgtcgtcgcg
     4561 gtgttcgtcg cactaccgtt gactgcctgg ccgttcgcga ctcccgacac cgtgaaggtg
     4621 agggtcgtgt tgacgttcgc ggtcacgaga acgttatcga cgatagcgac acggtactga
     4681 ccagagtcgg ccgttcccgc cgtgaccgtg aactcgtacg aaccgatagc ggtcggattc
     4741 gtgacctgcg catcacccgt agcgccgacg gtcgcgttcg taccgacctt aatagtgacg
     4801 gtagcgttcg aagcgatcga ctccgaggca ccagccgtga gggtgaaggt gttcgatgtg
     4861 gtgctgaagc cccactgtgc cgccgccggc gctcctgcga cgatcgtctt atcgacgcca
     4921 ttctccgcaa ggtcgatatc gtcgaatgcg ataccgctcg ttgcgaggtt gaactcgctc
     4981 gggaaagtga cgacgatggt ctcacccgcc ccgacgcccg tcggcgagag gaactggatc
     5041 gtgtggttcg acaccgatcc ggtgtccgaa tctgagaggg tgtccttcac gtaagtgagg
     5101 ttcgcggcct cagccttcgt gaacatatga gcaccgactg cccagagcac aagcgctatt
     5161 gcaacgaagg tcgcgatccc gcgctgcata gtgacttgtc gttcataaac gtacatgtac
     5221 tgttgttttt aaacttctaa ataacttctc aagatgcctt ttgcgtg
//
LOCUS       DASTSP010000061         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1235154, whole genome shotgun sequence.
ACCESSION   DASTSP010000061 DASTSP010000000
VERSION     DASTSP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235154"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..288)
                     /locus_tag="VFS75_02685"
     CDS             complement(73..288)
                     /locus_tag="VFS75_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677597.1"
                     /translation="MNEQIPLNVDSGANKENAPARYEKTVGVKPLPGMTTAEMEAAIS
                     NPEDERARLRLLAMEEDREDIKRTYRR"
     gene            655..1854
                     /gene="tuf"
                     /locus_tag="VFS75_02690"
     CDS             655..1854
                     /gene="tuf"
                     /locus_tag="VFS75_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU4677598.1"
                     /translation="MTMAEFDRSKPHVNVGTIGHVDHGKTTLTAAILSVLNAKGDGYS
                     ASVKGVDDIDKAPEEKARGITISLSHSEYATPNRHYAHIDAPGHADYIKNMITGAAQM
                     DGAILVVAATDGAMPQTREHVLLAKQVGVPRIIVFLNKCDMVDDEEMIMLVEEELREL
                     LAKNGFDKDAPIIKGSGLKALENPAPGNEWSDKVMELVNAMDTYFPLPERDLDKPFLM
                     PIEDIFSIEGRGTVVTGRVERGILKVGDDIEIVGIKDTTATTVTGIEMFNKSLDQAQA
                     GDNAGILLRGTKKEDVHRGQVLAKKGSVTPHTEFEAEVYVLSKDEGGRHTPFFTGYKP
                     QFYVRTTDVTGEVTLAEGVEMVMPGDNAKFKVKLVAPVALEEKQNFAIREGGRTVGAG
                     VVTKIVA"
     gene            1890..2249
                     /gene="rpsJ"
                     /locus_tag="VFS75_02695"
     CDS             1890..2249
                     /gene="rpsJ"
                     /locus_tag="VFS75_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU4677599.1"
                     /translation="MTTKAKTASKKEKKESGDVQKLRIRVRAYEHKILDTSVKQIIDT
                     AMRFDAKVIGPIPLPTEIKKYTVNRSTFVFKDAREQFEMRMHKRVIDIMNPGAKVIEA
                     LTNLSLPSGVSIDVKMM"
     gene            2321..3172
                     /locus_tag="VFS75_02700"
     CDS             2321..3172
                     /locus_tag="VFS75_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU4677600.1"
                     /translation="MRTILRTYPLQETMEGAGVRLGRGIHAANAPAFDPFLMFDDFTN
                     AEEADYVPGFPMHPHRGMETVTYILGGRVRHRDSLGNEGVIEPGGVQWMTAGSGIIHE
                     EMPEVSEGGVSGFQLWVNLPRERKMTEPRYRNIVAGDIPVAKGKGHEVRVIAGTYEGV
                     AGPVSDLAVPVTYLDVSLAPDASVSLPARAGDTSFLYVVSGSVVLRDERGESWANARE
                     VALLSDGDGVELSTGATGARFLFISGLPIKEPIAWYGPIVMNTKEEIDEALWELRDGT
                     FVKTAGH"
     gene            3244..3408
                     /locus_tag="VFS75_02705"
     CDS             3244..3408
                     /locus_tag="VFS75_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677601.1"
                     /translation="MDATRRVNMQAVEAVEDDKLTGPGEFDLLEVAAGSPREGHQGGG
                     CYRTPSHIED"
     gene            3650..>4176
                     /gene="rplC"
                     /locus_tag="VFS75_02710"
     CDS             3650..>4176
                     /gene="rplC"
                     /locus_tag="VFS75_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU4677602.1"
                     /translation="MKFILGTKERMTQVFDADGVLHPVTVLRVGPAVVTQVKTKEKDG
                     YEAVQIASGAQKEHRVTKAERGHAGGAYKHMREFRPAPGRKDAMPTLEKGASIDASVF
                     AAGDVVLVSAISKGKGFQGVVKRHGFRGGPASHGMKNTLRTPGSIGATGPQRVFKGTR
                     MAGRMGGDRITVKNL"
BASE COUNT          922 a         1194 c         1292 g          768 t
ORIGIN      
        1 cgcgaattag tgagctgacg ctaagatata gcatagctaa aaaaagcgcc ggaaggcgct
       61 tttttcatag cgttaccgcc gatacgtacg cttgatgtcc tcacggtctt cctccatggc
      121 aaggaggcga agccgcgccc gttcgtcctc tgggttcgat atcgccgcct ccatctccgc
      181 cgtcgtcata ccgggaagcg gtttaacgcc gaccgtcttc tcgtaccgcg cgggtgcgtt
      241 ctccttgttc gcgcccgaat ctacattgag tggaatttgc tcgttcatag tgaaagttgc
      301 ccggtaatgg atccattgta cacccgaaat gcgaaaagga gcgctcattg cttgcaaaaa
      361 ataggtcgtt cttgtactat ggcggctacc gcattacgga aacgtaatca ggggagtttt
      421 gctcttttga catgcagtgc gggttggcag aggtaaggca agcgttctcg cgccctttct
      481 tctgaaggct cgtcacactg ctctcttttt atttttcatg aatagagagg gctgcagcgc
      541 tgagaaagtg tgggacactt tcgcagaagg ttgtcgattt ttttaaaaaa gatcatacaa
      601 cctgaacagc actggtaacg tgcggaatta ttacgaatag aattagtaac caaaattact
      661 atggcagaat ttgatcgttc gaagccgcac gtaaacgttg gtaccatcgg tcacgtcgac
      721 catggaaaga ccacgcttac cgctgcgatc ctcagtgtcc tcaacgcgaa gggcgacggc
      781 tacagcgcgt cggtcaaggg cgttgacgac atcgacaagg cgccggagga gaaggcgcga
      841 ggtattacca tctcgctctc gcactccgag tacgcgaccc cgaaccgcca ctacgcgcac
      901 atcgatgcgc cgggacacgc ggactacatt aagaacatga tcacgggtgc cgcacagatg
      961 gacggtgcta ttctcgtggt cgccgcgacg gacggcgcga tgccccagac gagggagcac
     1021 gtgcttctcg cgaagcaggt cggcgttccg cgtatcatcg tcttcctcaa caagtgcgac
     1081 atggtcgacg atgaggagat gatcatgctc gtcgaggagg aacttcgtga acttctcgcg
     1141 aagaacggat tcgacaagga cgctccgatc atcaagggtt cgggcctcaa ggcgctcgag
     1201 aacccggcac cgggtaacga gtggtccgac aaggtcatgg agctcgtgaa cgcgatggat
     1261 acgtacttcc cgcttccgga gcgtgatctc gacaagccgt tcctcatgcc gatcgaggac
     1321 atcttctcga tcgagggacg cggtacggtc gtcacgggac gcgtcgagcg cggtatcctc
     1381 aaggtaggcg acgacatcga gatcgtcggt atcaaggata cgacggcgac gacggtcacc
     1441 ggcatcgaga tgttcaacaa gtcgctcgat caggcgcagg caggcgacaa cgcgggtatt
     1501 cttctccgcg gtacgaagaa ggaggatgtg caccgaggac aggtcctcgc gaagaagggt
     1561 tcagtgaccc cgcacaccga gttcgaagcc gaggtctacg tcctctcgaa ggacgaggga
     1621 ggccgccaca cgccgttctt cacgggctac aagccgcagt tctacgtccg cacgacggac
     1681 gtgacgggcg aggttaccct cgcggagggc gtcgagatgg tcatgccggg tgacaacgcc
     1741 aagttcaagg tcaagctcgt cgcgccggtc gcgctcgagg agaagcagaa cttcgcgatc
     1801 cgcgaaggag gccgcacggt cggtgcaggc gtcgtcacga agatcgtcgc gtaagggtcc
     1861 attagcctta gggagtcatc cgaagcacca tgacaacgaa agcgaaaacc gcgtcgaaga
     1921 aggagaagaa ggagtctggc gacgtccaga agcttcggat ccgcgttcgt gcgtacgagc
     1981 acaagatcct cgacacgtca gtgaagcaga tcatcgacac ggcgatgcgt ttcgacgcga
     2041 aggtgatcgg acccattccg ctccccacgg agatcaagaa gtacacggtg aaccgttcga
     2101 cattcgtctt caaggacgcg cgagaacagt tcgaaatgcg aatgcacaaa cgtgtcatcg
     2161 acatcatgaa tccgggagcc aaggtcatcg aggctctcac gaacctctca ctcccatccg
     2221 gcgtctccat cgacgttaag atgatgtgaa gtaggtagga acgaaccgac gaaaggccgc
     2281 gcacgcgcgc ggcctttcgt cgttttggta tagtatcaag atgagaacca tccttcgcac
     2341 gtacccgcta caagagacga tggagggcgc cggcgtacgc ctcggccgcg gaattcacgc
     2401 agcgaacgct cccgcgttcg atccgttcct catgttcgac gattttacga acgccgagga
     2461 agcagactac gttcccggct tcccgatgca tcctcaccgt ggcatggaaa cggtgacgta
     2521 catcctcggt ggacgcgtgc ggcataggga cagcctcggg aacgagggcg tcatcgaacc
     2581 gggaggcgtg cagtggatga cggcggggag cggcatcatc cacgaggaga tgcccgaggt
     2641 atcggagggt ggagtctcgg gattccaact gtgggtgaat ctcccccgcg agcgaaagat
     2701 gacggagccg cgctaccgaa acatcgtggc cggcgatatc cccgtcgcga aaggcaaggg
     2761 tcacgaggtg cgcgtcatag cgggtacgta cgagggtgtc gcggggccgg tctcggacct
     2821 cgccgtgccc gtcacgtacc tcgacgtatc gctcgcgcct gatgcttccg tatcactccc
     2881 cgcgcgagcg ggggacacgt cgttcctcta cgttgtaagc ggttccgtcg ttctacggga
     2941 cgagcgcggc gagtcatggg cgaacgcgcg cgaggtcgcg ctccttagtg atggcgacgg
     3001 cgtcgaactt tcgacgggag cgacaggcgc gcgcttcctc ttcatctcgg gcctgcccat
     3061 caaagagccc atcgcgtggt acggccccat cgtcatgaac acgaaagagg agatagacga
     3121 ggcgctctgg gagctccgcg acggaacctt cgtgaaaacc gcggggcatt gacggcacag
     3181 gccgctcggg catatgattt acggagtcca ccagggaagt ggacgtccta caggagagag
     3241 agcatggacg caacaagaag agtgaacatg caggcggtcg aagccgtcga agacgacaag
     3301 ttgaccggtc cgggagaatt cgaccttctg gaggtcgccg cggggtcccc ccgtgaaggc
     3361 caccagggcg gcggctgcta ccgaaccccc tctcacatcg aggactgata cggaacgtcc
     3421 gtatacgccg ctcgacgagc gctaggtatc tcacctagcg cttttctctg cccgcttgcg
     3481 caaaacgcca cttccctata tactcacgcg agctttttaa agacctacgt acctcctacg
     3541 ggagagacca atgggcacga aaccgcggcg tctgtccgcg agcttgtgct cgcggcgacg
     3601 ccgcggtttc gtttcggtcc gcgaaagcca ttatcacatc aatctacgca tgaagttcat
     3661 actcggaacg aaggagcgca tgacgcaggt gttcgacgca gacggcgtcc tccacccggt
     3721 caccgtgctc cgcgtcggtc ctgcggtcgt cacgcaggtg aagacgaagg agaaggacgg
     3781 ctacgaggcg gtgcagatcg cctcgggcgc acaaaaggaa caccgcgtca cgaaggcgga
     3841 gcgcggccat gcgggcggcg cgtacaagca tatgcgcgag ttcaggccgg ctcccggcag
     3901 gaaggacgcg atgcctacgc tcgagaaagg tgcgtccatt gacgcgtcgg tcttcgcggc
     3961 cggagacgtc gtcctcgtgt cggcgatctc gaagggtaag ggcttccagg gcgtcgtgaa
     4021 gcgacatggc ttccgcggcg gtccggcgag ccacggcatg aagaacacgc tccgcacgcc
     4081 gggttctatc ggcgcgacgg gtccccagcg tgtcttcaag ggaacgcgga tggcgggccg
     4141 catgggcggt gaccgcatca cggtcaagaa tcttaa
//
LOCUS       DASTSP010000062         7346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1681874, whole genome shotgun sequence.
ACCESSION   DASTSP010000062 DASTSP010000000
VERSION     DASTSP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681874"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFS75_02715"
     CDS             <1..201
                     /locus_tag="VFS75_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU4677603.1"
                     /translation="MPGVEKAFAIQAGREIRVMVNPEKITDFEAHNLARSIATDIESK
                     LRYPGEIKVNVIRESRAIEYAR"
     gene            487..759
                     /locus_tag="VFS75_02720"
     CDS             487..759
                     /locus_tag="VFS75_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4677604.1"
                     /translation="MNKADIIEKIHETVGGTRADAERAVECLIDCVADSLKKGKEVSI
                     AGLGIFSTKTRNARTGRNPRTGETIDIPAMRVPKFRAAKALKDAVK"
     gene            1399..1815
                     /locus_tag="VFS75_02725"
     CDS             1399..1815
                     /locus_tag="VFS75_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677605.1"
                     /translation="MQLFAAITLLAITACVAALVTFFSVRRHFAGRVVDMSSGWMEEL
                     FPDGRYRVHIENAKKGLVSIQSFSEGTVRLCRFAEGIPEVFRKSELVTLERCTEVIEM
                     GFEYVTHVRSRRWVATPALVPTLSSEAKSEGSYATV"
     gene            complement(1852..3318)
                     /locus_tag="VFS75_02730"
     CDS             complement(1852..3318)
                     /locus_tag="VFS75_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU4677606.1"
                     /translation="MVLATVMVGTLLIGLDRTVVNLGLPGMIKDFGITVSDAAWIATA
                     YIISNAVFVPVFGKLGDKFGNRTIYLWSFVAFIVISVAAGAAWNLSSMIVLRVFQGLV
                     GAAIYPTAMALIAKTFTDPDARTQALGVWSASFAVSAVLGPLVGGPLIDNFSWRWLFY
                     VNMPVGILGLFMTLWYLPFDRPTDHKHGFDYQGAATLGVSLSALVLVLERGREWGWLS
                     AESLAAFVVAIVFGLLFYRVERRAHDPIVDLKLFSNPIFSDILILSFISFGGMMGAMF
                     LLPVFAQTYLEYNATETGLLFVPMAFTMMLSAPLGGRLSRVVPLKYLVAFGMAVSAFG
                     IFLFSGLDVRTSLFELMVPFVIFSFGLGLGMAPMTAAATNAVDPREIGVSSAVLNLVR
                     NIGGAVGIAFFGTILSNLIESNVLDIAQSTVVRSADHSVLATVRMLIVLKAQVEAYGE
                     VFFIASLVMGTGVLAAFLLKEPPKRPVLQKEADAGAFH"
     gene            complement(3361..3999)
                     /locus_tag="VFS75_02735"
     CDS             complement(3361..3999)
                     /locus_tag="VFS75_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4677607.1"
                     /translation="MNDAPLSTGTKRRRWVKSIAVMALIFGALGGYLFWRSVAGVVSI
                     DTSRLDAPIAAVAPTTAGVLNMLYVQEGDRVAANAPIAVVGSETLYAKESGIVDSAPR
                     VVGSYFGLGQPVVQIVVDEKMRVVGEIEETKGLDEIAPGKPVTFTVDAFPGRTYVGVV
                     DEIAPASNDAAVAFSISDKRPVKKFDVYMRFPVKDYPELKTGMSAKVKVYVN"
     gene            complement(4013..5104)
                     /locus_tag="VFS75_02740"
     CDS             complement(4013..5104)
                     /locus_tag="VFS75_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU4677608.1"
                     /translation="MKRSLASFFNSPALVALVSLVIAGCVAYATRDDGAEANARAYAA
                     LASRAENVVADPTGAHLTVGFTGSGRVESVKAIVGATVRTGDVLATLDPGSTAGALTQ
                     ANAAYVAARANYDKVLNGATGPAIDAAKATVHSAEVALAETKRTESLLVSQAYTALLN
                     VTPEAYPKDIQDLDQVAPTISGSYTKGVEGDIVVTTFASGAESGLSMRLSGLASGTAV
                     VSFVTPQPLGDTGLSILFPEGARAGKTWVISVPNAKASAYTTKLAAYEAAKQTERETV
                     ASAEAALAEAESNLAVVAAKARPEDVAAAQAAVDNARGALLIAESAADSRVLKAPIDG
                     TVSAVYVQEGETISADAPAFELTRTTTEQ"
     gene            complement(5107..5514)
                     /locus_tag="VFS75_02745"
     CDS             complement(5107..5514)
                     /locus_tag="VFS75_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4677609.1"
                     /translation="MKLERALPELLLSLRRKLNERMKGERFGDELTFSQLEVLSFVES
                     GPRTMERIAAHLRVKPPSVTAIVDRLEDAGLVARGKDETDRRITKVTATAKAARRLSE
                     RRERKEAAFRELLAKLSTRDRKELERILRILTS"
     gene            5698..5961
                     /locus_tag="VFS75_02750"
     CDS             5698..5961
                     /locus_tag="VFS75_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677610.1"
                     /translation="MMTSARDILVAYPDADIRSAVVGPNAADEYRGRTWENRTDKLCV
                     IISHDHDTEVFVYCDDDDALRGDLHDIPNGKHVEIYTWTLRPR"
     gene            6059..6382
                     /locus_tag="VFS75_02755"
     CDS             6059..6382
                     /locus_tag="VFS75_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677611.1"
                     /translation="MNTEDRRLQNVVTIVRMIKAVAVRVGKTGTMEGAWIAAQCDRIL
                     LAAGVTLPLEPAPVFTEDDELTLAERQARMANDYATWGGYRAEDVHAILGDPMRAVRG
                     GPRPS"
     gene            complement(6476..6970)
                     /locus_tag="VFS75_02760"
     CDS             complement(6476..6970)
                     /locus_tag="VFS75_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677612.1"
                     /translation="MLKSLVLLVLIFTGSAVAADNSMSQSVEEGPQYMRLLTREGMKD
                     GLFIVAKTHARAPKAKVAVKDYDGTMECYSWFDAILSSMWHGVEYEVSSCTDGQEFRW
                     KGPTSEGDIAWETTGLVDVLDVNGNHRGNCTLGRAYWIRETPGGWLVSPTSITRPILC
                     NLKG"
BASE COUNT         1509 a         2182 c         2238 g         1417 t
ORIGIN      
        1 atgccgggcg tcgagaaggc gttcgccatc caagcgggcc gcgagatacg cgtcatggtg
       61 aatcccgaga agataaccga cttcgaggcg cacaatctcg cgcgctcgat agcgaccgat
      121 atcgagtcga aactgcgcta tccgggcgag atcaaggtga acgtcatccg cgagtcccgc
      181 gcgatagagt acgcacgtta gtaagccatt ttttgaaaaa agggctctga tgagcccttt
      241 tttcgtggtt ttgcgctcga atcgatgctt tttgggccgc cattacaaca cgggatattg
      301 tgagtgtgaa gttattgcgc atcaaaacac gtcctatata attatttata gccatttttt
      361 ggcttcgtgc tcacgcacta gtaggacgta cacattcgga tacagatcat tggagggata
      421 tgtaccgaac gtgtaggttc tacacatcat tacaaatgcc cttaagtaca cacttcataa
      481 aatagtatga acaaggcaga tatcatcgag aagatccacg agaccgtcgg cggtactcgt
      541 gcggacgcag agcgagcggt cgagtgcctc atcgactgcg tggcggattc tctcaagaaa
      601 ggtaaggagg tgtcgatcgc tggactcggc atcttctcga cgaagacgcg aaacgctcgc
      661 actggccgca atccgcggac cggcgagacc atcgatatcc ctgctatgcg cgtaccgaag
      721 ttccgcgcgg caaaggcact taaggacgca gtgaaatagt cactccttcc gaaaaggccg
      781 cgactccgtc gcggcctttt cattttcgct tgcattcctc gtcatcatgg tataatggaa
      841 gcgtaccgga ggtattaact cggtccacat gtacaccgag ttgacggtat agagtcatag
      901 aacttagttc tatctctcac cacagcaaaa gccccgcgcg aggggctttt gcgttgaatt
      961 gatctcccag atcaatccag agcgaaaggt gtaccgtccc tgtaggggaa ttttgcgttc
     1021 tcttcacttc ttttcctcga tttggtagta tctgtgcggc acgaaaacgg gaaaggagta
     1081 aacctatgtc tgcggcgctc gaaccggacc cgcgagatac gggggttctc tgtccgaaat
     1141 gcgcgaagga gcgagaccag ccttcaaaga tattcaaaac cggaagcggc taccagtgcc
     1201 agcatccgca ctgcctcaag ttcttcttgg aactgccgct taaagcggcg gcgcatcgcg
     1261 agttgcacag agagtgaggc actatgacga ccgccgagca agttcggcgg ttgttttttg
     1321 ttcgaaaaga gcgtataacg ggcgctggtc agttccactc atggaggaaa catggaagcg
     1381 acgtgcacgc tacaaggaat gcagctcttc gccgcgatca cgcttcttgc cataaccgcg
     1441 tgcgtggccg cgctcgtcac attcttctcg gtccgccgcc acttcgcagg gcgagtcgtc
     1501 gacatgtcga gcgggtggat ggaagagctc tttccagacg ggcgatatag ggtccacatc
     1561 gagaacgcta agaaggggct cgtttccatc caatcgttct ccgaaggcac cgtacgcctc
     1621 tgcaggttcg ccgagggaat ccccgaggtc ttccgcaaga gcgagcttgt aacgctcgag
     1681 aggtgcacag aagtgatcga gatgggcttt gaatatgtca cacatgtgcg ttcgcgccgt
     1741 tgggtggcca cgccagccct cgttccgact ctctcatcgg aggcgaagag cgaaggatcg
     1801 tacgccaccg tctgagagcg taaaaatcgc cgagtaatac tcggcgattt tttaatggaa
     1861 cgcacccgcg tccgcctctt tctggagcac aggccgcttc ggcggctcct tgaggagaaa
     1921 cgccgcgagg acgccggtcc ccatgacaag ggacgcgatg aagaagacct cgccgtacgc
     1981 ctcgacctgc gccttaagga cgatgagcat gcgcacggtc gcgaggaccg agtgatccgc
     2041 gcttcgcacc acggtacttt gcgcgatgtc gagtacgttc gattcgatga ggttcgagag
     2101 gatggtgccg aagaacgcga tgccgaccgc gccgccgata ttgcggacga ggttgaggac
     2161 ggcggacgag acgccgatct ctcgcgggtc gaccgcgttc gtcgccgccg ccgtcatggg
     2221 cgccatgccg agcccgagac cgaacgagaa gatgacgaag ggcaccatga gctcgaaaag
     2281 ggacgtccgt acgtcgagac ccgagaagag gaatatgccg aacgcggaga ccgccatgcc
     2341 gaaggcgacg agatacttaa gcgggacgac ccgcgaaagt cgcccaccca ggggagccga
     2401 gagcatcatg gtgaacgcca tcggcacgaa gagaagcccc gtctctgtcg cgttgtactc
     2461 gaggtaggtc tgcgcgaaga ccgggagaag aaacatggca cccatcatgc cgccgaacga
     2521 aatgaaggag agtatgagga tgtccgagaa gatggggttc gaaaaaagct tcagatcgac
     2581 gatgggatca tgcgcgcggc gctcaacacg gtagaaaagg aggccgaaga cgatggcgac
     2641 gacgaacgcg gcgaggctct ccgcggagag ccagccccac tcgcgtcctc gctcgaggac
     2701 gagaacgagc gcagagagcg acacgcccaa tgtcgccgct ccctggtagt cgaagccgtg
     2761 cttatggtcc gtcgggcggt cgaagggcaa ataccacagc gtcatgaaaa gaccgaggat
     2821 gccgacgggc atgttcacgt agaaaagcca gcgccacgag aagttgtcga tgagcggtcc
     2881 gccgacgagg ggtccgagga ccgcggagac cgcgaacgag gcggaccaga cgccgagagc
     2941 ctgcgtgcgc gcgtcggggt cggtgaaggt cttcgcgatg agggccatcg cggtcgggta
     3001 gatcgccgcg ccgacgagtc cctggaaaac gcggagaacg atcatcgagg agaggttcca
     3061 cgcggcaccg gcggctaccg aaatgacgat gaacgcgacg aagctccaca ggtagatagt
     3121 acggttgccg aacttgtcgc cgagtttccc gaagacggga acgaagaccg cgttcgagat
     3181 gatgtacgcg gtcgcgatcc aggccgcatc cgacaccgta atgccgaagt ccttgatcat
     3241 gccggggagc ccgaggttca cgaccgttcg atcgagcccg atgaggagcg tgccgaccat
     3301 gacggtcgca aggaccatcc agggcgatcc cccttccttt gtaggggatg cgttcatgcg
     3361 ttagttcacg tacaccttca ccttcgctga cataccggtc ttcagctccg gatagtcttt
     3421 gaccgggaat cgcatgtaca cgtcgaactt cttcaccggg cgcttatcgg agatggagaa
     3481 cgcgaccgcg gcgtcgttcg aggcgggcgc tatctcatcg acgacgccga cgtacgtgcg
     3541 gccgggaaag gcgtcgaccg tgaaggtcac cggtttcccg ggcgctatct catcgagccc
     3601 cttcgtctct tcgatctcac cgacgacgcg catcttctcg tcgacgacta tctgcacgac
     3661 cggctggccg agcccgaagt acgagccgac gacgcgcggc gccgagtcga cgataccgga
     3721 ctccttcgca tagagcgtct ccgagccgac gaccgcgatg ggagcgttcg ccgcgacgcg
     3781 gtcgccctcc tggacgtaga gcatgttgag aacgcccgcc gtcgtcggcg cgaccgcggc
     3841 gatcggcgca tcgaggcgcg acgtgtcgat cgacaccacg ccggcgaccg accgccaaaa
     3901 gaggtagccg ccgagcgcgc cgaagatgag cgccatgacg gcgatgctct tcacccagcg
     3961 tcgccgtttc gttcccgtac taagaggtgc gtcgttcatg tgatgaatat gattactgtt
     4021 cggtagtcgt tctcgtaagt tcgaacgcgg gtgcgtccgc tgagatcgtc tccccttcct
     4081 ggacgtacac ggcgctcacc gtgccgtcga tcggtgcctt gagaactcga ctatcggcgg
     4141 ccgattctgc gatgagaagc gcgccacgcg cgttatcgac cgccgcctgc gccgccgcga
     4201 cgtcctcggg gcgagccttc gcggcgacga ccgcgagatt cgactccgcc tccgcgagcg
     4261 ccgcttccgc cgatgcgacc gtttcgcgct cggtctgctt cgcggcctcg tacgcggcga
     4321 gcttcgtcgt ataggcgctc gccttcgcgt tcggaacgga gatgacccat gtcttccccg
     4381 cgcgagcgcc ctccgggaag agaatggaga ggccggtgtc accgagcggc tgcggggtca
     4441 cgaacgagac gaccgcggtc ccggacgcca gcccggaaag ccgcatagag agtcccgact
     4501 ccgcgccaga cgcgaacgtc gtcacgacga tgtcgccctc gacgcccttc gtgtaactcc
     4561 ccgagatggt cggcgcgacc tggtcgaggt cctgtatatc cttcggatac gcctcgggcg
     4621 taacgttaag gagggcggtg tacgcctgag agacgaggag gctctcggtt cgcttcgtct
     4681 ccgcgagagc gacctcggcg ctatgaacgg tcgccttcgc cgcgtcgatg gccggaccag
     4741 tggcaccgtt cagcacttta tcgtagttag cccgcgccgc tacgtacgcg gcgttcgcct
     4801 gcgtgagggc gcccgccgtc gaacccgggt cgagcgtcgc gaggacgtcg cccgttcgaa
     4861 cggtcgcgcc gacgatcgcc ttcaccgact cgacgcgtcc gcttcccgtg aagccgacgg
     4921 taaggtgtgc tcccgtcgga tcggcgacga cgttctcggc gcgcgaagcg agcgccgcgt
     4981 acgcccgcgc attcgcctcg gcaccgtcgt cgcgcgtcgc gtacgcgaca cacccggcga
     5041 tgacgagcga gactaaggcg acgagcgccg gactattgaa gaatgatgcg agtgatcgtt
     5101 tcatggttat gaggtgagta tgcggagtat tcgctcgagc tcctttctgt ctcgcgtgga
     5161 gagcttcgcg aggagctctc gaaacgcggc ttccttccgt tctctccgct cagagagtcg
     5221 tcgtgcggcc ttcgccgtcg ccgtcacctt cgtgatgcgc cggtccgttt cgtccttccc
     5281 tcgcgcgacg agtccggcat cttcgaggcg gtcgacgatc gcggtgaccg acggcggttt
     5341 cacacggagg tgcgccgcga tgcgctccat cgtgcgaggt ccgctctcga cgaacgagag
     5401 cacctcgagt tgcgagaacg tgagctcgtc accgaagcgc tcccctttca tgcgttcatt
     5461 gagcttccgt cggagcgaaa ggaggagctc cgggagcgcg cgttcgagtt tcattagctt
     5521 tgctaatagt taggtaatct aatataaacg accggcgcgt cacggtcaat cccctattgc
     5581 ctgcttgcct acgccgtcgt ataacggaac gagttctaac gacgagagga ggccgctgtg
     5641 aagcgtgtac tgctcggtac attgtccttt tgcatcgcga ccatagcgtc tgcgacgatg
     5701 atgacctcgg cgcgcgatat cctggtcgca taccctgacg ccgacatcag gagcgcggtc
     5761 gttggcccga atgctgccga cgagtaccgc ggccggacat gggagaaccg tacggacaaa
     5821 ctgtgcgtca ttatctccca cgaccacgac accgaggtct tcgtctactg cgatgacgat
     5881 gacgcgctcc gcggagatct ccacgatatt ccgaacggaa agcacgtcga gatatatacg
     5941 tggacactgc gaccacggtg agatcgcact agccgcgagc acatgctcgc ggttgttttt
     6001 tctcccgacg ggccgtataa caaaagaagg accgttccat ttcaaaggga gagataccgt
     6061 gaacacggaa gataggcggt tgcagaatgt cgtgaccatc gtgcggatga tcaaggcggt
     6121 cgccgtgcgc gtcgggaaga ccggcaccat ggagggcgcg tggatcgcgg cgcaatgcga
     6181 ccgcatcctc ctcgctgccg gcgtgacgct ccccctcgag cccgcgcccg tcttcaccga
     6241 ggacgacgag ctcacgctcg ccgagcggca ggcgcgcatg gcgaacgact acgcgacgtg
     6301 gggcggctat cgggccgagg acgtccatgc catcctcggt gacccgatgc gggcggtgcg
     6361 cggtggcccg cgtccgtcgt aagtgattcg atgctcctaa agagagcccg ccagacatcg
     6421 tctggcggtt ttttatataa agaaacccca cactcgaaag tgtgggggtg ctccgtcagc
     6481 ccttgagatt gcagaggatc ggtcgcgtta tcgacgtcgg cgagacgagc cagccacccg
     6541 gtgtctcgcg tatccaatac gcgcgcccga gggtgcagtt gccacggtga ttcccgttca
     6601 cgtcgagcac gtcgaccaga ccagtggtct cccaggcgat gtctccttcg ctcgtcggac
     6661 ctttccagcg gaattcctgt ccatcggtgc aactcgacac ctcgtactcg acgccgtgcc
     6721 acatcgagga gaggatggcg tcgaaccacg agtagcactc catggtgccg tcgtagtcct
     6781 tgacggcgac cttcgccttc ggcgctcgcg cgtgcgtctt cgcgacgatg aagaggccgt
     6841 ccttcatgcc ctcgcgggtg aggagccgca tgtactgcgg tccctcctcg accgactggg
     6901 acatcgagtt gtccgcggcg accgcgcttc cggtgaaaat gagcacgagc agtaccagcg
     6961 atttcagcat ctctctctcc tgtgtgtggg ggcgatacta attaaacgct acaatagtat
     7021 ataccaattg tcaatatccg acccgtttca agaggaatcg gttcttccac ggcattcgcc
     7081 gctttgggga aagaggaatg gaagcttaca gcttcagtac acgttgtcgc tgtttatgag
     7141 gaatcgtccc gctttttcta attcgctgcg ctctgcgggc aagaggaatc gaaacttaca
     7201 gcttcagtac aggttgtcgc tattttaggt cgcttctctc ctaaaatagc aggcaacctc
     7261 ttcgtttcct cacgtacagt gccccgcact gtactttgct ccgcacagat gcaacaaaaa
     7321 accgcctcga aagaggcggt tttttg
//
LOCUS       DASTSP010000063        10789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1237739, whole genome shotgun sequence.
ACCESSION   DASTSP010000063 DASTSP010000000
VERSION     DASTSP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237739"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1074
                     /gene="nusB"
                     /locus_tag="VFS75_02765"
     CDS             172..1074
                     /gene="nusB"
                     /locus_tag="VFS75_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU4677613.1"
                     /translation="MSNRHLSRSIVLQSLFEWDLNNIEKEHVKEALERNVEEFAPHKA
                     DMPFMERLLDGVLQKQKELDLVIEKAAPEWPIERIAPVDRNILRLGLFELLFADRKEV
                     PAKVAINEAIELAKQFGGENSSRFVNGVLGAVYKEIGEPGKEEVSRNKRRKDIPFEEM
                     EIERLAGAVVYAVHEGTMYLALVHDIFGHWTLSKGKLEANEEVKEGAKRSLKDEIGLD
                     VEIEAELGKNEYVASHPEKGRIRKQVTYFLAKSEYVNLTLEQKGGLDDAQWFKVADIL
                     ELNFYEDILPIVTKAVTMLVSKRT"
     gene            1125..1838
                     /gene="rnc"
                     /locus_tag="VFS75_02770"
     CDS             1125..1838
                     /gene="rnc"
                     /locus_tag="VFS75_02770"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU4677614.1"
                     /translation="MTEPDKIPESLERLETIIGVVFRDRTHLLTAITHRSYLNEHREA
                     TQEHNERYEFLGDAVLELIVTDFLFRKYPARPEGELTAIRAALVNTTSLAEAATNLAI
                     NDFLLLSKGEEKDTGRARQYILANTFEAIIGAIYLDMGYDAALDFVSRQLFGKTDVVV
                     EKQLWRDAKSRFQELAQEHESITPSYETLSQSGPDHDRVFTVGVFLGGAKVAEGKGRS
                     KQEAEQLAAEAGIRAKRWD"
     gene            1904..2389
                     /locus_tag="VFS75_02775"
     CDS             1904..2389
                     /locus_tag="VFS75_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4677615.1"
                     /translation="MRAKTGGWTMAEMIIVIAVIALLASVVIPNLVGSKGHARDTSRI
                     SDLKQIQLAFRTWRDDYSTSTYPVYASGDVIGDGVGVETVFAPYVGGTIKDPIDSGSN
                     RYYYDSDYTCNGASHVIVMALTMEEATSGNYESVCGAGSEGDLGNGVTPTAASYVVIL
                     K"
     gene            2392..4683
                     /locus_tag="VFS75_02780"
     CDS             2392..4683
                     /locus_tag="VFS75_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4677616.1"
                     /translation="MYLKELTINGFKSFAKKSDLEFTTPITAIVGPNGSGKSNVAEAF
                     RFVLGEQSVKSMRGKKGEDLIWGGSESVSRSNRASVTIKLNNPSRNGSRVFPLDFDEI
                     TIERVVNRDGTNEYSINGSKTRLKDVQELLAAANIGPTGHHIISQGEADRILSANPKE
                     RREMIEDALGLKVFQFKKAEAERKLKKTRENIAQVESLRREVAPHLRFLEKQIKKIEK
                     SLALQEELKTVYAEYLKREDTYIAFHTDRLTEERRAPAARREELKVLVAAAKEKLQAR
                     EKDDTADALVALEEKMSAARAKRQELVRTMGQLEGQILFLERQAAQREKRRESAAEEP
                     VPRSEVKRVFDDAERAASSAGEDAKALHRALHDTVASLKMLLTHAFHDEKDAEERASA
                     EELSALKETKSESERDVAAVEKEEAELSREYAELKRKVESENVESREAEREIFALMNE
                     EREVEQKLDAIDRELSVLVRDRDEFKNELQEAVSLIGRSASMYFEYEVKGEDGAALPA
                     AAMTAEERSVQVGRRRELERMKIRLEELGTGANEDVVKEYKEVQERDEFLVHEITDLT
                     ESVAKLEALIEELTNDLNEQFLSGIEKIGEEFRKFFTLMFGGGSAELLRVKPKPKKRE
                     EGDEEADAALADEGEEVSEESEEGIEVDVKLPNKRVRGLDMLSGGERALTSIALIFAM
                     SQVNPPLFVILDETDAALDEANSRRYGDMISALAEKSQLILITHNRETMGRAGVLYGV
                     TMAGDGVSKLLSVKFEEAVAVAK"
     gene            5187..5336
                     /locus_tag="VFS75_02785"
     CDS             5187..5336
                     /locus_tag="VFS75_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677617.1"
                     /translation="MPKCTEDEQFPCQTCAACELRAKLRPFLDWSGRQPIHTDKARLG
                     NFGPH"
     gene            5474..5683
                     /locus_tag="VFS75_02790"
     CDS             5474..5683
                     /locus_tag="VFS75_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677618.1"
                     /translation="MAKNKCSLCSGAFKVFAPENCPACGGGGASARVAFIRPKATPGK
                     GGAAKRLPPEVLANVRSSEVEHAFA"
     gene            complement(5756..7699)
                     /gene="rnr"
                     /locus_tag="VFS75_02795"
     CDS             complement(5756..7699)
                     /gene="rnr"
                     /locus_tag="VFS75_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU4677619.1"
                     /translation="MDTKKATYEGAIMIRGKGTGFVTVPGHDADILIAPENLGFALDG
                     DIVEIEIQKLVPGKREEGKVLRVIDVKRREFIGTVVKKDGGYKLQPDNRRIHVRLALP
                     DATKQDEGMKVVAEIKEWKNPLSEPIAEITEVIGRAGDHETEMQAIIRSGGFSENFPP
                     HVAEAGKQLYARRDEIFKEAEEATKGPNPRRRDMRDRTTMTIDPVDAKDFDDAISFHK
                     LENGNFEIGIHIADVSHYVREEDTIDKEAQSRGTSIYLVDRVIPMLPEVLSNDLCSLR
                     PNEDRLAFSAVFEMNKDGAILGEWFGQTLIHSNKRFSYEEAQEVLDTNEGPLKEELHE
                     LMAIGRKLRKERYEEGAIAFDQPEVRFELDENGKPIHVYAKVRTETMLMIEDYMLLAN
                     RRVAEFVYNHTKKKGRDAAFVYRVHDVPNPEKIENLALFLHALGYEFKTHKGSVDAKD
                     INKLLKSIEGTPEENLIKTATIRSMAKAVYTTKNIGHFGLAFKFYTHFTSPIRRYPDL
                     MAHRLLRKHLDGSPIGGREIAKYESLSIQSSERELTAVTAERDSIKFKQVEYMQDKVG
                     QVFDAVVTGVADWGIYVEDKVSRSEGMIRLSSMKNDYFTHEATKYRVKGNRTGKTYSL
                     GDEVKVKLVRADMEERVLDFELA"
     gene            7828..8319
                     /gene="rpsP"
                     /locus_tag="VFS75_02800"
     CDS             7828..8319
                     /gene="rpsP"
                     /locus_tag="VFS75_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU4677620.1"
                     /translation="MRLQRIGRKNQPEYRVIVTDSRNAAKRGMSVDTLGSYQPKLGVF
                     KIDGDRAKDWISKGVQVSDTVHNFLVSEKIIEGKKKNVLPKKSPIIDEEKLKAEAEAK
                     AAAEAKAAEEAAAAAAPVEEAPAETASEVPTETPVSEASAEAAPAEPAAPAEEKAPEE
                     KAE"
     gene            8469..8594
                     /locus_tag="VFS75_02805"
     CDS             8469..8594
                     /locus_tag="VFS75_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677621.1"
                     /translation="MDSEVNASPMAAEPEEQATPESTEETTTEAPAPMPEEPAAE"
     gene            8759..9109
                     /locus_tag="VFS75_02810"
     CDS             8759..9109
                     /locus_tag="VFS75_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU4677622.1"
                     /translation="MESDQQFLETLVKALVDHPESVKINRTVDEMGVLLTLDVHAEDM
                     GKIIGRSGNTAKAIRTLLRVVGMKNEARVNLKINEPEGGTRSAQTDMASDANTDETPA
                     GPKSLDEAIDGLRI"
     gene            9292..9726
                     /locus_tag="VFS75_02815"
     CDS             9292..9726
                     /locus_tag="VFS75_02815"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4677623.1"
                     /translation="MTKGIDYTGISVVALCHDGKGKYLLELRSEKCRDERLRWSNVGG
                     GGLEAHETLEEAVRREIKEECGADVLVIEPLGFREVFRESDDGTKSHWIAFDFKVEID
                     PAQVSVREPEMCLEHRWVALSEFPEPLHSQFPHFLEKYRDRL"
     gene            9729..10400
                     /gene="trmD"
                     /locus_tag="VFS75_02820"
     CDS             9729..10400
                     /gene="trmD"
                     /locus_tag="VFS75_02820"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU4677624.1"
                     /translation="MRFHVITIFPEVCKAYTDASVLGRAQKTEKGKGAKHRGKKLEVR
                     YVNPRDFAKNKHKKVDDRPYGGGPGMVMYAEPIIRAWEKAVGKRKGKVKTLIMSPRGK
                     TFTAKMAKDLAEKFDDVVLISGRYEGIDARVKKVLKAEEVSIGDYVLTGGELPALTVI
                     DAVARHIPGVLGTFESLEDERISSGVMYTRPEVLIHKGKKYKVPPVLLRGNHAEIESW
                     RKTAK"
BASE COUNT         2339 a         3043 c         3381 g         2026 t
ORIGIN      
        1 atcaaggcga aggaggagat catcaagaac gaggcggtct cgtcgagcgc gcccgccgac
       61 gacaaagagg agaagaagta aggctcgagc cgtaaatcca cacaccgtcc cccgaaaggg
      121 ggacgtttcc gtagaaaatc tggtacactt tcggtagtag ttctgttcat tatgtctaac
      181 cgacatcttt caagatcaat cgttcttcag tcgctcttcg agtgggattt gaataatata
      241 gagaaggagc acgttaagga ggccctcgaa cgaaatgtcg aggagttcgc gccgcataag
      301 gcggacatgc cgttcatgga gaggcttctc gacggcgtac ttcagaagca gaaggaactc
      361 gacctcgtca tcgagaaggc cgcccccgag tggcctatcg agcgtatcgc cccggtcgac
      421 cggaacatcc tccgtctcgg gctcttcgag ctcctcttcg cggataggaa ggaggtcccc
      481 gcgaaggtcg ccatcaacga ggcgatcgag ctcgcgaagc aattcggtgg cgagaactcg
      541 agtcggttcg tgaacggcgt cctcggtgcg gtctacaagg agatcggtga accgggcaag
      601 gaggaggttt ctcgaaataa gcgaagaaag gatattccgt tcgaggagat ggagatcgag
      661 cgcctcgcgg gcgccgtcgt ctacgcggtc cacgagggga cgatgtacct cgctctcgtc
      721 cacgatatct tcggccattg gacgctctcg aaggggaagc tcgaggcgaa cgaagaggtg
      781 aaagaggggg cgaagcgctc cctcaaggac gagataggtc tcgatgtcga gatcgaagcg
      841 gagctcggca agaacgagta cgtggcgtcg cacccggaga agggtcgcat tcgaaagcag
      901 gtgacgtact tcctcgcgaa gtccgagtac gtgaacctta cgctcgagca gaaaggaggc
      961 ctcgacgacg cgcagtggtt caaggtcgcc gacatcctcg aactcaattt ctatgaggac
     1021 attcttccca tcgtcacgaa ggcggtcacg atgctcgtct cgaagcggac gtaggcaagc
     1081 gtcgcttgat tttccaattt cggagttcgt gctagtgtaa cgccatgacc gagccggaca
     1141 agataccaga gagcctcgag cgcctcgaaa cgataatcgg ggtagttttt cgtgaccgga
     1201 cgcacctgct taccgcgata acgcaccgct cgtatctcaa tgagcaccgg gaggcgacgc
     1261 aggaacataa cgagcgctac gagttcctcg gcgacgcggt cctcgagctc atcgtgaccg
     1321 acttcctctt cagaaaatac ccggcgcggc cggaagggga actcaccgcc atccgcgcgg
     1381 ccctcgtcaa tacgacctcg ctcgccgagg cggcgacgaa cctcgcgatc aacgacttcc
     1441 ttctcctctc aaagggcgag gagaaggata ccggccgcgc gcgtcagtac attctcgcta
     1501 acacgttcga ggccataatc ggcgcgatct atctcgatat gggatacgac gccgcgctcg
     1561 acttcgtctc gcggcagctt ttcgggaaga ccgacgtcgt cgtcgagaaa caactctggc
     1621 gtgacgcgaa gagccgtttc caagagctcg cgcaggagca cgagagcatc acaccgagct
     1681 atgagacgct ctcgcagtcc ggtccggacc acgaccgcgt cttcacggtg ggcgttttcc
     1741 tcggtggcgc gaaggtcgcc gaggggaagg gccgctcgaa gcaggaggca gaacagctcg
     1801 ccgcggaggc gggcatccgt gcgaagcggt gggactaggg ccattaacac ggacgcgaag
     1861 aggaggcgaa ggctcatggt aaaataacgc catgggtttt ttaatgcgtg cgaaaacggg
     1921 cggctggacg atggccgaga tgatcatcgt tatcgccgtc atcgcccttc tcgcatcggt
     1981 cgtgattccg aatcttgtgg ggagtaaagg ccacgcacgc gacacgtcgc gcatctcgga
     2041 cctcaagcag atacagctcg cgtttcggac atggcgcgac gactactcga cgtcgacgta
     2101 ccccgtctac gcgtctggcg acgttatcgg cgacggtgtc ggggtcgaaa cggtcttcgc
     2161 gccgtacgtc ggcggtacca tcaaggaccc catcgatagc ggttcgaatc gctactacta
     2221 cgacagtgac tacacctgta acggcgcaag ccacgtcatc gtcatggcgc ttacgatgga
     2281 ggaggcgacc tccgggaact acgagagcgt ctgcggtgcg ggttcggagg gcgacctcgg
     2341 gaacggcgtg acgccgaccg ccgcgtcgta cgtcgttatc ctcaagtgac catgtatctc
     2401 aaggaactca ccatcaacgg attcaaatcg ttcgcgaaga agagcgacct cgagttcacg
     2461 acgcccatca ccgcgatcgt cgggcccaat ggttcgggga agtcgaacgt cgcggaggcg
     2521 tttcgattcg tcctcggtga gcagtcggtg aagtcgatgc gcgggaagaa gggggaagac
     2581 ctcatctggg gcggctcgga gagcgtttct cgctcgaacc gcgcgtcggt cacgatcaag
     2641 ctcaataatc cgtcgcggaa cggttcgcgc gtctttcccc tcgatttcga tgagatcacg
     2701 atagagcgcg tcgtgaaccg cgacggtacg aacgagtact cgataaacgg ttcgaagacg
     2761 cgtctcaagg acgtccagga gcttctcgcc gcggcgaaca tcggaccgac cggccaccac
     2821 atcatctcgc agggcgaggc ggaccgtatc ctctcggcga acccgaagga acgccgcgag
     2881 atgatcgagg acgcgttggg cctcaaggta tttcaattca agaaggcgga agcggagcgg
     2941 aagctcaaga agactcgcga gaacatcgcg caggtcgagt ccctacgccg ggaggtcgcg
     3001 ccgcacctca ggttcctcga gaagcagata aagaagatag agaagtcgct cgcgctccaa
     3061 gaggaactga agaccgtcta cgcggagtac ttgaagcgcg aggacacgta catcgcgttc
     3121 cataccgatc gcctcaccga agagcgccgt gcgccagcgg cacgccgtga agagctcaaa
     3181 gtgctcgtcg ccgccgcgaa ggagaaactc caagcgagag agaaggacga tacggcggac
     3241 gcgctcgtcg ccctcgagga gaagatgtcc gcggcccgcg cgaagcgcca ggagctcgtt
     3301 cgcacgatgg gtcagctcga aggccagata ctcttcctcg agcggcaggc ggcccagcgc
     3361 gagaaacggc gtgagtccgc ggccgaggag ccggtcccgc gaagcgaggt gaagcgcgtc
     3421 ttcgacgacg cggagcgcgc ggcgtcatcg gcgggtgaag acgcgaaggc gctccatcgc
     3481 gcgctccatg acaccgtcgc gtcacttaaa atgctcctca cgcacgcgtt ccacgatgag
     3541 aaggacgccg aagagagggc gtccgcagag gaactctccg cgctcaagga gacgaagtcc
     3601 gaatcggagc gtgacgtcgc cgcggtcgag aaggaagaag cggagctctc gcgcgaatac
     3661 gccgaattga aacggaaggt cgagtcggag aacgtcgaga gccgcgaggc ggagcgcgag
     3721 atattcgcgc tcatgaacga ggagcgagag gtcgagcaga agctcgacgc gatcgaccgc
     3781 gagctctccg tcctcgtgcg cgaccgcgac gagttcaaga acgagctcca ggaggccgtc
     3841 tcgctcatcg gacgctcggc gagcatgtat ttcgaatacg aggtgaaggg tgaggacggc
     3901 gcggcgctcc cggcggccgc gatgaccgcg gaggagcgga gcgttcaagt ggggaggcgc
     3961 cgcgagctcg agcgcatgaa gatacgcctc gaggaactcg gtaccggcgc gaacgaagac
     4021 gtcgttaagg agtataagga ggtgcaggag cgcgacgagt tcctcgttca cgagataacc
     4081 gacctcaccg aatcggtcgc gaagctcgag gcgctcatcg aggagctcac gaacgatctc
     4141 aatgaacaat tcctctccgg tatcgagaag ataggcgagg agtttcgaaa gttcttcacg
     4201 ctcatgttcg ggggcgggag cgcagaactt cttcgcgtga aaccgaagcc gaagaaacgc
     4261 gaggaagggg atgaggaagc tgacgctgcg ctcgccgacg aaggggagga ggtgtccgaa
     4321 gaatcggagg aagggatcga ggtcgacgtg aagctcccga acaagcgcgt tcgcggtctc
     4381 gatatgctct cgggcggcga gcgcgcgctc acgtcgatcg cgctcatctt cgccatgagc
     4441 caggtcaatc cgcccctctt cgtcatcctc gacgagaccg atgcggcctt ggacgaggcg
     4501 aacagccgcc gctacggcga tatgatatcc gccctcgccg agaagtccca gctcatcctc
     4561 atcacgcaca accgcgagac gatgggacgc gccggcgtcc tctatggcgt tacgatggcg
     4621 ggggacggcg tctcgaagct cctctccgtg aagttcgagg aggccgtcgc cgtggcaaag
     4681 tagtttgaca tcgccttact ttgaggttat aataatagca gttactcgta caggagactc
     4741 gattgaatac aagcggcagg cattgcgacg tcgtgatgta cgattccttc caacggttcg
     4801 aaaaaggtcg tgtggagtac gaaattgagg tccatcaata tccgacattg gaaggcgagc
     4861 cgaaagaagc acgccttccc ggagataaag aaatcgtacg gctcggaaaa aaatttctcg
     4921 ggcaacactt ccttcagatc aagcggagaa gggaaggaac gttcggccct gcgctcgaca
     4981 tcatccttct cccgggaagt ctcgcggagg accgcgctgc attcttggca gcattcgcta
     5041 cgcgaattcg ttaccgctga acccgcactt gaggaaactc ctgttgcggg ttttctcatg
     5101 tggaaaatct ccctttgaca cagggcgatt gttatggaga atatacgcac cgcttcaggg
     5161 gaagcggcat acacaaggag gtaagcatgc cgaagtgcac ggaagacgag cagtttccct
     5221 gccagacctg cgccgcctgt gagctgcggg cgaagctccg cccgtttctc gactggagcg
     5281 gcagacagcc gatccacacc gacaaggcgc ggctcggcaa cttcggtccg cactgaacca
     5341 ccttcaccaa aaggcccgcc tgcgggcctt ttttcttcgt acccacgatg agggggggtg
     5401 actccattga cttgacaata ggtcgagagt atgagaaatt cctcccactg cgttgtttcg
     5461 acagaggagg aaaatggcca agaacaagtg cagtttgtgt tccggggcgt tcaaagtgtt
     5521 cgccccagag aattgccccg cgtgcggtgg cggcggtgcg agcgcgagag tcgcgttcat
     5581 ccgcccgaag gcgacgccgg ggaagggtgg ggcggcgaag cgcctcccgc ccgaggttct
     5641 cgcgaacgtg aggagctcag aggtcgagca cgcgttcgcc tgaagtcgaa ggaggaggag
     5701 aaaaacgccc gtgcgcacgc gcacgggctc ttttttaaat gaaaaaagcg actctctacg
     5761 ctaattcgaa atcgaggacg cgttcctcca tgtccgcgcg gaccaacttc accttcactt
     5821 cgtcgccgag cgagtacgtc ttcccggtac ggttgccctt cacgcggtat ttcgtcgcct
     5881 catgcgtgaa gtagtcgttc ttcatcgacg agaggcggat cataccctcg gagcgcgaga
     5941 ccttgtcctc gacgtagatg ccccagtcgg cgacgccggt cacgaccgcg tcgaagacct
     6001 ggccgacctt gtcctgcata tactcgacct gcttgaactt gatggagtcg cgctcggcgg
     6061 tcaccgcggt gagttcacgc tccgacgact ggatggagag cgattcgtac ttcgcgatct
     6121 cgcggccgcc gatcggcgag ccgtcgaggt gcttccgaag gagccgatgc gccatgaggt
     6181 ccgggtaccg gcggatgggg gacgtgaagt gcgtgtagaa cttgaacgcg aggccgaagt
     6241 gcccgatgtt cttcgtcgtg tacacggcct tcgccatcga gcggatggtc gccgttttga
     6301 tgaggttctc ctcgggtgtc ccctcgatcg acttgaggag cttgttgatg tccttcgcgt
     6361 cgaccgagcc tttgtgcgtc ttgaactcgt accctaacgc atggaggaag agggcgaggt
     6421 tctctatctt ctcggggttc ggcacgtcgt ggacgcggta cacgaacgcc gcgtcgcgcc
     6481 ccttcttctt cgtatggtta tagacgaact ccgcgacgcg gcggttcgca agaagcatgt
     6541 aatcctcgat catgagcatc gtctcggtgc gcaccttcgc gtacacgtgg atgggttttc
     6601 cgttctcgtc gagctcaaag cggacctctg gttgatcgaa cgcgatcgcg ccctcctcat
     6661 agcgctcctt tcgaagtttt cgtcctatcg ccatgagctc gtggagctcc tctttgagcg
     6721 gaccctcgtt cgtgtcgagg acctcctgcg cttcctcgta cgagaagcgc ttgttcgagt
     6781 ggatgagcgt ttggccgaac cattcgccga gtatcgcgcc gtccttgttc atctcgaaga
     6841 ccgcggagaa ggcgagtcgg tcctcgttcg ggcggagcga gcagaggtcg ttcgagagaa
     6901 cttcgggaag catcgggatg acgcggtcga cgaggtagat cgacgtgccg cgcgactgcg
     6961 cctctttgtc tatcgtgtct tcctcgcgaa cgtagtgcga gacgtcggcg atgtggatgc
     7021 ctatctcgaa gttgccgttc tcgagtttat ggaaggagat cgcgtcatcg aagtccttcg
     7081 cgtcgaccgg gtcgatcgtc atcgtggtgc ggtcgcgcat gtcgcgccgc ctcgggtttg
     7141 ggcccttcgt cgcctcctcg gcctccttga atatctcatc gcggcgcgcg tagagctgtt
     7201 ttccggcctc ggcgacgtgg ggagggaagt tctcggagaa gccgccggag cggatgatcg
     7261 cctgcatctc cgtctcgtga tcgccggcgc ggccgatgac ctcggtgatc tccgcgatcg
     7321 gttccgagag cgggttcttc cattccttga tctccgcgac gaccttcatg ccctcgtcct
     7381 gtttcgtcgc gtcggggagg gcaagtcgga cgtggatgcg gcgattatcc ggctggagct
     7441 tatagccgcc gtccttcttc acgaccgtgc cgatgaactc gcggcgcttc acgtcgatga
     7501 cgcggaggac cttgccctcc tcacgcttcc cggggacgag cttctgtatc tcgatctcga
     7561 cgatgtcccc gtcgagcgcg aacccgaggt tctcgggcgc gatgaggatg tccgcatcgt
     7621 ggccgggcac ggtgacgaag cccgttccct tgccgcgtat catgatggcg ccctcgtacg
     7681 ttgccttttt agtgtccatg tggtcgtgag tgtaccgtag gcctctcgtg ttgtgaagca
     7741 tacatgacgt agctacgccg cgccatgaac ggtcggggtt gaccgatacg gctcattctg
     7801 gtagtctttc gggcacttat gttaaagatg cgattacagc ggatcggccg gaagaaccag
     7861 cccgaatacc gtgtcattgt caccgactct cgcaatgcgg cgaagcgcgg catgagcgtc
     7921 gacacgctcg gctcctacca gccaaagctc ggcgtcttca agatcgacgg tgaccgtgcg
     7981 aaggactgga tatcgaaggg cgtccaggtc tcggataccg tccacaactt cctcgtctcc
     8041 gagaagatca tcgagggcaa gaagaagaac gtcctcccga agaagtcccc gatcatcgac
     8101 gaggagaagc tgaaggcaga ggccgaggcg aaggcggcgg cggaagcaaa ggcggcggag
     8161 gaggcggcag cggccgcagc tccggtggaa gaggcgccgg cagagactgc gagcgaagta
     8221 cccacggaaa ctcctgtctc cgaagcatcg gcggaagcgg caccggcaga accggcagct
     8281 cccgcggagg agaaggctcc cgaagagaag gcggagtaac atcattcgaa cacgtgaaaa
     8341 agaccccgca cgcggggtct ttttcgaatt ctcacgattc cgagtttgac gcatcgttct
     8401 cagctgatac catagtcagg ttgcgtttca gagaacgtga cgcgcttacc agctagtttc
     8461 tagatcctat ggactcggaa gtaaacgcat caccgatggc ggcagaacct gaagagcagg
     8521 cgacgccgga gtcgaccgaa gagacgacca ccgaggctcc cgcgcctatg ccggaagagc
     8581 cggcggcgga gtagcacgtt cgaaaagacc ccgctcgggg tcttttcatt ttgtcttcac
     8641 gtccgtcgca tcaagtgccc gcagtactcg gttgacgtat atcgtcgacc tgctaccatt
     8701 tagcgagtta cgtttagtaa agaacgtcac gaccgcttaa cagattagtt ctcaacacat
     8761 ggaatcagat cagcagtttc tagaaacgtt agtgaaggcg ctcgtcgatc acccggaaag
     8821 cgtgaagatc aaccgcacgg tagacgagat gggcgttctc ctcaccctcg acgtgcacgc
     8881 ggaggacatg ggtaagataa tcggtcgctc gggcaacacg gcgaaggcta tccggacgct
     8941 ccttcgcgtc gtcggtatga agaacgaggc gcgcgtgaac ctcaagataa acgagccgga
     9001 gggcggtacg cgctccgcgc agacagacat ggcgtcggac gcgaacaccg acgagacgcc
     9061 ggcggggccg aagtcattag acgaggcgat cgacggccta aggatctaac actggagaat
     9121 ttctttgtcg tgggtacgat tttctcagtc acaataggat ttctattgct tcttccaaaa
     9181 tcttcccgct cctcgaactt ctctcagtct tagaccttag gagacacaac gcccgcactt
     9241 cgtgcgggcg ttgtgttgaa ctagctgaag aatcatgcaa cactacctcg catgacgaag
     9301 ggcatcgact ataccggtat ttccgtcgtc gcgctttgcc acgacgggaa gggaaagtac
     9361 ctcctcgaac tccgaagcga gaagtgccgc gacgagcgtc ttcgctggag caacgtcggc
     9421 ggcggcggac tcgaggccca tgagaccctc gaggaggcgg tccggcgtga gataaaggag
     9481 gagtgcggcg cggacgtgct cgtcatcgag cctctcggtt ttcgcgaggt cttccgcgag
     9541 agcgacgacg gtacgaagag ccactggata gcgttcgact tcaaagttga gatagaccca
     9601 gcacaagtgt ccgttcggga gcccgagatg tgtctcgagc atcgatgggt tgcgctttcc
     9661 gaattccccg agccgctcca ttcacagttc ccgcactttc tcgagaagta tagggataga
     9721 ctatagacat gcgatttcac gtcatcacca tctttccgga ggtctgcaag gcgtacaccg
     9781 acgcgtcggt tcttggccgt gcgcagaaga ccgagaaagg gaagggcgcg aagcaccgag
     9841 gaaagaagct tgaggtgcgg tacgtgaacc ctcgggactt cgcgaagaac aaacataaga
     9901 aagtcgacga tcgtccgtac ggcggtggcc ccggtatggt catgtacgcg gagcccatca
     9961 tccgcgcgtg ggagaaggcg gtcggaaagc ggaaggggaa ggtgaagacc ctcatcatgt
    10021 cgccgcgcgg gaagacgttc accgcgaaga tggcgaagga cctcgcggag aagttcgatg
    10081 acgtcgttct catctccggg cgttacgagg ggatcgatgc gcgcgtgaag aaggtgctca
    10141 aggcagaaga ggtgtcgatc ggcgactacg tgctcacggg cggcgagctt ccggcgctca
    10201 ccgtcatcga cgcggtcgcg cgccatatcc cgggcgtcct cggcacgttc gagtccctcg
    10261 aggacgagcg gatttcctcg ggcgtcatgt acacgcgccc cgaggttctc atccacaaag
    10321 gcaagaaata caaggttccg ccggtcctcc ttcgggggaa ccatgccgaa atagagtcct
    10381 ggcggaagac ggcgaagtaa tgaaaaacgg ttttgagccg tcgattcgtc aagtaagggg
    10441 aagcagcggt tgacgtcaag cgccgtatct gttaatatct cgcccatacg aaatcggctg
    10501 agtgcgttct ggttgcgcga ttttcataca aaaggttggg ggatgggggt ggtgtcgggg
    10561 gacaccgaaa caacgaggcg aatgcctcac acataatcac gtcacgtgat gtacacagta
    10621 caggctgagt aataagtgta tggacgaaac gcgcctcggg tgcgggtttt tacgttcatc
    10681 gcttcgctca ccgttattaa cggcgtaacg agtattcatt actatgtcca atgcaccgaa
    10741 ggcgaaggag aacgagggcg cggtccttcc gaagaagcac tttcagcgc
//
LOCUS       DASTSP010000064         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1908672, whole genome shotgun sequence.
ACCESSION   DASTSP010000064 DASTSP010000000
VERSION     DASTSP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908672"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFS75_02825"
     CDS             <1..246
                     /locus_tag="VFS75_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677625.1"
                     /translation="SVSTAPPSTPLIVLRTTDLSEDKRKLVAALGIADEEISITPEML
                     SCAEAKVSVPRLADIARGAAPSASEATSLAACYRDNR"
     gene            304..2211
                     /locus_tag="VFS75_02830"
     CDS             304..2211
                     /locus_tag="VFS75_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEU4677626.1"
                     /translation="MKKRSLRAARTAVAAIAAVSVALLCIIVAERVLSERTEPPAPPA
                     DAPYLNPDLPINVRVNDLLSYMTLEEKAGQMALVDKNSVHDDRDLATYGIGALLSGAG
                     AKPSGDDTAMSWRDMVARFTDASRESRLKIPVLYGVDAAHGHANVPGATVFPHEIGLG
                     AAGDPKLVRAVARATAAEMSATGIYWNFAPDLDQPRDIRWGRTYEAFSDDPELVSLLG
                     AAYVRGLEEPVLGGASSTVNVLATAKHFVGVGSMAWGSSSNRDFKIDQGTTPPDEDAL
                     ETEYLPAFREAVAAGVSSVMVGLNSWGDTKLAADRHLITDVLKGDLGFDGFVVSDWYG
                     VYEIPGNDYSSAVTAINAGVDMVMLPFDYEPFVRNVVRAVEDGDIPEARIDDAVRRVL
                     RAKFALGLFDGRTPGDGSVVGSKSNRALAREAVARSLVLLKNDRDTLPLRNVETIRVA
                     GSAADNIGRQAGAWTVEWQGIDGNWLPGATSILEGIRERAGANVTVAYDKNGDFPTGD
                     RADVGIAVVGEAPYAEGWGDNARPSLTNDDRAAIAKLRASCDEVVVIIVSGRPLLISD
                     ELSSWDALVAAWLPGSEGEGVADVLFGDEPFSGVLPVPWPDHEAQFSIAVDGSTADHT
                     AVLFPRYFGLH"
     gene            2320..2472
                     /locus_tag="VFS75_02835"
     CDS             2320..2472
                     /locus_tag="VFS75_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEU4677627.1"
                     /translation="MLYTIAVLLLILWLLGLVSSYTLGGFIHILLVVAIVMILIRLIR
                     GENPIR"
     gene            2511..2750
                     /locus_tag="VFS75_02840"
     CDS             2511..2750
                     /locus_tag="VFS75_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEU4677628.1"
                     /translation="MAKYGKAASKKVKKVMREYKRGTLKSGRSGKKVKSRKQAVAIGL
                     SEARREGDKVPKRKTSRKKSKTSSSNRTTKRRTKK"
BASE COUNT          547 a          907 c          870 g          511 t
ORIGIN      
        1 agcgtctcaa cggcgccgcc gagcactccg ctcatcgtcc ttcgtacgac tgatctctcg
       61 gaggataaac gaaagctggt cgccgccttg ggcatcgcgg acgaggagat ctccatcaca
      121 cccgaaatgc tctcctgtgc cgaagcgaag gtcagtgtcc cgcggctcgc ggacatcgcg
      181 cgcggagcgg cgccgagcgc gagcgaggcg acatcgctcg ccgcatgcta ccgagacaat
      241 cggtagcatg tcgtttctct gagcacgtga cgaaggttcg ttggggggat gtatactagt
      301 ttcatgaaga aaaggtccct acgagccgca cggaccgccg tcgccgccat cgcggcggtt
      361 tcggtcgcac ttctttgcat aatcgttgcg gagcgcgttc tctccgagcg caccgagccg
      421 cccgctccgc ccgcggacgc tccgtaccta aacccggatc ttccgatcaa tgtgcgcgtt
      481 aatgacctcc tctcgtacat gacgctcgag gagaaggccg gccagatggc gctcgtcgac
      541 aagaacagcg tccatgacga ccgcgatcta gcgacgtacg gcatcggcgc gctcctctcc
      601 ggcgcgggcg cgaagccgag cggcgacgat accgctatga gctggcgcga catggtcgcg
      661 cgattcaccg acgcgtcacg agagagccga ctcaagatac ccgtgctcta cggtgtcgac
      721 gccgcgcacg gccacgcgaa cgttcccggc gctaccgtgt ttcctcacga aatcggcctc
      781 ggcgcggccg gcgacccgaa gctcgtccgt gcggtcgcgc gtgcgaccgc ggcggagatg
      841 agcgcgaccg gcatctactg gaacttcgcg cccgacctcg accaaccgcg cgacatacgg
      901 tgggggcgaa cgtacgaggc tttctccgac gaccccgagc tcgtctccct tctcggcgcc
      961 gcgtacgtcc gcggcctcga agaaccggta ctgggcggcg cgtcctcgac ggtgaacgtg
     1021 ctcgcgaccg caaagcattt cgtcggtgtc ggttctatgg cgtggggcag ctcgagcaat
     1081 cgcgatttca aaattgacca ggggacgaca cctcccgatg aggacgccct cgagacggaa
     1141 tacctacccg cgttccgcga agcggtcgct gcgggcgtct cgagcgtcat ggtcggcctc
     1201 aacagttggg gcgacacgaa gctcgccgcg gataggcatc tcatcaccga cgttctgaag
     1261 ggagacctcg gtttcgatgg cttcgtcgtc tccgattggt acggcgtcta cgagataccc
     1321 gggaacgatt actcgtccgc ggtgaccgcg ataaacgccg gcgtcgacat ggtcatgcta
     1381 ccgttcgatt acgagccgtt cgtgaggaac gtcgtccgag ccgtcgagga cggcgatatc
     1441 cccgaggcgc gtatcgatga cgcggtgcgc cgcgtcctcc gcgcgaagtt cgcgctcggc
     1501 ctcttcgacg gccggacacc gggcgatggc tccgtcgtag gatcgaagtc gaaccgagcg
     1561 ctcgcgcgcg aagcggtcgc tcgctcgctc gttctcctca agaacgaccg cgacacgctc
     1621 cctcttcgaa acgtcgagac cattcgcgtc gcgggaagtg ccgcagataa catcggtcgt
     1681 caggcgggtg cgtggaccgt cgaatggcag ggcatcgacg gcaactggct tccgggcgcg
     1741 acgtccatcc tcgaaggcat acgagagcgc gcgggtgcga acgttaccgt cgcgtacgat
     1801 aagaacggag acttcccgac cggggaccgc gccgacgtcg gcatcgcggt cgtcggcgaa
     1861 gcgccgtacg ccgaaggctg gggcgacaat gcgcggccgt cccttacgaa cgatgaccgc
     1921 gccgcgatag cgaagctccg cgcgtcgtgc gacgaagtcg tcgtcatcat cgtctcgggc
     1981 cgaccgctcc tcatctccga cgagctctcc tcatgggatg cgctcgtcgc cgcatggcta
     2041 ccgggtagcg agggcgaggg cgtcgcggat gttctcttcg gtgacgagcc atttagcggc
     2101 gttcttcccg tcccctggcc cgaccatgag gcgcagtttt cgatagcggt ggacggttcg
     2161 accgcggacc acaccgcggt cctcttcccc cgatacttcg gactacattg acgcctcacg
     2221 aattgttcat ctttttttca cgatagcgcg cgtacaattc tgaaacggca tcggaggagc
     2281 tggtcacgcc tcccttttta tcagtagtac aacccacaca tgctctacac tatcgcggta
     2341 ctgcttctca ttctctggct cctcggcctc gtgagctcct acacgctcgg agggttcatt
     2401 cacatacttc tcgtggtcgc catcgtcatg atcctcatcc gactcatccg cggggagaat
     2461 ccgattcgat gacgattcga tcattaccag ttccttcgta gttcattcat atggcaaagt
     2521 acggaaaagc ggcctctaaa aaggtcaaga aggtcatgcg ggagtataaa cgcggaacgc
     2581 tcaaatccgg gcgaagcggc aagaaggtga aaagccgcaa acaagcggtc gcgatcggcc
     2641 tctcggaggc gcgacgcgag ggcgataagg tcccgaagag aaagacgtcg cgaaagaagt
     2701 cgaaaacgtc gtcctcgaac cggacgacga aacgacggac gaagaaatag acgcgatgaa
     2761 agaacgccct cgggcgttct ttcatcgcca gggtaggcgc gtacgccaga gatcgcacgg
     2821 catgagcccg ttcca
//
LOCUS       DASTSP010000065         7097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_568437, whole genome shotgun sequence.
ACCESSION   DASTSP010000065 DASTSP010000000
VERSION     DASTSP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568437"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFS75_02845"
     CDS             complement(<1..411)
                     /locus_tag="VFS75_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677629.1"
                     /translation="MRFLYRLLDHIYAQSPETKSRYAFAGAAVITGVIAIIWVSTIPS
                     RFATTAEIAPETNTTAKDFVDAWQKTTDQMGTAISSLGTAAGQEDPAAARNDEPDVTA
                     ASEPIPGALGSLTSDESASTSVSEGERSDDPHSVI"
     gene            complement(436..1056)
                     /locus_tag="VFS75_02850"
     CDS             complement(436..1056)
                     /locus_tag="VFS75_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4677630.1"
                     /translation="MIITYHGMQCFKVSFGDTTIAFDPISKKSKLSGPKFGADVVFIS
                     LRHPDMNGADEMAFGSKQPFVVDGPGEYEVGQVTARGYGVKTMYEGVERFNTIYKVTF
                     EDMNLIFLGALGSEEIDPKILGELDDIDILFVPIGGGDVLDVPAASKLATKLEARCII
                     PMHYADAALKAFLKEEGVDAGKPVDKLTVKKKDLADKEGDIVVLAA"
     gene            1262..2449
                     /locus_tag="VFS75_02855"
     CDS             1262..2449
                     /locus_tag="VFS75_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU4677631.1"
                     /translation="MSDEKASIFDRISSLVHGGSDAPADEAENDAPTMTAEEEARTKS
                     PMNSLLEQSPVPPKEGDLVEGKVTAIGRARVYIEIAPFGTGIIYGREFMNARDILRKV
                     SIGDSIAAKVVDAGNEDGYIELSLKEARQALIWSDAEEAVKKQTIMYLPVKEANKGGL
                     IIEWQGIQGFLPASQLSSEHYPRVEDGDKDKILSALNDLVGKSLSVIIITADKKEGKL
                     IFSEKGPQEKEEKEEKVSKYTVGDVVDGTVTGAVDFGVFVKLEEGLEGLVHISELDWG
                     LVEDTRALYKVGDKVKVKVIEVKDDKISLSIKQLKENPWHGAAKKYKKGQEVQGVIIK
                     YNKHGALASIEEGVAGLVHISEFASEEELRRTLSLGSTYAFIITLFEPKDQRMTLSYK
                     KGQ"
     gene            complement(2720..3310)
                     /gene="pth"
                     /locus_tag="VFS75_02860"
     CDS             complement(2720..3310)
                     /gene="pth"
                     /locus_tag="VFS75_02860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU4677632.1"
                     /translation="MFTIVGLGNPEKEHASTRHNAGWLTLSRFIEENGLPEASRSSRY
                     EGLLSEGQVGGRAVAVLMPTTYMNNSGTSVAKYVKEKGALDELVVVHDDVDLAWGDVK
                     ISAGRGAGGHNGVQSVIDHLGTKEFVRVRVGIAKRGFFGGVKRPTGEDLAAFVLSPFS
                     AGELKALPDIAKRVADALTTILEHGAVRAMQEVNGL"
     gene            3405..4052
                     /gene="lepB"
                     /locus_tag="VFS75_02865"
     CDS             3405..4052
                     /gene="lepB"
                     /locus_tag="VFS75_02865"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU4677633.1"
                     /translation="MEHTPETENTRTTSPAPVAESTAAHPEPEKKESLIGEIVRFSLF
                     ALIIVLPIRLFIAQPFIVSGASMEHTFSTGQYLIVDQLTYRFDAPKRGDIIIFRYPKD
                     PSKFFIKRIIGIPGDTVTIDGDHVTIADAAHPEGETLKEPYILDMKPNPPLTEHLGDD
                     EYFVMGDNRNASSDSRSWGVLQRDKIIGRAFLRLFPLSEVGVFPGRFDIDAMNSL"
     gene            4071..5249
                     /locus_tag="VFS75_02870"
     CDS             4071..5249
                     /locus_tag="VFS75_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4677634.1"
                     /translation="MPSSTSVSIQDFVKSASHTAYHFGFSPLDRLRAHPACKTCDTRI
                     EHHANAQSRKNDSLHGMLTQGMCAYFDYKLHAIEGPSLFYTIDQVPRTGEVAVTFHVL
                     NVRKSIAEALLIQTIRSFLADLGYARQLVRINSIGDSDSISRYIRDLTNFLRKRLDEM
                     PPQARELMKEHPIAALLHLIEKEHDIAKKSPNPLEYLTDSSRKHFREIVEYLDMSETP
                     FEIDPRLIGHHECYSDALFAFDVIGENDERDLGAPFTIRGGRYSNFVSKMTRTKIPAA
                     GAVVILKDKKAPAHIPAPRVRYTPSVYLVQLGFGPKVKSLLLIDELRRAGISVFQNIM
                     SDSLSEQLRLAEAKNARYAIILGHKEFMDGNVILRDLTQQSQEYLPMASLASYLKKVA
                     "
     gene            5310..5870
                     /locus_tag="VFS75_02875"
     CDS             5310..5870
                     /locus_tag="VFS75_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677635.1"
                     /translation="MWHRLLDRVDDALIDRFFQPITDIVRRLTGFSKRIPCAVTLLLS
                     AATFIPTSIEMVGSGMPAAVFMGLFNCAVAFTAAYASFVFVSLELYERGRPSRADALN
                     NERVRFKENRRGLLPIGAALVAVGIILNVVDPNYSQVLPIIVGQLLLMAFVYFEACTN
                     LPPRRSILSKLKEAAGKFFLSPAPTP"
     gene            5960..6205
                     /locus_tag="VFS75_02880"
     CDS             5960..6205
                     /locus_tag="VFS75_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677636.1"
                     /translation="MATNVEVKKNNQESTANLIRRFTKRVQGSGILMRLRKRRYHTRQ
                     KSDLVNRSKKLVKLENKAKYETLLKLGKIQENPRGRR"
     gene            6262..6639
                     /gene="ybeY"
                     /locus_tag="VFS75_02885"
     CDS             6262..6639
                     /gene="ybeY"
                     /locus_tag="VFS75_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4677637.1"
                     /translation="MATTISSTVRRFPTLPYEAMKDAVLGKEYTLALTFIGERRAKVL
                     NVSYREATYVPNVLSFPLSDTAGEIYICPRVAAREAKKFSLSKNGYIGFLFIHGLLHL
                     KGHLHGDTMDKAEKRYVARFNLK"
     gene            6672..>7097
                     /locus_tag="VFS75_02890"
     CDS             6672..>7097
                     /locus_tag="VFS75_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU4677638.1"
                     /translation="MAQHYVTGIDVGTYQVKVVIAAEPERGSVPQIIGTGYAESRGLR
                     NGYIINENDVVRSVRNAVAQAEKASGVKVKRAYISMGGIGLDEIHSRGEAITSRADSV
                     ITQVDIDKAIQASEESIQDKIPNRKIIHAIPLTYTVDGEK"
BASE COUNT         1472 a         2240 c         2010 g         1375 t
ORIGIN      
        1 aatgaccgag tgaggatcgt ccgaacgctc gccttccgat acgctcgttg acgcagattc
       61 gtcgctcgtg agcgaaccga gagcacccgg tatcggctcg ctcgcggccg tcacgtccgg
      121 ctcgtcgtta cgagccgccg cggggtcttc ctgtcctgcc gccgtgccga gactcgagat
      181 agcggtcccc atctggtcgg tcgtcttttg ccacgcgtcg acgaagtcct tcgccgtcgt
      241 gttcgtctcg ggagcgattt cagcggtcgt cgcgaaacga gatggaatgg tcgataccca
      301 aatgatggcg atgacgccgg ttatgaccgc cgcgcccgcg aacgcgtacc gcgacttcgt
      361 ctcgggcgac tgcgcgtaga tgtggtcgag gagccggtag aggaagcgca tacggcacca
      421 gtatacaccc ggccgctatg cggcgaggac gacgatgtcg ccttccttgt cggcaaggtc
      481 cttcttcttc acggtgagct tatcgacggg cttccccgcg tcgacgcctt cctccttgag
      541 gaacgccttc agtgccgcgt ccgcgtagtg catgggaatg atgcagcgag cttcgagttt
      601 cgtcgcgagc ttcgaggccg ccggcacgtc gaggacatcg ccgccgccga tcggcacgaa
      661 gaggatgtcg atgtcgtcga gctcgccgag tatcttcggg tcgatctcct cggaacccaa
      721 cgcgccgagg aagataaggt tcatgtcctc gaacgtgacc ttatagatgg tattgaaccg
      781 ctcgacgccc tcatacatcg tctttacgcc gtacccgcgc gcggtaacct gaccgacctc
      841 gtactccccc gggccatcga cgacgaaggg ttgcttcgaa ccgaacgcca tttcgtcggc
      901 gccgttcatg tcgggatggc gaagcgagat gaagacgacg tccgcgccga acttcggccc
      961 cgagagcttc gacttcttcg agatcggatc aaacgcgatg gtcgtgtcac cgaaggagac
     1021 cttgaagcac tgcatgccgt ggtaggtgat gatcatgatg cgcctatagt actaaacgtt
     1081 tctccgttcg ggaaggagcg gtgaggctgt ctgcgccgtg gacagaggcg gtttttcgta
     1141 gtatactcgc cccacttcgc aagaagatta tcagttctaa cggcatgcgc gtaatcccgc
     1201 ggtcatgaac atggccacga gtaacggttt ccgcggatac gtatcgccga agaaaacaac
     1261 aatgtcagac gagaaagctt cgatcttcga tcgaatctcc tctctcgttc atggagggag
     1321 cgacgctccg gccgacgagg ccgagaacga tgcgccgacc atgaccgcgg aagaggaagc
     1381 acggaccaag agtccgatga actcattact cgaacagtca ccggtccctc cgaaggaggg
     1441 cgacctcgtc gaaggtaagg tgacggcgat cggacgcgcg cgcgtctaca tcgagatagc
     1501 accgttcggc accggcatca tctacggccg ggagttcatg aacgcgcgcg acattcttcg
     1561 caaggtctcc atcggggact cgatcgcggc gaaggtcgtc gatgcgggga acgaggacgg
     1621 ctacatcgaa ctctcgctca aggaggcgcg acaggcgctt atctggagcg acgcggagga
     1681 ggcggtgaag aagcagacca tcatgtacct cccggtgaag gaggcgaaca agggcggact
     1741 catcatcgag tggcagggta tccagggatt cctcccagcg tcccaactct cgtcggagca
     1801 ctacccgcgc gtcgaggacg gcgataagga caagatactc tccgcgctca atgacctcgt
     1861 cgggaagtcg ctctcggtga tcatcatcac ggccgacaag aaggagggca agctcatctt
     1921 ctcggagaag ggaccgcagg agaaggagga gaaggaggag aaggtctcga agtacacggt
     1981 cggcgacgtc gtcgacggta cggtgacggg cgcggtcgac ttcggcgtgt tcgtgaagct
     2041 cgaggagggt ctcgagggtc tcgtgcatat ctccgagctc gactggggcc tcgtcgagga
     2101 tactcgtgcg ctctacaagg tgggcgacaa ggtcaaggtg aaggtcatcg aggtcaagga
     2161 cgacaagatc tccctctcga tcaagcagct caaggagaac ccgtggcacg gcgcagcgaa
     2221 gaagtataag aagggccagg aggtccaggg cgtcatcatc aagtacaaca agcatggcgc
     2281 gctcgcctcg atcgaggaag gcgtcgcggg tctcgttcac atctccgagt tcgcctcgga
     2341 ggaggaactc cgtcggacgc tctcacttgg ttcgacgtac gcgttcatca tcacgctctt
     2401 cgagccgaag gaccaacgca tgacgctctc ctacaagaag ggacagtaga ctgcgcacac
     2461 gaaaaaccgc ctcacggcgg ttttttcgtt atactacgat cgtggtatcc aaggggtacc
     2521 acatatcgtc cgctcgcggc agaagaacga tactacattg tacaaagcat ggtcgaaacg
     2581 cggatgtggt cgccagatga cttgcattga cccgggtgtc gatgcttaag ccatggcaga
     2641 aggtccgcgt caccagatgc tttgcaagcc gctcccgtcg tgttccgaac gtccaagacc
     2701 tcgctccgtc gaggtctttt tatagaccgt tcacctcctg catcgctcgt acggcgccgt
     2761 gttcgagtat cgtcgtaagc gcgtcggcga cgcgcttcgc tatatccgga agcgccttca
     2821 gttccccggc gctaaacggt gagaggacga aggcggcgag atcctccccc gtcggccgct
     2881 ttacgccgcc gaagaacccg cgcttcgcga tgccgacgcg cacacggacg aactccttcg
     2941 taccgaggtg atcgatgacc gattgcacgc cattgtgtcc gcccgcgcct cggcccgcgg
     3001 atatcttcac gtctccccac gcgaggtcga cgtcgtcgtg gacgacgacg agctcgtcga
     3061 gcgccccctt ctccttcacg tacttcgcga cggacgttcc cgagttgttc atgtacgtcg
     3121 tcggcatgag gaccgcgacg gcgcgcccgc cgacctgacc ctccgagagg aggccctcgt
     3181 agcgcgacga gcgcgacgct tcggggaggc cgttctcctc gatgaatcgg gagagcgtga
     3241 gccatccggc gttgtgccgc gtcgaggcgt gttccttctc gggattgccg agtccgacga
     3301 tggtgaacat gatggaccca ttgtacatga gggtacgcac tacgacatac gcgaaacttg
     3361 tgttcaactt tcttcgcgat tataatgtct cggaatcatc acctatggag cacacaccgg
     3421 agacagagaa cactcgaacc acctcccctg ctcctgtcgc ggaaagtact gccgctcacc
     3481 cggagccaga gaagaaggag agcctcatcg gcgagatcgt ccgcttctcg ctcttcgcgc
     3541 tcatcatcgt cctccccatt cgcctcttca tcgcacagcc gttcatcgtc tcgggcgcct
     3601 cgatggagca caccttctcg acgggccaat acctcatcgt cgaccagctc acgtaccgct
     3661 tcgacgcgcc gaagcgcggc gacatcatca tcttccgcta cccgaaggac ccttcgaagt
     3721 tcttcatcaa gcgcatcatc ggtatcccgg gcgatacggt cacgatcgac ggcgaccacg
     3781 tcaccatcgc ggacgcggca cacccggagg gcgagacgct caaggaaccg tatatcctcg
     3841 acatgaagcc gaatccaccc cttaccgaac acctcggcga tgacgagtac ttcgtcatgg
     3901 gcgacaaccg gaacgcgagc tcggactccc gctcatgggg agtactgcag cgggacaaga
     3961 tcatcggccg tgctttcctt agactcttcc cgctctcgga ggtgggagtg ttcccgggaa
     4021 ggttcgatat cgatgccatg aattcgttat aagtctcacg tagttcgcat atgccatcgt
     4081 cgacatccgt atcaattcag gatttcgtaa aatccgcgtc gcacacggcc taccacttcg
     4141 gtttctctcc gctcgaccga ctgcgtgcgc acccggcctg caagacctgc gatacgcgaa
     4201 tcgaacacca tgcgaacgcg cagagccgga agaacgactc cctccatggc atgctcacgc
     4261 aggggatgtg cgcgtacttc gactacaagc tccatgcgat cgaaggcccg tcccttttct
     4321 atacgatcga ccaggtcccg cggacgggcg aggtcgccgt gacgttccat gtcctcaatg
     4381 tgaggaagtc gatagccgaa gcgctcctca tccagaccat ccgctcgttc ctcgccgatc
     4441 tcgggtacgc gcgccagctc gtccgcatca attcgatcgg cgattccgac tccatctctc
     4501 ggtacatccg cgatctcacg aacttccttc ggaagcggct cgacgagatg ccgccccagg
     4561 cgcgcgagct catgaaggag catcccatcg ccgctctcct ccacctcatc gagaaggagc
     4621 acgacatcgc gaagaagagc ccgaatcccc tcgagtacct caccgactcg agccgaaagc
     4681 acttccgcga gatcgtcgag tacctcgaca tgagtgagac gcccttcgag atagacccgc
     4741 gtctcatcgg gcaccatgag tgttactccg atgcgctctt cgcgttcgac gtcatcggcg
     4801 agaacgacga gcgcgacctc ggtgcgccct tcaccatccg cggcggccgc tacagcaact
     4861 tcgtctcgaa gatgacgcgt accaagatac cggcggccgg cgcggtcgtc atattgaagg
     4921 acaagaaggc accggctcac attcccgcac cccgcgtccg ctacacgcca tcggtctacc
     4981 tcgtgcagct cggcttcggg ccgaaggtga agagcctcct cctcatcgac gagctccgcc
     5041 gtgccggcat cagcgttttc cagaacatca tgagcgattc gctctccgag caactccgcc
     5101 tcgccgaagc gaagaacgct cgctacgcca tcatcctcgg tcataaggag ttcatggacg
     5161 gaaacgtcat cctccgcgac ctcacccagc agagccagga atacctcccc atggcctccc
     5221 tcgcctcgta cttgaagaag gtcgcctgat tgcgtacctg ccgcgcctcc tgtaccatcc
     5281 tctcggacaa tactgggagg attttcgaca tgtggcacag actcctcgac cgcgtcgacg
     5341 acgcgctcat cgaccgcttc ttccaaccta tcaccgatat cgtgaggcgc ttgaccggct
     5401 tctcgaaacg aattccctgc gcggtcacgc ttctgctctc ggccgccacg ttcatcccga
     5461 cgagcatcga gatggtcggg agcggaatgc ccgccgccgt cttcatgggc ttgttcaatt
     5521 gcgccgtcgc gttcaccgcg gcctacgcaa gcttcgtgtt cgtctcgctc gaactctacg
     5581 agagaggcag accgtctcgc gcggatgcgc tcaacaacga acgggttcgc tttaaggaga
     5641 accgacgcgg actcctcccc atcggcgcgg ccctcgtcgc ggtcggcatc atcctcaatg
     5701 tggtcgatcc gaactactct caagtgttgc ccatcatcgt cggtcaactc ctcctcatgg
     5761 cgttcgtcta cttcgaggcg tgcacgaacc tgcctccccg taggtcgatc ctctcgaagc
     5821 tcaaggaggc cgccgggaag ttctttctct cccccgctcc cacaccctga gtgacgggat
     5881 gagaaggccg cgcatgcgcg gccttttttc tgcccgtaac cttgcgaaag cgcgcgccgt
     5941 gtgctattct ctcgccacta tggcaacgaa cgtcgaagtc aagaaaaata accaggagag
     6001 caccgcgaac ctcattcgcc gcttcacgaa gcgcgttcag ggttcgggca tcctcatgcg
     6061 tctcaggaag cgccgatacc acacgcgcca aaagtcggac ctcgtgaatc gttctaagaa
     6121 gctcgtgaag ctcgagaaca aggcgaagta cgagaccctc ctcaagctcg gcaagataca
     6181 ggaaaaccct cgcggtcgcc gctagcaaaa tcccctccct tgaggggatt tttcgttttg
     6241 gacctgtgct acactctcga aatggccaca actatcagct cgaccgttcg tcgcttccct
     6301 acgctcccct acgaagccat gaaggacgcc gttctcggca aggagtatac gctcgccctt
     6361 accttcatcg gcgagcggcg cgcgaaggtg ctcaatgtct cctaccgcga ggcaacgtac
     6421 gttccgaacg tgctctcatt tcccctctct gataccgcgg gggagatata catctgtcct
     6481 cgcgtggcgg cccgtgaggc gaaaaagttc tctctgtcga aaaacggcta catcggcttc
     6541 ctttttatcc acggcctact ccatttgaag ggacacctcc atggcgatac aatggataaa
     6601 gcagaaaaaa gatacgttgc gcggttcaat ttgaaataac cgtcgcgttt ttcggcttcc
     6661 gctttttatt tatggcccag cattacgtca ccgggatcga tgtcggcacc taccaggtga
     6721 aggtcgttat cgcggcggag ccggagcgtg ggagcgtccc tcagatcatc ggtacgggct
     6781 acgcggaaag ccgcgggctt cgaaacggct acatcatcaa cgagaacgac gtcgtgcgga
     6841 gcgttcgaaa cgcggtcgcg caggcggaga aagcgagcgg cgtgaaggtg aagcgcgcgt
     6901 acatctccat gggcggcatc ggtctcgacg agatacactc gcgcggcgag gccatcacct
     6961 cgcgcgccga ctcggtcatc acgcaggtcg acatcgacaa ggctatccaa gcgagcgagg
     7021 agtcaattca ggacaagata ccgaaccgga agatcatcca tgcgattccc ctcacgtaca
     7081 ccgtcgacgg cgagaag
//
LOCUS       DASTSP010000066        16897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_800707, whole genome shotgun sequence.
ACCESSION   DASTSP010000066 DASTSP010000000
VERSION     DASTSP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800707"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..681
                     /locus_tag="VFS75_02895"
     CDS             292..681
                     /locus_tag="VFS75_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677639.1"
                     /translation="MTKITGAEKWLEEARGTLRRDPTSTLPCLTNHDGWNDAVSAVYS
                     EPENYEETLLSLEIRLVLQQACRNVHARTTANGYRPTRSDLAAAFRLGDIIAKTDPNI
                     TFSMKRLLHELMTDLGFTAERSPALEW"
     gene            776..1255
                     /locus_tag="VFS75_02900"
     CDS             776..1255
                     /locus_tag="VFS75_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677640.1"
                     /translation="MVRRIAYLLIALMPLPLYGKWVHAGIPFTYEAGDSYRYTLRDGT
                     SCYVGRLYAHPAVVFYVIGPVERLRAKGTTARYARRTYDEAVIDAESGKLREYNHAEI
                     VPRPYGTGYPEVLPDGDIDFPCKAFVAAIPSRYVRLGLTRFLERLERAQNSEELFLQ"
     gene            complement(1302..2957)
                     /locus_tag="VFS75_02905"
     CDS             complement(1302..2957)
                     /locus_tag="VFS75_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4677641.1"
                     /translation="MQQKTKEEIAALIEGEVRDDAATLERLSKDASIFTVRPSLAVAP
                     RSATDVEKLVEYVAKRNQEGERLTVTARSAGTDMTGGPLNEGIILEFTEHMNALREIG
                     ADSATVEPGMYYRDFEKATLAKGLLLPSYTASKDLCAVGGMVANNSGGEKTLVYGKTE
                     KYVRRLKVVLSDGSVAEFEKLTMKELEAKKKLDTLEGQIYRKMHELCESEYDLIKASK
                     PNVTKNSSGYYLWNVYDRETGTFDLTQVIVGSQGTLGIVTEIEFALVRPHTHTRLAVV
                     FLKEMKTLPKVIHELLSFTPESLESYDDHTFRFAIKLFPMIVKRLGGSIFGLALRFWP
                     EFKAVLTGGLPKLILLAEFSGDTDEEATAKAEASVAAMREMGYEARVTTGPEEAEKYW
                     VMRRESFNLLRQKKTDMRTASFIEDFAVHPDDLPTFLPKFYALLDEYDLTYTVAGHMG
                     DANFHIIPLMDMRRPDADDIISALMDKVYRLVLSYRGTITAEHNDGLIRSSYVPLMYG
                     DDVVALFERTKDIFDPHRIFNPGKKVFADRQYALEHMVHNNLG"
     gene            3094..4206
                     /locus_tag="VFS75_02910"
     CDS             3094..4206
                     /locus_tag="VFS75_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU4677642.1"
                     /translation="MKLRDIPKMEVQRHMSPEIQYMQSHAHLYVDRIIKNTRSFPQIS
                     SIDDLPPGAIRTLRGGRMSALYSVAIENELIVIKMRRDGIEAECEALEAWRRVKVPVP
                     TVLKMGTLADTKTMTGVKYLLLDFVLDRSGDPAPTGADYLERHPSAAHRVGRLLGRTL
                     ARMHRAKTDRTFGEYADMGHSNRSPYRSWNKYLLGYLDAYRPYLVSLGLTRKLVKRLE
                     TTIRDAEFGTVGTYVHGDYSLRNVMIDSRKPLRAVVFDPNPVIGDPSWDIAILMNNRD
                     YALRRYAWEPQDQAYRKIAKREERFLEGFWASYRNASRERVPLSHIRIAQLLQTLLHL
                     ATEERKEYARPEEKAVEIRSRRETLFELIGTLTMNS"
     gene            4222..4755
                     /locus_tag="VFS75_02915"
     CDS             4222..4755
                     /locus_tag="VFS75_02915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4677643.1"
                     /translation="MRVYFVRHGERADNSDSIFASRDIPITELGKRQAGLLAERFASI
                     PIDAIYASPYVRTRETAEIINAVKKTGIRYDARIEELGREEELANVFRRANSFVSFLE
                     NECVEERILVSAHAGIIKMIVFAMVFGELHLGGAFKEFDRSFHMDFTGITYCTREKPN
                     PWHIVSWNDLGHLPPPV"
     gene            complement(4791..5408)
                     /locus_tag="VFS75_02920"
     CDS             complement(4791..5408)
                     /locus_tag="VFS75_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677644.1"
                     /translation="MHGESLYASAIAAGIPDGIVAVFAAHPYAIIFLSLLVGGGIILF
                     PAFYLSLSGALSASSIIWVMVGAAIVNDSFWYAVGRGMLPRFIERRFIESRSDKVRRL
                     SRVVRGRELVVLFYSKFVYGTRIAAQILCGARKVSLPPFQLVGAFAVLILGCVYYGVV
                     RIAARSAESLTDARVKLAVVLFATALAMGTLHYLAYRIGKKRFGE"
     gene            complement(5496..6812)
                     /locus_tag="VFS75_02925"
     CDS             complement(5496..6812)
                     /locus_tag="VFS75_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HEU4677645.1"
                     /translation="MKRSLSVSFAIALAAFLFAGVADAAARCVFTTDLAIGSAGAPVA
                     CLQRMLLDHGIVIPAGATGYFGSQTKAAVSLWQSAHGVTPNSGYFGPLSRIAYVFDAP
                     TAVKAPTPVRAASTVVVRKATKPSPVKVVKKHASPHSGTSAPNAPTEERDGDGKAETP
                     TPSPTPTPAPTPSPTPTPEPKGDDGSSTDPAPTTAPTEETTPSVDVPADEGVVLWKCD
                     FEDSYCGMYEQSKVEPDGHRSSFVGTARNGERAVMLTTMPGDDEVHGSGYWERDDLSL
                     GTSPDYCNEGQEEWWAMSVLFPDSYVVPKEGGAVMDFHHNASGGQANFHFLSQPTGLR
                     MHGFYGDVSNPEEYKVDLGPVVRDQWYDLVYHVKWSSGDDGYFVAWMNGKKVLDHEGP
                     TLYKGISCYLKLANYHDPFGESSSIIFDRVMRGTSAQAVSRTPLEE"
     gene            7038..8684
                     /locus_tag="VFS75_02930"
     CDS             7038..8684
                     /locus_tag="VFS75_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4677646.1"
                     /translation="MTIVVILVSFHTASRADAATDPNLVPNPSFETAGGNGLPANWGK
                     GGYGTNDRAFTYPVSGYGGGKAARVEVKNYTSGDAKWYFAPVSVTPLQALLITDEYRS
                     DATSYVTVQIVHTDGRITYADLGKLDPSSTWTKFTGYYTPPADTVKVSVFHVIKSTGF
                     LETDDFFVGTTGPNLVPNPTLSTKTDAGLPANWQKGGYGANDRSFTYPVTGYDDGSAV
                     RVDIGSYSSGDAKWYFASVPVKPLQPLKFEDHYRSDRESYVTIQVTHDDGSNTYVDLA
                     KLAPASSWTSYTGYYTPPADASSLTVFHLIKGVGFLETDGYFLAQVLSDPNRFDRGYV
                     SLMFDDGVETTYKNAFPILDAANFKVDTFIITDRLKDGTYPGYVKAADVIAMQNAGHE
                     IGAHTRSHPDLTTLSPADAKAEIEGSRNDLLAIGAKPVNFFAYPYGAYNTPVIDDVKA
                     AGFLGARSSDGGYNLKTGNQFALRRISMDRTTTLADVKQYVETAKEDKTWVIFLFHDV
                     DDSGSEYAVSPALFKEIVDYLQSAGVTPITMSKGISLMNQ"
     gene            8757..9218
                     /locus_tag="VFS75_02935"
     CDS             8757..9218
                     /locus_tag="VFS75_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU4677647.1"
                     /translation="MNANEFLKKQHRDVQALYEEFKAADGDAKDEIGKKLLTALVVHD
                     DIEDELYYPELEKVGLKDSVEEYRAEHVATETNIGRLKTMDHEDDEFEPTMKAMMEGV
                     NHHIEEEETQGMPKFEALVDPGVLADLGAKLDARSKELHESTLKRLWATLT"
     gene            complement(9337..9585)
                     /locus_tag="VFS75_02940"
     CDS             complement(9337..9585)
                     /locus_tag="VFS75_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU4677648.1"
                     /translation="MMNYGYYGHGYGWELSGMIGTVLFWALVVAGIVWLVRASRGHHY
                     DGHHCGRGSNAIDILKERYAKGEIGKEEYDSKLADLSK"
     gene            complement(9666..10097)
                     /locus_tag="VFS75_02945"
     CDS             complement(9666..10097)
                     /locus_tag="VFS75_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU4677649.1"
                     /translation="MTSLLPEIELLFMLNLALSLLAGFLIGLERESRGKDAGISTQSL
                     VIMGAMLFTFISAHIEPDFTARIAAQVVMGVGFLGGGLILKEGKAVHNLTTAASLWYS
                     AAIGMCFGFGFHVIGIFSTLIGVLVMRIPHIAMPRRDDTHA"
     gene            10287..11558
                     /locus_tag="VFS75_02950"
     CDS             10287..11558
                     /locus_tag="VFS75_02950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4677650.1"
                     /translation="MHNAHRQGRSSSSRFARQGGRGRNTFHASGRRSGGRQTNHRGGG
                     GRGHGGGRRRVQPTFDVSKFINTNPVVREPETFSPKHSFADFGLDRRITAAIAKAGLT
                     HPTPIQDEIIPHIMAGKDVIGLANTGTGKTAAFLIPLIEAVLKDGRREVLVLTPTREL
                     AIQIEAEFKRLAEGTPLRSTICVGGTNIRPQISGLKRKNHFVIGTPGRIIDLIERRAF
                     RPEGMSTVVLDEADRMLDMGFIGDMRMIVGRIPASRETLFFSATMSDDIKKLVHEFLK
                     DPVTVSVRKQDITSSVRQDVVRYQHASKFETLTKLLADPSMKRVLVFGAMKHSVEKLS
                     KELARAGIAAASIHGNKTHGQRQRALQSFKSGQSRVLVATDVAARGIHVDRVSHVINY
                     DLPATYEDYVHRIGRTGRGKEEGMALTFVEG"
     gene            11640..12149
                     /locus_tag="VFS75_02955"
     CDS             11640..12149
                     /locus_tag="VFS75_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEU4677651.1"
                     /translation="MEHLELTKLDSPRSISILFHVSMWWRIFYGSLRIVLAFVLFKLV
                     GTPFTDILRSVMGYEIAEDPTDIIFQTLYAFFETHAFTVSYFVASYLLFWGTIDVVLS
                     AFLLKHKLWAFPSSLVLMTVFIIYEVYRLFHTHSLILLWVILVDVAIIYLIYREYRIL
                     KCREDVPLP"
     gene            complement(12141..13325)
                     /locus_tag="VFS75_02960"
     CDS             complement(12141..13325)
                     /locus_tag="VFS75_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEU4677652.1"
                     /translation="MRAFAVGFLFILFIAPWSALAKTTTDELEVSGWIPYWQGSMGIK
                     DAKKHLSSLDAVYPFAFTVTIAGELRDQAGLDHSDWRNFTKAAKKKDVLVIPTVMSGD
                     GMAIYGIIANPVLRKMHVEHIVDMVKEGDFDGVDIDYEGRKADSKEYFSAFLTELKAA
                     LGDKILSCTVEPRTPPDSLYKTVPATLSYSYDYAVIGEVCDRIQIMAYDQQRADLKLN
                     TSKNGAPYIPVADVDWVRKVVELATESLPKDKIVLGVPTYGVEWAVTVYPDWFKSYSR
                     IGARNMDDMRAIARKNKVTPTRNKAGEMSFTYLPKDSSIKLSSSLRIPSDTPKGNITA
                     ARALAYANKTGNPVTVNVGWYSDARAIKDKIDLAKEFGIRGVALFKIDGEEDPKVWNY
                     LR"
     gene            13466..13765
                     /locus_tag="VFS75_02965"
     CDS             13466..13765
                     /locus_tag="VFS75_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677653.1"
                     /translation="MSKKTGKKCVKLGKVVAEIKRRIETLRNFHCDVSQRGKRFELMI
                     WLQSVRGRLLASEKRKLASIFGAYMKRFNLAIEDLAEHGRAVYCCFKYVPKRRQA"
     gene            13824..14618
                     /locus_tag="VFS75_02970"
     CDS             13824..14618
                     /locus_tag="VFS75_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677654.1"
                     /translation="MERYYVIEKEVGETPLQALSRLRRERGIANDVPLSYAGRLDPMA
                     SGKLLILVGDECKRQTSYHGLDKEYEVDVLLGVRSDSGDVLGIVESCAPAAVAAGDVE
                     AALSALRGEIELPYPHFSSKTVYGKPLHTWTLEGRLGEIEVPVKRSTVYRLDLHGLRT
                     AKTEEVVRTALSKIDALPAVTEPSKALGRDFRRDDVRASWARLPDAVWPLVSFTATAS
                     SGTYMRSLAERLAASLGTCGLALRIHRTKIGRFRRFGPWGIWTKTY"
     gene            14675..14848
                     /gene="rpmG"
                     /locus_tag="VFS75_02975"
     CDS             14675..14848
                     /gene="rpmG"
                     /locus_tag="VFS75_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4677655.1"
                     /translation="MSQDKLIKLVSVGDGKGVGKGHVYYTRKNKRKHADTKFEFKKFN
                     PIAKKHTVYKEKK"
     gene            complement(14879..16840)
                     /locus_tag="VFS75_02980"
     CDS             complement(14879..16840)
                     /locus_tag="VFS75_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU4677656.1"
                     /translation="MARTTIIGNGNLLVGIDERGFVRDFYYPYVGVANHVSGASGSYK
                     HRLGVWVNGTLAWLDDPAWTIDVERDPCGAVDAMTAQHAALGVSLRLLDVVHNEHDVF
                     FRKITLKNLGGRRTVKVFFGQEFRISESRRGDTAFYDPRVGSIIHYKGHDAFLVHARI
                     DGRPFDDYGVGLFGIENREGTYADAEDGVLSRNAIEHGSTDSVIGVAADLEGNAEATV
                     HYWITAGDSIHAVHLLHDLVLEESPERLISSAINYWRAWVGKEGRDLKPVDDRLAYLY
                     RRSLMVMRMHADNRGGIIASSDTDVLNQGRDTYGYVWPRDAAMTVEALDRAGYHDLSA
                     RLFSFFARVMEKDGYLMHKYIVDGSLGSSWHPWVRNGATELPIQEDETATPIFMLARH
                     YERAKDLEFIESLYNPFIEPAADFMCGYVDEETGLPAGSYDLWEEKFGMSTYTAASVY
                     GALMGAADLSALLGKRENVEKYRARALHVKQAILTYLYDVSSGAFVKLARREGGNLTY
                     DKTIDVSSLHGLLYFGVLSPHDPKVKSMVKHVEERLKVPTPFGGYMRFENDNYYRTGK
                     DAPPNAWCITTLWMAGYEMRVAKTRADLMKAYGMLEWVYDRATKTGILPEQIDPHTGA
                     HLSTSPLVWSQAEFVTAVDEYVRRFRALS"
BASE COUNT         3342 a         5279 c         5073 g         3203 t
ORIGIN      
        1 gattatggaa cttcccccat cccgcctcga gccacgtcca gccgaggtag gctcggacga
       61 tgagccagag ccacgcgctc ttcgtgttcg cgaggaagaa atacgatacg ggcgactcct
      121 cgatgaaata gtgcttcatg cagaatagta tgactggtaa cacttctatg atagaacacc
      181 atagacgacc atctatggta gacgtgtgta caagcggtcc gaggcaaatg cgcttcacga
      241 ccgccgcctc gcgtgctatg gtgtcgaacg gacaacacgg aggagaggta gatgaccaag
      301 atcacaggcg ctgaaaaatg gcttgaggag gcccggggga cgcttcgccg ggaccccacg
      361 tcgacgcttc cttgcctcac gaaccacgac ggctggaacg acgccgtgag cgccgtatac
      421 agcgaacccg agaactacga ggagacgctc cttagtctcg agatccgcct ggtgctccag
      481 caggcgtgtc ggaacgttca cgcgcggacg accgccaacg gataccgacc gacgcgctcc
      541 gacctcgcgg cggcgtttcg gctcggcgac atcatcgcga agacggaccc gaatatcacc
      601 ttctcgatga agcgtctcct ccacgagctc atgaccgatc tcggcttcac ggccgagcgc
      661 agtcccgcgc tcgaatggtg acgtcccacc tgcccctctc tttggagggg cttttatttg
      721 gcaaagcggc gatttggagg tacccttcct caggttcgtt cattcggagg actccatggt
      781 ccgacgcatc gcgtatctcc ttatcgcgct catgcctctt cccctctacg ggaagtgggt
      841 gcacgcgggc ataccgttca cgtacgaggc cggtgattcg taccgctaca cgcttcggga
      901 cggcacctcc tgctacgtcg gacggctcta cgcgcaccct gcggtcgtct tctacgtcat
      961 cggtccggtc gagcgtctcc gcgcgaaagg caccaccgcg cgctatgcgc gccggacgta
     1021 cgacgaagcg gttatcgacg cggaatcggg gaaacttcgg gagtacaacc acgcggagat
     1081 cgtcccccgc ccgtacggga ccggctaccc ggaggtcctt cccgacggcg acatcgactt
     1141 cccctgtaag gcgttcgtcg cggcgatccc atcccgctat gttcgcctcg gtctcacccg
     1201 cttcctcgag cgcctcgagc gagcacagaa ctcggaagag ctctttctcc aatgaaaacc
     1261 gcctcgctcg aggcggtctt ttttatgtcg ttcgcgaatg ttcacccgag attgttgtgg
     1321 accatgtgct cgagcgcgta ctgtctgtcg gcgaacacct ttttccccgg attgaagatg
     1381 cgatgcgggt cgaagatatc cttcgtgcgc tcgaagagcg cgacgacgtc gtctccgtac
     1441 atgagcggaa cgtacgagct ccgaatgagg ccgtcgttgt gctccgcggt gatggtcccg
     1501 cggtacgaca ggacgagccg gtacacttta tccattaagg cactgatgat gtcgtcggcg
     1561 tcggggcggc gcatgtccat aagcgggatg atgtggaagt tcgcgtcgcc catgtgaccc
     1621 gcgaccgtgt acgtgaggtc gtactcgtcg aggagcgcgt agaacttcgg caggaacgtc
     1681 ggaaggtcgt ccggatggac cgcgaagtcc tcgatgaacg aggcggtccg catatcggtc
     1741 ttcttctgcc ggaggaggtt gaagctctcg cgacgcatga cccaatactt ctccgcctct
     1801 tctggcccgg tcgtgacgcg cgcttcgtaa cccatctcgc gcatcgccgc gacggacgcc
     1861 tcggccttcg ccgtcgcctc ttcgtcggtg tctccgctga actcggcgag gaggatgagc
     1921 ttcggaagtc ctcccgtgag gaccgccttg aactccggcc aaaagcggag cgcgagaccg
     1981 aagatagacc cgccgagacg cttcacgatc atcgggaaga gtttgatggc gaatcggaag
     2041 gtgtggtcgt cgtacgactc gagactctcc ggagtgaacg agaggagctc gtgaatgacc
     2101 ttcggcaggg tcttcatctc cttgaggaac acgacggcga gccgcgtatg cgtgtgcggg
     2161 cggacgagcg cgaactcgat ctcggtcacg atgcccaagg ttccctgcga accgacgatg
     2221 acctgcgtga gatcgaaggt ccccgtctcc ctgtcgtata cgttccagag gtagtagcca
     2281 gacgagttct tcgtgacgtt cggcttcgac gccttgatga ggtcgtactc gctctcgcag
     2341 agttcgtgca tcttccggta tatctgaccc tcgagcgtgt cgagcttctt cttcgcctcg
     2401 agctccttca tggtgagttt ctcgaattct gcgacgcttc cgtcggagag gacgaccttc
     2461 agacggcgga catacttctc ggtcttcccg tatacgagcg tcttctcgcc gcccgaattg
     2521 ttcgcgacca tgccgccgac cgcgcagagg tccttcgacg ccgtgtacga cgggaggagg
     2581 agccccttcg cgagggtcgc cttctcgaag tcgcggtagt acatcccggg ctcgacggtg
     2641 gcgctgtcgg cgccgatctc gcgaagcgcg ttcatgtgct cggtgaattc gagaatgatc
     2701 ccctcattga gaggcccacc ggtcatgtcg gtcccggcgg agcgcgcggt caccgtgaga
     2761 cgttcgcctt cctgatttcg cttcgctacg tattcgacga gcttctcgac gtccgtcgca
     2821 gatctcgggg cgacggccaa agacggtcga accgtgaaga tgctcgcgtc cttgctcaag
     2881 cgctcgagcg tcgccgcatc gtcccgtacc tcaccctcga tgagcgccgc tatctcctct
     2941 ttggtttttt gttgcatacg aatcggcgcg cgagcgcgct tttaagaagt ataccaagcg
     3001 ctcgcataga ccgcggccga tatgtcccca ggcaaccgtc acgagaactt catggcgcga
     3061 gtgctatcgt ttccctgccg gtgcatccat cccatgaaac ttcgcgatat cccaaagatg
     3121 gaggtccaac ggcatatgtc gccggagatc caatacatgc agtcgcacgc gcacctctac
     3181 gtcgaccgca tcatcaagaa cactcgttcg ttcccgcaga tatcgtcgat cgacgacctg
     3241 ccgccgggcg cgatacggac gctccgcggc gggaggatgt cggcgctcta cagcgtcgcg
     3301 atcgagaacg agctcatcgt catcaaaatg cgccgagacg gtatcgaagc ggagtgcgag
     3361 gcgctcgagg cgtggcgtcg cgtgaaggtt cccgtcccga cggtcctcaa aatggggacg
     3421 ctcgccgaca cgaagacgat gaccggtgtg aagtatctcc ttctcgactt cgtgctcgac
     3481 cggagcggcg atcctgcgcc gaccggcgcg gactacctcg aacgtcaccc gagcgcggcg
     3541 caccgcgttg gtcgtctcct cgggaggacg ctcgcgcgca tgcaccgcgc gaagaccgac
     3601 cgcaccttcg gcgagtacgc ggacatgggc cactcgaacc ggtcaccgta ccgctcttgg
     3661 aacaagtacc tgctcgggta cctcgatgcg taccggccgt acctcgtgtc gctcggtctc
     3721 acgcgaaagc tcgtgaagcg gctcgagacg accatcagag atgcggagtt cggcaccgtg
     3781 gggacgtacg tgcacggcga ctatagtctc cggaacgtca tgatcgactc gaggaaaccc
     3841 ctccgtgcgg tcgtgttcga cccgaacccg gtgatcggcg acccgtcgtg ggacatcgcc
     3901 atcctcatga acaaccggga ctacgcgctc cgtcgatacg cgtgggaacc gcaggaccaa
     3961 gcgtatcgga agatcgcgaa gcgagaggaa cgcttcctcg agggtttctg ggcgtcgtac
     4021 cggaacgcga gccgtgagcg cgttcccctt tcccacattc gcatcgcgca gctcctccag
     4081 acactcctcc acctcgcgac ggaggaacgg aaggagtacg cgcgaccgga ggagaaggcc
     4141 gtcgagatac gctcgcgtcg cgagacgctc ttcgagctca tcggaacgct taccatgaat
     4201 tcgtaaccaa ggagaagaac catgcgagtc tatttcgttc gacacgggga gcgcgcggac
     4261 aattccgact cgatcttcgc gagccgcgac atcccgatca ccgagctcgg caagcggcag
     4321 gcaggactcc tcgccgagcg cttcgcctcc atcccgatcg acgccatcta cgcgagtccg
     4381 tacgtgcgga cgcgcgagac cgccgagatc atcaacgcgg tgaagaagac cggcattcga
     4441 tacgatgcac gcatcgaaga gctcggccgc gaggaagagc tcgcgaacgt ctttcggcgc
     4501 gcgaactcgt tcgtctcgtt cctcgagaac gaatgtgtcg aggagcggat actcgtctcg
     4561 gcccatgcgg gcatcatcaa gatgatcgtc ttcgcgatgg tgttcggcga gctccacctc
     4621 ggcggcgcgt tcaaagagtt cgaccgctcg ttccacatgg acttcaccgg cataacgtac
     4681 tgcacgcggg aaaagccgaa tccatggcac atcgtgtcat ggaacgacct cggccacctt
     4741 cctccgccgg tgtgaccacg tccgttcgcc ccttccgcgg aaggggcttt tcattcgccg
     4801 aacctcttct ttccgatacg gtaggcgagg taatggagcg ttcccatcgc gagagccgtc
     4861 gcgaaaagga cgacggcgag cttcacgcgc gcgtcggtga gcgactccgc gcttcgcgcc
     4921 gcgatgcgga cgacgccata atagacgcac cccaggatga gaacggcgaa ggcgccgacg
     4981 agctgaaacg gcgggagcga taccttccgc gctccgcaga ggatctgtgc ggcgatacgc
     5041 gtcccataga cgaacttcga gtagaagagg acgacgagct cccttccccg gaccacgcga
     5101 gagagacgcc gaaccttatc ggagcgactt tcgatgaaac ggcgctcgat gaaccgcgga
     5161 agcatcccgc gcccgaccgc gtaccaaaag ctatcgttca cgatggcggc gccgaccatg
     5221 acccagatga tagacgacgc gcttagcgca cccgagagcg agagatagaa cgcgggaaag
     5281 aggatgatgc caccgccgac gaggaggctc aggaagatga tcgcgtacgg gtgcgcggcg
     5341 aagacggcga cgatgccgtc cgggatgccg gccgcgatgg ccgacgcgta gagcgactct
     5401 ccgtgcatag cgaatcgatg ataccgggga gcggattaaa aacgggtgaa cgcgaaaaag
     5461 gaccctcgcg ggtcccgttt cgtcatcgcc ctcgcttact cctcgagggg cgttcgcgac
     5521 accgcctgcg cgctcgtccc gcgcatcact cggtcgaaga tgatggagct cgactcgccg
     5581 aatgggtcgt ggtagttcgc gagcttgagg taacacgaga tgcccttgta gagcgtcggt
     5641 ccctcatggt cgagaacctt cttcccgttc atccatgcga cgaagtaccc atcgtcgccc
     5701 gaggaccact tcacgtggta gacgaggtcg taccactgat cccggaccac cgggccgagg
     5761 tcgaccttgt actcctctgg attcgagacg tcaccgtaga aaccgtgcat gcggagcccg
     5821 gtcggctggg agaggaaatg gaagttcgcc tgtccgccgc tcgcgttgtg gtggaagtcc
     5881 atgacggcgc ctccttcctt cgggacgacg tagctatcgg gaaagaggac cgacatcgcc
     5941 caccactcct cctggccctc gttgcagtaa tcgggcgacg tcccgagaga gaggtcatcg
     6001 cgctcccagt acccgctccc atgcacctcg tcgtctcccg gcatcgtcgt gagcatgact
     6061 gcgcgctccc cgtttcgagc cgtaccgacg aacgaactcc gatggccgtc gggttccact
     6121 ttcgactgct cgtacatgcc gcagtacgaa tcctcgaaat cgcacttcca gaggacgacg
     6181 ccctcgtccg cgggaacgtc taccgatggg gtcgtctcct cggtcggcgc ggttgtcggc
     6241 gccgggtcgg tcgatgaacc atcgtctccc ttcggctctg gcgttggtgt cggagaaggg
     6301 gtcggcgcgg gtgtcggcgt tggagaaggg gtcggcgtct ccgctttccc gtcgccgtcc
     6361 ctctcctccg taggcgcgtt cggcgcacta gtgccgctat gcggcgacgc gtgcttcttg
     6421 accactttca ccggcgaggg cttcgtcgcc tttcgtacga cgaccgtcga tgccgcccta
     6481 acgggcgtcg gtgccttcac cgcggtcggc gcatcgaaca cgtacgcgat gcgcgagagc
     6541 ggtccgaagt aaccagagtt cggtgtgacg ccgtgcgcgg attgccagag agatacggcg
     6601 gccttcgtct gcgacccgaa gtaacccgtc gcgcctgccg ggatgacgat gccatggtcg
     6661 agaagcatcc gctggaggca cgctaccggc gccccagcgg acccgatcgc gaggtccgtc
     6721 gtgaagacgc agcgagccgc ggcgtccgcg acgcctgcga agaggaacgc ggcgagcgcg
     6781 atggcgaacg acactgataa ggaacgtttc aaacgaatgg tcataatggc tatggaatga
     6841 acgtgtctcg taatgcggcg ccactctatc attttcacca cctgcccgct cgccgtactt
     6901 cacgaaaact ccattcactt ctccgtatac tcgctttgta gccgccgttt acgactttta
     6961 cacactaagc atcatattgt tcaatgagga gcgagcatct tcgagagaga acgttaacca
     7021 tcgcgcgcgc atgcgcgatg acgatcgtcg ttatcctggt ctcgttccat accgcatcgc
     7081 gcgccgatgc cgcgactgac ccgaatctcg ttccgaaccc ttccttcgaa acagccggcg
     7141 ggaacgggct ccccgcgaac tggggtaagg gcggctatgg tacgaacgat cgtgcgttca
     7201 cgtacccggt ctccggctac ggaggcggca aggccgcgcg cgtcgaggtg aagaactaca
     7261 cgtcgggcga cgcgaagtgg tacttcgcgc cggtctcggt cacgccgctt caggcgctcc
     7321 tcatcaccga cgagtaccga agcgacgcga cgagctacgt caccgtgcag atcgtgcaca
     7381 ccgacggtcg catcacatac gcggacctcg ggaaactcga cccttcgagc acgtggacga
     7441 agttcaccgg ctactacacg ccgcccgcgg acacagtgaa ggtatccgta ttccacgtta
     7501 tcaagagcac ggggttcctc gagaccgacg acttcttcgt ggggacgacc gggccgaacc
     7561 tcgtgccgaa cccaacgctt tcgacgaaga ccgatgcggg actgccggcc aactggcaaa
     7621 agggtggcta cggagcgaac gaccgctcgt tcacgtaccc ggtcaccggg tacgatgacg
     7681 ggagcgccgt gcgcgtcgac atcggctcct actcctcggg cgacgcgaag tggtacttcg
     7741 cctcggtccc cgtgaagccg ctccagcccc ttaagttcga ggaccactac cgaagcgacc
     7801 gtgaaagtta cgtgacgata caggtgacgc acgatgacgg ttcgaacacc tacgtcgatc
     7861 tcgcgaaact cgcgcctgcc tcgtcttgga cgtcgtacac gggctactac acgccgcccg
     7921 cggacgccag ctccctaacc gtcttccacc tcatcaaggg cgtcggcttc ctcgagacgg
     7981 acgggtactt cctcgcgcag gtgctctccg acccgaaccg cttcgatagg gggtacgtat
     8041 ccctcatgtt cgatgacggc gtcgaaacga cgtacaagaa cgccttcccc atcctcgacg
     8101 cggcgaactt caaagtggac acgttcatca tcaccgaccg gctgaaggac ggtacgtacc
     8161 cggggtacgt gaaggcggct gacgtcatcg cgatgcagaa cgcgggccac gagatcggcg
     8221 cgcacacgcg ctcgcaccct gatctcacga cgctctcgcc agcggacgcg aaggcggaga
     8281 tcgaaggttc gagaaacgac ctcctcgcga tcggcgcgaa gcccgtgaac ttcttcgcct
     8341 acccgtacgg cgcgtacaac acgcccgtca tagacgacgt gaaggcggcg ggattcctcg
     8401 gcgcgcgctc atcggacggc gggtacaacc tgaagacggg taaccaattc gcgcttcggc
     8461 gcatctccat ggaccggacg acgacgctcg ccgacgtgaa gcagtacgtc gagacggcga
     8521 aggaggataa gacgtgggtc atcttccttt tccacgacgt cgatgacagc gggagcgagt
     8581 acgcggtatc acccgcgctc ttcaaagaga tcgtcgacta cttgcagtcc gccggcgtga
     8641 cacccatcac catgtcgaag ggcatatcgc tcatgaacca atagcgttca tcccgagttc
     8701 accgagattt acgtatcatg gagtcatgac cttactcgcc taatcacaca tgcaccatga
     8761 acgctaacga gttcttgaag aaacagcatc gagacgtaca ggcgctctac gaggagttca
     8821 aggcggcgga cggcgacgcg aaagacgaga tcggcaagaa gctcctcact gctctcgtcg
     8881 tccatgacga catcgaggac gagctctact accccgaact cgagaaggtc ggactcaagg
     8941 atagcgtcga agagtaccgt gccgagcacg tcgcgaccga gacgaacatc ggtcgcctca
     9001 agacgatgga ccacgaggac gacgagttcg aaccgaccat gaaggcgatg atggagggcg
     9061 tgaaccacca catcgaggag gaggaaacgc agggcatgcc gaagttcgag gcgctcgtcg
     9121 accccggcgt ccttgcggac ctcggcgcga agctcgacgc gcgttcgaaa gagctccatg
     9181 aatcgacact gaagcgtctt tgggcgaccc tcacctaacc atatgaaaaa aacactcgtc
     9241 aaaaggtcac ggcggaagtt ctccgcgcgt caatcattcc cgttctcccg agacctcgcg
     9301 cacaaccacg tgtggccgag gcgatgcggg ttcgcatcac ttcgagaggt ccgcgagctt
     9361 tgagtcgtac tcctccttcc ctatctctcc cttcgcgtat cgctctttga ggatgtcgat
     9421 ggcgttcgag cctcgtccac agtgatgacc gtcatagtgg tgcccgcgag acgcgcgaac
     9481 gagccacacg atgcccgcga cgacgagcgc ccagaagagg accgtaccga tcatgccgct
     9541 aagttcccat ccgtacccat gcccatagta cccatagttc atcatagtag ttaggttaaa
     9601 ccgctaaaaa ctacctctat tgtatgcgcg cctaaaatga atgtcgagtg aacggaacgt
     9661 tcgcgctacg cgtgcgtatc atcgcgtcgc ggcatcgcta tgtgcgggat acgcatgacg
     9721 aggacgccga tcaaggttga gaagatgccg atgacgtgga agccgaaacc gaaacacatg
     9781 ccgatcgctg cggagtacca aaggctcgcg gcggtcgtga gattatggac cgccttcccc
     9841 tctttgagga tgagcccgcc gccgaggaaa ccgacaccca tgacgacctg cgcggcgatg
     9901 cgcgccgtaa agtcgggttc gatatgcgca gagatgaacg tgaagagcat cgcgcccatg
     9961 atgacgaggg attgcgtgct tatgcccgcg tcctttccgc gtgactcgcg ctcgagaccg
    10021 atcaagaacc cggcgagaag cgaaagcgcg aggttcagca tgaagagaag ttcgatctcg
    10081 ggaaggagcg acgtcatata tatggagaat gatacgccag cgcgaaagcg attcctaggg
    10141 atgcgactgc acatcttcgc cgtttgacat cggcgccgcg gtcgcgtacc atcatgctac
    10201 ctgaagattc tcgtgtaggg ctcttgggta cgaaggctcc cccttcggtt tacttaccta
    10261 cacagcccat aagacacaca acgtatatgc acaatgcaca ccggcaaggc cggagctcgt
    10321 cttcgcgttt cgcgaggcag ggcggccgag gccgaaacac attccacgcg agcggtcgtc
    10381 gttcgggtgg ccgacagacg aaccatcgcg gcggcggagg acgcggacat ggaggcggaa
    10441 ggcgtcgagt tcagccgacc ttcgacgtat cgaagttcat aaacacgaac ccggtcgtcc
    10501 gcgaacccga gacgttctcg ccgaagcaca gcttcgccga cttcggactc gaccgccgca
    10561 tcaccgcggc gatcgcgaag gccggcctca cgcacccgac ccccatccag gacgagatca
    10621 tcccgcacat catggcgggc aaggacgtca tcggtctcgc gaacacggga accggcaaga
    10681 cggccgcctt cctcattccc ctcatcgagg cggtgctcaa ggacggacga cgtgaggtgc
    10741 tcgtcctcac gccgacccgt gagctcgcca tccagataga ggcggagttc aagcggctcg
    10801 cggagggtac gcctctacgc tcgaccattt gcgtcggcgg tacgaacatc cgtccgcaga
    10861 tatcgggcct caaacgaaag aaccacttcg tcatcggtac gccgggccgc atcatcgacc
    10921 tcatcgagcg ccgtgcgttc cgccccgagg gcatgtctac cgtcgtcctc gacgaggccg
    10981 accgcatgct cgacatgggg ttcatcggag atatgcggat gatcgtcggc cgtatccccg
    11041 catcgcgtga gacgctcttc ttctcggcga ccatgtccga cgacatcaag aagctcgttc
    11101 acgagttcct caaggacccc gtcaccgtct ccgtgcgcaa gcaggacatc acgtcgagcg
    11161 ttcgtcagga cgtcgtcagg taccagcatg cgtcgaagtt cgagacgctc acgaagctcc
    11221 tcgccgaccc gtcgatgaag cgcgttctcg tcttcggtgc gatgaagcac agcgtcgaga
    11281 aactctcgaa ggagctcgcg cgggcgggca tcgccgcggc gtccattcac gggaataaga
    11341 cccatggcca gcgtcagcgc gcgctccaga gcttcaagtc gggacagagc cgcgtgctcg
    11401 tcgcgaccga cgtcgcggca cgcggcattc atgtcgaccg cgtctcgcac gtcatcaatt
    11461 acgacctccc cgcgacgtac gaggactacg tccacaggat cggccgcacg ggccgaggca
    11521 aagaggaggg catggcgctc accttcgtcg agggataagg acgatagcga taaacgaaaa
    11581 cgggccgcgc acgcggcccg ttttcgtttc gcgtcgaccc tctgctatac tcgtctcata
    11641 tggagcacct cgaactcacg aaactcgact cgccgaggag catatccatc cttttccacg
    11701 tgagcatgtg gtggcgtatc ttctacgggt cgctccgcat cgtcctcgcc ttcgtcctct
    11761 tcaagctcgt cggaacgccg ttcaccgaca tcctccgctc ggtcatgggc tatgagatag
    11821 cggaagaccc gaccgatatc atcttccaga cgctctacgc gttcttcgag acgcatgcgt
    11881 tcacggtctc ctatttcgtc gcatcgtacc tcctcttttg gggaacgatc gacgtcgtgc
    11941 tctcggcgtt cctcctcaaa cacaagctct gggcgttccc ttcgagcctc gttctcatga
    12001 cggtctttat catctatgag gtctaccgtc tcttccacac gcactccctc atcctcctct
    12061 gggtcattct cgtcgatgtc gccatcatct acctcatcta tcgcgagtat cggatactca
    12121 agtgtcgaga ggacgtaccg ctaccgtagg tagttccaaa ccttggggtc ctcctctccg
    12181 tctatcttga agagagcgac gcctcggata ccgaactcct tcgcgaggtc gatcttgtcc
    12241 ttaatggcac gggcatcgga gtaccacccg acgttcaccg tgacggggtt tccggttttg
    12301 ttcgcgtacg cgagggcgcg agcggcggtg atattaccct tcggcgtatc ggatggtatc
    12361 cgaagcgaac tcgaaagctt gatcgacgaa tccttcggga ggtacgtgaa gctcatttct
    12421 cccgccttgt tccgcgtcgg cgtcactttg ttctttcgcg ctatggcgcg catgtcgtcc
    12481 atattgcgag cgccgatacg cgagtagctc ttgaaccagt cggggtaaac cgtgaccgcc
    12541 cactcgactc cgtacgtcgg cacgccgagc actatcttgt ccttggggag actctccgtc
    12601 gcgagttcga cgaccttccg cacccagtcg acgtcagcga ccggtatgta cggagcgccg
    12661 ttcttcgacg tattgagctt caggtccgcg cgttgctggt cgtacgccat gatctgtatg
    12721 cgatcgcaga cctcgccgat gaccgcgtag tcgtacgagt acgagagagt cgcgggtacg
    12781 gtcttataga gcgaatcggg cggcgtacgc ggctcaacgg tgcacgagag tatcttgtcg
    12841 ccgagcgctg ctttaagctc cgtaaggaac gcggagaagt actctttcga gtcggccttc
    12901 cgtccctcgt agtcgatgtc gacgccgtcg aaatcgccct ccttcaccat atcgacgatg
    12961 tgctcgacgt gcatcttccg aaggaccggg ttcgcgatga tgccgtagat ggccataccg
    13021 tctcccgaca tgacggtcgg gatgacgagt acgtcctttt tcttcgccgc cttcgtgaag
    13081 tttctccaat cgctgtgatc gagtccggcc tggtcgcgaa gctcgccggc gatggtaacc
    13141 gtgaacgcga acggatagac ggcgtcgagg ctcgagaggt gctttttcgc gtctttaatg
    13201 cccatgctgc cctgccagta cggtatccat ccggatacct cgagctcgtc ggtcgtcgtc
    13261 ttcgcgagcg cgctccacgg cgcgatgaag aggatgaaca aaaacccgac ggcgaaagcg
    13321 cgcatgttcg atgtatgcat gttcaggatg acgaagaccg gcttccctcc ttactcatgg
    13381 gctcttcata tccccgtttg tagcgcgcgc gccctggtgc tacagtagta ccggaaatcc
    13441 gtgcaatctt cgagaggagg cttccatgag caaaaagacc ggaaagaagt gtgtgaagct
    13501 cggcaaagta gtggcggaga tcaagcggcg catcgagacc cttcggaact tccactgcga
    13561 cgtctcccag cggggcaagc gattcgagct catgatctgg cttcagagcg tgcgcgggcg
    13621 gctcctcgcc tcggagaagc ggaagctcgc gagcatcttc ggtgcttaca tgaagcggtt
    13681 caacctcgcg atcgaggacc tcgccgaaca cgggagggcc gtctactgtt gcttcaagta
    13741 cgtcccgaag aggcgacaag cgtaggacga ggaaggggtg gccaaggccg ccccgttttt
    13801 tatgcgcgtg ctataacgta tcgatggaac gctactatgt catcgaaaag gaggtcggcg
    13861 agacgccgct tcaggcgctc tcgcgcctcc gccgcgagcg cggcatcgcg aacgacgtac
    13921 ccctttcgta cgcggggcgg ctcgacccca tggcgtcggg caagcttctc atacttgtcg
    13981 gcgatgagtg caagcgccag acgtcgtacc acggtctcga caaggagtac gaggtcgacg
    14041 tcctccttgg cgtacgctcg gactccggcg atgttctcgg tatcgtcgaa tcgtgcgctc
    14101 ccgctgcggt cgcggcgggc gatgtcgagg cggcgcttag cgcacttcgt ggagagatcg
    14161 agcttcccta cccgcatttc tcatcgaaga cggtctacgg gaagccgctt cacacgtgga
    14221 cgctcgaggg ccgtctcggc gagatagagg tccccgtgaa gcgctcgacc gtataccgcc
    14281 tcgaccttca cggcctccgc acggcgaaaa ccgaggaggt cgtgcgaacg gcgctctcga
    14341 agatagacgc gctccccgcg gtcaccgaac catcgaaggc gctcgggagg gattttcgac
    14401 gcgacgatgt ccgagcctcg tgggcgaggc taccggacgc ggtgtggcct ctcgtgtctt
    14461 tcacggcgac cgcatcctcg gggacgtaca tgcgttccct cgccgagagg ctcgccgcct
    14521 cactcggcac ctgcgggctc gcgctacgca tacatcggac gaaaataggc agatttcggc
    14581 gtttcggccc gtggggaatc tggacgaaga cgtactgacg aggcgtccag aatccgttga
    14641 cggatagcgc tccttctggt actatcccgg gactatgtca caggataaac tcatcaaact
    14701 cgtctccgtg ggcgacggca agggcgtcgg aaagggccac gtctactaca cgcggaagaa
    14761 caagcgaaag cacgccgata cgaagttcga gttcaagaag ttcaacccca tcgcgaagaa
    14821 gcacacggtg tacaaagaga agaaatagag aagcggccgg ggatcccccg gccgtttttc
    14881 atgagagcgc tcggaagcgc cgcacgtact catcgaccgc ggtcacgaat tccgcttggc
    14941 tccagacgag aggcgaggtc gagagatggg cgccggtgtg cggatcgatc tgctcgggaa
    15001 ggataccggt cttcgtcgca cggtcgtaca cccactcgag catgccgtac gccttcatga
    15061 ggtcggcgcg cgtcttcgcg acccgcatct cgtagcccgc catccagagg gtcgtgatgc
    15121 accaggcgtt cggcggtgcg tccttccccg ttcggtagta gttgtcgttc tcgaagcgca
    15181 tatagccgcc gaacggcgtc gggaccttga gccgctcctc gacgtgcttc accatgctct
    15241 tcaccttcgg gtcatgggga gagagtacgc cgaagtagag aagtccgtgg agactcgaga
    15301 cgtctatcgt cttgtcgtac gtgaggttcc ctccctcgcg acgcgcgagc ttcacgaacg
    15361 cgccggaaga gacgtcgtag aggtacgtga gtatcgcctg cttcacatgg agggcgcgtg
    15421 ctcgatactt ctcgacgttt tcgcgcttcc cgaggagcgc ggagaggtcc gcggcaccca
    15481 tgagcgctcc gtagaccgat gcggccgtat acgtcgacat gccgaacttc tcctcccaga
    15541 ggtcatacga gcccgcgggg agtccggtct cctcgtcgac gtacccgcac atgaaatcgg
    15601 ccgcgggttc gatgaacgga ttgtagagtg actcgatgaa ctcgagatcc ttcgcgcgct
    15661 cgtagtggcg cgcaagcatg aaaatgggcg tcgcggtctc gtcctcttgt atgggaagtt
    15721 ccgtcgcgcc gtttcgaacc cagggatgcc atgaacttcc gagcgaaccg tcgacgatgt
    15781 acttatgcat gaggtagccg tccttctcca tgacgcgtgc gaagaaggag aagaggcgag
    15841 ccgagaggtc atggtacccc gcccggtcga gggcctcgac cgtcatcgcc gcgtcgcgcg
    15901 gccacacgta cccgtacgtg tcgcggccct ggttgaggac atccgtatca gatgacgcga
    15961 tgatgccgcc gcggttgtcc gcgtgcatac gcatgaccat gagcgagcgg cggtagaggt
    16021 acgcgaggcg atcatccacg ggtttgaggt cgcgtccctc cttccccacc caggcccgcc
    16081 agtagttgat ggccgaggaa atgaggcgct cgggagactc ctcgaggacg aggtcgtgga
    16141 gaagatgaac ggcgtgtatc gagtcgcccg ccgttatcca gtagtggacg gtcgcctcgg
    16201 cgttcccctc gaggtcggcg gcgacgccga tgacggagtc ggtcgatccg tgctcgatcg
    16261 cgtttcgaga gaggacaccg tcctcggcgt cggcgtacgt gccctcccga ttctcgatgc
    16321 cgaagagccc cacgccgtag tcatcgaacg gcctcccatc gatgcgcgca tgaacgagga
    16381 acgcgtcgtg tcctttgtaa tggatgatag agccgacgcg cgggtcgtag aaggcggtgt
    16441 cgccgcggcg ggactcggag atgcgaaact cctgtccgaa gaagaccttc accgtgcggc
    16501 gtcctccgag gttcttgagc gtgatctttc gaaagaagac gtcatgctcg ttatgaacga
    16561 cgtcaaggag tctaagcgag acgccgagcg cggcatgttg tgcggtcatg gcgtcgaccg
    16621 cgccgcatgg gtcgcgctcg acgtcgatcg tccatgcggg atcgtcgagc cacgcgagcg
    16681 tgccattcac ccacacgccg agccggtgct tatacgaacc cgaagcgccg cttacatggt
    16741 tcgcgacacc gacgtacggg tagtagaagt cgcgcacgaa gccgcgctcg tcgatgccga
    16801 cgaggaggtt cccattgccg atgatcgtcg tcctcgccat gggggatcag acgtcgtata
    16861 ccttgagtcg tcggacgacg agggtccgcg cgctctt
//
LOCUS       DASTSP010000067        12265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_584187, whole genome shotgun sequence.
ACCESSION   DASTSP010000067 DASTSP010000000
VERSION     DASTSP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584187"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..907)
                     /locus_tag="VFS75_02985"
     CDS             complement(41..907)
                     /locus_tag="VFS75_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU4677657.1"
                     /translation="MASLRIPFFAALISLACLSFVASASFDEERGVPLLHVDFLDVGQ
                     GDATFIESPSGTQVLIDGGRDGSVLRDLSSVMGFFDRDIDMVVATHPDLDHIGGLAGV
                     LDRYRVRTIVLTENVKSTPEFEEFLRAVRDEGATVVYARRGDVFDLGAGPAGTTTLSV
                     LFPDRDPSGLESNASSIVTELRYGTTGYVMTGDSPREIEEYLVSLGTSTLRSDVLKVG
                     HHGSRTSSSPIFLDAVHPTYAIISAGKNNSYGHPHKEVVDLLDARGITRKNTADVGSI
                     FSSSDGVAVSVR"
     gene            997..1590
                     /locus_tag="VFS75_02990"
     CDS             997..1590
                     /locus_tag="VFS75_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEU4677658.1"
                     /translation="MHTYTAKTFDLPALDGLSEKQIKVHLGLYEGYVKHVNLLREQIA
                     ELTALDKEKYAYAIAETRRRLGFEFDGMRMHEYYFEQFEGEKGSDPESALAKAAAEKY
                     GSGENFIAHIKEVAGTRGIGWVVVYADPTGGTLHTAWVGDHELGQLSGLPIILAMDMW
                     EHAFMVDYVPAEKKNYIEAFLKNVNWRVVEDRYAKLG"
     gene            1601..1789
                     /locus_tag="VFS75_02995"
     CDS             1601..1789
                     /locus_tag="VFS75_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677659.1"
                     /translation="MKPSDPRTTRTILWVLFVLLIVLGASHPQGLTFPLLIGAFLVFY
                     IVIGNFVYDPLVAIFPKG"
     gene            complement(1790..3379)
                     /locus_tag="VFS75_03000"
     CDS             complement(1790..3379)
                     /locus_tag="VFS75_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU4677660.1"
                     /translation="METRVTSGTHPNGLLFFGVALAFTSGIAARSFFAFDRALLLTLA
                     LTALALLFASSIERSRTLRVAALLCFALALGALRLDYAERAPSELSSYIGEEVTVTGR
                     VVREPEVGERTTQFVVRPSGMDELVLASVSREGGIPVPEYGDAVVVEGTMRAPEAFST
                     NGGRTFDYPGYLKARGIEVTVPFATVAVLARDTSSPLARLYAGKHRFMSAVEAALPMP
                     ESGLAEGLLLGVRRALGDDLERMFRETGVVHIVVLSGYNIMVVVAALMHVLTFFLFPR
                     TRTIVGIAAITCFALLVGPSATVVRASIMASLLILAHATGRTYAALRALVIAAILMLI
                     FEPYLLVHDPGFQLSFLATLGLIALSTPVEARLRFLPERFGLRGYATATIATQAFVLP
                     LLLYETGILSLVSVLVNVLVLPVVPLAMLLTFVTGIAGLIATPLGTLVGFFAHLTLAY
                     VIIVVRAFASLPFSSVSLPAFPFTVTLALYGVTALGLFASRRPKVGRSVENEYAGWTI
                     VEEHELARAANEDAASTFPFR"
     gene            complement(3364..4062)
                     /gene="rnhA"
                     /locus_tag="VFS75_03005"
     CDS             complement(3364..4062)
                     /gene="rnhA"
                     /locus_tag="VFS75_03005"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEU4677661.1"
                     /translation="MKEVTIFCDGSSIGNPGPGGWGASIETREGKEQSVVELGGFAKD
                     TTNNRMELTAALKALSHIPARATVTIRTDSSYVINGITKWVKGWERNGWQTKEKRDVL
                     NRDLWEKLKRANDEHDVTWEHVRGHAGHAGNERVDVIANGFARGERVRLFRGSAKEYR
                     EFLKGMPKARAVSSSSSRKGTAYAYVSRVDGVVMTHKTWAECERRVKGKHAKFKKAFS
                     KEEADELVATWKRE"
     gene            complement(4211..5935)
                     /gene="recJ"
                     /locus_tag="VFS75_03010"
     CDS             complement(4211..5935)
                     /gene="recJ"
                     /locus_tag="VFS75_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU4677662.1"
                     /translation="MNAPRRTYELLPKAPDTLDDALNGYHPIQRQLLFNRGLNDAESI
                     RRFLEPDFESDLHDPFLLTDMEKAATRVLRAVHENEHIVIYTDYDCDGIPGGVLLHDF
                     LTEIGYDNFENYIPHRHDEGYGFNPDAVPAFKERGASLIITVDCGITDHEAVERANDL
                     GIDVIVTDHHEPAETLPPAYAVVNPKRDDAYPFSGICGTAVAWKLVGAVLSIGRKDGT
                     ITLKEGMEKWWLDLVGLATVADMVPLQDESRVLARYGLSVLRKSRRPGLQQLLRKAGA
                     NQRFLTEDDIGFTIAPRINAASRMDDPEDAFHMLRTRDEADAGARVYHLEKLNNERKG
                     VVGSMVKEAKRRIEELIELPQVIVMGNPEWRPSLVGLVANSLSEEFGRPAFLWGRDGK
                     GIIKGSCRSAGGVSIVRLMELAKDAFITFGGHHASGGFSVSDERIHTLPEALHAAYAE
                     HGSDVLVGDVANAVDAELSLDDIDDRLLRALDALAPYGVGNPKPLFKIRSVMPRAVEV
                     FGKTKNHTKLRLETAHGRIDAISFFALPDSFTKVPKEGETFDLIAHVERSYFMGRLET
                     RLRLVDIV"
     gene            5982..6371
                     /locus_tag="VFS75_03015"
     CDS             5982..6371
                     /locus_tag="VFS75_03015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU4677663.1"
                     /translation="MNDIFDKILRKEIPAQFVYEDDVCVVVMDKYPVVPGQVLVIPRD
                     CIDYFFEMDDVKYHHCLSIAKRMAAALDVTYETERTCMIIEGFEVPHAHLKLYPVTRR
                     EFTPHGGKEVDDATLEKEAEKIKANLR"
     gene            complement(6399..6872)
                     /locus_tag="VFS75_03020"
     CDS             complement(6399..6872)
                     /locus_tag="VFS75_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU4677664.1"
                     /translation="MKKQLTLAYLIDADRICLALKKRGFGEGNWNGFGGKVEDGESIA
                     EATVRELKEESTVGVSPDDLEPASVIEFHFKDGKHLEVFSFFVRRWDGEPRETEEMRP
                     AWFSFSEIPLDKMWADDEHWLPRALRGERLRGVVRFSGDGRTIESMEWTAVAMLP"
     gene            complement(6878..8014)
                     /gene="pgk"
                     /locus_tag="VFS75_03025"
     CDS             complement(6878..8014)
                     /gene="pgk"
                     /locus_tag="VFS75_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU4677665.1"
                     /translation="MSTKPRSVEALTDIFGMRFLVRASLDVPLEGGIAADHFRVRRAI
                     PTFVHLLDRGARVIILTHVGRDPENSTESLVSLLAKHIPVRYVPAVTGDEVYGAVAHM
                     KEGTALLLENLRRDPRETANDPEFARELASYGDFYVNDAFAVSHREHASIVGIPKFLP
                     SFAGIVFMEELNELTKVVAPASPSLFVLGGAKYETKEPLIEEYSKTYTRTFIGGALAN
                     DFLKGKGYEVGTSVVSPVDLTGNELLTRENLKLPIDVVVEKGGTTRTAAVDEVAKDEK
                     IVDVGLASLEMIEPYIAEAETILWNGPLGYYEGGFDAATKRCADLIASSSAYSVVGGG
                     DTVAAIESMGLQDKFGFLSTAGGAMLEFLEKRTLPGIEALPKES"
     gene            complement(8011..8772)
                     /gene="tpiA"
                     /locus_tag="VFS75_03030"
     CDS             complement(8011..8772)
                     /gene="tpiA"
                     /locus_tag="VFS75_03030"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU4677666.1"
                     /translation="MKTSPLVIGNWKMNPTSVSLAKKLASDLKKRLARMTGTDVVIAP
                     PYPYLETLRAVRSESKSFALGAQNVHDEKQGAHTGEVSIPMLESFDVTHVIVGHSERR
                     AAGETDTEVNRKLLSVVKSGLTAVLCVGETKRDHAGHYLTTVEGQIRAALSSLGKAKL
                     GAVVIAYEPVWAIGTGENATAADVHEMRLFIEKVLADIYGRTYATRVRILYGGSVNAK
                     NARELFVEGTVRGFLVGGASLKAGEFIDIINATRT"
     gene            complement(8835..10142)
                     /locus_tag="VFS75_03035"
     CDS             complement(8835..10142)
                     /locus_tag="VFS75_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677667.1"
                     /translation="MNNERVGIQNTRGAALLLFVVFFLAISLTLVLSIGGGVYDDLAR
                     FKDMKLGKESYYAADAAIEDAVYRYRAGMNYSATESFTVGTADVAVTRTINGKLYDFL
                     AVGDDDSRIRRSSAQLFYGDGASFNYGLQSGNGGIKMDNTSHVFGNIFSNGSIVGTNF
                     NFVWGDAISAGPTGRANGVHVVGNAWAHTITSSIIGLDAYYQTMTGTVVFGSTHPGSA
                     DQATATMPIDDDKVAEWEAAAEAGGVIAATDPRCSSGTYLIQSDVTIGPVKINCNVHF
                     DKSGTDVTLAGPVWVVGNIDDTNATLRVDASLSGMSVPVIADDPANRTTGSKIDLGNS
                     PTFVGSGAGSYVLLLSQNRSYEDGGGETAIEVSNSANGDLLLYAGHGEALLENNVSVN
                     ELTAYHIHLKNSAEVTYESGLANLLFTSGPGGGYSITSWQESE"
     gene            complement(10135..10641)
                     /locus_tag="VFS75_03040"
     CDS             complement(10135..10641)
                     /locus_tag="VFS75_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4677668.1"
                     /translation="MTHVSQNKRGFTLIEMLVYVSVMLLIAVGLITTFHSLDTVLLKN
                     RDDRALTESASVALERMGSALRGATIVDTGASTLGTSPGVLTLIAGATTTTFSLSGGR
                     VILSENGEEVGPLTGSDVTVDDLRFTHYVGTATELVRVALTLTRVGSVSTSTRTFYSS
                     EVLRGSYE"
     gene            complement(10638..11126)
                     /locus_tag="VFS75_03045"
     CDS             complement(10638..11126)
                     /locus_tag="VFS75_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677669.1"
                     /translation="MRFFKARTLRGLTSLEVVVGASIAGIIVAYAADAIGTFVSSARE
                     VADETQALYLAEEGLELARFIRDNDWNALDALSLSDPHYFEISAAAITTTTTPETVEG
                     FSRNFTLENVYRDGSTDDVVASTTGGAVEDSGSKYVTVTVSWGSPAKSVALMTILGNI
                     DQ"
     gene            complement(11128..11589)
                     /locus_tag="VFS75_03050"
     CDS             complement(11128..11589)
                     /locus_tag="VFS75_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4677670.1"
                     /translation="MRTLRTNGFTLAELIVVAAIVSLLAVVSLAVLSNLSSASALRAG
                     SGEVYRALLSARASTLASNGDTVYGVHLTTTSVTRFTGTAYVPGAASNQVYTFERDVN
                     ATGTLAISGADIVFERLTGTPSASGTVYVRSGSGNGTSTIIIEGSGLIELQ"
     gene            complement(11593..>12265)
                     /locus_tag="VFS75_03055"
     CDS             complement(11593..>12265)
                     /locus_tag="VFS75_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU4677671.1"
                     /translation="IIGILMMIYVVPTLTGTFVKLKITLPLSTRVIIGTSDFLTHHGL
                     LAIAGAILVIGAMMAAARTALGKRVLHLTFIKLPVIGNLVKQTNSAYTARTLSSLLAS
                     GVDVIGAIKITEDVLQNVHYKKVLAEAAGRVEKGSPLSETFVAHEEFYPVLVGEMILV
                     GEETGQISEMLTEVANFYENEVEEKTKDLSTIIEPLLMVVIGAGVGFFALAMIAPIYS
                     ISDSI"
BASE COUNT         2391 a         3736 c         3835 g         2303 t
ORIGIN      
        1 gcgaagaacg gtgcgttcaa gacactgaag gcggccggca tcatctaacg gagacggcca
       61 ctccatcgga agacgagaag atactgccga catcggcggt attttttcgc gtgatgccgc
      121 gcgcatcgag aaggtcgacg acctccttat gcgggtgccc atacgagttg ttcttgcccg
      181 cggagatgat cgcgtacgtc ggatggaccg cgtcgaggaa tatgggcgag gaggacgtcc
      241 gcgagccatg gtggccgacc ttgagaacgt cgcttcggag cgtcgacgtt ccgagggaga
      301 cgagatactc ctctatctcg cggggcgagt cgcccgtcat cacgtacccc gtcgttccat
      361 agcggagttc ggtcacgatc gatgacgcgt tgctctcgag tccggatggg tcgcggtcgg
      421 ggaagaggac cgagagcgtc gtcgtccccg ccggacccgc gccgaggtcg aagacgtcgc
      481 cgcgcctcgc gtagacgacg gtcgccccct catcacggac cgcgcggagg aactcctcga
      541 actcgggcgt cgacttcacg ttctcggtca ggacgatcgt gcggacgcgg taccggtcga
      601 gtacgccggc gagccctccg atgtggtcga ggtccggatg cgtcgcgacg accatgtcga
      661 tatcgcggtc gaagaaaccc atgaccgacg agaggtcgcg aaggaccgaa ccatcacggc
      721 cgccgtcgat gaggacctgc gtaccggacg gcgattcgat gaaggtcgcg tcgccttgcc
      781 cgacgtcgag gaaatcgaca tggaggagcg gcacgccgcg ctcctcgtca aaggacgcgc
      841 tcgcgacgaa cgaaagacac gcgagggaga tgagcgccgc gaagaacgga atgcggagcg
      901 atgccatatg ccttcattgt acttcaccgg cgactcacga gatgatgcta taatgaagcc
      961 tcaacgagaa gggttaccat tcttaacgac acgactatgc acacctacac cgcgaagacg
     1021 ttcgacctac cggccctcga cggcctctcc gagaagcaga taaaggtaca cctcggcctc
     1081 tacgaagggt acgtgaagca tgtgaatctc ctccgcgagc agatcgccga gctcaccgcg
     1141 ctcgataagg agaagtacgc gtacgcgata gcggagacgc gccgccgcct cggcttcgag
     1201 ttcgacggca tgcgcatgca cgaatactac ttcgaacaat tcgagggaga gaagggcagt
     1261 gatcccgaat cggcgctcgc gaaggcggcg gccgagaagt acggaagcgg cgagaacttc
     1321 atcgcgcaca tcaaggaggt cgccggaacg cgcggcatcg gctgggtggt cgtctacgcg
     1381 gacccgacgg gcggaacgct ccacacggct tgggtcgggg atcacgagct cggccagctg
     1441 tccggacttc ccatcatcct cgcgatggac atgtgggagc acgcgttcat ggtcgactac
     1501 gttcccgcgg agaagaagaa ctacatcgag gcgtttctca agaacgtaaa ctggcgcgtc
     1561 gtcgaagacc gctacgcgaa gctcggttaa tatcacagtc atgaaacctt cggacccgag
     1621 gacaacccgc accatcctct gggtcctttt cgtactcctc atcgtgctcg gcgcgtcgca
     1681 tccgcaggga ctcacgtttc cgctcctcat cggcgcattc ctcgttttct atatcgtcat
     1741 cggtaacttc gtgtacgacc cgctcgtcgc tattttcccg aaaggatgat caccgaaacg
     1801 gaaacgtcga cgccgcatct tcgttcgccg cgcgcgcgag ttcatgctcc tcgacgatgg
     1861 tccacccagc gtactcattc tcgaccgagc ggccgacctt tgggcgcctc gaggcgaata
     1921 gtcctaacgc ggtgacgccg tagagcgcaa gcgtgacagt aaacgggaac gcgggcagcg
     1981 acacggagga gaacgggagc gatgcgaacg cgcggacgac gatgatgacg tacgcgagcg
     2041 tgaggtgcgc gaagaagccg acaagcgtac cgagcggcgt cgcgatgagt cccgcgatgc
     2101 cggttacgaa cgtgaggagc atcgcgaggg ggacgacggg gaggacgaga acattgacga
     2161 gaactgaaac gagagagagg atgccggtct catagaggag gagcgggagc acgaaggcct
     2221 gcgtcgcgat ggtcgccgtc gcgtagccgc gaagtccgaa gcgctcgggc aggaatcgca
     2281 aacgcgcttc gaccggggta gagagcgcga tgagtccgag agtcgcgagg aacgataatt
     2341 gaaagccggg gtcgtgcaca aggagatacg gttcgaagat gagcatcagg atggcggcga
     2401 taacgagcgc gcggagcgcc gcatacgtac gcccggtcgc gtgcgcgaga ataaggagcg
     2461 atgccatgat ggacgcacgg acgacggtcg ccgacggacc gacgaggagc gcgaaacacg
     2521 tgatcgcggc gatccccacg atcgtccgcg tcctcgggaa gaggaagaac gtgagaacgt
     2581 gcatgagcgc cgcgacgacc accatgatgt tgtacccgga gaggacgacg atgtgcacga
     2641 cgcccgtctc gcgaaacatc cgctcgaggt catcgccgag cgcgcgacgg acaccgagga
     2701 ggagaccctc ggcgagtccg gactccggca tagggagcgc cgcctcgacc gccgacatga
     2761 accgatgttt ccccgcgtag agacgagcga gcggcgaaga cgtgtcccgc gcgagcaccg
     2821 ctaccgtcgc gaacgggacg gtcacctcga tgccgcgagc cttgaggtat cccgggtaat
     2881 cgaacgtcct gccgccattc gtcgaaaacg cctcgggagc gcgcatcgtg ccctcgacga
     2941 cgaccgcgtc accgtactcc ggaaccggga tgcctccctc ccgcgaaacg ctcgcgagca
     3001 cgagctcatc catgccgctc ggccgcacga cgaactgcgt cgttcgctca ccgacctccg
     3061 gttcgcgaac gacgcgtccc gtcacggtca cttcttcgcc gatgtatgac gagagctcgg
     3121 acggagcgcg ttctgcgtaa tcgagtcgaa gtgcgccgag cgcgagcgcg aaacacagaa
     3181 gcgcggcgac gcggagcgtg cggctccttt cgatcgacga cgcgaaaaga agggcgagcg
     3241 cggtgagggc gagcgtgagg aggagggcgc ggtcgaacgc aaaaaaagag cgggccgcta
     3301 tcccgctcgt aaacgcaagt gcgactccga agaaaaggag cccgtttggg tgcgtaccgc
     3361 tcgttactct cgtttccatg tcgcgacgag ttcgtccgcc tcttccttcg agaaggcttt
     3421 cttgaacttc gcgtgctttc ccttcacgcg acgctcgcac tcggcccacg tcttatgcgt
     3481 cataacgacg ccgtctaccc gtgaaacgta cgcgtacgcc gttcctttcc gcgaggaaga
     3541 ggacgagacc gcgcgcgcct tcggcattcc cttgaggaac tcacggtact ctttggcgct
     3601 tcctctgaag agccgtacgc gctcgccacg agcgaagccg ttcgcgatca cgtcgacccg
     3661 ctcgttcccc gcatggcctg cgtggccgcg aacgtgttcc caggtgacgt cgtgttcgtc
     3721 gttcgctcgt ttcaattttt cccagaggtc gcgattcagt acgtctcgct tctccttcgt
     3781 ctgccacccg tttcgctccc accccttcac ccacttcgtg atgccgttta tgacgtagct
     3841 cgaatcagtg cggatggtca ccgtggcacg cgcggggatg tgggagagcg cctttagggc
     3901 agccgtaagt tccatacgat tgttcgtcgt gtcctttgcg aagccgccca gttcgacgac
     3961 cgattgctct ttcccctctc gcgtctcgat ggacgccccc caaccgcccg ggccgggatt
     4021 cccgatcgag ctaccgtcgc agaagatggt gacctctttc atctggccag tatagcaaag
     4081 agtctgagac gatatcgacg agcgtcaggt cactaattcg cttcgcttct cacctcgcgg
     4141 tttcgcctcc ttttaggtcg gcgaaacatc gctccggctc ctcgtcgact ttgagatgaa
     4201 gcagttcatt tcaaacgatg tcgacgaggc gtaacctcgt ctcaagacga cccatgaagt
     4261 acgagcgctc gacgtgcgcg atgaggtcga aggtctcccc ttccttcgga accttcgtga
     4321 aggagtccgg gagcgcgaag aacgaaatgg cgtcgatgcg gccatgagcg gtctctaagc
     4381 ggagcttcgt gtggttcttc gtctttccga agacttcgac cgcacgcggc atgacggacc
     4441 gtattttgaa aagcggcttc ggattaccga cgccgtaggg cgcgagcgcg tcgagggcgc
     4501 ggaggagccg gtcatcgatg tcgtcgagcg agagctccgc atcgaccgcg ttcgccacat
     4561 ctcccacgag aacgtccgag ccatgttccg cgtacgcggc atggagcgcc tcggggagcg
     4621 tgtggatgcg ctcatcgctt accgagaaac cgcctgatgc gtggtgaccg ccgaacgtga
     4681 tgaaggcgtc cttcgcgagc tccatgagcc gcacgatgga gacgccgccc gcgcttcggc
     4741 acgagccttt gatgataccc ttcccgtctc tcccccagag aaacgccggc cgaccgaact
     4801 cctccgaaag cgagttcgcg acgaggccga ccagggatgg acgccactcc ggattcccca
     4861 tgacgatgac ctgcggcagt tcgatgagct cctcgatgcg tcgcttcgcc tccttcacca
     4921 tgctcccgac gacgcccttt cgttcgttat tgagtttctc gagatgatac acgcgtgcgc
     4981 cggcgtccgc ctcgtcgcgc gtgcggagca tgtgaaacgc gtcttcgggg tcgtccatgc
     5041 gcgacgcggc gttgatgcga ggcgcgatgg tgaacccgat gtcgtcctcg gtgaggaagc
     5101 gctgattcgc acctgccttc cgaaggagtt gttggagacc gggccgacgc gactttcgaa
     5161 ggaccgagag tccgtagcgc gcgaggacac gactctcgtc ttgaagcggg accatatccg
     5221 cgacggtcgc gaggccgacg agatcgagcc accacttctc cataccttcc ttaagcgtga
     5281 tggtcccgtc tttccgtcct atggagagga cggcaccgac gagcttccaa gcgaccgcgg
     5341 tgccgcagat accggagaac gggtacgcgt cgtcgcgctt ggggttcacg accgcgtacg
     5401 cgggcggaag agtctccgca ggctcgtggt ggtcggtcac gatgacgtcg atgccgaggt
     5461 cgttcgcgcg ctcgaccgct tcgtggtcgg tgatgccgca gtcgacggtg atgatgaggg
     5521 acgcaccgcg ttccttgaac gcgggtacgg catcgggatt gaacccgtac ccctcgtcat
     5581 gtcggtgcgg aatgtagttc tcgaaattgt cgtacccgat ctcggtgagg aagtcgtgga
     5641 ggagaacgcc gccggggata ccgtcgcagt catagtcggt atagatgacg atgtgctcgt
     5701 tctcgtgaac ggcgcgaaga acgcgcgtcg cagccttctc catgtcggtg aggagaaaag
     5761 gatcgtgaag gtcgctctcg aagtcaggct cgaggaaacg gcggatcgac tcggcatcgt
     5821 tgagaccgcg attgaagagg agctggcgct ggatgggatg atacccatta agcgcatcat
     5881 cgagagtgtc gggggccttg gggagcagtt cgtacgtacg tcgcggggcg ttcatcgtgc
     5941 tccgatttta ccacgggggg cgcctatggc agaattacgg catgaacgac atattcgaca
     6001 agatactcag gaaggagatt cccgcccaat tcgtgtacga ggacgatgtc tgcgtcgtcg
     6061 tcatggacaa atacccggtc gttccgggtc aggtcctcgt catcccacgt gactgcatcg
     6121 actatttctt cgagatggat gatgtgaagt accaccattg tctctcgatc gcgaagcgca
     6181 tggcggcggc gctcgacgtc acctacgaga ccgagcggac ctgtatgatc atcgagggat
     6241 tcgaggttcc gcacgcgcac ttgaagctct atccggtcac gcgccgcgag ttcacgccgc
     6301 acggcggcaa ggaggtcgac gatgcgacgc tcgagaagga ggcggagaag atcaaagcga
     6361 acctccgatg aaaaacggcg cccaaggcgc cgtttttctt atgggagcat tgcgaccgcc
     6421 gtccattcca tcgattcgat ggtccggccg tcgccactaa agcgaacgac gccgcggagc
     6481 ctctcgcccc gaagcgctcg gggaagccag tgttcgtcgt ccgcccacat cttgtcgagg
     6541 ggtatctccg agaacgaaaa ccatgccggt cgcatctcct ccgtctccct aggttctccg
     6601 tcccagcgcc gtacgaaaaa tgagaacacc tcgaggtgtt tcccgtcttt gaagtgaaat
     6661 tcgatgacgg acgccggctc gaggtcgtct ggagagacgc cgacggtcga ctcttccttg
     6721 agctcgcgaa ccgtcgcttc cgcgatcgat tcgccatcct cgaccttgcc gccgaagccg
     6781 ttccagtttc cctcgccgaa gccgcgtttc ttgagggcaa ggcatatacg gtccgcgtcg
     6841 atgaggtacg cgagcgtgag ttgctttttc atagctatca agactccttg ggaagcgctt
     6901 cgatgccggg aagcgtccgt ttctcgagga actcgagcat cgccccaccg gcggtcgaga
     6961 ggaaaccgaa cttatcttga agtcccatcg attctatcgc cgcgaccgtg tcgccgcctc
     7021 ccacgaccga gtatgcgctc gaggatgcga tgagatcggc acaccgcttc gtcgccgcgt
     7081 cgaaaccgcc ttcgtaatag ccaagtggtc cgttccagag aatggtctcg gcctcggcga
     7141 tgtacggctc gatcatctca agtgatgcga ggccgacatc gactatcttt tcgtccttcg
     7201 cgacctcgtc gaccgctgcg gtcctcgtcg tgccgccctt ttcgacgaca acatcgatcg
     7261 ggagcttcag gttctcgcga gtgagcagtt cgtttcccgt gaggtcgacc ggcgacacca
     7321 ccgatgtgcc gacctcgtat cccttaccct ttaaaaagtc gttcgcgagc gcgccgccga
     7381 tgaacgtgcg cgtgtacgtc ttcgagtact cctcgataag gggctccttc gtctcgtact
     7441 tcgcaccgcc gagaacgaag agcgagggac tcgcgggtgc gacgaccttc gtgagttcgt
     7501 tcaattcctc catgaagact ataccggcga agctcggaag gaacttcggt atgccgacga
     7561 tcgatgcgtg ctcgcggtgc gagaccgcga atgcgtcgtt cacgtagaag tcgccgtacg
     7621 aggcaagctc tcgcgcgaac tccggatcgt tcgccgtctc gcgtgggtcg cgtctaaggt
     7681 tttcgaggag gagcgcggta ccttccttca tgtgcgcgac cgcgccgtaa acctcgtcgc
     7741 cggtcacagc cgggacgtac cggacgggaa tatgcttcgc gaggagggac acgagcgatt
     7801 cggtactgtt ctccggatct cgtccgacgt gcgtgagaat gatgacgcgc gccccgcggt
     7861 cgagaaggtg gacgaaggtc ggaatggcgc ggcggacgcg aaagtggtcg gcggcgatcc
     7921 caccttcgag cggaacgtcg agcgatgcgc gaacgaggaa ccgcatcccg aagatgtcgg
     7981 taagggcttc gacggaacga ggcttcgtgc tcatgtacgt gttgcgttta tgatatcgat
     8041 aaattcaccc gctttgaggc tcgcgccgcc gacgaggaat ccgcgaacgg tcccctcgac
     8101 gaaaagctct cgcgcgttct tcgcgttcac cgaaccgcca tagaggatgc ggacgcgcgt
     8161 tgcgtacgtt cgtccataga tatcggccag aaccttctcg atgaagaggc gcatctcgtg
     8221 aacgtccgcg gccgtcgcgt tctcgccggt gccgatcgcc cacacaggtt catatgcgat
     8281 gacgaccgct ccgagcttcg ccttcccgag cgacgacagc gcagctcgta tctgtccctc
     8341 gacggtcgtg agatagtgcc cggcgtggtc gcgcttcgtc tcaccgacgc agaggacggc
     8401 ggtgagcccg ctcttcacga ccgacagaag cttccgattc acctctgtgt ccgtctcgcc
     8461 cgcggcacgg cgctccgagt gaccgacgat gacgtgtgtc acgtcaaagc tctcgagcat
     8521 cgggatcgat acctcgccgg tgtgggcacc ctgcttttcg tcgtgcacgt tctgggcgcc
     8581 gagcgcgaac gacttgctct cgcttcgcac ggcacgaagg gtctcgaggt acggatacgg
     8641 cggcgcgatg acgacgtccg tgcccgtcat gcgggcgagg cgcttcttga ggtcgctcgc
     8701 gagtttcttc gcgagcgaaa ccgacgtcgg gttcatcttc cagttaccga tgacgagggg
     8761 tgacgttttc atgatagtga aatagtacca cggccccttc ggtataccgg aggggccgtg
     8821 aaaaaacggt gaagctactc gctctcctgc caggaggtga tcgaataccc cccacccggg
     8881 cccgacgtga agagaaggtt cgcgaggccg gattcgtacg tgacctccgc cgagttcttg
     8941 aggtggatat ggtacgcggt gagctcgttc acggagacgt tgttctcgag gagcgcctca
     9001 ccatgccccg cgtagagaag gaggtcaccg ttcgcgctgt tcgagacctc gatcgcagtc
     9061 tcgccgccgc cgtcctcgta cgatcggttc tgagagagaa ggagcacgta tgagcctgca
     9121 ccgcttccaa cgaacgtcgg gctatttccg agatcgatct tgctccccgt cgtccggttc
     9181 gcggggtcat ctgcgatgac gggcacggac attccagaga gagaggcgtc gacgcgaagc
     9241 gtcgcgttcg tgtcgtcgat gttccccacg acccacacgg gacccgcgag agtgacgtcc
     9301 gtaccgctct tatcgaaatg aacattgcag ttgatcttca cgggtccgat cgtcacatct
     9361 gactgtatga ggtacgtacc gctcgaacag cgcgggtccg tagccgcgat gacgccgcct
     9421 gcctcggccg ccgcctccca ctccgcgacc ttatcatcgt cgatgggcat cgtcgccgtc
     9481 gcttggtccg cgctccccgg gtgcgtcgaa ccgaagacga cggtgccggt catcgtttgg
     9541 tagtacgcat cgagtccgat gatggagctc gtaatggtat gcgcccacgc gttcccgacg
     9601 acgtgaaccc cgttcgcgcg gccggtcggg cccgccgaga tggcgtcgcc ccagacgaag
     9661 ttgaagttcg tgccgacgat ggagccgttc gagaatatgt ttccgaagac gtgcgacgtg
     9721 ttgtccattt taataccgcc gttgccgctc tggaggccgt agttgaacga cgcaccatcg
     9781 ccatagaaga gctgtgcgga cgaccgccgt atacgggagt cgtcatcgcc cacggcgagg
     9841 aaatcataga gcttaccgtt tatggtacgc gtcacggcga cgtccgcggt ccccacagtg
     9901 aaggattcgg tcgccgagta gttcatgccc gctcggtaac ggtagaccgc atcctcgatg
     9961 gcggcgtcgg ccgcgtagta actctccttc ccgagtttca tatctttaaa ccgcgccagg
    10021 tcgtcataga cgcctccgcc gatcgagagg acgagcgtga gcgatatcgc gaggaagaag
    10081 acgacgaaga gaaggagcgc cgccccgcga gtattctgga tgccgacgcg ttcgttattc
    10141 ataggaaccg cggagaactt cgctcgaata aaaagtacgc gtgctcgtgg aaacactccc
    10201 cacgcgcgtg agcgtgagcg cgacccgcac gagttccgtc gccgtgccga catagtgtgt
    10261 gaacctgaga tcatcgaccg tgacatcgct tccagtgagc ggccccacct cctccccgtt
    10321 ctcggaaaga atgacgcgac cgccggagag cgagaacgtg gtggtcgttg cccccgcgat
    10381 gagcgtgagg acgccggggg acgtcccgag cgtactcgct cccgtatcga cgatcgtcgc
    10441 gccacggagc gcgcttccca tcctctcgag cgcgacgctc gcggattcgg tgagcgcgcg
    10501 atcatcgcgg ttcttgagaa gcacggtgtc gagcgaatga aacgtcgtga tgagcccgac
    10561 cgcaatgagg agcatcacgg aaacgtacac gagcatctcg atgagcgtga agcctctttt
    10621 gttttgggat acgtgcgtca ttggtcgata ttaccgagaa tcgtcataag cgcgacggac
    10681 ttcgctggac tcccccagct caccgtcacg gtcacgtact tcgagccgct atcctcgacg
    10741 gcgccccccg tcgtcgacgc gacgacgtcg tcggtcgaac catctcggta cacgttctcg
    10801 agcgtgaagt tccgcgagaa tccttcgacc gtctcgggcg tagtcgtcgt cgtgatagcg
    10861 gcggcgctga tctcgaagta atgcgggtct gaaagggaga gcgcatcaag cgcgttccag
    10921 tcattatcac gaatgaaccg cgcgagttcg agcccctcct cggcgaggta cagagcctgc
    10981 gtttcatcgg cgacctcgcg cgcgctcgag acgaacgtgc cgatggcgtc ggccgcgtac
    11041 gcgacgatga tgccggcgat cgacgcgccg acgaccacct cgagcgaggt gagtccgcgt
    11101 agcgttcgtg ctttgaagaa tcgcatgtta ctgaagttcg atgaggccag acccctctat
    11161 gatgatcgtc gacgtgccgt tcccgctccc gcttcgcacg tacaccgtcc ccgatgcgct
    11221 cggcgtcccc gtgaggcgct cgaagacgat gtcggcgccg gagatcgcga gagtccccgt
    11281 cgcgttcacg tcgcgctcga acgtgtacac ctggttcgac gcggcgccgg gcacgtacgc
    11341 cgtaccggtg aatcgcgtca ccgaggtcgt cgtgaggtgg acgccataga cggtatctcc
    11401 gttcgaggcg agcgtgctcg cgcgcgcaga gaggagcgcg cggtacacct cgcccgaccc
    11461 ggcgcgaagc gcgctcgcgc tcgagagatt actcaagacg gcgagcgaca cgacggcgag
    11521 aaggctcacg atcgccgcga cgacgataag ctcggcgagc gtgaatccgt ttgtacggag
    11581 cgtacgcatg tcctagatgc tatccgagat cgagtagatc ggtgcgatca tcgcgagcgc
    11641 gaagaagccg acgcccgcac cgatgacgac catgaggagg ggctcgataa tggtcgagag
    11701 gtccttcgtc ttctcctcga cctcgttctc gtagaagttc gcgacctcgg tgagcatctc
    11761 cgatatctgg ccggtctcct cgccgacgag gatcatctct ccgacgagaa cggggtagaa
    11821 ctcctcgtgc gcgacgaacg tttccgagag cggacttccc ttttcgaccc gccccgccgc
    11881 ctccgcgaga actttcttat agtgaacgtt ctggagaacg tcctcggtga tctttatcgc
    11941 gccgatgacg tcgacgccgg aagcgagaag cgacgagagc gttcgcgcgg tatacgccga
    12001 gttcgtctgc ttcacgagat tcccgatgac gggaagtttg atgaacgtga gatggaggac
    12061 gcgtttcccg agcgccgtcc gcgcggcggc catcatcgca ccgatcacga ggatcgcgcc
    12121 cgcgatcgcg aggagtccat ggtgcgttag gaagtcgctc gtaccgatga tgacgcgcgt
    12181 cgagagcggg agggtgatct tgagtttcac gaacgtaccg gtcaacgtcg gcacgacgta
    12241 gatcatcatg aggatgccga tgatg
//
LOCUS       DASTSP010000068        10150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_361264, whole genome shotgun sequence.
ACCESSION   DASTSP010000068 DASTSP010000000
VERSION     DASTSP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361264"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..367)
                     /locus_tag="VFS75_03060"
     tRNA            complement(297..367)
                     /locus_tag="VFS75_03060"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Pro,seq:tgg)
     gene            complement(462..860)
                     /locus_tag="VFS75_03065"
     CDS             complement(462..860)
                     /locus_tag="VFS75_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU4677672.1"
                     /translation="MARHNEIGKMGESLAADWLRAHGFSIIEQNHRQKWGEIDIVARE
                     TAGGVHFVEVKTVSYETREVLEHAVSHGTWRPEEQVHTAKRARLKRVIESWLAAHPDC
                     DDWQIDVVAVRLVPREKFGTIKLLGNVVFE"
     gene            1064..1861
                     /locus_tag="VFS75_03070"
     CDS             1064..1861
                     /locus_tag="VFS75_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677673.1"
                     /translation="MQMLSFMSVIRRALLEAVVLTCDNVGMEGPSGIATPAVLGLGAA
                     VAGILGFFYLNVASPVASMLIDPADRVVTRGEIFDVNVVIRSSISVNVFAGELYFDPS
                     ILAVDSIDYNTSIADLWAERPWYSNGNGTLNFAGGSTKGGFEGEGTLIKVTFRAIGEG
                     AGVISIHDPRILKHDGLGTDAEIDRPIDAIFTVRAENGDVLTPQSNTMRFHVVKEAPS
                     TDLNGDGRHTIADISVFLLHLSTDDERYDFNMDGRVNLEDLKIILGS"
     gene            complement(2050..2120)
                     /locus_tag="VFS75_03075"
     tRNA            complement(2050..2120)
                     /locus_tag="VFS75_03075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2086..2088),aa:Gly,seq:gcc)
     gene            2251..2517
                     /locus_tag="VFS75_03080"
     CDS             2251..2517
                     /locus_tag="VFS75_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677674.1"
                     /translation="MFESPENKKEYVNLRSAEDLADRLDVELQDNGSLSSDSLESLAM
                     KIQDLIEVQRSLSGAAEQEQVNAMIAKLKNIEIRNREGGLKQAA"
     gene            complement(3242..3313)
                     /locus_tag="VFS75_03085"
     tRNA            complement(3242..3313)
                     /locus_tag="VFS75_03085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3278..3280),aa:Arg,seq:tcg)
     gene            3382..4854
                     /locus_tag="VFS75_03090"
     CDS             3382..4854
                     /locus_tag="VFS75_03090"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4677675.1"
                     /translation="MIIRSEIPKEVQHVVDTLEGRGFEAYLVGGCVRDLILGKKPKDW
                     DVTTDAHPGEIESLFPETYVNNDFGTVGVVNKEASEPSLTIVEVTPYRTESTYSDARR
                     PDSVAFGVSLEDDLKRRDFTVNAIAYRVKGEKLVDLFGGEADLAAKRLTAVGDPRERF
                     AEDALRMMRAVRFAVELDFVIEADTMAAIAENKDNLGRISKERIRDEFSRILASKHPM
                     QGIVLLEKLGLLPYVAPDLLRGIGVMQNQAHSYDVYEHNLRTMQHAADKEWALDLRLA
                     GLFHDISKPETRRWSKEKNDWTFHGHEVVGAKVARRALQELKFPRERIDRVVKLVRWH
                     MFFSDPDTVTLSAVRRTIANVGPENIWDLLKLRRCDRIGTGRPKEQPFRLRKYTAMVE
                     EALRDPISVKMLKIDGSRIMAITGEKPGPKLGHTLHALLEEVLDEPGKNTPEYLEGRA
                     QILMKMPVEELAALGEKGREKLAGEEAAAVADLHRKHKVD"
     gene            complement(5357..6148)
                     /locus_tag="VFS75_03095"
     CDS             complement(5357..6148)
                     /locus_tag="VFS75_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU4677676.1"
                     /translation="MTIYSWNVNGIRAVMKKGDFGRFMETFAPDVLCLQETKAKQEEA
                     AVDLPEYDEYWCHSEKKGYSGTAIFTKVAPSNVLLGLPEDIARKYDLKDDYGDTAREG
                     RITALEFENMYVVSVYTPNAKDDLSRIPVRVAWDAAFLEYMKRLDSEKPVVFAGDLNV
                     AHTPIDLARPKENEGHKGFTKEERIGIDAMLAAGFVDTFRLFEKEGGHYTWWSHWGAA
                     RERNVGWRIDYVMVSEALTPLVKKASIHPMVFGSDHCPVSIELAL"
     gene            complement(6236..6757)
                     /locus_tag="VFS75_03100"
     CDS             complement(6236..6757)
                     /locus_tag="VFS75_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677677.1"
                     /translation="MQGDKPSHSQKPCVVFFATQSGATHESFNRERCYVDVIVPAREL
                     LESGLVRGVVFVIECEKDAPGSEVPEMIYDKDQVRMTPSAKFILENLRKHGHISEVPN
                     YCVDVLDIDVWKNLPESRFGHIANFAKEQGHAVLYFAENQPFPSLGAVMREIVSLREG
                     EVRVIDPPIRAAA"
     gene            complement(6927..7358)
                     /locus_tag="VFS75_03105"
     CDS             complement(6927..7358)
                     /locus_tag="VFS75_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677678.1"
                     /translation="MTEHSRRPALAIILPQADSPFSAARCDRELVEPVLEILRQSVVD
                     QCIIVLQREDVAPGPTETHLKNLRVVQQALYREDISLVQPGDSLAESMESCRIRAETC
                     GADALLVIDRGAIPHHSVLLHERRTLKKSEVRLVSVQNAVA"
     gene            7672..7983
                     /locus_tag="VFS75_03110"
     CDS             7672..7983
                     /locus_tag="VFS75_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677679.1"
                     /translation="MDHFLEKHWAYYVKDNPEGYWFKRKLYGWGWTPVTKEGWTVMAG
                     WLILVILNSFRLEKTASADFANWFVLETLALSLILLVICYKKGEKPRWQWGLSKDQLR
                     D"
     gene            8069..8887
                     /locus_tag="VFS75_03115"
     CDS             8069..8887
                     /locus_tag="VFS75_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU4677680.1"
                     /translation="MDNAQEGGVPENTTNETPSVPVTGTEAAATTADEASIPAAPVTE
                     PARRRFFEGKGAMVGAIVAALILIAGAGYYVYSQYYAKGGIVAVVNGRNIYRSELDES
                     IAMVGENAQAYGIDETSSTANKDKQDRAMEVLVDNALLITAAENAGFDADDETVQKSY
                     DDLVAQIGGEDALKSQMAAVGLTESRLRDNIRERVLVGKYIESVNPSDTISVSDKEVD
                     DFIAQLKANNVELPDDMTTLRPQIEAEIKAQKQQQLVDALITKLKGEAQIDMRI"
     gene            8980..9216
                     /locus_tag="VFS75_03120"
     CDS             8980..9216
                     /locus_tag="VFS75_03120"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEU4677681.1"
                     /translation="MVDKLNRYTKDRPRLKKVVGWAFVVVGFVALALPIVPGAPLVFV
                     GFEVLGIRFIFTERLRGLIGLDRAVPEEVPERSL"
     gene            9261..9845
                     /locus_tag="VFS75_03125"
     CDS             9261..9845
                     /locus_tag="VFS75_03125"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEU4677682.1"
                     /translation="MRTSLIVLGAVIVLLALGGIAMWIPKEWGNPAATPPAPATTTNV
                     GGTVACTMEAKICPDGSSVGRVPPSCEFAPCPTAATSSTDVVIIDAPRPNATVTSPVT
                     VTGKARGNWYFEGSFPIVVVNWDGLIIGQGVAHADGEWMTTDYVPFSASISYALASET
                     PYARGSIILKKDNPSGLPENDDSREIPVTFSEVR"
     gene            9842..>10150
                     /locus_tag="VFS75_03130"
     CDS             9842..>10150
                     /locus_tag="VFS75_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677683.1"
                     /translation="MIESYFLVFLKIHAATILLLMPTILYADSIGLAWMRGVTPVVSK
                     RELAFVHRVVFVGLTLMVLSGALMAYPYLGFLLSERSFLLKMPFVLALIVNGIFIERS
                     L"
BASE COUNT         2220 a         2921 c         2893 g         2116 t
ORIGIN      
        1 tcaaaaatcg ctcctccggg gacgtacccc gagaagcccc tcctggtttc ccgtttggct
       61 ttttgcggtt ctccgccatg tggaaatcat tataagcgat gacggccaaa aaacccactt
      121 ctttcagtct aaaaatatga acgtctcgtg aattccaaaa cctcaaataa agtatagtta
      181 gggccagttt tgaagtgcca cacgtgcgat caaaaaagat tcctgcgcta aaccggtgca
      241 cctttcgcag aaactgctca agatcttcga ttcccgtgcc aaaaacgggg tttttgtcgg
      301 ggtgccggga atcgaacccg ggcctcgtgg tcccaaacca cgaacactac cattatgcta
      361 caccccgaaa gtcgaatccg acgatgatac caccctgcca ctgaaaaagc gagaaaaaac
      421 ctctaacttc tgtcgtttca cgtgattcat ttcacgtgaa actactcgaa gaccacgttt
      481 ccgagcaatt tgatcgtccc aaacttttca cgtggaacga gtcgtacggc gaccacgtcg
      541 atctgccaat catcgcagtc cgggtgcgcc gcaagccatg attcgataac gcgcttcaat
      601 ctcgcgcgct tagccgtatg cacttgctct tcgggccgcc aagttccatg tgaaaccgcg
      661 tgttcgagga cttcccttgt ttcatatgaa acggtcttga cctcaacgaa atgaactcct
      721 ccagccgttt cacgtgcaac aatgtcgatt tctccccatt tttgccggtg attctgctca
      781 ataatcgaga acccgtgtgc acggagccag tctgccgcaa gagactcgcc cattttgcct
      841 atttcgttgt gccgagccat gtagtttcac atgaaactat tacaaattcg ctggtatggc
      901 gctacgtttc acgtgaaaca tctgtcattg tgcctcgtat ggctcactga caatgaatat
      961 tgtgaaatta agccgttttt taaggacaaa ataagtcgtt ttttatgtcc tttatacaca
     1021 tatcaacggg gttgtcccca gtttccaaca tttcggttct tgcatgcaga tgctgtcctt
     1081 tatgtccgtt atccgacgcg cgctattgga ggccgtcgtt cttacgtgtg ataatgtcgg
     1141 tatggaaggc ccttctggaa tcgcgactcc cgctgttctc ggtcttggcg cagccgttgc
     1201 cgggattctc ggttttttct acctaaacgt cgcttcgccg gtcgcgagca tgcttatcga
     1261 tccagctgac cgcgtcgtta cgcgggggga gatcttcgat gtcaacgttg tcatccggtc
     1321 gtcgatttcg gtgaacgtct tcgcggggga attgtatttc gacccctcca tactcgccgt
     1381 cgattccatc gactacaaca cctccatcgc cgatctttgg gcggaacggc cctggtacag
     1441 caacggtaat ggaacgctga atttcgcggg tgggtccaca aaaggcgggt tcgaggggga
     1501 aggaactctc attaaggtta cgtttcgtgc catcggggag ggggcggggg ttatctccat
     1561 tcatgacccg cgtatcctca agcacgacgg gctcggaacg gatgcggaga tcgaccgacc
     1621 cattgatgcg atattcacgg tgcgtgccga aaacggcgac gtgctcaccc cgcaatcgaa
     1681 caccatgcga tttcatgtcg tgaaagaggc cccatcgacc gacctgaacg gggatgggcg
     1741 ccataccata gccgatataa gcgtcttcct gcttcacctc tctacggacg atgagcgcta
     1801 cgacttcaac atggacggca gggtgaacct cgaagacctt aagataattc tcggctcgta
     1861 gcgttccacg cgaaccgttt cacgggaaac gaagaaagac ggtcgaccag aagggtgcgg
     1921 ccagggagaa tcgaaactta cagtttcagt acccgttgtc gctatttttt caaaatagca
     1981 ggcaacggtt tcgattctcc agcacagaca cgagaaaaac cgggacaagc ccggcttttc
     2041 tcgtatctgt gcggccaggg agaatcgaac tcccgactaa tccttggcaa ggactcgtta
     2101 taccacttaa ccatggccgc gcttcatgcc cgaaaatcga gctcgcgtga ttatagtatc
     2161 tccgccgtct ttctgcaatc gagaccccgg taccgacgtt tacagattcg cgccgggcgc
     2221 tatactgctt gtaccagttt aagtaaccat atgttcgaat cacctgaaaa caagaaggag
     2281 tacgtgaatc ttcgctcggc agaggacctc gccgaccgac tcgatgtcga acttcaggat
     2341 aacggctcac tctcgtcgga tagcctcgag tcgcttgcca tgaagataca ggatctcatt
     2401 gaggtacagc gctcgctaag cggcgcggcg gagcaggagc aggtgaacgc gatgatcgcg
     2461 aagttgaaga atatcgagat tcgaaacagg gaagggggtc tcaagcaggc tgcatagcgt
     2521 ttgagaataa aattagggag caggcgctcc ctaattgtcg aagtggagag agattggcct
     2581 cgtgaacgac cctcgaggat cgagttcttc tgccgtttcc tcggggtagc gctcgcgtac
     2641 gaggcatacc cgccccctgg cgcaggcacc ggcatttctg cctcttgtcc tcggaacgtc
     2701 gcaggggaga acatcctcgg tgaaacggcc ggcgtgtttg agcatgagcg gacaaatgat
     2761 tcggccgtcg tctgaccgta tcgtgtacgt cggtcgtacg aaccagatgc agagccgcag
     2821 gtagagcttg tactgccgtc gtatgcgatg cagtcgacac atgtatccct ccgtcaaaga
     2881 actatgaaaa aaccgcccgg aagggcggtt ttttaagaaa aaagcatccg cggcgagcca
     2941 gtggatgcgt tcttcgtctc atcattttga tggtatgggt ttcacgggga ggagtcaacg
     3001 aggcgtatgg gataaggagg cgggactcgg tccttacaga atcagtacac ctttcgcaga
     3061 aactgctcaa ggtcttcgat tccctctggg tgccaaaatg caacagccgc gacatttatg
     3121 cgggtcctcg gtccttacag gacctgtaca cctttcgcag aaaactgctc aaggtcttcg
     3181 agtccctctg ggtgccaaaa tgcaacagcc gcgacatttt catgtcgcgg ctggcatttt
     3241 gtgcacccag agggactcga acccccaacg actgatccga agtcagtaat gatatccatt
     3301 tcaccatggg tgcgtgagac gtcgggatat catacggcca ccgtacggaa attgcaatga
     3361 aattttgcta caatcaagtc catgataatc cggagcgaga taccgaagga ggtccaacac
     3421 gtcgtcgaca cgctcgaggg acgcgggttc gaggcgtacc tcgtcggcgg atgtgtccgt
     3481 gacctcatcc tcggaaagaa gccgaaggat tgggatgtca cgaccgacgc gcaccccggg
     3541 gagatcgaga gcctctttcc ggagacgtac gtcaacaacg acttcgggac cgtcggcgtc
     3601 gtcaacaagg aagcgagcga gccgtccctc accatcgtcg aggtgacgcc gtaccgtacc
     3661 gaatcgacgt attctgacgc acggcggccg gattcggtcg cgttcggcgt ctcgctcgag
     3721 gacgacctca aacgtcgtga tttcacggtg aacgcgatcg cataccgggt gaagggcgag
     3781 aagctcgtcg acctctttgg cggggaagcg gacctcgccg cgaagcggct aaccgccgtc
     3841 ggcgacccgc gcgagcgctt cgccgaagac gcgctccgca tgatgcgggc cgtccgcttc
     3901 gccgtcgagc tcgatttcgt catcgaagcg gacacgatgg ccgcgatagc ggagaataag
     3961 gacaacctcg ggcgtatctc gaaagaacgc atccgcgacg agttcagccg cattctcgca
     4021 tcgaagcacc cgatgcaggg catcgttctt cttgagaagc tcggccttct gccgtatgtc
     4081 gcgcccgacc tacttcgcgg catcggcgtc atgcaaaacc aggcgcatag ctacgacgtc
     4141 tacgagcaca acctccgaac gatgcaacac gcagccgaca aagagtgggc gctcgacctc
     4201 cgcctcgcgg gccttttcca cgatatctcg aagccggaga cgcgccgctg gtcgaaggag
     4261 aagaacgact ggaccttcca cggtcacgag gtcgtgggcg cgaaggtcgc acggcgtgcg
     4321 ctccaggagc ttaagttccc gcgcgagcgc atcgaccgcg tcgtgaagct ggtccgatgg
     4381 cacatgttct tcagcgaccc ggacacggtg acgctctccg cggttcggcg gacgatcgcg
     4441 aacgtcggcc ctgagaacat ctgggatctc ctcaagctcc gtcgctgcga ccgtatcgga
     4501 acgggacgac cgaaagagca gccgtttcga ctccgaaaat acacggcgat ggtcgaggag
     4561 gcgctccgcg accccatttc ggtgaagatg ctgaagatag acgggagccg catcatggcg
     4621 ataaccgggg aaaagccggg gccgaagctc ggccatacgc ttcacgcgct cctcgaggaa
     4681 gtgctcgatg agccggggaa aaacacgccc gagtacctcg agggaagagc gcagatactc
     4741 atgaaaatgc ccgtcgaaga gctcgccgcc ctcggcgaga agggaagaga gaagcttgcc
     4801 ggcgaggaag ctgccgccgt cgcggacctc cacaggaagc acaaggtgga ctgaccgaga
     4861 aacagccccc ggaacccgtt ctgccacagg cttttctctt attcgcccca ggatggacct
     4921 ctaggatgtc cgggaccttc ggtccccgac atttttgcac aataaaaaag cccgttggat
     4981 cgaatgccga ggtgtcggcg cccgatccaa cgggcttttg tgggggtcga gtcacgagtg
     5041 gaactttgtg ttccggtgga tggaacttgg agagttctgc atcctgtggg tgactcttgc
     5101 ttcttggtga ttcttgcgtg aatcagtggt gattctagcg ttcctcacct gtgatgtact
     5161 tagagttttt tggaggtatt aactcgattt tataatgtgc tctttaaggg gtaaccttcg
     5221 ttacctgctc gccctttctg ggcctacgaa gagtgtatgt cctttatgag gagctgtaaa
     5281 ggacatatgt ggggatatct gtggatagat gtcctttata aaggacatgg ccaaaaggaa
     5341 gcgagggtaa gccattttaa agagcgagct cgatggatac ggggcaatgg tcgctcccga
     5401 agaccatggg gtgaatgctc gccttcttga cgagcggggt gagcgcctcc gagaccatga
     5461 catagtctat ccgccagccg acgttccgct cgcgagcggc accccagtga gaccaccagg
     5521 tgtagtggcc cccctccttt tcgaaaagcc ggaacgtatc gacgaagccc gcggcgagca
     5581 tcgcgtcaat cccgatgcgc tcctccttcg tgaaaccctt gtgtccctcg ttctccttag
     5641 ggcgcgcgag gtcgataggc gtgtgggcga cgttgaggtc ccccgcgaag acgaccggtt
     5701 tctcgctatc gagccgtttc atgtactcga gaaacgcggc gtcccaggcg actcgtacgg
     5761 gaatacggga aaggtcgtcc ttcgcgttcg gcgtatagac ggacacgacg tacatattct
     5821 cgaactcgag cgcggtaatt cgcccttctc gtgccgtatc gccatagtcg tccttaaggt
     5881 cgtatttcct cgcgatgtcc tcggggaggc cgaggaggac gttactcggt gccaccttcg
     5941 tgaagatcgc ggtccccgag tacccctttt tctcggaatg gcaccagtat tcgtcatatt
     6001 cggggaggtc gaccgccgcc tcctcctgtt tcgccttcgt ttcctgaagg cagaggacat
     6061 ccggagcgaa ggtctccatg aaccggccga aatcgccctt tttcatgaca gcgcggatgc
     6121 cgttcacgtt ccacgagtag atggtcatgt ggaacagtgt accagtacga gcatataaaa
     6181 aaagcccacc gaacgaatcg gcgggcgaag tcgcgtcgta cccgacaggg aggagtcagg
     6241 cggcggcgcg gatgggaggg tcgatgacgc ggacctcgcc ttcccggagg gagacgatct
     6301 cacgcatcac tgctccgagg ctcggaaagg gctggttctc ggcgaagtag agaacggcat
     6361 ggccctgctc cttcgcgaag ttcgcgatgt ggccgaaacg gctctcgggc aggttcttcc
     6421 agacgtcgat gtcgaggaca tcgacgcagt agttcggcac ttccgaaata tgaccgtgct
     6481 tccgcaggtt ctcgagaatg aacttcgccg acggcgtcat ccgaacctga tccttgtcgt
     6541 agatcatctc gggcacttcg ctccctggcg cgtccttctc gcactcgatg acgaagacta
     6601 cgccgcggac gaggcccgac tcgagaagtt ctcgcgcggg tacgatgaca tccacgtaac
     6661 agcgctcgcg attgaacgac tcgtgggtgg cgcccgactg cgtcgcgaag aacacgacgc
     6721 acggtttctg actgtgagac ggtttgtcgc cttgcatggg tcctccttgg gaacgggcgt
     6781 gcgtcgagtt aaagaacgaa aatgcgaccc acactctata gcggggcggc ttccgctgtc
     6841 aaacggcttt ctctagtgta gaactccgcg aatgaagatt cggttaaaca aaaacccgtc
     6901 catagtgatg aaacgggttt ctgccgttac gcaacggcat tctgcactga aacgaggcgc
     6961 acttcgctct tttttagcgt cctgcgctcg tggaggagca ccgaatgatg cgggatggcg
     7021 ccccggtcga tgacgagaag agcgtccgca ccgcacgttt ccgcgcgaat ccgacacgac
     7081 tccatgctct cggcgaggct gtcaccgggc tggacaagcg agatgtcctc ccggtagagc
     7141 gcttgctgga cgacgcgaag attcttcaaa tgcgtttctg tcggacccgg tgctacgtct
     7201 tccctctgaa gcacgatgat gcactggtca acgacggact gcctaaggat ctcgagaacg
     7261 ggctcgacaa gctccctgtc gcaccgagcc gcgctaaacg gcgaatcggc ttgaggcagg
     7321 atgatggcga gcgccggccg ccttgaatgt tccgtcatac cgcctccctc acatgtaaag
     7381 aactctctgc gtatcgtacg caggccgtca acgctgtcaa catacgattc gcgtggcttc
     7441 atggccgccc ggtctataat aggtacgatt atcagtaagc cgcacaccat ggcaaagggc
     7501 gtcactcatc gcaaggagaa gaaaaagccg aaaaaggcta aaaaatagcg aagttcctcc
     7561 tcacggagga actttttcgt tcaagagcga tgttttgaca tgacgcattt ggcatgtccg
     7621 tgcggtggaa agcacggttg caaacgaccg aggagggcat actatattcc tatggaccat
     7681 tttttggaga agcactgggc gtactacgta aaggacaatc ctgagggcta ctggttcaag
     7741 cggaagctct acgggtgggg gtggacaccg gtcaccaaag aggggtggac cgtcatggcg
     7801 gggtggctca ttctcgtcat cctcaactcg ttccgtctcg agaagacggc gagcgcagat
     7861 ttcgcgaact ggttcgtcct cgagaccctc gcgctctcgc tcatcctcct cgtcatctgc
     7921 tacaaaaagg gtgagaaacc tcgatggcag tggggtctct cgaaggacca actccgcgac
     7981 taaaccgtca cgcttgctcg gttttcttcg cctaccgaac gtggtaggct atgtgcacaa
     8041 ttgtcagtaa cgttttttca tcgcatctat ggataacgca caggagggcg gtgtgcccga
     8101 gaacaccacg aacgagacgc caagcgtccc cgtaacggga acggaggccg ccgcgacgac
     8161 cgccgatgag gcatcgatac cggccgcacc ggtcaccgag cccgcgcgtc gacgcttctt
     8221 cgagggaaag ggagcgatgg tcggcgccat cgtcgccgcg ctcatcctca tcgcgggtgc
     8281 cggatactac gtatacagcc agtactacgc gaagggcggc atcgtcgccg tcgtgaacgg
     8341 ccgaaacatc taccggagcg aactcgatga gagcatcgcc atggtggggg agaacgcgca
     8401 agcgtacggc atcgacgaga cgagttcgac cgcgaacaag gataaacagg accgcgcgat
     8461 ggaagtcctc gtcgacaacg cgctcctcat cacagcggcc gagaacgccg gcttcgacgc
     8521 agacgacgag acggtgcaaa aatcgtacga cgacctcgtc gcgcagatcg gcggcgaaga
     8581 cgcactgaag agccagatgg ccgccgtcgg cctcaccgag tcccgcctcc gtgacaacat
     8641 ccgcgagcgt gtcctcgtcg gcaagtacat cgagtcagtg aacccgagcg ataccatctc
     8701 ggtctccgac aaggaggtcg acgatttcat cgcgcagttg aaggcgaaca acgtcgaact
     8761 tcccgacgac atgacgacgc ttcgacccca gatcgaggcg gagatcaagg cccagaagca
     8821 gcaacagctc gtcgatgcgc tcatcacgaa gttgaagggc gaagcacaga tagacatgcg
     8881 gatctaggcc cgcacgggtg atgaaaaagg cgcctctcgg cgcccttttt cgtcgcctgc
     8941 gccgtaagaa acctgcgccc gcggcgtcag cgtgaatata tggtagacaa gctgaatcgg
     9001 tacacgaaag accgcccacg cctcaagaag gtcgtcgggt gggcgttcgt cgtggtcggc
     9061 ttcgtcgcgc tcgcgctccc catcgtaccc ggcgctccgc tcgtcttcgt cggcttcgag
     9121 gtcttgggca tccgcttcat attcaccgag cgacttcgcg ggctcatcgg gctcgaccgg
     9181 gcagtacccg aagaagtgcc ggagcggagc ctatgaactt aatataaatt tcataggctc
     9241 ggagtgtata ctatccgcat atgcgaacat cacttatcgt gctcggagcc gtcatcgtcc
     9301 tcttagcgct cggcggcatc gccatgtgga taccgaagga gtgggggaac ccggccgcga
     9361 cgcctcccgc gcccgcgacg acgacgaacg tgggtggaac ggtcgcctgt accatggagg
     9421 cgaagatctg tcccgacggt tcaagcgtcg gacgcgttcc tccttcgtgc gagttcgcgc
     9481 catgtcccac ggcggcgacg agtagcaccg atgtcgtcat catcgacgcg ccccgaccga
     9541 acgcgacggt gacatcgccc gtcacggtga cgggaaaagc ccgcggtaac tggtacttcg
     9601 agggtagttt ccccatcgtc gtcgtcaatt gggacggcct catcatcggc caaggtgtcg
     9661 cgcacgcgga cggcgagtgg atgacgacgg actacgtgcc cttcagcgcg tccatttcgt
     9721 acgcgctcgc gtccgagacg ccgtacgcgc gcggctccat cattctgaag aaggacaatc
     9781 cgtcgggcct ccccgagaac gacgattccc gcgagatccc cgtaacgttc tccgaggtac
     9841 gatgatagag agctacttcc tcgtctttct caagatccac gcggcgacga tactcctcct
     9901 catgccgacg atactctacg cggactcgat cgggctcgcc tggatgcgcg gcgtaacgcc
     9961 ggtcgtatcg aagcgagagc tcgcgttcgt gcaccgcgtc gtcttcgtcg gtctcacgct
    10021 catggtcctc tcgggcgccc tcatggcgta tccgtacctc ggatttctcc tctcggagcg
    10081 aagtttcctc ctcaagatgc cgttcgtcct cgcgctcatc gtaaacggca tcttcatcga
    10141 gcggtcgctc
//
LOCUS       DASTSP010000069         6611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1717813, whole genome shotgun sequence.
ACCESSION   DASTSP010000069 DASTSP010000000
VERSION     DASTSP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717813"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFS75_03135"
     CDS             <1..246
                     /locus_tag="VFS75_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677684.1"
                     /translation="GVIGIESAVKKAGGLWGNHEVALFHLTLVAVATVVFLMVRFGHT
                     GLKRPVVHRRLVYAFFTLYTLAFLSGTWLIYAQFPPT"
     gene            324..2003
                     /locus_tag="VFS75_03140"
     CDS             324..2003
                     /locus_tag="VFS75_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4677685.1"
                     /translation="MTQSQTLEILKMGANVFLTGEPGAGKTYVLNRYIDWLSASGVPV
                     AVTASTGIAATHIGGTTIHSWAAIGTKETLTAYDLDRIAQNEKAVRRIGKAKVLVIDE
                     ISMLDGRVLDMVEAVTRTVRQSGEPFGGLQVIVVGDFFQLPPIAGRGEMSRFAFESRS
                     WEAAKFMTCYLTEQHRQEDELLLGLLSSIRRNDIDESHYTLLGEQTEIAFPDIEPTKL
                     FTHNRDVESMNYERLAELETEARTFQMDARGSKQLIEPLVKNCLSPQTLELRREAMVM
                     CTKNNFEAGYVNGTLGRVVEFDRGDGFPVIETGTGKRLKVMPQSWNIEEDGKVKAEIT
                     QVPLRLAWAITVHKSQGMSLDAAEIDLRNAFTYGQGYVALSRVRSLAGMKVIGLNSQA
                     LLVDPRVVHKDAHFREESEDAEYAWGEMDEDEVEKLQRKFVSERGGTWHDGEAPARTP
                     SSPVRENSVEVTKGLLLRRLSASEIARERKMALSTIWGHIEELAKERAIGPAEVETLI
                     PAGLAWDSVYPRIEKAIEKHGAERLKPIYEALGEAYDYDVIRLGRILYTLK"
     gene            2005..2412
                     /locus_tag="VFS75_03145"
     CDS             2005..2412
                     /locus_tag="VFS75_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4677686.1"
                     /translation="MRSRWFRVRRRGRRPRSRAARELYATHKETARARIHERLAHWSV
                     VRGASYGRVAIRDQRSRWGSCSRERNLNFNFRLAFLPVELLDYVIVHELCHLDEFNHS
                     PDFWALVEASLPDYRLRRRALRAIPIESITSPL"
     gene            2409..3062
                     /gene="truB"
                     /locus_tag="VFS75_03150"
     CDS             2409..3062
                     /gene="truB"
                     /locus_tag="VFS75_03150"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU4677687.1"
                     /translation="MSVRLLLIDKPKGITSFDVIRRLRRELGVRKMGHAGTLDPLATG
                     LLLVGVGEGTKDLTKYVGLDKEYLATVRIGERRTTGDLEGAIVEEKEVTELREEDVCD
                     ALSGMVGTLRLPVSPYSAVKQGGVRLYKRARKAEKRGEVIEDTPLRDMVVHEADFLDM
                     KCESGRAEVRVRFKVGSGTYVRSLGEEIGRRLGYPATLADLRRTKVGDFSVEDARAL"
     gene            complement(3106..3504)
                     /locus_tag="VFS75_03155"
     CDS             complement(3106..3504)
                     /locus_tag="VFS75_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677688.1"
                     /translation="MTKPHLSVIPGGRYDEDPIHADGFISPEHNKLVDGSRYSVIWSM
                     VRGDLDVHAFDTFYRAMEKGLDRKFPSVTIVHKPIDGDAGGMQIYLSVVVREEISMES
                     FGELLSLFRGALSTAGIIRRPILCPIPPEP"
     gene            complement(3606..3827)
                     /locus_tag="VFS75_03160"
     CDS             complement(3606..3827)
                     /locus_tag="VFS75_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677689.1"
                     /translation="MRKLDPEGMVRLKAAEALAGSDVKKVPEAVKRVLDGQGGLPPLT
                     HAPKRAFLSHLIEKQLVAIIAMKRRSAPA"
     gene            complement(3847..4179)
                     /locus_tag="VFS75_03165"
     CDS             complement(3847..4179)
                     /locus_tag="VFS75_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677690.1"
                     /translation="MAKSKSTLDTDGRRRYVIAKTLAGDYVENNIDLARAVEKAVGKV
                     LCGDAGMRAVMGPRRGYLKRELTRKVSESLKRTKRKDKLAVMFGEDQAAAMRRDAKKR
                     EEDEAKRH"
     gene            complement(4285..4734)
                     /locus_tag="VFS75_03170"
     CDS             complement(4285..4734)
                     /locus_tag="VFS75_03170"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU4677691.1"
                     /translation="MLHDDIKGQLKEALKAHDEAKLRTVRSMLTAFTNELVATGKKPQ
                     DTLDDAGVMTVIKRLAKQRKDSIEQFEKGGRADLAAIEKEELTVLESYLPQMMSQDEI
                     RPLAEAKIAELGADKSKMGMVIGSLMKELQGKADGGDVKAVVDSLLK"
     gene            4790..5866
                     /locus_tag="VFS75_03175"
     CDS             4790..5866
                     /locus_tag="VFS75_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU4677692.1"
                     /translation="MQNIEIVILFLLFAIVAILVYVFFLKREEKRDDSGLVLLQERLD
                     RLTRTLDERMGETSDRMYESLERQSETSRKLVQEIRDKVEAQLLGVVKGVTEVSESSK
                     QVFTLAEQLKNLEKVLKNQKQRGNLGEASLELILSNVLPGQYEAQHPFKDGEKVDFVI
                     KTAEGLVPVDAKFPLENYIRLVDEADDVRKEVYRKDFKSDVKKRIDETAKYIRPGEGT
                     LPFAFMFIPAEAIYYDLLNSDVGIGVNSRDLIDYAYQQKNVLIVSPTTFLAYLQTVLF
                     GNQRMKVQEAAKDIIKNVENLSRHMKAYDDYFKKVGNTLNTTVNHYNNAQKELAKIDK
                     DVLRITGDSMDIEQLALERPQAVE"
     gene            complement(5878..6291)
                     /locus_tag="VFS75_03180"
     CDS             complement(5878..6291)
                     /locus_tag="VFS75_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677693.1"
                     /translation="MKRVLVCDDDEKYRKPVRHIAECYPVRLVEAATFAELMAAAESG
                     EPFSLVILDGMLDGVNTSIPFVERVAAGCYPHLEDAMFLLVSGDDSLRKQEYRLLKEN
                     WEKFDPANVLVLEEKGFLFREAYKVFKKLFSNPSP"
     gene            6414..>6611
                     /locus_tag="VFS75_03185"
     CDS             6414..>6611
                     /locus_tag="VFS75_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HEU4677694.1"
                     /translation="MKNGKATTKAKEKKDGTFAIIETGGKQYMVAVGDVVEVELLGNL
                     KEGDKVEFDKVLLVDDGKDATI"
BASE COUNT         1337 a         1990 c         2018 g         1266 t
ORIGIN      
        1 ggcgtcatcg gcatcgagtc ggcggtcaag aaggcgggcg gcctctgggg gaaccacgag
       61 gtcgcgctct tccacctcac gctcgtcgcc gttgcgaccg tcgtgttcct catggtgcgc
      121 ttcggccaca cgggcctcaa acgccccgtc gtccaccgtc gtctcgtgta cgcgttcttc
      181 acgctctaca ccctcgcgtt cctctcgggg acctggctca tctacgccca gttccccccg
      241 acctgaatca ccgcagggcc gatgcgcgac ttgccccgca cacacgtgcg gggttctttt
      301 tgcctctgct atactatctc tctatgacgc agtctcagac gcttgagata ttgaagatgg
      361 gtgcgaacgt ctttttgacc ggagagccgg gggcggggaa gacatacgtg ctgaaccgct
      421 acatcgactg gctttcggcc tccggggtgc ctgtcgccgt gaccgcgtcg accggcatcg
      481 cggcgaccca cataggcgga acgaccatcc actcgtgggc agcgatcggc acgaaggaga
      541 ccttgaccgc gtacgacctc gataggatcg cgcagaacga gaaggcggtc cgtcgcatcg
      601 gaaaggcgaa ggtcctcgtc atcgacgaga tctcgatgct tgatgggcgc gtcctcgaca
      661 tggtcgaggc ggtcacgagg acggtgcgcc aatccggcga acccttcggt ggtctccagg
      721 tcatcgtcgt cggcgacttc ttccaacttc cgccgatcgc gggtcggggc gagatgtccc
      781 gcttcgcgtt cgagtcgcgc tcgtgggagg ccgcgaagtt catgacgtgc tacctcaccg
      841 agcagcaccg ccaagaggac gagctccttc ttggtcttct ctcatcgatt cgccggaacg
      901 acatcgacga aagccactac acgctcctcg gggagcagac ggagatcgcg ttccccgaca
      961 tcgagccgac gaagctcttc acgcacaatc gcgacgtcga gagcatgaac tacgagcgtc
     1021 tcgccgagct cgagaccgag gcgcgcacgt tccaaatgga cgcacgcggc tcgaagcaac
     1081 tcatcgagcc actcgtgaag aattgcctct cgccgcagac actcgagctc cgccgtgagg
     1141 cgatggtcat gtgcacgaag aacaacttcg aggccgggta cgtgaacggc accctggggc
     1201 gcgtcgtcga attcgatcgg ggagacggct tcccggtcat cgagaccgga acggggaagc
     1261 gcctcaaggt gatgccgcag tcgtggaata tagaggaaga cgggaaggtg aaggcggaga
     1321 taacgcaggt gccgcttcgc ctcgcgtggg cgatcaccgt ccacaagagc cagggcatgt
     1381 cgctcgacgc cgcggagata gaccttcgta acgcgttcac gtacgggcag gggtacgtcg
     1441 cgctctcgcg cgtccgctcg ctcgccggta tgaaggtcat cgggctcaat tcgcaggccc
     1501 tcctcgtcga cccgcgcgtc gttcacaagg acgcgcactt cagggaggaa tcggaggacg
     1561 ccgagtacgc gtggggcgag atggacgagg acgaggtcga gaagctccag cgaaagttcg
     1621 tctcggaacg aggcggcaca tggcacgacg gcgaggcgcc cgcgcgaacg ccgagttcac
     1681 cggtccgcga gaactcggtc gaggtgacga agggacttct tcttcgccgt ctttccgcct
     1741 cggagatcgc gcgcgagcgg aagatggcgc tctcgaccat ttgggggcac atcgaggaac
     1801 tcgcgaagga gcgcgcaata gggccggccg aggtcgagac actcataccc gcggggctcg
     1861 cgtgggactc ggtctacccg cgcatcgaga aggccatcga gaagcacggc gcggagcgcc
     1921 tgaagcccat ctacgaggcg ctcggtgaag cgtacgacta cgacgtcatc cggctcggtc
     1981 gcattttgta tacgctcaag tgacatgcgg agtcggtggt ttagggtgag gcggagaggg
     2041 aggcgaccgc ggagcagggc ggcgcgcgaa ctctacgcca cacataagga aacggcgcga
     2101 gcgcggatac atgagcggct cgcgcactgg agcgtcgtcc ggggcgcgtc atacgggcga
     2161 gtcgcgatac gcgatcagcg ctcgcgctgg gggagctgtt ctcgcgagcg gaatctcaat
     2221 ttcaacttcc gccttgcgtt tcttcccgtc gagctcctcg actacgtcat cgtccacgag
     2281 ctctgccacc tcgacgagtt caaccactcg ccggacttct gggccttggt cgaagcatcg
     2341 ctcccggact accggctccg ccgccgtgcg cttcgtgcca ttcccatcga aagcatcacg
     2401 tcgcctctat gagcgtaaga ctcctcctca tcgacaagcc gaagggtatc acctcgttcg
     2461 acgtcatacg gcggctccgc cgcgagctcg gcgtgcggaa gatgggccac gcgggaacgc
     2521 tggacccttt ggcgaccgga ctcctgctcg tgggcgtcgg cgagggaacg aaggacctca
     2581 cgaagtacgt cggcctcgac aaggagtacc tcgcgacggt gcgcatcggc gaacgtcgta
     2641 cgaccggcga cctcgaggga gcgatcgtcg aggagaagga agtgaccgaa cttagagaag
     2701 aggatgtatg cgacgcactc tcgggcatgg tcggcacgct tcgtctcccg gtctccccgt
     2761 actccgcggt gaaacaggga ggggtgcgcc tctacaagcg cgcgcggaag gccgagaaac
     2821 gcggtgaggt catcgaggac acgccgcttc gcgacatggt cgttcacgaa gcggactttt
     2881 tggatatgaa atgcgagagc ggccgcgccg aggttcgcgt gcgctttaag gtcgggagcg
     2941 gcacatacgt gcgttcgctc ggagaggaga tcggtcgtcg tctcggttac ccggcgaccc
     3001 tcgccgacct tcgccgtacg aaggtcggcg acttctcggt cgaggacgcg cgagcgctat
     3061 aaaaaagaaa aggcgtccca ttggggacgc ctccgcaaaa gccgctcacg gctccggcgg
     3121 aatggggcag aggatcggcc ggcggatgat gccggcggtc gacagcgcgc ccctgaagag
     3181 cgagagaagc tccccgaacg actccatgga gatctcctcg cgaaccacga cgcttaggta
     3241 gatctgcatg ccgcccgcgt ctccatcgat cggcttgtgg acgatcgtca cggacgggaa
     3301 cttcctgtcg aggccctttt ccatcgcgcg gtagaacgtg tcgaaggcgt ggacgtcgag
     3361 gtcgccgcgg accatgctcc agatgacgct gtagcggctg ccgtccacga gcttgttgtg
     3421 ctcgggactg atgaacccgt ccgcatggat agggtcctcg tcgtaccgcc caccggggat
     3481 aacgctcaaa tgcggttttg tcacttggct ctcctccgtt taaaccagcc gatatactgc
     3541 aataggaaat tcatatagtc aagcatagaa aaaggggcgc tctagagcgc ccccgggaac
     3601 cgctcctaag cgggagcgga tcgtcgcttc atggcgatga tcgccacgag ctgcttctcg
     3661 atgaggtgtg agaggaatgc ccgcttgggg gcatgcgtga gaggcggtag tccgccttgt
     3721 ccgtcgagaa ctctcttcac cgcctcgggg accttcttca catcacttcc ggcgagcgct
     3781 tcggccgcct tgagacggac catgccctcg gggtcgagct ttctcattct ccctccgtat
     3841 agaacgtcag tggcgtttcg cctcgtcctc ttcgcgcttc ttcgcgtcgc gacgcatcgc
     3901 tgccgcttgg tcttctccga acatgaccgc gagcttgtcc ttccgcttcg tccgcttgag
     3961 actttccgag accttgcgcg tgagttcccg tttcaggtat ccacgccgcg gtcccatgac
     4021 cgctcgcatg cccgcgtcgc cgcagagcac cttgccgacc gccttctcga ccgccctcgc
     4081 gaggtcgatg ttattctcga cgtagtcgcc cgcgagcgtc ttcgcgatca cgtaccgccg
     4141 ccttccatcc gtatcgagcg tgcttttgga cttcgccatc gcaaactcct caagatagaa
     4201 acccgcgaag attatgcaac acattggtat tgtgcgcaag tgacaccacg gcacgaaaaa
     4261 accgcccaaa aggcggcctc tcgtctattt gagaagcgaa tcgacgaccg ccttcacgtc
     4321 gccaccgtcc gcctttccct gaagctcctt catgagggaa ccgatgacca tgcccatctt
     4381 cgacttgtcg gcgccgagtt cggcgatctt cgcctccgcg agcgggcgga tctcatcttg
     4441 tgacatcatc tgggggaggt acgactcgag gacggtgagc tcctccttct cgatagccgc
     4501 gaggtcggcg cggcctcctt tctcgaactg ctcgatcgag tcctttcgtt gcttcgcgag
     4561 gcgtttgatg accgtcatga cgcccgcgtc gtcgagcgtg tcctgcggct tcttgccggt
     4621 cgcgacgagc tcgttcgtga atgcggtgag catgctccgg accgtgcgga gcttcgcctc
     4681 gtcgtgcgct ttgagggcct ctttgagttg tcccttgatg tcgtcgtgta gcatacgtgt
     4741 tgggttagtg gtttctatag taccacagcg ggcgtggtag aatagggcca tgcagaacat
     4801 cgaaatcgtt attcttttcc tcctttttgc catcgtcgcc atcctcgtgt acgtcttttt
     4861 cctcaagcgc gaggagaagc gcgacgactc cggcctcgtc ctcctccaag agcggcttga
     4921 tagactcact cgaacgctcg acgaacgcat gggagagacg agcgaccgca tgtacgaatc
     4981 cctcgagcgt cagtcggaga cgagcaggaa acttgtccag gagatcaggg acaaggtgga
     5041 ggcgcagctt ctcggcgtcg taaagggagt gacggaggta tccgaatcgt ctaagcaggt
     5101 ctttactctc gctgagcagc ttaagaacct tgagaaggta ttgaagaatc aaaaacagcg
     5161 ggggaatctc ggtgaagcgt ccctcgagct catactctcg aacgtactcc cgggacagta
     5221 cgaggcgcag catccgttca aagatggcga aaaagttgac ttcgtcatca agacggccga
     5281 aggcctcgtg cccgtcgatg cgaaattccc gctcgagaac tacatacgat tggtagacga
     5341 ggcggacgac gtgcgaaagg aagtgtaccg caaagatttc aagtcggacg tgaaaaaaag
     5401 gatcgacgag accgcgaagt acatcaggcc gggagaggga acactcccgt tcgcattcat
     5461 gttcattccc gcggaggcga tctactacga cttactgaac agtgacgtgg gcatcggcgt
     5521 caactcgcgc gacctgatcg actatgccta ccagcagaag aacgttctca tcgtgtcgcc
     5581 gaccacgttc ctcgcgtacc tccagacggt gctcttcgga aaccagcgca tgaaagtcca
     5641 ggaggcggca aaagacatca tcaagaacgt ggagaacttg agtcgccaca tgaaggcata
     5701 cgacgactac ttcaagaaag tgggcaatac cctgaacacg accgtgaatc actataataa
     5761 cgcccagaag gaattggcga agatcgacaa agacgtcctc cgcatcaccg gcgattccat
     5821 ggacatcgaa cagctcgctc tcgaacgtcc gcaggccgtc gagtaaggaa aaacccgtca
     5881 cggggacggg ttcgaaaaga gctttttgaa gaccttgtag gcctcacgga agaggaatcc
     5941 tttttcttcg agcacgagga cgttcgcggg gtcgaatttc tcccagttct ccttgaggag
     6001 cctatactcc tgctttcgga gcgaatcgtc tcccgagacg aggaggaaca tcgcgtcttc
     6061 gagatgcggg tagcacccgg ctgcgacgcg ttccacgaag gggatgctcg tattcacccc
     6121 gtcgagcatg ccatcgagga taacgagcga gaacggctcg ccgctctcgg ccgctgccat
     6181 gagctcggcg aacgtcgcag cctcgacgag ccgcacggga tagcattcgg cgatgtgccg
     6241 gaccggcttt cggtacttct catcgtcatc gcagacgaga acgcgtttca ttgggtctct
     6301 ccaaggtatg tcactctgcg gataatgcga cacggcgacc cttttggcaa gtccttccga
     6361 aagaggcgaa actattgcat attttggcgc tttcttgtac aatcggcggc attatgaaaa
     6421 acgggaaagc gacaaccaaa gcaaaggaga aaaaggacgg taccttcgcc atcatcgaga
     6481 ccggcggcaa gcagtacatg gtcgcggtcg gcgatgtcgt cgaggttgag cttctcggaa
     6541 atctcaagga gggggacaag gtcgagttcg acaaggttct cctcgtcgac gacggcaagg
     6601 acgcgacgat c
//
LOCUS       DASTSP010000070        12385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1719980, whole genome shotgun sequence.
ACCESSION   DASTSP010000070 DASTSP010000000
VERSION     DASTSP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12385
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719980"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFS75_03190"
     CDS             complement(<1..304)
                     /locus_tag="VFS75_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677695.1"
                     /translation="MRILFISNELGGSGLCQKLIEEGNDLKLYIHRPEWQACYQGLAA
                     RVANWRHELNWVGKDGLIIFDDVFFGREQGELRKDGYKVVGGSEGGDALELDRRHFQ"
     gene            complement(426..1343)
                     /locus_tag="VFS75_03195"
     CDS             complement(426..1343)
                     /locus_tag="VFS75_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4677696.1"
                     /translation="MDRSWFLLAVLSPFLWSMCNHIDKIVLEKYFKEGGVGALIIVSA
                     LASIIAVPFLYIMDPTVLEIEPGTAWVIVLTAVLDVVLLWAYLSALQKDESTNVILYY
                     QLVPIFGLGTGYLFLGETVSSGQLAAMAIVILGTSIVSLETTEGKFCFKKRTVGFMLL
                     ACAAWAAELAIFKVVAIEDNAWRTLFWKHIVLTGIGVVIFAFVPRYRRNFLGAMRSNS
                     VPILGLNLLNEVLYMLGAIAYGLAVMHAPVGVVLLTETFQSIFVFVLGLLIAAFLPKL
                     ATESTDIGNVMRKVVAIGVTGLGTYLLLV"
     gene            complement(1449..1925)
                     /locus_tag="VFS75_03200"
     CDS             complement(1449..1925)
                     /locus_tag="VFS75_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677697.1"
                     /translation="MDTAHIVALLRESIFMIATLVVFLVYAMAKGRYALINLILALYL
                     ALLVSVEFPYYRQLSGSGEGSAVLKILVFLAFVVVGVVLMRRHIPGDDFEAAFHGLGK
                     KLLLAALATILVMAFSFHALPVTEIIHPGTPIQALFAPSDRFFWWLAIPLGVLLFI"
     gene            complement(1981..2541)
                     /locus_tag="VFS75_03205"
     CDS             complement(1981..2541)
                     /locus_tag="VFS75_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677698.1"
                     /translation="MNKRKKHLLFDVALFFIGIAAAYAAAKYGAVAAFVALLDGYKLV
                     AAFIAGLLFTSVLTTAIGIVIFVKLGATMPLLAVALVGGLGAMAIDYVMFWFTRDRIM
                     ADYEYMLANASGRIRALFTLCKQCLLRRLLPIIGAFIIMSPFPDELGVALMGFSNISI
                     RKFLALTYVMNAAGILVVVLLGDSLF"
     gene            complement(2836..3909)
                     /gene="dnaJ"
                     /locus_tag="VFS75_03210"
     CDS             complement(2836..3909)
                     /gene="dnaJ"
                     /locus_tag="VFS75_03210"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU4677699.1"
                     /translation="MKDYYDILGIDKSASKDDVKRAFRKLAAKYHPDKKTGDEAKFKE
                     ISEAYSVLGDDKKRAEYDAYGRSYAGGQGGGFGGQWGGFQGGFGQGGVEFDLNDIFEN
                     FGDIFGGGVRGSRRQARGHDISVDIELSFKEAVFGTTRTLRITKNSTCPTCKGSGAKP
                     GTDMTTCAVCNGNGRVRETRQSILGQFATVRECDNCGGTGKVPKEACADCGGEGVKRM
                     EETIEIVVPAGIENGEMIRMTGRGEAVRGGTPGDLYVKVHVERHPTITRNGSTLESSL
                     RVKLSDALLGASYGVETLDGTITIKIPEGVKHGEVLRIKGKGVPVSGSSRGDFHVKIL
                     IDIPQKLSRNAKKLIEELKKEGI"
     gene            complement(3949..5847)
                     /gene="dnaK"
                     /locus_tag="VFS75_03215"
     CDS             complement(3949..5847)
                     /gene="dnaK"
                     /locus_tag="VFS75_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU4677700.1"
                     /translation="MAKILGIDLGTTNSAMAIIEGGEPTIIENAEGNRTTPSIVAISK
                     TGERLVGQIAKRQSVTNPKNTIYGIKRFMGHNFSEPAVQSDKSIVPYEVREGQDGGVL
                     IHMGDKDYRPEEISAMILSKLKQDAEAKLGEKITEAVITVPAYFNDSQRKATQDAGKI
                     AGLDVKRIINEPTAAALAYGFNKKKEEKIVVYDFGGGTFDVTVLEVGDDVIEVKSTDG
                     DAHMGGRDIDQRVVRYLIDEFKRSEGIDLSKDPLALQRLDEAAEKAKHELSTMGEAEI
                     NIPFITSDQNGPKHMLLRLTRAKLEELAAEFIDRSIEITKRALEASSLKKEDIDEIIL
                     VGGQTRMPKIVERVRELFGKEPNKSVNPDEVVALGAAIQAGIFQGDIHDITLVDVIPL
                     SLGIETMGGVSTKLIEKNTHIPTKREQVFSTAADNQTSVEIHITQGERPMAADNKSLG
                     RFILDGIPPAPRGIPQVEVAFDVDSNGVLNVTAKEKSTGKAQQIRIEANSGLSADDIE
                     RMKQEAEAHAAEDEKKKELIEARNLADQMLYTAEKSLTDYKDKVSADVKTAVEDAVKA
                     LREVKDKDDIAAIKAKTDELSSAMSKIGEAMQKAGANASETSSEAPKDGEGPIRDAET
                     KDDSDKKE"
     gene            complement(5889..6470)
                     /locus_tag="VFS75_03220"
     CDS             complement(5889..6470)
                     /locus_tag="VFS75_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU4677701.1"
                     /translation="MAEEKNLENEPELVEDDEIVTSEGDDDDSVDTAARGVVKRLREK
                     LKASEEERRKALEDLQRARADFLNSKRRIEEQLVRDRERAADGILIELLSVLDSFDTA
                     TADEALWNAVDEKWRAGMEAIHANLRSILRRSGVTEIESLGAHFDPAKHEAVSNIAVT
                     DPEKTDTIISVLQKGYRRGDTILRPAKVVVGTA"
     gene            complement(6775..7173)
                     /gene="rpsI"
                     /locus_tag="VFS75_03225"
     CDS             complement(6775..7173)
                     /gene="rpsI"
                     /locus_tag="VFS75_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU4677702.1"
                     /translation="MEKTYTEGIGRRKTATARVRIVKATAQRVVINGRPMAEYFKAPA
                     LQRNILGVLETENAGIDTYEISAKVEGGGLSAQADAIKLGVARALVKEKADRRVPLKR
                     AGYLKRDARAVERKKFGLRKARRRPQWSKR"
     gene            complement(7186..7536)
                     /locus_tag="VFS75_03230"
     CDS             complement(7186..7536)
                     /locus_tag="VFS75_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HEU4677703.1"
                     /translation="MDKKEYTIDAEGKRLGRVATEAASVLLGKNTTHFTKHTVEPVVV
                     KIVNASKLDISDKRATEEFQSYSGFPGGRRVETLGHLATRLGYGEVVRRVVAGMLPKN
                     KLHTPRMHNLEVTE"
     gene            complement(7542..7895)
                     /gene="rplQ"
                     /locus_tag="VFS75_03235"
     CDS             complement(7542..7895)
                     /gene="rplQ"
                     /locus_tag="VFS75_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU4677704.1"
                     /translation="MKHHVKNRTFGRPKNQRTALLRGLAMSLLTHGKITTTEAKAKEL
                     RPYVEKLITKGKENTMTARKLVSSRLGEPDPKVVKRIFDDVAKRYADRNGGYTRIIKL
                     GQTSAGRTEACIELV"
     gene            complement(7899..8864)
                     /locus_tag="VFS75_03240"
     CDS             complement(7899..8864)
                     /locus_tag="VFS75_03240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU4677705.1"
                     /translation="MLETNVTLPSKPRVIKEEEFSGVFEIDGLYPGYGHTLGNSLRRI
                     ILSSLPGAAITQVKIDGVDHEFSTLAGVKEDVITILLNLKRVRLQMHGDTPLTIALKA
                     SGEKLVTAGDIDAPSQITILNPEQRIAEITAKGTTLNIELTVEHGLGYVPRDVHQKEK
                     VDIGTIALDAVFTPIRRVNYEVENMRVGDRTDYNRLRVFIETDGTLTPREALEQSIEI
                     MIHQLKAIIGFQDTEPKREPAPSEPVPAEGEPESVDADILKTRIETLDLSTRTQNALE
                     NANIRTLGGLVRKKKEDILELEGIGPKGVDELETILKGMNLGFKE"
     gene            complement(8894..9505)
                     /gene="rpsD"
                     /locus_tag="VFS75_03245"
     CDS             complement(8894..9505)
                     /gene="rpsD"
                     /locus_tag="VFS75_03245"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU4677706.1"
                     /translation="MKIGPKYKIAKRLGAAVFEKTQTQKFALSEARSARNKKMRRGGM
                     TSDYKKQLIEKQKMRFLYGISEKQLSNYVRDAVAAGTQPVLRLNERLESRLDNVVYRL
                     GLAKTRRLARQLVSHGHIQVNGRRFNIPSYRVKIGDTVSVREQSKQSPLFVTLGETLA
                     SATVPAWLTLDAKKLSGEMKAEPSYEPAETIFDVQQVLEYYSR"
     gene            complement(9515..9940)
                     /gene="rpsK"
                     /locus_tag="VFS75_03250"
     CDS             complement(9515..9940)
                     /gene="rpsK"
                     /locus_tag="VFS75_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU4677707.1"
                     /translation="MGKKRIIKKGGDSSVTGTALTRVPKKKVASGVLNVLATFNNTML
                     TLCDTKGNSLITVSSGALGFKGSRKSTPFAAAKVGEMIGDKAAQMGMKDASVVIRGIG
                     AGRESALRGFSSKGIAITKIADQTPVPHNGPRLPKPRRI"
     gene            complement(9974..10351)
                     /gene="rpsM"
                     /locus_tag="VFS75_03255"
     CDS             complement(9974..10351)
                     /gene="rpsM"
                     /locus_tag="VFS75_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU4677708.1"
                     /translation="MRISGITIPDTKHLEYGLTAVYGIGHSRAVSILKSLSIDPMKSA
                     KDLTTDEENAIREKVESFTIEGELKREVTGNIKRLKDIKSYRGSRHAKRLPARGQRTK
                     TNARTLKGVKKTMGSGRRKLEKT"
     gene            complement(10354..10470)
                     /gene="rpmJ"
                     /locus_tag="VFS75_03260"
     CDS             complement(10354..10470)
                     /gene="rpmJ"
                     /locus_tag="VFS75_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU4677709.1"
                     /translation="MKIRSSVKKISPDDKIVRRRGYVYVINKKKPRNKQRQG"
     gene            complement(10480..10701)
                     /gene="infA"
                     /locus_tag="VFS75_03265"
     CDS             complement(10480..10701)
                     /gene="infA"
                     /locus_tag="VFS75_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001872.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU4677710.1"
                     /translation="MQEQSDKDNLVYGTVEEALPNALFRVLLDGEEKPILAYLAGKMR
                     KFKIRVLVGDRVALVPEPYGGKARITKRL"
     gene            complement(10784..12121)
                     /locus_tag="VFS75_03270"
     CDS             complement(10784..12121)
                     /locus_tag="VFS75_03270"
                     /inference="COORDINATES: protein motif:HMM:NF044707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677711.1"
                     /translation="MAKKWNLQDIRPAGARKPVAPRPAESRKARMDVAPRPAEPEEPP
                     RFDDSDIERLDVVDGNGARKKRIITTSVIAVVVLALGFAVNVMLSGADVTVYPKTRDV
                     FVQADFTAKKTPQAGELGYELLSLEATGEKQVKATGKENVATQAEGKIIVYNSKSTLP
                     QRLIKNTRFESPDGLIYRIKESIEVPGVTKDAKGNEVPGTVVATVFADQPGEKYNIGP
                     TKFTVPGLKGTDQYDSVYGESTIAFTGGFEGEKYIIDPAELQTAEQALQLELRDTLLA
                     RLKEERPAGFIIYDDSIAVSFESLPSTEYGDSLATIKEKAKLQVPMFKESEFASFIAK
                     QSVPDYAGDAVSIPNPESLAFTYTSATTTVSDISTLDSLEFLLKGNARLIWTFDENKL
                     KNDLKGKKKTEATAVFGSYPAIANAQAEVRPFWEGSFPDDSNDIAVTTITDKK"
     gene            complement(12145..>12385)
                     /locus_tag="VFS75_03275"
     CDS             complement(12145..>12385)
                     /locus_tag="VFS75_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677712.1"
                     /translation="IVEKRAFPMHVILAAQYPFTNLFTHAVTIGIRSVIAKKPTVTAI
                     ERRLVEEVGGRKSADTYLALEARFFHKYDAGEKRV"
BASE COUNT         2180 a         3688 c         3723 g         2794 t
ORIGIN      
        1 cctgaaagtg tcgccgatcg agttcgagcg catctccgcc ctcgctcccc ccgactacct
       61 tgtagccgtc tttccggagc tcaccctgct ccctgccgaa aaaaacatcg tcgaagatga
      121 tgagcccatc ctttcctacc caattaagtt catgtctcca gtttgcgaca cgcgccgcta
      181 ggccttgata acatgcctgc cactcgggcc tatgaatata aagctttagg tcgttcccct
      241 cctcaatgag tttctgacag agacctgagc cacccagttc gttcgagatg aagagaatac
      301 gcatataaaa aataagccgg cggaccgtga tctcatcata tcgtcattgg gcaggattgt
      361 gcagtaggag gtgggtatga gacgtaaaaa acgcgcagcc gaagctgcgc gttaaaggtc
      421 caccgtcaca cgaggaggag gtacgtgccg agaccggtga caccgatcgc gaccaccttc
      481 cgcatcacgt ttccgatgtc ggtcgactcc gtcgccagtt tcgggagaaa cgcggcgatg
      541 agaagaccga gcacgaagac gaagatggac tggaaggtct ccgtgaggag gacgaccccg
      601 acgggcgcgt gcatgaccgc gaggccgtac gcgatagcgc cgagcatgta cagcacctcg
      661 tttaggaggt tgaggccaag gatcggcacc gagttcgagc gcatcgcgcc gaggaagttc
      721 ctccgatacc gcgggacgaa cgcgaagatg acgacgccga tcccggtaag cacgatgtgc
      781 ttccagaaga gcgtccgcca cgcgttgtcc tcgatcgcga ccaccttgaa gatggcgagc
      841 tccgcggccc atgcggcgca tgcgagaagc atgaacccca cggtcctttt cttgaagcag
      901 aacttcccct ccgtcgtctc gagcgagacg atcgaggtgc cgaggatgac gatcgccatc
      961 gcggcaagct gccctgacga gaccgtctcg ccgaggaaca ggtaacccgt cccgaggccg
     1021 aagatcggca cgagttggta gtacaggatg acgttcgtcg actcgtcctt ctggagggcg
     1081 gaaaggtatg cccagaggag gacgacgtcg agtaccgcgg tcaaaacgat gacccatgcc
     1141 gtacccggct cgatctcaag gaccgtgggg tccatgatat agagaaaggg tacggcgatg
     1201 atcgatgcga gcgcgctcac aatgatgagc gcgcctacgc cgccctcctt gaaatacttc
     1261 tcaaggacga tcttgtcgat gtgattgcac atcgaccaaa gaaacggcga gagtaccgca
     1321 agcaggaacc aggagcggtc catgtggtgg ccctcatgtg agttgtggaa gtgctactat
     1381 ttaatatgat agcacaaaaa aagcacattg tctagtttgt gacaatatgc cttgattaag
     1441 ccgtagaatt agatgaaaag gaggacgccg agcgggatgg cgagccacca gaagaatcgg
     1501 tcagagggcg cgaacaaagc ttgaataggc gttccagggt ggatgatctc ggtcaccgga
     1561 agcgcatgaa acgagaacgc catgacgaga atggtcgcaa gcgccgcgag gaggagcttc
     1621 ttcccgagtc catgaaaggc cgcctcgaag tcgtcgccgg ggatgtgccg acgcatgagg
     1681 acgacgccga cgacgacgaa cgcgaggaat acgagtatct taaggaccgc acttccctcc
     1741 ccgctccccg agagctgtcg atagtacggg aactccacgg agacgagaag cgcgaggtac
     1801 agggcgagga tgaggttgat gagcgcatag cgtcccttcg ccatcgcgta gacgagaaag
     1861 acgaccaacg tcgctatcat gaagatcgac tcgcgaagta atgcgacgat gtgcgccgta
     1921 tccatcccct atagtatgcg gctccgcaac gacgtttgga cgacgggatg tgtacagcgg
     1981 ttagaagagc gaatctccga gaaggacgac gactaaaata cccgcggcgt tcatgacgta
     2041 cgtgagcgcg aggaacttcc ggatgctgat gttcgagaat cccatgagcg cgacaccgag
     2101 ctcatcggga aacggcgaca tgatgatgaa cgcgccgatg atgggtagaa gccgacgaag
     2161 gagacactgc ttgcagagcg tgaagagggc gcggattcgc ccgctcgcgt tcgcgagcat
     2221 atactcgtag tccgccatga tgcggtcgcg cgtgaaccag aacatgacgt agtcgatggc
     2281 catcgctccg aggccgccga cgagggcgac tgcgagaagc ggcatcgtcg cgccgagctt
     2341 cacgaagatg acgatgccta tcgcggtcgt gagaaccgac gtgaagagga ggccggcgat
     2401 gaacgcggcg acgagtttgt atccatcaag gagcgcgacg aaggcggcga ccgcgccgta
     2461 cttcgctgcc gcgtaggcgg ccgcgatgcc gatgaagaag agcgcgacgt cgaaaagaag
     2521 gtgcttcttc cgtttgttca tgggagtagt gtaacacgag gtcgtctaat tgaacggaaa
     2581 catcgccgtc ttacccacct cgcggtttcg tctcctcggg gtcgacgaaa catcgctccg
     2641 gttcatctga ctaaaaaggc gtccgaaggg aagccttttt aacgcaaggg tcaagttgct
     2701 aagcgaaata ctttcactcc gttcgtattt cgctaagcac ttcctccccg ggctcgttcg
     2761 tgctcgcaat ttgcgggcac atcgctccgc ctcatataaa ctaaccgaat atccgcaggg
     2821 atgttcgttt tacgtttaaa tgccttcttt cttcagttcc tcgatgagct tcttcgcgtt
     2881 tcgagagagc ttctgcggga tgtcgatgag tatcttcacg tggaagtcac ctcgagacga
     2941 gccggagacg ggcacgccct tccccttgat gcggagaacc tcgccgtgct tcaccccttc
     3001 ggggatcttt atcgtaatgg tgccgtcgag cgtctcgacg ccgtagctcg cgccgaggag
     3061 cgcgtcggag agcttcacgc ggagcgacga ctcgagcgtg ctcccgtttc tcgtaatggt
     3121 cgggtggcgt tcgacgtgga ccttcacgta gaggtcgccc ggcgtaccgc cgcgaaccgc
     3181 ctcgccgcgc cccgtcatgc gtatcatctc gccgttctcg atgccggccg gaacgacgat
     3241 ctcgatggtc tcctccatgc gcttcacgcc ttccccgccg cagtccgcgc acgcctcctt
     3301 cggcaccttc ccggtgccgc cgcagttgtc gcattcgcgt accgtcgcga actggccgag
     3361 gatgctctgc cgcgtctcgc ggactcgccc gttcccgtta cagacggcgc acgtggtcat
     3421 atcggtaccg ggcttcgcac cggagccttt gcacgtcgga cacgtcgagt tcttcgtgat
     3481 gcggagcgtt cgcgtcgtac cgaagaccgc ctccttgaag gagagttcga tgtcgaccga
     3541 gatgtcgtgg ccgcgggctt gcctccgcga accccggaca ccgccaccga agatatcgcc
     3601 gaagttctcg aagatgtcat tgaggtcgaa ctcgacgcct ccctgaccga agccgccctg
     3661 gaacccgccc cactgcccgc cgaagccacc tccctggccg ccggcgtagg agcgtccgta
     3721 cgcatcgtac tcggcgcgct tcttgtcatc gccgaggacg gagtaggcct cgctgatctc
     3781 cttgaacttc gcctcgtccc cggtcttctt gtcggggtgg tacttcgcgg cgagcttgcg
     3841 gaacgcgcgc ttcacgtcgt ccttcgaagc gctcttatcg ataccgagga tgtcgtagta
     3901 gtctttcata cgcgtagtgt actcctataa cctcgagtcg aacagtggtc actccttctt
     3961 gtcagaatcg tccttcgttt ccgcgtcacg gatcgggcct tcgccgtcct ttggcgcctc
     4021 ggacgacgtt tcgctcgcgt ttgcgccagc cttttgcatc gcctcgccta tcttcgacat
     4081 cgcgctcgag agttcgtcgg tcttcgcttt gatcgcggcg atatcgtcct tgtccttcac
     4141 ctcgcggagc gccttcaccg cgtcttcgac ggcggtcttc acgtcggcgg aaaccttatc
     4201 cttgtagtcc gtgagcgact tctccgcggt atagagcatc tggtcggcga gattgcgcgc
     4261 ttcgatgagc tccttcttct tctcgtcctc cgcggcgtgc gcctccgctt cctgcttcat
     4321 gcgctcgatg tcgtccgcgg agagtccgga gttcgcctcg atgcggatct gctgggcctt
     4381 ccccgtcgac ttctccttcg cggtcacgtt gaggacgccg ttactgtcca cgtcgaacgc
     4441 gacctcgacc tgcgggatgc cgcgtggtgc aggcggaata ccgtcgagga tgaaccggcc
     4501 aagcgacttg ttgtccgccg ccatcgggcg ctcgccctgc gtgatgtgta tctcgacgga
     4561 cgtttggtta tccgcggcgg tcgaaaagac ctgttcgcgc ttcgtcggga tgtgcgtgtt
     4621 cttctcgatg agcttcgtcg agacgccgcc catggtctcg ataccgagag agagcggaat
     4681 gacgtctacg agcgtaatgt cgtggatgtc gccctggaag atacccgctt ggatcgcggc
     4741 gccgagggcg acgacctcat ccgggttcac cgacttgttc ggctcctttc cgaagagctc
     4801 gcgtacgcgc tcgacgatct tcggcatgcg ggtctggccg ccgacgagaa tgatctcgtc
     4861 gatgtcctcc ttcttaagcg aagacgcctc gagcgcgcgc ttcgtgatct cgatggagcg
     4921 gtcgatgaac tccgccgcga gctcttcgag cttcgcgcgc gtgaggcgaa ggagcatgtg
     4981 cttggggccg ttctggtcag acgttatgaa cgggatgttt atctccgcct cgcccatcgt
     5041 cgagagctcg tgcttcgcct tctccgcggc ctcgtcgagg cgctggagcg ccaaggggtc
     5101 cttcgagagg tcgataccct ccgatctctt gaattcgtcg atgaggtagc gaacgacgcg
     5161 ctggtcgatg tcgcggccgc ccatgtgcgc gtcgccgtcg gtcgacttca cctcgatgac
     5221 gtcatcgccc acctcgagga cggtcacgtc gaacgtgccg ccgccgaagt cgtacacgac
     5281 gatcttctcc tctttcttct tattgaatcc gtacgcgagc gctgccgcgg tcggctcgtt
     5341 gatgatgcgc ttcacgtcga ggccggcgat ctttcccgcg tcctgcgtcg ccttacgctg
     5401 actgtcgttg aagtacgcgg gtacggtgat gaccgcttcc gtgatcttct cgccgagctt
     5461 cgcctcggcg tcctgcttca gcttcgagag gatcatcgcg gatatctcct cggggcggta
     5521 gtccttgtcg cccatgtgaa tgagaacgcc accgtcctgc ccttccctca cctcataggg
     5581 gacgatggac ttgtcgctct gcaccgcggg ttcgctgaaa ttgtggccca tgaaccgctt
     5641 gatgccgtag atggtgttct tcgggttcgt gaccgactga cgcttcgcga tctggccgac
     5701 gaggcgctcg cccgtcttcg atatcgccac gatcgagggc gtcgttcggt tcccttccgc
     5761 gttctcgatg atcgtcggct cgccgccttc gatgatcgcc atcgcggagt tcgtcgtacc
     5821 gaggtcaatg ccgagtatct ttgccatatg ttttctacgt tatgtcgata atggattaaa
     5881 aaaggatgct acgcggtacc cacgaccacc ttcgccggtc ggagtatcgt atcgccgcgc
     5941 cggtagccct tctggaggac cgagatgatg gtatccgtct tctccgggtc ggtgacggcg
     6001 atgttcgaga ccgcttcgtg cttcgctggg tcgaagtgcg cgccgagcga ctcgatctcc
     6061 gtgacgccac tgcggcggag tatggaccgc aggttcgcgt gaatcgcttc catgcccgcg
     6121 cgccacttct cgtcgacggc gttccagagc gcctcatcgg cggtcgcggt gtcgaagctg
     6181 tcgaggacgc tgagaagctc gatgaggatg ccgtccgccg cgcgttcgcg gtcgcggacg
     6241 agctgttcct cgatgcgtcg cttgctattg agaaagtccg ctcgcgctcg ctgaaggtct
     6301 tcgagcgcct ttctacgctc ctcctcggac gctttgagct tctcgcgaag tcgcttgacg
     6361 accccgcgag ccgccgtgtc gaccgaatca tcgtcatcgc cttcgctcgt cacgatttcg
     6421 tcatcttcga ccagctctgg ctcattttca aggttttttt cctctgccat agggacgcat
     6481 tatacagacg gattttctcc ctgtcaaaat tgggtcgact cccccgtatt cgaagtatac
     6541 gcttcgatta tgtatgggag attaacgtgc tccgcgtgtc caaacgatac ccttctatga
     6601 aaatgtcacc gtgaggtggc attttcagga gattaatgct tgccgtcagg tcactaactc
     6661 gcttactctc ataagtgcct cctggccgga ctcatggttt tgcctccttg cggtcggcaa
     6721 aacatcattc cgtcttcgaa gcgctgaaaa aggctccgga ggggagcctt tttgctaacg
     6781 cttcgaccac tgtgggcggc gtcgggcctt tcggaggccg aacttcttgc gttcgaccgc
     6841 gcgagcgtcc cgcttcaggt atcctgcgcg cttaagcggt acgcggcggt cagctttctc
     6901 cttcacgaga gcgcgagcga caccgagctt gatggcgtcc gcctgcgcgg agagtccgcc
     6961 gccctcgacc ttcgccgaga tctcgtacgt gtcgatgccg gcgttctcgg tctcgaggac
     7021 tccgagaata tttctctgga gcgcgggcgc cttgaagtac tccgccatgg ggcgtccgtt
     7081 aatgacgacg cgctgtgccg tcgccttcac gatgcgaacg cgcgcggtcg cggtctttcg
     7141 tcgtccgatg ccctctgtgt atgtcttttc catgataatg atgcgttatt ccgttacctc
     7201 gaggttatgc atgcgcggcg tatggagctt gttcttcgga agcatgccgg cgacgacgcg
     7261 tcggacgacc tcgccgtagc cgaggcgcgt cgcgaggtga ccaagggtct cgacgcggcg
     7321 gccgcccggg aagccggagt agctctggaa ctcctccgtt gctcgcttat cggagatgtc
     7381 gagcttcgag gcgttcacga tcttcacgac gaccggctcg accgtgtgct tcgtgaagtg
     7441 cgtggtgttc ttgccgagga gcacggatgc ggcctccgtc gcgactcgtc cgagtcgctt
     7501 gccttcagcg tcgatggtgt attctttctt atccatatgc gttatacgag ctcaatgcaa
     7561 gcctccgtgc gcccggccga cgtctgtccg agcttgataa tgcgcgtgta gccaccgttt
     7621 cggtcggcat accgcttcgc gacgtcgtcg aatatcctct tcacgacctt cgggtcgggc
     7681 tcgccgaggc gagacgagac gagcttacgc gccgtcatcg tgttctcctt gcccttcgtg
     7741 atgagcttct cgacgtacgg tcgaagctcc ttcgccttcg cctcggtcgt cgttatcttg
     7801 ccgtgcgtga ggagggacat cgcaagaccg cgaagaagcg cagtgcgctg attcttcggc
     7861 cggccgaacg tcctgttttt aacgtgatgc ttcatacgct attccttaaa tccgaggttc
     7921 atgcccttaa gtatcgtctc gagctcatcg acgcccttcg gaccgatgcc ctcgagctcg
     7981 aggatgtcct ccttcttctt tcgaacgaga ccaccgaggg ttctgatgtt cgcgttctcg
     8041 agggcgttct gcgttcgggt cgaaaggtcg agggtctcga tacgcgtctt caagatatcc
     8101 gcgtcgacgg actcaggctc gccttcggcc gggaccggct ccgacggagc gggctcgcgc
     8161 ttgggttcgg tgtcctggaa gccgatgatg gctttgagct gatggatcat gatctcgatc
     8221 gactgctcga gcgcctctcg cggcgtgagc gtaccgtcgg tctcgatgaa gacgcggagg
     8281 cggttgtagt cggttcggtc gccgacgcgc atgttctcga cctcgtagtt cacacggcgg
     8341 atcggcgtga agaccgcgtc gagcgcgatg gtaccgatgt cgaccttctc cttctggtgt
     8401 acgtcgcgag gaacgtatcc gaggccatgc tccacggtga gctcgatgtt gagcgtcgtt
     8461 cccttcgcgg tgatctccgc gatgcgctgt tccggattga ggatggtgat ctggctcgga
     8521 gcgtcgatgt caccggccgt cacgagcttc tcgcccgacg ccttgagcgc gatcgtgagc
     8581 ggcgtatcgc cgtgcatctg gagccggacg cgcttgaggt tgaggaggat cgtgatgacg
     8641 tcttccttca cgcccgcgag cgtcgagaac tcatggtcga cgccgtcgat cttgacctgc
     8701 gtgatcgcgg cacccggaag cgacgagagg atgatgcggc gaagcgagtt gccgagcgtg
     8761 tggccatagc cagggtagag cccgtctatc tcgaagaccc ccgagaactc ctcctccttg
     8821 atgacccgcg gcttcgacgg caatgtgacg ttggtttcaa gcatggtgtt tggattacgt
     8881 ataactaaaa cgattatcgg ctgtagtact cgagaacctg ctgtacgtcg aagatggtct
     8941 ccgcaggttc gtacgacggt tcggctttca tctcccccga gagcttcttc gcatcgagcg
     9001 tgagccacgc gggaacggtc gccgatgcga gcgtctcgcc gagcgtgacg aagagcggcg
     9061 actgcttgct ctgctcgcgg acggacacgg tgtcgccgat cttaacgcgg tacgacggga
     9121 tattgaaacg acggccgttc acctgaatgt ggccgtgcga tacgagctga cgcgcgaggc
     9181 gtcgcgtctt cgcgagaccg agtcggtata cgacgttgtc gagacgcgac tcgaggcgct
     9241 cgttgagacg gaggacgggc tgggtgcccg cggcgaccgc gtcgcgaacg tagttcgaga
     9301 gctgcttctc tgagataccg tagaggaagc gcatcttctg cttctcgatg agctgcttct
     9361 tgtagtcgga cgtcatgccg ccgcggcgca tcttcttgtt gcgcgcgctt cgcgcctccg
     9421 agagggcgaa tttctgcgtt tgcgtctttt cgaagaccgc tgctccgaga cgcttcgcta
     9481 ttttgtattt cggaccgatt ttcatatgag ttatttagat gcgtcgaggc ttagggagtc
     9541 gtgggccgtt gtgcgggacc ggtgtctggt ccgcgatctt cgtgatggcg atgcccttcg
     9601 acgagaagcc gcggagcgcg gactcgcgtc ccgcgccgat accgcggatg acgaccgagg
     9661 cgtccttcat acccatctgc gcggccttgt cgccgatcat ctcaccgacc ttcgctgcgg
     9721 cgaacggcgt cgacttgcgc gaacccttga atccgagcgc tcccgatgaa acggtgatga
     9781 gcgagttacc cttcgtgtcg cagagggtga gcatcgtgtt gttgaaggtc gcgagcacgt
     9841 tcaaaacgcc cgaagcgacc ttcttcttcg gcacacgcgt aagcgcggtt cccgttacgc
     9901 ttgagtctcc tcccttcttt atgattcgtt tcttacccat aggtcaatag aattgatagt
     9961 ggcgttaagc ggtctaggtc ttctcgagct tacggcgtcc cgagcccatc gtcttcttga
    10021 cgcctttgag cgttcgcgcg ttcgtcttcg tgcgctgtcc gcgcgcgggg agacgcttcg
    10081 cgtgacggct gccgcggtac gacttgatgt ccttgagacg cttgatgttc cccgtcacct
    10141 cgcgcttcag ctcaccctcg atggtgaacg actccacttt ctcgcggatg gcgttctcct
    10201 cgtcggtcgt gaggtccttc gcgctcttca tcgggtcgat gctaagcgac ttgagtatcg
    10261 aaactgcgcg agaatgcccg atgccgtaca ccgccgtgag gccgtactcg aggtgcttcg
    10321 tatcgggaat ggtgatgcct gagattctca tggttatcct tgacgctgct tattgcgggg
    10381 cttcttcttg ttaatgacgt agacgtagcc gcgccgcctg acgatcttgt cgtccgggct
    10441 gatcttcttc actgatgatc gaattttcat acgctgtgat taaagtcgct tcgtgatgcg
    10501 ggccttgccg ccgtacggtt ccgggacgag cgcgacacgg tccccgacga gcacccggat
    10561 cttgaatttg cgcatcttcc ccgcgaggta ggcgaggatg ggcttctcct ctccgtccaa
    10621 taaaactcga aaaagcgcat tagggagcgc ctcttctact gtcccgtaga cgagattgtc
    10681 tttgtctgat tgttcctgca tcaaacgtcg gagagtctaa caaagttgcg ccgatgcgtc
    10741 aaccccggat gctcccattt tagcgatatt ttaacctggc ccgtcacttc ttgtcggtga
    10801 tggtcgtcac cgcgatgtcg ttcgagtcgt cggggaaact gccctcccag aacggtctca
    10861 cctcggcctg tgcgttcgcg atcgccggat agctgccgaa aacggcggtc gcttctgtct
    10921 ttttcttacc cttgaggtcg ttcttgagct tattctcgtc gaacgtccag atgagccgcg
    10981 cgttgccctt gaggaggaac tcgagcgagt cgagggtcga aatgtcggag acggtcgtcg
    11041 tcgcggacgt gtacgtgaag gcgagcgact cgggattcgg aatggacacc gcgtcacccg
    11101 cgtagtctgg aacggattgt ttcgcgatga aggacgcgaa ctcgctctcc ttgaacatcg
    11161 gtacctggag tttcgccttc tccttgatgg tcgcgagcga atcgccgtac tcggtcgatg
    11221 ggagcgattc gaaggagacg gcgatggagt cgtcgtagat gatgaatccg gccggacgtt
    11281 cctccttaag acgcgcgaga agcgtgtcgc gaagttcgag ctggagcgct tgttccgccg
    11341 tctgaagttc cgccggatcg atgatgtatt tctcgccttc gaagccgccc gtgaacgcga
    11401 tggtcgattc cccatagacg ctatcgtact gatcggtgcc cttgaggccg gggacggtga
    11461 acttcgtggg tccgatgttg tacttctctc ccggctggtc ggcgaagacg gtcgcgacga
    11521 cggttccggg aacctcgttc cccttcgcgt ccttcgtcac gccgggaacc tcgatcgact
    11581 ccttgatgcg gtagatgaga ccgtccgggc tctcaaagcg cgtgttcttg atgagacgct
    11641 gcggcaacgt gctcttcgag ttgtagacga tgatctttcc ctcggcctgc gtcgcgacgt
    11701 tctcctttcc cgtcgccttg acctgcttct cgcctgtcgc ctcgagcgag aggagctcgt
    11761 acccgagctc gcccgcctgc ggcgtcttct tcgcggtgaa gtcggcctgc acgaagacgt
    11821 cgcgcgtctt cgggtagacg gtgacgtcgg cgcccgagag catgacgttc acggcgaagc
    11881 cgagcgcgag gacgacgacg gcgatgacgc tcgtcgtgat gatgcgcttc tttcgtgcgc
    11941 cgtttccgtc gacgacgtcc aggcgctcga tatcggagtc atcgaagcga ggcggttcct
    12001 ccggctcggc cggtcgcggc gctacgtcca tgcgcgcctt gcgagactcg gccggccgcg
    12061 gagcaacggg tttgcgcgcg ccggcgggcc tgatgtcctg caggttccac tttttagcca
    12121 tatgactagt atatactccc ctccttagac gcgcttctcc ccagcatcgt atttgtggaa
    12181 aaatctcgct tcgagcgcga ggtacgtatc agcgctcttt cgaccgccga cctcctcgac
    12241 gagccggcgt tcgatggcgg tgaccgtcgg cttcttcgcg atgaccgaac gaatgccgat
    12301 cgtcaccgcg tgcgtgaaga ggttcgtgaa ggggtactgc gccgcgagga tgacgtgcat
    12361 cgggaacgct cttttttcga cgatg
//
LOCUS       DASTSP010000071         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1274392, whole genome shotgun sequence.
ACCESSION   DASTSP010000071 DASTSP010000000
VERSION     DASTSP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274392"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..643)
                     /gene="clpP"
                     /locus_tag="VFS75_03280"
     CDS             complement(47..643)
                     /gene="clpP"
                     /locus_tag="VFS75_03280"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEU4677713.1"
                     /translation="MEKETIFNQLVPMVIEKTQGGERAFDIFSRLLKERIIFVTGPID
                     DHMANLIVAQLLFLEAEDPKKDIAMYVNSPGGSVSAGLAIVDTMNHVKPDVATVCVGL
                     AASMGSIILSQGKKGKRSILPNAEVMIHQPWGGTQGQASDIEITARHILKTRDLLNQM
                     LAKASGKPLSQIEKDTDRDFFMTADEAKKYGLVDVILK"
     gene            complement(723..1028)
                     /locus_tag="VFS75_03285"
     CDS             complement(723..1028)
                     /locus_tag="VFS75_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677714.1"
                     /translation="MKTLKVIRITHAFQSFAPADIRAAWIGCRIPLHEEQVKAIVRLR
                     ALDENGAHLVLKEDAIRALEAMGRNDAAHYWRNQPMEVIAFPKSCCELAEEQDLSRP"
     gene            complement(1146..1394)
                     /locus_tag="VFS75_03290"
     CDS             complement(1146..1394)
                     /locus_tag="VFS75_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677715.1"
                     /translation="MAPIPVSMAWKDLIVVTDGQALQNFSGPPVWRSLGETMFFVRFE
                     DGIAALIAAKKDDAVWYWRRNPVDHFRFPKTICTILPA"
     gene            complement(1514..2653)
                     /locus_tag="VFS75_03295"
     CDS             complement(1514..2653)
                     /locus_tag="VFS75_03295"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU4677716.1"
                     /translation="MEKEVLKSIALERRPGSSVRLFGELPYAELERHRSAAVAALGKE
                     VAIDGFRKGHVPENILVRHLGEMAVLTEMAERALGEAYPTLIEMHGLDVIGYPRIELT
                     KLAKDNPLGFAITVAVVPDIELPDYMKIAKDVNDGKESKDVTDEDVTTQINDILRQKI
                     AYERLQKKAAAHAHDHEHAGHDHDHAEHAHEHAEESAEKPEDIVLPELTDEYVKGLGQ
                     PGQFTSVDDFRTKIKEHLTIEKARDVDSRHRAKITDAIIEQTTMELPQVLIDAELNQM
                     FSQMEEDLKRAQLTMDDYLAHIKKTKEDLKTEWTPSAEKRAKLQLVLNAIAKKEAITA
                     DPELVESEVANLLLRYKDADKQRVRTYVESVLANEAVMKKLEEAK"
     gene            complement(2732..3112)
                     /locus_tag="VFS75_03300"
     CDS             complement(2732..3112)
                     /locus_tag="VFS75_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677717.1"
                     /translation="MTDNTTNNTETTADTARGNFEMKMPEGYDAAENHHTRLGIILAI
                     LIIVLVIIFGGLYLWGRMLGSEAVAPAPIVNDEPETPRAAADATILDTTSPSDDLDAI
                     NADLESTNLESIDADLTAIDGELQ"
     gene            complement(3123..3725)
                     /locus_tag="VFS75_03305"
     CDS             complement(3123..3725)
                     /locus_tag="VFS75_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677718.1"
                     /translation="MRTLDRSHKALYYALAVAAGVLLFGLEAAAAPDPAVTPESAVTA
                     PTPARGRTALSSAVQDRIVNLASNISLRLTAATGRYGDIIARLQTRMEKMRAAGADTA
                     AAEAKLAEAKASLTEAMTALDALGSVKDAISGETPRESFRAVRTNILAIRDDLKKTHA
                     LLRDTIALLKDALRASNAEKGVSDAVSNAPESTTAPATSE"
     gene            complement(3827..>4217)
                     /locus_tag="VFS75_03310"
     CDS             complement(3827..>4217)
                     /locus_tag="VFS75_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEU4677719.1"
                     /translation="TAQDYPFDYGFVPQTLFDDGDALDVVLLTTYPLMPGILVKARPV
                     AIMEMVDGGDRDDKIVAVPVDDPRFEEVRDLTDLNPHFVKEMTHFFETYKKIQNKEVK
                     TGAWHGVAEAKAAFTRSVSMYNEKKTA"
BASE COUNT          697 a         1252 c         1302 g          966 t
ORIGIN      
        1 cgcctccttg tggtcggcga aacatcgctc cggctcttgg tggacgtcac ttgagaatga
       61 cgtccaccaa gccatacttc ttcgcttcat ccgcggtcat gaagaagtcg cggtcggtat
      121 ccttttcgat ctgcgagagc ggtttcccgg acgccttcgc gagcatctga ttgagaaggt
      181 cacgcgtctt caagatgtga cgcgcggtta tctcgatgtc cgacgcctgg ccctgggtgc
      241 cgccccacgg ctggtggatc atcacctcgg cattggggag gatcgagcgc ttacccttct
      301 tgccctgcga gaggatgatg gagcccatcg acgcggcgag accgacgcag acggtcgcga
      361 cgtccggctt cacgtgattc atcgtgtcga cgatcgcgag acccgcggag accgaaccgc
      421 cgggggagtt cacgtacatc gcgatgtcct tcttcggatc ctccgcctcg aggaagagga
      481 gttgcgcgac gatgaggttc gccatgtggt cgtcgatcgg cccggtaacg aagatgatgc
      541 gctccttgag gaggcgcgag aagatgtcga aggcgcgttc gccgccctgg gtcttctcga
      601 tgaccatggg aacgagctgg ttgaatatcg tttctttttc catatgcgtc aaataatacc
      661 accaaattcg ttaatgggtt actccaagaa aatcccgccg aagcagggag tttcctcacg
      721 agtcatgggc gggaaagatc ttgttcttca gcgagctcgc aacagctttt cgggaacgcg
      781 atgacctcca ttggctggtt tcgccagtag tgtgcggcat cgttccttcc catcgcctcg
      841 agtgcccgga tagcgtcctc cttgaggacg agatgcgcac cgttctcatc gagggcgcgc
      901 aaccgcacga tcgccttaac ctgttcctcg tggaggggaa tgcgacagcc gatccaggcc
      961 gcccttatgt ccgccggcgc gaagctttgg aacgcatgcg tgatgcgaat caccttcaac
     1021 gtcttcacgt ctcctccgta tagtagtcgt acgtaccttc ttccgggaag ctacttagta
     1081 tcgaggatac gctcgaccgg cgaatagtca acttgcggga acgagaaaag aattgccccg
     1141 ccgtgtcagg cggggagaat ggtgcatatg gttttgggga aacgaaaatg gtcgaccggg
     1201 ttccgtctcc agtaccagac ggcatcgtct tttttcgccg cgatgagcgc cgctatcccg
     1261 tcttcgaatc gaacgaagaa catcgtctcg ccgagcgacc gccacacggg aggacccgaa
     1321 aagttctgaa gcgcctggcc gtcggtcacg acgatgaggt ctttccatgc catcgagacg
     1381 gggatcggcg ccatcccctc acgatgggcg tcgtttatac gtatacgcat acgcatctcc
     1441 tgtgagtatc cgtttcgaga gtacgtccaa gcgggcacga cgtcaagaaa tggccggcgg
     1501 tgccggccat ttcctatttc gcctcttcga gtttcttcat gacggcctcg ttcgcgagga
     1561 ccgattcgac gtacgtccgg acgcgctgct tgtcggcgtc tttgtaacgc agaaggaggt
     1621 tggcgacctc gctctcgacg agctcggggt cggcagtgat ggcctccttc ttcgcgatcg
     1681 cgttcaatac gagctggagc ttcgctcgtt tttcggcgct cggcgtccac tcggtcttca
     1741 ggtcctcctt cgtcttcttg atgtgcgcga ggtagtcgtc catggtgagc tgtgctcgct
     1801 ttagatcctc ctccatctgc gagaacatct gattgagctc cgcgtctatg agaacttgag
     1861 ggagttccat cgtcgtctgc tcgatgatgg cgtcggttat cttcgcacga tggcgagagt
     1921 cgacgtcgcg agccttctcg atggtgaggt gttcctttat cttcgtccgg aaatcgtcga
     1981 ccgaggtgaa ctgcccgggc tgtccgagac ccttcacgta ctcgtcggtg agttcgggga
     2041 ggacgatgtc ctctggcttc tccgcggact cctcggcatg ctcatgagcg tgttctgcat
     2101 ggtcgtggtc atgacccgcg tgctcgtggt catgcgcgtg cgccgccgcc ttcttctgga
     2161 ggcgttcgta cgcgatcttc tggcgaagaa tgtcgttgat ctgcgtcgtc acgtcctcat
     2221 cggtgacgtc cttcgactcc ttcccgtcgt tcacgtcctt cgcgatcttc atgtagtcgg
     2281 ggagttcgat atcgggaacg acggcgaccg ttatggcgaa tccgagggga ttgtccttcg
     2341 cgagcttcgt gagctctatc cgtgggtagc cgatgacgtc gagaccgtgc atttcgatga
     2401 gcgttgggta tgcttcgcca agcgcgcgct cggccatctc ggtgagaacg gccatctcgc
     2461 cgaggtgccg gacgaggatg ttctcgggga cgtgtccctt acgaaagccg tcgatcgcga
     2521 cctccttccc gagcgccgcg accgcggcgc tccgatgtcg ctcgagctcc gcgtacggga
     2581 gctcgccaaa gaggcgcacc gaggagccgg gccttcgctc gagcgctatc gatttcagta
     2641 cttctttttc cataccgcgt cagtgtatac aagttccgcg tatttgcaac aaaaaacggc
     2701 cgcggcatag ccgcggccgt tttcgttcgc actattgcag ttctccgtcg atcgccgtga
     2761 ggtccgcgtc tatcgactcg aggttcgtgc tctcgaggtc cgcgtttatc gcgtcgaggt
     2821 cgtccgaggg actcgtcgtg tcgaggatgg tcgcgtccgc cgcggcacgc ggcgtctcgg
     2881 gttcgtcgtt cacgattggc gccggcgcga ccgcctccga gccgagcatc ctgccccaga
     2941 gatagaggcc gccgaagatg atgacgagaa cgatgatgag aatcgcgagg atgataccga
     3001 ggcgggtgtg gtggttctcc gccgcgtcat acccttccgg catcttcatc tcgaagttgc
     3061 cgcgtgcggt gtccgcagtt gtctccgtgt tgttcgtagt gttatctgtc atggtgaaat
     3121 gattactcgc tcgtcgcggg tgccgtggtc gactccggcg cgttcgacac cgcgtcgctc
     3181 actccctttt ctgcgttcga tgcgcggagc gcgtccttga ggagcgcgat cgtgtcgcga
     3241 aggagcgcat gcgtcttctt gaggtcgtca cgtatcgcga ggatgttcgt acggacggcg
     3301 cggaacgatt cccggggcgt ctcgccgctt atcgcatcct ttacggaacc gagggcatcg
     3361 agcgccgtca tcgcctcggt gagcgatgcc ttcgcttcgg cgagtttcgc ctccgccgcc
     3421 gcagtgtccg cgcccgccgc gcgcatcttc tccatgcgcg tttgaagccg cgcgatgatg
     3481 tcgccgtagc gcccggtcgc cgccgtgagg cggagcgaga tgttcgacgc gaggttcacg
     3541 atacggtctt gtacggcact cgagagcgcg gtgcgtccgc gtgcgggcgt cggcgcggtt
     3601 accgcgctct ccggcgtgac cgccgggtca ggagccgctg ccgcttcgag tccgaagagg
     3661 agaacgcccg cggcgacggc gagcgcgtaa tagagtgcct tgtggcttcg gtcgagtgtt
     3721 ctcatggtgc gcgtgattat gggtatatac cttatatacg cctagtatag aacggccgga
     3781 ccctcgggtc cggccgttct gtgcacagaa acgcggttcg gtcgcgttac gccgtctttt
     3841 tctcgttgta catcgagacg ctccgcgtaa aggcggcctt cgcctcggca acgccgtgcc
     3901 acgcgcccgt cttcacctcc ttgttctgta tcttcttgta cgtctcgaag aagtgcgtca
     3961 tctccttcac gaagtgggga ttgaggtccg tgaggtcgcg cacttcctcg aagcgcgggt
     4021 cgtcgaccgg gaccgcgacg atcttgtcat cacggtcgcc gccatcgacc atctccatga
     4081 tggcgacggg gcgcgccttc acgaggatgc ccggcataag cggatacgtc gtgaggagga
     4141 cgacatcgag tgcgtccccg tcgtcgaaaa gggtctgcgg cacgaagccg tagtcgaacg
     4201 ggtagtcctg cgccgtg
//
LOCUS       DASTSP010000072         7291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_382215, whole genome shotgun sequence.
ACCESSION   DASTSP010000072 DASTSP010000000
VERSION     DASTSP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382215"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..998
                     /locus_tag="VFS75_03315"
     CDS             609..998
                     /locus_tag="VFS75_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEU4677720.1"
                     /translation="MRPRVSLLPVVPVIPAPTVAASAFSFLRNLGIGSTGADVSELQK
                     RLAAAGHFSGPVTGYFGPMTEAAVRAFQGTSGVPATGYVGQLTLGKLNGMGASVAAST
                     ADHSAEIVSLQQQLVGLLQELAVMLKK"
     gene            1085..2185
                     /gene="ychF"
                     /locus_tag="VFS75_03320"
     CDS             1085..2185
                     /gene="ychF"
                     /locus_tag="VFS75_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003344608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU4677721.1"
                     /translation="MSLSVGIVGLPNVGKSTLFNALTKKSVPAENYPFCTIDPSVGVV
                     AVPDNRLDTLSKLSRSEKTIPAIVEFVDIAGLVKGASSGEGLGNKFLANIREVDMIAE
                     VVRIFDDPDVVHVSGAVDPLSDIEVINLELILADAETVGKRLSNVEKDAKRGDKGALI
                     EKGALEKLSQHLASGRLANQCAFDELELPFVAQLQLLTMKAILYVFNKKHGAYNLDET
                     NDERWSELMGFIEETGSEYVVIDAGVEHELKDLDEADKVEFRREYATLDSGIDTLIRA
                     AYHRLGLMSYFTTGEKETRAWTVKRGSTGPEAGAAIHTDFKTKYIRAQVVTFEDLVSS
                     GSFAAAREKGKLRTEGKEYIVKDGDVIEFMIA"
     gene            2264..2488
                     /locus_tag="VFS75_03325"
     CDS             2264..2488
                     /locus_tag="VFS75_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677722.1"
                     /translation="MKTIAAVANNVLEHTKYILLAALLILVSFTIGTFLLRLFDVHAP
                     LSLDVKHGVIPFFGAATASMLMTKRFSKKD"
     gene            2490..2765
                     /locus_tag="VFS75_03330"
     CDS             2490..2765
                     /locus_tag="VFS75_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU4677723.1"
                     /translation="MRSWYVYMLRCSDGSLYTGVTTDVERRLAEHKAGKVGAKYTRSR
                     IPLHIAYTETCASRSEAQSREFALKRLSKAEKESLAKRAPKKRRVRR"
     gene            2835..4634
                     /gene="typA"
                     /locus_tag="VFS75_03335"
     CDS             2835..4634
                     /gene="typA"
                     /locus_tag="VFS75_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU4677724.1"
                     /translation="MNPQETRNIAIIAHVDHGKTTLTDAIMRQTGAVSDENATMDSNS
                     LEKERGITIYAKNASIEYKGTKINIVDTPGHADFGSEVERVLRSIDSVLLVVDAQEGP
                     MPQTRFVLKKSLELGIKPIVVINKIDKPAADPHKAEELVLELFLELGANDDQANFTTV
                     YAIGRQGIAKRKLDDVSSDLTPLLDTILEKVPPAKGYVEGEALRAQVFNLGYDNFLGR
                     LAVARIYDGAMKDGQNLFLLHDGDAPKKGKVTKLFTFKGTERIESKIAYAGDIVIVAG
                     FTDVNIGKTFADVDASLPLPAIAVDQPTIALDFLVNNSPFAGREGKFVTSRQIRERLE
                     RELEVNVGLKVDFGTGDIFKVYGRGEMHVSILLENMRREGYELQVSQPQAIIEERPDG
                     KYEPFEEVTVDIPSEYQGAVIEKLGRRAFIMKDMSVHENQVRLILEGPTRGLLGYRGQ
                     FVIDTRGEGILCSRVLGFRPYAGEIKKRQSGSMISMAPGKALGFALWNLQDRGELYIG
                     PGTEVYEGMVIGNTSKGEEMEVNPTKGKQLTNMRASGSDESIYLTPPRELSIEVGLEI
                     MADDEYLEVTPSSTRLRKQVLKESERVRARRQG"
     gene            4668..5060
                     /locus_tag="VFS75_03340"
     CDS             4668..5060
                     /locus_tag="VFS75_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677725.1"
                     /translation="MKPAYIYAALVVLVVGGLVALKQFGGTSNAADMAPYDDFAQCLT
                     DHGVKFFGAYWCPHCQAQKRLFHNSKKLPYIECSTPDGQSQTQICIDNKITGYPTWEF
                     ADGSRLDGEQTFEDLGAKADCPVPQAAT"
     gene            5057..5596
                     /locus_tag="VFS75_03345"
     CDS             5057..5596
                     /locus_tag="VFS75_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEU4677726.1"
                     /translation="MIRVETIETLNGILAYAGVAALVVTALLIIDMRGKRALAPYIVK
                     WGLLATFVVALAGTVLSLVYSDVFGLVPCGLCWFERIMLYPQVLLVGTALIVRERTIP
                     RYGIVLSTVGLVISLYHHYIQMGGGEFVRCPAAGVGADCAKRFMFELGFITFPLLGAI
                     SFALLIALYVYLRREYARS"
     gene            complement(5593..5862)
                     /locus_tag="VFS75_03350"
     CDS             complement(5593..5862)
                     /locus_tag="VFS75_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677727.1"
                     /translation="MNPEELQNSPKLFCENIRIGYSPEYFVMALASGAQATIYSLTPE
                     HAKRLYEYLGHELAAFEKRHGTIDATWNPNVVSPVQPFHEPGEGS"
     gene            6040..6954
                     /locus_tag="VFS75_03355"
     CDS             6040..6954
                     /locus_tag="VFS75_03355"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEU4677728.1"
                     /translation="MENTAKNFALQLGALITMYVSVVALIALLLSIITLLYPDVAQAP
                     YERTAAASTVRYTIATLIVFFPAYVVLTRVVNTMRRAEAGLYLGLTKWLIYLSLLIGG
                     GVLLGDVVGVVNSFLNGELTIRFILKALAVLIVVGAAFTYYLADVRGYWRTREASSLR
                     YGAAVSILVVLAIVYGFTKIEAPSEVREMNIDRQEVADLQRIEMQVENYHQVNDALPE
                     SLTDAFGSLPVPSASAGRGAYAYELMTEDTYRLCADFTYPTSDAERSPYEVVNPETGV
                     TKTSYSWDHGAGRWCFERSVAVRPAPVK"
     gene            7024..>7291
                     /locus_tag="VFS75_03360"
     CDS             7024..>7291
                     /locus_tag="VFS75_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4677729.1"
                     /translation="MHRKYDHKEIDAKWQKAWDESGIYDVGARDPKRDKEYVLVEWPY
                     PSGNLHIGHWFAFAVVDIYVRARRMQGKQVLFPIGFDAFGLPAEN"
BASE COUNT         1494 a         2239 c         2126 g         1432 t
ORIGIN      
        1 ccgtcaatgg cgtgagcact cacaatgaag ttggacctga tatccgcatt gatgacgtaa
       61 ccaaagcagc aagtgtcacc gataccgatg agcagtattc ttcagtcacg actggcacta
      121 cacaagatta ttatcaaaat aatggcgcag tagtagacgt cgtaattccc gccggtaccg
      181 tatttagtgg cagtgcgtca tgggacggta tagtgaatcc gccaaccgtg acgacagcgg
      241 ctctggatat tccgggattt gacaccaccg ttacctcagc aattaccatt ggatcaagcc
      301 ttagcgacct taccttcgac catgcagtgc gtctcaggtt tgcaggccaa gcgggcaaac
      361 atgtcggttg gtataacagc gccggaatat tcactgagat aactgctgat tgtattgcaa
      421 cgggtggcga tgatgagact gcccaaacaa cacaactccc ggcagcctca agctgttcga
      481 tggatgtggg aagtgatctc gccgtttgga ccaaacactt ctctacattc gttacatata
      541 cccagacggc tcaaacccct gtaggcagtc acggtaagaa gcagggaacg agggtaccga
      601 gctcaaactt gcgtccgcgg gtctcgctcc ttccggtcgt tccggtcata ccggcaccga
      661 ccgtcgccgc atcggcgttc tcgttcctcc ggaaccttgg catcggctcg accggcgctg
      721 acgtctcgga gcttcaaaag cgactcgccg ctgcgggaca cttcagcggt ccggtgacgg
      781 gttacttcgg tccgatgacc gaggcggcgg tccgcgcctt ccagggtacg tcgggcgttc
      841 ccgcgacggg atacgtcggg cagctcaccc ttgggaagtt aaacggcatg ggtgcgtcgg
      901 tcgcggcttc aaccgccgac cattcggccg agatcgtgtc gctccaacag caactcgtcg
      961 ggctcctcca ggaactcgcg gtgatgctca agaagtaatc gttataaagt agaaaacgcg
     1021 ccggagacct ccggcgcgtt ttcgtgcttt aaaagtacga ggaaatctgg tacgctggcc
     1081 gtctatgtcg ctctctgtag ggatcgtggg acttccgaac gtcggtaaat cgacgctctt
     1141 caacgcgctc acgaagaagt cggtgccggc ggagaactat ccgttctgca ccatcgaccc
     1201 atcggtcggc gtcgtcgccg tccccgacaa tcgcctcgac acgctctcga agctctcacg
     1261 ctcggaaaag acgattcccg ccatcgtcga gttcgtcgac atcgcgggcc tcgtgaaggg
     1321 cgcgtcctcg ggcgaggggc tcggcaacaa gttcctcgcg aacattcgcg aggtcgatat
     1381 gatagccgag gtcgtgcgca tcttcgatga cccggacgtc gtccacgtct cgggcgccgt
     1441 cgacccgctc tccgatatcg aggtcatcaa tctcgagctc atcctcgcgg acgcggagac
     1501 ggtcgggaag cgtctttcga acgtcgagaa ggacgcgaaa cgcggcgaca agggagcgct
     1561 cattgagaaa ggagcgctcg agaagctctc ccagcacctc gcgtccggaa ggctcgcgaa
     1621 ccaatgcgcg ttcgacgagc tcgaactccc gttcgtcgct caactccaat tgctcaccat
     1681 gaaggcgata ctctacgtct tcaataagaa gcacggcgcg tacaatctcg acgaaacgaa
     1741 cgacgagcgt tggtcggaac tcatgggctt catcgaggaa acgggctcgg agtacgtcgt
     1801 catcgacgcc ggcgtcgagc acgagctcaa ggacctcgac gaggccgata aggtcgagtt
     1861 tcgtcgcgag tacgcgaccc tcgattcagg catcgatacg ttgatccgcg cggcgtacca
     1921 tcgcctcggg ctcatgtcgt acttcacgac gggcgagaag gaaacgcgcg catggacggt
     1981 gaagcggggt tcgaccggcc cggaggccgg cgcggcgata cacacggatt tcaagacgaa
     2041 atacatacgg gcgcaggtcg tcacgttcga ggaccttgtc tcgtccggtt cgttcgcggc
     2101 ggctcgcgag aaggggaagc ttcggacgga gggaaaggaa tacatcgtga aggatggcga
     2161 cgtcatcgag ttcatgatcg cgtagagatg acgaatcgat gaaaatcgcc attgtagcgg
     2221 gatgaattcg ctcgtatact gtgtgcctaa tccaaagtca catatgaaaa ccatcgccgc
     2281 agtcgcgaac aacgtcctcg agcacacgaa atacattctc ctcgccgcac tcctcattct
     2341 cgtctcgttc accatcggaa cgttcctcct tcgattgttc gacgtccacg cgccgctgtc
     2401 gctcgacgtg aagcacggcg tcattccgtt cttcggcgcg gcgaccgcgt cgatgctcat
     2461 gacgaagcga ttctcgaaga aggactgaca tgcgctcctg gtacgtctac atgctccgct
     2521 gtagcgacgg ctcgctctac acgggggtga cgacggacgt cgagagacga ctcgccgaac
     2581 ataaggcggg caaggtgggc gcgaagtaca cgcgttcgcg cataccgctc cacatcgcgt
     2641 acacggagac gtgtgcgtcg cggagcgagg cgcagtcgcg cgagttcgcg ctgaagcggc
     2701 tctcgaaggc cgagaaggaa tcgctcgcga aacgcgcgcc caagaagagg cgcgtccgcc
     2761 gctagtgctt tgacgaacgg gccattatct agtactcttt tcgcacatcg cccattcggg
     2821 gcatttttcg tttcatgaac ccacaggaga cacgcaacat cgccatcatc gcgcacgtcg
     2881 accacggcaa gacgacgctc accgacgcca tcatgcggca gaccggtgcc gtgagcgacg
     2941 agaacgcgac catggactcg aactcgctcg aaaaggagcg aggcatcacc atctacgcga
     3001 agaacgcgtc gatcgagtat aaaggaacga agatcaacat cgtcgacacg cccggacacg
     3061 cggacttcgg ctcggaggtc gagcgcgttc ttcgctcaat cgactcggtg ctcctcgtcg
     3121 tcgacgcgca ggagggaccg atgccgcaga cgcgcttcgt cctcaagaag tccctcgaac
     3181 tcggcatcaa acccatcgtc gtcatcaaca agatcgataa gccggccgcg gaccctcaca
     3241 aggccgagga gctcgttctc gaactgttcc tcgagctcgg cgcgaacgac gaccaggcga
     3301 acttcacgac ggtctacgcg atcggcaggc agggcatcgc gaagcgaaag ctcgacgatg
     3361 tgtcgtctga cctcacgcca ctcctcgata ccatcctcga gaaggtgccg cccgcaaagg
     3421 ggtacgtaga gggagaggcc cttcgcgcgc aggtcttcaa cctcggctac gacaacttcc
     3481 tcggtcgtct cgccgtggcg cgcatctacg acggcgcgat gaaggacggc cagaaccttt
     3541 tcctcctcca cgacggcgat gcgccgaaga aggggaaggt cacgaagctt ttcacgttca
     3601 agggaaccga gcgcatcgag tcgaagatcg cgtacgccgg tgacatcgtc atcgtcgcgg
     3661 ggttcaccga cgtcaatatc ggcaagacgt tcgcggacgt cgatgcctct ctgccgcttc
     3721 cggctatcgc ggtcgaccag ccgaccatcg cgctcgactt cctcgtcaac aactcgccgt
     3781 tcgccggacg cgagggcaag ttcgtgacgt ctcggcagat acgagagcgg ctcgagcgcg
     3841 aactcgaggt gaacgtcggc ctcaaggtcg atttcggtac gggtgacatc ttcaaagtgt
     3901 acggccgtgg cgagatgcac gtatccatcc ttctcgagaa catgcgccgc gagggctatg
     3961 agctccaggt ctcgcagccg caggcgatca tcgaggagcg tcccgacggt aagtacgagc
     4021 cgttcgagga ggtgaccgtc gacattccgt cggagtacca gggcgccgtc atagagaagc
     4081 tcggccgacg cgccttcatc atgaaggaca tgagcgtcca cgagaatcag gtgcgcctta
     4141 tcctcgaagg accgacgcgc ggactcctcg gctaccgcgg tcagttcgtc atcgacacgc
     4201 gcggcgaggg cattctctgc tctcgtgtcc tcggcttccg gccgtacgcg ggagagataa
     4261 agaagcgcca gtcgggctcg atgatatcga tggcgcccgg caaggcgctc gggttcgcgc
     4321 tctggaacct tcaggaccgc ggcgaactct acatcggtcc gggtactgag gtgtacgagg
     4381 gtatggtcat cggcaatacg tcaaagggag aggagatgga ggtcaatccg acgaagggaa
     4441 aacagctcac gaacatgcgc gcctcgggct cggacgaatc catctatctc acgccgcctc
     4501 gcgaactctc catcgaggtc ggccttgaga tcatggctga cgacgaatac ctcgaggtga
     4561 cgccgtcctc gacgcgcctc aggaagcagg tcctcaagga gtcggagcgc gtccgcgcac
     4621 gccgccaagg ctagaacggc aggccgaacg gtggtacact tctcggcatg aaaccggcat
     4681 acatctacgc cgcgctcgtc gtactcgtcg tcggcggcct cgtggcgctc aagcagttcg
     4741 ggggcacgtc gaacgcggcg gacatggcgc cgtacgacga cttcgcgcag tgtctcaccg
     4801 accacggcgt gaaattcttc ggcgcgtact ggtgtccgca ctgccaggcg cagaagcgtc
     4861 ttttccataa ttcgaagaag cttccgtaca ttgagtgctc gacgcccgat ggacagagcc
     4921 aaacgcagat ctgcatcgac aacaagatca ccggctatcc gacgtgggag ttcgccgacg
     4981 gctcacgcct cgacggtgag cagacgttcg aagacctcgg tgcgaaggcc gattgccccg
     5041 tgccccaagc cgcaacatga tacgcgtcga gaccatcgag acccttaacg gcatcctcgc
     5101 gtacgccggc gtcgcggcgc tcgtcgtaac cgcgcttctc atcatcgata tgcgcgggaa
     5161 gcgagcgctc gcaccgtata tcgtcaaatg gggtcttctc gcgacgttcg tcgtcgcgct
     5221 cgcgggtacg gtgctctccc ttgtctactc cgacgtcttc ggactcgtcc cctgcgggct
     5281 ctgttggttc gaacgcatta tgctctaccc gcaggtgctc ctcgtcggta ccgcgctcat
     5341 agttcgagag cgaacgatac cccgctacgg catagtgctc tctacggtcg gcctcgtcat
     5401 ctcgctctac catcactaca tccagatggg tggcggcgag ttcgttcgtt gtcccgcggc
     5461 aggcgtcggt gcggactgtg cgaagcgctt catgttcgaa ctcggcttca tcacgtttcc
     5521 gctcctcggg gcgatctcgt tcgcgcttct catcgcgctt tacgtatatc tccggcgcga
     5581 atacgcccgt tcctaactgc cttctccagg ttcgtggaag ggctggacgg gactcacgac
     5641 gttcgggttc caggtcgcat cgatagtgcc atggcgcttt tcgaacgccg cgagctcgtg
     5701 tcccagatac tcatagagcc gcttcgcgtg ctccggtgtg agcgagtaga tggtcgcctg
     5761 cgcaccggac gcgagcgcca tgacgaagta ttccgggcta tacccgattc gtatgttctc
     5821 gcaaaagagc ttcggcgagt tctgtagttc ttctgggttc atggcgtgtc gtgttacctc
     5881 gtaatccgtc cagtatagca aaatcgggtt tcggctttac aattcgccaa aatacctcac
     5941 aattcctaca tgacgctcct tatcggagtc attatcatcg ttttaataat cggcatcggg
     6001 acggcttcgc tcatggccgt tcgccgacaa cacgaaacca tggaaaacac cgccaagaac
     6061 ttcgcgctcc aactcggggc gctcatcacg atgtacgtgt cggtcgtcgc gctcatcgcg
     6121 ctcctcctta gcatcattac gctcctctat cccgacgtcg cacaggcgcc ctacgagcgt
     6181 accgcggcag cttcgaccgt ccgctacacg atagcgaccc tcatcgtgtt cttcccggcg
     6241 tacgtcgttc tcacgcgcgt cgtgaacacg atgcgacggg ccgaggcggg actgtatctc
     6301 ggactcacga agtggctcat ctacctttcg cttctcattg gtggaggcgt gctccttgga
     6361 gacgtcgtcg gcgtcgtgaa tagtttcctc aatggcgagc tcactatccg tttcatactg
     6421 aaggcgctcg ccgtcctcat cgtggtcggt gccgcgttta cgtactacct cgcggacgta
     6481 cgcgggtact ggcggacgcg tgaggcttca tcgctccgct acggtgcggc cgtctccatc
     6541 ctcgtcgtcc tcgcgatcgt ctatggcttc acgaagatcg aggcgccgag cgaagtgcgt
     6601 gagatgaaca tcgaccgtca ggaggtagcc gatctccagc gcatcgagat gcaagtcgag
     6661 aactaccacc aggtgaacga cgcgctcccc gagagcctca cagacgcgtt cggatcccta
     6721 ccggtgcctt cggcgtccgc aggacgcggt gcgtacgcgt acgagctcat gaccgaggac
     6781 acctaccgac tctgtgccga cttcacgtat ccgacgagcg acgccgagcg ctctccgtac
     6841 gaggtcgtga accccgagac cggcgtcacg aagacgtcat actcatggga ccatggcgcc
     6901 ggccggtggt gcttcgaacg ttcggtcgcc gtacgtcccg ctccggtgaa gtagcgcctc
     6961 tcgacattcc gttttttcgg ggcataatac gcaatacgtt tacacgcttc tttcccgtta
     7021 cccatgcata gaaaatacga ccacaaggag atcgatgcga agtggcagaa ggcatgggat
     7081 gagagcggta tctacgacgt cggggcgcgc gacccgaagc gcgacaagga gtacgtactc
     7141 gtcgagtggc catatccctc ggggaacctc cacatcggtc actggttcgc attcgcggtc
     7201 gtcgacatat acgttcgcgc gcggcgcatg caggggaaac aggtcctctt cccgataggc
     7261 ttcgatgcat tcggcctccc agccgagaac g
//
LOCUS       DASTSP010000073         7392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1501461, whole genome shotgun sequence.
ACCESSION   DASTSP010000073 DASTSP010000000
VERSION     DASTSP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501461"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..78)
                     /locus_tag="VFS75_03365"
     tRNA            complement(6..78)
                     /locus_tag="VFS75_03365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Val,seq:gac)
     gene            191..322
                     /locus_tag="VFS75_03370"
     CDS             191..322
                     /locus_tag="VFS75_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677730.1"
                     /translation="MAHTIVQIGTVVGIIGITLLASVLIVGCCLALPYLGERLAKTV"
     gene            complement(366..608)
                     /locus_tag="VFS75_03375"
     CDS             complement(366..608)
                     /locus_tag="VFS75_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677731.1"
                     /translation="MFDISWQDAVLTVGNCVFFIALLPSIFGPNKPSKWTSLSTAATL
                     TAFTATYLTLSLAFAPIATGATALAWWVLYFQKRKG"
     gene            665..739
                     /locus_tag="VFS75_03380"
     tRNA            665..739
                     /locus_tag="VFS75_03380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Arg,seq:ccg)
     gene            complement(899..985)
                     /locus_tag="VFS75_03385"
     tRNA            complement(899..985)
                     /locus_tag="VFS75_03385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Ser,seq:cga)
     gene            1102..1173
                     /locus_tag="VFS75_03390"
     tRNA            1102..1173
                     /locus_tag="VFS75_03390"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Glu,seq:ttc)
     gene            complement(1429..2142)
                     /locus_tag="VFS75_03395"
     CDS             complement(1429..2142)
                     /locus_tag="VFS75_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4677732.1"
                     /translation="MKTTLTGKKEVAEGTMLFSFAKPEGFEYMAGQSLDLILVNPPET
                     DDEGNKRAFSITSDPSSPELKIATRMRDTAFKRVLRDMGEGTEIEVEGPFGSFTLHEN
                     AARPAVFLAGGIGITPFHAMVHDATTRKLPNKIILFYSNRRPEDATFLEGLTRQASEN
                     PNFTFVPTMTDMEKSKAEWSGATGYIDEALVKKHVDLAASPIFYLAGPAGMVAAMRKM
                     LNDMGVSNDDIRTEEFSGY"
     gene            2292..2591
                     /locus_tag="VFS75_03400"
     CDS             2292..2591
                     /locus_tag="VFS75_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677733.1"
                     /translation="MFESFKARREVLPSVEFANWKSADTELEALSAAVTSKMEWEEED
                     GGDNIQFTPQEGYDESLKNLQKYLDGKGLRYSTEALATALTETLGKGPEYIETVS"
     gene            2655..2978
                     /locus_tag="VFS75_03405"
     CDS             2655..2978
                     /locus_tag="VFS75_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677734.1"
                     /translation="MFVSVRAAVAGVLEKTVPSSDRPHDVHYVTPRFAEWVQSGGRNP
                     AILEGNDAVHTAAASSVISRYLIEASALAERLLDQGVPEEVALGLLPYEVYVRLGLDK
                     YDKKL"
     gene            complement(3280..3918)
                     /locus_tag="VFS75_03410"
     CDS             complement(3280..3918)
                     /locus_tag="VFS75_03410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4677735.1"
                     /translation="MSTSRRSRPSSEIRATKRYYDSHANEWTARKTNSFHHEAEFTRL
                     ASLLRNGSEVLDIGCAGGILVPLFLGIGRHLRYTGLDISESFLKIARRRYPRLRFLRG
                     NIADAKTLPKKKFDGFVAAAVLMHVPRDLWDETFHNIERLMRPGAVGYLTLPVEHPSG
                     DRKHTDPRHFTLVSETEQRAYLRDRGWTILKRSKLKGFIGDELWRGYIVKLS"
     gene            complement(3896..4819)
                     /locus_tag="VFS75_03415"
     CDS             complement(3896..4819)
                     /locus_tag="VFS75_03415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4677736.1"
                     /translation="MHDLIIIGGGPGGVAAGVYASRKKLATVFITKEWGGQSTVSTDI
                     QNWIGTKNISGSDLAKNLEGHLRAYADDVLTVVSGRTAVKVEKLADAHFKVTLDNGDS
                     YEAKTLLITTGSTRRKLEVPGAEAYDQKGLTYCASCDGPLFAGQDVAVIGGGNAGFES
                     AAQLLAYAKSVTLLHRSENFRADPVTVEKVLAHPNMKAVKNVKIKEVVGDRFVTGVVY
                     DDAEGTTQTLPVAGIFVEIGLVPTTECVAGLVELDEVGRIKIDPWTGATSTSGVWAAG
                     DCTNIKYHQNNISAGNAVTALEDIYVHLKTK"
     gene            complement(5044..6270)
                     /gene="ftsZ"
                     /locus_tag="VFS75_03420"
     CDS             complement(5044..6270)
                     /gene="ftsZ"
                     /locus_tag="VFS75_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU4677737.1"
                     /translation="MEKIETDVESFARIRVVGVGGSGNNAVNYMVGAKVKGVEFIAIN
                     SDAQDLHHSLAKKKINIGKNLTRGLGAGGNPDMGRRAAEETREEIANAIKGSDMIFIT
                     GGMGGGTGTGAAPVVAKIARETGALTVGVVTKPFLFEGQERMRLALQGIEELKKEVDA
                     LITIPNDRLLAIVDRETSVMAAFQLCDNVLKQAVEGISDLITMPGIINVDFADIRSVM
                     ENAGSALMGVGVSAGEKRAEEAARGAINSPLLEVSITGAKGVLFAIAGGDDLGMLEIQ
                     DAARVITESIDPHAKVIFGAIKDDKLKKNEVRVTVIATGFPEMELHGVPSSMRIQERQ
                     TVPATEEKDLRGRIFNSIGGGKPEPVKDEPVVAREVREERAPVQKEPKPVDMIDEEPD
                     DDWGAVPAFLRRSKLK"
     gene            complement(6410..>7392)
                     /locus_tag="VFS75_03425"
     CDS             complement(6410..>7392)
                     /locus_tag="VFS75_03425"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEU4677738.1"
                     /translation="AVAQAEKASGVKVKRAYISMGGIGVDEIHSRGEAITSRADSVIT
                     QVDIDKAIQASEESIQDRIPNRKIIHAIPLSYTVDGEKVHGRPHDLKGTKLEVDVLFI
                     TAFEQHLNDLVGAIESIGIKVDDVMASPIAGSLVMLTKAQKRAGCVLANIGAETVSIV
                     VFENSIPVSLKVFPIGANDITNDIALGLKVTLDEAEKIKRGGMSQTPYSKKKLDEIVS
                     ARLTDIFELIDAHLRKIKRDGLLPAGIILTGGGSSIAMIQDLARVSLRLPSKIALLDP
                     GQNGKVRDATWAVGYGLCIWGITGTEEESGINLARTTGNTLLNWFKQFLP"
BASE COUNT         1393 a         2108 c         2219 g         1672 t
ORIGIN      
        1 atcggtgcgc ggtgcaggac ttgaacctac gacccccaca atgtcaatgt gatgctctac
       61 cagctgagct aaccgcgcgg tggtgccaat ataccagagc gtcgtacgat agggaagtcc
      121 aggctatagt attgatttct ctgaatgtgc cgtggtatgg tgcggacagt acaacagcga
      181 aggaaatgcc atggcgcaca ccatagtgca gatcggcacc gtagtcggca tcatcggtat
      241 tacgctcctc gcttccgtcc ttatcgtcgg ctgctgtctc gcgctcccgt acctcggtga
      301 gcggctcgcg aagacggtat gaagggaggc cctcgtgcgc aatgcgcacg agggccggtt
      361 ttatgctagc cctttctctt ttggaaataa agcacccacc acgcgagcgc ggtcgcgccg
      421 gtcgcgatgg gcgcgaacgc aagcgaaagc gtaaggtagg tcgcggtgaa cgccgtgagc
      481 gtagccgcgg tcgagagact cgtccacttc gatggtttgt tcggtccgaa tatggagggg
      541 aggagcgcga tgaagaagac gcagttgccg acggtgagga ccgcgtcttg ccatgagata
      601 tcgaacattg tcgctattgt accgtcattg cccaacgcat gtaaattcgg tactctactg
      661 ccacgcctcc atagtttagt ggatagaacg cgggattccg gatcccgtaa ccgaggttcg
      721 actcctcgtg ggggcaccat ataaaagacc gacggtcgtc ggtcttttat atggtgccgg
      781 gcggagcgat gcgagccgct tcaaaagcgg cgaggcagcg agccggggtc gagagtgctt
      841 agcgaattgg agtgtaacga acaattcgct tagcaactcg tgaccacata caagggtgcg
      901 gaagaggagg gattcgaacc ctcgatgggg tttcccccat gcatgatttc gagtcatgtg
      961 ccttcaacca ctcagccact cttccgtgtt attggtggtg ctcacgaaat gagcccctat
     1021 aacctagcat tttttgccga ttttcggtag tattgcggac aattgagtcg ggatatgtga
     1081 gcgtgtcccg ggtcgattcc cggccccatc gtctaacggt taggacagcg ggttttcagt
     1141 ccgtaaatcg gggttcgatt ccccgtgggg tcatgacatc agaaaagagc gcacgtgagt
     1201 gcgccttttc tgtccatgac cccaagcagg caaccttgag gttgcctgcg gcgaggaatc
     1261 gaatgacgga gcgatgttta cgagcgagtc gcgcccggtg ttcttttgaa cctagtgaat
     1321 tagaaaagac gggacgattc ccctgaaaca gtccgccaga ctgtttcagc cccgtggggt
     1381 cacaaacgaa aaagtccggc gcttgccgga ctttttcttc gaggaattct agtaccccga
     1441 aaactcttcg gtgcggatgt cgtcgttcga gacacccatg tcattgagca tcttccgcat
     1501 cgcggcgacc atgccggccg ggcctgcgag gtagaagatg ggggaagccg caaggtcgac
     1561 atgcttcttc acgagcgcct cgtcgatata accggtcgcc ccgctccatt ccgccttcga
     1621 cttctccata tcggtcatcg tcgggacgaa ggtgaagttc gggttctcgc tcgcttgccg
     1681 ggttaggcct tcgaggaacg tcgcgtcctc tggtcgacgg ttcgagtaga agaggatgat
     1741 cttattgggg agcttccgcg tcgtcgcatc gtggaccatc gcatggaacg gcgtgatgcc
     1801 gataccgcct gcgaggaata cggcgggtcg tgccgcgttc tcatggagtg tgaacgagcc
     1861 gaacggtccc tcgacctcta tctccgtacc ctcgcccata tcgcgaagga cacgcttgaa
     1921 cgcggtgtcg cgcatgcgcg tcgctatctt aagctccggc gaagatgggt cgctcgtaat
     1981 ggaaaacgcg cgcttgttgc cctcgtcgtc ggtctccggc gggttcacga ggatgaggtc
     2041 gagggattgg cccgccatgt actcgaatcc ttccggcttc gcgaacgaga agagcatcgt
     2101 tccctcggcg acctccttct tgcctgtcag tgtggtcttc atatgattga tggtgtgttg
     2161 ttctggtaaa tcgtgcaagt ctttctatta aaatgcatcg gacggggttg cgtcaaagca
     2221 ttactctcgg agagggcaaa gctatgaaca gagccttctg gacctcggtc ggggcctgtt
     2281 atcatgagag tatgttcgaa tcattcaaag cgagaaggga agttctgcct tccgtcgaat
     2341 tcgcgaattg gaagtcggcg gacacggagc tcgaagcgct aagcgcggca gtgacatcga
     2401 aaatggagtg ggaggaagag gatggcggcg ataacatcca gttcacgcca caagagggct
     2461 acgatgagtc gctcaagaat ctccaaaagt acctcgacgg taagggtctc cgctatagta
     2521 ccgaggcact cgcgacagcg ctcacggaga cgctcggtaa aggccccgag tacattgaga
     2581 ccgtatccta gttgtgctcc tcgaagaaaa gcgtatagtg gaggccgttt agacaaggag
     2641 gccatctcat gcctatgttc gtttcagtgc gcgccgcggt cgcgggtgtg ctcgagaaga
     2701 ccgtgccgtc ttcggatcga ccccacgacg tgcactacgt cacgccgcga ttcgcggaat
     2761 gggttcagag cggcggaagg aatcccgcta tcctcgaggg gaacgatgcc gtccatacgg
     2821 cggcggcgag ttccgttatc tctcgctact tgatcgaggc gagcgcgctc gccgagcggc
     2881 ttctcgacca gggagtacca gaagaggtcg ctctcgggct cttgccgtac gaggtttacg
     2941 tgcggcttgg cctcgacaaa tacgataaga agctgtgaca cgaaggaccc tgcccggggt
     3001 ccttcctttg tatttcgaac tggttttcca acatgctttc ccccagcgat atgaacggcg
     3061 ttgtacacag tcttatcaac aggatggccg atttcctact tctttgaggc cgttgtgcgg
     3121 ctacttgacg gcaggaactg ggagagggaa actatacatg tctttcgagt gtggtgaatt
     3181 tcttcacata catcgtaccc gtcatagggt gctaaggctc gggggacaag ctctacgttg
     3241 taagaaaaac cgctgagccg tcagcggttt ttctgtggcc tacgacagct tcacgatata
     3301 tccgcgccag agttcgtcgc cgatgaaccc cttcagtttc gaacgcttga gtatcgtcca
     3361 tccacggtcg cgtaggtacg ctcgctgttc cgtttccgac acgagcgtga agtggcgtgg
     3421 gtcagtgtgc tttcggtccc cactcgggtg ttcgaccgga agtgtgagat atccgaccgc
     3481 acccggtcgc atgagtcttt cgatattgtg gaacgtctcg tcccataagt cgcgcggcac
     3541 gtgcatgagg accgcagcgg cgacgaaacc gtcgaacttc tttttaggca aggttttcgc
     3601 gtccgcgata ttccctcgga ggaatcgaag tcgcgggtat cgtcgcctcg ctattttgag
     3661 aaaactttcg gagatatcga gccccgtgta tcgaaggtgt ctcccgatac cgagaaagag
     3721 cgggacgaga atgccgcccg cacatcctat gtcgagtacc tcgcttccat tgcgaagtag
     3781 ggaagcgaga cgggtgaatt ccgcttcatg gtggaaggag ttcgtcttcc tcgccgtcca
     3841 ctcgttcgcg tgcgagtcgt agtagcgctt cgtcgctcgt atttcggatg acggcctact
     3901 tcgtcttgag gtggacatag atgtcttcga gagcggtcac tgcgtttccg gcggaaatgt
     3961 tgttttgatg gtacttgatg ttcgtacaat cgccggctgc ccatacgccc gatgtgctcg
     4021 tcgctccggt ccatgggtct atcttgatac gacccacctc atcgagttcg acgagaccgg
     4081 cgacgcactc ggtcgtgggg acgaggccta tctctacgaa gatgccggcg acggggagcg
     4141 tctgcgtcgt gccctccgcg tcgtcataca cgacgccggt cacgaatcgg tcgccgacga
     4201 cctcctttat cttcacgttc ttcacggcct tcatgttcgg gtgcgcgagc accttttcga
     4261 ccgtaacggg gtccgctcgg aaattctcgc tccggtggag gagcgtcacg ctcttcgcat
     4321 acgcgaggag ttgagccgcc gattcgaacc ccgcattacc gccgccgatg acggcgacgt
     4381 cctgtcccgc gaagagagga ccgtcgcagg acgcgcagta cgtgagaccc ttttggtcgt
     4441 acgcctctgc gccgggcacc tcgagctttc ttcgcgtcga gcctgtcgtt atgaggagcg
     4501 ttttcgcctc gtaggaatcc ccgttgtcga gcgtcacctt gaaatgcgca tcagcgagct
     4561 tctcgacctt cactgccgta cggcccgaga ccacggtaag gacatcgtct gcgtacgcgc
     4621 ggaggtgccc ctcgaggttc ttcgcgaggt ccgaaccgga tatgttcttc gtgccgatcc
     4681 agttctggat atcggtcgag acggtgctct ggccacccca ttccttcgtg atgaagacgg
     4741 tcgcaagctt tttccgagac gcgtagacgc ctgcggcgac gccgccgggg cccccgccga
     4801 tgatgatgag atcgtgcata tgagttcggt acgagtaaag tccgtccatt gtatcccaga
     4861 aggtagcaaa caccaaaaat gccggcatgg ccggcatttg taaacacaaa aattatttta
     4921 gagacggaag gcaggaacgg cctcttacag aggcagtcca ctcattggct cttttgcttc
     4981 gtgtttcctc gcaaaagtgc tcaatgagtc cgcggtgccc tcatttcgtc caccaaagca
     5041 caattacttg agctttgatc tcctcaagaa agcgggcacc gcgccccagt cgtcgtccgg
     5101 ctcctcatcg atcatgtcga cgggcttcgg ctccttctga acgggcgctc gttcttcgcg
     5161 aacttctctc gcgacgacgg gctcgtcctt caccggttcg ggcttgccgc cgccgataga
     5221 attgaatatg cgcccgcgga ggtccttctc ctcggtcgcg ggaacggtct gtcgctcctg
     5281 gatgcgcatc gaacttggga cgccatggag ctccatctcc gggaacccgg tcgcgatgac
     5341 ggtgacgcgg acctcgttct tcttaagctt gtcgtccttg atcgcaccga agatgacctt
     5401 cgcgtgcggg tcgatggact cggtgatgac gcgtgccgcg tcctgtatct cgagcatgcc
     5461 gaggtcgtcg ccgcccgcga tcgcgaagag aacgcccttc gcgcccgtga tcgatacctc
     5521 gaggagcgga gagttgatcg cgcctcgagc cgcctcttca gcgcgcttct cgccggcgga
     5581 gacgccgacg cccatgagcg cggatccggc gttctccatg acgctccgga tatcggcgaa
     5641 gtcgacgttg atgatacccg gcatcgtgat gaggtccgag atgccctcga ccgcctgctt
     5701 caaaacgtta tcgcagagct ggaaggccgc catgacgctc gtctcccggt cgacgatcgc
     5761 gaggagccga tcgttcggga tggtaatgag ggcgtcgacc tccttcttca gttcctcgat
     5821 gccctggagc gcgagccgca tgcgctcctg tccctcgaag aggaacggct tcgtgacgac
     5881 gccgacggtg agcgcgccgg tctcccgtgc gatcttcgcg acgactggtg ccgcaccggt
     5941 gccggtgccg ccgcccatgc cgcccgtgat gaagatcatg tccgaaccct tgatcgcgtt
     6001 cgcgatctcc tcgcgcgtct cctccgccgc gcgacgtccc atgtcggggt tgccgcccgc
     6061 gccgagaccg cgcgtgaggt tcttgccgat gtttatcttc tttttcgcga gcgagtggtg
     6121 gaggtcctgt gcatcgctgt tgatggcgat gaactcgacg cccttcacct tcgcgccgac
     6181 catgtagttc acggcgttgt tacccgaacc tcctacgcct acgactcgta tccgtgcaaa
     6241 cgattcgacg tcggtctcga tcttttccat agctaaataa atttgcgagc tacctccacg
     6301 gccgccccct gctaatgctt gcgagtatca tacaggcgcc aaaaacatct gtcactatat
     6361 tgacaaaagt gagtttcgtg cgattgccga acgaccgcca tgtggtcgtt tacggcagga
     6421 attgcttgaa ccaattgagg agcgtgttgc cggtcgtccg cgcgaggttg ataccgctct
     6481 cttcttccgt accggtaata ccccagatgc agaggccgta gccgacggcc catgtcgcgt
     6541 cgcgtacctt gccgttttgg cccggatcga ggagcgctat cttcgaggga aggcggagcg
     6601 agacgcgtgc gaggtcttgt atcatcgcga tcgacgagcc gccgccggtg aggatgatcc
     6661 cggcgggaag aaggccgtct cgcttgatct tccggaggtg cgcgtcgatg agctcgaaga
     6721 tatcggtgag acgcgcgctc acgatctcgt cgagcttctt cttcgagtag ggtgtctgcg
     6781 acatgccgcc gcgctttatc ttctctgctt cgtcgagggt gaccttgagg ccgagcgcga
     6841 tgtcgttcgt gatgtcgttc gcgccgatgg ggaagacctt gagggagacg gggatgctgt
     6901 tctcgaagac gacgatggag acggtctccg caccgatgtt cgcgaggacg cagccagcgc
     6961 gtttctgggc cttcgtgagc atgacgaggc tccctgcgat gggcgaggcc atgacgtcat
     7021 cgaccttgat gccgatcgac tcgatggcgc cgacgaggtc gttgaggtgc tgttcgaagg
     7081 ccgtgatgaa gagaacgtcg acctcgagct tcgtgccctt gaggtcgtgc ggtcggccgt
     7141 ggaccttctc tccgtcgacc gtgtaggaga gcggtatcgc gtggatgatc ttccggttcg
     7201 gtatcctgtc ctggatggac tcttcgctcg cctggatggc cttgtcgatg tcgacttgcg
     7261 tgatgaccga gtcggcgcgt gaggtgatgg cctcgccgcg cgagtgtatc tcgtcgacgc
     7321 cgatgccgcc catggagata tacgcgcgtt tcaccttcac gccgctcgcc ttctccgctt
     7381 gcgcgaccgc gt
//
LOCUS       DASTSP010000074         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1728717, whole genome shotgun sequence.
ACCESSION   DASTSP010000074 DASTSP010000000
VERSION     DASTSP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728717"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..795
                     /locus_tag="VFS75_03430"
     CDS             385..795
                     /locus_tag="VFS75_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677739.1"
                     /translation="MVPVPAVADTAYGHIETNDATVVEAAPRKGSLESDVEKAVRSYF
                     KDVPVLIQIARCESTFRHTLPDGSILRGKVDPADVGVMQINTRYHGAKAKSLGLDLTN
                     LYDNMKFARDLYEEQGTKPWKASSACWSNSIAMR"
     gene            877..1344
                     /locus_tag="VFS75_03435"
     CDS             877..1344
                     /locus_tag="VFS75_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011613744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU4677740.1"
                     /translation="MRYWLMKTEPSCYSIDDLKREKVGMWDDVRNYQARNFMRDMKRG
                     DLVLFYHSSTDPTGIVGVAKIAREAYPDPTQFNRRSYKYDPRSSKNAPRWDAVDVRFV
                     EKFGEPLTLAELKDDPVFSDMLVTRRGNRLSVMPVSETHAKRVLNLRSKGKTP"
     gene            1341..1571
                     /locus_tag="VFS75_03440"
     CDS             1341..1571
                     /locus_tag="VFS75_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEU4677741.1"
                     /translation="MKAIPGKRYRHYKGGEYTVLAVGRMETSPDEEYVVYRMEYDTND
                     LPKGTVWLRARAVFEERTVVDGVEVERFTELA"
     gene            1568..2338
                     /gene="murI"
                     /locus_tag="VFS75_03445"
     CDS             1568..2338
                     /gene="murI"
                     /locus_tag="VFS75_03445"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU4677742.1"
                     /translation="MKIGFFDSGLGGLTILKAVRERMPKYDYLFFGDTANVPYGEKSE
                     SEIFELTKAGVVRLFNEGAVIVIVACNTASAESVRRLQDSILEGVYKDRKILGVIIPT
                     VEELEALGAKHVLLIGTERTVKSEKYTRELMRLAPGVVLDSRATPALVPMIEAGDIAG
                     AAHVALGLIDHMKGRVDTLVLGCTHYTVLKELIRKAHPDLRVISQDDVIPIKLEEYLG
                     RHAEIKRKLSRRGTVTVMESKDAPVSEFKRDLLKSLGE"
BASE COUNT          573 a          686 c          725 g          491 t
ORIGIN      
        1 gcccgcccat catggacgcg gccctcaagg acgagggggg tctctacggt gcgatggttt
       61 tgatgaagca aaaattagaa cacgaaaaag aataaacgtc aagtaatttt gccatttaca
      121 tggcgcaaaa taagaccatc gacgatattt gacgcactgg gagtaacatt gtgtaatttg
      181 caatttgggg ggccggtggt ataattgtca tcgacggttt aggccgtttt ttgtatagcg
      241 aaaggctata caagtagcgc acgttcggtt cgtacacgtt ctgtctaggc aggctggcac
      301 ggacccctcg cgcgctattt tgttggataa gacacttggc ttatattatc gagcttaatc
      361 acagcggtca tcgctgtatc gaacatggtt ccggtgcccg cggtcgcgga caccgcgtac
      421 gggcacattg aaacgaatga cgctacggtc gttgaggcag ctcctcggaa ggggagcctg
      481 gagtcggacg tcgaaaaggc ggtccgttcc tacttcaagg atgtaccggt tctgatccag
      541 atcgctcgct gtgagtcgac gttccgacac acgctgcctg acggatcgat cctccgagga
      601 aaagttgacc ccgccgacgt cggtgtaatg cagatcaata cgcgttacca cggcgcaaag
      661 gcgaagtccc tcggtctcga tctcacgaac ctctacgaca acatgaagtt cgcgcgcgac
      721 ctctacgagg aacagggcac gaagccctgg aaggcctcgt ccgcctgctg gtcgaactcc
      781 atcgcgatgc ggtaacctac cgacgaacga gcctcccttc ggggaggctt tttcgtgcgt
      841 ggcgaaaggg agggggacag ggtatcatac gtccgtatgc gctactggct tatgaagacg
      901 gaaccgtcct gctattcgat cgacgacttg aagcgcgaga aggtcggtat gtgggatgat
      961 gtacgaaact atcaggctcg gaacttcatg cgcgacatga agcggggaga cctcgtcctc
     1021 ttctaccatt catcgacgga cccgacggga atcgtcggcg tcgcgaagat cgcgagagag
     1081 gcgtaccccg acccgacgca gttcaaccgt cgctcgtaca agtacgaccc gagatcatcg
     1141 aagaacgcgc ctcgctggga tgcggtcgac gttcggttcg tcgagaagtt cggcgaaccg
     1201 ctcacgctcg ccgaactcaa agacgacccc gtgttctccg acatgctcgt cacgaggcgc
     1261 ggcaaccgcc tctccgtcat gcccgtctct gaaacgcacg cgaagcgagt cttgaacctt
     1321 cgttcgaaag gtaaaactcc atgaaagcga taccaggtaa acgctatcga cattacaagg
     1381 gaggggagta taccgtcctc gcggtcgggc gcatggagac gagccccgac gaggaatacg
     1441 tcgtctaccg gatggagtac gatacgaacg atctcccgaa gggaaccgta tggctccgcg
     1501 cacgggccgt gttcgaggaa cgtacggtcg tcgacggtgt agaggtcgag cgattcaccg
     1561 aactggcatg aaaataggat tcttcgattc ggggctcggc ggccttacca ttctaaaagc
     1621 ggtgcgcgag cgcatgccga aatatgacta cctcttcttc ggggacacgg cgaacgtacc
     1681 gtacggcgag aagagcgaga gcgagatatt cgagctcacg aaagccgggg tcgtgagact
     1741 ctttaacgag ggtgccgtca tcgtcatcgt cgcgtgcaac accgcatcgg ccgaatccgt
     1801 gcgtagactc caggacagca tcctcgaggg cgtgtataag gataggaaga tactcggcgt
     1861 catcatcccg accgtcgagg agctcgaggc cctgggcgcg aagcacgtcc tcctcatagg
     1921 aacggagcga accgtgaagt ctgagaagta cactcgtgag ctcatgcgcc tcgccccggg
     1981 cgtcgtcctc gactcgcggg cgaccccggc cctcgttccc atgatcgagg ccggtgacat
     2041 cgctggcgcg gcgcacgtcg ctctcggcct catcgaccac atgaagggga gggtggacac
     2101 gctcgtcctc ggttgtacgc actatacggt cctcaaagag ctcatccgga aggcgcaccc
     2161 cgaccttagg gtcatctcgc aggacgatgt catacccata aagctcgagg agtaccttgg
     2221 ccgtcacgcg gagattaagc ggaagctctc gcgacggggg accgtgactg tcatggaaag
     2281 caaagatgcg ccggtgtccg agttcaagcg agacctcctt aagagcctcg gggaataggc
     2341 catatcgaaa taccctgcgt gtcgcacaat atatcttttg cgacctatta tatactgggc
     2401 cacttcccta catatcgaaa tttaaaaaga ccgcacacgc ggtctttatc tcaacaaatc
     2461 ggtggagtga gggtc
//
LOCUS       DASTSP010000075         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1964049, whole genome shotgun sequence.
ACCESSION   DASTSP010000075 DASTSP010000000
VERSION     DASTSP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964049"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VFS75_03450"
     CDS             complement(<1..1260)
                     /locus_tag="VFS75_03450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU4677743.1"
                     /translation="MRSLASYPRAILHVDGDSFFASCAIAVDQSLRGKPVVTGKERGI
                     ASSMTYDLKAMGVTRGMRLSEIRKICPEVVIVPSDYETYSLFSMRMYDIVRRYTPEVE
                     EYSIDECFADLTGMRRALRMSYEDVALRVKRELDGELGMTFSVGLAPNKVLAKVASKW
                     RKPSGLVFIPGRSIEAYLAELPVGKVWGIGPQTAAFLNAHGVLTALEYARKPREWIDA
                     RMSKPYREIYEELNGEFVYPLTTEAKHDYQSISKTKTFTPPSADESFVFSQLSKNIEN
                     ACIKARRHGLAPKKFFYFLKTQEFRYRGLELSLAAPLSAPHELVRLARESFHKVFKRG
                     TPYRATGAVLMDLGPEHALQLDLFGSVASAEKWKPVYATVDGLDERFGKHTVYLGSTW
                     KAMHTKAHENERGDAPARAKELLRGEND"
     gene            complement(1262..1822)
                     /gene="lexA"
                     /locus_tag="VFS75_03455"
     CDS             complement(1262..1822)
                     /gene="lexA"
                     /locus_tag="VFS75_03455"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU4677744.1"
                     /translation="MTPLQKIREFYETKRRMPSYQELMGLLGYRSKSAAHYAVKKLIA
                     EGGIAKDKTGKLVPAKFDAGVPLLGVVEAGFPSAAEEELLDTMTLDEYLVTNKQATFM
                     LHVKGDSMMDAGIMAGDLVLVERGRTPKHGDIVIAEVDGEYTMKYYRKSREKVWLEPA
                     NKRYKPIYPTAELKVEAIVTAVVRKY"
     gene            complement(1898..2143)
                     /locus_tag="VFS75_03460"
     CDS             complement(1898..2143)
                     /locus_tag="VFS75_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677745.1"
                     /translation="MRDIQTVPDLYNEFDSIVQLSGQLVRVQAGGRFGIFSLETMEEV
                     VPVECEEVVPLDGDRFRVTISKKRGIFSATKNEFAWE"
     gene            complement(2789..2871)
                     /locus_tag="VFS75_03465"
     tRNA            complement(2789..2871)
                     /locus_tag="VFS75_03465"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2835..2837),aa:Tyr,seq:gta)
     gene            3114..3938
                     /locus_tag="VFS75_03470"
     CDS             3114..3938
                     /locus_tag="VFS75_03470"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEU4677746.1"
                     /translation="MSKRSFIAFVLGFALVVLPGVALANIVPCDGPNCRACDLVQLGQ
                     NALKFFIDAMALIIALVFAIGGLKMVLAGGNEGQIASAKSMMTNSVIGFILLLSSYLI
                     IDTILKTFVTGADLGPWNEVKCESAPPVTPTGTPPPVSPVTPAAGTYTDADARAALAA
                     AGITVNKTEAQGTSLNGMRTATIQDAIDLKHDCDCTVVITAGTESTGGHAEGTYSHES
                     GYKYDMRVNDRLDGYIYSSYDKIKTRSDGAEQYRNPKDGTIYAREGDHWDVLVPPS"
BASE COUNT          828 a         1133 c         1148 g          860 t
ORIGIN      
        1 gtcgttctcg ccccggagga gctccttcgc gcgcgcgggg gcgtcgccgc gctcgttctc
       61 atgcgcttta gtgtgcatcg ccttccacgt cgagccgagg tagaccgtgt gcttcccgaa
      121 gcgctcgtcg agaccgtcta ccgtcgcgta gacgggcttc cacttctccg cggaggcgac
      181 cgacccgaag agatcgagct ggagcgcgtg ctcgggccct aaatccatga ggaccgcgcc
      241 cgtcgcgcgg tagggtgtgc ctcgtttgaa caccttatga aacgattcgc gggcgaggcg
      301 gacgagctca tggggcgcag agagcggtgc ggcgagcgag agttcgagac cgcggtagcg
      361 gaactcctgc gttttgagga aatagaagaa cttcttcggg gcgagtccgt gccgtctcgc
      421 cttgatgcac gcgttctcga tgttcttcga gagttgcgag aagacgaacg attcgtcggc
      481 cgaggggggc gtgaacgtct tcgtcttcga aatgctctgg tagtcgtgct tcgcttcggt
      541 cgtgagagga tagacgaact cgccattcag ttcctcgtat atctcgcggt agggtttgga
      601 catgcgtgca tcgatccatt cgcgcggctt tcgggcgtac tcgagggcgg ttaagacgcc
      661 gtgggcgttg agaaacgcgg ccgtctgcgg accgatgccc cataccttcc cgacgggcag
      721 ttccgcgaga tacgcttcga tggaacggcc gggaatgaag acgagaccgg aaggctttcg
      781 ccacttcgag gccactttcg cgaggacctt gttcggcgcg aggccgaccg agaaggtcat
      841 gccgagctcg ccgtcgagct cgcgcttcac gcggagggcg acgtcctcgt acgacatgcg
      901 gagcgctcgt cgcatgccgg taaggtcggc gaagcattcg tcgatgctgt actcctcgac
      961 ctcgggcgtg tatcttctca cgatgtcgta catgcgcatc gagaagaggc tgtacgtctc
     1021 gtagtcggaa gggacgatga cgacctcggg gcatatcttc cgtatctcgg agagtcgcat
     1081 gccgcgggtc acgcccatcg ctttgaggtc gtacgtcatc gaggaggcga tgccgcgctc
     1141 tttgccggtc acgaccggct ttccgcggag cgactgatcg accgctatcg cgcacgaggc
     1201 gaagaacgag tcgccgtcaa cgtggagtat cgcgcgcgga taggaagcga ggctccgcat
     1261 actagtactt ccggacgacc gcggtcacga tggcctcgac cttgagttcg gcagtgggat
     1321 aaatgggctt gtaccgcttg ttcgcgggct cgagccatac cttctctcgt gacttccggt
     1381 agtacttcat cgtgtactcg ccgtcgacct cggcgatgac gatgtcgccg tgcttcggcg
     1441 ttcgtccgcg ctcgacgagg acgaggtcgc ccgccataat gcccgcgtcc atcatcgagt
     1501 cgcccttcac gtggagcatg aaggtcgcct gcttgttcgt aacgaggtat tcgtcgagcg
     1561 tcatcgtatc gagaagttcc tcttcagccg cggaagggaa gcccgcctcg acgacgccga
     1621 gaagcggcac gccggcatcg aacttcgcgg gaaccagttt tccggtcttg tccttcgcga
     1681 tgccgccctc ggcgatgagc ttcttcaccg cgtagtgcgc ggcgctcttc gagcgatacc
     1741 cgaggaggcc catgagctct tggtagctcg gcatgcggcg tttcgtctcg tagaactcgc
     1801 ggatcttttg gagcggtgtc atagagcaag tatatagaac gttcgttcta atcgcaaggc
     1861 gaaaagaact gccctcgggg agagcagctt ttgggtttta ttcccaggcg aactcgtttt
     1921 tcgtcgcgga gaagatgccg cgcttcttcg atattgtcac gcgaaagcga tcgccatcga
     1981 ggggtacgac ctcctcacac tcgaccggaa cgacttcttc catggtctcg agcgaaaaga
     2041 taccgaaccg tcctccagcc tggacccgta cgagctggcc agagagttgc acgatggaat
     2101 cgaattcgtt gtagaggtca ggtactgtct gaatgtcccg catgcgcgac cctttccctt
     2161 attatgtcct gcgcgttcag tatatcactc acgcattaac ttctcgtgaa gatttcagta
     2221 cacgatgagc gtgacgaccg cggagagaag catgaagaag cccatgatgt agaggatgac
     2281 cttcataccc tacgagtagc atgacggcgg tgatggagag atgaacgaaa aacgactccg
     2341 tcgtcgacgg agtcgttttt atttagaggt gagcggacca aacgatggtg cccgcaatga
     2401 atgaacaggc atatcctggg ccgcacattc tccagagaag aactatgacg actatggata
     2461 cgatctgacg cgggaccgcc ttcctatcga acgttatcac ctcgcgaagc atagaaccct
     2521 ccttcaaaga ctggcaggat agtacactat atttctatat ccgtcatgcg aacgattacg
     2581 cggatacaaa agagcaggca aggtcttcga atcctccaac agagtgtcaa cagattcgat
     2641 ccttacagga tcagtacacc ttgtcactat tttgtttcgc ttcactcaca aaatagtagg
     2701 caaggtcttc gaatcctcac gcaagctctc ccagagcttg ctcccggcac ccaaatttaa
     2761 aagcggccag aagccgctct taaatttgtg cgccggggag gattcgaacc tccgtaggcc
     2821 gaagccgaca gatttacagt ctgttgtaat tgaccactct accaccgacg cgagaatctt
     2881 gattttgcaa gaacgcttcc gcttctatgg gcgttcgacc gcccttccac cttgagtctg
     2941 ttcgcatatg ctcacaacga ctcatctgct cacatccgtt cgcagaccgg ataaatcgta
     3001 aagaagttta cgatttctcc actctaccac cgacgcattt ttgaacaggg tataaagtac
     3061 cacaattcac cgtttcgcgg aagggtagtg tctccctatg ctactattcg aatatgtcga
     3121 agaggagttt catcgcgttc gtattgggat tcgccctcgt tgtcctccct ggcgtcgcgc
     3181 tcgcgaatat cgtcccgtgc gatggtccga attgccgcgc ctgcgatctc gtccagctcg
     3241 gtcagaacgc gctcaagttc ttcatcgatg cgatggcgct catcatcgcc ctcgtgttcg
     3301 cgataggcgg tctcaagatg gtcctcgcgg gcggaaacga aggacagatc gcgagcgcca
     3361 agtcgatgat gacgaattcc gtcatcggtt tcatactcct cctctcctca tacctcatca
     3421 tcgacacgat attgaagacg ttcgtcaccg gtgccgacct cggtccgtgg aacgaggtga
     3481 agtgcgaatc agcgccaccg gtcacgccga cgggaacgcc tccgccggtg tcaccggtga
     3541 caccggctgc tggaacgtat accgacgctg atgctcgtgc cgcgctcgcg gcggccggta
     3601 tcaccgtcaa taagaccgaa gcgcagggga cctccctcaa cggcatgcgg acggccacga
     3661 tacaggacgc catcgaccta aagcacgact gcgactgcac cgtcgtcata acggcaggaa
     3721 ccgagagcac gggcggacac gccgaaggca cgtacagcca cgagagcggg tataagtacg
     3781 atatgcgcgt gaacgacaga ctcgatgggt acatttacag tagctatgac aagatcaaga
     3841 cacggagcga tggtgcagag cagtatcgca atccgaagga tggtaccatt tacgcgcgtg
     3901 aaggggatca ttgggacgtt ttagtcccac ctagttgacc cgtcgtagcc tttctcgtag
     3961 gatggtttg
//
LOCUS       DASTSP010000076        24133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_178860, whole genome shotgun sequence.
ACCESSION   DASTSP010000076 DASTSP010000000
VERSION     DASTSP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 24133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24133
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178860"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFS75_03475"
     CDS             complement(<1..352)
                     /locus_tag="VFS75_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677747.1"
                     /translation="MAAVVSVIDTLFDRVVDVSVRVVSLALPRHGTRVTTEPAYETPS
                     EEEAELADDGPHTFRSIEEILHTERREPAVAAKNTIMYARSIAVPVFRNPTVEFDSEL
                     GVIPYGEMVMMLEPR"
     gene            complement(422..1090)
                     /gene="nth"
                     /locus_tag="VFS75_03480"
     CDS             complement(422..1090)
                     /gene="nth"
                     /locus_tag="VFS75_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4677748.1"
                     /translation="MTEKQREERRKRVRAMNRVLRRLYPEPKIALDHENDWELLVAVM
                     LSAQCTDKRVNATTAKLFKKYRTVDDYARVSQGALERDIFSCGFYRNKAKNIRAAAAM
                     VRDEFGGDVPRTMEELLRVPGVARKTANVILSNAFGVHEGIAVDTHVRRFAIRFDLSD
                     FRDPVRIERDLLSIMPRSEWWGFNHRLVHYGRDYCPARRHDCVGHPLTKVYPKAAEIW
                     PRAR"
     gene            complement(1153..1359)
                     /locus_tag="VFS75_03485"
     CDS             complement(1153..1359)
                     /locus_tag="VFS75_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015473620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEU4677749.1"
                     /translation="MNNKLLHIVAFVLLVVGGLNWLLVAFDINLVEVIFGTGTVTQVV
                     YILVGLSAIYEVITHKGHCTLCNS"
     gene            complement(1439..2545)
                     /locus_tag="VFS75_03490"
     CDS             complement(1439..2545)
                     /locus_tag="VFS75_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677750.1"
                     /translation="MKRTIIIALGLLFALPAFAAGPIIRTGENISIDAAQVLSGDFYG
                     FAPSVTVSGDAKDDAYVAGGTVTVNAPIGSDLAALGGTVQVHGDVGDDLRVVAGDVTL
                     GKSVKGDVVVLGGKLTVLSTASVGGDVIFMGGDLTVEGPVAGSVHGSAETARINAKVG
                     GDVSLETKSNLTLGDNADIAGDVSYESESELTRAQNAHVGGSVHRSDVQPKDVPASVA
                     RTYLAGVFMLVFATMSLYFVGRAKVARVVEVASTSLGQSGLIGIASLILVPFVSSILI
                     VSVIGGVLGVALFVAYLTALIGSLLTAGVLVGHYVGVHVLKRPPFSLPAAFLGSVLFA
                     LLPLVPYVGGLAAFALAMVTFGAFMTLVYRAIRG"
     gene            complement(2582..2968)
                     /locus_tag="VFS75_03495"
     CDS             complement(2582..2968)
                     /locus_tag="VFS75_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677751.1"
                     /translation="MNIGKLALIAAIAFLLIAIGAGVFVWYTVQTLDRETHGVTATST
                     PAAATGTPEAPTVTEPITVKASDLSKTQQKALDAVGLGDASITITPEMVACAEDAIGA
                     DRVNDLMGGAAPTPLESVKLLPCLKK"
     gene            complement(2981..3151)
                     /locus_tag="VFS75_03500"
     CDS             complement(2981..3151)
                     /locus_tag="VFS75_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677752.1"
                     /translation="MDIIRFILPFLFVRNWYDGEWELSRPRLYALLILIALVAAVLVA
                     AYFMQSPIVYHG"
     gene            complement(3166..4176)
                     /locus_tag="VFS75_03505"
     CDS             complement(3166..4176)
                     /locus_tag="VFS75_03505"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU4677753.1"
                     /translation="MRKRLLKVLIGLVLLLGVVGLVLFALARKEAPTTITYGMSFSVP
                     YAKELGLDPDDVFDALIDDMGVRNFRLAAHWPLIEPKRGQYDFTWMDRDIEKAEAADA
                     HVIFAVGRRLPRWPECHIPDWAKDMTWDEQKAEIRDYLTTVVNHYKDSSAITYWQVEN
                     EPYLNLFANEYCGDLDEDFLKEEIALVHTLDPTRPVLVTDSGNLGTWRGAYENGDAFG
                     TSVYVYFWNPQLGQFKTVLPPWFYRAKEKVMALLYGEKKTFLIELELEPWLLTPVVDA
                     PLETQFSRMDLEKFDEIIDYAKGTRYDTQFLWGGEWWYWLKKQGHPEMWERGKQLFSS
                     ST"
     gene            complement(4176..5108)
                     /locus_tag="VFS75_03510"
     CDS             complement(4176..5108)
                     /locus_tag="VFS75_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677754.1"
                     /translation="MSWILLAAGAQFINALVAVVDKRIVSDDKILPRPFVYAYYTLLI
                     SGAWVLVYLVSFVPVIGGFHIPSFENVYRPTLEVVALSFLAAYCFFIGLVSMFTAFRC
                     ADAADVVPVVGATSAIGSLGLGYFFLHGAISSNFLLGITLLSIGTYFVSRYHFGWKTA
                     LTSIHSGLFFAFHYVSMKGLFNVTSFDDGFFWSRIAFMLFALTLLLVPAYYDKIKVHT
                     KKVTRATGTLILANKVLAGVATILILKATELGDVAVVQALGGLQFVFILFIGIFFTLK
                     PRRGHVGEMYRHDTILQKTLFVAIISLGFLVLFT"
     gene            complement(5113..6351)
                     /locus_tag="VFS75_03515"
     CDS             complement(5113..6351)
                     /locus_tag="VFS75_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU4677755.1"
                     /translation="MNETNNVKEFPRGFYWGAATASYQVEGGIENTDWAKAAREGRVP
                     SCGIACDHYHRYEEDFDIAKSLGHNAHRLSIEWARIEPEEGKWNEEAIAHYRKVLTAL
                     TVRGMKPYITLWHFTLPLWFSESGGFERMDSPEVFARYCAYVVSQLGDFCGDFSTMNE
                     PNVYGSNGWLRGSWPPFKRFSLTDLVSITNSGRTFESKAARGLKPLFLYFRVMRNLAR
                     AHNRAYDEIKKVARHVDVSVVKHVILFHANWNPVNKLVAWVANRFWTHRFMRRVYRKC
                     DSIGLNYYFHRKFGDTATYAKTDMDWDIYPEGIEAALCMLARYKRPLYVAESGIADAK
                     DDRRADYITRQVRAVSRAIQRGVDVRGHMYWSLLDNYEWALGFEKRFGLVEIDYKTLT
                     RVIRPSAYVYKEICERNAVE"
     gene            complement(6415..6909)
                     /locus_tag="VFS75_03520"
     CDS             complement(6415..6909)
                     /locus_tag="VFS75_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677756.1"
                     /translation="MDTIRIVPLGPVAEAADRLMAPLMVLLSGHVGESPQRTHRWNNT
                     KLRSSDITHLDPFVMVHEKGRQSRARWWGKLPVFHMPVVGWKDYIVLRPVAPEYPWHV
                     GWITDDVIGVSRIEVRGQVRLLIGPGDVTFFGVDHAGAQVAVVKDGEGRLGDGSRFRT
                     VPLL"
     gene            complement(6958..7689)
                     /locus_tag="VFS75_03525"
     CDS             complement(6958..7689)
                     /locus_tag="VFS75_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU4677757.1"
                     /translation="MKTLIFDIETVGEEWNSLDETTQSMLTRWVERTARSEDELEAGL
                     KDVREGLGFSPLTGRIVAIGLYDLERKQGVVYYEAEEGEGEGRTGEFALKPRSERDML
                     EAFWDGAKSYDTFVTFNGRGFDVPFMNLRSSVHGIRPSKDLMDGRYLYQQKYARHVDL
                     QDQLTFYGAMHKRPSLHLFCHAFGIESPKGEGVAGDDVAGLFKEKRFRDIAEYNSRDV
                     VATTALYEKWLETLAPRAFREELKN"
     gene            complement(7699..8037)
                     /locus_tag="VFS75_03530"
     CDS             complement(7699..8037)
                     /locus_tag="VFS75_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677758.1"
                     /translation="MIRTKHFLILVVATLALSSALAFPNPEVKADENRASAIFATPKA
                     SYAATVVTNEPSSEWRDAFIAKVRAALRRDELSRPVVSPAPAPLPTPEPLPLLPASAT
                     ETPGTTTEAL"
     gene            complement(8070..9344)
                     /locus_tag="VFS75_03535"
     CDS             complement(8070..9344)
                     /locus_tag="VFS75_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4677759.1"
                     /translation="MKAERNIIVYVMTLPPGAIESIRAYEKREKRKFRVMLIKDVHHQ
                     REKGRDDYKGLDILVECDFSVPARIAEALAPYQNELCAITCRAESYMARFVQVIPHVP
                     YLRTPTTESLRWATDKLEMRRRFKLLAKRYSPKYAIVRANTKEERRRVIGRIKFPMIV
                     KPANLAQSLLVSVCYHEDELKEVLAQSFKKIEKIYAENGRIETPKMMVEEFMEGDMYS
                     VDAYVNGLGRVYFCPMVRVFTRKNMGHDDFSNYIHMSPTGLKKKSVEAAHMAATAGIH
                     ALGLRNTTAHVELMKLDDEWKLIEIGPRVGGFRHKLHELSCDIDHSMNDILIRLPKRP
                     ILPKKCKGFAATLKWYPMKEGRITKLKGIKKCQELKSFAEISVLKKVGDLCKFAKNGG
                     KAVFTLTLYNDERAKLLADIRRAEKLIDVQIV"
     gene            9440..10336
                     /locus_tag="VFS75_03540"
     CDS             9440..10336
                     /locus_tag="VFS75_03540"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677760.1"
                     /translation="MKQRLYLINNKVGDASFRDAVRLVAREHGTSVSFLFANRFAFHC
                     PRGRRDVSLYHDGKRVPFRREDRVFVRAWGAHPTATALLSIVLRSLGIRFVDERVHVS
                     HGIRSSKLAHAFILKEAGIASPNTWVATPRSFPTVRARIEKDLRFPLVVKTKGSKGKC
                     VWKCDTRAALDARVAKLRKSELILFQEYVPNESDIRAIVFDGALIGGIERSSKDGFYN
                     NIAKGASARLVPLSREETRIAKKAAHVVGLDLAGVDIVRSAHGPLVFEVNKAPDISGF
                     NDAAGFNIAEAIFRRYLSRARS"
     gene            10413..11207
                     /locus_tag="VFS75_03545"
     CDS             10413..11207
                     /locus_tag="VFS75_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677761.1"
                     /translation="MKIKYASAHRWLKPVPNDALASFGKNSRARLRSALPKIAALPVR
                     NEIAKLDEAFLERFLPRYEARIGEKRHPVVFDVRAKTLGSAKADRYYAMSLYEGDRFL
                     GGTIFAIRKDRLAVAYRTYENAWHGARLQANPSLYVEYCLNIHAKEIGVAYIVHGKDR
                     NPYGVNSNIGLAAFKLAAGCHAEIPRKFEIGELDTETCAEDALVLEYPGDEPRAPIRR
                     AYLVTTAETEAAWTQVVKYPHLLSVATIYRTKRPEDALLRLGTNVA"
     gene            complement(11209..11562)
                     /locus_tag="VFS75_03550"
     CDS             complement(11209..11562)
                     /locus_tag="VFS75_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU4677762.1"
                     /translation="MEKEKKRTNGVHSETKTYPRPAWVKKSVATEEELCPDCFKVVGV
                     QSRYKLVCLLGKAKKGETVGKLTEQLGLKQPTITHHLNILRSVNAVDVEDRGRERVYK
                     LNREAHCFDECKIPY"
     gene            11680..13830
                     /locus_tag="VFS75_03555"
     CDS             11680..13830
                     /locus_tag="VFS75_03555"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HEU4677763.1"
                     /translation="MPQTTRRELTHIIKRSGRRVPFSEGKIARAIEKALRATDAYRAG
                     APEKIAKAVIAELRERHKADETYVPSVEEVQDVIEMTLMEVGHHETAKAYILYREQHA
                     QIRRMVSTESIDLVDQYLARADWQVNENSNMAFSLQGLNNYIASEVSKNYWLNKIYPA
                     EVRKAHEEGDFHVHDLNLLSVYCVGWDLYDLLRRGFGGVAGKIQSKPPKHFRAALGQM
                     VNFFYTLQGEAAGAQAFSNVDTLLAPFIWYDHLSYDEVKQALQEFVFNVNVPTRVGFQ
                     TPFTNITLDLTVPSFYEKLRVIVGGKEQKETYGEFTREMDMFNRALLEVMVAGDASGR
                     VFTFPIPTYNITKDFDWENETIVGLWEAAAKYGIPYFSNFVNSDMSPDDARSMCCRLR
                     IDNRELMKRGGGLFGANPLTGSIGVVTMNLPRLGKTSKDEAEFLEKLGRLMDVAKESL
                     EIKRKVLESMTDKNLYPYTKYYLDTVKTGFGEYWKNHFSTIGLVGMNEAIRNLLGTSI
                     GTKEGIAFSERVLDYMRNRIADYQEETGNNYNLEATPAEGTAYRLALSDQKRFGDMIF
                     ANGTGDEVADPYYTNSTHLPVQFTDDMFEILELQDTLQTKYTGGTVIHFFLGERVRDA
                     SVVKKLVRKICETYHLPYFTITPTFSVCKNHGYLHGEVFECPTCHEPCEVYTRIVGYL
                     RPVDQWNKGKKAEYANRTEIESRALEAKEAALAV"
     gene            13827..14510
                     /locus_tag="VFS75_03560"
     CDS             13827..14510
                     /locus_tag="VFS75_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU4677764.1"
                     /translation="MRIGGLQHSSLIDYPGHIGAIVFTVGCNFRCPYCHNPELVDETA
                     DELTEEHVLEFLEKRRGLLEAVTITGGEPTMHADLPDFLRKVKGLGFLVKLDSNGTNP
                     DMLERVIGDGFVDYVAMDVKSPLSRYEATVGRPVNTEALRRSIALLIAGRIGYEFRTT
                     VVKSLLSKEDVRGIGEAIEGARLHYLQKFVSQKTLNPAFLKKTTYSDEEFEEMQKVME
                     EYVDTCTIR"
     gene            14515..15690
                     /locus_tag="VFS75_03565"
     CDS             14515..15690
                     /locus_tag="VFS75_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU4677765.1"
                     /translation="MRTIYLDHAASTPLRTEVLQAMLPYLEGAYGNPSSLHHKGREAE
                     RALRHARAKVAALLAVAPDEVVFTGSGTEANNLALLGTAEARSSRGRHVLVSPIEHPS
                     VLSPAEALARAGFDVEYLPVDAYGRIDAGDAYGRVRPDTILVSVMYANNEIGTVEPIA
                     ELGELLAALPETARPLLHTDACQALGQLPISPVDLHVDLMSLNSAKAYGPKGVGMLYV
                     RTGVRLAPRQLGGSHERGLRAGTENLAGIVGFSSALELAIPRAHETARRLSSLRNEFI
                     GMVRRLVPSATVNGHPTERLPNNVHLSVPYIEGESLLLLLDTYGICAATGSACSAHDL
                     APSHVLRAIGQSDELIHGSLRLSFGDETTRDDLAYVAEALAASVRRLTSVSPLPLHV"
     gene            15687..16142
                     /locus_tag="VFS75_03570"
     CDS             15687..16142
                     /locus_tag="VFS75_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU4677766.1"
                     /translation="MKTNLENTADVASCDSGESWLYSDVVKDHFFHPRNILLDDGYEC
                     DGVGVVGSPACGDMMVVWIRVDAGTGRISECKWRTFGCASAIASTSMMSVMATEEGGM
                     TLRDAKRLMPEAILERLGGLPDRKYHCSVLGHHALREAVLDYEEKRNEI"
     gene            16139..16855
                     /locus_tag="VFS75_03575"
     CDS             16139..16855
                     /locus_tag="VFS75_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4677767.1"
                     /translation="MSANEPVERTWVVRDARDEAPGVRSLYLQAVGERPRFIAGQYLT
                     VRLPGYEPVEGKSYSISSAEDDAQVRLTVKEAGPFSRAILAKRPGDLLRTSAPYGFFY
                     PERDAMSDLVFIAGGIGVTPAMGIIETLLNDGYAGSVTLFYSNKTEADIAFRARLESL
                     AAMHERFTLRNNVTREHPRDPSYEVGRLSGSSIVRSLSGSPTAADYFVSGSIDFTRTL
                     WKELRHEGVPQSSIYTEGFY"
     gene            complement(16859..17767)
                     /locus_tag="VFS75_03580"
     CDS             complement(16859..17767)
                     /locus_tag="VFS75_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677768.1"
                     /translation="MKKALTLGVAAFIVAAAVVYTPISASHASAAQTQTQFNLMYRFR
                     NQDRYITNYSQLYAFLEELIQYLNELQGQTSGTSEVDVATVSATNVMPYSALLRGRID
                     DFGSSNYADVWFEFGKDNGSALMRTSSERIDDNDNESFSRTVGGLAQNTSYRFRAVAR
                     DDNGDIDRGSFLSFDTGRTVSEDTPDVTTQSATDVTDDSAVLRGTVDMRDFDSGTVFF
                     VYGEDERLVDDVADYDRYSDIDEEGSDLQKVSVDSDLDGEESYTKRVSGLDNDRDIFH
                     RICVAYENDGDDRIECGSVRSFTTDR"
     gene            complement(17864..18484)
                     /locus_tag="VFS75_03585"
     CDS             complement(17864..18484)
                     /locus_tag="VFS75_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677769.1"
                     /translation="MRHLLTITLIVAVIFVAYFLTRPKANAPLSAPEGVESPVPLEND
                     ALTLYRDESLGLSFTYPSGDAGYVVRDPRIAEREAEKTIVLVHAADAAVYDTPAADGE
                     LPPTLSIVVYDNASKQFPGAWAESHTAQSGYGQKIGDAEDLVVGGANAIRYAADGLYR
                     ASVTVIAHGSRIYVVRGEYLDEASPIYRDYESLLDSITFVPAPGEF"
     gene            complement(18497..19030)
                     /locus_tag="VFS75_03590"
     CDS             complement(18497..19030)
                     /locus_tag="VFS75_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU4677770.1"
                     /translation="MRKVLGPSFYSRPAPVVARELLGKFLVRRVRGRELAGMITETEA
                     YEGLDDLASHASRGRTPHTEVMFGESGRWYVYLIYGMYSMLNIVTGPNGHPSAVLIRG
                     TDMVSGPGRLTRALAVTKTENANRATKETGLWIEDRGTRVPSRRIRNTPRIGVAYAGE
                     HWAAKPWRFVYDPDDVS"
     gene            complement(19032..20180)
                     /locus_tag="VFS75_03595"
     CDS             complement(19032..20180)
                     /locus_tag="VFS75_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU4677771.1"
                     /translation="MEPLASAIRPKTLDEFVGQEHLVGPGKPLRVAIEKKHLFSFILW
                     GPPGVGKTTLARLYTDALGGDFQELSAVSAGKDDIRKLVEGKERTMSLHPTVLFLDEI
                     HRFNKSQQDFLLPYVESGALTLIGATTENPSFEVISPLLSRCRVFVLNELSPEDMEKI
                     IVRVAKKEKVKMGKPAREWLIGMANGDARQAIAVIEATLRLYGKLTVENLTEALQSKF
                     LRYDKKGEEHYNTISAFIKSMRASQPDAALYYLARMIQAGEDPKFIARRMVVFASEDI
                     GLAQPTALVVANDVFRAVETIGLPECGINLAHGVVYLSEAKKDRRAYDAYMRAMKDAE
                     AYGNLPVPLKLRNAPTKLMKDLGYMKGYERYTKEDLFPEKLKGKKYLT"
     gene            complement(20216..21103)
                     /locus_tag="VFS75_03600"
     CDS             complement(20216..21103)
                     /locus_tag="VFS75_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4677772.1"
                     /translation="MQKIVPHLWFDSEAKEAAEFYVSVLGGEVKSVTLYPNAGQEIHG
                     RQAGSVMTVDFTVRGFEMLALNGGPVFNFTPAISITVECESAEEVDRLWGSLIDGGTA
                     LMDLDTYPFSPRYGWLRDRYGLTWQLIYHANAESHRMFPSLMFTGDVAGRAEEAMGFY
                     TKTFPRSEIGMIARYGAGAEPDVEGSVTHGIFVLEDQTFSAMDSAHPHQFNFNEALSF
                     LVRCEDQAEIDRLWSALSAHPEAEQCGWLKDRYGVSWQIVPANLGEILGDDDTAVVDR
                     RMAMLLSMKKIDIETLRAA"
     gene            complement(21140..21919)
                     /locus_tag="VFS75_03605"
     CDS             complement(21140..21919)
                     /locus_tag="VFS75_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU4677773.1"
                     /translation="MAHGPGKIHIGTSGWMYKDWGESFYPTDMKRGHLAYLARTFDTV
                     EVNSSFYHLPAKSTFEKWCRETPEHFVFSVKLSRYITHEKRIKSARVALERFMRHAEP
                     LRTKLGVVLVQLPPNLAYDKKVLGRFLQDLFVVTERNFRVRVALEPRHKSWMNCLLDP
                     DVRPLLWDRNIALVFPDSAKIPSFEPTIENITADFIYVRFHGPTSFASSRYGPRRLKP
                     WAERIARWSSGGFDVFVYFNNDVHGHAIVDARTLKRQLKLP"
     gene            22036..22488
                     /locus_tag="VFS75_03610"
     CDS             22036..22488
                     /locus_tag="VFS75_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677774.1"
                     /translation="MKYIFPIALIGMFPLLSSAATYHYVDQSGVVRDVEAPNAQTAIM
                     IAPDRDPHSGVAIDEGDIEPGMEVASVAGSDAGGSNGNGSDNGSLNGTGGPNTWHYVT
                     DAGLTATVEAPSPEVALMRATNIDPHSGVAIDEGMIEDGEFVPAAAHN"
     gene            22559..23206
                     /locus_tag="VFS75_03615"
     CDS             22559..23206
                     /locus_tag="VFS75_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677775.1"
                     /translation="MYYSNFQATLNELAASLPNIAGAVLIILIGWVIAVIVAAIVRRG
                     LQWMGADGLFEKTGVNRSPISSRTRVSNVVGAFVKWLIILGAVGIAAEIVHLDGVAAF
                     IGSLFAYVPNILVAAVILVVGFITADKVSALIGASTTFSSLSKDGRMLVTQAAKYGII
                     VFSVMAALTQLNIVPQLIEIAFAGLVFALALAFGLGGREHASELIARLKTKSKTG"
     gene            complement(23181..23552)
                     /locus_tag="VFS75_03620"
     CDS             complement(23181..23552)
                     /locus_tag="VFS75_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU4677776.1"
                     /translation="MVRKRVEGGISEKTFSERVVEIARAIPKGRVMTYGLIARYAGGG
                     AMAAQSVTSILGRAYDNGTRDIPFHRIVYADGRIWASDGRERRERMRKYRAEGIELDA
                     RDRIVCFAEVLFDPNPSSTLS"
     gene            complement(23588..>24133)
                     /locus_tag="VFS75_03625"
     CDS             complement(23588..>24133)
                     /locus_tag="VFS75_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU4677777.1"
                     /translation="SSSYHHSMYFVGYLIRGEAGAWHTGLVKDVSERYHSRKLYEKVP
                     PHLTLYRPFKTDDIEGVRSILHTFALSLPWGEVHVDGYGRFDDKVVYATALVEEKTKT
                     RVIELQKALYALPGMPENRDPWNPHATIAYRMPGEMIERIWQYVVGIPRPDFKLSFDN
                     LALFRLEGTRWQVEAVFAPAR"
BASE COUNT         4639 a         7551 c         7355 g         4588 t
ORIGIN      
        1 ctcgcggctc gagcatcatg accatctcgc cgtacgggat gacaccgagc tcggaatcga
       61 actcgaccgt cggatttcga aacacgggca ccgcgatgct tcgcgcgtac atgatggtgt
      121 tcttcgcggc gaccgcgggc tctcgtcgct ccgtatggag gatctcctcg atgctccgga
      181 aggtatgcgg tccatcgtcc gcgagttcag cctcctcttc ggagggggtt tcgtacgccg
      241 gttcggtcgt caccctggtt ccgtgccgcg ggagcgcaag gctcacgacg cgaacggaga
      301 cgtcgacgac gcggtcgaag agagtatcga tgacggagac gactgctgcc atgccatcac
      361 tatagcatcg ccgaaacgga gcgaccattg acggagcgcg gcgtactctg ccgaatcggt
      421 cttacctggc gcgcggccat atttcggccg ctttcgggta caccttcgtg agcgggtgcc
      481 ccacgcagtc atgcctcctc gcgggacagt agtcccgccc gtagtggacg agccgatggt
      541 tgaaccccca ccactcggag cgcggcatga tggagagaag atcgcgctcg atgcgaacag
      601 gatcgcgaaa atcgctaagg tcgaaccgga tggcgaaccg tcggacatgg gtgtcgacgg
      661 cgatcccctc gtggacaccg aaggcattgg agagaatgac gttcgccgtc ttccgcgcga
      721 cgccggggac gcgaaggagc tcctccatcg tccgtggcac gtccccgccg aactcgtctc
      781 gcaccatcgc cgcggcggcg cgtatgttct tcgccttatt tcggtagaaa ccgcacgaga
      841 agatatcgcg ctcaagcgct ccctgtgaga cacgggcgta gtcgtcgacc gtacgatatt
      901 ttttgaagag cttcgcggtc gtcgcgttaa cgcgtttgtc cgtgcactgc gcggagagca
      961 tgaccgcgac gagaagttcc caatcgttct catggtcgag cgctatcttc ggttcgggat
     1021 agagccgccg gaggacgcgg ttcatggcac gcacccgctt tcgacgttct tcacgctgtt
     1081 tctcggtcat cgcgcaagta taacaaaaac gcgaggacgc acgcgccccc gcgttctctg
     1141 ttcgtaacca acctatgagt tacacagcgt gcaatggccc ttgtgcgtga tgacctcgta
     1201 gatggccgag aggccgacga ggatgtacac cacctgcgtg accgttcccg tcccgaagat
     1261 aacctcgacg aggttgatat cgaacgcgac gagaagccag ttcaaacctc cgacgacgag
     1321 gaggacgaac gccacgatgt gcaacaattt gttgttcatc ttgtaggtta ctaagactta
     1381 taacgtcttc ataattatat cacccaaaga agagggcgga gcggccctgg gtataaccct
     1441 agccccggat ggcgcggtag acgagcgtca tgaacgcgcc gaaggtcacc atggcaagcg
     1501 cgaacgcggc gagtccgccg acgtacggca caagcggcaa aagagcgaag aggacgctcc
     1561 cgagaaacgc cgccggaagc gaaaatggcg gccgcttcaa gacgtggaca ccgacgtaat
     1621 gcccgacgag aacgccggcg gtgaggagcg acccgatgag cgcggtaaga tacgcgacga
     1681 aaagcgcgac gccaagaacg ccgccgatca cgctcacgat gagtatcgag gagacgaacg
     1741 gcacgaggat gagggacgcg atgccgatga gccccgactg gccgagcgac gtcgatgcga
     1801 cctcgacgac gcgagcgacc ttcgctcggc cgacgaaata gagggacatg gtcgcgaaga
     1861 cgagcatgaa cacgcccgcg aggtacgtcc gcgcgacgct cgccggaacg tccttcggct
     1921 ggacgtcgct ccggtggacc gaaccaccga cgtgcgcgtt ctgcgcacgg gtaagctcgc
     1981 tctcgctctc gtacgaaacg tcgcccgcaa tgtcggcgtt atcgccgagg gtaaggttcg
     2041 atttcgtctc gagcgatacg tccccgccca ccttcgcgtt gatgcgcgcg gtctccgcgc
     2101 ttccgtggac gctccccgcg acgggtcctt cgacggtaag gtcaccgccc atgaagatga
     2161 catccccacc gacgctcgcg gtcgagagga cggtgagctt gccgccgagg acgacgacgt
     2221 cgcccttgac cgactttccg agcgtgacgt cgccggccac tacgcggagg tcatcgccga
     2281 cgtcaccatg tacctggacg gttccgccga gagccgcgag gtcggagcct atcggcgcgt
     2341 tcactgtcac cgtccctccc gcgacgtacg cgtcgtcctt cgcgtcgccg gagacggtca
     2401 ctgacggcgc gaagccgtag aaatcaccgc ttaagacctg cgccgcgtcg atcgagatgt
     2461 tctcccctgt ccgaatgatc ggtcctgccg cgaacgccgg gagggcgaag aggagcccga
     2521 gcgcgatgat gatggttcgt ttcataggat agagtatagc gcctttacgg ccgtccgcca
     2581 cctatttctt gaggcatggg aggagcttca cggactcaag gggcgtcggc gcggcgccgc
     2641 ccatgaggtc gttcacgcgg tccgcgccga tcgcatcctc agcgcacgcg accatttcgg
     2701 gagtgatggt gatggacgcg tcgccgagcc cgaccgcgtc gagcgccttc tgctgcgtct
     2761 ttgagaggtc cgacgccttc acggtgatcg gctcggtcac ggtcggtgct tcaggcgtcc
     2821 ccgtcgcggc ggcgggcgtc gatgtcgcag tgaccccatg agtttcccta tcaagcgtct
     2881 ggacggtgta ccagacgaag accccagcgc cgatggcgat gagcagaaac gcgatggcgg
     2941 cgatgagcgc gagtttcccg atgttcatac ggagggtgcg ttagccatgg taaacgatcg
     3001 gtgattgcat gaaatacgct gcgacgagaa cggcggcgac gagcgcgatg aggatgagga
     3061 gcgcgtagag ccgaggccgc gagagctccc actcgccgtc gtaccaatta cggacgaaga
     3121 ggaacggaag gatgaagcgg atgatgtcca tacgttcagt atacgctacg tcgagctcga
     3181 gaaaagctgc ttccctcgtt cccacatctc cgggtggccc tgctttttga gccagtacca
     3241 ccattcgccg ccccagagga actgcgtgtc gtagcgcgta cccttcgcgt agtcgatgat
     3301 ctcatcgaac ttctcgagat ccatgcgcga gaattgcgtc tcgagaggcg catcgacgac
     3361 cggggtgaga agccagggct cgagctcgag ctcgatgagg aaggtcttct tctccccgta
     3421 gaggagcgcc atgaccttct ccttcgcacg gtagaaccac ggcgggagga ccgtcttgaa
     3481 ctgaccgagt tgcggattcc aaaagtatac gtagacgctc gtaccgaacg catcgccgtt
     3541 ctcgtacgcg cctcgccacg tgccgagatt tccgctatcg gtcacgagga caggccgcgt
     3601 cgggtcgagc gtgtgaacga gcgctatctc ctctttgagg aaatcctcat cgaggtcgcc
     3661 gcagtactcg ttcgcgaaga gattgaggta tggctcgttc tcgacctgcc agtacgtgat
     3721 cgcggacgag tccttatagt ggttcacgac ggtcgtgagg taatcccgta tctccgcctt
     3781 ctgctcgtcc cacgtcatgt ccttcgccca gtcggggatg tgacactccg gccagcgcgg
     3841 taaccggcgt cccaccgcga agatgacgtg ggcgtcggcc gcctcggcct tctcgatgtc
     3901 gcgatccatc catgtgaaat cgtactgccc tcgcttcggc tcgatgaggg gccagtgcgc
     3961 ggcgagcctg aagttccgga cgcccatatc gtctatgagc gcgtcgaaga cgtcgtcggg
     4021 gtcgagaccg agttccttcg cgtacgggac gctaaacgac atgccgtagg tgatggtcgt
     4081 cggtgcctcc tttcgtgcga gcgcgaacag gacgagcccg acgacgccga gtaagaggac
     4141 gagaccgatg agtaccttga ggagccgttt gcgcatcacg tgaataggac tagaaagccg
     4201 agcgatatga tggcgacgaa gagggtcttc tggaggatcg tgtcgtgacg gtacatctcg
     4261 ccgacgtggc cgcgacgcgg cttcagcgtg aagaagatgc cgatgaagag gatgaacacg
     4321 aactggaggc caccgagcgc ctggacgacc gcgacgtcgc cgagctcggt cgccttcaag
     4381 atgaggatgg tcgcgacgcc cgcgagcacc ttattcgcga ggatgagcgt gccggtcgca
     4441 cgagtcacct tcttcgtgtg tactttgatc ttgtcgtagt acgcaggaac gaggagaagc
     4501 gtgagcgcga agagcatgaa cgcgatcctc gaccagaaaa aaccgtcgtc gaacgacgtc
     4561 acgttgaaga gtcccttcat cgagacgtag tgaaacgcga agaagagtcc cgagtggatg
     4621 gaggtgagcg ccgtcttcca cccgaaatgg tagcgcgaga caaagtacgt gccgatcgag
     4681 aggagcgtga tcccgaggag gaagttcgag gatatcgcac cgtgaaggaa gaagtacccg
     4741 aggccgagtg agccgatcgc cgaggtcgcg ccgacgacgg gaacgacgtc ggccgcgtcc
     4801 gcgcagcgga acgccgtgaa catggagacg aggccgatga agaagcagta cgccgcgagg
     4861 aacgacagtg cgacgacctc gagcgttggg cggtagacgt tctcgaagga cgggatgtgg
     4921 aatccgccga tgacgggcac gaaactcacg aggtagacga gcacccacgc gcccgagatg
     4981 aggagcgtgt agtacgcgta cacgaacgga cgcgggagta tcttgtcgtc ggagacgatg
     5041 cgcttgtcga cgacggcgac gagcgcattg atgaattgcg cgccggcggc gagcagtatc
     5101 cagctcatgg ctttattcta ccgcattccg ctcacagatt tccttataga cgtacgccga
     5161 aggtcggata actctcgtga gggtcttgta gtctatctcg acgagcccga agcgcttttc
     5221 gaagccgagc gcccactcgt agttgtcgag taaggaccag tacatgtgcc cgcgaacgtc
     5281 gacgccgcgc tggatcgcgc gagagaccgc ccggacctgt ctcgtgatgt agtccgctcg
     5341 tctatcgtcc ttcgcgtcgg cgatgccgga ctccgcgacg tagagcgggc gcttgtaacg
     5401 cgcgagcatg caaagcgcgg cctcgatgcc ctcggggtag atgtcccagt ccatgtcggt
     5461 cttcgcgtac gtcgcagtgt cgccgaactt cctatggaaa tagtagttga ggcctatcga
     5521 atcgcactta cggtacacgc gacgcatgaa gcggtgcgtc caaaagcggt tcgcgaccca
     5581 cgcgacgagc ttgttcacgg gattccagtt cgcatggaag agaatgacgt gcttcacgac
     5641 gcttacgtcg acgtgccgcg cgaccttctt gatctcgtcg tacgcgcggt tgtgcgcgcg
     5701 agcgaggttc cgcatgacgc ggaaatagag gaagagcggt ttgaggccgc gtgcggcctt
     5761 cgattcgaac gttcgacccg agttcgtgat cgacacgagg tcggtgagcg agaagcgctt
     5821 aaagggcggc cagcttccgc gaagccatcc gttcgaaccg taaacgttcg gttcgttcat
     5881 cgtagaaaag tcgccgcaga agtccccgag ctgcgagacg acgtacgcac agtaccgggc
     5941 gaacacttcc ggcgaatcca tgcgctcgaa tccgcccgat tccgagaacc acaatgggag
     6001 cgtgaagtgc cagagcgtga tgtacggctt catgccgcgg accgtaagcg ccgtgaggac
     6061 ctttcggtag tgtgctatcg cctcctcgtt ccatttcccc tcctccggtt cgatgcgcgc
     6121 ccactcgatc gaaagccgat gcgcgttgtg gccgagcgac ttcgcgatgt cgaagtcctc
     6181 ctcataccgg tggtagtggt cgcacgcgat gccacaggac ggaacccgcc cttctcgggc
     6241 cgccttcgcc caatcggtgt tctcgatgcc cccctcgacc tggtacgacg cggtcgcggc
     6301 gccccagtag aaccctcgcg gaaattcctt cacgttgttc gtttcgttca ttacggacag
     6361 tgtaccaaag aaaaggcccg tccatagcga actatgaacg ggccgaacga tgactcaaag
     6421 gagcgggacg gtacggaagc ggcttccgtc gccgagccga ccttcaccgt ctttcacgac
     6481 ggcgacctgt gcacccgcgt ggtcgacacc gaagaacgtg acgtcaccgg gtccgatgag
     6541 gagccgcact tgaccgcgaa cctcgatgcg cgagaccccg atcacgtcgt cggtgatcca
     6601 ccccacgtgc cacggatact ccggcgcgac gggacggagg acgatgtagt ccttccatcc
     6661 gacgaccggc atgtggaaca ccggaagctt tccccaccac cgcgcgcgac tctgccgtcc
     6721 cttttcatgg accatgacga acgggtcgag gtgcgtgatg tcgctcgaac ggagcttcgt
     6781 gttgttccag cgatgcgtcc gctgcggcga ttcgccgacg tggcccgaga ggagcaccat
     6841 gagcggtgcc atgagccgat ccgccgcctc tgcgaccggt ccgaggggaa cgatgcgtat
     6901 cgtatccacc taacatctcc tgtgaattga cctgtataaa attataacac gatttttcta
     6961 atttttcaat tcctcacgaa aggcccgcgg tgcgagcgtt tcgagccact tctcgtaaag
     7021 cgcggtcgtc gcgacgacgt cgcggctatt atactcggcg atgtcgcgaa agcgcttctc
     7081 cttgaagaga ccggcgacgt cgtcgccagc gaccccctcc cccttcgggc tctcgatgcc
     7141 gaacgcgtga cagaagaggt ggagagacgg ccgcttgtgc atcgctccgt agaacgtgag
     7201 ctggtcctgg aggtcgacgt gccgcgcgta cttctgttgg tagaggtagc ggccgtccat
     7261 gaggtccttc gaagggcgaa tgccatgaac cgacgaacga aggttcataa acggaacgtc
     7321 gaacccgcga ccgttgaacg tgacgaacgt atcgtagctc ttcgcgccgt cccagaaggc
     7381 ctcgagcata tcgcgctctg agcggggttt tagcgcgaat tccccggtcc ttccctctcc
     7441 ttccccctcc tccgcctcgt agtacacgac accctgctta cgctcgaggt catagagacc
     7501 gatcgcgacg atgcggccgg tgagtggcga gaagccgagt ccttcgcgca cgtccttgag
     7561 acccgcctcg agctcgtcct cgcttcgcgc cgttcgctcg acccaacgcg tgagcatcga
     7621 ctgcgtcgtc tcgtcgagcg agttccactc ctcgccgacg gtttcgatat cgaaaatgag
     7681 agtcttcatg gtgcgcggtc agagcgcctc tgtcgtcgtg ccgggtgtct ccgtcgccga
     7741 ggctgggagg agaggaagcg gttcgggagt cggcagtggc gcgggagcgg ggctcacgac
     7801 cggacgcgag agttcgtcgc ggcgaagcgc tgcccgcacc ttcgcgatga acgcgtcacg
     7861 ccattcgctc gacggttcgt tcgtgacgac cgtcgccgcg taggacgctt tcggcgtcgc
     7921 gaagatggcg ctcgcgcggt tctcgtccgc tttgacctcc gggttcggga acgcgagcgc
     7981 cgacgagagc gcgagcgtcg cgacgacgag aatgagaaag tgcttcgtcc gtatcatacc
     8041 cttatagtac caaacgccgc cgaaacggtc taaacgatct gcacatcgat gagcttctcg
     8101 gcgcgccgaa tgtccgcgag gagcttcgcg cgctcgtcat tatagagcgt gagcgtgaac
     8161 accgcctttc ccccgttctt cgcgaacttg cagaggtcgc ccaccttctt gaggaccgat
     8221 atctccgcga acgacttgag ctcctggcac ttcttgatgc cctttagctt cgtgatgcgc
     8281 ccctccttca tcggatacca cttgagggtc gccgcgaacc ccttgcactt cttcggcagg
     8341 atgggccgct tgggaaggcg gatgagtatg tcgttcatcg agtggtcgat gtcgcaggag
     8401 agctcgtgaa gcttgtgacg gaacccgccg acgcgcggcc cgatctcgat gagcttccac
     8461 tcgtcgtcga gtttcatgag ttcgacatgc gcggtcgtgt ttcgaaggcc gagcgcgtgg
     8521 atacccgcgg tcgccgccat gtgcgccgct tcgacgctct tcttcttcag tcccgtcgga
     8581 gacatgtgga tgtagttcga gaaatcatcg tgtcccatgt tcttccgcgt gaagacgcgg
     8641 accatgggac aaaagtacac gcgcccgagc ccgttcacgt acgcgtcgac cgagtacatg
     8701 tcgccttcca tgaactcctc gaccatcatc ttcggcgtct cgatgcgtcc gttctccgcg
     8761 tatatcttct ctatcttctt gaaggactgc gcgaggacct ctttgagctc atcctcgtgg
     8821 tagcacacgg agacgaggag gctctgcgcg aggttcgcgg gcttcacgat catcgggaac
     8881 ttgatccgcc cgatgacgcg cctccgctcc tccttggtgt tcgcgcgaac gatcgcgtac
     8941 ttcggcgagt accgcttcgc gaggagcttg aagcgccgtc gcatctcgag cttgtcggtc
     9001 gcccagcgaa gcgattcggt cgtcggcgtc cggaggtacg gcacgtgcgg gatgacctgg
     9061 acgaaacgcg ccatgtacga ttccgctcga cacgtgatgg cgcaaagctc gttctggtac
     9121 ggcgcgagcg cctcggcgat gcgcgccggc acggagaaat cgcactcgac gaggatgtcg
     9181 agccccttgt agtcgtcacg gcccttctcc cgctgatgat ggacgtcctt gatgagcatt
     9241 acgcggaact tccgcttctc gcgtttctcg tacgcgcgaa tgctctcgat ggcgccggga
     9301 gggagcgtca tcacgtagac gatgatgttc cgttccgcct tcatagatcc atgctggcaa
     9361 taaaaaatgc caatgttgaa cttggcaatc gaattacgta tagtcatcat accacaacgg
     9421 cgaacgccgt tccataggca tgaaacagcg gctctacctc atcaacaaca aagtcggcga
     9481 cgcgtccttc cgcgacgccg tgcgccttgt cgcgcgcgag cacggtacat cggtctcgtt
     9541 cctcttcgcg aaccgattcg cgttccactg cccgcgcggc cggcgcgacg tctctctcta
     9601 ccacgacggg aaacgggtac ccttccggcg cgaggaccgc gtgttcgtgc gcgcgtgggg
     9661 cgcgcatccg accgcgaccg cgctcctctc gatagtcctt cggagcctcg gcatccgctt
     9721 cgtcgacgaa cgagtgcacg tgagtcacgg catccgaagc tcgaagctcg cccatgcctt
     9781 catcctcaag gaggccggta tcgcgagtcc gaacacatgg gtcgcgacgc cgcgctcctt
     9841 ccccaccgtc cgtgcgcgca tcgagaagga cctccgattc ccgctcgtcg tgaagacgaa
     9901 ggggagcaag ggcaaatgcg tctggaaatg cgatacccgt gcggcgctcg acgcgcgcgt
     9961 ggcgaaactc cggaagagcg agctcatcct ctttcaggag tacgtgccga acgagagcga
    10021 cattcgcgcc atcgtattcg atggcgccct catcggcggc atcgagcgca gttcgaagga
    10081 cggtttctat aacaacatcg cgaaaggcgc gagcgcgcgc ctcgttcccc tctcgaggga
    10141 ggagacgcgg atcgcgaaga aggccgcgca tgtcgtcggg ctcgacctcg ccggcgtcga
    10201 catcgtacga agcgcacacg gacccttggt gttcgaggtc aacaaggctc ctgacatcag
    10261 cggattcaac gatgccgcgg ggttcaacat cgcggaagcc atttttaggc ggtacctctc
    10321 tcgagcgcgc tcatgacgta cgtttcggcc acgcgacgat tcgtcccctc tgtacatatt
    10381 ccggtcgatc gctccgtgat agagtgcgcc ctatgaagat caaatacgca tcggcgcacc
    10441 gttggcttaa gccggtaccg aacgacgccc tcgcgtcgtt cggaaagaac tcgcgggcac
    10501 gcctgcggag cgcgctcccg aagatagcgg cgctccccgt tcgtaacgag atcgcgaagc
    10561 tcgacgaggc gttcctcgag cgattcctcc cccgctatga ggcccgcatc ggagagaagc
    10621 gtcatccggt cgtcttcgac gtacgcgcga agacgctcgg ctccgccaag gccgataggt
    10681 actacgctat gtcgctctac gagggcgacc ggttcttagg cggcaccatc ttcgccatcc
    10741 gcaaggaccg actcgccgtc gcgtaccgca cgtacgagaa cgcatggcac ggcgcacgcc
    10801 tccaggcgaa cccgtccctg tacgtcgagt actgcctcaa tatccacgcg aaggagatcg
    10861 gcgtcgcgta catcgtccac ggcaaggacc gcaatcccta cggcgtcaat tcgaacatcg
    10921 gcctcgctgc gttcaaactc gccgcgggct gccatgcgga gataccgaga aagttcgaaa
    10981 taggcgaact cgacacggaa acatgtgcgg aggatgcgct cgttctcgag tacccgggcg
    11041 acgaaccgcg agcgcccata cggcgagcgt acctcgtcac gaccgctgaa acggaggctg
    11101 cctggacgca ggtcgtgaag tacccgcacc tcctctccgt cgcgaccatc taccgaacga
    11161 aaagaccgga ggatgcgctc ctccggctcg gtacgaacgt cgcgtgaatc agtacggtat
    11221 cttgcactcg tcgaaacagt gcgcctcgcg attcagcttg tagacgcgct cgcgtcctcg
    11281 gtcctcgacg tcgacggcgt tcaccgatct caatatgttc aggtggtgcg taatggtcgg
    11341 ctgcttcagg ccgagttgtt cggtcaattt cccgacggtc tctccctttt tcgcctttcc
    11401 gagaagacag acgagcttgt agcggctctg gacaccgacg accttgaagc aatcggggca
    11461 aagttcctcc tccgtcgcga ccgacttctt cacccacgct ggacgcgggt atgtttttgt
    11521 ttccgagtga acgccgttcg ttcgtttttt ttccttttcc acagttcgat gaattgatgt
    11581 gcgtctatat agacgacgac ctatatataa tcttcgtata gattaccatc aataataaca
    11641 atatgcggtg gcatcggcaa ccgcgtttta atcaactcta tgccacagac aacacgacgt
    11701 gagcttacgc acattatcaa gcgctccggg cgcagggttc ccttcagcga ggggaagatc
    11761 gcgcgcgcga tcgagaaggc gctccgcgcg accgatgcgt atcgcgcggg cgcgccggag
    11821 aagatagcga aggcggtcat cgccgaactc cgcgaacgac acaaggcgga cgagacctac
    11881 gttccctcgg tcgaggaggt ccaggacgtc attgagatga cgctcatgga ggtaggacac
    11941 catgagacgg cgaaggcgta catcctgtac cgagaacagc acgcacagat ccggcggatg
    12001 gtatcgaccg agtcgatcga cctcgtagac cagtacctcg cgcgggccga ttggcaggtg
    12061 aacgagaaca gcaacatggc gttctcgctc caggggctca ataactacat cgcctcggag
    12121 gtgagcaaga actactggct caacaagatc taccccgcgg aggtgcgaaa agcgcacgag
    12181 gagggcgatt tccacgtgca tgacctcaac ctcctctccg tctactgcgt cggctgggac
    12241 ctctacgacc ttctccggcg cggcttcggc ggtgtcgccg ggaagataca gtcgaagccg
    12301 ccgaagcatt tccgcgcggc cctcggtcaa atggtgaact tcttctatac gctccaaggc
    12361 gaagccgcgg gcgcgcaagc gttctcgaac gtcgacaccc ttctcgcgcc gttcatctgg
    12421 tacgaccacc tctcgtacga cgaggtgaag caggcgctcc aggagttcgt ctttaacgtg
    12481 aacgtgccga cgcgcgtcgg tttccagacg ccgttcacga acatcacgct cgacctcacc
    12541 gtcccctcgt tctacgagaa attgcgcgtc atcgtcggcg gaaaggagca gaaggagacg
    12601 tacggggagt tcacgcgcga gatggacatg ttcaatcgag cgctcctcga ggtcatggtg
    12661 gcgggcgacg cctctggccg cgtcttcacg ttccccatcc cgacgtacaa catcacgaag
    12721 gacttcgact gggagaacga gaccatcgtc ggcctctggg aggcggcggc gaagtacggc
    12781 atcccctact tctcgaactt cgtgaactcg gacatgagcc cggacgacgc gcgctcgatg
    12841 tgttgccgac ttcgcatcga caatcgcgag ctcatgaagc gcggcggcgg tctcttcggc
    12901 gcgaacccgc tcaccggctc gatcggcgtc gtcacgatga accttccgcg cctcggaaag
    12961 acgtcgaagg acgaggccga gttcctcgag aaactcggcc gcctcatgga cgtcgcgaag
    13021 gagagcctcg agataaaacg aaaggtcctc gagtccatga ccgacaagaa cctctatccg
    13081 tacacgaagt actacctcga caccgtgaag accgggttcg gcgagtactg gaagaaccac
    13141 ttctcgacga tcggcctcgt cggtatgaac gaggcgatac gaaacctcct cgggacctcc
    13201 atcggtacaa aggagggcat cgcgttctcg gagcgcgtcc tcgactatat gcgaaaccgt
    13261 atcgccgact accaggagga gacgggcaac aactacaatc tcgaggcgac gcccgccgag
    13321 gggacggcgt accggctcgc gctctccgac caaaagcggt tcggcgacat gattttcgcg
    13381 aacggtacgg gcgacgaggt cgcggacccg tactacacga actcgacgca ccttcccgtc
    13441 cagttcaccg acgacatgtt cgagatactc gaacttcagg acacgctcca gacgaagtac
    13501 acgggcggca cggtcatcca cttcttcctc ggcgagcgcg tgcgggacgc atcggtcgtg
    13561 aagaagctcg tccgaaagat ctgcgagacg taccacctcc cctacttcac catcacgccg
    13621 accttcagcg tctgtaagaa ccatgggtat ctccacggcg aggtcttcga gtgtccgacg
    13681 tgtcacgagc cctgcgaggt ctatacgcgt atcgtcgggt acctgcggcc ggtcgatcag
    13741 tggaacaaag gcaagaaggc cgagtacgcg aaccggaccg agatcgagtc gcgcgcgctc
    13801 gaggcgaagg aggccgcgct tgcggtatga ggatcggcgg tcttcagcac tcgtccctca
    13861 tcgactaccc cgggcatatc ggcgcgatcg tcttcaccgt cggctgtaac ttccgctgtc
    13921 cgtactgcca taacccggaa ctcgtcgacg agacggcgga tgagctcacg gaggaacatg
    13981 tcctcgagtt cctcgaaaag cgccgcgggc tcctcgaggc ggtgacgata accggcggcg
    14041 agccgacgat gcacgcggac cttcccgact ttcttcgaaa ggtcaaaggg ctcgggttcc
    14101 tcgtcaaact cgactcgaac gggacgaacc ccgatatgct cgagcgggtg atcggagacg
    14161 ggttcgtcga ctacgtcgcg atggacgtga agtcaccgct ctcgcgctac gaggcgaccg
    14221 tcgggcgccc ggtcaacact gaggcgctta gacgctcgat cgcgctcctc atcgcgggcc
    14281 gcatcgggta cgagttccgc acgaccgtcg tgaagtcgct cctctcaaag gaagacgtcc
    14341 gggggatcgg cgaggcgatc gaaggggcgc gcctccacta cctccaaaag ttcgtctcgc
    14401 aaaagacgct caatcccgcc ttcctcaaga agacgaccta cagcgacgag gagttcgagg
    14461 agatgcagaa ggtcatggag gagtacgtcg acacgtgtac gatccgctaa ccatatgaga
    14521 accatctacc tcgatcatgc cgcatcgacg ccgcttcgca cggaggtcct ccaggcgatg
    14581 ctcccgtacc tcgaaggcgc gtacgggaac ccttcgtcgc tccaccacaa ggggcgcgag
    14641 gccgagcgtg cgctccgcca cgcgagagcg aaggtcgccg cgctcctcgc ggtcgctcct
    14701 gacgaggtcg tcttcaccgg gtcggggacc gaggcgaaca atctcgccct cctcggcacc
    14761 gcagaagcac gctcctcgcg cgggcgacac gtgctcgttt cgccgatcga gcatccctca
    14821 gtcctctctc cggcggaagc gctcgcgcgg gcgggtttcg atgtcgaata tctccccgtc
    14881 gacgcgtatg ggcgcatcga cgcgggagat gcgtacggac gcgttcgacc cgacaccatc
    14941 ctcgtctccg tcatgtacgc gaacaacgag atcggtaccg tagagccgat cgcagagctc
    15001 ggagagctcc tcgccgcgct ccccgagacc gctcggccgc tcctccatac ggacgcctgt
    15061 caggctctcg gccagcttcc gatatcgccc gtagaccttc acgtcgacct catgagcctc
    15121 aacagcgcga aggcgtacgg cccgaagggc gtcggtatgc tctacgtgcg tacgggcgtc
    15181 cgcctcgcgc cgcgtcaact cggcggaagc catgagcgcg gcctccgcgc cggcaccgag
    15241 aatctcgcgg gcatcgtcgg cttttcgtcg gcgctcgagc tcgcgatccc acgcgcgcac
    15301 gagaccgcgc gccggctctc gtcgctccga aacgagttca tcggcatggt gcgccgactc
    15361 gtgccgagtg cgaccgtaaa cggccacccg acggagcgcc ttcccaacaa cgtccacctc
    15421 tccgtaccgt acatcgaagg cgaatcgctc cttctccttc tcgatacgta cggcatctgc
    15481 gccgcgaccg gctctgcctg tagcgcccac gacctcgctc cctcccatgt ccttcgcgcc
    15541 atcggacaat ccgacgagct catccacgga agcctccgcc tttccttcgg cgacgagacg
    15601 acccgcgacg acctcgcgta cgtcgcggag gcgcttgccg cctcggtccg cagacttacc
    15661 agcgtatcac ctctccccct acacgtatga aaacgaatct cgagaacacc gccgacgtcg
    15721 cgtcgtgcga ctccggcgag tcatggctct acagtgacgt agtcaaagac cacttcttcc
    15781 atccgcgaaa cattctcctc gacgacgggt acgagtgcga cggcgtgggc gtcgtcggca
    15841 gtccggcgtg cggcgatatg atggtcgtct ggatacgggt cgatgcaggg accggccgca
    15901 tcagcgagtg caagtggcgc acgttcgggt gcgcgtcggc gatcgcgtcg acatcgatga
    15961 tgtccgtcat ggcgaccgag gagggcggca tgacgctccg cgacgcgaaa cgtctcatgc
    16021 ccgaagccat tctcgaacga ctcggcggcc ttcccgacag gaagtaccac tgctccgtcc
    16081 tcgggcacca tgcgctccga gaggccgtcc tcgactacga ggagaagcga aacgagatat
    16141 gagcgcgaac gaaccggtcg aacgtacgtg ggtcgtgaga gatgcgcgag acgaggcgcc
    16201 gggcgttcgc tcgctgtacc tccaggctgt gggagaacgt cctcggttca tcgcggggca
    16261 gtacctcacc gtacgcctcc ccggctatga acccgttgag ggcaagtcgt acagcatatc
    16321 gagcgccgag gacgatgcgc aggtccggct cacggtgaag gaggccggcc ccttctcgcg
    16381 cgccatcctc gcgaaacgac ccggcgatct tctgcgcacg tccgcgccgt acgggttctt
    16441 ctaccccgag cgagatgcca tgtcagacct cgtgttcatc gcgggcggta tcggggtgac
    16501 gcccgcgatg ggcatcatcg agacgctcct taacgatggc tatgcgggct cggtgacgct
    16561 cttctattcc aacaagacgg aagcggacat cgcctttcgc gcgcgcctcg agtcccttgc
    16621 ggcgatgcac gaacgcttca cgctccgaaa taacgtcacg cgagagcacc cgcgcgatcc
    16681 atcctacgaa gtgggtcgac tctccggttc gtccatcgtt cgttcgctct ccggttcgcc
    16741 tactgcggca gactatttcg tctcgggttc catcgacttc acgcggacgc tctggaaaga
    16801 gctgcggcat gagggagtgc cgcagtcttc catatacaca gaggggttct actgacgctt
    16861 agcgatcggt cgtgaacgag cggacggaac cgcactcgat gcggtcgtcg ccgtcgttct
    16921 cgtacgcgac gcagatccga tggaagatgt cgcggtcgtt gtcgagcccg ctcacccgct
    16981 ttgtgtacga ctcctcgccg tcgagatcgc tgtcgacgct caccttctgt aggtcgctcc
    17041 cctcctcgtc gatgtcgctg taccgatcat agtccgcgac gtcgtcgacg aggcgctcgt
    17101 cctcgccgta gacgaagaac accgtgccgc tgtcgaaatc gcgcatatcg accgtgccgc
    17161 ggagcaccgc ggaatcgtcc gtcacgtcgg tcgcgctctg tgtcgtcacg tcgggagtgt
    17221 cctcggatac cgtccgcccc gtatcgaatg acaggaacga gcctcggtcg atgtcgccgt
    17281 tgtcatcgcg cgcgacggcg cggaagcggt agctcgtgtt ctgggcgagc cctcctacgg
    17341 tgcggctgaa cgactcgttg tcattgtcgt cgatgcgctc gctcgacgtc cgcatgagcg
    17401 cagaaccgtt atccttgccg aactcgaacc acacgtcggc gtagttcgaa ctgccgaagt
    17461 cgtcgatgcg gccgcgaaga agcgcgctgt acggcatgac gttcgtcgcg cttacggtcg
    17521 cgacgtcgac ctcggacgtg ccgctcgtct ggccctgcag ttcgttcagg tactggataa
    17581 gctcctcgag gaacgcatag agctgcgagt agttcgtgat gtaccggtct tggttcctga
    17641 accgatacat caggttgaat tgcgtctggg tttgcgccgc gctcgcatgc gatgcgctta
    17701 tcggcgtgta gacgaccgcg gcggccacta tgaaagccgc gacgcccagc gttaatgcct
    17761 ttttcatgtt ggttgctgtt aaatgagtaa agcctctaac agccccatgg tgatcacatt
    17821 gtacacctgc tagaaaattc gcgtcaatga aaatcggccc gctctagaat tcaccgggcg
    17881 cgggaacgaa cgtgatggaa tcgaggagcg attcataatc gcggtagata ggcgatgcct
    17941 cgtcgaggta ctcaccacga acgacgtata tgcgtgaccc atgcgcgatg accgtcacgc
    18001 tcgcgcggta gaggccgtcc gccgcgtagc ggatcgcgtt cgcgccgccg acgacgagat
    18061 cttcggcgtc gccgattttc tgtccgtagc cgctttgagc ggtatgcgat tcggcccatg
    18121 cgcccggaaa ctgtttgctc gcattgtcgt agacgacgat cgagagcgtc ggcggaagtt
    18181 caccgtctgc ggccggcgta tcgtacaccg ccgcgtccgc ggcatggacg aggacgatgg
    18241 tcttctccgc ctccctctct gcgatgcgcg ggtctcgtac gacgtacccc gcgtcgccgg
    18301 atgggtacgt gaaggagaga ccgagcgatt cgtcgcggta gagcgtgagc gcgtcgttct
    18361 cgagcggtac cggcgactcg acgccctcgg gagcagagag gggcgcgttc gccttgggcc
    18421 gcgtgaggaa gtacgcgacg aagataacgg cgacgatgag cgtgatggtg agaagatggc
    18481 gcatagcgag attgtatcac gagacatcgt ctgggtcgta cacgaaacgc caaggcttcg
    18541 ccgcccagtg ttcgccggcg tatgcgacgc cgatgcgcgg cgtgttccgt atacggcgcg
    18601 acggcacgcg ggtgcctcgg tcttcgatcc agagccccgt ctccttcgtg gccctgttcg
    18661 cgttctctgt cttcgtgacg gcgagcgctc gcgtgagtcg accggggccc gaaaccatgt
    18721 ccgtcccgcg gatgagcacg gcggatgggt ggccgttcgg tccggtcacg atattgagca
    18781 tcgagtacat gccgtagatg aggtatacat accatcgccc cgactccccg aacatgacct
    18841 cggtgtgcgg cgtccgtcct ctcgacgcgt gcgaggcgag gtcgtccagc ccctcgtacg
    18901 cctcggtctc cgtgatcatc cctgcgagct cacggccgcg tacgcgccgc acgagaaatt
    18961 tcccgaggag ctctcgggcg acgaccggag ctgggcgcga gtagaacgat gggccgagga
    19021 cctttcgcat gctatgtgag gtacttcttg cccttcaatt tctccgggaa aagatcttcc
    19081 ttcgtatatc gttcataccc cttcatatac ccgaggtcct tcatgagttt cgtcggagcg
    19141 ttccggagct tgagcggcac cgggagattg ccgtacgcct cggcgtcctt catcgcgcgc
    19201 atgtacgcat cgtacgcgcg gcggtccttc ttcgcctctg agaggtacac gacgccatgg
    19261 gcgagattga tgccacactc gggaagtcct atcgtttcga ccgcgcggaa gacgtcgttc
    19321 gcgacgacga gagcggtcgg ctgcgcgaga ccgatgtctt cgctcgcgaa gacgaccatc
    19381 ctccgcgcga tgaacttggg gtcctcgccc gcctgtatca tccgcgcgag gtagtagaga
    19441 gccgcgtccg gctgtgaggc ccgcatgctc ttgatgaacg cgcttatggt gttgtagtgc
    19501 tcctcccctt tcttgtcgta ccggaggaat ttcgactgga gcgcctcggt gaggttctcg
    19561 accgtgagtt ttccgtagag gcggagcgtc gcctcgatga ccgcgatggc ctgccgcgcg
    19621 tcgccgtttg ccatgccaat gagccactcg cgagctggct ttcccatctt caccttctcc
    19681 ttcttcgcga cccggacgat tatcttctcc atgtcctccg gcgagagctc gttgaggacg
    19741 aagacgcggc agcgcgacag caagggcgag atgacctcga agctcgggtt ctccgtcgtc
    19801 gccccgatca aggtgagcgc accggactcg acgtatggga ggagaaagtc ctgctggctc
    19861 ttattgaaac ggtgtatctc gtcgagaaag agtacggtcg ggtggagcga catcgtccgc
    19921 tctttcccct cgacgagctt ccggatgtca tccttcccgg ccgataccgc cgatagttcc
    19981 tgaaagtcac cgcctaaagc gtcggtgtag aggcgcgcga gcgtcgtttt tccgacaccg
    20041 ggcgggcccc agaggatgaa cgagaagaga tgcttcttct cgatcgcgac gcgaagcggc
    20101 ttcccgggcc ccacgaggtg ctcctgcccg acgaactcgt cgagcgtctt cggacgaatg
    20161 gcggaggcga ggggttccat agggccattg tactcttccg cctaaagtcc gctacctacg
    20221 cggctcggag cgtctcgatg tcgatcttct tcatcgaaag aagcatcgcc atgcggcgat
    20281 cgacgacggc tgtatcgtcg tcgccgagga tctcaccgag gttcgcgggc actatctgcc
    20341 aggatacgcc gtaccggtct ttgagccacc cacactgctc cgcctcggga tgcgcggaga
    20401 gcgcgctcca aagccggtcg atctccgctt ggtcctcgca tcggacgagg aacgagagcg
    20461 cctcattgaa gttgaactga tgcgggtgcg cgctatccat cgcggagaac gtctggtcct
    20521 cgagcacgaa gatgccgtgc gtcaccgaac cctcgacgtc aggttccgct cccgctccgt
    20581 agcgcgcgat catgcctatc tcggaccgcg ggaacgtctt cgtatagaaa cccatcgctt
    20641 cctcggcgcg gcccgcgacg tcacccgtga acatgagcga ggggaacata cggtgactct
    20701 cggcgttcgc gtggtaaatg agctgccacg tgagaccgta gcggtcgcga agccagccgt
    20761 accgcggact gaacgggtac gtgtcgaggt ccatgagggc cgtacccccg tcgatgaggc
    20821 tcccccagag ccggtcgacc tcctcggcgc tctcgcactc gacggtgatg gagatcgcgg
    20881 gcgtgaagtt gaagaccgga ccgccgttga gcgcgagcat ctcgaaccct cgaaccgtga
    20941 agtcgaccgt catcacggaa cctgcctgtc tcccgtgaat ctcctgcccg gcgttcgggt
    21001 agagcgttac gctcttcacc tccccgccga gaacggacac gtagaactcc gcggcctcct
    21061 tcgcctccga gtcgaaccaa agatgtggga ctattttttg catacgaaca tcgtagcatg
    21121 gcggccgcga gccgccatgc tacggcaatt tgagctggcg tttaagggtc cgggcgtcga
    21181 cgatagcgtg cccgtgcaca tcgttgttga agtacacgaa gacgtcgaaa ccgcccgacg
    21241 accaccgtgc gatgcgctcg gcccatggtt taagccgtcg cgggccgtac cgcgacgacg
    21301 cgaacgacgt cggaccatga aaacgcacgt agatgaagtc cgcggtgatg ttctcgatgg
    21361 tcggctcgaa cgacgggatc ttcgcggagt ccgggaaaac gagcgctatg ttccggtccc
    21421 agaggagcgg tcgaacgtcc gggtcgagga gacagttcat ccagctcttg tggcgcggct
    21481 cgagcgcgac gcgcacgcgg aagttccgct ccgtgacgac gaagaggtcc tggaggaacc
    21541 gcccgagcac cttcttgtcg tacgcgaggt tcggagggag ctgtacgagg acgacgccga
    21601 gcttcgtacg aagcggctcc gcgtgccgca tgaagcgttc gagggcgacg cgtgcgctct
    21661 tgatgcgctt ctcgtgcgtg atgtaccgcg agagtttcac ggagaagacg aaatgctccg
    21721 gcgtctcgcg acaccatttt tcgaaggtgc tcttcgcggg caggtggtag aaggacgagt
    21781 tcacttcgac ggtatcgaac gtccgcgcga ggtacgcgag gtggccgcgc ttcatatccg
    21841 tgggatagaa cgattcgccc cagtccttat acatccaacc cgacgtccct atgtgtatct
    21901 ttcccgggcc gtgcgccatg ctcatagtat aagcagagaa tcttaagaac ccatgaacgc
    21961 cgccgtcttc ataaaatttt aatcaccgac catatattgt agtaggacat tatcaggtta
    22021 acggatatga agcgtatgaa gtacattttc cctatcgcgc tcatcggcat gttcccgctt
    22081 ctctcgtccg cggcgacgta ccactacgtc gaccagtcgg gcgtcgtgcg cgacgtcgag
    22141 gcgccgaacg cacagacggc gatcatgatc gctcccgacc gtgacccgca cagcggcgtc
    22201 gcgatcgacg agggcgacat cgaaccgggc atggaggtcg cctctgtcgc ggggagcgac
    22261 gcgggcggct cgaacgggaa cggctcggat aacgggtcgt tgaacggcac cggcgggccg
    22321 aacacgtggc actacgtgac cgacgcgggc ctcaccgcga cggtcgaggc gccgagcccc
    22381 gaggtcgcgc tcatgcgagc gacgaacatc gacccgcaca gcggcgtcgc gatcgacgag
    22441 ggtatgatcg aggacggcga gttcgttccg gcggcggcac acaactagcc tcgtacgtaa
    22501 aaacgaccgg cctttggccg gcgtttttat atccgcatta ccagtctcag agcaccatat
    22561 gtactattcg aacttccaag cgacgctaaa cgagcttgcg gcgagccttc ccaatatcgc
    22621 cggagcggtc cttatcatcc tcatcggctg ggtcatcgcc gtcatcgtcg ccgctatcgt
    22681 gcggcgcggg ctccaatgga tgggcgccga tggccttttc gagaagaccg gcgtcaatcg
    22741 ctcgcccatc tcgtcgcgca cgcgcgtgtc gaacgtcgtc ggggccttcg tgaaatggct
    22801 catcatcctc ggggcagtcg gcatcgctgc cgagatagtg catctcgacg gcgtcgcggc
    22861 gttcatcgga tcgctcttcg cgtacgtacc gaacatcctc gtcgccgcgg tcatcctcgt
    22921 cgtcggtttc atcaccgccg acaaggtctc cgcgctcatc ggcgcgagca cgacgttctc
    22981 atcgctctcg aaggacgggc ggatgctcgt cacgcaagcg gcgaagtacg gcatcatcgt
    23041 cttctccgtc atggccgcgc tcacgcagct caatatcgtt ccccagctca tcgagattgc
    23101 cttcgcgggc ctcgtcttcg cgctcgcgct cgcgttcggc ctcggtggcc gcgagcacgc
    23161 gagcgagctc atcgcacgcc tcaagacaaa gtcgaagacg ggttagggtc gaagaggacc
    23221 tcggcgaagc agacgatgcg gtctcgcgcg tcgagctcga tgccttcggc gcggtacttc
    23281 cgcatccgct ctctgcgttc acggccgtca cttgcccaaa tgcggccgtc agcgtacacg
    23341 atacgatgga acggaatgtc gcgcgtaccg ttatcgtagg cacgcccgag tatcgaagtg
    23401 acgctctgcg cggccatcgc ccctccgccg gcgtagcgtg cgatgagccc gtacgtcatg
    23461 acgcgccctt tcggtatcgc ccgcgctatc tcgacgacgc gctcgctgaa cgtcttctcc
    23521 gatatacccc cctcgacccg tttgcgtacc atatccgtat gtcgaatgag tataccaaag
    23581 caataagcta tctggccgga gcgaaaacgg cttcgacttg ccagcgcgta ccctcgaggc
    23641 ggaaaagcgc gagattatcg aaagacagct tgaagtccgg ccgcgggatg cccacgacgt
    23701 actgccatat cctctctatc atctctcccg gcatacggta tgcgatggtc gcgtgcgggt
    23761 tccaagggtc gcgattctcc ggcataccgg ggagcgcgta gagcgccttc tggagctcga
    23821 tgacccgtgt cttcgtcttc tcttcgacga gcgcggtcgc gtagacgacc ttgtcgtcga
    23881 agcgaccgta cccgtcgaca tggacctctc cccacgggag cgaaagcgcg aacgtgtgga
    23941 ggatgctccg cacgccttcg atgtcgtccg tcttgaaggg ccggtagagc gtaaggtgcg
    24001 gcggcacctt ctcgtagagt ttccgcgaat ggtaccgctc ggacacgtcc ttcacgaggc
    24061 cggtgtgcca tgctcccgcc tccccgcgta tgaggtaccc gacgaaatac atagagtgat
    24121 gatacgacga cga
//
LOCUS       DASTSP010000077        21562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_625662, whole genome shotgun sequence.
ACCESSION   DASTSP010000077 DASTSP010000000
VERSION     DASTSP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 21562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21562
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625662"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1606
                     /locus_tag="VFS75_03630"
     CDS             86..1606
                     /locus_tag="VFS75_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEU4677778.1"
                     /translation="MQVLRVHSIRRAFTYTLAALVFFILPGVIHAATVNISPSTGSYG
                     VGQSFTVNVQVDPEGKSANAAQAQLTFDKTKLSVVNITKTQTFPFWTTEPKFSNTDGT
                     IDFGGGGPTPFSSKLTVVTINFKAIAEGSAQVSVSSAEVTAADGLGTDLYSGPGNGTF
                     TITAASTKPAENPTPTTSETPTDGGSDSTDGPITFGDPPRAPEVGSTIFMDPETWYAT
                     KVGTFTWSVPFDVDSMALDISTSSEAEAVTVMDPPIDSIALTEENLIDGVQYLTMKYK
                     NQVDWGATLHRKIMIDTIPPEPFKVNVNAGNSPSKFPLLTFEAHDEPSGIQKYQMAIA
                     GGEPFDITPDEAKLGYLLKDLEDGTYTVKVVAFDYAGNKTEATAAILITAGWHKPVET
                     VITTSIWDFFKGKNLVIILLLLALIAQFVLAHYARKQHELREQKLRKETKEIQDQMEK
                     IFSALRDEIYDQINAITKNERLSKKEKEAVEGLNQALEVSETLIEKEITDVSKILK"
     gene            1901..18736
                     /locus_tag="VFS75_03635"
     CDS             1901..18736
                     /locus_tag="VFS75_03635"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HEU4677779.1"
                     /translation="MALAVFAFTITSVAESNLSPVRKLDTLNVLPTSVESTSWHRPEE
                     ALVQDVDESGVYQSFTKTNSSYYDGKDPKDDVLPQPTIGTPSTDAHTDASSTSAESDV
                     DTSDDAPAGDSTETSTPVSEDDAAMDASTGTEEHQASEPSTDASAPSDSPSQSEAPEP
                     APSEPVTVSAPAAKSDSGASSAESPLQALSRSFFRITDVALSLLPFADVSTSAPVTTV
                     DGSSVPESAGSPTAETSTPESVDAPDMDETPVSEEPANADAGAPEGTPISEHASSDGA
                     NETATSSEETAGEPAVTTPSALRATSTDTGITFEHFELPPLEPGQLIRNVQLRMSLAG
                     QVDLPEGVAAPSLEVEYDNGSGWTSAGAVLLDGEVSNALNDGYFLFALPRFLSVDSLE
                     DFKVRVTYRGDQEALKRVFVDSVWLQVDTETFNREVLKERLLPSELAHLTHPSMYEFL
                     GTDVDFARGDVPRFSLKYESQRNAAIRFIRNLFGRDLASLQSVVVRKHSGEPIDVHPD
                     VNVTADGLISLQLSPEDTASLEPGAYSIELSVDEGGKVFTDSFEFQYGLVAINPDQTE
                     YALGATTTIMMGAVSEGGNTLCGADLHLYVIDPAGAVVEVPVTASAACDGNNLVDAPD
                     YEATYVASAPGTYEMYAERIGADGEVMAHADDTFKVVAKQGFTLRRDGPTRINPKYDY
                     PMTFTLTTAKAFTGELVERVPKDFAIVSTDASVRDAGEYQELRFAVSQKAGETMTYAY
                     RFDAPDISPYLYNLGPATLVDDANIVVVRETLDGESAFAEESVTTGTSTVDEASTATT
                     DTTGVMEVSPTEPESDASTADTSPEPDSAAPDTTSETPTPAVDAASEPTTPATSTPTD
                     APASDAVAPASPAAIPLPSAPVATDTATSTDIVTSPTNATSTVSTATSTGEKASFAEH
                     RAWQIASDATGSMIVLWTSGASIPAGWTCISCAAGNTFFQRFPVGSSTYGNQGGSATH
                     THTASATVSGTATANVVANGGTGVAIDTHSHTMTPTIGSSSSLPAYRQLRYIQANSAG
                     DPGTIPAGAILIFDGSLPAGWTRYSALDDRYPRGENTVGTNGGSNTHTHTITGSTDAA
                     SGSTYSRRNGGTQVSAATDNHTHTISSNTASESLEPPYITVVFATSSVATATPQNAIA
                     MWSDTPPAGWLDRSSSADKPFYNRYIKGNATYGTTGGADTHTPADVLGITTSAAVGTI
                     NSRSGGAGAAASHTHNVDVTNFTAAANTPPYTSVIFGKFFGYVPIYDQTAYRWYANQN
                     AQTPTDPWPSGGDDIDESTPIDTTLTPVAVGDVVRLRLQLSVTNSTTTGESFKLQYGT
                     TSADCAAVPTWTDVGQSGSSTAWIGFNNSGVADGGTLSSSTLTNTTALESYEEFNPSV
                     ALPSAVPAGGYGEWDYVLKQNGAEAGTNYCFRMVESDGTELFQYTQYPMLVTNAAPDA
                     PAQSKLFDNEAVASTSPWFEWTGTDAEADDMTYEIQVDDDSAFGSVNIDNDTATNFAQ
                     FENLSTPADKDPYNNADLIRFKPSSSLANGVTYYWRVRAMDPSGSNAWGSWSATRSFT
                     VDTSVTVSTWFQTTQAQFDTDTLTNAEAEVSGDVRLDNGTSTALYTGSAISTPIDFLD
                     GTVGNSWGSLDLTDSAGLQNSISYTVEYLDESTDTWTAIPSSDLPGNPGLSTLNGDFP
                     LSLGGLDTETYRKIRLVADLGQLNGGSTPFLYDWTVTWGYRVETPATTMPFDNEKVGT
                     TTPTFEFTTTDPQSDDLEYQIQWSTSYAFTASTTRSSSVNAGFVDTANGVDTSPFASG
                     DTIQYTIQPSDALTNGTTYWWRVRARDPLGSNEWSFYADPQSLTVDVTTSVSTWYQTT
                     ASQFDTDILSGTVSTAANTATVATTSKETLVAYAEGTQTTPRYRIWDGTTWSTEDSAL
                     DVGAAQTWVVTKAAPTRSEYILATLGTDADVNVQVYEDGAWGDLQEVTTSNANTSMRG
                     FDVAYETQSGDALVVYCDGDADPSYYVWNGTSWTSGGNIGLTSGNTCGWVKLISDPTS
                     DEIIAVTRDLDGTSYEARVWDGSAWGNSATWGSMQAAQTTHEGIAAEYEESGDQAVVA
                     VSNGTNSRFQWRAWNGSAWSATANQTLGDDFESGVIARDVGSDNMAICYMDQDGDLGV
                     VRWTGAAWTGQTELYTGWAQGAQFADRPIDCAYEVGGSRDGDLMVLYSTTAALNFQYF
                     DGVGWSVAAAASSIQDSPRVELRRTGDNLLQGIAYDSTNDRYDYTYWNGSTWSARQAI
                     ETDGAAGGSPFKEPFMIAPKYPSTSGAVIGDPAIDFYAGSGPYWQTFSWNDAQPGGST
                     ANYQIEYFDGTDWQLVPDALLPGNSSGLTSSPVSLAGILPVATYNMLRPVANLTCNGA
                     SCPSLSDWTIEWSAGITISGTAKQFDETTNVTSGSVAVAVNGTLQVGKTGTISGGTWS
                     ISNVNTAPGDIVTVFINGANDANEAVAVARYDGVGDMDGLKLYEQHLTLGSNDATSTP
                     FTNADIGSYDFTQDEDLFFDASGSSLDMCAETGCEADRLFVASTTTYAPGGTTDLGNV
                     TNAGTLSLGGNAIRASRSWLNTGTTSAGTSTVTFTATSSAETIDETGAVAASFYNVSF
                     GSTTAGGTWTLLSPLTASNNLTVSRGTLARDGEAITVGGSLVNGATASWTGIGTTTFN
                     GSGTNTWTDSNPTLQNVGRVVVDGTSKTIQLGSNVKAESMKIGADDILDASAGNRDIT
                     VLGDWVNQNAFVAHQGEVFMSATTTNHIVTAGGDAFYDLTFNGAGGAWSFTEGDLAVN
                     DNFTVSTGTVTMPTGTTTVAGSWNSAGGTFAHNNGTISMTASGAKTVTASGTPFTNNF
                     YTFALNGSGSLTFLDQNATTTNDFRIKQGSVTLPSGTLSVGGDFANTFGSFSHNSGTV
                     KFTSSSAKTIDTNASFANLSFTGSGSWSFTDASVTAVGSLLVENGTLTLPSGTLTLGG
                     SFSNAATVTHNNGTVLFNSADGGETIDLGTSSLYDMTFQSTTGGWTITSSATTTHNTT
                     LTTAGSFTLAAGETLSVGGTFTNAVGGASTTWTGSTLSLQSGSYSINAKTNAGDEYET
                     LRLRPGAHIKMWNSSAASYDIDPIASLYSEDHAGVDGDLYIFGFYANTGDEYWSYATD
                     FDGTALTASTSRQANVKFASGASASLTGGRFEVVGAAGASTTVGNQGSGTYTVAASAG
                     TTTMNYYDVNDLGSTGISLSGTGTVVSLDHGAFTPAFSGGTALTVASSTIGANPGLQI
                     FGVDFSTTTAISASNVTQTDDTPSSYWWFRSSTGNIDGEAFDDDTGDPGSIRWDDSSL
                     VITVSGHVYADDGVTPLGSPTCDDVTANVRIVVSGGASYTGSCSSADGSYSIPGVVVV
                     GDPVLTVYLDTNGGEHGAVVTRTPTTDISDLDIYENRLTTREEDVAPLTILNMAVYDH
                     DDDADLPFVAATGTLTVFADTELHIASSTTFDPAGDITIGANASGTPFDGSLHIDDNA
                     TLLGFATSTITVGGSFTMDQGATFLAASTTVVMNATTTGKTITTPAGQTIGFDTLQFT
                     GVGGGWNINGDISARSGIDVTTGTVSGTGDITVTNGSLSGDGTLSMGSGTTTVETTNT
                     LGGATPWTFANLVLGNGAVTGTTTPGSNATTTVLGKLTIATGHFLDAGGSRWNLAGTG
                     NVFSEQGTFLEGTSTVRYSGTGAANILSTNYYDLEMKAQGGAPTYVATGLGIVVRNDL
                     TVGGATTTKVTFDASDPALDVMGNVVIDSTGTLIGSASAVTTVEGDWDNNGTFTASGG
                     TVTFDGAGASDVFAGGSPFANLTVSAGGSVTIGEPATTTGTFLLSSAGAFSVGSGETL
                     AVGGTFDNRVGGAATTWAGSTLYLYGGGNYTMNASTTADVYGTLRVGAGTEPRMWNSS
                     ASTYDVASTGSLYSEDHANVDGDLYIFGSYVKASGTDHWDYATDFDGTPLGSPRTVNV
                     RIASGGSVLYTGGGLSVVGTTTGTTTIENQGSGTYSLRIAGTASTTWSYYRVRDMDSS
                     GLTFSGTPTVSTLSNGDIAVGVAGGSAMTVGGTVITQNPAMNFTNDRFGTTTGVSPAV
                     NVTATGTTVSSWRFTNHTGEIAGEAFDVDPDGDPGYIVWDDSAALITISGHVYSDEGS
                     TVSAACDGATANIALRVAGLTSYATSCDAGTGAFSISGVAFSPNDSLVVYIDGESEKA
                     AAITADPVSNIADMDLYENRVIVRHENTDPLTIDDMAVWDSSDDADIPFTAVSGSPDT
                     LTLPADTKLIVWTGKTFRPGGNVTVPGGGGGAAYDGTLELFANATFDATGAETHTIGG
                     SLIADNGASLDDETSTFVFTTTGAGRTIDTNEDPLYNMTLNGSGSWTVSNAALEVGND
                     LTVTLGAVTLPAATTTIGGSLLVTGGSFDPNGGTMVFTSSGAESINAATSSFAGLAIL
                     GSGNFTMGGASATATKGVEIDQGTLMSVSGTFSIGESFENVGGSFTHNNGTLRFTSAT
                     GATVTASGSDLGSVTFAGAGAHVFTDANLALRGSLRIEAGSVQLPLGTMSVGGSFVNS
                     GGSFTHATGTILFNSTDAGETVDPGGSPFNALTFASASGGWTILSDATTTGNFALTAA
                     SSFTLASSTRLSVGGVFTNLVGGAATTWTGSTVTVNSGTGYTINSKTQGGDVYNKLEI
                     GSSTALRMWDSTGAVTMLDSASSLYSEDHAGVSGSLYIFGNYVRSTGADYWSYATDFD
                     GTALGGSSRDAAVYFAEGASTTITGGTLNIVGASGHKTAISNQGSGTYGLSVMGGTLN
                     ASNFSLANMGAAGLSLSGNTTVTSLSQGDFTLAVSGGSLITLSSTTLNFNAGLVSTGD
                     TFATTTAITGYNVNLIGTTPTAWTFTGTSGNFDGEAFDNDGVDDCGSIRWDDSTCLVT
                     SQSAYRWRNDDGGEGVPDSEWYDVNWTKRKRVTVHNADPVSYADATVKVDVPYDADMQ
                     ADFDDLRFTASDGVTELDHYVESYTASTDAVVWVNVPTLATSTDTTVYMYYGNSGATS
                     GNATTTFPYLDDFEDGDIAEYSGDTTLFTVDGTFAYGGVNGLDATGHETEKATDGVYR
                     TDVTVSQGEAIRFMQYIDTAAGSGDETCTLFGVQSPGSSNQNYAVCLELFGTDRISIS
                     KDVSNNDTSGTVLATSTVTYETGWYEVETAWEASGTIAVTLSKDGSVVATTSANDSTY
                     SSGGMGFTFWFQNGGWDDYSARTLLTTEPSVTLGSEQVPGGASWLAALNAPATGVNTG
                     ENARVRFLIENSGLPITNQDFELDYAPKGASPSCEAVSYGDYVPVPPSASCGTNDLCM
                     QTSANLANHEATTDLLGGDGTFTPGEAIEDPSNTTNSLDIAQDSYTELEYVVTPTVNV
                     TDSNYCLRVTNAGSDLDQYTRMAELQLVFAPNITSLSLNGGMDIALTGGSTTTVYATG
                     TVSDLNGYTDLEFATTTIFRSGVGESCTPDNNNCYVASGPQCTFTNCSGTSCDVECSA
                     DIYFHADPTDVGTFAGETWRAMLAIEDGSGLVATATAPSVDLLTLRAISVDDSIDYGV
                     LAVNSDTGSYNASTTVENIGNDAIDVDVEGTDLTDGGSSAIPVYEQKFATSTFTYSSC
                     VFCSQLSTTTSHVELNLSKPTSTAPAITDHVFWGIAIPFGVAGTPHSGVNTFYAVGD"
     gene            18797..20272
                     /locus_tag="VFS75_03640"
     CDS             18797..20272
                     /locus_tag="VFS75_03640"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU4677780.1"
                     /translation="MMRSFSSALLATMAVLWCANLFPIAEAADITPPAISNVEVANVS
                     NDTVTITWKTDENADATVNYGLQEDYGIVRVAGATTTTHSVTLSKLEVGRTYYFRVVS
                     SDEAGNQGISANYKIVTEGTPQSGDGSQSSTETQSKTTQEIVQEIQQVQSPQQLQEII
                     NQTTEALQGITKDLTIVGPPTVIAETTSATIKWTTDRESSSVVLFSPSDSYVADQYQY
                     SQASTGADTTDHEVRVIGLEPYTEYHFKVQSKDAYAITGESRDYTFRTKASMPDIKNL
                     HIVKVEENSATLAWDTTVPAKATIEYQDMTTGAQNSVGRPTLATTNQMRISDLTLGTR
                     YVAFVIAENAGGDRVKSQPITFVTVKDTAAPIISNVTNESTLFPGGESRIQTIIEWLT
                     DEPAHCSLTYQEGIAGGIEPTTIDSKDSGYSAKHTEVIVDFAPATVYQFWLTCADEAG
                     NSVKSENYVLFTPIQEKSIIDIIIENFSSTFGWVKNIGGGG"
     gene            20288..>21562
                     /locus_tag="VFS75_03645"
     CDS             20288..>21562
                     /locus_tag="VFS75_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4677781.1"
                     /translation="MAKPLIVCRNLDITYNKGKSNEYKALQNVNTDIYEGEYIILFGA
                     SGSGKSTLMYAIQGSLPPGGGTLLIRGDDIYSYPPQERVYFQRYIMGIIFQSFNLIGS
                     LTVLDNVALPMIFCDANKASRERRAQMLLDRFGVGHVSHKVPSMLSGGQQQRVSVARS
                     MVNDPQILLADEPTGNLDSISTQQVMDKIDEINTFDQRTIIMVSHNAAHLSYAHRVYY
                     LKDGIVVREVVNPQRKQIKPVKEGETIVTELEQLARLFPYDSVDTLRVKSLTNYLTQQ
                     YTFDQLIRLEKAVNLLIKGKIRKRQFIATLIKKYEDGGVGIDRKTADHMAEVSVALVT
                     QADDVRRFRSDKQQQSEAAFFAQHKFAERVREYLLMHHRVPLSRAMEANLTTMIADRL
                     SGVSGEEEFYDALGAPVKEGGLGLERKVVSDIS"
BASE COUNT         4289 a         7059 c         6696 g         3518 t
ORIGIN      
        1 tacgcccgga ggagaaggag aaagtgaagc tcaagatcaa caaggtacgc ggtaagacgc
       61 gttaagacgg aggggaagga ccaccatgca agttcttaga gttcattcga tacggagagc
      121 gttcacctac accctcgcgg cactggtttt tttcatactg cccggcgtca tccatgcggc
      181 gaccgtcaac atatcaccgt cgaccggttc ctacggcgtc ggccaatcat tcacggtgaa
      241 cgtccaagtc gacccagaag ggaaaagtgc caatgcggct caggcacagt tgacgttcga
      301 taaaacgaag ctctctgtcg tcaacatcac gaagacgcag acattcccat tttggacgac
      361 cgaacctaaa ttctcgaata ccgacggtac catcgacttc ggcggcggag ggccgacccc
      421 gttcagctcg aaactcaccg tcgtgaccat aaatttcaag gcgatcgcgg aaggctcggc
      481 acaagtgtcc gtaagttcgg cggaggtcac cgcggcggac ggtttgggca ccgacctcta
      541 ttcgggtccg gggaacggca ccttcaccat cactgcggca tcgacgaaac ccgcggagaa
      601 ccctacaccg acgacgtcag agacgccgac tgacggcggc tccgatagca ccgacggccc
      661 gatcaccttc ggcgacccac cgcgcgcacc agaggtcggc tcgaccatct tcatggaccc
      721 ggagacgtgg tacgcgacga aggtcggtac gttcacgtgg agcgttccct tcgacgtcga
      781 cagcatggcg ctcgacatat cgacctcttc ggaagccgag gcggtgacgg tcatggaccc
      841 gcccatcgat tcgatcgcgc tcactgagga aaacctcatc gacggtgttc aatacctcac
      901 gatgaagtac aagaaccagg tcgactgggg agcgacgctc caccggaaga tcatgatcga
      961 caccattccg cccgaaccgt tcaaggtgaa cgtgaacgcg gggaactcac cgtcgaagtt
     1021 cccgctcctc accttcgagg cgcacgacga accgtccggt atccagaagt atcaaatggc
     1081 gatcgcgggg ggcgagccgt tcgacatcac gccggatgag gcgaagctcg ggtacctcct
     1141 caaggatctc gaggacggca cgtacacggt gaaggtcgtc gcgttcgact acgcggggaa
     1201 caagacggag gcgaccgcgg cgattctcat caccgcgggc tggcacaagc cggtcgagac
     1261 ggtcattacg acgtcgatct gggacttctt caaggggaag aacctcgtca tcatcctcct
     1321 ccttctcgcg ctcatcgcgc agttcgtcct cgcgcactat gcgcgtaagc aacacgaact
     1381 tcgcgagcag aagctccgaa aggagacgaa ggagatacag gaccagatgg agaagatctt
     1441 ctccgcgctc cgcgacgaga tctacgatca gatcaacgcg atcacgaaga acgaacggct
     1501 ctcgaagaag gagaaggaag ccgtcgaagg actcaaccag gcgctcgaag tctccgaaac
     1561 cttgatcgaa aaggagatca ccgacgtgag caagatactc aagtaggaaa gcgcactcga
     1621 atccacacct atagcgcgtc aaaccacggt ttggcgcgct ataattatac ttaacccgta
     1681 ctctaccttg gtactgggtt gcttcctcgt gaaggtgata aaatcctcac ccgaaaagag
     1741 aagagagaga aataaaacgt tatttagagc gatagaatgg agaacgcgcg cttttcgtag
     1801 tgccatagcg tatttcatac cgaaccccat tccgcttcgc ggccacgtca gcgtccgtag
     1861 gatcgcgcgc ggacgcgcgc ttcgcggtgc actcgcaagc atggcgctcg ccgttttcgc
     1921 attcaccatc acttccgtcg cggagtcaaa cttgagtccg gtgcgcaaac tcgacacgct
     1981 gaacgtcctt ccgacgagcg tcgagagcac ctcgtggcat aggccggagg aggcgctcgt
     2041 ccaagacgtc gacgagagcg gcgtgtatca aagcttcacg aagacgaact cgtcgtacta
     2101 cgacggaaag gatccgaagg acgacgtcct gccgcaaccg acgatcggca cgccaagcac
     2161 ggatgctcac accgacgcat cgtcgacctc ggccgaatcg gacgtcgata cgtcggacga
     2221 tgcacccgcc ggcgattcga ccgagacgtc gacgccggtc tccgaggatg acgcggcgat
     2281 ggacgcttcg actgggacag aggagcatca agcgagcgaa ccgtcgacgg atgcaagcgc
     2341 accgtctgac tcaccgagcc aaagcgaagc gcctgaaccc gccccgtcag aaccggttac
     2401 ggtgagcgcg ccggcggcga agagcgactc gggcgcgtcg agcgcggagt cgccgcttca
     2461 ggccctcagc cgaagtttct tccgcatcac cgatgtcgcg ctctcgcttc tcccgttcgc
     2521 ggacgtttcg acgagcgccc ccgtgaccac ggtcgacggg tcatccgtac cggagagcgc
     2581 cggctcgccc acagccgaga cctccacacc agagagcgtc gacgcgcccg atatggacga
     2641 gactcccgtg agcgaggagc cggcgaacgc ggacgcggga gcgccagagg gaactcccat
     2701 ttcggagcac gcatcatcgg acggggcgaa cgagaccgcg acctcgagcg aggagaccgc
     2761 gggcgagcct gcggtgacga cgccgagcgc tttacgggcg acgtcgaccg atacgggcat
     2821 cacgttcgag catttcgaac taccgccgct tgaacctggc cagctcattc gaaacgtcca
     2881 gctccgcatg tcgctcgccg gacaggtcga tcttcccgaa ggcgtcgccg ctccctctct
     2941 cgaggtcgag tacgacaatg gctccggctg gacgagcgcg ggcgcggtgc ttctcgacgg
     3001 cgaggtgtcg aacgcgctca atgacgggta cttcctcttc gcgctcccgc gcttcctctc
     3061 ggtcgattcg ctcgaagatt tcaaggtgcg cgtcacctac cgcggcgacc aggaagccct
     3121 caagcgcgta ttcgtcgatt ccgtgtggct ccaggtcgac acggagacgt tcaatcgcga
     3181 agtcctcaag gagcgtctcc ttccgtccga gctcgcgcac cttacgcacc catcgatgta
     3241 cgagttcctc ggcaccgacg tcgacttcgc gcgcggagac gtgccgcgct tctcgctcaa
     3301 gtacgagtcc cagcgaaacg ccgcgatacg gttcatccgt aacctcttcg gacgcgacct
     3361 cgcctcgctc caatcggtcg tcgtccgcaa gcacagcggc gagccgatcg acgtccaccc
     3421 cgacgtcaac gtgaccgccg acggcctcat ttccctacaa ctctcgcccg aggacacggc
     3481 gtccctcgag ccgggcgcgt actcgatcga gctttccgta gacgagggcg gcaaggtgtt
     3541 caccgactcg ttcgagttcc agtacggtct cgtcgcgatc aaccccgacc agaccgaata
     3601 cgcgctcggc gcgacgacga ccatcatgat gggcgccgtt tccgagggcg gtaacacgct
     3661 ctgcggcgcg gacctccacc tctacgtcat cgaccctgcg ggcgccgtcg tcgaggttcc
     3721 cgtcacggca tcggccgcgt gcgacggcaa taacctcgtc gacgcgcccg actacgaggc
     3781 gacgtacgtc gcgagcgcgc ccggcacgta cgagatgtac gccgagcgga tcggcgcgga
     3841 cggcgaggtc atggcacacg cggacgacac cttcaaggtc gtcgcgaagc agggcttcac
     3901 gctccgacgc gacgggccga cgcgcatcaa cccgaagtac gactacccga tgacgttcac
     3961 gctcacgacg gcgaaggcct tcacgggtga gctcgtcgag cgcgtgccga aggacttcgc
     4021 gatcgtctcg accgacgcct cggtccgtga cgcgggtgag taccaagagc ttcgcttcgc
     4081 ggtctcccag aaggcggggg agacgatgac ctacgcgtac cgattcgatg cgccggacat
     4141 ctcgccgtac ctctacaacc tcggtccggc gacgctcgtc gacgatgcga acatcgtcgt
     4201 cgtccgagag acgctcgatg gcgaaagcgc gttcgcggag gaatccgtga cgaccggcac
     4261 gtcgacggtc gatgaggcat cgaccgcgac cacggacacg actggggtga tggaggtgtc
     4321 accgacggag ccagagagcg acgcgtctac ggcggatacg tctccggaac ctgactccgc
     4381 ggcgcccgat acgacgagcg agacgcctac gcccgccgtc gacgccgcga gcgagccgac
     4441 cacgccggcg acatcgacac cgaccgacgc acccgcgagc gacgcggtcg ctcccgcctc
     4501 acccgccgcg atacccttgc cgagcgcgcc ggtcgcgacc gataccgcga cgagcaccga
     4561 tatcgtcacg agtccgacga acgccacatc gaccgtgagc accgcgacgt cgaccggcga
     4621 gaaggcgagc ttcgccgagc accgcgcgtg gcagatagcg tccgacgcga cgggaagcat
     4681 gatcgtcctc tggacgagcg gcgcctcgat accggccgga tggacgtgta tctcgtgcgc
     4741 ggcggggaac acgttcttcc agcgcttccc ggtcggcagt tcgacgtacg gcaatcaggg
     4801 cggttcggcg acccacacgc acaccgcgtc ggcgacggtg agcggaacgg cgaccgcgaa
     4861 cgtcgtcgcg aacggcggta ccggtgtcgc gatcgacacg cactcacaca cgatgacgcc
     4921 gaccattgga agttcctcat cgctccccgc gtaccgccag ctccgctaca tccaagcgaa
     4981 cagcgcaggc gacccgggaa cgattcccgc gggtgcgatc ctcatcttcg atgggtcgct
     5041 ccccgcgggc tggactcgct acagcgcgct cgacgatcga tacccgcgcg gcgagaacac
     5101 cgtggggacg aacggcggct cgaacacgca cacgcacacc atcaccggtt cgaccgacgc
     5161 ggcgtccggt tcgacgtact cgaggcgaaa cggcggtacg caggtgtcgg ccgcgacgga
     5221 caaccacacg catacgatct cgtcgaacac ggcatccgag agcctcgaac cgccgtacat
     5281 aaccgtcgtc ttcgcgacgt cctcggtcgc gaccgcgacg ccgcaaaacg cgatcgcgat
     5341 gtggtcggat acgccgcccg caggctggct cgaccgctcc tcgagcgccg acaagccgtt
     5401 ctataaccgc tacatcaagg gtaacgcgac ctacgggacg acggggggcg ccgacacgca
     5461 cacccccgcg gacgtcctcg gcatcacgac gagcgcggcg gtcgggacga taaacagccg
     5521 ctcgggtggc gcgggcgcgg ccgcgtcgca cacgcacaac gtcgacgtga cgaacttcac
     5581 cgccgctgcg aacacgccgc cctatacgtc ggtcatcttc ggtaaattct tcggatacgt
     5641 ccccatctat gatcagacgg cgtatcgctg gtacgcgaac cagaacgcac agacgccgac
     5701 cgacccctgg ccatcgggcg gagatgatat cgacgagagc acgccgatcg acacgacgct
     5761 cacgccggtc gcggtcggcg acgtcgtccg tctccgtctc cagctctccg tcacgaactc
     5821 gacgacgacg ggcgagagct tcaagctcca gtacgggaca acgtccgccg actgcgccgc
     5881 cgtgcccaca tggaccgatg tcggccagag cggttcgtcg accgcatgga tcggtttcaa
     5941 taactcgggc gtcgccgatg ggggtacgct ctcgtcttcg acgctcacca atacgaccgc
     6001 tcttgaatcg tacgaggagt tcaatccgtc ggtcgcgctc ccgagtgccg tgcccgcagg
     6061 cgggtatggc gagtgggact acgtcttgaa gcaaaacggt gccgaggcgg ggacgaacta
     6121 ctgtttccgt atggtggagt ccgacggtac cgaattgttc caatataccc agtatccgat
     6181 gctcgtcacg aacgccgcac cggacgcgcc cgcgcagtcg aagctcttcg ataacgaagc
     6241 ggtcgcctcg acgtcgccat ggttcgagtg gacgggtacc gacgcggaag cagacgacat
     6301 gacctatgag atacaggtcg acgacgactc ggccttcggg tcggtcaaca tagacaacga
     6361 caccgcgacg aacttcgcgc agttcgagaa cctctcgacg ccggccgaca aggacccgta
     6421 caacaacgcg gacctcatcc gcttcaaacc gagctcgtcg ctcgcgaacg gtgtcaccta
     6481 ctactggcgc gttcgcgcga tggacccgtc gggttcgaac gcgtggggaa gttggtccgc
     6541 gacgcggagt ttcaccgtcg atacgtccgt caccgtctcg acgtggttcc agacgacaca
     6601 ggcgcagttc gacaccgaca cgctcacgaa cgcggaggcc gaggtgtcgg gcgacgttcg
     6661 actcgacaac ggcacgtcga ccgcgctcta tacgggtagt gccatatcga caccgatcga
     6721 cttcctcgac gggacggtcg gtaactcctg gggttccctc gacctcaccg attcggcggg
     6781 actgcaaaat agcattagct atacggtcga gtacctcgat gaatcgaccg acacgtggac
     6841 cgccattccg agtagcgacc ttcccgggaa ccccggcctc tcaaccctaa acggcgactt
     6901 cccgttgagc ctcggtggac tcgataccga aacgtaccga aagattcgac tcgttgccga
     6961 tctgggtcag ttgaacggcg ggtcgacgcc gttcctctac gactggacgg tcacgtgggg
     7021 gtaccgcgtc gagacgccgg cgaccacgat gcccttcgac aacgagaagg tcgggacgac
     7081 gacgccgacc ttcgagttca cgacgaccga cccgcagtcg gacgatctcg agtatcagat
     7141 acagtggtcg acgagctacg cgttcaccgc gtcgacgacg cgaagctcga gcgtgaacgc
     7201 cgggttcgtc gatacggcga acggagtcga tacgagcccc ttcgcatcgg gcgatacgat
     7261 ccagtacact atccagcctt cggacgcgct cacgaacggc acgacgtact ggtggcgcgt
     7321 ccgcgcgcgc gaccccttgg ggtcgaacga gtggtcgttc tacgcggacc cacagagcct
     7381 caccgtcgac gtcaccacct cggtctcgac gtggtaccag acgaccgcat cgcagttcga
     7441 tacggacatc ctctccggca ccgtctcgac ggcggccaac acggcgaccg tcgcgacgac
     7501 ctcgaaggag actctggtcg cgtacgcgga aggcacgcag acgacgccgc ggtaccgcat
     7561 ctgggacggg acgacgtgga gcaccgagga tagcgcgctc gacgtcgggg ccgcgcagac
     7621 gtgggtcgtc acgaaggcgg ccccgacgcg aagcgaatac atcctcgcga ccctcggtac
     7681 ggacgcggac gtgaacgtcc aggtgtacga ggacggcgcg tggggcgatc tccaagaggt
     7741 gacgacgtcg aacgcgaaca cctcgatgcg cggcttcgac gtcgcgtacg agacgcaatc
     7801 gggcgacgcg ctcgtcgtct actgcgacgg cgacgccgac ccctcgtact acgtatggaa
     7861 cggtacgtcg tggacctcgg gcggtaacat cgggcttacc tccggaaaca cgtgcggctg
     7921 ggtaaagctc atctccgacc cgacctccga cgagatcatc gcggtgaccc gcgacctcga
     7981 cggtacgtca tatgaggcgc gcgtctggga cggttccgcg tggggaaatt ccgcgacgtg
     8041 ggggagcatg caggccgcgc agacgaccca tgaaggtatc gccgcggagt acgaggagag
     8101 cggcgaccag gcggtcgtcg cggtctcgaa cggcacgaac agccgcttcc agtggcgcgc
     8161 ctggaacggc tccgcatggt cggcgaccgc gaaccaaacg ctcggcgacg acttcgagtc
     8221 cggcgtcatc gcccgcgacg tcggctccga caatatggcc atttgctata tggaccagga
     8281 cggcgacctc ggcgtcgttc gttggaccgg tgccgcgtgg accggtcaga cggagctcta
     8341 cacggggtgg gcacagggtg cgcagttcgc ggaccgaccc atcgattgtg cgtacgaggt
     8401 gggcgggtcg cgggacggtg atctcatggt tctctacagc acgaccgcgg cgctcaactt
     8461 ccagtacttc gatggcgtcg ggtggtcggt cgcggcggcc gcctcgtcca ttcaggattc
     8521 gccgcgcgtc gagctccgcc gcacggggga caatctcctc cagggtatcg cgtacgacag
     8581 cacgaacgac cgctacgact acacgtattg gaacggttca acgtggagcg cgcggcaggc
     8641 gatcgagaca gatggcgcgg cgggcggttc gcccttcaaa gagccgttca tgatcgcgcc
     8701 gaagtacccg tcgacgtcgg gtgcggtcat cggcgacccc gccatcgatt tctacgcggg
     8761 tagtgggccg tactggcaga ccttctcgtg gaacgacgcg caaccgggcg gctcgaccgc
     8821 gaactaccag atcgagtatt tcgacggcac ggattggcag ctcgtgcccg atgcgcttct
     8881 ccccggaaac tcctcgggtc tcacctcgag cccggtcagt ctcgccggca tcctcccggt
     8941 cgcgacgtac aacatgcttc gcccggtcgc gaatctcacc tgtaacggag catcgtgtcc
     9001 ctcgctctcg gattggacca tcgaatggtc ggcgggtatc acgatatcgg gtaccgcgaa
     9061 gcagttcgac gagacgacga acgtcacgtc cgggtccgtg gcggttgccg tgaacggcac
     9121 gctccaggta gggaagaccg gtaccatctc gggcggcacg tggtccatct cgaacgtgaa
     9181 cacggcgccg ggcgatatcg tcaccgtctt cataaacggc gcgaacgacg cgaacgaggc
     9241 ggtcgcggtc gcacggtatg acggcgtcgg cgacatggac ggcctcaagc tctatgaaca
     9301 gcacctcacg ctcggctcga acgatgcgac gtcgacaccc ttcacgaacg cggacatcgg
     9361 ttcgtacgac tttacacagg acgaagacct cttcttcgac gcgagcggct cttcgctcga
     9421 tatgtgcgcg gagaccggct gtgaggcaga tcgcctcttc gtcgcctcga cgacgacgta
     9481 cgcgccgggc ggcacgacgg acctcggaaa cgtcacgaac gcgggtacgc tctcgctcgg
     9541 cggaaacgcc atccgcgcct cgcgctcgtg gctgaacacg gggacgacga gcgcggggac
     9601 ctcgaccgtt acgttcaccg cgacgtcgag cgcggagacc atcgacgaga ccggcgccgt
     9661 cgccgcgtcc ttctacaacg tctccttcgg gtcgacgacc gcgggtggaa catggacctt
     9721 gctctcgcct ctcaccgcct cgaataacct caccgtctct cgcggtactc tcgcacgcga
     9781 cggcgaggcg atcacggttg gtggctccct cgtaaatggc gcgaccgcaa gttggaccgg
     9841 cataggaacg acgacgttca atggttcggg aacgaacaca tggaccgatt cgaatccaac
     9901 cctccagaac gtcggtcgcg tcgtcgtcga cggtacctcg aagaccatcc agctcggctc
     9961 gaacgtgaag gcagagagta tgaagatcgg cgcggacgac attctcgacg cgtcggcggg
    10021 gaaccgtgat atcaccgtcc tcggtgactg ggtgaaccag aacgccttcg tggcgcacca
    10081 gggcgaggtc ttcatgagcg cgacgacgac gaatcatatc gtgaccgcgg gaggcgacgc
    10141 gttctacgac ctcacgttca acggcgcggg cggcgcatgg tccttcacgg aaggcgacct
    10201 cgcggtgaac gataacttca cggtctcgac gggtacggta acgatgccga cgggcacgac
    10261 gaccgtcgcg gggtcctgga acagcgcggg cggcacgttc gcgcacaaca acggcacgat
    10321 cagcatgacc gcgagcggcg cgaagacggt gaccgcgagc gggacgccgt tcacgaacaa
    10381 tttctatacg ttcgccttga acggttcggg aagccttacc ttcctcgacc agaacgcgac
    10441 gacgacgaac gatttccgca tcaagcaggg gagcgtcacg ctcccgtcgg ggacgctctc
    10501 ggtcggaggc gacttcgcga acacgttcgg ctcgttcagc cataacagcg ggacggtcaa
    10561 gttcacgagc tcatcggcga agaccatcga tacgaacgcg tcgttcgcga acctctcgtt
    10621 cacggggtca ggctcctggt cgttcaccga cgcgtcggtc acggcggtcg gttccctctt
    10681 ggtcgaaaac ggaacgctca cgctaccctc ggggacgctc acgctcggcg ggtccttctc
    10741 gaacgccgcg acggtgaccc ataataacgg gacggtcctc ttcaattcgg cggacggtgg
    10801 cgagaccatc gatctcggga cgtcgtccct ctacgacatg acgttccaga gcacgacggg
    10861 aggctggacc atcacgtcct cggcgacgac gacgcataac acgacgctca cgaccgccgg
    10921 ctccttcacg ctcgcggcag gagagacgct ctcggtcggc ggcacgttca cgaacgcggt
    10981 gggcggcgcg tcgacgacgt ggaccggctc gaccctctcc ctccagtcgg gaagttacag
    11041 cataaacgcg aagacgaacg caggcgacga gtacgaaacc ttgcgattgc ggcccggcgc
    11101 gcacatcaag atgtggaact cgagcgccgc ctcgtacgac atcgacccga tcgcgtccct
    11161 ctactccgag gaccatgcgg gcgtcgacgg cgacctctac atattcgggt tctacgcgaa
    11221 cacgggcgac gagtactgga gctacgcgac ggacttcgac ggaacggcgc tcaccgcatc
    11281 gacgtcgcgc caggcgaacg tgaagttcgc gagcggcgcg agcgcgtccc tcacgggtgg
    11341 acgcttcgag gtcgtgggcg ccgcaggcgc gtcgacgaca gtcggaaatc aggggagcgg
    11401 cacgtacacg gtcgccgctt ccgcggggac gacgacgatg aactactacg acgtgaacga
    11461 tctcggctcg acgggcatct cgctctcggg gaccggtacg gtcgtttcgc tcgaccacgg
    11521 cgcgttcacg cccgcgttct ccggcggcac ggcgctcacg gtcgcgtcgt cgaccatcgg
    11581 ggcgaaccct ggactccaga tattcggcgt cgacttctcg acgacgacgg cgatcagcgc
    11641 atcgaacgtg acgcagaccg acgacacgcc gtcgtcgtac tggtggttta ggagcagtac
    11701 gggaaatatc gacggtgagg cgttcgacga cgataccggc gacccgggtt cgatacgttg
    11761 ggacgatagc tccctcgtca tcacggtctc gggacacgtc tacgcggatg acggcgtaac
    11821 gccgcttggc tcgcctacct gcgatgatgt gacggcgaac gttcgcatcg tcgtctcggg
    11881 cggcgcttcc tacacgggga gctgttcgtc cgcggacggg agctacagta ttccgggcgt
    11941 cgtcgtcgtc ggcgacccgg tccttacggt gtacctcgat acgaacggcg gtgagcacgg
    12001 agcggtcgtt acgcggacgc cgaccaccga catcagcgac ctcgatatct atgagaatcg
    12061 gctcacgacg cgcgaggagg acgttgctcc gcttaccatc ctcaacatgg cggtatacga
    12121 ccacgacgac gacgccgacc tacccttcgt cgccgcgacc ggcacgctca ccgtgttcgc
    12181 cgacactgag ctccacatcg cgtcgtcgac gacgttcgac cccgctggcg acatcacgat
    12241 aggcgcgaac gcgtcgggaa cgccgttcga tggctccctc cacatcgacg acaatgcgac
    12301 gcttctcggc ttcgcgacat cgacgatcac ggtcggcggt agtttcacca tggaccaggg
    12361 tgcgaccttc ttggccgcct cgacgacggt cgtcatgaac gcgaccacga cggggaagac
    12421 cattacgacc cccgccggac agacgatcgg tttcgatacg ctccaattca ccggtgtggg
    12481 cggcggctgg aacattaatg gcgatatcag tgcgagatcc ggcatcgacg tcacgacggg
    12541 aacggtctcc ggtaccggcg acatcaccgt gacgaacggt tcgctctccg gagacggtac
    12601 gctgtcgatg ggctccggca cgacgaccgt cgagacgacg aatacgctcg gcggcgcgac
    12661 gccgtggacc ttcgcgaacc tcgtcctcgg gaacggcgcg gtgaccggta cgacgacgcc
    12721 gggttcgaac gcgacgacga ccgtccttgg gaagctcacc atcgcgacgg gacacttcct
    12781 cgacgcgggt ggttcgcgtt ggaacctcgc cggcacgggt aacgtcttta gcgagcaagg
    12841 tacgttcctc gagggtacgt cgacggtccg ctatagcggt accggcgcgg cgaacatcct
    12901 ctcgacgaac tattacgacc tcgagatgaa ggcgcaaggt ggcgcaccga cgtacgtggc
    12961 gaccggactc ggcatcgtcg tgaggaacga cctcaccgtg ggaggcgcga cgacgacgaa
    13021 ggtgacgttc gacgcgagcg acccggctct cgacgtcatg gggaacgtcg tgatcgactc
    13081 gaccggcacg ctcataggaa gtgcttcggc ggtcacgacc gtagagggcg actgggacaa
    13141 caacggtacg tttaccgcgt ccggcggtac ggtcacgttc gacggtgcgg gtgcgtcgga
    13201 cgtcttcgcc ggcggttcgc ccttcgcgaa cctcaccgtc tccgcgggcg gctcggtcac
    13261 gatcggagag ccggcgacga cgacgggaac gttcctcctc tcgtcggcgg gcgcgttttc
    13321 ggtcggaagc ggcgaaacgc tcgcggtcgg tggtacgttc gacaatcgag tcggcggtgc
    13381 cgcgacgacg tgggccggtt cgaccctcta cctctatggc ggtggaaact acacgatgaa
    13441 cgcctcgacg accgcggatg tctacgggac gctccgcgtc ggtgcgggga cagagccgcg
    13501 catgtggaac tcgagcgcga gcacgtacga cgtcgcgtcg accggctccc tctattccga
    13561 ggaccacgcg aacgtggacg gtgacctcta tatattcggt tcgtacgtga aggcgagcgg
    13621 taccgatcat tgggactatg cgaccgactt cgatggcacg ccgctcggga gtcctcgcac
    13681 cgtgaacgta cgcatcgcaa gcggcggaag cgtcctctat acgggtggcg gactctcggt
    13741 cgtcggtacg acgacgggga cgacgaccat cgagaatcag gggagcggca cgtacagctt
    13801 gagaatcgcg ggaaccgcgt cgacgacgtg gtcgtactac cgcgtgcgcg acatggattc
    13861 ctccggtctc acgttctccg gcacgccgac ggtatcgact ctctcgaacg gcgacatcgc
    13921 ggtcggtgtc gcgggcggga gcgcgatgac ggtgggtggc acggtgatca cacaaaaccc
    13981 ggcgatgaac ttcacgaacg accgcttcgg cacgacgacg ggcgtctctc cggcggtcaa
    14041 cgtcacggcg acgggcacga cggtgagctc gtggcgcttc acgaatcaca cgggcgagat
    14101 cgccggcgag gcgttcgacg tggaccccga tggcgacccc ggctacatcg tctgggacga
    14161 ctcggcggct ctcatcacca tttcgggtca cgtctacagc gacgaaggct cgaccgtctc
    14221 ggcggcctgc gacggcgcaa cggcgaacat cgcgcttcgc gtcgccggcc tcacgagcta
    14281 tgcgacctcg tgcgacgcgg gcacgggcgc gttctccatc tcgggcgtcg cgttcagccc
    14341 gaacgattcg ctcgtcgtct acatcgacgg cgagagcgag aaggcggcgg cgatcaccgc
    14401 ggatccggtc tcgaacatcg ccgatatgga tctctatgag aaccgcgtca tcgttcgcca
    14461 cgagaacacc gaccccctga cgatcgacga catggcggtg tgggactctt ccgatgacgc
    14521 cgatatcccc ttcactgcgg taagcggctc gccggatacg ctcacgctcc ccgcagacac
    14581 gaagctcatc gtgtggacgg ggaagacgtt ccgtccgggc ggcaacgtga cggtcccggg
    14641 aggcggaggc ggcgccgcgt acgacggtac gctcgagctc ttcgcgaacg ccacgttcga
    14701 cgcgaccggt gcggaaacgc atacgatcgg ggggagcctc atcgcggata acggcgcttc
    14761 gctcgatgac gagacctcga ccttcgtttt cacgacgacc ggcgccggtc gcacgatcga
    14821 cacgaacgag gacccgctct acaacatgac cctcaatggc tcgggaagct ggacggtctc
    14881 gaacgcggcg ctcgaggtcg gaaacgacct caccgtcacg ctgggcgccg tgaccctccc
    14941 cgccgcgacg acgacgatcg gcggctcgct cctcgtgacc ggcggctcgt tcgacccgaa
    15001 cggcgggacg atggtattca cctcgtccgg cgccgaatcc atcaatgcgg ctacctcgag
    15061 cttcgcaggt ctcgccatcc tcggttcggg taatttcacg atgggcggcg cgagcgcgac
    15121 cgcgacgaag ggcgtcgaga tcgaccaggg tacgctcatg agcgtttccg gcacgttctc
    15181 gatcggcgaa tcgttcgaga acgtcggtgg ttcgttcacg cacaacaacg gcacgctccg
    15241 cttcacctct gcgaccggcg cgacggtgac ggcatcgggt tcggacctcg ggagtgtcac
    15301 cttcgcgggc gccggcgcgc acgtcttcac ggacgcgaac ctcgcgctcc gcggttcgct
    15361 ccgcatcgag gccggctccg tacagcttcc cctcggcacg atgtccgtcg gcggttcgtt
    15421 cgtgaactcg ggcggctcct tcacgcacgc gacgggtacc atcctcttca actcgacgga
    15481 cgcgggggag acggtagacc cgggcggctc gccgttcaat gcgctcacgt tcgcgagcgc
    15541 gtcgggaggc tggacgatcc tctccgacgc gacgacgacg gggaacttcg cgctcaccgc
    15601 ggcgtcgagc ttcacgctcg cctcctcgac gcgtctctcg gtcggcggcg tcttcacgaa
    15661 tctcgtcggc ggagccgcga cgacgtggac cggctccacc gtgaccgtca actccggcac
    15721 cggctacacg ataaactcga agacgcaggg tggcgacgtt tacaataagc tcgagatcgg
    15781 ttcgtcgacc gcgctccgca tgtgggactc gaccggtgcg gtcacgatgc tcgatagcgc
    15841 gtcgtccctc tactcggagg accacgcggg cgtctcgggg tcgctctaca tattcggcaa
    15901 ctacgttcgt tcgaccggcg cagactactg gagctatgcg acggacttcg atggtacggc
    15961 gctcggcggc tcctcgcgcg acgccgcggt ctacttcgcg gagggcgctt cgacgaccat
    16021 aacgggcggc acgctcaaca tcgtcggtgc gagcggccac aagaccgcga tatcgaacca
    16081 gggaagcggc acgtacgggc tctcggtcat gggaggcacg ctcaatgcct cgaacttcag
    16141 tctcgcgaac atgggcgccg ccggactttc gctctctggg aacacgaccg tcacgtcgct
    16201 ctcgcagggt gacttcacgc tcgcggtctc gggcggttcc ctcatcacgc tctcgtcgac
    16261 gacccttaat ttcaacgcag ggctcgtctc gacgggtgat acgttcgcga cgacgaccgc
    16321 gatcaccggg tacaacgtga acctcatagg cacgacgccg accgcgtgga ccttcacggg
    16381 gacgagcggt aacttcgacg gtgaggcgtt cgacaacgat ggggtcgacg actgcggttc
    16441 catccgctgg gacgacagca cgtgtctcgt cacctcacag tcggcgtacc gctggcgaaa
    16501 cgatgacggc ggggaaggcg taccggactc ggagtggtac gacgtgaact ggacgaagcg
    16561 aaagcgcgtc acggtccaca acgccgaccc cgtctcgtac gcggacgcga cggtgaaggt
    16621 cgacgtgccg tacgacgccg acatgcaggc cgacttcgat gaccttcgct tcactgcgtc
    16681 ggacggcgtc accgagctcg accactacgt cgagtcctac accgcgagca ccgacgcggt
    16741 cgtctgggtg aacgtgccga cgctcgcgac gtcgaccgat acgaccgtct acatgtacta
    16801 cggcaacagc ggcgcgacga gcggcaacgc gacgacgacc ttcccgtacc tcgatgactt
    16861 cgaggacggc gacatcgccg agtactcggg tgacacgacg ctcttcacgg tcgacggcac
    16921 cttcgcgtac ggcggtgtaa atggactcga cgcgaccggt catgagacgg agaaggcgac
    16981 ggacggcgtc taccgcaccg acgtcacggt ttcacagggc gaggcgatac gcttcatgca
    17041 gtatatcgac acggcggcgg gttccggcga cgagacgtgt acgctcttcg gcgtacagtc
    17101 gcccggctca tcgaaccaga actacgccgt ctgtctcgag ctcttcggaa ccgaccgcat
    17161 ctccatctcg aaggacgtgt cgaacaacga cacgtcgggc accgtcctcg cgacgtcgac
    17221 cgtcacgtac gagaccggct ggtacgaggt cgagacggca tgggaggcga gcggcaccat
    17281 cgcggtaacg ctctcgaagg acggctccgt cgtcgcgacg acctcggcga acgatagtac
    17341 gtactcctcg ggaggtatgg gcttcacgtt ctggttccag aacggcgggt gggacgacta
    17401 ctccgcgcgc acgctcctta cgacggagcc ttcggtcacg ctcggaagcg agcaggtccc
    17461 cggcggagcc tcatggctcg cggcgctcaa tgcgcccgcg acgggcgtga acacgggcga
    17521 gaacgcgcgt gtcaggttcc tcatcgagaa cagcggcctc cccatcacga atcaggactt
    17581 cgagctcgat tacgcgccga agggtgcgtc accgagctgc gaggcggtct cgtacggaga
    17641 ctacgtgccg gttccgccgt cggcgtcctg cggtacgaac gatctctgta tgcaaacctc
    17701 ggcgaacctc gcgaaccacg aggcgacgac cgacctcctc ggcggtgacg gcaccttcac
    17761 gcccggcgag gccatcgaag acccctcgaa cacgacgaac tccctcgata tcgcccagga
    17821 ctcgtatacg gagctcgaat acgtcgtcac gccgaccgtg aacgtgaccg actcgaacta
    17881 ctgtctccgc gtgacgaacg ccgggagcga cctcgaccag tacacgagga tggcggaact
    17941 ccagctcgtc ttcgcgccga acatcacgtc gctgtccctg aacggcggca tggacatcgc
    18001 gctgaccggc ggttcgacga ctaccgtcta cgcgacgggt acggtgagcg acttaaacgg
    18061 ctacacggac ctcgagttcg cgacgacgac gatattccgt agcggcgtcg gcgaatcgtg
    18121 tacgcccgac aacaacaact gttacgtcgc gtccggtccg cagtgtacct tcacgaactg
    18181 ttcgggaacg tcgtgcgacg tcgagtgtag cgccgatatc tacttccacg ccgacccgac
    18241 ggacgttgga acgttcgcgg gcgagacatg gcgcgcgatg ctcgcgatcg aggacggaag
    18301 cggcctcgtc gcgacggcga ccgcgccatc cgtcgacctc ctcacgcttc gcgccatctc
    18361 ggtcgacgat tcgatcgact acggcgttct cgcggtcaat tccgataccg gctcgtacaa
    18421 cgcgtcgacg accgtcgaga acatcggcaa cgacgcgatc gacgtcgacg tcgagggaac
    18481 cgacctcacc gacggcggct cctccgcgat acccgtctac gagcagaagt tcgcgacgtc
    18541 gaccttcacg tacagttcgt gcgtcttctg cagccagctc tcgacgacga cgtcccacgt
    18601 cgagctcaat ctctcgaagc cgacctcgac ggcaccggcg atcaccgacc acgtcttctg
    18661 gggtatcgcc atcccgttcg gcgtcgcggg aacgcctcac agcggtgtga acaccttcta
    18721 cgcggtgggc gattagcgct gttaataagg aaagtcccac gaatcggggg gtgatacact
    18781 catatatata gtatccatga tgcgttcgtt ctcatcggct cttttagcga ccatggccgt
    18841 gctttggtgc gcgaatctct ttccgatagc cgaggcggcc gatatcacgc ctcccgctat
    18901 ctcgaacgtc gaggtcgcga acgtctcgaa cgacacggtg accatcacgt ggaaaaccga
    18961 tgagaacgct gacgcgacgg tgaactacgg cctccaagag gactacggca tcgtccgcgt
    19021 cgccggggcg acgacgacga cgcactcggt taccctctcg aagctcgagg tgggccgcac
    19081 gtactacttc cgcgtcgtgt cctcggacga ggcggggaac cagggcattt cggcgaacta
    19141 caagatcgtg accgagggca cgccgcagtc cggtgatggg tcgcagtcgt cgacggagac
    19201 ccaatcgaag acgacgcagg agatcgtaca ggagatccag caggtccagt cgccgcagca
    19261 gctccaggag atcatcaacc agacgacgga ggcgctccag ggcattacga aagacctcac
    19321 catcgtcggc ccgccgacgg tcatcgcgga gacgacgagc gcgaccatca agtggacgac
    19381 ggaccgcgaa tcgagctcgg tcgtactctt ctcaccgtcc gactcgtacg tcgccgacca
    19441 gtaccagtac tcgcaggcct cgaccggcgc tgatacgacc gaccacgaag tccgcgtcat
    19501 cggcctcgaa ccgtacaccg agtaccactt caaggtgcag tcgaaggacg cgtacgccat
    19561 aaccggcgag agccgtgatt acaccttccg cacgaaggcg tcgatgcccg atatcaagaa
    19621 tctccatatc gtgaaggtcg aggagaactc ggcgaccctc gcgtgggaca cgacggtccc
    19681 tgcgaaggcg accatcgagt accaggacat gacgaccggt gcgcaaaact ccgtcggacg
    19741 gccgaccctc gcgacgacga accagatgcg catctccgac ctcactctcg ggactcggta
    19801 cgtcgcgttc gtcatcgcgg agaacgccgg cggcgaccgc gtgaagagcc agccgatcac
    19861 cttcgtcacc gtgaaggaca cggccgcccc gatcatctcg aacgtcacga acgagtcgac
    19921 gctcttcccg ggcggcgaat cgcgtatcca gaccatcatc gagtggctca ccgacgaacc
    19981 cgcgcactgt tccttaacgt accaagaggg tatcgcgggc ggcatcgaac cgacgaccat
    20041 cgattcgaag gacagcggct actcggcgaa gcacacggag gtcatcgtcg atttcgctcc
    20101 cgcgaccgtg taccagttct ggctcacgtg cgccgacgag gcggggaact cggtgaagtc
    20161 ggagaactac gtcctcttca cgccgataca ggagaagagc atcatcgaca tcatcatcga
    20221 gaacttctcg agcaccttcg gctgggtcaa gaacatcgga ggcggcggat aatatagagg
    20281 aatcaccatg gctaaaccac tcatcgtgtg caggaatctc gacatcacct acaacaaggg
    20341 gaagtcgaac gagtacaagg cgctacagaa cgtgaacacc gacatctacg aaggggagta
    20401 catcatcctc ttcggcgcgt cgggttcggg gaagtcgacc ctcatgtacg cgatccaggg
    20461 gtccctgccg cccggaggcg gtacgctcct cattcgcggg gacgacatct actcgtaccc
    20521 gccccaggag cgagtgtact tccagcggta catcatgggc atcatcttcc agtcgttcaa
    20581 cctcatcggc tctctcaccg tcctcgataa cgtcgcgctt ccgatgatct tctgcgatgc
    20641 gaacaaggcg agccgcgaac ggcgagctca gatgcttctc gatcgattcg gcgtcggtca
    20701 cgtctcccac aaggtgccat cgatgctctc gggaggtcag cagcagcgcg tttcggtcgc
    20761 ccgctcgatg gtcaacgacc cgcagatcct cctcgccgat gagccgacgg gaaacctcga
    20821 ttccatctcg acccagcagg tgatggacaa gatcgacgag atcaacacgt tcgaccagcg
    20881 caccatcatc atggtgtcgc acaacgcggc gcacctctcg tacgcgcacc gcgtgtacta
    20941 tcttaaggac ggcatcgtcg tccgcgaggt cgtgaacccg cagcgaaagc agatcaagcc
    21001 ggtgaaggaa ggcgagacga tagtgaccga gctcgaacag ctcgcgcgcc tcttcccgta
    21061 cgattcggtc gataccttgc gtgtgaaatc gctgacgaac tacctcaccc agcagtacac
    21121 gttcgaccag ctcattcggc tcgagaaggc ggtcaacctc ctcatcaagg ggaagatccg
    21181 taagcggcag ttcatcgcga ccctgatcaa gaagtacgag gacggcggcg tcggcatcga
    21241 tcgaaagacg gccgatcaca tggccgaggt gtcggtcgcc ctcgtcacgc aagccgacga
    21301 cgtgcgccgg ttccggagcg acaagcaaca gcagtcggag gcggcgttct tcgcccagca
    21361 caagttcgcc gagcgcgtgc gcgagtacct cctcatgcat caccgcgtcc cgctctcgcg
    21421 cgccatggag gcgaacctca cgacgatgat agcggaccgt ctctcaggcg tctcgggcga
    21481 ggaagagttc tatgacgcgc tcggagcgcc ggtcaaggag ggaggactcg gcctcgaacg
    21541 gaaggtcgtc tccgacatct cg
//
LOCUS       DASTSP010000078         5763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_187593, whole genome shotgun sequence.
ACCESSION   DASTSP010000078 DASTSP010000000
VERSION     DASTSP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187593"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="murF"
                     /locus_tag="VFS75_03650"
     CDS             complement(<1..1066)
                     /gene="murF"
                     /locus_tag="VFS75_03650"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU4677782.1"
                     /translation="MKALLKRVIVALLTAEARTLLTRTKPVIIAVTGSVGKTSIKDAI
                     YEVLKDRMHVRKSEKSFNSELGVPLTVLGLRNAWNNPVLWLKNVIDGAMIALHPGTYP
                     KVLVLETGVDRPGDMDRLTAWVKPDIVVLTRFPDVPVHVEYFESPEAVIEEKKKLVTA
                     LRPEGILVFNNDDEKVRKVAEETRQRSIGYSRFSLSPYTASGDTVVYEDGRAAGIEFA
                     LTHLDRAVTVRVNGSVGVQHAYNSAAAAAVASIFDIDIAAVAESLADYTPPPGRMRLI
                     PGIKETLLIDDTYNSSPAASEGSLRTLGELKGVKRRIAVLGDMMELGQFSIREHERIG
                     ALAAKSADILVTIGVRARGFG"
     gene            complement(1074..2789)
                     /locus_tag="VFS75_03655"
     CDS             complement(1074..2789)
                     /locus_tag="VFS75_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU4677783.1"
                     /translation="MRREAVIRIRAITGVVLFILLIIIGRLYVVQIVHGAEYREAGEN
                     QYVHTVRDLFSRGSIYMTTKDGEKVSAATVKTGYLLAIDPSKIADAEKTYEALSSIIP
                     IDRDAFIARASETDRAYAEIAPQVENDEADAIDALNLPGVHLYRNQWRYYPGDSLAAR
                     AIGFVGYDDNALVGKYGLERYYNDTLTRRDEHLAVNFFAEIFSNLGDLIFDSKDTKEG
                     DLVTTIEPSVERALDQALQKSEDMWHGELSGGIIINPQTGEIYALDVDPSFNLNDRSG
                     ADLAMFRDPLVENVYEMGSIIKPLTVAAGLDAGVIVPSSTYYDAGCIELDGKRICNYD
                     GRARGTVPIQQILSQSLNVGVSHIADLLGGERFRHYFYGLKLGSETGIDLPNETYGLV
                     SNLESPRKVEYATASFGQGIAMTPMETVRALSTLANGGKLITPHLVQRVEYGDGTVRD
                     IAYPDGDQVFKKETTEDVTRMLVTVVDEALRNGTVKNPYYSVAAKTGTAQIADPVHGG
                     YYPDKYLHSFFGYFPAYDPKFLIFLYTVEPKGVEYASETLTEPFMDLVHFLINYYELP
                     PDRGQ"
     gene            complement(2796..3113)
                     /locus_tag="VFS75_03660"
     CDS             complement(2796..3113)
                     /locus_tag="VFS75_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677784.1"
                     /translation="MNRRRHTVAAYNGESRLFYAAALSCIIVFAGYVYFLSLSVVHVV
                     MRKEVDGQIAAVNTQVSELETAYIARQQSVSRDVALAKGFVIADKKIFIDKGADTVAF
                     SRN"
     gene            complement(3110..3997)
                     /gene="rsmH"
                     /locus_tag="VFS75_03665"
     CDS             complement(3110..3997)
                     /gene="rsmH"
                     /locus_tag="VFS75_03665"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU4677785.1"
                     /translation="MTHVTVLADEAVDALALTESGAVVDATLGSAGHATRMLAKLGKQ
                     GTLVGIDADETAVAAAKKSLTGDATVHLVTGNFRRLDSILTRLHIDGVDGILADLGWR
                     IEQFTGNGKGFSFQVDEPLLMTLGEPKEYSFTARDIVNEWKEEDIANVIFGYGEERYA
                     RRIARNIVEAREKAPIETTFDLVSVIERSVPGSYRHGRIHPATRTFQALRIAVNDELS
                     ALSEFLETGARVLKKGGRLAVITFHSLEDRIVKHTLRDLAAKGEGTVLTKKPITPSKE
                     ELQANPRSRSAKLRIFEKT"
     gene            complement(4009..4443)
                     /gene="mraZ"
                     /locus_tag="VFS75_03670"
     CDS             complement(4009..4443)
                     /gene="mraZ"
                     /locus_tag="VFS75_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU4677786.1"
                     /translation="MLIGEYTHNIDVKKRLSLPSKWRKELGKKLVVTRGLDNCLFIYP
                     LKEWEKITERIGRLPLGQADTRGFNRFFLSGAVEVEVDTVGRILVPDFLKEFARLTTK
                     VVLAGIHDRVEIWDENRWTEYKKRIEGQADALAEKLGEIGVL"
     gene            complement(4617..>5763)
                     /locus_tag="VFS75_03675"
     CDS             complement(4617..>5763)
                     /locus_tag="VFS75_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU4677787.1"
                     /translation="GDFVEVTGTLFATNTGQRSILAKEWTMLTKSLLPLPDKYHGLQN
                     EEERFRKRYLDLLTHPELRDMFRRKEHFWYVTRSFMKEHGFTEVETPTLELTTGGAEA
                     RPFTTHHNDYDIDVHLRISVGELWQKRLLAAGFDKTFEIGRIFRNEGTSPNHLQEFTN
                     MEFYWAYADFEKGMELVEELYRTIAKEVYGTTSFTYGDYTFDLANDWKRLDYAEEIMR
                     QTGIDVAGASADDMKRKLAELNVEYEGDTRERLLDTLWKHCRKQIAGPAFVTGHPKLV
                     SPLAKEIEGKDATQRFQPVIAGTEVGNGYSELNNPLEQAARFAVQQELLVAGDEEAMM
                     ADHEFVEMLEHGMPPACGFGFGERLFAILEGKPVRETQLFPLLRPKA"
BASE COUNT         1014 a         1709 c         1780 g         1260 t
ORIGIN      
        1 tcccgaaccc gcgtgcgcgt acgccgatcg tgacgaggat gtcggccgac ttcgcggcga
       61 gggcgccgat gcgctcatgt tcgcgtatcg agaactgtcc gagctccatc atgtcgccga
      121 ggacggcgat gcgtcgcttc acgcccttta gttcaccgag cgtgcggagc gagccctcgg
      181 aggccgccgg cgaggaattg taggtatcat cgatgagaag ggtctccttg atcccgggaa
      241 tgagacgcat gcggccgggc ggcggcgtgt agtcagcgag cgattccgcg accgcggcga
      301 tgtcgatatc gaagatggac gcgacggccg ccgctgcggc cgaattgtac gcgtgctgaa
      361 ctccgacgga gccgttcacc cggacggtga ccgcgcggtc gaggtgcgta agagcgaact
      421 cgatgcccgc ggcccggccg tcctcgtaga cgacggtgtc gcccgaggcc gtgtacggcg
      481 agagcgagaa gcgtgagtag ccgatcgagc gttgtcgcgt ctcttcggcg acctttcgca
      541 ctttctcgtc gtcgttgttg aagacgagaa taccctcggg tcgaagcgcg gtgacgagtt
      601 tcttcttctc ctcgatgacc gcctcgggcg attcgaagta ctcgacgtgg acgggcacgt
      661 ctgggaagcg cgtcaggacg acgatgtccg gcttcaccca cgcggtgagt cggtccatgt
      721 cgcctggcct atcgacgccg gtctcgagga cgaggacctt cgggtacgta ccgggatgga
      781 gcgcgatcat cgcgccgtcg atgacgttct tcagccacag cacggggttg ttccaagcgt
      841 tccggaggcc gaggacggta agcggcacgc cgagctccga attgaagctc ttctcgctct
      901 ttcgcacgtg catacggtcc ttgagtacct cgtagatcgc gtccttgatg gaggtcttcc
      961 cgacgctccc ggtgaccgcg atgatgaccg gtttcgtacg cgtaaggagc gtccgcgcct
     1021 cggcggtgag gagcgcgacg atgactcgct tcaagagggc tttcatagat gggttactgg
     1081 cctcggtcgg gcggtaattc gtaatagttt atgaggaaat ggacgaggtc catgaagggc
     1141 tcggtgaggg tctccgacgc gtactcgacg cccttcggtt caacagtata caggaaaatg
     1201 aggaatttcg gatcgtacgc cgggaagtat ccgaagaacg agtggaggta cttatcggga
     1261 tagtagccgc cgtgtacggg gtcggcgatc tgggcggttc cggtcttcgc ggcgaccgaa
     1321 tagtacgggt tcttcacggt cccgttccga agcgcctcgt cgacgacggt gacgagcatg
     1381 cgagtgacgt cctcggtggt ctccttcttg aagacctggt cgccatcggg gtacgcgatg
     1441 tcgcgtaccg tcccgtcgcc gtactcgacg cgctgcacga gatgcggcgt gatgagtttc
     1501 cctccgttcg cgagggtcga gagcgcgcga acggtctcca tgggcgtcat ggcgataccc
     1561 tgtccgaagg acgcggtcgc gtactcgact tttcgcgggc tctcgaggtt cgatacgagc
     1621 ccgtacgtct cattgggaag gtcgatgccc gtctccgaac cgagtttgag tccgtagaag
     1681 tagtggcgga agcgctcccc gccgagaagg tcggcgatat gggagacacc gacgttgagg
     1741 gactggctca agatctgctg aatggggacg gtgccgcgcg cgcgtccgtc gtagttacag
     1801 atgcgcttac cgtcgagttc gatacacccc gcatcgtagt acgtcgaact cgggacgatg
     1861 acgcccgcat cgagtcccgc cgcgacggtg aggggtttga tgatcgatcc catctcgtag
     1921 acgttctcga ccagggggtc acggaacatc gcaaggtcgg ctcccgagcg gtcgttcaag
     1981 ttaaacgagg ggtcgacgtc gagcgcgtat atctcgccgg tctgcggatt gatgatgata
     2041 ccgccggaga gctcgccgtg ccacatgtcc tccgattttt ggagcgcctg gtcgagcgcc
     2101 cgctcgaccg acggctcgat ggtcgtcacg aggtcgcctt ccttcgtgtc cttcgaatcg
     2161 aagatgaggt cgccgaggtt actgaatatc tcggcgaaga aattgacggc gagatgttcg
     2221 tcgcgacgcg tgagcgtatc gttgtagtag cgttcgagac cgtacttccc gacgagcgcg
     2281 ttgtcgtcgt atccgacgaa gcctatcgcg cgcgcggcga gcgaatcacc cgggtagtat
     2341 cgccactgat ttcggtagag gtgtacgccg gggaggttga gggcgtctat cgcgtccgcc
     2401 tcgtcgttct cgacctgtgg tgcgatctcc gcgtaggcgc ggtccgtctc ggacgcgcgc
     2461 gcgatgaacg cgtcacggtc gatggggatg atgctcgata gcgcttcgta cgtcttctct
     2521 gcatccgcta tcttcgatgg gtcgatggcg aggaggtacc cggtcttcac ggtcgccgcg
     2581 gacaccttct ccccgtcctt cgtcgtcatg tagatactgc cgcgactgaa aaggtcgcgg
     2641 acggtgtgca cgtactggtt ctcgcccgcc tcgcggtatt cggcgccatg gacgatctgg
     2701 acgacgtaga gtcgtccgat gatgatgagt agtatgaaaa ggacgactcc ggtgatggcg
     2761 cgtatccgaa tgactgcttc ccgcctcatg tgaacttaat ttcgtgagaa cgcgacggtg
     2821 tcggctccct tgtcgatgaa tatcttctta tccgcgatga cgaacccctt cgcgagcgcc
     2881 acgtcacggg acaccgattg ctggcgagcg atgtatgccg tctcgagctc gctcacctgt
     2941 gtgttcaccg ccgctatctg gccgtcgacc tccttccgca tgacgacgtg gacgacggag
     3001 aggctaagaa agtacacata gccggcgaat acgatgatgc acgagagggc tgccgcgtaa
     3061 aacaggcgtg attctccgtt gtatgccgct acagtatgtc gtcgtcgatt catgtttttt
     3121 cgaaaattcg tagtttagcg cttcgtgatc gtgggtttgc ctgtaattcc tctttcgacg
     3181 gcgttatggg cttcttcgtg aggacggtgc cttcgccctt cgcggcgaga tcgcggagcg
     3241 tgtgcttcac gatgcggtcc tcgaggctgt ggaaggtgat gaccgcgagc cgtcctccct
     3301 tcttgaggac acgggctccc gtttcgagga attcggagag tgccgagagc tcgtcgttca
     3361 ccgcgatgcg gagcgcctgg aaggtccgtg tcgctgggtg gatgcgcccg tgccggtagc
     3421 tcccgggcac cgagcgttcg atgaccgaaa cgaggtcgaa cgtcgtctcg atgggcgcct
     3481 tctcgcgcgc ctcgacgatg ttccgcgcga tgcgccgcgc gtaccgctct tcgccgtacc
     3541 cgaagatgac gttcgcgatg tcctcttcct tccactcgtt cacgatgtct cgcgcggtga
     3601 aggaatactc cttaggttcg ccgagggtca tgaggagcgg ttcatcgacc tggaacgaga
     3661 atcccttccc gttccccgtg aactgctcga tgcgccaacc gaggtccgcg agtatgccgt
     3721 ctacgccgtc aatgtgcaat cttgttaaaa tcgagtcgag ccgtctgaaa ttcccggtga
     3781 cgaggtgtac cgtcgcatcg cccgtgagcg acttcttcgc cgcggcgacc gccgtctcgt
     3841 ccgcgtcgat gccgacgagc gtcccttgct tcccgagctt cgcgagcatg cgcgtcgcgt
     3901 ggccggcgga cccgagggtc gcatcgacga ccgccccgct ctctgtaagc gcgagcgcgt
     3961 cgaccgcctc atcggctagt acggtaacgt gagtcatatg tcgcttcgct agaggacgcc
     4021 gatctcaccg agcttctcgg cgagggcgtc agcctgtccc tcgatgcgct tcttgtactc
     4081 cgtccaccga ttctcatccc atatttctac acgatcatgg atacccgcga gcacgacctt
     4141 cgtcgtgagc ctcgcgaatt ccttcaagaa atcaggaacg agaattcgtc cgacagtgtc
     4201 tacctccacc tccaccgctc ccgagaggaa gaatcgattg aatcctctcg tatccgcttg
     4261 accgagcggg agccgaccta tcctttcggt gatcttctcc cactctttca agggatatat
     4321 aaagagacag ttatcgagac cgcgagtcac gaccagcttc ttgccaagtt cctttctcca
     4381 tttggagggt aaggacagcc gtttcttgac gtcgatgttg tgcgtgtact ccccaatcaa
     4441 catgtgtttg attgcccgtc tcgatactga taactattct catagtttac cactatctcc
     4501 caccacgggt ccattatata ccactctctc ccaccccctg ccacagttat acacaagacc
     4561 tcgtgaagga ggcatgaaaa aacccgggct ccccgggttc ttgttcggtc gtacagttat
     4621 gccttcggtc ttaggagtgg gaagagctgt gtctcgcgga cgggcttccc ctcgaggatg
     4681 gcgaagaggc gctctccgaa tccgaagccg catgcgggcg gcatgccgtg ttcgagcatc
     4741 tcgacgaact catggtcggc catcatcgcc tcctcgtcgc ccgcgacgag aagttcctgc
     4801 tgaacggcga agcgcgccgc ctgctcgagg ggattgttga gctccgagta gccgttcccg
     4861 acctccgtgc ccgcgataac gggctggaag cgttgcgtcg cgtccttgcc ctctatctcc
     4921 ttcgccaagg gcgaaacgag tttcgggtga cccgtcacga acgcggggcc cgcgatctgc
     4981 ttcctgcagt gcttccagag cgtatcgagg agccgctcac gtgtatcacc ctcgtactct
     5041 acgttcagtt cggcgagctt ccgcttcatg tcgtcggctg acgcaccagc gacgtcgatg
     5101 ccggtctgcc gcatgatctc ctcggcgtag tcgagacgct tccagtcgtt cgcgaggtcg
     5161 aacgtatagt cgccgtacgt gaacgacgtc gtaccgtaga cctccttcgc gatcgtgcgg
     5221 tagagctcct cgacgagttc catgcccttt tcaaaatctg cgtacgccca gtagaactcc
     5281 atgttcgtga actcctggag gtggttcggg ctcgtaccct cgtttcggaa gatgcggccg
     5341 atctcgaacg tcttatcgaa gccggcggcg aggaggcgct tctgccagag ctcgccgacc
     5401 gagatgcgga ggtgtacgtc gatgtcgtag tcgttgtgat gcgtcgtgaa gggacgcgcc
     5461 tccgcgccgc ccgtcgtgag ctcgagggtc ggcgtctcga cctcggtgaa gccgtgctcc
     5521 ttcatgaacg agcgcgtcac gtaccaaaaa tgttcctttc gcctgaacat atcgcgaagc
     5581 tcgggatgcg tgaggaggtc gaggtagcgc ttccggaatc gctcctcctc gttctgaaga
     5641 ccgtggtact tgtcgggaag cggaagaagg ctcttcgtaa gcatcgtcca ctctttggcg
     5701 aggatggaac gttggccggt attcgtcgca aagagcgtcc ccgtcacctc gacgaaatca
     5761 ccc
//
LOCUS       DASTSP010000079         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1306747, whole genome shotgun sequence.
ACCESSION   DASTSP010000079 DASTSP010000000
VERSION     DASTSP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306747"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFS75_03680"
     CDS             complement(<1..538)
                     /locus_tag="VFS75_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU4677788.1"
                     /translation="MAPRTIETDCCIAGGGPAGIMLGYLLARAGIRVTVLEMWPDFFR
                     DFRGDTIHPSTMNILRELGLLDEFLKLPHSEMAKMTFRAGERSVTIADFERLDVETKY
                     IAFIPQWDFLNFLATKAKEFPTFELRMATKAADLVTEGGRVVSVRAEDADGALEIRAR
                     LVIGADGRHSIVREKGGFE"
     gene            complement(577..945)
                     /locus_tag="VFS75_03685"
     CDS             complement(577..945)
                     /locus_tag="VFS75_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677789.1"
                     /translation="MGLKELLKISGIPVLLASLCCLSPIVLVLLGVSTVSFASTLSDV
                     LYADFKWAFRLVGLLALALSILYYLRRTRGICTLDDARRRRNEVLNYVAFSLIACVLG
                     YLFFLYIVVHYVGLWLALWV"
     gene            complement(947..2002)
                     /gene="msrB"
                     /locus_tag="VFS75_03690"
     CDS             complement(947..2002)
                     /gene="msrB"
                     /locus_tag="VFS75_03690"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4677790.1"
                     /translation="MRSEHMTTATTSTALFADGCFWCVEHDMEAVQGVIDVVSGYAGG
                     TSESPTYENYAEGGHREVVEVTYDPSKVTYANLVEHILKHGDPTDADGSFYDRGPQYT
                     PAIFYDTDDEKVEAEAIIKAADALKVFPKPITITVSPRPRFWPAEEYHQDYAKKNPLR
                     YMYYRAASGRDSFIKKYWGESADSFTISSSISAPKTTAFNAHSWNGYVKPSAKELKAR
                     LTPLQYEVAEEGATEPPFKNAYDENHEKGVYVDVVSGEPLFLSTDKYESGTGWPSFVK
                     PITPDAVTLHEDDTLFTARTEVRSAHANSHLGHVFDDGPMDRGGKRYCMNSAALRFIA
                     YDDLDREGYGYTKSYFD"
     gene            complement(2243..2599)
                     /locus_tag="VFS75_03695"
     CDS             complement(2243..2599)
                     /locus_tag="VFS75_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677791.1"
                     /translation="MNKNHWYIGIALVLTFLLLTLADLVPFWMPMMWEMTALLIAVVL
                     LLAWAGFVLKEGARDEREALLTLRSGRVAYLAGLGVLMVALVVQCLGPAPTDPWVAIT
                     LSVMVLAKLFTRAREE"
     gene            complement(2592..2801)
                     /locus_tag="VFS75_03700"
     CDS             complement(2592..2801)
                     /locus_tag="VFS75_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4677792.1"
                     /translation="MEAFVKNKVNEYRTKAGLTQEELATRVGVTRQTIIAIERGNYTP
                     SVLLAIRLAEVFSVPVESLFSIAYE"
     gene            2910..3167
                     /locus_tag="VFS75_03705"
     CDS             2910..3167
                     /locus_tag="VFS75_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677793.1"
                     /translation="MKRVAFATFGLLLIPLLAMVFTDSVNWEINDFIVMGVMLFTAGM
                     LIELALKKFSTYRMVAVGVVVLAFLWLWAELAVGVFTNWGS"
     gene            complement(3221..>3637)
                     /locus_tag="VFS75_03710"
     CDS             complement(3221..>3637)
                     /locus_tag="VFS75_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4677794.1"
                     /translation="ANTAEIRYLRSPEGLNPSQMEGFFVGWEKRPTAPTLLKILSKSD
                     HVAIAMHGDQVVGFATANSDHVISAHIPLVEVLPEYQGKGIGKTLVELLLEDFKDIYM
                     IDLMCEEDVEGFYEKFGFHKVPAMMLRHYDKQLGEG"
BASE COUNT          749 a         1046 c         1061 g          781 t
ORIGIN      
        1 cctcgaaccc tcccttttcg cgaacgatcg aatggcggcc atcagccccg atgacgagcc
       61 gtgcgcgtat ttcgagcgca ccgtccgcat cttccgcccg tacgctgacg acgcgtcctc
      121 cctccgtcac gaggtccgcc gccttcgtcg ccatgcggag ctcgaacgtc gggaactcct
      181 tcgccttcgt cgcgagaaag ttgagaaagt cccactgcgg gatgaacgcg atgtacttcg
      241 tctcgacgtc gaggcgctcg aaatcggcga tggtcacgct ccgctcgccg gcgcggaacg
      301 tcatcttcgc catctcgcta tggggcagct tgaggaactc atcgaggagt ccgagttcgc
      361 gaaggatgtt catcgtcgat gggtgtatcg tgtcgcctcg aaagtccctg aagaagtcag
      421 gccacatctc gagaacggtg acgcgtatac ccgctcgcgc gaggagatac ccgagcatga
      481 tgcccgcggg cccgccaccc gcgatacaac agtccgtttc tatggtgcgt ggtgccattt
      541 cgtcagtata taccccgcga acgatcgaac cgaccgttac acccagaggg caagccaaag
      601 gccgacgtag tgaacgacaa tatagagaaa gaagaggtag ccaagaacgc aggcaatgag
      661 agagaacgcg acgtagttaa gcacttcgtt ccggcgtcgc cgcgcatcgt cgagggtgca
      721 aataccacgt gtccttctca agtagtacag gatggagagg gcgagcgcga gcagaccgac
      781 gagccgaaac gcccacttga agtccgcata gagcacgtcg gagagggtcg atgcgaagct
      841 caccgtcgac acgccgagga gcacgagaac gatcggggaa agacaacaga gcgacgcgag
      901 aaggaccggg atgccgctta tctttaggag ttccttaaga cccatgtcag tcgaaatacg
      961 atttggtgta cccgtaccct tccctgtcga gatcatcgta ggcgatgaat cggagcgctg
     1021 ccgagttcat acaatagcgc ttccctcctc ggtccatcgg tccgtcgtcg aagacgtgcc
     1081 cgaggtgcga gttggcgtgc gcggagcgca cctcggtacg cgccgtaaaa agcgtgtcgt
     1141 cctcgtggag cgttaccgca tcgggcgtga tgggcttcac gaacgatggc cagcccgtcc
     1201 ccgactcgta cttatcggtc gagaggaaaa gcggttcgcc ggagacgacg tcgacgtaca
     1261 cacccttctc atggttctca tcgtacgcgt tcttgaaggg tggttcggtg gcaccctcct
     1321 cggccacctc gtactgaagc ggcgtgagcc gcgccttgag ctctttcgct gacggcttca
     1381 cgtatccatt ccatgaatgc gcgttgaacg cagtcgtctt cggagcgctg atgcttgacg
     1441 agatggtgaa cgagtctgcc gactcacccc agtacttttt tatgaacgaa tcgcgccctg
     1501 aggcggcacg gtagtacatg tagcgaagcg ggttcttctt cgcgtagtcc tgatggtact
     1561 cctcggcggg ccagaatcgc ggccgaggcg agaccgttat cgtaatgggt tttgggaaga
     1621 ctttgagcgc gtctgccgct ttgatgatgg cctcggcctc gaccttttcg tcatcggtat
     1681 cgtagaagat agccggcgtg tactgcggac cgcggtcgta aaacgagccg tccgcgtcgg
     1741 tcgggtcccc gtgcttcaaa atatgctcga cgaggttcgc gtatgtgacc ttggatgggt
     1801 cgtacgtcac ctcgacgacc tcgcgatgac ctccctccgc gtagttctcg tacgtcggcg
     1861 actcgctcgt ccctcccgca tagcccgata cgacgtcgat gacgccctgc acggcctcca
     1921 tgtcgtgctc tacgcaccaa aagcagccgt ccgcgaagag ggcggtcgag gtcgtcgcgg
     1981 ttgtcatatg ttctgatcgc atggtagttt cgctcgtccc cgtactggtc gtttcgttca
     2041 taggaatggg tatacggcgc tccgccgaac gtccacgctc cgctagtaat gaggaaacga
     2101 tccgcggcat cccgctcgtc agatagagaa acgttccgag gaccgcgacc gccgccacgc
     2161 cgatgatgac gttcttgagc atggtcttag tatatgacca tgaaggagag atgtaaagtt
     2221 acctttacat ctgccatgtg gactactctt cgcgtgctcg cgtaaagagc ttcgcgagga
     2281 ccattacgga gagcgtgatg gcgacccacg ggtcggtagg cgcgggtcca aggcactgaa
     2341 cgacgagcgc gaccatgagg acgccgaggc ccgcgaggta cgcgaccctc cccgagcgga
     2401 gcgtgaggag cgcctctcgt tcgtcgcggg ccccttcctt gaggacgaat ccggcccacg
     2461 cgaggaggag gacgactgcg atgaggagcg cggtcatttc ccacatcatc ggcatccaaa
     2521 acggaacgag gtccgcgagg gtgaggagga gaaacgtgag cacgagagcg atgccgatgt
     2581 accagtgatt tttattcata cgcgatgcta aacaaggatt cgacgggcac ggagaacacc
     2641 tcggcgaggc gaatcgcgag gagtaccgac ggcgtgtagt taccgcgctc gatcgcgatg
     2701 atggtctggc gagtcacgcc gaccctggtc gcgagctcct cctgcgtgag ccccgccttt
     2761 gtgcgatact cgttcacctt gtttttcacg aaggcctcca tttgataatg gcattagtgt
     2821 agcacgcgac cccgttcgcg taaccgagcg gcgtattcgc ggttatagag tatgtattcg
     2881 ataaacatgt gatcatggta cgaaacagca tgaaaagagt ggccttcgcg acgttcggac
     2941 ttctccttat cccgcttctc gctatggtct tcacggactc ggtgaactgg gagatcaatg
     3001 atttcatcgt catgggcgtc atgctcttca cggccggcat gctcatcgag cttgcgctca
     3061 agaaattcag cacataccgc atggtcgcgg tcggggtcgt cgttctcgcg ttcctctggc
     3121 tctgggccga gctcgcggtc ggcgtcttca cgaattgggg tagttaaacg atcgactctc
     3181 gctaaatggg agaaccacct gcttttattg tatctgttcg ctatccttct cctagttgct
     3241 tgtcgtaatg tcgcagcatc atggcaggta ctttatgaaa accaaatttc tcgtaaaaac
     3301 cctcgacatc ctcttcgcac atgaggtcta tcatatagat gtccttaaag tcctcgagca
     3361 ggagctcgac gagagtcttg ccgattccct tgccttgata ttcggggaga acctcgacga
     3421 gcggtatgtg cgctgatatc acatggtcgc tattcgccgt cgcaaaaccg acgacctggt
     3481 ctccatgcat cgcgatcgcg acgtgatcgc tcttggagag aatcttaaga agcgtcggtg
     3541 cagtcggtcg tttttcccat ccgacgaaga acccttccat ctgtgacggg ttcaaaccct
     3601 cgggagaccg aaggtagcgt atttccgcag tatttgc
//
LOCUS       DASTSP010000080         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1756182, whole genome shotgun sequence.
ACCESSION   DASTSP010000080 DASTSP010000000
VERSION     DASTSP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756182"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFS75_03715"
     CDS             complement(<1..431)
                     /locus_tag="VFS75_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677795.1"
                     /translation="MDTETVTVFEPKLHALVDERTMRRLMDLYLEEHSANRAFIEIAT
                     VLGERRHYTAVGIMLLMVETYVFTANVAWALLLPLTAVLFYLAILDRYAKGPAFWYIR
                     EKARFMIPSYVHEADSRFGIEHKNKSWEIMELCRNGELLAGV"
     gene            complement(502..1185)
                     /locus_tag="VFS75_03720"
     CDS             complement(502..1185)
                     /locus_tag="VFS75_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4677796.1"
                     /translation="MPTIIEANDIHRTFGTGELETRVLKGITLTVSAGDFVAIMGKSG
                     AGKSTLMYQLSVLDHPTTGRVTVDGVNVASLSESEQTRFRLNTLGYVFQDYALVPDLS
                     AEENIMVPLLMRGLSWEEAKTKAREALTGVGLPGKERNLPAQLSGGEQQRVSVARAIA
                     GGPKIIFADEPTANLDSVSGNAIIELLRDLNRRGQTIVMVTHEEEYTKYCNRIIDMED
                     GRITGERRP"
     gene            complement(1215..2462)
                     /locus_tag="VFS75_03725"
     CDS             complement(1215..2462)
                     /locus_tag="VFS75_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4677797.1"
                     /translation="MRTLRIGLLLGIRQIQRASLSTTLLIIFVMMFTFLNLIAVSGVL
                     VGIIVGAERAVRENAYGDLTISPKAGEDAILETPTIVRELGRYPEITAYSVRYSGVGK
                     LEANFKERRDLKKDADVANVKVTGIDVEAENRLTHLSDYVVEGEYLDPNEEGYILIGA
                     YYLSQYAELYGDIFETVEDVHPGDTVRLSVGDVSKEFTVKGVVQSKVDDVNLNTYISS
                     REFRRIFGRIDSNADLIVTRMADPAQNSSVKASLVRTGLDRYAKIQTFEEGVPKFLAD
                     IKRTFNLLGTFIGSIGVIVASITIFIIIFINALARKRHIGILKGIGIDEKVIELAYVM
                     QAAFYALAGSLLGALLTYGVFIPYTDRHPIQFPFSEGVLVADPLSTFYKFLVLFAVTL
                     IAGYLPAKLITRENTLNAILGRK"
     gene            complement(2465..3976)
                     /locus_tag="VFS75_03730"
     CDS             complement(2465..3976)
                     /locus_tag="VFS75_03730"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU4677798.1"
                     /translation="MSRLKTLFRNRLVLIGAVLLVVAIVAFALKSKNDAPTFTTDTVS
                     VGAVKNVVSVSGTLDAADSVALSFPVTGTLASVSVTEGDTVTKGQELASLSHADLSAQ
                     YSDAYAALLIAEADRDELISGLRPEELAVTKTTAEIAREDLERVTKEQNDRVENAYRT
                     LLSTDLEARPVDPENDDIAPTVSGTYACDKEGTYTLTMFRSASLSGYSYRLSGLESGT
                     FTAYANAAAPLGSCGLYVQFADGESYGDSTWTIDIPNKTGASYVSDLNAYNLAKTERA
                     NEIAAATQKLTLATENASLDTATPRQEARTRADAKVLQAQARLQAVSANIDERVLRAP
                     FSGTVTRVDAVVGETAGAGPIVTMVGDGSFELTALIPEIDVTKIQVGSKADVSFDARP
                     DESFGATVVFVSPLAKQVEGVSYFEAKLALDEELDWLKSGLSADIDIVTGEKDGVLRV
                     PRRYLIGENGSYSLLVPAGKGTRTVPVTVTFIGNDGFAAVEGIEAGTTVVAPE"
BASE COUNT          698 a         1226 c         1311 g          820 t
ORIGIN      
        1 acgccggcga ggagctcacc gttccggcac aattccatga tctcccacga tttgttcttg
       61 tgctctatgc cgaaacgact gtctgcctcg tgcacgtagc ttggaatcat gaagcgcgcc
      121 ttctcgcgga tgtaccagaa cgccggcccc ttcgcgtagc ggtcgagaat ggcgaggtag
      181 aagaggacgg ccgtaagcgg aaggaggagc gcccacgcga cgttcgccgt gaagacgtac
      241 gtttcgacca tgagaagcat gatgccgacg gccgtgtagt gtcgcctctc gccgaggacc
      301 gtcgcgatct cgatgaacgc gcgattcgcg gagtgctctt cgaggtagag atccatgagc
      361 cgtcgcatcg tcctctcgtc tacgagcgca tgcaacttcg gttcgaaaac ggtgaccgtt
      421 tcggtatcca tgtggcctcc caagccggat tgtctcgcgg catcatgcca cactttcggg
      481 ctatcgtaca ggctcgttct tctacgggcg gcgttctccg gtgatgcgcc cgtcctccat
      541 atcgatgatg cggttgcaat acttggtgta ctcctcctcg tgggtcacca tgacgatcgt
      601 ctgacctcgt cggttcaggt cgcggaggag ttcgatgatc gcgttccccg acacgctgtc
      661 gaggttcgcg gtcggttcgt cggcgaagat gatcttcggt ccgcccgcga tcgcgcgggc
      721 gaccgagacg cgttgctgtt cgccgcccga gagttgggcg gggaggttcc gttctttgcc
      781 ggggagcccg acgccagtga gcgcctcgcg agccttcgtc ttcgcctctt cccatgagag
      841 gccgcgcatg aggagcggca ccatgatgtt ctcctcggcg gaaagatcgg ggacgagcgc
      901 gtagtcctgg aagacgtagc cgagcgtatt gaggcggaaa cgcgtctgct cggactccga
      961 gagcgacgcg acgttcacgc cgtcgacggt gacgcgtccc gtcgtcgggt ggtcgaggac
     1021 cgagagctgg tacatgaggg tcgatttgcc ggctcccgat ttgcccatga tcgcgacgaa
     1081 atcgcccgcg ctcacggtga gcgtgatgcc tttgaggaca cgcgtctcga gctctcccgt
     1141 gccgaacgtt cgatggatgt cgtttgcttc gatgatggta ggcatagggt ttgtgaccat
     1201 actagcgtct catttcattt tctgccgagg atcgcgttca gcgtgttctc gcgggtgatg
     1261 agcttcgcgg ggaggtagcc cgcgatgagc gtgaccgcga agaggacgag gaacttgtag
     1321 aaggtcgaaa gggggtcggc gacgaggacg ccctcgctga acgggaactg gatggggtgg
     1381 cggtcggtgt acgggataaa gacgccgtac gtgaggagcg cgccgaggag cgaccccgcg
     1441 agcgcgtaga acgccgcctg catgacgtac gcgagctcga tgaccttctc gtcgatgccg
     1501 atgcccttga ggatgccgat gtgccgcttt cgcgcgagcg cgttaatgaa aatgatgatg
     1561 aatatcgtga tcgacgcgac gatgacgccg atcgagccga tgaacgtacc gaggagattg
     1621 aaggtccgct tgatgtcggc gaggaacttc ggcacacctt cctcgaacgt ctgtatcttc
     1681 gcgtaccggt cgagcccggt gcgtacgagg gacgccttca ccgaggagtt ctgggcaggg
     1741 tccgccattc gcgtcacgat gaggtctgcg ttcgaatcga tccggccgaa gatgcgccga
     1801 aactcgcggg aggagatata cgtgttcaaa ttgacgtcgt cgaccttcga ctgcacgact
     1861 cccttcaccg tgaactcctt cgagacgtcg ccgaccgaaa gccggaccgt gtctcccggg
     1921 tgcacgtcct cgaccgtctc gaatatgtcg ccgtagagct cggcatactg tgagaggtag
     1981 tacgcgccga tgaggatgta tccctcctcg ttcgggtcga gatactcgcc ctcgacgacg
     2041 tagtcactaa ggtgcgtgag cctattctcc gcctcgacgt cgatgcccgt caccttaacg
     2101 ttcgcgacgt ccgcgtcctt cttgaggtcg cggcgttcct tgaagttcgc ctcgagcttg
     2161 ccgacgccgc tgtagcggac cgagtacgcg gtgatctcgg ggtagcgccc aagctcgcgg
     2221 acgatggtcg gggtctcgag tatcgcgtcc tcgcccgcct tcggcgagat ggtgaggtcg
     2281 ccgtacgcgt tctctcgtac ggcccgctcc gcaccgacga tgatgccgac caagacgccc
     2341 gagacggcga tgaggttgag gaacgtgaac atcatcacga agatgatgag gagcgtcgtc
     2401 gagaggctcg cgcgctggat ctgtctgatc ccgaggagga gcccgatacg gagcgtgcgc
     2461 atacctactc gggggcgacg accgtcgttc cggcctcgat gccttcgacc gcggcaaagc
     2521 catcgttccc gatgaaggtc acggtgacgg gaaccgtccg cgtgcctttc cccgccggca
     2581 cgaggagact gtacgaaccg ttttcgccga tgaggtagcg gcgcggaacg cgaaggacgc
     2641 cgtctttctc gcccgtcacg atgtcgatat ccgccgagag gccgctcttc agccaatcga
     2701 gctcctcatc gagcgcgagc ttcgcctcga agtacgaaac gccctcgacc tgcttcgcga
     2761 gcggcgagac gaacacgacg gtcgcaccga agctctcgtc gggccgcgca tcgaaggata
     2821 cgtccgcctt gcttcccact tggatcttcg tcacatcgat ctcgggaatg agcgccgtga
     2881 gctcgaacga accgtcaccg accatggtga cgatgggccc cgcacccgcg gtctcgccca
     2941 cgaccgcgtc gacgcgcgtg accgtcccgg agaacggagc gcgaagaacc cgctcgtcga
     3001 tgttcgcgct caccgcttgg agccgagcct gtgcctggag gactttcgcg tcagctcgcg
     3061 tacgcgcctc ctgtcgcgga gtggcggtgt cgagggacgc gttctctgtc gcgagcgtga
     3121 gcttctgcgt cgccgccgcg atctcgttcg cgcgctccgt cttcgcgagg ttgtatgcgt
     3181 tcaggtcgct cacgtacgac gcgccggtct tattcgggat gtcgatggtc cacgtgctgt
     3241 caccgtagct ctcgccgtcc gcgaattgca cgtagagtcc gcatgaaccc agtggcgccg
     3301 ccgcgttcgc gtacgcggtg aacgtgccgg actcgaggcc gcttagccgg tacgagtatc
     3361 ccgagagcga tgccgaccgg aacatggtga gcgtatacgt cccctccttg tcgcatgcgt
     3421 acgtaccgct caccgtcggc gcgatgtcat cgttctccgg atcgaccgga cgggcttcga
     3481 ggtcggtcga gaggagcgtg cggtacgcgt tctcgacacg gtcgttctgt tccttcgtca
     3541 cgcgttcaag gtcctcgcgc gcgatctctg cggtcgtctt cgtcacggcg agctcctctg
     3601 ggcggaggcc cgagatgagc tcatcgcgat cggcctctgc gatgaggagc gccgcgtacg
     3661 cgtcgctgta ctgtgccgag agatccgcgt gcgagagcga cgcgagctcc tgtcctttcg
     3721 tgacggtgtc accttcggtc accgagacgc ttgcgagcgt gccggtcacg gggaaggaga
     3781 gcgcgacgct gtccgccgca tcgagcgttc cagagacgga tacgacgttc ttgaccgctc
     3841 ccacggaaac cgtatcggtc gtgaaggtcg gagcgtcgtt cttggatttg agggcgaacg
     3901 cgacgatcgc gacgacaagg agcaccgctc cgatgaggac gagcctgttt cgaaagagtg
     3961 ttttgagccg cgacatcgct aaagtatagc gtggattgcg gtgagtgtct ctctacgata
     4021 gagtggtagc gcacttttcc gcccctagct cagct
//
LOCUS       DASTSP010000081         8587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_640247, whole genome shotgun sequence.
ACCESSION   DASTSP010000081 DASTSP010000000
VERSION     DASTSP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640247"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFS75_03735"
     CDS             <1..363
                     /locus_tag="VFS75_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU4677799.1"
                     /translation="HTLAQERLAKELVDDGADLVIGHHPHVVEDVALYRGKPVFYSLG
                     NFVFDQYFSTDVMEGLGVTLTLENEYADYALTGYATMESRSAPYVMSTTSSSALYARV
                     LQNVPGAELERGTFSVPR"
     gene            417..965
                     /locus_tag="VFS75_03740"
     CDS             417..965
                     /locus_tag="VFS75_03740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4677800.1"
                     /translation="MNFFNKTEVVGLFASVAVMALALAFVRFKTDIFAEAPLLRGDSQ
                     SAIVAVSSATGDATTSALRGALEEASTGKGDLVKLVVDDIVIGTGPKVKNGDTVTVNY
                     EGRTQSGVTFDSSYERGTPFTFTVGAGKVIEGWEKGLVGMQVGGKRILVIPADMAYGN
                     RQVGVIPANSPLVFSIELLKIE"
     gene            969..2162
                     /gene="tgt"
                     /locus_tag="VFS75_03745"
     CDS             969..2162
                     /gene="tgt"
                     /locus_tag="VFS75_03745"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU4677801.1"
                     /translation="MTSPISFEILKRKPGTLARAGVLRTPHGDIETPAFIAVGTKGTV
                     KSLKPDDLERYVGNEMTLANTYHLYLQPGADVVKEAGGLNRFTGWKTPTITDSGGFQI
                     FSLGAAFGKGVTKFATAERGDESAGIRPYSKELATSHGKLCVIDDEGVTFTSHLDGSM
                     HRFTAERSIEIQHDIGADIIIAFDECTSPNAPHDYQKEAMDRTHRWAKRSILAHKRDY
                     DALKRQGIFGVVQGGRYEDLRRESAATLAAMDFDGFGIGGSFSKADLGESLRVVNEVL
                     PEDKPRHLLGIGEPEDLRAGVLMGCDLFDCVQPTRLGRTGTIYVHGRDGLDKLNLTKS
                     EYQRDFGKPDPACECHVCSNYTRAYLAHLFRANEMLGPHLASLHNLHAIVAYTKSLRA
                     EILAS"
     gene            2213..4240
                     /gene="uvrB"
                     /locus_tag="VFS75_03750"
     CDS             2213..4240
                     /gene="uvrB"
                     /locus_tag="VFS75_03750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU4677802.1"
                     /translation="MEFRLTTDKRPAGDQPKAIEALTAGVEAGMRHQTLLGVTGSGKT
                     FTVANVIAHVQKPTLVIAHNKTLAAQLAQEYKDFFPENAVHYFVSYYDYYQPEAYMPV
                     SDTYIEKEAMINEEIDRLRHASTQALLTRRDVIIVASVSCIYGLGSPVEYEKVHKKIE
                     RGFKTTRADLLRLLVSMQFERTNADLTPGHFRAVGNTVEIMPTNERVIYRVGFGGDEV
                     SSITKIDAISRAVIEESTVFFLFPAKHFVTPESERARAIEDIKAELKDQLKVFEREGK
                     LLEAQRLKRRTEHDLALIREIGYCNGIENYSRHFDGRLPGEPPHTLLSYFPHHEDGTP
                     DFLTVIDESHVTIPQLGGMFAGDRSRKETLVEHGFRLPSAIDNRPLNFDEFEERVGQR
                     IYTSATPGSYEFAHAKEPEGKIVEQIIRPTGLVDPVIDLRKVSEVGAYPGQVKDFIEE
                     AVKVIAGGTRVLVTTLTKQMAEDLSEFLTERGVSSKYLHSDVKTIERIGILSDFRRGT
                     FDCLVGVNLLREGLDLPEVELVAILDADKEGFLRSETSLIQTIGRAARNVNGRVILYA
                     DGVTGALKSAIEETERRRELQLAYNEKHGITPQTIMKEIRDITAYMNTEHDKAVSTLL
                     TVDAELYRKDPKGTLEEKRRQMEEAVKILDFETAAILRDEIRALEELRKKR"
     gene            4296..7067
                     /locus_tag="VFS75_03755"
     CDS             4296..7067
                     /locus_tag="VFS75_03755"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU4677803.1"
                     /translation="MERKARKVRSPASGRRARTSASASSSRKRRPAEDSTSGVSPVKR
                     LRARLVGSSGRKISIRDNTRKVEAKEADSTQRVGESEPVAVTKEPTRKEKRTHVRKHA
                     LIAHSHVQLALLSPFRFPMLREERFLSTIARHAGVFLVIVGAGLALFNLRYATGYALH
                     ETPPSRAQTIDSSFTVTGTGSLDSTAQTNYTAPDPTPGVRLSVEGGGSTLTNMAVIMV
                     TVPYASGVTLLSESAEGHIVTLGAAFMVDATTWRYYWDTKQSPDGPYHLRTVVKNQYG
                     TYEHLDDATYTVKNAIDEKLSAPETDPVTATTNSSGSADAPTSATGETTIATTTVSDT
                     TTVAVPAPVHDITITLGTSPLSAIAPLTVYTKDAKDVRLYARNTASMNLYSLGEAFDH
                     GSGAWRLDWNTLLVPDGDYKIQAKASYQDGTFAESLYMSRSVKNGEVSSLATTTVTSP
                     IEPTVTLRIDAKSPLSGLATIGVEVPGASWVELYAVPKSSLTPLFLGLASRSGDAWTY
                     AWRTQETPNGDYRVYARAKTAYGFVESGKMTVSVQNAVITSYTESQDTLISTLSKAQD
                     SLIKETDGSTTAIAPGAPKPVYIEPVTSFVRTIEASSTVKEAIGDRLAAYRLALNGKL
                     DDLALAIRMGDTARATTIKTEIDALRSSVIVDLSPQVGKKELVADIDKYLSQISFELE
                     ELTAKNESILKERIGDAAVKDSDKDGITDYDEVNLYKTNPFAADTDGDSFIDSAEIRN
                     GFDPNDSESQSLIAYESPKDSGTKRPDLLAVDSITTLTATTRSGSGLPKKALISGRGL
                     PNSYVTLFIYSTPVVVTVKTDAEGAWSYVFDKELDDGTHEVYVGITDNAGRVVAKSDP
                     LPFVKTAEAYTGSVTVAPAPAPADTSFVDERTLLLVASMAVVGLGLVLMLLGYHVRGR
                     REDSVTAAT"
     gene            7064..8398
                     /locus_tag="VFS75_03760"
     CDS             7064..8398
                     /locus_tag="VFS75_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4677804.1"
                     /translation="MIEYYIVAFQNGLAYVRKHPELALTLVLILVVPLAFLLSGQRFL
                     AASRDNVERLEKDRLGLLEDVFESYLHAVRFDGTAVHAELRRLGELNPDITAMELVRD
                     DADGLQLIASLDGEAGTAPEADPSAYHIADARPDESVIVATTENGVRYWETVKAVRGE
                     GNERYYVRVETSLAGIDALFASRVRTAYYWLFALLAVVLLLILRHVRLIDYAYLYSRT
                     KREAELKDMFTNMIAHELRAPLTAIRGYASLMLERHLDDATRGDATKVKDSSERLLSI
                     INDLLDVARLQSGRMSVNPTRTNVAGVIRSALDTMRAVATEKGVALGEDGVLSDAYAD
                     IDEKRLHQSLVNLISNAIKYTKSGTIAVSLDERRDRVEIRVKDTGMGISADDQKRLFA
                     PFFRVESEEVGAVVGTGLGMWITKQLIEVMKGSIAVESIRGVGTHIVLTFPK"
BASE COUNT         1760 a         2737 c         2611 g         1479 t
ORIGIN      
        1 cacacgctcg cgcaggagcg tctcgcgaag gaactcgtcg acgacggcgc cgacctcgtc
       61 atcggtcacc acccgcacgt cgtcgaggac gtcgcgctct accgggggaa gccggtattc
      121 tactcgctcg gcaacttcgt cttcgatcag tacttcagca cggacgtcat ggagggactg
      181 ggcgtcacgc ttacgctcga gaacgagtac gcagactatg cgctcacggg gtacgcgaca
      241 atggagagcc ggagcgcgcc gtacgtgatg agcacgacct cttcaagcgc actctacgcg
      301 cgcgtgcttc aaaacgtgcc cggagcggag ctcgaacgcg ggacgttctc ggtgccgcgg
      361 tagcgctgtt gcatgtttct cgaagaggga tatgatgggc gggtaatacc aacaatatga
      421 atttcttcaa taaaaccgag gtcgtgggac tcttcgcgag cgtggccgtc atggcgctcg
      481 ccctcgcctt cgttcgcttc aagaccgata tcttcgcaga ggcgccgctt cttcgcggcg
      541 actcgcagag cgccatcgtc gccgtgagct cggcgaccgg cgatgcgacg acgagcgcgc
      601 ttcgcggcgc cctcgaagag gcatcgacgg ggaagggcga cctcgtgaag ctcgtcgtcg
      661 atgacatcgt gatcggcacg ggtccgaaag tgaagaacgg tgatacggtc accgtgaact
      721 acgagggccg gacgcaaagc ggcgtcacct tcgacagttc gtatgagcgc gggacgccct
      781 tcacgttcac cgtcggtgcg ggtaaggtca tcgaggggtg ggagaagggt ctcgtgggca
      841 tgcaggtcgg cggtaagcgc attctcgtca tcccggcgga catggcgtac ggcaaccggc
      901 aggtgggcgt cattccggcg aactcgccct tggtcttctc gatcgagctt ctcaagatcg
      961 agtaacccat gacctcgccc atctcattcg agatactcaa acggaaaccc ggaacactcg
     1021 ctcgcgcggg tgttcttcgt acgcctcatg gcgacatcga gacgccggcc ttcatcgccg
     1081 tcggtacgaa gggaacggtg aagtcgctga agcccgatga cctcgaacgc tacgtcggga
     1141 acgagatgac gctcgcgaac acgtaccacc tctaccttca gccgggcgcg gacgtcgtga
     1201 aggaggcggg tgggctcaac cgcttcaccg gttggaagac accgaccatc accgactcgg
     1261 gaggttttca gatattctcg ctcggcgccg cgttcggaaa gggcgtcacg aagttcgcga
     1321 ccgcggagcg cggcgatgag agcgcgggca tacgtccgta ctcgaaggag ctcgcgacga
     1381 gccacgggaa gctctgcgtg atcgacgacg agggagtcac gttcacgtcc cacctcgacg
     1441 gctcgatgca ccgcttcacg gcggagcgtt cgatcgagat ccagcacgac atcggcgccg
     1501 acatcatcat cgcgttcgac gagtgcacct cgccgaacgc gccccacgac taccagaagg
     1561 aggccatgga tcgaacgcac cggtgggcga agcgctccat cctcgcgcac aagcgggact
     1621 acgatgcgct caaacggcag ggtatcttcg gcgtcgtcca gggtgggcgc tacgaagacc
     1681 tgcggcgtga gagcgcggct accctcgccg cgatggactt cgacgggttc ggcatcggcg
     1741 gctccttctc gaaggcggac ctcggtgaat cgctccgcgt ggtgaacgaa gttctccccg
     1801 aggacaagcc gaggcacctt ctcggcatcg gcgagccgga ggacctccgc gcaggcgttc
     1861 tcatggggtg cgacctcttc gactgcgtcc agccgacgcg tctcggccga acgggaacca
     1921 tctacgtcca tggaagggac ggcctcgata agctcaacct gacgaagagc gagtaccagc
     1981 gcgacttcgg gaagcccgac ccggcatgcg agtgccacgt gtgctcgaac tacacgcgcg
     2041 cgtacctcgc gcacctcttt cgcgcgaacg aaatgctcgg cccgcatctc gcatcgctcc
     2101 acaaccttca cgcgatcgtc gcgtacacga agtcgctccg cgccgagata ctcgcgagct
     2161 aagccgcggt ggtgcggctt tttttatatc gatttcgcgc tatactctcg ccatggagtt
     2221 tcgactcacg accgacaagc gaccggcggg cgaccagccg aaggctatcg aagcgctcac
     2281 tgcgggcgta gaggccggca tgcgccacca gaccctcctc ggtgtgaccg ggtcggggaa
     2341 gacgttcacc gtcgcgaacg tcatcgcgca cgtccaaaaa ccgactctcg tcatcgcgca
     2401 caataagacg ctcgccgcgc agctcgcgca ggagtacaag gacttctttc ccgagaacgc
     2461 cgtccactac ttcgtctcgt actacgacta ctaccagccg gaagcctaca tgccggtctc
     2521 ggacacgtac atcgagaagg aggcgatgat caatgaggag atcgaccggc ttcgccatgc
     2581 gtcgacacag gccctcctca cgcgccgcga cgtcatcatc gtcgcgtcgg tctcgtgtat
     2641 ctacggtctc ggttcgccgg tcgagtacga gaaggtccac aagaagatcg aacgcggatt
     2701 caagacgacg cgcgcggacc tcctccgcct cctcgtgtcg atgcagttcg aacgaacgaa
     2761 cgcggacctc acgcccgggc attttcgtgc ggtcggcaac accgtcgaga tcatgccgac
     2821 gaacgaacgc gtcatctatc gcgtgggctt cggcggggac gaggtctctt cgatcacgaa
     2881 gatagacgct atatcccgag cggtcatcga ggaatcgacg gtcttcttcc tctttcccgc
     2941 gaagcacttc gtgacgccgg agagcgaacg cgcccgcgcg atcgaggaca taaaggcgga
     3001 gctcaaggac cagctcaagg tattcgagcg cgagggtaag ctcctcgagg ctcagcgtct
     3061 taagcgccgt accgaacacg atctcgcgct cattcgggag atcggctact gcaacggtat
     3121 cgagaactac tcgcggcact tcgacggccg cctgcctggc gagccgcccc atacacttct
     3181 ctcgtacttc ccgcaccatg aggacggcac accggacttc ctcacggtca tcgatgagtc
     3241 gcacgtgacc atcccgcaac tcggcggcat gttcgcgggt gaccgttcgc gaaaggaaac
     3301 gctggtagaa cacggtttcc gactcccgag cgcgatcgac aaccgcccgc tcaacttcga
     3361 tgagttcgag gagcgcgtcg gccagcgtat ctacacctcc gcgacgccgg gttcgtacga
     3421 gttcgcccac gcgaaggaac cggagggcaa gatcgtcgaa cagatcatcc gcccgactgg
     3481 actcgtcgac cccgtcatcg accttcggaa ggtgagcgag gtcggcgcgt acccaggcca
     3541 ggtgaaggac ttcatcgagg aggcggtcaa ggtcatcgcg ggtggcacac gcgttctcgt
     3601 gacgacgctc acgaagcaga tggcggaaga cctctccgaa ttcctcaccg aacgcggcgt
     3661 gagctcgaag tacctccaca gcgacgtgaa gacgatcgag cgcataggca tcctctcgga
     3721 cttcagacgc ggtacgttcg actgcctcgt cggcgtgaac ctgctccgcg agggtctcga
     3781 tctccccgag gtcgaactcg ttgccatcct cgacgccgat aaagagggat tcctgcgctc
     3841 ggagacgtcg ctcatacaga cgataggccg cgccgcgcgt aatgtgaacg gacgagttat
     3901 cctctacgcc gacggcgtga ccggcgccct gaagagcgca atcgaggaga cggaacgccg
     3961 ccgcgagctc caactcgcgt acaacgaaaa gcacggcatc acgccgcaga ccatcatgaa
     4021 ggagatacgt gacatcacgg cgtacatgaa cacggagcat gacaaggcgg tatcgacgct
     4081 ccttaccgtc gacgccgagc tctatcgcaa agacccgaag ggcacgctcg aggaaaagcg
     4141 ccggcagatg gaggaggccg tcaagatcct cgatttcgaa accgcggcga tcctccgcga
     4201 cgagatacgt gcgctcgagg agctccggaa aaagcgatag ttatcgacac gaacgcggcg
     4261 tattttcggc agcacgctta tataatggtg gtgacatgga acggaaggcg agaaaggtgc
     4321 ggagccctgc ctccggccgc cgggcgcgta cgagcgcatc ggcttcttcg tcgaggaaac
     4381 gacgaccggc ggaggacagc acgtccggcg tatcgccggt gaagcgtctt cgtgcacgtc
     4441 tcgtcggctc atcgggaaga aagatatcca tacgcgacaa cacgcgtaag gtggaggcga
     4501 aggaggcgga ctcgactcag agagtcggtg agagcgaacc ggtcgccgtg acgaaagagc
     4561 cgacgcggaa ggagaagcga acgcatgtgc gaaagcacgc gctcatcgcg cactctcacg
     4621 tccagctcgc gcttctctcg ccgtttcggt tccccatgct ccgcgaggaa cgatttttga
     4681 gcacgatagc ccggcacgcg ggcgtattcc tcgtcatcgt gggtgccggt ctcgctcttt
     4741 tcaatctccg gtacgcgacc ggttacgcgc ttcatgagac gccaccgagt cgagcacaga
     4801 ccatcgactc gtcgtttacg gtgacgggta cgggctcgct cgactcgacc gcacagacga
     4861 actacacggc acccgacccg acgccgggcg tgcgactctc cgtcgagggt ggcggttcga
     4921 cgctcacgaa catggccgtc atcatggtca ccgtaccgta cgcctcgggc gtaacgctcc
     4981 ttagcgaatc ggcggagggc cacatcgtaa cgctgggtgc cgccttcatg gtcgacgcga
     5041 cgacatggcg ctactactgg gatacgaagc aatcacccga cggtccatat cacctgcgca
     5101 ccgtcgtgaa gaaccagtac ggaacgtacg agcatctcga tgacgcgacg tatacggtga
     5161 agaacgcgat cgatgagaag ctgtcggcgc cggagacgga tcccgtgacg gcgacgacga
     5221 attcgagcgg ctctgcggac gcgccgactt cggcgacggg ggagacgacg atcgcgacga
     5281 cgaccgtctc ggatacgacg acggtggccg tacccgcgcc ggtccacgac atcaccatca
     5341 ccctcggtac ctcgccgctt tccgccatcg ccccgctgac cgtatacacg aaggacgcga
     5401 aggacgttcg tctctatgcg cgcaataccg catcgatgaa cctctactcg ctcggcgagg
     5461 cgttcgacca cgggagcggc gcgtggcgtc tcgattggaa cacgctcctc gttcccgacg
     5521 gtgactacaa gatacaagcg aaggcctcgt accaagacgg cacgttcgcc gagagtctct
     5581 acatgtcgcg ctcggtgaag aacggtgagg tgtccagcct cgcgacgacg accgtaacct
     5641 caccgatcga gccgacggtc acgctccgta tcgacgcgaa gagtccgctc tcgggtctcg
     5701 cgaccatcgg cgtcgaagtt cccggcgcga gttgggtaga actctacgca gtgccgaaga
     5761 gctctcttac gccgctcttc ctcggtctcg cctcccgtag cggtgacgca tggacgtacg
     5821 catggcgaac acaggagacg ccgaacggcg actaccgagt gtacgcacgc gcgaagaccg
     5881 cgtacgggtt cgtcgagagc ggcaagatga cggtaagcgt gcaaaacgcc gttatcacgt
     5941 cgtacaccga gtcacaggat acgctcatct cgacgctctc gaaggcgcag gactccctca
     6001 tcaaggagac cgatggttcg acgacggcga tcgcgcctgg tgctccgaaa ccggtctaca
     6061 tcgagccggt cacgtccttt gttcggacga tcgaggcctc gagcacggtg aaggaagcga
     6121 tcggcgaccg gctcgccgcg taccggttag cgctaaacgg caagctcgat gacctcgcgc
     6181 tcgcgatccg catgggagat accgctcggg ccacgacgat caagaccgag atcgacgcgc
     6241 tccgctcgtc ggtcatcgtg gatctctcgc cgcaggtcgg gaagaaggag ctcgtcgcgg
     6301 acatcgataa gtatttgagc cagatatcgt tcgagctcga ggagctcacc gcgaagaacg
     6361 agagcatcct caaggagcgc atcggcgacg ccgcggtcaa ggattccgat aaggacggca
     6421 tcaccgacta cgacgaggtc aatttgtata agacgaatcc cttcgcggct gacacggacg
     6481 gcgactcgtt catcgacagc gccgagatcc ggaatggctt cgacccgaac gacagcgaga
     6541 gtcagtctct catcgcgtac gaatcaccga aggactcggg gacgaaacgc ccagacctcc
     6601 tcgccgtcga ttcaatcacc actctcacgg cgaccacacg ttccggctcc ggcctcccga
     6661 agaaggcgct catctccggc cgcggccttc cgaacagcta cgtgaccctc ttcatctaca
     6721 gtaccccggt cgtcgttacg gtgaagacgg acgcggaagg cgcgtggtcg tacgtcttcg
     6781 ataaggagct tgatgacggg acgcacgaag tgtacgtcgg tatcaccgat aacgccggcc
     6841 gcgtcgtcgc gaagagcgac ccgcttccgt tcgtgaagac cgccgaggcg tacacgggaa
     6901 gcgtgacggt ggcaccggca cccgctcccg ccgacacgtc cttcgtcgat gagcggacgc
     6961 tcctcctcgt cgcctcgatg gcggtcgtcg ggctcggact cgtgctcatg ctcctcgggt
     7021 accacgtccg cggacggcga gaggattccg tcaccgccgc gacatgatcg agtactacat
     7081 cgtcgctttc cagaacggac tcgcgtacgt ccgtaagcac cccgagctcg cgctcacgct
     7141 cgtcctcatc ctcgtcgtgc cgcttgcttt cctcctctcg ggtcagcggt tcctcgcggc
     7201 gtcgcgcgac aacgtcgaac gcctcgagaa ggaccggctc ggcctcctcg aggacgtctt
     7261 cgaatcgtac ttgcacgcgg tgcgattcga cggtaccgcg gtacatgccg agctacgtcg
     7321 actcggtgaa ctcaatcccg atatcaccgc gatggagctc gttcgagacg acgccgatgg
     7381 acttcagctc atcgcgtccc tcgacggcga agcgggtacc gcccccgagg ctgacccgtc
     7441 ggcgtaccat atcgcggacg cgcgaccgga cgagtcggtc atcgtggcga cgacggagaa
     7501 cggcgttcgc tactgggaga cggtgaaggc cgttcgtggc gaggggaacg agcggtacta
     7561 tgtccgcgtc gagacctcgc tcgccggtat agacgcgctc ttcgcgtctc gtgttcgcac
     7621 cgcgtactac tggctcttcg cgctcctcgc cgtcgtgctc ctcctcattc ttcggcatgt
     7681 acggctgatc gactacgcgt acctctatag ccgcacgaag cgcgaggcgg agctcaaaga
     7741 catgttcacg aacatgatcg cgcacgagct tcgtgcgccg cttaccgcca ttcgggggta
     7801 cgcgagcctc atgctcgaac ggcacctcga cgatgccacg cgaggcgatg cgacgaaggt
     7861 gaaggattcg tccgaacgcc ttctttccat aatcaacgac cttctcgacg tcgcacgttt
     7921 acaatctggc cgcatgtcgg tcaatccgac ccggacgaac gtcgcgggag tcatcaggtc
     7981 ggcgctcgat acgatgcgag cggtggctac cgagaagggc gtcgcgctcg gtgaggacgg
     8041 cgtcctctcg gacgcatacg ccgacatcga tgagaaacgt cttcaccagt ccctcgtgaa
     8101 cctgatatcg aacgcgataa aatacacgaa atccggcacg atcgcggtat ctctcgacga
     8161 gcggcgcgat cgggtggaga tccgggtgaa ggacaccggc atgggtatca gcgcagacga
     8221 ccagaagcgg ctcttcgcgc ccttcttccg cgtcgaaagc gaggaggtcg gcgcggtcgt
     8281 cggtaccggc ctcggcatgt ggatcacgaa gcagctcatc gaggtcatga agggttccat
     8341 cgcggtcgaa tccatacgcg gcgtcggcac gcacatcgtg ctcacgttcc cgaagtgatt
     8401 tttacagcga ccttcttttc cggtatactg tacgccttcc atacgcacac caccccacgc
     8461 accaccatgg ggcctattga tatggcaccg aagaaaccga gaaaacagca tacggatgag
     8521 aatcaccgcg acgagcgtat cgtgattcgt ggcgcgagaa cgcacaacct caagaacatc
     8581 gatctcg
//
LOCUS       DASTSP010000082        11749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1094492, whole genome shotgun sequence.
ACCESSION   DASTSP010000082 DASTSP010000000
VERSION     DASTSP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094492"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFS75_03765"
     CDS             complement(<1..412)
                     /locus_tag="VFS75_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEU4677805.1"
                     /translation="MIDFIGLGAQKSGTSWAYTCLYEHPAVCMPQKELHFFSRPRFEE
                     GKEWYEAQFKNCAPGKLRGEWSTSYLYSPDAPERIRSLYPNAKLLAILRNPIDRAYSQ
                     YRNAVRAGEIPKSMPFRAYSAKEPSVWEQGRYAEE"
     gene            complement(405..1445)
                     /locus_tag="VFS75_03770"
     CDS             complement(405..1445)
                     /locus_tag="VFS75_03770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4677806.1"
                     /translation="MRLLVYTQAVDKNDPILGFFHAWLLEFAKHFERIEVICLKEGAH
                     DLPSNVTVRSLGKEEGRGRISYVLRFYRYSTAAFLFGGVTHVFFHMGAVFNILAAPFF
                     LLRHTRNVTFYWWKTHGKANHLKERLALKCTDIVVTAGSQSFGLATNKVRVVGHAIDT
                     TLFSFSPLGERNPRACLAVGRIVPIKRMEVALETVEKLKDLPLSLTIIGNADNEVYGG
                     SLRTMTKDAGLAVEFLSARPQMEIVEYYKKANILLHPSFEAGFDKVVLEAMACGVIPL
                     TSIPSFRPILEPFGLFLPESEAASYASTVRRVVGLGHDDRMVLSERLRGIVRRDHSLE
                     TITGRIFGIRHD"
     gene            complement(1477..2913)
                     /locus_tag="VFS75_03775"
     CDS             complement(1477..2913)
                     /locus_tag="VFS75_03775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4677807.1"
                     /translation="MRNTDDSWLNPTKRAKDYLNKALEGDIEAEDLVVLNEEVKKKEE
                     ERKKAAEEKARQAALEPAPVVEETHEDEVAVDEARFSEPVLMEVTDREKIRLLIVTKD
                     VSILEENSISYQKIAGERTMFYELHIVILNVGGSKTDDPVIRLFENVWVYRTNSKLWW
                     TLIYDGYKVAESQLVFSGGFRADIVVGDDLFEAGLVAWYVAKKHGRPFQLHIGEDFFD
                     ETFVEEHEHPMLYRLAVWYLIRHAGSVRTHTEFQRQSVIEERPDLEAVTEVLPTYYDL
                     GVWRDLEPTVDLHVKYPQFKFIILHVSSMRPTSHALEALVGAAALLRRYPTLGLVMVG
                     NGPLRADLERQAIALGVQNQVEFEPMRPEVISYMKTANVLIQLSEDASEESLVLEGAV
                     SKVPLVANASGIAGKLFVDGESACLCAPSDIACVTNGLNRYLNENQERSSFAINASEI
                     VFERIEQDYGAYLEAYRESVERAILPES"
     gene            complement(2929..4047)
                     /locus_tag="VFS75_03780"
     CDS             complement(2929..4047)
                     /locus_tag="VFS75_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4677808.1"
                     /translation="MDVLMLSLGRKILERGSRERVRTEGYAKALSSLHIIVLTRREYA
                     VASETHEGTLHLYPTNSRSRFMMPIDAFRLALRVVETPRTEPLVISAQDPFILGWMCR
                     FLTLFPKTRFHIQVHGDYFAKGWAGRSLFRHFIRLFALILLKSAPAVRVVSERIRASL
                     VRRGVHADRITVLPIRPELESFLAVERVTRSEPPVRFLYVGRLAPEKDLPRILCAFAE
                     TLSRGCDATLRILGDGGERKRLEALIGSLRLTERVRILPWTADVPAAMAESDVLLLAS
                     KHEAYALVLVEAMAAGRPVVTTDVGCVGEVVEDNVHGIVVRDEGISPYAAAILRMCVD
                     ADFRRTCGERGRERARELARSDDETYVRGWVTALSRAL"
     gene            complement(4051..5154)
                     /locus_tag="VFS75_03785"
     CDS             complement(4051..5154)
                     /locus_tag="VFS75_03785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4677809.1"
                     /translation="MRLLIATGLYSPEIGGPATYTAFLERHLPSHGIGLTVVPFRIVR
                     GYPKLVRHIAYAFRLARAARRADVILALDTVSVGVPAMLASFLTRRPLYLRVPGDYAW
                     EQGQQRAGLHVTLDEYLENGPRPLLVRVLAWLQKRVASSAHRIIVPSEYLKGVVRHWG
                     IQGENVTVIHSAYHPIELKENRDELRARLKYEGYVVLTAGRLVPWKGMKTVIDAVALI
                     RASGILISLDIVGEGEEYEPLRRHIEELSASGFVHLRGREPKRILSERVKAADTFILN
                     TSYEGLSHQLLEVMDLGTPIVTTPVCGNIELIEDEKEGLLVPYDAKDAIVGALERLRT
                     DTGLRTKLTEAARARVRAFSEDEVVKDLVNFFS"
     gene            5217..6215
                     /locus_tag="VFS75_03790"
     CDS             5217..6215
                     /locus_tag="VFS75_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4677810.1"
                     /translation="MTTPKRPGTFVVSLDFELYWGMTDAATLDAYGANIRGVRTAIPR
                     MLDLFVRYGIHATWATIGMLMARSKTELLSLLPPPERRPTYVDPRLSSYEYLKTAPVG
                     EEESVDPYHFGSSLVERIKETPGQEIANHTFSHYYCIEGNAHLVETLGADLDAHARVS
                     ALHGVRTRSIVFPRNQVSPEALLVCAEHGITAYRGTENHFLYRPRRDEEQTLFVRALR
                     LFDHYVDVSGYHTYPTPVRTSDLPVNVPASRFFRPWIRALSLLEPLRMRRIKNAMTHA
                     AKRGEVFHLWWHPHNFGVNQDRNFKNLETILEHFASLRKTYGMESHSMAEVAETAA"
     gene            complement(6212..7012)
                     /locus_tag="VFS75_03795"
     CDS             complement(6212..7012)
                     /locus_tag="VFS75_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU4677811.1"
                     /translation="MEITGILHTHSTYSYDAKLSLTELRDLFVSRGLSFACMTEHVDE
                     MTEASADAFVKECRALSDSTFTFVPGFEVPYKRAHILMIGCERFFGNYAATVEALQPW
                     VGSTSFVVLAHPVRNRFVVEDALLSSLDAIEVWNQQYEGKRVPRTRSLSLLRHLREKD
                     PLLLSTGGVDFHRTEHLGGPFVTLEVGELAPEAILEKLKTGAYRVHSPQTSFYGTAPN
                     VDELIRAHRFESAFSVAIIVLGKTVNTVLAAFGISLPKSLKRLIRRKL"
     gene            complement(7026..8228)
                     /locus_tag="VFS75_03800"
     CDS             complement(7026..8228)
                     /locus_tag="VFS75_03800"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4677812.1"
                     /translation="MSAIVLDGHLKSALSAVRSLGKAGISLSVGAERGTAMALHSRYA
                     TSPFTYPSPLMDRAAFVDAVKREAMHRGGKPLIFAMSDATYLALYEARAEFEAVATLA
                     YPADESVALAFNKAATYSLAKVSGVPTIPTYESNTEGGVAEIARKLQYPAVVKPRRSV
                     TWRGAGGSFGTASFAMDEDDLKRLVRTATERFGEPPLVQEMVYGEEYGVEMLARSGVP
                     YARVTHHRIRSLSPKGGASVVKETLRSGELRSTLERYAAILVEKLAWNGPIMVEFKVD
                     HDSRTPYLMEVNGRFWGSLPLSVAARVDMPLLWYRAVMDGAIPEDVVTGREDVVTGHF
                     LGDVVHLVRVLFARDPMRPYLYPSRAQALRNFMLLPSGVRADVWSVLDPKPSLMEFVD
                     VIASFLKK"
     gene            complement(8225..9373)
                     /locus_tag="VFS75_03805"
     CDS             complement(8225..9373)
                     /locus_tag="VFS75_03805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4677813.1"
                     /translation="MRTRILVFSTDDHLFPAGGAEQAFGNIAERLPQIEFDLICARLR
                     PGVPKEETVGNVHIRRVGFGVPKLDGILLALFGHRVALRLMRKHQYSLMWSIMASYGA
                     FAAVRVKKRTNIPFLLTLQEGDSLEHIYERVRYVRGSFDEIFKLADGVQAISTFLLLW
                     AREMGFRGALGKVIPNGVDLKLFSKPYDGSDIKEMRESFNFPDGAFILVTSSRLERKN
                     GIGDVLMALSELPPHVCFVICGSGSLEQGLREYVREHDLSKRVRFMGFVEPAKLPLIL
                     RATDAFIRPSLSEGLGNAFLEAMSVRLPVIGTNAGGIPDFLKDRETGFVVEIGSPVSI
                     REKVLELMALHPSEKEAILKRAEALVRERYDWDLVAHSMEMLFKEVVP"
     gene            complement(9363..10241)
                     /locus_tag="VFS75_03810"
     CDS             complement(9363..10241)
                     /locus_tag="VFS75_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4677814.1"
                     /translation="MGGTGKKGLILVTGGAGFIGSHLAERLAKDGYRVVSLDDYSTGS
                     TENHVAGVEYREGHTKDIEALVPETPDVVFHLGEYPRVEQSFADIERVLSSNVIGTSA
                     VVEFCRKRNVKLVYAGSSTKFADGGIGRDQSPYAWTKATNTERVRNYGEWFGMPYAIA
                     YFYNVYGPRERGDAGSGTLIGIFKERFEAGKPLPVRTPGTQRRNFTHVDDIVEGLMLI
                     GERGAGDEYGLGHTDSYSVMEVAGLFGGPIEMLPERPGNRMTSQVDVNRARLELGWEP
                     KWDLPAYIASLKKHAH"
     gene            complement(10289..11545)
                     /locus_tag="VFS75_03815"
     CDS             complement(10289..11545)
                     /locus_tag="VFS75_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEU4677815.1"
                     /translation="MKLARLALTLLMIAPSVALAQVRINEVAWMGTAENANDEWIELF
                     NDGTDDVSLDGWTLSDGDTLSVTLSGVLFAGAYGVLERTDDDTVPGVSAFLIYTGALG
                     NDGRTLTLSRADGSTEDRVVGGDAWTNIGGDNETKATAQRTASGWTTAAPTPGERNEA
                     EEVAAAPEPEPEVTLSRSRGSGGGTTAPRAQKPVRPAPPPELSLGLDAPKTAYVGQPV
                     SFTANATGLPKPILDSLVYTWNFGDTDAGAGRTVTHTYAYPGEYVAVVDAAYGKHDEM
                     MRKDVTVLPVSLSLSRTASGDIELKNTASYEEDLGGFSLGGFTFPKYSFVKGKGTLIV
                     PTAKVGTRGPVSLRDTRGVAIASLSTSPQRSFITKANARISEPIAEANLEVVAPASTT
                     VIRIGNATTEEKLGFFARFYSMLFGS"
     gene            11544..>11749
                     /locus_tag="VFS75_03820"
     CDS             11544..>11749
                     /locus_tag="VFS75_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677816.1"
                     /translation="MVQSGGLSSVVRRIIRSSSFGVTPFCFGRGGSGVGAAFGAAVGS
                     SAGDACDEGFGSYFGAGFGSPLAA"
BASE COUNT         2258 a         3651 c         3623 g         2217 t
ORIGIN      
        1 gctcttcggc gtagcgcccc tgctcccata cgctcggttc ctttgcgctg tacgctcgaa
       61 acggcatgct cttcggtatc tcgcccgcac ggaccgcgtt tcggtactgc gaatatgcgc
      121 ggtcgatcgg gttccggagg atagcgagga gcttcgcgtt cgggtagagc gagcgaatgc
      181 gctccggcgc gtcaggcgag tagaggtacg atgtcgacca ttcccctctc agcttcccgg
      241 gagcgcagtt cttgaactgc gcctcgtacc actccttgcc ctcttcgaat cgcgggcgcg
      301 aaaagaagtg gagctccttc tgcggcatgc acaccgcggg gtgctcgtag agacacgtgt
      361 atgcccacga ggttcctgat ttctgcgcgc cgaggccgat gaagtcaatc atgtcgaata
      421 ccaaagattc gtcccgtaat ggtctctagc gaatggtcac gtcgtacgat acccctcaat
      481 cgctccgata gtaccatacg gtcgtcatgt cccagaccta ccacacgtcg aactgtcgag
      541 gcgtaggagg cggcttccga ttcgggaaga aacaagccga atggttccag tatcggtcgg
      601 aaggacggta tcgaggtcag cgggatcacg ccgcacgcca tcgcttcaag cacgacttta
      661 tcgaatccgg cttcgaatga tggatgcagg agtatatttg cttttttata gtattccaca
      721 atctccattt gtggccgcgc agagaggaat tcgacggcga gcccggcgtc tttagtcatc
      781 gtacgcagcg atcctccgta tacctcattg tccgcatttc ctatgatggt caatgatagt
      841 ggcagatcct ttaacttctc cacggtttct aacgccactt ccattctctt gatggggacg
      901 atcctcccta ctgcaaggca cgcgcgtggg tttcgctctc ctaatggaga gaacgagaac
      961 agtgtcgtat cgatagcatg accgacaaca cgcactttat tagtcgccag accaaaactc
     1021 tgacttcccg ccgtaacaac gatatccgta cactttagag cgagacgctc cttcaagtgg
     1081 ttcgccttac cgtgcgtttt ccaccaatag aacgttacat tcctcgtatg ccgcaggaga
     1141 aaaaagggag ccgccaaaat attgaagact gcgcccatat ggaagaagac atgggtcacg
     1201 cctccaaaca agaatgctgc cgtcgagtac cgatagaatc gaagcacgta cgagatgcgc
     1261 cctctgccct cctccttccc gagcgagcgg accgtgacgt tcgaaggtag gtcatgggca
     1321 ccctctttga ggcagatgac ctcgatacgc tcgaaatgct tcgcgaattc gaggagccac
     1381 gcatgaaaaa acccaagtat cgggtcattt ttatcgactg cctgcgtgta tacgagaagt
     1441 ctcatggcgg cgggtcaccc gcattgtaca gtctttttaa ctctccggaa ggatggctcg
     1501 ttccacgctc tcacggtacg cctcgaggta tgcgccatag tcctgctcga tccgctcgaa
     1561 gacgatctcg ctcgcgttga tggcgaaact cgaccgttcc tgattctcat tgaggtagcg
     1621 gttcaaaccg ttcgtcacgc aggcgatgtc cgagggggca cagaggcacg cggattcgcc
     1681 gtcgacgaag agcttccccg cgatgcccga cgcgttcgcg acgagcggca ccttcgacac
     1741 cgcaccctcg agaacgagac tttcctcgga agcgtcctcc gagagctgga tgaggacgtt
     1801 cgccgtcttc atgtacgaaa tgacctcggg acgcatgggc tcgaactcca cctggttctg
     1861 tacgccgagc gcgatcgcct gccgctcaag atcggcacgc aagggtccgt tccccaccat
     1921 gacgagcccg agggtcgggt accggcgaag gagcgccgcg gcgccgacga gcgcctcgag
     1981 agcgtgcgag gtcgggcgca tcgaggacac gtgaaggatg atgaacttga actgcgggta
     2041 cttgacgtgg aggtcgaccg tcggttcgag gtctcgccag acgccaagat cgtagtacgt
     2101 cgggagaacc tcggtcacgg cctcgagatc gggacgttcc tcgatgaccg actgacgctg
     2161 aaactcggtg tgcgttcgaa cgcttcccgc atggcggata aggtaccaaa ccgcaagccg
     2221 gtagagcatg gggtgctcgt gttcttcgac gaaggtctcg tcgaagaaat cctcaccgat
     2281 atggagctgg aagggccgcc cgtgcttctt cgcgacgtac cacgcgacga gtccagcctc
     2341 gaagaggtca tcacccacga cgatatccgc gcggaagcca ccgctgaaga cgagctggct
     2401 ctcggcgacc ttgtacccgt cgtagataag ggtccaccaa agtttcgagt tcgttcggta
     2461 cacccagacg ttctcgaaga ggcggatgac cgggtcgtcg gtcttcgagc cgccgacatt
     2521 gaggatgacg atatggagtt catagaacat cgtccgttct cccgcgatct tctgatacga
     2581 gatgctgttc tcttcgagta ttgaaacgtc tttcgtgacg atgaggaggc gtatcttctc
     2641 ccggtccgta acctccatga ggacgggctc ggagaaacgc gcctcgtcga ccgcgacctc
     2701 gtcctcgtgg gtctcctcga cgacaggcgc gggttcgagc gccgcctgcc tcgccttctc
     2761 ctcggctgcc ttctttcgct cctcttcctt cttcttgacc tcctcattga gcacgacgag
     2821 gtcctcggcc tctatgtcgc cttcgagcgc cttattgagg tagtctttcg cgcgcttcgt
     2881 gggattgagc cacgaatcat ccgtgttccg catggctcca ttataccgtt agagagcgcg
     2941 agagagcgcc gttacccacc cgcggacgta cgtctcatcg tcactccgag cgagctcgcg
     3001 agcgcgttct cttccccgct cgccacacgt tcgcctaaag tccgcatcga cgcacatgcg
     3061 taagatggcg gcggcgtatg gagaaatacc ttcgtctcga acgacgatgc cgtgaacgtt
     3121 gtcttcaacg acctcaccga cacaaccgac atccgtcgtg acgaccggtc gccccgcggc
     3181 catggcctcg acgagaacga gcgcgtacgc ctcgtgcttc gacgcgagaa ggaggacgtc
     3241 gctctccgcc atggccgcgg gcacgtcagc cgtccacggg agtatccgca ctcgctcggt
     3301 aaggcgaagc gagccgatga gcgcctcgag gcgcttccgc tcgccgccgt caccgagtat
     3361 gcgaagggtc gcatcgcagc cgcgcgagag ggtctcggcg aaggcgcaga ggatgcgcgg
     3421 taggtctttc tcgggcgcga gccgtcccac gtagaggaag cgaacgggag gttcgctccg
     3481 cgtcacccgc tcgaccgcga ggaacgattc gagctcggga cgaatgggaa gaacggtgat
     3541 gcggtcggcg tgcacgccgc gtcgaacgag cgacgctctg atgcgctccg agacgacgcg
     3601 gaccgcgggc gccgatttga gtagaatgag cgcgaagagg cggatgaaat gccggaagag
     3661 agagcgaccc gcccaaccct tcgcgaagta atcgccgtgc acttggatat gaaaccgcgt
     3721 tttcggaaag agcgtgagaa agcgacacat ccatccgaga atgaacgggt cctgtgccga
     3781 gatgacgagg ggttccgtgc gcggcgtctc cacgacgcga agcgcgaggc ggaaggcgtc
     3841 gatgggcatc atgaatcgcg aacgcgagtt cgtcgggtag agatggagcg ttccttcgtg
     3901 cgtctcgctc gcgacagcgt actcccgtct cgtgaggacg atgatgtgga ggctcgagag
     3961 cgccttcgcg tacccttcgg ttcgtacgcg ctcgcgcgac ccgcgctcga gtatcttccg
     4021 ccctaagctc agcatgagaa cgtccatggt ctatgaaaag aagttaacaa ggtccttcac
     4081 gacctcgtcc tcactgaagg cgcgtactct cgcgcgtgcc gcctccgtga gcttcgtacg
     4141 gagtcccgtg tcggtgcgaa gtcgctcgag cgccccgacg atcgcgtcct tcgcatcgta
     4201 tggaacgagg agaccttcct tctcgtcctc gatgagctca atattcccac agacgggcgt
     4261 cgtcacgatg ggcgtaccta ggtccatgac ctcgagaagc tggtgcgaga gaccttcata
     4321 ggacgtgttg aggatgaacg tgtcggccgc tttgacgcgt tccgatagaa tgcgcttcgg
     4381 ctcgcgaccg cgaaggtgga cgaagcccga tgcgctgagc tcctcgatat gtcggcggag
     4441 gggctcatac tcctcacctt cgccgacgat gtcgagcgag atgaggatgc ccgaggcgcg
     4501 gatcagagcg accgcgtcga tcaccgtttt catgcccttc cacggaacga gtcgccccgc
     4561 ggtgaggacg acgtacccct cgtacttaag gcgcgctcga agctcatcgc ggttctcttt
     4621 gagctcgatc ggatgatacg cactgtggat gacggtcacg ttctccccct ggatgcccca
     4681 gtggcgtacg acgcccttca aatactcgct cgggacgatg atgcgatgag ccgatgaggc
     4741 gacccgcttt tggagccacg cgagcacgcg aacgagaagt gggcgcggac cgttctcgag
     4801 gtactcgtcg agggtcacgt ggagtcctgc gcgttgctgc ccctgctccc atgcgtagtc
     4861 gcccggtacg cggaggtaga gcggccgcct cgtgaggaac gaggcgagca tcgcggggac
     4921 gccgacgctc accgtatcca acgcgagtat gacgtccgcc cgccgggccg cgcgcgcgag
     4981 tcggaacgcg taggcgatgt gccgcacgag cttcgggtag ccacggacga tacgaaacgg
     5041 cacgacggta agaccgatac catgcgaagg caggtgccgc tcgaggaatg ccgtatacgt
     5101 cgccgggccg ccgatctccg ggctatagag gccggtcgcg atgaggagtc tcatagccat
     5161 agtatgcatt agaaacgttg cttttaaaac acgctttcgc gctgtatagt taccgcatga
     5221 ccaccccgaa gcgacccgga accttcgtcg tctccctcga cttcgagctc tactggggca
     5281 tgaccgacgc ggcgacgctc gacgcgtacg gcgcgaacat ccgcggtgta cggaccgcca
     5341 tcccgcgcat gctcgatctc ttcgtccgct acggtatcca cgcgacgtgg gcgacgatcg
     5401 gcatgctcat ggcgaggagt aagacggaac tcctctcgct ccttcccccg cccgaacgtc
     5461 gcccgacgta cgtcgaccct cggctctcgt cgtacgaata tttgaaaacc gcgccggtcg
     5521 gcgaagaaga gtccgtcgac ccgtaccact tcggttcgtc cctcgtcgaa cgcatcaagg
     5581 agacaccggg acaagagatc gcgaaccaca cgttctcgca ctactactgc atcgagggca
     5641 acgcgcacct cgtggagacc ctcggcgccg acctcgacgc gcacgcgcgc gtaagcgcgc
     5701 ttcacggcgt ccggacgcgc tccatcgtct tcccacgaaa ccaggtgagc cccgaggcgc
     5761 tcttggtctg cgccgagcat ggcatcaccg cgtaccgcgg cacggagaac cacttcctct
     5821 accgtccgcg gcgggacgag gagcagacgc tcttcgtccg tgcgcttcgc ctcttcgatc
     5881 actacgtgga cgtaagcggc taccatacgt acccaactcc cgtgcggacc tccgacctcc
     5941 ccgtgaacgt acccgcgagc cgcttcttcc gcccttggat acgtgcgctt tccctccttg
     6001 agccgctccg catgcgacga ataaagaacg ccatgaccca tgccgcgaag cgcggcgagg
     6061 tcttccacct ttggtggcat ccgcacaatt tcggcgtgaa tcaagaccgt aacttcaaga
     6121 atctcgagac tatcctcgaa cacttcgcgt cgctacgaaa aacgtacggc atggagagcc
     6181 actcgatggc cgaggtcgcg gagacagcgg cctaaagttt tcttcgtatt agacgcttga
     6241 ggctcttcgg gagcgagatg ccgaaggcgg cgagtaccgt gttcacggtc ttcccaagga
     6301 cgatgatggc gaccgagaac gcgctctcaa agcgatgggc tcggatgagc tcgtcgacat
     6361 tgggcgccgt gccgtagaac gaggtctgcg gcgagtggac gcgatacgcg cccgtcttca
     6421 atttctcgag gatggcctcg ggggcgagct ccccgacttc gagggtaacg aaggggccgc
     6481 cgaggtgctc cgtgcggtgg aagtcgacgc ctcccgtaga gagaagaagt gggtccttct
     6541 cgcgaagatg tcgaaggaga gagagcgagc gcgtgcgcgg aacgcgcttc ccctcgtact
     6601 gctgattcca cacctcgatc gcgtcgaggg aagaaaggag cgcgtcttcg accacgaagc
     6661 ggtttctcac cggatgcgcg agaacgacga aggacgtgct ccctacccat ggctgaagtg
     6721 cctcgaccgt tgccgcgtag tttccgaaga atcgctcgca gccgatcatg agaatatgcg
     6781 cgcgcttata gggcacctcg aaaccgggga cgaacgtgaa cgtcgaatcc gagagcgcgc
     6841 ggcactcttt gacgaacgcg tccgctgacg cttcggtcat ctcgtcgacg tgctccgtca
     6901 tacacgcgaa cgagagccca cgcgagacga aaaggtcgcg aagttccgta agcgagagct
     6961 tcgcgtcgta cgagtaggtc gagtgcgtgt gaaggatgcc ggttatttcc atgaacggtc
     7021 cagtgttact tttttaaaaa cgaggcgatg acgtccacga actccatgag ggacggtttc
     7081 gggtcgagaa cggaccatac gtctgcgcga acgccggagg ggaggagcat gaaatttcga
     7141 agtgcctgcg cccggctcgg gtagagatac gggcgcatcg ggtcccgtgc gaagagcacg
     7201 cggacgaggt gcacgacgtc gccgaggaag tgcccggtta cgacatcttc gcgcccggtc
     7261 acgacatctt ccggtatcgc tccgtccatg acggcgcgat accaaagaag cggcatgtcg
     7321 acgcgcgcag cgaccgagag agggagcgag ccccagaagc gtccgttcac ctccatgagg
     7381 tatggtgttc gcgaatcgtg gtccactttg aattcgacca tgatgggacc gttccacgca
     7441 agcttttcga cgaggattgc cgcgtagcgc tcaagcgtcg atcgcagttc gccggaccgg
     7501 agggtctctt taacgacgga cgcgccgccc ttaggcgaga gcgaccgtat gcggtgatgc
     7561 gtgacccgtg cgtacgggac gccagagcgg gcgagcatct cgacaccgta ctcctcaccg
     7621 tagaccattt cctgaacgag gggcggctcg ccgaatcgct ccgtagcggt gcgaacgaga
     7681 cgctttaggt catcctcgtc catcgcgaag gacgcggtac cgaagctccc gcccgcgccg
     7741 cgccacgtga cgctccgccg tggcttcacg accgcggggt actgaagctt tcgcgcgatc
     7801 tccgcgacgc caccctcggt gttcgactcg tacgtgggga tggtagggac acccgagacc
     7861 ttcgcgagcg aatacgtcgc tgctttgtta aacgcgagcg cgaccgactc gtccgcgggg
     7921 tacgcgagcg tggcgacggc ttcgaactcg gcacgagcct catagagcgc gaggtacgtt
     7981 gcatcgctca tcgcgaagat gagcggcttt ccgccccggt gcatcgcctc tcgtttcacc
     8041 gcatcgacga acgcggcgcg gtccataagc ggcgacgggt acgtgaaggg cgacgtcgcg
     8101 tagcgcgagt ggagcgccat cgcggtcccc cgctcggcac cgaccgaaag tgagatcccc
     8161 gcctttccga gcgagcggac ggcagagagc gcgctcttca gatgtccgtc gagaacgatg
     8221 gcgctcatgg taccacttcc ttaaagagca tctccatgct atgtgccacg aggtcccaat
     8281 cgtaccgttc gcgaacgagc gcctccgctc ttttgagtat cgcctccttc tccgacgggt
     8341 gaagcgccat gagctcaaga accttctcgc ggatgctcac gggactcccg atctcgacga
     8401 cgaaacccgt ctcgcggtcc ttgaggaagt cggggatgcc gcccgcgttc gtgccgatga
     8461 ccgggaggcg caccgacatc gcctcgagga acgcgttccc gagaccttcg gagagagacg
     8521 ggcgaatgaa cgcgtccgtg gcgcgaagga tcaaggggag cttcgccggc tcgacgaacc
     8581 ccatgaaccg cacgcgcttc gagaggtcgt gctcgcggac gtactcgcga agcccctgct
     8641 cgagggaacc gctcccgcag atgacgaagc agacatgggg cggaagttcg gagagcgcca
     8701 tgaggacgtc gccgatgccg ttcttcctct cgagacgcga actcgtcacg aggatgaacg
     8761 cgccgtcggg aaaattaaag gactcgcgca tctcctttat atcgcttcca tcgtacggct
     8821 tcgagaagag cttgaggtcg acgccgttcg ggatcacctt ccccagggcg ccgcggaacc
     8881 ccatctcgcg cgcccagagg agaaggaacg tcgagatggc ctgaacgccg tccgcgagct
     8941 tgaatatctc gtcgaatgac ccgcgcacgt accggacgcg ctcgtagatg tgctcgagcg
     9001 aatcgccctc ctggagcgtg aggagaaacg ggatgttcgt ccgcttcttc acgcgaaccg
     9061 cggcgaatgc gccgtagctc gccataatgg accacatgag gctgtactgg tgtttccgca
     9121 tgagacgaag cgcgacgcga tggccgaaga gcgcgaggag gatgccgtcg agtttcggaa
     9181 cgccgaaacc gacgcggcgg atatggacgt tgcccaccgt ctcctctttc ggcacaccgg
     9241 gacgaaggcg ggcgcagatg aggtcgaact ctatttgtgg gagtcgctcg gcgatgttgc
     9301 cgaacgcctg ctccgcaccg cccgcgggaa agaggtggtc atcggtcgaa aagacgagga
     9361 tcctagtgcg catgtttttt gagagacgcg atgtacgcgg ggaggtccca tttcggttcc
     9421 cagccgagct cgagacgcgc gcggttgacg tcgacctgtg atgtcatacg gtttcccggc
     9481 cgctcgggga gcatttcgat gggaccgccg aagaggccgg cgacctccat gaccgagtac
     9541 gagtcggtgt ggccgagccc gtactcgtcg cccgcgccgc gctcgccgat gagcatgagc
     9601 ccctcgacga tgtcgtcgac gtgcgtgaag ttacgacgct gggtccccgg cgtgcggacc
     9661 ggaagcggct tccccgcctc gaatcgctcc ttgaagatgc cgatgagcgt ccccgagccc
     9721 gcgtcgccgc gttcgcgcgg gccgtacacg ttatagaagt acgcgatcgc gtacggcatg
     9781 ccgaaccact cgccgtagtt tcgaacgcgc tcggtgttcg tcgccttcgt ccacgcgtac
     9841 gggctttgat cgcggccgat gccgccgtcc gcgaacttcg tcgaggaccc cgcgtacacg
     9901 agcttcacgt tccgcttcct acaaaactcg acgaccgccg acgtgccgat gacattcgac
     9961 gagaggacgc gctcgatgtc ggcgaagctc tgctcgacgc ggggatactc gcccaagtgg
    10021 aagacgacgt caggcgtctc ggggacgagc gcctcgatgt ccttcgtgtg gccctcgcgg
    10081 tactcgacgc ccgcgacgtg attctccgtc gacccggtcg agtaatcatc gagcgagacg
    10141 acacggtagc cgtcttttgc gaggcgctcc gcgaggtgcg agccgatgaa gccggcgccg
    10201 ccggtcacga gaataagccc ttttttaccg gtgccgccca tacctatcgc ctcatagtac
    10261 caggaaaaac ggcggaatgt cacaccggct acgaaccaaa gagcatcgaa tagaaacgcg
    10321 cgaagaaccc gagcttctcc tcggtcgtcg cgttcccgat gcgaatgacc gtcgtcgacg
    10381 ccggcgcgac gacctcgagg ttcgcctccg ctataggctc ggagattcgc gcgttcgcct
    10441 tcgtgatgaa cgatcgctgt ggggacgtcg aaagcgatgc gatggcgacg ccacgcgtgt
    10501 cgcggagcga gaccgggccc cgcgtaccga ctttcgccgt gggaacgatg agcgttccct
    10561 tccccttcac aaaggagtac ttcggaaacg tgaagcctcc gagcgagaac ccaccgaggt
    10621 cttcttcgta cgaagcggtg ttcttgagct cgatgtcgcc cgacgcggta cgcgagagcg
    10681 aaagcgagac cgggagaacc gtcacgtcct tccgcatcat ctcatcgtgc ttcccgtacg
    10741 ccgcatcgac gacggcgacg tattcgccgg ggtacgcgta cgtatgggta accgtgcgcc
    10801 cagcacctgc gtccgtgtcg ccgaagttcc acgtatagac gagcgagtcg agaatgggct
    10861 tcggcaggcc ggtcgcgttc gccgtaaacg acacgggctg accgacgtac gccgtcttcg
    10921 gcgcgtcgag cccgagcgag agttcgggtg gcggtgctgg acgaacgggc ttttgagcgc
    10981 ggggcgcggt cgttcctccg ccactccctc gtgagcggga gagggtcacc tccggttcgg
    11041 gttcgggagc ggcggccacc tcctctgcct cgttccgctc gccgggcgtc ggcgcggcgg
    11101 tcgtccagcc ggacgcggtg cgctgtgccg tcgccttcgt ctcattgtcg ccgcctatgt
    11161 tcgtccatgc gtcgcccccg acgacgcggt cttccgtcga accatcggca cgagagagcg
    11221 tgagcgttcg gccgtcattc ccgagagcgc ccgtatagat gaggaacgcg ctgacgccgg
    11281 gaacggtatc gtcatcggtt cgttcgagaa cgccgtacgc gcccgcaaag agcacgcccg
    11341 aaagcgttac cgagagcgtg tcaccgtcag agagcgtcca gccgtcgagc gagacatcgt
    11401 ccgtgccgtc gttaaaaagc tcgatccatt catcgttcgc gttctcggcc gtccccatcc
    11461 atgcgacctc gtttatccgc acctgtgcca atgcgacgga gggcgcgatc atgaggagcg
    11521 tgagcgcgag gcgtgcgagt ttcatggttc aaagtggcgg tttgtcgtcg gtcgtgagac
    11581 gcatcatccg ctcgagttcc ttcggcgtca cgccgttttg cttcggacgc ggcggctctg
    11641 gcgtgggggc ggccttcgga gccgccgtcg gctcctctgc aggcgacgct tgcgatgagg
    11701 gcttcggttc ttacttcggc gcgggcttcg gctcccccct cgcggccga
//
LOCUS       DASTSP010000083         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1324371, whole genome shotgun sequence.
ACCESSION   DASTSP010000083 DASTSP010000000
VERSION     DASTSP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324371"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFS75_03825"
     CDS             complement(<1..528)
                     /locus_tag="VFS75_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4677817.1"
                     /translation="MRPFEIALIGIFGFIALVGLLFLANFKGGGSSSDLVYGSSVTIW
                     GTLDQKKMDDYLYKLSSENKALQVVTYKKIDARTFDTELVNAIAEGRSPDLVILPSSL
                     LVSYRTKLQALSAETMDPRVFRDTYIDGAEVFLMSDGAYGLPFAVDPLVMYWNRDLFS
                     SGGLATPPSTWESLVA"
     gene            complement(538..1086)
                     /locus_tag="VFS75_03830"
     CDS             complement(538..1086)
                     /locus_tag="VFS75_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4677818.1"
                     /translation="MYPRTFGKFAEPKTVASHFHLREGDSVADFGAGSGHYMEPLSAL
                     VGRTGSVYLCDIQKNLVDSLGARARERRLSNTHPMWADVETKGGVRLRDEALDAVLIS
                     NLLFQLHDREAGLREAARVLRKGGKLIVVDWTDSFGGLGPHPDHVVREADAKALGEKT
                     GFSFERTFPAGDHHYGIVFRKA"
     gene            complement(1188..>3026)
                     /locus_tag="VFS75_03835"
     CDS             complement(1188..>3026)
                     /locus_tag="VFS75_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU4677819.1"
                     /translation="DASTNDLLMFIKGPDFPLGGIAFNQKDIGHAYANGRGGVVVRGE
                     TEIVEDKKGNFSIVVTSIPYRVNKAELLMKIADLVRDKKIEGIRDLRDESTTDIRVVV
                     ELKSGAQPQTVLNKLYKHTQLEETFHYNMVALVDGVPQTLSLKAILEEYIKHRAEVVR
                     RRTQFDLTKAKEREHILLGLKKALDHIDAIIKLIRASKDVPTAHAGLMKQFKFSEVQA
                     QAILDMRLQKLAGLERKKIEDELKEMQALIAELESILASKKKMLGLVKSELTDIAERY
                     GDDRRTKIVKGGVKMLSVEDLVADEESVLVLTQGGYVKRTNPAEYRTQRRGGVGVVDL
                     NTKEEDVVTTLLTTSTHSDLLFFTNKGKVYQTKMFDIPEGRRATKGKSIMNFLALTAE
                     EKVTSVLAVRKEAWEGDWSLMLVTKDGVGKKSDASAFRDVRRSGLIAITLHDDDALIS
                     AKFVGKGDEMMLVTGKGQSIRFKESDVRQMGRTAAGVRVMKLGKGDRIVSADVLSKDT
                     KESEILVVMENGFGKMTPAKEYKVQNRSGSGIKTAKITSKTGDVIGAAIVSGEERTEG
                     ELVVMSKKGQVIKLPLKDVPSLGRQTQGVKVMKLRPGDSIASTVYF"
BASE COUNT          537 a          924 c          926 g          639 t
ORIGIN      
        1 ggctaccagg gactcccacg tcgacggcgg cgtcgcgagc ccgcccgagg agaagaggtc
       61 acgattccag tacatgacga gggggtcgac ggcgaacggg agaccgtacg cgccatcgct
      121 catgagaaat acctccgcgc cgtcgatgta tgtatcgcga aagacgcgcg ggtccatagt
      181 ctcagccgag agagcctgaa gcttcgtacg gtacgagacg aggagcgagc tcggtaggat
      241 gacgaggtcg ggtgagcgcc cctctgcgat agcgttcacg agctcggtat cgaacgtgcg
      301 cgcgtctatc tttttgtacg taacgacttg aagcgccttg ttctcgctcg agagtttgta
      361 gagatagtca tccatcttct tctggtcgag cgtgccccag atggtgacgc ttgagccgta
      421 gacgagatcg ctcgacgaac ccccaccctt aaagttcgcg aggaagagga ggccgacgag
      481 cgcgatgaag ccgaagatgc cgatgagcgc tatttcgaat ggtcgcatgt tcatggatta
      541 tgctttacga aagacgatac cgtagtgatg gtcccccgcg ggaaacgtcc gctcgaacga
      601 aaagccggtc ttctcgccga gcgccttcgc gtccgcctcg cggacgacgt ggtcgggatg
      661 tggaccgaga ccgccgaagg aatcggtcca atcgacgacg atgagcttcc cgcccttccg
      721 cagaacgcga gcggcctcgc ggagacccgc ttcgcggtcg tgaagctgaa agagcaggtt
      781 cgagatgagg accgcgtcga gagcctcgtc ccggaggcgc acgccgccct tcgtctcgac
      841 gtccgcccac atcgggtgtg tgttcgagag ccggcgctcg cgcgcgcggg cgccgagcga
      901 atcgacgagg ttcttctgaa tgtcgcagag atacacgctc cccgtccggc cgacgagcgc
      961 agagagcggc tccatgtagt ggccgctccc cgcaccgaaa tcggcgaccg agtcgccctc
     1021 gcgcagatga aagtgactcg cgaccgtctt cggctcggcg aactttccga acgtccgggg
     1081 gtacataccg tcattatagc gcgcggcgga cgtccgcgta cgcgaaggtg gtgaaaaacg
     1141 cccggaaatg cggagcattt ccgggcgttt atttcatatt cgaaccctta aaaatatacc
     1201 gtcgacgcga tcgaatctcc ggggcggagt ttcatgacct ttacgccctg cgtctgacgg
     1261 ccgagtgacg gcacgtcttt gagggggagc ttgatgacct gtcccttctt cgacatgacg
     1321 acgagctcac cctcggtgcg ctcctcgccg cttacgatcg ccgcgccgat gacgtcgccc
     1381 gtcttcgagg ttatcttcgc ggtcttgatg cccgaaccgc ttcggttctg caccttatat
     1441 tccttcgcgg gcgtcatctt gccgaatccg ttctccatga cgacgagtat ctcgctctcc
     1501 ttcgtatcct tcgagagaac gtccgcggag acgatgcggt cgcccttccc gagtttcatg
     1561 acgcgtacgc cggcagcggt gcggcccatc tgccggacat cgctctcctt gaagcgaatg
     1621 gactggccct tgcccgtcac gagcatcatc tcgtctccct tcccgacgaa tttcgcggag
     1681 atgagcgcat cgtcgtcgtg aagcgtgatc gcgatgaggc cgcttcggcg gacgtcccta
     1741 aacgcggatg cgtccgactt cttgccgacg ccgtccttcg tgacgagcat gagagaccag
     1801 tcgccttccc acgcctcttt cctcacggcg aggaccgacg tcaccttctc ctcggcggtg
     1861 agggcgagga agttcatgat ggacttaccc ttcgtcgcgc ggcgaccttc cgggatgtcg
     1921 aacatcttcg tctggtagac cttgccctta ttggtgaaga agaggaggtc gctgtgggtc
     1981 gacgtcgtga ggagcgtcgt cacgacgtcc tcctccttcg tgttgaggtc cacgacgccg
     2041 acgcctccgc ggcgctgtgt gcggtactcc gcgggattcg tgcgcttcac gtatcccccc
     2101 tgcgtgagga cgaggacgct ctcctcgtct gcgacgaggt cctcgacgga gagcatcttc
     2161 acgccgccct tcacgatctt cgtgcgacgg tcgtcgccat agcgctccgc gatgtcggtg
     2221 agttccgact tcacgaggcc gagcatcttc ttcttcgagg cgaggatgct ctcgagctcg
     2281 gcgatgagag cctgcatctc cttcaattca tcctcgatct tcttccgctc gaggccggcg
     2341 agcttctgga gccgcatatc gaggatcgcc tgcgcctgta cctcggagaa cttgaactgc
     2401 ttcatgaggc cagcgtgcgc ggtcggcacg tccttcgagg cgcggatgag cttgatgatg
     2461 gcgtcgatgt gatcgagcgc cttcttgaga ccgaggagaa tgtgctcacg ctccttcgcc
     2521 ttcgtgaggt cgaactgcgt ccttcgacgt acgacctcgg cgcggtgctt gatgtactcc
     2581 tcgaggatgg cctttaagga aagcgtctgc gggacgccgt cgacgagggc gaccatattg
     2641 tagtggaacg tctcctcgag ctgcgtgtgc ttgtagagct tattgaggac cgtctgcggc
     2701 tgtgcgcctg acttcagctc gacgacgacg cggatgtcgg tcgttgactc atcgcgaaga
     2761 tcgcggatgc cctctatctt cttgtcgcga acgagatcgg cgatcttcat gaggagttcc
     2821 gccttattga cgcgatacgg tatcgaggtg acgacgatgg agaagttccc cttcttatct
     2881 tcgacgatct cggtctcgcc gcgcacgacg acgccgccgc ggccgttcgc gtacgcgtga
     2941 ccgatgtcct tctgattgaa cgcgatgccg cccaagggga aatcgggccc cttgatgaac
     3001 atgagcaggt cgttcgtcga agcgtc
//
LOCUS       DASTSP010000084         5609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_653631, whole genome shotgun sequence.
ACCESSION   DASTSP010000084 DASTSP010000000
VERSION     DASTSP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653631"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..447
                     /locus_tag="VFS75_03840"
     CDS             133..447
                     /locus_tag="VFS75_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011790518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU4677820.1"
                     /translation="MKVSSGVVHTLPADLKKALVASPRALSLWEGLTPLARNEWICWT
                     ISVKQEKTRKDHVRRVVSELLEGMRRPCCWIGCIHRTDKAVSASVRGILEKRSKENRT
                     HR"
     gene            complement(490..2769)
                     /locus_tag="VFS75_03845"
     CDS             complement(490..2769)
                     /locus_tag="VFS75_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU4677821.1"
                     /translation="MEWQEEHLSDRPSRVLKWVLLGVGIGTFIILIWATLQTYKFAPP
                     RPNTFLSPSGEVLMTADDIVAGKGGFQRADLMDYGSIYGMGSYFGIDYTAEYLVELGR
                     RTEENLAEERYGVSFSSLREGERAEITAQMKRDLQSIDMTKDTVHVPTGLAEALASMR
                     GEVATSLRTDDFAKGYTAARSLDEELALQTADFLLYSSLTTVARRPGTESSWTQNWPY
                     EPSVGNTPTSSTFLWTWVSFCFTFFAFGLVIFIYERYLNVRDDAPREHILTDFRPLTP
                     SQRKTGKYFIVVAAVFLLQLAAGTLMAHYYAERTGFYGVDIASFLPFNFLRAIHVQAP
                     IVWIGLAWIGGALFIGPAISKREPRGQGVLVDILFWATVTVVVGALVGNYLGIMNVIG
                     DKGWFWFGNQGLSYIELGRAWQIGFFGGLLLWSILVMRALWPTWERFKKATWQFWTGA
                     IQLENLLWFATLNIALLYVFGMIPLTGIEDSFSITDFWRWWVVHLWVEQSFEFFAVAI
                     SAYLLMETGLVSRILAQRAVYFELILIFLGGVIGTGHHFYWVGGPGLWVPMGSMFSFI
                     EVLPLVLLITESISQYRLIRQHHKFEYKLAYTFIIGAAFWNFVGAGVFGGGTINAPLV
                     NYYEHGTFLTLNHAHTALFGAFGLLAIGFVYYCLRYAAANRFEFSHRLGLWAFWLYNA
                     GLVLWIFLNFFPIGWPQLVAVYEHGYAYARSLAFYDTTLLWQWLRVPGDVLFGAGALL
                     MAWDILRKLKPLYTKGNID"
     gene            complement(3016..3102)
                     /locus_tag="VFS75_03850"
     tRNA            complement(3016..3102)
                     /locus_tag="VFS75_03850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3066..3068),aa:Ser,seq:tga)
     gene            3312..3704
                     /locus_tag="VFS75_03855"
     CDS             3312..3704
                     /locus_tag="VFS75_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HEU4677822.1"
                     /translation="MRLMGIDYGTKRVGIALSDDKGLMAFPHVVLKNDSELVGAIETI
                     ATERSVGKIVIGHSLDREGKPNAVHADVEELIGDLTLALGVPIELEPEQYTTKEAIRF
                     QGRTDMTDASAAAVILNSYIMRTRNSKR"
     gene            3701..4048
                     /locus_tag="VFS75_03860"
     CDS             3701..4048
                     /locus_tag="VFS75_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677823.1"
                     /translation="MITYDDFIKVEIRIGEIRSVEVVEGADKLLRLMVDVGEGVGEDG
                     TPVLRQILSAIRTYFEDPQVLVGQKYPFVTNLEPRTIRGFESHGMILATSHEGTLALL
                     QPSADVPAGTKVK"
     gene            4105..5199
                     /gene="pilM"
                     /locus_tag="VFS75_03865"
     CDS             4105..5199
                     /gene="pilM"
                     /locus_tag="VFS75_03865"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEU4677824.1"
                     /translation="MSVLSFVERVFPPPDVMAFPCVGVDISDTSLKYIQFEHRSILDD
                     ELSLKYWGNVDIPDGIVERGSVHDVDKLTAVLKEVRNRCGARYVNVSLPEERAYLFET
                     TIASSTAPKDVRGLLEFKLEENVPLSPRDAYFDYAFVGEDEENKQTRVAVAVYARSTI
                     NSYFEACSKAGLMPLSFEIEAQAIARASVSKSITDTCLIVDFGKTRMGVGIVHRGMLM
                     YTSTIEIAGSQMSADMRAVLGDLPEAELTNIKNTRGLVRSAENEKVAKVLEKYAASIA
                     DELGIRIHYWNTRGIDPDTREIKKVIICGGSANLLGLPEYLTEKLDIPAERAQVWTNA
                     FSIEKTVPPITRRYSYGYATAIGLALRNFL"
     gene            5196..>5609
                     /locus_tag="VFS75_03870"
     CDS             5196..>5609
                     /locus_tag="VFS75_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677825.1"
                     /translation="MINLIPPEGQQAVKREYLLRVGGTLAFLFGGVLLALAVSLLPTY
                     VLVDAQISASASNARKAGADDEQFKSADLEVSRTSAILNQLQKTPPQPPMSAAIEDIR
                     AVAPAGIGFRNFFIEDDAGKVSKVQVQGVAATREVL"
BASE COUNT         1221 a         1639 c         1730 g         1019 t
ORIGIN      
        1 gtgcaggtac tcccgcaggg aggtatcgag gtgagcgatt acggaagtta cgatacgcag
       61 agcaaggaga aggacgtcat ggtgaccgtc cgtgcgtcgt tcgagcttta agagcatagc
      121 gcgccgaacc atatgaaagt ctcttcggga gtggtccata cgctaccggc ggaccttaag
      181 aaggcgctcg tcgcgtcgcc tcgagcactg tcgctctggg aaggtctcac gccgctcgca
      241 cgaaacgagt ggatctgctg gacgatctcc gtaaagcaag agaagacgcg gaaggaccac
      301 gtgcgtcgtg tcgtctccga actcctcgag gggatgcgtc ggccgtgctg ttggatcggc
      361 tgtatccacc gtacggacaa agcggtcagc gcttcggtga ggggcattct cgaaaagcgt
      421 tcgaaagaga ataggacaca tcgataaaag agattcgcat gagcgggtct ctttcgtcac
      481 gaccttcggt cagtcgatat tccctttcgt atacagaggc ttgagcttcc taagaatgtc
      541 ccaggccatg aggagcgcgc ccgcaccgaa gagaacgtct cccggtacgc ggagccattg
      601 ccagaggagc gtcgtatcgt agaacgcgag gctacgcgcg tacgcatacc cgtgttcata
      661 gacggcgacg agctgcggcc acccgatggg gaagaagttg aggaagatcc acaggacgag
      721 gcccgcgtta tagagccaga acgcccagag accgagccgg tgcgagaatt cgaatcggtt
      781 cgcggcggcg tagcggagac agtagtacac gaacccgatc gcgaggagcc cgaaggcgcc
      841 gaagagcgcg gtgtgcgcgt gattgagcgt gaggaacgtc ccatgctcgt agtagttcac
      901 gagcggcgcg tttatcgtgc cgccgccgaa gacaccggcc ccgacgaagt tccagaacgc
      961 ggcgccgatg atgaacgtat acgcgagttt gtattcgaac ttgtggtgtt ggcgaatgag
     1021 ccggtactga ctgatcgatt cggtgatgag gagaacgagc gggagaacct cgatgaacga
     1081 gaacatgctt cccatcggca cccagaggcc cggcccgccg acccagtaga agtggtggcc
     1141 ggttccgatc acgccaccga ggaagatgag gatgagctcg aagtatacgg cacgctgcgc
     1201 gaggatgcgc gatacgaggc cggtttccat gaggagatac gcgctgatcg cgaccgcgaa
     1261 gaactcgaac gattgttcga cccagaggtg gaccacccac cagcgccaga agtcggtgat
     1321 cgagaacgaa tcctcgatgc cggtgagcgg tatcatgccg aagacgtaga ggagcgcgat
     1381 gttgagcgtc gcgaaccaga ggaggttctc gagttggatg gcgccggtcc agaactgcca
     1441 cgtcgccttc ttgaagcgtt cccatgtcgg ccagagggca cgcatgacga gaatgctcca
     1501 gaggaggaga ccaccgaaga accctatctg ccacgcgcgc ccgagctcga tgtacgagag
     1561 cccttgattg ccgaaccaga accagccctt gtcgccgatc acgttcatga tgccgaggta
     1621 gttgccgacg agggcgccga cgacgacagt gaccgtcgcc cagaagagga tgtcgacgag
     1681 aacgccctga ccgcgcggtt cacgcttcga gatcgccgga ccgatgaaga gcgcgccgcc
     1741 tatccaagcg agacctatcc atacgatagg agcctggacg tggatggcgc ggaggaagtt
     1801 gaagggaagg aacgacgcga tgtcgactcc gtagaagccc gttcgctcgg cgtagtagtg
     1861 cgccatgagc gttcccgccg cgagctggag gaggaaaacg gcggcgacca cgatgaagta
     1921 cttgcccgtc ttacgctggc tcggcgtgag cggccggaag tccgtgagga tgtgctcgcg
     1981 cggtgcgtcg tcgcggacgt tgaggtatcg ctcgtagatg aagatgacga gaccgaatgc
     2041 gaagaacgtg aaacagaagc tcacccatgt ccagaggaag gtgctcgagg tcggcgtgtt
     2101 gccgaccgag ggctcgtacg gccagttctg cgtccacgag ctctcggtgc ccggccggcg
     2161 cgcaacggtc gtgagcgatg agtagaggag gaagtccgcg gtctgaagcg cgagctcctc
     2221 atcgagcgag cgtgccgccg tgtacccctt cgcgaagtcg tcggtgcgaa ggctcgtcgc
     2281 tacctcgccg cgcatcgaag cgagcgcctc ggcgagtccc gtcgggacgt gtaccgtatc
     2341 cttcgtcatg tcgatgctct ggagatcgcg tttcatttgt gcggtgatct ccgctcgttc
     2401 gccctctcta agcgacgaga acgacacgcc gtagcgttcc tccgcgagat tctcctcggt
     2461 tcgccgcccg agttcgacga ggtactccgc cgtgtaatcg atgccgaagt acgaacccat
     2521 gccgtatatg ctcccgtagt ccatgaggtc cgcacgctgg aaaccgccct tacccgcgac
     2581 gatgtcgtcg gcggtcatga ggacctcgcc cgagggggag aggaaggtat tcggtcgagg
     2641 tggtgcgaac ttgtaggtct ggagcgtcgc ccagatgagt atgatgaacg ttccgatgcc
     2701 gactccgagc aacacccact tgagaaccct gctcgggcgg tcgctcaaat gttcttcctg
     2761 ccattccata ctggctctaa gattatgcgt ctaataccgt aactgtagcg cgcagagacg
     2821 ggcgccatca atacccatcg atgtatatct atgcgaatca agaagagaga tcggtctcat
     2881 aaaacgggtg cggagggagc gagattcgaa acttacagtt tcagtacacc tttcgcagca
     2941 actgctcaag gtcttcgaat ctcttgcgca gaaactaaaa aatccaggga gggaccctga
     3001 atttttctag ttttgcggag ggagcgagat tcgaactcgc gagggacttt catccctaac
     3061 agttttcaag actgccgcct tcaaccactc agccatccct ccgggttatc tgcatctcgc
     3121 aggtggttgg cttcatccac ctgtcgcctc gacacgtcgg cgactccgcg aatcgcggaa
     3181 cggatgagaa atgaagcagt tcatttctcg tccgctcagc catccctcaa tatatgcacg
     3241 cttgtacata tatatgtacg gcggatacta gcagaaaacc gccgcgaatc aaagtatggt
     3301 attctgacgc catgcggctt atgggcatcg attacggcac gaaacgggtg ggaatcgccc
     3361 tctcggacga caagggcctc atggcgtttc cacacgtcgt cctcaaaaac gacagcgagc
     3421 tcgtcggcgc gatcgagacg atcgcgacgg agcgctcggt cggaaagatc gtcatcggtc
     3481 attcgcttga cagggagggg aagccgaacg cggtccatgc ggacgtcgag gagctgatcg
     3541 gcgacctcac gctcgccctc ggcgttccga tcgagctcga acccgagcag tacacgacca
     3601 aggaggccat ccgcttccag gggaggacgg atatgaccga cgcgagcgcc gcggccgtca
     3661 tcctcaacag ttacattatg agaacgagaa attccaaacg atgataacgt acgacgattt
     3721 cataaaagtg gagatacgga tcggcgagat acggtccgtc gaggtcgtcg aaggggccga
     3781 caagcttctt cggcttatgg tggacgtcgg ggagggcgtc ggcgaggatg gaacgcccgt
     3841 cctccggcag atactctccg ccatccggac gtacttcgag gacccgcagg tgctcgtcgg
     3901 gcagaagtat ccgttcgtga cgaacctcga gccgcgcacc atccgaggct tcgaaagtca
     3961 cgggatgata ctcgcgacga gtcacgaggg gacgctcgcg ctccttcagc cctcggcgga
     4021 tgtccccgcc ggtacgaagg tcaaatagta cgacctcatc aatttacgaa cggggaggcc
     4081 ccccgtataa tgaaaggacg atatatgtcg gttctttctt tcgtagagcg cgtatttcct
     4141 cctccggacg tcatggcgtt cccctgcgtc ggcgtcgaca tctccgacac gtctctcaag
     4201 tacatccagt tcgagcatcg gagcattctc gacgacgaac tctcactcaa gtattggggg
     4261 aacgtcgata tccccgacgg catcgtcgag cgcgggagcg tgcacgacgt cgataagcta
     4321 accgcggtcc tcaaagaggt gcggaatcgc tgtggcgcac ggtacgtgaa cgtctcgctc
     4381 ccggaggagc gcgcgtacct cttcgagacg accatcgcgt cgtcgaccgc gccgaaggac
     4441 gttcgcggcc ttctcgagtt caagctcgag gagaacgtac cgctctcgcc tcgggacgcg
     4501 tacttcgact acgcgttcgt cggggaagat gaggagaaca aacagacccg cgtcgccgtc
     4561 gccgtctatg cgcggtcgac catcaatagc tacttcgagg cgtgcagtaa ggcgggactc
     4621 atgccgctct ccttcgagat cgaggcgcag gcgatcgcgc gcgcatccgt ttcgaagtcc
     4681 atcaccgaca cgtgcctcat cgtcgacttt gggaagaccc gcatgggcgt cggcatcgtc
     4741 cacagaggta tgctcatgta cacctcgact atcgagatag cgggaagcca gatgagcgcc
     4801 gacatgcgag cggtcctcgg cgatctcccc gaggccgagc tcacgaacat caagaacacc
     4861 cgaggcctcg ttcgttccgc ggagaacgag aaggtggcga aggtgctcga gaagtacgcg
     4921 gcgtcgatcg cggacgaact cggcatacgc atccactact ggaacacgcg aggcatcgac
     4981 ccggacactc gtgagataaa gaaggtcatc atctgcggcg ggagcgcgaa ccttctcggc
     5041 cttcccgagt acctcaccga gaagctcgac atccccgcag aacgcgccca ggtatggacg
     5101 aacgcgtttt cgattgagaa gacggtgccg cccatcacac gccgatactc gtatgggtat
     5161 gcgacggcca tcggtctcgc gctacggaac ttcctatgat caatctcatc ccacccgaag
     5221 gacagcaggc ggtgaagcgc gagtacctcc tccgcgtcgg cggtacgctc gcgttcctct
     5281 tcggcggcgt gctcctcgcg ctcgcggtct cgctcctccc gacctacgtg ctcgtcgatg
     5341 cgcagatcag cgcgtcggcg tcgaacgccc gaaaggccgg cgcggacgac gagcagttca
     5401 agtcggccga cctcgaggtc tcccgaacga gcgccatcct aaaccagctc cagaagacac
     5461 cgccacaacc accgatgagc gcggcgatcg aggacattcg cgccgtcgca cccgccggca
     5521 tcggctttcg gaacttcttc atcgaggatg atgcgggaaa ggtctccaag gtgcaggtcc
     5581 agggcgtcgc cgcgacgcgt gaggtgctc
//
LOCUS       DASTSP010000085         6792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1108167, whole genome shotgun sequence.
ACCESSION   DASTSP010000085 DASTSP010000000
VERSION     DASTSP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108167"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1396
                     /locus_tag="VFS75_03875"
     CDS             188..1396
                     /locus_tag="VFS75_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4677826.1"
                     /translation="MLLPDLARLSTRAFKTNAMRTWLTILGMGVGTGAVVVLVGLGFG
                     LQQIILEQIVFGETLLSLGVSATGAHNLVIDDEAVAKFKAMDHVLNAAPLARFPALVT
                     YKGLTGNIFLQGAEPAYVRYAGITASAGDVFTDDDAANGSEVMLSPAILKLFGVEDPE
                     SFIGQEVTFKILVPANDGTPNVNQVNIDKKYKVKGITKEEGVLNVLMMLPELRNYVGV
                     DQYDRAQVRVDASENLDPVTTELVKQGYRVTALSKTVEQASKIFQGIQVTLAVFGGIA
                     LIVSAIGMFNTMTVTLLERTKEIGIMRTIGASPRDIKYLFVSESIIVGFLGGISGIVM
                     GVTLGFIVNIMLNVAASQFGGKAVQLFQFPLWFLAFIAIFSAAVGYLTGIFPARRASL
                     LNPLDAIRYE"
     gene            1467..3308
                     /locus_tag="VFS75_03880"
     CDS             1467..3308
                     /locus_tag="VFS75_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677827.1"
                     /translation="MSYNTDHLRTAGKVTVYTTLVGVIAFAIVFIFNLGEPSIKQADA
                     QSQATTTVTVLNTPPIWTASTTELVESSSNNPTNAGDTVSWVGIGTDSNGEDYYLLIC
                     DTNATPTPNSGGAPTCSSGVQWAVSTATLSGTQALAATTTYDALSEVNTWFAWICDSN
                     VISPRCNSDYTQGTNATNSSPFEVNHRPSFTAFVDDSPKDPGQTVTFTATASDADVSG
                     VADTVFLTVCATAGYSTTTDTCTGTTLATSTMFFSSNATSTYTIVIPTQDTNYSAFGY
                     IMDNHGFEASGGAQGTDSTLTVNNVAPTVSPALISLVQPSVTDMFLTVEAGETTGFQL
                     SFTTADNNSCDAVGGGSADEITGYNLSIYRSGVGSTTCTTAAGDYDANNCYPSGLATS
                     TWNLTCTASSTTCSGSSDTDMQWDCTFPLWYIADPTDGTATSTQYSTEDWVAQVQGID
                     DDAATGPLSEASSGVDVRSLLAFALNTLTIPYGSLEPGQQTDPLNATTTISATGNVGL
                     DKDVEGSSMCTTYTGSTPCPNSATSTIPENMQVVATSTVAYAQGTAISSTTPTFVDIN
                     VHKSIATSTQEQGSAYWGIKVPATITFAGDYTGQNTITALVSDSSQW"
     gene            3421..4344
                     /locus_tag="VFS75_03885"
     CDS             3421..4344
                     /locus_tag="VFS75_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677828.1"
                     /translation="MKGLRSFLAVMALFVVSAQYAYAGFGVTPPYVSNVSLTRNSVYE
                     QTIYLVRSDPSTDLKATVSVDVPGINDWFTINEGNEFLLPAGEQKVPMTVHIAVPDNA
                     DFKKYDGNIRIKTGPPDDKVAGGAVSISLGAQIDVNLVVIDKEIKDFKVRKIGISDLN
                     EGHKVGWLYFPGKIRFEMLIENIGNVPVAPSDVVFRIYDRSGNVLLEETHKKGRITNV
                     DPFKTESVFAELPTKLPAGSYLARFEIKNGDETKLSGEVNVSILPYGTLQAAGFGFLG
                     LSLAHQLSIVVPILFLLVAAMLIYVRTRRRR"
     gene            4351..5502
                     /locus_tag="VFS75_03890"
     CDS             4351..5502
                     /locus_tag="VFS75_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HEU4677829.1"
                     /translation="MIRRFAYTLPWTARLFLVVLSFVALALTHTALAQTVVGTSISTT
                     TLRISICGDSLVNAGEECDVPGDTGQYSTTIAGRQCTDQCLWAPYCGDGILQTLFSEE
                     CDDGNNDDGDFCSAICKVEPAGSGGGHVGGGGSSGGGGSDTRLGDTQVSIVGKAYPNV
                     SVTILVDGDVVGTVRTNSSADFSFNTDTKPGTASLSFWATDSKGTRSLTISTTFDVTQ
                     GAVTNVNGIFVPPTIRTSNANPNPGETITLSGQTIPNATVEVLVDSGAKRLTATANAS
                     GEWSVPFDTGTVSASTHTVKARFTTVNGALRTESQYGTALSIFVGVEGKPTSSADLNR
                     DGKVNLTDFSILVFWWGTPGGNSNPPADINGNGRVSLEDFSILLFNWTG"
     gene            5784..>6792
                     /locus_tag="VFS75_03895"
     CDS             5784..>6792
                     /locus_tag="VFS75_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677830.1"
                     /translation="MIHTAHTVRTLQAVAMTVGLSILLWSTGLPTIFRTAEAASITNA
                     SDTLSTSAPGNPANHTIEFTTPNGMAVGETITVTFPTGANEFVMGSVGEDDVDILVNG
                     SSSSTAAAAGAGVWGVTVAGGTITFETPTDAGVASATPIEILIGTNAVTSGAGANQIT
                     NPSATTSYEIAIGGTMQDSGHVRIAIVDQVTVSASVDTSLTFTVSGVVADQSVNGSPT
                     TTATDTTATTLPFGTLDPYGTTEVLAHDLSVSTNAANGYTVTVEQSGDLQSSTGATID
                     GFIDGNDTTTPSPWQAPAQIISNPDTWGHWGITSSDGSTTRTAEFGSDEWVSGSTTPV
                     VI"
BASE COUNT         1360 a         2163 c         2006 g         1263 t
ORIGIN      
        1 ggcgaggagg agttcttcaa tgcgctcggc gagccgatca aggagggtgg tctcgcgctc
       61 gagaagaagg tcgtctccga cctctcgcgg tacatggaga agctcatcgc gctcggggtc
      121 gacgtcagtt acaagaaatg agacattcgc actttgatat gcggcccctg cgccgtacca
      181 tactcatatg ctcctcccgg atctagcacg gctctcgacg cgcgcgttca agacgaacgc
      241 gatgcgtacg tggctcacca tcctcggaat gggcgtcggc acgggtgccg tcgtcgtcct
      301 cgtgggtttg ggcttcgggc tccagcagat tatcctcgag cagatcgtct tcggtgagac
      361 gctcctttcc ttgggtgtct cggcgacggg cgcgcacaac ctcgtcatcg acgacgaggc
      421 cgtcgcgaaa ttcaaggcga tggaccacgt attgaacgcg gcgccgctcg ctcgctttcc
      481 ggccctcgtc acgtacaagg ggctcacggg aaatatcttc ctccagggcg cggagcccgc
      541 gtacgtccgg tacgccggga tcaccgcgtc cgccggcgac gtgttcaccg atgatgatgc
      601 ggcgaacggg agcgaggtca tgctctcacc cgcgatcctg aagctcttcg gcgtcgaaga
      661 cccggagtcg ttcatcggcc aggaggtcac gttcaagata ctcgtccccg cgaacgacgg
      721 tacgccgaac gtcaatcagg tcaatatcga caagaagtac aaggtgaagg gcataacgaa
      781 ggaggagggc gtattgaacg tgctcatgat gctcccggag ctccgaaact acgtcggcgt
      841 cgatcagtac gaccgcgccc aagtgcgcgt cgacgcgagc gagaacctcg acccggtgac
      901 gaccgagctc gtgaagcaag gctaccgcgt caccgcgctc tcgaagacgg tcgagcaggc
      961 gtcgaagatc ttccagggca tccaggtgac gctcgcggtc ttcggcggta tcgcgctcat
     1021 cgtctcggcg atcggcatgt tcaatacgat gacggtcacg ctcctcgagc gtacgaagga
     1081 gatcggcatc atgcgaacga tcggcgcatc gccgcgcgac atcaagtacc tcttcgtctc
     1141 ggaatccatc atcgtgggtt tccttggggg tatctcgggg atagtcatgg gggtaaccct
     1201 gggattcatc gtgaacatca tgttgaacgt cgccgcgtca cagttcgggg gtaaggcggt
     1261 ccagctcttt caatttccgc tctggttcct cgctttcatc gccatattct cggccgccgt
     1321 cgggtacctc accggaatct tcccggcgcg ccgcgcttcg ctccttaacc cgctcgacgc
     1381 catccgctac gagtaggcgg ctgtgtataa ctctcgtgtt tccgccacgg ggtaccttac
     1441 aatacatcta acagcacata gtcaatatgt cctataacac agatcattta cgtacggcgg
     1501 gtaaagtcac tgtgtacacg accctggtag gcgtcatcgc gttcgccatc gttttcatat
     1561 tcaacctcgg cgagccgagc atcaaacagg ctgacgcgca gagccaggcg acgacgacgg
     1621 tgaccgtcct caatacgccg cctatctgga ccgcatcgac gacggagctc gtcgaatcgt
     1681 catctaataa tccgacgaac gccggtgaca cggtcagttg ggtcggtatc ggtacggact
     1741 cgaacggcga ggactactac ctcctcatct gcgatacgaa cgcgactccg acgccgaact
     1801 cgggcggagc gccgacgtgt agttcgggcg tgcagtgggc ggtctcgacg gcgacgttga
     1861 gcggcacgca ggcgctcgcc gcgacgacga cgtacgatgc gctctccgag gtgaacacgt
     1921 ggttcgcgtg gatctgcgac tcgaacgtca tctcgccgcg ttgtaacagc gattacacgc
     1981 agggtacgaa cgcgacgaac tcctccccgt tcgaggtgaa ccaccgtccg tccttcaccg
     2041 ccttcgtcga cgactcgccg aaggaccctg gtcagaccgt gacattcacg gcgaccgcat
     2101 cagacgcgga cgtctccggc gtcgccgaca cggtcttcct caccgtctgt gcgaccgcgg
     2161 gttactcgac gacgaccgat acctgtacgg gcacgacgct cgcgacctcg accatgttct
     2221 tctcgtcgaa cgcgacgtcg acctatacca tcgtcatccc gacgcaggat acgaactaca
     2281 gtgccttcgg ctacattatg gacaaccacg gcttcgaggc atcgggcggc gcacagggca
     2341 ccgactcgac gcttacggtc aataacgtcg cgccgacggt ctcacccgcg cttatctccc
     2401 tcgtccaacc gagcgtgacc gacatgttcc ttactgtcga ggcgggggag acgaccggct
     2461 tccagctctc gttcacgacc gctgacaaca acagctgtga tgcggtcggt ggcggaagcg
     2521 ccgatgagat caccggatac aacttgagca tctaccgctc cggcgtcggc tcgaccacct
     2581 gtacgaccgc ggcaggcgac tacgacgcga acaactgtta cccgagcggc ctcgcgacct
     2641 cgacgtggaa cctcacctgt accgcgtcgt cgacgacctg cagcggttcg tccgacacgg
     2701 acatgcagtg ggactgtacg ttcccgctct ggtacatcgc ggacccgacc gacggcacgg
     2761 cgacctcgac gcagtactcg accgaggatt gggtcgcgca ggtacagggt atcgacgacg
     2821 acgcggcgac tggtccgctc tccgaggcat cgtcaggcgt cgacgttcga agtctcctcg
     2881 cattcgcgct caatacgctc actatcccgt acggctccct cgaaccgggc cagcagaccg
     2941 accccctcaa tgcgacgacg accatctcgg cgaccggtaa cgtaggactc gataaggacg
     3001 tcgaaggtag ctccatgtgt acgacgtaca ccggctcgac gccctgtccc aattcagcga
     3061 cgtcgaccat tccggagaac atgcaggtcg tcgcgacctc gaccgtcgcg tacgcacagg
     3121 gtaccgccat ctcctcgacg accccgacct tcgtcgacat caacgtccat aagtcgatcg
     3181 cgacctcgac gcaggaacag ggaagcgcgt actggggcat caaagttccg gcgaccatca
     3241 ccttcgcggg tgactacacc ggacagaaca ccatcacggc tctcgtgagc gactcgagtc
     3301 agtggtgacc ctgacatcgc gcaggcgaac ccgtccttat gcagggacgg gttcttgctt
     3361 ttgtggcttc agtgttcgat aattaatgaa cttacgcata cgcgtttgtt tttatagtaa
     3421 atgaaaggac ttcgctcatt tctggcggtc atggcgctct tcgtcgtgtc cgcccagtac
     3481 gcgtacgcgg ggttcggcgt gacgccgccc tacgtgtcga acgtgagcct cacgcgtaac
     3541 tcggtctatg agcagacgat ctatctcgtc cggagcgatc cctcgaccga cctcaaggcg
     3601 acggtctccg tcgatgtccc cggcatcaat gattggttca cgattaacga ggggaacgag
     3661 ttcctccttc ccgcgggcga gcagaaggtc ccgatgacgg tccacatcgc ggtgcccgat
     3721 aatgccgatt tcaagaagta cgacggtaac attcgcatca agacgggacc acccgacgat
     3781 aaggtcgcag gcggcgccgt ctcgatatcg ctcggcgcgc agatagacgt gaacctcgtc
     3841 gtcatcgaca aggagatcaa ggacttcaag gttcgaaaga tcggcatctc cgacctcaac
     3901 gagggtcaca aggtcggatg gctctacttc ccgggcaaga tacgattcga gatgctcatc
     3961 gagaacatcg ggaacgttcc cgtcgcgccg tccgacgtcg tctttaggat atacgaccgc
     4021 tcgggcaacg ttctcctcga ggagacgcac aagaaggggc gcatcacgaa cgtcgatcct
     4081 ttcaagaccg agtctgtctt cgccgagctt ccgacgaaac ttcccgcggg cagttacctc
     4141 gcgcgcttcg agataaagaa cggcgacgag acgaagctct cgggcgaggt gaacgtgagc
     4201 atcctcccct atggtacgct tcaggccgcc ggattcggct tcctcggcct ctcgctcgcg
     4261 caccagctct ccatcgtcgt gcctattctc ttcctcctcg tcgcggcgat gctcatctac
     4321 gtgaggacac ggcgacggag gtgaacacgt atgatacgac gcttcgccta cacgctcccc
     4381 tggacggcac gcctcttcct tgtcgttctc tcgttcgtcg cgctcgcgct cacgcatacc
     4441 gcgctcgcgc agacggtcgt cggcacgagc atttcgacga cgacgctccg catctcgata
     4501 tgcggcgact cgctcgtgaa cgcgggcgag gagtgcgacg taccggggga caccggacag
     4561 tactcgacga cgatagccgg tcgacagtgc accgaccagt gcctctgggc accgtactgc
     4621 ggcgacggca tcctccagac gctctttagc gaggagtgcg acgacgggaa caatgatgac
     4681 ggcgattttt gttcggcgat atgcaaggta gaaccggcgg gctcgggcgg cggtcacgtc
     4741 ggcggcggcg ggagctcggg cggcggcggc tccgatacgc gcctcggcga cacgcaggtg
     4801 agcatcgtcg gtaaggcgta tccgaacgtc tcggtcacca tcctcgtaga cggcgacgtc
     4861 gtcgggacgg tgcgcacgaa ctcctccgcc gatttctcct tcaacaccga tacgaagccg
     4921 ggcaccgcgt cactctcgtt ctgggcgacc gactcgaagg gtacgcgctc cctcaccatc
     4981 tcgacgacct tcgacgtcac ccagggcgcc gtcacgaacg tgaacggcat cttcgtaccg
     5041 cccacgatcc gcacgtcgaa cgcgaacccg aaccccggcg agaccatcac gctctcgggt
     5101 cagaccatac cgaacgccac ggtcgaggtg ctcgtcgaca gcggcgcgaa gagactcacg
     5161 gcgacggcga acgcatcggg cgagtggtcg gtgcccttcg atacggggac cgtctccgcg
     5221 tcgacgcata ccgtgaaggc gcgcttcacg acggtaaacg gcgcgctccg caccgagagt
     5281 cagtacggca ccgcgctctc catcttcgtc ggcgtcgagg ggaagcctac gtcgagcgcc
     5341 gaccttaacc gcgacggcaa ggtgaacctc accgactttt ccatactcgt cttctggtgg
     5401 ggaacgcccg gcgggaactc gaacccgccc gcggacataa acggcaacgg gcgggtgagc
     5461 ctcgaggatt tctccattct cctcttcaat tggaccggct gacgagccac tttgtgtata
     5521 agtgcgcctt ccgcgaacgc gtggaagtac aatgaggtca cgggtactgg aggtgtcgcc
     5581 ctcgtacatg tgagggggcg acattggcgg ggatggtcct cgccgatgct tacagccgag
     5641 cattcaccgg taaacggaac gcgtccgcac gcggacgtaa gagtgtggag acttatatgt
     5701 ccgcacatcg catgccgtta agagcacggc atgcgaacct ctcgaaagag atggacgcgt
     5761 ggggttaata caacgtaaag cctatgattc acacggcgca taccgtgcga actcttcagg
     5821 ctgttgcgat gacggtcgga ctgtcgatac tcctctggag caccggcctt ccgacgatct
     5881 tccgcaccgc ggaagcggca tcaataacca acgcaagcga taccttgtcg acttcggcac
     5941 ccgggaatcc cgcgaaccat accatcgagt tcacgactcc gaacggtatg gcggtgggcg
     6001 agaccatcac ggtcacgttc ccaacgggcg cgaacgagtt cgtcatgggt tcggtaggcg
     6061 aggacgacgt cgatatcctc gtgaacggtt cctcatcctc gacggcggca gctgccggcg
     6121 ccggagtatg gggagtgacc gtcgcgggag gtacgatcac gttcgaaaca ccgacggatg
     6181 cgggagtcgc gtcagcgacg cccatcgaga tcctcatcgg tacgaacgcg gtaacgtccg
     6241 gcgcaggcgc gaaccagatc acgaacccgt ccgcgaccac gtcctacgag atagccatcg
     6301 gtgggacgat gcaggattcc gggcatgtcc gtatcgcgat cgtcgatcag gttacggttt
     6361 cagcgagcgt cgacacatcc ctcaccttca cggtctccgg cgtagtcgcc gaccagtcgg
     6421 tgaacggaag tccgacgacc actgctactg acacgaccgc gacaaccctc ccgttcggga
     6481 cgctcgatcc ctacgggacg acggaagtcc ttgcgcatga cctttctgtc tccacgaacg
     6541 ccgcgaacgg ctacacggtg acagtcgagc agtcaggtga tctgcagtcg tctactggcg
     6601 cgaccatcga tggattcatc gatgggaacg acaccacgac gccaagtccg tggcaggcgc
     6661 ccgcgcagat catatcgaac cccgatacgt ggggtcactg gggcatcacc tcgagtgacg
     6721 gctcgaccac gcgcacggcc gaattcggtt cggacgagtg ggtttcgggg agtacgacac
     6781 cggtagtcat ca
//
LOCUS       DASTSP010000086        10165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1557456, whole genome shotgun sequence.
ACCESSION   DASTSP010000086 DASTSP010000000
VERSION     DASTSP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557456"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /gene="rpsC"
                     /locus_tag="VFS75_03900"
     CDS             <1..359
                     /gene="rpsC"
                     /locus_tag="VFS75_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU4677831.1"
                     /translation="SIVAQMVVEGLEKRMPFRRVMKMTAEKVMANRDVKGVRIISSGR
                     LGGAEMARKEETKKGRIPLQTLRADIDFARETAVLPYGAIGVKVWIYRGDIFADRKVS
                     AETKSADAARRTRTQS"
     gene            375..809
                     /gene="rplP"
                     /locus_tag="VFS75_03905"
     CDS             375..809
                     /gene="rplP"
                     /locus_tag="VFS75_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4677832.1"
                     /translation="MLFPKKVKHRKWQVGRKHAVRLNAPETRGTSVSFGAYGLKAQSQ
                     ARVKSNQIEAARRVISRTLGKTGKVWIRIFPDKPVTKKPAEVGMGKGKGDPDHFIFEV
                     RPGRILFEIDGVTEAVAREALRKATAKLPVKAKVVTREETSV"
     gene            820..1005
                     /gene="rpmC"
                     /locus_tag="VFS75_03910"
     CDS             820..1005
                     /gene="rpmC"
                     /locus_tag="VFS75_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU4677833.1"
                     /translation="MSEIRKKNDADLVAFIGEKREELRKLRFGIGSGSRNTLAVRTLR
                     KELAQALTELGARNANA"
     gene            1025..1291
                     /gene="rpsQ"
                     /locus_tag="VFS75_03915"
     CDS             1025..1291
                     /gene="rpsQ"
                     /locus_tag="VFS75_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU4677834.1"
                     /translation="MATEHTTTENALPKGRVLRGKVVSTKMQDTIAVAVERYVMHPKY
                     KKYMRRTKKFLAHDKGNTAKEGDTVEIRETRPLSKRKHFELIKK"
     gene            1310..1681
                     /gene="rplN"
                     /locus_tag="VFS75_03920"
     CDS             1310..1681
                     /gene="rplN"
                     /locus_tag="VFS75_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU4677835.1"
                     /translation="MIQPQSIVKIADNSGGKIGRVFKVLGSSKKRYAELGELVVLSIQ
                     TAEPRKLVKKKDVCYGVVVRQVKPYRRKDGSYVSFDDNAVVLVEKDKGEPRANRVFGP
                     IPREVGESGYQKIISLAPEVV"
     gene            1698..2006
                     /gene="rplX"
                     /locus_tag="VFS75_03925"
     CDS             1698..2006
                     /gene="rplX"
                     /locus_tag="VFS75_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU4677836.1"
                     /translation="MKIRKGDTVVVTTGKDKGKEGKVIRAFPKKQAVLVEGIALVQRH
                     KKSTTRNSKGQIVEKPMPIPVANVAIRDEKTKKPARVGYTKDDKGNKVRVTRPSGAKV
                     "
     gene            2027..2611
                     /gene="rplE"
                     /locus_tag="VFS75_03930"
     CDS             2027..2611
                     /gene="rplE"
                     /locus_tag="VFS75_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4677837.1"
                     /translation="MAKTMKETLAGTFDALKEAHGYSNVMQTPRITKVIVSSGIGKIQ
                     DKQRRKLIEDRLARITGQKVSERPAKKSIASFKLRQGETIGYQVTLRGARMRDFLEKL
                     IHLALPQTRDFRGLPVTAIDDMGNYTLGIREHTIFPETADEEVKDVFGFAVTIVTTAK
                     SKKEAESLLRHIGLPLQAGGVEGKKAPKAKKKRK"
     gene            2933..3118
                     /locus_tag="VFS75_03935"
     CDS             2933..3118
                     /locus_tag="VFS75_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU4677838.1"
                     /translation="MARKSVLLRARRTPKYKSQVVRRCFKCGRKHGYMRDFDMCRICF
                     REAAHEGHIPGIKKSSW"
     gene            3112..3507
                     /gene="rpsH"
                     /locus_tag="VFS75_03940"
     CDS             3112..3507
                     /gene="rpsH"
                     /locus_tag="VFS75_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4677839.1"
                     /translation="MVGDPIGDFIIQLKNAGMVKKSSVSVPYSKLKHAVAEKLVEAGF
                     IASADKHGKKAKKTLDVTLKYDEKGAHQIQGVERISKPGRRLYRGVAEIFPVKFGRGK
                     RILSTPAGIVTGEEAKEKKVGGEELFIIW"
     gene            3518..4054
                     /gene="rplF"
                     /locus_tag="VFS75_03945"
     CDS             3518..4054
                     /gene="rplF"
                     /locus_tag="VFS75_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU4677840.1"
                     /translation="MSRLGKQPVQVPENVDVTFASGVLTVKGPKGELSRAIDEDIKVT
                     IDGKTVTFEKVKETKRSRALWGTFASHLHNMITGVTEGYAKKLEIEGVGYKADAQGDK
                     IKLSVGFSHPVMLQAPKGVTVSAEQGVITVSGIDKELVGQFAADIRGTKRPEPYKGKG
                     IHYVGEYIIRKQGKKGVA"
     gene            4076..4423
                     /gene="rplR"
                     /locus_tag="VFS75_03950"
     CDS             4076..4423
                     /gene="rplR"
                     /locus_tag="VFS75_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU4677841.1"
                     /translation="MITSHKRTQRTRRHARIRAKLAGTAARPRLAVFRSNRFIYAQLI
                     DDEAGKTLIGLDTRKAKGKTARDRAKELGLLVAEEAKKKGITTVVFDRGGFQYQGAIV
                     SLAEGAREGGLAF"
     gene            4441..5064
                     /locus_tag="VFS75_03955"
     CDS             4441..5064
                     /locus_tag="VFS75_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU4677842.1"
                     /translation="MQENDQQEAKSGTEGSAPVAAPVATATTDAPAGGAGGRFGRRGD
                     RNDRRNPRRARRGGRRDDRVRSEFDQKIISIRRVTRVMAGGRRFSFSVSIVIGNKKGK
                     VGVGIGKAADTQLAIEKAVRNARKHMIELPLDKNQRIPHDVHAKLSSSEVMMIPSPGR
                     GLVAGSSVRTVLELGGVKDVTAKIFSRSKNKLNNARAAIAALKKLKP"
     gene            5092..5541
                     /locus_tag="VFS75_03960"
     CDS             5092..5541
                     /locus_tag="VFS75_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HEU4677843.1"
                     /translation="MQLNELKPKAPRKDAKRIGRGGKRGKTSGKGGKGQTARAGGRPR
                     PEMRDIIKRLPKLRGHGKNRARTVRSEKARPVVINLGAIEAVYASGDTVSTGTLMAKG
                     LLASKKGKVPQVKLLGTGELTKKLTVAGVLCSASVRAKIEKAGGTIA"
     gene            5604..6875
                     /gene="secY"
                     /locus_tag="VFS75_03965"
     CDS             5604..6875
                     /gene="secY"
                     /locus_tag="VFS75_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU4677844.1"
                     /translation="MDTFLQKLKIIVSEKAIRDRILFMFGALIVFRVLTAVPIPGVDA
                     NVLEQFFSNNQFLGLLNVFSGGGLAKLSVAMLGVGPFITASIIMQLMTVMSPKLKSMY
                     SEEGETGRARFTQWSRLLTLPLAILQSFGFLTLLKSQGVIAGLSSFEFMVNIIIVTAG
                     SMLLVWLGELVTEYGFGNGVSFIIFSGIVAALPTHAAQLLFSFDASQLPLFAGFTLAA
                     LVIIFGVIVMTQAERQVPITHAKQVRGGKTYGGTTSYLPLRLNQAGVIPIIFALSIIL
                     FPQMVLNILSSFNVPNVAMLSEKVLAFFQNQPLYASVYFVLVIVFTFFYTAVTFDPDA
                     MSKNLQRSGAFIPGIRPGGHTAEYLGKVITRLTMAGAIFLGAIAVLPMLMQILTGVTT
                     LAIGGTALLIVVSVVIDLIRRVDSQVSLRQY"
     gene            complement(6919..7545)
                     /locus_tag="VFS75_03970"
     CDS             complement(6919..7545)
                     /locus_tag="VFS75_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEU4677845.1"
                     /translation="MERKHIITLSGKPGSGKSSTSDRIAEMLGYTRYSSGDYVRRIIQ
                     KQKITLQEFNRRAEAHPEIDHDIDEKLRELREQDDLVIDSRLGFYWIPESFKVYLDLD
                     PDVAVARIFKDANLNTLRSDAGEGGASMESVMDQVEERMRSERARFKKLYGVNPFSIE
                     HFDLIIDTARHSPDTVAMTIFDTYKKWLASSRWKQVVSRVPFGFSFKS"
     gene            complement(7571..8140)
                     /locus_tag="VFS75_03975"
     CDS             complement(7571..8140)
                     /locus_tag="VFS75_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4677846.1"
                     /translation="MKKHIITLGGLPGSGKSTMKRLLAERLGYKVFSTGDFVRNMAFE
                     RNLSLAELNDLIAKDKSLDHLIDDRLKHIEEKEDFYVVDSHLAFHFIPSGFSVFLDIT
                     PETAARRIFHDAQSPTRIKAGETMKTMSEAYSKTKSRVENHIDRYARHYGINPYDEKN
                     YSFVISTEESEPGEAVERIVAAYEAWLAA"
     gene            8223..8819
                     /locus_tag="VFS75_03980"
     CDS             8223..8819
                     /locus_tag="VFS75_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEU4677847.1"
                     /translation="MDPKTIIFIGPQGSGKGTQIERLARELRERDPKRRTVDIQTGRR
                     FRALAAKGEGYTEKSVHETLDSGVLQPLFLSVVLWGDAFHHHVDADCHLLIDGFPRTV
                     NEARVLESALSFYKRSPLTVINLDAPEDVVRERMTSRARPDDTPASIEARLRWYREET
                     MPVLAYYRSRADTVVLDCDGTKPIDEVQSEILRHLELI"
     gene            8821..9594
                     /gene="map"
                     /locus_tag="VFS75_03985"
     CDS             8821..9594
                     /gene="map"
                     /locus_tag="VFS75_03985"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU4677848.1"
                     /translation="MARIKTQDEIKLLRKGGKILARILRELAEYAKPGMSTADINDYA
                     LKLAEDYGVEPVLLGYHPEFAPIPYPAALCTSVNDTVQHGVPREDEILKEGDIINLDM
                     SIGYEGMIVDSGITVPIGKTDPESERLIKVTKEALLLGIKEARPGNRIGDISAAIEKH
                     VKAAGMSIVEVLCGHGVGHAVHEDPQIPNFGKAGTGEKIQVGNVFAIEPIVNLGGKDV
                     YFDDEGDGYSVYTVDGSRSCHFEHTVVVTEKGPEIVTVE"
     gene            9643..>10165
                     /locus_tag="VFS75_03990"
     CDS             9643..>10165
                     /locus_tag="VFS75_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU4677849.1"
                     /translation="MSTNRKPHQERTLVILKPDTVQRSLIGEVIARFERTGLKFTAMK
                     MLVPSREALLTHYNKDDAWFLRKGERIIGDLKAHGLPVDKDAMAYGRAIIDTIVDYMT
                     AAPVIAMIIEGNEAVKVVTKLVGTTEPSTSDVGTIRGDYTVDSYGHSSYENRAVRNLI
                     HCSESPEEAEREIK"
BASE COUNT         2109 a         3121 c         3131 g         1804 t
ORIGIN      
        1 cgtcgatcgt cgcgcagatg gtcgtcgaag gcctcgagaa gcgcatgccg tttcgccgcg
       61 taatgaagat gacggcggag aaggtcatgg cgaaccgcga cgtgaagggt gttcgcatca
      121 tctcatcggg tcgcctcggc ggcgccgaga tggcccgcaa ggaggagacg aagaagggac
      181 gcattccgct tcagaccttg cgcgcggaca tcgacttcgc tcgtgagacg gcggtcctcc
      241 cctatggcgc tattggcgtg aaggtatgga tctatcgcgg tgacatcttc gcggaccgta
      301 aggtgagcgc ggagacgaag tcggccgacg ccgctcgacg aacgcgcacg cagtcataat
      361 ctcacgatac gcttatgcta tttcctaaga aagtaaaaca tcgaaagtgg caggtcggcc
      421 gtaagcacgc ggtccgcctc aacgcccccg agacgcgcgg cacgtcggtc tctttcggtg
      481 cgtacggcct caaggcccag tcgcaggcgc gcgtgaagtc gaaccagatc gaggcggcgc
      541 gtcgcgtcat ctcgcgaacg ctcggcaaga caggcaaggt gtggattcgt atcttccccg
      601 acaagccggt cacgaagaag ccggcggagg tcggcatggg taaggggaag ggagaccccg
      661 accacttcat cttcgaggtg cgtccgggcc gcatcctctt cgagatcgac ggtgtgaccg
      721 aggcggtcgc tcgcgaagcg ctccgcaagg cgacggcgaa gctcccggtg aaggcgaagg
      781 tcgttacccg cgaagagacg tcggtataag gatatgcata tgtcagagat caggaagaag
      841 aacgatgcgg accttgtcgc cttcataggc gagaagcggg aagagcttcg aaagctccgc
      901 ttcggcatcg ggagcggcag ccgaaacacg ctcgccgtcc gcacccttag gaaggagctc
      961 gcgcaggcgc tcaccgagct cggcgcgcga aacgcgaacg cataatttag caatcgacaa
     1021 tactatggca accgaacaca caaccacaga gaacgcgctt ccgaaaggcc gagtccttcg
     1081 cgggaaggtc gtctctacga agatgcagga cacgatcgcc gtcgccgtcg agcgctacgt
     1141 tatgcatccg aagtacaaga agtacatgcg tcggacgaag aagttcctcg cccacgataa
     1201 gggcaatacg gcgaaggaag gggatacggt cgagatccgc gagacgcgcc cgctctcgaa
     1261 gcgaaagcac ttcgagctca tcaagaagta atcgcgtact accaaacata tgatccagcc
     1321 acaatccatc gtgaagatag cggacaactc gggcggtaag atcgggcgcg tcttcaaggt
     1381 gctcggcagt tcgaagaagc gctacgcgga actcggcgag ctcgtcgtcc tctccatcca
     1441 gaccgcggag ccgcgaaagc tcgtgaagaa gaaggacgtt tgctacggcg tcgtcgttcg
     1501 ccaggtgaag ccgtaccgcc gcaaggatgg ttcgtacgtg agcttcgacg acaacgcggt
     1561 cgtcctcgtc gagaaggaca agggtgagcc gcgcgcgaac cgcgtgttcg ggcccattcc
     1621 gcgcgaggtc ggcgagtccg gttatcagaa gatcatctcc ctcgcgcccg aggtcgtgta
     1681 atccctaaat ccattttatg aagatcagga aaggagacac cgtcgtcgtc acgacgggca
     1741 aagacaaggg taaggagggc aaggtcattc gcgccttccc gaagaagcag gcggtcctcg
     1801 tcgagggtat cgcgctcgta cagcgccaca agaagtcgac gacgcgaaac tcgaagggac
     1861 agatcgtcga gaagccgatg ccgatcccgg tcgcgaacgt cgccatccgc gacgagaaga
     1921 cgaagaagcc ggcgcgcgtc gggtatacga aggatgacaa gggaaataag gtccgcgtca
     1981 cgcgaccgag cggagcgaag gtttaataga agcatcacta ttacgtatgg ctaaaacgat
     2041 gaaggagaca ctcgcgggta cgttcgatgc gctcaaggag gcgcacggct attcgaacgt
     2101 gatgcagacc ccgcgcatca cgaaggtcat cgtctcgagc ggtatcggca agatacagga
     2161 caagcagcgg cggaagctca tcgaggaccg tctcgcccgc atcacgggac agaaggtgag
     2221 cgagcgtccg gcgaagaagt ccatcgcgtc gttcaagctc cgccagggcg agacgattgg
     2281 ctaccaggtg acgctccgcg gtgcgcgcat gcgcgacttc ctcgagaagc tcatccatct
     2341 cgcactcccg cagactcgcg acttccgcgg tctcccggtc acggcgatcg acgacatggg
     2401 gaactacacc ttgggcatcc gcgagcacac catcttcccg gagaccgccg acgaggaggt
     2461 gaaggacgtc ttcggattcg cggtcaccat cgtgacgacg gcgaagtcga agaaggaggc
     2521 cgagtcgctc ctccgccaca tcgggctccc gctccaggcg ggcggcgtcg agggtaagaa
     2581 ggccccgaag gcgaagaaga agcgaaagta gacacttttt gcactggaaa gccccgcact
     2641 gcggggtttt ttagtacgga gcggcgcgat acccgtggtg tcgctaaacc tttactttcg
     2701 aggtttgttt tgctatagtc tcggggttgc gatatacgca tactctcgtg ttttcatccc
     2761 gatagggaga cacaagacgc accgatcgac cattcccccg tgggaatcga cggctccgcg
     2821 attcttttcg agtaccgcgt acggaggtaa cggtcaccac atctaggtga gaccaccctt
     2881 caccattgca tacgttatca tccgcggggt aacccgctaa tctacacact acgtggcaag
     2941 aaaatcagta ctccttcgtg cgcgtcgtac tccgaagtac aagagtcagg tcgtccgccg
     3001 ctgtttcaag tgcggcagga agcatggata catgcgcgat ttcgacatgt gccgcatctg
     3061 cttccgcgag gcggcgcatg aaggacatat accaggcata aagaaatcat catggtagga
     3121 gacccgatcg gagatttcat catccagctc aagaacgccg gcatggtcaa gaagagctcg
     3181 gtgagcgttc cgtactcgaa gctgaagcac gcggtcgccg agaagctcgt cgaggccggt
     3241 ttcatcgcgt cggcggacaa gcacggcaag aaggcgaaga agaccctcga cgttaccctc
     3301 aagtacgacg agaagggcgc gcaccagatc cagggcgtcg agcgcatctc gaagccgggt
     3361 cgtcgcctct accgcggcgt cgcggagata ttccccgtga agttcggtcg cggtaagcgc
     3421 atcctctcga cccccgcagg catcgtgacc ggcgaggagg cgaaggagaa gaaggtcggc
     3481 ggcgaggagc tattcatcat ttggtaatca cacgcatatg agcagacttg gaaaacagcc
     3541 agtgcaggtt ccggagaacg tcgacgtgac gttcgcttcg ggcgtgctta cggtgaaggg
     3601 tccgaagggc gagctctcgc gcgctatcga cgaggacatc aaggtcacga tcgacgggaa
     3661 gaccgtcacg ttcgagaagg tgaaggagac gaagcgctcg cgcgccctct ggggtacgtt
     3721 cgcctcgcac ctccacaaca tgatcacggg cgtgaccgaa gggtacgcga agaagctcga
     3781 gatcgagggc gtcggctaca aggcggacgc gcagggcgac aagataaagc tctccgtcgg
     3841 gttctcgcac cccgtcatgc tccaggcccc gaagggcgtc acggtctcgg ccgagcaggg
     3901 cgtcatcacg gtctcgggca tcgacaagga gctcgtcggc cagttcgcgg cggacatccg
     3961 cggcacgaag cgcccggagc cgtacaaggg gaagggcatc cactacgtgg gcgagtacat
     4021 tatccgcaag cagggtaaga agggagtcgc gtaatcggcg ctctgacacg ataccatgat
     4081 cacatcacac aagaggactc agcgtacgcg tcggcacgcg cgcatccgcg cgaagctcgc
     4141 cggtaccgcc gctcgtccgc gactcgcggt gttccggagc aatcgcttca tctacgccca
     4201 gctcatcgac gatgaggcgg ggaagacgct catcggactc gacacgcgta aggcgaaggg
     4261 taagaccgcg cgcgatagag cgaaggagct cggtctcctc gtcgccgaag aggcgaagaa
     4321 gaaggggatc acgacggtgg tcttcgaccg cggcgggttc cagtaccagg gcgccatcgt
     4381 ctccctcgcc gagggtgcgc gcgaaggcgg cctcgcgttt taatcgaatc aatcacattc
     4441 atgcaagaaa acgatcagca ggaagctaaa agcggaaccg agggttccgc accggtagcg
     4501 gcccccgtgg cgacggctac caccgatgca cccgctggtg gcgcgggcgg ccgtttcggc
     4561 cgccgcggag accggaacga tcgccggaac ccgcgccgcg cacgccgggg aggaaggcgc
     4621 gacgatcgcg tccgctcgga gttcgaccag aagatcatca gcattcgtcg cgtgacgcgc
     4681 gtcatggccg gcggtcgccg attcagcttc agcgtctcca tcgtcatcgg taacaagaag
     4741 ggcaaggtcg gcgtcggtat cggcaaggcc gccgatacgc agctcgccat cgagaaggcg
     4801 gtccgcaatg cgcgaaagca catgatcgaa ctcccgctcg acaagaacca gcgcattccg
     4861 cacgacgtgc acgcgaagct ctcctcgagc gaggtcatga tgatcccgtc gccgggacgc
     4921 ggcctcgtcg cgggaagttc cgtccgtacc gtcctcgaac tcggcggcgt gaaggacgtc
     4981 accgcgaaga tattctcgcg ctcgaagaac aagcttaaca acgcgcgcgc ggctatcgcg
     5041 gcgctcaaga aactgaagcc ttaatcgctc cgctcataat aaattccagg tatgcaactc
     5101 aacgaactca aaccgaaggc acctcgaaag gacgcgaagc gtatcggccg aggtggtaag
     5161 cgcggcaaga cctccggtaa gggcgggaag ggtcagacgg cgcgagccgg tggccgtccg
     5221 cgtccggaga tgcgagacat catcaagcgg ctcccgaagc tccgcggtca cggtaagaac
     5281 cgagcgcgaa cggtccggag cgagaaggcg cgtccggtcg tcatcaacct cggcgcgatc
     5341 gaggcggtct acgcatcggg cgacacggtc tctaccggca cgctcatggc gaagggactc
     5401 ctcgcctcga agaagggcaa ggtaccccag gtgaagctcc tcggtacggg tgagctcacg
     5461 aagaagctca ccgtcgcggg cgttctctgc tcggcatcgg tgcgcgcgaa gatcgaaaag
     5521 gccggcggca ccatcgcgta gccccggctt ttgcctctgc gatatagtta cccacgtcta
     5581 atccgccgac attcggcacc agcatggaca ctttcctgca aaaactgaag atcatcgttt
     5641 ccgaaaaggc gatccgcgac cgcatcctct ttatgttcgg cgcgctcatc gtgttccgcg
     5701 tcctcacggc ggtgcccatt ccgggcgtcg acgcgaacgt cctcgagcag ttcttctcga
     5761 acaatcagtt cctcggactc ctcaacgtct tctcgggagg cggactcgcg aagctctcgg
     5821 tcgccatgct cggcgtcggc ccgttcatca ccgcgtcgat catcatgcag ctcatgacgg
     5881 tcatgtcgcc gaaactcaaa tccatgtact ccgaggaggg tgagaccggt cgcgcgcgct
     5941 tcacgcagtg gagtcgactc ctcacgcttc ccctcgccat tctccagtcg ttcggctttc
     6001 tcacgctcct caaatcgcag ggcgtcatcg ccggcctctc gagcttcgag ttcatggtga
     6061 acatcatcat cgtcacggcg ggctcgatgc tcctcgtgtg gctcggcgag ctcgtgaccg
     6121 agtacggttt cgggaacggc gtgtcgttca tcatcttctc gggtatcgtc gccgcgctcc
     6181 cgacccatgc ggcgcagctc ctcttcagct tcgatgcgtc ccaacttccc ctcttcgccg
     6241 ggttcacgct cgccgcgctc gtcatcatct tcggcgtcat cgtcatgacg caggcggagc
     6301 gacaggtgcc tatcacgcac gcgaagcagg tcaggggcgg caagacgtac ggcggtacga
     6361 cgagctacct accgcttcgc ctcaaccagg cgggcgtcat cccgatcatc ttcgcgctct
     6421 cgatcatcct cttcccgcag atggtcctca acatcctctc gtcgttcaac gtgccgaacg
     6481 tcgcgatgct ttcggagaag gtcctcgcgt tcttccagaa ccagccgctc tacgcgtccg
     6541 tgtacttcgt gctcgtcatc gtcttcacgt tcttctacac cgcggtcacg ttcgaccccg
     6601 atgcgatgtc gaagaatctc cagcgctcgg gtgcgttcat tccgggtatc cgccccgggg
     6661 gacacacggc agagtacctc ggcaaggtca tcacccgtct cacgatggcc ggcgcgatct
     6721 tcctcggcgc gatcgccgtc ctcccgatgc tcatgcagat cctcacgggg gtcacgactc
     6781 tcgcgatcgg cggtacggcg ctcctcatcg tcgtctcggt ggtcatcgac ctcatccgtc
     6841 gcgtcgattc gcaggtgtcg cttcgccagt actaacgggc acgaaaaaac gcgcacgaga
     6901 gtgcgcgttt tttcgtgcct aactcttgaa cgagaagccg aacggcacgc ggctcacgac
     6961 ctgtttccag cgcgatgacg cgagccactt cttgtacgta tcgaagatgg tcatggcgac
     7021 cgtgtcgggg ctatgccgcg cggtgtcgat gatgaggtcg aagtgctcta tcgagaaggg
     7081 gttcacgccg tagagcttct tgaagcgcgc gcgttcgctc cgcatgcgct cctcgacctg
     7141 gtccatgacg ctctccatcg acgcgccgcc ctcgcccgcg tcgcttcgga gcgtgttgag
     7201 gttcgcgtcc ttgaagatgc gtgcgacggc gacgtccggg tcgaggtcga ggtacacctt
     7261 gaacgattcc ggtatccagt agaagccgag tcgcgagtcg atgacgaggt cgtcttgctc
     7321 gcggagctcg cggagcttct cgtcgatgtc gtggtcgatc tccgggtgcg cctctgcgcg
     7381 gcggttgaac tcctggagcg ttatcttctg cttctggatg atgcgccgca cgtagtcgcc
     7441 ggaggagtat cgcgtgtagc cgagcatctc cgcgatgcgg tcggatgtcg atgacttgcc
     7501 gctcccgggc tttccggaga gggtgatgat gtgtttgcgt tccatacgtc ctctctagtg
     7561 tacacctctc ctacgccgcg agccacgcct cgtacgcggc gacgatgcgc tcgacggcct
     7621 cgccgggttc gctctcttcg gtgcttatga cgaacgagta gttcttctcg tcgtacggat
     7681 tgatgccgta gtggcgcgcg tagcggtcga tgtggttctc gacgcggctc ttcgtcttcg
     7741 agtaggcttc gctcatcgtc ttcatcgtct cgcccgcctt gatgcgcgtc ggcgactgcg
     7801 cgtcgtggaa gatgcgccgc gccgcggtct ccggggtgat gtcgaggaag acggagaaac
     7861 cgctcggaat gaagtggaag gcgaggtgtg agtcgacgac gtagaaatcc tccttctcct
     7921 cgatgtgctt gagtcggtcg tcgatgaggt ggtcgagcga tttgtccttc gcgatgaggt
     7981 cattgagctc cgcgagggaa agattccgct cgaacgccat gttccggacg aagtcgccgg
     8041 tcgagaacac cttgtacccg aggcgctcgg cgaggagccg cttcatcgtc gacttcccgc
     8101 ttccggggag gccgcctaag gtgatgatgt gctttttcat ggaaagtacg ataccatact
     8161 ctctcccgta tttcaggctt caacggagcg ccgtttcacg gtaggatagg ggaatcacgc
     8221 acatggaccc gaagaccatc attttcatag gcccgcaggg aagcggcaag ggaacgcaga
     8281 tcgagcgtct cgcgcgcgag cttcgcgagc gcgacccaaa gcgtcggacc gtcgacatcc
     8341 agacgggccg gcgctttcgg gcgctcgcgg caaaaggcga gggctatacc gagaagagcg
     8401 tccacgagac gctcgacagc ggcgtcctcc agcccctctt cctctcggtg gtcctctggg
     8461 gcgacgcgtt ccaccaccac gtcgacgccg actgccacct cctcatcgac ggattcccgc
     8521 gcacggtcaa cgaggcgcgc gtcctcgaga gcgcgctctc gttctacaag cgctcgcctc
     8581 ttaccgtcat caacctcgac gcgccggagg acgtcgtccg cgaacgcatg acctcgcgcg
     8641 ctcgccccga cgacacaccg gcctcgatcg aagcgcgtct ccgctggtac cgagaggaga
     8701 cgatgcccgt cctcgcgtac taccgctcac gtgccgatac cgtcgtcctc gattgtgacg
     8761 gcacgaaacc gatcgacgag gtccaatcgg agatactccg tcacctcgag ctcatctaat
     8821 atggcacgca tcaaaacaca ggacgagatc aagctcctcc ggaagggagg gaagatcctc
     8881 gcgcgcattc tccgcgagct cgccgagtac gcgaagccgg gcatgtcgac cgcggacata
     8941 aacgactacg cgctgaagct cgccgaggat tacggcgtcg agcccgtcct cctcggctac
     9001 cacccggagt tcgcgcccat tccgtacccc gcggcgctct gcacgtcagt gaacgacacc
     9061 gtccagcacg gcgtcccgcg cgaggacgag atcctcaaag agggcgacat catcaatctc
     9121 gacatgtcga tcggctacga gggtatgatc gtcgactccg gcatcacggt ccccatcggg
     9181 aaaaccgacc ccgaatccga gcggctcatc aaggtgacga aggaggccct ccttctcggc
     9241 atcaaggagg cgcgtcctgg gaaccgtatc ggcgacatca gcgccgcgat cgagaagcac
     9301 gtgaaagcgg ccggcatgag catcgtcgag gtcctctgcg gccacggcgt cggccacgcg
     9361 gtccacgagg acccgcagat accgaacttc ggcaaggcgg gtaccggaga gaagatacag
     9421 gtcgggaacg ttttcgccat cgaacccatc gtgaacttag gcggtaagga cgtctatttc
     9481 gacgacgagg gtgacggata tagcgtctac accgtcgacg ggtcgcgaag ctgccacttc
     9541 gagcatacgg tcgtcgtgac cgagaagggt cccgagatcg tcaccgtcga atgagccttg
     9601 cactgcggcc taagtagggt atactcagcc cattctattt atatgagcac gaatcgaaaa
     9661 ccgcaccagg agcgtacgct cgtcatactg aagccggaca cggtccagcg ttcgcttatc
     9721 ggcgaggtca tcgcgcgctt cgaacggacc ggtctcaagt tcaccgcgat gaagatgctc
     9781 gtcccgagcc gggaagcgct cctcacgcac tacaacaagg atgacgcatg gttccttcgg
     9841 aaaggggagc gcatcatcgg cgacctcaag gcgcacgggc ttccggtcga caaggacgcg
     9901 atggcgtacg gtcgcgccat catcgacacc atcgtcgact acatgaccgc ggcgccggtc
     9961 atcgcgatga tcatcgaggg gaacgaggcc gtgaaggtgg tcacgaagct cgtcggtacg
    10021 accgagccgt cgacgtccga cgtcggtacc atccgcggcg actacacggt cgactcgtac
    10081 ggtcactcgt cctacgagaa ccgcgcggtc cggaacctca ttcactgttc ggaatcaccg
    10141 gaggaggcgg agcgcgagat caagg
//
LOCUS       DASTSP010000087         5635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1334551, whole genome shotgun sequence.
ACCESSION   DASTSP010000087 DASTSP010000000
VERSION     DASTSP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334551"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..2007
                     /gene="thrS"
                     /locus_tag="VFS75_03995"
     CDS             247..2007
                     /gene="thrS"
                     /locus_tag="VFS75_03995"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU4677850.1"
                     /translation="MDQKLEQKRHTLAHLLALAVRKEFPYALPTIGPAIENGFYYDFD
                     FSGGAVPTEADLARIEERMRKYLPSWTAFTHKTVTPDEAREYFGRNAYKKEIVNELEY
                     AGEKITLYTVGEGESEFTDLCRGGHAEAPASDIDPASFKLASIAGAYWRGDEKNAMLT
                     RIYGYAFDTKEELEKYLADLEEAKRRDHRILGEKLDLFYFNEEIGKGLPIWLPRGNII
                     KEEIEKWAKETEAKWGYQRVTTPILTKGDLFLTSGHLPLYMDAMYAPIQIEDENYYIK
                     PMNCPLHHKTYAARPKSYRDLPLRLAEYGWCHRYEDSGSLFGLMRVRGMQMNDAHIYV
                     AKEDAIEEFVRVIKLHEYYYKVLGIHEYKMELALRDPNKMTKYHGNEQDWAEAELMTT
                     EAMERSGVPFTIVHEGAAFYGPKMDFQIRSSIGREFTASTNQLDLYAGKKFGLEYVDK
                     DGSRKTPVIIHRAPLGTHERFIAFLIEHFGGDFPLWLSPEQVRVIPVSDAHAPYAKKL
                     VEELTAIGIRASVDDSNESVGKRIRNAKLYKLPYFAVVGDKEVAAKSVTLESRKGETA
                     EMTLSNLGNRLLTEIETKAL"
     gene            complement(2062..4530)
                     /locus_tag="VFS75_04000"
     CDS             complement(2062..4530)
                     /locus_tag="VFS75_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677851.1"
                     /translation="MKYSIPGLEKYMNLVTRLFLVFVVLAAAYTVLALVVPKVNIHVS
                     MKGGDSIHSYADGGDGDGDGDGDGDGDGDGDGDGDGDGDGDGGAGVGDSGTGVGTGTG
                     DDGSGVGVGDAGDGCCGDGGTGGDDGTGTGVGDAGDGCCGDSGDGDSDTGGGGTTPSP
                     VCDVFYADPTSVVSAGNVTLHWATTDATDVSIDQGIGSVPLDGSTSMNVSFSRTFTLT
                     ASGPGGVVTCQAAVTVTQPPAAAPTCDAFTATPSSITGSGNVTLSWQTSNANTVSISG
                     VGSVPVDGQTAVFVNSTRTFTLTASGAGGTVTCQTAVTVTPPAANQPTCDAFTANPSS
                     LTNPGVVTLAWQTTNATGVSIDQGIGSVAADGSMPVSVNQTTTFTLTAVNQNGTVTCQ
                     TTVTVTPPSGNMPTCDAFTATPNAITAGQSATLAWQTTNATSASINQGIGVVAVDGSM
                     SVSPATSTTYVLTATGAGGTVICVTPITVNPVPPANLPVCDAFTASPSSMSGAGDVTL
                     SWQTTNANTVTIDQGVGSVAVDGSQAVRVSNDTTYTLTAENGNGTVTCQTSVDITGGG
                     GGGGGGGSSSPSCKLTASDTDIAAGEVVTLTWKNQRTNDIVIKDGDGKTIVDSRKDNS
                     IDEDAGSVDVYPAKRTKYTMTAIRGSRDRDCSVTVDVNGISLTSQRLQSGILLTSVPY
                     TGFEAGPVLTTIFYAAIVLWGLAIAYALVLRRRTVSSIIEAGKNEEDLESLEETHIPD
                     DVADEVVEVENPVTPLTALEERAHAQYALFSSEALRFIDGNEPDLERKHELLDQVIMR
                     AKARYPKENDWVVINKERLLALLA"
     gene            complement(4684..5433)
                     /locus_tag="VFS75_04005"
     CDS             complement(4684..5433)
                     /locus_tag="VFS75_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677852.1"
                     /translation="MRHAVMVVLAGLALAMLAGCGSFRIRNGFEIPRAQVRAEPAGRV
                     VHAACTHQSQAGCWEQRVLNFRFDPGFSGMAGLDRPYRSREERQWRKEELAPYLWREY
                     QAGRVSPSTPRSFYVCVYRALHIAPSQLSAYQVNGARNAYRHSVTEVPVEEVTATDSF
                     RCAIANGASPSQVFRIRADGREPIGASIQFPWAMFTDESAVLVCSGDRLTVYPNRITK
                     HEGLWVLPENLRWLRERNVDRTIEVFLSRDG"
BASE COUNT         1069 a         1796 c         1764 g         1006 t
ORIGIN      
        1 gaccgccgtc cccgtttgac aacgccggcg aagcgcatat catatgagga catcggagat
       61 tgcggcgcgt accgaacgcc ggtctatgac gcacgagagt gcctaatccg aacgagagag
      121 ccgcccggca taccgggtgg aagctcggtg gaaccgcggc tttgcttgcg caaagatcgt
      181 ccgggtgaaa acgcaatgct ttgtgttttt gcccggacgt ttttttattg gtaacgatac
      241 acttctatgg accagaaact cgaacagaag cgacacaccc tcgcacacct cctcgcgctc
      301 gccgtgcgga aggagttccc gtacgcgctc ccgaccatcg gccccgcgat agagaacggc
      361 ttctactatg atttcgattt ctcgggcggc gccgtaccga cggaagcgga cctcgcgcga
      421 atcgaagagc gaatgcgaaa gtacctaccg tcgtggaccg cgttcacaca taagacggta
      481 acacccgacg aggcgcgaga gtatttcggc cgaaacgcct acaagaagga gatcgtgaac
      541 gaactcgaat acgcgggaga gaagataacg ctttacacgg tcggcgaggg cgagtcggag
      601 ttcaccgacc tctgtcgcgg cggacacgcc gaagcgcccg cttcggacat cgaccccgct
      661 tcgttcaaac tcgcgtccat cgcgggcgcg tactggcgcg gcgacgagaa gaacgcgatg
      721 ctcactcgca tctacgggta cgcgttcgat acgaaggagg agctcgagaa atacctcgcg
      781 gacctcgagg aggcgaagcg gcgcgaccac cgcatcctcg gtgagaagct cgacctcttc
      841 tacttcaatg aggagatcgg caagggcctc cctatctggc ttccgcgggg caatatcata
      901 aaggaggaga tagagaagtg ggcgaaggag accgaggcga agtggggcta ccagcgcgtc
      961 acgacgccca tcctcacgaa gggtgacctt ttcctcacct cggggcacct ccccctctac
     1021 atggacgcga tgtacgcgcc catccagatc gaggacgaga actactacat caagccgatg
     1081 aactgcccgc tccatcacaa gacgtacgcg gcgcgaccga agagctatcg cgacctgccg
     1141 cttcgcctcg ccgagtacgg ctggtgccat cggtacgagg actcgggaag cctcttcgga
     1201 cttatgcgcg tccgcggcat gcagatgaac gacgcgcata tctacgtcgc gaaggaggac
     1261 gccatcgagg aattcgtccg cgtcatcaag ctccacgagt actactacaa ggtgctcggt
     1321 atacacgagt acaagatgga gctcgcgctc cgcgacccga ataagatgac caagtaccac
     1381 gggaacgagc aggattgggc ggaggcggaa ctcatgacga cggaggcgat ggagcgctcc
     1441 ggcgtgccgt tcaccatcgt ccatgagggc gcggcgttct acggaccgaa gatggatttc
     1501 cagatacggt cgtcgatcgg ccgcgagttc accgcgtcga cgaatcagct cgacctctac
     1561 gccgggaaga agttcggtct cgagtacgtc gacaaggacg gttcgcgaaa gacgcccgtc
     1621 atcatccacc gcgcgccgtt gggcacccac gagcgtttca tcgcgttttt gatcgagcac
     1681 ttcggcggcg acttcccgct ctggctctcg ccggagcagg ttcgcgtcat ccccgtctcg
     1741 gacgcgcacg cgccgtacgc gaagaagctc gtcgaggagc tcaccgcgat cggcatccgc
     1801 gcgtcggtcg atgacagcaa tgaatcggtc ggaaagcgca tccgaaacgc gaagctctat
     1861 aagctcccgt acttcgccgt cgtcggcgat aaggaggtcg ccgcgaagtc ggtcaccctc
     1921 gagtcgagga agggcgagac cgcggagatg accttgagca acctcgggaa ccgcctcctc
     1981 accgagatag agacgaaagc gctctagtga aaacacgaaa aaacacccac cgggtgtttt
     2041 ttcgtgctct atgcgagctc cctacgcgag gagcgcgagg agccgttctt tattgatgac
     2101 cacccagtcg ttctccttcg ggtagcgtgc cttcgcgcgc atgatgacct gatcgagaag
     2161 ctcgtgcttt cgctccaagt cgggctcgtt cccatcgatg aagcgaagtg cctccgaaga
     2221 gaaaagcgcg tactgcgcgt gcgcgcgctc ctcgagcgcc gttagcggcg tcacggggtt
     2281 ctcgacctca accacttcgt ccgcgacgtc gtcggggatg tgcgtctcct cgagcgactc
     2341 gaggtcttcc tcgttcttcc ccgcctcgat gatagaggag acggtgcgac gtctgaggac
     2401 gagcgcgtac gcgatcgcga gaccccaaag gacgattgcc gcatagaaga tggtggtgag
     2461 gaccggcccg gcttcgaagc ccgtgtacgg tacggacgtg aggaggatgc cgctctggag
     2521 acgctggctc gtgagcgaga tgccattgac gtccacggtg acggaacagt ctcggtcgcg
     2581 ggagccgcgg atcgcggtca tggtgtactt cgtccgcttc gcagggtaca cgtcgacgga
     2641 acctgcgtcc tcgtctatcg agttgtcctt cctgctgtcg acgatggtct tcccgtcgcc
     2701 gtcctttatg acgatatcgt tcgttcgctg gttcttccag gtgagcgtga cgacctcgcc
     2761 cgcggcgatg tcggtgtccg acgcggtgag cttacagctc ggcgagcttg acccgcctcc
     2821 cccaccgcct ccgccgccgc ccgtgatgtc gaccgacgtc tggcacgtca cggtgccgtt
     2881 cccgttctcc gcggtgagcg tgtacgtcgt gtcattgctt acgcggacgg cctgtgaacc
     2941 gtcgacggcg accgagccga cgccctggtc gatggtgacc gtgttcgcgt tcgtcgtctg
     3001 ccatgagagc gtgacgtcgc ccgcgccgct catcgaagag gggctcgcgg tgaacgcgtc
     3061 gcagaccggg agattcgccg ggggcaccgg gttaacggtg atcggcgtga cacaaatgac
     3121 cgtgccaccc gcgccagtcg cggtgagtac gtatgtggtc gacgtcgcgg gggacacgct
     3181 catcgagcca tcgacagcca cgacgccgat accttgattg atagatgcgg atgtcgcgtt
     3241 cgtcgtctgc cacgcgagcg ttgcgctctg accagccgta atggcgttcg gtgtcgcggt
     3301 gaacgcgtca catgtcggca tattccccga tggcggcgtc acggtgaccg tcgtctgaca
     3361 ggtcacggtg ccgttttggt tcaccgccgt gagcgtgaag gtcgtcgttt gattgacgct
     3421 caccggcatg gagccgtccg cggcgaccga accgataccc tggtcgatag agacacctgt
     3481 cgcgttcgtc gtctgccacg cgagcgtcac gactcccggg ttcgtgagcg acgatgggtt
     3541 cgcggtgaac gcgtcgcacg tcggctggtt cgcggccgga ggcgtcacgg taaccgccgt
     3601 ctgacacgtc acggtgccgc ccgcgccgct cgccgtgagc gtgaacgttc gcgtcgagtt
     3661 cacgaagacc gccgtttgac catcgacggg cacggagccg acgccgctta tggatacggt
     3721 gttcgcgtta gaggtctgcc acgaaagcgt cacgttcccc gaaccggtga tcgacgaagg
     3781 cgtcgcggtg aacgcgtcgc acgtcggtgc ggcagccgga ggctgggtca cggtgacggc
     3841 agcctgacac gtcactacgc cccctgggcc gctcgcggtg agcgtgaagg ttcgagagaa
     3901 actcacgttc atggaggtcg agccgtcgag cggcaccgac cctatcccct gatctatgga
     3961 aacatccgtt gcgtcggtcg tcgcccaatg gagcgtcacg ttccccgcac tgacgaccga
     4021 ggtcgggtcg gcatagaaga cgtcacagac ggggctcggc gtcgtaccgc cgcctccggt
     4081 atcactgtca ccgtcgccgc tatcgccgca acagccgtcg cccgcgtcgc cgacgcccgt
     4141 acccgtaccg tcgtcaccgc ccgtaccgcc atcgccacag cagccgtcgc ccgcgtcgcc
     4201 gactccgacg ccgcttccat cgtcgccggt acccgttcca acgccagtgc cgctgtcgcc
     4261 gacgcccgca ccgccgtcac cgtcgccatc accatcacca tcgccatcgc cgtcaccgtc
     4321 gccgtcgcca tcaccatcgc catcaccatc gccatcgccg ccgtccgcat agctatggat
     4381 gctgtcgccc cccttcatcg agacgtggat gttcaccttc ggaacgacga gcgcgaggac
     4441 cgtgtacgcg gccgcgagaa cgacgaacac gagaaagagc ctcgtgacga ggttcatgta
     4501 cttttcgagt cccggaatgc tgtacttcat gcgatgtgat ggttacgtga taagttcttt
     4561 gcgacggacg tcttacaaag aattgactat tgatgaatct catcaagagc gaattcccta
     4621 caagagaaaa agatacccgg ggaagcgcac agactgcgtg cgcttcccgg atacgggacg
     4681 gtgtcacccg tccctcgaaa ggaagacctc gatggtccga tccacgttcc gctcgcgaag
     4741 ccagcggagg ttctccggga ggacccagag accttcgtgc ttcgtgatgc ggttcgggta
     4801 gaccgtgaga cgatcgcccg agcacacgag gaccgcggac tcgtcggtga acatcgccca
     4861 ggggaactgg atcgatgcac cgatgggctc cctgccgtcc gcacgaatgc ggaagacctg
     4921 ggacggcgaa gcgccgttcg cgatcgcaca gcgaaacgag tcggtcgcgg tcacctcttc
     4981 gaccgggacc tccgtcacgc tgtggcggta cgcgttacgc gcgccgttca cctggtacgc
     5041 ggagagctgc gagggcgcga tgtgaagcgc gcggtacacg cagacgtaga agctccgcgg
     5101 cgtcgaggga gaaaccctac ccgcctggta ctcgcgccaa aggtacggcg cgagctcttc
     5161 cttccgccac tgccgttctt ccctgctccg gtacggccgg tcgagacctg ccatgccgga
     5221 gaatccgggg tcgaagcgga agttgaggac cctctgctcc cagcaaccag cttggctctg
     5281 gtgcgtgcac gccgcgtgaa cgacgcgccc ggccggttcg gcccgcacct gcgctcgcgg
     5341 gatctcgaac ccgttccgga ttcggaacga accgcaaccg gcgagcatcg cgagcgcgag
     5401 gcccgcaagc actaccatta ccgcgtgtcg catgctgacc tccctcgtgc gggccgggga
     5461 cagaccgtct tcggcatgtc ctcgacgggt tgatgttcac ggatgaccgt gacgtgctct
     5521 cgcggagccg gaacaggaag cggcgtcgta gcccgaagcg gcgggatgtc ccgacgcacg
     5581 ggcggtggcg gggcgctcgg aagcgatgct ttcggcttcg tgggcactcg ccggt
//
LOCUS       DASTSP010000088         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1780132, whole genome shotgun sequence.
ACCESSION   DASTSP010000088 DASTSP010000000
VERSION     DASTSP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780132"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VFS75_04010"
     CDS             <1..1150
                     /locus_tag="VFS75_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU4677853.1"
                     /translation="VGAFIVVVMVMVYEFATMRERATHLALHLAKDRVQYSHDIFSIL
                     FRNSSVPFIVVDEHGVIESTNPSAARLFHVELDSLDGLEVFHFIAAEDETKTALIPEY
                     FTHGKIVNDVEVVLHRPDGVDRYVLLSVFSFKDAKGKGKGLVTLIDVTKQKMVDKAKT
                     EFVSLASHQLRTPISGMKWNIELLLTAGRENLTPAQSGYVDKIAHGLERMDMLVGDFL
                     SVSKFELGTLSAQYTSFSAAQFLRTIYDEHDPLAKKKNVSLEPNLGKADGTISCDSHL
                     LHMIVSNLVSNAVKYTPEGGRVKFDAVLDPAHLTVTVADTGMGIPASEQDMLFSKLFR
                     ASNAKTQVTDGTGLGLYIVREAVKILKGDISFVSTEGVGTTFTVVLPK"
     gene            1195..1572
                     /locus_tag="VFS75_04015"
     CDS             1195..1572
                     /locus_tag="VFS75_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4677854.1"
                     /translation="METNAKILTVEDEKDLREAIVTALSGEGFNVLAAEDGEAGLATA
                     LKERPDLILLDITMPKMDGLSVLRELRKDDWGKNVKVIVMTAHDDMGKIAEVLEAGGN
                     EYVVKTDVSLAGIVAKAKEKLRA"
     gene            complement(1636..2040)
                     /locus_tag="VFS75_04020"
     CDS             complement(1636..2040)
                     /locus_tag="VFS75_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677855.1"
                     /translation="MEAENMNQNPAGSQQATPAPAAAPAAAPAAAPAATGEHKTLMAV
                     LAYVGILIIIPYIMAKNDPFVAFHMRQGFVLVIIEIAVYVLGMFAYALFPIWGLINLA
                     AFILSIVGIIHAVKGEEKELPLIGSFGHSVKI"
     gene            complement(2194..2583)
                     /locus_tag="VFS75_04025"
     CDS             complement(2194..2583)
                     /locus_tag="VFS75_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677856.1"
                     /translation="MDMLVRQTIAIPLDAHTYAPKTVHVSLGIEGPRPHSLEARRIKG
                     GIGVFCRTKRNEKKGNLLYRLYVSGIADITVYPTYLVPAEGQLALFAYESVDGRFVIE
                     ALGVGILAEAKAAAGKAFRIDGAGFVN"
BASE COUNT          576 a          864 c          920 g          563 t
ORIGIN      
        1 ggtcggcgcg ttcatcgtcg tcgtcatggt catggtgtac gagttcgcga cgatgcgcga
       61 gcgcgcgacg catctcgcgc tccacctcgc gaaggaccgc gttcagtact cgcatgacat
      121 cttttcgata ctcttccgga acagttccgt gccgttcatc gtcgtcgacg agcatggcgt
      181 catcgagtcg acgaacccct cggccgcgcg gctcttccac gtcgagctcg attcgctcga
      241 cggtctcgag gtctttcact tcatcgcggc cgaggacgag acgaagacgg cgctcattcc
      301 cgaatacttc acgcacggga agatcgtgaa cgacgtcgag gtcgtcctcc accgcccgga
      361 cggcgtcgac cgctacgtcc tcctctccgt cttctcgttc aaggacgcga agggaaaggg
      421 aaagggcctc gtcaccctca tcgacgtcac gaagcagaag atggtcgaca aggcgaagac
      481 ggagttcgtc tcgctcgcct cgcatcagct tcggacgccc atctccggca tgaagtggaa
      541 catcgagctt ctccttaccg cggggcgaga gaatctcacg ccggcgcagt ccggctacgt
      601 ggacaagatc gcccacggcc tcgagcgcat ggacatgctc gtcggcgact tcctctcggt
      661 ctcgaagttc gagctcggga cgctctcggc gcagtacacg tccttcagcg cggcgcagtt
      721 cctccgcacg atctacgatg agcacgaccc gctcgcgaag aagaagaacg tctctctcga
      781 gccgaatctc ggtaaggcgg acggcaccat ttcgtgcgac tcgcaccttc tccacatgat
      841 cgtctcgaat ctcgtctcga acgcggtgaa gtacacgccg gagggcggcc gcgtgaagtt
      901 cgatgcggtc ctcgacccgg cgcacctcac ggtgaccgtc gccgacacgg gcatgggcat
      961 tccggcgtcc gagcaggaca tgctcttctc gaagctcttc agggcatcga acgcgaagac
     1021 gcaggtgacc gacggtaccg gcctcggact ctacatcgtc cgggaggcgg tgaagatact
     1081 caaaggcgac atctccttcg tctcgaccga gggcgtgggc actaccttca ccgtcgtatt
     1141 gcccaaatag gggcggggcc gtatgcttag gaaataccga gtaacatttg agacatggaa
     1201 acgaacgcga aaatactcac cgtcgaagat gaaaaggacc tccgcgaggc catcgtgacc
     1261 gcgctctcgg gcgaggggtt taacgtcctt gccgcggagg acggcgaggc gggtctcgcg
     1321 accgcgctta aggagaggcc ggacctcatc ctcctcgata tcacgatgcc gaagatggac
     1381 gggctctcgg tcctccgcga actccggaag gacgactggg ggaagaacgt gaaggtcatc
     1441 gtcatgaccg cgcacgacga tatggggaag atcgccgagg tcctcgaggc gggcgggaac
     1501 gagtacgtcg tgaagaccga cgtctccctc gcgggcatcg tcgcgaaggc gaaggagaag
     1561 ctccgcgcgt agagagaagc gcacgaaaaa agctccgcgt gcggagcttt tttcgtgcgc
     1621 attcgcgctt caggcctaga tcttcacgct gtggccgaac gagccgatga gcggtagttc
     1681 cttctcctcg cccttcaccg cgtggatgat accgacgata gagaggatga acgcagcgag
     1741 gttgatgaga ccccagatcg ggaagagcgc gtacgcgaac atgccgagca cgtacaccgc
     1801 gatctcgatg atgacgagga cgaagccctg gcgcatatgg aacgcgacga acgggtcgtt
     1861 cttcgccatg atgtagggga tgatgataag gatgccgacg tacgcgagga ccgccatgag
     1921 ggtcttgtgc tcgcccgtcg ccgcgggtgc cgcggcgggt gctgccgccg gagcggccgc
     1981 cggagccggc gtcgcctgct gcgagcccgc agggttctgg ttcatgttct ctgcttccat
     2041 gtggtggtta tcgaaattaa gggttataag gttctggtac caattctagc ccattttctg
     2101 gctctgggaa gggcccgtgg ggatagggcg aacccgaaga aaaataaaga aaaaacccgg
     2161 cacacgaggt gccgggcgct ctacagtatg gattcagttc acgaatccgg ctccatcgat
     2221 gcggaatgcc ttgccggcgg cggccttcgc ctcggcgagg atgcccacgc cgagcgcctc
     2281 gatgacgaag cggccgtcga cgctctcgta ggcgaagagg gcgagctgcc cttccgccgg
     2341 tacgaggtac gtcgggtaga cggtgatgtc ggcgatgccc gatacgtaga gccggtagag
     2401 caggttccct ttcttctcgt tccgcttcgt gcggcagaag acgccgatgc cgcccttgat
     2461 gcgacgcgct tcaagggagt gggggcgcgg tccctcgatg ccgagcgaaa cgtgtaccgt
     2521 cttcggtgcg tacgtgtgcg cgtcgagcgg tattgcgatg gtctgcctca cgagcatgtc
     2581 catgcgtgcc tcccgtcgag tggtcctgcg ttttatggta caggaatcga gaggcgtggc
     2641 aaggtggggg cgattatggt ggattcggtg tccgcaaaat tgtggcgcag gataaggagg
     2701 attcggcgtc cgctcgtaat tcgcttacgc tcataacgag cacctgcccg agccctctgt
     2761 ttcggcgccc tacgtggggc gcctcatatc gagggctttt cgaatccagt atcgtccacc
     2821 taattgaata tgtagccgac ggctccatgc agcgaaacgg aaatgcggct tcaacactca
     2881 atttcttgtt cgacagcatg aggttcgaac ttgtactttg cac
//
LOCUS       DASTSP010000089         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1112073, whole genome shotgun sequence.
ACCESSION   DASTSP010000089 DASTSP010000000
VERSION     DASTSP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112073"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /gene="rplC"
                     /locus_tag="VFS75_04030"
     CDS             <1..211
                     /gene="rplC"
                     /locus_tag="VFS75_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU4677857.1"
                     /translation="KNTLRTPGSIGATGPQRVFKGTRMAGRMGGDRITVKNLKVIAVN
                     KEENILLVKGAVPGRKGTLVEVRSA"
     gene            215..940
                     /gene="rplD"
                     /locus_tag="VFS75_04035"
     CDS             215..940
                     /gene="rplD"
                     /locus_tag="VFS75_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU4677858.1"
                     /translation="MTTKKSQQTLEANIYSQEGKEVGSVKLPEQVFGAAWNPELVHEV
                     VVSMQGNARSNSAHTKNRGEVRGGGKKPWRQKGTGRARHGSTRSPIWVGGGQAHGPRN
                     ERDFGRKINKNARAKALASVLSKKLADDHVIFVDALTFGAPKTKDARTFISALGGIKG
                     KETIATKKANAALVLLSDRNAETELSFRNFGNVEVKQAKDVNPVELLNYKYVVVAKPD
                     AALKILEGRVSTKAARKVTAVTK"
     gene            942..1316
                     /locus_tag="VFS75_04040"
     CDS             942..1316
                     /locus_tag="VFS75_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU4677859.1"
                     /translation="MALFGKKKEKEAPVAVETKTAPAAKPSLTAPRSATSAIIRPRVT
                     EKAAILTEKNVYAFEVRRDATKHEIKDAVKALYNVTPLRVRVVNKEPRHFMSRMRGRD
                     TMEHGMRKAYVYLKKGDRIELV"
     gene            1334..>2189
                     /gene="rplB"
                     /locus_tag="VFS75_04045"
     CDS             1334..>2189
                     /gene="rplB"
                     /locus_tag="VFS75_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU4677860.1"
                     /translation="MKSYKPTSAGRRGMTVVSYKELLTNRRNAPRKGLTHGGRSTGGR
                     HSGGRTTNFYRGGGNKRTYRVIDFRYDKKDIPAVVESIEYDPFRSGFIALVLYKDGER
                     RYVLAPKDMKKGDEFVVSETAKARNGNRMPLGNVPVGTFVFAIELTPGAGARIARSAG
                     GYAEVVAHDAGYTLLKMPSSEVRRVISTAWATIGQVSNEGHRLVNLGKAGRARYRGRR
                     PKTRGSARNAVDHPHGGGEGRAPRGHRRARTIQGRPTGKGQKTRRPKKHSNTFVVSRR
                     KPGRLMVPR"
BASE COUNT          489 a          662 c          721 g          317 t
ORIGIN      
        1 gaagaacacg ctccgcacgc cgggttctat cggcgcgacg ggcccccagc gcgtcttcaa
       61 gggaacgcgg atggcgggac gcatgggcgg cgaccgcatc acggtcaaga atcttaaggt
      121 catcgcggtc aacaaggagg agaacatcct cctcgtgaag ggagcggtac ccggtcgaaa
      181 gggaacgtta gtggaggttc gtagcgcata gcgtatgact accaagaaat cacagcagac
      241 actcgaggcg aacatctatt cgcaggaagg aaaggaggtt gggtccgtga agctcccgga
      301 gcaggtcttc ggtgcggcat ggaatccaga gctcgttcac gaggtcgtcg tctccatgca
      361 ggggaacgcg cgctcgaact ccgcgcacac gaagaatcgc ggtgaggtgc gaggcggcgg
      421 caagaagccg tggcgacaga agggcaccgg ccgcgcgcga catggctcta cgcgctcacc
      481 aatttgggtg ggcggcggtc aggcgcacgg cccgcgaaac gagcgagact tcggtcggaa
      541 gatcaacaag aacgcgcgcg cgaaggcgct cgcgagcgtc ctctcgaaga agctcgcaga
      601 cgaccacgtc atcttcgtcg acgcgctcac gtttggcgcg ccgaagacga aggacgcacg
      661 cacgttcata agcgcgcttg gcggcatcaa ggggaaggag acgatcgcga cgaagaaagc
      721 gaacgccgct ctcgtccttc tttccgaccg aaacgccgag acggagctct cgttccgcaa
      781 cttcgggaac gtcgaggtga agcaggcgaa ggacgtgaat ccggtcgaat tactgaacta
      841 taagtacgtc gtggtcgcaa agccggatgc ggcgctcaag atcctcgagg gtcgcgtgag
      901 cacgaaggcg gcacgtaagg tcacggcggt aacgaagtaa catggcatta tttggaaaga
      961 agaaggagaa ggaggctccc gtagcggtcg agacgaagac cgcgcctgcg gcgaagccgt
     1021 ctctcacggc gcctcgctcc gcgacgtcgg cgatcattcg cccgcgcgtc acggagaagg
     1081 cggccatcct caccgagaag aacgtctacg cgttcgaggt gcgaagggac gcgacgaagc
     1141 acgagatcaa ggatgcggtg aaggcgctct acaacgtgac gccgcttcgc gttcgcgtcg
     1201 tcaataaaga gccgcggcac ttcatgtcgc gcatgcgcgg ccgggatacc atggagcacg
     1261 gcatgcgaaa ggcgtacgtc tacctcaaga agggagaccg tatcgagctg gtgtaatcgg
     1321 ataagcaact atcatgaaat cctacaaacc aacatcagca ggacgccgcg gcatgaccgt
     1381 cgtgtcctac aaggaacttc tcacgaaccg ccggaacgcg ccccgaaagg gactcacgca
     1441 cggcggacgc agtacgggcg gacgccacag cggcggccgc acgacgaact tctatcgcgg
     1501 cggcggcaat aagcggacgt accgcgtcat cgacttccgc tacgacaaga aggatatccc
     1561 cgcggtcgtc gagtcgatcg agtacgaccc gttccgctcg ggattcatcg cgctcgttct
     1621 ctacaaggac ggcgagcgtc ggtacgtcct cgcgccgaag gacatgaaga agggtgacga
     1681 gttcgtcgtc tcggagaccg cgaaggctcg aaacgggaac cgtatgccct tgggcaacgt
     1741 ccccgtcggc accttcgtct tcgcgatcga gctcacgccc ggcgcgggtg cgcgcatcgc
     1801 gcgctccgcg ggcgggtacg ccgaggtcgt tgcgcacgac gcgggctaca cgctcctcaa
     1861 gatgccgtcg tcggaggtca gacgagtcat ctcgaccgca tgggcgacca tcggtcaggt
     1921 ctccaatgag ggtcaccgac tcgtgaacct cggtaaggcg ggccgcgcgc gataccgcgg
     1981 acgccggccg aagacccgtg gatcggctcg aaacgccgtc gatcacccgc acggaggagg
     2041 cgagggccgc gcaccgcgcg gacatcgccg ggcacgcacc attcaggggc gtccgaccgg
     2101 aaagggccag aagacgcgcc gaccgaagaa gcactccaat accttcgtcg tttcccgccg
     2161 aaagccggga cgactcatgg ttcctcgcg
//
LOCUS       DASTSP010000090         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U2283
            k141_1114389, whole genome shotgun sequence.
ACCESSION   DASTSP010000090 DASTSP010000000
VERSION     DASTSP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960747
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 01:54:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 818
            CDSs (total)                      :: 782
            Genes (coding)                    :: 781
            CDSs (with protein)               :: 781
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114389"
                     /isolate="SMAG_U2283"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFS75_04050"
     CDS             <1..221
                     /locus_tag="VFS75_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU4677861.1"
                     /translation="FVERLAAERGLTGWVRNNDDGSVEAVLQGLPDDLKACIEEMHRG
                     SPLARVESVAVDWRTPEKQFDEFKVISS"
     gene            224..907
                     /gene="uppS"
                     /locus_tag="VFS75_04055"
     CDS             224..907
                     /gene="uppS"
                     /locus_tag="VFS75_04055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU4677862.1"
                     /translation="MEERPACVGFIMDGNRRWARERGLPTLEGHRAGYETLKSLVRTA
                     RRERIPHVVCYAFSTENWRRPEDEVSYLMDLMGEALAELAESMRGEDGAVNVRVIGDE
                     SRLTNALRDGIRRAEAKNESENPLTVWVALSYGGRAEIVEAANRVRSEDGRLDEASFE
                     RFLWSGEMPDPDLIVRTGGEKRLSNFLLWKASYSELFFSDTLWPDFGDEEFRSILDEY
                     GKRQRRRGA"
     gene            876..1631
                     /locus_tag="VFS75_04060"
     CDS             876..1631
                     /locus_tag="VFS75_04060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4677863.1"
                     /translation="MGKGSDDAAREIAVLYEDADMLIVDKPSGMMVHDDGRAGGDTVV
                     DWFLARHPEAEGVGEPGKTPEGKPLQRSGVVHRLDRETSGVLALAKNADAHAALKRQF
                     HDHLVRKEYRAVVYGTMKEKWGTVDRPIGRSKRDFRLRSAQRGASGTLRDAVTDWELI
                     GQSSTHAYLKILPKTGRTHQIRVHLKAIGRPIVHDTLYAPNDLLMGDNLGFTRLALHA
                     YSLKFTTPAGEEKVVIAPLPLDFEVASTAIATA"
     gene            1697..2212
                     /gene="rplS"
                     /locus_tag="VFS75_04065"
     CDS             1697..2212
                     /gene="rplS"
                     /locus_tag="VFS75_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU4677864.1"
                     /translation="MNTIAGITISSVNMEDRKNLGIKAGDTVRVHQKIQDKGKTRLQV
                     FEGIVLARKHGDEAGATFTVRKVVDGIGVEKIFPLYSPLIDKIEILRRSTVRRAKLYY
                     IREKVAREIKRQMRRMRLMDMASESEAEAAERARVAEEEAKAKADADAKAAEAAAAPA
                     EEVAPEGETKA"
     gene            2316..2399
                     /locus_tag="VFS75_04070"
     tRNA            2316..2399
                     /locus_tag="VFS75_04070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2350..2352,aa:Leu,seq:gag)
     gene            complement(2491..3018)
                     /locus_tag="VFS75_04075"
     CDS             complement(2491..3018)
                     /locus_tag="VFS75_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4677865.1"
                     /translation="MTIRSAMRGFTLIELLVVVAIIGLLASMITAALASARVKGQYAA
                     AQSELLEFRKSAVIAQGESRKRLQDITGSGCSDCVCRNRDIRNVATTDACYTQWLNVL
                     TRIQSNSNGIVTKIDRMTRDPWGSPYGLDENERESGPTYCVMDNIRSAGPNGIINDGD
                     DIAYNIPFSTDCPLP"
     gene            complement(3022..3282)
                     /locus_tag="VFS75_04080"
     CDS             complement(3022..3282)
                     /locus_tag="VFS75_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4677866.1"
                     /translation="MSDNLMFQEYEANTHYRPRRVPFMVRFLMEHSGGRVTTETQANT
                     LLVLFASAALLLAVYFFHAATEPPHEPPAGFRGEITGVPVNP"
     gene            3346..3771
                     /locus_tag="VFS75_04085"
     CDS             3346..3771
                     /locus_tag="VFS75_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4677867.1"
                     /translation="MTEKSYGIIPFHHGKDGLEVFLIHQYGSAGDTLWTFPKGRAEEG
                     ESPIDTARREFSEETGMTLAAVDEKSSVATSYSFLRKGEPVDKTSTYFIGFVDVTDFS
                     VQPEEVKEAGWFTIPDARLRLTFPDYKKLLDEALTYLDN"
     gene            3805..3879
                     /locus_tag="VFS75_04090"
     tRNA            3805..3879
                     /locus_tag="VFS75_04090"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3838..3840,aa:His,seq:gtg)
BASE COUNT          814 a         1142 c         1257 g          755 t
ORIGIN      
        1 acttcgtcga acggctcgcc gccgaacgag gactcaccgg ctgggtacgg aacaacgacg
       61 acggttccgt cgaggcggtt cttcagggac ttcccgatga tctcaaagcg tgcatcgagg
      121 agatgcaccg cggctctccg ctcgcacgcg tcgagtcggt tgccgtcgac tggcgaacgc
      181 cggagaaaca attcgacgag ttcaaagtta tatcttcatg agtatggaag agagaccagc
      241 ctgtgtcgga ttcatcatgg acggcaatcg ccgttgggcg cgcgagcgcg gacttccgac
      301 cctcgaggga caccgcgcgg gctatgagac gctgaagagt ctcgtgcgaa cggcgcggcg
      361 agagcgcatc ccgcacgtcg tctgctatgc gttctcgacg gagaactggc ggcgacccga
      421 ggatgaggtc tcgtacctca tggacctcat gggagaggcg ctcgccgaac tcgccgaatc
      481 gatgcgcggc gaggacggcg cggtgaacgt gcgggtcatc ggcgatgagt cgcgcctcac
      541 caacgcgctc cgcgacggca tccgaagggc ggaggcgaag aacgagtcgg agaacccgct
      601 cacggtgtgg gtcgcgctct cgtacggcgg ccgcgcggag atcgtcgagg cggcgaaccg
      661 cgtgcggagc gaagacggtc gcctcgacga ggcgtccttc gagcgcttcc tctggtcggg
      721 cgagatgccg gaccccgacc tcatcgtgcg caccggcggc gagaagcggc tctcgaactt
      781 cctcctctgg aaggcctcgt acagcgagct tttcttctcg gacacgctct ggcccgactt
      841 tggagacgag gaatttcgga gtatcctaga cgaatatggg aaaaggcagc gacgacgcgg
      901 cgcgtgagat agcggtactc tacgaggacg ccgacatgct catcgtcgat aaaccgtcgg
      961 gcatgatggt ccacgacgat gggcgagcgg gcggcgacac ggtcgtcgat tggttcctcg
     1021 cgcgccatcc ggaagcggag ggcgtcggcg aaccggggaa gacccccgag gggaagccgc
     1081 ttcagcgctc gggcgtggtt caccgcctcg accgcgagac ctcgggtgtc ctcgccctcg
     1141 cgaagaacgc cgacgcgcac gcggcgctga agcggcagtt ccacgaccac ctcgtgcgga
     1201 aggagtaccg cgcggtcgtc tatgggacga tgaaggagaa gtgggggacg gtcgaccgtc
     1261 cgataggacg aagcaagcgg gattttcgcc tccgctccgc gcagcgaggc gcctcgggga
     1321 cgcttcgaga cgcggttacg gactgggagc tcatcggaca gtcgtcgacg cacgcgtacc
     1381 tcaaaatact tccgaagacg gggcggacgc accagatacg cgtccatttg aaggcgatcg
     1441 gccggcccat cgtccatgac acgctctatg cgcccaacga cctccttatg ggcgacaacc
     1501 tcggtttcac gcgccttgcg ctccacgcgt acagccttaa gttcacgact cctgcgggcg
     1561 aggagaaggt cgttatcgcg ccgcttcccc tcgacttcga ggtcgcgtcg accgctattg
     1621 cgaccgcata attcctgtgt tagagtacag cattcttttt tagagctcct caatggagag
     1681 cgcgtaaaca atacatatga acacaatcgc aggcatcacc atctcttccg tgaacatgga
     1741 agaccgaaag aatctcggca tcaaggccgg cgatacggta cgtgtccacc agaagattca
     1801 ggataaggga aagacccgac tccaggtctt cgagggcatc gtcctcgcgc gcaagcacgg
     1861 cgacgaggct ggtgcgacgt tcaccgttcg aaaggtggtg gatggtatcg gcgtcgagaa
     1921 gatattcccc ctctactcgc ccctcatcga caagatcgag atccttcgtc ggagcacggt
     1981 tcgtcgcgcg aagctctact acatccgcga gaaggtcgct cgagagatca agcgccagat
     2041 gcgccgcatg cgcctcatgg atatggcatc ggagtccgag gcggaggccg cggagcgcgc
     2101 tcgcgtagcg gaggaggagg cgaaggcgaa ggcggatgcc gacgcgaagg ccgcggaagc
     2161 ggcagcggct ccggccgagg aggtagcgcc cgagggagag acgaaggcgt aacgcacact
     2221 atttcgtatt cgagaaaagg cgcgcacgac gcgccttttc atttgcaatt gcgccaaaaa
     2281 tccttagaat ggtggcactc atttcgatga gcgtcgctca ggtggcgaaa ttggtagacg
     2341 cactaccttg aggtggtagc gcccgcaagg gcatgctggt tcaaatccag tcctgagcac
     2401 aaaacgtaaa gaaaaccatc cctgcggatg gttttctcgt attgtggaag cgactgggag
     2461 cctcttcgag cgttatcgcg ccgacgtggt ctacggtagc ggacaatccg tgctgaacgg
     2521 tatgttgtac gcgatgtcat cgccatcgtt gatgatgccg tttggccccg ctgaacgtat
     2581 gttgtccatg acgcagtatg tgggacccga ttcgcgctcg ttctcgtcga gtccgtacgg
     2641 gcttccccag ggatcgcgcg tcatgcggtc gatcttcgtc acgatgccgt tcgagttgct
     2701 ctgtatccgc gtgagcacat tgagccattg cgtatagcag gcatcggtcg tcgcgacgtt
     2761 ccgaatgtcc cggtttctgc agacgcagtc actgcatccc gaaccggtga tgtcttggag
     2821 gcgcttccgg ctctcgccct gcgcgataac cgcggatttc cgaaactcaa gcagttccga
     2881 ttgcgcggcc gcatactggc cttttacgcg tgcggaggcg agcgccgccg ttatcatgct
     2941 cgcgagtagc ccgataatgg cgacgacgac caaaagttcg atgagcgtga agccgcgcat
     3001 ggcggagcgg atggtcatgc gttatgggtt aaccggtacg ccggtgattt ctccgcggaa
     3061 tcccgcgggc ggttcatggg gcggttccgt cgccgcatga aagaaataca cggcgagcaa
     3121 gagtgcggcc gacgcgaaga ggacgaggag ggtattcgcc tgcgtctccg tcgtcacgcg
     3181 gccaccagaa tgctccatga ggaatcggac catgaaggga acccgccgcg ggcggtagtg
     3241 cgtgttcgcc tcgtactctt ggaacatgag attgtcgctc atggctcgta ctctgattat
     3301 actactcccc gcgaaccgcc gagaataccg gtgtatactg gtcccatgac ggaaaagtcc
     3361 tacggaatca taccgtttca tcacgggaag gacggtctcg aggtcttcct cattcaccag
     3421 tatgggagcg cgggcgacac gctctggacc tttccaaagg ggcgggccga ggaaggggag
     3481 agtcccattg ataccgcaag gcgcgagttc tccgaggaga ccggcatgac gctcgccgcg
     3541 gtcgacgaga agtcttcggt cgcgacgtcg tattcctttc ttcgaaaggg agagccggtc
     3601 gacaagacgt cgacgtactt catcggcttc gtcgacgtga cggacttcag cgtacagccg
     3661 gaggaggtga aggaggcggg ttggttcact atccccgacg cgcgactccg gctcacgttc
     3721 cccgattaca aaaaacttct cgatgaggcc ctcacttatc tcgacaattg aggtgaaacc
     3781 cgctataact atgcggtcca catggcggct atggtgtaac ggttaacact gaggtttgtg
     3841 gagccttcga tctgggttcg attcccagta gccgccccaa gtgagaatga gagaagcaat
     3901 tttctcattc cgcgtttcag tagaaagctt tttgtccagt tctaaccgat caaattcggc
     3961 ttttagtg
//
