LOCUS       DASTUV010000001         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_428169, whole genome shotgun sequence.
ACCESSION   DASTUV010000001 DASTUV010000000
VERSION     DASTUV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428169"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="VFT03_00005"
     CDS             complement(<1..1847)
                     /locus_tag="VFT03_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEU4846550.1"
                     /translation="MAEIFENPPQIEPNYEPLGAAELVDHDGRSVRLKAGSTIVEVSA
                     LAEDIFRVGAFPNGSTPRYDSEAIAKQDWEPVEVSMESSDGKLTLSTSAATAHVSLDP
                     LRIFFSDVGGRVLAADEEELGMGFSRRPEADVFTEPLDPSPRLYKRREEGERYFGCGE
                     RTSGLEKTGSHQIFWNVDPPLGHTAAFNNLYTSIPFSFSMTSGRAHGLLFDNPGRVEF
                     DLALEDEGRAYYGAEGGNLVYYVFCGPTPAEVLSSYTELTGRTPMPPLWSLGNQQCRY
                     SYMDEDEVREVAHNFRERDIPCDVIYLDIHYMDGYRVFTWDKERFPDPEKLTSDLAEQ
                     GFRVVTIVDPGVKVDENYPVYTEGRARDLFCKTGTGEEYHNAVWPGICAFPDFSSSET
                     REWWGENHKALLDRGVAGIWCDMNEPSLFIPRQSTMPGDVVHPGDDDPRFHAQIHNAY
                     GSLMARSVREGLLKLRPDERPFVITRAGYAGLQRHAMQWTGDNSSWWEHLWMSMPQLQ
                     NLGLSGVAWAGVDVGGFWGDTNAELLARWAEFGAFQPFCRNHTAIGTRRQEPWAFGEP
                     CESVYRKMMKLRQRLLPYLYSLFEMCHRTGFPIMRPLLFEYPEDETTYTA"
BASE COUNT          333 a          663 c          684 g          382 t
ORIGIN      
        1 gcggtgtaag tcgtctcatc ctcaggatac tcgaagagga gaggacgcat aatcgggaat
       61 ccggtcctgt gacacatctc gaagagggag tagaggtagg ggaggaggcg ctgccggagc
      121 ttcatcatct tgcggtacac cgactcgcag ggctcgccaa aggcccaggg ctcctggcgg
      181 cgggtgccta tggcggtgtg gttacggcag aaaggctgga aggcgccgaa ctctgcccac
      241 cgggccagta gctcggcgtt cgtgtcgccc cagaagccgc cgacgtccac cccggcccag
      301 gcgacgcccg agaggccgag gttctggagc tggggcatgc tcatccagag gtgctcccac
      361 caggaggagt tatcccccgt ccactgcatc gcgtggcgct gcagaccggc gtagccggcg
      421 cgggtgatca cgaacggacg ctcgtcgggc ctgagcttca gcaggccctc gcgcaccgag
      481 cgggccatga gggagccgta agcgttgtgg atctgggcgt ggaacctggg gtcgtcgtcg
      541 cccggatgca ccacgtcccc tggcatagtg gactgcctgg ggatgaagag cgacggctcg
      601 ttcatgtcgc accagatccc cgcgacaccc ctgtccagga gcgctttgtg gttctcgccc
      661 caccactcgc gggtttcgga gctcgagaag tccgggaacg cgcagatccc cggccacacg
      721 gcattgtggt actcctcccc cgtgcccgtc ttgcagaaca ggtcacgggc ccgcccttcc
      781 gtgtagacgg ggtagttctc gtccaccttc acgccggggt ccactatggt cacgacgcgg
      841 aagccctgtt ccgccaggtc ggaggtgagc ttctcgggat ctgggaaacg ttctttatcc
      901 caggtgaaga ctcggtagcc gtccatgtag tggatgtcga ggtagataac gtcgcacggg
      961 atgtcccgct cccggaagtt gtgcgctacc tcgcgcacct cgtcctcgtc catgtagctg
     1021 tagcggcact gctggttacc cagggaccag aggggcggca ttggagtgcg gcccgtcagc
     1081 tcggtgtagc tggacagcac ctctgccggg gtcgggccgc agaagacgta gtagacgagg
     1141 ttgccgccct cggctccgta gtaggcgcgg ccctcgtcct cgagcgccag gtcgaactcg
     1201 acgcgccccg ggttgtcgaa gaggaggccg tgagccctgc cgctcgtcat cgagaaggag
     1261 aacgggatcg aggtgtagag gttgttgaag gcggccgtgt gtcccagggg cgggtccacg
     1321 ttccagaaga tctggtgcga gcccgtcttc tcgagcccgc ttgtgcgctc cccgcagccg
     1381 aagtaccgtt ccccctcctc gcgcctcttg tacagccgcg gcgaagggtc gagcggctct
     1441 gtgaagacgt cggcttccgg ccgccgcgag aagcccatcc cgagctcctc ctcgtccgcc
     1501 gcgagcacgc gtcccccgac gtcggagaag aatatccgca aggggtcgag ggagacgtgg
     1561 gccgtcgccg ccgaagtgga gagcgtgagc tttccgtctg agctctccat cgagacctcc
     1621 accggctccc aatcctgctt cgctatggct tcggagtcgt accgcggcgt gctcccgttc
     1681 gggaaagccc cgacccggaa gatgtcctcg gcgagggcgg agacctccac gatcgtagat
     1741 ccggccttca agcgcacgct gcgcccgtcg tggtctacca gctctgctgc gcccagcggc
     1801 tcgtagttgg gctctatctg cggcgggttc tcgaagatct cggccaattt cgctcccttc
     1861 ttagcacgcc cccttgcagg gggcttgtca gcacgctccg gctggcgcct ccgcttgtca
     1921 gcatttcagc tttctctctg ccgttcgaat ctcgcctggt cgtgttttgt taggaggctt
     1981 tgcggcagta gctcagtcta gcgctgcgga gaggcgaagc gcaccaccgt agctgagaag
     2041 ccgcctgagg cgaagcccaa gg
//
LOCUS       DASTUV010000002         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1107371, whole genome shotgun sequence.
ACCESSION   DASTUV010000002 DASTUV010000000
VERSION     DASTUV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107371"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFT03_00010"
     CDS             <1..351
                     /locus_tag="VFT03_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846551.1"
                     /translation="EVVEAFLAAARDGDFDALLAVLDPDIVERVDTGSGTIVEVRGSE
                     DVARRAMAVSQLGLVARPALINGAPGWVSVLDGELYAIAALTVHNGRITKMDILLDPA
                     RLARLDLSADYFDS"
     gene            complement(753..1499)
                     /locus_tag="VFT03_00015"
     CDS             complement(753..1499)
                     /locus_tag="VFT03_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU4846552.1"
                     /translation="MIRAFVFDLDGTLVETEELKAISYARAATALRPDLNEGEVIAAF
                     GDLVGLSRQEVAVGLMRRFGLEEAARARMAEFGVDKPWQAYVQIRLGIYEEMLDDSKL
                     IQEYRYPHNIALLRSVRREGFPTALATQSHREQVRRVLDILGLADEFDVVVTRDDVEH
                     GKPDPEMHLLAARELGVQPGECLAVEDSPAGVQGALAAGTEVIAVTTNLTRQKFRDTD
                     LLDRSHVVDDPRALPGVVRRLIKDAREPPE"
     gene            1681..2163
                     /locus_tag="VFT03_00020"
     CDS             1681..2163
                     /locus_tag="VFT03_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU4846553.1"
                     /translation="MAGFVEGIFIAPEAGAEMKSVQAAAALEGCGLEGDRYCAGTGHW
                     SRFGRVCEATFIAAEDLHDIEQESGVGVKNGEHRRNVVTRGISLKALRRGEQFRVGEV
                     VFEYRGPRSVCRYIERLTEPGMTQALKGRGGICARVIENGTVRVGDEIEVLQHTEEHP
                     "
BASE COUNT          460 a          707 c          748 g          419 t
ORIGIN      
        1 gaggtcgttg aagccttcct cgccgccgcg cgcgatggtg acttcgacgc gctcctcgcc
       61 gtgctcgatc ccgacatcgt ggagcgcgtg gacacgggca gcgggaccat cgtcgaagtt
      121 cgcggctccg aggacgtcgc ccgcagggcg atggccgtct cacagctcgg cctcgtcgcg
      181 cggcccgcgc ttatcaacgg cgcacccggc tgggtctcgg tgctagacgg agaactctac
      241 gcgatcgccg ccctaacggt gcacaacggg aggatcacga agatggatat cctgcttgac
      301 cccgcacgcc ttgcccgcct cgacctcagc gcagactact tcgactcata gactatgagt
      361 cgggactctc ctatgagttg ggactcgaat cgagcaaaaa tgcccgagcc gagtagtagt
      421 agtagtagta gtagtagtga ggagcccttc actggagtcc acctcgggcg caaacgcaag
      481 aagaagcgcc aagtccttga gaatccgaag gtccacagga gattgacccc tggactctga
      541 caggacgcgt cttcgacgac aagcatgagc gcacgaccga cttgagccgc tgtgcggccg
      601 cccgcagcgc aacctctgcg ttggtctgtg tgcctctgaa accgaccacc gctcgcctct
      661 ggaatctatt ggccgtatgc agcaactagg aaccgagtca ggccttgtct ttctaggccg
      721 agactacgcc agtagtaacg gtacggatga gactactcgg gaggttccct tgcatccttt
      781 atcaggcggc gcacgacccc tggcagtgca cgagggtcgt ccacgacgtg gctgcgatcc
      841 agaagatcgg tgtcgcggaa cttctgacgg gtgagattgg tagtgacggc gatgacctcc
      901 gtcccagccg ctaacgcccc ctgcacccca gccggggagt cctcgaccgc caggcactcc
      961 cccggctgaa cgcccagctc ccgcgccgcc agcaggtgca tctccgggtc gggtttgccg
     1021 tgctcgacgt catcgcgggt aacgaccacg tcgaactcgt ccgccagccc caggatgtcg
     1081 agcacgcgcc ggacctgctc gcggtgggat tgcgtggcga gggccgtcgg gaaaccttcg
     1141 cgtcggacgc tccggagcag agctatgttg tgaggataac ggtactcctg gatcagtttc
     1201 gagtcgtcta gcatctcctc gtagatgcca agcctgatct gaacgtaggc ctgccacggc
     1261 ttgtctacgc cgaactccgc catacgagcc cgggccgctt cctcgagccc gaagcgccgc
     1321 atcaggccga cggctacctc ctggcgcgag aggcctacca ggtccccgaa ggccgcgatc
     1381 acttctccct cgttgaggtc cggacgcaat gcggtagcgg cgcgagcgta ggagatcgcc
     1441 ttcagctcct cggtttcgac cagggtaccg tcgaggtcga agacgaacgc gcggatcatg
     1501 ccggttccgc cccatttgct atccggacgc tctcgataac gtacgaacat ctcggcacgc
     1561 tcgggctcct ttcccaaacc gaagccgcaa aggttggcta atcggtgttt gcgatattac
     1621 tgtcctacca tactgaagaa cctctcgaga gagaggagca agaatcaaag gaaggctggt
     1681 atggcgggct tcgtggaagg catattcatt gcgccggaag ctggtgcaga gatgaagagc
     1741 gttcaggcgg ccgctgcact cgaaggttgc ggtctggagg gtgaccgcta ttgtgccggg
     1801 accgggcact ggagccgctt cggcagggtt tgcgaagcaa ccttcatcgc agccgaggac
     1861 ctacacgaca tcgagcaaga gagcggcgtg ggcgtgaaga acggcgagca ccggcgcaac
     1921 gtcgtcacca gagggataag cctgaaggcc ttgcgacgtg gcgagcagtt ccgagtcggg
     1981 gaagttgtct tcgagtaccg ggggccgcgc tccgtctgcc ggtacataga acggcttacc
     2041 gaaccgggta tgactcaagc cctcaagggt cgtggtggta tctgcgcgag ggtgatcgaa
     2101 aacggcacgg tccgggtggg cgacgagatt gaggtgctgc agcacactga ggagcatcca
     2161 tagaggcggg gtgaccgatg agctccagta ccgcccccgt tagtgagcga gcaggacgag
     2221 agcacggtgt cgttgcagta gaggtctagt aggtgacgga tggaggatcg agtgtgggct
     2281 cgacagcgcg cgctctacgt accgcgtaat gctgtcagaa gcgcctcgaa ctcg
//
LOCUS       DASTUV010000003         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_928294, whole genome shotgun sequence.
ACCESSION   DASTUV010000003 DASTUV010000000
VERSION     DASTUV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928294"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1224)
                     /locus_tag="VFT03_00025"
     CDS             complement(190..1224)
                     /locus_tag="VFT03_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4846554.1"
                     /translation="MRRRHVLAVLLAASVAVVAACGAQVRDSGGGGGGQGGGGGEGEN
                     APVQLAVVPKAIGFDFWEQVRLGAECAAKKASGQVDVQWDGVTSETDVNGQVNLLQNF
                     ITQGVDGLVYAATDAKVLSKVTQSAKQQGVTVVNIDSGTDPQPSDVPVFATDNVAAAE
                     QVPELMAKELGKEGGEVAFIPFQQGTLTNEQREEGFKKGLKQHPELELVAEQSSQSNY
                     NTALQVTEDMLTANPNLDAIFAANEPGVLGAAEAVRQAGKAGDIVIIGWDASSEEVQG
                     VEDGVISALVVQNPFRMGYDGTNAAVKIIREGAKVESEDTGSIIVTKDNLQDPKVQSV
                     LNPSCENPPV"
     gene            complement(1391..2386)
                     /locus_tag="VFT03_00030"
     CDS             complement(1391..2386)
                     /locus_tag="VFT03_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4846555.1"
                     /translation="MTAATEERGGRGALRERLSEVVSQLAAAGALIVVFVFLSFASPV
                     FLSADNLFNIGSQTAVVAVIAIGMTLVIITAGIDLSVGSVSALSGVLGVMMMAQAGLP
                     VPVAILGGTLVGATCGLVNGLLVSVGGLNPFIATLGMLTVARGLTFIVTNAVAVFGTP
                     NSFRLLGQGVIGPIPIPILVIVVVAVIGYIVLSRTRLGRYAYAMGSNLEAARLSGIPI
                     RRYLTSVYVISGALAGFGGMIAASRVNSGQPNFGIGLELDVIAAAVIGGASLFGGQGT
                     VVGTLIGAFLIALIRNGAVLLDVNTFYQQVIIGVVIWLAVFWDQYRRRRLAAASE"
     gene            complement(2383..>3899)
                     /locus_tag="VFT03_00035"
     CDS             complement(2383..>3899)
                     /locus_tag="VFT03_00035"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU4846556.1"
                     /translation="LPETKPPETRAPVVRFRNVSKEFPGVLAVDSVDLEIMPGEVHVV
                     AGENGAGKSTLMKLLSQVERPSGGEIEISDEAVEFHGPRHAQSLGVAMVYQEFALASH
                     LSIAENLYMGREPGRFGFVNRRAESQEARGLLERVGLHTDPDRTVSGLTVAEMQRVEI
                     AKALAIDAKVVIMDEPTATLAEKEIEGLFEVIRDLQSHGIAILYISHRLDEIFRIADR
                     VTVMRDGKIVDTVPIDDLDEDKLVRLMVGRDIENLYPKTEVEIGDVVLRAEGITRGHV
                     LKDCSFEVRAGEILGFAGLVGAGRTELARAVFGADRIDSGTIELDGRKLHIRGPQNAI
                     DAGIGYLTEDRKGEGLALQLGVDQNITLAHLPTRFGFINLAEERRIARRRRDELNIRT
                     PSIRRRVQVLSGGNQQKVVVARWLEARARVLFFDEPARGIDVGAKAEMFGLIEDLAKE
                     GRATVMISSYLPELLNMCDRILVMHEGEVAGVIPREEFSEERVMALAAGMEEIA"
BASE COUNT          646 a         1308 c         1225 g          720 t
ORIGIN      
        1 aaggcgacga gcggctgcgc gtcgtcgaag acccgcgccc catgggattc gacgccgacg
       61 gctccctgct ctgacgggcg tcctcgtgga ccacgaggag gcaggccgcc agagtagcgg
      121 ttcacgtgcc ggacagcgga agcggggaca cgtgatgcgc ccccgcttct tcgttctgca
      181 gcgcccggcc tagacgggtg ggttctcgca gctcgggttg aggacggact gtaccttcgg
      241 gtcttgcagg ttatctttgg tgacgatgat ggagcccgtg tcctcgctct ccaccttggc
      301 gccttcgcgg atgatcttga ccgctgcgtt ggtgccgtcg tagcccatcc tgaaggggtt
      361 ctggacgacg agggcgctga tcacaccgtc ttcgacgccc tggacctcct ccgaggatgc
      421 gtcccagccg atgatgacta tgtcgccggc cttgccagcc tggcgcacgg cctcggcggc
      481 gccgagcaca ccgggctcgt tggcggcgaa gatcgcgtcg aggttggggt tggcggtcag
      541 catgtcctcg gtgacctgga gagcagtgtt gtagttgctc tggctcgact gctcggcgac
      601 gagctcgagc tcggggtgct gcttcaggcc cttcttgaag ccctcctcgc gttgctcgtt
      661 ggtgagggtc ccctgctgga aggggataaa ggcgacctct ccaccctcct tgcccagctc
      721 cttggccatc agctccggga cctgctcggc ggcggcgacg ttgtccgtag cgaagacggg
      781 gacgtcggag ggctgagggt cggtgcccga gtcgatgttc accacggtca cgccctgctg
      841 cttggccgac tgggtcacct tggagaggac cttggcgtcg gttgcggcgt agacgaggcc
      901 gtcaacgccc tgggttatga agttctgcag caggttcacc tggccgttga cgtcggtctc
      961 gctggtgacg ccgtcccact ggacgtctac ctggcccgag gccttcttgg cggcgcactc
     1021 ggcgccgagg cgtacctgct cccagaagtc gaagcctatc gccttcggca ccaccgcgag
     1081 ctggaccggg gcgttctctc cctcgccacc gccgcctccc tggcccccgc ctccgccgcc
     1141 ggagtccctc acctgcgcgc cgcacgccgc gaccacggcg acggaggcgg ccaggagcac
     1201 cgccaggacg tgtcttctac gcattcgttc ctccttttcc cgttgagacg ctgcatgttg
     1261 tagagaggtt gtgtgttgtg gagatgttgc atgcagtaga gacgttgcat gcaacatctc
     1321 tacgcgtaca acctctctat ccatacaacg tctctacccg tgcaacttct ctatccatac
     1381 gacctctcta tcactccgat gcggcggcga gccggcggcg gcggtactgg tcccagaaga
     1441 ccgcgagcca gatcaccacg ccgataatca cctgctggta gaaggtgttt acgtcaagga
     1501 ggaccgctcc gttacggatc agggctatga ggaaggcccc aatcagggtc cccacgaccg
     1561 tcccctgccc cccgaagagg ctcgccccgc cgatgacggc cgcggcgatc acgtcgagct
     1621 cgagccctat gccgaagttc ggctgccccg agttgaccct cgaggcggct atcatgccgc
     1681 cgaagcccgc aagagctccg gagatcacgt acacgctcgt caggtagcgc cttatcggta
     1741 tccccgagag ccgggcggcc tccaggttcg agcccatcgc gtaggcgtag cgtccgaggc
     1801 gggtgcggga gagcacgatg tatccgatga ccgccactac cacgatcacg agaatcggta
     1861 ttggtatcgg cccgatgacc ccctgcccca ggagtctgaa ggagttcggc gtgccgaaga
     1921 cggcgacggc gttggtgacg ataaaagtca aacccctggc gacggtgagc atgccaaggg
     1981 tggctatgaa cgggttgagg ccgccgaccg atacgaggag cccgttgacg agcccacatg
     2041 tggctccgac cagggtgccg ccgaggatcg ccacagggac gggtaggccg gcctgggcca
     2101 tcatcatcac gccgagcacc cccgagagcg cagacaccga gccaaccgag aggtctatcc
     2161 ctgcggtgat gatcaccagc gtcatcccga tggctatgac ggccacgacg gcggtctggg
     2221 agccgatgtt gaagaggttg tcggcggaga ggaagacggg ggaggcgaat gagaggaaga
     2281 cgaagacgac gatgagtgcg cctgcggcgg cgagctgaga gacgacctcc gagagcctct
     2341 cgcgcagagc gcctctaccg ccgcgttctt cggtcgctgc tgtcatgcga tctcctccat
     2401 tccggcggcg agcgccatca cccgctcctc tgagaactct tcgcggggta tcacccctgc
     2461 tacttcaccc tcgtgcatca ccaggatgcg gtcgcacatg ttcaacagct ccggcaggta
     2521 ggacgagatc atgaccgtcg cccgcccctc cttcgccaga tcttcgatga ggccgaacat
     2581 ctcggccttc gcccccacgt ctatgccgcg ggcgggctcg tcgaaaaaca gcaccctcgc
     2641 cctggcctcg agccacctgg cgacgaccac cttctgctgg ttgccaccgg agagcacctg
     2701 caccctgcgc cggatcgagg gcgtccttat gttcaactcg tcccggcggc gccgggcgat
     2761 ccggcgctcc tccgccaggt ttatgaaccc gaagcgggta ggcagatggg ccagcgtgat
     2821 gttctggtcc accccgagct ggagcgccag gccctcgccc ttgcggtcct cggtaaggta
     2881 gccgatgcca gcatctatcg cgttctgagg ccctctgata tgcagctttc gtccatcgag
     2941 ctcgatggtt cccgaatcta tgcggtcggc cccgaagact gcccgggcca gttccgtgcg
     3001 gcctgccccg acgaggccgg cgaagcccag gatctcgccg gctcgaacct caaaggagca
     3061 atccttgagg acgtggccgc gggtgatccc ttctgcgcga agcaccacgt ccccgatctc
     3121 cacctccgtc ttgggataga gattctcgat gtcgcgtccc accatcagac gcaccagctt
     3181 gtcttcgtcc agatcgtcga tcggcacggt atcgacgatc ttgccgtcgc gcatgaccgt
     3241 cacccggtcg gcgatgcgga agatctcatc cagcctgtgc gagatgtaca ggatcgctat
     3301 gccgtgggac tggaggtccc ggatcacctc gaagaggccc tcgatctcct tctccgcgag
     3361 cgtggccgtc ggctcgtcca tgatgacgac cttcgcgtct atggcgagcg ccttggcgat
     3421 ctcgacccgc tgcatctcgg cgaccgtgag gcccgagacg gtccggtccg ggtcggtatg
     3481 caggcccacg cgttcgagca acccgcgcgc ctcctggctc tcggctctcc ggttcacgaa
     3541 cccgaaacgc ccgggctcgc gtcccatata gaggttctcg gctatcgaga ggtgcgaagc
     3601 cagggcgaac tcctggtaga ccatcgctac gcccagggac tgagcgtggc gcggaccgtg
     3661 aaactcgacg gcttcgtcgg agatctcgat ctcccccccg ctcggccgct cgacctgcga
     3721 gaggagcttc atcagcgtgg acttgccagc cccgttctca cccgccacca cgtggacctc
     3781 gcctggcatt atctccaggt ccacactatc gaccgcgaga acaccgggga actctttgga
     3841 gacgttccgg aaccttacga ccggggctct ggtctcgggt ggcttcgtct caggtaacg
//
LOCUS       DASTUV010000004         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1750874, whole genome shotgun sequence.
ACCESSION   DASTUV010000004 DASTUV010000000
VERSION     DASTUV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750874"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFT03_00040"
     CDS             complement(<1..345)
                     /locus_tag="VFT03_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846557.1"
                     /translation="MCDLHQRATVKVLVAASLVFFSLLIASCSKDTGSQGTGPTKKSD
                     GFKEGTLVSSSRGAEKTAEAPPPEATLGPEADSPAVLLRLEGDPGTTFSGICVAGSKQ
                     SVLSGRIPKSYAF"
     gene            complement(406..1104)
                     /locus_tag="VFT03_00045"
     CDS             complement(406..1104)
                     /locus_tag="VFT03_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4846558.1"
                     /translation="MLRAEPEGRSVLQRSEKLAALQREIVSCRRCPRLVEWREKVARE
                     KRAAYSEETYWGRPVPGFGDPEAKVVILGLAPAAHGANRTGRYFTGDRSGDFLFAALH
                     RAGFADKPVSRTIEDGARLSGLWISAAVRCAPPQNKPTTAERDACLPYAVRELELLEA
                     EVVVCLGAFAWDSALRIHGIRPKPRFGHGAEAPAGDATLLGCYHPSQQNTFTGVLKPE
                     MLDAVLLRARELTG"
     gene            complement(1378..2586)
                     /locus_tag="VFT03_00050"
     CDS             complement(1378..2586)
                     /locus_tag="VFT03_00050"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU4846559.1"
                     /translation="MDLELMRELSVKTDSKIVLLILDGLGGLPLDPAGPTELEAAEAP
                     NLDALAARSDLGLSVPVAAGVSPGSGPGHLALFGYDPLRYVVGRGVLSALGVGFDLGE
                     NDLAARLNFATLDEKGRISDRRAGRIPSEKGAELVEMLNENLDLEGVEVYVIPEKEYR
                     AVAIFRGEGLSDALADSDPQRVGLEPLPVKPTDGSPEAERSAALANAFVEQANEILRD
                     QHPANTLLMRGFGMHPGLPSFEEIYDLDAAAIASYPMYKGLARLAGMELLKEGEGIAG
                     EFDTLRQNWDRHDFFFLHVKPTDAAGEDGDFERKAAVIEEVDAQVPGLLDLGPDALAV
                     TGDHATPAKLKSHSWHGVPFLLASPYTLPTAESFGERTCAGGSLGVFAAEEIMGLLMG
                     HALKLNRYGA"
     gene            2731..3102
                     /locus_tag="VFT03_00055"
     CDS             2731..3102
                     /locus_tag="VFT03_00055"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4846560.1"
                     /translation="MLKGLRTAIYHVGDLDRAKGWYGEVLGKEPYFDEPFYVGFDVGG
                     FELGLQPDEGGAPRSSTGVVAYWGVDDAEAALARLLELGATGRSGVHDVGEGILLASV
                     LDPFGNVFGIIENPHFSLEGA"
     gene            complement(3190..3666)
                     /locus_tag="VFT03_00060"
     CDS             complement(3190..3666)
                     /locus_tag="VFT03_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4846561.1"
                     /translation="MEERTVEVNYFPKKILLATDASKDAEKAALIASDIANSTGSELH
                     VLHVGNTKDFHIAPGAEQSFSPRTGSLGEIREKAEKTLGQAVEQVEEAGGTVAQAHLR
                     LGDPDDEILRFCDEQGGFGLIVMGSRGLTRIKRVVMGSVSESVVRHAHCPVLVARS"
     gene            complement(3939..>4232)
                     /locus_tag="VFT03_00065"
     CDS             complement(3939..>4232)
                     /locus_tag="VFT03_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU4846562.1"
                     /translation="LYDLEDINPEFEDADAAIIVGANDVVNPAANDPEQDSPISGMPI
                     LNVEAADRVIFIKRSLSPGFAGVDNPLFYDTEKTMMLFSDAKQGLQDVTSALK"
BASE COUNT          683 a         1361 c         1396 g          792 t
ORIGIN      
        1 gaaggcgtaa cttttgggta tccggccgct caggacgctc tgtttgctcc ctgcgacgca
       61 gatgccggag aaggtagtcc cggggtcccc ttcgagacgg agcaggacgg ccggcgaatc
      121 cgcctccggg cctagagttg cttcgggggg tggggcttcc gcggtcttct ccgcccctct
      181 ggaggaagag accagggtgc cttccttgaa gccgtcggac ttcttggttg ggcctgtgcc
      241 ctgtgatccc gtatccttgc tacaggacgc gatcagcaaa gagaaaaaga cgagcgaagc
      301 cgccaccaga accttcacgg tcgcacgttg gtgaagatcg cacacctacc ggattttatc
      361 cgaagggaac gctaaattcc ggagatcccg ggccgcgcgg aacactcacc cggtcagctc
      421 ccgcgcccgc aaaagcaccg catcgagcat ctcgggcttg aggacgccgg tgaaggtgtt
      481 ctgctgggaa gggtggtagc agccgaggag ggtcgcgtcg cccgccggcg cctcggcgcc
      541 atggccgaac ctgggcttgg gccgtattcc atgtatgcgg agggcggagt cccaggcgaa
      601 ggcgcccagg cagacgacca cctcggcctc gagcagctcg agttcgcgca cggcgtacgg
      661 gaggcaggcg tcgcgctcgg cagtcgttgg tttgttctgc ggcggggcgc agcggacggc
      721 ggcggagatc cagagtcccg agaggcgcgc gccgtcttct atggtgcgtg agaccggctt
      781 gtccgcgaag cccgcgcggt gcagcgcggc gaagaggaag tcgcccgaac ggtcgccggt
      841 gaagtacctg cccgtcctgt tcgcgccgtg ggcggcgggc gcgagaccga ggatcacgac
      901 cttcgcctcc gggtctccga agccggggac gggacggccc cagtaagtct cctccgagta
      961 ggccgctctc ttctcgcggg cgaccttctc gcgccactcc acgagccgcg ggcagcgccg
     1021 gcaggagacg atctcgcgtt gcagcgcagc gagtttctcg ctgcgctgca acacgcttcg
     1081 cccttcgggc tcagctctca gcacgcctcc cttcttagca cgccgcctcc catcagtcgg
     1141 cggcttgtca gcacgctcgg gcctttttaa aggccctcgc ttgtcagcat ttcagctttc
     1201 tctctgccgt tcggcacttg cccagtcacg cctggagaga cacttttggg tataagcttg
     1261 ggctggcgca gggaagtacc atgtcgcgtc ctccttgctt ttagctgaga aaccgcctgg
     1321 cggagccaaa ggcggcgtgc tgacaagcgg agccctcgaa gagggcgtag cgtgctctca
     1381 cgcgccgtag cggtttagct tgagggcgtg gcccatgagg aggcccatga tctcctccgc
     1441 tgcgaagacg ccgagcgagc cgcccgcgca cgtgcgctcc ccgaagctct ccgcggtcgg
     1501 cagagtatat ggagatgcga gcaggaacgg cacaccgtgc caggagtggc tcttcagctt
     1561 cgcgggggtc gcgtggtcgc cggtgacggc cagcgcgtcg gggccgaggt cgagcaggcc
     1621 aggcacctgg gcgtcgacct cctcgataac cgcggctttg cgctcgaagt cgccgtcctc
     1681 gccggctgcg tcggtcggct ttacgtggag gaagaagaag tcgtggcggt cccagttctg
     1741 ccgcaaggta tcgaactcgc cggctatgcc ctcgccctcc ttcaggagct ccatccctgc
     1801 gaggcgggcg aggcccttgt acatcgggta gctcgctatg gcggcggcgt cgagatcata
     1861 gatctcctcg aaggagggga gtcccgggtg catcccgaag ccccgcatga ggagggtatt
     1921 ggccgggtgc tgatcacgca ggatctcgtt cgcctgttcg acgaaggcgt tcgccagagc
     1981 ggcgctccgc tcggcctccg gggagccatc cgtcggtttg acggggagcg gttcgagtcc
     2041 gacccgctgg ggatccgagt cagccagcgc gtcagagagg ccctccccgc ggaagatagc
     2101 gacggccctg tattccttct cggggatcac gtatacctcg acgccttcga gatctaagtt
     2161 ctcgttgagc atctcgacga gctccgcgcc cttctccgat gggattcggc ccgccctgcg
     2221 gtcggagatc ctgcctttct cgtcgagggt cgcgaagttg agccgcgcgg ccaggtcgtt
     2281 ctcgcccagg tcgaagccaa cgccaagggc gctcaggacg ccgcgcccga ctacgtagcg
     2341 caaaggatcg tagccgaaga gggcgaggtg gcccggtccg ctgccggggc tgacacccgc
     2401 ggccacggga accgagagcc caaggtccga ccgggcggcc agagcgtcga ggttgggggc
     2461 ctcggccgcc tccagctcgg tcgggccggc ggggtcaagg gggagcccgc cgaggccgtc
     2521 caggatgaga agcacgatct tcgagtccgt cttgacagac agctcgcgca tgagttcaag
     2581 gtccaaaacg ctcctcctat ctagcggttc cggcagtcta tcagacggtt tcccgccagc
     2641 gccccgctcg aaactgattg cgcctttgga ggcggcgacg gcgcccgtat actcatcccg
     2701 tcacggatct tctcgggaga ggagggcagt atgctcaagg gcttgaggac tgctatctac
     2761 cacgttggcg acctcgatcg ggcgaaggga tggtacgggg aggtgctggg gaaagagccg
     2821 tacttcgacg agccgttcta tgtcgggttc gatgtcggcg gcttcgagct cgggttgcag
     2881 cctgacgaag gcggcgctcc ccggtcttcg accggcgtgg tcgcctactg gggcgtggac
     2941 gacgcggagg ccgcgctcgc ccgcctcctc gagctcggcg ccacagggcg ctccggcgtc
     3001 catgacgtgg gggagggcat cctgctcgcc tccgttctcg acccgttcgg caacgtcttc
     3061 ggcatcatcg agaacccgca cttctcgctc gagggcgcgt agcggccaga cgacgccatt
     3121 cgatggagac aggccctacg acctgcctcg actcccgcga tagagccccc gaatagaggg
     3181 cgagatcttt taactgcgtg ctaccaggac ggggcagtgc gcgtgcctga cgacgctctc
     3241 ggagacgctc cccatgacga cgcgctttat ccgggtcaac ccgcggcttc ccatgacgat
     3301 cagaccgaac ccgccctgct cgtcacagaa cctgagtatc tcgtcgtccg ggtcgcccaa
     3361 cctcaggtgc gcctgcgcga cggttccccc ggcttcctcc acctgctcca cagcctgccc
     3421 gagcgtcttc tcggccttct ccctgatctc acccaatgag ccagtacgag gggagaagct
     3481 ctgctccgcg ccaggggcga tatggaagtc cttggtgttc ccaacgtgga gaacgtgcag
     3541 ctccgagccg gtgctgttcg ctatgtcgct cgctatcaga gccgccttct ccgcgtcttt
     3601 tgaagcgtcg gtcgccagta ggatcttctt cgggaaataa ttcacctcaa ccgtcctttc
     3661 ctccaccaac cctggcatca ttctaccagt agcgccattt aagcacgctc cggcttcgcc
     3721 gtcgcttgtc agcacgcatc gggcctttag taaaggccct cgcttctcag catttcagct
     3781 tcttctgccg ttgggctcat gcccggtcac gctttccgag acggccttac agcggtagct
     3841 ctgactagcg ctgcggagcg atgaagtgcg ccatcctggc tgacaagcct gcgaagcagg
     3901 cgtgctgaca agctgcctgg cgaagccggg cagcgtgctt acttcaaagc ggaggttacg
     3961 tcctggaggc cctgcttggc gtcggagaag agcatcatgg tcttctcggt gtcgtagaag
     4021 agggggttat ccacgccggc gaagccaggg ctcagggagc gttttatgaa gatgaccctg
     4081 tctgcggcct cgacgttcag gatgggcatt ccggagattg ggctatcctg ttccgggtcg
     4141 ttggccgcgg ggttgacgac gtcgtttgcg ccgacgatga tcgcggcgtc cgcgtcctcg
     4201 aactcggggt tgatgtcctc gaggtcgtag ag
//
LOCUS       DASTUV010000005         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_571246, whole genome shotgun sequence.
ACCESSION   DASTUV010000005 DASTUV010000000
VERSION     DASTUV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571246"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFT03_00070"
     CDS             <1..580
                     /locus_tag="VFT03_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HEU4846563.1"
                     /translation="GIRLATDQNLIITGVPEDRLDDLLAEPLLARYSPNPGAFERGVV
                     ACTGSEFCRFGIVETKVRAVEWAREMDERVGDPGQEAVRMHFSGCSASCAQPQIGDIG
                     FRGETAKTRDAIVEGVDIGLGGSLGKDAAFIDWVEGAKPAGEVPDALVAVFEQFEKER
                     REGERFHEWARRKKNAELREALRKPVESEVGS"
     gene            580..1779
                     /locus_tag="VFT03_00075"
     CDS             580..1779
                     /locus_tag="VFT03_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEU4846564.1"
                     /translation="MAGFDIREMMNDIDEAPGKVWFWDLEKAVIDADRCVQCGVCVAA
                     CPTDSIGIGEDDLPQLVKMCTGCSLCWDFCPRGGLQYESTWKITGSSNGKSIEGMGRV
                     EESYTARVRQRIDGVQDGGFVSALLISLLEAGEIDGALLARESATERWKGEAFLATTP
                     EEVRACAGSFYNQTLALGHVDFEDYDLPPNPRIAVVGTPCEIEGIKAMQARPWTWGSS
                     KVEAITLTIALLCTKSFNYEKLMLEEIRDKRNVDLDNVGRVDVIRGKMVVQDLEGVTI
                     FEEPIRDFHGAALKGCDECADFMGHAADISVGSVGSADGYSSVLLRSEEGLAAFDSVR
                     DRLELRPLDKPQALDKLDGLDKRVAFGSLKRAFDPDAPLFIDYEEHLNDYAGTDRAPV
                     EHEAVRY"
     gene            1780..2121
                     /locus_tag="VFT03_00080"
     CDS             1780..2121
                     /locus_tag="VFT03_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846565.1"
                     /translation="MKGDVKRLSVPDEPVEIKARTRTLLEDSPEEGSRVIADAAFVSD
                     LLWEEWGADLEGAGMGYDRFLEISRSYAGEFRFWIVGERPWDHCAAGLAGRLLRRLPA
                     RQATNLAEVNG"
     gene            2118..>2327
                     /gene="sat"
                     /locus_tag="VFT03_00085"
     CDS             2118..>2327
                     /gene="sat"
                     /locus_tag="VFT03_00085"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEU4846566.1"
                     /translation="MNVSTEFTTIAPHGGELVDRMAPEDQRDELLSRAEGFASVTLGP
                     RALSDLEMISTGVFSPLTGFMVSEDY"
BASE COUNT          442 a          673 c          818 g          394 t
ORIGIN      
        1 cggcatccgg ctcgctaccg accagaacct cattatcacg ggcgtccccg aggaccgcct
       61 ggatgatctt ctcgccgagc cgcttctcgc gcggtattcc ccgaaccctg gggcgttcga
      121 gcgcggggtt gtggcgtgca cggggagcga gttctgccgc ttcggcatcg tcgagaccaa
      181 ggtcagggcc gtagagtggg cccgcgagat ggacgagagg gttggggacc ccggccagga
      241 ggccgtgagg atgcacttct cgggctgctc ggcctcctgc gcgcagcccc agatcggaga
      301 cataggcttc cgcggcgaga ccgcgaagac gcgggacgcc atcgtggagg gcgtagacat
      361 agggcttggc ggctctcttg gcaaggatgc agcgttcatc gactgggtcg agggcgcgaa
      421 gccggcaggg gaggtccctg acgccctcgt tgccgtcttc gagcagttcg aaaaggagcg
      481 gcgagagggc gagcgcttcc atgagtgggc caggcgcaag aagaacgccg agctgcggga
      541 ggcgctgcgc aagccggtcg agagcgaggt tggttcatag tggccggctt cgatatcagg
      601 gagatgatga acgacatcga tgaggctccg ggcaaggtct ggttctggga cctggagaag
      661 gcggtgatcg acgccgaccg ctgcgtgcaa tgcggcgtgt gcgtcgcggc ctgccctacc
      721 gactccatcg gcatcggcga ggatgaccta ccccagctcg tcaagatgtg cacaggatgc
      781 tcgctgtgct gggacttctg cccccgcggc ggcctgcagt acgagtcgac ctggaagatc
      841 actggcagct ctaatggtaa gagcatcgag ggcatggggc gcgtcgagga gagctacacg
      901 gcccgtgtca ggcagaggat agacggcgtg caagacggtg gattcgtcag cgcgcttctg
      961 atctcgctgc tcgaggccgg cgagatcgac ggcgccctgc tggcgcgcga gagcgcgacc
     1021 gaacgctgga agggcgaggc tttcctggcg acgacgcccg aggaggtgcg ggcatgcgcg
     1081 gggagcttct ataaccagac gctcgcgctc gggcacgtgg acttcgaaga ctacgacctg
     1141 ccgccgaacc ccaggatcgc ggtcgtgggg acgccgtgtg agatcgaggg catcaaggcg
     1201 atgcaggcca ggccatggac gtggggcagc tcgaaggtcg aggccataac gctcaccata
     1261 gccctgctct gtaccaagag cttcaactac gagaagttga tgctcgagga gatcagggac
     1321 aagaggaacg tcgacctgga taacgtgggg cgggtggacg tcatacgagg gaagatggtc
     1381 gtacaggatc tcgaaggcgt gacgatcttc gaggagccta taagggactt ccacggtgcg
     1441 gccctcaagg gttgcgacga gtgtgcggac ttcatggggc acgccgccga catctccgta
     1501 gggagcgtgg ggagcgcgga cggttactcc agcgtgctcc tccggagcga agaaggcctg
     1561 gccgccttcg acagcgtcag ggatagattg gagcttcggc cccttgacaa gccgcaggct
     1621 ttggataagc tagacggttt ggacaagagg gtggccttcg gtagtctcaa gcgggccttc
     1681 gacccagacg ccccgctgtt tatagactac gaggaacacc tgaacgatta cgcagggacg
     1741 gaccgcgccc cggtggaaca cgaagcggtg aggtactagt tgaaaggcga tgtaaagagg
     1801 ctctctgtgc cggatgagcc cgttgagata aaggccagga cgcgtaccct actcgaggat
     1861 tcgcccgagg agggtagtcg cgttatcgcc gacgccgcct ttgtctctga tctcctctgg
     1921 gaggaatggg gggccgacct cgaaggagcc ggcatgggct acgaccggtt cctcgagata
     1981 tcgcgctctt acgcgggcga attcaggttc tggatcgtcg gcgaacgtcc atgggaccac
     2041 tgcgcagccg gcctcgccgg ccgcttgttg cgtagactcc ccgcccggca ggccactaat
     2101 ctcgcggagg taaacggatg aacgttagca ccgaattcac tacaatcgcc ccgcacggcg
     2161 gcgagctcgt cgaccgcatg gcccctgagg accagcgtga cgagctactc agtagggccg
     2221 agggcttcgc cagcgtaacc ctgggtccgc gggctctctc tgacctggag atgatctcca
     2281 ccggcgtctt ctctcccctc acgggcttca tggtcagcga ggactac
//
LOCUS       DASTUV010000006         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_34738, whole genome shotgun sequence.
ACCESSION   DASTUV010000006 DASTUV010000000
VERSION     DASTUV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34738"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFT03_00090"
     CDS             <1..578
                     /locus_tag="VFT03_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846567.1"
                     /translation="DLPLNVVALVPATENLPGGDAFKPGDVLKMHSGKSVEIVTTDAE
                     GRLILADALSYARRYEPSAVVDCATLTGACHVALGDYASGLMGNDEDLISEIQTAGEA
                     TGERSWPLPLFDEYTEQIRGDVADIKNSGGRYGGALTAGAFLKEFADYPWAHLDIAGT
                     AYGKKGNAYTPKGATGAPARLLVEFLRGRTR"
     gene            575..1213
                     /locus_tag="VFT03_00095"
     CDS             575..1213
                     /locus_tag="VFT03_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4846568.1"
                     /translation="MILEMLTVGPFQENCYIIGDEQTGTGALVDPGDEAARIALAVEQ
                     TGLDIGQIIITHAHIDHVGAVAALADEYACPVLMHAEAEPMLQQLPTQAMMMGLRFGK
                     LPTVDHHIEDEEVLEVGGLRLRSLYTPGHAPGHLAFYLEDQGLVLSGDALFAGSVGRT
                     DLFGGSMDLLMRSINERLLTLPDETRVLSGHGPETTIGEERARNPFLGGELL"
     gene            1210..1734
                     /locus_tag="VFT03_00100"
     CDS             1210..1734
                     /locus_tag="VFT03_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU4846569.1"
                     /translation="MSESVHRHRESAPEHVRLAVLTISDTRTPENDTGGDTIEELMRD
                     AGHEIVARRIVKDDAARIRTEIVNLLARPDADAVITTGGTGISARDTTYEVVDRMIEK
                     KLDGFGEIFRALSYEEIGAAAILSRAVAGTVGTKLVACLPGSRNAVRLAVEKLLVPEI
                     AHVIFELRKHQEGS"
     gene            1734..1991
                     /locus_tag="VFT03_00105"
     CDS             1734..1991
                     /locus_tag="VFT03_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846570.1"
                     /translation="MEREPLTPETETLWRRLWDIWQENDEEDVILDSLILGELEEEIP
                     DLEGRTKTALAYLQRARYIQYRSGVGEEGLEPILFDVYEPR"
     gene            2000..2437
                     /locus_tag="VFT03_00110"
     CDS             2000..2437
                     /locus_tag="VFT03_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4846571.1"
                     /translation="MAWILLPVALAAGVVLPVQFGVNSHLRTFVGGPLAAAAISFVVG
                     AVALGVAALVVQRSLPGSVVSAPWWAWTGGLLGALFVLASIILTPRLGAATTIGLILT
                     GQVLASIVIDHFGLIRVPMHEASFPRIIGALLIVAGVVMVQRF"
     gene            2451..3032
                     /locus_tag="VFT03_00115"
     CDS             2451..3032
                     /locus_tag="VFT03_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU4846572.1"
                     /translation="MRSVCVFCGSKPGNDPAYAAGARRLGRTLAEEGITLVYGGGHVG
                     LMGVVADTILDAGGKVTGVIPMALVEREIAHPGVTDLRVVGSMHERKALMSELSEGFI
                     ALPGGTGTLEEFFEVLTWAQLGEHEKPCGLLNLASYYDPLVALFDHMVAKGFLSEEHR
                     ATVLVETEPEPLLDAFAHYSPPNTVKWIDKEET"
     gene            complement(3111..3815)
                     /locus_tag="VFT03_00120"
     CDS             complement(3111..3815)
                     /locus_tag="VFT03_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEU4846573.1"
                     /translation="MLASLLIIVLGITLFFRTDPFQRASETVSVERVAVPMIGGAPEA
                     VAEQGQAAEEQTSSRKDAESGEEDTQREQAEAEKPVASVPATNDLWMTIPKLGLYDNY
                     VSNSNAHAALDAGAAKRVESGFPWESHANTYIAAHRLGWPGTASDHQFYNLPLLDYGD
                     KIYLYDANGTTYTYEVTEIFEVLPSETWVASSDPGRDMVSLQTCIETYGDYWTMGPNW
                     YVRYVVRADRVSVVPG"
     gene            4019..4171
                     /locus_tag="VFT03_00125"
     CDS             4019..4171
                     /locus_tag="VFT03_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846574.1"
                     /translation="MESLERHEELVRGCSLCFAGGGNTLVVDVPEDVRVLRSWQGAGW
                     RGQGAI"
     gene            4484..>4915
                     /locus_tag="VFT03_00130"
     CDS             4484..>4915
                     /locus_tag="VFT03_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4846575.1"
                     /translation="MSQTQSRTATLVARDLKKTYNGFEAVKGVDFEVHKGECFGFLGP
                     NGAGKTTTMKMIYGAVIPSGGELEVAGLDVTRCEREIKRRIGVVPQENNLDDDLKVEE
                     NLLVYGRYYDLPRKVALQRAEELLDFVELTEKAESQVEQLSG"
BASE COUNT          913 a         1526 c         1634 g          842 t
ORIGIN      
        1 tggacctccc gctcaacgta gttgcgctcg tgccggcgac ggagaacctg cctggtgggg
       61 acgcgttcaa gccaggcgac gtcctcaaga tgcactcggg caagtcggtc gagatcgtca
      121 cgaccgacgc cgaggggcgt ctgatcctgg cggatgccct ctcctacgcc cgacgctacg
      181 aaccgtcggc ggtcgtggac tgcgcaaccc tgaccggtgc gtgccacgtc gccctcggcg
      241 actatgcttc gggcctcatg ggcaacgacg aggacctgat ctcggagatc cagaccgccg
      301 gggaagcgac cggagagcgg tcctggcccc ttcccctctt cgacgagtac accgagcaga
      361 tacgggggga cgtcgccgac ataaagaact ccggcggccg gtacggcggc gccctgacag
      421 ccggcgcctt cctcaaggag ttcgccgact acccctgggc gcacctcgac atagcaggca
      481 ccgcctacgg caagaagggc aatgcgtaca cgccgaaggg cgccaccggc gccccggccc
      541 ggcttctggt cgagttcttg aggggacgga cgcgatgatc ctcgagatgc tcaccgtcgg
      601 accgttccag gagaactgct acataatcgg ggacgagcaa accggcaccg gcgccctcgt
      661 tgaccctggc gacgaggccg cccgcatcgc gctcgcagta gagcagacgg ggctcgatat
      721 aggccagatc atcatcaccc acgcccacat cgaccacgta ggggctgtcg ccgctctggc
      781 cgacgagtac gcgtgccccg tcctcatgca cgccgaggcc gagccgatgc tccagcagct
      841 ccccacacag gcgatgatga tgggcctcag gttcggcaag ttgcccaccg tagaccacca
      901 catcgaggac gaagaggtcc tcgaggtcgg cggcctgagg ctgaggtcgc tgtacacgcc
      961 agggcacgcg cctgggcacc tggcgttcta cctcgaggac caggggctcg tgctctctgg
     1021 cgacgcgcta ttcgcaggga gcgtaggaag gacagatctt ttcggcggca gcatggacct
     1081 cctgatgcgg agcattaacg agaggctcct gacgctgccg gacgagacga gggtgctctc
     1141 cggccacggt cccgagacca ctatcggcga ggagcgggcc cgcaacccct tcctgggcgg
     1201 agaactgttg tgagcgagag tgtccacagg caccgggagt cggcgcctga acacgtccgg
     1261 ctggccgttc tcaccataag cgacacccgc accccggaga acgataccgg cggggacacc
     1321 atagaggagc tcatgcgcga cgccggccac gagatcgtgg cgaggcgaat cgtaaaggac
     1381 gatgcggcgc gcatccggac ggagatagtg aacctgctcg cccgtcccga cgcagacgct
     1441 gtcattacaa ccggcgggac agggatctcg gcccgcgaca cgacttacga ggttgtggac
     1501 cgcatgatcg agaagaagct cgacgggttc ggcgagatct tccgcgcgct ctcctacgag
     1561 gagatcgggg cagccgcaat cctcagccga gcggtcgcgg gcacggtcgg caccaagctc
     1621 gtcgcctgcc tccccggatc gcgcaacgcc gtccgtctcg cggtcgagaa actcctcgta
     1681 ccggagatcg ctcacgtcat cttcgaactc agaaagcatc aggagggcag ctaatggaac
     1741 gcgaaccgct cacccccgag acagagacgc tctggcgcag gctctgggat atctggcagg
     1801 agaacgatga agaagacgtg atactcgact ccttgatact cggggagctg gaggaggaga
     1861 tccccgatct cgaagggagg acgaagacgg ccctcgcgta cctccagcgc gcccgctata
     1921 tccagtaccg ctcgggcgtc ggggaggagg gcctcgagcc catcctcttc gacgtctacg
     1981 aaccgcggta gtaatgagga tggcctggat cctgctgccg gtcgcgctcg cggccggagt
     2041 cgtcttgccc gtgcagttcg gcgtcaacag ccacttgagg accttcgtcg gcgggcccct
     2101 ggccgccgcc gcgatctcct tcgtagtcgg cgcggtcgcg ctcggcgtcg ccgcgctggt
     2161 ggtgcagagg tcgttgcctg gctcggtcgt cagcgcgccg tggtgggcgt ggacgggagg
     2221 tctgctcggc gccttgttcg tcctcgcctc gataatcctc accccgcgcc tcggcgccgc
     2281 aactactatc gggctgatcc tcaccggaca ggttctcgcc tcgatagtga tcgaccactt
     2341 cggcctgatc cgggtgccta tgcacgaggc cagctttccg cgcatcatcg gagcactcct
     2401 tatagtcgcc ggggtagtga tggtgcagag attttgagga gaagcaggac ttgaggagcg
     2461 tctgcgtctt ctgcggctcg aagccgggca acgacccggc ctacgcagca ggagcccgcc
     2521 ggctcggcag gacgctggcc gaggagggta ttacgctggt ctatggcggc gggcacgttg
     2581 ggctcatggg cgtagtagcc gacactatcc tcgatgccgg cggcaaggtt acgggagtca
     2641 tcccgatggc gctcgtggag cgtgagatcg cgcaccccgg cgtcaccgac ctgcgcgtgg
     2701 tcggctcgat gcacgagcga aaagccttga tgtccgagct ctcggaaggc ttcatcgccc
     2761 tgccaggcgg caccggtacg ctggaggaat tcttcgaggt gctgacctgg gcgcaactcg
     2821 gcgagcacga gaaaccgtgc gggctgctca atctggcaag ctactacgac cccctggtcg
     2881 ccctcttcga ccacatggtg gcaaagggtt tcctgagcga ggagcacagg gcgacggtcc
     2941 tggtcgagac cgagccagaa cccctgctcg acgccttcgc ccactacagt ccgccaaaca
     3001 ccgtcaagtg gatagataag gaagagacct agcgccggga cgacgcgcct tccggcacag
     3061 ccaccaaaaa aggcgcccgc gaggcgccaa gagaatctgg cagggggtcg ctaccccgga
     3121 accacggaca ccctgtcggc gcgcaccacg tacctgacgt accagttggg gcccatcgtc
     3181 cagtaatcgc cgtaggtctc gatacaggtc tgcagcgaga ccatgtccct gccgggatcc
     3241 gagcttgcca cccacgtctc gcttggcaac acctcgaaga tctccgtaac ctcgtaggtg
     3301 taggtagtcc cgttggcgtc gtagaggtag atcttgtccc cgtaatccag gagcggcagg
     3361 ttgtagaact gatggtcgct tgccgtgcca ggccagccga gcctgtgcgc ggctatgtag
     3421 gtgttagcgt ggctctccca cggaaagccc gactccacgc gcttggccgc gccggcgtct
     3481 agcgccgcat gagcgttgga gttgctgacg tagttgtcgt agagacccag cttggggatc
     3541 gtcatccaga ggtcgttcgt ggcaggtacg gaggcgacgg gcttctcggc ctcagcctgc
     3601 tcgcgctgcg tatcctcctc acccgactcg gcgtccttcc gcgagctcgt ctgctcctcg
     3661 gcggcctgcc cctgctcggc cacggcttcc ggagccccgc cgatcatcgg taccgcaacc
     3721 ctctcgaccg ataccgtctc gctagccctt tgaaacgggt ccgtgcggaa gaacagcgtg
     3781 atacccagga cgatgattag taacgatgct aacatagcga agcccgcccg ctgacgcctt
     3841 tttctgaact tgcgcatctg cgaacgattc cccatgcgag ggattctaac aatagatttt
     3901 cgcgagtggt agggggtaca ctgtatattt cttaagattt gtgaagacca cgctctgtag
     3961 tggtctgagg agaacgtgga cacaagatgt cctgcaggga cggggtagga cagcttgcgt
     4021 ggagtcactg gagcggcacg aggagctggt gcggggatgt tcgctgtgtt tcgcgggcgg
     4081 gggcaacacg ctggtggtag acgtgccgga ggatgtaagg gtgctgcgtt cctggcaagg
     4141 cgccgggtgg cggggacagg gcgccatcta gggcgattat cgccaactgc tggctgcagc
     4201 tgtcgcgcga gctcccgctg ctgcggcccg aggtcgtgat ccttgtcggg ggattggtcg
     4261 tgagggagtt gctcgggata gggtggctct ccgaagccgt cggcgagact attccgcgtg
     4321 atggggtagt atatgtgcct ctaccgcatc catctggggc ttcgacgtgg ttgaacctgc
     4381 cggagaacag aggcctcctc tttcgcacgc tcgcggtgtt gggaaggctg gtagccgacg
     4441 gtagaatcgg agggtccggc gctcgagcac ggaggctaaa gagttgagcc aaacccagtc
     4501 acggacggcc acgctggtgg cccgggacct caagaagacc tacaacggct tcgaggccgt
     4561 aaagggcgtg gacttcgagg tgcacaaggg cgagtgcttc gggttcctgg ggccgaacgg
     4621 agccgggaag accacgacga tgaagatgat ctacggtgcg gtcataccga gcggaggaga
     4681 actcgaggtt gcgggcttag acgtcacgag gtgtgagcgg gagatcaaac gccgcatcgg
     4741 ggtcgtcccg caggagaaca acctggacga cgacctcaag gtcgaggaga acctgctcgt
     4801 ctacggtagg tactacgatc tgccgcgcaa ggtggcgctg cagcgggccg aggagctact
     4861 cgacttcgtg gagctcacag agaaggcaga gtcgcaggtg gagcagctct ctggc
//
LOCUS       DASTUV010000007         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_571023, whole genome shotgun sequence.
ACCESSION   DASTUV010000007 DASTUV010000000
VERSION     DASTUV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571023"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..676)
                     /locus_tag="VFT03_00135"
     CDS             complement(83..676)
                     /locus_tag="VFT03_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU4846576.1"
                     /translation="MSSFAFGLLFGMATAFPIGVQSFVVLNQGLLVGYPRVFVGIVTA
                     SCCDTLLILLGAAGVSALLNAPGSREVLILIGAIFLVVMGILALRGSSDDEKVEGLMR
                     PAAMVAQTVGVSLLNPHAILETVGVLGAAIAAQDAGNRPEFAAGVVSASWVWFLMIGL
                     GASILQRWLTSPVRLWIQRGSGVLMLALAGVLALELV"
     gene            complement(673..1071)
                     /locus_tag="VFT03_00140"
     CDS             complement(673..1071)
                     /locus_tag="VFT03_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEU4846577.1"
                     /translation="MWGRHLVLDCSSCDRQAVRDPEAIRAFCADLIPSIGMVAYGEPV
                     LEHFATHLPEAAGYSLVQLIETSAVTGHFCDASGDAYIDVFSCKDFDPEMAIRIVYKH
                     FSPKLVRQTDLYRDAPRLPDGTDLRFEEAS"
     gene            complement(1131..1397)
                     /locus_tag="VFT03_00145"
     CDS             complement(1131..1397)
                     /locus_tag="VFT03_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEU4846578.1"
                     /translation="MDQREVVIYTRDRCLSCWRAKRLLWHKGYAYDVVDTTHDHALRF
                     RLARFTGRTTVPYVFVDGRPVGGFAEIKALHRSGALDRLARGEV"
     gene            complement(1450..1923)
                     /locus_tag="VFT03_00150"
     CDS             complement(1450..1923)
                     /locus_tag="VFT03_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4846579.1"
                     /translation="MGEETILLVEDNPNDEILTVRALRKIGLAGEQIAVARDGVEALD
                     YLFGASASRRSLPRLVLLNLKLPRMDGLEVLRRLRTDERTRLLPVVILTSSREQHNMI
                     DCQLADGWILRGVDFDLFTREVDRLRPLLKGDPPERRANLMASLRRRFPQETPCG"
     gene            complement(2178..2375)
                     /locus_tag="VFT03_00155"
     CDS             complement(2178..2375)
                     /locus_tag="VFT03_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846580.1"
                     /translation="MLESCFCGHSGELEDREPVLDAEARWLLRCPSCGHLDDLAWLGE
                     ETALLIWGEAVDRRGDASEAA"
BASE COUNT          423 a          832 c          733 g          442 t
ORIGIN      
        1 tcggatagtc cggggggtct cgagggatga tggcatctgc gtgtcagcaa agcgtctcct
       61 ggagtcatgc ctcggtttcc cttcagacca gctcgagcgc caggacacca gcgagggcca
      121 gcatcagcac ccccgaaccg cgttggatcc agagccttac cggcgatgtc aaccagcgct
      181 ggaggatcga ggctccgagc ccgatcatga ggaaccagac ccacgaagcg ctcaccaccc
      241 cggcggcgaa ctcgggccga tttccggcgt cctgggcggc gatagctgcg ccgagcacgc
      301 cgaccgtctc gaggatcgcg tgtgggttga gcagcgagac gccgaccgtc tgggccacca
      361 tggcggctgg gcgcatgaga ccctcgacct tttcgtcatc ggaggagcct ctgagcgcga
      421 gtatgcccat gacgaccagg aaaatggcgc cgatcaggat gagcacctcc ctgctgccgg
      481 gggcgttgag gagcgccgag acccctgccg ctcccaacag gatcagcagg gtgtcgcagc
      541 aactggctgt cacgatgccg acaaagacgc gggggtaacc gacgagcaga ccctggttca
      601 gcaccacgaa gctctgcacc ccgatcggga aagccgtcgc cataccgaag aggagtccga
      661 aggcgaacga gctcacgatg cctcttcgaa ccgcagatct gtcccgtcgg gaagcctggg
      721 agcatcacgg tacaggtctg tctgccgcac cagcttcggg ctgaagtgct tgtagacgat
      781 ccggatcgcc atctcagggt cgaagtcctt acacgagaag acgtcgatgt aggcgtcccc
      841 gctcgcgtcg cagaagtggc ctgtcaccgc ggatgtctcg atcagctgca ccagggagta
      901 gcccgccgcc tcgggcaggt gggtcgcgaa gtgctccagt acgggttccc cgtaagccac
      961 catgccgatg gatgggatca ggtccgcaca gaacgcccga atggcttcgg ggtcgcggac
     1021 cgcctgacgg tcgcaggacg agcagtccag caccaggtgt cttccccaca tacctatcat
     1081 ccctctctcg agtgttgtcg tgtgttacgg tccggtgtgc cctgcacctc ctacacctcg
     1141 ccgcgcgcca ggcggtcgag cgctcccgat ctgtggaggg ctttgatttc ggcgaagccg
     1201 ccgaccggac gcccgtccac gaagacgtat ggcacggtgg ttcggcccgt gaatcgtgcc
     1261 agccggaagc gcagggcgtg gtcgtgggtc gtgtccacga catcgtacgc gtatcccttg
     1321 tgccagagga gacgtttcgc acgccagcag gaaaggcatc ggtcacgtgt gtagatcact
     1381 acctctctct gatccatcct gatctcacct cctctccact cctgccacgc ggacgctccg
     1441 ccgcgatcct cacccgcacg gtgtctcctg cggaaatctc cgccgcaggg atgccatgag
     1501 gttggccctt cgctccggag gatcaccctt gagcaatggc ctcagccggt ccacctctcg
     1561 cgtgaacagg tcgaaatcca cgcccctcaa gatccagccg tctgcgagct ggcagtcgat
     1621 catgttgtgt tgctccctgg aggaggtcag gatcaccacg ggcaacagac gcgtgcgctc
     1681 gtccgtcctc aggcgcctca acacctcgag gccgtccatt ctgggtagct tgagattcag
     1741 caacaccagc ctcgggagcg accggcggga agccgaggcc ccgaagaggt aatcgagagc
     1801 ctccacgccg tcgcgggcta cggctatctg ctcaccggcc aggccgatct tcctcagggc
     1861 cctcacggta aggatttcgt cgttggggtt gtcctccacc aggagtatgg tttcctctcc
     1921 catgccgtct cggttcctcc ttcgcctcgc ggttcctgcg tggtcacgtg gttccggtct
     1981 tcaccccccg acgaagcgtc gcaccttgcc gacgatctcc tcctcgacgg gtgaggccgt
     2041 cgagagcgcc cgagtgatcc ccgaaaggtt ccacttctgc tgatcggcct gtttggtagg
     2101 tcgcctctat ccgctcgaac atccgctctt ccatctcccg cgccggtacg gccccaccgc
     2161 tgatccgcat ggcctcgcta cgcggcctcg gacgcgtcac cgcgcctgtc caccgcttcg
     2221 ccccagatca ggagcgccgt ctcttcgccc agccatgcga ggtcatcgag gtggccacaa
     2281 ctggggcagc gcagtagcca ccgcgcttcg gcgtccagga ccggctcccg gtcctccaac
     2341 tcgccgctgt ggccgcaaaa acaactctct agcatcgtgc gcctcgctcc cgtcggtggc
     2401 tcgtctcatg agtcatacgg tcagcgtaga
//
LOCUS       DASTUV010000008         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1714162, whole genome shotgun sequence.
ACCESSION   DASTUV010000008 DASTUV010000000
VERSION     DASTUV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714162"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1085
                     /locus_tag="VFT03_00160"
     CDS             276..1085
                     /locus_tag="VFT03_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4846581.1"
                     /translation="MSHVVLRISAGFLVGALVLLVTALSLSEYYLDEGRRLSAAGDTE
                     GAIDASRKAARLDPFDAEPLETQSFLFEQQEHYENAASALRAAIARDPHNYVPYLMLG
                     NLQFNEFDDPEAAEESYREVLRLNPKENTIRDGLAQVLIEQGKLGEARDEYEKLREEE
                     ELSFQGLYDLGRIYVRTGKPEEGQQAIEHAKSRAESRLDELEGPLKEQREQLIVSMQL
                     AIADALVVQGRYAKAREIIAQSSSPQAPALLQLLNSNPDAYRESVVTSEIY"
     gene            1072..1797
                     /locus_tag="VFT03_00165"
     CDS             1072..1797
                     /locus_tag="VFT03_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEU4846582.1"
                     /translation="MRSISRSGWQRERRDDLPLSEILHIVWGRRLLVVGIVLALVLLA
                     LLYGLSREPVYTAKAMVKIEPRERLVSDQEKEAFVEEVRGSVVATDGFIQDVMRRAGW
                     EAATTDFKNRLDFETITQGDETGMLVRFSGSDPEQAARAANAYAGLFVERVERLNEDR
                     LSGGTLAADAVVEHRAAPPSSLRLLLYAAAAVVAGVLAGGAGALLLEGRARSWRGAHD
                     AELTLRAPVLGVIPDYSSVGEED"
     gene            1799..2449
                     /locus_tag="VFT03_00170"
     CDS             1799..2449
                     /locus_tag="VFT03_00170"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEU4846583.1"
                     /translation="MGLGAIRGAGAGLLLDPGSPVRPYYEDLAKNLLSLGEGAKSVVV
                     TSAEPGAGCTSVCLGLGAALAGMGHRTAVVDCNLENPHLHRMLGEPNFVGLTSGLRGE
                     RTLDHYGHDAVPGLLVVPTGPVPTDSAALESGRFVELVRGLEAGREAVILDAPVAGRV
                     LRSRNLSKGFDGILLVVHASRTSKSTARATTDDLLDAGINLLGVVLNGSSRQEVRS"
     gene            2446..>3494
                     /locus_tag="VFT03_00175"
     CDS             2446..>3494
                     /locus_tag="VFT03_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846584.1"
                     /translation="MKERVEPPGDAVSGAPTRGDEVEKSRRTGRLLAIFKAVILLTLA
                     AVTAYGLLNHGLYAEPLWLPVANGILALLLVTVFVRDYYADVPQAAWIMVAFLAALVA
                     IKGLSMAWSISETETINEVVRSSTYLAVFVMALGALTSPRQVGPLMDAAVLMVAAVAG
                     YGLLQKINPVEYPIDSLDGVRIDSTLEYVNTTAAILAMGVVLALARMTTVRNALLRGI
                     YAALTLAFLVALYLTASRGGIGSPGVGLIVLFVLTEKRVQTLGNLLLLSVPGAWLLWR
                     IRDLEGFMNTYLSDKQRMVLGTTFRDDLVISLVAAFVLQAGFVLLSNRYELMPSARRV
                     LSALVVGVTVLVIGAG"
BASE COUNT          560 a         1085 c         1226 g          623 t
ORIGIN      
        1 cgcttcggct ggtggcggtg gtggcggcgg cggctccgct tcggcggcgg ctggtggcgc
       61 gctcccgccc acgggtggtg catcgctgtt ggcgctcggc gcgggtgccc tgcttgtggg
      121 tggaggactt ctggcccgca ggatcgttcg gtagaaacta accgagcagc gcattagaag
      181 aagcgggtcc cttcgggggc ccgcttcttc gtgctcgcgt gggcgccttg tattcggctg
      241 gtgttgtgga tatcctcatg cgcggtgagt aggtcttgag ccatgtagtg ttaagaatct
      301 ccgcgggttt cctcgtaggg gccctcgtcc tgctggtgac ggcgctctcc ctctctgagt
      361 actacctcga cgaggggcgc cggctctctg ctgccgggga cacggagggc gcgatagacg
      421 cttcgcggaa ggcggcccgc ctcgacccct tcgacgccga gccgctcgag acgcagtctt
      481 tccttttcga gcagcaggaa cactacgaga atgccgcgtc cgccctgagg gcggcgatag
      541 cccgggaccc gcacaactac gtaccgtacc tcatgctggg caacctccag ttcaacgagt
      601 tcgacgatcc cgaagcggcc gaggagagct accgcgaggt gctcaggctc aaccccaagg
      661 agaatacgat tcgcgacggt ctggcgcagg tgctcataga gcaagggaag ctgggggagg
      721 cgagggacga gtacgagaag ctgagagaag aggaagagct ctcgttccag ggcctctacg
      781 atcttggcag gatctacgtg cgaaccggta agcccgaaga gggccagcag gccatagagc
      841 acgccaaaag cagggccgag agccggctcg acgagctcga agggcccttg aaagagcagc
      901 gggagcagct catagtgtcc atgcagctcg ctatcgccga cgcgctcgtg gtccagggcc
      961 gatacgccaa ggcgcgcgag atcatagcgc agagctcatc cccgcaagcg ccggcgcttc
     1021 tgcagctcct gaactcgaac ccggatgcgt accgcgagtc ggtggtgacg agtgagatct
     1081 attagccgga gcggctggca gagggagcgg cgggacgacc tcccgctctc cgagatactg
     1141 cacatcgtgt ggggccggcg cctgttggtc gtcgggatag tgctagctct cgtgctgctg
     1201 gcgctgctct acggcctgtc cagggaacct gtctacacag ccaaggcgat ggtgaaaata
     1261 gagccaaggg agaggctcgt cagcgaccag gagaaggagg cgttcgtaga agaggtccgc
     1321 ggctccgtcg tggctaccga cggcttcata caagatgtga tgcgccgggc tggctgggag
     1381 gcggcgacaa cagatttcaa gaaccggctc gacttcgaga ccataacgca gggcgacgag
     1441 acggggatgc tggtacggtt ctcgggctcc gatcccgagc aggccgcccg cgcggcgaac
     1501 gcttacgccg ggctcttcgt ggagagggtc gagcggctca acgaggaccg gctctctggg
     1561 ggaaccctgg ccgcggacgc ggtagtcgag cacagggccg cgccgccatc tagcctccgc
     1621 ctcctgctct acgctgcggc ggccgtcgtg gccggggtgc tggcgggggg agccggggcg
     1681 ctgttgctcg agggcagggc ccgcagctgg cgtggcgcgc atgacgccga gctcacgctg
     1741 cgggcgccgg tgctcggcgt cataccggat tactcatccg tcggagagga agactgagtt
     1801 gggtcttggc gcgataaggg gcgcgggggc cgggttgttg ctcgaccccg gctcgcccgt
     1861 gaggccctac tatgaggacc tggcgaagaa cctcctctct ctgggggagg gcgccaagag
     1921 cgtcgtcgtc accagcgccg agccgggggc tggctgcacg agcgtatgcc tcggcctggg
     1981 cgcggcgctg gccggcatgg gacaccggac agccgtcgtc gactgcaacc tcgagaaccc
     2041 gcacctgcac aggatgctcg gcgagcccaa cttcgtcggc ctcaccagtg gcctgagggg
     2101 tgagaggacc ctggatcact acgggcacga cgccgtgcca gggctcctgg tcgttcccac
     2161 gggtcccgta cctaccgact ccgctgcgct cgagagcggg cggttcgtcg agttggtgcg
     2221 cggcctcgag gcggggcgcg aagcggtgat cctcgacgct ccggtagccg ggagagtcct
     2281 gaggtccaga aacctctcca agggcttcga tgggatcttg ctcgtcgtgc acgcctcccg
     2341 tacctccaag agcacggcgc gcgcgaccac cgacgacctg ctcgacgctg ggataaacct
     2401 cctgggcgtg gttttgaacg gatcgtcgcg ccaggaggtg cggtcgtgaa ggagcgggtg
     2461 gagcctcctg gcgacgcggt gagcggggct cccacgcggg gagacgaagt ggagaagagc
     2521 cgccgaacgg gacgcctgct cgctatcttc aaggccgtca tcctgctgac gctcgccgca
     2581 gtcaccgcct acgggttgct caaccacggg ctctatgcag aaccgctgtg gcttccggtg
     2641 gccaatggca ttctggcgct gctgctcgtg acggtgttcg tgcgcgacta ctacgcggac
     2701 gtgccgcagg cggcctggat catggtcgct ttcctggctg cgctcgtggc tatcaagggg
     2761 ctctccatgg cgtggtccat aagcgagacc gagacgatca acgaggtcgt tcgctcctct
     2821 acgtacctgg cggtcttcgt catggcgctt ggcgcgctca cctcgccgcg ccaggttggg
     2881 ccgctgatgg acgccgccgt cctcatggtc gctgcggtcg ctggctacgg gctgttgcag
     2941 aagatcaacc cggtcgagta cccgatcgac tccctcgacg gcgttaggat agattcgacc
     3001 ctggagtacg tgaacacgac cgccgccata ctcgcgatgg gtgtcgtgct ggcgctggcg
     3061 cggatgacca cggttcgcaa cgccctcttg cgggggatct acgccgccct gacgctagcc
     3121 ttcctggtgg cgctctacct caccgcctcg cgcggcggca tcggctcgcc cggggtgggt
     3181 ttgatcgtcc tgttcgtcct gaccgagaag cgcgtgcaga cccttggcaa cctgctcctc
     3241 ctcagcgttc ccggcgcctg gcttttgtgg cgaatccgag acctcgaggg gttcatgaac
     3301 acatacctct cggataagca gaggatggta ctcgggacga ccttcaggga cgacctggtc
     3361 atctccctgg tggcggcttt cgtcttgcag gccgggttcg tcttgctgtc gaaccgctac
     3421 gagctgatgc cttccgcacg aagggtgctc tctgcgctcg tcgtcggcgt gacggtgctc
     3481 gtgatcgggg ccgg
//
LOCUS       DASTUV010000009         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_856455, whole genome shotgun sequence.
ACCESSION   DASTUV010000009 DASTUV010000000
VERSION     DASTUV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856455"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..499)
                     /gene="ybeY"
                     /locus_tag="VFT03_00180"
     CDS             complement(89..499)
                     /gene="ybeY"
                     /locus_tag="VFT03_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4846585.1"
                     /translation="MPFTVAVLDEAGYGALDPELATRLAATAFYCRGFDVERLGEVSV
                     ALVDPGVIRDLNSRFRGKDEPTDVLSFEVDGPYGEMVGEVVICPACAEMRVEELIVHG
                     ALHLSGMDHGEDFEGSEMARAQEAVIRVARGEQE"
     gene            complement(514..2109)
                     /locus_tag="VFT03_00185"
     CDS             complement(514..2109)
                     /locus_tag="VFT03_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU4846586.1"
                     /translation="MKLGKQVPKTPLGRRVDELHRPPTRFERFREWIEGLPKVRLFVA
                     LILITWLALTALIGLGPRPPIERIIAEGIVERGDLAQLESSNLIRNPDLWKSLIGVGL
                     VVATEMGIIGYFLERFGRRVLKANSAIKLVLAASLMILFTALARLFIELNFNPYLTPL
                     AGLSIIGTMLLGPRIMFPVVVVTSINVGIMSGNDFLLTAALLLGSGFAIYTVVRVRSR
                     QQLLKAGLFIALITAAVTFAVGLLGGGTPSEALWQGLLGLVNGLLSLMLAMVLLPLLE
                     DAFNILTPMKLLELSNTGNTLLHKLLQKAPGTFTHSMQVGSLVDAAAERVGADPLLAR
                     VGAYYHDIGKMEHPAYFIENQIAQVNPHATLSPALSAKVIKRHVKDGLEIGRAWGLPQ
                     EILDHIAQHHGSTRIEYFYRKALEEALDDSEVNEADFRYSSGLPHSKEAGILMLADSI
                     EATVKALPKPTPKRIEDVVADTIKRKLEDGQFDESDLTMHDIHEAGEAIREALIGFLG
                     PRIEYPEAPTDKTAAHKAAGDAT"
     gene            complement(2132..3037)
                     /locus_tag="VFT03_00190"
     CDS             complement(2132..3037)
                     /locus_tag="VFT03_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU4846587.1"
                     /translation="MLEVFGERDSVLRRVEELVECEVIVRGNEIMLRGTEAAVDRAEA
                     VFDGLIGLAEDGDNPTPETAERLYKMADSGGGREVLGDVILTHRGRRVAPKTRNQKAY
                     TDAIRENQIVFGIGPAGTGKTYLAVALAVEALNRGEVARIILTRPAVEAGESLGFLPG
                     DVMAKVDPYFRPLYDALYEMVDPAKFQAHLERGIIEIAPLAFMRGRTLNDAFIILDEA
                     QNTTPQQMKMFLTRLGFGSKMVITGDVTQIDLPKGRVSGLQDARRTLTGVEGVSFVGL
                     RRDDIVRSDLVMRIVDAYEGAREGA"
     gene            complement(3193..3636)
                     /locus_tag="VFT03_00195"
     CDS             complement(3193..3636)
                     /locus_tag="VFT03_00195"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU4846588.1"
                     /translation="MSLAEKIQRDTKAAMKQRDRARVGALRMLGAALKNGEIEAGRPL
                     TEEEERVVLRRQLKQREESAEAFSRAGREEQAASESAEAEIVRGYLPEPLSREELEEI
                     VDRAMQETGASGMNDMGPVMGRAMALAGGRAQGRELAPLVRERLK"
     gene            complement(3633..3977)
                     /locus_tag="VFT03_00200"
     CDS             complement(3633..3977)
                     /locus_tag="VFT03_00200"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEU4846589.1"
                     /translation="MSEKDCIFCRIVRGEIDAEVVHDEDEVLAFNDMRGLAPVHVLVI
                     PKQHLGSLEEITQLPERVARRLLEVCSEVAEKLGVRESGYAVRINNGPDAGQGVFHLH
                     LHVLGAKTLEMP"
BASE COUNT          657 a         1381 c         1228 g          803 t
ORIGIN      
        1 gttccgctgg gtgcggaccg cgtagattag cccgcggtag gcgtggtcga agctgcgagc
       61 taagccctgc tggcctttga gggggggctt actcttgttc gccacgcgcc acccttatca
      121 cagcctcctg cgcgcgggcc atctcgctcc cctcaaagtc ctccccgtgg tccatccccg
      181 agaggtgcaa tgcaccgtga actatcagct cctcgaccct catctcagcg caggcggggc
      241 agatcactac ctccccaacc atctccccgt acggcccgtc aacctcgaag ctgagcacgt
      301 cggtcggctc gtccttaccc ctgaatctcg agttgaggtc gcggatgacg ccagggtcca
      361 cgagggctac cgagacttcg ccaaggcgtt cgacgtcgaa gccgcggcag tagaaggccg
      421 tggcggcgag ccgggtggcc agctcggggt cgagtgctcc gtagccggct tcgtcgagga
      481 ccgcgacggt aaagggcatg agccgccgcg ctgtcaggtg gcatccccgg ccgccttatg
      541 agcggcggtc ttatcggtgg gagcctcagg atactcgatg cggggaccga ggaagcctat
      601 tagggcctcc cggatcgcct cacccgcctc gtggatatcg tgcatggtga gatcgctctc
      661 gtcgaactgg ccatcttcga gctttctctt tatggtatct gcgacgacgt cctcgatgcg
      721 cttcggggtg ggcttcggga gagctttgac ggtcgcttca atcgagtcgg caagcatcag
      781 gatgccggcc tctttgctat ggggcaggcc gctggagtag cggaagtccg cttcgttgac
      841 ttccgagtcg tcgagggcct cttcgagggc cttgcggtag aagtattcga tgcgggtcga
      901 gccgtggtgc tgggcgatgt ggtccaggat ctcctgcggc aggccccagg cgcgcccgat
      961 ctcgagcccg tccttcacgt ggcgcttgat gaccttcgcg gagagggccg gcgagagggt
     1021 cgcgtggggg ttcacctggg cgatctggtt ctcgataaag taggcgggat gctccatctt
     1081 gcctatgtcg tggtagtagg ctcccaccct ggcgagcagc gggtcggccc cgacccgctc
     1141 ggcggcggcg tccacaaggc tgccgacctg catggagtgg gtgaaggtac ctggggcttt
     1201 ctggaggagc ttgtgaagca gggtgttgcc ggtgttggag agctcgagga gcttcatggg
     1261 cgtaagtatg ttgaaggcgt cctcaagcag cggcagcagc accatcgcga gcatcagcga
     1321 gagcagcccg ttcacgagac ccagcagacc ctgccacagc gcttcggagg gggtaccgcc
     1381 ccccaacagg cccaccgcga acgtcactgc ggctgtgatc agggctatga acaggccagc
     1441 tttcagcagc tgctggcgcg aacggacccg cacgacggtg tagatcgcga agccagagcc
     1501 gagcaggagc gccgcggtca ggaggaagtc gttgccgctc atgatcccga cgttgatgct
     1561 cgtgaccacg accacgggga acatgatgcg cggccccagc agcatggttc cgatgatgga
     1621 taggcccgcc agcggcgtca ggtaagggtt gaagttcagc tcgatgaaga gccgggcgag
     1681 cgcggtgaac aggatcatca gagaggcggc gaggaccagc ttgatcgctg agttcgcctt
     1741 gaggacgcgg cgcccgaagc gttccaggaa gtatccaatt atccccatct cggtcgccac
     1801 gacgagaccc accccgatca gcgacttcca gagatcaggg ttgcggatca ggttgctaga
     1861 ctcgagctgt gcgaggtcgc cgcgctccac gatgccctcg gcgatgatcc gctctatagg
     1921 tggacgcgga cccaggccga tcagggcggt gagcgcaagc caggtaatca ggatgagcgc
     1981 gacgaacagc ctcaccttcg gcagtccctc gatccactcg cggaagcgct cgaaccgtgt
     2041 cggcggccgg tgcagctcgt ccactcttct cccgagcggc gtcttcggaa cttgttttcc
     2101 cagcttcatg gctttccccg tctcctgggc cctaggcccc ttccctagcg ccttcgtagg
     2161 catctactat acgcatgacg aggtcgctgc ggacgatgtc gtctcgcctc aagcccacga
     2221 aggagacgcc ctccaccccg gtaagcgtcc tcctggcgtc ctgcaagccg ctcacgcggc
     2281 ccttggggag gtcgatctgc gtgacgtcgc ccgtgatcac catcttggag ccgaacccga
     2341 ggcgcgtcag gaacatcttc atctgctggg gggtcgtatt ctgcgcctca tcgaggatga
     2401 tgaacgcgtc gttcaaggtc cggcctctca tgaacgcaag cggggctatc tcgattatac
     2461 ccctctcgag gtgcgcctgg aacttcgccg ggtccaccat ctcgtaaagg gcgtcgtaga
     2521 gcggccggaa gtacgggtcc accttggcca tcacgtctcc cggcaggaac cccagagact
     2581 cccccgcctc gaccgccggc ctggtaagga tgatcctggc cacttcgccc ctgttgagcg
     2641 cctcgacggc gagcgccacc gcgagatagg tcttgcccgt cccggcgggt ccgataccga
     2701 agacgatctg gttctcgcgg atggcgtcgg tgtaggcttt ctggttacgg gtcttgggcg
     2761 ccacgcgcct gcccctgtga gttaggatca cgtccccgag cacctcgcgt cccccgccgc
     2821 tgtccgccat tttgtagagg cgctcggcgg tctccggtgt cgggttatcc ccatcctccg
     2881 cgagcccgat gaggccgtcg aagaccgcct cagccctgtc cacggctgct tcggtgcccc
     2941 ggagcatgat ctcgttgccg cgcacgatga cctcgcactc gacgagctcc tcgacccttc
     3001 tcaagaccga gtcgcgctca ccgaagacct cgagcatccg tcctggcggt atgaccagcc
     3061 tcgcttctac ctgtgcgccc gttgtagtgt ccgtcttttc ctccttaccc aggttttagt
     3121 ttctcgttca gcactaccat cgcgaaacct gaaaactcag ccgtcgcttc ccctgcagaa
     3181 caactccatc cttcatttca gtcgctcgcg caccaggggg gcgagctccc ttccctgggc
     3241 gcgtccccct gccagggcca tggcccgtcc catcacgggc cccatatcgt tcatcccgct
     3301 cgcgccggtc tcctgcatcg ccctgtccac tatctcttcg agctcttccc gcgagagtgg
     3361 ctcgggcaga tacccgcgca cgatctcggc ctccgccgac tccgaggccg cctgctcttc
     3421 gcgtccggcc ctgctgaacg cctcagcgga ctcctcgcgc tgcttgagct ggcgccgcaa
     3481 cacgacccgt tcctcttcct ccgtgagagg tcgtcccgct tcgatctcac cgttcttgag
     3541 cgcggcgccg agcatccgca gtgcgcccac gcgagccctg tcgcgctgct tcatggcggc
     3601 ctttgtgtcc cgctgtatct tctcggccag gctcacggca tctccagggt ctttgcgccg
     3661 aggacgtgca ggtgaaggtg gaagacccct tgcccagcat cggggccgtt gttgatccgt
     3721 acggcgtagc cactctccct tacgccgagc ttctccgcca cctcactgca gacctccaac
     3781 agccgtcgcg ctaccctctc cggcagctgc gtgatctcct ccagtgaacc gagatgctgc
     3841 ttggggatga caagcacgtg cacaggcgcc agccccctca tatcgttgaa ggcgagtacc
     3901 tcatcctcgt cgtgtactac ctcggcgtct atctcccctc gcactatcct acaaaatatg
     3961 cagtccttct cgctcacatt caactcctcg ttacgggttt ctcacagatc tctccagcgc
     4021 cgcccgcgcc cgagccaccg ccaccatccc ggcggtctcg ctgcgcaac
//
LOCUS       DASTUV010000010         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1142138, whole genome shotgun sequence.
ACCESSION   DASTUV010000010 DASTUV010000000
VERSION     DASTUV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142138"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1076)
                     /locus_tag="VFT03_00205"
     CDS             complement(111..1076)
                     /locus_tag="VFT03_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEU4846590.1"
                     /translation="MYGYRPTTRLLSMLELLQARGRIDGPELARRLEVGERTVRRYVA
                     MLREMGVPVESERGRYGSYALRPGFRLPPMMFTDEEALALALGLLSARRLGLSGVAPA
                     VEGALAKLERVMPEGLRGRLQAFEETVVPAAVVPPRLPEGEVVVILSEAARGRRRVRM
                     RYQSGPSKETEREFDPYAVLRKEGYWYAVGHDHLRGATRLFRLDRVIEARLLEATFER
                     PPGFDEPEGVLFALAVMPQDRWSVEVLLEATLDEAREQMPAIGVFLEEAEGGVLMRSS
                     TSDLGWMARVLSGISFPFLVKGPPELQEALRRHAAEISRQAERAG"
     gene            1183..1404
                     /locus_tag="VFT03_00210"
     CDS             1183..1404
                     /locus_tag="VFT03_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846591.1"
                     /translation="MNEWWSVEYVRERNAHALEEAEERRLARLIKRSAERPSLRARAA
                     RRLFGFAVALEREETWRAVWERLEAPRHP"
     gene            complement(1469..1780)
                     /locus_tag="VFT03_00215"
     CDS             complement(1469..1780)
                     /locus_tag="VFT03_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846592.1"
                     /translation="MAGRGVKRVAAATALLGAGGLLYAREVETRRVEVRPVELRLPRL
                     APEFDGYRLVRFGDIHLDNWSKPECLHRIVDKVNEQRPDLVAGIDDAMEGKIRLRTRP
                     R"
     gene            complement(1794..>2062)
                     /locus_tag="VFT03_00220"
     CDS             complement(1794..>2062)
                     /locus_tag="VFT03_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU4846593.1"
                     /translation="PYLRAKARADERLRESGLDYTIIRPGSLTDEEETGRIEAAESLG
                     RRGEISRSDVARTFAEALEMESTYGKTFEILAGETPIREALERI"
BASE COUNT          331 a          746 c          653 g          332 t
ORIGIN      
        1 tgctcggggt gctggccgcc gccttcgggg tctggtacac cctcggcgcc cttggtatcg
       61 ttgcctaccc gttcgggtaa ggggggacgt aaatcgtccc tgcgcaatga tcacccggcg
      121 cgttcggcct gacgcgagat ctccgcggcg tgtcgcctca aggcctcctg caactcgggc
      181 ggccccttca ccaggaatgg gaagctgatc cccgatagca cgcgcgccat ccagccgagg
      241 tcggaggtgg atgagcgcat gagcacgccg ccttccgcct cttcgaggaa cactcctatc
      301 gccggcatct gctcgcgcgc ctcatcgagc gtcgcctcca gaagcacctc caccgaccag
      361 cggtcctggg gcatgacggc cagggcgaag agcacgcctt ccggctcgtc gaagccgggc
      421 gggcgctcga acgtggcctc cagcaacctg gcctcgatca cccggtccag cctgaacagc
      481 cgcgtcgccc ctcgcaggtg gtcgtggccc accgcgtacc agtaaccctc ctttcgtaac
      541 acagcatagg ggtcgaactc gcgctctgtc tccttcgaag gcccggactg gtaacgcatc
      601 cgtacccgcc tccgcccccg cgcagcctcg ctgaggatga cgacgacctc cccctccggg
      661 agacgcggag gaacgacggc cgccggaacg accgtctcct cgaacgcttg caaccgcccg
      721 cgcaacccct ccggcatcac cctttcgagc ttcgcgagcg ccccctctac cgcaggagcg
      781 acccccgaga gaccgagcct gcgggccgag agcaggccca gagccagcgc cagagcctcc
      841 tcgtccgtga acatcatcgg agggagcctg aaccccggcc tcaaagcgta ggagccgtac
      901 cttccccgct ccgactcaac gggcacgccc atctccctga gcatcgccac gtagcggcgc
      961 acggttcgct cgccgacctc gagccgccgc gcgagctccg gcccgtctat cctcccgcgc
     1021 gcctgcaaca gctccagcat cgagagcaac ctcgtcgtcg ggcggtatcc gtacaccctc
     1081 ctcaacctcc aaaaacccga gggatccaga gccaatcagg aacaattatg acctgtttgc
     1141 ccggtactct cacatcgtag acgagcgagg gagaagtacg acgtgaacga gtggtggtcc
     1201 gtggagtatg tgcgggaaag gaacgcccac gcgctggagg aggctgagga gcggaggctg
     1261 gcgcggctga tcaaaaggtc ggctgagagg ccctcgttgc gggctagggc ggcgcggcgg
     1321 ctattcgggt tcgcggttgc actggaacgc gaggagacgt ggcgcgcggt gtgggagagg
     1381 cttgaggcgc ccaggcatcc ctgatagcca gggggatcgc gctccgggca ggataggctt
     1441 catcggcgtc ccgtgcgggg cgagcgccct agcggggacg ggtccgtaac ctgatcttcc
     1501 cctccatggc atcgtctatc cccgcgacga ggtcgggtcg ctgctcgttg accttgtcta
     1561 ctattcgatg caggcactcg ggcttcgacc agttgtcgag gtggatgtct ccgaaccgga
     1621 caagccggta gccgtcgaac tcgggtgcca gccgtggcag cctgagctcc acgggcctga
     1681 cctccacccg gcgggtctcg acctcccgcg cgtagagaag gccgcctgcc cccaggagcg
     1741 ccgtcgccgc ggccacccgc ttcacgccgc gaccggccac gtccggtgcc tccctagatc
     1801 ctttcgagag cttcgcggat aggcgtctcg ccggccagga tctcgaacgt cttgccgtac
     1861 gtgctctcca tctcgagcgc ctcggcgaag gtgcgggcca catcggagcg gctgatctcc
     1921 ccccgcctgc ccagggactc ggcggcctcg atcctgccgg tctcctcctc gtcggtcagg
     1981 ctccccggcc ggatgatggt gtagtcgagc ccgctctctc gcagccgctc gtcggcgcgg
     2041 gccttagcgc gcaggtaagg cc
//
LOCUS       DASTUV010000011         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1106244, whole genome shotgun sequence.
ACCESSION   DASTUV010000011 DASTUV010000000
VERSION     DASTUV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106244"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..840
                     /locus_tag="VFT03_00225"
     CDS             553..840
                     /locus_tag="VFT03_00225"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU4846594.1"
                     /translation="MCRNIKTLFNFEPPATKEEIHAASLQYVRKVTGFNRPSRTNEAA
                     FYSAVEDIARISERLLASLETTAAPKDREEEAAKAKARSALRFDAPARSSG"
     gene            complement(837..1001)
                     /locus_tag="VFT03_00230"
     CDS             complement(837..1001)
                     /locus_tag="VFT03_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846595.1"
                     /translation="MAPELGAYLVAFALLGHAAWDAYHYLRDRVVARSYAEFCGVLDL
                     LVGAVVLVIA"
     gene            complement(1027..1443)
                     /locus_tag="VFT03_00235"
     CDS             complement(1027..1443)
                     /locus_tag="VFT03_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4846596.1"
                     /translation="MDDKPFNADLFVRVLRRCGYTNELVRVGDGVEALDFLFGKEDHA
                     GRDVGVKPRLILLDLNMPRMGGLETLRRLRADERTKLLPIVMLTATDNSGDKAEAYRL
                     GINGYVDKLSNVPFPEMVKRIADSWLGLNEPAPTQR"
     gene            1709..1972
                     /locus_tag="VFT03_00240"
     CDS             1709..1972
                     /locus_tag="VFT03_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU4846597.1"
                     /translation="MTDVHVYINRPADKEDADLEDLKESLVKLEHVSEVRFDPSGNVV
                     AVSFEGGEPEQEAIERAVEEAGYEVSRISVRSTFSEGPNLWDI"
     gene            2209..2691
                     /locus_tag="VFT03_00245"
     CDS             2209..2691
                     /locus_tag="VFT03_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4846598.1"
                     /translation="MSIFPTKVVLAIDGSEEASRAAEAAVELCEKTGSELHVVYVGED
                     FYLTAVTDLDMVAQTWVAQEYAESETNFEQIAREVLDAEVEKVEAAGGTVAQAHLRVG
                     RADAEIVDLAEEIEAGLVVLGSRGLGGVKRALMGSVSYSVVRHAHSPVLVVRRQDSAT
                     "
     gene            complement(2816..>3167)
                     /locus_tag="VFT03_00250"
     CDS             complement(2816..>3167)
                     /locus_tag="VFT03_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846599.1"
                     /translation="VGKSAEAVVVLYAEMEEHGVGRGGASLHPHVRAAVRRVERLKEM
                     DRLAPETEGPLEAVEAPQRSEEQESPKSTPKTEDSHETPEHQSAEEQPAEEQPAEEQP
                     QRRRSWWREYFGFD"
BASE COUNT          628 a          938 c          980 g          621 t
ORIGIN      
        1 gttcagacta ttcaggattc tgttcgcgtt gctgttcacc accttgctcg ggccgatcgt
       61 gctagtggcg ctgatagcct tcgtcgccta caggtacttc ttcaccgacc gccgttgacg
      121 ataactcgat ttctcgctcg ccccgagcga aacggcgacc cggtactcct cgtggttcga
      181 cgcctggcaa ggcggccgcc tcgccagcct cgcacgtcac agtgcgatag tactgcgatc
      241 cgctctcatc ctggcattct cgtacgcacc ggaacttcac ttcgaccgct ccttgaacca
      301 cgtcggccac cctatatttg caggaaatac aggaaatctg actctgttgc cgacctgcat
      361 cttgcgtatc attatctgca tgactgatgg gcagttcgca ccggcggcat ggtttggact
      421 gcccgctgta ccatcgacag agacgaggag tagctatgag ctgtaagctt gtttccaccg
      481 cctggtgcac agaagacata ctcatagcga acgggttatg cggtcgttca tctggtctgg
      541 aggccgagtt ctatgtgtag gaacatcaag actcttttca atttcgaacc accggcaacc
      601 aaggaggaga tacacgccgc ttcgctgcag tacgtgagga aggtaaccgg cttcaacagg
      661 ccgtccagga caaacgaagc agccttctac tctgcagtcg aagacatcgc gagaatctcg
      721 gagcggttgc tcgcctcgct cgaaaccact gcagcgccaa aggacagaga ggaagaggcg
      781 gccaaggcga aagcgcgatc ggccttgaga ttcgatgcac cggcgcggtc ctcgggctag
      841 gctattacga gaacgaccgc gccgaccagg aggtccagga caccgcagaa ctccgcatac
      901 gagcgcgcga ccaccctgtc ccgcaggtag tggtaggcgt cccaggcggc gtggcccaag
      961 agggcgaagg ccacgaggta tgcgccgagc tcgggagcca cgaagagcgc cgccagcgcg
     1021 gtcggtctac cgctgtgtcg gggcgggttc gttcaggccc agccaggagt cggcgatacg
     1081 ctttaccatt tcagggaacg gcacattcga caacttgtcg acgtagccat tgatgcccag
     1141 gcggtaggct tccgccttgt cccctgagtt gtctgtcgct gtcagcatta ctatcggcaa
     1201 tagcttcgtc cgttcgtcgg cgcgtaaccg ccgaagtgtc tccaagccac ccatccgtgg
     1261 catgttcagg tccagcagaa tcagtcgggg cttgacgcca acgtcccgac cggcgtggtc
     1321 ctccttaccg aacaagaagt ccagggcttc gacgccgtcg ccaaccctta ctaattcgtt
     1381 ggtatagccg cacctgcgca gaacccgtac gaaaaggtcc gcattgaagg gtttatcgtc
     1441 cacgaacaag ataagcgcat catccacatc caggcacccc tccgatgata tgtcaaacgc
     1501 tgcttccttt ttcttactcg taagttccct gccggccgca ggattaaact gctctagcgc
     1561 tacgcggcgc gttcgcccgg tgcctcgcag ttgcgaggtg aggtttcggg gataatgaca
     1621 gataggagag tcacccgatg aagatgatcc ggatgccaag ccatatcgcg ctctcaggtc
     1681 gaaacggttg atagaaagga gccaatccat gacagacgtc cacgtctaca tcaacagacc
     1741 ggcggacaaa gaagacgcag acctggaaga cctcaaagag tcgctcgtga agctagagca
     1801 tgtctctgag gtcaggtttg atccgtccgg caatgtggtc gctgtatcgt tcgagggagg
     1861 tgagccggag caggaggcga tcgagcgtgc cgtcgaggaa gccggctacg aggtctcacg
     1921 gatttccgtc aggagcacct tcagcgaagg accgaacctg tgggatatct gacgtatctc
     1981 agatgaaatt gcgggccggg tcgcctgtcg ggcgtgcgat ctcctggccg gcgatctgca
     2041 agctcatcac accttcggta tctagacgaa ggcggagacg cttccctcgc cagctcctcc
     2101 ccgcgcgaca gtgggggccg agcccatcgg cggggccggg tacgataatc tgctgggatg
     2161 ctggcaggaa caatgaggct gagagaccga tcctaggggg tagttgggat gagcatcttt
     2221 cctacgaagg tagtgctggc gatcgatggc tcggaggagg cgtcccgcgc ggcggaggct
     2281 gccgtggaac tgtgcgagaa gaccggctcg gagctgcatg tggtctacgt cggggaggat
     2341 ttctacctga cggccgtaac agacctggat atggtggccc agacctgggt cgcgcaggag
     2401 tacgcggaga gcgagacaaa tttcgagcag atagcgcgag aggtgttaga cgcggaggtc
     2461 gagaaggtgg aagcggcagg cggcacggtt gctcaggcgc acctcagggt gggcagagcg
     2521 gacgcggaga tcgtcgacct ggctgaggag atagaggccg ggctggtggt gctgggcagc
     2581 cggggtctgg gtggggtcaa gagggccctg atgggcagtg tctcctactc cgtcgtgagg
     2641 catgcccaca gcccggtcct ggttgtgcgc cggcaagatt cggcgacata aagcgcttcg
     2701 cgcatttcag cattcggggg acgcgggctc tcccgacttc ttgcctggat cgatcatttc
     2761 gagacagaga ggggacgcat ccccaaatag gctatctttc gccacgagaa gccggctagt
     2821 cgaagccgaa atactctcgc caccacgaac gccgacgctg tggctgctcc tccgcaggct
     2881 gctcctctgc aggctgctcc tccgcggact ggtgctcggg tgtctcgtgg gagtcttcgg
     2941 ttttgggcgt ggacttcggg ctctcttgct cctccgatct ctgcggggcc tccaccgcct
     3001 cgagcgggcc ttccgtttcg ggagcaagtc ggtccatctc cttcaggcgc tccacgcgcc
     3061 ggacagctgc ccgcacgtgc ggatgcaagg acgctccgcc acgccctact ccgtgctcct
     3121 ccatctcggc gtagaggacc acgaccgcct cggccgattt cccgacg
//
LOCUS       DASTUV010000012         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_426648, whole genome shotgun sequence.
ACCESSION   DASTUV010000012 DASTUV010000000
VERSION     DASTUV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426648"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VFT03_00255"
     CDS             <1..1309
                     /locus_tag="VFT03_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU4846600.1"
                     /translation="GDNELAAGVEGVLPGETATAWAAHASLKGDRRGIRRLWPFLGPA
                     FIAAVAYVDPGNFATNISAGAQFGYLLLWVILTSNLMAMLIQSMSAKLGIATGKNLPE
                     VCRERFPTFVTLGLWVQAEVIAMATDLAEFIGAALALNLLFGIPLFPAGLMTAVGAFG
                     ILELQRRGFRPLEAAISSLVGVIVIAFAFQMFYAQPEGDRILAGLFTPQFAGTESVLL
                     AAGILGATVMPHVIYLHSALTQRRVVGRTDEEKKKIFRFELVDVVIAMAIAGTINASM
                     LIMAAALFNANGLTGVGDIDKAFEQLNIQVGSTSAVLFGVALLASGFSSSSVGTMSGQ
                     VVMQGFINRSIPLWLRRFITMLPALVILAVGLNPSRSLVISQVVLSFGIPFALIPLLI
                     HCRNRQVMGALVNRRATTAIATVVVALIVSLNVFLLYQTFFGS"
     gene            1438..>2409
                     /locus_tag="VFT03_00260"
     CDS             1438..>2409
                     /locus_tag="VFT03_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4846601.1"
                     /translation="MPETSKKSAVWAWVLYDFANTVFSVSILTVFFPLWVDQQIGHGA
                     WLVNLATAVSALLVFFTVPALGAMADLRQRRVPYLVVLTVISVLLTAGLDLAGGVVVG
                     VALFVAADLTYQSAIVFYNALLPVVAAGRGAGRISGYGTAAGYVGTILALIFLTFFVS
                     DAETVRSLLGPFGGWMETGGEQNSNAFLPTAILFLLFSVPAFFLVPDPAVRPPRPVAL
                     GIAYRDVLNTVRNLRTGYAGVGTFMLATILYMDAANTAVSNMALYSREVFEMDSTEVR
                     NLLLFSTVFAVVGSTASGFATDAIGPKKTLLAVLALWLISIALAAAAV"
BASE COUNT          362 a          813 c          725 g          509 t
ORIGIN      
        1 cggcgacaac gagctcgccg cgggcgtcga gggggtcctg cctggtgaga ctgccaccgc
       61 gtgggctgcg cacgcctcgc tcaaagggga tcgcagaggt attcgcagac tctggccgtt
      121 ccttggtcct gcgttcatcg cggcggtcgc ctacgtcgac cctggcaact ttgccaccaa
      181 catctccgcc ggggcgcagt tcgggtatct gctcttgtgg gtcatcctga cctcgaactt
      241 gatggcgatg ctcatccagt cgatgtcggc caagctgggg atagccacgg gcaagaacct
      301 gccggaggtc tgccgcgagc gcttcccgac gttcgtcact ctggggttgt gggtgcaggc
      361 cgaggtcatc gccatggcca cggatctcgc ggagttcatc ggggcggcgc tcgccctcaa
      421 cctcctcttc ggcataccgc tcttccccgc tggcctcatg accgcagtag gcgccttcgg
      481 aatcctcgag ctccagcgga ggggcttcag gccgctcgag gcggcgatct cttccctcgt
      541 gggggtgatc gtcatcgcct tcgctttcca gatgttctat gcccagcccg agggagaccg
      601 gatactggct ggcctcttca cgccgcagtt cgcaggcacc gagagtgtgc tcctcgcggc
      661 gggcatcctc ggagccacgg tcatgccgca cgtcatatat ctgcactcgg ccctgacgca
      721 gcgcagggtg gttggccgca ccgatgagga gaagaagaag attttccgct tcgagctcgt
      781 tgacgtggtt atcgccatgg ccatcgcagg gaccatcaac gccagcatgc tcatcatggc
      841 cgcagcactc tttaacgcca acggcctaac cggcgttggc gatatcgata aagccttcga
      901 gcaactgaac atccaggtcg ggagcacctc ggccgtcctc ttcggcgtag ctttgctcgc
      961 gagcggcttc tccagctcct cggtcgggac gatgagcggc caggtagtca tgcagggctt
     1021 cataaatcgg agcatcccgc tgtggctcag gcgctttata acgatgctgc ctgccctcgt
     1081 catcctcgcc gtcgggctca accccagccg ctccctggtc ataagccagg tcgtgctctc
     1141 gtttggcatc cccttcgccc tgatcccttt gctgatccac tgccgcaacc gccaggtgat
     1201 gggggcgctg gtcaaccgcc gcgctaccac ggcaatcgcc acggtggtag tcgctctcat
     1261 cgtctccctg aacgtattcc tgctctacca gaccttcttc ggatcctaga gccggggcgc
     1321 ggtgtgttct ttccgctact tcttcgcgct tcacgcgcgg cctatcaaga cggctgtata
     1381 ctcacgccag cgtagtcggc gtgagtatag aaccggaaag ccaatacctg agacctgatg
     1441 cctgagacct cgaaaaagag cgctgtctgg gcctgggtcc tctacgattt cgcgaacacc
     1501 gtcttctcgg tcagcatcct gacggtgttt ttcccgctgt gggtcgacca gcagatcggg
     1561 cacggcgcct ggctggtcaa cctggccacg gccgtctcgg cgctccttgt cttcttcacg
     1621 gtccctgccc tcggggccat ggcggacctg cgccagcgca gggtgcccta cctcgtggtc
     1681 ctcaccgtga tctcggtcct gctaacggca ggcctcgacc tcgcgggcgg ggtcgtggtt
     1741 ggggtcgcgc tgttcgtggc tgctgacctg acataccagt cggcgatcgt cttctacaac
     1801 gcgctgctgc cggtggtcgc ggcggggcgc ggggcgggca ggatcagcgg ctacggcacc
     1861 gccgcgggct acgtcggtac catcctcgcc ctcatcttcc tgacgttctt cgtctccgac
     1921 gcagagaccg tgcgctccct cctcgggcct ttcggcggct ggatggagac cggcggcgaa
     1981 cagaactcca acgccttcct gccgacagcc atcctctttc tcctgttcag cgtgcccgcc
     2041 ttcttcctcg tgcccgaccc ggcggtgcgg cctccgcgtc cggtcgctct gggcatcgcc
     2101 tatagggacg ttctcaacac ggttcgcaac ctgcgcactg gctatgccgg cgtggggacg
     2161 ttcatgctcg ccacgatcct ctacatggac gcggcgaaca cggctgtgtc taacatggcg
     2221 ctctacagcc gcgaggtctt cgagatggac agcacggagg tcaggaacct cctcctcttc
     2281 tcgactgtct tcgcggtcgt aggctctacc gcctccggat tcgcaacgga cgcaatagga
     2341 ccgaaaaaga cgctgcttgc cgtgctcgcg ctgtggctta tttctatagc gctcgccgcg
     2401 gcggcggtc
//
LOCUS       DASTUV010000013         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_999726, whole genome shotgun sequence.
ACCESSION   DASTUV010000013 DASTUV010000000
VERSION     DASTUV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999726"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VFT03_00265"
     CDS             complement(<1..1709)
                     /locus_tag="VFT03_00265"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU4846602.1"
                     /translation="MSVARNLTRKLLADHLLDGDLVAGEEVNLAVDQILLEDALGSMT
                     ALQFEALEPDRVSVPLAVMYVDHNVLQIDDKNMDEHRYLQSFSARYGIHYSRPGNGIS
                     HYVHLERFARPGGLLVGADSHSTMAGALGMFAVGAGGLDVAVAMAGHGFSVECPKVVG
                     VELRGELPAWVQSKDIILELLRRYGVRGGVGRVFEFTGEGVSALSVTDRGTICNMITE
                     LGATAAVFPSDLQTRKWLEAQRREDDYVPLAADSGASYDETEVIELAELEPLIAKPTS
                     PGNVVPVREVAGTVAVQVCVGSSVNSSYEDLATAAAVLRENVVDPRIEMTVTPGSRQI
                     LDTISRSGVYQELVAAGARMLEPVCGPCIGVGQAPSAGVPSVRTFNRNFPGRSGTDGD
                     EIYLCSPATAAATALRGEIADPRELGEPPTITPAPSDPDMDDRQILAPPPPEEARKVE
                     IVRGPNIVPPPEGQPVPEVLEGRVVIVVEDDVSTGDMAPDGALGMSLWSNIPECAKYM
                     FRRQDLGFHDRALEWGGGFIVGGHNYGQGSSREHAALAPLHLGIRAVIAKSFARIHRR
                     NLIS"
     gene            1811..2191
                     /locus_tag="VFT03_00270"
     CDS             1811..2191
                     /locus_tag="VFT03_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846603.1"
                     /translation="MDPILDLERRRLLISAALTAHARRRLGKRLTAGELLDLAVRLKE
                     DAGPVERGYRLRFEPAYPGVTAGPQSIGSTRRLLLACRVHDRDGSPLGVVFTTLIAGR
                     PPQVSVAPPGAAIPGDWRPLRDFH"
     gene            complement(2257..3252)
                     /locus_tag="VFT03_00275"
     CDS             complement(2257..3252)
                     /locus_tag="VFT03_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU4846604.1"
                     /translation="MTVVTGFLGSGKTTLLARVLSEPSMVNTAVLVNEFGEVGLDHHL
                     LRRVDERTVLLGSGCVCCTTREDLVGALMELLDLDQRGEIPRLDRVVIETTGLADPAP
                     ILHTIYADPVLQHHFAVDLVLTTVDAVNGELHLDRNPESVKQVAAADKVVVTKTDIAE
                     PGVARDLLSRLGTINPSARTFEAAFGEVDVDELFRPDGAGAHAPSLAAEPDELHDTGE
                     THSVSVTFDEAIDWTAFGIWLSMLLNARGEDVLRVKGLLDVGEAGPVILNGVQHIIHP
                     PQHLGEWPDEDYRSRIILITRGIHPEELLDSLLAFSGLLGARPLPLHARQSVAFP"
     gene            complement(3284..3637)
                     /locus_tag="VFT03_00280"
     CDS             complement(3284..3637)
                     /locus_tag="VFT03_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846605.1"
                     /translation="MCVLCGQDFVAQAHWTDRHVEDRARASGPGADPVEYQRDRRRDR
                     GHRVVLTNEVLRHYGLRVEDWSGSKYVLRDRKGRSELVQDLGSLWPAAAKLAGRTLDP
                     LDPALCEALSAARGG"
     gene            complement(3654..>4041)
                     /locus_tag="VFT03_00285"
     CDS             complement(3654..>4041)
                     /locus_tag="VFT03_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEU4846606.1"
                     /translation="GEEIDLVKIGGNWYLDDLPPMMFIKASPNSHGFVNPRDIEDLWR
                     DQFDWVYREMDYATYCLTIHPDVSGRPQVLLMLERLIDYINGHDGIRWATMEEIADDF
                     VERYPRSKPNHDPGLNMYGVETPAAE"
BASE COUNT          655 a         1329 c         1339 g          718 t
ORIGIN      
        1 gagatcagat ttcggcggtg gatgcgggcg aagctcttgg cgatcacggc ccgtatgccg
       61 aggtgcagcg gggcgagagc ggcgtgctcg cggctggagc cctgcccgta gttgtgcccg
      121 ccgacgatga aaccgccgcc ccactccagc gcccggtcgt ggaaccccag gtcttgccgg
      181 cggaacatgt acttcgcgca ctcgggtatg ttcgaccaga ggctcatccc caaagccccg
      241 tcgggagcca tgtcccctgt cgagacgtcg tcttcgacga ctatgacgac gcgtccctcc
      301 agcacttcgg gcaccggctg gccctcgggc gggggcacga tgttggggcc gcggacgatc
      361 tcgacttttc gcgcctcctc cggtgggggt ggggccagga tctggcgatc atccatgtcc
      421 gggtcggacg gcgcaggggt tatggtcggc ggctccccca gctcgcgcgg gtcggcgatc
      481 tcaccccgaa gcgcggtcgc ggcggccgtc gccggtgagc agaggtagat ctcgtctcca
      541 tctgtcccgc tgcgacccgg gaagttgcgg ttgaaggtcc tgaccgacgg tacgccggcg
      601 gaaggagcct gtccgacgcc gatgcacgga ccgcagacgg gctcgagcat ccgggcgccg
      661 gccgcgacga gctcctggta gacgccgctc ctcgagatgg tgtcgaggat ctggcgcgac
      721 cccggcgtga ccgtcatctc tatgcgcggg tccactacgt tctcccgcag aaccgcagcg
      781 gctgtggcga ggtcttcgta ggaggagttc accgaggaac cgacgcacac ctgcaccgcc
      841 accgtgccag cgacctcgcg gaccggcacg acgttgcccg gagaggtggg cttcgcgatc
      901 aggggctcca gctccgcaag ctctatgacc tctgtctcgt cgtaggaggc gccggaatca
      961 gcggcgagcg ggacgtaatc gtcctcgcgt cgctgcgctt cgagccattt acgggtctgc
     1021 aggtcggagg ggaacacggc tgcggtcgcg ccgagctcgg tgatcatatt gcagatcgta
     1081 cctcgatcgg tcaccgagag cgccgagact ccttctccgg tgaactcgaa gaccctccct
     1141 actcccccac gaaccccgta gcgtctcaga agctcgagga ttatgtcttt cgactgcacc
     1201 caggcgggca actctccgcg cagctcgacg ccaactacct tcgggcactc caccgagaag
     1261 ccgtgccccg ccatggcgac ggccacgtcg agaccacccg ccccgacggc gaacatccct
     1321 agagctccgg ccatggtcga gtgcgagtcg gcgccgacca gcagcccgcc gggtctggcg
     1381 aacctctcga ggtggacgta gtgggagatc ccgttgccgg ggcgggagta gtggatgccg
     1441 tagcgtgccg agaatgactg caggtagcgg tgctcgtcca tgttcttgtc gtcgatctgc
     1501 aagacgttgt gatccacgta catgaccgcg agcgggacgg agacccggtc cggttcgagc
     1561 gcctcgaact ggagggcggt catggagccg agagcgtcct cgagcaggat ctggtcaacc
     1621 gccagattca cctcctcccc ggctacgagg tctccgtcca gcaggtggtc agccagcagc
     1681 ttcctggtaa gattgcgcgc cacgctcatg ttcgccgcct tcccgtagac aacgcggtac
     1741 ggacgtcatc gctagaatgg tcggctggag catagacgac ccgtctcgta ctggcgagca
     1801 aatcttgcgc atggacccga tcctcgacct cgagaggaga cgcctcctca tatccgcggc
     1861 gctgaccgcg cacgcgaggc ggcgcctcgg gaagagactc actgccggag aactcctgga
     1921 ccttgcggtc aggctgaagg aggacgcggg ccctgtcgag cgcggctacc ggctccgctt
     1981 cgagccagcc tatccgggcg tcacagccgg acctcagagc atcggtagca ctcgcagact
     2041 cctcctcgcg tgcagggtcc acgacaggga tggatctccc ctcggcgtgg tctttaccac
     2101 gctgatcgct ggccggccgc cccaggtgtc ggtggcgccg cccggggcag ctatccctgg
     2161 agactggcga cccctgcgtg actttcactg aattcacccg cccctgagcg tcatggctaa
     2221 tggcccatga gctttaatgc gaagtccatc aaagggttac gggaacgcta cgctttgcct
     2281 ggcgtggagg ggcaggggcc gcgctccgag caatccgctg aaggccagca gggagtccag
     2341 caactcttcg ggatggatgc ctctggtgat gaggatgatc cgggatcggt agtcctcgtc
     2401 gggccactcg ccgaggtgct gcggcgggtg gatgatgtgt tgcacgccgt tgaggatgac
     2461 gggtccagcc tcgccgacgt cgaggagccc cttgactcgc aacacgtcct cgcccctggc
     2521 gttgagcagc atgctgagcc agatcccgaa cgcagtccag tcgatggctt cgtcgaacgt
     2581 gaccgagacg gagtgggtct ctcccgtgtc gtgaagctca tcgggctccg cagccaggga
     2641 tggcgcgtgc gctcccgcgc cgtcggggcg aaagagctcg tcaacgtcca cctcgccgaa
     2701 agcagcctcg aaggtccggg ccgacgggtt tatggtgccg agcctggaca gcaggtcccg
     2761 tgcgacgccc ggctccgcta tgtcggtctt ggttacgacg actttgtccg ccgccgcgac
     2821 ctgcttgacc gactcagggt tgcggtcgag gtgcaactct ccgttgaccg cgtccactgt
     2881 ggtgaggacc aggtccacgg cgaagtggtg ctgcaggacg gggtcggcat agatggtgtg
     2941 gaggatagga gccgggtcag ccagaccggt ggtctcgatg acgacgcggt ccaaccgggg
     3001 tatctcgcct ctctggtcca gatcaagcag ctccatgagc gctccgacca ggtcctctcg
     3061 cgtggtgcag cagacacaac cgctcccgag caggaccgtg cgctcgtcca ccctgcgcag
     3121 gaggtggtgg tcgaggccga cctcgccgaa ctcgttgacg aggacggccg tgttgaccat
     3181 cgacggctcc gacagcactc tcgcgaggag ggtcgtcttg ccgctgccga ggaagcccgt
     3241 cacgaccgtc accggccttc tggtgtgcct atcctccacg gtcttacccg ccgcgcgccg
     3301 ccgagagcgc ctcgcacaga gcggggtcga ggggatccag cgtcctgccg gccagcttcg
     3361 ccgccgcggg ccacagggaa ccgagatcct ggaccagctc ggatctcccc ttgcggtcgc
     3421 ggagcacgta tttgctcccg ctccagtcct ctaccctgag gccgtagtgg cgaagcacct
     3481 cgttggtcag gaccaccctg tgtccgcggt cgcggcgccg gtcgcgctgg tactcgaccg
     3541 ggtcggcgcc gggaccggac gcgcgcgccc ggtcctcgac gtgccggtcc gtccagtggg
     3601 cctgcgccac gaagtcctgt ccgcaaagca cgcacatgga agaggttata cccctattcg
     3661 gcggccgggg tctcgacgcc gtacatgttg aggccagggt cgtggttggg cttggacctg
     3721 gggtagcgct cgacgaagtc gtcggcgatc tcctccatcg tcgcccagcg tatgccgtcg
     3781 tggccgttta tgtagtctat gagcctctcc agcatcagca agacctgcgg tctgcccgaa
     3841 acgtcggggt ggatcgttag acagtatgtt gcgtagtcca tttctctgta tacccagtcg
     3901 aactgatccc gccacaggtc ctcgatgtcg cgcgggttga cgaagccgtg gctattgggc
     3961 gaggccttga tgaacatcat cggaggcagg tcgtcgaggt accagttacc gccgatcttg
     4021 acgaggtcta tctcctcacc c
//
LOCUS       DASTUV010000014         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_820310, whole genome shotgun sequence.
ACCESSION   DASTUV010000014 DASTUV010000000
VERSION     DASTUV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820310"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFT03_00290"
     CDS             complement(<1..547)
                     /locus_tag="VFT03_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU4846607.1"
                     /translation="MAAKRDYYEVLGVSRGTSEAEIKKAYRRLARDHHPDVNSHDPGA
                     EERFKELTEAYEVLSNPESRRAYDTYGHQVPRGASGGYPGGDPFGGFQDIFEAFFGDR
                     GFGGSFFGPTASRAPSRGADVEVEVEVALREAAFGVDREVRVQTVKNCQVCDGIGGTE
                     FHSCPTCGGAGAVRTVRESFLG"
     gene            complement(550..1581)
                     /gene="hrcA"
                     /locus_tag="VFT03_00295"
     CDS             complement(550..1581)
                     /gene="hrcA"
                     /locus_tag="VFT03_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU4846608.1"
                     /translation="MRHPEISDRQREILLKTLELYISTGAPVGSHALTEAVDASSSTI
                     RAELADLESSGLLTHPHTSAGRVPTDAGYRYYVDALMSDHPREGNSFEAPEGYGNLDE
                     LLRGVSEGMSEVTRLLAVVAGPSALGDSLSRVDYLPLVDGALLLVVSMESGATSSTTL
                     TLPTFVEGDELREIFAALNSWLGGRSVGGDLELATAARKVLSDHDPLVVGAVLEAIEV
                     LGRATDRGVFVRGASALLARLDDLDPQSLSAVVEIFERRRWLLRLMGDALERSAVSSS
                     GVVVLIGAENALYNLMGTSLVAAAYGHHQRPYGVVSLIGPKRMDYDAAITTVRSAAET
                     LTHRLSAGF"
     gene            complement(1626..3404)
                     /gene="lepA"
                     /locus_tag="VFT03_00300"
     CDS             complement(1626..3404)
                     /gene="lepA"
                     /locus_tag="VFT03_00300"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU4846609.1"
                     /translation="MVNIERTRNFCIIAHIDHGKSTLADRLLDATDAVPERERVAQIL
                     DTMELERERGITIKAQAVRLLYNRSDGQWTLNLIDTPGHVDFAYEVSRAIAACEGALL
                     IVDASQGIQAQTLANLYLAMEHDLEIIPVINKIDLPVADVPAVTEEIVELLGVDEEEI
                     LKISAKTGEGVRSVLDAILERVPPPATTREETRALIFDSHYDPYRGVVSLARLFDGEL
                     KKGDAVRAMGSDEHFELLEVGCYSPKPTALPALGAGEVGYVIAGLKDIEALRVGDTVT
                     RVNDPASEPLPGYAQALPTVFSGLYPTVGDDFERLREALAKLQLNDASLFFEPENSRM
                     GFGFRCGFLGLLHMEIVQERLEREFDLELIASSPSVKYEVVVDGKVVEITNPSDLPEA
                     FEEIREPVVRASIIAPKEYVGAVMSLCHEKRGVSVGMEYISTRRVQLTYDLPLAEIIT
                     DFFDALKSRTRGYASYDYEPTDYESADLVKVEVLVSGEVVDALSIIVHRDKAYGRGRA
                     LVEKLRELIPRQQFEVPVQAAVGKRIIARETVRAYRKDVTSKCYGGDITRKRKLLEKQ
                     KAGKRRMKQVGSVEIPQEAFLAALRI"
BASE COUNT          616 a         1177 c         1123 g          630 t
ORIGIN      
        1 ggcccaggaa gctctcccgc accgtcctga cggccccagc acccccgcac gttgggcagg
       61 agtggaactc cgtaccaccg atcccgtcgc agacctggca gttctttacc gtctgtactc
      121 tcacctcccg gtccaccccg aacgccgcct cgcgcagcgc tacctcgacc tccacttcga
      181 cgtcagcccc gcggctcggc gcgcgcgagg ccgtagggcc gaagaaggag ccgccgaagc
      241 cgcggtcgcc gaagaacgcc tcgaagatgt cctggaagcc gccaaagggg tccccaccag
      301 ggtaaccgcc agaggctcca cgcgggacct ggtgcccgta ggtatcgtag gcgcggcggg
      361 attcggggtt ggagagcacc tcgtaggcct cggtgagctc cttgaagcgc tcttcggcgc
      421 cagggtcatg ggaattgacg tccgggtggt ggtcgcgggc gagccggcgg taagcctttt
      481 tgatctccgc ctcagatgtt cccctggaca cgcccaggac ttcgtagtag tcgcgcttgg
      541 ccgccacgcc tagaacccgg cgctcaggcg gtgggtcagg gtctcggccg ccgaacggac
      601 cgtggtgatg gcggcgtcgt agtccatgcg cttgggtcct atgaggctga cgacaccgta
      661 gggccgctga tggtggccgt aggccgccgc gaccaggctc gtgcccatca ggttgtagag
      721 agcgttctcg gcgcctatca agacgacgac gcccgagctg gagaccgccg accgctcgag
      781 agcgtcgccc atcagcctga gaagccagcg ccggcgctcg aagatctcca cgaccgccga
      841 taggctctgg gggtcgaggt cgtccaggcg cgccagcaag gccgaggcgc cgcgtacgaa
      901 caccccgcgg tcggtggccc gcccaaggac ctcgatggct tcgagtaccg caccgacgac
      961 tagcgggtcg tggtccgaga gcaccttccg ggcggcggtg gccaactcca gatcgccgcc
     1021 gaccgaccgg ccgccgagcc aggagttgag ggccgcgaag atctcgcgca actcgtcccc
     1081 ctctacgaag gtgggcagcg tgagcgtggt gctggaagtc gccccgctct ccatggagac
     1141 gacgagcagc agcgcaccgt ccaccagagg tagatagtct acccgcgaga gcgagtcccc
     1201 cagagcgctg ggacccgcta ccacggccag gagccgcgtc acctcgctca tcccctccga
     1261 gacgccgcgc agaagctcat ccaggttgcc gtacccctcg ggagcctcga aggagttccc
     1321 ctccctgggg tggtcggaca tgagggcgtc cacgtagtag cggtaccctg cgtcggtggg
     1381 cacgcggccc gcggaagtgt gcgggtgggt cagcagcccg ctcgactcga ggtcggcgag
     1441 ctcggcccgt atcgtggagc tgctcgcgtc aaccgcctcg gtgagggcgt ggctacccac
     1501 aggcgctccc gtcgaaatgt agagctccag tgttttgagc agtatctcgc gctgcctgtc
     1561 ggagatctcg gggtgcctca taacgcgcga attttatcat gatggagatc cggccagaga
     1621 gaagctcaga tgcgcagcgc cgcgaggaag gcctcctgcg gaatctcgac gctccccacc
     1681 tgcttcatcc ggcgcttgcc ggccttctgc ttctcgagca gcttccgctt ccgcgtgatg
     1741 tcgccgccat agcacttgga cgtcacgtct ttgcgatacg ccctcacggt ctcccgggcg
     1801 atgatcctct tccccaccgc ggcctgcacc ggcacctcga actgctggcg tgggatcaac
     1861 tccctgagct tctctacaag cgcccgccca cgcccgtacg ccttgtcgcg gtgcacgatg
     1921 atggagagag cgtccacaac ctcccctgaa accagcacct cgacctttac caggtccgca
     1981 ctctcgtagt ccgtgggctc gtaatcgtag gaggcatacc ccctggtacg gctcttcagg
     2041 gcgtcgaaga agtccgttat gatctccgcg agcgggaggt cgtaggtgag ctgtacgcgg
     2101 cgcgtagaga tgtactccat tcccaccgag acgccgcgct tctcgtggca gaggctcatc
     2161 accgcgccta cgtactcctt cggcgcgatg atcgacgccc gcaccacggg ctcccggatc
     2221 tcctcgaacg cctcgggcag gtcgctagga ttggttatct ctacaacctt accgtcaacc
     2281 acaacttcat acttcacgct cggcgaggag gcgataagct cgagatcgaa ctcgcgctcg
     2341 agtctctcct gcacgatctc catgtgaagg agacccagga agccgcacct gaagccgaac
     2401 cccatccgcg aattctccgg ctcgaagaag agcgaggcgt cgttgagctg cagcttggcc
     2461 agggcctcgc ggaggcgctc gaagtcgtcg ccgacggtcg ggtagaggcc gctgaagact
     2521 gtggggagcg cctgagcgta gccgggcagg ggctccgagg cagggtcgtt caccctcgtg
     2581 accgtatcac cgacgcgcag ggcctctatg tccttgagcc cggcgatcac gtaccctacc
     2641 tcgcctgctc ccagagccgg cagcgcggtt ggcttgggcg aatagcagcc tacctcgagt
     2701 agctcgaagt gctcgtcgct ccccatcgcc cttaccgcgt cccccttctt gagctccccg
     2761 tcgaagaggc gggccaaaga gacgacgccc cggtaagggt catagtggga gtcgaagatc
     2821 agggcccgcg tctcctcccg cgtcgtggct gggggtggga cacgctccaa tatagcgtcg
     2881 aggacgctac gcacgccttc gccggtcttc gcggaaatct tcagaatctc ctcctcgtcc
     2941 acaccgagca actcgacgat ttcctcggtg acggcgggaa cgtcggctac gggcaggtct
     3001 atcttgttga tgacggggat gatctccagg tcgtgctcca tagcgaggta caggttagcg
     3061 agcgtctggg cctggatgcc ctggctcgcg tcaacgataa ggagcgctcc ctcacaggcg
     3121 gctatggcgc gagagacctc gtaggcgaag tcgacgtgac ctggggtgtc tatgaggttg
     3181 agcgtccact ggccgtcgga gcggttatag aggaggcgga cggcctgggc cttgatggtt
     3241 atgccccgct cgcgctccag ttccatggtg tcgaggatct gggccacccg ttccctctcg
     3301 gggacggcgt cggtcgcgtc aagcagcctg tccgcgaggg tgctcttgcc gtgatcaata
     3361 tgtgcgataa tgcaaaagtt gcgtgtacgc tcgatgttta ccacccgtag attttaccat
     3421 gattgcccgt tagctccgta tatgttacat tctcccggtc aggcaagtat caaagcaagg
     3481 ggagcgatca gcgtgcccgc accatccaag cgcgacaagc agagcctcaa gcggttcgag
     3541 cagaac
//
LOCUS       DASTUV010000015         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1249328, whole genome shotgun sequence.
ACCESSION   DASTUV010000015 DASTUV010000000
VERSION     DASTUV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249328"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFT03_00305"
     CDS             <1..769
                     /locus_tag="VFT03_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4846610.1"
                     /translation="NGETQISDASRPIDVADEVPVCEENGVEFIEIPIAYDGISVVVN
                     EQNDWATDVTSEELKTMWEPAAEGKINRWNQVRSSWPDRPLDLYGPGTESGTYDFFNE
                     TIVGNEEEVNRSDYEASEDDNVLVQGVAGDVNALGYFGYSYYENNTDKLNVLAVDGVK
                     PTPDSIRSGEYVLSRPLFIYVSTDALKKNKAVEPFVDFYLDNLDKLVRAAKYVTMPNS
                     LEKEAYAQFEDRTTGTVFNADGEPKGGDLETALKQSQ"
     gene            867..1799
                     /gene="pstC"
                     /locus_tag="VFT03_00310"
     CDS             867..1799
                     /gene="pstC"
                     /locus_tag="VFT03_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU4846611.1"
                     /translation="MAVEAGGTRSQTEVWKRNSRTREARERVIKVLLAACAYLSVFTT
                     FGIVVVLFEETLRFFVEVSPVEFLTSTRWAPQQDAFGILPLVYGTLMATVIAIAVALP
                     VGLLTAIYLSEYAPTSLRRWLKPALEILAGVPTVVYGYFALTFITPFFRESVFPSIGS
                     FNALSAGLIMGVMIIPTVASVSEDAIYAVPQSLREGAYALGATRRETATKIVVPAALS
                     GIVAAVILGVSRAIGETMIVTIAMGSQPNFGGSPLESMQAMTAYIVQVVGGETPRGSI
                     TFKSIFAVGSALFLITLTLNLVSYWFVRRYREIY"
     gene            1820..2710
                     /gene="pstA"
                     /locus_tag="VFT03_00315"
     CDS             1820..2710
                     /gene="pstA"
                     /locus_tag="VFT03_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU4846612.1"
                     /translation="MKRRGESDFKASMSTRYGLDWAFTVLVTVAALIGIIVLVVLVVD
                     VTRDGSSMLSLNFLTSFPSQIFPENGGIYPALIGSLWLLGLTALISVPLGLGAAVYLE
                     EYAQDTRINRIVEVNISNLAGVPSVIYGLLGLGIFVQLLAPVTGGTSVLTGALTLSLL
                     ILPVIIVATREALRAIPASIREGGYALGATKWEVIRSHLLPMALPGALTGIILALSRA
                     IGEAAPILVVGVSLYQTYVTTGPFDGYMALPTQIYDWISRPQQVFQDSAAAGIVVIMV
                     VLLLANSIAIVVRNRYQKRA"
     gene            2744..>3268
                     /locus_tag="VFT03_00320"
     CDS             2744..>3268
                     /locus_tag="VFT03_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4846613.1"
                     /translation="MDTERARRERRVSELSEEQSEVRQRGEGTPVGDGSTLGMKVQDL
                     SAYYGPFKAITDVSMDIQANKVTAVIGPSGCGKSTFIRCLNRMHEVITEARAEGCVML
                     GDKDIYDRKADPVVIRRRVGMVFQKPNPFSKSIYENVAWGARINGFRGDMDELVEQSL
                     RHAALWDEVKDKLKK"
BASE COUNT          612 a          970 c         1084 g          602 t
ORIGIN      
        1 caatggcgaa acacagatct ccgacgcttc caggccgata gacgtggccg atgaggtgcc
       61 ggtgtgcgag gagaacgggg tggagttcat cgagatcccg atcgcctacg acggcatctc
      121 ggtagttgtc aacgagcaga acgactgggc gacagacgtc acgagcgaag agctcaagac
      181 gatgtgggag ccggcggcag agggcaagat caaccggtgg aaccaggtgc gctccagctg
      241 gcccgacagg ccgttggacc tctacggccc cggtaccgag tcgggcacgt acgatttctt
      301 caacgagacc atcgtcggca acgaagagga agtcaaccgc tcggactacg aggccagcga
      361 ggacgacaac gtgctcgttc agggagtcgc tggggacgtg aacgcactcg ggtacttcgg
      421 ctactcctac tacgagaaca acacagacaa gctcaacgtg ctcgccgttg acggtgtaaa
      481 gcccactccg gactccatcc gctccggcga gtacgttctc tcccgcccgc tgttcatcta
      541 cgtgagcacc gacgcgctga agaagaacaa ggcggtagag ccgttcgtgg acttctacct
      601 ggataacctg gacaagctcg tgcgcgccgc gaagtacgtg acgatgccta acagcctgga
      661 gaaggaggcc tacgcccagt tcgaggaccg caccaccggc acggtgttca acgcggacgg
      721 cgagccgaag ggcggcgacc tggagacggc cctcaagcag tcgcagtaac atgttggtgc
      781 cgggacgggt ctacttgtaa cttggggggt cggcgcagta gctggctccc cactttctct
      841 gctaacgagg tttcggaggg ttttcggtgg cagtcgaagc tggagggacg aggtcccaga
      901 cggaagtctg gaagcgcaat agccgcacca gggaggccag ggagcgggta ataaaggtct
      961 tgctcgcggc ctgcgcgtac ctgtccgtct tcacgacgtt cggaatcgta gtcgtgctct
     1021 tcgaggagac tttgcggttc ttcgtcgagg tctctccggt ggagttcctg acctcgaccc
     1081 ggtgggcgcc gcagcaggac gccttcggca tcctgccgct cgtctacggc acgctcatgg
     1141 cgaccgtcat cgctatagcc gtggcgctcc ctgtggggtt gctcacggcc atctacctca
     1201 gcgagtacgc gccgacgtcg ctcaggcggt ggctcaagcc ggcgctggag atcctggccg
     1261 gcgtgcccac cgtagtctac gggtacttcg ccctgacgtt cattaccccg ttcttcaggg
     1321 agagcgtctt cccttccata ggctcgttca acgcgctctc ggccggccta atcatgggcg
     1381 tgatgatcat cccgacggtg gcctcggtca gcgaggacgc catctacgcg gtaccgcagt
     1441 ccttgaggga gggcgcctac gctctgggcg cgacgaggcg cgagacggcg acgaagatcg
     1501 tggtgccggc tgccctctcc gggatcgttg cggcggtgat cctgggcgtc tcgcgcgcca
     1561 tcggggagac gatgatcgtg accatagcga tgggctccca gccgaacttc ggcgggagtc
     1621 ctctggagag catgcaggct atgacggcgt acatcgtgca ggtggtgggc ggtgagacgc
     1681 cgcgcggctc gatcaccttc aagtccatat tcgcagtggg gtcggcgttg ttcctgataa
     1741 ccctgaccct caacctcgtg agctactggt tcgtccggcg atacagggag atctactagt
     1801 ggctcagagc acatcgcagg tgaagcggcg cggagaatcg gacttcaagg cctccatgtc
     1861 aacgcgctac gggctcgact gggccttcac cgtgctggtt acggtggcgg cgctgatcgg
     1921 gataattgtc ctggtggtgc tggtggtgga cgtgacccgg gacggatcgt cgatgctcag
     1981 cctgaacttc ctgacgagct tcccgtccca gatcttcccc gagaacgggg gcatctaccc
     2041 ggcgctcatc ggctcgctgt ggctgctggg gttgacggcg ctcataagcg tgccgctagg
     2101 gctcggggcc gcggtgtacc tcgaagagta cgcacaggac acgcgtatca acaggatagt
     2161 cgaggtcaac atctcgaacc tcgccggggt gccgtctgtg atctacggtc tgcttggcct
     2221 tggcatcttc gtccagctcc tcgcgccggt caccggcggc acgagcgtgc tcacaggcgc
     2281 gctgacgttg agtttgctga tcctgccggt catcatcgtc gctaccaggg aggcgctcag
     2341 agccatcccc gcctccatcc gcgagggcgg gtacgcgctc ggggcgacga agtgggaggt
     2401 gatccggagc cacctgctcc cgatggcgct gcccggcgcg ctgacgggca taatcctggc
     2461 gctctcgcgg gctatcggcg aggcggcccc gatactggtc gtcggggtct cgttgtacca
     2521 gacttacgtc acaaccggtc cattcgacgg ctacatggcg cttcctactc agatctacga
     2581 ttggatcagc cgcccgcagc aggtcttcca ggactccgcc gcagcaggca tagtggtgat
     2641 catggtggtc ttgctgctgg cgaactcgat cgccatcgtg gtgcgcaaca ggtatcagaa
     2701 gcgtgcttga gtacggttct cgcgaaggaa aggtgctagc ctagtggata ccgagagggc
     2761 caggagggag cgaagggtgt ccgagctttc agaggaacag agcgaggtga ggcagcgagg
     2821 cgaggggact cccgtcggcg acggctccac cctggggatg aaggtccagg atctctccgc
     2881 ctactacggt cccttcaaag ccataaccga cgtgagcatg gacattcagg ccaacaaggt
     2941 cacggcggtg atcgggcctt ctggatgcgg caagagcacc ttcatccgct gcctgaaccg
     3001 catgcacgag gtgatcaccg aggcgcgtgc cgaagggtgc gtgatgctcg gcgacaagga
     3061 catctacgac aggaaagcgg acccggtcgt gatccgtcgg agggtgggga tggtcttcca
     3121 gaagcccaac cccttctcca aatccatcta cgagaatgtc gcgtggggtg ccaggatcaa
     3181 cggcttcagg ggcgacatgg acgagttggt cgagcagtcc ttgaggcacg ccgccctctg
     3241 ggacgaggtc aaggacaagc tgaagaag
//
LOCUS       DASTUV010000016         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_999195, whole genome shotgun sequence.
ACCESSION   DASTUV010000016 DASTUV010000000
VERSION     DASTUV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999195"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFT03_00325"
     CDS             <1..610
                     /locus_tag="VFT03_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU4846614.1"
                     /translation="LALHLPLRRGAWNLSPEKTLVRSARLARGAGIPVVVAHPPPPGR
                     PLERWAAGPLREAREQGVSVTVENMPRSEPRRVFSIGRYRSCYLPEHLLGLGDVTFDT
                     SHVGASKLDLMRAYSTLANQLQHVHLSDSDLTGGDQHRLPGKGRLPLRPFLSALANSD
                     YPGVVSLELKPWPLGAPDPDTILAQMRGALEFARTALRARPR"
     gene            667..1770
                     /locus_tag="VFT03_00330"
     CDS             667..1770
                     /locus_tag="VFT03_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU4846615.1"
                     /translation="MRDERLQKLARVLVDYSVEAAEGEQVLIAGGAPAQPLIKEVYTR
                     VLDAGAIPVPQVALPGLQEIFFEHARDIHYQKTPPALRAVYEGADAVITIMAPHNTKA
                     LANVDPRKQQALGKRDKALQDMILEKDRWALTLFPTQALAQESEMSLEEYEEFVFEAM
                     ALNEDDPVRYWREKAEEQRRLIERLEKASEVRVVGPETDLTLSIEGRRFLNGDGKHNM
                     PCGEIFTGPVEDSANGEIYFGVPSAVAGREVSGVRFRFEDGRVVEASAEKGEEYLYAM
                     LDADEGARYLGELGIGTNFGISRATRNILFDEKLGGTVHLAIGRSYEKTGGKNDSSVH
                     WDLICDLREGGELRADGETVQRDGRFPDYDFAA"
     gene            1780..2028
                     /locus_tag="VFT03_00335"
     CDS             1780..2028
                     /locus_tag="VFT03_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846616.1"
                     /translation="MSGERLGETMLRVANIEDEVALESVRDALDALDMVYEHIRSEPD
                     EDTFPQSAYFYVPDTFADDVDNVMQRLAEEYGFEAEVL"
     gene            2025..>2672
                     /locus_tag="VFT03_00340"
     CDS             2025..>2672
                     /locus_tag="VFT03_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4846617.1"
                     /translation="MTRRGLQVRYDAVFFDVDGTLLWVHLDVEGYVKDLAPYATNGSL
                     TVERAKEPVREGMRRHIEKNIEYRTEEDLAGFKRRNAEITASTLGIRAPTDLLAAVAD
                     RRISFNPYPESEAVLRRLREMGVGMYVVSNWDLGLAGMLEDLGWMRYFDDLVVSAISG
                     VEKPDQRIFEEALEASGVDRRRVVHVGNDPVTDISGAAKVGIDTVLVDRRGNVFAP"
BASE COUNT          495 a          829 c          926 g          422 t
ORIGIN      
        1 cctggctctc catctgccac tgcgccgcgg cgcctggaac ctctctcccg aaaagaccct
       61 ggtccgctcg gcccgtctcg cccgcggtgc cgggataccc gtcgtcgtgg cgcacccgcc
      121 gccaccgggc aggccgctgg agcgctgggc cgctgggcct ctgcgcgagg cgcgcgagca
      181 gggcgtctcg gtcacggtcg agaacatgcc gcgctccgag cctcgccgcg tcttcagcat
      241 cgggcgctac cggagctgct acctgcccga gcacctcctc ggtctgggag acgtcacctt
      301 cgacaccagc cacgtcgggg cgagcaagct ggacctgatg cgcgcgtact ccacgctcgc
      361 gaaccagctc caacacgtcc acctctccga ctcagacctc accggcggcg accagcaccg
      421 cctcccgggc aagggccggc tccccctgag gccgttcctc tccgctctgg cgaacagcga
      481 ctacccgggc gtcgtcagcc tggagctcaa gccctggccc ctcggagccc ctgaccccga
      541 caccatactg gcgcagatgc gcggggcgct ggagttcgcg cgcactgcgc tgcgcgccag
      601 gccgcgctga gaacagatga taagctctcc gaactgttcg catgagcggc tcgagaggcg
      661 gattctatga gagacgagcg tttgcagaaa ctggcgcggg tcctggtcga ctactcggtc
      721 gaggcagcag agggggagca ggtcctgatc gccggcgggg cgccggcaca gcccctcata
      781 aaagaggtct acaccagggt cctcgacgct ggcgccatcc ctgtcccgca ggtcgccctc
      841 ccaggcctcc aggagatctt cttcgagcac gccagagata ttcactacca gaagaccccg
      901 ccagccctgc gcgccgtcta cgaaggcgcg gacgccgtca ttacaatcat ggcgccgcat
      961 aataccaagg ctctcgctaa cgtggacccg cgcaagcagc aggctctggg gaagcgggac
     1021 aaggcgttgc aggacatgat cctggagaaa gacagatggg cgttgacgct ctttccgacg
     1081 caggcgctgg cgcaggagtc cgagatgagc ctcgaggagt acgaggagtt cgtcttcgag
     1141 gcaatggcgc tgaacgagga cgacccggtc cgctactggc gtgagaaggc tgaggagcag
     1201 aggaggttaa tagagcgtct ggagaaggcg agcgaggtga gggtagtggg ccctgagacg
     1261 gacctcacgc tctccatcga ggggcgcagg ttcttgaacg gcgatgggaa gcacaacatg
     1321 ccgtgcggtg agatcttcac agggcctgta gaggactcgg cgaacggtga gatctacttc
     1381 ggcgtcccgt cggccgtcgc ggggcgcgag gtctcggggg tgcggtttcg cttcgaggac
     1441 ggcagggtgg tcgaggcgag cgccgagaag ggggaagagt acctgtacgc catgctcgac
     1501 gcggacgagg gggcgcgcta cctcggggag ctcggcatcg ggacgaactt cggcatctcc
     1561 agggcgacga ggaacatact cttcgacgag aagctcggtg gaacggtgca tctggcgatc
     1621 gggcgttcct acgagaagac agggggcaag aacgactcga gcgtgcactg ggacctcatc
     1681 tgcgacctgc gcgagggcgg cgagctgcgg gccgacggcg agaccgtgca gagagatggc
     1741 cggttcccgg actacgactt cgcggcttga ggaggcaggg tgtcgggaga gaggctggga
     1801 gagacgatgc tccgggtggc gaacatcgag gacgaggtcg cgctcgagag cgtgcgcgac
     1861 gctctggatg cgctcgacat ggtctacgag cacatccgct cggagcccga cgaggacacc
     1921 ttcccgcaga gcgcctactt ctacgtcccc gacaccttcg cggatgacgt ggacaacgtt
     1981 atgcagcggc tcgccgaaga gtacggtttc gaggccgagg ttttgtgacg cgtagggggc
     2041 tccaggtacg ctacgacgca gtctttttcg acgtggacgg tacgctactc tgggtacatc
     2101 tcgatgtgga gggttacgtt aaggacctgg cgccctacgc gacgaacgga tcgcttaccg
     2161 tcgagagggc gaaggagccg gtgcgggagg gtatgcggcg gcacatagag aagaacatcg
     2221 agtaccgcac ggaggaggac ctcgccggct tcaagcgccg aaacgccgag atcaccgcaa
     2281 gcaccctggg cattcgggct ccgacggacc tccttgctgc ggtcgccgat cgcagaatct
     2341 ccttcaatcc ctaccccgag tccgaggctg tgctgcgccg gctcagggag atgggggtgg
     2401 ggatgtacgt cgtctcgaac tgggacctcg ggctagccgg gatgctcgag gatctcggct
     2461 ggatgcgcta cttcgacgac ctcgtcgtct ctgcgatctc cggggtcgag aaaccggacc
     2521 agagaatctt cgaggaggcg ctcgaggcca gcggcgtcga caggcgcagg gtggtccacg
     2581 tcggcaacga tcccgtcacg gacataagtg gggctgcaaa agtgggaatt gacacggtgc
     2641 tcgtggaccg caggggcaac gtctttgctc cc
//
LOCUS       DASTUV010000017         3633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_497903, whole genome shotgun sequence.
ACCESSION   DASTUV010000017 DASTUV010000000
VERSION     DASTUV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497903"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..566
                     /locus_tag="VFT03_00345"
     CDS             255..566
                     /locus_tag="VFT03_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU4846618.1"
                     /translation="MGGTDLGRFSDPALFVLASLAEGEKHGYAMMEDIEEMSGTRLGP
                     GTLYGALARLERRGLVEPLGSAGRRKPYRLTAAGATALEERLRNLEGFARTGLGRLAT
                     T"
     gene            563..1486
                     /locus_tag="VFT03_00350"
     CDS             563..1486
                     /locus_tag="VFT03_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846619.1"
                     /translation="MRRLERLLAGLYPRAWRERYEEEFVAMLEQRPASVSDLVDVAFG
                     VLDAWVRPQVASEGGRLVIPKMRTSLLVVLWAWAGFVAAGVGFQKMSEYEDFVSAARE
                     NLAVGISFETVVVGAVVALAAVVVGGVPIVLAAVRQALAEGRRDVPLLFCVPLFSAAA
                     FVGYVLVLVKVIYPSLGRLAVHDALNVALFVSLTGAFLLAAAASVAAVSVAVRRCEIG
                     ARFYRFALFPGTLTALAMGVVVVATVVWGLALRAQAPALFSGNEGILATPTIASWFVI
                     VAVMAACACAASAAMVRGLRARRGEISQAGQ"
     gene            1592..2989
                     /locus_tag="VFT03_00355"
     CDS             1592..2989
                     /locus_tag="VFT03_00355"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU4846620.1"
                     /translation="MSQHETPHRVERDSMGEVQVPRDALFGAQTRRALDNFPISDLRK
                     PRRFVQALGAIKVEAANVNHELGLLDEDLRDAIVRAAEEVMDGELDNQFVLDVFQTGS
                     GTSTNMNANEVISNRAIQVMGGELGSKEPVHPNDHVNMGQSSNDVIPTAIHLAALISM
                     QRDLLPALEKLQRALEVKAREFDDVIKTGRTHLQDATPIRLGQEFEGYAGQIERGITR
                     VHKAMDELAEVALGGTAVGTGVNTHPEFAQRVTERLSARFGVEVRETGNHFQAQSAMD
                     GTVFASGALKTVAVSLMKIANDIRWLGSGPRAGIGEITLPEVQPGSSIMPGKVNPVIA
                     ESVAMVAAQVMGNDATITIAGQSGNFELNVMLPLIAYNLLQSIELLANAAENLADQCV
                     VGIQATERGPELVEKGLMLATALAPGIGYDRAAEISKEAYKTGRTIREVARERTDLSE
                     EELDNLLDARKMTEA"
     gene            3070..>3633
                     /locus_tag="VFT03_00360"
     CDS             3070..>3633
                     /locus_tag="VFT03_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846621.1"
                     /translation="MADLTSTQVAGVPEPGSAEELRRFAQIQERLAPLAERLVADPRI
                     PQTVVVVPSLTLDLEELAKIPGVHHYEERLLCMLMLLRMPRTHLVFVTSQQVPTAIVD
                     YYLHLLPGVPLRHARGRLTLLSCHDSSDVPLTQKILDRPRLLDRIKGAIADPESAHLS
                     CFNSTPLERSLAVRLGIPLYGNDPELNY"
BASE COUNT          643 a         1154 c         1212 g          624 t
ORIGIN      
        1 cttacggacc acgagcgccg tctccccacg ctctagcgcc ctgacggcga ccgcccactc
       61 cttcaacgtg tgcctcaaag agccgtgttg cggggattcc aaagagtaca cctccgtcaa
      121 gatcgctaaa caacaattat atgaccttac gccgatgggg tccgctaatc ttagaggttg
      181 acaccacaca ccccgagcgt aatactacgt ccggtgtaat attacgtcgt acgtaattgg
      241 tatgggaggt atggatgggt ggcacggatc tggggcggtt ctctgacccg gcgctctttg
      301 tcctcgcgag cctggccgag ggcgagaagc acggctacgc aatgatggag gacatagagg
      361 agatgagcgg caccaggctc gggcccggca ccctctacgg tgcgctggcg cggctggaga
      421 ggcgaggtct ggtagagccg ctgggctcgg cgggcaggcg caagccctac cgtctgacgg
      481 ctgcaggtgc tactgctctg gaggagcggc tgaggaatct cgagggcttc gctcgcacgg
      541 ggctggggag gctggcgacg acgtgaggcg gcttgagagg ttgttggccg gcctctaccc
      601 gcgggcgtgg cgcgagcgct acgaggagga gttcgtagcg atgctcgagc agaggccggc
      661 gtcggtttcc gatctcgtgg acgtggcgtt tggcgtgttg gacgcctggg tgaggccgca
      721 ggtagcgtcg gaaggaggaa ggctcgtgat cccgaagatg cggacttcgt tgctggtggt
      781 gctctgggcc tgggccggct ttgtcgcggc gggcgtgggg ttccagaaga tgagcgagta
      841 cgaggacttc gtgagcgcgg cgcgtgagaa cctggcggtt gggatctcct tcgagacggt
      901 tgtggtcggc gccgtagtcg cgctggcggc ggtcgtggtc gggggggtgc ccatagtcct
      961 agccgcggta cgccaggccc tcgccgaggg gcgcagggac gtaccgctgc tcttctgcgt
     1021 ccccctgttc agcgcggcgg ctttcgtcgg gtacgtgctc gtgctcgtga aggtgatcta
     1081 tccctcactc ggacgtcttg ctgtgcacga cgccctcaac gtcgcgctct tcgtgtcgct
     1141 gacaggcgcg ttcttgctcg ctgcggcggc cagcgtggcg gcggtctcgg tcgcggtcag
     1201 acggtgtgag atcggggcgc gtttctaccg gttcgccctc tttcccggta ctctcaccgc
     1261 cctcgcgatg ggcgtggtgg tggtcgcgac agtcgtctgg gggttagctc tgcgggcgca
     1321 ggcgccggcg ctgttctcgg ggaacgaggg gatcctggcc acccccacca tagcgtcgtg
     1381 gtttgtgatc gtggcggtca tggccgcgtg cgcgtgcgct gcgagcgccg cgatggtacg
     1441 cgggctgcgc gccaggcgag gcgagatctc ccaggctggt cagtaaggcg ccgttgtctc
     1501 actgttaacg gcttcgcggc tggcgatgaa cctccgccgg tagttcgagc ataatatggt
     1561 cctgaacgtt cgagatctga cgggaggaac catgtcccag cacgagaccc cgcaccgcgt
     1621 agagcgcgac tcgatggggg aggtccaggt ccctagagac gccctcttcg gcgcccagac
     1681 gcgccgcgcc ctcgacaact tccccataag cgacctgcgc aagccacgcc gcttcgtgca
     1741 ggcgctgggg gcgatcaagg tcgaagccgc caacgtcaac cacgagctgg ggcttctcga
     1801 cgaagacctc agggacgcca tcgtccgggc tgccgaggag gtcatggacg gcgagctcga
     1861 caaccagttc gtcctggacg tcttccagac agggtcaggg acatccacca acatgaacgc
     1921 caacgaggtg atctcgaacc gggcgatcca ggttatgggt ggcgagctcg gctccaaaga
     1981 gcccgtccac cccaacgacc acgtcaacat gggccagtcc tccaacgacg tcatccccac
     2041 ggcgatacac ctggcagcct tgatctccat gcagcgggac ctcctcccgg ccctcgagaa
     2101 gctacagaga gcactcgaag tgaaggctcg agagttcgac gacgtcataa agacaggcag
     2161 gacgcatctc caggacgcca ccccgatccg tctcggccag gagttcgagg gctatgcggg
     2221 ccagatagag cgcggcatca cgcgcgtcca caaagcaatg gacgagctcg ccgaggtagc
     2281 cctcggcggc acggcggtcg gcaccggcgt caatacccac cccgagttcg cccagagggt
     2341 gaccgagcgc ctgagcgccc gcttcggcgt cgaggtgcgc gagaccggga accacttcca
     2401 ggcgcagagc gcgatggacg gtaccgtctt cgcgagcggc gcgctgaaga cagtcgcggt
     2461 cagcctgatg aagatagcca acgacatccg ctggctcggg agcgggcctc gcgccggcat
     2521 cggggagata acgttgcccg aggtccagcc gggctcctcg atcatgccag gcaaggtcaa
     2581 cccggtcatc gcggagagcg tcgcgatggt agccgcgcag gtcatgggca acgacgccac
     2641 cataacgatc gcgggccaga gcgggaactt cgagctcaac gtcatgctcc cgctcatcgc
     2701 ctacaacctg ctgcagtcta tcgagctcct cgccaacgcc gctgagaacc tcgctgacca
     2761 gtgcgtcgtc ggtattcagg ctaccgaaag aggccccgag ctcgtcgaga agggcctcat
     2821 gcttgcgact gcactcgcac ccgggatcgg ctacgaccgt gcggccgaga tctccaagga
     2881 ggcctacaag acaggccgca ccatccgcga ggtagcccgc gaaaggacag atctctccga
     2941 agaagagctg gacaacctcc tcgacgcccg caagatgaca gaggcttaag cgcgagcatt
     3001 ccaggccacg tgctgaccgc tgggcgctgc gacttggtcc tctgtataat taccggccag
     3061 ggaaaaggga tggctgatct aacttccacg caggtagcgg gtgtgcccga gccgggctct
     3121 gccgaggagc tccggcgctt cgcgcagatt caggagcggc ttgcgccgct ggccgagcgg
     3181 ctggtcgccg acccgcgtat cccgcagacg gtcgtcgtgg tgccgagcct cacgctcgac
     3241 ctggaggagc tggccaagat acccggcgtg caccactacg aggaacgcct cctttgcatg
     3301 ctgatgctgc tgcggatgcc gcgcacccac ctggtcttcg ttacgagcca gcaggtcccg
     3361 acggcgatcg ttgattacta cctgcatctc ctcccaggcg tcccgctgcg ccacgctcgc
     3421 ggaaggctga cgctccttag ctgccacgac tcctcagacg tgcccctgac gcagaagatc
     3481 ctggaccgtc cccgccttct agaccgcata aaaggcgcca tcgcagatcc cgagtcggcg
     3541 cacctgagct gcttcaacag cacgcccctg gagaggagcc tcgcggtgcg gcttggcata
     3601 ccgctgtacg gcaacgatcc cgaactgaac tac
//
LOCUS       DASTUV010000018         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1678585, whole genome shotgun sequence.
ACCESSION   DASTUV010000018 DASTUV010000000
VERSION     DASTUV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678585"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFT03_00365"
     CDS             complement(<1..328)
                     /locus_tag="VFT03_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846622.1"
                     /translation="MYVDFTIRWNVFSFVPHPLSYIFRVLVGLLSSRIAREKDMILKE
                     FSRILVEELDARRLASGDASRPVRGLAVEELAVGWLEKDEVAITSRETLDEGFLKSAA
                     EAGAPAI"
     gene            469..>2891
                     /gene="uvrA"
                     /locus_tag="VFT03_00370"
     CDS             469..>2891
                     /gene="uvrA"
                     /locus_tag="VFT03_00370"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4846623.1"
                     /translation="MPRDVMVVVTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQF
                     LGQMDKPDVDHIDGLSPAVSIDQKTTSNNPRSTVATVTEIYDYLRLLYARAGRPHCHV
                     CGFPVASSTSQQMVEKILELPEGTRFMVLAPVVRGRKGEYGRFLQELAEDGYARVRVD
                     GALHELPVDLNLDKNYKHDIEVVVDRLVIRDGIERRLTDSVETTLRLAEGLVLVETVE
                     RDGSGAEGESMLFSETFTCTNCGASIAEIQPRTFSFNSPHGACDRCDGLGSRLEIDPD
                     LVVPNDDLSVNEGAVVPWANSQTEYHGSVLSALAEKYGIDLDRPWRELPEEHKNTILY
                     GTNGGRIYVSYRNRYGRRRQYMTHFDGVVSNLERRYAETDSEYRRERIEEFMSHVPCP
                     KCKGARLRPEALAVTVGEKNIYEFTELSVLDTQDFFGTVEFTPREWIIAERVVKEIRE
                     RLGFLVDVGLGYLTLSRSAGTLSGGEAQRIRLASQVGSGLVGVLYVLDEPSIGLHQRD
                     NRRLLNTLMRLRDLGNTLIVVEHDEETIRTADYLVDVGPGAGVHGGEIVATGSVYDIE
                     AEERSVTGQFLAGRRRIEPPEERREPTGAVELLGASEHNLKSVDVEFPLGVFTCVTGV
                     SGSGKSTLVNEILYKALANAVSRGKHRPGRHAGLRGVEDVDKVIDIDQSPIGRTPRSN
                     PATYTKVFDHIRQLFASTPEAKIRGYKPGRFSFNVKGGRCEACRGDGQIRIEMHFLPD
                     VYVPCEVCKGRRYNNETLRVTYKGKSISDVLDMTVDEACEFFAPVPAISRRMDTLRDV
                     GLGYIRLGQP"
BASE COUNT          572 a          891 c          959 g          469 t
ORIGIN      
        1 cgatggcagg cgcaccggct tccgcggcgc ttttcaagaa gccttcgtcg agcgtctccc
       61 gggacgtgat ggcgacctcg tccttctcga gccagccaac ggctagctct tcgaccgcga
      121 ggccgcggac gggccgggaa gcgtcgccgg acgccaggcg acgggcgtcc agttcttcca
      181 ccagtatccg ggaaaactcc tttaggatca tgtccttttc tctcgcgatg cggctcgaca
      241 gaaggccaac aagcacccta aatatatacg agagtgggtg tggaacgaaa gaaaagacgt
      301 tccagcgaat ggtaaaatcc acgtacaccc gaatagaccc tttggccctc taataggggg
      361 ccgtatactt gcccaaactc gagagcgaag gggagaacca agatcgccga gagtcagata
      421 gtcgtccgcg gggcccgcga gcacaacctg aagaacataa cagtctcgct gccgcgggac
      481 gtcatggtcg tcgtgaccgg gctctccggg agcggcaagt cctcgctcgc cttcgacacc
      541 atctacgccg agggccagag gcgctacgtc gagagccttt cggcctacgc caggcagttc
      601 ctcgggcaga tggacaagcc ggacgtggac cacatagacg gcctgtcgcc cgcggtctcc
      661 atagaccaga agacgacgag caacaacccc cgctcgaccg tcgcgaccgt aacggagatc
      721 tacgattatt tgaggctcct ttacgcgcgc gcggggcggc cccactgcca cgtctgcggc
      781 ttccctgtag cttcctccac gtcgcagcag atggtcgaga agatcctcga gcttccggag
      841 gggacgcgct tcatggtgct cgcgccggtc gtgcggggga ggaagggcga gtacgggcgg
      901 ttcttgcagg agctggccga ggacggctac gcgcgggtgc gggtggacgg agcgctgcac
      961 gagttgcccg tagacctcaa cctggacaag aactacaagc acgacatcga ggtcgtggtg
     1021 gacaggctcg tgatccggga cggcatcgag cgccgtttga cggactccgt cgagaccact
     1081 ctgaggttgg cagagggcct cgtcctcgtc gagacggtcg agagggacgg tagtggagcc
     1141 gagggagagt cgatgctctt ctcggagacg ttcacgtgca ccaactgcgg tgcctcgatc
     1201 gccgagatac agccgcgcac cttctcgttc aacagcccgc acggggcgtg cgaccgttgc
     1261 gacgggctcg ggagccggct ggagatagac cccgacctcg tagtgccgaa cgatgacctg
     1321 agcgtcaacg aaggcgccgt cgtgccgtgg gccaactcgc agaccgagta ccacggcagc
     1381 gtcctctcag cgctcgccga gaagtacggg atagacctcg accggccgtg gcgcgagctg
     1441 cccgaagagc acaaaaacac tatcctctac gggaccaatg gcgggcgtat ctacgtctcc
     1501 taccgcaacc gctacgggag aaggcgccag tacatgaccc acttcgacgg ggtcgtctct
     1561 aacctggagc gccgctacgc cgagactgac tcggagtacc ggcgggagag gatagaggag
     1621 ttcatgagcc acgtcccctg cccgaagtgc aagggagcgc gcctcaggcc cgaggcgctc
     1681 gccgtgacgg tgggggagaa gaacatctac gagttcaccg agctcagcgt cttggatacg
     1741 caggacttct tcggcactgt cgagttcacg ccgcgggagt ggatcatcgc cgagcgtgtc
     1801 gtcaaggaga tccgcgagcg ccttggcttc ctcgtggacg tgggcctcgg ataccttact
     1861 ttgagtcgct ccgccggtac tctgtctggt ggtgaagccc agcgcatccg gctcgcctcc
     1921 caggtcggca gcggtctcgt tggcgttctc tacgtcctcg acgagccgtc catcgggttg
     1981 caccagcggg acaatcggag attgctcaac accctcatga ggctgaggga cctcgggaac
     2041 acgctcatcg tcgtcgagca cgacgaggag acgatccgga ccgccgacta ccttgtggac
     2101 gtgggaccag gggcgggggt gcatggaggc gagatcgtgg ctaccggtag cgtgtacgac
     2161 atcgaggcgg aggagcgctc cgtgaccggc cagtttctgg caggccgccg caggatagag
     2221 ccgcccgagg agcgtcgcga gccgaccggc gccgtcgagc tgctcggggc gagcgagcac
     2281 aacctcaaga gcgtggacgt ggagttcccc ctcggggtgt tcacgtgcgt gacaggggta
     2341 tccgggtcgg gcaagagcac gctggtgaac gagatcctct acaaggccct cgccaacgcg
     2401 gtgagccgcg gcaagcaccg tccggggagg cacgcggggc tcagaggggt cgaggacgtg
     2461 gacaaagtca tagacataga ccagagcccc ataggaagga cgccgaggtc gaacccggcg
     2521 acctacacca aggtcttcga ccacataagg caactcttcg cttccacgcc ggaggccaag
     2581 atccggggct acaagcctgg ccgcttcagc ttcaacgtca agggcgggcg ttgcgaggcg
     2641 tgccgggggg acggtcagat ccgtatagag atgcacttcc tcccagacgt ctacgtgcct
     2701 tgtgaggtct gcaagggccg ccgctacaac aacgagaccc tgcgcgtcac ctacaagggc
     2761 aagagcatct cggacgtcct cgacatgacg gtggatgagg cgtgcgagtt cttcgccccg
     2821 gtgccggcga tctcgcgccg catggacact ttgagggacg tagggctggg gtacatccgg
     2881 ctcggccaac c
//
LOCUS       DASTUV010000019         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_784061, whole genome shotgun sequence.
ACCESSION   DASTUV010000019 DASTUV010000000
VERSION     DASTUV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784061"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1086
                     /locus_tag="VFT03_00375"
     CDS             220..1086
                     /locus_tag="VFT03_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU4846624.1"
                     /translation="MRLATVRLEGVETAAVVLDNGAVPMVVINDSLGTGWPTELLLLI
                     AGDHLGDLRRWRDELSADELEEISDEAVPLSEISYAPLYRRPRKIWGIGLNYVEHAED
                     LEEASPTDEPASFMRPDTTIIGPGEEIVLPTQSERVTAEAELALIIGREAKDVSEEDA
                     ASVVAGFTTVLDMTAEDILRKNPRYLTRSKSFDTFFSFGPELVTPDEIADLDALEVST
                     VLNGEVRRSNTVSNMMFSPWWLVSFHSQVMTLLPGDIISTGTPGAVVIREGDVVECRI
                     AGFEPLSNPVVR"
     gene            1452..1805
                     /locus_tag="VFT03_00380"
     CDS             1452..1805
                     /locus_tag="VFT03_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846625.1"
                     /translation="MRLALGNIGIGARSRTGGLGETPVRYWDEDDRRARYNAVSDALW
                     IYQDREVEQNLRRFGWRQARRRVLEHGPLSREWINRGRGELRLTRGGLQMRFARGVRT
                     IGWRELKDRIEARRS"
     gene            2075..2863
                     /locus_tag="VFT03_00385"
     CDS             2075..2863
                     /locus_tag="VFT03_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4846626.1"
                     /translation="MDLGLEGKVALVMASSKGLGSAVATQLAREGARVVISSRDEETL
                     SRTATEIAEETGAEVEPRAADITRAEAVKALFSHTTERFGGLDVLVNNTGGPPAGTFD
                     DFDDEGWQAGFELVLLSLIRAVREALPLMRERGGGRIVNIASSSIKQPIENLILSNTF
                     RAAISGLAKSLAIELAPDGILVNTIGPGRISTARSRSMDARNAEAAGVAVEEVRGRFE
                     AQIPLGRYGTPEEFARVVAFLASPANSYVTGQSILVDGGMVRAL"
     gene            2890..>3856
                     /locus_tag="VFT03_00390"
     CDS             2890..>3856
                     /locus_tag="VFT03_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU4846627.1"
                     /translation="MLVPKNSVGVWAFGPAVTRFVPPGYHPEVAGEPMVDKTRRVAEG
                     LSDLLDGLEYHYPGEVNEENAEEILQVLREHGMDLPVVAAGLHTDPTYALGAFVNPDD
                     RLRRQGIDTLKRGVDLCAQVGASFIIWPGAEGYNYTFQRSYAHVWRGFVEGIAEVTDH
                     ANGRGVKVFLEHKNSEPAMKILMKNIGMTLFTIQKVNALGVDTSNLLVNMDWQHLIMN
                     GESLAEYADLLAAESKLGHQHGNDGWGTFDDDNVVGTNFFMQTLELAQTLQDVGYGEN
                     EELIGFDLYPYTEDQVAAVRRAILQWEFIWDLAHKIDRGSLREAKS"
BASE COUNT          778 a         1182 c         1264 g          632 t
ORIGIN      
        1 cacaatttgc gcccggtccg tgagcacccc ggcgtgtaac cgcaagacta tcgccacctc
       61 gatttccggc tcctccccgc cgtgaccgag aagcggatct ttggagcgtt ctaggtctga
      121 ctattacacg gtactgcaac ttccaacaac gcttctactg cgtcagaata cttacggcgg
      181 atgccagcaa atcaggcccc gcgacgaaag gagacaaggt tgaggctggc gacggtgagg
      241 ctcgaagggg tggagactgc ggccgtcgtg ctcgacaatg gtgcggtacc gatggtcgta
      301 ataaacgact cactcggcac ggggtggccc acagaactcc tgctcctcat cgccggagat
      361 cacctcggag atctccggcg ctggcgagac gagctctcgg cggacgaact ggaggagatc
      421 tcggatgaag cggttcccct ctccgagatc tcgtacgcgc cgctctatcg ccgaccgcgc
      481 aagatctggg gcatcggcct caactacgtc gagcacgcag aggatctgga ggaagcctcg
      541 ccgacggacg agccggcgag cttcatgcgg cccgacacca ccatcatcgg gccgggggaa
      601 gagatagtgc tccccaccca atctgagcgc gtgacagccg aggccgagct ggcactcatc
      661 atcggtcgcg aggcgaagga tgtatcggaa gaagacgctg cttctgtcgt cgcaggcttc
      721 accacggtgc tggacatgac agccgaggac atactacgaa agaaccctcg ctacctcacg
      781 cgctcgaaga gcttcgacac cttcttctcc ttcggcccgg aactcgtgac gcccgacgag
      841 atagcggact tagacgcctt ggaggtctct acggtcttga atggcgaggt ccggcgctcc
      901 aacacggttt cgaacatgat gttctctccg tggtggctcg tctcgttcca ctcgcaggtc
      961 atgaccctgt tgccgggcga cataatctcg acggggacgc cgggggcggt ggtcatacgg
     1021 gagggcgacg tggtggagtg ccgcatagcc ggcttcgagc cgctctccaa ccccgtcgtc
     1081 cggtagagcg gaccacgata atgtggctaa ggggccacat cgagggtgag aggatcgcgg
     1141 cacagttgcc tgagaaaggc tgggaccaac ccgacgccca cacttccaag tagagcacgc
     1201 ctacaggcgg ctcattgatg agcgagtcac gagcagctgc gcgaaaagta ggagacgctc
     1261 caggctctat ctcggtgcag tgccagatga tgtgcgctac gcagtcgtca gcaacgacac
     1321 cgctagaagc gaagaagctc gggaggatcc gttcttatga atcacctaat ccttttctca
     1381 tgaatttcac atcttcgccg gcgagaatcg cgctcgggaa gacaaaatca cgaccagagg
     1441 agtagaaaga gatgaggctg gcacttggga atatcgggat cggcgcgagg agtcggaccg
     1501 gcggtctcgg tgagacacca gtgaggtact gggacgagga tgaccgacgg gcgcgctaca
     1561 acgcagtgtc ggatgcgttg tggatctacc aggatcgcga agtcgaacaa aacctccggc
     1621 gcttcggctg gcgccaggcc cgcagaaggg tgctggagca tggaccgctg agccgggagt
     1681 ggataaaccg cgggcgcgga gagctccgcc tgacgcgggg cggactccag atgcgcttcg
     1741 cgaggggcgt tcggaccata ggctggaggg agcttaagga caggatcgaa gcgcggagat
     1801 cgtaaggctg ccaaccccgg gccgaaggac aaggcgccgg tcgggctcca ccttgcgccg
     1861 cgtcggacgc tgcttatctc cacggctcgc ctgcgccttg atgggcaccc tcgctggcga
     1921 gaacgctggc ctcgcggacg cggcgccacg ccatcggaca gaacgacgcc cgcttcttct
     1981 ggatttcgag caggacatcg ccgagttgcc gccgaccggg ggctcctata acatcttctc
     2041 cgacccgccg tcggaccctg caaggaggta gaggatggat ctcggtcttg agggcaaggt
     2101 cgcgctggtg atggcgtcga gcaagggcct tgggagtgct gtagcaacgc agctcgcccg
     2161 cgagggtgcg agagtcgtga tctccagccg cgacgaagag accctctccc ggaccgccac
     2221 cgagatagcg gaggagacgg gcgccgaggt ggagccccgc gccgccgaca tcacccgggc
     2281 ggaggcggtg aaggccctct tctcgcatac tacggagcgt ttcggaggcc tggacgtgct
     2341 cgtgaacaac accggcggcc cgcccgccgg caccttcgac gacttcgacg atgaaggctg
     2401 gcaggcaggg ttcgagctcg tcttgctgag cctcatccgc gcggtccgcg aggcgctacc
     2461 cctgatgcgc gagcgcggcg gcgggcgcat cgtcaacatc gcgtcatcgt ccatcaagca
     2521 gcccatcgag aacctcatcc tctcaaatac cttccgcgca gctatatcag ggcttgccaa
     2581 gagcctggcc atcgagctcg cccctgacgg tatcctggta aacaccatag gcccggggcg
     2641 catctccacc gcgcgttctc ggtctatgga cgcgaggaac gcggaggccg ctggcgtcgc
     2701 ggtcgaagag gtcagggggc ggttcgaagc gcagatacct ctggggcgct acgggactcc
     2761 ggaggagttc gccagggtgg tggcgttcct ggcctccccg gccaacagct acgtgacggg
     2821 acagtcgatc ctggtggacg gcggcatggt gcgcgcgctg tagcgcgatc gcagggaagg
     2881 agtcgcagga tgctggtgcc gaagaacagc gtgggcgtct gggcgttcgg gccagcggtc
     2941 acccgcttcg tgccgccagg ctaccaccca gaagttgccg gcgagccgat ggtagacaag
     3001 accaggcgcg tggccgaggg gctctctgat cttctggatg gactcgagta ccactacccg
     3061 ggtgaggtga acgaggagaa cgccgaggag atcctccagg ttttgagaga gcacgggatg
     3121 gacctgcccg tggtcgccgc cgggctccac accgacccga catacgcctt gggcgccttc
     3181 gtcaacccgg acgataggct gcgcagacag ggcatcgaca ccctcaagcg cggcgtggac
     3241 ctgtgcgctc aggtaggagc gagcttcatc atctggccag gggcggaagg gtacaactac
     3301 accttccagc ggtcctacgc gcacgtgtgg cgcggcttcg tcgaggggat agccgaggtg
     3361 accgaccacg ccaacgggcg gggcgtcaag gtcttcctcg agcacaagaa cagcgagccc
     3421 gcgatgaaga tcttgatgaa gaacatcggg atgacgctct tcaccatcca gaaggtaaac
     3481 gctcttggcg ttgacacctc caacctcctc gtcaacatgg actggcagca cctgatcatg
     3541 aacggcgaat ctctagccga gtacgccgac ctcctcgccg cggagagcaa gctcggccac
     3601 cagcacggca acgacggctg gggaaccttc gacgacgaca atgtggtggg cacgaacttc
     3661 tttatgcaga ccctcgagct agcccagacg ctccaggacg tcggctacgg ggagaacgag
     3721 gagctaatag gcttcgacct ctacccctac accgaggacc aggtcgcagc ggtcaggcgc
     3781 gccatcttgc agtgggagtt catctgggac ctggcgcaca agatagaccg cggctctctg
     3841 cgcgaggcga agagcc
//
LOCUS       DASTUV010000020         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_963204, whole genome shotgun sequence.
ACCESSION   DASTUV010000020 DASTUV010000000
VERSION     DASTUV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963204"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFT03_00395"
     CDS             complement(<1..334)
                     /locus_tag="VFT03_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846628.1"
                     /translation="MLSPADVEAFISRLEERVAPVEKAVSEAWWRLATTGTEEAQQEL
                     VRAGMEYNNLFADSDEYQEIRGYYQERNSLESSLLRRQVEVLYRTFAGRQGDRETLDR
                     IEELEAKAS"
     gene            complement(342..1136)
                     /locus_tag="VFT03_00400"
     CDS             complement(342..1136)
                     /locus_tag="VFT03_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU4846629.1"
                     /translation="MADQVNYYRVLGVSREASQEEIRGAYRRLAKERHPDHPSGSAEE
                     FSLLQEAYAVLSDPNRRRTHDEALDLAHAADQLAGLDFGSLEDELAARRQERESSGPP
                     LGERLRGRFRRKEKPGSQRGREDVRHRGRYEVREARWYEPHNFDPEPLTWKSGAISFV
                     VAFAAFIAAGQIGLWATAVIDAGPFAFTHVLGPFMPLVYTLVGLVTAYFAYRAAGYAA
                     LALVFVAALAVGSQGLPEGLLQFGTLGIVLLLVAIWLGSRRERAAR"
     gene            complement(1149..2315)
                     /gene="queG"
                     /locus_tag="VFT03_00405"
     CDS             complement(1149..2315)
                     /gene="queG"
                     /locus_tag="VFT03_00405"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU4846630.1"
                     /translation="MNGPRIDPGEAKRILEERARAAGFDLVGVTRAEPLTQGGERLRE
                     WQEAGMAADMGYMQRPVELLSDPKKLQKSAKSVVSLGVSYYPGDHPENTEGGGRVARY
                     AWGRDYHEVIKERLFRLRAELEDELGVKIKARGFTDAVPLLERSAAQHAGLGFFGRNS
                     CLISGEVGSYFFIADLIVDLDLAPDPSGSGTCGRCTRCMDRCPTGAIKAPGVVDARLC
                     ISYLTIENKGEIPRHLRPLVGDWAFGCDVCQEVCPYNKTKAARSRWPEFSEEAGTGPY
                     LEIEEVLGIRSEEEFERSFAGTPLTRPGRAGLLRNCCVAAGNLGLRKAAPALIRCLRE
                     DESPLVRAHAAWALGEIGGAEAELEEAAEGESDPACLEEIKLALAGGTTGASLG"
     gene            2644..>3077
                     /locus_tag="VFT03_00410"
     CDS             2644..>3077
                     /locus_tag="VFT03_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846631.1"
                     /translation="MRFNPIPSNPAKIKQDGITPLDSEQARALLDAGKEDRLEALYVL
                     SLTVGLRIGEALGLKWSDIDLYTGTLCVNRQLQWYTGKGLVFSEPEHGSRRTVDLPQR
                     TSPQKPSQAAGREEGGSVGRKPADAHRVSAVAQAIRRRSRTW"
BASE COUNT          504 a         1056 c          987 g          530 t
ORIGIN      
        1 cgctcgcctt tgcctcgagc tcctcgatgc ggtccagggt ctctctgtct ccctgtcgcc
       61 cggcgaacgt ccggtacaac acctcgacct gccgccgcag gaggctactc tcgagagagt
      121 tccgctcctg gtagtagccc cggatctcct gatactcgtc gctatcggcg aagaggttgt
      181 tgtactccat ccccgcgcgg acgagctcct gctgggcctc ctccgtgccg gtcgtcgcga
      241 ggcgccacca ggcctcgctc acagctttct cgacgggagc gacgcgctct tcgagccggg
      301 agatgaacgc ctctacgtcg gcaggagaaa gcaaggcgcg ctcagcgggc ggcgcgttcg
      361 cggcggctgc ccagccagat cgcgacgagc aggaggacga tgcccagcgt gccgaactgc
      421 agcagcccct ccggcaagcc ctggctgccg acggcgagcg cggctacgaa gacgagcgcc
      481 agcgccgcgt atcccgccgc cctgtaggcg aagtacgccg tcacgaggcc caccagggtg
      541 tagacgagcg gcatgaacgg gccgagcacg tgggtgaagg cgaaggggcc tgcatctatg
      601 actgccgtag cccacagccc gatctggcca gccgcgatga aagcggcgaa ggcgacgacg
      661 aagctgatgg cgccggactt ccaggtcagc ggctccgggt cgaagttgtg cggctcgtac
      721 cagcgagcct cacgcacctc gtagcgtccc ctgtgcctaa cgtcttctct gcctcgttgg
      781 ctaccgggct tctcttttct cctgaagcgg ccgcgcaggc gctcgcccag aggcgggccg
      841 ctcgactcgc gctcctggcg ccgggctgcg agctcgtcct ccagggagcc gaagtcgagg
      901 ccggctagct ggtcggcggc gtgggcgagg tcgagggcct cgtcgtgcgt cctgcggcgg
      961 ttcggatcgg agagcacggc gtaggcttct tgcaggagcg agaactcttc ggcgctgccg
     1021 ctcgggtggt cggggtggcg ctccttcgcc agcctgcggt acgcgcctct gatctcctct
     1081 tgcgacgcct cgcgggagac gccgagcacc cggtagtagt tgacctgatc agccatcgtc
     1141 ttcttattct aacccaacga tgcgccggtg gtcccacccg cgagtgccag ctttatctcc
     1201 tcgaggcagg cgggatccga ctccccctcc gcggcctcct cgagctccgc ctccgcgccc
     1261 ccgatctccc ccagcgccca cgccgcgtga gcgcgcacca gcggtgattc gtcctcccgc
     1321 aggcaccgga tcagggcagg agcggccttc cgaagcccca ggttccccgc cgccacgcag
     1381 caattgcgca ggagcccggc acggccgggc cgggtgagag gcgtgccggc gaagctgcgc
     1441 tcgaactcct cttcggaccg gatgccgagc acctcctcta tctccaggta tggtcctgtc
     1501 cctgcctcct ccgagaactc cggccagcgg ctcctcgcgg ccttcgtctt gttgtagggg
     1561 cagacctcct ggcagacgtc gcagccgaag gcccagtcgc caacgagcgg gcgcaggtgg
     1621 cgcggtatct cgcctttgtt ctcgatggtg aggtaggaga tgcacagccg ggcgtcaacc
     1681 acgccggggg cctttatggc gccggtcggg cagcggtcca tgcagcgagt gcaccggccg
     1741 caggtccccg agccggacgg gtcgggcgcg agatccaggt ccactatcag gtcggcgatg
     1801 aagaagtagg atcccacctc gccactgatc agacacgagt tccgtccgaa gaacccgagg
     1861 cccgcgtgct gggccgccga gcgctcgagc agcgggacgg cgtcggtgaa gccgcgagcc
     1921 ttgatcttca cccccaactc atcctccagc tccgcgcgca gcctgaagag ccgctccttt
     1981 atgacctcgt ggtagtccct gccccacgcg taccgcgcga ccctgccccc gccctcggta
     2041 ttctcgggat ggtcccccgg atagtacgag acgcctagag agacaacgct cttcgcgctc
     2101 ttctgcaact ttttcgggtc cgaaaggagc tcgacgggac gttgcatgta gcccatgtcg
     2161 gcggccatgc cggcttcctg ccactcccgc agccgctcgc cgccctgcgt gagcggctcc
     2221 gctcgcgtca ccccgaccag gtcgaagccc gccgcgcgcg cccgctcctc caaaatacgc
     2281 ttcgcctcgc cgggatctat gcgcggtccg ttcatggcca acgccgatta aatcagacga
     2341 aagacgagcg tgtcggcgat gcgtttcgag ccgctcacct accgcgtcta cggggttagt
     2401 ctggcccttc gcttggcttc ggggctgagc cgggacgcaa ccacagcgag gcgtacgacg
     2461 gctgcatctt ctccctcact acccccaccg gggtgccgtt cagcgtatcc aggtccgtct
     2521 cgtcccgcag cgtcgttgag aacgtttcga gcgtccttcg ggcgtcgtat ttgctgcggt
     2581 agaagcgcct gtcggtaaac gactggatgc ggtgtagctc agtcgcaggg gttagatcag
     2641 gccgtgaggt tcaacccgat accgtcgaac ccggcaaaga tcaagcagga cgggatcacg
     2701 ccgcttgact ccgaacaggc gcgggcgctt ctggacgccg gtaaagaaga tcggcttgaa
     2761 gctctttacg tcctctctct caccgtcggg ttgcggatcg gagaagcgct cgggttgaag
     2821 tggagcgata tagacctcta cactgggacg ctctgcgtca accgtcagct acaatggtac
     2881 accggtaagg gactcgtgtt ctccgagcct gagcacggta gtcgaaggac cgtagacttg
     2941 cctcagagga ccagccctca gaagccatcg caagcggcag gtagagagga gggaggaagc
     3001 gtcggtcgca agccagcaga cgcccacaga gtgtccgcag tggcgcaggc tattcgaagg
     3061 aggtcaagaa catggca
//
LOCUS       DASTUV010000021         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1499051, whole genome shotgun sequence.
ACCESSION   DASTUV010000021 DASTUV010000000
VERSION     DASTUV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499051"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VFT03_00415"
     CDS             <1..1833
                     /locus_tag="VFT03_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4846632.1"
                     /translation="GTNAFDSVHPDDRERASGIFAEILKTPGSHLPLEFRVQHRDGSW
                     RYLEHTVNNLLDDPAMGAVVINSRDITERKRAEAEIRRLNEDLEARVAERTIQLESAL
                     AELEQRERSLLESERQLRQLFENSADALFVHDEHGRFVDCNAQACRALGYTREELLEL
                     SVADLATHLISEEERREKKGRTLWERARRGAPGRMVGFEENELRRKDGTTFPVEVGVG
                     ALEYKGRRLIFASVRDTTERKLAEAALKESERRYRAVVEQAAEGILLVDVDTKRVLET
                     NAAYQDLLGYSTEEALRLSLYELVPYSREDMDCYVERVLESGSYVSGQRRHLRKDGSL
                     VDVEVSANVVYYGGREIICMVVRDITERKRAEEDLRRLNEELEGRVRWRTAQLEAFNN
                     ELEAFSYSVSHDLRAPLRAIDGFSQILLEDYEDKLDEEGRLYLRRTKNASQRMGHLID
                     DLLNLSRMTRSEMRRESVDLSALVEGVVEDLRATSPEHEVDVVVEEGLVVNGDESLLR
                     VALENLLGNAWKFTRDRARPRIEFGTLEHEDTPAYFVRDNGVGFDMAYADKLFGAFQR
                     LHSTGEFEGTGIGLATVQRIIHRHGGRVWAEGNIGSGATFFFTL"
     gene            complement(1970..3208)
                     /gene="hutI"
                     /locus_tag="VFT03_00420"
     CDS             complement(1970..3208)
                     /gene="hutI"
                     /locus_tag="VFT03_00420"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU4846633.1"
                     /translation="MAEVSLLVHDLEAAVSPKGSGPLRDRSLGEFEVHAPASVAISGD
                     RIAAIGSPKEVLRELSFSPDYETVDGRGKVALPGLIDCHAHAAFLGDRVKEFELRSRG
                     ASYEELHAAGGGILSTVSATRAGSEEELSSAVERHLGWMLDHGTLTAEAKSGYGLDRD
                     TELKSLRAIRSADEVHPVDVHPTFLGAHTVPPEFSGAAEYVDFVIRAVLPDAAPLARA
                     ADVFLERGSFEVPEARMYLEACAGYGLALRLHADQFSERGAIPLAIELGARSVDHLEN
                     TGEAGARLLAKSDVAAVLLPACGLFLNLPLPPARLLVEEGAIVALATDFNPGSSFCES
                     LPLVMNLACTRLKLSPAEALCACTANAAHVLGLDDVGRLAPGYKADMLVLDAPDWRYV
                     AYHLGGDRFAARIKAGQVLA"
BASE COUNT          645 a         1036 c         1124 g          529 t
ORIGIN      
        1 gggaccaacg catttgactc cgtccatcct gatgacaggg agcgtgcgtc gggcatattc
       61 gccgagatcc tgaaaactcc tggcagtcac ctacccctgg agttccgggt gcagcacagg
      121 gacggctcct ggcgctacct cgagcacacc gtcaacaatt tgcttgacga cccggctatg
      181 ggggccgtgg taatcaactc gcgagacata accgagcgca aacgagccga ggctgagatc
      241 cggcgtctca acgaggacct cgaagcgagg gtcgccgagc gaaccatcca gttggagtcc
      301 gccctcgcag agttggagca gcgagagcga agtttgctcg agagtgagcg gcaattgagg
      361 cagctcttcg agaactccgc cgatgctctc ttcgtccacg acgagcacgg tcgctttgta
      421 gactgcaatg cccaggcgtg ccgcgcgctg ggatataccc gcgaagagct gctcgagctc
      481 tctgttgcag acctggcgac gcacctgatc tcggaggaag agcgtcggga gaagaaaggc
      541 cggacgcttt gggagcgagc caggcggggc gcaccgggca gaatggtagg cttcgaggag
      601 aacgaactgc ggcgcaagga cggcaccacc ttcccggtcg aggtgggcgt cggagccctc
      661 gagtacaagg ggcggcgcct gatctttgcc tcggtccgcg acaccaccga gcgcaagcta
      721 gctgaggcgg ctctcaaaga gagcgaaagg cgctaccggg cggtggtcga gcaagccgcc
      781 gaaggtatcc tgctcgtaga cgtcgacacc aaacgcgtgc tggagaccaa cgccgcctac
      841 caggatctgc tcggctattc caccgaggag gccttgcgcc tctcgctcta cgagctcgtc
      901 ccctactcca gggaggatat ggactgctac gtagagcgtg tgctggagtc gggcagttac
      961 gtcagcggcc agaggcgcca ccttcgcaag gatggctccc tggtcgacgt ggaggtcagc
     1021 gcgaacgtgg tctactacgg cggtcgggag atcatctgca tggtggtccg cgacatcacc
     1081 gagcgcaagc gcgccgagga agatctccgc cggctcaacg aagagttgga gggccgtgtc
     1141 cggtggcgca ccgcccagct cgaggccttc aacaatgagc tggaggcctt cagctactcg
     1201 gtttctcacg acctgcgcgc ccccttgagg gccatcgacg gcttcagcca gatattgctg
     1261 gaagactacg aagacaagct ggacgaagaa ggcaggcttt acctgaggcg caccaagaac
     1321 gccagccagc ggatgggaca tctcatcgac gacctgctga atctctcccg catgacacgt
     1381 agcgagatgc gccgggaaag tgtggacctc agcgccctgg tcgaaggggt ggtcgaggac
     1441 ttgagggcaa cttcaccgga acacgaagtg gacgtcgtcg tagaggaagg gctggtggtc
     1501 aacggggacg agagcttgct gagggtggcg ctggagaacc tcctgggcaa cgcgtggaag
     1561 tttaccaggg accgggcgcg cccgaggata gagttcggga cactagaaca cgaggacacg
     1621 ccagcgtact tcgtgcgtga taacggtgtg ggtttcgaca tggcctacgc ggacaagctg
     1681 tttggagctt tccagcgctt gcacagcact ggtgaattcg aaggcacagg catcgggttg
     1741 gcgacggtac agcgcatcat ccaccggcac ggtggccgag tgtgggccga gggcaacatc
     1801 ggcagcggtg ccactttctt ctttactctg tagcgaaact agccaccgat tctcgaagta
     1861 atcgatctcg ccaagaactc gggagagact gcgcctccca aaaacaagcg agctacaagc
     1921 ctgacagtcc cgcgtgtaca gagacacgcc gtgagtgtgg accggcagtc tacgccagca
     1981 cctggccggc cttgatcctg gccgcgaagc gatccccgcc gagatggtac gcgacgtagc
     2041 gccagtcggg ggcgtcgagg acgagcatgt cggctttgta gcccggcgcc aatcgaccga
     2101 cgtcatcgag accgagcacg tgcgcggcgt tcgccgtgca cgcacagaga gcttcggcgg
     2161 gggagagctt gaggcgggta caggccaggt tcatgaccag aggcagcgac tcgcagaagg
     2221 agctcccggg attgaagtcg gtggcgaggg cgacgatggc cccctcctcg acgagcagac
     2281 gtgcgggggg gagtggtagg ttcaggaata gcccgcacgc cggcagcagc accgccgcga
     2341 cgtcgctttt ggcgaggagg cgcgcgcctg cttcgccagt gttctccagg tggtccacgc
     2401 tgcgggcacc gagctctatg gcgagcggta tcgccccacg ctcggagaac tggtctgcgt
     2461 gcagcctgag cgccaggcca tagcccgcgc aggcctcgag atacatcctc gcctcaggga
     2521 cctcgaagga gccgcgttcc agaaagacgt cggcggcccg ggcgagcggg gcggcatcag
     2581 ggaggacggc ccggatcacg aagtccacgt actcggccgc gccggagaac tccggcggca
     2641 cggtgtgcgc cccgaggaaa gtcggatgta cgtccacggg atgaacctca tccgcgctcc
     2701 tgatggcgcg cagggacttc agctccgtat cgcggtcgag gccgtagccg ctcttggcct
     2761 cggcggtcag agtgccgtga tcgagcatcc agcccaggtg cctctcgacg gccgagctca
     2821 gctcctcctc gctccccgct ctggtggccg agacggtcga gaggatgccc ccgccggccg
     2881 cgtgcagctc ctcgtagctc gccccgcgcg agcgaagctc gaactccttt accctatccc
     2941 cgaggaaagc ggcgtgagcg tggcagtcta tgagtccggg gagcgcgacc ttcccgcgcc
     3001 cgtccacggt ctcgtagtca gggctgaacg aaagctcgcg caacacctct ttcggggaac
     3061 cgatggcggc tatgcggtcg ccggagatcg cgaccgaggc gggggcgtgg acctcgaact
     3121 cgccaagact ccggtcacgc agcggacctg agcctttcgg ggagacggcg gcctcgaggt
     3181 cgtggactag tagcgagacc tcagccaccc tcttcctccc tggcgtggac gcggcggcct
     3241 cctgaccacg tcgccgccac gaccccgctc cccgccccga agaccagggc ggaggggaga
     3301 tcttccacgt ccgcgacctc cgggtgctcg aggt
//
LOCUS       DASTUV010000022         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1034134, whole genome shotgun sequence.
ACCESSION   DASTUV010000022 DASTUV010000000
VERSION     DASTUV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034134"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..366)
                     /locus_tag="VFT03_00425"
     CDS             complement(94..366)
                     /locus_tag="VFT03_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846634.1"
                     /translation="MDRSVLRADRLRAALDSAVVGLLLFSVVVSTIAGAGAVLYGTVL
                     TAWMLFPLLVGFRNTLDAGCAFMLISLGVVVVVEAAQNGPGSLVFP"
     gene            977..1888
                     /locus_tag="VFT03_00430"
     CDS             977..1888
                     /locus_tag="VFT03_00430"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HEU4846635.1"
                     /translation="MRGTDGRAGRAGVMVPEASYLICATPRSGSTLLCDALESTGIAG
                     HPAEHFEVLLETGLPRQPRDYFQRSNDPEVWALLDDREFQDVLGEYGGRYADHLALRD
                     PSWQPPHFEGLLAEALRKGTTENGVLGTKIMWAYFRDFVRLARRTPGLEDVRPCDVPG
                     AVLPNLRAHVWIRRQDTTRQAVSLWKALQTQQWRRDSDEEAGGRGLRFSFAAVNHLKL
                     RIDEHNAAWQGFFEECGAEPLKVVYEELIEDYEGTVLRLLDGIGVPVHENFVVEMPKM
                     KRQADELSEEWVSLYNERAAAKTAHRG"
     gene            1892..>2321
                     /locus_tag="VFT03_00435"
     CDS             1892..>2321
                     /locus_tag="VFT03_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU4846636.1"
                     /translation="MRDLPNILYVHSHDTGRWVRPYGHAVRTPNIQRLAEEGVIFRKA
                     FCAAPTCSGSRACLLTGQYAHSNGMVGLAHRGFSLNDYRHHIVHTLHELGYWSALVGE
                     QHISKMPEVIGYDEVIKIDTSHVDDVTPVTIDLLTKRRKEP"
BASE COUNT          445 a          756 c          720 g          400 t
ORIGIN      
        1 gcggtaggta gaggcacggt agtaggaggc cacggttggt ggcgggaggc gcagcatggt
       61 gcgcctccac gcgcctctac aagcgccgat acgctacggg aataccaggg acccgggccc
      121 gttttgggcg gcttcgacca ccaccacgac gccgagcgag atcaacatga acgcgcagcc
      181 ggcgtcaagc gtgttcctga accccaccag cagcggaaag agcatccagg cggtgagcac
      241 cgtaccgtac aacaccgccc cggcgccggc gatcgtggag accaccaccg agaatagcag
      301 taagcctacg accgccgaat cgagcgccgc ccggagtcga tctgccctca gaacgctacg
      361 gtccatcacc gcttatcaac cacgcgtagt actcgccccg cgcctctttg ccgtcgagcc
      421 gtgcgaggtg atcctcgacc caagagtaac gacggccctc ctccagtccc cggaccggct
      481 catccacttc gcgttttccg gctcgcgttg cttcgacgac cctaccgccc cacaccagcg
      541 cgctcgtcct cactcccggc caccatcggc gggctctgac ggggcagacg cctcatccag
      601 aacatctacg tcgggaggcg aatgagcgtc ccgcccgcct ccccttcgct tcccgtgcgg
      661 aagctcgtac caggtgtcca aaccctggct gcccgcccga tcccacatct cgtaccagag
      721 atccagctcc cacttcacgc ccaataacct cctcttcatc atttcctgta ttgtctatta
      781 cttttataga caatacatac atggtatcct gccggcggct ctggcgtcaa gaggatgcga
      841 gatcaattta cgcgggactt ttgtcccagt accgatggtc accaccgtag cggtggaccg
      901 ccagacattt tttcactaat cttgtagatt aagttggctt atcatcagag ttgtcccaga
      961 cgagaggagg cagagcgtga gaggaacgga tggtcgggcg gggcgagcgg gcgttatggt
     1021 gccggaggcg tcgtacctca tctgcgcgac gccgcgcagc gggagcacgc ttttgtgtga
     1081 tgctctggag agcacgggta tcgcgggcca tccggcggag cacttcgagg tattgctgga
     1141 gacggggctg ccgaggcagc cgcgcgacta cttccagcgc tccaacgacc ccgaggtatg
     1201 ggcgctgttg gatgaccgcg agtttcagga cgttctcggc gagtacggtg gccggtacgc
     1261 cgaccatctg gcgctcaggg acccctcatg gcaaccgccc catttcgagg ggttgctggc
     1321 ggaggcgctg cgcaaaggca ccacagagaa cggcgtgctg gggaccaaga tcatgtgggc
     1381 ctacttccgc gacttcgtgc gcctggcgcg gcggacgccg ggactggagg acgtcaggcc
     1441 gtgcgatgtg cctggggcgg tactgcccaa cctgcgggcg cacgtatgga tccggcgcca
     1501 agacaccacg cgccaggcgg tctctctgtg gaaggcgctc cagacccagc agtggcgcag
     1561 ggactccgac gaggaagccg gcggtcgggg gctgcgtttc agcttcgccg ctgtgaacca
     1621 cctcaagctg cgcatcgacg agcacaacgc cgcctggcag ggcttcttcg aggagtgtgg
     1681 cgcggagcct ttgaaggtgg tctacgagga gctgatcgag gactatgagg ggaccgtgct
     1741 gcggctgctc gacggcatag gcgttccggt tcacgagaac ttcgtcgtcg agatgcctaa
     1801 aatgaagcgg caggcggacg agctctccga ggagtgggtc agcctgtaca acgagcgcgc
     1861 ggccgccaag accgcgcata gggggtaaga attgcgggat ctaccgaaca tcctctacgt
     1921 ccactcccac gacacgggca ggtgggtcag gccctacgga cacgctgtca ggacgcctaa
     1981 catccagcgt ctggccgaag agggtgtcat cttccgcaag gccttctgcg ccgctccgac
     2041 ctgttcgggg agccgcgcct gcttgctgac cggccagtac gcccactcca acgggatggt
     2101 cggcctcgcc caccgcggct tctcgctcaa cgactatcgc caccacatcg tccacacgct
     2161 gcacgagctc ggctactggt cggcgctcgt cggcgagcag cacatctcca aaatgcccga
     2221 ggtgatcggc tacgacgagg tcatcaagat cgacaccagc catgtcgatg acgtcacgcc
     2281 ggtaacgatc gacctcctga caaagaggcg taaagagccg t
//
LOCUS       DASTUV010000023         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_818962, whole genome shotgun sequence.
ACCESSION   DASTUV010000023 DASTUV010000000
VERSION     DASTUV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818962"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFT03_00440"
     CDS             <1..922
                     /locus_tag="VFT03_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846637.1"
                     /translation="HIPFVDYARGDGMSIGAGGEKAWDPPRLMLDPPPKWVSGYRGMW
                     GLYTRDPFEGEDAPAGPMYSRDKSVNRAWYDPVGWAGLDKVPPPDETLDAVLGRRADL
                     EARCAALRTEIDEKSRHLKELGVEISALRDRSHLDEPYEEGKRRIAQLSREADRLRAR
                     LATDEAVSASLEEYADRLRAGERDPARAHISRAHRPASEDELRVSRVAEAWAAASVSL
                     MLISFVGIVLFEREHLISMLVFSIALFAFVEAGFRGRLVNLVSSVNIGLAVVAALILL
                     YEFFWQLVVAGVLGVGLYVLWDNLRELRRR"
     gene            927..2297
                     /locus_tag="VFT03_00445"
     CDS             927..2297
                     /locus_tag="VFT03_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4846638.1"
                     /translation="MDLISELRSILADPESVRDDGGEVERHGSSVFTYHDPHPPDAVV
                     YPKSRDEVAEILRFADDRGVPVIPFGQGSSLEGQTIPARGGISLDFALMDGILEVRPD
                     DFLARVQPGVTHEKLNAHLREHGLFFPVDPGWDASLGGMAATNASGTNAVRYGAMRDQ
                     VLGLEVVLASGTVMRTGGMAMKSSAGYNLTALFVGSEGTLGVFTEIILRLYPIPERVL
                     AARAVFAGIERAGSAAVAMIRSGMQVGRVELVDARTIEAVNAYKGTAYAPSPTLFLEF
                     SGTEAAVEHDVVAARVLCEAEGAQGFEFEEDEAARERLWEARHEAALAIKELHPGRRP
                     MTTDVCVPISELPGALVHARDAIASRGFEGAILGHVGDGNYHAVFPVDTRDAADLQRA
                     RAVNAEIVDYALERGGTCTGEHGVGSGKTEDLRKEHGDSLPFMRAIKRLADPNGVLNP
                     GKIFPA"
     gene            2338..>2684
                     /locus_tag="VFT03_00450"
     CDS             2338..>2684
                     /locus_tag="VFT03_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU4846639.1"
                     /translation="MARTIRVVVAKVGLDGHDRGAKIIARALRDAGMEVIYTGLHQTP
                     EQVVETAIQEDADAIGISILSGAHMTLIPRIIELLKENEAEDILVFCGGTIPNDDIKR
                     LKEMGVGEIFTPGT"
BASE COUNT          465 a          848 c          956 g          415 t
ORIGIN      
        1 ccacatccca ttcgtcgact acgccagggg ggatgggatg tccatcgggg cgggtggcga
       61 gaaggcgtgg gacccgccac gcctgatgct cgacccgccc ccgaagtggg tctcgggcta
      121 cagggggatg tgggggctct acacccgcga ccccttcgaa ggcgaggacg caccagcggg
      181 tcccatgtac agccgggaca agagcgtcaa ccgggcctgg tacgaccctg tggggtgggc
      241 cgggctggac aaggtgcctc ccccggacga gacgctcgat gcagtgctcg gccggcgcgc
      301 cgatctggag gcgcggtgcg cggcgctacg gaccgagatc gacgagaaga gccgccacct
      361 gaaggagttg ggcgtggaga tctcggccct gcgcgacagg tcccacctgg acgagcccta
      421 cgaggagggg aagaggcgca tcgcgcagct ctccagggag gcagatcggc tgcgggcgcg
      481 cctcgccacc gacgaggccg tctcggcctc tttggaagag tacgccgatc ggttgagggc
      541 cggggagcgg gaccccgcgc gagcccacat ctcccgcgct caccggcccg cctcggagga
      601 tgagctgagg gtgagccgcg tggccgaggc gtgggcagcc gcgagcgtga gcctcatgct
      661 tataagcttc gtcgggatag tgctcttcga gcgggagcac ctcatctcca tgctcgtctt
      721 cagcatcgcc ctgttcgcgt tcgtcgaggc cggcttcagg ggacggctcg tcaacctggt
      781 gagcagcgtc aacatcgggc tggcggtggt cgcggccctg atcctgttgt acgagttctt
      841 ctggcagctc gtcgtcgccg gagtcctggg cgtgggcctc tacgtcctct gggacaacct
      901 gcgcgagctc aggcggaggt gaggaaatgg accttatttc agagttgaga agcatcctgg
      961 cggaccccga aagcgttcgc gacgacgggg gagaggtcga gcgccacggg agcagcgtct
     1021 tcacctacca cgacccccat cctcccgacg ccgtggtgta cccgaagagc agggacgagg
     1081 tagcggagat actgcggttc gcggacgatc gtggcgtccc cgtcattccc ttcggccagg
     1141 ggagcagcct ggagggccag acgatcccgg ctcgcggcgg gataagcctg gattttgcgc
     1201 tgatggatgg gatcctcgag gtccgccccg acgatttcct ggcccgggtg cagcctggcg
     1261 tgacgcacga aaagctcaac gcgcatctga gagagcacgg gctcttcttc cccgtggatc
     1321 ccggctggga cgcctcttta ggcgggatgg cggcgaccaa cgcgagcggg acgaacgccg
     1381 tgcgctacgg agcgatgcgg gaccaggtcc ttggcctcga ggtggtgctc gcgagcggga
     1441 cagttatgcg caccggcgga atggcgatga agtcctcggc cgggtacaac ctgaccgccc
     1501 tcttcgtcgg ctccgaaggc acccttggcg tgttcacgga gataatcttg cggctgtatc
     1561 ctatcccgga gcgcgtcctc gcggcgcggg cggtcttcgc tggcatcgag cgggcgggaa
     1621 gtgctgcggt cgccatgatc cggtccggga tgcaggtcgg gcgtgtcgag ctggtcgacg
     1681 cccgcaccat cgaggcggtg aacgcctaca agggcacagc ctacgcccct tctccgacgc
     1741 tcttcctcga gttctcgggc acggaggcgg ccgtcgagca cgatgtggtg gcggcgcgcg
     1801 tactctgcga ggcagagggc gcgcaagggt tcgaattcga ggaggacgag gccgcaaggg
     1861 agcgcctgtg ggaggcccgc cacgaggccg ccctcgccat aaaggagctg caccccggca
     1921 ggaggccgat gaccaccgac gtgtgcgtcc cgatctccga gttgcccggt gctctagtcc
     1981 acgcccgcga cgccatcgcc tcgcgcggtt tcgaaggggc catcctcggg cacgtcggcg
     2041 acggcaacta ccacgcagtc ttccccgtgg acacacgaga cgcggcggac ctccagaggg
     2101 ccagagcggt caacgccgag atcgtcgact acgccctcga gcgcggcggc acctgcacgg
     2161 gcgagcacgg cgtcggctcg ggcaagacgg aggacctgag gaaggagcac ggcgactccc
     2221 tgcccttcat gcgtgccata aagcggctcg ccgacccgaa cggcgtgctc aacccgggca
     2281 agatcttccc cgcctaggtg tcgcgcgggg agtggatgcg gttagaatgg gtgcagaatg
     2341 gcgcgcacga taagggttgt ggtagctaag gtagggctcg acggccacga caggggggca
     2401 aagatcatag cccgtgccct gagggacgcc ggtatggagg tgatctacac cggcctgcac
     2461 cagaccccag agcaggtcgt cgagacggcc atccaggagg atgccgacgc catcgggatc
     2521 tccatcctat cgggcgccca catgaccctg attccccgga tcatcgagct cttgaaggag
     2581 aacgaggccg aggacatcct ggtcttctgc gggggaacca tcccgaatga cgacatcaag
     2641 aggctcaagg agatgggggt cggcgagatc ttcacccccg gaac
//
LOCUS       DASTUV010000024         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1140241, whole genome shotgun sequence.
ACCESSION   DASTUV010000024 DASTUV010000000
VERSION     DASTUV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140241"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFT03_00455"
     CDS             complement(<1..446)
                     /locus_tag="VFT03_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846640.1"
                     /translation="MRRSNVRAVREEEFTSLYRQNDPQAAAWAAGYGPIEHAVETQAR
                     VFRMAHLLEARGVRGDGATLFEVLHAADRAACAAMWLVVHETYARNVYLDGRDLGPED
                     FKTKPEGHTGGALNMVPAYIGYMAVNAITGLTRSWVMGQGHTVAAI"
     gene            complement(450..1703)
                     /locus_tag="VFT03_00460"
     CDS             complement(450..1703)
                     /locus_tag="VFT03_00460"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU4846641.1"
                     /translation="MLAINCGSSSLKFRLSETPVASPDAEETLAWGSVEGMGRRATVD
                     LATAEGSTLREAAEVADHGAAVERVLGWLGEHGLAGTIEAVGHRVVDGGSRFAAPTRV
                     DDDVVAAIEELGELAPLHNGPALAAIGATRDALGPAVPMVATFDTTFHRQMPEKASRY
                     AIPPELAERHGAFRYGFHGLAHRYMAERYAEITSTSEAKLVTLQLGNGCSAAAIRDGR
                     SVDTSMGLTPLEGLMMGTRSGDVDPSLAAYLARRESVEVAEVVTWLNKRSGLLGLSGT
                     SRDMRELLAARNRGDGRAALAVEMFCYRVKKQIGAYLAALGGADAVVFAGGIGERAFE
                     VRAEICSGMQWCGLVLDPERNAGAVGEETRISADDSSVAAYVIPVDEAAIIARDTVDC
                     LLREGYTVEPAAAEREQPNGEFRRM"
     gene            complement(1706..2986)
                     /locus_tag="VFT03_00465"
     CDS             complement(1706..2986)
                     /locus_tag="VFT03_00465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4846642.1"
                     /translation="MLESVDVGRQSIGDYEGTTGREAVDRLRSLAEPLRGARLLHLNA
                     TPYGGGVAEILRSEVPLLRDLGLEAEWKIITGDKDFFSVTKTMHNALQGARRTLTDHE
                     KEIYLTYSTRNAHLLEEEYDLIVAHDPQPLAIPYFHGRGGTRWIWRCHIDTSEPNPEL
                     WDFLRPYLDHHDAAVFTLDGFVPSEFPISRVEVIPPAIDPESPKNIELSSKTTERLLG
                     WLGVEFGKPLVTQVSRFDPWKDQPGVIEAYRLVKREVPDLKLALVGSMALDDPEGWEV
                     YRQIKDSAREDPDIDLFTNLTGVSNVEVNAFQRCSDVCVQKSIREGFGLVVSETLWKG
                     TPMVAGEAGGIPLQMQGGAGGFLVDSVEECAEKMLRLLHNPEEGEELALWGKEIVREH
                     FLLPRLIADELELYTSLLSTGTQPGAAPAPEVRP"
BASE COUNT          525 a         1050 c          996 g          580 t
ORIGIN      
        1 tctatggcgg caacggtgtg cccctggccc atgacccacg atcgcgtgag gcccgtgatg
       61 gcgtttaccg ccatgtaccc gatataggcg ggcaccatgt tcaaggcgcc tccggtatgc
      121 ccctccggct tcgtcttgaa gtcttccggc cccaggtccc gcccgtcgag atagacgttc
      181 ctcgcgtaag tttcgtgtac gacgagccac atcgcggcgc atgccgcgcg gtcggcggcg
      241 tgcaagacct cgaagagggt cgctccgtcc ccgcgcaccc cccgggcctc gagcaggtgc
      301 gccatccgga acacgcgggc ctgggtctcc accgcgtgct cgattggtcc gtagcccgcc
      361 gcccaggccg ccgcctgcgg gtcgttctgg cggtagaggg aggtgaactc ctcctcgcga
      421 accgctctga cattgcttcg acgcatgacc tacatcctcc tgaactcgcc gttgggttgt
      481 tctcgctccg cggcggccgg ctcgactgta tagccctcgc ggagcaagca atctacggtg
      541 tcgcgggcga taatagccgc ctcgtccacc gggatgacgt aggctgccac ggacgagtcg
      601 tccgcgctta tcctggtctc ttcaccgacg gccccagcat tgcgttcggg gtccagaacg
      661 agcccgcacc attgcatacc ggagcagatc tcggcccgca cctcgaaggc gcgctccccg
      721 atgccaccgg cgaagacaac tgcgtccgct cctccaaggg ccgcgagata cgccccgatc
      781 tgcttcttca cccggtagca gaacatctcg accgcgagtg ccgcccgtcc gtccccccgg
      841 ttcctcgccg cgagaagctc cctcatgtcc ctagaggttc cggagagccc gaggagtccg
      901 gagcgcttgt tcagccaggt aacgacctcg gcaacctcga cactctcgcg gcgggcgagg
      961 taggcagcta gcgagggatc cacgtcgccg cttcttgtgc ccatcatcag cccctcgagc
     1021 ggcgtgagtc ccatggaggt gtccaccgag cgtccgtcgc ggattgccgc ggcggagcag
     1081 ccgttcccga gctgcagggt gacgagcttg gcttccgaag tggacgtgat ctcggcgtag
     1141 cgctcggcca tgtagcggtg ggcgagaccg tggaagccgt agcggaaggc accgtgcctc
     1201 tcggccaact ccggaggtat ggcatatcgg gaggccttct ccggcatctg tcggtggaag
     1261 gtggtgtcga aggtcgcgac catcggcaca gccggtccca gggcatcgcg cgtggccccg
     1321 attgccgcca gggccggccc gttgtgcagt ggagcgagct ccccgagttc ctcgatcgct
     1381 gccaccacat cgtcgtcgac gcgcgtgggg gcagcgaaac ggctaccccc gtccacgacg
     1441 cgatgcccca ccgcctcgat ggtccccgcg agcccgtgct cgccgagcca tccgagcacc
     1501 cgttccactg ccgcgccgtg gtcggcgacc tcggctgcct cccgcagggt agacccttct
     1561 gcggtggcga gatccactgt tgctcggcgg cccataccct cgaccgagcc ccaggccaga
     1621 gtctcttctg cgtcagggga tgccacggga gtctcagaga gccggaattt cagcgacgac
     1681 gagccgcagt tgatagcgag caccctcaag ggcggacctc cggcgctgga gcggcgccag
     1741 gctgtgttcc tgtgctgaga agtgaggtgt agagctcgag ctcgtcggcg atgaggcggg
     1801 ggaggaggaa gtgttccctg acgatctcct tgccccacag tgcaagctct tcgccttctt
     1861 cgggattatg gagaaggcgc agcattttct cggcgcactc ctcgacggag tccacgagaa
     1921 aaccgccggc tccgccttgc atctgcaagg ggatgccccc ggcctcgcca gcgaccatcg
     1981 gggtcccttt ccacagggtt tcggagacga cgagcccgaa gccttcccgg atcgacttct
     2041 ggacgcatac gtcggagcag cgctggaagg cgttcacctc gacattgctc acgccggtca
     2101 ggttagtgaa tagatctatg tccgggtctt cgcgggcgga gtccttgatc tgcctgtaga
     2161 cctcccagcc ttccggatcg tcgagcgcca ttgagcccac cagcgccagc ttgaggtcag
     2221 gcacctcccg cttcaccagc cggtacgcct ctatgacgcc tggctggtcc ttccagggat
     2281 cgaaccgtga gacctgggtg acgagtggct tgccgaactc gaccccgagc caacccagca
     2341 gccgttcggt ggtctttgaa gagagttcga tgttcttcgg gctctccggg tcaatcgcgg
     2401 gcggtatgac ctcgactcgc gagatcggaa actccgaggg tacgaacccg tccagggtaa
     2461 agaccgccgc gtcgtggtgg tcgaggtagg ggcgtaagaa atcccagagc tcggggttgg
     2521 gctccgaggt atctatgtgg cacctccaga tccacctcgt gcctccgcga ccgtggaagt
     2581 aggggattgc caggggctgg gggtcgtgag cgacgatcag atcgtactcc tcctcaagca
     2641 ggtgtgcgtt gcgggtggag taggtaaggt atatctcctt ctcgtggtcc gtgagcgtgc
     2701 gcctggcgcc ctgcagagcg ttgtgcatcg tcttggtgac cgagaagaag tccttgtctc
     2761 cagtgatgat cttccattcg gcctctaggc ccaggtcccg cagaaggggc acctcggagc
     2821 gcaggatctc cgcgaccccg ccgccgtagg gcgtggcgtt caggtgcagc agccgcgcac
     2881 ccctcagggg ctcggccaga gagcggagtc gatcgaccgc ctcgcggccg gtggtcccct
     2941 cgtagtcgcc gatagactgc ctgccgacgt ctacgctttc aagcaccacg cctccttgga
     3001 ccttggagac cgtccaaaaa cacttgtcct gaaggctacc atcgtgcggc cagaagcagg
     3061 actagtgcga tcacaaacaa tgccgtgaac actcgtttga agcctctaaa gtgcaaccat
     3121 tcctggaaga agatgtcgct gccctcggta a
//
LOCUS       DASTUV010000025         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1643705, whole genome shotgun sequence.
ACCESSION   DASTUV010000025 DASTUV010000000
VERSION     DASTUV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643705"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..769
                     /locus_tag="VFT03_00470"
     CDS             200..769
                     /locus_tag="VFT03_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4846643.1"
                     /translation="MRTRDALIAAARQLLAEGVIPTIEEAASEASVGRTTAYRYFPNG
                     RALLAATFPEIEMDSLLGADPPEDPLARLEVVAESLTRGIVEHEPEYRAQLRLALEGE
                     PAGGESLPLRRGRRIGWVEDALAPLRGRMPQQELRRLVYGISATLGIEALVWLTDVGG
                     LSREEAVEVMRSNARTLLCSALERLESSV"
     gene            1486..2235
                     /locus_tag="VFT03_00475"
     CDS             1486..2235
                     /locus_tag="VFT03_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846644.1"
                     /translation="MLRKILYGMATSALLALLPVVAVAQNSTAQPVDPSRQVAQLRQK
                     FEGLTPQQIEAQGFVVEGSCVPSPKGAGAMGIHAINRQLYQAQFPKGEMDPENPPVIL
                     LGQNDQVIGVEWEAKDVGQGPMKMFGQTIELQSGHPGVPEPHYMLHIYFKSGGKVLFG
                     TNDQTAFDPEGVCPEMSAMSAMSAHSTASSTATASASASSMATSSASATSSASALTQT
                     GGMSPMLVLGAGALVLLLAGGLMAATLVRRS"
     gene            2471..>3162
                     /locus_tag="VFT03_00480"
     CDS             2471..>3162
                     /locus_tag="VFT03_00480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4846645.1"
                     /translation="MPPRLYDDDPLTPGERDELEESLISFSDRELDLLGDIRGLDVLY
                     AGGASPLWIEGLSQRLGEAGNLTALEAEAERISEGRETLRDAALASPVRLVVGNVFEP
                     PFGPQTFDLVYSAGLFHELDVRERPAEDALRSLISVVRPGGRVATSDFIDSVPAVQLA
                     DEEIQRELALEASGARLYGIGSPERLVGLHESLLEAVRWRVSLPYEVRHLDELVLAEG
                     EPEGLSHLSAGA"
BASE COUNT          585 a          964 c         1011 g          602 t
ORIGIN      
        1 agcgctgctc tccctctccc tccccgcgga cgatcggtac cgcagtgtcc attgctctct
       61 cctccttacg cgccttcggc acgcttgcct tttatgggac aaacattacc atgatggaca
      121 taattgttca atagagaaaa cgtgatacta tgatggaatg gacgttccat atctgcgtac
      181 aggacgcacc agtcagaagg tgcgtacgag ggatgccctg atcgcggccg cccggcagct
      241 ccttgctgag ggggtaattc ccaccatcga ggaggcggct agtgaggcgt ccgtcggtcg
      301 caccacggcc taccgctact tccctaacgg tcgggcgctt ctcgccgcta ccttcccgga
      361 gatcgagatg gactcgctgc ttggagcaga cccgcccgaa gacccgctgg cgcggctcga
      421 ggtcgtagcc gagagcctga cccgggggat cgtcgagcac gagcccgagt accgagccca
      481 gctgcggctc gcgctcgaag gcgaacccgc gggtggtgag agcctgccgt tgcggcgagg
      541 gcgacggatc ggatgggtcg aggatgctct ggcccccctg cgcgggcgaa tgccgcaaca
      601 agaactgcgg cggttggtct acgggataag cgcgacgctc ggcattgagg ctttggtatg
      661 gctgaccgac gtgggcgggc tttctcgcga ggaagcggtg gaggtgatgc gctcgaacgc
      721 tcgcacgctg ctgtgttccg cgcttgagcg actagagagt agcgtgtaac acagcagcgt
      781 gacggcggct cctttttcgg cgatctcccg gcgtgcaaga gccggacttc catcgcgccc
      841 ggtgctgtgc agttgccttg gtaatcacag tcctttgaga ctattgttag tggaggcgac
      901 gttttgaaac cggttcctac tcggcggcct ggtcgccctc tactccctca aggacaggct
      961 gggtgttggc gcaggactcg cgcccttcgg cctccttcac agtcctccag gaggttgacg
     1021 gcatagcttt gaagcgggcg gtcgaatacc cagggcagac gatggccggg gaaaggagaa
     1081 cgaagccatg gggaaaatcc gcactggaca ctcaacttcg ctggacgggt ttatcgccgg
     1141 accgaacgac ggctctgaga atcccatggg tgagggtggc gagcgactct tagcgtggta
     1201 ctccggcggc gatacgacca ggtcttttac ggacgtccgc aaggtcttga ggtgttctcc
     1261 aacgctcacg tcgaccatcc cggcgacctc ttcgagagtg tttggcacct gtaaccgtta
     1321 ggggtagcta ccactcagct tcgggcgtcc ccgagagttc gctacccgca agcaacgccc
     1381 aaggaacagc aaaaatacgt tatttggggg atgtactgct ccttgctggg gaataagctg
     1441 cagacgttcc gcgccgtcag catcattact gggaggagaa ctatattgtt acgcaagatc
     1501 ctatacggta tggcaacaag cgctctgcta gcgctactgc ccgttgtggc agtcgctcag
     1561 aacagcaccg ctcagccggt agacccgtcc cggcaggttg ctcagctgcg ccagaaattc
     1621 gaagggctta cgccacagca gatagaggcc caaggttttg tggtcgaggg ctcgtgcgtc
     1681 ccgagcccta agggtgccgg ggctatgggc atccacgcca taaaccggca actctaccag
     1741 gctcagtttc ccaaggggga gatggatcct gaaaacccgc ccgtgatctt gcttggtcag
     1801 aacgaccagg tgatcggagt agagtgggag gccaaggacg tggggcaggg accgatgaag
     1861 atgttcgggc aaaccatcga gctacagtcg ggacatccgg gggtgcccga acctcactac
     1921 atgctgcaca tctacttcaa gtccggtggc aaggtgcttt tcggcacgaa cgaccaaacc
     1981 gcgttcgatc ccgagggcgt ctgtcccgag atgtcagcga tgtcagcgat gtctgctcat
     2041 tccaccgcga gttctacagc aaccgcgagc gcatccgcga gctcgatggc cacttcaagc
     2101 gcgagcgcaa ccagcagcgc gtccgcgctc acccagaccg gcggcatgtc gcccatgctc
     2161 gtgctagggg ctggcgctct agtgctgttg ctcgccggcg ggctcatggc ggccactcta
     2221 gtccgtcgga gctagcagat aacgtcgttc gaagggccgg agactggact ccggcctttc
     2281 tttatggcgt catgcggcca cttgcacgta catcgtgccc gttgtgcagg ttgtagggtc
     2341 gagtacgctt gtcgagggat ggtgacggta cagtcggtag ctagctcctc gccaactcca
     2401 accgctcggc gacgtcctcg gtgagcaggg gcgcgaaatg ggaagggact ttctgcagga
     2461 tgggaatttg atgccgccga gattatacga tgatgatccc ctgacaccgg gcgaacgaga
     2521 tgagctcgaa gagagcctca tctcgttctc cgaccgcgag ctcgacctcc tcggcgacat
     2581 ccgcggcctt gacgtactct acgccggcgg cgcctcgccg ctctggatcg agggcttgag
     2641 ccagcgcctc ggtgaagcag gcaacctcac cgctctcgaa gccgaagccg agcgtatcag
     2701 cgaagggcgg gaaaccctcc gagatgcggc actcgcctca ccggtccgcc tcgtcgtagg
     2761 gaacgtgttc gagccgccct tcgggccgca aaccttcgat ctggtctact ccgccggcct
     2821 cttccacgag ctggacgtgc gtgagaggcc ggcggaagac gccctgagat ctctcatctc
     2881 cgtggtgcgg cccggcggaa gggtcgccac cagcgacttc atcgattccg tgccggcggt
     2941 tcagctcgcc gacgaggaga tccagcgcga gcttgcgctc gaagcctcgg gcgcccggct
     3001 ctacggcatc ggctcgcccg agcggttggt cgggctgcac gagtcgctgc tggaagccgt
     3061 gcggtggcgc gtctcgctgc catacgaggt ccggcacctg gacgaacttg tcctcgccga
     3121 aggagagccg gaggggctgt cgcacctgtc cgccggcgcg ca
//
LOCUS       DASTUV010000026         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_461351, whole genome shotgun sequence.
ACCESSION   DASTUV010000026 DASTUV010000000
VERSION     DASTUV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461351"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..2632)
                     /locus_tag="VFT03_00485"
     CDS             complement(203..2632)
                     /locus_tag="VFT03_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4846646.1"
                     /translation="MREPGAPLRVLLVEDSENDALLFLRELRRGGYEPLYEQVSTPEE
                     MERALGEASSRGEPWEVVVSDYYVPRFGALAALELLRRLGYDTPFVVVSGKIGEDAAV
                     AMMRAGAQDYVTKEDMVRLCPAIERELREAEGRRERKRSAEALRFLSEASAALTSSLD
                     YRATLARVARLAVPQLADWCAVDVREEDGSTHQLAVEHENPEKVAWAHELQQRYPPDA
                     DAPRGVPEVLRSGRSEFYPDVTDEMLVAAARDPEHLRLMRRMSFTSAMVVPLVARGRT
                     LGAITLVSAESGRRYDSADLELAEELARRAALAVDNARLYDEAQKEISERNRAETRFR
                     TLVEQIPAVTYIQEPIESDNPKVVTYMSPQYETMLGYPREAEILDEEHWLRTLHPEDR
                     ERVLAEEVRTDESGEPFKIEYRVIAADDRVVWVRDHAVLMRDKEGQPLYWLGVQYDIT
                     EQKRVEAALRQSEELYRTVVEQAAENIFLVDVETRRVLEANAALRRSLGYTPEEFQDL
                     TLYDIVAHDRQSVDQNTQRILEEGQRYLGERRYRRKDGSLVDVEVSASAIYYDGGEAM
                     SVVAHDITERKKAEEALDDAREAERNRIARDLHDDILQDIVYALQEIQVAQITSENGG
                     SEALEEAADALRRSVEGLRDAIFELRLKQTLGRSFVSSLENLMDLNRRMARSQYELDL
                     VVADGFPSHFPERTGREIVRIIQEALTNARRHARPRRVLVRLWRDGDLARVEVADDGR
                     GFDTDRAGIGVGQQSMRYRALELGGELTVESTPGGGTRVRFEVPVSRLLEEVRPGRGG
                     QPSGDEGTSKA"
     gene            complement(2629..>3784)
                     /locus_tag="VFT03_00490"
     CDS             complement(2629..>3784)
                     /locus_tag="VFT03_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4846647.1"
                     /translation="QDTVAERRRALKGFVVGFFRWDGLLDGVFGGGFDPAIDFELYDS
                     REVGSSERLYDSDGVQRAGKPEVETLFSEERQMEVAGREWSLYFATLPGFEKEAGSNL
                     PGFVLASGVAVSLLLFGITLLLVRNSTLAERASKDLEDANRELEGANRELEAFSYSVS
                     HDLRSPLRSIDGFSRILLEDYADKLDEEGADYLGRVRGASKHMALLIEDLLDLSRVSR
                     GPLRREVIDLSAMAKDIVKKLERAHPERRVKFVAQEGVVAFGDANLLAVALENLLGNA
                     WKFTSKRDEATIEFGVDVPHGGGSVAYYVRDDGAGFDPRYADKLFGAFQRLHGSEEFE
                     GTGIGLATVARIVHRHGGRVWAESQIGEGATFYFTLGGRQEGSPRKADVW"
BASE COUNT          579 a         1375 c         1128 g          702 t
ORIGIN      
        1 cagcacctct ccgttcagct gccgcgcctc ggcaagcgag ccaacctgcc ctgccacctc
       61 gaacccgggc tccctctcaa gcatgaaagc tagagcctgg cggaatgaag catgatcctc
      121 gactaacagg actctgatcg ggctgtcttt gctatggttc tctatggcag tcatcccttt
      181 ctatccggtg tgtaccagcg aattatgcct tcgacgtgcc ttcgtcgcca gaaggctgtc
      241 ctccccgtcc cggacgaacc tcttcgagca ggcgcgagac gggtacctcg aagcgcactc
      301 gcgtcccgcc gcccggcgtg ctctctaccg tgagttctcc gccgagctcg agagccctgt
      361 acctcatcga ctgctggccc actccgatcc cggctctgtc tgtgtcgaag ccgcgcccgt
      421 cgtcggccac ctctacgcga gcaaggtcgc cgtcgcgcca gagcctcacc aggacgcgtc
      481 ggggccgcgc gtggcggcgg gcattggtga gcgcctcctg gataatgcgt acgatctccc
      541 ggccggtcct ctcggggaag tgactcggga acccatctgc cacgaccagg tccagctcgt
      601 actgactccg cgccatccgg cggttcaggt ccatgagatt ctccagcgaa gagacgaacg
      661 accggccaag cgtctgcttc aagcgaagct cgaagatggc gtcgcgcagc ccctccaccg
      721 agcgccggag tgcgtcggcg gcctcttcga gagcctcaga cccgccgttc tccgaggtaa
      781 tctgggcgac ctggatctcc tgcaaagcgt agacgatgtc ctggagtatg tcgtcgtgga
      841 ggtcgcgggc tatccggttg cgctcggcct ccctcgcatc gtccagcgcc tcctcggcct
      901 tcttgcgctc ggtgatgtcg tgggcgacga cgctcatagc ctcgccgccg tcgtagtaga
      961 tcgcgctggc gctgacctcc acgtcgacca gagacccgtc cttgcggcgg tagcgcctct
     1021 cgccgagata gcgttgtcct tcctcgagga tgcgctgcgt gttctgatcc acgctctgcc
     1081 tgtcgtgggc aacgatgtcg tagagggtca ggtcttggaa ctcctcgggc gtatagccaa
     1141 gcgagcggcg cagcgcggcg tttgcctcga gaacccgcct ggtctccacg tccacgagga
     1201 agatgttctc ggccgcctgc tccaccacgg tgcggtagag ttcctcgctc tggcgcagcg
     1261 ccgcttcgac ccgcttctgc tccgtgatgt cgtactggac gccgagccag tacaggggct
     1321 gcccttcctt atctcgcatc agcactgcgt gatccctcac ccacactacc cggtcgtccg
     1381 ctgcgatgac gcggtactct atcttgaagg gttcgccgct ctcgtcggtc cgaacctcct
     1441 cggcgagcac ccgctcccgg tcttcgggat gcagtgtcct gagccagtgc tcctcgtcga
     1501 gtatctctgc ctcccgaggg tatccaagca tggtctcgta ctgtgggctc atgtaggtta
     1561 cgactttggg attgtccgac tcgatgggct cctgtatata ggtgaccgcc gggatctgct
     1621 cgaccagcgt gcggaagcgg gtctcggccc tattgcgctc ggagatctcc ttctgtgcct
     1681 cgtcgtagag cctggcgttg tccaccgcca gagctgcgcg acgggcgagc tcctcggcca
     1741 gctccaggtc cgcgctatcg taacgccgcc ccgactccgc cgagacgagc gtgatcgccc
     1801 ccagcgtgcg tccgcgggcg acgagcggca ctaccatcgc ggaggtgaag ctcatccggc
     1861 gcatgagccg gaggtgctcg ggatccctgg ccgcagctac aagcatctca tccgtgacat
     1921 ccgggtagaa ctcggagcgc ccgctgcgca agacctccgg aaccccccgc ggagcgtccg
     1981 catcgggcgg atagcgctgc tgcagctcgt gcgcccacgc taccttctcc gggttctcgt
     2041 gctccaccgc cagctggtgc gtgctcccgt cctcctcccg gacgtccacg gcgcaccagt
     2101 cggccagctg gggcaccgcc agacgggcca cccgggccag cgtggcgcgg tagtcgagcg
     2161 aagaagtgag cgccgcgctc gcctcggaca ggaagcgcaa cgcctccgcc gagcgtttgc
     2221 gctcccggcg cccctccgcc tccctgagct cccgttcgat cgccgggcac agccgcacca
     2281 tgtcctcttt cgtgacgtag tcctgcgccc cggcccgcat catcgcgacc gcggcgtctt
     2341 caccgatctt gcccgagacg acgacgaacg gcgtgtcgta gcccagccgc cgtagcagct
     2401 cgagggcggc gagcgccccg aaacgcggca cgtaatagtc ggatacgact acctcccacg
     2461 gctccccccg cgagctcgcc tcgccgagcg cccgctccat ctcctcgggg gtgctgacct
     2521 gctcgtagag gggctcgtag ccgccgcggc ggagctcgcg caaaaagagc agcgcgtcgt
     2581 tctccgagtc ctcgaccagg agcacgcgca gcggggcgcc tggctccctc accagacgtc
     2641 cgccttcctc ggcgagccct cctggcggcc gcccagcgtg aaatagaagg tggctccctc
     2701 tccaatctgg ctctcggccc acaccctccc cccgtggcgg tgcacgatcc tggccaccgt
     2761 cgcgagcccg atgccggtcc cctcgaactc ttcggagccg tgcagcctct ggaacgcgcc
     2821 gaagagcttg tctgcgtacc tggggtcgaa ccctgccccg tcatcgcgca cgtaataagc
     2881 cacgctcccc ccgccgtgcg ggacgtcgac gccgaactct atggttgcct catccctctt
     2941 ggaggtgaat ttccaggcgt tgccgagcag gttctccagc gccaccgcca gcaggttggc
     3001 gtctccgaac gccacgacgc cttcctgggc gacgaacttc acccgccttt cgggatgcgc
     3061 cctctcgagc ttcttgacga tatccttcgc cattgcgctc aggtcgatca cctccctgcg
     3121 cagcggtccc cggcttaccc tggagaggtc gagcaagtcc tctatgagga gggccatgtg
     3181 cttgctggca ccccgcaccc tgcccaggta gtctgctcct tcttcgtcga gcttgtcggc
     3241 gtagtcctcc agaagtatcc gggagaagcc gtctatgctc ctcaagggcg acctcaggtc
     3301 gtgggagacc gaataggaga aggcctcgag ctccctgttg gccccctcga gctccctgtt
     3361 ggcgtcctcg aggtccttgc tcgcacgctc ggccagggtg ctgttgcgca cgagcaacag
     3421 ggtgatcccg aatagcagga ggctcaccgc gacgccgctc gcgagcacga agccgggcag
     3481 gttggagccg gcctctttct cgaaccctgg cagcgtggcg aaatacaggc tccactcgcg
     3541 accggcgacc tccatctgcc tctcctcgga gaagagggtc tctacctcgg gcttgcctgc
     3601 ccgctgcacg ccgtcgctgt cgtagaggcg ctcgctcgac cccacctccc ggctgtcata
     3661 tagctcgaag tcaatagcag ggtcgaagcc tcccccgaag acgccatcga gcaacccgtc
     3721 ccacctgaag aagccaacga cgaaaccctt cagcgcgcgc cggcgctcgg caacggtatc
     3781 ctgc
//
LOCUS       DASTUV010000027         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_389732, whole genome shotgun sequence.
ACCESSION   DASTUV010000027 DASTUV010000000
VERSION     DASTUV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389732"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFT03_00495"
     CDS             complement(<1..341)
                     /locus_tag="VFT03_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU4846648.1"
                     /translation="MDARKAVSKDGLARRLQEARERTRWLLRTVSDEDLARQHDEIMS
                     PLIWDYGHIGNYEELWLLEQAFDRGLSDRGLYDMYDASLTPRSERLRLSGHDRGRLGV
                     DGERVSCLSRLR"
     gene            complement(385..1344)
                     /locus_tag="VFT03_00500"
     CDS             complement(385..1344)
                     /locus_tag="VFT03_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4846649.1"
                     /translation="MRAMITPQFGPPDLFEERDTERPQPGPGEVLVRVVAAGTNPVDA
                     KFRSSGDAIGLEAPIILGSDVSGVVEEVGAGVTDLAPGDEVYYTPEVAGPRSNGGYAE
                     YHVVPADIVARKPASLSHEEAAAVPLAGGTAYEAIVRRLDVKVGETALVHGGAGGVGS
                     FAVQIAKAAGARVLATAGPDNQKTLASLGADVTIDYTRQDVAEVALEDTEGAGVDAVL
                     DTVGGETVVKSIPATKPFGRLATILGAQGDLTLLYRKNQALYGVFLTRERARLDELTR
                     LIERGLVRPLVEEVLDLREVGRAHERLDSGHGRGKVVLRVAEG"
     gene            1470..2012
                     /locus_tag="VFT03_00505"
     CDS             1470..2012
                     /locus_tag="VFT03_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846650.1"
                     /translation="MSEKERPEAARDLTEAFGEPYRRVMQTAVDAQQRNVQLAQGWVE
                     GLTGVLESQAETNRALTRAMESYVNVIEEAVQSQERTSRALTESLDAYRDVIERSTSL
                     QEKNTNLIESFFGDVTGELREGMQNSQAVAQGMMEGSQKQMEAFQKLLAEAMDSYMNL
                     LNAPFALYQKNLEALGRQGE"
     gene            2111..>3549
                     /locus_tag="VFT03_00510"
     CDS             2111..>3549
                     /locus_tag="VFT03_00510"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU4846651.1"
                     /translation="MQLSERNLEVRNLIGGAWEERDGRDTEPVYDPATGEVIAETPLS
                     TAEDVDRAVKKAQEAFPGWSSTPVVERARLLFRFKALLEEHFEELRDLVTLENGKDAK
                     DAGGEVRRGIEVVEFACGMPTLLMGETVRDVARGIDNASYRFPLGVVAAITPFNFPCM
                     VPLWTLPIAIGAGNTYILKPSERTPLSAVRLGELLGEAGLPDGVFSIVNGAREAVDAI
                     LEHPGIKAVSFVGSQPVAEHVYIHGAAHKKRVQALAGAKNSMIAMPDAVLDKAVPNII
                     SSAYGNAGERCLAGSVLVAVGSGQQQDRLVEAVREAASAMKVGPGYEDGELTPVIRDS
                     HRQKVRSYVDLGEEEGAKVVLDGREPPREEGFFFGPTILDNVSGDMRVAREEIFGPVL
                     SVVRVGSLDEAIEFTNTSPFGNACSIYTESGAAVRHWRENVEAGMLGVNVGVAAPMAF
                     FPFNGVKNSFYGDLHATGKDGVRFFTENKV"
BASE COUNT          674 a         1114 c         1176 g          585 t
ORIGIN      
        1 cgaagcctgg ataggcagga aactcgctcg ccgtccactc ccagacgtcc ccgatcatgc
       61 ccagagagcc gtaggcgctc gctgcggggt gtgagggagg cgtcgtacat gtcgtagagc
      121 ccccggtcag agagaccgcg gtcgaaagcc tgttccagga gccacaactc ctcgtagttg
      181 ccgatgtggc cgtagtccca tatcaggggg ctcatgatct cgtcgtgctg gcgagccagg
      241 tcctcgtcag agacggtgcg cagcagccac cgcgtcctct ctctcgcttc ttgcagtcgc
      301 cgggcaaggc catccttgga tacagctttt cgagcgtcca tgacacgaag ggtacagacc
      361 catcctgtca aatcaagacg acctttaacc ttcggccacc cgcagcacga ctttgccgcg
      421 tccgtggccg gagtcgagcc tctcgtgcgc gcggccgacc tcgcgcaggt ccagcacctc
      481 ctccaccagc gggcgcacca ggccccgttc gatcaggcgc gtcagctcgt cgagtcgtgc
      541 ccgctcgcgt gtgaggaaca cgccgtagag agcctggttc ttccggtaga gaagcgtgag
      601 gtccccctgc gcccccagga tcgtcgcgag ccgtccgaaa ggtttcgtcg ccgggatgct
      661 cttcaccacc gtctcaccgc ccacggtgtc cagaaccgcg tccacgccag ctccttcggt
      721 gtcctcgagg gcgacctcag ccacatcctg ccgcgtgtag tctatggtca cgtcggcgcc
      781 tagagacgca agcgtcttct ggttgtcggg gccggccgtg gcgaggacgc gggcgcccgc
      841 ggctttggcg atctggacgg cgaaggatcc cacgcccccg gcaccgccat gaacaagtgc
      901 cgtctcgccg accttgacgt ccagccgccg cacgatggcc tcgtaggccg tacccccggc
      961 tagcggtacg gcggctgcct cctcatgcga gagcgaagcg ggtttcctcg ccacgatgtc
     1021 cgccggcaca acgtggtact cggcgtagcc gccgttcgag cgcgggccgg cgacctccgg
     1081 cgtgtagtac acctcgtcgc ccggcgcgag gtccgtgacg cctgctccga cctcctcgac
     1141 gacccccgag acgtccgagc cgaggatgat cggggcctcg agaccaatag cgtcgccgct
     1201 ggaccggaat ttggcgtcta cgggattggt acccgcggcg acgacccgca ccagtacctc
     1261 tcccggtcct ggctgcggcc tctcggtgtc acgctcctcg aagagatccg gcggcccgaa
     1321 ctgcggcgtg atcatggccc gcatgcgatg tcctttcctc gaacctgtag cgtagttaac
     1381 tagttgcccg actcgccgcg cccgcaaacg gacgccccga gcggttagaa tgtctcctgt
     1441 gacgaacagc gggatcagga ggaagccgta tgtctgagaa ggagcgaccg gaggcggcgc
     1501 gggacctgac cgaggcgttc ggcgagcctt acaggagagt aatgcagacc gccgttgacg
     1561 cccagcagag gaacgtacag ctcgcccagg ggtgggtcga gggtctgact ggggtactcg
     1621 agagccaggc cgagacgaac cgggcgctca ccagggcgat ggagtcctac gtgaacgtca
     1681 tcgaggaggc cgtccagagc caggagcgga ccagccgcgc cctcaccgag agcctggatg
     1741 cctacaggga cgtcatcgag cgatccacga gcctgcagga gaagaacacg aacctgatcg
     1801 agagtttctt cggcgacgta acgggggagc tacgagaagg tatgcagaac agccaggccg
     1861 tggcccaggg aatgatggag ggctcgcaga agcagatgga ggccttccag aagcttctgg
     1921 ccgaggcgat ggactcgtat atgaacctcc tgaacgcgcc tttcgccctc taccagaaga
     1981 acctggaggc gctcgggcgc cagggcgaat agacccgaca ccaaccgggt atatccatgt
     2041 atgcaacgaa tagtgcgtcg ggcgggaagc ccggacgcgg tgtgtgcatg tctgaaagag
     2101 gaggttcacg atgcaattga gcgagagaaa cctggaggtc aggaacctga tagggggcgc
     2161 ctgggaggag cgtgacggac gcgataccga gccggtctac gatcccgcca cgggcgaggt
     2221 catagcagag acacccctct ccactgcaga ggatgtcgat cgggccgtga agaaggccca
     2281 ggaggcgttc cccggatggt cctcgactcc ggtagtggag cgcgcacggc tcctgttccg
     2341 cttcaaggcg ctcctcgaag agcatttcga ggagctcagg gacctcgtca ccctcgagaa
     2401 cggcaaggac gctaaggacg cgggcggtga ggtcaggcgc ggcatagagg tcgtcgagtt
     2461 cgcctgcggg atgcccaccc tattgatggg cgagacagtg agggacgttg ccagggggat
     2521 agacaacgct tcctatcggt tcccgctcgg ggtagttgcg gccataacgc ccttcaactt
     2581 cccgtgcatg gtgccgctct ggacgctgcc catagcgatt ggtgccggca acacctacat
     2641 cctgaagccc tcggagagga cccccctctc ggcggtgagg ctcggggagt tgctcggaga
     2701 agcgggtctg ccggacggcg tcttctcgat agtcaacggg gcccgcgagg ccgtcgacgc
     2761 catcctggag caccccggca taaaggccgt ctcatttgta gggagccagc cggtagccga
     2821 gcacgtctac atacacggag ccgcgcacaa aaagcgcgtt caggcgcttg ccggggcgaa
     2881 gaactccatg atagcgatgc ccgacgccgt gctcgataaa gcggtgccca acatcatctc
     2941 cagcgcttac ggcaatgcgg gagagaggtg tctcgccggg agcgtcctcg tagcggtggg
     3001 atccgggcaa cagcaggacc gcctcgtcga agccgtcagg gaggcggcct ccgcgatgaa
     3061 ggtggggcct ggctacgagg acggagagct cacccccgtg atccgcgatt cccaccgcca
     3121 gaaggtgcgc tcctacgtgg acctgggcga agaggagggg gcgaaggtcg tcctcgatgg
     3181 tagagagccg ccaagggagg aaggtttctt cttcgggcca accatcctcg ataacgtaag
     3241 cggggacatg cgggtagcaa gagaggaaat cttcggtccg gtgctctctg ttgtcagggt
     3301 tggctccctg gacgaggcca tagagttcac caacacctct ccattcggca acgcctgctc
     3361 gatctacacc gagagcgggg ctgcggtcag gcactggcgc gagaacgtag aagcggggat
     3421 gctgggggtg aatgtcggcg tcgccgcccc gatggcgttc ttcccgttta acggggtgaa
     3481 gaattccttc tacggggacc tgcacgcaac cggcaaggac ggggtgcgct tcttcaccga
     3541 gaacaaggt
//
LOCUS       DASTUV010000028         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1569575, whole genome shotgun sequence.
ACCESSION   DASTUV010000028 DASTUV010000000
VERSION     DASTUV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569575"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFT03_00515"
     CDS             <1..180
                     /locus_tag="VFT03_00515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU4846652.1"
                     /translation="TIELKTNFLGTKLAGVITCEARLAHGGRTTQIWDATVTDDDSGK
                     LLALFRCTQLILYPR"
     gene            complement(196..1077)
                     /locus_tag="VFT03_00520"
     CDS             complement(196..1077)
                     /locus_tag="VFT03_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4846653.1"
                     /translation="MGTRDLGALLLLSALWGASFIYIRVAVPALGPFVLVELRVGLAA
                     VVLALCAALVGRLPKLRRRWRQFVLLGAVNVAIPFSLISTAEINLTASLAAILNSTTV
                     MFTAVVAAVWMGDALTARKLVGVALGIAGVTVLVGWDPIAMNWVVALSVGAMLAASLA
                     YALGSVYAKRTFADSPPLAIASGQLTAAAMLMLPLAAVSAPEQSPSTIVVLSVLGLAL
                     PSTAVAYMLYFRLIANVGPTSTSTVTLLVPLFGLLFGVVLLDEPVGVGTLAGLVLILS
                     SVTLITGLGATRPKARL"
     gene            complement(1078..1872)
                     /locus_tag="VFT03_00525"
     CDS             complement(1078..1872)
                     /locus_tag="VFT03_00525"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU4846654.1"
                     /translation="MNERFTERDGELVTASLRETGARIVPARELAGLVAGVVAGVTDY
                     LFGVESPLGAVYVAAGPDGVRYLAPASSEEEFARRYRERFGRFVSPAQGDEVAGLAER
                     TAAALAGERVEVPLDLSRATPFQRRVLEVVRGISRGEVRPYVWVAGEAGSPGASRAVG
                     NVMANNPVPLLVPCHRVVRNDGSTGSYAFGAGEKVRLLRAEGVSPEEISGSPYVATPT
                     TGIVCHATCRNAKRIQPENRRTFRSVREATAAGYRPCKVCRPVVAA"
     gene            complement(1869..2438)
                     /locus_tag="VFT03_00530"
     CDS             complement(1869..2438)
                     /locus_tag="VFT03_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4846655.1"
                     /translation="MKTRARPQEWATGLDPLAARLADGDPLAPKALVERHYRELYRYA
                     FSVLREERSAEDAVQDAFERAFSALGRYSEERLRAMRLRPWMFRITLNVARNRLRQRR
                     EVPVEDLSVEAASDEDRESIMDTLAALGGLPERQRVAVTLRYLQDMPYAEISSVTGWP
                     EGTAKTLVRRGLMRLRSLMVEEPKGGGQG"
     gene            complement(2454..2789)
                     /locus_tag="VFT03_00535"
     CDS             complement(2454..2789)
                     /locus_tag="VFT03_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU4846656.1"
                     /translation="MIARIWHGITAAAKSDEYLDYLEQTGVPDYRATQGNRGVYVLRR
                     AEGEQAHFLTVSFWESMEAIKDFAGPDPERARYYPEDEEYLLGFEPTVEHFEVLAGRV
                     SDVRNFPGR"
     gene            complement(2814..3122)
                     /locus_tag="VFT03_00540"
     CDS             complement(2814..3122)
                     /locus_tag="VFT03_00540"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU4846657.1"
                     /translation="MTTLWISIAAVALASATIKAAGPVLVGGRELPQRAVSVIALLAP
                     ALLAALVVTQTFGGDRHLVLDERAVGVGVAAVALTLRAPVLLAVALAAVSTALVRAFV
                     "
     gene            complement(3119..>3510)
                     /locus_tag="VFT03_00545"
     CDS             complement(3119..>3510)
                     /locus_tag="VFT03_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846658.1"
                     /translation="PHLKGGPFRRALEGQAVVDASWALASRGEGRFDREFMIGATVPQ
                     GVAWIAGTAIGAVGGDFIGDPERLGLDVIFPAFFLALLAEELRRGRAVAVAVIAAALA
                     LALVPFAPPGVPVIAACAAALLGLRRS"
BASE COUNT          628 a         1262 c         1106 g          514 t
ORIGIN      
        1 accatcgagc taaagaccaa tttcctcggc accaaactcg ccggcgtcat aacctgcgag
       61 gcacgcctcg cccacggcgg ccgcacgacc cagatctggg acgcaaccgt caccgacgac
      121 gatagcggca agctcctggc ccttttccgc tgcacgcagc tgatcctcta cccccgctga
      181 cccgaggcga tattctcaca accgggcctt cggccgcgtt gcgccgagcc cggttatgag
      241 ggttacgctc gagaggatga ggacgaggcc agcgagcgtc cccaccccta cgggctcgtc
      301 gaggagtact acaccgaaca ggagcccgaa gagcggcacg aggagcgtga ctgttgaggt
      361 gctggtagga ccgacgttcg cgatcaggcg gaagtagagc atataagcca cggccgtcga
      421 gggcagagcg agcccgagaa ccgagagcac cacgatcgta gacgggcttt gctctggagc
      481 gctcacggcg gcaaggggca gcatgagcat agcggccgca gtcagctggc cgctggcgat
      541 cgccagcggc ggcgagtccg cgaacgtccg cttagcgtag accgagccaa gagcgtacgc
      601 gagcgaggcg gcgagcatcg ccccgactga gagcgcgacg acccagttca tagcgatggg
      661 atcccagccg acgagaaccg tcaccccggc tatcccgagc gccacgccga cgagcttcct
      721 cgccgtcagg gcgtcgccca tccacaccgc cgccaccacc gctgtgaaca tcacggtcgt
      781 ggagttcagg atggccgcaa gagaggccgt caggtttatc tccgcggtgc ttatgaggga
      841 gaagggtatc gccacgttca cggctccgag gagcacgaac tgcctccagc gcctccgcag
      901 cttcggcaga cgccccacga gcgccgcgca gagtgcgagt accacggcgg cgagccccac
      961 gcgcagctcc acgaggacga aaggcccgag agctggcacg gcgacccgga tatagatgaa
     1021 ggacgcgccc cagagcgcgc tgaggaggag cagcgctccc aggtcgcgcg tacccatcta
     1081 agcagccacc acgggtcggc acaccttgca gggccggtaa cccgccgcgg tagcctccct
     1141 gacgctgcgg aaggtgcggc ggttctcagg ctgtatcctc ttcgcgttgc ggcaggtagc
     1201 gtggcatacg atgccggtcg tcggcgtcgc gacgtagggc gagccggaga tctcctccgg
     1261 cgatacaccc tcggctctca acaacctcac cttctccccc gcaccgaacg catagctccc
     1321 ggtactcccg tcgttgcgga cgacccggtg acaggggacg agcagcggca cggggttgtt
     1381 cgccatgacg ttgccgacgg cccgcgatgc gcctggagaa cccgcctcgc cggcaaccca
     1441 gacgtagggg cgcacctcgc cgcggcttat gcccctcacg acctcgagca cccgccgctg
     1501 gaacggcgta gcccggctca ggtccagcgg cacctctacc cgctccccgg ctagcgcagc
     1561 cgcggtcctc tccgcgagcc ccgctacctc atccccctga gccggcgaga cgaaccgccc
     1621 aaagcgctcg cggtagcggc gggcgaactc ctcctcggaa gatgcaggtg caaggtaccg
     1681 gacgccgtca ggccccgccg ccacgtacac cgccccgagc ggcgactcca cgccgaagag
     1741 gtagtcagtt accccggcca ccacgcctgc taccagaccg gccagctctc ttgccggcac
     1801 gatgcgtgcc cccgtctcgc gcagcgacgc ggtcacgagt tcgccgtcgc gctccgtgaa
     1861 tctctcgttc atccctgacc accacctttt ggctcctcga ccatcaaact ccgtaggcgc
     1921 atcaggcccc ttcggaccag cgttttggcg gttccctcgg gccacccggt tacgctggag
     1981 atctccgcgt acggcatgtc ctgcaagtag cgtagcgtca ccgccaccct ctgtctctcg
     2041 ggcagccctc ccagcgcggc cagcgtatcc atgatgctct ccctatcctc gtcgctcgcg
     2101 gcctcgacgg agagatcctc gaccgggacc tcgcgtctct gccggagtcg gttgcgagcg
     2161 acgttcaggg tgatcctgaa catccacgga cgcagcctca tggcacggag cctctcctcg
     2221 gagtaccgtc cgagcgccga gaaggcacgc tcgaaagcgt cctgcacggc atcctcggcg
     2281 gaccgctcct cgcgcagcac cgagaaggcg tagcggtaca gctcgcggta gtgccgctct
     2341 accagggctt tgggcgccag agggtcgccg tccgccagtc gcgccgcgag cgggtcgagt
     2401 cccgtcgccc attcctgtgg ccgcgccctc gtcttcatac caggtacaac aatctagcgg
     2461 ccaggaaagt ttcgtacgtc gctcacgcgt cctgccagga cctcgaagtg ctcgacggtc
     2521 ggctcgaagc cgagcagata ttcttcgtct tccggatagt atctggctct ctccgggtca
     2581 ggacctgcga aatcctttat cgcctccatt gattcccaaa aagacaccgt caggaaatgc
     2641 gcctgttcgc cctcggccct gcggaggacg taaacgcccc ggttcccctg cgtcgcccgg
     2701 taatcgggga cgccggtctg ctcgaggtag tcgaggtact catcggactt tgcggctgcg
     2761 gttatgccgt gccagatcct cgcgatcatg gttcctccct gatatctctc gtgtcagaca
     2821 aaggctcgaa ccagcgcggt gctaacggcg gcgagggcga cggcgagcag cacgggcgcc
     2881 cgtagcgtga gagctacggc ggcgacgccc acccccacgg ctctctcgtc gagcacgagg
     2941 tgccggtcac caccgaaggt ctgtgtcacc acgagggccg caaggagcgc gggcgcgagc
     3001 agagcgatga ccgaaaccgc gcgttgaggc agctcccgtc cgccgaccag tacgggtccg
     3061 gcggccttga tggtcgcgct ggccagggcg acggcggcga tcgagatcca gagcgtcgtc
     3121 atgaccgcct cagcccgaga agagccgcag cgcaggcggc tatcaccggc accccagggg
     3181 gagcgaacgg cacgagagcg agggccagcg cggcggctat gaccgccacc gcgaccgccc
     3241 gcccgcggcg cagctcctcc gcgagcaggg cgaggaagaa cgcggggaag atgacatcca
     3301 gccccagccg ctcggggtcg ccgatgaaat ctccgcccac agccccgatg gcggtgccgg
     3361 cgatccaggc tacaccctgc ggcaccgtgg cgccgatcat gaactcccgg tcgaagcggc
     3421 cctcgccacg actggccagc gcccacgacg cgtcgactac cgcctgcccc tccagagcgc
     3481 gcctgaaggg cccacctttg aggtgagggg
//
LOCUS       DASTUV010000029         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1356077, whole genome shotgun sequence.
ACCESSION   DASTUV010000029 DASTUV010000000
VERSION     DASTUV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356077"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1364
                     /locus_tag="VFT03_00550"
     CDS             207..1364
                     /locus_tag="VFT03_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU4846659.1"
                     /translation="MMHKYRLMSPGPTPIPPEVSAAGALPIIHHRTPEFGEVFTRVNE
                     NLKKVFLTGNDIFTYAASGTGAFEGAIQNLFSPGEKVLVVNNGNFGNRWVKMSRAFGL
                     EVTELKYDWGAKADNDEVAGALAHDPEIKAAICVLSETSTGTVNDIEGFARAAENVIS
                     IVDAVSGLGACPLRTDEWGVDVVVAGSQKALMTPPGLGFVSVSERAMRAHEESTMPRF
                     YFDWTAAKKAYAKEPAQTAWTPAVSLIIQLDVALLQLLEEGVEHVFERHVLLGRAARA
                     GIKGMGLNLFGPDEDLNSAVTAAWVPEGIDGKQFVKMLFREHGIQVAGGQGEMAGKIF
                     RIGHCGYFDAYDIIATIAAAELALESLDYPVELGRGVGAAQRVFSKSGVAA"
     gene            1361..2941
                     /gene="serA"
                     /locus_tag="VFT03_00555"
     CDS             1361..2941
                     /gene="serA"
                     /locus_tag="VFT03_00555"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEU4846660.1"
                     /translation="MKVLITEKIADRGIELLTEEFEVDVLLGLSPEELLEKIGDYHGL
                     IIRSATKVTAEVIERAQNLKAIGRAGIGVDNIDIEAATKRGVLVANAPESNTVAAAEH
                     TLGLMLAAARRIPAADSSLREGEWKRSAFKGVEVSEKTLGLVGLGHVGAIVARGAAGM
                     GMRVLAYDPYVSEDRMRSMNVERAESLDEVFESSDFISLHVPRTPQTAGMVDAGALEK
                     MKPTAYLINVARGGIVDEMALYNALKDRTIAGAALDVFATEPNTDSPLFSLPNVVVTP
                     HLGASTAEAQDRAGVTAAEQVAIALRGAVPMHAINAPVPVGEGAEFVAQFSGLCEALG
                     SLLYQLTDRPGNVLRIEYTGEIAGFDTRLLDVSVQKGLLAPMVFEPLNYVNTPALARD
                     RGLKVETSRVSESADYTSLVVLRLEDDRGENVVSGTLVGPRMQPRLVEVMGFDMDIVP
                     EKHMLFIRNEDVPGMIGKLGSILGEHGINIGNMAVGRGEPGSRAAMAVTVDEPVPEEV
                     LKVLLNTPGFNQARAVSL"
     gene            2941..3153
                     /locus_tag="VFT03_00560"
     CDS             2941..3153
                     /locus_tag="VFT03_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEU4846661.1"
                     /translation="MSSLEDILDRYRPYGPTYRPGDKAPFSGTYVCDRGTMLPPVRVQ
                     LSRGEEFPEAEGLGPHTRWRGTNIQA"
     gene            3238..3876
                     /locus_tag="VFT03_00565"
     CDS             3238..3876
                     /locus_tag="VFT03_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEU4846662.1"
                     /translation="MGKKGGGLGTTLVLALVIVVVGVGLGVALSTQGFMERLPVIGPL
                     FYEEEPAQTTTGPVVVEGVQDLNRLATVRWRESVIVTRESGGTELERFLAGEKVLLVA
                     TGDVEAGVDLASLGRDDVKVNGETVTISLPEPEILSVSLDEQKTGVYDRDFGPLNLHP
                     DDDLVEQARAAAVDRIEQAARDEDILDQAEQNAEDSIRAFVRTLGFEEVRFE"
     gene            complement(3973..>4178)
                     /locus_tag="VFT03_00570"
     CDS             complement(3973..>4178)
                     /locus_tag="VFT03_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEU4846663.1"
                     /translation="GVTLTESCAMSPPSAVSGYYFSHPESTYFGVAEIGRDQAQEYAA
                     RKGMTLEEAERWLSPNLGYDPKG"
BASE COUNT          792 a         1267 c         1424 g          695 t
ORIGIN      
        1 gcaccaaggt cctcttcgga cagaacctga tccacgatga gacaggcatc ctgcgtacgg
       61 gcgaccccgt ggagctgatc acacgctctt cgccaggcta aaagcaacac tttgtaaatt
      121 agctgcgtca ttatgtagcc atcagcagcg atagttaata gaataggcgt gagttgtccg
      181 ctgcaagaga ggaagcctcc caacctatga tgcacaaata ccgtcttatg tcgcctggtc
      241 cgactcccat accgccggag gtctcggcag ctggagcgct acccataatc caccaccgga
      301 cgccggagtt cggcgaggtc ttcacacggg tcaacgagaa cctgaagaag gtctttctga
      361 ccgggaacga catcttcacc tacgcggcga gcgggactgg ggcattcgag ggggcgatcc
      421 agaacctctt ctctccgggc gagaaggtcc tggtggtcaa taacggcaac ttcggcaacc
      481 gctgggtcaa gatgagccgg gccttcggcc tcgaggtgac agagctgaag tacgactggg
      541 gcgcgaaggc ggacaacgac gaggtcgccg gggcgctggc ccatgacccg gagatcaagg
      601 ccgccatttg cgtcctctcc gagacctcga cgggcaccgt caacgacatc gagggcttcg
      661 cccgcgcggc tgagaacgtg atatccatag ttgacgcggt ctccgggctc ggggcgtgcc
      721 ctttgaggac ggacgagtgg ggagtggacg ttgtcgtcgc ggggagccag aaggcgctca
      781 tgacgccgcc ggggctaggc ttcgtcagcg tgagcgagag ggcgatgcgg gcgcacgagg
      841 agtcgactat gcctcgcttc tacttcgact ggacggcggc gaagaaggcg tatgccaagg
      901 agccggccca gacggcctgg acgcctgctg tcagcctcat catccagctc gacgtcgcgc
      961 tgctgcagct cctcgaggaa ggcgtcgagc acgtcttcga gcgccacgtc ctgctcggcc
     1021 gtgccgcgcg cgctggcatt aagggcatgg gcctcaacct gttcgggccg gacgaagacc
     1081 tgaactcggc tgtgacggct gcctgggttc ccgaagggat agacggcaag cagttcgtca
     1141 agatgctctt cagggagcac gggatacagg tcgctggcgg gcagggcgag atggcgggaa
     1201 agatcttccg catcggccac tgcggctact tcgacgccta cgacatcatc gccacgatag
     1261 cagccgccga gctcgcgctg gagtcgctcg attaccccgt agagcttggc aggggcgtcg
     1321 gcgccgccca gcgcgtcttc tcgaagagcg gggtggcagc ttgaaggtac tcataaccga
     1381 gaagatcgcc gaccgcggca tcgaactgct gacagaggag ttcgaggtgg acgttctgct
     1441 cgggctcagc cccgaggagc ttctggagaa gatcggggat taccacggcc tcataatccg
     1501 cagcgcgacg aaggtcaccg ccgaggtcat agagcgggcg cagaacctca aggccatcgg
     1561 gcgggccggc atcggggtgg acaacataga catagaggcg gctaccaagc gcggcgtcct
     1621 ggtagccaac gcgcccgaga gcaacacggt agccgccgcc gagcacactc tgggtcttat
     1681 gctcgccgca gcgcggcgca tcccggccgc tgacagctct ctgcgggagg gtgaatggaa
     1741 gcggagcgcc ttcaagggcg tcgaggtctc ggagaagacg ctgggtctcg ttggcctggg
     1801 tcacgtcggg gccatcgtcg cgcggggtgc tgcggggatg ggtatgcgcg tgctcgcata
     1861 cgacccttac gtctcggagg acaggatgcg ttcgatgaac gtggaaaggg ccgagagcct
     1921 ggacgaggtc ttcgagagct cggacttcat ctcgctgcac gtgccccgca ccccgcagac
     1981 cgcgggcatg gttgacgcgg gtgccctgga gaagatgaag ccaacagcct acctcataaa
     2041 cgtcgcgcgc ggcggcatcg tggacgagat ggccctgtac aacgcgctca aggacaggac
     2101 catcgcgggc gccgcgctcg acgtgttcgc cacggaaccg aataccgatt cgccgctctt
     2161 ctcgctcccg aacgtcgtgg tgacgccgca cctcggggcg agcacggccg aggcgcagga
     2221 ccgggccggc gtcaccgcgg ccgagcaggt cgcaatagcg cttcggggtg cggtgccgat
     2281 gcacgccata aacgctcctg tccctgtcgg cgagggggcc gagttcgtcg cgcagttctc
     2341 gggcctctgc gaggcacttg ggagcctcct ctaccagctc accgacaggc cggggaacgt
     2401 actcaggatc gagtacacgg gtgagatcgc cggcttcgac actcgcctcc tcgacgtctc
     2461 ggtccagaag ggcctcctcg cgccgatggt cttcgagccc ctcaactacg tcaacacacc
     2521 agctctcgcg cgagacaggg gtctcaaggt ggagacctcg cgggtctccg agagcgccga
     2581 ctacaccagc ctcgtcgtac tcaggctcga ggacgacagg ggggagaacg tggtgagcgg
     2641 gacgctggtg ggaccccgga tgcagccccg gctcgtcgag gtcatgggct tcgacatgga
     2701 catcgtgccc gagaagcaca tgctcttcat ccgcaatgag gacgtgccag gcatgatcgg
     2761 gaagctcggt agcatcctgg gcgagcacgg catcaacatc gggaacatgg ccgtggggcg
     2821 tggcgagccc ggctcccgcg cggcgatggc cgtgaccgtg gacgagccag tccccgaaga
     2881 ggtcctcaaa gtcctgctga atactccagg cttcaaccag gcccgggctg tgtcgctgtg
     2941 atgtcctccc tggaggacat actcgaccgc tacaggccct acggcccgac ctacaggccc
     3001 ggcgacaagg cgcccttctc cggcacctac gtgtgcgaca ggggtaccat gctccccccc
     3061 gtgcgcgtcc agctctccag aggcgaagag ttccctgagg cagagggcct gggaccacac
     3121 acccgatggc ggggcactaa catacaggcg taggcttcag gtgaaagtaa gctttgcacc
     3181 ttagtggtcc gtcggatgct agaatcttgc ctctaggacc ttcgtaggag gcatgaggtg
     3241 ggcaaaaaag gtggcggact cgggacgacg ctagttctgg ctctcgttat cgtcgtggtt
     3301 ggcgttggac tgggcgtcgc tctcagcacc cagggcttca tggagcgctt gcccgttata
     3361 ggacctttgt tctacgagga ggagccagct cagaccacaa cggggccggt cgtggttgag
     3421 ggtgtccagg acctcaaccg gctcgcgacc gtacgctgga gggagtccgt gatcgtcacc
     3481 agggagagcg gcggaacgga gctggagagg ttcctggccg gggagaaggt cttgctcgtc
     3541 gcgaccgggg acgtggaggc cggcgtggac ctggcttcgc tgggtcgcga cgacgtgaag
     3601 gtgaacggag agacggtaac gattagtctt ccggagcccg agatcctctc cgtcagcctc
     3661 gacgagcaga agaccggcgt ctacgatcgg gacttcggcc cgctgaacct ccaccccgac
     3721 gacgatctgg tcgagcaggc ccgcgccgct gcggtggaca ggatagagca ggccgcccgc
     3781 gacgaggata tcctggatca ggccgagcag aacgccgagg atagcatacg ggctttcgtc
     3841 aggactctgg gtttcgagga ggtgcggttc gagtagaccc gccgctccca gcaagggtac
     3901 ctggaggagt gcgccgaacg ggcgagccgg cgtcgagggg cgaccagggc gagatcaaaa
     3961 cctgtactct gactatcctt tggggtcgta gccgaggttc ggggagagcc acctctcggc
     4021 ttcttcgagc gtcatgccct tgcgcgcggc gtactcttga gcctgatccc gccctatctc
     4081 ggcgacgccg aagtaggtgg actcggggtg ggagaaatag tagccgctga cagccgaggg
     4141 cggcgacatc gcgcagctct cggtgagggt gacgcctg
//
LOCUS       DASTUV010000030         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_854146, whole genome shotgun sequence.
ACCESSION   DASTUV010000030 DASTUV010000000
VERSION     DASTUV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854146"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFT03_00575"
     CDS             complement(<1..581)
                     /locus_tag="VFT03_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU4846664.1"
                     /translation="MATKRDYYEILGVSREASDAEIKKSYRRLARSHHPDANPGDHEA
                     EERFKELTEAYEVLSNPEARRAYDTYGHQVPRGAGAAGYPGGGDPFGGFQDIFEAFFG
                     DRSFGGSFFGTSTAQGPSRGSDAEVEVEVSLYEAAFGTDREVNVQVVRNCGVCDGIGG
                     TESHVCPTCAGAGSIRTVRESILGQMVTTQACST"
     gene            complement(582..1610)
                     /gene="hrcA"
                     /locus_tag="VFT03_00580"
     CDS             complement(582..1610)
                     /gene="hrcA"
                     /locus_tag="VFT03_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU4846665.1"
                     /translation="MRHLELSERQRELLLKTLEIYISTGAPVGSHALSEELDASSSTI
                     RAELAHLESLGLLDHPHTSAGRVPTDSGYRYYVDALMEANSREETAFAALEGYGNVDE
                     LLRGVSEGMSEVTSLLAVVAGPTVLGESPARVDFLPLGEGRLLLVVSMESGASSSAAI
                     TLPIPVEDDELRHIFAALNSWLGGRPLGTDLELGLAARTALSDHDPMTVGAVLEAVGA
                     LGRATERGVFIQGASALLSRLDDLDPGSLSAVIEVFERRRWLLRLMGDALRRSVSSSG
                     VVVSIGTESGFLNLTGTSLVASAYSHHERPYGVVSLIGPKRMDYDVAITTVRSAAEAL
                     TNCLDARF"
     gene            complement(1747..>2290)
                     /locus_tag="VFT03_00585"
     CDS             complement(1747..>2290)
                     /locus_tag="VFT03_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564495.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU4846666.1"
                     /translation="GAVMGLCHERRGVSVGMEYISTRRVQLTYDLPLAEIITDFFDAM
                     KSRTKGYASYDYEPLGYEPSDLVKVEVLVSGDTVDALSIIVHRDKAYQRGRALVEKLK
                     ELIPRQQFEVPVQATMGKRVIARETVRAYRKDVTAKCYGGDITRKRKLLEQQKKGKRR
                     MKQVGSVEIPQEAFLAALRI"
BASE COUNT          400 a          729 c          725 g          436 t
ORIGIN      
        1 gtcgagcacg cctgcgtcgt aaccatctgc ccgaggatgc tctcgcgcac cgtcctaata
       61 gagccggcgc cagcgcaagt gggacagacg tgtgactccg ttccccctat gccgtcgcag
      121 accccacagt tcctgacaac ctgaacgttg acttcacggt cagtcccgaa cgcggcctcg
      181 tacagggaga cttctacctc gacctcagcg tcactacccc gactcgggcc ctgcgcggtg
      241 gaggtgccga agaaggagcc gccgaaagag cggtcgccga agaaagcctc gaagatgtcc
      301 tggaagcctc cgaacgggtc tcccccgccg gggtagcctg cggctcctgc gccgcgcggt
      361 acctggtggc cgtatgtatc gtaggcgcgt ctagcttccg ggttcgagag tacttcgtag
      421 gcctcggtaa gctccttgaa gcgttcttcg gcttcgtggt cgcctgggtt cgcgtcgggg
      481 tggtgggagc gggcgaggcg acggtaagat ttcttgatct ccgcgtccga agcttccctg
      541 gacacgccca gaatctcgta gtaatcgcgc ttggttgcca actagaacct ggcgtccaga
      601 cagttggtca gggcctccgc ggccgagcgg accgtcgtta tggcgacgtc gtagtccatc
      661 cgcttcgggc cgatcaggct gacaaccccg tatggccgct cgtggtggct gtaggccgag
      721 gccacgaggc tcgtgcccgt caggttcagg aacccgctct ccgtgccgat ggagacgacg
      781 actccgctgc tcgaaacgga gcgacggagg gcgtcgccca tcagacgcag caaccagcgc
      841 cggcgctcga aaacctctat cacggctgag aggctgccag ggtcgaggtc gtccaggcgg
      901 ctaaggaggg ccgaggcgcc ctggatgaag accccgcgct ccgtcgccct tcccagcgcg
      961 cctacggcct ccaggacggc tcccactgtc atagggtcgt ggtccgaaag cgccgtgcgg
     1021 gcagcgagcc cgagctcaag gtcagtccca agcggacgtc ctccaagcca ggaattgagg
     1081 gctgcgaaga tgtggcgcaa ttcatcatcc tcgacgggga tggggagtgt gatcgcggcg
     1141 ctcgaagagg cgccgctctc catcgataca acgagcagga gcctgccttc gccaagaggc
     1201 agaaagtcga cacgggcggg cgactcgccg aggacggtcg gcccggcgac cacggccaga
     1261 aggctggtca cctcgctcat accctccgag acgccacgta gcagctcgtc cacgttgccg
     1321 taaccttcga gggctgcaaa cgccgtctcc tccctcgagt tcgcttccat cagggcgtcg
     1381 acgtagtaac ggtagccact gtccgtaggg acccttccgg ccgacgtgtg cggatggtct
     1441 agcaggccca gggactcgag gtgggccagc tccgcacgga tcgtggaact gctcgcatcc
     1501 aactcctcgg acagggcgtg gctaccgaca ggggctcccg tggagatgta gatctccagc
     1561 gttttgagca gtagctcgcg ttgcctctct gaaagctcga ggtgcctcat atcgctggtg
     1621 attttaacat gggtgcggat tctcccaaat cagcatttca gcttcctgta cgcgagctct
     1681 ggttgacctg ctcaaaggcg gcggagccgc ggtgatgact agccgctgca ccttgctgag
     1741 atgctctcaa atacgcagcg ccgccaggaa ggcctcctgc ggtatctcca cgctcccgac
     1801 ctgcttcatg cggcgcttgc cctttttctg ttgctcgagc agcttgcgtt tgcgggttat
     1861 gtctccgccg tagcatttgg cagtaacgtc tttgcgatag gcgcgaacag tctcgcgtgc
     1921 gatcacgcgc ttgcccatcg tggcctgcac tggcacctcg aactgctggc gtgggatcaa
     1981 ctctttgagc ttctcgacga gtgcgcggcc gcgctggtag gccttatcgc gatggacgat
     2041 gatcgagagc gcgtccacgg tgtcaccaga gacgaggacc tcgaccttca ccaggtcgga
     2101 aggctcgtag ccaagcggct cgtagtcgta ggaggcgtag ccttttgtgc ggctcttcat
     2161 ggcgtcgaag aagtccgtga tgatctcggc cagcggcagg tcgtaggtga gctgcaccct
     2221 gcgggtcgag atgtactcca tcccgacgga gacaccgcgt ctctcgtggc atagtcccat
     2281 cacggccccc
//
LOCUS       DASTUV010000031         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_389635, whole genome shotgun sequence.
ACCESSION   DASTUV010000031 DASTUV010000000
VERSION     DASTUV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389635"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..2159
                     /locus_tag="VFT03_00590"
     CDS             93..2159
                     /locus_tag="VFT03_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU4846667.1"
                     /translation="MRVSSTKAARRMMSYPRAGLFRVALAGVTALLVSRGWALAEAWV
                     AYLALGLEAIFGALLILGRPKVLSQERLAHTVIDSALVGVLVAGTGGENSPFFLLFFL
                     AALGILRLETPAKAVAATAAVVGAYLVATAAPGDPGMLWSTSEGLRAGLLALFCAVVG
                     LWASEMHNYRRLASGLASAFAAELSHVERAEALVARFGSALEFLSVEGILQWTAEAAH
                     EVGGGSYAHVAGLNGNNHRTVVKGEFDAYPSWWHPSIQRLVLWSCREEEVVRSDEAIH
                     GIEGFVAVPIDQAGGEMWGAVVLGGKTFGAEEERALKLLAAGVARALNNAEVAPGGLD
                     QASGLPNRASLRRVLRRELSLGGSLTVLATGLRGFGAYSRIRGDAAADDLLRRLGQRL
                     GSRQRAFHRGEEEFVVVLSGSDETRARKTASAIRQLISEVAGGSEDITLTAAVGFAFA
                     RAGDEDPDVVLDAALHALEEARKRADGVAGALTIARVSGGQGSDAQLAEKAGALIKPL
                     ESRDPLIGDHLRAVSRLASRIGSRMSLPPDLLQALTFGALLHDLGKIGISDEILQKPV
                     RLTDEEYNVIKGHPVLGADMLAPIEELAPAVPVVRHHHERFDGRGYPAGLRGEEIPLA
                     ARVVSVADAFDTMTRDRPYGYGISRQAALEEVERNSGTQFDPLTVRALLEVVWGFDDQ
                     RAGSVG"
     gene            2459..3424
                     /locus_tag="VFT03_00595"
     CDS             2459..3424
                     /locus_tag="VFT03_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU4846668.1"
                     /translation="MLILRRLDGSMVGAFSARGALPEAVRKAVEESDNVGSHEGREAS
                     APARRAAMQARFFGHFELLCDGEHVHLGRNGKVLAILKYLLAHRSRPVSQDHLMGWLW
                     PDSNLKKARWSLNSAIHGLRKLLGRCPSSGGESVSYILLEEGYYRLSPAVGVATDIED
                     FDEGYERGRRLERMGRAEEAARAYEEAVALYRGDYLLEDLYEDWTMVERERLSNAHVE
                     MLVRLAVHYMQSGQLRDSVRACYEVLEKDRCHEDSHRLLMSCYVRLGQRGRALRQYRL
                     CERILGQEYGTIPSPEIQDLYRSILGDEGGARSRGGNGGSGLGAP"
     gene            3554..4018
                     /locus_tag="VFT03_00600"
     CDS             3554..4018
                     /locus_tag="VFT03_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4846669.1"
                     /translation="MSEKRTRRSSERSPLQSERGTTSISYPVVAQIAGIAAQEVEKVQ
                     MGGGATAAVGGFLQSVTGSSSGNYARGVTVEVGEEEAAVDLTMAVEYGYSVPQLTEAV
                     RRNVINRVESLTGLRVTEVNITVNDVQIPDERPMLDEQEQVERQARQQEQQA"
BASE COUNT          727 a         1211 c         1430 g          698 t
ORIGIN      
        1 gccgtcagag ccgagctcgg aagctatgga ggacgtggta cggggctttc ctcggtagtg
       61 cgtccatcgc ccatgcaaga ggaaggtttg atttgcgagt atcctctacc aaggcggcgc
      121 ggaggatgat gagctacccc agagcagggc tattccgcgt agcgcttgcc ggcgtgacgg
      181 ctctgctcgt gagccgcggg tgggctctgg ccgaggcgtg ggtcgcctac ctggcgctcg
      241 gcctcgaggc gatcttcggg gcgctcttga tcctggggcg gccgaaggtt ctctcgcaag
      301 agaggctcgc gcacaccgta atagactccg cgctggtagg cgtgctggtg gcgggcaccg
      361 gaggtgagaa ttccccgttc ttcctgctgt tcttcctggc ggcgctgggg atcttgcggc
      421 tcgagacccc cgcgaaggcc gtcgccgcga ccgccgccgt ggtcggcgcc tatcttgtag
      481 cgaccgccgc ccccggcgat cccggcatgc tctggtctac ttcagagggg ttgagggccg
      541 ggctcctcgc tctcttctgc gcggtcgtgg ggctgtgggc ctccgagatg cacaactaca
      601 ggaggctcgc ctcagggctc gcctcggcct tcgccgccga gttgagtcac gtagagaggg
      661 ccgaggcctt ggtcgcgagg ttcgggtctg cgctcgagtt ccttagcgtc gagggcatac
      721 tgcagtggac ggccgaggcg gcccacgagg tgggcggcgg gtcttacgcg cacgtcgcgg
      781 ggctgaacgg gaacaaccac cggacggtag tgaagggcga attcgacgca taccctagct
      841 ggtggcatcc ctctatccag cgcctcgtgt tgtggagctg tcgcgaggaa gaggtagtgc
      901 gcagcgacga agctatccac ggcatagagg gcttcgtggc ggtgcccata gatcaggctg
      961 ggggcgagat gtggggggcc gtggtcctgg ggggaaagac tttcggcgcc gaggaggaac
     1021 gcgccctcaa gctcctcgcc gccggggtgg cccgggcgct caacaacgcc gaggtggcac
     1081 cgggtgggct ggatcaggcc tcggggctgc cgaaccgcgc gtcgcttcgc agggttctgc
     1141 ggcgggagct ctccctcggc ggatcgctca cggtcttggc caccggacta cgaggcttcg
     1201 gagcctacag ccggatccgc ggggacgccg ctgcagacga cctcctgcgg aggttgggcc
     1261 agaggctcgg gagccggcag cgcgctttcc atcggggcga ggaagagttc gtcgtcgttc
     1321 tgagtggctc cgacgagacc agggcgcgca agacagcgtc cgcgatccgg caactgatct
     1381 ccgaagtggc cggtggatct gaagatatta ccctgaccgc tgcagtgggc ttcgcgttcg
     1441 ccagggcggg cgacgaagac ccggacgtgg tcctggacgc ggccctgcac gctctggagg
     1501 aggcaaggaa gcgagcagac ggcgtcgccg gagcgctgac catcgccagg gtctccggag
     1561 ggcagggaag cgatgctcag ctcgcggaga aggccggcgc cctgataaag cccctggagt
     1621 cccgggaccc ccttatcggg gaccatctga gggcggtttc ccgcctcgct tcacgcatcg
     1681 gctccaggat gtcgttgccc ccagatttgc tgcaggcgct caccttcgga gcgctcctgc
     1741 acgacctggg caagatcggg atttcagacg agatcctgca gaagccggtg cgcctgacag
     1801 acgaggagta caacgtgata aaggggcacc cggtcttggg agccgatatg ctcgcgccta
     1861 tcgaggagct cgcgccggct gtacccgtgg tcaggcacca tcacgagcgc ttcgacggca
     1921 ggggataccc ggccgggctg cgcggcgagg aaataccgct ggcggcgcgc gtggtctccg
     1981 tggccgacgc tttcgacacg atgacccgtg acaggcccta cgggtatggg atctcccggc
     2041 aggccgccct tgaagaggtc gagagaaact ccgggacgca gttcgatccc ctgaccgtga
     2101 gggcgctgct cgaagtggtg tgggggttcg acgaccagcg ggcgggttcc gtcggttagg
     2161 ggataacccg acgggcgctg cgtccgtggc cttcggcgaa tactcaaaaa tagtccattt
     2221 gagtgatacg ccgacccttg cccctggtat atcctgccag tagcatggtt gcgagggcga
     2281 gcccctcaat ttaattgggg aaaggagccg ctaccgtata tgggacaagg gcacacgaac
     2341 cgtcgtgcca gagataagaa tacatatcga accgtccggt ttcagtaatc gggagagaga
     2401 gatgcaagga acgtctttag agttaccggc cggatattac ctcgaacggg atccggacgt
     2461 gctgatctta cgccgcctcg acggctccat ggtcggggcc ttcagcgctc gcggcgctct
     2521 gcccgaagcc gtcaggaagg ccgtcgagga gtccgacaac gttggatcgc acgaagggcg
     2581 cgaggcttcg gcccccgcca ggcgggcagc gatgcaggcg cgtttcttcg gccacttcga
     2641 gctcctctgc gatggtgagc acgtccacct ggggcgtaac ggcaaggttc tcgccatcct
     2701 caagtacctc ctggcccacc gatcgcggcc cgtctcccag gaccacctga tgggctggct
     2761 gtggcccgat tccaacctca agaaggcgag gtggtccctg aactccgcca tccacggcct
     2821 gcgcaagctc cttggtcgct gcccctcctc aggaggagag tctgtgagct acattttgtt
     2881 ggaggagggc tactatcgcc ttagccctgc cgtaggcgta gccacagaca tcgaggactt
     2941 tgacgaaggc tacgagcgag gaaggcgcct cgagaggatg ggcagagcgg aggaggctgc
     3001 cagagcctat gaggaggccg ttgcgctcta caggggtgac tacctcctcg aagacctcta
     3061 cgaggactgg acgatggtag agcgcgagcg cctctctaac gcccacgttg agatgctagt
     3121 caggttggct gtgcactaca tgcagagtgg gcagctaagg gatagtgtca gggcctgcta
     3181 tgaggttctg gagaaagacc gctgccacga ggatagccac aggctgttga tgagctgcta
     3241 tgtgaggttg gggcagaggg gtagggcgtt gaggcagtac cgcttgtgcg agaggattct
     3301 aggacaggag tatggcacta tcccttcgcc cgagatccag gacctctaca gaagcatcct
     3361 cggtgacgag ggcggcgcga gatctcgcgg cggcaatggt gggtccgggc tgggcgcgcc
     3421 atgagcaatc cctcctagct ccaggactgg cccgtaagcg cgctcttcgc ctcatctccc
     3481 gcgggcgctg gtggtttaag ggcgcggggt ttcgcgcatg aatgtgctat agccttcgag
     3541 gagaggatag acagtgagcg aaaagcgaac ccgacggagc agtgagcgaa gccccctcca
     3601 gagcgagcgc ggcacgacct ccatcagcta cccggtggtc gcccagatcg ccggcatcgc
     3661 ggcccaggag gtcgagaagg tgcagatggg cggcggcgca accgctgccg tcggcggctt
     3721 cctccagagc gtcacgggca gctcgtccgg gaactatgcc cgcggagtca ccgtcgaggt
     3781 cggtgaggaa gaagcggccg tggacctcac gatggccgtc gagtacggct actcggtccc
     3841 gcagctcacc gaagcggtgc gccgcaacgt catcaacagg gtcgagagcc tgaccggcct
     3901 gagggtcacg gaagtcaaca taacggtcaa cgacgtccag atcccagatg agcgcccgat
     3961 gctcgacgag caggagcagg tcgagcggca ggccaggcag caggagcagc aagcttagaa
     4021 cggaaccgca tttgcccggt caccctggag tgcgctcagg ggtagt
//
LOCUS       DASTUV010000032         6935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1461886, whole genome shotgun sequence.
ACCESSION   DASTUV010000032 DASTUV010000000
VERSION     DASTUV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461886"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFT03_00605"
     CDS             <1..333
                     /locus_tag="VFT03_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU4846670.1"
                     /translation="AVGGPLFEPALNSLAKKGRMVVITTTPDKQHVSFNLLDFYRKGL
                     RLFGLMTSFLDAEGSAAVLRDLSPGFDEGALRAPAIADRYPLEQAGMAYARVESGEAA
                     GRVLLLMS"
     gene            448..846
                     /locus_tag="VFT03_00610"
     CDS             448..846
                     /locus_tag="VFT03_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4846671.1"
                     /translation="MEIRNFRDVAEQVMTKADQEGLIVRNIINKDSAGARKIILLSVE
                     LPPGTVHLLHRHPNAEQVMYVLEGSCLALSEGEPVRLNEGDAVFIAQGEWHGVRNNTD
                     QSAVTLIIYAGAGTLEEAGYEEHPRQFDGA"
     gene            1374..2288
                     /locus_tag="VFT03_00615"
     CDS             1374..2288
                     /locus_tag="VFT03_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU4846672.1"
                     /translation="MATSELPFVLRLGDPACGDATQVGGKAARLSHLAADYRVPPGFC
                     LTAAAYDPTQSPGRPLSAVVAAEVAAAYEALSYDLGVPNVPVAVRSSALDEDGEIASF
                     AGQHETELNVVGVDAVLGAVARCWASARDERALAYRKQQGLGVARVRLAVLIQQLVLA
                     DASAVVFSANPVSGDRDEVVVTANWGLGESVVSGTVSPDTWIVLKAGLAVKEDQIGAK
                     ERMTVAVDGGTQEVSVPGLLRKRASLSRAQVAELAELAVQLEVKMGWPVDIETAFANE
                     LLYLLQCRPITTLTESDASATVPIQRRF"
     gene            2301..3983
                     /locus_tag="VFT03_00620"
     CDS             2301..3983
                     /locus_tag="VFT03_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEU4846673.1"
                     /translation="MQQGPISAPPDFPVVWENPEDEQLFWTRDAMHSPNQLSELEAEL
                     AIGIAHSYGFTSASEAYGLPVRLQPRLINTYYYMAAAPTMAPPEEMAAMEERAREKVG
                     AAMARLGEMWSTEFLPEIQRYLKDWDGFDLRGASMPQLLAHLDETVARHKRLWEIHFI
                     LAIPMLMSVSTFEEFYRELFGSEGAFDALRLLQGLDNKTVETGRELWRLSRKAREMPE
                     VRKVLEKSAAADVVGALERSAEGREFLVQLDEYLEEYGQRGDMWGVGYPTWAENPTTV
                     IKMLKDYLTQEGGGPEEEQASLAAERERLLAETRERLATYPQAVRDEFEFLLKAAEEG
                     VVLSEDHGFWIDFRSTARVRRVIMEFGRRFAEAGVIERPEDTFHLNLAEIRETAKRFP
                     DLVRKDVVARRKDELERFGKVQVPPALGTPPPGPPPDNPFNRALGKFFGGPPQPPEES
                     GVLRGNPGSPGVVRGRARVLRALSEGDGLQEGDILVAETTAPPWTPLFATAAAVVTDT
                     GGILSHCAVVAREYRIPAVVGTGAATATIRSGQIIEVDGDAGVVKILSEDGS"
     gene            4001..5377
                     /locus_tag="VFT03_00625"
     CDS             4001..5377
                     /locus_tag="VFT03_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4846674.1"
                     /translation="MKEGPVGRPLSEGMVLAPVALGTMLAPLNSTMIAVAIPSLLGDF
                     NRPLAWGAWIVTSYLVAMAAVQPLGGSLGDRYGQRRLFLIGLTLFLVATVAAALSWRI
                     EVLLVARTVQAIAGAAAIPNGTALVRSLVAPERRGRAFGNVGAAIAVAAGLGPPIGGI
                     LTAALGWRWIFAANLLLLAPALILGWRLPKDASSSQPRRFDLLGAALLTLGLVSLVLS
                     MTIWRLPNAPLLLAPVFGCIAAGTLLALVRHSGRSPNPVLNLALLRARGFTPAVATVL
                     LSNLTMYTLLLSLPLFLTAWSLWNSARVGLLLAGLSLPMIVFSPLGGRLSDNLGRRAP
                     AVLGTSLITAGTLPFLLVYPSWTWLLYLVPLVCIGAGVGLSMAPVQTTAIETAPYSQT
                     GQASGLFSTMRYLGSILGSSVMAAVLSGATPPVTHFRILYVMLFFSACGATIAALRLP
                     RWLNKPAR"
     gene            complement(6230..>6935)
                     /locus_tag="VFT03_00630"
     CDS             complement(6230..>6935)
                     /locus_tag="VFT03_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU4846675.1"
                     /translation="EDVHPVTYAIQPPPITTLPVAGTDESFPVSRVYCVGRNYAEHAV
                     EMGGDPDREPPFFFAKPANSVVANGGTLPFPSATEDLHFEIELVVALAEGGADLDASD
                     TLSHVFGYAVGLDMTRRDLQAEAKKAGRPWDMAKGFDRSAPMSAIRPVSAIGHPERGS
                     IWLRVNDELRQQGDLSQQIWKVPETIAFLSTLVELRPGDLIMTGTPKGVGPVSRGDRL
                     VGHIDGVGDLEVDYQA"
BASE COUNT         1222 a         2146 c         2276 g         1291 t
ORIGIN      
        1 gctgtcggcg gccccctgtt cgaaccggcc ctgaacagcc tcgccaagaa gggacgcatg
       61 gtcgtcatca cgacgactcc cgacaagcaa cacgtctcct tcaacctgct tgatttttac
      121 cgcaaggggc tgcgcctctt cgggctgatg acgtcgtttt tggacgccga agggagcgcc
      181 gccgtcctga gggacctgag cccggggttc gatgaagggg ccctacgtgc gccggcgatc
      241 gccgaccgtt acccgctaga gcaggcgggg atggcctacg cacgggtaga gagcggtgag
      301 gcggccggta gggtactcct cctcatgagt tgagcggccg atcgctggaa cgttccaata
      361 ctccttccgg ccgatcgaag tggttcctgg attgaggtat gggagttcgt tcatcggtgc
      421 gccagcgatt gagaagaaag gagggccgtg gagataagaa actttaggga cgttgccgag
      481 caggtgatga ccaaggccga ccaggaaggc ttgatcgtca ggaacatcat caacaaggac
      541 agtgccgggg cccgcaagat catcttgctc tccgtcgagc ttccgcctgg cacggtccac
      601 ctgctgcacc ggcaccccaa cgccgagcag gtcatgtacg tgctcgaggg ttcgtgcctg
      661 gctctctcgg agggagagcc ggtacgcctg aacgagggcg acgccgtgtt catcgcccag
      721 ggcgagtggc acggcgtcag aaacaacacc gaccagtcgg ccgtaacgct cataatctat
      781 gcgggagccg gaaccctaga ggaggctggc tacgaggagc accctcggca gttcgatgga
      841 gcgtaacgaa ggagatgcag ggaaaggggc atgtgcattg tagagaggaa ccagcaacac
      901 gcacttcacg gactcctgac agcactctga caccaccggg aacgcaatgc ggtgcaacac
      961 ctggcaaggc agagtaaaga aaccggccta gaaatgcggg atttgcaagg cggagcaata
     1021 ccctgcaata cctgactgat cactcgtagc tggagcaggg ataagcggtt ctcaagtccg
     1081 ctcgtcggct ctttatcctt cagtggcagg acgagaactt tttcttggca tcttcattca
     1141 ctggcacccc ttgaacgggc agacgttcga tcgtgctagc gaacgtctca cctatggctg
     1201 tcgtcgcgag aaaagcagaa aaatactccg tttgggggat gcgaagatcg attgcggagc
     1261 gtaaagtgcg tccgaattta acatgagttt aacgttgtgg tgcaacagga gcggcggccg
     1321 gatatggcga tctaaccggc ggcccggcgg aagagagggg acagacgatg acaatggcga
     1381 cgagcgagct gccatttgta ctgcgactcg gtgaccctgc gtgcggcgac gctactcagg
     1441 tcggcggcaa ggcggcccgt ctcagtcatc tagccgccga ctatcgtgtg ccgccaggtt
     1501 tctgcctgac cgctgccgcc tacgacccga cgcagtcgcc tggcaggccg ttgtcggcgg
     1561 tcgtggctgc ggaggtcgcc gccgcctacg aggcgctatc ctatgatctt ggcgtgccca
     1621 atgtcccggt cgcggtacgg tcctcggcgc tcgacgagga cggggagatt gcgtcgttcg
     1681 caggtcagca cgagacggag ctgaacgtgg ttggggtcga tgctgtcctc ggcgccgtcg
     1741 cccgttgctg ggcctcagca cgcgacgagc gggcgctcgc gtaccgcaaa cagcagggat
     1801 taggggtcgc gagggtcagg ctcgcggtgc tgatccagca gctcgtcctc gccgacgcct
     1861 cggcggtggt cttcagcgcc aacccggtat caggggaccg ggacgaggtg gtcgtgacgg
     1921 cgaactgggg gctcggggag agcgtggtgt cggggacggt ctcgcccgat acttggatag
     1981 tcctcaaagc aggtctggca gttaaagagg atcagatagg ggcgaaggag cggatgacag
     2041 tagctgtgga cgggggaacg caggaggtta gcgtgcccgg tctattgcgc aagcgggctt
     2101 ccttgagcag agcccaggtc gcggagctgg cagagctcgc ggtgcagctt gaagtgaaga
     2161 tgggttggcc tgtggacatc gagacggcct tcgcaaacga gcttctctac ctgctccagt
     2221 gccgtccgat caccacgctt accgagtcag acgcctccgc cactgttccg attcaaagac
     2281 gcttttaagg aggaccagtt atgcagcaag gcccgatctc ggcgccgcca gacttcccgg
     2341 tcgtttggga gaaccctgag gatgagcaac tcttctggac gcgcgacgcg atgcactccc
     2401 cgaaccagct aagcgaactg gaggccgaac tggccatcgg gatcgcgcat tcgtacgggt
     2461 ttaccagcgc ctccgaggcc tacggtctac ccgtacgcct ccagccgcgt ctcatcaaca
     2521 cctattacta catggctgcg gcaccgacga tggcgccgcc tgaggagatg gcggccatgg
     2581 aggaaagggc acgcgagaag gtcggggccg cgatggcacg gcttggcgag atgtggtcca
     2641 ctgagttctt gcccgagatc cagcggtacc tgaaagactg ggacggcttc gacctacgcg
     2701 gcgcttcgat gccgcagctc cttgcgcacc tggatgagac cgtggccagg cacaagaggc
     2761 tgtgggagat ccacttcatc ctggctatcc cgatgctgat gtcggtaagc acattcgagg
     2821 agttctaccg ggaactcttc ggatccgagg gagccttcga cgccctccgc ttgttgcagg
     2881 ggctggacaa caagacggtc gagaccggcc gcgagctctg gcggctcagc cggaaggctc
     2941 gagagatgcc cgaggtgcgc aaggtcctgg agaagagcgc cgccgcagac gtcgtggggg
     3001 cgctcgagcg ctcggcggag ggccgagagt tcctcgtgca actcgacgaa tacctcgagg
     3061 agtacggcca gcggggcgac atgtgggggg tgggctaccc gacctgggcc gagaatccca
     3121 cgaccgtcat caagatgctg aaggactacc tgacccagga gggcggcggt cccgaggagg
     3181 agcaagcgtc tttggcggcc gagcgggagc gcctcctggc cgaaacgcgt gagcgcctcg
     3241 ctacctaccc gcaagcggtt cgcgacgagt tcgagttcct cctcaaggcg gccgaggaag
     3301 gtgtggtctt gagcgaggat cacgggttct ggatcgactt caggtcgacg gccagggtgc
     3361 gcagggtcat catggagttc ggtcgtcgct tcgccgaggc cggcgtcatc gagaggcctg
     3421 aagacacctt ccacctgaat ttagccgaga tacgcgagac cgcgaagcgg ttcccggacc
     3481 tcgtcaggaa ggacgtcgtc gcccgacgca aggatgaact ggagcggttt gggaaggtac
     3541 aggtgccccc ggccctgggt acaccgccgc cgggcccgcc acccgataat cccttcaacc
     3601 gcgcgctagg gaaatttttc ggagggccgc cccagccgcc cgaggagtcc ggcgtcttgc
     3661 gaggcaaccc cggctcgcct ggcgtagtgc gtggccgcgc cagggtgctc cgtgcgctct
     3721 ccgagggtga tggacttcaa gaaggcgata tccttgtagc ggagaccacc gccccgcctt
     3781 ggacgccgct gtttgcaacc gcagccgcag tggtcaccga caccggcggc atcctcagcc
     3841 actgcgccgt ggtggctcgc gagtatcgca tcccggccgt ggtggggacc ggcgcagcga
     3901 cggcgacgat aaggagcgga cagatcattg aggtggacgg ggacgcgggt gtcgtaaaga
     3961 tactttccga agacggaagc tgagtaacgt aaacacgggc gtgaaagagg gccccgtagg
     4021 gaggccgcta tccgagggca tggtgctcgc ccccgttgcg cttggcacca tgctcgctcc
     4081 cctcaactcg accatgatcg ccgtggcgat cccctcgttg ctgggggact tcaaccgccc
     4141 cctcgcctgg ggtgcctgga tcgtgacttc atacttggtc gccatggccg ccgtccagcc
     4201 gctcggcggc tccttggggg accgatacgg ccagcggagg ctcttcctga tcgggctcac
     4261 tctctttctc gtggctacgg ttgctgctgc gctctcatgg cgcattgagg tcctgctggt
     4321 cgcccggacc gtgcaggcca tcgccggcgc cgccgccatc ccgaacggca cggcgctggt
     4381 ccgctcgctc gtcgcccccg agcgccgggg ccgcgcgttc gggaacgtgg gggctgccat
     4441 cgcggtagcg gccgggctcg gtcccccgat cggaggcatc ttgaccgccg ccctcgggtg
     4501 gcgttggatc ttcgccgcta acctcctgtt gttggcaccc gccttgatac ttggatggag
     4561 gctgccgaag gatgcctcgt cttcacaacc gcgccggttc gacctgctcg gtgcggccct
     4621 cctaaccctc ggcctggtct ccctggtact ctcgatgacg atctggcggc tgccaaacgc
     4681 cccgttatta ttggcgccgg ttttcggctg cattgcggcc ggtactctcc tggcgctagt
     4741 ccgacactcg gggcgttccc cgaatcccgt gttgaacctt gcgctgctgc gggcgcgcgg
     4801 gttcacgccc gcggtcgcca ccgtgctgct cagcaacctg accatgtaca cactgctcct
     4861 gtccctgccg ttattcctga cggcgtggag cctgtggaac agcgctcgcg tcggcttgct
     4921 cctggcgggg ctctcgctgc cgatgatcgt cttctccccg ctggggggcc gcctctcaga
     4981 caacctcggg aggcgcgcgc cggccgttct cgggacctcc ttgatcacgg cgggcaccct
     5041 cccttttcta ctggtctacc cctcctggac atggctactc tatctagtcc cgctcgtctg
     5101 catcggcgcc ggggtggggc tctctatggc tccggtccag acgacggcga tagagaccgc
     5161 cccctatagc cagaccggtc aggcgtcagg tctcttttcc actatgcgct acctcgggag
     5221 catcttggga tcatccgtca tggcagcggt cctgagcggg gccaccccgc cggtcactca
     5281 cttccgcatc ctctacgtta tgctcttttt ctcggcctgc ggcgcgacga tcgcagccct
     5341 gcgcctgccg agatggctca acaagcctgc tcgctgatca gagggcttca gcttgcgttg
     5401 gaaggtattg ctcgctctag ttgaaggcca ggaaaaaggg agcgccgtgt gcgcccttgc
     5461 gtcggcgata tacaagtggt tgcagggccc agcaggtggc gttggcaatt cctctggcat
     5521 tgagcgtttg accgatgtgc cggagtttcg gcgagtacgg cgccgtggaa agagcttcgc
     5581 ttgagtgccg aaaaggtaag tgttcggcga agttgggcgt ccgctttacg gcgttctgct
     5641 aacttcggta tgtacggtat cctcagaagc cccagcggcg tgaccgcgca ccccgcgtcc
     5701 cgcatgccta aacttcgctc accgccacga ctacgaagag gagggggaga tggaccggga
     5761 ggtctatact ggctggtagc gggcatggtc ttctcgatcg tggtggcttt gttcgaggcg
     5821 tgggtgttgc tcgtcgaaat caaccggtag gcgaagaccg agatccttct caccacgtcg
     5881 tgcacacgag cgcgtggaag aagttgtgtt ctccgaagtg ggcaatgcag agaactgaaa
     5941 ctggcctaga aatgcgggaa ttgcatacgc ctgccaacga ccaccaacca ctgaccgcac
     6001 actcgtagct ggagcaggga taagcggtgc gagtccgcgc atgatccaaa cccccgcaac
     6061 tcgcctccgc aatatgcggg tcctagctcg ggcgtccgag gacagcctgg ttgcgaaagg
     6121 ttctgttaac gccgcacgga ttgcctggcg gtctctgaac gcacgcccgg tgtgctgcat
     6181 tctgggttag cctgagccct ggctacgtcc tccaacgtcg ctgagcacgt cacgcttggt
     6241 agtcgacctc caggtcgccc actccgtcga tgtgtcccac cagacgatcg cctcggctca
     6301 ccggtccgac tcccttaggt gtaccggtca tgatcaggtc accgggtcgc agctccacca
     6361 gggtgctcag gaacgcgatg gtctcgggaa ctttccagat ctgctggctg aggtccccct
     6421 gctgccggag ctcatcgttg acgcgcagcc agatcgagcc ccgctccggg tggccgatcg
     6481 ccgataccgg acgaatcgcg ctcatcgggg cggaccggtc gaagcctttg gccatgtccc
     6541 agggccgtcc ggccttcttc gcctcagcct gcaggtcgcg gcgggtcatg tcgagcccta
     6601 cggcgtaccc gaacacgtgg gatagggtgt ccgatgcgtc caggtccgca ccgccctcag
     6661 ccagcgccac caccagctcg atctcgaagt gcaggtcctc cgttgccgac gggaacggca
     6721 gggtcccgcc gttcgctacc acggagttgg ccggcttcgc gaagaagaac ggtggttccc
     6781 ggtccgggtc gccgcccatc tccacggcgt gctcggcgta gttgcgtccc acgcagtaaa
     6841 cccgggagac cgggaagctc tcgtccgtcc cggccacggg cagggtggtt ataggcggcg
     6901 gctgtatggc gtaggtcaca ggatgcacgt cctct
//
LOCUS       DASTUV010000033         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1784435, whole genome shotgun sequence.
ACCESSION   DASTUV010000033 DASTUV010000000
VERSION     DASTUV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784435"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="prfB"
                     /locus_tag="VFT03_00635"
     CDS             <1..690
                     /gene="prfB"
                     /locus_tag="VFT03_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU4846676.1"
                     /translation="DSQDWAEMLSRMYRRWAERRGFEVEVIEYTEGEEAGIKSATFTV
                     SGEYAFGLLSAERGVHRLVRISPFDASSRRHTSFASVAVAPVIDDRVEVEIDEKDLKI
                     DTYRASGAGGQHVNKTDSAVRITHLPTGIVVQCQNERSQHQNREVAMRVLRARLFELE
                     RERREQEIAAQSGEKADIEWGSQIRSYVLHPYKLVKDHRTGEETANVDRVLDGDLDDF
                     IYAYLKNRAVA"
     gene            695..1396
                     /locus_tag="VFT03_00640"
     CDS             695..1396
                     /locus_tag="VFT03_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEU4846677.1"
                     /translation="MSGKTLFALLILVFIVLAPFTVLPALLESATARLLQDQLGLEEA
                     PEVEIESTPAPMMYAGSFSRALISAKGIEIAGVKTSNIIMDLDPFNLNIIESATSGEV
                     STEQPLSGKLSVELSEDNVSQLAQVGSGVPVQDVQLEDDLVVLEMSLGFGAPVTVRGR
                     LFLQNGLLIFQPEQVEGAPAFVSSEQLLALTRFSYPLTNLPFGARVTGVKVRKGSLIL
                     SGYIQEIPLGGIPAS"
     gene            1506..2192
                     /gene="ftsE"
                     /locus_tag="VFT03_00645"
     CDS             1506..2192
                     /gene="ftsE"
                     /locus_tag="VFT03_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU4846678.1"
                     /translation="MIRFDNVTKLYGKDTVALERVSLEIEPGEFVFLVGASGSGKSTM
                     VRLILKEIEPSAGAIYFKGVRLSSVPRRKIPRHRRNIGTVFQDFKLLPNKTAAENVAY
                     AMEVTGQRRRAIRTKVPQILDLVGLSGKMDKYPDMLSGGEQQRVSIARAFVSQPPLLV
                     ADEPTGNLDPNTSVGIMQLLHRINRIGTTVLVATHDKEMVDVMRKRVVALEEGRVVRD
                     KVRGAYADEV"
     gene            2182..>2605
                     /locus_tag="VFT03_00650"
     CDS             2182..>2605
                     /locus_tag="VFT03_00650"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU4846679.1"
                     /translation="MRFNFGFFMREAFKNLRLNLLMSITAITTTFICIVVLGVAWLVS
                     AHVGGVTSSVREDVSVEAYFPPDATQEEIDGIISDVKSWPEVSGVNYVSEEEALAAFK
                     ETFADRPELSENLEPGVLPASIQIQLEDPSEAEAVAQRL"
BASE COUNT          510 a          792 c          846 g          457 t
ORIGIN      
        1 gactcgcagg actgggccga gatgctctcc cgcatgtacc ggcgctgggc cgagcgcagg
       61 ggcttcgagg tcgaggtcat cgagtatacg gagggcgagg aggccggcat aaagagcgcc
      121 accttcaccg tctcgggcga gtacgccttc ggactacttt ctgccgagcg cggtgtccac
      181 aggctggtga ggatcagccc gtttgacgca agttcccgaa gacacaccag cttcgcgtcg
      241 gtcgccgtcg cgcccgtcat tgacgacagg gtcgaggtcg agatcgacga gaaggacctc
      301 aagatagaca cataccgggc ctctggggca ggcgggcagc acgtgaacaa gaccgattcg
      361 gctgtacgca taacgcacct gccgacgggc atcgtagtgc agtgccagaa cgagcggagc
      421 cagcaccaga accgcgaggt cgccatgcgc gttcttcggg ccaggctctt cgagctggag
      481 cgcgagaggc gcgagcagga gatcgccgcg cagagcggtg agaaagctga tatcgagtgg
      541 gggtcccaga tccggtccta cgtacttcac ccctacaagc tggtcaagga ccaccggacc
      601 ggcgaggaga ccgccaacgt ggaccgcgtc ctcgacggcg acctggacga cttcatctac
      661 gcctacctga agaacagggc cgtcgcctag cgccgtgtcc gggaagacgc tgtttgctct
      721 cctgatactg gtttttatcg tgctcgctcc tttcaccgtc ctgccggcgc tgctggaatc
      781 ggcgaccgcg cgactcctgc aggaccagct cgggctcgag gaggcgcccg aggtggagat
      841 cgagagcact cccgcaccga tgatgtacgc gggtagcttc tcgagggccc tcatttcggc
      901 gaaggggatc gagatcgccg gagtgaagac cagtaacata atcatggatc tcgacccgtt
      961 caacctgaac attatagaga gcgcgacgag cggcgaggtg agcaccgagc agccgctctc
     1021 gggtaagctg agcgtggaac tctccgagga caacgtctcg caactcgcgc aggtcgggtc
     1081 cggggtgccc gtgcaggacg tccagctcga ggacgacctg gtcgtcctgg agatgtccct
     1141 cggcttcggg gcgccggtca ccgtgcgggg gcgcctgttc ctgcaaaacg ggctgctcat
     1201 cttccagcct gagcaggtcg aaggtgcgcc cgccttcgtc tcttccgagc aactgctcgc
     1261 gttgacgcgc ttctcgtacc ctctaacaaa cttgccgttc ggcgccagag tcacgggcgt
     1321 aaaggtccgg aagggcagcc ttatcctctc cgggtatata caggaaatcc cgttgggcgg
     1381 gatccccgcc agttgataaa gcccaccttt gttgttatct tcccccgtgg ccggttgaga
     1441 cattggaggt tcaggccgcc gtctgcggcg catatcacgc ccggcgcgtc acatctggag
     1501 aggtaatgat ccgattcgac aacgtcacca agttatatgg taaagacacg gtcgcgctcg
     1561 agcgtgtgag cctggagata gagccaggcg agttcgtgtt cctggtcggg gcgagcggct
     1621 ccggcaagtc cacgatggtg cggctgatcc tgaaggaaat agagccttcg gctggcgcta
     1681 tctatttcaa gggagtcagg ctctcgtctg tgccgcggcg caagatcccg cgccaccggc
     1741 gcaacatcgg gaccgtcttc caggacttca agctgctccc gaacaagacg gctgccgaga
     1801 acgtcgccta cgcgatggag gtgacaggcc agaggcgccg cgccatccgc acgaaggtgc
     1861 cccagatact agatctcgtg ggcctctcgg ggaagatgga caaatacccg gacatgttgt
     1921 cgggcggcga gcaacagcgc gtctccatcg cgagggcctt cgtctcgcag ccgccgctgc
     1981 tcgtcgccga cgagccaacc ggcaacctgg acccgaacac gagcgtcggc ataatgcagc
     2041 ttctgcaccg gatcaaccgc ataggcacca ccgtgctcgt cgccacccac gacaaggaga
     2101 tggtggacgt gatgcgcaag agggtcgtag ccctcgaaga ggggcgcgtg gtcagggaca
     2161 aggtgagggg ggcctatgcc gatgaggttt aatttcgggt tctttatgcg ggaggcattc
     2221 aagaacctcc gcttgaacct cctgatgagc atcactgcta taacgacgac gttcatctgc
     2281 atcgttgtcc ttggggtcgc atggctggta agcgcgcacg tcggaggggt gacctcctcc
     2341 gtgcgcgagg acgtgagcgt cgaggcgtat ttcccgccgg acgccacgca ggaggagata
     2401 gacggcataa tctccgacgt caagagctgg cccgaggtct ccggcgtcaa ctacgtctcc
     2461 gaggaggagg cgctcgcagc tttcaaggag accttcgcgg acaggccaga gctctccgag
     2521 aacctggaac ccggcgtcct gccagcgtcg atccagattc agctggagga ccccagcgag
     2581 gccgaggccg tggctcagag gctcg
//
LOCUS       DASTUV010000034         8277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1677090, whole genome shotgun sequence.
ACCESSION   DASTUV010000034 DASTUV010000000
VERSION     DASTUV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677090"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFT03_00655"
     CDS             <1..607
                     /locus_tag="VFT03_00655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEU4846680.1"
                     /translation="IAGPVWLRFSIEGETGHAGATPMSLRRDALAAAAEIISLIEAEA
                     SGTGTSVGTVGQLSLEPGGINIIPGLVEFSLDLRDIDEEVRDGVETRILEGAKEICRR
                     RGVELEIETLQRLAPAPCSELVRDAVAGACERVGIRAHALSSGAGHDGMQLTELCPMG
                     MIFVRSRDGVSHSPDEWSSREDCAKGSHVLYWTVLDLAGGD"
     gene            591..1820
                     /locus_tag="VFT03_00660"
     CDS             591..1820
                     /locus_tag="VFT03_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU4846681.1"
                     /translation="MQAGISPGDVAIATASFDDVVNDVREEVVAWRRHLHRNPEVSYQ
                     EDETSRFVYETLESFGGLELERPTKTSVVARLAGAGPGRTLAIRADMDALPIQEENDF
                     EFASQNPGAMHACGHDGHTAMLLGTAKILSEMKDRIRGEVRFVFQHAEELSPGGGEEL
                     VEAGVMDGVDAVIGAHLWAKLPVGKIGVTYGPMMAAPDIFEVTIKGRGGHAAMPHQLV
                     DSIAVGAQVVTNLQHVVSRATDPIDDVVVSVSKFSGGTTHNVIPGTVEMVGTIRTLDP
                     DVREMVPELVERVIKGVTQAHGAGYEFRYKPGYRPVINDEEVTREIEETVKDVFGEDA
                     LEIMPPNMGGEDFSAYQQRAPGTFFYVGAGNEEKGIVHPHHHPLFTIDEDSLEYGVKM
                     FINATFRLLDGAERSQG"
     gene            1817..2017
                     /locus_tag="VFT03_00665"
     CDS             1817..2017
                     /locus_tag="VFT03_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEU4846682.1"
                     /translation="MRPINLLAVLPFIGILVGTPFVNRVEPYVLGMPFILFWIVMWVV
                     LSSIIMAIVYAVDPANKEGDAE"
     gene            2014..3480
                     /locus_tag="VFT03_00670"
     CDS             2014..3480
                     /locus_tag="VFT03_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU4846683.1"
                     /translation="MNIALVIIFIFLAFAVFLGIRARRGKDMHLEEWTVGGRGFGAIF
                     IFLLLAGEIYTTFTFLGGSGWAYGEGGPAFYIIAYGCLAYIMSYWLLPAVWRYAKDNR
                     LHSQADFFASKYESSALGVLVSLVAVIAMVPYLALQFQGLGLIVSEASYGAISATVAA
                     IIGTVAVTVYVIISGIHGSAWTAVVKDIMVLVVVVFLGIYLPFALYGGVGEMFREIQA
                     QKPGFLILPEEGYSPSWFVSTVLLTALGFYMWPHTFGSTFTAENARVIRRNATFLPLY
                     QLILLFVFFVGFAAVLKVPGLTGEKVDLALLAISKDTFSAPVVGLIGAAGLLTALVPG
                     SMLLMVSATVLAKNVYSVFVPSTSEHRVGTIAKVLVPIVAAVSLWFSLQSGEGIVLLL
                     LLGYSLVTQLFPALLFSLMPNNFVTKWGAAAGIVAGVLTVTYVTVKETTIGELFPFLP
                     GAVADTNIGIVALVVNFVVLIIVSLATGGLAVRREKSA"
     gene            3546..3713
                     /locus_tag="VFT03_00675"
     CDS             3546..3713
                     /locus_tag="VFT03_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846684.1"
                     /translation="MIRRFTPETNRLVGLAGSYGRESMATEIGTGVVEEIVPGGGPLG
                     WRAAEPGTGGA"
     gene            3710..5638
                     /gene="iolC"
                     /locus_tag="VFT03_00680"
     CDS             3710..5638
                     /gene="iolC"
                     /locus_tag="VFT03_00680"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEU4846685.1"
                     /translation="MNEGAQFELDLVCIGRTSVDLYAEQEGARLQDVQSFRKYVGGSA
                     TNIAVGTARLGVESAMLTRVGNEQMGRFVRRTLAENGVDVSYVLFDPEHLTPYVLLAV
                     RAIEDFPRIFAYGDAADLALREEDIDPDFIASSKALLVTGTPFSRPGPRAASVKAIRA
                     ANEAGTGVVFDLDYRPVFWGVATHEQGGEMFVASEKVTEVYRSALPGCDLVVGTEEEI
                     RIAGGSTDTIEALRSIREISGATIVLKVGAMGAIVFPEDIPDDVEGGVSVAGFPVEVF
                     NSVGAGDAFMSGFLSGWLREEPLEECLRLGNACGAIVVSRHGCSPAMPTREELEYFLT
                     MPERPRSLRDDEWLSHLHRATTRNDPEELRVLAIDHRWQLEEVADELGVDREDLRELK
                     VLLGRAFRRVAEEEAAAGVLLDDVYGDKALEELTGSGLWISRAVEVAGSRPVEFVGGP
                     NVAATLRSWPQEHVVKCNLYWHPEDEPEITDLQQQRVFQLFDACVNNDRQLLLEVQAP
                     QGMPYDEGSMAQLLARFYEIGVRPDRWKLPPNPDPGVWKRIGGVVREHDPYCAGLLVL
                     GQAAGEEELAESFAAAASEPLCNGFAIGRSIYGDPARRWLAGEIGDEELISSVAERYR
                     RMISLWRSRNEPRRGATV"
     gene            5673..6728
                     /locus_tag="VFT03_00685"
     CDS             5673..6728
                     /locus_tag="VFT03_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4846686.1"
                     /translation="MNTGKRAGIEQVSVGVVGAGGMGGVHARNLRSRIAGASLAAIAD
                     VDADRAARVAGKNVPVFGDAVEMIRDDGVEAVVIASPSATHAGLTLECLRLGKPVLCE
                     KPLSTTAEDALEVVEAEVELGTKLVQLGFMRRFDRQHLTVKEVVDSGSIGAPLLFKGW
                     HRNPHIMSSTATSESVVIDSMIHDLDSVRWMFGSEIDEVYTRGISTRPDLEGACDLQL
                     TQISVGGGLLATVEVYVTAEYGYEVGLEVVCESGTAHVAPFDEAVVRRDATLYQRIHT
                     DPFARFQPAFVAEMERWTGAIQNGEQPDGPDAWDGYAALVAAVSCIESLRSGSPRSVT
                     VPERPALYSKEDAGVVW"
     gene            6725..7615
                     /locus_tag="VFT03_00690"
     CDS             6725..7615
                     /locus_tag="VFT03_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU4846687.1"
                     /translation="MREEARVAGAPVSWGVIEVPDWGYQMPADRVLREAAALGLAAIE
                     AGPEGFLPQDPAEVSDLLAGYGLSLVGGFVPAVLHDAGSREAQLALVERRAEFFAAAG
                     AEVVVLAAATGSESYEEVAELDDGAWRELFESLSTVEEICARHGLTLALHHHYGTVIE
                     RDDQLRRFLEGCETGLCLDTGHLVLGGSDPVEIAELAGDRVSHVHLKDVDRDLGRRVA
                     TRELSFKEAAGRGAFRPLGDGDVDVAEVVARLERSGYSAWYVLEQDTVVETEPDEGEG
                     PTGDVRKSLAYLLRRLERTA"
     gene            7636..>8277
                     /locus_tag="VFT03_00695"
     CDS             7636..>8277
                     /locus_tag="VFT03_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU4846688.1"
                     /translation="MDTIRLTTAQALVRFLANQYVERDGVEQRLFAGAFGIFGHGNVA
                     GVGQALHQYGDELTYYQPRNEQAMVHTAAAYARQKNRLQTLVCTSSIGPGATNMVTGA
                     ALATINRLPVLLLPGDVFASRAPDPVLQQLEAPYDGTLSVNDCFRPVSKYWDRIDRPE
                     QLIPAALAAVRVLTNQAETGAVTLALPQDVQAEAYDFPEEFFETRVWHVPRQLP"
BASE COUNT         1401 a         2610 c         2871 g         1395 t
ORIGIN      
        1 catagcaggc ccggtatggc tccggttttc gatcgaggga gagaccgggc acgcgggtgc
       61 gacgccgatg agcctccggc gcgacgcgct cgcggcggcg gcggagatca taagcctcat
      121 cgaggccgag gcctcgggta caggcacgag cgtgggtacc gttggccagt tgagcctcga
      181 gccgggcggc atcaacatca tccctggcct ggtcgagttc tcgctcgacc tcagggacat
      241 agacgaggaa gtcagggatg gggtcgagac ccgcatcctg gaaggggcaa aggagatctg
      301 ccgcaggcgc ggtgtggaac tcgagatcga gacgctgcaa cggctcgcgc ccgcgccgtg
      361 ctcggagctc gtccgtgacg ccgtggctgg ggcttgcgag agggttggca tccgagctca
      421 cgcgctctcc agcggcgcgg ggcacgacgg gatgcagctc acggagctgt gcccgatggg
      481 catgatcttc gtccgctcca gagacggcgt cagccacagc cccgacgagt ggagctcgcg
      541 cgaagattgc gccaaaggta gccacgttct atattggacg gtgctggatc ttgcaggcgg
      601 ggattagtcc gggggatgta gccatcgcaa ccgcatcgtt cgacgacgtc gtaaacgacg
      661 tcagggaaga ggtagtcgcc tggcggcggc acctgcaccg caaccctgag gtctcctacc
      721 aagaggatga gacatcccgt ttcgtctacg agacgctgga atcgttcggc gggctggagc
      781 tggaaaggcc gacgaagacc agcgtcgtcg ccaggctcgc cggtgcgggc ccggggcgca
      841 cgctggcgat ccgggccgac atggacgcgc tgcccataca agaggagaac gacttcgagt
      901 tcgcctcgca gaaccctggc gcgatgcacg cctgcgggca cgacggccac acggccatgc
      961 tgcttgggac cgcgaagatc ctctcggaga tgaaggaccg tatccggggc gaggtgcgct
     1021 tcgtgttcca gcacgccgag gagctctccc ccggcggcgg ggaggagctc gtcgaggccg
     1081 gggtgatgga cggggtggac gctgtcatag gcgcgcacct gtgggcgaag ctccctgttg
     1141 gcaagatcgg ggttacctac ggtcccatga tggccgcgcc ggacatattc gaggtcacga
     1201 tcaagggcag gggcggccac gcggcgatgc cgcaccagtt ggtcgacagc atcgccgtgg
     1261 gcgcgcaggt cgtgactaat ttgcagcacg tcgtatcgcg cgcgacagac cccatagacg
     1321 acgtggtcgt ctcggtgagc aagttctccg gaggcaccac gcacaacgtc atccccggca
     1381 cggtcgagat ggttggcacg atccgcaccc tcgatcccga tgtcagggag atggtgcccg
     1441 agctggtgga gcgcgtgatc aagggcgtca cgcaggccca cggagccggc tacgagttcc
     1501 gatacaagcc gggctaccgg cccgtgatca acgacgaaga ggtaacccgg gagatagagg
     1561 agaccgtgaa ggacgtcttc ggcgaggacg ctctggagat catgcctccc aacatgggcg
     1621 gcgaggactt ctcggcctac cagcagcggg cgccggggac cttcttctat gtcggggcgg
     1681 gcaacgagga gaaaggcatc gtccaccctc accaccaccc gctcttcact atagacgagg
     1741 actctttaga gtatggggta aagatgttca ttaatgccac gttcaggctg ctggacgggg
     1801 cggagaggag ccaggggtga ggccgatcaa tctgctggcg gtgctcccgt tcatcgggat
     1861 actcgtcggc acgccgttcg taaacagggt cgagccctac gtgctcggga tgccttttat
     1921 cctgttctgg atcgtgatgt gggtggtcct gagctcgatc ataatggcta tcgtctacgc
     1981 cgtagacccc gccaacaagg agggtgacgc cgaatgaaca tcgcgctcgt catcatcttc
     2041 atcttcctcg ccttcgcggt gttcctcggc atcagggcgc ggcggggcaa ggacatgcac
     2101 ctcgaggagt ggaccgtagg ggggcggggc ttcggcgcga tcttcatctt cctgctgctt
     2161 gcgggcgaga tctacacgac cttcaccttc ctcgggggat caggctgggc ctacggcgag
     2221 ggagggccgg ctttctacat aatcgcctac gggtgcctgg cctacatcat gtcttactgg
     2281 ctgctccccg ccgtctggcg ctacgccaag gacaaccgcc tgcactccca ggctgacttc
     2341 ttcgccagca agtacgagag ctccgcgctc ggcgtgctcg tctccctcgt agccgtaatc
     2401 gcgatggtcc cctacctcgc gctgcagttc cagggactcg ggctcatcgt ctccgaggcc
     2461 tcctacggcg ctatctccgc gaccgtcgcc gcgatcatcg ggacggtcgc tgtaaccgtc
     2521 tacgtgatca tcagcggcat ccacggctct gcctggacgg cggtggtcaa agacatcatg
     2581 gtgctcgtgg tcgtcgtctt cctcgggatc tacctcccct tcgcgctcta cggcggggtg
     2641 ggggagatgt tcagggagat acaggcccag aagccaggct tcctgatcct ccccgaggag
     2701 ggctacagcc cttcgtggtt cgtctcgacg gtgctgctca ccgctctggg tttctacatg
     2761 tggccgcaca cgttcggctc cacgttcacg gccgagaacg cgcgcgtgat ccggcgcaac
     2821 gccaccttcc tgccgctgta ccagctgatc ctcctcttcg tcttcttcgt cggcttcgcg
     2881 gccgttctga aggtccccgg cctcacgggc gagaaggtcg acctcgcgct cctggcgatc
     2941 tccaaggata cgttcagcgc gcccgtcgtc gggctcatag gcgccgcagg cctcctgacc
     3001 gccctggtcc ccggctcgat gctcctgatg gtgtccgcga cagtgctcgc caagaatgtc
     3061 tacagcgtat tcgtgccctc gaccagtgag catagggtcg gcaccatagc caaggtactc
     3121 gtccctatcg tcgcggccgt gtccctctgg ttctccttgc agagtggcga ggggatagtc
     3181 ctcctgctcc tcctcgggta cagcctcgtg acccagctct tccccgctct gctcttctcc
     3241 ctcatgccca acaacttcgt gaccaagtgg ggggccgcag ccggcatcgt cgccggcgtc
     3301 ctgaccgtca cctacgtgac cgtcaaggag acgaccatag gcgagctctt cccctttctg
     3361 ccaggcgcgg tcgccgatac gaacataggt atcgtagcgc tcgtcgtgaa cttcgtggtg
     3421 ctcattatcg tgagcctcgc cacaggcggc ctcgccgtcc gcagggagaa gagcgcctag
     3481 agaccggcac agaataacgg tcacgccata gaatacggct cgctcgagat gatgctgatg
     3541 gagctttgat ccggcgcttc acgccggaaa cgaaccgcct cgtcggtctg gccggcagtt
     3601 acggaaggga gagcatggcg acggagatcg gcacaggggt ggtcgaagag atcgtgcccg
     3661 gtggtggccc gttgggatgg cgggcggcgg agcctgggac gggaggggca tgaacgaagg
     3721 ggcacaattc gagctggacc tcgtatgcat cgggcgcacc tccgtggacc tctatgccga
     3781 gcaggaggga gctcgcctac aggacgtcca gtccttccgc aagtacgtgg gcggcagcgc
     3841 gacgaacata gccgtcggta cggcgcggct gggggtagag agcgcgatgc taacccgcgt
     3901 cgggaacgag caaatgggac gtttcgtgcg ccggacgctg gcggagaacg gcgtggacgt
     3961 gagctacgtc ctgttcgacc cggagcatct caccccctac gttcttctcg ccgtcagggc
     4021 gatcgaagac ttcccgagga tcttcgccta cggcgatgcc gccgacctcg ctctccggga
     4081 ggaagatatc gacccggact ttattgcctc ctcgaaggcg ctgctggtca cgggaacgcc
     4141 cttctcgcgc cccgggccgc gggcggcctc agtcaaggcg attcgggccg cgaacgaggc
     4201 ggggaccggg gtggtgttcg acctggacta ccggcccgta ttctggggtg tggccacaca
     4261 cgaacagggc ggcgagatgt tcgtcgcgag cgagaaggta acggaggtct accgctcggc
     4321 gctgcctggc tgcgacctcg ttgtcggcac ggaggaggag atccggatcg ccggcggctc
     4381 taccgacacc atcgaggcgc tacggagcat ccgcgaaatc tccggcgcga ccatcgtgct
     4441 caaggtcggc gcgatgggag ccatagtctt cccggaagac atcccagacg acgtcgaagg
     4501 cggcgtgagt gtcgccgggt tccccgtaga ggtgttcaac agcgtgggag caggagacgc
     4561 tttcatgagc ggcttcctct ccggctggtt gcgcgaggag cccctggagg agtgtctgcg
     4621 cctcggtaac gcctgcggcg ccatagtcgt ctcccggcac gggtgctccc cggccatgcc
     4681 tacccgagag gagctcgagt acttcctgac catgcccgag aggccacgct ccctccggga
     4741 cgacgagtgg ctcagccacc tccaccgcgc gactacccgc aacgaccccg aggagcttcg
     4801 cgtgctggcc atagaccacc gctggcagct cgaagaggta gccgacgagc tcggggtgga
     4861 ccgcgaagat ttgcgtgagc tcaaggttct gctcggaagg gcgttccggc gcgtggccga
     4921 ggaagaggcg gcggcgggcg tgctgctcga cgacgtctac ggtgataagg ctctcgagga
     4981 gctcacgggg agtgggctct ggatctcccg tgccgtagag gtggccgggt cccggccagt
     5041 ggagttcgtc gggggaccca acgtcgccgc cacgctgagg tcctggcccc aggagcacgt
     5101 ggtcaagtgc aacctgtact ggcacccgga ggacgaaccg gagatcacgg acctccagca
     5161 acagcgggta ttccagctct tcgacgcctg cgtcaacaac gaccggcagt tgctcctgga
     5221 ggtccaggct ccacagggca tgccctacga cgaaggcagc atggcgcagc tgctcgcccg
     5281 gttctacgag atcggcgtgc gtcccgaccg gtggaagctg ccgccgaacc ctgaccccgg
     5341 cgtgtggaag aggatcgggg gcgtggtccg cgagcacgac ccgtactgcg cgggtctgct
     5401 cgtcctcggg caggcggcgg gggaggagga gctcgccgag agcttcgccg cggcggcgtc
     5461 ggagcctctg tgcaacgggt tcgcgatcgg gcgctccatc tacggcgacc cggcgcgccg
     5521 ctggctggcc ggtgagatag gtgacgaaga gctcatctcc tccgtagcag agaggtaccg
     5581 gcgcatgatc tcgctctggc ggagcaggaa cgagccccgg cggggcgcta cggtatgacg
     5641 ccggaagatt cgagttgaag gaggatcggg tggtgaacac cgggaagcgc gcgggcatcg
     5701 agcaggtctc ggtcggggtc gtgggggcag gcggcatggg cggcgtgcac gcccggaacc
     5761 tgcgatcaag gatcgcgggc gctagcctgg ccgccatcgc cgacgtggac gcggaccgcg
     5821 ccgcgagggt agccggcaag aacgtccccg tttttgggga tgccgtggag atgatccggg
     5881 acgacggcgt cgaggccgtc gtgatcgcct cgccgagcgc tacccacgcc gggctgaccc
     5941 tggagtgcct gcgtctcggg aagccggtcc tgtgcgagaa gccgctctct accacggcgg
     6001 aggacgcgct cgaggtcgtc gaggccgagg tcgagctggg cacgaaactg gtccagcttg
     6061 gcttcatgcg ccgcttcgac aggcagcacc tcacggtgaa agaggtcgtg gattcgggct
     6121 ccataggcgc tccattgctc ttcaagggat ggcaccggaa cccgcatatc atgtcctcga
     6181 cggctacctc ggagtccgtc gtgatcgact ctatgatcca cgacctggac tccgtcaggt
     6241 ggatgttcgg gagcgagata gacgaggtct acacgcgcgg gattagcacc aggccggacc
     6301 tcgaaggagc ctgcgatctg cagctgaccc agatatccgt cggtggagga ttgctcgcta
     6361 cggtcgaggt ctacgttacc gccgagtacg gctacgaggt cgggctggag gtggtctgtg
     6421 agtccgggac cgcgcacgtc gctcccttcg acgaagcggt cgtgcgacgc gacgcgaccc
     6481 tgtaccagcg gatccacacg gatccgttcg ccaggttcca gccggcgttt gtggccgaga
     6541 tggaacggtg gacgggggcg atccaaaacg gagagcagcc cgacggcccg gatgcgtggg
     6601 atggatacgc cgcgctcgtg gcggccgtga gttgcataga gtctttgcgc tcgggctcac
     6661 cgcggagcgt gacggtaccg gagcggcccg cgctctacag caaggaggac gcgggggtgg
     6721 tctggtgagg gaagaggcgc gcgtcgcggg ggctccggtg tcgtgggggg tcatcgaggt
     6781 gcctgactgg ggctaccaga tgcccgccga ccgggtgttg cgcgaggcgg cggcgctcgg
     6841 tctggctgcc atcgaggccg gccccgaagg atttctgccg caagaccccg ccgaggtctc
     6901 cgacctcctt gccggatacg gcctgagcct cgtcggaggg ttcgtgcccg cggtgctgca
     6961 cgacgccggc tcgcgcgagg cgcagctggc tttagtcgag aggcgcgcgg agttcttcgc
     7021 ggccgccggg gccgaggtgg tggtcctggc agcggccacg ggctcggaga gttacgagga
     7081 ggtcgccgag ctggacgacg gcgcgtggcg ggagctcttc gagtccctct cgacggtcga
     7141 ggagatctgc gcccggcacg gcctcacgct cgcgctgcac caccactacg gcaccgtgat
     7201 cgagcgcgac gaccagctca ggcgcttcct cgagggttgc gagacggggc tgtgtctgga
     7261 caccggccac ctcgtgctgg gagggagtga tcccgtcgag atcgccgagc tggcggggga
     7321 cagggtgagc cacgtccacc tcaaggacgt ggaccgggac ctcgggcgtc gggtagccac
     7381 gcgagagttg agcttcaagg aggccgccgg ccggggggcg ttccggcccc tgggcgacgg
     7441 tgacgtggat gtcgcagagg tggtagcccg gctcgaacgc tcgggctaca gcgcctggta
     7501 cgtgctcgaa caggacaccg tggtcgagac cgagccggac gaaggcgaag gacccaccgg
     7561 cgacgtccgc aagagcctcg cctacctgct aagacggctc gaaaggaccg cctgaaccgg
     7621 agaggaggag atcacttgga cacgatacgt ctgactacag cgcaggcgct ggtccggttc
     7681 ctggccaacc agtacgtcga gcgtgatggg gtggagcaac gcctgttcgc cggtgccttc
     7741 ggtatcttcg gacacggcaa cgttgccggc gtcggacagg cgctccatca gtatggggac
     7801 gagctcacct actaccagcc gcgcaacgag caggcgatgg ttcacaccgc cgccgcctac
     7861 gcccggcaga agaaccggct ccagacgctg gtctgcacct cctccatcgg tcctggagca
     7921 accaacatgg tcacgggagc ggcgcttgcc accattaacc gcctgccggt cctcctgctg
     7981 cccggcgacg tcttcgcctc gcgcgcgccg gacccggtcc tgcagcagct ggaggcgccg
     8041 tacgacggca ccctctcggt caacgactgc ttcaggcccg tctcgaagta ctgggaccgc
     8101 atagaccgcc ccgagcagct catccccgcg gctctagctg ccgtgcgggt cctgaccaac
     8161 caggccgaga ccggagccgt gaccctggcg ctgccccagg acgtgcaggc cgaagcgtac
     8221 gatttccccg aggagttctt cgagacgcgg gtgtggcacg tgcctcggca gctgccc
//
LOCUS       DASTUV010000035         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_389153, whole genome shotgun sequence.
ACCESSION   DASTUV010000035 DASTUV010000000
VERSION     DASTUV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389153"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFT03_00700"
     CDS             complement(<1..476)
                     /locus_tag="VFT03_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4846689.1"
                     /translation="MADQFDLVVIGGGNAGYIPAIRASQLGMSVALIEKREGGHLGGT
                     CLNLGCIPTKALLQTAHLLHDARNGEEYGVKVGEVEFDYPQAAKRREQVVSQLRRGVQ
                     GLMKKNKVTVYNGVGSFVEPKKIKVETEDGEEELEAKNVLISTGSAVNTLPGLEFD"
     gene            complement(498..1358)
                     /gene="mutM"
                     /locus_tag="VFT03_00705"
     CDS             complement(498..1358)
                     /gene="mutM"
                     /locus_tag="VFT03_00705"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU4846690.1"
                     /translation="MPELPEVETKKEDLRELVAGSVIERARVIDSSLVEQPSAEEFVS
                     RLEGVRITGARRRAKHLIVELGSGDSLVFQLKIGGQLLLVPPVEEPETALMLVLDLDG
                     ERRLFLRDETGFTRARLLDADELQERLSPLGPEPLEEEFDAGYLQRAIGSRRAQIKPL
                     LLDQKVVSGIGNIYADEILYDARLHPRRKANTLSEGEWEALHAAIRSNLAAGVEHRGT
                     TVRLYKDVLDRPGEHQNYLRVFEKHGRPCPGCRGEVVREKVSGRPTHFCPACQREDSP
                     GGGGRLESLW"
     gene            complement(1402..2052)
                     /locus_tag="VFT03_00710"
     CDS             complement(1402..2052)
                     /locus_tag="VFT03_00710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4846691.1"
                     /translation="MEILDRNGIHDGLVVDLGCGSGLWARELVDAGYRVLGIDISDPM
                     IELSRNRVPEAEFSVGSLFEAEIPRCQAVTAVSEVLNYLFDAENEERGLGRLCRRVHD
                     ALVPGGVFVFDVLGPGQVPPGSTVRGFRVGEDWAVFFEREEDFERGTMERRIVSFRKV
                     GECYRRDEEVHRVRLYDPSGLSAELQQAGFRVRTMRSYGAHHLSEGHAAFVARKPA"
     gene            complement(2159..2893)
                     /locus_tag="VFT03_00715"
     CDS             complement(2159..2893)
                     /locus_tag="VFT03_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEU4846692.1"
                     /translation="MNPEEFLQRLKREVIGHPALTHPFLERFGEGDADAEGIRTFAIQ
                     YYRHVRVSRLYLAALISNCREDEKLQLALAEVLFDEYGHLDPEETHPALYRRFLEALG
                     IGEEEWEEPPTLPEIEMYITTHYALSGHPDFRLGLGALGPASEWPVPPIYARLSDGLR
                     KAAGLPDEALEIFTSHVTMDVTHARIMMEALAPYAGDEEGQRRVHEGAMRSLDARSVM
                     LGGLYRAVYREPVPLFDAPSVRLTGD"
     gene            3112..3642
                     /locus_tag="VFT03_00720"
     CDS             3112..3642
                     /locus_tag="VFT03_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846693.1"
                     /translation="MTDTKNNFRQDWLSVPRRREEERLDPHVYKRGINGLTVEEALRR
                     AFPRVIDILGRSGEQYSEFELQLVRQTVRACLISQVSRANLYRIAGILVADRRDEGAH
                     IDTSLALAYVAALREAFLTELSVGERVQTPLGSGEVRYVSGDGANAAVKLDSDYEVWL
                     PAERVSRPNPLVRRAS"
BASE COUNT          685 a         1256 c         1107 g          746 t
ORIGIN      
        1 tgatcgaact ccaggcctgg cagggtgttg acggccgagc cggtggagat caggacgttc
       61 ttcgcttcga gctcctcctc gccgtcttcg gtctctacct ttatcttctt gggctccacg
      121 aagctgccta caccgttgta aacggtgacc ttgttcttct tcatcaggcc ctgcacgccg
      181 cgtctgagct ggctgacgac ctgttcgcga cgcttggctg cctgcgggta gtcgaactcc
      241 acctcgccga ctttgacccc gtactcttcg ccgtttctgg cgtcgtgcaa cagatgggcc
      301 gtctggagca aggctttggt cgggatgcag cccagattca ggcacgtccc tccgagatgc
      361 ccgccctccc gcttctcgat gagggctacg ctcattccca gctggctggc ccggatcgcc
      421 ggtatgtatc ctgcgttccc accaccgatt actacgaggt cgaactgatc agccaccctt
      481 ggctcctttc acgtctacta ccacaacgat tctaaccttc cccctcctcc ggggctatcc
      541 tcccgctggc aagcaggaca gaagtgcgtt ggtctcccac ttaccttttc ccgaaccacc
      601 tctcctctac accccggaca cggccgcccg tgcttctcga agacccgcag gtagttctga
      661 tgctcgcccg gccggtccaa tacatccttg tagagccgca cagtggtgcc ccgatgctct
      721 acgccggccg ccaggttgga ccggatcgcc gcgtgcagcg cctcccattc gccctccgaa
      781 agggtgtttg ccttgcgcct cggatgcagc ctcgcatcgt acaagatctc atcggcgtag
      841 atgttcccta tgcctgagac gactttctga tccaggagca ggggctttat ctgggccctc
      901 cgcgaaccga tggcacgttg cagatatccc gcgtcgaact cttcttctag gggctctggt
      961 cccagcgggg agagccgctc ctggagttcg tccgcgtcga gcaggcgcgc ccgcgtgaag
     1021 cccgtctcgt cgcgcaggaa gagcctcctc tcgccgtcca gatccaggac gagcatgagc
     1081 gcagtctcag gctcctcgac gggcggcacg agcaggagct ggcccccgat cttgagctgg
     1141 aacaccagcg agtcccctga gccaagttcc acgatgaggt gcttggcccg ccgccgcgcc
     1201 cctgtgattc ggacaccttc gagccggctt acgaactctt ccgccgaagg ctgctccaca
     1261 agcgaggaat cgatgaccct ggcccgctct atcacagagc cggcgaccag ctcgcgcagg
     1321 tcttcttttt tggtctcgac ctctggcaac tccggcatgc gcatatttat acccgctcga
     1381 cgctgcccta ttcctcctgc ctcatgccgg tttgcgcgcc acgaaggcgg cgtggccctc
     1441 tgacagatga tgggcaccgt aggatctcat cgtccgaacc cgaaagcccg cctgctggag
     1501 ttctgcggac aaccccgacg ggtcgtagag ccgcacgcgg tgcacctctt catcccgcct
     1561 atagcactcc cccaccttgc ggaaactcac gatccggcgc tccatcgtac cccgctcgaa
     1621 gtcctcctcc cgctcgaaga agaccgccca gtcctccccc acgcggaaac ccctcaccgt
     1681 gctaccgggc ggcacctgcc caggaccaag cacgtcgaag acgaagacgc cgccggggac
     1741 gagcgcgtcg tgaacgcgcc ggcaaagacg accaagcccc cgttcctcgt tctccgcatc
     1801 gaataagtag ttgaggacct cgctgacggc cgtgacggcc tgacaacgtg ggatctcggc
     1861 ctcgaacagc gaccctacgc tgaactcggc ctcgggcact ctattgcgcg agagctcgat
     1921 catagggtcg gagatgtcga tccccaggac cctgtagcca gcgtctacca gctcccgagc
     1981 ccacagaccg ctaccgcaac cgaggtcgac aacgaggcca tcgtgtatcc cgttgcggtc
     2041 caggatctcc attatgcccg gcgccgctcc gagggcgaac tcagcatggc cgacatcatg
     2101 tatgtacgca aggtcctccc cgtaccaccg cgtcgtatcg ctgccttccg acgttatctc
     2161 agtctcctgt aagccggacg gagggagcgt cgaacagggg gacgggttcc ctgtagacgg
     2221 ccctgtatag accaccgagc ataacggagc gggcgtccag cgagcgcatg gcgccctcat
     2281 ggactcttcg ttggccttcc tcgtcccctg cgtatggtgc gagcgcctcc atcattatgc
     2341 gggcgtgggt cacgtccata gtgacgtggc tcgtgaagat ctccagcgct tcgtcaggaa
     2401 ggccggccgc ctttctgagg ccgtcggaaa ggcgggcgta gatcgggggc acgggccact
     2461 cgctggccgg ccccagagca ccgagcccga gcctgaaatc tggatggccc gagagcgcat
     2521 agtgggtggt gatgtacatc tctatctcgg gcagcgtcgg aggttcctcc cactcctcct
     2581 caccgatgcc aagcgcctcc aggaaacgcc tgtacagggc tggatgcgtc tcttcggggt
     2641 cgaggtgacc gtactcgtcg aagagaacct cggcgagcgc gagctggagc ttctcatcct
     2701 cgcggcagtt cgagataagg gcggccaggt acaaccggct caccctgaca tgcctgtagt
     2761 actggatcgc aaacgtcctt atcccctccg catcagcatc cccctcccca aaccgctcca
     2821 gaaaagggtg cgtgagcgca ggatggccga tgacttcacg ttttagtcgc tgaagaaact
     2881 cctccggatt cacctgtctc cttccagaga actccctaca gtttagcgca aggttcgagc
     2941 tggtcagcac gtcgcccggg acaggcaacg tgtcagctct tcagccggtc agcttttgtg
     3001 cttgcgagcc ctggctgaca cgcgaagccc gaaggacgta gcgtgctgaa atgctgattt
     3061 tacaggcaat ttattgttgt ctcactagtc tcagtgaggt ataatagcga catgacagac
     3121 acgaagaaca acttcaggca ggattggctc agtgtcccgc ggcggcggga ggaggagcgt
     3181 ctggacccgc acgtgtacaa gcgcggcatc aacgggctga cggtcgagga ggcgttacgt
     3241 cgggcgtttc cgagggtgat agacattctg gggaggagtg gcgagcagta ctcggagttc
     3301 gagctgcagt tggtaaggca gactgtcagg gcttgtctga tctcccaggt ttcccgcgcg
     3361 aacctctacc gtatcgccgg catcctggtt gccgacaggc gcgatgaggg tgcgcacatc
     3421 gatacttcgc tcgcgctggc ctacgttgcg gcgctcaggg aggcattcct cacggagctc
     3481 tccgtcggcg agcgcgtaca gacgccactc ggttccggcg aggtccgcta tgtctctggt
     3541 gacggcgcga acgccgcggt caagctagac agcgactacg aagtctggct gccggctgag
     3601 agggtgtcgc gacccaaccc gctggttcgg cgcgcttcct agggagagac tgtcatagac
     3661 aggagctttc tgggtagact aggagggtcg taaaggctac agagggcgcg ccatgcagaa
     3721 gtcaagagca cgggtaatct gcaacccgac ctccggtggc ggtgtctacg acccccatga
     3781 gatccgcgac gagc
//
LOCUS       DASTUV010000036         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_463307, whole genome shotgun sequence.
ACCESSION   DASTUV010000036 DASTUV010000000
VERSION     DASTUV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463307"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..762)
                     /locus_tag="VFT03_00725"
     CDS             complement(31..762)
                     /locus_tag="VFT03_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU4846694.1"
                     /translation="MGEAGSGGRKLLIVGAHSGDFVWRAAGAIAVATSRGGEARVVAL
                     SYGERGESGDLWDEPDQTVENVKRVRHAQSEEAARAIGARFECWDLGDYPLRADEETV
                     ERLADEIRGFAPDVILTHTERDPFNPDHPVAHAAVLRARLLASGAGVASAFETIKPPE
                     LLYFEPHQPELCGFQPNTFVDITGVFERKLKAMEAMEAQKYMHKHYAERAEHRAFHAR
                     YATGDGEVRYAEAFQRAIPQVVRSL"
     gene            complement(954..1026)
                     /locus_tag="VFT03_00730"
     tRNA            complement(954..1026)
                     /locus_tag="VFT03_00730"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(990..992),aa:Glu,seq:ctc)
     gene            complement(1125..2615)
                     /gene="gltX"
                     /locus_tag="VFT03_00735"
     CDS             complement(1125..2615)
                     /gene="gltX"
                     /locus_tag="VFT03_00735"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU4846695.1"
                     /translation="MENQEIRVRYAPSPTGRLHVGGVRTALFNWLFARKNGGVFALRI
                     EDTDLERSTEESVEQLERSLRWIGLEWDEGPEVGGPHSPYRQTERMELYREAAQRLLE
                     SGAAYYDFATPGELKEFRERAQAEKRQPIYRGGEYRDMDPGEARRKVDAGKPHTVRFK
                     TPREGQTVVEDMIRGPVTFDNANLEDFVLMKSTNTPTYNFAAAVDDAEMEISHVIRGD
                     DHLSNTPRQILLYEALGLGLPAFAHVPQVLGPDRKKLSKRHGAASVEDFAEQGILPEA
                     LFNYLALLGAGYAADEEIFTPEELAKRFRIDRVSGNPAVFDENKLLSINQVYIRRKSP
                     EELAMLAAPMLAEAGVARPDELERDMPRLTQIMDLLKERISRTTDIPDAVGYFYGGTL
                     DYDPDEFEKQFGKDFVRENLPELYERLAALTEWTEEAIEEAVRGLAAEKEKGARHLIH
                     PLRFATTGRTVSAGLFETLQLLGRERSLLRIEDVLEKLQRLSKPVS"
     gene            complement(2644..3330)
                     /locus_tag="VFT03_00740"
     CDS             complement(2644..3330)
                     /locus_tag="VFT03_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEU4846696.1"
                     /translation="MATARETILWRIRRATRDVPEGEQPEDDTVERGYRRKDDSPREE
                     IVERFAENVAEYEATVRRVASAELPGAIEEALARRGVKRLVIPPYLPKEWVPEGIEAL
                     RDAARPRLSNEELDASDGVLTGCALGIAQTGTIVLDSGRAQGRRALTLLPDYHLCVVR
                     EDQIVGLVPEAFAKLEETVKNEGRPITFISGPSATSDIELDRVEGVHGPRTLEVLVAG
                     LSARRFAPAF"
     gene            complement(3330..>4196)
                     /locus_tag="VFT03_00745"
     CDS             complement(3330..>4196)
                     /locus_tag="VFT03_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEU4846697.1"
                     /translation="YLREKFLSAKVGISGANFAVAETGTVCVVESEGNGRMCTTLPQV
                     LITLMGIEKLIPAWRDFEVFMQLLPRSSTAERMNPYTSFWTGVREGDGPQEFHLVLLD
                     NGRTDVLEDEVGRQALNCIRCSACLNVCPVYERTGGHAYNSVYPGPIGAILTPQLVGI
                     GKRGPDSLPYASSLCGACYEVCPVKINIPEVLIHLRGRVVRRRQDTGGPRAKLDPENA
                     AMQLLAKVFSDRRRFEGAQKLAKLGQGPLARGGEIRRLPGKLAGWTDVRDLKPVPDQT
                     FREWWKANRGGA"
BASE COUNT          637 a         1405 c         1375 g          779 t
ORIGIN      
        1 gggaggcgtc cgccggccgc tcctctgggc tcacaatgac ctcaccacct gcggaatcgc
       61 acgctggaac gcctcggcgt agcgaacctc gccgtccccc gtggcgtatc tcgcgtggaa
      121 ggcgcggtgc tctgccctct cggcgtagtg cttgtgcatg tacttctggg cctccatcgc
      181 ctccatcgcc ttgagcttgc gctcgaagac gccggtgatg tccacgaacg tgttcggttg
      241 gaagccgcag agctccggct ggtgcggctc gaagtagagt agctccggcg gtttgatggt
      301 ctcgaaggcg ctcgccaccc ccgccccgct ggccaaaagc cttgcgcgca gcacggcggc
      361 gtgggcaacc gggtggtccg ggttgaacgg gtcgcgctcg gtgtgggtca gtatcacgtc
      421 cggggcgaac ccccggatct cgtcggccag ccgctcgacc gtctcttcgt cagccctcaa
      481 ggggtagtca ccgaggtccc agcactcgaa acgggcccct atcgccctgg cagcctcctc
      541 cgactgcgcg tgtctgaccc tcttcacatt ctcgaccgtc tggtccggct cgtcccaaag
      601 gtctccggac tcgccgcgtt cgccgtacga gagcgccacc acccgtgcct ccccgccccg
      661 cgacgtcgcg accgcgatcg cacccgccgc ccgccacacg aagtcgcctg aatgtgcccc
      721 cacgatcagt agcttcctgc cgccgctacc agcctcgccc atctgctcag ccttcctttt
      781 tccgcccgcg tcctcgagcc cgcctgaaga ccgttggcgc acgatgataa cgtacttgga
      841 cttgacccgg tccgccaccc acgcgatgat tcgaccctaa cgaacgtcgg cgagttgaca
      901 actcaggtgt gtgtgtaggc tgcgtcggga cggccgttat accgcccgag aagtgacccc
      961 accgggattc gaacccgggt tttcgccgtg agagggcgat gtccttggcc tctagacgat
     1021 ggggccttgc cgaaaggcga ggatagattc tagtccatga cccccatttc gtcaaagcgc
     1081 tgtaggcatc tggccacagc gcccagtctt actgaagtct ctcgttagct gactggcttc
     1141 gagagccgct gcaacttttc gaggacgtct tcgatcctca gcaggctgcg ctcgcgcccg
     1201 agcaactgca gggtctcgaa gagccctgcg ctgacggtgc gtccagtggt ggcgaagcgc
     1261 aaggggtgta tgaggtgccg cgcgcccttc tccttctccg ccgcgaggcc gcgcacggcc
     1321 tcttctatgg cctcttctgt ccattccgta agcgccgcga gccgctcgta gagctcgggg
     1381 aggttctcgc ggacgaagtc cttgccgaac tgcttctcga actcgtccgg gtcgtagtcg
     1441 agcgtgccgc cgtagaagta accgacggcg tccgggatgt cggtcgtgcg ggagatgcgc
     1501 tccttcaaga ggtccatgat ctgcgtgagg cggggcatgt cgcgctccag ctcatccggc
     1561 ctcgcgaccc ccgcctcggc gagcatgggg gctgcgagca tcgcgagctc ctccgggctc
     1621 ttgcggcgga tgtagacctg gtttatcgag aggagcttgt tctcgtcgaa gaccgcgggg
     1681 ttaccactca cacggtctat gcggaagcgc tttgccagct cctcgggggt gaagatctcc
     1741 tcgtccgccg cgtagccggc cccgaggagc gcaaggtagt tgaacagcgc ctcgggcagg
     1801 atcccctgct cggcgaaatc ttcgacgctg gccgcgccgt ggcgcttgga cagcttcttc
     1861 ctgtcagggc cgagaacctg cgggacgtgg gcgaaggcgg gaagcccgag tccgagagct
     1921 tcgtagagca ggatctggcg cggcgtgttg gagaggtggt cgtcgcccct tatgacgtgg
     1981 gagatctcca tctccgcgtc gtccacggcg gcggcgaagt tgtaggtcgg ggtgttggta
     2041 gatttcatga ggacgaagtc ctccaggttg gcgttgtcga aggtgacggg gccccggatc
     2101 atgtcctcca ccaccgtctg gccctcgcgc ggggtcttga accggacggt gtgcggcttc
     2161 ccggcatcta cctttcttcg cgcctcccca gggtccatgt cgcggtactc gccacccctg
     2221 tagatcggct gccgcttttc ggcctgcgcc cgctcccgaa actccttcaa ctccccaggc
     2281 gtagcgaagt cgtagtacgc agcgcccgac tccaggagcc tctgcgccgc ctcgcggtaa
     2341 agttccatac gctcggtctg gcggtagggg gagtgcgggc cgccgacctc agggccctcg
     2401 tcccactcca ggccgatcca gcgcagagag cgctcgagct gctcgacgga ctcctccgtc
     2461 gaacgctcca ggtccgtgtc ctcgattcgc agcgcgaaga cgccgccgtt cttgcgggcg
     2521 aagagccagt tgaagagagc tgtcctcacg ccgcctacgt gcaacctccc cgtggggctc
     2581 ggcgcgtagc ggaccctgat ctcctgattc tccattccta gaaactagca cacggtccgt
     2641 aggttagaaa gccggcgcga agcgccgtgc tgacaagcct gcgactagga cttcgagggt
     2701 acggggaccg tgcacgccct cgacgcggtc gagctcgata tccgaggttg cggacgggcc
     2761 ggagataaag gtgatgggac gcccttcgtt cttcacggtc tcttccagtt tcgcgaaagc
     2821 ttcggggact aggcccacga tctggtcctc cctcaccacg cagaggtggt agtcggggag
     2881 gagggtgagt gccctgcggc cctgcgccct acccgagtcg aggacgatgg tgccggtctg
     2941 ggcgatgcca agggcgcagc ccgtcagcac gccgtcgctg gcgtcgagct cctcgttgga
     3001 gaggcgtggc cttgccgcgt cgcgcagcgc ttctataccc tcggggaccc actccttcgg
     3061 gaggtaaggg gggatgacga ggcgcttcac gccgcgccgc gccagagctt cttctatcgc
     3121 gccggggagc tcggccgacg ccacgcggcg gacggtggcc tcgtactcgg caacgttctc
     3181 ggcgaagcgc tcgacgatct cctcgcgagg ggaatcgtct ttcctgcggt agccgcgctc
     3241 cacggtatcg tcttcaggct gttccccttc ggggacgtcg cgggtggcgc ggcggatgcg
     3301 ccagaggatc gtctctctgg cggtcgccat caggctcctc cccggttcgc cttccaccac
     3361 tcgcggaagg tctggtcagg gacgggcttc aggtcgcgca cgtcggtcca gccggcgagc
     3421 ttgccgggga ggcgccggat ctcgcctccc cgcgccagcg gtccctggcc cagcttcgcg
     3481 agcttctggg ccccttcgaa gcgccggcgg tccgagaaga ccttcgccag gagctgcatc
     3541 gcggcgttct cggggtcgag cttcgcgcgc ggtcctccgg tgtcctggcg gcggcgcacc
     3601 acgcggccgc gcaggtggat caggacctcg gggatgttga tcttgaccgg gcacacctcg
     3661 tagcaggccc cgcagagtga ggaggcatac ggcaaggaat caggcccccg cttgccgata
     3721 ccgactagct gcggcgtgag gatggcgcct atggggccgg gatagaccga gttgtaggcg
     3781 tgaccgccgg tccgctcgta gacggggcag acgttgaggc acgccgagca gcggatacag
     3841 ttgagagcct ggcgcccgac ctcgtcttct agcacgtccg tgcggccgtt gtctaggagg
     3901 acgaggtgga actcctgcgg accgtcgccc tccctgaccc ctgtccagaa cgaggtgtac
     3961 gggttcatcc gctccgcggt agaggagcgg gggaggagct gcatgaagac ctcgaagtca
     4021 cgccaggccg ggatgagctt ctcgataccc atgagcgtaa taagaacttg cgggagcgtc
     4081 gtgcacatcc ggccgttccc ctcggactcg acgacgcaca cggtccccgt ctcggcgacg
     4141 gcgaagttcg cccccgagat gccgaccttc gcggagagga acttctcgcg caggta
//
LOCUS       DASTUV010000037         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_174094, whole genome shotgun sequence.
ACCESSION   DASTUV010000037 DASTUV010000000
VERSION     DASTUV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174094"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..682)
                     /locus_tag="VFT03_00750"
     CDS             complement(44..682)
                     /locus_tag="VFT03_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU4846698.1"
                     /translation="MPEFRGISAILLAAGSGSRFGGGKLLAPYRGRPLIEASLANLAE
                     APIEETVAVVGDDAGRLRPVCEPYGVRVVENPDWRQGQSTSVRAGLLALGPEVRAAVV
                     LLADQPLVGPGAVGRLVAAFEEGAEVAVATYGGRRRNPVLFSRAVWPLLEAELSGDEG
                     ARAVLRRHPDLATEVPCDGVGDPADVDTEEDLRSLERMREREHHSKMGKKRA"
     gene            complement(675..1277)
                     /locus_tag="VFT03_00755"
     CDS             complement(675..1277)
                     /locus_tag="VFT03_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU4846699.1"
                     /translation="MGGDLVGVVLGAGRSSRFGTPKQLLPFGDTTMLGQVVCNANTSD
                     LDRVVVVLGRASEELRGSVDFGRAVVVDNTAYGTGCASSLLAGMDAAGEDCEALALLL
                     GDQPGVRAELIDRALSEWRRERPWASVTSYEGKLGHPFVFAREAFEDLRGLHGDKAVW
                     KLIEEYPERVRRIEIDAPLPPDVDTPEDYELALSHWRSGA"
     gene            complement(1277..>2189)
                     /locus_tag="VFT03_00760"
     CDS             complement(1277..>2189)
                     /locus_tag="VFT03_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU4846700.1"
                     /translation="GELAQAERPFAVVTVVRTEPSTSARPGNRALVFPDGSMEGWVGG
                     GCIQPTARREGLSALEEGEPRLVRITPDTAASQHNVHMARMTCASEGTADLYVEPFLP
                     RPTLAAAGDSPLTTTLAAIAPPLGFRFLAIDAATGLNPEAIPHPRDTWLVVATFGEFD
                     EDAVEAGVRLSLPYVGLVASERRAGDILSELRARGLEDGDLSVVRSPAGIPIGASGQE
                     EISLSIMAEIVSLRAKLRPGLREEPATQPASQPRDATDPVCGMTVEVEGARHVARYGG
                     RDYYFCCPGCKRRFEAEPEKYVSPISA"
BASE COUNT          331 a          804 c          720 g          334 t
ORIGIN      
        1 tttatcttca tcgagtctcc tccttgaagc gcaggagttg cgcttacgct ctcttcttac
       61 ccatcttaga gtggtgttcc cgctcgcgca tacgctctaa actcctcagg tcctcctcag
      121 tgtctacgtc cgccgggtcc cccaccccgt cacaggggac ctccgtcgcc aggtccggat
      181 gccgccgcaa gaccgcccgc gcgccctcgt cccccgagag ctctgcctcc agcagcggcc
      241 acactgcccg ggagaacagc acggggttgc gccgtctccc gccgtaggtg gctaccgcca
      301 cctccgcccc ttcctcgaac gccgccacca ggcgtcccac ggcccccggt cccacgagcg
      361 gctggtcggc gagcaggact acagccgccc gaacctccgg ccccagagcc aggagcccgg
      421 cccgcaccga ggtggactgg ccctgccgcc agtctgggtt ctccacgacg cggacgccgt
      481 aaggctcgca gacggggcgc agcctccccg cgtcgtctcc gacgacggca accgtttctt
      541 ctatcggagc ctccgcgagg ttcgcgaggg aggcctcgat caggggcctc cccctgtacg
      601 gcgcgaggag ctttccgccg ccgaaccggc tcccggaccc tgcggcgagc aggatggccg
      661 agatcccgcg gaactcaggc accgctccgc cagtgggaga gggccagctc gtagtcttcc
      721 ggtgtgtcca catcgggagg cagcggcgca tcgatttcga tccggcgcac cctctccggg
      781 tattcctcga tgagcttcca cacggccttg tccccgtgca gaccgcgcag gtcctcgaag
      841 gcttccctgg cgaagacgaa cgggtgcccg agcttccctt cgtaagacgt cacggaggcc
      901 cagggccgtt cccggcgcca ttcggagagc gcgcggtcta tgagctccgc tctcacgccc
      961 ggctggtcgc ccaggagcag cgcgagagcc tcgcagtcct cgcccgccgc gtccatcccg
     1021 gcgagcagcg aggaggcgca gcccgtgccg taggcggtgt tatcgaccac cacggcccgc
     1081 ccgaagtcca ccgagccgcg cagctcctcc gacgcgcggc caaggacgac gaccacccgg
     1141 tccaggtccg aggtgttggc gttgcaaacg acctgcccga gcatcgtggt gtcgccgaaa
     1201 ggcagaagct gcttgggagt gccgaaccgg ctcgaccgac cggctccgag caccaccccg
     1261 acgagatcgc cgcccatcag gcggagatcg gggagacgta cttctcgggc tccgcctcga
     1321 acctgcgctt gcacccaggg cagcagaaat agtagtccct gcctccgtaa cgggcgacgt
     1381 gccgggcgcc ctctacctcg actgtcatgc cgcagacggg gtctgtggcg tcccgcggct
     1441 gagaggctgg ctgtgtagcg ggctcttccc ggaggccggg gcggagcttc gctctcaaag
     1501 agacgatctc ggccatgatg gataacgaga tctcctcctg cccgctcgcc ccgatcggta
     1561 tgcccgccgg cgagcgcacc accgaaaggt ccccgtcctc gagcccccgc gcccgcagct
     1621 ccgacagaat atcgccggcc cgccgctcgc tcgcgacgag ccccacgtag gggaggctca
     1681 gccgtacccc ggcctcgacc gcgtcctcgt cgaactcccc gaaggtggcg actaccagcc
     1741 aggtgtctcg tggatgggga atcgcctcgg ggttaagtcc cgtagcggcg tctatagcga
     1801 ggaaccggaa gcccaggggc ggcgctatag cggcgagcgt ggtcgtgagc ggcgagtcgc
     1861 ccgcggcggc gagcgtgggg cgaggcagga aaggctcgac gtagaggtcc gccgtgccct
     1921 cgctggcgca ggtcatgcgg gccatgtgga cgttatgctg ggatgcggcg gtgtccgggg
     1981 tgatcctgac gagcctgggc tcgccttctt cgagagcgga gagcccttcc ctgcgggcgg
     2041 tgggctgtat gcacccgccg ccgacccatc cctccatgct cccgtccggg aagacgaggg
     2101 cccggttgcc agggcgggcg gaggtggagg gctcggtgcg aaccaccgtt acgacggcga
     2161 agggacgctc cgcctgcgcg agctcgccc
//
LOCUS       DASTUV010000038         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1746941, whole genome shotgun sequence.
ACCESSION   DASTUV010000038 DASTUV010000000
VERSION     DASTUV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746941"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFT03_00765"
     CDS             complement(<1..404)
                     /locus_tag="VFT03_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEU4846701.1"
                     /translation="MAQNGSSGGQRAKRVAIRAHPWIERLARFGYIAYGVVYALVGVL
                     AVQAAFSGADQAAGQQGALRSVLVAPLGRILLCIIALGLLAYAAWRLYEGVMDPEHEA
                     RGATGIVKRLDHVLNGLFHGALAFTAGHLALGS"
     gene            544..2286
                     /locus_tag="VFT03_00770"
     CDS             544..2286
                     /locus_tag="VFT03_00770"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU4846702.1"
                     /translation="MRVALAQINTTVGDVWGNAEKAASALERATEAGAQLVALPELTI
                     TGYPPEDLLMRPSFIKDNLRALEEFAGEVPAGVVAAIGFVDLDTDLHNACAVISGGEV
                     LHRYHKRFLPNYGVFDENRYFREGSGAPILRFDGALVGVSICEDIWYPGGPAREQALG
                     GAGVILNISASPYHRLKGAFRERMLAVRASDYGCYVVFCNLVGGQDELVFDGHSLVFD
                     PEGDLISRASQFEEDLLVVDLYPEEVLTQRLHESRPRKESAKHPPEIVSVPGFEERTG
                     PVRPRDHRVEPLFREEGEVFEALVLGLRDYFQKNGFSRAVLGLSGGVDSSLCAVVASR
                     ALGPQNVTGVLMPSRYTSEASNTDAEAVAKNLGIDTQVVPIGPAFDAYMEMLEGPFKS
                     LPEDTTEENLQARIRGNIIMAFSNKFGWIALATGNKSELSVGYATLYGDMAGGFAVIK
                     DVPKTLVYRICRHVNETEGREVVPESVLTKEPSAELKPGQRDVDSLPPYEILDPILEA
                     YIEEDKGVREIVARGFDEEDVRRVIKLVDAAEYKRRQAPIGIKVTSRAFGRDRRMPIT
                     NRYSERASGETDAR"
BASE COUNT          482 a          873 c          826 g          438 t
ORIGIN      
        1 gagccaagcg ccaggtgccc ggcggtaaag gcgagagcac cgtgaaacag cccgttgagc
       61 acgtggtcga gacgcttcac gatccccgtc gcgcccctgg cctcgtgctc gggatccatt
      121 accccttcgt agaggcgcca cgccgcatac gcgaggaggc cgagggcgat aatgcagagc
      181 agtatcctac ccagcggcgc gacgaggacc gagcgcagcg cgccctgctg gccggcggcc
      241 tgatcggcgc cgctgaacgc tgcctgtaca gccagtaccc ctaccagcgc gtagaccacg
      301 ccgtaggcta tgtagccgaa ccgcgcgagg cgttcgatcc aggggtgcgc cctaatcgcc
      361 accctcttgg cgcgctgccc gcccgacgaa ccgttctgtg ccacgcctcg ttcctcctga
      421 ccgtgtggca gctgccacac actctatacc cccgccacgg gggtttggac gaagctatgt
      481 ttaccatggc gggcgacgca ccggatgtat cgcgaccacc atcgacgcgg tatatttatt
      541 gtcgtgaggg ttgcgctggc gcagatcaac actaccgtcg gcgacgtgtg ggggaacgcc
      601 gagaaggccg cgagcgccct ggagagagca accgaggccg gggcgcagct cgtcgcgctg
      661 cccgagctca ccatcacggg ctacccgccc gaagacctcc tcatgcggcc aagcttcata
      721 aaggacaacc tgcgggcgct cgaggagttc gccggagagg tccccgctgg cgtcgtcgcc
      781 gccatagggt tcgtcgacct cgacacggac ctgcacaacg cctgcgccgt catctccggc
      841 ggcgaggtcc ttcaccgcta ccacaaacga tttctgccga actacggcgt cttcgacgag
      901 aaccgctact tccgcgaagg ctcgggcgcc cccatcctga ggttcgatgg cgccctcgtc
      961 ggggtgagca tctgcgagga catctggtac cctggcgggc ccgcgcgcga gcaggctctg
     1021 ggcggggcag gtgtgatctt gaacatctcg gcctctccct accaccggct caagggcgcc
     1081 ttcagggagc ggatgcttgc ggtgcgggcc tccgactacg gttgctacgt ggtcttctgc
     1141 aacctggtgg gcgggcagga cgagctcgtc ttcgacgggc actcgctcgt attcgacccc
     1201 gagggggatc tcatctcccg cgctagccag ttcgaggagg acctgctcgt agtggaccta
     1261 tacccggaag aggtcctgac gcagcgcctc cacgagtccc ggccccgcaa agagagcgcc
     1321 aagcatccac cagagatcgt gagcgtgcct ggctttgagg agagaaccgg cccggtgcgg
     1381 cccagggacc acagggtcga gcccctattc cgcgaagagg gtgaggtgtt cgaggccctt
     1441 gtgctcggct tgcgcgacta cttccagaag aacggcttct cgcgagcggt gctcgggctc
     1501 tcgggcggcg tggactcttc gctttgcgca gtcgtcgcct cccgcgctct aggcccgcag
     1561 aacgtcacag gcgtcctcat gccgagccgc tacacctcgg aggcctccaa caccgacgcc
     1621 gaggccgtcg ccaagaacct tggcatagac acgcaggtgg tccctatcgg cccggcgttc
     1681 gacgcctaca tggagatgct cgaaggcccc ttcaagagct tgcccgagga cactaccgag
     1741 gagaacctcc aggcgcgcat ccgggggaat atcatcatgg cgttctccaa caagttcggc
     1801 tggatcgccc tggctaccgg caacaagagc gagctgagcg tcggctacgc caccctctac
     1861 ggggatatgg ccggcgggtt cgccgtcata aaggacgtcc ccaagaccct cgtctaccgc
     1921 atctgccgcc acgtgaacga gaccgaaggg cgcgaggtgg ttcccgagtc ggtcctcacg
     1981 aaggaaccct cggccgagct caaaccggga cagcgcgacg tcgattctct gccgccgtac
     2041 gagatcctcg accccatcct cgaggcgtac atagaggagg acaagggggt gcgcgagatc
     2101 gtggcccgcg gcttcgacga ggaggacgtc aggcgcgtca tcaagctggt agacgccgct
     2161 gagtacaagc gacgccaggc gcccatcggc atcaaggtca ccagccgcgc cttcggccgc
     2221 gaccgccgca tgcccataac caaccgctac tccgagcgcg cctccggcga gaccgacgcc
     2281 cgctaggtgt ggacgccgac ctggctgtcg ccgggacccg gccacggcgc ctacgagagt
     2341 tcctccgcgg ggacccatga ctcgaccctc ggtcccgctt tactagcgcc ttacctgcag
     2401 gagcagttgc caggcccatt cggttatctt gagtggctaa agtcgagcag agagaggtag
     2461 cctgaaattt ctcgatgggg tccaaagtcg tgcttgggtc cgtacgacgt acgcgattgc
     2521 atacttcgga taatcctctt gtcgaagctg attagacgag gtctgattag ttgagatcca
     2581 cagagggtat atgaagaagc gtgaagttgt agtgcaccc
//
LOCUS       DASTUV010000039         8641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_352684, whole genome shotgun sequence.
ACCESSION   DASTUV010000039 DASTUV010000000
VERSION     DASTUV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352684"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..460)
                     /locus_tag="VFT03_00775"
     CDS             complement(197..460)
                     /locus_tag="VFT03_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846703.1"
                     /translation="MWLVGAASPLVHSPAVMMLGRRTSNVLLAAGLFTLFSWIIRAFT
                     WYANDLQDDPYLALIHFPVIVIFLAIGGFLTYLGVRGRRATRQ"
     gene            551..1690
                     /locus_tag="VFT03_00780"
     CDS             551..1690
                     /locus_tag="VFT03_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HEU4846704.1"
                     /translation="MAELKQRTLDEVKALSRDEAVEIMRQAGIVGAGGGGFPTYFKWK
                     NPQPRLIVNCTESEPGYWADKMLHREYFDEFLELFDVMKTVFGVEQIIMGVHFKDEEW
                     FSRYDENADRLYEVVHVPDKYSMGEEKTLIKNIFYEDKEKWVPRRVELPDGNKRPGLP
                     LDAGHIVNNSETLLNIYNAFFLGKPVTTKYLQVFGPDLPLKLYEAPLGASATELVSLA
                     GVDVEAEAGKLSIIDGGPYLNEMGIESLGEGDAFVRRTTNGFLVIPKGVASKEYADIK
                     TRCPEEGIVSLVGEVSGVDLPLGGRFLKPATPLISVGEEVEYGQKIGEPVDEGFSIGV
                     WASMAGKVSSIEDDVVAISGGAMPQEEARKEAEAARKEAEAEATR"
     gene            complement(1750..2622)
                     /locus_tag="VFT03_00785"
     CDS             complement(1750..2622)
                     /locus_tag="VFT03_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEU4846705.1"
                     /translation="MTEIGEKMRAFVFPGQGSEVTEPGAELLPRIRRVVGERIPDQVK
                     LFARAAQDADESRALQVRPDVVAGHSLGEYGAAYAAGCFDFETGLWLVAERDRFLAEA
                     ARETPGGMVAILRADPEEVERALDKTEGPVVANYNSPRQTVVSGLRDAMGDAVSRIRG
                     RKVPLNVSFAAHSPYVAAAGEKMRGVLDAVEFRKPEVPIVSAIDGSVLTSAGAVKEAL
                     KSQMVAPVRWVKVVDTLARMRVEELIELGGGGVLIRMLRDFELPSLKGITFDDLHSRL
                     SQQAQNFLPRSNGE"
     gene            complement(2822..3697)
                     /locus_tag="VFT03_00790"
     CDS             complement(2822..3697)
                     /locus_tag="VFT03_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU4846706.1"
                     /translation="MREAHLVLNPAADMGRAGGRRAYVSRFLEERGIRAVWHATLGPG
                     DGGRIAGGLPDGALAVAMGGDGTVHEVAAACVGRDLTLGVLPVGSGNDYVKALGIGTD
                     LGRALGVLAAGKVRVVDAGQVNGVRFINALGVGFDAEVAAGVARAPAYLGGTGRYLWS
                     VGQLLRGFRCHEATLRLDGGEVIEAETILVAVALGTTSGARFRLAPEARLDDGLFDVV
                     WSEEVSRAEVLRLIPAALGGTVLRHPKVHLARAREVEVELAKEVPAHVDGEILAPTRT
                     FKARVLPGALRVVAP"
     gene            4146..4595
                     /locus_tag="VFT03_00795"
     CDS             4146..4595
                     /locus_tag="VFT03_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846707.1"
                     /translation="MDLEGVARWVREAERTRDESYSLVEGERFGGIVREELQREVYRR
                     RRVMYDLQSVPRVATARPEMILVVYNGRSASYECRIFYKEPRPAGVVEQLSVEAGWRA
                     ILDLRTDPDPIVRLTSEKVEEFHVLRNRFSGEGAPAPGRRVFYANEL"
     gene            complement(4754..5299)
                     /gene="thpR"
                     /locus_tag="VFT03_00800"
     CDS             complement(4754..5299)
                     /gene="thpR"
                     /locus_tag="VFT03_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU4846708.1"
                     /translation="MRAFVAVFPPPEVQEALAGAARGLPIAGEVRLTPTANVHLTLKF
                     LGDVPEDDLGQVAEALEPLREEHEPFEAGISGFGAFPSPKKARVLWAGVGEGSDRLRA
                     LAEDVEKSLEPLGFEREGRIYVPHLTLGRARGRPVALETVERTSPIPAFPVRRVDLVQ
                     SAPGKDGVVYSTLAAYPLRTP"
     gene            complement(5308..6120)
                     /gene="larB"
                     /locus_tag="VFT03_00805"
     CDS             complement(5308..6120)
                     /gene="larB"
                     /locus_tag="VFT03_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEU4846709.1"
                     /translation="MPDLPDELDKILAAVAGGALSPSAAAGRLRNGEVRYLDEFAVLD
                     LGRQARKGVPEVVYAPGKTPEQVARICETLLGSTERVIVSGSSPEVTAELRRSLPGTP
                     LRSAGRAVVVGAGEPAPSGGRIGAICAGTSDVPVLGEAVAVAREMGAAVRSFHDVGVA
                     GIHRLTRPLEELRSFDPDCLIVAAGMEGALPSVVAGLVAVPVVGLPTSTGYGLGGDGT
                     AAILGMLQTCSPGLSVVNVDNGIGAGVTAALVANRAALGRASEPNGKEPGPG"
     gene            6195..7208
                     /locus_tag="VFT03_00810"
     CDS             6195..7208
                     /locus_tag="VFT03_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846710.1"
                     /translation="MTIMIIELVLSAMAIVVAAALFTNAVEILGDRLNLGQGAVGSVL
                     AAVGTALPETMIPAVAIVASIFTGSSAAGEVGVGAILGAPFLLATLAMFVVGVSALSF
                     RRRRESDDDVMIDKGTAKRDMRFFLILFALGAAAGIAPVPVYVKAALAVLLLVAYAFY
                     VWRTIKSGGESIEDPPGKLTLWRSGSEAPMGAVAAQLLGTVVVMGAGAHYFVSAVEHL
                     SEAAGLPPGLIALVLAPLATELPEKFNSVIWLRSNKDTLALGNITGAMVFQSSVPVAL
                     GLAFTSWSLDLLNALSAGLALLGGAYLYVLVSRKEPIEAEYMLVGGALYVVFLAVALV
                     TIL"
     gene            7312..8019
                     /locus_tag="VFT03_00815"
     CDS             7312..8019
                     /locus_tag="VFT03_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU4846711.1"
                     /translation="MFLDPIFWVALVKVLTVNLILSGDNAVVIAMAARSLHGSQRRQA
                     IIWGAAGAVALRLVFAAIIAFLLQIPLLQVAGGLLLIWIAWKLVLDDVEEGEDKIEAG
                     QSAFQAIRIIIIADAVMSLDNVIALVGAARLGGEVNWWLMGIGLGTTIPLVVFGAALL
                     TSLLDRFPILVYAGAALLIYIAVEMFFADEILHHYLEPFVNIEWIIGLSAAAIFTAIA
                     WLWKRRSESAEQEVSEE"
     gene            complement(8033..>8641)
                     /locus_tag="VFT03_00820"
     CDS             complement(8033..>8641)
                     /locus_tag="VFT03_00820"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU4846712.1"
                     /translation="AGTPLWPAGWVFMAIFFGFALVIVLWLGREDPGLLAERLSTPIQ
                     RGQPHWDKVFVVAVVLLFVFWLILMPLDAVRFGWSEVPGWLRILGAVGLVLSFYIIFL
                     TFRENAYLAPVVKLQLERGQSVVSTGPYRFVRHPMYASMFLFFPATALLLGSWWGLLL
                     CPVILGLLVWRTTLEDRLLQNELAGYDAYARNVRYRLIPGVW"
BASE COUNT         1509 a         2805 c         2819 g         1508 t
ORIGIN      
        1 aattctatgg ctcgatgtat ccagtttttc ggcacgcgtt gtcagcacgc tccggcttcg
       61 ccagcttgtc agcacgctcc ggctaacgcc tccgcttgtc agcatttcag ctattctctc
      121 tgtgctcaga gaagctgaca agccctcgaa gagggcgtgc tgagagcgag gccctaaaag
      181 ggcgtagcgt gctagttcac tgcctggtgg ccctgcggcc cctgactcca aggtatgtga
      241 ggaagccgcc gatggcgagg aagatcacga tgaccgggaa gtgtatgagg gcgaggtaag
      301 ggtcgtcctg caggtcgttc gcgtaccagg taaatgccct gattatccac gagaagagcg
      361 tgaaaagacc cgccgccaaa agcacgttcg atgtgcgcct gccgagcatc atgactgccg
      421 gagagtgtac cagaggcgag gccgctccaa ctagccacat tctggcgcaa aggttatact
      481 tcgcgtaaca gttggatatg gtttgcaaca gcaagtacct gatgtataga gcttacggag
      541 gcgagttgat ttggctgagt taaagcagcg cactctcgac gaggtaaagg ccttgagcag
      601 ggatgaggcg gtcgagatca tgcgccaggc cggcatcgta ggtgccggtg gcggtggctt
      661 cccgacgtac ttcaagtgga agaacccgca gccgcgtctg atcgtcaact gcaccgaatc
      721 ggagcctggc tactgggctg acaagatgct ccaccgcgag tacttcgacg agttcctcga
      781 gctcttcgac gtgatgaaga cggtcttcgg ggtagagcag atcatcatgg gcgtccactt
      841 caaggacgag gagtggttct cgcgctacga cgagaacgcc gaccggctct acgaggtcgt
      901 ccacgtcccg gacaagtact cgatgggcga ggagaagacg ctcatcaaga acatcttcta
      961 cgaggacaag gagaagtggg tgccgcgcag ggtcgagctt cccgacggca acaaacgccc
     1021 cggcctcccg ctagacgcag ggcacatcgt caacaactcc gagacactgc tgaacatcta
     1081 caacgcgttc tttttgggca agccggtgac caccaagtac ctccaggtct tcggccccga
     1141 cctcccgctc aagctctacg aggcgcccct gggggcgtcg gccaccgagc tggtctctct
     1201 ggccggcgtg gatgtcgagg ccgaggcggg caagctctcc atcatcgacg gcggcccgta
     1261 cctgaacgag atgggcatcg agtccctggg cgagggcgac gccttcgtgc gccgcaccac
     1321 caacggcttc ctcgtcatcc cgaaaggcgt ggcgagcaag gagtacgccg acatcaagac
     1381 caggtgcccc gaggagggga tcgtctcgct cgtcggcgag gtctccgggg tcgacctccc
     1441 gctcggcggg aggttcctca agccggccac gccactcatc tcagtgggcg aagaggtcga
     1501 gtacgggcag aagatcggtg agcccgtgga cgagggattc tccatcggcg tatgggcgag
     1561 catggccggc aaggtttcct cgatcgagga cgacgtcgtg gccatctccg gcggcgccat
     1621 gccccaggag gaggccagga aagaggccga ggccgccagg aaagaggccg aagccgaagc
     1681 gacgaggtag gtttcaggct tcaggttggg ccggggcgcg aagccccggc cttatcgttt
     1741 gcctgaagcc tattcgccgt tggagcgagg gaggaagttc tgggcttgct gggagaggcg
     1801 gctgtgcagg tcgtcgaagg tgatgccctt cagggagggg agctcgaagt cccggagcat
     1861 ccggatcagg acgccgccgc cgccgagctc gatcaactcc tcgacccgca tcctggcgag
     1921 ggtatcgacg accttgaccc agcgaacggg cgcgaccatc tggctcttta acgcctcctt
     1981 gacggctccg gcgcttgtga gcaccgaccc gtctatggcg ctcacgatcg ggacctccgg
     2041 cttgcggaac tcgacggcgt cgaggacccc gcgcatcttc tcgccggcag ccgccacgta
     2101 aggggagtgt gccgcgaagg agacgttgag gggtaccttg cggccccgga tcctggagac
     2161 cgcgtcgccc atggcgtcgc gcaggcctga gacgacggtc tggcgcgggg agttgtagtt
     2221 tgcgaccacc gggccctccg tcttgtcgag cgctctctcg acctcctcgg ggtcggccct
     2281 gaggatcgca accatcccgc ccggcgtttc tcgtgcggcc tccgcgagaa agcggtccct
     2341 ctccgccacg agccagaggc cagtctcgaa gtcgaagcac ccggcggcgt aggcggcgcc
     2401 gtactccccg agcgagtgcc cggctaccac gtcggggcgt acctggagag cgcggctctc
     2461 gtcggcgtcc tgggcggcgc gggcgaagag cttgacctgg tccggtatgc gctcgccgac
     2521 gacacgccgg atcctgggca gcaactccgc ccccggctcc gtcacctcgc tgccttgtcc
     2581 cggaaacacg aacgctctca tcttctcacc gatctccgtc acaccctacc tatacgctct
     2641 agaccgcccc gtgttgcaag gacgctagag aataggcgtt ttaccacaga ttcgcgacgt
     2701 ttaatagggt ctcgacggga acatcgagta ccgcgaacgt tcggaaggct tctcagtccg
     2761 cctgggcgtc ccgacgcatc cggtttctgc tcctggacgg tacgccatcg ttgcttgcag
     2821 cctatggagc cacgacacgc aacgctccgg gcaacacccg tgccttgaaa gttcgggtag
     2881 gcgcaagtat ctcaccgtct acgtgagccg ggacctcctt cgccaactcc acctcgacct
     2941 cccgcgcacg cgccaggtgt accttggggt gcctgaggac cgtgcccccc agcgccgccg
     3001 gtatgagccg caggacctcc gcccggctga cctcctcgga ccagacgacg tcgaagagcc
     3061 cgtcgtccag cctggcctcc ggcgcgagcc ggaatctggc cccggaggtg gtcccgagcg
     3121 ccaccgcgac gagtatggtc tcagcctcga tgacctcccc gccatccagc ctgagcgtag
     3181 cctcgtggca cctgaagcct cggagcagct gccccacgga ccacaggtac ctgcccgtcc
     3241 ccccgaggta agccggcgcc cgcgctacgc cagccgctac ctccgcgtcg aagccgacgc
     3301 cgagcgcgtt gatgaaccgg accccgttga cctgccccgc gtccaccacc cggaccttcc
     3361 ccgccgcgag gaccccgagc gcccgcccca ggtccgtgcc gatccccagc gccttgacgt
     3421 agtcgttgcc gctgcctaca ggcagcaccc ccaacgtcag atccctcccc acgcaagccg
     3481 ccgcgacttc gtgtaccgtc ccgtccccgc ccatcgccac ggcgagcgcc ccgtcaggca
     3541 accccccggc gatgcgaccc ccgtcaccgg gtcccagcgt cgcgtgccag accgccctga
     3601 tccctcgctc ctccaggaaa cgcgagacgt acgcgcgtcg gccgccagct cgacccatgt
     3661 cggccgccgg gttcagcacg aggtgtgctt ctctcatccg caaggtaatg tggaaaatac
     3721 cagagccgcg cccccaggat ctcgccggac aaccggtata aggctcagct cgaaggtgaa
     3781 gcttcgcgcc gggaggacgc gcccccgttc gggtgcgcgg ggcaaactct ctccacgccc
     3841 aggaccgggt tccccgcgtc gtctgcgagt tgggggcgat ggaaccgccg ttatctcgga
     3901 gaacggcttg tagatccgtc gagaggacgc tcgagggctg ttcgctgtga gtatcgacct
     3961 ttcgtccata ttaagttata gtacaggtac acaatatcct cgggcttcgt attagagtcg
     4021 cgccgggtta ggaaaggcgt acaatactgg tctacatggc gagcgcggcg gaaaattgcc
     4081 tgcatctgtg gggcttgcgg ccttctgcct ggtccgagta ccggtgcccg gcttgcgggg
     4141 agaaaatgga tctcgagggc gttgcgcggt gggttcggga ggccgagaga actcgcgacg
     4201 agtcttattc tttagttgag ggtgaaaggt tcggcggcat cgtgcgcgaa gagttgcaga
     4261 gggaagtcta ccgcaggcgg agggttatgt acgacttgca gagcgtaccc cgcgtggcga
     4321 cggcgaggcc ggagatgatc ctggtcgtct ataacggccg gagcgcatct tacgagtgcc
     4381 ggatcttcta caaggaaccg cggcctgccg gggtagtaga gcagctcagc gtggaggcgg
     4441 ggtggcgggc catcctggac ttgcgcaccg acccggatcc catcgtgcgg ctcacgtcgg
     4501 agaaggtgga ggagttccac gtcctgcgta acaggttctc gggcgagggc gcgccggcgc
     4561 cgggccggcg agtgttctac gcgaacgagc tctagggcac gctgctggca ttcgcctggt
     4621 acggcggcta gaataggccg gccgttgctg ttctccttcc agggatgagg tgtgatgagc
     4681 gggaacaact tcatagcgat agtcgttatg atcctgatac tgctcttcat cctcatgtcc
     4741 gtcggcccgc tgatcacggc gttctgagag ggtaagccgc cagggtagag tagacgacgc
     4801 cgtctttgcc gggcgcgctc tgcaccagat ccacccgtcg aaccgggaag gcgggtatgg
     4861 gcgaggtcct ctccaccgtc tccagagcca ccgggcgtcc gcgcgcccgg ccgagcgtaa
     4921 ggtggggaac gtagatcctg ccttcgcgct cgaagcctag aggctccagg gacttttcca
     4981 cgtcttcggc cagggcgcgc agcctatccg aaccttcgcc gacgccggcc cacaagacgc
     5041 gagccttctt cggggagggg aaagctccga agccggagat cccggcctcg aacggctcgt
     5101 gttcttcgcg gagtggctcg agcgcctccg ccacctgacc caggtcgtcc tcggggacgt
     5161 caccaaggaa cttcagcgtc aggtgaacgt tcgcggtggg agtgaggcgg acttcgcccg
     5221 ctatggggag cccgcgcgcc gcgccggcca gcgcctcctg gacttcgggc ggtgggaaga
     5281 cggcgacgaa ggcccgcatg ctcgggccta gccgggaccc ggctctttgc cgttgggttc
     5341 gctcgcgcgg ccgagggcgg ctctgttggc gacgagggcc gcggtaacgc cggccccgat
     5401 gccgttgtcc acgttcacta cggagaggcc gggcgagcag gtctggagca tgcccaggat
     5461 ggcggctgtg ccgtcgccgc cgaggccgta gccggtcgag gtgggcaggc cgacgaccgg
     5521 gacggccacg agcccagcta ccacgctcgg cagtgccccc tccataccgg ccgccactat
     5581 caggcagtcg gggtcgaagc tacggagctc ctccagaggt cgggtgaggc ggtgtatacc
     5641 ggccaccccg acgtcgtgga agctcctgac ggcagcgccc atctccctgg ccaccgcgac
     5701 cgcctccccg agaaccggca cgtccgaggt gcccgcgcat atggcgccga tgcggccgcc
     5761 cgagggcgcg ggttctccgg ccccgacgac gacggcgcgg ccggcgctcc tcaagggcgt
     5821 tcccgggagg gagcgccgga gctccgccgt cacttcgggt gatgagccgc tgacgatcac
     5881 gcgctcggtc gagcccagga gggtctcgca gatccgggcc acctgctccg gcgtcttgcc
     5941 cggggcgtag acgacttcgg ggacgccctt gcgcgcctgg cggccgaggt ccaggaccgc
     6001 gaactcgtca aggtagcgta cctctccatt tctcaggcgc cccgctgctg ccgaaggcga
     6061 gagggcgccg ccggccaccg cagccagtat cttgtccagc tcatcgggta agtcaggcat
     6121 gagcctaagt gtaaccgctg ccgccgccgg gtagaatcct ccggtccggc acggtcgctg
     6181 aagaaagggc gccgatgacg atcatgatca tagaactggt gctctccgcg atggccatcg
     6241 tggtggcggc cgcgctgttc accaacgcgg tcgagatcct cggcgacagg ctgaatctgg
     6301 gtcagggggc ggtggggagc gtgctcgccg ccgtcggaac cgcgctgccc gagaccatga
     6361 ttcccgcggt cgccatcgtc gcgtctatct tcaccggcag cagcgcggcg ggagaggtcg
     6421 gcgtcggggc tatcctcggg gcgccgttcc tccttgccac gctcgcgatg ttcgtcgtcg
     6481 gggtttctgc tctgagcttc cgccgccgcc gggagagcga cgacgacgtg atgatagaca
     6541 aagggaccgc gaagcgcgac atgcgatttt tcctcatcct cttcgctctg ggcgctgcgg
     6601 ccggtatcgc gccggtgccc gtctacgtga aggcggcact ggcggtcctg ctccttgttg
     6661 cttacgcgtt ctacgtgtgg cgcaccataa agtctggcgg agagagcatc gaggacccgc
     6721 cagggaagct gactctgtgg cgctcggggt ccgaggctcc catgggggcc gtggcggcgc
     6781 agctcctcgg caccgtggtc gtgatgggag cgggggccca ctacttcgtc agcgccgtgg
     6841 agcacctctc cgaggcagcg gggctgccgc cgggtttgat cgcgctcgtg ctcgcgcctc
     6901 tggccacgga gttgcccgag aagttcaact ccgtcatctg gcttcggagc aacaaggaca
     6961 ctcttgccct gggcaacata acgggggcta tggtcttcca gagctcggtg ccggtcgccc
     7021 tcggcctcgc gttcacgagc tggagcttag atctgctcaa cgccctctcc gcgggcctcg
     7081 cgcttttggg aggcgcgtac ctctacgtgc tggtcagtag gaaagagccg atagaggccg
     7141 agtacatgct cgtcggtggg gcgctctacg tcgtgttcct cgccgtagcg ctcgtcacca
     7201 tcctctgatg ccggttcgat ggaaaggtcg gattgttggt aagataagcc ggggttggta
     7261 caagcaggag cggtcccggc gtctggcggg aaggttcagg aggagcacac gttgttttta
     7321 gatccgattt tctgggtcgc gcttgtcaaa gtacttaccg tcaacctcat cctctcgggc
     7381 gacaacgccg tcgtcatcgc catggcggcc cgcagcctcc acggctccca gcgccggcag
     7441 gcgatcatct ggggcgccgc cggggccgtc gcgctgcggc tcgtcttcgc ggccatcata
     7501 gccttcttgc tccagattcc cttgttgcag gtcgcaggag ggcttttgtt gatctggatc
     7561 gcctggaagc tcgtcctcga cgatgtcgag gagggagagg acaaaatcga ggccggccag
     7621 agcgcgtttc aggcgatcag gatcatcatc atcgccgacg ccgtcatgtc gctcgataac
     7681 gtgatagccc tcgtcggcgc cgccaggctc ggtggagagg tcaactggtg gctaatgggg
     7741 atcggcctcg ggacgacgat accactggtc gtcttcgggg cagcgctgct cacctccctg
     7801 cttgaccggt tccccatact cgtctacgcc ggcgccgcgc tcctcatcta catagccgtg
     7861 gagatgttct tcgccgacga gatactccac cactacctcg agccgttcgt gaacatcgag
     7921 tggatcatag gcttgagcgc cgcggcgatc tttaccgcca tcgcctggct gtggaagagg
     7981 cgctccgaga gcgccgaaca ggaggtcagc gaggagtagc ggcgcttggt agtcaccaga
     8041 cgcctggtat aagccggtac ctcacattac gtgcgtacgc gtcgtatccg gcaagctcgt
     8101 tctgtagcag ccggtcctcg agggtggtcc tccagacgag tagccccaga atcacggggc
     8161 acagcaggag cccccaccac gagcccagca ggagcgcggt tgccggaaag aacaagaaca
     8221 tgctcgcata catgggatgc ctcacgaagc ggtacggccc ggtgctcacg acgctctgac
     8281 ccctctccag ttgcagtttc accacagggg cgaggtacgc gttctcccgg aaggtcagga
     8341 aaatgatgta gaacgagagc acgagtccca ctgctcccag gatccgtaac cagccgggca
     8401 cctccgacca gccgaacctc acagcgtcca ggggcatcaa gatcagccag aagacaaaga
     8461 gcagcactac cgcaaccacg aacaccttgt cccagtgagg ttgccccctc tgtatcgggg
     8521 tggagagcct ctctgccagg agaccggggt cttcgcggcc cagccagagt acgatgacca
     8581 gggcaaatcc gaagaagatc gccatgaaca cccatccggc gggccagagc ggcgtccccg
     8641 c
//
LOCUS       DASTUV010000040         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1390712, whole genome shotgun sequence.
ACCESSION   DASTUV010000040 DASTUV010000000
VERSION     DASTUV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390712"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFT03_00825"
     CDS             <1..275
                     /locus_tag="VFT03_00825"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846713.1"
                     /translation="RGTTHIDLALVEDSLSGARDTMEDVITGKVSIADLARDTGEKIE
                     AENGEAPHPPMTVTMVCTVCGEELEGVAPPEECPVCGAPGEDFESK"
     gene            332..601
                     /locus_tag="VFT03_00830"
     CDS             332..601
                     /locus_tag="VFT03_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4846714.1"
                     /translation="MARTHKVTFKKSGVTVDVAEDEYILEEAEDAGLRLPYDCRSGTC
                     TTCMQKCLEGEIDQDLAFAISDEELEKGYRLICIGSPLSDVVLDA"
     gene            688..1365
                     /locus_tag="VFT03_00835"
     CDS             688..1365
                     /locus_tag="VFT03_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4846715.1"
                     /translation="MRPGTRVLLVEDDRSISGFVEPELERLGFRVRCAYDGPSGLKEA
                     RRFRPELVVLDIMLPRLDGVGVLKELRRGGSKVPVVMLTARDTTLDKVHSLDLGADDY
                     VTKPFEMGELLARIRALLRRAEGEEILRVADLEVNTATREVRRGERDIDLTAREYELL
                     EFMARNPRRVLSREFLLSRVWDQEFGVTTNVVEVYIAYLRKKVDAPGEQKLIHTVRGA
                     GYALKGG"
     gene            1372..>2112
                     /locus_tag="VFT03_00840"
     CDS             1372..>2112
                     /locus_tag="VFT03_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU4846716.1"
                     /translation="MPIRWRLTLFIALVIGAILIVLGFALYFLSRSALLTNVENTTRS
                     RAISAANTIESGKLLSGDDVEQLTLDGVSVIVRDGSGEAIQTANFPASGEARDPVWRE
                     AVRSGKAASGMAVLAGDDPSYIYAVPVDPPGGPARVVEAASPYEPVQDTLEVLGTVLV
                     AGIGAALLLSIGGAYFLAGAALRPIDAVTSAAKEMGEGDLSKRLPVANPGDEVGRLAT
                     TINGFLSRLEAAFARREEALERQRRFAAD"
BASE COUNT          386 a          668 c          721 g          337 t
ORIGIN      
        1 agcgcggcac gacgcacata gacctcgcgc tcgtcgagga ctccttgagc ggggcgcgcg
       61 acactatgga ggacgtcata accggcaagg tctccatcgc cgacctcgcc cgcgacacgg
      121 gcgagaagat agaggccgag aacggtgagg ccccacaccc gccgatgacc gtcaccatgg
      181 tctgcaccgt ctgcggcgag gagctggagg gtgttgcgcc ccccgaagag tgccccgtct
      241 gcggcgcgcc cggcgaagat ttcgaatcaa aataggtatc aggcttcagg catcaacatt
      301 aggcttgtgc ctgaagcctg agatctgacc agtggctagg acgcacaagg taacgttcaa
      361 gaagagcggc gtcaccgtgg atgtggccga ggacgagtac atcctcgagg aggccgagga
      421 cgcggggctg cgccttccgt acgactgccg cagcggcacg tgtaccacgt gtatgcagaa
      481 gtgcctggaa ggcgagatcg accaggacct cgccttcgcc atctccgacg aggagctcga
      541 gaagggctac cgcctaatct gcatcggcag ccctctctcc gacgtcgtgc tcgacgcctg
      601 accggcggtt ctagttctaa gccctctctc aggaaagtcg cttacgatct tcgtgactta
      661 gaatgtcggc acgaggttgg tgaggagatg agaccgggaa cgcgcgtgct gctggtggag
      721 gacgaccgct cgatctccgg cttcgtcgag ccagagctcg agcgcctcgg tttccgcgtg
      781 cggtgcgcct acgacggtcc ctccggcctt aaggaggccc ggcggttcag gccggagctc
      841 gtcgtcctgg acatcatgct gcccaggctg gacggggtag gggtgttgaa ggagctaagg
      901 cgcgggggca gcaaggtgcc cgtggtcatg ctcaccgccc gcgacacgac gctggacaag
      961 gtccacagcc tcgacctcgg agccgacgac tacgtgacca agccgttcga gatgggcgag
     1021 ctgttggccc ggatacgggc gctgctcagg cgcgctgaag gtgaggagat cctgcgtgtc
     1081 gccgacctgg aggtgaacac cgcaacccgc gaggtgcggc gcggggagcg ggatatagac
     1141 ctcacagccc gcgagtacga gctgctggag ttcatggccc gcaaccccag gcgggtcctc
     1201 tcgcgggagt tcctgctctc cagggtctgg gatcaggagt tcggcgtgac aaccaacgtc
     1261 gtcgaggtct acatcgccta cctcaggaag aaggtagacg cgccgggtga gcagaagctc
     1321 atccacaccg tcaggggcgc cgggtacgcc ctgaagggag gatagagcgc cttgccgatc
     1381 cggtggcgcc tcacgctctt catcgcgctg gtgatcggcg cgatcctcat cgtccttggc
     1441 ttcgcgctgt atttcctgag caggagcgcg ctgctcacca acgtcgagaa cacaacgcgg
     1501 agccgtgcga tctcggccgc taataccatc gagtccggaa agctcttgag cggcgacgac
     1561 gtcgaacagc tcaccctcga tggggtatcc gtgatcgtac gcgacggaag cggcgaggcg
     1621 atccagacgg cgaacttccc ggcgagtggc gaagccagag accccgtgtg gcgggaagcg
     1681 gtccgctcgg gaaaagctgc gtcggggatg gccgtgctgg cgggagacga cccctcctac
     1741 atctacgccg taccggttga tccgccgggt ggaccggcgc gggtggtcga ggccgccagc
     1801 ccctacgagc cagtccagga caccctcgag gtcctgggga ccgtgctcgt ggccggcatc
     1861 ggcgccgccc ttctcctgtc catcggcggc gcctacttcc tggcaggagc agccctgagg
     1921 cccatagacg ccgtgacgag cgccgcaaag gagatgggcg agggtgacct ctcgaagcgg
     1981 ctgcccgtgg ccaaccccgg ggacgaggtc gggcgtctcg ccaccacgat aaacggtttc
     2041 ctctcgcgcc tggaggccgc cttcgcccgc agggaagaag ccctggagcg ccagcggcgt
     2101 ttcgcggccg ac
//
LOCUS       DASTUV010000041         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_173465, whole genome shotgun sequence.
ACCESSION   DASTUV010000041 DASTUV010000000
VERSION     DASTUV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173465"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFT03_00845"
     CDS             complement(<1..342)
                     /locus_tag="VFT03_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4846717.1"
                     /translation="MLRADLVSGILPVREDGRMLLLQRPTGTWDPPAGRLSTGESFED
                     GAVRELYEETGLLVAPRRILATWVGEAPSGRLLAAVTYIARTRDVEVSLSHEHLGYRW
                     VTLDEWLELPSW"
     gene            complement(381..1151)
                     /locus_tag="VFT03_00850"
     CDS             complement(381..1151)
                     /locus_tag="VFT03_00850"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEU4846718.1"
                     /translation="MQALQPKMVLSRTEHRPYPMPAGPWVLFMSWHDLLFMHWPVHEE
                     ALRSLIPPVLHLDTFGGSAWLSVTPFRMTGVRPRFLPSVPPLSNFPELNVRTYVTAGG
                     KPGIWFFSLDAGNPVAVRLARATFHLPYLDAQMSCAVVGDEVHYRSVRTQAGTPGAEL
                     AARYRPVGEPSESRPGTLENFLTERYCLYAADGRGNVYRGDIHHHPWPLQPAEAEVEK
                     LTMTEQIGVALPEPGPILHFSERLDVLAWSPRRITLPG"
     gene            complement(1198..1836)
                     /locus_tag="VFT03_00855"
     CDS             complement(1198..1836)
                     /locus_tag="VFT03_00855"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU4846719.1"
                     /translation="MRAAIFLNGYPDPPDLLRSVAGRADLVVAADGGARYALDAGVVP
                     DLVVGDMDSLGEAGAREVAERGALLERHPVRKDKMDAHLAVIAAGERGATDVDLVCAT
                     GGNFSSVFAVPHILLTAERMGLRATMRAGWGQVFVLETGTRTVSGDPGDSVSIFPLTG
                     PAAGVTLEGFSYPLEDARLEIGDTLGFHNELVGGAGRVSVEEGSLLVIHETL"
     gene            complement(1838..2398)
                     /gene="thiT"
                     /locus_tag="VFT03_00860"
     CDS             complement(1838..2398)
                     /gene="thiT"
                     /locus_tag="VFT03_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEU4846720.1"
                     /translation="MNAFKDTRVLAEAALAIALAFVLGLIKVFHMPFGGSISLEMVPL
                     ILLALRQGPAVGIVAGAAYGLLNFAIDPIALHPVQVIFDYPLAFGALGLAGFFKPTVR
                     GAILGTIVAVLARFLCHFVSGVVFFASYAPEGWNPYLYSAAYNAGYLVPSLAIALVVV
                     VVLLKALEGAQPSARQARYRRRVKTT"
     regulatory      complement(2431..2532)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2546..>2850)
                     /locus_tag="VFT03_00865"
     CDS             complement(2546..>2850)
                     /locus_tag="VFT03_00865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4846721.1"
                     /translation="EDPGEAARRELFEETGFRAEGLEHLVSVHTSTGRTTEVCHLFRC
                     RAVRDPKGPRPEATEFIEVVEMPLGEALGMVSEGGITDAATVLGLLWSGHLSSDRG"
BASE COUNT          499 a          966 c          944 g          441 t
ORIGIN      
        1 ccagctcggc agctcgagcc actcgtcaag ggtgacccac ctgtagccga ggtgctcgtg
       61 cgagaggctc acctccacgt cgcgcgtgcg ggcgatgtag gttactgcag ccaggagacg
      121 gccgctcggt gcttccccga cccaggtggc gaggatgcgc cggggcgcga cgaggaggcc
      181 ggtctcctcg tagagctcgc gcacggcccc gtcctcgaag ctctcgccgg tagacagacg
      241 cccggccggc gggtcccagg tgcctgtcgg gcgctggagc aggagcatgc ggccgtcctc
      301 ccggacgggc aggatgccag agacgaggtc cgcccttaac atatgccctc ataatccacg
      361 cgccgagtat aacttgagcg tcatccaggt aaggtgatcc gccgcggcga ccacgcgagc
      421 acgtcgaggc gttcggagaa gtgcaggatc ggccccggct ccggtagcgc gacgccgatc
      481 tgctccgtca tcgtgagctt ctcgacctcg gcctcggcgg gttggagggg ccagggatgg
      541 tggtggatgt cgccgcgata gacattgccc ctgccgtccg cggcgtagag gcagtagcgc
      601 tcggtcagga agttttcgag cgtgccggga cggctctcgg acggctcacc cacgggtcgg
      661 tagcgggccg ctagctccgc ccccggcgtt cccgcttggg tgcgcacgct cctgtagtga
      721 acctcgtcgc ccacgacagc gcaggacatc tgcgcgtcca agtacggcag gtggaaggtg
      781 gcgcgggcga ggcggacggc caccggattg ccagcgtcca gcgagaagaa ccagatgcca
      841 ggcttgcccc cggccgtgac gtaggtgcgg acgtttagct ccgggaagtt ggatagggga
      901 ggaacgctcg gcaggaagcg cggccgcaca ccggtcatgc ggaaaggcgt gacgctcagc
      961 cacgcgctgc cgccgaaggt gtcgagatgc aggacgggcg gtatcaggga tcggagcgcc
     1021 tcctcatgga cgggccagtg cataaagagc aggtcgtgcc agctcataaa gagaacccac
     1081 gggccagccg gcatgggata gggccggtgc tctgtccggc tcaggaccat cttgggctgc
     1141 aacgcctgca tgatcctcgc aatcctatga caccggcgcc cggccgtcga gcgcttttca
     1201 gagggtctcg tggataacca gcagagagcc ctcctccacg ctgacccggc ccgctccacc
     1261 gacaagctcg ttgtgaaacc ccagagtgtc tcctatctcc agcctggcat cctccagcgg
     1321 atagctgaat ccctcgaggg tcaccccggc agccggaccg gtgaggggga agatcgagac
     1381 gctatctcct ggatctccgg agacggtcct ggttcctgtc tccaggacga acacctgccc
     1441 ccaaccggct ctcatggtgg ccctcaagcc catccgctcg gcggtgagca ggatgtgcgg
     1501 tacggcgaac actgagctga agttgcctcc ggtggcacag acgagatcca cgtccgtagc
     1561 ccccctttcc ccggcggcaa taaccgcaag gtgggcgtcc atcttgtcct tcctgaccgg
     1621 gtggcgctcg agcaacgcgc cacgctcggc gacctcccgc gcccccgctt cgcctaaaga
     1681 gtccatgtcc ccgacgacga ggtccgggac caccccggcg tccagcgcat agcgagcccc
     1741 gccgtccgcg gctaccacca gatccgcccg tccggctacg gaccggagca gatccggcgg
     1801 gtccgggtag ccgttcagga agatggcggc ccgcaagcta ggtggtcttc acgcgccgcc
     1861 ggtagcgcgc ctgacgcgcc gagggctgcg ctccttcgag cgccttgagc agcaccacca
     1921 cgacgaccag ggcgatggca aggctcggca ccaggtaccc ggcgttgtac gccgccgagt
     1981 agaggtaggg gttccatcct tccggcgcgt aggaggcgaa gaagacgacc cccgatacga
     2041 agtggcacag aaagcgggca agcaccgcta ctatggtgcc gagaatggcg ccccgaacgg
     2101 tgggcttgaa gaacccggcg agccccagcg ctccgaaggc cagcgggtag tcgaagatca
     2161 cctgcacggg gtggagagct atcgggtcga tggcgaagtt caggagaccg tacgccgctc
     2221 cggcgacgat cccgacggcc ggtccctggc gcagggccag caatatcaag ggcaccatct
     2281 caagcgagat cgagccgccg aacggcatgt ggaagacctt gatcaagccc agaacgaacg
     2341 ccagggctat ggcgagcgcc gcctccgcga gcaccctggt gtctttgaac gcgttcaccg
     2401 cgcaccctcc ttcgtctctg cggggatgcg cgaaagagtc tcgaatctcc ctgcgccggt
     2461 attacccgga tcaggttcag cgggtcggcg cctctgcgcc ctctcagcct cccggctccc
     2521 cggtctattg caccctaaat gtatcttatc cgcggtcgga ggacaagtga cccgaccaca
     2581 ggaggcccag caccgtggcc gcgtcggtga tgccgccctc ggagaccatc cccagagcct
     2641 ccccgagcgg catctctact acctctatga actctgtcgc ttccggtcgg ggacctttgg
     2701 ggtcgcgcac ggcccggcac cggaagagat ggcagacctc tgtggtacgc ccggtgctgg
     2761 tgtgtaccga gacgaggtgc tcgagcccct cggccctgaa cccggtctcc tcgaagagct
     2821 cgcgccgggc ggcctcaccg gggtcctcgc
//
LOCUS       DASTUV010000042         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_567044, whole genome shotgun sequence.
ACCESSION   DASTUV010000042 DASTUV010000000
VERSION     DASTUV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567044"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFT03_00870"
     CDS             <1..277
                     /locus_tag="VFT03_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846722.1"
                     /translation="SHREEAEMFLRLGSSGYDWRASGSGPRELVLILYGSCADVRDVA
                     LDPLPEMLADRSLGLVSLCRERFLQRLTWPEGDYSSAVYRSRGTRRA"
     gene            304..990
                     /locus_tag="VFT03_00875"
     CDS             304..990
                     /locus_tag="VFT03_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEU4846723.1"
                     /translation="MQQLPLSGYILVMIAGALVSGAAALWVRSTYSKYSKQPSRSGLP
                     GAQVARAILDRNNLSKVRVEPVAGTLTDHYDPRRKVIRLSESNYRSNSIAAVSVAAHE
                     CGHAIQDASGYLPMKLRSGLFPLVAFSSQIWLPLFFIGVFVGAPLFIQLAAILFGAVL
                     AFHLVTLPVEINASTRAYELLTRYGLLSREEANGTRRVLSAAAFTYIAAALTAFLTLL
                     YLLFLSQSNE"
     gene            1027..1629
                     /locus_tag="VFT03_00880"
     CDS             1027..1629
                     /locus_tag="VFT03_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEU4846724.1"
                     /translation="MTTNNASSPAGRGVLPVLARNWWALALRGLAAILFGVLAFAWPG
                     ITLFVLVLFFGAYMLVDGIFAIVAAVRAAGEEDRWWLLLIEGILGVLAGLVAFFWPGL
                     TALALLYFIAAWAIVTGIMEIVAAIRLRQEIEGEWALGLSGLLSVIFGILLVVLPAPA
                     GLLSLVWLVGAYAVATGVLLLILAFRVRNEASNEARARPS"
     gene            1775..2323
                     /locus_tag="VFT03_00885"
     CDS             1775..2323
                     /locus_tag="VFT03_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4846725.1"
                     /translation="MMSRYRGRGFYDPLSEMNRLFAQVFGLAPQRTGDGGGQGDGGEE
                     GRAMTAWTPAIDVLQRDGDLVVRAELPGVEPDNVDITVQNNILTISGRISEEREEERE
                     GYLIRERRAGFFRRSLQLPQGVDESNITARFDNGVLEVVIEGAAQAQEPRHVEVEGQQ
                     ESGNGGQQSGRQQQDAQSGSSS"
BASE COUNT          460 a          813 c          935 g          455 t
ORIGIN      
        1 cagccaccgg gaggaggccg agatgttcct tcggctcggg agctctggtt acgactggcg
       61 cgctagcggg agtggtcccc gggaacttgt gttgattctc tacggttcct gtgcggatgt
      121 gagggacgtc gccctcgacc ctttgccgga gatgctggcg gataggagct tggggcttgt
      181 ctcgctgtgc cgggagcgtt tcctgcagcg cctcacatgg cccgaggggg actactctag
      241 cgctgtgtat cgaagcagag ggacccgccg cgcatgacac gggccagggg ataggtttta
      301 tctgtgcagc agctaccgtt gtcaggctac atactcgtga tgatcgcggg cgccctcgtc
      361 tccggggccg cggccctgtg ggtgcgcagc acttactcga agtactccaa gcagccgagc
      421 aggagcgggc tccccggcgc gcaggtcgcg cgcgcgatcc tggaccgcaa caacctctcg
      481 aaagtgcggg tcgagccggt cgccggcacg ctgacggacc actacgaccc caggcgcaag
      541 gtgatcaggc tctcggagag caactaccgg agcaactcga tagcggcggt gagcgtcgcg
      601 gcccacgagt gcgggcacgc catccaggac gcgagcgggt acctgccgat gaagctcagg
      661 tcggggctct tccccctggt ggccttcagc agccagatct ggctgcccct cttcttcatc
      721 ggcgtgttcg tgggggcgcc cctctttatc cagctggcgg ccatcctttt cggggcggtt
      781 ctggctttcc acctggtgac gcttccggtg gagatcaacg cttccacccg cgcctacgaa
      841 ctcctgaccc gctacggcct gctctcccgg gaggaggcga acggtaccag gcgggtactg
      901 tccgcggccg ccttcacgta catcgccgcg gcgctgaccg cgttcctgac gctcctgtac
      961 ctgctcttct tgagccagag taatgagtag tggagcacga aagcaacaga gaaaggaata
     1021 gaagagatga cgacaaacaa cgctagctct ccggcgggac ggggagtgct gcccgtcctc
     1081 gcgaggaact ggtgggcact ggccttgcga gggctcgccg ccatactctt cggcgtgctc
     1141 gccttcgcct ggcccggcat aaccctcttc gtcctcgtcc tcttcttcgg cgcctacatg
     1201 ctggtcgacg gcatcttcgc catcgtcgcg gcggtaaggg cggcgggcga ggaggacagg
     1261 tggtggctgc tcctgatcga ggggatcctg ggcgtcctgg ccggcctggt cgcgttcttc
     1321 tggcccggtc tcacggcgct cgcgctgctg tacttcatag cggcctgggc gatagttacc
     1381 gggatcatgg agattgtggc ggcgatcagg ctgcggcagg agatcgaggg cgagtgggcg
     1441 ctcggactta gcgggctgct ctccgtgatc ttcgggattc tactggtggt gctacccgcg
     1501 ccggcggggc tgctctctct ggtgtggctc gttggggcgt acgcggtcgc cacgggcgtg
     1561 ctgctcctga tcctggcttt ccgggtgcgc aacgaggcca gcaacgaggc cagggccagg
     1621 ccgagctaac cctggagaca cgcggtttct gcgatctagc acatgaggag gtgcgctacg
     1681 ggctgaacga aaaagagcaa cggtcgagac gtctcgctcc ggcggagaac gacggggcga
     1741 gcacaaccca cagagaacag gaggcgcaag cgagatgatg agccggtaca ggggtcgcgg
     1801 cttctacgac ccactgagcg agatgaaccg cctcttcgcc caggtcttcg gcctcgcccc
     1861 ccagaggacc ggggacggcg gaggacaggg cgacggcgga gaggaggggc gggcgatgac
     1921 cgcctggacc cccgccatag acgtcctcca gcgcgacgga gacctggtgg tcagggccga
     1981 gctcccgggc gtggagcccg acaatgtgga catcacagtc cagaacaaca tcctgaccat
     2041 ctcgggtagg atctccgagg agagggagga ggagcgcgag ggctacctga tcagggagcg
     2101 ccgcgcgggg ttcttccgca ggagcctgca gctgccccag ggggtggacg agagcaacat
     2161 caccgcccgc ttcgacaacg gcgtcctcga ggtcgtcatc gagggggcgg cccaggcgca
     2221 ggagcccagg cacgtagagg tcgagggcca gcaggagagc ggcaacggcg gtcagcagag
     2281 cggccgccag cagcaggacg ctcagagcgg ctccagcagc taggaggcgg cttcgaaaac
     2341 tgaagagggg agacgtggct tggcgccccg gcctcctctc gggtcggcgc tacctttcac
     2401 cccgtttctc ttccccttac agggcgccga cccgagagga ggttgccaga cctctagaga
     2461 gaatcgcttg tatagaatgg acctgagcgc ctcgaggcgc tcaggtctcg cgaggatagg
     2521 aggaggaaga aatggctacc aggaggatgt gcgacgtctg cggcatcagg ccggctgtcg
     2581 tcacggtaag acggataatt cctggagaag gcgagagggt cgagcacctc tgcgagatcc
     2641 acgcggctga ggctggtgtc ctc
//
LOCUS       DASTUV010000043         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_531442, whole genome shotgun sequence.
ACCESSION   DASTUV010000043 DASTUV010000000
VERSION     DASTUV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531442"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFT03_00890"
     CDS             complement(<1..346)
                     /locus_tag="VFT03_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU4846726.1"
                     /translation="MTTRTKNNTVQEVAEFLKTLDRVAITTHVGADGDAIGASAALVR
                     LMRALGAAAIFCHAEEVPSYLRWMLPEEIFRELPQGHDLLVVDTSRPERAGVPVPEAG
                     ARLNLDHHEDNPF"
     gene            complement(324..680)
                     /gene="rbfA"
                     /locus_tag="VFT03_00895"
     CDS             complement(324..680)
                     /gene="rbfA"
                     /locus_tag="VFT03_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU4846727.1"
                     /translation="MSGRTRKVESQLKEIVGEEVSALSDPRIQGLVTVTGVRVSPDLA
                     QATVFYSVLAGADADGAREGLNSAAGRIQAAVGAQTRLKRTPRLRFEPDPVVEQVSRI
                     ENALREVRSVDDPYEE"
     gene            complement(694..975)
                     /locus_tag="VFT03_00900"
     CDS             complement(694..975)
                     /locus_tag="VFT03_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU4846728.1"
                     /translation="MFCTVRLELELPFASSLKEKRQILRSIKDRLRRKNVSVVETDNH
                     DVWQRATVELALAAVSRGAAEEKREEIRRALLNYEELAILDWREEFVKL"
     gene            complement(985..>2370)
                     /gene="infB"
                     /locus_tag="VFT03_00905"
     CDS             complement(985..>2370)
                     /gene="infB"
                     /locus_tag="VFT03_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU4846729.1"
                     /translation="QHIGAYQVESGGKRITFIDTPGHEAFTEMRARGAKVTDIVVLVV
                     AADDGVMPQTQEAIEHARAAGVPIVVAINKIDVPNANLDRVMGELAERELTPEAWGGE
                     TVTVPVSAKTGEGIDELLENILVVAELEDLKANPNAPASGYAIEGERDPGRGPVATLL
                     LNRGTLHKGDVVLAGTAYGRVRAMLDYTGSRVKNAGPGDPAEILGLSGVPEAGTRFEV
                     VDHERIARDRAQQAEAALRRQELAEQGGTRRTLEDLLGESGMQDLNLVVKADVAGSVE
                     ALKEALARLSSDEVRVNIVRSGVGALTDSDVMLASASDGILLGFNVRPTNTAKQVAER
                     EGVEIRTYDVIYKALEEIEAAMRGMLAPETAENETGTAEVRALFRVPGVGTIAGCYVT
                     RGEISRNNRVRVVRDGTVVYEGNIASLKRFRDDVRSVREGFECGIGIENFNDVKEGDV
                     LESFEVVEVPR"
BASE COUNT          354 a          806 c          770 g          440 t
ORIGIN      
        1 agaaggggtt gtcctcgtgg tggtcgaggt tcaacctagc gcctgcctcg gggaccggga
       61 cgccggccct ctcagggcgg gaggtgtcga ccaccaggag gtcatggccc tggggaagct
      121 cgcggaagat ctcctccggg agcatccacc tcaggtagga ggggacctcc tcggcgtggc
      181 agaagatagc cgcggcgcct agagctcgca tcagcctcac gagcgcggcc gaggcgccta
      241 tggcgtctcc gtcggcgcct acgtgcgtcg ttatggcgac gcggtcaagg gttttgagga
      301 actcggctac ctcctggacg gtgttattct tcgtacgggt cgtcaaccga tcttacctcc
      361 ctcaaagcgt tctctatcct ggatacctgt tccacgaccg ggtcgggctc gaagcggagc
      421 ctgggggtgc gcttcaggcg cgtctgagcc cccacggcgg cctggatgcg cccggccgcg
      481 ctgttcaggc cttcccgggc gccgtccgcg tcagccccgg cgaggacgct gtagaagacc
      541 gtggcctgcg cgaggtcggg gctcacgcgg acgcccgtca cggtgacgag cccctggatg
      601 cgggggtcgg agagggcgga cacctcctcg cccacgatct ccttcagctg cgactccacc
      661 ttgcgggtgc gtccgctcac ggcgatagct ccttcaaagc ttcacgaact cctcgcgcca
      721 gtcgaggatc gccagctctt catagttaag tagtgcacgc cggatctcct cgcgcttctc
      781 ctcggccgcg ccccgggaga ccgcggcgag cgcgagctcc acggtggcgc gttgccagac
      841 gtcatggtta tccgtctcga caaccgagac gttcttgcgg cgcaggcggt ccttgatgga
      901 gcggaggatc tggcgcttct ccttcaagct ggaggcgaag ggaagctcca gctcgagcct
      961 gacggtgcag aacaagaacc tggactacct agggacctcg acgacctcga aggactccag
     1021 gacatctccc tccttgacgt cgttgaagtt ctcgatgccg atgccgcact cgaagccctc
     1081 gcggaccgag cgtacgtcgt cgcggaaacg cttcagggag gcgatgttgc cctcgtagac
     1141 cacggttccg tcgcgcacca cgcgaacgcg attgttgcgc gagatctcac cccgggtcac
     1201 gtagcagccc gcgatggtac ccacgcccgg aacccggaag agagcgcgca cctcggcagt
     1261 gcccgtctcg ttctctgcgg tctcgggagc gagcatgccg cgcatggcgg cttctatctc
     1321 ctcgagcgcc ttgtagatca cgtcgtaggt acggatctcc acaccttcgc gctcggctac
     1381 ctgcttggcg gtgttcgtcg gcctgacgtt gaagccgagc aggatcccgt cgctcgccga
     1441 ggcgagcatg acgtccgagt cggtaagcgc cccgacaccg ctgcggacta tgttgacgcg
     1501 gacctcgtcg ctcgagagcc tggccagagc ctccttgagc gcctcgacag atccagcgac
     1561 atcagccttg acgacgaggt tgagatcctg catgccgctc tcgcccagga gatcctcgag
     1621 cgtacggcgc gtcccgccct gctccgcgag ctcctggcgc ctgagggccg cctcggcctg
     1681 ctgggctctg tcgcgcgcga tcctctcgtg gtcaactacc tcgaagcgag tacccgcctc
     1741 gggtacaccc gagaggccaa gtatctccgc cgggtcgccc gggcctgcgt tcttgacgcg
     1801 gctaccagtg tagtcgagca tcgcgcgcac ccggccgtag gcggtgccag cgagtactac
     1861 gtcgcccttg tggagggtgc cgcggttcag aagcagcgtc gccacgggtc cgcggccggg
     1921 gtcgcgctcg ccctcgatcg cgtagccgct ggcgggcgcg ttcggatttg ccttgaggtc
     1981 ctcgagctcg gcgacgacga gtatgttctc gaggagctcg tctatgcctt cgccggtctt
     2041 ggccgacacg gggaccgtga ccgtctcccc accccacgcc tcgggtgtta gctcgcgctc
     2101 cgcaagctcg cccatcaccc tgtccagatt ggcgttgggg acgtctatct tgttgatcgc
     2161 aacgacgata gggacgccgg ccgccctggc gtgctcgatg gcttcctggg tctggggcat
     2221 gactccatcg tcggccgcta ccacgaggac gactatgtcc gtcaccttcg caccgcgggc
     2281 gcgcatctcg gtgaacgcct cgtggcccgg ggtgtcgatg aaggtgatcc tcttcccccc
     2341 gctctcgacc tggtaggcgc cgatgtgctg
//
LOCUS       DASTUV010000044         5886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_673613, whole genome shotgun sequence.
ACCESSION   DASTUV010000044 DASTUV010000000
VERSION     DASTUV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673613"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..399)
                     /locus_tag="VFT03_00910"
     CDS             complement(67..399)
                     /locus_tag="VFT03_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4846730.1"
                     /translation="MDAFVAEELVAGLDPGGHDYAEFFRSSTLSLSVSRWPAGSVDDQ
                     EPHTEDEVYYVVSGRARLQVAEEDAAVGAGSIAYVAAGVEHHFYDIEEDLSVIVFWAP
                     PRHTNAPR"
     gene            746..1537
                     /locus_tag="VFT03_00915"
     CDS             746..1537
                     /locus_tag="VFT03_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4846731.1"
                     /translation="MAESRASGGIMSYRELGEGPGDPVILLHAFPLNGRMFEPQIEAL
                     SAGRRVVAPDYPGFGRSPRTPAQPDVRYYAEAVRRLLDRLGIERVVLGGVSMGGYVAF
                     GCLRLFPEKVSGLILADTRPEPDSEEMRTNRKNMARRVAEEGVEVLIELQMERLLARD
                     TLENDAAVVEKVRAMILESTPNGVVAALGAMRDRPDSTSLLAEIDVPTLVIGGEEDGI
                     SSPEVMGAMAEKIPNARHVTLARAGHLSNLEAPEGFNKALEEFLA"
     gene            1540..2397
                     /locus_tag="VFT03_00920"
     CDS             1540..2397
                     /locus_tag="VFT03_00920"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU4846732.1"
                     /translation="MRGGKILVAGATGRVGGAAVRHLLGAGYEVRALVRRAKKGEPLR
                     ALGAEVSVGDVTRPETLAPALEGCAGVFSVLSAATDRQAVEVEYGGNVNLLSAAREAG
                     AKRFVYSSALHADHPLSQRVGTFREKARFEEVLFGADDVSATVLRPSMFMETLLLALK
                     GPGAFVMGRQRRPVSWISAGDAALAAVRAFERDITGRHEIAGPDTATFDEAYRRLSAA
                     RGERIIVLHPPLSAVRLAGRFAAPVEELANMFALFDAAGYATDPTPLRDVFGVRALTI
                     EEWARRVSG"
     gene            2424..3287
                     /locus_tag="VFT03_00925"
     CDS             2424..3287
                     /locus_tag="VFT03_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4846733.1"
                     /translation="MTRVLHVSDLHFGRPASSERLDSLKELIPKLEPDAVAVSGDLTQ
                     RCSKNEFLQACRYLDEIGEVAPYIVIPGNHDIRWLGAVWRNMGGLFRKQAHEFKYSRY
                     KKFVCEDLTPSLEVPGAVIAGLNTAHGITRGSITRRFRDLGVIGHVRYADIESVREAF
                     ENASLEAARIVMIHHNPIRGELSGRHGLANTETALHTFASLGTELILCGHDHLQAIYN
                     VERGVHGLVISTAGTISNRIRPGRASSFNLVTIDERNLRITTYSWQNPDGFVPSDESS
                     FRRKVATGALE"
     gene            3413..3625
                     /locus_tag="VFT03_00930"
     CDS             3413..3625
                     /locus_tag="VFT03_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846734.1"
                     /translation="MWSRLTRGGDGLPREKVDAIVRLIDQYLAEEASLRNLQSDKQTI
                     HPRDLPPDKREALIDEIFEILKDSKK"
     gene            complement(3638..4486)
                     /locus_tag="VFT03_00935"
     CDS             complement(3638..4486)
                     /locus_tag="VFT03_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU4846735.1"
                     /translation="MMASGEEARVRYGFVIPRGDPRAVAELAGELEAAGWDGAFYWDG
                     INIGDMETYDPWVVMAAMAMRTGRVRIGAMLTPPARRRPWKLARETMTLDRLSGGRLV
                     LPVGLGTLDDGGFSKVGEPTDRRVRARLLDESLDILTGVWSGEPFSYEGEHYRLEEMT
                     FLPRPVQRPRIPIWVVGAWPSEKSMNRALRYDGLLAYTTRGEVTPEDIRAMKAYVEEN
                     RSQDTPFDIIWEGETPGDDPERAAQIVRPFAEAGVTWWIESRWTPPNEPEDLRARIRQ
                     GPPSIG"
     gene            4621..>5886
                     /locus_tag="VFT03_00940"
     CDS             4621..>5886
                     /locus_tag="VFT03_00940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU4846736.1"
                     /translation="MTCAACARRVEKALLGTDGVRAANVNLAAEKATVEYDPASVGLE
                     RLVGAVEDTGYGVVREEQTVDVHAREYAKLRGDFLIAAVLTALIFVGSLPHMLGLTLP
                     VPTTWLNLALLALATPVQFWSGRRFYRGAWGALKHGSADMNTLVVVGTSAAYFYSVVA
                     ALAPQLFAGGMGVGVYFDTSALIVTLILLGRLLEARARGRTSEAIKRLAGLGAKTARV
                     VRDGEEGDVPVEDVSVGDVVVVRPGEKVPVDGVVVSGESAVDEAMISGEPMPVVKRVG
                     DGVIGATINTTGSFRFEATKVGKDTALQQIMRMVEEAQGTKAPIQRLADRISGVFVPV
                     VAAIAALTFVVWLVFGPEPAFTLALLNFVAVLIIACPCAMGLATPTSIMVGTGKGAES
                     GILIKGGEALEAAHKLTTVVLDKTGTLTRG"
BASE COUNT         1032 a         1985 c         1908 g          961 t
ORIGIN      
        1 atcggcgcgt atcggtagat tcagtgaact acggctgtag tgctcgaagc gcgtcgtgca
       61 atcgcatcat cgcggcgcgt tggtgtgcct gggcggcgcc cagaagacga tcacgctcag
      121 atcctcctcg atgtcgtaga agtggtgctc gacgcccgca gcgacgtacg cgatgctgcc
      181 ggcgcccacg gccgcgtcct cctcggccac ctgcagcctg gccctgcccg agaccacgta
      241 gtagacctcg tcctcggtgt gcggctcctg gtcgtccaca gaccccgccg gccagcgcga
      301 gaccgacaaa ctcagagtgg acgagcggaa gaactcggcg tagtcgtgcc cgcccggatc
      361 caggcccgca accagctcct ccgcgacgaa ggcgtccatc gcctaaacct ctcccgcttc
      421 caacgaaccc ccggcgtacg acatgtccgc tcctcctcta cgcgcctcca cagaacttgt
      481 ctctaaacta acctcggaag gggccgcacg caaggaccca tctcctggag cgacgacgtt
      541 aacgctggcg gcgggcgtgt cggcccgcga gggatcgagg cgagctctgg acacttcccc
      601 gggctcgacc gggacgcgct cgcggtggtc ggcatccagg caacgcagcg caggtgggcc
      661 ccaacccgtc ggtcgggatc gctagaatga aggctaaagg cgccagcact cgccttggac
      721 gtgaaacctg aaatgaggag aagacgtggc ggagagcagg gcttccggcg ggataatgtc
      781 gtacagggag ctcggggagg ggcctgggga cccggtaatc ctcctccacg cctttcccct
      841 gaacggcagg atgttcgaac cgcagatcga ggccctctcg gcgggacgcc gcgtagtagc
      901 cccggactat cctggcttcg gccgttctcc ccgcaccccc gcgcagccgg acgtgcgcta
      961 ctacgccgaa gcggtccgca ggctcctcga ccgcctgggg atcgagcggg tggtactcgg
     1021 cggcgtctcg atgggcggct atgtcgcctt cgggtgtctg cggctcttcc cggagaaggt
     1081 atccggcctc atcctcgccg acacccgccc cgagccggac tccgaagaga tgcggaccaa
     1141 ccgcaagaac atggcccgcc gcgtcgccga ggagggggtg gaggtcctga tcgagctgca
     1201 gatggagcgc ctgctcgccc gcgacaccct cgagaacgac gcggcggtcg tcgagaaggt
     1261 gcgcgccatg atcctcgaga gcaccccgaa cggcgtcgtc gcggccctgg gcgccatgcg
     1321 cgaccgcccg gactcgacct cgctgctagc cgagatagat gtccccacgc tcgtcatcgg
     1381 cggcgaggaa gatgggatct cctcccccga agtcatgggg gcgatggcgg agaagatccc
     1441 aaacgcccgc cacgtgaccc tggcccgcgc cggtcacctc tcgaacctcg aagcccccga
     1501 aggcttcaac aaggccctcg aagagttcct ggcgtagcca tgcgaggagg taagatcctg
     1561 gtcgccggtg ccaccggaag ggtgggtggc gcggcggtca ggcacctgct gggggctggc
     1621 tatgaggtcc gcgcgctcgt gcgcagggca aagaagggag agcccctgcg cgcactcggt
     1681 gccgaagtgt ccgtgggtga cgtgacgaga cccgagacgc tcgcgcccgc cctcgaaggt
     1741 tgtgcgggcg tcttctccgt gctctccgcc gcgacggaca ggcaggccgt ggaggtcgag
     1801 tacgggggca acgtaaacct cctctcggcg gcgcgggagg ccggcgcgaa gcgcttcgtc
     1861 tacagctcgg cgctgcacgc ggatcacccg ctgtcccaga gggtcgggac cttcagggag
     1921 aaggcccgct tcgaagaggt gctgtttggc gcggatgacg tctccgcgac agtgctgcgt
     1981 ccgtccatgt tcatggagac gttgctcctg gcgctcaagg ggcccggcgc cttcgtgatg
     2041 ggccgtcagc ggcgcccggt gtcctggatc tccgctggcg acgcggccct cgccgccgtc
     2101 agggccttcg agagggacat aaccggccgc cacgagatcg ccggtcccga caccgccacc
     2161 ttcgacgaag cgtaccgccg cctctcggcg gcacggggag agaggatcat cgtgctccac
     2221 ccgccgcttt cggcggtgcg cctcgccggg cgcttcgcgg caccggtcga agagctggcc
     2281 aacatgttcg ctctcttcga cgccgccggg tacgccaccg accccacgcc gctccgggac
     2341 gtcttcgggg tgcgggcgct caccatcgag gagtgggccc ggcgggtttc cgggtagggc
     2401 ccgttcgggt agaatctttt cccgtgacca gggtactgca cgtctcggac ctgcacttcg
     2461 ggcggccggc ttcctccgag cggctggact ccttgaagga actcataccc aagctggagc
     2521 cggacgccgt agccgtctcc ggcgacctga cgcagcgttg ctcgaagaat gagttcctgc
     2581 aggcctgccg gtacctggat gagatcgggg aggtcgcacc ctacatcgtc atccccggca
     2641 accacgacat ccgctggctc ggggccgtgt ggcgcaacat gggcggcctg ttccgcaagc
     2701 aggcccacga gttcaagtac tcccgctaca agaagttcgt ctgcgaggac ctgacccctt
     2761 ccctcgaggt gcctggcgcg gtcatcgcag gcctgaacac ggctcacggc atcacgcggg
     2821 gttcgatcac aagacgcttc agagacctcg gcgtgatcgg gcacgtcagg tacgcggata
     2881 tcgagagcgt cagggaagcc ttcgagaacg cctcccttga ggccgcccgc atcgtcatga
     2941 tccaccacaa ccccatccgc ggcgagctct cgggccgcca cgggctcgcc aacaccgaaa
     3001 cggcgctgca caccttcgca tctctgggca ccgagctgat cctctgcggc cacgaccacc
     3061 tgcaggccat ctacaacgtc gagaggggcg ttcacggcct cgtcatctcg accgcaggca
     3121 cgatctccaa ccgcatccgc cccggacgcg cctcgagctt caacctggtg accatagacg
     3181 agcgtaacct ccgcataacg acctactcct ggcagaaccc cgacggcttc gtcccctcag
     3241 acgagagctc cttccgcagg aaggtcgcga ccggcgcact cgagtgagtc aataggcatc
     3301 tggtgtcagg ggtggtgtac aggctctcgt tcgctaccgc gaggtccaac acctgaaacc
     3361 tgactgtata atctgccgcg acactgcaac ccgccaagga gggccgtaga ctatgtggag
     3421 cagattgacc cgcggcggcg acggcctccc gcgagagaag gttgacgcca tcgtccgtct
     3481 catagaccag tacctggcgg aggaggcctc cctccgcaac ctccagtcgg acaagcagac
     3541 tattcacccc cgcgatctcc cccccgacaa gcgcgaagcg ctcatcgacg agatcttcga
     3601 gatcctcaaa gacagcaaga agtagaaggc agcagcctca gccgatggaa ggcggcccct
     3661 gccttatgcg ggctcgaagg tcctccggct cgttgggagg agtccacctg gactctatcc
     3721 accacgtaac acccgcctcg gcgaacggac gcacgatctg cgccgcccgc tcggggtcgt
     3781 cgcccggcgt ctccccctcc cagatgatgt cgaagggggt gtcctgcgaa cggttctctt
     3841 cgacgtacgc cttcatcgcc ctgatgtctt ccggcgtgac ctcgccgcga gtggtgtagg
     3901 caagcagacc atcgtagcgc agcgcacggt tcatcgactt ctcgctcggc caggccccta
     3961 ccacccagat cgggatgcgc ggcctctgca ccgggcgcgg tagaaacgtc atctcctcca
     4021 acctgtaatg ctcgccctcg taggagaacg gctccccgct ccagacgccg gtcagtatgt
     4081 ccaggctctc gtccagcaac cgcgcgcgta ccctgcggtc cgtaggctcc ccaactttcg
     4141 agaaaccgcc gtcgtctagc gtccccagac ctaccggcaa taccagccgc ccgccagaga
     4201 gtcggtccag cgtcatcgtc tcccgggcca gcttccaggg acgccggcgc gccggcggcg
     4261 tcaacatcgc cccgatgcga accctccccg tgcgcatggc catcgccgcc atcacgaccc
     4321 acgggtcgta ggtctccatg tcgccaatgt tgatgccgtc ccaatagaaa gcgccgtccc
     4381 aacccgccgc ttccagctcg cccgcaagct cggccacagc gcggggatca cccctgggaa
     4441 tgacgaagcc ataacgcacg cgcgcctcct ctccagatgc catcatcttc tcaaagacac
     4501 ccggcgtgag tatacggaga ccatcgccac ctatactacc cttgtatagg gtaggggggt
     4561 atagtaagct acgatcgcat gagcgaagag tggaaaagcg tatcgctacc tgtcaccggg
     4621 atgacgtgcg cggcgtgtgc gcgcagggtc gagaaggctc tcttgggaac ggatggtgtc
     4681 cgcgcggcca acgtgaacct cgcggccgag aaagcgactg tggagtatga ccctgcctct
     4741 gtgggcctgg agaggctcgt cggcgccgtg gaggatacgg gatacggggt cgttcgggaa
     4801 gagcagaccg tggacgtgca cgcgagagag tacgcaaagc taaggggaga cttcctgatt
     4861 gcggcggttc tgacggcttt gatctttgtc ggcagcttgc cgcacatgct cgggcttacg
     4921 ttgcctgttc ccacaacgtg gctcaacctg gctcttctcg cgctcgcgac gccggtgcag
     4981 ttctggtcgg ggcgccggtt ctaccggggg gcctggggtg ctctcaagca cggtagcgcc
     5041 gacatgaaca cgttggtggt agtcgggacg agcgccgcct acttctacag cgtggtagcc
     5101 gctctggcgc cgcaactctt cgccggtgga atgggggtcg gcgtgtactt cgacacgtcg
     5161 gcgctcatag tcaccctgat cctgctcgga aggctgctcg aggcgcgtgc caggggccgc
     5221 acgagcgagg ccataaagag gctcgccggc ctcggggcga agacggcgcg ggtggtgcgt
     5281 gacggcgaag agggcgatgt gccggtcgag gacgtatccg taggggacgt cgtggtggtc
     5341 cggcccgggg agaaggtgcc ggtcgatggc gtggtggtct cgggcgagtc ggcggtggac
     5401 gaggccatga tctccggcga gcccatgccg gttgtaaaga gggttggcga tggggtgata
     5461 ggggcgacca taaacaccac cggctcgttc agattcgagg cgacgaaggt cgggaaggac
     5521 accgctctgc agcagataat gaggatggta gaggaggcgc agggcaccaa ggcgcccata
     5581 cagcggctgg cggacaggat cagcggcgtc ttcgtgcctg tggtggcggc gatcgcggct
     5641 ctcacgttcg tagtgtggct cgtcttcggc cccgagccgg ccttcacgct cgcgctcctc
     5701 aacttcgtcg cggtcctgat catcgcctgc ccgtgcgcga tggggctcgc gaccccgacc
     5761 tcgataatgg tcggcacagg caagggcgca gaatccggca tcctcatcaa gggcggcgaa
     5821 gctctcgaag ccgcccacaa gctaaccacc gtcgtactcg acaagaccgg caccctcacc
     5881 aggggc
//
LOCUS       DASTUV010000045         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1140720, whole genome shotgun sequence.
ACCESSION   DASTUV010000045 DASTUV010000000
VERSION     DASTUV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140720"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..379)
                     /locus_tag="VFT03_00945"
     CDS             complement(191..379)
                     /locus_tag="VFT03_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846737.1"
                     /translation="MEERDLLLLESAISAIEEAASAVAREVERDRLGESALARLSTVE
                     AELNRSRLALEKIIRDES"
     gene            complement(442..696)
                     /locus_tag="VFT03_00950"
     CDS             complement(442..696)
                     /locus_tag="VFT03_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HEU4846738.1"
                     /translation="MEVVVLILVVIVVLLVLTRGGRYLGQLIVNGIVGVVLLFLTNLV
                     LADDIPINLLTVIICAIGGVVGWLIILILHLLGVAFYVPV"
     gene            complement(824..952)
                     /locus_tag="VFT03_00955"
     CDS             complement(824..952)
                     /locus_tag="VFT03_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846739.1"
                     /translation="MKAKIVIWALSNSRTRGLIIKGLKNRRVRGLALAVAKRRIRR"
     gene            complement(1208..3163)
                     /locus_tag="VFT03_00960"
     CDS             complement(1208..3163)
                     /locus_tag="VFT03_00960"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU4846740.1"
                     /translation="MREGVVSVVSPGAYCVSDGVGDFDGSFGGLFYRDVRHLSRFRFG
                     LGGARLVPLACWVRGSEAEFALAAGVGPEGVDVVRRRSLGDGMTEEILLANESGVAVE
                     VRVELECAADFGDIFEIRGHRRSADRGEVLEEVRDGYLRFAYSREGFRRGTVGRVEGE
                     EIEPLAELGRLSFDVRLGPGEERAVRVSVVLEEDSREVRLGRSAPLYGEAPALETGWE
                     ALRRSWERSVEDLGSLSFDAGEGLVVPAAGAPWYMALFGRDALIAGYQAMVLGPGPAR
                     NALRALARRQARERDDFRDAEPGKILHELRNGELAYFGEIPQTPYYGTVDATPLFLVL
                     LSEVWRWSGDDVFVGELEGAARRALDWILHQADRVSGYVAYATRSTAGLKNHGWRDHG
                     DSMLFRDGTCAGAPIAPCEVQGYVYDAFLRTAELAERVWGDARLAAELGEEAAVLRER
                     FDLDFWMEDRSYYALALDGEGRQVDSVSSNAGHLLWSGIVPEEKVRDVADLLLDERLF
                     GGWGVRTMAAGEGGYDPESYHNGSVWPHDNALIAQGLRRYGFREEANRIVAALIDAAP
                     RFDYRLPEVFAGYARGEVSEPVELPRSCSPQAWAAGTVPLLVRAMLGVEPDTEGRKLL
                     ADPALPEGVGGLRLEGVPAFGERHDVG"
     gene            3302..>3781
                     /locus_tag="VFT03_00965"
     CDS             3302..>3781
                     /locus_tag="VFT03_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4846741.1"
                     /translation="MTEELDRRAQILDAAFEEFSEKGFKGATIKSIARAAGLQSPALI
                     YWYFPDKEALFREVLGNRIPVLRAVRDPASLLDQPPERVLPMIAGRYLSTFDNPAAQR
                     MVRLMIGEAMRRPEIAEIFGNAVIKQVLGFLKSYMARQIELGRLRPHDVRSSARAFMG
                     "
BASE COUNT          708 a         1359 c         1076 g          638 t
ORIGIN      
        1 cgtcgccacc gtcttcgcca gccgcgccaa ctcgaacgac tcgggctacg gcataccctc
       61 ccaactcgtc cagcgccgcc tggccacggc agccggaagc accgaaccag tcaatacagg
      121 ggggtgtgtg agttagctgg cgggcttcgg ggtacagcgc aatcccgaag gccgagagct
      181 ggggtggctc tcagctttcg tcgcggataa tcttctccag cgccagcctg ctccggttta
      241 gctcggcttc aacggtcgag agcctcgcca gggcggactc gcccagccgg tcgcgctcga
      301 cctcccgggc cacggcggac gccgcctctt ctatggcgct gatggcgctc tccagcagca
      361 agagatcccg ctcttccatg aacgggatac tactaccgga gcgcgagcct tgcgtcgttg
      421 cgcttggcga ccatgtgagt gctagacagg aacgtaaaaa gctacgccca atagatggag
      481 tatcaggatg atcagccatc caaccacacc gccgatcgcg cagatgatta cggtgagcaa
      541 attgatgggt atgtcatccg ccagaacgag gttcgtcagg aaaaggagaa ccacccccac
      601 gatcccgttg acgatgagct gccccaggta gcgtccgccg cgggtaagca ccagcaatac
      661 tactattact acgagtatga gtactactac ttccatcaac cgtcattcta aaccagacac
      721 cacagtcttc taacgcagca attgagatgc tttggagctc ccgaggcctc gggccctttc
      781 ttttctgcac cgcccggaac ctcggagtgc agcttacctc gacctaccgg cggatgcgcc
      841 gtttggcaac cgccagggct aacccgcgca ccctccggtt ctttaggccc ttgatgatga
      901 ggccgcgtgt tcgggagttg ctcagcgccc agataactat cttcgccttc atgatgacgg
      961 tcgcctcctc gtctgtttga cctcgtaagg tgagacggca ccgcgaagtt cgccgttaca
     1021 cgaggtgatt cgaatgccct cactcgggcg ccctaccgac cgacaggtcg ggcatcacaa
     1081 gcaccgttct cctccgagta gcttccccag gctctcgaag atctattcat gacccttgca
     1141 tcgtcacccg cgccatcgta ggggttggta atccgaaccg gtacgtgcta gttgtcatcg
     1201 ggcccatcta acccacatcg tggcgttctc cgaaggccgg cacgccttcg agccgcaagc
     1261 ccccaacgcc ctctggcagg gccggatcgg cgagcaactt ccggccttcg gtatcgggct
     1321 cgacgccgag catcgcgcgc acgaggagcg gcaccgtacc cgcagcccac gcctgcgggc
     1381 tgcacgaccg cggcagttcg accggctcgg agacctcgcc ccgcgcgtag cccgcgaaga
     1441 cctcgggcag acggtagtcg aagcgcgggg cggcgtcgat cagcgccgcg acgatacggt
     1501 tcgcctcctc ccgaaagccg tagcgccgga ggccctgcgc gataagcgcg ttgtcgtggg
     1561 gccagacgga gccgttgtgg taggactccg ggtcgtatcc cccctcgccc gcagccatcg
     1621 tcctgacgcc ccaccccccg aacaaccgct cgtccagtag gagatccgcg acgtcccgca
     1681 ccttctcctc ggggactatg ccgctccaga ggaggtgccc ggcattggag ctgacggagt
     1741 ctacctgccg gccctcgcca tccagggcca gggcgtagta gctacggtcc tccatccaga
     1801 agtccagatc gaagcgctcc cgcagaaccg ccgcctcctc tccgagctct gcggcgaggc
     1861 gggcgtcacc ccacacccgt tcggcgagct cggcagtccg caggaaagca tcgtatacat
     1921 agccctgtac ttcgcagggc gctatgggcg ccccggcgca ggtcccgtcg cggaagagca
     1981 tggagtctcc gtggtccctc cagccatgat tcttaagccc cgccgtagag cgggtggcgt
     2041 aagccacgta gccgctcacc cggtcggcct ggtgcaggat ccaatccagc gcccgccgcg
     2101 ccgccccttc gagctccccg acgaacacat cgtcgccact ccagcgccag acctcgctca
     2161 ggaggacgag aaacagcggg gtcgcgtcca cggtcccgta gtagggtgtc tgcgggatct
     2221 caccgaaata ggcgagctcc ccgttcctga gctcgtgcag tatcttgccc ggctctgcgt
     2281 cgcggaagtc gtctctctcg cgcgcctggc gcctggccag cgccctcaga gcgttcctgg
     2341 ccggccctgg ccccagcacc atcgcctgat accccgcgat gagcgcatcc cgcccgaaga
     2401 gcgccatgta ccacggcgcc ccagccgccg gcacgaccag accctccccc gcgtcgaagg
     2461 agagagaccc caggtcctcc acgctccgct cccaactcct ccgcaacgcc tcccaccccg
     2521 tctcgagcgc cggcgcctct ccatacagcg gcgcggacct accgaggcgg acctccctgc
     2581 tgtcctcttc gaggaccacc gagacgcgca cggcccgctc ctcgcccgga ccgagacgga
     2641 cgtcgaacga gagacggccc agctcagcga gcggctctat ctcctccccc tcgacccgcc
     2701 ccacggtccc ccgccggaac ccctcccgcg aataggcgaa gcgcaagtaa ccgtccctaa
     2761 cttcctccag aacctccccc cgatccgccg accggcggtg gccccttatc tcgaagatgt
     2821 ccccgaagtc cgccgcgcac tcgagctcga ccctgacctc gaccgccacc ccggactcgt
     2881 tggcgagcag gatctcctcc gtcatgccat ccccaagaga gcgccgccgc accacgtcca
     2941 caccctcagg tccaaccccg gccgccagcg cgaactcggc ctcagaaccc cgcacccagc
     3001 aggccagcgg aaccagccgc gcacccccta gcccgaagcg gaaccgggaa aggtggcgca
     3061 cgtcccggta gaagagtcct ccgaacgaac cgtcgaagtc ccctacgccg tccgagacgc
     3121 agtaagcgcc cggcgataca acgctaacga ccccttcccg catacgactc cctcccataa
     3181 cccaaccaat gccgaatcat acagcccacc gcttccttca tcttcccacc tcactattga
     3241 cagaacgttc aatcaagagt atgctgtgga ttattgaacg ttcaatcaat cgggagatat
     3301 tttgacggag gagttagatc ggcgggcaca gatccttgat gcggcgttcg aggagttctc
     3361 ggagaagggg ttcaaggggg cgaccatcaa gagcatcgcg cgtgctgcgg ggctacagtc
     3421 gccggctttg atctactggt acttcccgga caaggaggct ctctttcggg aagttctggg
     3481 taatcggatc cccgtgttgc gcgctgttcg ggacccggcc tcgcttctgg accaaccacc
     3541 ggagcgggta ctgccgatga tcgcaggcag gtacctttcg accttcgaca atcctgcagc
     3601 tcaacggatg gtgcggctga tgataggaga ggcaatgagg cggcccgaga tcgccgagat
     3661 attcggcaac gcggtgatca agcaggttct cgggttcctc aagagttaca tggcgcgcca
     3721 gatcgagctc ggcaggctcc gtccgcacga tgtacggtcc agcgccaggg ctttcatggg
     3781 g
//
LOCUS       DASTUV010000046         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_531220, whole genome shotgun sequence.
ACCESSION   DASTUV010000046 DASTUV010000000
VERSION     DASTUV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531220"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /gene="glmS"
                     /locus_tag="VFT03_00970"
     CDS             complement(<1..1425)
                     /gene="glmS"
                     /locus_tag="VFT03_00970"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU4846742.1"
                     /translation="MCGIVGYVGKERCVEVLMDGLKHLEYRGYDSAGLALQVDGRIET
                     IKQAGRLGRLAEALDERNGDLSAAWAGVGHTRWATHGPPNEVNAHPQIGDGVAVVHNG
                     IIENFAELKQELIGRGVDFTSDTDTEVIAHLLAEGIEDGASLREALAAALARLEGSFA
                     VAALSAREPGKVVAARNQSPLVVGLGEYENYLASAVGALLRETHEFLVVENGEIVEVS
                     GSGVGIFTLAGELVCREPFEVDWEAEDVELGGYEDYMLKEIHDQPTALRNTLSGRLDI
                     DGAVDLSELDLDLSSTERVAIVACGTAYHAGLLGKEAIERLARIPVEVAVASEYRYSN
                     PIGDGKTLVVAISQSGETTDTLAAVEAARGFGGRVLAVTNTQGSLITREADAVLLTRA
                     GPEIGVAATKTFTAQISALYLLALGLARVRGVVPEADLLGLGRDLRRAPEKVGEALEI
                     LGPRLDEALGLFAEARCALFLGRGA"
     gene            1472..1621
                     /locus_tag="VFT03_00975"
     CDS             1472..1621
                     /locus_tag="VFT03_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846743.1"
                     /translation="MKGEEIYGRERRFVPGVAAEICRRPDDRPRAETHPPNASMNVCL
                     VNSGP"
     gene            complement(1948..2673)
                     /locus_tag="VFT03_00980"
                     /pseudo
     CDS             complement(1948..2673)
                     /locus_tag="VFT03_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
     gene            2767..3249
                     /locus_tag="VFT03_00985"
     CDS             2767..3249
                     /locus_tag="VFT03_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4846744.1"
                     /translation="MSDERDFTIRPGLREDAAEAARLWMRSAEEHTGHDRIYATAPGA
                     ERVMRRFLADLTSGSHAFLFVAESGGRTVGFISGELREGSPTFRQRTWASVDDVFVEP
                     EHRNRGMGRALLKSVEAWARERGADGISLQVAAANGRGRKFYEDLGFREVSVYEVLEF
                     "
     gene            complement(3292..3684)
                     /gene="pcaC"
                     /locus_tag="VFT03_00990"
     CDS             complement(3292..3684)
                     /gene="pcaC"
                     /locus_tag="VFT03_00990"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEU4846745.1"
                     /translation="MSPDDPLYERGMRVRREVLGDDYVDAALERATGFTTDFQDLITR
                     YAWGEIWSRPGLDRRTRSCITLMALMALGRLEELELHVRAALRNGLSADEIKEVILHG
                     AIYCGAPAANSAFAVARRVLAEHDGGTS"
     gene            complement(3681..>4071)
                     /locus_tag="VFT03_00995"
     CDS             complement(3681..>4071)
                     /locus_tag="VFT03_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4846746.1"
                     /translation="TGQGMDTLVDSVIVRWFTPEFFSSNPEAVEKAARMLRETNPEGY
                     AGCCAAIRDMDLRSRLFTITAPTLVIAGSEDPATPPEHGELIRDSIPGADFEVVPDAS
                     HLANIERPEVVTRAILEHLEPVVRQGR"
BASE COUNT          718 a         1352 c         1313 g          688 t
ORIGIN      
        1 agcgccgcgt cccaggaaca gagcgcagcg cgcctccgcg aagagaccga gggcctcatc
       61 cagccggggc ccgaggatct cgagcgcctc tcccaccttc tccggcgccc gcctgaggtc
      121 cctaccgagc cccagaaggt ccgcctccgg gacgacgccc ctcactcgtg cgagcccgag
      181 ggcgaggagg taaagggcag agatctgggc cgtgaacgtc ttggtcgctg cgaccccgat
      241 ctcgggcccg gccctggtga gcaatacggc gtcggcctcc ctcgttatga gcgagccctg
      301 cgtgttggtc acggcgagaa ctcgtccgcc aaacccccgc gctgcttcca ccgcggctag
      361 agtgtcggtc gtctccccgc tctgggagat ggctacgacg agcgtcttcc cgtcgcctat
      421 gggattcgag taacggtact cgctcgccac cgcgacctcg acggggattc gggcgaggcg
      481 ctctatagcc tctttgccga gcaaccccgc gtggtaggcg gtgccacagg cgacgatagc
      541 gaccctttcg gtgctcgaaa ggtcgaggtc tagctccgag aggtcgacgg cgccgtcgat
      601 gtcgagacgt ccagagagcg tgttgcgcag ggcggtgggc tgatcgtgga tctccttcag
      661 catgtagtcc tcgtagccgc cgagctcgac gtcctcggcc tcccagtcca cctcgaacgg
      721 ctcgcggcaa acgagctctc ccgcgagcgt gaagatacct actccggagc ccgaaacctc
      781 gactatctcg ccgttctcga cgaccaagaa ctcgtgggtc tccctgagca gggctccaac
      841 cgcgctcgcc aggtagttct catactctcc cagacccacg accagcgggc tctggtttct
      901 tgcggcgacg accttgcccg gctcgcgcgc gctcagagcc gcgaccgcga acgagccctc
      961 cagacgcgcc agtgcagcgg cgagcgcctc gcgcagcgac gctccatcct ctatgccttc
     1021 ggcgagcagg tgcgctatga cctccgtatc cgtgtcggag gtgaagtcga cgccgcgtcc
     1081 tatcaactcc tgcttgagct ccgcgaagtt ctcgatgata ccgttgtgga ccaccgccac
     1141 accgtccccg atctggggat gggcattgac ctcgttggga gggccgtgcg tcgcccagcg
     1201 cgtgtgaccc actccggccc aggccgccga gaggtctccg ttgcgttcgt cgagggcctc
     1261 ggccagcctg ccaagacgcc cagcctgctt gatcgtttct atccggccgt ctacctgcaa
     1321 ggctagccca gccgagtcgt agccccggta ttcgaggtgc ttcaggccat ccatgaggac
     1381 ctcgacgcac cgctccttgc caacgtaacc cactatgccg cacacgggca cacctccctc
     1441 tcttcgtcca cgggcaggtc ccgccggcag ggtgaaggga gaggagatct atgggcgtga
     1501 gcggcgtttt gtccccggcg tcgccgccga gatttgtcgc cgccctgacg accgcccgag
     1561 ggccgagacg cacccgccga atgcttcgat gaacgtctgc ctcgtcaact ccggcccctg
     1621 aaaggcacgc cggagtcctg gcgcttggat tgtggttgtt cccctacccc gtaagcctca
     1681 acggtcctgc cgagctcatc ggtcacgctt cgtttctctg gcgcacctcc ccgttcgcct
     1741 tcagaaacac gcgcttaact gtacccggtg ccgggggaaa atggaagggc gatggtaatt
     1801 cgcggcgaag aacccagccg ctcccaaatt accggagtgt tctcattgca acgcctgtag
     1861 cgctcacaag cgctacaagg agcaccagga gaactacgct gtcgatggcg ttggtcaagg
     1921 gatattgcca acgagcacag gcaggttcta gaggaaaccg acctgtatca ccccgagcga
     1981 ggcgagaagt aaaatgagga tgggctggtg gaccaggtag atgaacagcg agttcctccc
     2041 aagcgggagg agaggcttcg tggctacggg tgccttattt gcgaacagcg caggcctcct
     2101 tccgttcccg tagaggacgt tccccgcgaa gaggccgagg agcacgaccc cgaaccaggg
     2161 gagcagcggc ctgtagtcag gcatgacgag gttctcgggc acaacgccga aaggcagcag
     2221 ccacgggctc tccgaagaga agccttcgat ccttatgtag accccgaccg cgataatgga
     2281 gaggccgaga aacaggttcg cgtagcggta cctcaggagg gggtaggcga gtattatgga
     2341 caccccgatc aggtgcagga taccgaacgc gacgacgccc atcccgaaga cgacgaacac
     2401 gacggtcaga gccataccgt aggcgaagat cctgatcccc cttagcaggt acttgccgaa
     2461 gaggctccgg cccggccggg cggcgttcgc cctggagtag ctgatggcca gagagacgcc
     2521 gaccaggaac aggaaggtgc cagccgtagc gtcagcgaag cgcgcccaga accccgaggt
     2581 ggcctcgatg tcgtagccgc cgaagtagtc caggtcgtac atcaggtggt agacgaccat
     2641 catcacgatg gcgaccccgc gggcggcgtc tacctcccag aacctctgct cctgttcggc
     2701 gctgcgctcc gtgcccggct cctctcgtag cgtccaggat catagcgcat gggttaggat
     2761 ttgggggtgt ctgacgaaag agacttcacc atcaggcccg gactccggga ggacgcagcg
     2821 gaggcggccc ggctctggat gcggagcgcc gaggagcaca ccggccacga ccggatctac
     2881 gccaccgccc caggggccga gcgggtgatg cgccgctttc tcgccgacct gacgagcggc
     2941 agccacgcct tcctcttcgt cgcggagtca ggtggacgaa ccgtgggctt tatctcgggc
     3001 gagctgcggg agggctcgcc gacgttccgc cagagaacct gggcctccgt ggacgacgtc
     3061 ttcgtcgagc cggagcacag gaaccgaggt atgggccggg cactcctgaa gagcgtcgag
     3121 gcgtgggcga gggaaagagg cgccgacggc atctccctcc aggtagcggc ggcgaacgga
     3181 cggggccgca agttctacga ggaccttggc ttccgcgagg tctcggtcta cgaggtcctg
     3241 gagttttaaa gcacctggtg tatggcggag gcgctttgcg aagcgccttc tctacgaggt
     3301 cccaccgtcg tgctcggcga ggacgcgccg ggcgacggcg aaggccgagt tggcggccgg
     3361 cgccccgcag tagatcgcgc cgtgtaggat gacttctttg atctcatccg cgctcaggcc
     3421 gttgcgcagc gcagcgcgga cgtgcaactc aagctcctct agcctgccca gggccatcag
     3481 cgccatcaac gttatgcaac tgcgcgtcct gcggtccagt cccgggcggg accaaatctc
     3541 accccaggcg tagcgcgtga tgaggtcctg gaagtcggtg gtgaagcctg tcgcgcgctc
     3601 gagtgcggcg tccacgtaat cgtcgcccag cacctcccgc cgtaccctca tcccacgctc
     3661 gtagagaggg tcgtccgggc tcatctcccc tgcctcacga ctggctccag gtgctcgagg
     3721 atcgcgcgcg taacgacctc gggccgctcg atgttcgcga ggtgagaggc gtccggcacg
     3781 acctcgaagt ccgcgccggg gattgagtcg cggatgagct cgccgtgctc gggcggggtt
     3841 gcggggtcct cggaacccgc gatgaccagc gtcggggccg ttatcgtgaa gagcctgctc
     3901 cgcaggtcca tgtcccggat cgctgcgcag caaccggcgt agccctcggg gttcgtctca
     3961 cgcagcatgc gggccgcctt ctccacagct tcgggattgc tggagaagaa ctccggggtg
     4021 aaccagcgca cgatgacgct gtccacgagg gtgtccatgc cctgccccgt g
//
LOCUS       DASTUV010000047         7748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_463183, whole genome shotgun sequence.
ACCESSION   DASTUV010000047 DASTUV010000000
VERSION     DASTUV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463183"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="zwf"
                     /locus_tag="VFT03_01000"
     CDS             <1..1323
                     /gene="zwf"
                     /locus_tag="VFT03_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU4846747.1"
                     /translation="FRARLHDALKEQRPGRVSEDVWESFSRGIFYLPGDSKKPETYEE
                     LKSFLKQLDEERGTEGNRIYYLSSSPSLFPTIVERLGEAGMNRGENGGYARLVVEKPF
                     GRDLMSARELNQEIRRFFEERQIYRIDHYLGKDTVQNILALRFANIIFEPVWNQHYVD
                     HVQITVAEDIGVGTRGAFYEEAGALRDIVQNHVMQVLCLTAMEPPVTFDAESVREEKV
                     KVLKAVRPIPEDEVENYAVRGQYTRGWIFGEEVPGYREEANVAPDSRTETYVALKLFV
                     DNWRWAGTPIYIRAGKRLPKKVTEIAIQFRPTPHTPFSRGETQGLEPNVLVIRIQPEE
                     GISLKIGAKVPGSGFEISSVNMDLLYGTAFLEEAPDAYQRLLLDLMLGDPTLFIRADE
                     AEDAWAILEPVMRAWSHSKEVPTYPAGTWGPEEADELLERDGRKWRRS"
     gene            1308..2396
                     /locus_tag="VFT03_01005"
     CDS             1308..2396
                     /locus_tag="VFT03_01005"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEU4846748.1"
                     /translation="MAAVVIGETRSSPDGRRMSVEEIEHELARLRMNEDGTLALRASV
                     LNLIVVTDEDSAQSVTRAVSDIAGRYPSRAIVLISDPEEAEKNLEFQLSAFCSVRGES
                     STHVCAEQITIHAEGPPAEHLESLAGPLLIPDLRVFLWYPGPFSPTSPEFAGMVTLAD
                     RIILDSAAADDCELSLKEIAELLDDPETPAVGDLQWVQLTPWRSLVAQIFDPLERAGE
                     LERLRGIEILHAPDGECRALLFVGWLASSLGWSLEGSTRTENGREVRFSGPSGEITTE
                     MSTASPDARFRQVRLYSDDYGFEVARSRDLSEARTVVTRGSEVLGERTVHLGHFDLGV
                     LLGEELSFRGRDGIYENALRAAVEILEP"
     gene            2393..3118
                     /gene="pgl"
                     /locus_tag="VFT03_01010"
     CDS             2393..3118
                     /gene="pgl"
                     /locus_tag="VFT03_01010"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU4846749.1"
                     /translation="MNLLLYETPEELAEAAARDFATRTQEAIYERGSFAVALAGGSTP
                     KAAYEVLARDYANELDWSRVHVSFGDERTVPPDHEDSNYRMAYETLLSRVPVGSVHRM
                     RGELPPDEAAAAYEEELRAFFGQVDVPRFDLILCGLGEDGHTLSLFPETAALDVNDRW
                     VVANPVLKLGTTRLTLTIPVLNASRAVTFLVAGESKAEALKEVLEGDADPRAYPAKLV
                     RPESRDLTLMVDRAAAGLLKPPL"
     gene            complement(3404..4447)
                     /locus_tag="VFT03_01015"
     CDS             complement(3404..4447)
                     /locus_tag="VFT03_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4846750.1"
                     /translation="MERQRVLVTGGAGFLGINLIRHLMTRGYALTSLDVEEFDYPERD
                     RVEVIKGDIRDKSLLDKAIEGADFVVHTAAALPLYSPEDIYTTDVEGTRNVLDAALRH
                     GVKRVVHVSSTAVYGIPDHHPLYETDRLEGVGPYGQAKIQAEMICLEYRARGLTVPII
                     RPKSFVGPERLGVFALLYDWAYTGHNFPMIGSGDNRYQLLDVEDLCDAISLCLTLPED
                     RVNDTFNIGAKEFTTMKEDYQTVLDYAGHGKKVVGFPAAPAIWGLRVLDRLRLSPLYK
                     WVYETASRDSFVSIEKAERELGYAPKYSNKEALLRNFRWYLENRDRFEDSSGVSHRVP
                     WKQGAIGFLKRFF"
     gene            4592..4855
                     /locus_tag="VFT03_01020"
     CDS             4592..4855
                     /locus_tag="VFT03_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846751.1"
                     /translation="MGREDSDLIGRHLREAHESSRGERQLADYVARLDLAECARILEA
                     SPTTLASLAVLREECRVDAGERRTYAQVIRIWTLYVLSGLEGG"
     gene            4864..5850
                     /locus_tag="VFT03_01025"
     CDS             4864..5850
                     /locus_tag="VFT03_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4846752.1"
                     /translation="MVLETPGEPLRVAEVPRPEVGPEDVGIRVHACAVCRTDLHVVDG
                     ELPDPKLPLIPGHQIVGTVEEAGGRVERFARGDRVGIPWLGYIDGTCRYCLSGRENLC
                     DNARFTGYQIDGGYAEHTVADHRFCFPLPEEYPDLQAAPLLCAGLIGYRSLRMTGGAE
                     RLGLYGFGDAAHIIIQVALHQGRKVFAFTRDGDEERQSFARGLGAAWAGGSSELPPEE
                     LDAAIIFAPVGALVPVALRAVAKGGVVVCAGIHMSDIPSFPYEILWGERSVRSVANLT
                     RADGEEFLALAPQVPVRTEVTPFPLEGANEALAALRGGMIEGAAVLVVEQTG"
     gene            5864..6079
                     /locus_tag="VFT03_01030"
     CDS             5864..6079
                     /locus_tag="VFT03_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEU4846753.1"
                     /translation="MPNKRWGDLSSTQRKAVVLSGVVQLGLLAAALADIYRRPAGEIR
                     GPKRLWAAVAFVNYVGPISYFLFGRRR"
     gene            6118..7086
                     /gene="corA"
                     /locus_tag="VFT03_01035"
     CDS             6118..7086
                     /gene="corA"
                     /locus_tag="VFT03_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEU4846754.1"
                     /translation="MIVDSAVYVDGKRVVVSDSLEGVYETYREKDGFAWIGLYEPTRE
                     EFDSVAGEFGLHPLAVRDALRAHQRPKVERYGETLFVVIKAARYVEEKEVVEFGEVHA
                     FVGPDFVVTVRYGETNQMEEVRRSLEGRPDLLRKGPYAVLYAIMDRIVDDYVPVVDGL
                     ANDIDEIEVEVFENQPDVSRRIYELSREVIRFHQATQPLAGTLESLTQEETSDIDREV
                     RGYLRDVQDRVLRVIDQAQGFRELLQNILSVNLTLASVNQNDQVKKISAWAAILIVPT
                     LITGIYGMNFDFMPELHWTYGYPIALLLMLTISLILYWGFKRRGWL"
     gene            7086..7343
                     /locus_tag="VFT03_01040"
     CDS             7086..7343
                     /locus_tag="VFT03_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846755.1"
                     /translation="MEGLRRAWPLLFSLPLIVALVAASLLSGCEGSRPSAQEERQHAE
                     NQSTASGGDRKQASRGGSELRHPVVGDPGAPVLMVEYGDFQ"
     gene            7356..7748
                     /locus_tag="VFT03_01045"
                     /pseudo
     CDS             7356..7748
                     /locus_tag="VFT03_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
BASE COUNT         1414 a         2439 c         2569 g         1326 t
ORIGIN      
        1 ttccgcgcgc ggttgcacga tgccctgaag gagcagcgcc ctggccgggt ctcggaggac
       61 gtctgggagt cgttctccag agggatcttc tacctacccg gcgactcgaa gaagcccgag
      121 acctacgagg agctcaagag cttcttgaag cagctcgacg aggagcgtgg gaccgagggg
      181 aaccggatct actacctctc ctcttccccc tcgctcttcc cgacgatcgt agaaaggctc
      241 ggggaggcgg ggatgaacag gggggagaac ggcggctacg cccggctcgt ggtcgagaag
      301 ccgttcgggc gggacttgat gagcgccagg gagttgaatc aagagatccg gcgcttcttc
      361 gaggaacgcc agatctaccg catagaccac tacctcggca aggacaccgt tcagaacatc
      421 ctggccttac ggttcgccaa catcatcttc gagccggtat ggaaccagca ctacgttgac
      481 cacgtccaga tcacggtcgc cgaggacatc ggggtaggaa cgcgcggcgc tttctatgag
      541 gaagccggcg ctctgcggga catcgtccag aaccacgtga tgcaggtcct gtgcctgacg
      601 gcgatggagc cgccggtgac cttcgacgcc gagtcggtgc gcgaagagaa ggtgaaggtc
      661 ctcaaggctg tcaggcccat cccggaggac gaggtggaaa actacgccgt acgcgggcag
      721 tatacgcgcg gctggatctt tggcgaggag gtccccggct accgcgagga ggcaaacgta
      781 gctcccgatt cccgcacgga gacctacgtc gccttgaagc tcttcgtgga caactggagg
      841 tgggcgggga cgccgatcta catccgggcc ggcaagaggt tgcccaagaa agtaaccgag
      901 atcgccatcc agttcaggcc gacgcctcac acgcccttca gccgcggcga gacgcagggc
      961 ctcgagccca acgtccttgt aatccgaatc cagccggagg agggaatctc cttgaagatc
     1021 ggcgcgaagg tcccgggctc ggggttcgag atcagctcgg tcaacatgga cctcctctac
     1081 ggcacggctt tcctcgagga agcgcctgac gcctaccagc gcctgctcct cgacctgatg
     1141 ctcggcgacc cgaccctctt tatcagggcg gacgaggccg aggacgcgtg ggccatcctc
     1201 gagcccgtga tgcgggcctg gtcccacagc aaagaggtcc cgacctaccc ggccgggacc
     1261 tggggaccgg aagaggccga cgagctcctc gagcgcgacg ggcggaagtg gcggcggtcg
     1321 tgatcgggga gacgcgcagc tcgccggacg ggcggcgcat gagcgtcgag gagatagaac
     1381 acgagctcgc ccggcttcgc atgaacgagg acggcactct agccctcagg gccagcgtgt
     1441 tgaacctgat agtggtgacc gacgaggact cggcccagag cgtgacgcgg gcggtctccg
     1501 acatagcggg ccgctacccc tccagggcga tagtcctgat ctccgacccc gaagaagcgg
     1561 agaagaacct ggagttccag ctctccgcct tctgcagcgt ccgcggcgag agcagcaccc
     1621 acgtctgcgc cgagcagata acgatccacg ccgaaggtcc gcccgcggag cacctggaga
     1681 gcctcgccgg gccgctcctg atccccgacc tgcgcgtctt cctctggtat ccgggtcctt
     1741 tctcgcccac ttcgccggag ttcgccggga tggtgactct ggcggacagg atcatcctgg
     1801 actccgcagc ggcggacgac tgcgagcttt ccttgaagga gatagcagag ttgctcgacg
     1861 accctgagac acccgccgta ggtgacctgc agtgggtcca gctcacgccg tggcgttccc
     1921 tcgtcgcgca gatcttcgac cccctggagc gagctggaga gctggagagg ctgaggggaa
     1981 tcgagatcct gcacgccccc gacggggagt gcagggccct ccttttcgtg ggctggctcg
     2041 cctcctctct gggctggagc ctcgagggat cgacccgcac cgagaacggc cgggaggtca
     2101 ggttctccgg cccctccggt gagatcacaa ccgagatgtc gaccgcctcg cccgacgccc
     2161 gcttccgcca ggtgcggctg tactcggacg attacggctt cgaggtcgcc cgctcgaggg
     2221 acctctccga ggcccgcacc gtcgtcacga ggggcagcga ggtgctcggc gagcgcaccg
     2281 tccacctggg tcatttcgac ctcggcgtct tgcttgggga agagctcagc ttccgcgggc
     2341 gcgacgggat ctacgaaaac gctctgcgcg ccgccgtcga gatactggaa ccgtgaacct
     2401 cctcctctac gagacgccgg aggagctcgc cgaggccgca gcccgcgact tcgccaccag
     2461 gacccaggag gctatatacg aacggggcag tttcgccgtc gcgctcgccg gcggctcgac
     2521 cccgaaggcc gcctacgagg tgctggccag ggactacgcc aacgaactcg actggagcag
     2581 ggtacacgtc tcattcgggg acgagcggac ggtccccccc gaccacgagg actcgaacta
     2641 ccggatggcc tacgagaccc tcctctcgcg cgtcccggtc gggagcgtcc acaggatgcg
     2701 cggcgagttg ccccccgacg aggccgccgc ggcctacgag gaggaactgc gcgcgttctt
     2761 cggacaggtc gacgtcccga ggttcgacct catcctgtgc ggcctcgggg aagacggcca
     2821 caccttgagc ctcttcccgg agaccgcggc tctcgacgtt aacgacaggt gggtcgtcgc
     2881 caacccggtg ctcaagctcg ggaccacgcg gctgaccctc accatcccgg tactgaacgc
     2941 gtcgagggcg gtcaccttcc tggttgcggg ggagagcaaa gccgaggcct tgaaggaggt
     3001 cctggagggc gacgccgacc cgcgtgcgta cccggcgaag ctcgttcgcc cggagagccg
     3061 agacctcacc ttgatggtcg atcgggcagc cgccggattg ttgaaacctc ctctctgaac
     3121 aggttctctc ccatgaaccg cggcgttcgt tgttctccag atcgtagcgg ccggtgttgg
     3181 atcgggaacc tgtaacgctg gccaggatgg gggatcctac aaggaaaagg cgggtcggct
     3241 tgcttttgtg acgtgaaagc agctgaagag cgaaaaaata cagctccgcc gacccgcctt
     3301 ttccatcata cgctaacaat agtaaacttg gatcactttc gggtccgatt cgccaaaaag
     3361 aagctcgaaa agggcgaagt ggctccactc taccccacag aaatcagaaa aagcgcttga
     3421 gaaacccgat cgcaccctgc ttccagggca cccggtgcga gacgccggag gagtcctcga
     3481 acctgtcgcg gttctcgagg taccaccgga agttcctcaa gagcgcctcc ttgttcgagt
     3541 acttcggcgc gtagccaagc tccctctctg ccttctctat ggagacgaag gagtccctgg
     3601 aggcggtctc gtacacccat ttgtaaagcg gcgagagacg aagtctgtcg agcaccctga
     3661 ggccccagat cgcgggcgcc gcggggaagc cgacgacctt cttgccgtgc ccggcgtagt
     3721 cgaggacggt ctggtagtcc tccttcatcg tggtgaactc cttcgccccg atgttgaagg
     3781 tgtcgttgac ccggtcctcc gggagcgtga gacacaggga gatggcgtcg cagaggtcct
     3841 cgacgtcgag gagctggtag cggttgtcgc cgctcccgat catggggaag ttgtgcccgg
     3901 tgtacgccca gtcgtagagg agcgcgaaga cgccgaggcg ctctgggccg acgaaggact
     3961 tggggcggat gatggggacc gtgagacctc tggcccggta ctcgaggcag atcatctccg
     4021 cctggatctt ggcctgccca tacggaccaa cgccttccag tctgtcagtc tcgtacaacg
     4081 gatggtggtc cggtatgccg tagaccgcag tagaggagac gtgcacgacg cgcttcaccc
     4141 cgtgacgaag agccgcgtcg agcacgttgc gcgtcccttc gacgtccgtc gtgtagatgt
     4201 cctcggggga gtagaggggc agggccgcgg cggtgtggac gacgaagtcg gcgccctcta
     4261 tcgccttatc caggagggat ttatcgcgga tgtccccttt gatcacctcg acgcggtccc
     4321 tctccgggta gtcgaactcc tccacgtcga gggaggtcag ggcgtagccg cgcgtcatga
     4381 ggtgccggat caggttgatc cccaggaaac cggcgccccc ggttacgaga actctctgtc
     4441 gttccataga atagaaatct ccttgtgctc tctacgggat tggtacatcc gcgcaaacgg
     4501 cgcgcattgt agcatgcatg gccgccagcc taccggcgcg tcaccgcccg ggggtaaact
     4561 tgtctcagag tacccgagaa gggaaggtat catgggaaga gaggacagcg acctgatcgg
     4621 ccggcacctc agggaggcgc acgaaagctc ccggggcgag cgccagcttg cggattacgt
     4681 ggcgcggctc gacctcgccg agtgcgcgag gattctggaa gccagcccta cgaccctggc
     4741 ttctctggcc gtcctgcgcg aggagtgccg cgtcgacgct ggtgagcggc ggacctacgc
     4801 gcaggtcatc cgcatctgga ccctgtacgt cctctcggga ctggagggtg gatagtgcgc
     4861 gctatggtac tggaaacccc tggcgaaccg ctgcgcgtcg ccgaggtgcc ccgtccggag
     4921 gtggggccgg aggacgtcgg gatccgggtc cacgcctgcg cggtgtgccg gaccgacctg
     4981 cacgtggtgg acggcgagct ccctgatcca aagctacccc tgatccccgg ccaccagatc
     5041 gtcggtactg tcgaagaggc cggcggccgc gtcgaacggt tcgcgcgggg ggacagggtg
     5101 ggcataccct ggctcggcta cattgatggc acctgccgct actgcctctc gggccgggag
     5161 aacctctgcg acaatgcccg gttcaccggc taccagatag acggcggcta cgcggagcat
     5221 acagtagccg accaccggtt ctgcttcccg ctccccgaag aataccctga ccttcaggcg
     5281 gcgccgcttc tgtgcgcagg cctgatcggc taccgctccc tcaggatgac cggcggtgcc
     5341 gaacgcctcg gcctatacgg cttcggcgac gccgcccaca tcatcatcca ggtagctctg
     5401 catcagggcc ggaaggtctt tgcgtttacc agggatggcg acgaggagcg ccagagcttc
     5461 gcgcgtgggc tgggcgccgc ctgggccggg ggctcgtccg agcttccgcc ggaggagctc
     5521 gacgcggcga tcatcttcgc gccggtcgga gcgctggttc cggtcgcgct gcgggctgta
     5581 gcgaaggggg gtgtagtggt gtgcgccggc attcacatga gcgacatacc ctcgttcccg
     5641 tacgagatcc tctggggaga gcgcagcgtg cgctcggtag cgaaccttac ccgcgcagac
     5701 ggcgaggagt tcctggcact cgccccgcaa gtgcccgtcc gcacagaggt gacgcctttt
     5761 ccactcgaag gcgcgaacga ggccctcgcc gcgttgcgcg gcggtatgat agaaggagcg
     5821 gccgtgctcg ttgtcgagca gaccgggtag agaggaggct gccgtgccga acaagaggtg
     5881 gggcgatcta agctccacgc agaggaaagc tgtcgtgctc tcgggggttg tccagctcgg
     5941 gttgctggcc gcggcgctcg ccgacatcta ccggcgtccg gccggggaga tccggggccc
     6001 aaagcggttg tgggcggcgg tggcgttcgt taactacgtc ggacccatct cttacttcct
     6061 gttcggacgc aggcgttagc atccttcttg taggcgctgc ggcggtatca tttggccatg
     6121 atcgttgaca gcgcagtcta cgtggacggg aagagagtgg tagtgtccga ctccctcgag
     6181 ggcgtctacg agacctaccg cgagaaagat ggcttcgcct ggatcggcct ctacgagccg
     6241 accagggagg agttcgactc agttgccggc gagttcgggt tgcacccgct ggcggttagg
     6301 gatgccctgc gagcccacca gcggcccaag gtcgagcgct acggcgagac gctcttcgtg
     6361 gtgatcaaag ctgcccgcta cgtcgaggag aaggaagtgg tcgagttcgg cgaggtccac
     6421 gctttcgtag gacccgactt cgtcgtgacg gtccgctacg gtgagacaaa ccagatggaa
     6481 gaggtgcgcc ggtcgctcga gggcagaccc gatttgctcc gcaagggacc ttacgcggtc
     6541 ctctacgcga tcatggaccg catcgtagac gattacgtcc ctgtggtcga cggcctcgcg
     6601 aacgacatcg acgagatcga agtcgaggtc ttcgaaaacc agcccgacgt ctccaggcgc
     6661 atctacgagc tctcccgcga ggtcatccgg ttccaccagg ccacgcagcc gctggccggg
     6721 acgctggaga gtctcacgca ggaggagacg agcgacatcg accgcgaggt gcgcggctat
     6781 ctgcgcgacg ttcaggaccg cgtgctgcgc gttattgatc aggctcaggg cttccgggag
     6841 ctcctgcaga acatcctgag cgtcaacctt actctcgcga gcgtcaacca gaacgatcag
     6901 gtaaagaaga tcagcgcctg ggccgccatc ctgatcgtac cgaccctcat aacagggatc
     6961 tacggtatga acttcgattt tatgccggaa ctgcactgga cttacggata ccctatagcg
     7021 ctcctcctga tgctcaccat ctctctgatc ctctactggg gcttcaaacg ccgcggctgg
     7081 ctctgatgga aggcctgaga cgagcatggc cgctgctgtt ctcgctgccg ttgatcgtag
     7141 cgctcgtcgc cgcctcgctc ctctccggct gcgagggctc ccggccctcc gctcaggagg
     7201 aacgtcagca cgcagagaac cagagcacgg catcaggcgg cgacaggaag caagcatccc
     7261 gcggcgggag cgaactccgg catcctgtag tgggagaccc tggcgcacct gtgctaatgg
     7321 tcgagtacgg ggacttccag tgaccgttct gcggcaagtt cgcccgtgag gtcgaaccga
     7381 aactcgtgca ggagtacgtc gaggaaggga cgctcaggat cgagtggcgg gacttcccgt
     7441 atctcgggca ggaatctgtg aacgcggcga tggccgccag ggctgcgcag gcgcagggga
     7501 agttctggga gtaccacgac ctgctctacc agaaccagtc aggcggattc ccggacgaga
     7561 agctcgtagc gctggcgcga gaggccggcc tcgacgagaa ggggttcgag gctgacctcg
     7621 cctctggcga gtacggggag gtaatcgccg atgacttccg agaaggtcaa caacgcggca
     7681 tctcgggcac cccgacgttc gtcattaacg gcgaggtgct ggcggggggc cagcctctcg
     7741 aggtcttc
//
LOCUS       DASTUV010000048         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1607749, whole genome shotgun sequence.
ACCESSION   DASTUV010000048 DASTUV010000000
VERSION     DASTUV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607749"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFT03_01050"
     CDS             <1..460
                     /locus_tag="VFT03_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846756.1"
                     /translation="VEKMTAAWKTYEAGEVPKMIEPRMTGALAALARAVGAGDAAQAQ
                     QAAIDAAQWSLDLQLRYRPPAEIDLARFDLWAAQLTLDASAGDAGAVGGDVFTIGYIR
                     DRILNTLVGTDVVRVDTEVQKLQVAAADHDLAAATDAAKRLRDILGQHQG"
     gene            537..1364
                     /locus_tag="VFT03_01055"
     CDS             537..1364
                     /locus_tag="VFT03_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846757.1"
                     /translation="MRKSVATLARSAAALALVAGLVAMSACGSEPTNGGAGGDTETSE
                     KEAPPYAPEVAEENAANYSPQIVPSNFVDKIDNRYMKFTPGTTFVYEGKTADGPERVE
                     TYVSHKTREVLGVKCTVVRDKAFLNGKLSEDTFDWYAQDSEGNVWYFGEDTKEYENGK
                     VVSTKGSWEAGVAGAKPGIVMEANPRVGDSYRQEYYEGEAEDMAEVLSLDASGLNDAV
                     SVPYGSFGDVLMTKEWNPLEPGVLEYKYYAPGTGLIGEEQILGGTDTSELVDIRTGR"
     gene            1477..>3135
                     /gene="thrS"
                     /locus_tag="VFT03_01060"
     CDS             1477..>3135
                     /gene="thrS"
                     /locus_tag="VFT03_01060"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU4846758.1"
                     /translation="MSPVDRADPSYKLRHSTAHVMAQAVKEMLPEAKLGWGPPHDRFA
                     NGFYYDFDLPRALTPQDLPKIETRMRRIIEADHAFRYRVVSADEARELFKEQPYKLET
                     IDDLVAGRDEYGEARSGGPIISTYSQDTFEDLCLGPHLERTGQIPLDGFKLLDVSGAY
                     WRGSEENKMLQRVYGTVWPRKEDLERYLWQREEEEKRDHRRLGSELDLFSIQPEVGPG
                     LALFHPKGALVRTLMEDYWRNRHRAGGYDFVVSPHIGRSVLWDTSGHLRWYRDGMYAP
                     IDIEDEHYYLKPMNCPFHMLIFNSRIRSYRDLPMRFAELGTVYRYEPSGTLRGLLRVR
                     GFTQDDAHLFCRPDQTPAEIERVLDFCLSVLDAYGFTNPEMELSVRDPRTPEKYAGSD
                     DDWVMAESTLAAALDSHGLPYERMEGEAVFYGPKIDIKILDALGRRWQASTIQFDFNL
                     PERFDLTYVGADGQPHRPYMVHRALYGSIERFLALLIEHHSGAFPVWLAPTQAMVIPI
                     TDRNLDYARRVAGELAAAGLRVEVDDRAERMNPKIRDAQLQKIPY"
BASE COUNT          655 a          986 c         1007 g          487 t
ORIGIN      
        1 ggtcgagaag atgaccgcag cctggaagac ctacgaggcg ggcgaagtgc cgaagatgat
       61 cgagcctcga atgaccggcg ccctcgcggc gctcgccaga gcagtcggcg ccggcgacgc
      121 cgcgcaggcc caacaggcag cgatcgacgc cgcccagtgg agcctcgacc tccaactgcg
      181 ataccggcct ccggccgaga tcgaccttgc ccgcttcgac ctctgggccg cccagctcac
      241 gctcgacgcg tcagccgggg acgcgggcgc agtcggcggc gatgtcttta ccatcggcta
      301 catccgggac cgtatcctga acaccctggt tggtactgat gtcgtccgcg tcgacaccga
      361 ggtccagaag ctccaggtgg cggcagccga ccacgatctc gccgcagcga ccgacgccgc
      421 caagcgcctc cgggacatcc ttgggcaaca tcaagggtga gctgaggcgc tgtcaggcac
      481 gcgtcaggca aatgacacag agccttggat tcaaggcaac cgaaagggag gagacgatga
      541 gaaagagtgt ggcaaccctg gcccgcagcg cggcagcgct ggcgctggtg gcaggtctgg
      601 tggcgatgag cgcgtgcggg agcgagccga cgaacggagg tgcgggaggg gacacagaaa
      661 cttcggagaa ggaagcgcca ccgtacgcgc cggaggtagc cgaagagaac gcggcaaact
      721 actcgccgca aatagttcct tctaacttcg tcgataagat agacaaccgc tacatgaagt
      781 tcacgccagg cacgacgttc gtgtacgagg gcaagacggc tgacggcccg gaacgcgtcg
      841 agacctacgt cagccacaag acaagagaag tgctcggcgt gaagtgcacc gtcgtcaggg
      901 acaaggcgtt cctgaacggg aagctgagcg aagacacctt cgactggtac gcccaggaca
      961 gtgaaggcaa cgtctggtac ttcggcgaag acaccaagga gtacgagaac ggcaaggtcg
     1021 tcagcaccaa gggctcgtgg gaagccggcg tggccggcgc gaagccgggg atcgtaatgg
     1081 aggcaaaccc gcgggtagga gactcatacc gccaggagta ctacgagggc gaggccgagg
     1141 acatggccga agtgctcagc ctcgacgcct ccggactcaa cgacgccgtc tccgtcccct
     1201 acgggtcctt cggcgacgtc ctgatgacca aggagtggaa cccgctcgag cccggcgtcc
     1261 tcgagtacaa gtactacgcg cccggtacgg ggctcatcgg agaagagcag atcctgggcg
     1321 gaacggacac gagcgagctg gtggacatca ggaccgggcg gtagcagcac catcgcgcga
     1381 gcttgatccg gccgcaagcc agggatgctg tatcctttgg tcgtcgatct ctatatcgtg
     1441 aaacgatcgc agagagcgcg gtgaaggagt ggtcccatgt ctcccgtaga tcgtgcggat
     1501 ccgtcataca agttacgcca ttccacggcc cacgtgatgg cgcaagccgt caaggagatg
     1561 ctcccggagg ccaagctggg ttgggggccg ccccacgaca ggttcgcgaa cggcttctac
     1621 tacgacttcg acctgccccg tgcgcttacg ccccaggacc tccctaagat agagacgcgc
     1681 atgcgccgga tcatcgaggc cgaccacgcg ttccggtacc gggtcgtcag cgccgatgag
     1741 gcccgcgaac tgttcaaaga acagccatac aagctggaaa ccatcgacga tctggtggcg
     1801 ggaagagatg agtacggcga agcccgctcc ggcggcccca ttatctcgac gtacagccag
     1861 gatacgttcg aagacctctg tctgggacct cacctggagc gcacaggcca gatccctctt
     1921 gacggcttca agctgctcga cgtctcgggg gcatactggc gggggagcga ggagaacaag
     1981 atgctccagc gcgtctacgg aacggtgtgg ccccgaaagg aggacctgga gcgctacctc
     2041 tggcagcgcg aggaagagga gaagcgcgac caccgccgtc tcggcagcga gctcgacctg
     2101 ttcagtatcc agcccgaagt cggtccgggg ctggccctct ttcatccgaa gggcgcactt
     2161 gtccgcacgc tcatggagga ttactggcgc aatcggcacc gcgccggcgg ctacgatttc
     2221 gtcgtttcgc cccacatcgg gcggtcggtg ctctgggata ctagcggtca cctgaggtgg
     2281 tacagggatg gaatgtacgc gccgatcgac atagaagacg agcattacta cctcaaaccg
     2341 atgaactgcc cgttccacat gctgatcttc aacagccgga tacgcagcta ccgcgatctg
     2401 cccatgcgct tcgcggagct cggcaccgtg taccgctatg agccttcagg caccctgcga
     2461 gggctgctac gcgttcgcgg cttcacgcaa gacgacgccc acctgttctg caggccagac
     2521 cagacgccgg cggagatcga gcgcgtcctg gacttctgcc tgtcggttct ggacgcctac
     2581 ggtttcacca acccggagat ggagcttagc gtccgagatc cacggacgcc ggaaaagtac
     2641 gctggcagcg atgacgactg ggtgatggca gaatctacgc tcgcggcggc cctcgattcg
     2701 cacggtctgc catacgagcg tatggagggc gaggccgtct tctatggccc caagatcgac
     2761 atcaagatcc tcgacgctct gggccgccgc tggcaggcgt cgaccatcca gttcgacttc
     2821 aacctcccgg agaggttcga cctgacctac gttggcgctg acggccagcc ccaccgcccg
     2881 tacatggtgc atcgcgccct gtacggctcg atcgaacgtt tcctggcgct cctgatcgaa
     2941 caccattccg gggcgttccc cgtctggctg gcaccgaccc aggcgatggt catacctatc
     3001 acagaccgta acctggacta tgcccggaga gtggccggcg agctggccgc ggccggactg
     3061 cgggtcgagg tagacgatcg tgccgaacgc atgaacccga agatccgcga cgcccagctc
     3121 cagaagatcc catac
//
LOCUS       DASTUV010000049         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_65350, whole genome shotgun sequence.
ACCESSION   DASTUV010000049 DASTUV010000000
VERSION     DASTUV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65350"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..830)
                     /locus_tag="VFT03_01065"
     CDS             complement(129..830)
                     /locus_tag="VFT03_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4846759.1"
                     /translation="MSEILIVEDDEVITSTLAYNLSRHSFGVKAATTGAEALRMARKM
                     RPDLILLDVMLPGESGIEVCERIRERDQDVVIVMVTAKDAEEDKVRGFEAGADDYVTK
                     PFSMKELVARINANLKRSEAGQRGKVIEAGDLQLDTKNFTAHVGGEPLDLRLKEFELL
                     VALASAPGELKSREELAKEVWGHAGVGSSRTIDVHIRRIRAALEERSSYQYIHTARGL
                     GYRFEAKSRAGVGAE"
     gene            996..1991
                     /locus_tag="VFT03_01070"
     CDS             996..1991
                     /locus_tag="VFT03_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEU4846760.1"
                     /translation="MEKRVRTVTVHTRRTFAIARSAAGAFERVILELEEDGAVGRGEA
                     APTRYYGQDAASVAEALRNLEIPDPWDIEGALAHNDCMPTTARTALDSALHDLAAKRL
                     GIPLYRLLGLARPEPVSAYTLGIAEPETTGEEARRLSDFPILKMKVGGREDLETVRAV
                     AQFSEAEIWVDANEAFSPDEAPEVAAELKRIGVRVIEQPVPASAGPEALRLATEAAHP
                     VPVVADESAIVASDVPPLSGCVSGVNVKLAKCGGVRGALAVIHTARALGMAVMLGCMV
                     ETSLGISTAAHISGLVDLVDLDGAMLLADDPFEGIGYENGRILLSGEPGLGARPR"
     gene            2074..2580
                     /locus_tag="VFT03_01075"
     CDS             2074..2580
                     /locus_tag="VFT03_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEU4846761.1"
                     /translation="MSESAQTPMKDVVRDAMGEQIKHELYSAYLYLSMAGSFEVANLT
                     GFAHWMRQQSREELEHAMKFFDFLLDRGERVELQAIEAPPYAFRSPLDTFEQALEHEK
                     EITARINTLYELAVQENDFPAQVMLNWFVTEQVEEEKSATEVAERLRMAGEDSAALLL
                     LDGELGNR"
     gene            2664..3068
                     /locus_tag="VFT03_01080"
     CDS             2664..3068
                     /locus_tag="VFT03_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846762.1"
                     /translation="MAESSGFKAAVLAGYSVRRRVVRGADGMVSVLWGSLGLRMSHAE
                     FVDLAGVLAQAVRSPARYGEMARGVHARVVRCSMGQFTLHHGDLTLWFSPEEFEEIAN
                     LAIRARHKLADSAPAPRLGIPWAPREELFGPN"
     gene            3170..>3551
                     /locus_tag="VFT03_01085"
     CDS             3170..>3551
                     /locus_tag="VFT03_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU4846763.1"
                     /translation="MARCLTLNASYEPVALVPAKRAIVLVVSEKAEIVEANLERRFRS
                     ERAEYPYPLVIRLIKFVEIPRRLRRHVTNAILFARDNYKCQYCGKHRDDLNRRDRLTR
                     DHVKPISRGGANTWDNVVTACTKCN"
BASE COUNT          589 a         1127 c         1195 g          640 t
ORIGIN      
        1 gctcgtcgca ggggtcttga gctcgtggga gacgttcgcg acgaagtcgc gccggatctt
       61 gttgacccgc cgcagctcgt cggggctgac gtaatcggcg tcctcgcgtc tcttgagcac
      121 ggcgatctct actcggcgcc cactccggcc cggctcttcg cctcgaagcg gtagccgagg
      181 cccctggctg tgtggatgta ctggtaggag gatctctcct ccagcgccgc gcggatcctg
      241 cgtatgtgga cgtctatggt gcgcgaggag cccacgccgg cgtgtcccca gacctccttc
      301 gccagctcct cccggctctt gagctcgccg ggcgcgctcg cgagcgccac gagtagctcg
      361 aactccttca ggcgcaggtc cagcggttct cctccgacgt gggcagtaaa gttcttggtg
      421 tcgagctgga ggtcgccggc ctcgatgacc ttgccccgct ggccggcctc gctcctcttc
      481 aggttggcgt ttatccgggc gacgagctcc ttcatgctga aaggcttggt cacgtagtcg
      541 tcggcgccgg cctcgaagcc cctgaccttg tcttcctcgg cgtccttggc cgtgaccatg
      601 acgatcacca cgtcctgatc ccgctctctg atgcgctcgc agacctctat gcccgactcg
      661 ccggggagca tgacgtcgag gaggatcagg tccggcctca tcttgcgggc catcctcaat
      721 gcctccgctc cggttgtagc cgctttcacg ccgaagctgt ggcgcgagag gttgtaggcg
      781 agggtgctgg tgatgacctc gtcgtcctcg actatcagaa tctctgacac gctcaatgca
      841 agcctctcct actcgtcggg cccaggcaaa cgcaagtata aaccgtcgtt ttccggcgct
      901 tcacgttttt aacgagcatt taacagcggt ggccaccgcg gacgtgcgtg ggtgaggtag
      961 gatgcggcgg tcggcgagag ggaggaaagc ttatcttgga gaagagggtc cggaccgtga
     1021 cggtgcatac ccggcgcacg ttcgccatcg cccgcagcgc ggccggcgcc ttcgagcgcg
     1081 tgatcctcga gctcgaggag gatggcgccg ttggccgggg cgaagctgcg cccacgcgct
     1141 actacggcca ggatgcggcg agcgtggcgg aggcgctgcg gaacctcgag atcccggacc
     1201 cctgggatat cgagggcgcc ctcgcgcaca acgactgtat gcccacgacg gcgcggacag
     1261 cgctggactc ggcgctccac gacctcgccg cgaagaggct cggcatcccc ctctacaggc
     1321 tactggggct cgcccgtccc gagccggtca gcgcctacac tctggggata gcggagcccg
     1381 agaccaccgg cgaggaggcc aggaggctct cggatttccc tatcctcaag atgaaggtag
     1441 ggggacggga ggacctcgaa accgttcgcg cggtggcgca gttctcagaa gcggagatct
     1501 gggtcgacgc gaacgaggcc ttctccccgg acgaagctcc cgaggtggcg gcggagctca
     1561 agaggatcgg ggtgcgtgtg atcgagcagc ccgtccctgc ctccgccggg cccgaagcgc
     1621 tccggctcgc gacggaggcc gcccatcccg tgccggtcgt cgccgacgag agcgccatag
     1681 tcgcgagcga cgtgcctccc ctctccgggt gcgtgagcgg ggtcaacgtc aagctcgcca
     1741 agtgcggcgg ggtgcggggc gccctggccg tcatccacac cgcccgcgct ctgggtatgg
     1801 ccgtgatgct cgggtgcatg gtcgagacct cgctagggat ctccacggcg gctcacatct
     1861 ccggcctcgt agacctggtc gacctcgacg gcgcgatgct cctcgcagac gacccgttcg
     1921 aaggtatcgg gtacgagaac gggcgcatcc tcctctccgg ggagccgggg ctcggggcgc
     1981 ggccgcggta ggtttcgaga gccgtctccg ctactcacct ggtactatat gatgcgaaag
     2041 cgagatccgg tgccagaggg tgagaggagc ggcatgagcg agagcgcgca gacgccgatg
     2101 aaggatgtgg tcagggacgc gatgggggag cagatcaagc acgagctcta ctccgcttac
     2161 ctttacctct cgatggcagg ttcattcgag gtggccaacc tcactggctt cgcccactgg
     2221 atgcgccagc agagcaggga agagctggag cacgcgatga agttcttcga cttcctcctc
     2281 gaccgtggcg agcgcgtcga gcttcaggcc atagaggcgc ctccttacgc cttccggtcc
     2341 ccgctcgata ccttcgagca ggccctggaa cacgagaaag agatcaccgc ccgcatcaac
     2401 actctctatg agctcgccgt gcaggagaac gacttcccgg ctcaggtgat gctcaactgg
     2461 ttcgtcaccg aacaggtcga ggaagagaaa tccgccaccg aggtcgccga acgcctcagg
     2521 atggccggcg aggacagcgc cgccctgctc ctgctggacg gcgagctggg taaccgctag
     2581 gtagagcgct tgaaccgcgc tccacctgtg gagtatgatc cttgctgcaa gtggttctca
     2641 tttaggtgga acggtgggga aggttggctg agagttcggg gttcaaagct gcggtgctgg
     2701 cgggctacag cgtgcgtcgc agggtggtgc ggggcgcgga cggcatggtg agtgtcctgt
     2761 ggggatctct ggggctcagg atgtcccacg cggagttcgt ggaccttgcg ggggtgctcg
     2821 cgcaggcggt gaggagcccg gcgcggtacg gtgagatggc gcggggcgtt cacgcgcggg
     2881 tggtccgctg ctccatgggg cagttcacgc tccatcacgg ggacctcacc ctctggttct
     2941 ctcccgagga gttcgaggag attgcgaacc tggcgatccg ggcccggcac aagcttgcgg
     3001 actcggcgcc cgcgccgcgt ctggggatac cgtgggcgcc gcgcgaagag ctctttgggc
     3061 ccaactagcc gttgggggct gtctggcctc gaaccgtaaa agatgtgtta aatggcctaa
     3121 gtatggggtt ttgcacttta caagcatccc gatatgttag aatttcgccg tggcacgatg
     3181 cctgacgctt aacgccagct acgagcctgt ggccttggtg ccggccaaga gggcgatcgt
     3241 cctcgtcgtc tccgagaagg ccgagatcgt cgaggccaac ctggagcgcc gcttccggtc
     3301 cgagagggcc gagtatccct atccactcgt gatccggctc atcaagttcg tcgagatccc
     3361 gcgtcgcctc aggcgccacg tgacgaacgc gatcctcttc gccagggaca actacaagtg
     3421 tcaatactgc ggcaagcacc gcgacgacct gaaccgccgc gaccggctga cgcgcgacca
     3481 cgtcaagccg atctcgcgcg gcggagcgaa cacctgggac aacgtggtta cggcctgcac
     3541 caagtgcaac g
//
LOCUS       DASTUV010000050         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1495930, whole genome shotgun sequence.
ACCESSION   DASTUV010000050 DASTUV010000000
VERSION     DASTUV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495930"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFT03_01090"
     CDS             complement(<1..339)
                     /locus_tag="VFT03_01090"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563599.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU4846764.1"
                     /translation="MGRRAVIVVLDGVGAGDAPDAVEFGDEGANTLGNTARAVGGLDL
                     PNLQNLGLGNVAEIEGVPAEESPRASHGLMVELSAAKATLAGHWEMMGLILEDPLPTY
                     PEGFPEEIIHR"
     gene            524..1003
                     /locus_tag="VFT03_01095"
     CDS             524..1003
                     /locus_tag="VFT03_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4846765.1"
                     /translation="MAELRVREASAEDAEIIHSLAAELAGAVGDSPPSLEDVRERLAE
                     LLEERRARVLVAEGDVVVGVVSLWIKPDLAHGDSVVEVPMLVISEDRRREGVGKLMME
                     EVQRLAAENGATLIELVATTQNNPAREFYRSLGFVETDHVTLEFVGDVEDPPDPEET"
     gene            complement(1000..1536)
                     /locus_tag="VFT03_01100"
     CDS             complement(1000..1536)
                     /locus_tag="VFT03_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846766.1"
                     /translation="MAKRGYTKDTIRGGASDWDVSRPFSSYLRMLGSLLTHPVRFFEV
                     LPRVSDVRAPGLFLVFSGLPAAALWLTFGGPYPALATLLLPLPLSFPISALYHLASFG
                     GRYGYLVTWRIVAYPFGFYLPLSAVPVVRWVAAAYAGVVLVGAGLAVVREIGAIRAAL
                     ASVAVTGSLVLAAYLLAS"
     gene            1631..>2260
                     /locus_tag="VFT03_01105"
     CDS             1631..>2260
                     /locus_tag="VFT03_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU4846767.1"
                     /translation="MPENNAFDAQTGLGTGTGEVTIHSLKRLDERVDGDVFSLPFSVR
                     VILESLLRNCGGKFVSEEDVEALASWPGSVGEELAYLPSRVVMQDFTGVPAVVDLAAM
                     REAMIKLGGKAQQINPLAPAELVIDHSVQVDEYGAPDSLLRNNKIEFERNGERYAFLR
                     WGQTAFSNFSVVPPNTGIVHQVNLEHLARVVFDSERDGVRWAYPDTLVGT"
BASE COUNT          428 a          717 c          760 g          355 t
ORIGIN      
        1 acggtggatg atctcctccg ggaagccttc gggataggtg ggcaacgggt cttccaggat
       61 cagtcccatc atctcccagt gcccggctag cgtggccttg gccgcggaga gctcgaccat
      121 gagcccgtgc gaggcgcgtg gggactcttc ggccggtacg ccctctatct ccgccacgtt
      181 gccgaggccg aggttctgga ggttcgggag atccaggccc cctacggccc gcgccgtgtt
      241 gcccagggta ttggccccct cgtccccgaa ctcgacggcg tccggcgcgt cgcccgcgcc
      301 cacgccgtcg aggaccacga ttacggccct gcgtcccatg ctcctccttt tacaaccggc
      361 atctagcagg gctgccggag catctcccta ccctttcctc gcccgttttt gtgtcctggg
      421 acgcaaaaaa cgtatccctt tgacccattc tggcaggacc ggatgtaccg ggccaccatc
      481 gttgcggtag agtgactctg ccggtgtaga gagaaaggac accttggccg agctgcgcgt
      541 gcgggaagcg agcgccgaag acgctgagat cattcactcc ctggctgcag agctagccgg
      601 ggccgtgggc gactcacccc caagcctgga ggacgtcagg gagcgactcg ccgagctgct
      661 ggaggagcgg cgggcgcggg ttctggtggc ggagggcgac gtagtcgtcg gggtggtgag
      721 cctctggatc aaacccgacc tcgcccacgg cgacagcgtc gtcgaagttc ctatgctcgt
      781 catctccgag gaccggcgca gggagggagt aggcaagctc atgatggagg aggtgcagcg
      841 tcttgccgcc gagaacgggg ccaccctcat tgagctagtc gccacgaccc agaacaaccc
      901 ggcgcgcgag ttctaccgct ctctggggtt cgtcgagacc gaccacgtca ccctggagtt
      961 cgtcggcgac gttgaggacc cgccggaccc cgaagagacc tagcttgcca gcaagtacgc
     1021 cgcgagcacg agagatccgg ttaccgcgac cgaggcgagc gcggctctga tcgccccgat
     1081 ctcgcgcacc accgcgagcc ctgctccgac cagtactacc cccgcgtacg ccgcggcgac
     1141 ccagcgcacc acgggcaccg cagagagagg caggtagaag ccgaaggggt aggcgacgat
     1201 ccgccaggtc accaggtagc cgtacctgcc gccgaaggag gcgaggtggt agagagcgga
     1261 gatggggaag gagagcggga gcggcaggag aagggtggcg agcgcggggt agggaccccc
     1321 gaaggtgagc cacagagcag ccgccggaag ccccgagaag acgaggaaga gacccggagc
     1381 ccgcacgtcc gatactctag gcaagacctc gaagaagcgg acaggatgag tgaggagact
     1441 ccctagcatc ctcaggtagc tcgagaaagg ccgcgagacg tcccagtcgc tcgcgccgcc
     1501 ccggatcgtg tccttcgtat aaccgcgctt ggccaaacta cagccagtat ataagcgtct
     1561 acgacggaaa gcggcgcgag atcatctata atctgagtgg atcaagcata gagaagaggt
     1621 tcgcatctac atgccagaaa acaacgcttt cgacgcgcag acagggctcg gcaccggcac
     1681 tggtgaggtg actatccaca gcctgaagag gctggacgaa agggtggatg gggacgtctt
     1741 ctcgctgcct ttctcggtgc gcgtgatcct cgagtcgctc ctgcggaact gcggcggcaa
     1801 gttcgtctcc gaggaggacg tcgaggctct ggcctcctgg cccgggtcgg tgggggagga
     1861 gctggcttac ctgccgtcgc gcgtggtgat gcaggacttc accggggtgc cggcggtcgt
     1921 cgacctcgcc gcgatgcgcg aggccatgat caagctcggc ggcaaggcgc agcagatcaa
     1981 cccgctggca ccggcggagc tcgtaatcga ccactccgtg caggtcgacg agtacggtgc
     2041 gcccgactcg ctgctccgca acaacaagat cgagttcgag cgcaacggcg agcgctatgc
     2101 gttcctgcgc tggggccaga ctgcgttcag caatttcagc gtcgtgccgc cgaataccgg
     2161 catcgtgcac caggtcaacc tcgagcatct cgcccgcgtc gtgttcgaca gcgagagaga
     2221 cggcgtgcgc tgggcctatc cggacacgct ggtcggcacc
//
LOCUS       DASTUV010000051         5023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_923090, whole genome shotgun sequence.
ACCESSION   DASTUV010000051 DASTUV010000000
VERSION     DASTUV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923090"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1930)
                     /locus_tag="VFT03_01110"
     CDS             complement(308..1930)
                     /locus_tag="VFT03_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEU4846768.1"
                     /translation="MSDQAISITFFVLIIALTLGITVWASRRNVDTSHHYVAGGEIKG
                     WQNGLAISGDYLSAASFLGIAGAIALTGFSGFYLSIGFLVAYLVVLLLVAEPLRNLGK
                     YTMADMLASRFNVSSVRSAAALSTITISMFYMIAQLNGAGALIGLLLGFPYWVSVIGI
                     GILMTIYIVAGGMIATTWIQIIKAVLLIAGTLLLSILVLAKVGFNPLALFSEVQSEFG
                     KEMLAPPPPSGLVAGLDVISLNIALVLGTAGLPHILIRFLTVPDAKTARSSIVTATWI
                     IGLFYLLTPVLGYGSALFVGQDAISEANVAGNLAAPQLAEQLGGPVFFAFISAVAFAT
                     IVAVIAGLVVAASSAFAHDFYSNVIRHGQATEREQFTAARITAIGISVAAIILAIPLG
                     SLNVAFLVALAFAVAASANVPVILLTIFWSRFNTMGAIIGMLVGLISAVVLVIISPNV
                     FTGPNPEPPATPIITIDPIFPLKSPALISVPLGFLGCYLGTLLGGRRDAREREQGIQR
                     SYDEIRVRALTGISDIEREIKEAAPKKEPRTT"
     gene            complement(1932..2246)
                     /locus_tag="VFT03_01115"
     CDS             complement(1932..2246)
                     /locus_tag="VFT03_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEU4846769.1"
                     /translation="MDRDAEWLRIERTSAFKELVRKRKSFIIPATIFFFVFYFGLPVL
                     AGFTTVLDGYAIGAITWAYVYAFAQFAMTWILMHMYVSRANKWDELVERARREAAEGD
                     AS"
     gene            complement(2405..2890)
                     /locus_tag="VFT03_01120"
     CDS             complement(2405..2890)
                     /locus_tag="VFT03_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4846770.1"
                     /translation="MATLEPLRFRRIMVPVDESKPSQNSLAVAAGLARETGAQVILTH
                     VVQMRWSQDEPEMRATYGEIVDDYRTSGEETLRRTASSETFSGLDVDTQLLFGNNPAR
                     ELLRLAKEREVDAIVMGSHGRGRFGGMMLGSVSQRVIHDATVPVIIVPPHLERAEEAS
                     G"
     gene            complement(2938..5016)
                     /locus_tag="VFT03_01125"
     CDS             complement(2938..5016)
                     /locus_tag="VFT03_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4690..4692),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU4846771.1"
                     /translation="MQKVTGEKANPSSKGEACIKGREGWRHLYSDRRLTTPLIRKDGE
                     LVQASWDEALDLVASKMTEIKGEHGPGAFGLFSSSRSSNELNYLAGKFVRQVLGNSNI
                     DSCNRAUHAASVTGLVATFGAGGATTSYEELENTDLIICWGSNTMECHPIIYNHMRRG
                     IKNGAKMVVIDPRKIDQVKKADKWLGLNVGTDICLANAMGHVIIEEDLYDKEFVEKAT
                     EGFEAYTEKVAGYTPEYAEQVTGVPAGDIREVARMYATANKATLNWTLGITEHHNGAD
                     AVFALIGLGLLTGHVGKYGSGLNPLRGQNNVQGGGDMGALPDKMVGGWKWDDPEAHEL
                     FEDVWGSPLPEKVGKNQAEMMDAVANGEIRCMYVIGENPAQSDADEHKHEKLFESLDF
                     LVVQDILMTKTARMADVVLPAAASWAETDGTVINSERRVQRIRRAVDPPGQARDDVMI
                     LRDIANRMGQVWSFDTAEEVWDEVRRLAPKFSGISYERLEKYGGIQWPCFDGSFEEIS
                     DITPKEQLAASTRGAENAGGRAITTDVMEKPQVGTKFLHAELRADEVENRVPFTPVDH
                     EGPIEKPDDEYPFQLTTGRRLAFHNTGTMTQDYKKVKDNEELLEICAEDAEEYGIEDG
                     DMVRISSRRGTVEAVKARVTDRVRPGLLFMGYSFPDQVPTNVLTINAVDPQSGTAELK
                     ACAVKVEPAA"
BASE COUNT          847 a         1628 c         1555 g          993 t
ORIGIN      
        1 tttccgaaga accactcctc ccttactccc tggggcgggg gaagatgcgc gctaatccct
       61 cgcccatact acgtagtaca cgtaagaata caacgctcgt ggggttccac acatcactca
      121 aatgagtgat ctttactatt acttgggtgt tactaaatgt agcgaagaca tcaccacgcc
      181 cctatcgttg acgtcctcca ggacacgggg acagtggagc gcagatcgga acaccccaac
      241 cgaagacgag cccccgccgg tggacggggg cttcggtcta cgtggtgcgt ctttatgtgg
      301 tgcgtcttta cgtggttcgc ggctcctttt tcggggcggc ctctttgatc tcccgttcga
      361 tgtcgctgat gccggtgagg gcgcggacgc ggatctcgtc gtaggacctc tggatgccct
      421 gctcgcgctc gcgcgcgtcc ctgcggccgc ccaggagcgt gccgaggtag cagccgagga
      481 agccaagcgg cacggagatg agggcagggc tcttcagcgg gaagatcggg tctatggtga
      541 tgatcggggt cgccggcggc tcggggttcg gtccggtgaa cacgttgggg ctgatgatca
      601 cgagcacgac ggcggagatc aggccgacaa gcatccctat gatcgccccc atagtgttga
      661 acctgctcca gaagatagtg agcaggatga ccggcacgtt ggcgctagcg gcgaccgcga
      721 aggcgagcgc cacgaggaac gccacgttga ggcttccaag ggggatggcc aggataatcg
      781 cggcgaccga gatgccgatg gccgttatgc gggcggccgt gaactgctcg cgctcggtgg
      841 cctgcccgtg acgtatcacg ttactgtaga agtcgtgggc gaaggcgctc gaggccgcga
      901 ccacgagccc cgcgatcacc gccacgatcg tcgcgaaggc tacagccgag ataaacgcga
      961 agaacacggg ccccccgagc tgctcggcga gctgcggagc ggccaggttg cccgccacgt
     1021 ttgcctccga gatggcgtcc tgcccgacga agagcgccga gccgtagccg aggaccgggg
     1081 taagcaggta gaacaggccg atgatccagg tcgccgtgac gatggaggac ctggcggtct
     1141 tcgcgtcggg cacggtgagg aaccggatca ggatgtgcgg cagacccgcc gtcccgagga
     1201 cgagggcgat gttgagcgag atcacgtcga gcccggcgac cagaccgctc ggaggaggcg
     1261 gagccagcat ctctttgccg aactcactct gcacctcgga gaacagcgcg agcgggttga
     1321 agcccacctt ggccagcaca aggatgctca ggagcagggt gcccgcgatg agcagcacgg
     1381 ccttgatgat ctggatccag gtcgtcgcga tcatgccccc ggcgacgatg tagatggtca
     1441 tgagtattcc gatccctatg accgataccc agtatgggaa gccgagcaac agcccgatga
     1501 gcgcccccgc cccgttgagc tgggcgatca tgtagaacat gctaatggtg atcgtgctca
     1561 aagccgccgc agagcggacg ctgctcacgt tgaagcggct cgcaagcatg tccgccatcg
     1621 tgtacttgcc caggttcctc aacggctcag ctaccagtag caacaccacc aggtaagcga
     1681 cgaggaagcc tatcgagagg tagaaacccg agaacccggt cagggcgatg gcgcctgcaa
     1741 tgccgaggaa cgaggccgcg gacaggtagt cgcccgagat ggcgagcccg ttctgccagc
     1801 ccttgatctc gcctccggcc acgtagtggt ggctcgtgtc gacgttccgc ctcgacgccc
     1861 agaccgtgat gccgagcgtg agcgcaatga tgaggacgaa gaatgtgatg gagatggctt
     1921 gatcgctcac tttagctggc atccccctcc gcggcctcgc gccgcgcccg ttccacgagc
     1981 tcgtcccact tgttagcccg gctgacgtac atatgcatga gtatccaggt catcgcgaac
     2041 tgggcgaacg cgtagacgta cgcccacgtg atcgcgccta tagcgtaccc gtccagaacg
     2101 gtcgtgaacc ccgccaggac cggcagcccg aagtagaaca cgaagaagaa gatcgtcgcc
     2161 ggtatgatga acgacttcct cttccgtacc agctccttga atgcagacgt acgctcaatg
     2221 cgcagccact ccgcgtctct atccacccgt tctcctttct cgttccccct gccaaacccg
     2281 gcaccactct ggacgtaagt aaggcggtca accggataca tacccgtcac tgaactcccg
     2341 aaacgcgcct acccggggac ctcgtcgggc cagatctcga gctcctgctg ggttccgcgc
     2401 agcgctaccc gctggcttcc tccgcgcgtt cgaggtgggg cgggacgatg atcacgggta
     2461 ccgtggcgtc gtggatgacg cgctggctaa ccgaaccaag catcatccct ccgaaccgcc
     2521 cgcgtccgtg gctgcccatg actatggcgt ctacctcgcg ctcttttgcg agccgcagca
     2581 gctctcttgc cgggttgttt ccgaagagca gctgcgtgtc cacgtcgagc cctgagaagg
     2641 tctcgctgct tgcggtgcgc ctcagcgtct cctcgccgga tgtgcggtag tcgtccacga
     2701 tctcgccgta ggtcgccctc atctccggct cgtcctggga ccagcgcatc tggaccacgt
     2761 gcgtcaggat cacctgcgcc cccgtctccc gcgccagccc cgccgcgacc gccagcgagt
     2821 tctgcgacgg cttcgactcg tcgacgggga ccatgatccg cctgaacctc agcggctcca
     2881 gcgtcgccat ttcaccacct cctgcgtcgt atccacgtgc accgggcgtg tatcttgcta
     2941 cgcagcgggc tctaccttca ccgcgcaggc cttgagctcg gcggtccccg actgggggtc
     3001 cacggcattg atggtcagga cgttggtggg tacctgatcc gggaagctgt aacccatgaa
     3061 cagcagtccc ggtctcaccc tgtcggtgac gcgggccttg accgcctcga cggttccacg
     3121 gcgggaacta atcctgacca tgtcgccgtc ctcgatgccg tactcttcgg cgtcttcggc
     3181 gcagatctcg agcagttcct cgttgtcttt gacctttttg tagtcctggg tcatcgtgcc
     3241 cgtgttgtgg aatgcgaggc ggcgcccggt tgtcaactgg aacgggtact cgtcgtcggg
     3301 cttctcgatc ggcccctcgt ggtccacggg ggtgaacgga acgcggttct cgacctcgtc
     3361 ggcccgcagc tcggcgtgca ggaacttggt tcccacctgc ggcttctcca tcacgtcggt
     3421 ggtgatggct ctaccgccgg cgttctcggc gcctctggtg ctggcggcta gctgttcttt
     3481 gggggtgatg tcggagatct cctcgaagct cccatcgaag cacggccact ggatgccgcc
     3541 gtacttctcc agccgctcgt agctgatgcc gctgaacttg ggcgcgagac ggcgcacctc
     3601 gtcccagact tcctcagcgg tatcgaagct ccaaacctgg cccatacggt tggcgatgtc
     3661 gcgcaggatc atcacgtcgt cgcgcgcctg gccgggcggg tccactgctc tgcgtatccg
     3721 ctgcacccgc cgctcgctgt tgatgaccgt gccgtcggtc tcggcccagc ttgcggccgc
     3781 gggcagcacc acgtccgcca tcctggcggt cttggtcatg aggatgtctt gaaccacaag
     3841 gaagtccagg ctctcgaaga gcttctcgtg cttgtgctca tcggcgtcgg actgcgccgg
     3901 gttctccccg attacgtaca tgcaccggat ctcgccgttc gcaacggcgt ccatcatctc
     3961 ggcctgattc ttgccgacct tctctggcag cggcgagccc cacacatctt cgaacagctc
     4021 gtgcgcttcg gggtcgtccc acttccagcc gccgaccatc ttgtcgggca gggcgcccat
     4081 gtcgccgccg ccctgcacgt tgttctggcc gcgcaacggg ttcaggcccg agccgtactt
     4141 gccgacgtgg ccggtaagga gccctaggcc gatgagcgcg aagaccgcgt ccgcgccgtt
     4201 gtggtgctcg gtaatgccga gcgtccagtt cagggtagcc ttattggcgg tggcgtacat
     4261 gcgagctacc tcgcgaatgt ccccggccgg gacgcccgtc acctgctcgg cgtactcggg
     4321 ggtgtagccc gccaccttct ctgtgtacgc ctcgaagcct tcggtcgcct tctcgacgaa
     4381 ctccttgtcg tagaggtctt cctcgatgat gacgtggccc atcgcgttag ccaggcagat
     4441 gtcggtaccg acgttgaggc cgagccactt gtctgccttt ttgacctgat ctatcttgcg
     4501 cgggtctatg acgaccatct tcgcgccgtt ctttatgccg cggcgcatgt ggttgtagat
     4561 gatggggtgg cactccatgg tgttcgatcc ccagcagatt attaagtctg tgttctccaa
     4621 ctcctcgtag gaggtagttg ccccgcccgc tccaaacgtt gccaccagac cggtgacgct
     4681 ggcggcgtgt caagcacggt tacatgagtc tatgttgctg ttcccgagca cctgccgcac
     4741 gaacttcccg gccaggtagt tgagctcgtt ggaagagcgg gaggagctaa acagcccgaa
     4801 ggcgccggga ccgtgttcgc ccttgatctc ggtcatcttg ctcgcgacga gatccagggc
     4861 ctcgtcccac gaagcctgga cgagttcgcc gtccttgcgg atgaggggcg tagtcagtct
     4921 cctgtcgctg tagaggtggc gccagccctc gcggcctttg atgcacgcct cgcccttgct
     4981 cgacgggttt gccttctcgc ccgttacctt ctggatgacc ccg
//
LOCUS       DASTUV010000052         7410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_422134, whole genome shotgun sequence.
ACCESSION   DASTUV010000052 DASTUV010000000
VERSION     DASTUV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422134"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /gene="radC"
                     /locus_tag="VFT03_01130"
     CDS             <1..452
                     /gene="radC"
                     /locus_tag="VFT03_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU4846772.1"
                     /translation="AVEFGKRLGRVRNPGRSVVSSPADVDGLLRGRIANLDRENFVAV
                     LLNTKNEVIGSPTISVGTLSASLVHPREVFKPAIRASAASVVLAHNHPSGRVEPSSED
                     REVTARLVAAAEIIGIEVLDHVILGDGYFSMKEHGMLQARSALGPRT"
     gene            472..1500
                     /locus_tag="VFT03_01135"
     CDS             472..1500
                     /locus_tag="VFT03_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU4846773.1"
                     /translation="MFGGLFGRDVAIDLGTANTLVFVKGHGIVLSEPSVVAIDNKTDK
                     VVAVGSAAKRMLGRTPGSIVAMRPLKDGVIADFEVTEKMLSYFIRKVQPQRRLFRSLI
                     GPRVVVCVPSGVTGVELRAVKEATESAGARQAYTIEEPLAAAIGAGLPVNEAQGSMII
                     DIGGGTSEVAVISLGGIVTKSSIRIAGDDIDDAISTYIQKEYKLAVGTQTTEQLKIEL
                     GSAFRLEEEESAEIRGRDLVTGLPKTIVITSEEVREAISVPVEAIIAAVRDTLDRTPP
                     ELASDIMDRGMVLVGGGAMLRHLDERLRRETGIPVHVADDALMCVAIGSGRSLEEIDS
                     YRGALYSD"
     gene            1506..2447
                     /gene="mreC"
                     /locus_tag="VFT03_01140"
     CDS             1506..2447
                     /gene="mreC"
                     /locus_tag="VFT03_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU4846774.1"
                     /translation="MGRRKSSAAGGLTALFILCVASLALFTVYVKEADCTKAGKCGPL
                     HTVQLGAAEVLRPVRDAVAMIVEPFQEVGERVTGAFDNSDEEAIEAKFRQTQGLAAQA
                     SRLERENARLRELLKGERSFYEYGPLARVVAPVSDQFTNRIQIDVGSEDGIKPQMPVV
                     VGENTLVGRTTNDVSRSTAQVMLVTDREFAAGVRIIPPAQFNPASGQLSPAVTDPDVS
                     YGEGLLRTNLEGYFGIEYVDLSSRAEKGDYVVTSGRAGERELLFPPGLFVGTIESVSS
                     QDIDQYKKIVVEPAMDPDDLEEVRVITDWGPGPGVPG"
     gene            2448..2963
                     /gene="mreD"
                     /locus_tag="VFT03_01145"
     CDS             2448..2963
                     /gene="mreD"
                     /locus_tag="VFT03_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU4846775.1"
                     /translation="MEQPSVVRASLLVAAAALLDAVLGPYLTFGWISPKLMVLGVVFA
                     VAPLRDLQAVLLGFFGGVLFDALSGGLFGVGALSGLLAGILSARAGDVRPKGAERLLL
                     AQVVAISVAAYDLTYLTALNFAGLAAPPFGQYAVAGVIPDALLNAVLAYFVGGWWLRL
                     GRDKTDAWDER"
     gene            2960..4099
                     /gene="rodA"
                     /locus_tag="VFT03_01150"
     CDS             2960..4099
                     /gene="rodA"
                     /locus_tag="VFT03_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU4846776.1"
                     /translation="MTLTRKEAKTDKTANLPKWRSLDPVLLLVSLALSAFGILAVYVA
                     STVYQQANATNQALGFVLGMVGAVPLALIDYRLWQRYLRPIYGLVILMLLSVLLLGAA
                     AGGAQRWVDIGPVQVQPSEFAKLGMVVVLAGYFAERAVGEYTVFFKALGVLSVPALLV
                     FMQPDLGTALVFGAVFVAMAYIAGARMVQLAGLVAAAVAAAVLAVKLNILEDYQVARL
                     TAFMKPDGTSDVGYQVAQSKMAIGSGGITGKGLDAQTLANLGFLPEDHTDFIFSNLAE
                     RVGFVGSIVLIALFFLLVWRILHVATISRDRFGVLIAVGVATIFLFHVFVNVGMTMGI
                     MPVTGIPLPFISYGRSNLVVSVLSLGLLQSIAIHSKAEASKQPKV"
     gene            4205..4504
                     /gene="rplU"
                     /locus_tag="VFT03_01155"
     CDS             4205..4504
                     /gene="rplU"
                     /locus_tag="VFT03_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU4846777.1"
                     /translation="MIAVLKSGGKQYRVTEGQELVVDRLGGEVGDSLELPVGFVADAE
                     SVDLGQRTARVEILEHLRGDKIHVYKYKPKKDYRKKTGHRQELTRIRILEVSDGT"
     gene            4494..4769
                     /gene="rpmA"
                     /locus_tag="VFT03_01160"
     CDS             4494..4769
                     /gene="rpmA"
                     /locus_tag="VFT03_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU4846778.1"
                     /translation="MAHKKGGGSSRNGRDSAGKRLGVKTYGGQLVSAGSIIVRQRGTK
                     VHPGANVGKGSDDTLFAKVGGRVSFDRSRGRSVVSVLEESLLEEATA"
     gene            4801..6039
                     /gene="obgE"
                     /locus_tag="VFT03_01165"
     CDS             4801..6039
                     /gene="obgE"
                     /locus_tag="VFT03_01165"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU4846779.1"
                     /translation="MQFVDEARFVVRAGSGGDGSVSFNREKYKPRGGPDGGRGGDGGS
                     VVLRATEDLSTLEPFANRRVVKAERGGHGSGNNRAGERGKDAILEVPVGTLIHDESGQ
                     LADLSEAGASFVVARGGEGGKGNASFATSTRQTPAFREKGLPGEEREIRLELRVLSDV
                     GLVGLPNAGKSSLLRALSAARPRVGDYPFTTLSPQLGVADDRRYREPFVVADIPGLIS
                     GASQGRGLGNRFLRHVARARLLALILDASEDPEGADAALRAELHAAGLSRKPTLTVLN
                     KVDLLDEELREYLREAFPDAIQVSAKTGEGVEELRDLLEERLRVLDDRQEEQPERREH
                     RIFRPKWKGVRVQRENGGYVVAGEGVERLALKTDWENSEGVERFQRELERSGVVSALR
                     RAGAQPGDEVRIGETVFDFR"
     gene            6050..6697
                     /gene="nadD"
                     /locus_tag="VFT03_01170"
     CDS             6050..6697
                     /gene="nadD"
                     /locus_tag="VFT03_01170"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU4846780.1"
                     /translation="MKAGIFGGTFDPIHQGHMVIAEQVMGELGLDHILFVPAGIPPHK
                     EASSVRARTEDRYEMVEAAVEGNERFSVDRVEVDAGRPMHSVETVQILKERSPDDEWF
                     FITGADEVSNLLAWKDPDRLLEEVVMVAATRPGYDLSRLDHLEAKLRNFDRIFPVECT
                     RVDISATGIRRRILQGKSVRYLVPEEVYEIIVERGLYEGDEKRSGQGGRLKEETR"
     gene            6694..7092
                     /gene="rsfS"
                     /locus_tag="VFT03_01175"
     CDS             6694..7092
                     /gene="rsfS"
                     /locus_tag="VFT03_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU4846781.1"
                     /translation="MSRDESAIRRESLETDGEALSYEMATTAARAASEMFARDVTIVD
                     LRDLVSYTDYFVIASAETERQTRRVAQEVIQRMIEAGHRPRTKRIEEGTTWISLDFLD
                     VVVHVLTDEARDYYRLESFWRSAPQEHWEE"
     gene            7170..7242
                     /locus_tag="VFT03_01180"
     tRNA            7170..7242
                     /locus_tag="VFT03_01180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7203..7205,aa:Ala,seq:ggc)
BASE COUNT         1343 a         2276 c         2518 g         1273 t
ORIGIN      
        1 ctgctgtgga gttcgggaag agattggggc gggttcgcaa ccccggacgc tccgtggtat
       61 catcccccgc ggacgtggat gggctcttga gggggcggat cgccaatctt gatcgggaga
      121 acttcgtcgc cgtgctcttg aacacgaaaa acgaagtcat agggtctccg acgatctcgg
      181 tcggcacgct ctcagcctcg cttgtccacc ccagggaggt tttcaaaccg gccatccggg
      241 cgagcgcggc aagcgtcgtg ctcgcgcaca accaccctag cggcagagtc gaaccgagca
      301 gcgaggaccg cgaggtcacg gccaggctgg tggccgcggc ggaaattatc ggcatagagg
      361 tgctcgacca cgtgatcttg ggcgacgggt actttagtat gaaggagcac gggatgctcc
      421 aggcgcgaag cgcgctgggg ccgagaactt gagcagcagg atggggcgct tatgtttgga
      481 ggactgttcg gacgggacgt tgcgatagac ctgggtacgg cgaacacgct ggtgttcgtc
      541 aaggggcacg ggatcgtgct ctccgagcct tcggtagtcg ccatagataa caagaccgac
      601 aaggtcgttg cggtggggtc cgcggccaag agaatgctcg gccgcacgcc tggcagcatc
      661 gtggccatgc ggccgctcaa ggacggcgtg atcgcagact tcgaggtcac cgagaagatg
      721 ctctcctact tcatccgcaa agtgcagccg cagaggcgcc tgttcaggtc cctgatcggg
      781 cctcgcgtcg tggtctgcgt gccttcgggg gtgaccggcg tcgagcttcg ggccgtcaag
      841 gaggcgaccg agtcggccgg agcgcgccag gcctacacca tcgaggagcc tctggctgcg
      901 gcgataggcg cggggctgcc ggtcaacgag gcgcaggggt ccatgatcat agacatcggc
      961 ggcggcacga gcgaggtcgc tgtgatctcc cttggcggca tcgtcacgaa gtcctctatc
     1021 cgcatagccg gcgacgacat cgacgacgcc atcagcacct acatccagaa ggagtacaag
     1081 ctggcggtgg gcacgcagac gaccgagcag ctcaagatag agctcggcag cgccttcagg
     1141 ctcgaggagg aggagtctgc ggagatccgg ggtcgcgacc tggtgaccgg gcttcccaag
     1201 accatcgtca taaccagcga ggaggtccgc gaggccataa gcgtgccggt cgaggcgatc
     1261 atcgctgccg tacgggacac gctcgaccgc accccgcccg agctcgcctc ggacatcatg
     1321 gaccgtggga tggtgctcgt gggaggtgga gcgatgctca ggcacctcga tgagcgcttg
     1381 agacgcgaga ccggcatccc cgtgcacgtc gccgacgacg ctcttatgtg cgtggccatc
     1441 ggcagcggcc gctccttgga ggagatagac tcctaccgcg gcgccctcta ctcagactag
     1501 gcgtagtggg acggaggaag tcaagcgcag caggaggtct gacggcccta ttcatcctgt
     1561 gcgttgcgag cctcgcgctc ttcaccgttt acgtgaagga ggcggattgc accaaggcgg
     1621 gcaagtgcgg gcctctgcac acggtccagc tcggcgccgc cgaggtcctc agacccgtgc
     1681 gtgacgcggt cgccatgatc gtcgaacctt tccaggaggt gggggagagg gtcactggcg
     1741 ccttcgacaa cagcgatgag gaggcgatcg aggcgaagtt ccggcagact caggggctcg
     1801 cggcccaggc atcacgcctg gagcgggaga acgccaggct gcgcgagctc ctcaaaggtg
     1861 agcggtcctt ctacgagtac gggcctctgg ccagggtggt cgcgcctgtg agcgaccagt
     1921 tcaccaacag gatccagata gacgtcggct ccgaggacgg gataaagccc cagatgcccg
     1981 tcgtcgtcgg cgagaacacg ctcgtggggc gcaccaccaa tgatgtcagc cgcagcacgg
     2041 cccaggtcat gctcgtcacc gaccgggagt tcgccgcggg cgtcaggatc atcccgccgg
     2101 cccagttcaa tcccgcctcg ggccagttga gccctgccgt cacggacccc gatgtctcct
     2161 acggcgaggg gctcctcagg accaaccttg aggggtactt cgggatcgag tacgtcgacc
     2221 tgagctcgcg ggctgagaag ggcgactacg tggtgaccag cgggcgtgcg ggggagaggg
     2281 agctcctgtt cccgccgggt ttgttcgtgg ggactatcga gtccgtaagc tcgcaggaca
     2341 tagaccagta caagaagata gtcgtcgagc cggcgatgga ccccgacgac ctcgaagagg
     2401 tgcgtgtgat cacagactgg ggtccgggcc ccggggttcc tggatagatg gaacagccct
     2461 ccgtcgtccg cgccagcctc ctcgtcgccg cagccgcgct cctcgacgct gtcctcggcc
     2521 catacctgac ctttggctgg atctcgccta agctgatggt gttaggcgtc gtcttcgcgg
     2581 tcgcgccgct gcgggacctg caggccgttc tgctcgggtt cttcggcggc gtactctttg
     2641 acgccctgag tggtggtctc ttcggagtgg gcgctttaag cggtctgctc gcggggatcc
     2701 tctcagcgcg cgccggggac gtccggccca agggcgccga gaggctgctg ctggcccagg
     2761 tcgtcgcgat ctcggtcgcg gcctacgacc tgacctacct tacggcgctc aacttcgccg
     2821 ggctcgcggc ccccccgttc ggccagtacg ccgtcgcggg cgtgatcccc gacgccctgc
     2881 ttaacgctgt gctcgcctac ttcgtgggcg ggtggtggtt gcgccttggc cgcgacaaga
     2941 ccgacgcctg ggacgagaga tgaccctcac tcgcaaggag gcgaagaccg acaagaccgc
     3001 caacctgccg aaatggaggt cccttgatcc ggttctactc ctggtctccc tggcgctctc
     3061 cgccttcggc atcctcgccg tctacgtggc cagcacggtt taccagcagg ccaacgctac
     3121 caaccaggcg cttggtttcg tcctcgggat ggtaggggcc gttccactgg cgctcataga
     3181 ctaccggctc tggcagcgct acctcaggcc catctacggc ctcgttatcc tcatgctcct
     3241 ctccgtgctg ttgcttggag ctgcggccgg cggcgcccag cggtgggtgg acataggacc
     3301 cgtgcaggtg cagccttccg agttcgccaa gctgggcatg gtcgtagttc tggccgggta
     3361 tttcgccgag agggccgtcg gcgaatacac cgtcttcttc aaagccctcg gcgtactctc
     3421 cgtccccgcg ctcctggtct tcatgcagcc ggacctcggt accgcgctgg tcttcggagc
     3481 ggtcttcgtc gccatggcgt acatcgccgg ggcgcggatg gtccagctcg caggcctcgt
     3541 cgccgcggcg gtcgccgccg cggtccttgc ggtcaagttg aatatcttgg aggactacca
     3601 ggtcgcccgc ctgacggcct tcatgaagcc ggacgggacc tcagacgtcg gttaccaggt
     3661 ggcgcagtcc aagatggcta tagggtcggg tggtattaca ggcaagggac tagacgccca
     3721 gacgctcgcc aacctcggct tcctgcccga ggaccacacg gacttcatct tctccaacct
     3781 cgccgagagg gtgggcttcg ttggcagcat agtgctcatc gcgctgttct tcttgctcgt
     3841 ctggcgcatc ctccacgtcg cgaccatctc ccgcgaccgt ttcggggttt tgatcgccgt
     3901 aggcgttgca accatcttcc tcttccacgt gttcgtaaac gtcgggatga cgatggggat
     3961 catgcccgtg accggcatcc cgctgccgtt catcagctac ggacggagta acctggtggt
     4021 cagcgtcctc tctctgggcc tcctgcagag tatcgccatc cactccaagg ccgaggcctc
     4081 caagcaaccg aaggtgtagc gatctactgg tcgatacgcg agatggcggt tatactgtcg
     4141 ttccgaatag tacgttttgt acgcctggag atcagagtct actaaacctc gggagcctat
     4201 aatcttgatt gcagtactaa agagcggtgg aaagcagtac agggtgacgg agggccagga
     4261 gctggtagtc gacaggcttg gcggcgaggt cggagactcg ctcgagctgc cggtcggctt
     4321 cgtggccgac gcggagagcg tcgacctcgg ccagaggacg gctagagttg agatactcga
     4381 gcatctgcgg ggagacaaga tccacgtcta caaatacaag ccgaagaagg actacaggaa
     4441 gaagaccggg cacaggcagg agctgacccg gatccggatt ttggaggtat ctgatggcac
     4501 ataagaaagg cggcggctcg tcgcgcaacg gccgcgattc cgctggcaag cggctcggcg
     4561 tgaagaccta cgggggccag ctggtgagcg ccggcagcat catcgtgcgc cagcgcggca
     4621 cgaaggtgca ccctggcgcg aacgtcggca agggctccga cgacacccta ttcgcaaaag
     4681 taggcggcag ggtaagcttc gatcgcagcc gcggccgcag cgtggtcagc gtactggagg
     4741 agtcgctcct tgaggaggcc acggcctgag gaataggcgt cccgggggta tctagccccg
     4801 ttgcagttcg tagatgaagc ccggttcgtc gttcgcgcag gtagcggcgg cgacggcagc
     4861 gtctctttta accgcgagaa gtacaaaccg cgcggcggac ctgacggcgg ccgcggcggc
     4921 gacggcggct ccgtcgtcct gcgcgccacc gaggacctct ccaccctcga acccttcgcg
     4981 aaccgcaggg tagtgaaagc ggagcgcggc gggcacggct cgggtaacaa ccgcgccggc
     5041 gagcgcggga aagacgcaat cctcgaggtg cctgtcggca ccctgatcca cgacgagagc
     5101 ggccagctcg ccgacctctc cgaggccgga gcatccttcg tcgtcgcccg cggcggcgag
     5161 ggcggcaagg ggaacgcctc gtttgcgacc tcgacccgcc agacgcccgc gttccgcgag
     5221 aagggattgc cgggggagga gcgtgagatc cggctcgagc tcagggtcct ctctgatgtt
     5281 ggcctcgtcg ggttgcccaa cgcaggcaag tcctcgctgc tgcgagctct aagcgccgcc
     5341 cgtccgcggg taggcgacta cccttttacg acgctctctc cacagctcgg ggtggcggac
     5401 gacaggcgct accgcgagcc cttcgtggtc gcggacattc cgggcctgat ctcgggcgcg
     5461 agccagggcc ggggcctcgg caaccgtttc ctcaggcacg tcgcgcgggc gcggctcctg
     5521 gccctcatcc tcgacgcgag cgaggatccg gaaggagcag acgccgctct gcgagccgag
     5581 ctgcacgcgg ctggcctctc caggaagccg acgctcacgg tgctcaacaa ggtggaccta
     5641 ctggacgagg agctccgcga gtacctgcgg gaggcgttcc cggacgcgat acaagtctcc
     5701 gccaagaccg gcgagggagt cgaggagttg cgggatctac tcgaggaacg gctacgcgta
     5761 ctcgacgacc ggcaagaaga gcagccggag cggcgggaac accgtatttt ccgcccgaag
     5821 tggaagggtg tgagggtgca gcgagagaac ggcggttacg tcgtcgcagg cgagggagtc
     5881 gagcggctcg cgttgaagac ggactgggag aactctgagg gcgtcgagcg cttccagcgg
     5941 gagctcgaaa ggagcggggt cgtgagcgcc ctgcgacgcg cgggcgcgca acctggcgac
     6001 gaggtcagga tcggcgagac ggtgttcgac ttccggtgag gtgtcggctt tgaaagcggg
     6061 gatcttcggt gggaccttcg atcccatcca ccaggggcac atggtcatcg ccgagcaggt
     6121 gatgggtgag ctcggcctcg accacatcct gttcgtgccg gctggtatcc caccgcacaa
     6181 ggaagcctcc agcgtgcgag caagaactga agaccgctac gagatggtgg aggccgccgt
     6241 ggagggcaac gagcggttct ccgtggacag ggtcgaggtc gacgcaggac gccccatgca
     6301 cagcgtagag actgttcaga tcctcaaaga gcgatccccg gacgacgagt ggttcttcat
     6361 caccggggcg gacgaagtct ccaatttgct cgcgtggaag gacccggacc ggctgctcga
     6421 agaggtggtg atggtggcgg cgacacgacc cggctacgac ctttcgaggt tggatcacct
     6481 cgaggcgaag ctgaggaact tcgaccggat cttccccgtg gagtgcacgc gggtcgacat
     6541 ctcggcgacc ggtatccggc gccggatctt gcagggcaag agcgtacggt acctggtgcc
     6601 ggaagaggtc tacgagatca tcgtggaaag aggattgtac gaaggtgatg aaaaacggag
     6661 tggacaagga gggcgtttga aggaggagac gcggtgagcc gcgacgagtc agccatcagg
     6721 cgagagagcc tcgagacgga tggcgaagcg ctgagttacg agatggcgac cacggcggct
     6781 cgggcggcga gcgagatgtt cgccagggac gtgacgatcg tggatctgag ggacctggtc
     6841 tcgtatacgg actacttcgt aatagccagc gcggagaccg agcgccagac caggcgcgtg
     6901 gcccaggaag taatacagag gatgatcgag gctgggcacc gcccgcgcac caagcgcatc
     6961 gaagagggca cgacctggat cagcctcgac ttcctcgacg ttgtcgtcca cgtcctcacc
     7021 gacgaggccc gcgactacta ccgcttggag tccttctggc gcagcgcccc tcaggagcac
     7081 tgggaggagt agaccgagtc tctatgcgga acgagaaaca ccgcgttgac actccccatc
     7141 gcggtacata tactctgaca gttgcttttg gggccgtagc tcagccggga gagcgccgcg
     7201 ctggcagcgc ggaggtcagg ggttcgatcc ccctcggctc cactgcacga ctgtcctcag
     7261 gcgagtcgca tcgcgtagaa catactaccg ctgtacttgt ccgacagagc agtagctgca
     7321 cttcggggtc gaaagccatc ctgcgcttca ccaccgctat cagcctccac tcgacgtagg
     7381 cctggtacag gcccaatttg ccatctccct
//
LOCUS       DASTUV010000053         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_816173, whole genome shotgun sequence.
ACCESSION   DASTUV010000053 DASTUV010000000
VERSION     DASTUV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816173"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..964
                     /locus_tag="VFT03_01185"
     CDS             74..964
                     /locus_tag="VFT03_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU4846782.1"
                     /translation="MRFNFGFFMREALKNLRLNLLMSITAITTTFICIVVLGVAWLAS
                     AHVGAVTSSVRQDVSVEAYFPPDTTQEEIDGIISDVKSWPEVSGVSYVSEEEALAAFK
                     ETFADRPELSENLEPGVLPASIQIQLEDPSEAEAVAQRLEQEKGFTDDNLSYPQQTIE
                     RLNSVTSYMIWGLYGATMLFLVSSVLLISNAIRLSIFARRKEIEVMKLVGASDSFVRT
                     PFVFEGMVQGLIGAGLAAFAVIWINFLFIDWARDALPFVPISADAVNPIVILLALVVV
                     GVVIGVVGSFLSVTRFLRKV"
     gene            1170..2783
                     /locus_tag="VFT03_01190"
     CDS             1170..2783
                     /locus_tag="VFT03_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEU4846783.1"
                     /translation="MILPAVLVRRGKYSVADPLFVEERRPVLVARKLRRGAEAGDIVL
                     VNAREKNRSLRGEVTRVIGPSNDPRNAYEALFASIATSRRFPRKVEGEAEVVAGKDPG
                     ERRDLRHLPTVTIDGMDAKDFDDAISVERAGEGYRLWVHIADVTHYVEPGGALDEQAA
                     YRGNSVYLPGTVAPMLPERLSNDVCSLKPHVERAAVTVEMEVAPDGETRSATVFRSLI
                     TSDARLTYEGVDAFLRDEGEVVLPAMIRAAYELSLRLKTRAARRGKLELSGRETEFEI
                     DDEGIPVSARTRRSTPARDLIEELMVLANESVARMVRRKGKGIFRVHEKPDAESLELL
                     EERLAAIGVQEEPTPENLGVIAQRAKSDAVNYLILRSIPRALYSPQPSGHYGLALEDY
                     THFTSPIRRYADVIVHRALLGDEMPEDLSGIAEHISEREYRSMICERTADEYTLMWLM
                     QDRVGEVLEGTVVSTAAFGLFVELETGATGLVHVSKLPGWWELEPNGVVLSNKTIGSS
                     YKVGDRILVELLDVLPLMQRAELRVVKRL"
     gene            2780..3232
                     /gene="smpB"
                     /locus_tag="VFT03_01195"
     CDS             2780..3232
                     /gene="smpB"
                     /locus_tag="VFT03_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU4846784.1"
                     /translation="MTDFARNRKAFHDFDIDETYEAGIALTGPEVKSVRAGRANLKES
                     YARVRDGEVFLLGAHISPYGNATNIPQHPTRDRKLLLHRGEISRLAGKSQEDGKTLIP
                     LRLYDRNGNIKLELAVASRKRQYDKRREIARRTAEREMERAMKERLRR"
BASE COUNT          613 a         1044 c         1093 g          572 t
ORIGIN      
        1 gtgatgcgca agagggtcgt agccctcgaa gaggggcgcg tgatcaggga caaggtgagg
       61 ggggcatatg ccgatgaggt ttaatttcgg gttctttatg cgggaggcgc tcaagaacct
      121 ccgcttgaac ctcctgatga gcatcaccgc cataacgacg acgttcatct gcatcgtggt
      181 ccttggggtc gcatggctgg caagcgcgca cgtcggagcg gtgacctcct ccgtgcgcca
      241 ggacgtgagc gtcgaggcgt atttcccgcc ggacaccacg caggaggaga tagatggcat
      301 aatctccgac gtcaaaagct ggcccgaagt ctccggcgtc agctacgtct ccgaggagga
      361 ggcgctcgca gctttcaagg agaccttcgc ggacaggccg gagctctccg agaacctgga
      421 acccggcgtc ctgccagcgt cgatccagat ccagctggag gaccccagcg aggccgaggc
      481 cgtggctcag aggctcgagc aggaaaaggg gttcactgac gataacctga gctatccgca
      541 gcagactatc gagcggctca actcggtcac ctcgtacatg atctggggtc tctacggcgc
      601 cacgatgctg ttcctggtct ccagcgtgct cctgatctcc aacgccatcc ggctctccat
      661 ctttgcccgt cgcaaggaga tcgaggtgat gaagctcgtc ggggcctccg acagcttcgt
      721 gcgcaccccg ttcgtcttcg aggggatggt gcagggtctg atcggggctg gtctggccgc
      781 ttttgccgtg atctggataa actttctgtt catcgactgg gcgcgcgacg cactgccctt
      841 cgtcccgatc tccgccgatg cggtaaaccc catcgtcatc ctgctggccc tggtggtcgt
      901 gggcgtggta ataggcgtcg tcggcagctt cctctccgtg acacggttct tgaggaaggt
      961 ctagagaagg cttcacaggc agcggcagat aggtcttctg agaccgtgct gggtcgaggc
     1021 cacggtcctg gaggcgctct tataaaccac cctgcgcttc gggagggctt ccgacagcgc
     1081 acgcgaatcc tcgacggcga gccctgaagc ctttaatccg tcgcgtgata gactcaggac
     1141 gtgctctttt ctgagacagg agtcctttct tgattttgcc ggccgtgctg gtgcgccggg
     1201 gcaagtactc ggtcgccgac ccgctcttcg tggaggagag gcggcctgtg ctggtcgccc
     1261 gcaaattgcg ccgcggcgcc gaggccgggg atatcgtgct ggtgaacgcg cgcgagaaga
     1321 accgctcgct gcgcggcgag gtgacgcgcg tgatcgggcc ctccaacgac ccgcgcaacg
     1381 cctacgaggc cctcttcgcc tccatagcta cctcccgaag gttcccgcgc aaggttgaag
     1441 gcgaggccga ggtagtcgca ggcaaggacc cgggcgagcg cagggacctc cgccacctcc
     1501 ctaccgtcac catcgacggg atggacgcga aggacttcga cgacgccatc tccgtcgagc
     1561 gggcggggga gggatacagg ctctgggtac atatcgccga cgtgacccac tacgtcgagc
     1621 caggcggcgc tcttgacgaa caggccgcct accggggcaa ctcggtttac ctgcccggca
     1681 cggtcgcgcc gatgctcccc gagcgcctct ccaacgacgt gtgctccctg aagccgcacg
     1741 tcgagcgggc cgccgtgacg gtcgagatgg aggtcgcgcc cgacggcgag acgaggagtg
     1801 cgacggtctt caggagcctc atcacgagcg atgcgcggct cacctacgag ggagtagacg
     1861 ccttcttgcg cgatgagggt gaggtagtgt tgccggccat gatccgggcc gcctacgagc
     1921 tctcgctcag gctgaagacg cgggccgcca ggcgaggcaa gctggagctc agcggccgcg
     1981 agacggagtt cgagatagac gacgagggga tccccgtctc cgctcggacg cgccgctcga
     2041 cgccggctcg agatctcata gaggagctga tggtgctcgc caacgagtcg gtggccagga
     2101 tggtgcgccg caagggaaag ggtatcttca gggtgcacga gaagccggac gccgagtcgc
     2161 tggagctgct cgaggagagg ctcgcggcga tcggagtcca ggaggagccg acgcccgaga
     2221 acctgggggt gattgcccag agggccaagt ccgacgctgt gaactacctg atcctgcgct
     2281 ccatcccgcg cgcgctctac tcgccccagc cgagcggcca ctatggcctc gctctcgagg
     2341 actacacgca cttcacctcc ccgatccggc gctacgcaga cgtgatagtc caccgcgcgc
     2401 ttcttggaga cgagatgccc gaggacctct ccgggatcgc cgagcacatc tcggagagag
     2461 agtatcggtc catgatctgc gagcgcacag ccgacgagta cacgctgatg tggcttatgc
     2521 aggaccgcgt cggggaagtg ctggagggta ccgtggtgag taccgcggct ttcggcctct
     2581 tcgtcgagct ggagaccgga gcgacaggcc ttgtccacgt cagcaagctc cccggttggt
     2641 gggagctcga accgaacggt gtcgttctct caaacaaaac catcggctct tcctacaagg
     2701 tgggagacag gattctcgtc gagctgctcg acgtcctccc gctcatgcag cgggccgagc
     2761 tgagggtggt gaagcgcctg tgacggactt cgcgcgcaac aggaaagcct ttcacgactt
     2821 cgacatcgat gagacctacg aggcgggcat cgcgcttacc ggccccgagg tgaagtccgt
     2881 gcgtgcaggc cgggcgaacc tgaaggagag ctacgcgcgg gtgcgcgacg gggaggtctt
     2941 cctcctcggc gcccacatct cgccctacgg gaacgccacg aacatccccc agcaccccac
     3001 ccgcgaccgc aagctgttgt tgcaccgtgg cgagatctcc cgccttgcag gcaagagcca
     3061 ggaggacggc aagaccctca tccccctcag gctctacgac aggaacggca acatcaagct
     3121 cgagctcgcc gtcgccagcc gcaagcggca atacgacaag cgccgcgaga tcgcccgccg
     3181 taccgccgag cgcgagatgg agcgcgccat gaaagagcgc cttcgccgct agaaactgtt
     3241 ttgcaaagga cgatcggcgg actcatggtc ggttgggagg ctacgatcag agtgtccgtt
     3301 tcccgagatt ccggagaccg tc
//
LOCUS       DASTUV010000054         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1602987, whole genome shotgun sequence.
ACCESSION   DASTUV010000054 DASTUV010000000
VERSION     DASTUV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602987"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFT03_01200"
     CDS             <1..652
                     /locus_tag="VFT03_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846785.1"
                     /translation="SELEGRIIGLEESLEESERLRGEMESELANFRTRVESGELLRPN
                     GAPREGRETEDEQDGRLRELDAERLLAEERISDLEARLREAQEESRQNARDLAEALEA
                     LERLSDPERRLRDGIALFNASDHARAVASISKALGLPRVYAGLHGEAPGKPILTFAWG
                     DIAWRRYVSDPTEGVEEPRVYLTGTGDDPAEIEGAGQHPNARMDAQGRLMLGVQAR"
     gene            complement(703..1143)
                     /locus_tag="VFT03_01205"
     CDS             complement(703..1143)
                     /locus_tag="VFT03_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846786.1"
                     /translation="MLRSITAVALGVLVALAIGLLVVFGIFAPVFTAFFGMEMAASTA
                     VPTAMLVFAAAFSFYFGGMAASYTAPARRRLHGILVAPVAFVISPIINVATGQAMFPS
                     VDDPGTVLLAALFLGVSLAAAYIGARRGDTLFAYNRRMKRRAPR"
     gene            1481..2926
                     /locus_tag="VFT03_01210"
     CDS             1481..2926
                     /locus_tag="VFT03_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU4846787.1"
                     /translation="MQRTLTRRQFLKAPGAAGATLLGASALASGCIPQLNRSIGDTNV
                     VLVIIDSLRKDHVGAYGNRWIQTPNLDALAKESLRFTRAYPESLPTICARRAIHTGMR
                     TWPFRGWRRYKGIDIGLWGWQPIPEGQTHLAEIMKQNGYATLTVTDNLHEYKASMNFQ
                     RGFDVFDFIRGQTTDGYMPNWTYPPEEVRRALGMEEYFKQYFANVAYRQEEEDWFSPQ
                     VFTRASEVLEAASKGQRFFLVVDNYDPHAPYDAPEEYAALYDEPYDGREPFSPVYGPS
                     SYLTDRQLQRMRALYAGEVTMMDRWLGRFLDRMDELDLVENTLLVLLSDHGVAHGEHG
                     ITGKPPYALWPEVTDIVYMVRHPEGSRGGETSDHYASTHDVAPTILGFLGIEPEQPME
                     GQDLSVLFDGEEPGERSHFTAGYHNFVWARDSGYAMVSRQDRENARLYDLRRDPEMQQ
                     DISAGRPDVVRQMFDKYVLGDAGGPLPDYDG"
     gene            3210..3899
                     /locus_tag="VFT03_01215"
     CDS             3210..3899
                     /locus_tag="VFT03_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU4846788.1"
                     /translation="MDAEHVTRKSRRSKKTLREPVSGLTHAVGGVLAVVGLGVLLATA
                     ASTGRLDQLLAFGIFGLSLVALYAASALYHLLPLSPAGVARLRRLDHMTIFVLIAGTY
                     TPFCLLALDGGWRWGLLALIWSLALCGVMLKVRWMDAPRWLSVALYLGMGWIAVIAAS
                     ELLRAVPPGGIAWVLSGGLVYSAGALAYGLKRPNPVPGVFGFHEVWHIFVLAGSACHF
                     WAVLHYIAPLA"
     gene            complement(3920..4441)
                     /locus_tag="VFT03_01220"
     CDS             complement(3920..4441)
                     /locus_tag="VFT03_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU4846789.1"
                     /translation="MRLDGKKIVVLVAEGFEDLEYWVTVMRLREEGAAVISVGTSLDP
                     VYGKNALQAQPDATAEDVDASELDGIVIPGGWAPDKLRRYPDITGLVRAVYEADKTVG
                     IICHGGLVAISAGIVDGVRATGSLGIKDDLENAGATWVDKPAFREGNLVWGRVVPDIP
                     PFCRELVAALAEQ"
BASE COUNT          869 a         1483 c         1426 g          761 t
ORIGIN      
        1 ctccgagctc gagggtcgga tcatagggct cgaggagtcc ctcgaagaat ccgagcggct
       61 caggggggag atggagtccg agctcgcaaa cttccgtacc cgcgtcgaga gcggtgagct
      121 cctgcggccc aacggtgccc cccgcgaggg aagagagacc gaggacgagc aagacggtcg
      181 cttgagggag ctggacgccg agaggctcct ggccgaggag aggatcagcg acctggaggc
      241 tcgcttgcgc gaagcccagg aagagagccg gcagaacgcc cgagacctgg cggaggcact
      301 cgaggctctc gagcggctat cagatcccga gcgccggctc agggacggaa ttgcgctgtt
      361 caacgccagc gatcacgccc gggccgtcgc ttcgatatcc aaggccctcg gcctcccgcg
      421 agtctacgcc ggactgcacg gggaagcccc aggtaagccc atcctcacgt ttgcctgggg
      481 agatatagcc tggcgccgct acgtctccga cccgactgaa ggcgtcgagg agcccagagt
      541 ctacctgaca gggaccggcg acgaccccgc tgagatcgag ggtgccggcc aacacccaaa
      601 cgcccgcatg gacgctcagg gaaggttgat gctcggcgtc caggcacgct gacaagccgc
      661 cgaccgaagg gaggtggcgt gctaagaagg gaggcgtgct tatcagcgcg gcgcgcggcg
      721 cttcatgcgc cggttgtagg cgaagagcgt atccccgcgg cgggctccta tgtaggccgc
      781 ggccaaggag acgccgagga agagggcggc caggaggacg gttcccggat cgtccacgct
      841 gggaaacatt gcctgcccag tagcaacgtt gatgattggt gagataacga aagctaccgg
      901 ggcgactagt atgccgtgca agcggcgtcg ggcgggcgcc gtgtagctcg cggccatgcc
      961 cccgaaatag aacgagaagg ctgccgcgaa caccaacatg gcggtcggga cagccgtcga
     1021 ggcggccatc tccatcccaa aaaacgccgt gaaaaccggc gcgaagatgc cgaacaccac
     1081 gagcagcccg atcgcaagcg cgacgagcac ccctaacgcc acggcagtga tgctcctcaa
     1141 catgaagtta ttctatcaag cgcgcccgta gccctgcgtg aagccggcca ctagccaggg
     1201 tcttgtgaga cgacggacgg gtcctgttaa gctggcggga tgcttctgaa gggtgactcc
     1261 gattcacgcg cgtactccga gcgctaatcc cgagatccag aaagctcctc ggcacccata
     1321 acagctaccg gtcgaccccc accaacgtgc gtccgcctcg ggatatgtgc acaaacctcg
     1381 gccaccaaac agaggccctc gacttcatcc tgacagaggg cccgattgag ctagaatagg
     1441 ccgcggcgaa gcaggaccgc tcgggagact acggagcata gtgcagagaa cgctaacgcg
     1501 gaggcagttc ctcaaagcac ccggggctgc cggggcaacc ctgctcggtg cctcggcgct
     1561 cgcctccggg tgtatccctc agttgaacag gagcatcggc gacaccaacg tcgtcctggt
     1621 catcatagac agcctgagga aggatcacgt cggagcctac ggcaaccgct ggatccagac
     1681 cccaaacctc gacgctttgg ccaaggagag cctgcgcttc actagagcct accccgagtc
     1741 gttgcccacg atctgcgcgc ggcgcgcgat acacacgggg atgcgcacct ggcctttcag
     1801 gggctggcgg cgctacaagg gcatagacat cggcctctgg ggctggcagc cgataccaga
     1861 gggacagacg cacctggctg agataatgaa gcagaacggc tacgcgaccc tgacggtcac
     1921 cgataacctg cacgagtaca aagcctctat gaacttccag cggggcttcg acgtcttcga
     1981 cttcatccgg ggccagacca ccgacggcta catgcccaac tggacgtatc ctcccgagga
     2041 agtgagacgg gcgctcggaa tggaagagta cttcaagcaa tacttcgcca acgtggctta
     2101 ccggcaggag gaggaggact ggttctcgcc gcaggtgttc acccgcgcct cggaagttct
     2161 ggaggctgcg agcaaaggtc agcgtttctt cctggtggtg gacaactacg accctcacgc
     2221 gccctacgac gcgcccgagg agtacgctgc cctctacgac gaaccctacg atggcaggga
     2281 gcccttctca cccgtctacg gacccagcag ctacctgaca gaccgccagc tccagcgcat
     2341 gcgagccctc tacgctggcg aggtcacgat gatggaccgc tggctcggcc gtttcctgga
     2401 caggatggac gagctcgacc tcgtcgagaa caccctgctc gtcctcctct ccgatcacgg
     2461 cgtcgcccac ggcgagcacg gtatcaccgg caagccgccc tacgccctgt ggcccgaggt
     2521 cacggatatc gtctacatgg tccggcaccc cgaaggctcg agaggtggag agaccagcga
     2581 ccactacgcc tccacccacg acgtggctcc aaccatcctg ggcttcctcg gcatagagcc
     2641 cgagcagccc atggaaggcc aggacctctc cgtgctcttc gatggcgagg agcccgggga
     2701 acggtcgcac ttcacggccg gctatcacaa cttcgtgtgg gccagggaca gcgggtacgc
     2761 catggtctcc cgccaggatc gtgagaacgc aaggctttat gacctgcgga gggaccccga
     2821 gatgcagcag gatatctcgg ctggtcgccc agacgtggtg agacagatgt tcgataagta
     2881 cgtgctcggg gacgcgggcg ggcctttacc tgactacgac ggctgaagca gaagctgcca
     2941 agcgcaggcg agcttcgtca atcacagaac tccctgcaaa gtatacggag gagtggccgg
     3001 tgctcacatt actacgaatg cttactcgct gccctgattg ctttaaacga ggatgacaga
     3061 cgacgatgaa tcaagcccgg caggcgtccc cgaaccgtcg taaggtcgac cgacggctta
     3121 ccggttatga gctcgacaat ctgccgggct gttccacttc ccggttctcg ctggttaggg
     3181 tacccttacc ctcgagggta ggcgatcgga tggacgcaga gcacgtgacg cgtaaaagtc
     3241 gcaggtcgaa aaagacgttg cgggaacctg tcagcgggct cacccacgcg gtgggaggag
     3301 tgctcgcggt cgtggggctg ggcgtgctgc tggcgacggc ggcgagcaca gggaggctgg
     3361 atcagctcct cgccttcggc atcttcggtc tgagcctggt cgccctctac gccgcgagcg
     3421 ccctctacca tcttctcccg ctctctcccg ctggagtagc gaggctgcgg agactggacc
     3481 acatgaccat cttcgttctc atagcgggca cgtacacccc attctgcctg ctcgcgctag
     3541 atggcggatg gaggtggggt ttgctggccc ttatctggag cctcgcgctg tgcggcgtca
     3601 tgctcaaggt ccgctggatg gacgccccgc gctggctctc ggtagcgctc tacctcggca
     3661 tggggtggat cgccgtgatc gcggcctcag agctccttcg agcggtgcca ccaggcggca
     3721 tcgcctgggt cctgtccggc ggcctggtct acagcgcggg cgcgctcgcc tacgggctca
     3781 agcgccccaa cccggttcct ggagtcttcg ggttccacga ggtatggcac atcttcgtcc
     3841 tggcgggaag cgcctgccat ttctgggcag ttctacacta catcgcgccg ctggcctagg
     3901 gctccagccg cggattatcc tactgctccg cgagcgccgc tacgagctcg cggcagaagg
     3961 gcgggatgtc ggggacgacg cggccccaga ccaggttccc ttcgcggaag gccggcttgt
     4021 ccacccaggt cgctccggcg ttctccaggt cgtcctttat gccgagggag cccgtcgccc
     4081 tgacgccgtc cactatgccg gctgagatcg cgacgagccc gccgtggcag atgatgccga
     4141 cggtcttgtc ggcctcgtac accgccctga cgaggccggt gatgtccggg tagcgccgta
     4201 gcttgtccgg tgcccagccg ccgggtatga ctataccgtc gagctcagag gcatccacgt
     4261 cctccgccgt cgcgtccggt tgggcctgca aggcattctt gccgtatacc gggtccaggc
     4321 tcgtcccgac cgagatgacc gccgcaccct cctcccgcag ccgcatcacc gtgacccagt
     4381 actccaggtc ttcgaacccc tcggccacca gcaccacaat cttcttcccg tcaagcctca
     4441 tccccatctc ctttctatta gcatttcagc aattcagcta gtcggcttca ggagttcgtt
     4501 tacggcctcc tttgctgcgt cgcctccgag ctccagggc
//
LOCUS       DASTUV010000055         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_922437, whole genome shotgun sequence.
ACCESSION   DASTUV010000055 DASTUV010000000
VERSION     DASTUV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922437"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFT03_01225"
     CDS             <1..326
                     /locus_tag="VFT03_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846790.1"
                     /translation="AGGNFKRSTAEIISELLSDERVRRGLDYVRSDEFRAERERIRQG
                     AADRLKDVRERYRPKRRTPEAAALERELSLRLAEVEAEAAELRIRLSELLEQEEEIRT
                     ALEDL"
     gene            580..>4309
                     /gene="gltB"
                     /locus_tag="VFT03_01230"
     CDS             580..>4309
                     /gene="gltB"
                     /locus_tag="VFT03_01230"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEU4846791.1"
                     /translation="MTLGTGLYDRTYDHDACGLGFVARVDGRRTRETVEEGLEVLRNL
                     EHRGTSGSDPETGDGAGILTQIPDALFRREAGDLGIELPPAGLYGVGTLFEYGAEEGR
                     DCEERLERIVAEEGQQFLGFRDVPIEAEYAGRLARKVMPRIRQFFVERRNGDPEGFER
                     KLYVIRRRLHDAVQDTHSGYVVNLSSRTIVYKGLLKGQQLPRFYPDLADPAFASAIAL
                     VHERFSTNTLGSWELAHPYRYVAHNGEINTLRGNINWMRARESRLESPLFGEDMKKLS
                     PIIQPGQSDSASFDNALELLYLAGRSLPHAVAMMIPEAWENDDLMEEERRAFYQYHSA
                     LMEPWDGPAAIAFTDGRVVGATLDRNGLRPARYSVTKDGRVIMASEDGALRVPAEDVV
                     ERWRLRPGRMLVVDTERHELLHDEDVKRPLFRRQPYGKWLKEEEIHLNELPDATPDGR
                     PEPSNLFERQLAFGYTIEDLRLLLSPMAREGKEPDGSMGTDTPLAVLSERPELLFSYF
                     KQHFAQVTNPPIDPLREELVMSLKMSLGPEQNLFDETPEHCRRVLIDQPVLTADELEK
                     IRRLGSERAAASTLSTLFPVSVGERSMEEALHRLCEKAESAVKGGSAVLVLSDRGVSP
                     EQAAIPSLLATAAVHHYLVRAGIRTGTTLVVETAEAREVHHFALLVGYGATAVNPYLA
                     FETLEVMAGSNLLEGVGVEEARKNYVKAIGKGLLKVISKMGISTLFSYCGAQIFEAVG
                     LNQEVIDRYFTGTASRVGGVGLEEIEREVLERHRRAFSPVEDGPEELEVGGEYQLRMQ
                     GQYHQWNQTTIPPLQRAFKTGSFETFKEFTRHFDEQSARFSTLRGLFEFEENPIPLEE
                     VEPAREIVKRFTTGAMSLGSLSREAHETLAIAMNRIGGKSNTGEGGEDPVRFTPDENG
                     DSRRSAIKQVASGRFGVTTEYLVNADQLQIKMAQGSKPGEGGQLPGHKISEYIARIRY
                     STPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARVSVKLVAEVGVGTVAAGVAKA
                     KADHITISGHDGGTGASPLSSIKHAGLPWELGIAETQQVLVENGLRGRVILQTDGQLK
                     TGRDVTVAALLGAEDFAFSTAPLVATGCIMMRVCHLNTCPVGIATQDPELRKRFTGTP
                     EHVINYFFFVAEEVREYMARMGFRSFEEMIGRADRLRMREAVEHWKASGVDLSAILHR
                     VEAPEGEEIHHSQEQDHNLEKSMDNELIEKCLPALENNE"
BASE COUNT          825 a         1366 c         1461 g          657 t
ORIGIN      
        1 tggccggcgg gaacttcaag cgcagtacgg cggagatcat ctctgagcta ctctccgacg
       61 agagggttcg ccgcggactg gactacgtcc ggagcgacga gttcagggcg gagcgggagc
      121 gcatccggca gggcgccgcc gaccgcctga aagacgtccg cgagcgctac cgcccgaaga
      181 ggcgcacccc cgaagcagcc gccctcgaga gggaactctc cctgcggctc gccgaggtcg
      241 aagccgaagc cgccgagttg aggatccggc tctccgagct cctcgagcaa gaggaagaga
      301 tccggaccgc actcgaagat ctttaagctt caagttacag gtttcccgct cggccgacct
      361 tggttccgct acagatacga gagagttagc ggcttcccgc ccacgagact acgttatcgc
      421 tgagagctag tccagcacca cctgatgcct gaagcctgaa acctttccag aagccttgta
      481 cgacactagt atgttactgc ttgctccccc ttgtgattta cgggcgattc gtgtaaaaat
      541 gggccctccg caccccgtag cgctacagga ggcagcctgt tgacccttgg aaccggcctt
      601 tacgaccgca cgtacgatca cgatgcctgc gggctcgggt tcgtagcgcg tgtagacggt
      661 cggcgcacgc gcgagacggt ggaggagggg ctcgaggtgc tgcgcaacct cgagcaccgg
      721 ggcacgagcg gcagcgaccc ggagacgggg gatggggctg gtatcctgac ccagatcccc
      781 gacgccctct tcaggcgcga ggccggggat ctcggcatag agcttccgcc cgcggggctc
      841 tacggggtcg gcactctgtt cgagtacggg gcggaggaag gccgcgactg cgaggagcgg
      901 ttggagcgga tcgtcgccga agaggggcag cagttcctgg gcttcaggga cgttcccata
      961 gaggcggagt acgccgggag gcttgcgcgc aaggtcatgc cgcgcatccg gcagttcttc
     1021 gtcgagcgca ggaatggcga cccggagggc ttcgagcgga agctgtacgt gatccggcgg
     1081 aggctccacg acgcggtgca ggacacgcac agcggttacg tcgtgaacct ctcttcgcga
     1141 accatcgtct acaaggggtt gctcaagggg cagcagttgc cgcggttcta tccggatctc
     1201 gcggatcctg cgttcgcgag cgcgatcgcg ctggttcacg agcgcttctc gacgaatact
     1261 ctgggtagct gggagctcgc gcacccctac cgctacgttg cgcacaacgg tgagatcaac
     1321 accctgaggg gaaacatcaa ctggatgcgc gcccgcgaga gccgcctgga gtcacccctc
     1381 ttcggggaag acatgaagaa gctctccccg atcatccagc cgggccagag cgactcggcc
     1441 tccttcgaca acgccctgga gctcctctac ctcgccggcc gcagcctccc acatgccgtg
     1501 gcgatgatga tccccgaggc gtgggagaac gacgacctga tggaggagga gcgcagggct
     1561 ttctaccagt accacagcgc cctcatggag ccgtgggacg gccccgcggc catcgccttc
     1621 acagacgggc gcgtcgtcgg cgcgaccctg gacagaaacg gactgcgccc ggcgcgctac
     1681 tccgtgacga aggacgggcg cgtcatcatg gcctcggagg acggggcatt gcgcgtcccg
     1741 gccgaggacg tcgtcgagcg gtggaggctg cggccgggcc ggatgctggt cgtggacacc
     1801 gagcggcacg agcttctgca cgacgaggac gtcaagaggc cgttgttccg gcggcaaccg
     1861 tacgggaagt ggctgaagga agaggagatc cacctcaacg agctgcccga cgcgacgccg
     1921 gacgggcgtc ccgagccatc gaacctcttc gagcggcagc tcgctttcgg gtacacgata
     1981 gaggacctga ggctactcct ctccccgatg gctagagaag gcaaggagcc ggacggctcg
     2041 atgggcaccg acacgccgct ggccgtcctc tccgagaggc cggagctcct gttctcgtac
     2101 ttcaagcagc acttcgcgca ggtcacgaat cctcctatcg accccttgcg cgaggaactg
     2161 gtcatgtcgc tcaagatgtc gctgggtccg gagcagaacc tcttcgacga gacgccggag
     2221 cactgccggc gcgtcctcat agaccagccc gtcctgaccg ccgacgagct cgagaagatc
     2281 cggcgcctcg gctccgagcg ggccgccgcc agcacgctct cgaccctctt ccctgtctcg
     2341 gtgggggaga ggagcatgga ggaggccctg cacaggctgt gcgagaaggc cgagagcgcg
     2401 gtaaagggtg ggtccgcggt cctggtgctg agcgacaggg gcgtgagccc ggagcaggcc
     2461 gcaataccga gcctgctcgc cacggccgcc gtccaccact acctggtgcg ggcgggcata
     2521 cggaccggca cgacgctggt cgtcgagacg gccgaggccc gcgaggtaca ccacttcgcg
     2581 ctgctcgtgg gctacggcgc gacggccgtc aacccgtacc tcgctttcga gacgctggag
     2641 gtgatggctg gctcaaacct gctcgaaggc gtgggcgtcg aggaggcccg caagaactac
     2701 gtcaaagcta taggcaaggg cctcctcaag gtcatctcca agatgggcat ctcgacgctg
     2761 ttctcgtact gtggggccca gatcttcgag gccgtcggcc tgaaccagga ggtcatagac
     2821 aggtacttca ccggcaccgc ctcgcgcgtc gggggcgtcg gactcgagga gatcgagcgc
     2881 gaggtcctcg agcggcacag gcgcgccttc tcgcccgtcg aggacgggcc cgaggagcta
     2941 gaggtcgggg gcgagtacca gctcaggatg cagggccagt accaccagtg gaaccagacg
     3001 acgatcccac ccctccagcg cgccttcaag acagggagct tcgagacctt caaggagttc
     3061 acccgccact tcgacgagca gagcgcgcgc ttctccacac tgcgcggcct cttcgagttc
     3121 gaagagaacc ctatacccct cgaagaggta gagcctgcgc gcgagatcgt caagcgcttc
     3181 acgacgggcg cgatgtcgct agggtcgctc tcgagggagg cgcacgagac tctggccatc
     3241 gcgatgaacc gcataggcgg caagtccaac acgggcgagg gtggcgagga ccctgtgagg
     3301 ttcacgcctg acgagaacgg cgacagccgc cgcagcgcca taaagcaggt cgcgagcggg
     3361 cgcttcgggg tgacgacgga gtacctggtc aacgcggacc aattgcagat caagatggcc
     3421 caggggtcca agccgggcga gggcggccag ttgccggggc acaagatcag cgagtacata
     3481 gcgcggatac ggtactcgac gccaggcgtg ggcctcatct cgccacctcc tcaccacgac
     3541 atctactcga tcgaagacct ggcgcagctc atccacgacc tcaagaacgc caacccggcg
     3601 gccagggtga gcgtgaagct cgtcgccgag gtgggggtgg ggacggtcgc ggctggtgtg
     3661 gcgaaggcca aggccgacca cataaccatc tctggccacg acggcggtac cggcgcctcg
     3721 ccgctctcct ccataaagca cgcggggctg ccgtgggagc taggcatcgc cgagacccag
     3781 caggtcctcg tcgagaacgg gttgcgcggg cgtgtgatcc tgcagaccga cggccagctc
     3841 aagacggggc gcgacgtaac cgtggcggca ttgctggggg ctgaggactt cgccttctcg
     3901 acggcgccgc tggtagctac gggctgcatc atgatgcggg tgtgtcattt gaacacctgt
     3961 cccgttggca tcgcgaccca ggacccggag ctcaggaaga ggttcaccgg cacgccggag
     4021 cacgtcatca actacttctt cttcgtcgcc gaagaggttc gggagtacat ggcgaggatg
     4081 ggcttcagga gcttcgagga gatgatcggg cgcgcggaca ggctcaggat gcgcgaggcc
     4141 gtcgagcact ggaaggcgag cggcgttgac ctctcggcca tcctgcacag ggtcgaggcg
     4201 cccgagggag aggaaatcca ccactcgcag gagcaggacc acaacctcga gaagtccatg
     4261 gacaacgagc tgatcgagaa gtgcctgccc gcgctagaaa acaacgaac
//
LOCUS       DASTUV010000056         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1497200, whole genome shotgun sequence.
ACCESSION   DASTUV010000056 DASTUV010000000
VERSION     DASTUV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497200"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..518
                     /locus_tag="VFT03_01235"
     CDS             69..518
                     /locus_tag="VFT03_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846792.1"
                     /translation="MGRESSTEARTGPGPALARAVFPERSWFEALGEAVAADAEMAVI
                     GRWCNLDLALVADEEMVLLRLREGRISEVVPNPGISYSWSVTLRGTRKDWLTFLQPEP
                     PPFYNDLLAMNGRIPSFSIEGDRAAFVRHLRTLSRLFHLAQLMGSHA"
     gene            511..1362
                     /locus_tag="VFT03_01240"
     CDS             511..1362
                     /locus_tag="VFT03_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4846793.1"
                     /translation="MPEFDPITGRYAYLDVAGSRNRVYFEEAGEGRPLLCLHTAGADS
                     RQWRHLLCDTEVTGRWRVLAFDMPYHGRSTPPAGWWEKEYLLTTETYARMIMAFVRAL
                     ELEQPVVLGCSMGGAVVLELASRHATEVSAVIGLEAAGKIEGRFSDWTVMPDVDGSVG
                     AASWTYGLMAPQSPEAARREVWWTYAQGGPGVYRGDTYFYSVDWDLRGREEEIDSDTC
                     PVYLLTGEYDHACTPQETAATAAAIPGAKFTRMRGIGHFPMAENYPRFREHLLPILKE
                     LEDAQEP"
     gene            1379..1798
                     /locus_tag="VFT03_01245"
     CDS             1379..1798
                     /locus_tag="VFT03_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU4846794.1"
                     /translation="MATQEEKQAYIDEMVRKRGYVLDYHKVMTRYDFDVLQAANGLVE
                     AAYLKERRLDRKTKELIFIVSLTVMRASKGHIQSHIRVALDLGLSKEEILEAIEIALP
                     EAGVVAFQEGFEAWREVTGAEGIEPSTQVHQGGSGSR"
     gene            complement(1849..>2052)
                     /locus_tag="VFT03_01250"
     CDS             complement(1849..>2052)
                     /locus_tag="VFT03_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1445 domain-containing protein"
                     /protein_id="HEU4846795.1"
                     /translation="PEALGIRDLSDPEFGEPVEFEEDQLPVFWACGVTPQAIAMKVKP
                     PLMITHSPGHMFITDRRHSEYEL"
BASE COUNT          371 a          633 c          709 g          339 t
ORIGIN      
        1 cggtgcggag ggccatacgg cgatctacgc cttcctcgag gaactcgctg taccaggaag
       61 gagtcgtaat gggtagagag tcgtccaccg aagctaggac ggggccaggt ccggcgctcg
      121 cccgggctgt gtttccggag cggagctggt tcgaggctct gggcgaggcg gtcgcggcgg
      181 atgccgagat ggcagtgata gggcggtggt gcaacctcga tcttgccctg gtggccgacg
      241 aggagatggt cctgctgcgc ctgcgggaag gccgtatctc cgaggtcgtc ccgaaccccg
      301 gcataagcta ctcctggagc gtgacgctac gcggcacgag aaaggactgg ctgaccttct
      361 tgcagccgga gcctcctccc ttctacaacg acctgctcgc catgaacggc cgcatcccgt
      421 cgttctccat agagggcgac cgtgcagcgt tcgtgcgaca tctgcgcacc ttgagcaggt
      481 tgttccacct cgcgcagctg atgggtagcc atgcctgaat ttgatccgat cacgggccgg
      541 tacgcctacc tggacgtcgc gggctcccgc aaccgcgtct acttcgagga agctggggag
      601 ggccggccgc tcctgtgcct ccacaccgcg ggggccgact cgcgccagtg gcggcacctg
      661 ctctgcgaca cggaggtgac cggtaggtgg cgagtcctcg ccttcgacat gccttaccac
      721 gggcggtcga cgccgcccgc gggttggtgg gagaaggaat acctgctcac gaccgagacc
      781 tacgcgcgca tgataatggc ctttgtgcgt gccctggagc tggagcagcc cgttgtgctc
      841 gggtgctcga tgggaggtgc cgtcgtgctc gagctggcga gcaggcacgc cacggaggtg
      901 agcgccgtga tcggcctcga ggccgccggc aagatagagg gacgcttctc ggactggacg
      961 gtgatgcccg acgtggacgg ctcggtgggc gccgcctctt ggacctacgg cctcatggcg
     1021 ccccagagcc cggaggctgc ccgccgggag gtgtggtgga cctacgccca gggcggcccc
     1081 ggtgtctacc gtggcgacac ctacttctac agcgtcgact gggacctccg cggaagggag
     1141 gaggagatag actccgatac ctgcccggtc tacctgttga ccggggagta tgaccacgcc
     1201 tgtacgccgc aggagaccgc cgcgaccgca gcggctatac ccggcgcgaa gttcacgagg
     1261 atgcgtggca tcggccattt ccccatggcg gagaactacc cacggttcag ggagcacctg
     1321 ctgccgatac tcaaagagtt ggaagacgcg caagagccat aacgaaaggg aggaaagcgt
     1381 ggcgacacag gaagagaaac aagcgtacat cgacgagatg gtccgcaagc gaggctacgt
     1441 cctcgactac cacaaggtga tgacgcgcta cgacttcgac gtgctgcagg ccgccaacgg
     1501 cctcgtcgag gcggcctacc tcaaagagcg gcgactcgac cgcaagacga aggagctcat
     1561 cttcatcgtc agcctcacgg tgatgcgggc gagcaagggt cacatccaga gccacatacg
     1621 ggtggctctg gatctcggcc tctccaaaga ggagatcctg gaggccatcg agatcgcgct
     1681 gcccgaggct ggggtcgtgg ccttccagga gggcttcgaa gcctggcggg aggttaccgg
     1741 ggcggagggg atcgaaccga gcacgcaggt ccaccagggc ggctccgggt cgaggtagcg
     1801 tccccggagc accccgtcca ctgaaggggc ttctacttca ggtaccgact agagctcgta
     1861 ctccgagtgt cggcggtccg tgatgaacat gtggcccggg ctgtgggtga tcatcagggg
     1921 aggcttgacc ttcatggcga tcgcctgggg ggtcacgccg caggcccaga acacgggcaa
     1981 ctggtcttct tcgaactcca caggctcacc gaactccggg tccgacaggt ctctgatccc
     2041 cagggcttcg gg
//
LOCUS       DASTUV010000057         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_279522, whole genome shotgun sequence.
ACCESSION   DASTUV010000057 DASTUV010000000
VERSION     DASTUV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279522"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFT03_01255"
     CDS             <1..895
                     /locus_tag="VFT03_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU4846796.1"
                     /translation="LLYAALGSDTGGSIRIPAALCGVVGMKPTFGRVSKRGVFPLSWT
                     LDHVGPLTRTVEDNALLLNALAGHDPADPYSSNRPAEDFARDLHRGVRGGVIGVPEDF
                     YFENLDGEVEKKVREAIAVFLASGAEMREVSIPCLQETLEAQRLTLAADAYAVHEERL
                     QREPERFGEEVRERLAHGERLEAHRYASAQQTKLRAKYSFERALEGVDILLTPTLPVA
                     ATPIDAREVDIGGHEESVRPAVTRLTGPTNLTGFPSLSVPCGSTAAGLPVGAQLIGRP
                     FDEATVYRFGHAYETAVAGEG"
     gene            971..1960
                     /locus_tag="VFT03_01260"
     CDS             971..1960
                     /locus_tag="VFT03_01260"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU4846797.1"
                     /translation="MKIAVLTGGGVAPGMNAAVRAVAQKAFSFGWEVLGVEDSYDGLL
                     KGRLSPIDRNRLGGLVHRGGTFLGTGRSKEVREPEGQERAVERLSEAGAEGIVIIGGG
                     GSLTSAKALGEAGVRAVGIPATIDNDVPGTELAIGVDTAVDTAVRAIDQVRGTAISHN
                     RAHIVEVMGRDSGYLALMSAIAGGAEVALIPEFQAEREDVLRFIEEAYERGKSHFIIV
                     AAEGAEPSAGEMQEYINGAEGTYEADLTAVGHIQSGGDPTSTDRILASRLGSAAVEAL
                     ADGEPGIMVGVRGDETVRFPLEEVVGEKRPLDPDMYELSKVLAEISDNANDSR"
     gene            2031..3572
                     /locus_tag="VFT03_01265"
     CDS             2031..3572
                     /locus_tag="VFT03_01265"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEU4846798.1"
                     /translation="MADGEGANNLDGRVTRQRFLEAAGAGAAWIALTSALGCEPSKLA
                     SGETPSSGRQGSANLPPDALSFRSRPDLRPPGVEVATPAHGTAPGYIFLAPKEGSARP
                     GPMIVDNAGQLVWFRPVHGEGVRAMDCKVQRYRGEPVLTYYEGTGMAYGRGEYVIFDS
                     SYREVRRVRAGNGYQGDHHEFLITPEGTALITIYSPVSWDLSPVGGPKDGAVLDGIAQ
                     EVDIESGEVLLEWHSLDHVGIEESYSVPEKNQSSPHDYFHINSIDVEPDGNLLVSSRR
                     TFTLYKIDRRSGEIIWRLGGKKSDFEMGEGTWMRYQHDARRHPDGTITVFDNGGVKKD
                     EQSYGLVLDADTSQMRASLVRHYAHPDKVLAATQGNVQVLENTNVFVGWGSEPVFSEF
                     SEDGKLLFDANLPPEVESYRAFRFVWSGEPADEEPAVAAELGEAEGELRVYASWNGAT
                     EVASWEVLSGPAPERLKPTVSASREGFETAITVLRGAGRYVAVRAKDNSGRVLGASKA
                     VEPGG"
     gene            complement(3584..3832)
                     /locus_tag="VFT03_01270"
     CDS             complement(3584..3832)
                     /locus_tag="VFT03_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846799.1"
                     /translation="MAIELSLFNNSVTTLVTHLTSYYGDDAELETYVLCVRVKSPDIP
                     GGSGLNWIVGPNGHERAPEVLRGVLEALENGESNGHGA"
BASE COUNT          739 a         1328 c         1383 g          691 t
ORIGIN      
        1 cctcctctac gctgctctgg gctccgacac ggggggctct atacgtatcc ccgccgctct
       61 gtgcggcgtc gtcggcatga agccgacctt cgggcgcgtg agcaagcgcg gcgtcttccc
      121 tctctcatgg accctggacc acgtcggccc cctcacccgc accgtcgagg acaacgcgct
      181 cctcctgaac gccctcgccg gccacgaccc cgcagatccc tactcctcga accgtcccgc
      241 cgaggatttc gcccgcgatc tgcaccgcgg tgtccgggga ggtgtgatcg gagtcccaga
      301 agacttctac ttcgagaacc tcgatggaga ggtggaaaag aaggtgaggg aggccatagc
      361 ggtcttcctg gcttcgggcg cggagatgcg ggaggtgagc atcccgtgtc tccaggagac
      421 gctcgaggcg cagcgtctca cgctggccgc cgacgcgtac gcggtgcacg aggagcgact
      481 gcagagggaa cccgagaggt tcggcgaaga ggttcgcgag cgcctcgcgc acggcgagag
      541 gctcgaggcg caccgctacg ccagcgccca gcagacgaag ctgcgcgcga agtactcctt
      601 cgagcgggcg ctggaagggg tggacatcct cctcaccccg acgttgccgg tagccgcgac
      661 gcccatagac gcgagggagg tggacatcgg gggccacgag gagagcgtcc gccctgcggt
      721 tacccggctt accggaccga cgaacctcac cggcttcccg agcctctccg tgccctgcgg
      781 ctcgaccgcc gccggcctgc cggttggcgc gcaactcatc gggcggcctt tcgacgaggc
      841 caccgtctac cgtttcgggc acgcctacga gactgctgtc gccggagagg gctagccccc
      901 ttctgtacaa gcgccgacgc gccgcgcagg gcatcatacg tcccgtgttc cgagtcggag
      961 ggagaaccgc atgaagatag ccgtcctgac cggcgggggc gtggccccag gcatgaacgc
     1021 cgccgtgcgc gccgttgcgc agaaggcgtt ctctttcggt tgggaggtcc tcggggtgga
     1081 ggacagttac gacgggctgc taaaagggcg tctcagccct atagaccgga accggctcgg
     1141 ggggctcgtg caccggggcg ggaccttcct cggcaccgga cgctccaagg aggtcaggga
     1201 gcccgagggg caggagcgcg cggtagagcg gctgagcgag gcgggcgcgg agggcattgt
     1261 gataatcggc ggagggggga gcctcacaag cgccaaggcg ctcggggagg ccggggtccg
     1321 ggctgtcggc attccggcga ccatagacaa cgacgtgcct ggcacggagc tcgccatcgg
     1381 cgttgacacc gccgtggaca cagctgtcag ggccatagac caggtcaggg gcacagccat
     1441 ctcccacaac agggcgcata tagtcgaggt catgggccgt gactccggct acctggcgct
     1501 gatgagcgcg atcgcgggcg gcgcagaggt cgccctcata ccggagttcc aagctgaacg
     1561 cgaggacgtc ttgcgcttca tcgaggaggc ttacgagcgg ggcaagtctc acttcataat
     1621 cgtggcggcc gagggagccg agccctcggc cggggagatg caggagtata taaacggggc
     1681 cgaagggacc tacgaggccg acctcacggc ggtcggccac atccagtcgg gtggcgaccc
     1741 cacatccacg gacaggatcc tggcctctcg tctcggctcc gcggcggtcg aggcgctcgc
     1801 cgacggcgag cccggcatca tggtcggggt gcggggcgac gagacggtgc gctttcccct
     1861 cgaagaggta gtcggcgaga agcgtcccct cgacccggac atgtacgagc tctccaaagt
     1921 gctcgccgag atatccgata atgcgaacga ttcgcgctga ggctccgtcc gggcgcgcgg
     1981 cccggaatag ggtacagtaa tggactttgg atacggtgtg cgaggtgtat gtggctgacg
     2041 gtgaaggagc gaacaacctg gacggtaggg tcacgcggca gcggttcctc gaagctgcgg
     2101 gggccggcgc cgcgtggatc gcgcttacga gcgctctggg ttgcgaaccg tccaagctcg
     2161 cctcggggga aacgccttcg tccggccgac agggctccgc taacctgccg ccggacgcgc
     2221 tatcgttccg gtcgcggcct gatctccggc ctcccggcgt cgaggtggca actcctgctc
     2281 acggcaccgc tcccggctac atcttcctcg ccccgaaaga gggctcggct cggcccggac
     2341 cgatgatcgt cgacaacgca gggcaactcg tgtggtttcg gcccgtacac ggcgaaggcg
     2401 tgagggccat ggactgcaag gtgcagcgct accgcggcga gcctgtcctt acctactacg
     2461 agggtaccgg tatggcctac ggtcgcggag agtacgtgat cttcgactcc tcctacaggg
     2521 aggtgaggcg ggttcgggcc ggcaacggct accagggcga ccaccacgag ttcctcatca
     2581 cccctgaggg cacggcgctg atcacgatct acagtcccgt gagctgggac ctctcgcctg
     2641 taggtggtcc gaaggacggc gctgtgctgg acgggatagc ccaggaggtg gacatcgaga
     2701 gcggagaggt gctcttggag tggcacagcc tcgaccacgt cggcatagag gagtcctact
     2761 ccgtgcctga gaagaaccag agtagccctc acgactactt ccacatcaac tccatagacg
     2821 tcgagcctga cggcaacctt ctcgtctcct cgcgcaggac cttcaccctc tacaagatag
     2881 accgccgcag cggcgagatc atctggcgcc ttggcggcaa gaagagcgac ttcgagatgg
     2941 gcgaggggac ctggatgcgc taccagcacg acgccaggcg ccaccccgat ggcaccatca
     3001 cggtcttcga caacggtggg gtaaagaagg acgagcagtc ttacggcctc gtgctagatg
     3061 ccgacaccag ccagatgaga gcttcactgg ttcgccacta cgcccaccct gacaaagtcc
     3121 tggcagccac ccagggcaac gtccaggtgc tagagaacac caacgtgttc gtagggtggg
     3181 gttctgagcc tgtcttctcg gagttcagcg aggatggcaa gttgctcttt gacgccaacc
     3241 tcccgccaga ggtggagtcc tacagggcct tccgctttgt gtggagcgga gagccggccg
     3301 acgaggagcc ggcagtggcc gccgaactcg gagaagctga aggcgagctg agggtatatg
     3361 cgagctggaa cggtgcgaca gaggttgcca gctgggaggt gctaagtggt cctgcacccg
     3421 agaggttgaa gcccacagtc tccgcctcca gggagggctt tgagacagcc atcacggtgc
     3481 tgcgtggtgc cggtcggtac gtcgccgtcc gggccaaaga caactcgggc cgcgtgctcg
     3541 gcgcctcaaa ggcggtcgag ccgggaggct aggcggcgtg ggatcacgcg ccgtgcccgt
     3601 tgctttcgcc gttctccaga gcctccagaa ccccgcgcag cacttctggc gcccgctcgt
     3661 ggccgttcgg gcctacgatc cagttcaggc cgctcccgcc cgggatatcc ggcgacttta
     3721 ccctcacgca gaggacgtac gtctcgagct ccgcatcatc cccgtagtag ctggtgaggt
     3781 gcgttaccag agttgtaacc gagttgttga acaacgacag ctctatcgcc acgtgaacct
     3841 ccgtattacg ctctctccgg attaccggac accttcaccg taccatctcg agtgccatct
     3901 ctggaacagg gtgggcggag acggggaagg ctaggaaggt tatagccctt gttcggactg
     3961 ctgttgcctt atctccagtt cctttccaca tgctccagtg ccggacactc tcctctacct
     4021 gcgtttgacc ttccggacat ctactgcgat acgccgaggc ggcgctgttc ccccttccgc
     4081 ggcgtcggca ctgaactact gcagctatcc aatccctacc tccggatgct accgaggctt
     4141 a
//
LOCUS       DASTUV010000058         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_28586, whole genome shotgun sequence.
ACCESSION   DASTUV010000058 DASTUV010000000
VERSION     DASTUV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28586"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1488)
                     /locus_tag="VFT03_01275"
     CDS             complement(154..1488)
                     /locus_tag="VFT03_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU4846800.1"
                     /translation="MPLDYTYRIQIPHRTGQFARVAGAIADGGGLIGDVTTINVGRES
                     SLREITLEINDRDQAERIVGMLNALEGVEVVWARDRALLKHEGGKLVIESAHPVRTVQ
                     DMRDVYTPGVARACEAIVEDPSLASELTMIGRSVAICTNGTRVLGLGDIGPVASMPVM
                     EGKAVFYQQLAGVSAMPILVDARDVDTFVETVVRIAPTFGGIHLEDISAPECFEIEAR
                     LIERLPQPVMHDDVHGTAVVTLAAALVACRQAGLDLREEVVGQIGLGAAGFGIAALMV
                     DAGAKRVLASDPNPLSHERASAKGIEITDMQTVMGEADVVVATTGVPGLIKPEMVRAG
                     QVILAITNPYPEIEPEDALAAGAAFAADGKSVNNVLGYPGIFRGALLSGAKQINPEMK
                     IAAAEILASLTTGDELVPDALDPNVHLQVAGAVREAAIASGAARLDRAPTGL"
     gene            complement(1625..2023)
                     /locus_tag="VFT03_01280"
     CDS             complement(1625..2023)
                     /locus_tag="VFT03_01280"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU4846801.1"
                     /translation="MARTQISNPNLPAPVRGGAYSSGVEAPAGRTVYVSGQVAFDAEG
                     NVVGEGDIKLQTETVLEHVKTVVEEAGGSMEDVVKVTVFITDMGLYDDIHEVRRRYFE
                     EPFPASSMVEVSALIDPRLLIEIEAIAVVR"
BASE COUNT          327 a          732 c          662 g          391 t
ORIGIN      
        1 gatcatgaag ctcttgccta tctctgtaca ccttcccgaa agggcgctgg cgccgtgcac
       61 tgctccttct cgcgcgacga tcagtcatac gcgagagcca gaggtttcgc atgccgcgcg
      121 agagacccta catgttgggg cggaggcctt ctgctaaagg cccgtaggcg cgcggtcgag
      181 gcgggcggct ccgctcgcta tggccgcttc acgcacggcg ccggcgacct gcaggtgcac
      241 gttggggtcg agggcgtcgg ggacgagctc gtccccggtg gtgagggagg cgagaatctc
      301 ggcggcggcg atcttcatct cggggttgat ctgcttcgcc cccgagagca gggcgccacg
      361 gaagataccg gggtagccga gtacgttgtt cacgctctta ccgtcggcgg cgaacgccgc
      421 ccccgccgcg agggcgtcct ctggctctat ctcggggtag gggttcgtga tcgccaggat
      481 cacctgcccc gcgcgcacca tctccggctt tatgaggccc ggcacgcccg tcgtcgcgac
      541 caccacgtcg gcctcgccca tgacggtctg catgtcggtg atctcgatgc ccttcgcgct
      601 agcgcgctcg tgcgagagcg ggttcgggtc agaggcgagc accctcttgg ccccggcgtc
      661 caccatgagg gcggctatcc cgaagcccgc ggcgcccagc ccgatctggc ctaccacctc
      721 ttcgcgcaaa tcgagcccgg cctggcggca cgcgaccagc gccgccgcga gcgtgaccac
      781 ggccgtgccg tggacgtcgt cgtgcatgac gggttggggg agcctctcga tgaggcgggc
      841 ctctatctcg aagcactccg gcgccgagat gtcctcaagg tgtattccgc cgaaggtcgg
      901 cgcgatgcgg accaccgtct cgacgaaggt gtccacgtcc ctggcgtcga ccagtatggg
      961 catcgccgag acgccggcga gctgctggta gaagacggcc ttgccctcca tcaccggcat
     1021 cgaggccaca ggcccgatat cccccagtcc cagcacccgc gttccgttcg tgcagatggc
     1081 gacgctgcgc ccgatcatgg tcagctcgct cgcgagcgag gggtcctcga cgatggcctc
     1141 gcacgcccgc gctaccccgg gcgtgtacac gtcgcgcatg tcctgaacgg tcctgaccgg
     1201 gtgggccgac tcgatcacga gcttcccgcc ctcgtgcttg aggagcgccc gatcacgcgc
     1261 ccacacgacc tcgacgcctt ccagagcgtt gagcatcccg acgatccgct cggcctggtc
     1321 gcggtcattg atctcgagcg tgatctcgcg cagcgaggac tcgcggccga cgttgatcgt
     1381 cgtcacgtct ccaatcaggc cgcctccatc ggctatcgct cctgcgaccc tcgcaaactg
     1441 gcccgtgcgg tgcgggatct ggatgcggta ggtgtaatcg agtggcatgg cggaccccct
     1501 aacgtccctg acagtccctg tcttggtgcc ctcgaacaag cgagagccaa actaccccac
     1561 gcccggattg tatagggtgc tcgtcccccg cgcgggtgtg cctcttcgct agactattcc
     1621 ggtgtcaccg gacgacggct atggcttcga tctctatgag caatctggga tctatgaggg
     1681 ctgagacctc gaccatcgag gaggcgggga agggttcctc gaagtagcgt cttctcacct
     1741 cgtgaatgtc gtcgtagagc cccatgtcgg taatgaagac ggtcaccttg acgacatcct
     1801 ccatactgcc tcccgcctcc tcgacgacgg tcttgacgtg ctcgagcacg gtctccgtct
     1861 gaagctttat gtccccctcg ccgaccacgt taccctcggc gtcgaacgcg acctgccccg
     1921 agacgtagac agtcctccca gccggagcct cgaccccgga cgagtaggcg cccccgcgca
     1981 ccggtgcagg caggttgggg ttcgagatct gggtacgtgc cataccgctc tcctctcctc
     2041 acgattttgc gacaccctag catcgcccgc ggtttcgcac ctgttgtaaa ctcgcccggg
     2101 gtaagcttca tg
//
LOCUS       DASTUV010000059         8153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1781017, whole genome shotgun sequence.
ACCESSION   DASTUV010000059 DASTUV010000000
VERSION     DASTUV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781017"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..168)
                     /locus_tag="VFT03_01285"
     tRNA            complement(96..168)
                     /locus_tag="VFT03_01285"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Lys,seq:ctt)
     gene            complement(513..1844)
                     /locus_tag="VFT03_01290"
     CDS             complement(513..1844)
                     /locus_tag="VFT03_01290"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846802.1"
                     /translation="MSGTVGEFFGGSGRRIRDVVWGDVPIDRAVRALLDTGAMERLKG
                     MSQLGFTFGSFPRARHTRFDHAIGVYYLTQMTLRRIAESGAYLETRDVQSAMAAALLL
                     DAARYPYSRAIEGIALPGAPTFKELSRRWIEHTEVAKVLRNEWDVEPHNVYRLVSRGS
                     DPPRSLTPTEHLLRDILFGSLDVDALDRLVRDARGAQVPYGTIKVEHLIEHLRIVGQE
                     NRAVLTIDEDGVGSLQAFVFSRYLMHYNVYEHRALRVPTVMFRRAVQDAVQAGTVTFD
                     KLSEQDDAGAFVLIQDKAEPESSTAHLMKRLSERKPYYPALEFDERHPSYASLIRLRD
                     DASWRRRVEEAWARYLTRYRKGVAGHFDILIDLPEGQRLPVDLRLIRRTPLASERNPV
                     SWQSISGMTEDDLQRYHAPLHRILIATADEELATSVRRHAEELFTIAEEVG"
     gene            1985..2479
                     /locus_tag="VFT03_01295"
     CDS             1985..2479
                     /locus_tag="VFT03_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4846803.1"
                     /translation="MTLRPPRESDVEAAYSWDCDPELAAWNGRSPISISLSAARRDYL
                     ARWQDPSVKTFIMEAGEPHEPIGMVTLYDFRNAGCELGIKIGAEDLRGKGYATEAVEL
                     LLAYAFDCLGLEVVRGSTLSHNERMRRVFEKNGFKQVGDGAILSRYDNRRYTELFYQR
                     RRED"
     gene            complement(2557..3663)
                     /locus_tag="VFT03_01300"
     CDS             complement(2557..3663)
                     /locus_tag="VFT03_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU4846804.1"
                     /translation="MAETRDNEMRVVRIVDLRHCLIEAFERVELPREDAEQVAEVLLD
                     SELRGHESHGVHLLGFLADAYRDGMNPRPRIRVLRETEGALLLEGDRSYVVGAIRGMS
                     WCIERARERKGMAVAGVRSSQPIVLGFYARMAAEAGLIGFACANAIPMVAPPGGRTPT
                     FGTNPFAYAIPAGRHPPVVLDVATTTGAAFKVRLAAQRNSPVPEGMILDREGRPTTDP
                     NEFVVGGLMAPLGSPAAPHKGFGLALVIDALAGVLTGAAFARDFPSEPATAGNATFWA
                     LDVEAFLPREEFLARMKAQIDHVKAGERLAGIDELFVPGERGHRRYHKLTTRGTVPLS
                     ATAWDSLTNVCASLSISPPPTLAPEPRRTDGGLA"
     gene            3931..4827
                     /locus_tag="VFT03_01305"
     CDS             3931..4827
                     /locus_tag="VFT03_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846805.1"
                     /translation="MNVRSRGAMSGVLTMGSLDPAFAGAVRVDGEPVVLDLRAVEFVE
                     PAGLCGLAALVEFLIPRSEEVGIALAGRDVPAYLERMDFFRLFGDRVRTNVDVASLEE
                     RRRHNPGTLQELINFHTEEEIPGIIERISEILENQGYRLRERVAICSVLSEICANAAE
                     HGASSFGAYAAVQAYHHIVSGPRRRGEEVLISIADGGPGVRETLSHNPALAEFTTTDN
                     DALRHALEMGVSGTGEIGRGGGLALVAGIAARSSGSLSLRSGTGRVTVYENRKNSRNV
                     PEFPGTFVRVSLPRLPQERSPI"
     gene            4924..5496
                     /locus_tag="VFT03_01310"
     CDS             4924..5496
                     /locus_tag="VFT03_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU4846806.1"
                     /translation="MPEERAHRIFEVAGGDFGGKLMVTRSTGRAVREALEETLTALPK
                     GGTLYVDTRGVELMDYSFADEALGILASRAASGEYGDRHLVLVEEDRDLLENVEASLR
                     QRSLAMIRVDEVGGEPGIVGEVPEHLVATLQAIYDAGSITNAALAAKLGLNHTACNNR
                     ATRLAKLGLIHRRTETAAPGGRQYSYERVV"
     gene            5576..6166
                     /locus_tag="VFT03_01315"
     CDS             5576..6166
                     /locus_tag="VFT03_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846807.1"
                     /translation="MREEYMIERQGKRFVLYAGLLEEAHSRGLRSIETELLQVPGPGN
                     GEVAIVKAVVRTEDGKFTGIGDASPENVNRTIVPHVIRMAETRAKARALRDAINVGVT
                     AFEELGGEDGAEIVEPTRGSEKQDERPAARVQENRGELPATKKQLNYLEALISDVVDD
                     DGGGMEKFEDMVGKPMGELTREEASEWISRLSGKAA"
     gene            6372..6644
                     /locus_tag="VFT03_01320"
     CDS             6372..6644
                     /locus_tag="VFT03_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846808.1"
                     /translation="MKGKRSSLRPDTTLAPVEEPEAEVYVWSLPGCCLRSLKEAALAE
                     AGERRRLELECSCGRTWRVTSSLDERILGRFVTHGGPRVGGSHPAA"
     gene            6700..7551
                     /locus_tag="VFT03_01325"
     CDS             6700..7551
                     /locus_tag="VFT03_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4846809.1"
                     /translation="MMQTQDVLTQYLGRVRNGTLLEAHEERDLSRRSHEGDTGARRRL
                     IESNLRLVISIAKKYRGRGMPFEDLIQEGNAGLIRAVEKFDPEMGNRFSTYATWWIRQ
                     AVTRAIADHARTVRLPAHVVDSLYRMRRAENALGIELGRDATEEELAERLGVKAEETR
                     RLREVSQPISSINARIGGAGDEDGSEMGDLLPDERSGDDYASVEVGQWERALVEAVRS
                     LPEREARVLEMRHGLDGGNVRTLREVSEVLGISQERARQVEIKALRTIRTGRYASTLQ
                     RALAEAV"
     gene            7713..>8153
                     /locus_tag="VFT03_01330"
     CDS             7713..>8153
                     /locus_tag="VFT03_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU4846810.1"
                     /translation="MEKTAPETQRAPELFETLGGIVADGFPAIHSAVYHTGRGTPYLR
                     SPGVVMLTRPRTNVAGLAGFLEGFDPELRFPEYLDDPTRLPDSTQLCKTAGQLCYASF
                     GPRRTTNENAAAYIGRLTSAGHGSVLEHASFSFLLYGISRSVTHE"
BASE COUNT         1452 a         2568 c         2750 g         1383 t
ORIGIN      
        1 ctattgctgc cgcattgctg ccaaagcccc cggtgctcca tcgggggctt cctagcgccg
       61 tcgcgtttta cctgcaaatc aaggcttttt ccgagtgagc cgcccaggac tcgaacctgg
      121 aacctagaga ttaagagtct cttgctctgc caattgagct agcggcccgc aaaaaacgat
      181 tatagcatcg ccgcgcgaag tgtcgaaagt taagttttgt ttatggggct tgcacgaatt
      241 ccgagcgcgc atttataatg ttcgtatcat cctagcggct ctttctgggg agccgctaag
      301 ggccggaaaa gggtctcacc cttatgtgaa caccggccct ttttgtttga gctagccagc
      361 acttcagcat ggcagcttct cgccgttcga cccttacccg gccttgcgtt ttgcgaggcg
      421 tcttactgac ggaagctttg ccaggcgccg cggagcgacg aaccgcgcta ctttgctgac
      481 aagccgccgt gctgactagc tgacatgcta actcagccca cctcctcggc gatggtgaag
      541 agttcttcgg cgtggcggcg cacggacgtc gcaagctctt cgtcggcggt ggctatgagg
      601 atgcggtgga ggggggcgtg gtagcgctgg aggtcgtcct cggtcatgcc cgagatgctc
      661 tgccagctca cggggttgcg ttcgctggcg aggggggtgc gtcggatcag gcgcagatct
      721 accgggagcc tctgtccttc ggggaggtct atgaggatgt caaagtggcc ggcgacgcct
      781 ttgcggtagc gggtcaggta gcgggcccag gcctcctcga cgcggcggcg ccaggaggca
      841 tcgtcgcgca ggcgtatgag cgaggcgtag ctcgggtggc gctcgtcgaa ctccagagcg
      901 gggtagtagg gcttgcgctc tgagagacgc ttcatcaggt gggccgtgga gctctcgggc
      961 tctgccttgt cctggatcag gacgaatgcc ccggcgtcgt cctgctcgga gagcttgtcg
     1021 aaggtcacgg tcccggcctg aacggcgtcc tggacggccc ggcggaacat cacggtcgga
     1081 acgcgtagcg cccggtgctc gtagacgttg tagtgcatca ggtagcggga gaagacgaac
     1141 gcctggagtg agcccacacc gtcctcgtct atagtaagga cagccctgtt ctcctggccg
     1201 acgatcctga ggtgctcgat caggtgctcg accttgatgg tgccgtaggg tacctgcgcc
     1261 cctctggcgt cgcgcacgag cctatcgagg gcgtccacgt cgagcgagcc aaagaggatg
     1321 tcgcgcagca ggtgctcggt cggggtgagg ctgcgcggcg gatcgctgcc ccgcgagacg
     1381 aggcggtaga cgttgtgggg ctcgacgtcc cactcgttcc tcaggacctt cgccacctct
     1441 gtgtgctcga tccagcgccg gctcaactct ttgaaggtcg gcgcgccagg caaggctatc
     1501 ccctcgattg cgcgcgagta agggtaccga gccgcgtcaa ggagcagggc ggcagccatg
     1561 gcgctctgca cgtccctggt ctcgaggtag gcgccggatt ccgcgatcct cctcagtgtc
     1621 atctgcgtca ggtagtagac gccgatggca tggtcgaagc gcgtgtgcct ggccctcggg
     1681 aaggacccga aggtgaaccc gagctggctc atgcctttga gacgctccat cgcgcctgtg
     1741 tcgagcagcg ccctcaccgc ccggtctatg gggacatctc cccagactac gtcccggatt
     1801 cttcgcccgg agcccccgaa gaactcccct acggtcccgc tcaatgccgc tcccgccatt
     1861 cgcttcgggg tattctagcg aaaatgctgg aagccgacgc tcgagacctg tgtaatatag
     1921 agggtcgcga aggagaggag ccgccataca cgccgaagag gtcgaggttc tgcgcgggaa
     1981 gtgcgtgacc ctgaggccgc cgcgcgagtc ggacgtggag gcggcatact cctgggactg
     2041 tgaccccgag ctcgccgcgt ggaacgggcg ctcccccatt tccatctccc tctccgccgc
     2101 ccgccgcgac tacctggccc gctggcagga cccgagcgtg aaaaccttca ttatggaggc
     2161 cggggagccc cacgagccca tcggaatggt caccctctac gacttccgca acgcaggctg
     2221 tgagctcggc ataaagatcg gcgccgaaga cctgcgcggt aagggctacg cgaccgaggc
     2281 agtcgagctg ctcctcgcct acgccttcga ctgccttggc ctcgaggtcg tgcgcggctc
     2341 caccctctcc cacaacgaac ggatgcggcg cgtcttcgag aagaacggct tcaaacaggt
     2401 cggcgacggc gctatcctca gccgctacga taaccgccgc tataccgagc tcttctacca
     2461 gcgccggcgc gaagactagc cctctgtcaa gcctgaaatg actgatacgc ctccagccgg
     2521 tagcgcgctg gaggcgtcgc cagttcttgc gatgctctag gccaatccac cgtctgtccg
     2581 ccgcggctca ggcgcaaggg tgggcggagg tgagatagac agggatgcgc aaacgttcgt
     2641 gagcgaatcc caagccgtcg ccgagagcgg gaccgtgccg cgggtagtta gcttgtgata
     2701 acggcggtgg ccacgttcgc ccggcacgaa cagttcatcg atgcctgcca gccgttcgcc
     2761 ggccttgacg tggtctatct gggccttcat ccgcgccagg aactcctcgc gcggtagaaa
     2821 cgcctccacg tcgagcgccc agaaagtggc gttgccggct gtggcgggtt cgctgggaaa
     2881 gtcgcgggca aaagcggcgc cggtcaagac gccggccaga gcgtcgatga ccagcgccag
     2941 accgaagccc ttgtgaggtg ctgccgggga accgagcggt gccatgagcc cgcccacgac
     3001 aaactcgttc gggtcggtgg tcgggcggcc ttcacggtcg aggatcatgc cctcgggcac
     3061 cggactgttg cgttgtgccg cgagccggac cttgaacgcg gcgccggtgg tcgtggccac
     3121 gtcgagcacc accggtgggt ggcggccggc cgggatggca taggcgaacg ggttggtgcc
     3181 aaaggttggc gtgcggccgc cgggcggggc gaccatcggg atagcattgg cgcaggcgaa
     3241 gccgatcaag ccggcctcgg ccgccattcg ggcgtagaat ccgaggacga tgggctgtga
     3301 gctgcggacg cccgcgaccg ccatgccttt gcgctcacgg gcacgctcga tgcaccagct
     3361 catcccccgt atcgccccga ccacgtagct gcggtccccc tcgaggagca gcgctccctc
     3421 cgtctctcgc agcacccgga tgcggggccg cgggttcatg ccatcacggt acgcatcggc
     3481 caggaagccg agtaggtgta cgccgtggct ctcgtggccg cgcagttccg agtccagcaa
     3541 gacctcagca acctgttcgg catcctcgcg cggcagctcg acccgctcga acgcctcgat
     3601 gaggcaatgc cgcaaatcta cgatccgaac cacgcgcatc tcgttgtccc gagtttcagc
     3661 catgaccagc cttccccttt caccgaggca ggattgcgtg catcgtagca acctctccgc
     3721 gctcgggcac gtatgcccgc agggatggtc aactggcagt tcgcgaccaa cgacgtgagg
     3781 atcgagtgca agtgggtgta tccatcagtt cagggccgac aaagctctgg ccacgccctg
     3841 tccagacccc attttctact gcgatagatc ttgctatccg ctttctggtc ctttccggct
     3901 acaataaggt cagtgagaac gcagttacag ttgaacgtgc ggagccgggg cgctatgagc
     3961 ggggtgctca ctatgggcag tctcgacccg gcgttcgcgg gggcggtacg cgttgacggg
     4021 gagcctgtgg tcctggacct gcgggcggtc gaattcgtgg agccggccgg gctctgcggc
     4081 ctggcggcgc tggtggagtt cctgatcccc cgctccgagg aagtgggtat cgccctcgcg
     4141 ggacgcgacg tgccggccta cctggagcgg atggacttct tcaggctctt cggagacagg
     4201 gtgcgcacga acgtagacgt ggcctctctc gaagagcgcc gccgacacaa cccggggacc
     4261 ttgcaggagc tcatcaactt ccacacggaa gaggagatcc ccgggatcat cgagcgtatc
     4321 tcggagatcc tggagaacca gggttaccgc cttagagaga gggtcgccat ctgctcggtt
     4381 ctctcggaga tctgcgcgaa cgccgccgag cacggcgcct cctcgttcgg cgcctacgcc
     4441 gctgtccagg cctaccacca catcgttagc ggcccccgtc ggcgcggcga agaggttctg
     4501 atctccatag ccgacggcgg ccccggagta cgcgagaccc tctcccacaa ccccgccctc
     4561 gccgaattca ccaccacgga taacgacgcg ctgcgccacg ccctcgagat gggcgtctcc
     4621 ggtacggggg agatcggtcg cggcggcggc ctcgctctcg tcgcaggcat cgccgctcgc
     4681 tctagcggct ccctctcttt gcgttccgga accggccgcg taaccgtcta cgagaaccgc
     4741 aagaactccc gtaacgtccc cgagttcccc ggtaccttcg tccgcgtctc cctgcctcgc
     4801 ctcccacagg agagatcccc gatttgaggg cgaaaattac cccccgaata ccattgattt
     4861 acagccccac agggagtaca attcagccag atgatttcac tgacgacagt gaggacggga
     4921 tcggtgcctg aggagcgggc gcaccggatc ttcgaggtgg ctggcggcga cttcgggggc
     4981 aagctgatgg tgacccgttc gacgggccgg gcggtccggg aggcgctcga ggagacgcta
     5041 actgcgttgc ccaagggtgg gacgttgtac gtggacaccc ggggcgtcga gttgatggac
     5101 tactcgttcg cggacgaggc gttgggaatc ctggcgtcga gggctgcgag cggggagtac
     5161 ggggaccggc acctggtgct cgtggaggag gaccgggacc tgctggagaa cgtcgaggcc
     5221 tctttgaggc agcgttcgct ggccatgatc cgggtggacg aggtcggcgg ggagccgggc
     5281 atcgtcggcg aggtgcccga gcacctggtc gcgaccttgc aggcgatcta cgacgcaggc
     5341 tccatcacga acgcggcgtt ggcagccaaa ctcggcctca accataccgc ctgcaacaac
     5401 cgcgccacca ggctcgcgaa gctcggcctg atccaccgcc gcactgagac agccgccccc
     5461 ggcggacgcc agtattccta cgaaagagtc gtctagtgtt ttgttttgac catatcctca
     5521 ctcatttcag tgaagacagc ggtaccggtg taggagcgcc ggaggaggta tagcggtgcg
     5581 agaagagtac atgatcgaac gccagggcaa gaggttcgtg ctgtatgcgg gtcttttgga
     5641 ggaggcgcac agcaggggcc tgaggagcat cgagacggag ctgctgcagg ttccgggccc
     5701 gggtaacggc gaggtcgcca tagtcaaggc tgtggtgcgc acggaggatg gcaagttcac
     5761 gggtataggg gacgcgagcc ccgagaacgt gaaccggacg atagttccgc acgtgatccg
     5821 gatggccgag acgcgggcga aggcccgtgc cttgagggat gcgatcaacg tcggcgtcac
     5881 cgcattcgag gagctcggcg gcgaggacgg cgcggagatc gtcgagccta cgcggggctc
     5941 tgaaaagcag gatgagcgtc cggccgcccg cgtgcaggag aacaggggcg agcttccggc
     6001 aaccaagaag cagctcaact acctggaggc tctcatctcc gacgtcgtcg acgacgacgg
     6061 gggcgggatg gagaagttcg aggacatggt tggcaagccc atgggcgagc tcacccgcga
     6121 ggaggcgagc gagtggatca gccgcctctc agggaaggcc gcgtagtgcg aacctggatc
     6181 gcagcccttg gcgaggatgt cgtcctctac gaggtggacc ttcccgcctg ttgccggcgg
     6241 agtgctgtgg gctcgggcca ttccttcgaa tgcccctcgt gcggagcggt atggcaggcg
     6301 ccgattcccg tcgaggccga tgaggacgct tttgggcagc gcgacacgga ggagcggaaa
     6361 ggggccgctt gatgaagggg aagaggagct ccctcagacc agacacgacg ctagcgccgg
     6421 tcgaagagcc ggaggccgaa gtctacgtct ggtctctgcc tggctgttgc ctgcggtcgc
     6481 tcaaggaggc ggccctggcc gaggcggggg agaggcggcg gctcgagctc gagtgctcgt
     6541 gcgggaggac ctggcgcgta acctcgagcc tggacgagag gatcctggga cggttcgtga
     6601 cacacggcgg cccgcgggtc gggggttcgc accccgcagc gtgatgaaaa gctcttcagc
     6661 caggaacacg gaagctgaaa tgctgacaag ggagacggga tgatgcagac gcaggacgtt
     6721 ctcacgcagt acctgggcag agtccgaaac ggcacgctac tcgaagctca cgaggagcgg
     6781 gacctcagca ggcgctccca tgagggcgac acaggggcca ggcggcggct catcgagtca
     6841 aatctgcggc tcgtgatctc gatcgccaag aagtaccgcg ggcgcggcat gcccttcgag
     6901 gacctcatcc aggaggggaa cgcgggcttg atacgggcag tcgagaagtt cgaccccgag
     6961 atgggcaacc gcttctcgac ctacgcgacg tggtggataa ggcaggccgt aacccgcgcc
     7021 atagccgacc acgcccgcac cgtccgcctc ccggcccacg tcgtcgactc cctgtaccgt
     7081 atgcggcggg ccgagaacgc ccttgggatc gagctcggtc gtgacgctac ggaggaggag
     7141 ctggccgagc gactcggcgt caaggccgag gagacgcggc gcctgcgcga ggtcagccag
     7201 ccgatctcga gcatcaacgc gcgcatcggc ggggcaggag atgaggacgg ctccgagatg
     7261 ggtgacctcc tgccggacga gaggagcggc gacgactacg ccagcgtcga ggttggccag
     7321 tgggagcggg ctctcgtcga ggccgtcagg tcgctgcccg agcgcgaggc cagagtcctc
     7381 gagatgcgcc acggcctgga tggagggaat gtccggaccc tgcgcgaggt ctccgaggta
     7441 cttgggatct cgcaggagag ggcccggcag gtagagatca aagccctcag gaccatccgc
     7501 accggccgct acgccagcac actccagagg gcgctcgccg aggccgtcta gactggcggg
     7561 ttggccgcat ccgagtccgc taatcgggcc gtcgaggcac acgcctcgac ggcctctttc
     7621 gtaccgctac gagatcgcct ctcgagcagc cgaggtggag aattctcggc gcaagagcta
     7681 ccctgcgtag agagcgagaa gaagggagcc ccttggagaa gaccgcacct gaaacgcagc
     7741 gcgcaccaga gctgttcgag acgttgggag ggatcgtcgc cgacgggttc cccgcgatcc
     7801 actccgccgt ttaccacacc ggccgcggca caccctatct ccggtcccct ggggtcgtaa
     7861 tgctgactcg gcccaggacc aacgtggccg ggctggccgg cttcctcgag ggattcgacc
     7921 ctgagctccg gttccccgaa tacctcgacg atccgacccg gctgcccgac tctacccagc
     7981 tctgcaagac cgctggacaa ctctgttacg ctagcttcgg tccccggcgc acgacgaacg
     8041 agaacgccgc cgcctacatc gggcgtctga cctcggcggg gcacggctcg gtactcgagc
     8101 acgcgagctt cagcttcctg ctctacggca tcagccgcag cgtcacccac gag
//
LOCUS       DASTUV010000060         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_671743, whole genome shotgun sequence.
ACCESSION   DASTUV010000060 DASTUV010000000
VERSION     DASTUV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671743"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFT03_01335"
     CDS             <1..614
                     /locus_tag="VFT03_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4846811.1"
                     /translation="GRIPSIIVTLGMLYALRGVILLVTGGTWITGIPESTRFMGTGKL
                     LGISVPVLILFGLFLVMELVNRHATWGRNVFAVGGNRRAARFAGLPVNRVRFMAFALV
                     GVFVGIASLIYLGRAGSVQTNTGVGLELQVVAAVVIGGTSIAGGRGSSLAALTGAVLI
                     GVILNGLILLGVPGIWQDAVLGALILLAVATDVLRRRIIGDKV"
     gene            611..1567
                     /locus_tag="VFT03_01340"
     CDS             611..1567
                     /locus_tag="VFT03_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4846812.1"
                     /translation="MSALTEGVRRLGFSREIVLLGVLIVLVIVMSLLDPLFLSLTTLL
                     NTSRFFVEVGLMALGMTLIIITGGIDLSVGSNVALVSVAVGFSFAAGLPLPLAIVFGL
                     MVGVAAGLFNGLFITLLDLHPLVVTLGTLALFQGLAYGISKAEAVSDFPGWFAYFGQA
                     YLGPVPGQLFIFVVAVAVVWVILSRTRYGRYVYAIGNNEEAARFSGVPIRRVKLALYS
                     GIGLLVGMAAVIYTSRVYTARGDSGVGLELDVISAVVLGGASIYGGSGTILGTVLGVL
                     IIAVLRNGLILAGVPSTWQLFVLGVLLLAAVFLNEFFRRGEE"
     gene            1627..2643
                     /locus_tag="VFT03_01345"
     CDS             1627..2643
                     /locus_tag="VFT03_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4846813.1"
                     /translation="MIRFARVAVVSVLVIAAAACGVGQQGPSQEEGGGGGGPNKICMM
                     PKLVGIPYFNASEQGAKEAAEELGVDLVYDGPTEAKAALQSQMIEQFVQQQCDAITVA
                     ANDPDALAPAMKKAGDAGIATGAWDADVAPDAREVFVNQATFEAIGYELVDVMAKQTG
                     GKGEFLVVTGSLTAPNQVAWLKEMRERMKEKYPEMSIASVEPGEEDLQLGIDITKNYL
                     RAHPDTSGVFGITSVALPGAAEAVEQMGRAGEVPVTGLSTPNEMKPHLESGAVKEFVL
                     WNPVDLGYLAVYVANAQVDDKLPSSGTFKAGRLGEVKMLSEDEVLLGPPIVFSKENID
                     QYDF"
BASE COUNT          423 a          873 c          889 g          513 t
ORIGIN      
        1 tggggcgcat accttccatc atcgtgacgc tcgggatgct ctacgcgctg cggggcgtca
       61 tactgctcgt cacagggggg acctggatca ccggcatccc cgagagcacc cgatttatgg
      121 gcacggggaa gctgctaggc atcagcgtcc ccgtcctaat cctcttcggc ctgttcctgg
      181 tgatggagct ggtcaaccgc cacgccacct ggggtcgcaa cgtattcgcc gtcggtggca
      241 accggcgggc cgcccgcttc gcggggttgc cggtgaaccg cgtacggttt atggccttcg
      301 cgctggtcgg ggtgttcgtc ggcatcgcct cgctcatcta tctcgggcgg gccggttcgg
      361 tgcagaccaa cacgggggtc gggctcgagc tgcaggtggt cgctgccgtg gtcatagggg
      421 ggactagcat cgccggaggc aggggctctt ctctcgccgc gctcacgggg gccgtcttga
      481 taggcgtcat cctgaacggc ttgatcctgc tcggcgtccc cggcatctgg caggacgccg
      541 tgctcggagc cctgatcctg ctcgccgtcg caaccgacgt gctgcgcagg cgcatcatag
      601 gagacaaggt atgagcgcgc tcacggaagg cgtaaggcgc ctcgggttct cccgagagat
      661 agtgctgctg ggggtcctga tagtcctagt tatcgtgatg tccttgctgg atcccctgtt
      721 cctttccctg accacccttc tcaatacctc gcgcttcttc gtcgaagtgg ggctcatggc
      781 gctcgggatg acgctcatca tcatcaccgg cggcatagac ctctccgtgg ggtccaacgt
      841 agccctcgtc tcggtggccg tcgggttctc cttcgccgcc ggcctgcccc tcccactggc
      901 catcgtgttc ggcctcatgg tcggcgtggc cgccggcctc ttcaacggtc tgttcatcac
      961 cttgttggac ctgcacccgc tcgtagttac tctgggcacc ctggccctct tccagggcct
     1021 ggcatacggc atctcgaagg ccgaggcggt ctccgacttc cctggctggt tcgcgtactt
     1081 cggccaggct tacctggggc ccgtgccggg acagctcttc atcttcgtcg tcgccgtggc
     1141 ggtggtgtgg gtgatcctct ctcgcacccg ctacggccgc tacgtctacg ccatcgggaa
     1201 caacgaggag gcggcccgct tctccggcgt gcccatccgc agggtcaagc tggccctcta
     1261 ctcgggcatc gggttgctgg tagggatggc cgcggtgatc tatacctcgc gggtctacac
     1321 ggcgagaggc gactccggtg tcgggctgga gcttgacgtg atctccgccg tggtccttgg
     1381 cggcgcgagc atctacggcg gctcagggac gatactcggc acggtgctcg gcgtcctgat
     1441 catcgcggtt ttgcgcaacg ggctgatact cgccggcgtc ccgagcacct ggcagctctt
     1501 cgtgctcggc gtcctgctcc tcgcggccgt tttcctgaac gagttcttcc gaagggggga
     1561 ggaatagtac atgccgggat gacgggtgga tctgtagcga aggaaagctc tgggaaagga
     1621 ggcacaatga tacgttttgc gcgggtggct gtggtgtccg tgctggtcat cgccgccgcc
     1681 gcttgcgggg tgggccagca gggcccgtcc caggaagaag gaggcggtgg aggagggccg
     1741 aacaagatct gcatgatgcc caaactggtc ggcatcccgt acttcaacgc ctccgagcag
     1801 ggggccaagg aggcggccga ggagcttggt gtcgatctgg tgtacgacgg tcccaccgag
     1861 gccaaagccg cgcttcagtc gcagatgatc gagcagttcg tccagcagca gtgcgacgcc
     1921 attacggtcg ccgcgaacga cccggacgcc ctcgctcccg cgatgaagaa ggccggcgat
     1981 gccgggatcg ccacgggcgc ctgggacgcc gacgtggcac ccgacgcgcg cgaagtcttc
     2041 gtcaaccagg caaccttcga ggccataggc tacgagctgg tggacgtcat ggccaagcag
     2101 accggcggca agggcgagtt cctggtcgtc acaggctcgc tcaccgctcc caaccaggtg
     2161 gcctggctca aggagatgcg cgagcgcatg aaggagaagt accctgagat gtccatcgcc
     2221 tccgtcgagc ccggcgagga ggacctccag ctcggtatcg acataaccaa gaactacctg
     2281 cgggcccacc ccgacacctc cggcgtcttc ggcataacct ccgtggccct gcccggcgca
     2341 gccgaagccg tcgagcagat gggccgcgcg ggcgaggtgc ccgtcaccgg actctccacc
     2401 cctaacgaga tgaagccgca tctcgagtca ggcgccgtaa aggagttcgt cctctggaac
     2461 ccggtggatc tcggctacct cgcggtgtac gtcgccaacg cccaggtcga cgacaagctt
     2521 cctagctcgg gtaccttcaa ggccggccgt ctcggcgagg tcaagatgct ctccgaagac
     2581 gaggttttgc tcggcccgcc aatcgtgttc tcgaaggaga acatcgacca gtacgacttc
     2641 tagagagcga taccagcgcc ggcgcctctt aagcgagagg cgccggcgtg gggcccgg
//
LOCUS       DASTUV010000061         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_636948, whole genome shotgun sequence.
ACCESSION   DASTUV010000061 DASTUV010000000
VERSION     DASTUV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636948"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFT03_01350"
     CDS             complement(<1..294)
                     /locus_tag="VFT03_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU4846814.1"
                     /translation="MDFLEVLKRRKTTNGPFLPDPVKPEHQRLLVEVAAMAPSHFNSQ
                     PWRFVLVDDRETIDEVARISGESMKRLLEEGTFWKRYRPYFRFTEAEMEEKRDG"
     gene            complement(305..613)
                     /locus_tag="VFT03_01355"
     CDS             complement(305..613)
                     /locus_tag="VFT03_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846815.1"
                     /translation="MIFYIAMVLFVANFALGVLVQFRIVDTKPFRWLHHTLFFAVFAS
                     ATLAVGVGFLQGAPYRWVLLPVLILFAFLPRVRAGTPGHAALASGAMVFYVSGFVWML
                     "
     gene            736..1977
                     /locus_tag="VFT03_01360"
     CDS             736..1977
                     /locus_tag="VFT03_01360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU4846816.1"
                     /translation="MANRRPEAYITGIGAVTPIGVGRKNLWSSLLANESGAGPITLFD
                     PQDFEVRIAAECTDFDPESFMDRKVAARTDRFGQMGLASAKLALEDAGAWDVLNNNPE
                     RVGLILGSGIGGVTSMETTQATIDSKGPSRVNPFAVTKIMPNSAAAHVGIQLGVQGPS
                     ITGALACTCGTDMMGLGYDLIRRGDSDVVLCGASEAGLAPVIVAGFIAMRAMSRRNDD
                     PEGACRPYDVDHSGFLIGEASAVVVLESAESVERRGSEPYAKITGVGRTTDAYNLTDP
                     DPKGFGIMRAMQLAVADSGLRGEQIGYINPHGTGTVAGDGPESQAMAKINPNVKVSAT
                     KSTLGHSLGASGAVETAICALAIKERKIPPMRNLTQVADGCADLDYVVDEPREAPDLE
                     AALCANLGVGGHNAAVVLERA"
     gene            1986..>2224
                     /locus_tag="VFT03_01365"
     CDS             1986..>2224
                     /locus_tag="VFT03_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU4846817.1"
                     /translation="MDRPENGYEVPQGLPEPEDNGACDHLPGMRMPSVSLTSTTGDPV
                     DISTLTGTNVLYCYPLTGRPDQDLPPGWDDIPGARG"
BASE COUNT          460 a          712 c          712 g          340 t
ORIGIN      
        1 gccatcgcgc ttctcctcca tctcggcctc agtgaaacgg aagtagggcc tgtagcgctt
       61 ccagaacgtg ccctcctcca gaaggcgctt catgctctcg ccgcttatcc tcgcaacctc
      121 atctatagtc tcgcggtcgt ctaccagcac gaagcgccag ggctggctgt tgaagtggga
      181 aggtgccatc gcggcgacct cgacgagcag gcgctggtgc tcgggcttca cagggtctgg
      241 cagaaaaggg ccgttcgtgg tcttgcgcct cttgaggacc tccaggaaat ccaatctcag
      301 cctcctaaag catccatacg aatcctgaaa cgtagaagac catggccccg ctggctagcg
      361 cagcgtgccc cggcgttccg gcccgtaccc gcggcaggaa ggcgaagaga ataaggacag
      421 gtaatagaac ccagcggtag ggcgctccct gcaggaatcc gacgccaacc gctagggtgg
      481 cggaggcgaa gaccgcgaaa aacagggtgt gatggagcca gcgaaagggt ttcgtatcca
      541 ctatgcgaaa ctgcacgagt acgccgagcg cgaagttcgc aacgaagagg accatcgcga
      601 tgtagaagat caatgctcgc tccgccgaat catcctggag ggttatagca cagggcgggt
      661 cgcgccggag tcgacggggg cctgtgatgc taagattcat agttcgatgg atcttggatt
      721 ttgctagggg gcgagatggc gaataggcgc ccggaggctt acattacggg gatcggcgcg
      781 gtcaccccaa taggagtagg tcgcaagaac ctctggagct cgctcctggc caacgagagc
      841 ggggcaggcc ccataacgct cttcgatccc caggacttcg aggtgcgtat cgccgccgag
      901 tgcacggact tcgaccccga gagctttatg gaccgcaaag tggcggccag gaccgaccgg
      961 ttcgggcaga tgggcctcgc ctcggccaag ctggccctcg aggacgcggg cgcctgggac
     1021 gtgctcaaca acaacccgga acgggtcggc ctaatcctcg gcagcggcat cgggggcgtt
     1081 accagcatgg agacgaccca ggcgaccata gatagcaaag gaccctcccg cgtcaacccc
     1141 ttcgccgtaa ccaagatcat gcccaactcc gccgcggccc acgtaggcat acagctcggc
     1201 gtccagggac cctcgatcac cggcgcgcta gcctgcacct gcggcacgga catgatgggc
     1261 ctgggctacg acctgatccg gcgcggtgac tccgacgtcg tgctctgcgg cgcctcggag
     1321 gccggcctgg cacccgttat cgtggcagga ttcatagcga tgcgggcgat gagccgccgt
     1381 aacgacgacc ccgagggggc gtgcagaccc tacgacgtcg accactcggg gttcctgatc
     1441 ggcgaggcgt cggccgtggt ggtcctggag agcgccgagt ctgtagagcg ccgcgggtcc
     1501 gagccgtacg cgaaaatcac cggcgtgggc cgcaccaccg acgcgtacaa cctcaccgac
     1561 ccagatccga aaggcttcgg catcatgcgg gccatgcagc tcgccgtcgc cgactctgga
     1621 ctgagagggg agcagatcgg ctacataaac ccgcacggca cggggactgt ggccggtgac
     1681 ggccccgaat cgcaggccat ggccaagatc aaccccaacg tcaaggtctc ggccacgaaa
     1741 tcgaccctgg gccactcttt aggcgcgagc ggcgccgtgg agaccgcgat ctgcgccctc
     1801 gccatcaagg agagaaaaat ccccccgatg aggaacctta cgcaagtagc ggacggctgc
     1861 gccgacctcg actacgtcgt ggacgagccg cgcgaggcgc ccgacctgga agcggccctc
     1921 tgcgccaacc taggcgtcgg cggccacaac gcagcggtcg tcctggaacg agcctgagga
     1981 gtcgcatgga cagaccagag aacggatacg aggtaccaca gggcctaccc gagccggaag
     2041 acaacggtgc ctgcgaccac ctgccaggca tgaggatgcc ttccgtatcc ctgacatcga
     2101 cgacgggaga ccctgtggat atctccaccc tgacggggac gaacgtactt tactgctacc
     2161 cgcttacggg gagacctgac caggacctgc cacctgggtg ggacgacatc cccggcgctc
     2221 gtgg
//
LOCUS       DASTUV010000062         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_27954, whole genome shotgun sequence.
ACCESSION   DASTUV010000062 DASTUV010000000
VERSION     DASTUV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27954"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFT03_01370"
     CDS             complement(<1..844)
                     /locus_tag="VFT03_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4846818.1"
                     /translation="MDTIFYAALTPLVPLFEEEFGLSKSAVGILSGAYGAGVLVGSVP
                     GGYLASRSGVRITALVGLILMSVTSVMFALVDTTWLLVATRFIGGFGSALSWVAAFTW
                     LVDRAPEGRRAEMIGVMISAAVVGALLGPVLGSVAATLGLLPAFASVAIVGGVIAVWA
                     ALERAPASSGDISLLPALRTVREPLLLASLWFIVLSPLLFSALAVLVPLDLNRLGWSA
                     AAVGTVFFVSAVFEAFVHPLLGRWSDRSGYRPPVFAGLVASIAILLLLTWVGNPWVIG
                     LLVVL"
     gene            988..1305
                     /locus_tag="VFT03_01375"
     CDS             988..1305
                     /locus_tag="VFT03_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846819.1"
                     /translation="MTDWLAWKRRGPANRVLFVLSLSLAAFVTLTFAGASLYPDPGNF
                     ARYFAFYWVWGLCAIGVLLGMAEYKTSPLRKGKERGHLFILLYMGALLIVTRALNMVL
                     YAG"
     gene            1310..1951
                     /locus_tag="VFT03_01380"
     CDS             1310..1951
                     /locus_tag="VFT03_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU4846820.1"
                     /translation="MYRALLFDLDGTLAETDSLHLPTWVDALEPYGVEVDEEFYRDRI
                     SGRSTAEIVRELLPELTDEQGRSIGDAKEASFRKRSFELDPLPGLEDFIERGRKRGMW
                     IALVTNAPVENVEAILLALNLRDYFDTVVLADEVEAVKPDPAPYIAALEKTGVPAEEA
                     LAFEDSISGISSSVAAGIPTVGIASSQEPAKLLGAGAFMVAQDFTDPQLRALI"
BASE COUNT          419 a          659 c          624 g          331 t
ORIGIN      
        1 cgagcacgac gagaagtccg atcacccacg ggttcccaac ccaagtcagg aggagcagga
       61 tggcgatgga ggcgaccaat ccggcgaaga cgggcgggcg gtagccagag cggtcggacc
      121 agcggccgag cagcggatgg acgaacgctt cgaacacggc gcttacgaaa aagaccgtcc
      181 ccaccgccgc agcactccat ccgagcctgt tgagatccag cggtaccagg acggccagcg
      241 cgctgaagag caggggggag agcactataa accagaggct ggcaaggagc aggggctctc
      301 gtaccgtcct gagcgccggt aacagggaga tatcaccgga ggaagccggt gcccgttcca
      361 gggcggccca gacggcgatg actccaccca ctatggcgac ggaagcgaaa gccggcaaga
      421 ggccgagagt cgctgccacg ctgcctagta caggtccgag tagagccccc accacggctg
      481 cgctgatcat caccccgatc atctcggccc gccgcccctc cggagcccgg tcaacgagcc
      541 aggtgaacgc cgcgacccat gagagcgcgc taccgaaccc gccgatgaac cgcgtcgcca
      601 ctagcagcca agtcgtgtcc acaagcgcga acatcacgct ggttacggac ataaggatca
      661 atccgacgag ggccgtaatc ctcactcctg accgcgaggc aaggtatcca ccaggcacgc
      721 ttcctacaag gacgcctgcc ccgtaggccc cgctcaagat gccgaccgcg gacttggaga
      781 ggccgaactc ctcttcgaag agtgggacga gcggcgtaag cgcggcgtag aagatggtat
      841 ccaccaacac tatcgcgcag ctgagggcaa ggagtcggcg cacgacggtt attctaatgt
      901 ctgggtacag cgcgtgagcc agaacctttc ccggcgcccc acgttctggc tgctaacatg
      961 actgatatga gcagtgtcca taccatcttg accgactggc tagcatggaa gcgtagggga
     1021 ccggcgaata gggtcctgtt cgtcctgtcg ctctctctgg ccgctttcgt gacgctaacc
     1081 ttcgcggggg cttcgctcta ccctgacccc ggcaacttcg cgcgctactt cgcgttctac
     1141 tgggtgtggg gtctctgcgc catcggtgtg ctcctcggga tggccgagta taagaccagt
     1201 cccttgcgca agggcaaaga acgtggccat ctcttcatac tcttgtacat gggcgcgctg
     1261 cttatcgtta cccgcgctct caacatggtc ctctacgccg gttagcggtt tgtaccgggc
     1321 cctgctcttc gacctcgatg gcacgctcgc ggagaccgac tccctgcacc tgccaacgtg
     1381 ggtggatgcg ctcgaaccat acggcgtcga agtagacgaa gagttctatc gcgacaggat
     1441 cagcggccgg agcacggccg agatcgtgcg cgaactgctc cccgagctca ccgacgaaca
     1501 agggcggtcg atcggtgacg cgaaagaggc gagcttccgc aagcgatcgt tcgagctgga
     1561 ccccctgcca ggtctcgagg actttataga gcgaggcagg aagcgaggga tgtggatcgc
     1621 cctcgtcacc aacgcgcccg tggagaacgt cgaggcgata ctgctcgccc tgaaccttcg
     1681 ggactacttc gacaccgtcg tgctcgccga cgaggttgag gctgtgaagc cggatccagc
     1741 cccctacata gccgccctgg agaagaccgg cgtccctgcc gaagaagccc tggccttcga
     1801 ggactcgatc tcgggcatat cctcctccgt cgccgcgggc atcccgaccg taggtatcgc
     1861 ctcgagccag gagcctgcga agctcctcgg ggcaggggcc tttatggtgg cacaggactt
     1921 caccgacccg caactgagag ccctcatcta gcgaagttcg ctgtaagggc ggtttacgat
     1981 ccgcccactc tcagcgggat gcgtccaaga acgcccgcaa cctctgcgca tac
//
LOCUS       DASTUV010000063         5480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1494387, whole genome shotgun sequence.
ACCESSION   DASTUV010000063 DASTUV010000000
VERSION     DASTUV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494387"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFT03_01385"
     CDS             complement(<1..183)
                     /locus_tag="VFT03_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846821.1"
                     /translation="MTPVDRKLLASSLSSDGQMSLPAGAYADVSVFRWELEHFFDESW
                     VCVGRAEDLRDAGDRRA"
     gene            593..1771
                     /locus_tag="VFT03_01390"
     CDS             593..1771
                     /locus_tag="VFT03_01390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4846822.1"
                     /translation="MPVYDVAIVGGGNLGLWTAYRLARRGFGRVAVLERGWAGGGATS
                     RSAGVVRQQGGSGTAATLGQLARELYLELGEELGLDPGFVETGYYIVAETEEEKESFQ
                     RLVEVRREAGVENEWVEPEEGKERFPDLDWDRFVGATYTSTDGYVNPQIAARNATCAV
                     LSSDSVDLFEMCEVRSIEERSGRYALETVCGTFEAERVVDAGGPRGAREVGAMVGVEV
                     PVMAVRHQIVTFPTIAEGAAHPFPLFFLVGKGYYARPEEQGALLGMSNASDEADPSER
                     YQIDFDWGYFEKTRPDWESAYPPLKGLPVSRAWAASIDYTPDHLPIIDEPMEGFYVLA
                     AGGHGMMWGPALGEKMAGLISEGSVSGVPREDVELARFSRVKDQDRVEDMISLPFPKK
                     "
     gene            1792..2958
                     /locus_tag="VFT03_01395"
     CDS             1792..2958
                     /locus_tag="VFT03_01395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4846823.1"
                     /translation="MAERPTIPEHVRLLKARIARFVREELHPFEAEIARRGEIDEEKL
                     GALREKARAAGFSNLNMPEEYGGMDLPVVSQVALEEEAGKTTNGLGYMVADRGPRELL
                     DLASPEQVERYVMPVIRGETREAWAITEPGAGSDIKDLATTAERDGEGWMLNGEKWFV
                     TGGERAGFFIVLAWAGEKQASFLVDRDAPGLQIVRTPTFMHDPYASKHVELELTNCRV
                     PDSARLGGEGNEGTKRWFSLERLMIAARCCGAAERLIELARSWSLERSASGTKISQYQ
                     AIQFMLADSVTELNAARLMTYHAAQSWDRDDPSVLHGKTAMTKLYASEMAGRVADRAV
                     QIFGGRGYMTENPAERYYRELRVDRIWEGTSEIQRLIIARGMLKRGVGAYLMDV"
     gene            3005..4453
                     /locus_tag="VFT03_01400"
     CDS             3005..4453
                     /locus_tag="VFT03_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEU4846824.1"
                     /translation="MDLQVTRMNNFVGGGFVKAVRDEWERVIDPASGEVIAEVPRCAE
                     EDVDRAVEAASTAFEAWFETTPAERAQMLHKLADVLQEHAEELARLESRDVGKPITSA
                     RGELPFVVDNLRFFAGACRVMEGKAAGEYLRGYTSMIRREPVGVAGQIAPWNYPLMMA
                     VWKLGPALAAGNTVVLKPSEQTPLTTLKMAELAADIFPGGVLNVITGDGEPVGAGIVR
                     HPKVDMVSLTGDVATGKEVARAAAQTLKRVHLELGGKAPVIVFDDADIEAVVENLRYA
                     GYTNSGQDCTAASRVIAGPRAYDGLTSELASAVSTIKTADPAEGEHVEMGPVVSAVHQ
                     ERVLGFLERAKAGGDIEILTGGNETNGGRGFFVEPTVIAGPKQDSEIVQKEVFGPVVT
                     VQRFEDDEEALRWANDVSYGLAASVWTRDVGRALKAVRALRFGTVWINDHIAGVSEMP
                     HGGFKQSGYGKDMSAYALEDYTVIKHVMAKLD"
     gene            4607..5107
                     /locus_tag="VFT03_01405"
     CDS             4607..5107
                     /locus_tag="VFT03_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4846825.1"
                     /translation="MNNGSIPAGARIGHVHLKVSDLERAIRFYRDLMGFDLLMRFGDE
                     AAFLSAGGYHHHIGLNTWESAGGPPPPRGATGLYHFAINYPERRDLAVALKRLLDNGW
                     SIDGASDHGTHEAIYLHDPDHNGIELAWDRDPSEWPRRNGELSFTRRPLDFDGLMAEL
                     GERVAE"
BASE COUNT         1006 a         1632 c         1949 g          893 t
ORIGIN      
        1 cgcgcgccgg tccccggcgt ctcgcagatc ctcggcccgg ccgacgcaaa cccagctctc
       61 gtcgaagaag tgctcgagct cccaccggaa caccgagacg tccgcgtacg cccccgcggg
      121 caggctcatc tgcccgtcgg aactcagaga cgaagccagc agtttgcggt caaccggggt
      181 catgagcacg ctcctttaga tccgatccac tacgccccac gcgccatact ccgccgcgac
      241 agcggcccgc caacaccgct tgccgcaact taccaacttc agtataatcg tctttgaatg
      301 acggttcaag aatacgccag ggcggaaccg gctggtgggg ccctccggga cagagaggct
      361 tcgcgagggg tggaccgtgt cggtgggaag cttttccatc ttggcggagt gggagccgct
      421 cgctggtgtt ttcatctcca gccaggtcgt ggcggctgcg gtgccttcgt actgaacaag
      481 agttcaaaac acgatggcaa gatcacgggc ggcgcgaagg tgatggaggt ttgcgaagac
      541 ggcggtcgtc atcggggtga gaagggcagc gatacgtgag gaggagtggg tgatgccggt
      601 ctacgatgtc gcgatagtgg ggggcggcaa cctggggcta tggacggcgt accggctggc
      661 gcggcgtggt ttcgggcgcg tggcggtcct ggagcggggc tgggccggtg gtggggcgac
      721 gagccgttcg gctggggtgg ttcgccagca gggaggctcg gggacggcgg cgacgctggg
      781 ccagctcgcg agggagctat atctggagct gggcgaggag ctcgggttgg atccggggtt
      841 cgtagagact ggctactaca tcgtggcgga gacggaggag gagaaggaaa gcttccagag
      901 gctcgtggag gttcggcgcg aggcaggggt cgagaacgag tgggtcgagc ccgaggaggg
      961 caaggagcgc ttcccggacc tcgattggga ccgtttcgtg ggtgccactt acacctcgac
     1021 cgatgggtat gtgaacccgc agatagccgc ccgcaacgcc acgtgcgccg tcttatcctc
     1081 tgactccgtg gacctcttcg agatgtgcga ggtcagaagc atcgaggaga gaagcgggcg
     1141 ttatgcgctc gagaccgtct gcggcacgtt cgaggcggag cgggtggtgg acgctggcgg
     1201 gccgcggggc gcgcgagagg tcggggcgat ggtcggcgtg gaggtgcccg taatggcggt
     1261 caggcaccag atcgtcacgt tcccaaccat cgctgaggga gcggcgcacc cgttcccgct
     1321 gttcttcctg gtgggcaaag gctactacgc gcggcccgag gagcagggcg ctctgttggg
     1381 gatgagcaat gcctcggacg aggccgaccc ctcggagcgc taccagatcg acttcgactg
     1441 gggctacttc gagaagacgc gacccgattg ggagtcggcg tatccgccgc tcaaggggct
     1501 gccggtctcg cgggcgtggg cggcttccat agactacacg cctgaccacc tgccgatcat
     1561 cgacgagcct atggagggct tctacgtcct ggccgccggg gggcatggca tgatgtgggg
     1621 accggcgctg ggagagaaga tggcgggttt gatctccgag ggctcggtta gcggcgtgcc
     1681 gagggaggac gtggagctcg cgcggttctc gcgcgtgaag gatcaagaca gggtggagga
     1741 catgatctcc cttcccttcc ccaagaaatg aagggagatg aagctgtgac tgtggcagaa
     1801 cggccgacga tccccgagca cgtgcggttg ctgaaggccc gcatcgcccg gttcgtaagg
     1861 gaggagctcc acccgttcga ggcggagatc gcgcgccgcg gcgagatcga cgaagagaag
     1921 ctgggagcgc ttcgggaaaa ggcccgcgcg gcggggttca gcaacctcaa catgcccgag
     1981 gagtatggcg gaatggacct gcccgttgtg tcgcaggttg ccctggagga agaggcgggg
     2041 aagacgacca acggcctcgg ctacatggtc gctgatcggg gtcccagaga gctgctcgac
     2101 ctcgcgagcc ccgagcaggt cgagcgctac gtcatgccag tgatcagagg cgagacgcgc
     2161 gaggcgtggg cgatcaccga acccggagcc ggttcggaca taaaggatct cgcgaccacc
     2221 gccgaaagag acggcgaggg gtggatgttg aacggggaga agtggttcgt taccggcggg
     2281 gagcgcgcgg gattctttat cgtgctggcg tgggccggag agaagcaggc gtccttcctc
     2341 gtagacaggg acgcgcccgg gctgcagatc gtgcgcacgc cgacgttcat gcacgacccg
     2401 tacgcctcca agcacgtcga gctcgagctg acaaactgcc gggtccctga cagcgcgcgt
     2461 ctcggaggcg agggaaacga gggaaccaag cggtggtttt cgctggaacg gctcatgata
     2521 gctgcgcgct gttgcggggc cgccgagcgg ctcatagagc tggcgcgctc gtggtctctg
     2581 gagcggtcgg cgtccggcac gaagatctcg cagtaccagg ctatccagtt catgctcgcc
     2641 gactccgtga ccgagctgaa cgcggcgcgg ctcatgacct accacgcggc ccagtcatgg
     2701 gatcgagacg accccagcgt cttgcacggg aagacggcga tgacgaagct ctacgcttct
     2761 gagatggccg gccgcgtggc tgaccgggca gtccagatct tcgggggacg cggctacatg
     2821 accgagaacc ccgcagaaag gtactaccgg gagctccgcg tggaccgcat ctgggagggc
     2881 acgagcgaga tccagcgcct gataatcgcc cgcgggatgc tcaagcgggg cgttggcgcg
     2941 tacttgatgg acgtctgacg acccgccaag agcgtccgca gcagcaccta cgagcggaga
     3001 gggtatggac ctccaggtca cacgcatgaa caacttcgtc ggaggcgggt tcgtgaaggc
     3061 cgtgcgggac gagtgggagc gcgtgatcga ccccgcaagc ggcgaggtga tagccgaggt
     3121 gcccagatgt gcagaggagg acgtggacag ggccgtggag gcggcgagca cggcgttcga
     3181 agcgtggttc gagacgacac ccgcggagag ggcccagatg ctccacaagc tcgccgacgt
     3241 cctgcaggag cacgccgagg agctggcgcg gctcgagtcc agggacgtgg gcaagcctat
     3301 cacctcggcc cgcggtgagc tgccgttcgt agtggacaac ctgcgcttct tcgcgggggc
     3361 ctgccgcgtc atggagggca aggccgcggg cgagtacttg agagggtaca cgtccatgat
     3421 ccgccgcgag ccggtgggtg ttgcgggcca gatcgctccg tggaattatc ctttgatgat
     3481 ggcggtctgg aagcttggac cggcactcgc cgctggcaac accgtcgtac tcaagccctc
     3541 cgagcagacg cctctgacga ctttgaagat ggccgagctg gccgccgaca tcttccctgg
     3601 cggcgtcctc aacgttatta ccggcgacgg agagccggtc ggcgcgggga tcgtgcggca
     3661 ccccaaggtg gacatggtct ccctgaccgg cgacgtcgcc accggcaagg aggtcgcgag
     3721 ggcggcggcg cagacgctca agcgcgtcca cctggagctt ggcggcaagg cgcccgtgat
     3781 cgtcttcgac gacgcggaca tcgaggcggt ggttgagaac ctgcgctacg cgggctacac
     3841 caactcgggt caggactgca cggcggcctc ccgcgtcatc gccgggccca gagcctatga
     3901 cgggctcacc tccgagctgg cgagcgcggt ctctacgata aagactgccg acccggcgga
     3961 gggcgagcac gtcgagatgg gtccggtcgt ctccgctgtc caccaggaac gcgtcctggg
     4021 attcctggaa cgtgcgaagg cgggaggcga catagagata ctcacaggcg gcaatgagac
     4081 taacggcggc cgcggattct tcgtcgagcc gacggtcatc gccggcccga agcaggacag
     4141 cgagatcgtg cagaaggagg tcttcgggcc cgtcgtgacc gtccagcgct tcgaggacga
     4201 cgaggaggcc ctccgctggg ccaacgatgt ctcctacgga ctggctgctt cggtgtggac
     4261 cagggatgtg ggccgggccc tgaaggcggt gcgggcgctg cgattcggta cggtctggat
     4321 caacgaccac atcgccgggg tctccgagat gccccacggg ggcttcaagc agtccggata
     4381 cggcaaggac atgtccgcgt atgctctgga ggactacacc gtgatcaagc acgttatggc
     4441 aaagctcgat tagagcggcg ccaggtgcaa cggtggggga tgactgccag ggaggccgcg
     4501 acgctcgggc gtggcaaggt ggtgcttacc gtgacggact tgaacaggct cgaggttata
     4561 tcctttacgt cctgaagggt gcgggcgatc agagaggacg tcgagcatga acaatggatc
     4621 catccccgct ggggccagga tcggccacgt gcacctgaag gtctcggacc tggagcgcgc
     4681 catccggttc tatcgtgacc tgatgggctt tgacctcttg atgcgcttcg gcgacgaggc
     4741 ggccttcctc tctgcgggcg gctaccacca ccacatcggg ctgaacacgt gggagagcgc
     4801 gggcggtccg ccgcctccac gcggcgcgac cggactctac cacttcgcca taaactatcc
     4861 ggagcgccgt gacctcgccg ttgccttgaa gcgcctgctg gacaacggct ggtccatcga
     4921 cggcgcctcc gaccacggca cccacgaggc aatctacctc cacgaccccg accacaacgg
     4981 catcgagctc gcctgggatc gcgacccgag cgagtggccc aggaggaatg gcgaattgtc
     5041 cttcacgagg cgccccctcg acttcgacgg cctgatggcc gagctcggag agcgtgtcgc
     5101 ggaatagtcc cgttctcaat agcacgcctg cttcgcaggc ttgtcagcac ggcgcctggc
     5161 ttcgccgggc ggcctgtagt gacctcacag ccggcgtctt gccagcaggt acaacatgat
     5221 gaccatcacc acgactccga gcacgacaag ggccgcgaac aggacgaacg gcatcgcatc
     5281 agacacctct ctcctcgttg tcgcggacat ggcttggatg tttttgttac ccgattgtag
     5341 cgtgcttgta acatagtggc gagcgcgtcg gcgcaccgag gcctgacctc caatgggaag
     5401 gcaaaactct cgttcagacc gtctactctc tctgtacgct attacttcca gaggctggaa
     5461 gctccaaacc acatccgctc
//
LOCUS       DASTUV010000064        12397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1566047, whole genome shotgun sequence.
ACCESSION   DASTUV010000064 DASTUV010000000
VERSION     DASTUV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12397
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566047"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..769
                     /locus_tag="VFT03_01410"
     CDS             89..769
                     /locus_tag="VFT03_01410"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU4846826.1"
                     /translation="MRNLLHALALSAAVAALTTATVFVISYLSAPVAGVQVIGARMFS
                     ELEAWNAIPNRASLLTLNAATLQEEIESNPWVEGAVVTKNWDSGIVTVQVEERRAVLD
                     AEVDGRRMILAADGTRLPGLGGADLERVELDEDLLGEVVEFARVLDSNGVRLDSIDEA
                     GPGGIEATVEGHRVVFSGDISGGQARVLEGVMAEHPDAEIFDLRSPQRVVVGGSSSAG
                     ANGGQLAG"
     gene            779..1981
                     /gene="ftsA"
                     /locus_tag="VFT03_01415"
     CDS             779..1981
                     /gene="ftsA"
                     /locus_tag="VFT03_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU4846827.1"
                     /translation="MAQSLVYGIDVGTTKIVALAGQVDTRRGWVEVLSMGESPSHGLK
                     RGVVVDRQAASESVAAAIEACGVLGGPVNVGIAGSHISSFNTEVTVLNRSKNLTITDR
                     FVRRLEQEARQLDLHEDEQVVHVLPRGYVLDGSEGVNRPVGLAARKVTMRAHVVSGAV
                     SSIQNLLHVVEDCGVRVSRVVLEPLASAEACLLDSDRESGVVLIDVGGGTTDIAAFIK
                     GVLTHTAVIPIGGENFSADLAYGLKIPQHKSEKMKRRYGTVLSGAVDPVAAVKLDDRY
                     YNANFMSQILEYRAREVFEYVRESLEDAHLHNLLPGGAILTGGGSLLDGMTDLAEDIL
                     GMRARTATPRRVRGEIKPVQKPQYATSVGLLYFSAKHGDLGSKVKGARATSFGSIVEA
                     VRSWFRGG"
     gene            2006..3094
                     /gene="ftsZ"
                     /locus_tag="VFT03_01420"
     CDS             2006..3094
                     /gene="ftsZ"
                     /locus_tag="VFT03_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU4846828.1"
                     /translation="MEGTKAMLDAGTSYLAVIKVVGIGGGGTNAVNRMINSGLQGVEF
                     IAVNTDAQALQMTDADQKIHIGEKITRGLGAGADPKIGAEAAEENKAEIEEALRGADM
                     VFVTAGKGGGTGTGAAPVVAKIARDSGALTVGVVTRPFAFEGRRRATYAEEGIKRLKE
                     NVDALIIIPNDRLLQVAEKRTSMMDAFKMADDILRKGVQGITDLITVPGLINLDFADV
                     RTIMHDSGSALMGIGESSSENRGAEAAKLAISSPLLEASIEGATGIILNITGGSELGL
                     FEVNEAAEIVHNAAHQDANLIFGAVIDESYGEQVTVTVIATGFDQRLANQRRTERPVV
                     EPREEESSAPVAAQESDVLDIPAFLRRR"
     gene            3108..3872
                     /locus_tag="VFT03_01425"
     CDS             3108..3872
                     /locus_tag="VFT03_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEU4846829.1"
                     /translation="MSREPFVLERSGPGVTSAADGAAVYIMPDPEPEGARVWFFTRLG
                     GVSREPYVTLNVSKKVGDDPATVEENLSIIRRAMGGRPSAWVRQVAGDGVVEVSEAGL
                     AGEADALLTSERDLCLVVAVADCIPVALVGERGVAMVHSGWRGTLAGISGKAIRSMGE
                     QGVRAYVGPGIRGCCYEVSEELAEEFAAKYGAGVVSGRHLSLPEAIRTDLERSGVEEI
                     HDLGLCSGCRPDLFFSHRKQGPLTGRNLAAIAKVSP"
     gene            3869..4576
                     /locus_tag="VFT03_01430"
     CDS             3869..4576
                     /locus_tag="VFT03_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU4846830.1"
                     /translation="MRAQESIAIRENLARVRDEVSAALERSGRGPDDARILVASKYYE
                     PAHISALAEAGADLLGENRAEDLVRKQEAFGDRFEWHFIGHLQSRKAKIVVPRVSLIH
                     SVDSVRLIEELAKRSPEGGYVDVLFQVNVSGEETKYGVSEGEVEALLEAAAETGGRVR
                     ARGFMTLAPLVEDPETVRYVFAKLRVIRDRLRQSWSPHFDLSELSMGMSGDYAVAVEE
                     GATLVRIGRVLLEEDGP"
     gene            4586..5158
                     /gene="sepF"
                     /locus_tag="VFT03_01435"
     CDS             4586..5158
                     /gene="sepF"
                     /locus_tag="VFT03_01435"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU4846831.1"
                     /translation="MGVRDRLERLAAWFGFGVDDDDYYEGEEEGDRRYGKDPYANGTH
                     NDEPSPAVRRLGRAERSSAFGTSLGDLFGSESPSRGDRYSQQNQPHLRAVPDQRPTRV
                     SVLEPSSFNDAQSLADRFKRQQPVILNLQNVDGDLARRMVDFCAGLTYALDGQIQTVA
                     SRVFLLTPRNVEVSAEERKRIAERAFFNQL"
     gene            5162..5947
                     /gene="proC"
                     /locus_tag="VFT03_01440"
     CDS             5162..5947
                     /gene="proC"
                     /locus_tag="VFT03_01440"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU4846832.1"
                     /translation="MEKVGIIGAGKIGGSLLVRLAESEGFDLAASDIHPEQLEPYRGY
                     GVRTTSSNRELAERSDLVVAAVKPWDVAKVLSEVAPAFEGTDKALVSVAAGVSIASIS
                     EKLPSGTAVLRVMPNVAASVGLGSAVVTANEPGLSALPTVMDIFRHVGDVMELPERLF
                     DAATALHGSGPAYVALFADALIQAGVREGIPRDVARRLVVETIAGTAVLLKDRSAHQV
                     RDEVMTPGGTTAAAFVAMEKAGFVAAVYDGVNAATERARGLGG"
     gene            5950..6288
                     /locus_tag="VFT03_01445"
     CDS             5950..6288
                     /locus_tag="VFT03_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU4846833.1"
                     /translation="MALLLQTFAYSIANLLSAVVNYTYYILFGFIIASILFSWFPSYP
                     SSGFFQAIYDAVRAVTNPILWPIRSRIPMLRLGGFGIDLSPIIAIFGLSIARSLLLII
                     IDQFIRPVTG"
     gene            6295..7326
                     /locus_tag="VFT03_01450"
     CDS             6295..7326
                     /locus_tag="VFT03_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU4846834.1"
                     /translation="MPIRPIDVRRKEFRNGFRGYDANQVDDFLDAVADEFERTYMENS
                     RLREEVSGLRERLQQFEELEGSIRAALIHAEQAANDLRRTANWEADDLRKSAAREADL
                     TIRDAKARAHQMLAESSARVEAIQESYHALQEAKRKFAGDFRQLLKTYTEMMDTMEIS
                     SAREIEASLRQRLDTESIAVAREAAIHEQDDSGAFAPEPADAEPEVDEEIVAEAASAE
                     DDAATQLIEPEAASQPTPEEIDSEIAELEATEPEATEPEATEPEVEPSQEPVAQTGPE
                     DPGAQPDTEAEPATTDEPAAEEVDREEPTLAEESSSQRFFDRGESSSEEGERESRIFR
                     ASRFLRRRE"
     gene            7319..7654
                     /locus_tag="VFT03_01455"
     CDS             7319..7654
                     /locus_tag="VFT03_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU4846835.1"
                     /translation="MSKRFARSAREGTLLNLHVSPGAKSTSVEGLYGEGAIKLKVAAP
                     PVGGKANAEVERFLSKLLGVPRSDVNIIRGASSRDKTVLVRGATEAETHEALAAHLPW
                     IPCRSAAST"
     gene            7630..8523
                     /locus_tag="VFT03_01460"
     CDS             7630..8523
                     /locus_tag="VFT03_01460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4846836.1"
                     /translation="MSLRSFDVDPAEAGERLDRLAARRLDTSRNIIQRIIQEGLVRVD
                     GEVAGSSYRVRGGERVEARLPERDLEPEEIPVPVLFEDEFLLVVEKPAGLVVHPGAGN
                     PSGTLVNALLERGISGGEDPDRPGIVHRLDRDTSGLMVVAKGEPAYSKLVEMMAGRRV
                     RRGYRAVVVREGLPETGTVDSPVGRDPDNPTLMTAGVGRPAVTHFEVLQEAGGHAMLR
                     VQLETGRTHQIRVHLAAIGHPVYADPLYGTALPGRRLWLHAERLSFEHPVTGEHLSFE
                     SSIPEDLREAALALDASLAFW"
     gene            8585..9133
                     /gene="pyrR"
                     /locus_tag="VFT03_01465"
     CDS             8585..9133
                     /gene="pyrR"
                     /locus_tag="VFT03_01465"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU4846837.1"
                     /translation="MDRTSVHEHSKVLSADGIERSLRRISHEILERNASNLGGLALVG
                     VLTRGVPLARRISENIRLFEGLDVPVGSLDITLHRDDLAGEEPQVRGSHVPFEVAGRT
                     VVLVDDVLYTGRTARAAIDALLEIGRPAAIRLAILVDRGHRELPIRADHVGKNVPTSL
                     HERVLVNLMETDGEDGVIIVHD"
     gene            9126..10040
                     /locus_tag="VFT03_01470"
     CDS             9126..10040
                     /locus_tag="VFT03_01470"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU4846838.1"
                     /translation="MTRNFLSLEDATREDLRSILDLARAHARGEAGSPLAGKTVCLAF
                     FEASTRTAVSFELAARRCGADVISLSEKGSAIAKGESLIDTVVTLDSLGADAIVLRHP
                     SAGAAAFAARHTGAAVVNAGDGCGQHPTQALLDLYALSEAAGGFGELAGRRVAIVGDV
                     LHSRVARSVIPAFQAAGMKVALVAPRTLLPQDSGAWRLPILFSVDEALLWGASVLYML
                     RLQNERMTGARVPSVAEYARFYGVSRRHLEDGVLVMHPGPVNRGVEISGDVVLDGASL
                     IPDQVAAGVHVRSAILVLAAGAAHEVAA"
     gene            10037..11341
                     /locus_tag="VFT03_01475"
     CDS             10037..11341
                     /locus_tag="VFT03_01475"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU4846839.1"
                     /translation="MNGVRADLVIRHAHVLDPSNALDGVADVRIRGGRVAEIGQGLRG
                     TRELDADGLHLFPGFVDVHAHWRTPGREDEESVESGSASAAAGGFTGVVMMPNTDPVV
                     DRPVVVSGLVRRVEKESRVRTYVSAALHAGLKGERLTEMRLLKEAGAFCVSDDGLGTA
                     SAGVLRNGMLYARSAGLPVILHCEDHTLATGVVHDGVHSALAGVPGSPASSEVAATAT
                     ALVLAAETGAKVHITHVSTALSAALVGFFKDRADVTADTTPHHLTLSDGLVATLDGMY
                     RVNPPLRPEVDREGVNEALRDGILDFVATDHAPHASEEKELPFEEAAPGFLGHETAFA
                     ALYTELVLAGGGISLPRLIGAMSCSPGRWVGAGGGISTGAPADLALVDLSERWTVGRE
                     TLLSRSSNSPYLGRELTGRVVGTIVGGELVYDRTGVKFGARA"
     gene            11325..>12397
                     /gene="carA"
                     /locus_tag="VFT03_01480"
     CDS             11325..>12397
                     /gene="carA"
                     /locus_tag="VFT03_01480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU4846840.1"
                     /translation="MEHGRNRATVVLEDGSVFEGWSFASEGEVAGEVVFTTSMVGYQE
                     TVTDPSYRGQIVLFTYPLIGNYGVIPGDDESRRVQAAGVLVREYTPHPSNWASERSLA
                     ALLEESGVIGVEGLDTRALTRHLRDKGAMRGVISTTDHDTVSLREKATSHPSMVGLDL
                     ASTSSELTEPALLPALGEQRCRVAALDYGVKGSIYEELRSRGASVLAMPGSASAQEIL
                     ASNPDGVFLSNGPGDPAALEDAVSTLRPLLGEVPVFGICLGHQLLGLALGCETYKMPF
                     GHHGVNHPVRNLNTGRIEITSQNHGFAVREETLPSDVKLTHRNLYDGTIEGIESPSLR
                     AWSVQYHPESSPGPRDSGYLFDG"
BASE COUNT         2179 a         3898 c         4280 g         2040 t
ORIGIN      
        1 agcgcggcga cgtcgtgctc actctcggcg caggcgacat ctccagcgca ggcgaagagc
       61 tgcttgagct cctgggaaca tcccggcctt gaggaacctg ctccacgccc tcgcgctctc
      121 cgcggccgtc gccgcgctca ccaccgccac cgtcttcgtc atctcgtacc tgagtgctcc
      181 cgtcgccggc gtgcaggtga tcggagcccg catgttctcc gagttagagg cctggaacgc
      241 tatccccaac cgcgctagcc tcctcaccct caatgccgcc acgctccagg aagaaatcga
      301 gtctaatcca tgggttgagg gtgctgtagt gaccaaaaat tgggattccg gtatagttac
      361 ggttcaggtt gaggaacgtc gtgcggtttt ggacgccgag gtagacgggc ggaggatgat
      421 tttggcggcg gacgggacga ggcttccggg attaggtgga gcggacctcg agagggtcga
      481 gctggacgag gacctcttgg gagaggtcgt ggagttcgcg agggtactgg atagtaacgg
      541 cgtcaggctc gactcgatcg acgaggctgg tccagggggc atcgaagcca ctgtagaagg
      601 ccaccgcgtc gttttctccg gcgatatctc cggcgggcag gcgcgggttc tcgagggcgt
      661 catggccgag catcctgacg ccgagatctt cgacctccgt tcgccgcaga gggtggttgt
      721 cggcgggtcg tccagcgctg gggctaacgg cggacagttg gctgggtagg ggtggttcgt
      781 ggctcagtcg cttgtctacg gcatagacgt tgggaccacc aagatagtag ccctcgcggg
      841 gcaagtggac acgcggcgag ggtgggtcga ggtgctctcg atgggcgagt ccccgagcca
      901 cgggctcaag agaggtgtcg tggtggaccg acaggcagcg agcgagtcgg tcgcggcggc
      961 tatcgaggcc tgcggcgtgc tcggcggccc ggtgaacgtc gggatcgccg gtagccacat
     1021 ctcttctttc aacaccgagg tcacagtcct caaccgctcg aagaacctga ctatcaccga
     1081 ccgtttcgtg aggagactcg agcaggaggc gcggcaactg gacctgcacg aggacgagca
     1141 ggtagtccac gtcctccccc gcggctacgt cctcgacggt tccgaaggcg tcaaccggcc
     1201 ggtaggcctc gccgcccgca aggtcaccat gcgggctcac gtggtgagcg gggccgtctc
     1261 gagcatccag aacctgctgc acgtcgtcga ggactgtggg gtgagggtct cgcgcgtcgt
     1321 gctcgagccg ctggcctcgg ccgaggcgtg cctgctggac tctgaccgtg agtctggtgt
     1381 cgtcctcata gacgtcggcg gcggtacgac agacatcgca gcttttatca agggcgtgct
     1441 cacccacact gccgtcatcc ccatcggtgg agagaacttc tccgccgacc tcgcctacgg
     1501 gctcaagatc cctcaacaca agtccgagaa gatgaagcgg cgctacggca ccgtcctttc
     1561 aggcgccgtg gaccccgtcg ccgccgtcaa gctcgacgat cgctactaca acgcgaactt
     1621 tatgagccag atcctggagt accgcgcccg tgaagtcttc gaatacgtcc gcgaatcact
     1681 cgaggacgct cacctgcata accttctccc agggggcgcc atccttaccg gtggtggctc
     1741 cctgctcgat ggtatgacag acctggccga ggacatactc ggtatgcgtg cccggaccgc
     1801 taccccgcgc cgcgtccggg gcgagattaa acctgtacag aaacctcagt atgcaacctc
     1861 tgtaggtctg ttatacttct cggccaaaca cggtgacctt ggatcgaagg ttaaaggtgc
     1921 aagggcaacc agttttggta gcatagtgga ggcggtcagg agctggttca ggggagggta
     1981 aaccggcgag gccctagagc aggcgatgga gggtacgaaa gctatgttgg acgcggggac
     2041 tagctatctg gcggtaataa aagttgtcgg cattggcggt ggagggacca acgccgtgaa
     2101 ccgcatgatc aactccggct tacagggagt tgagttcata gcggtcaaca ctgacgctca
     2161 ggcgctgcag atgacggacg ccgaccagaa gattcacatc ggggagaaga tcacgcgcgg
     2221 ccttggcgcg ggcgcggacc cgaagatcgg ggccgaggcc gccgaggaga acaaggcgga
     2281 gatcgaggag gctctacgcg gggcggacat ggttttcgtc accgccggca agggcggcgg
     2341 cacgggcacg ggggctgctc ctgttgttgc gaagatagcc agggactcgg gagccttgac
     2401 ggtcggggtc gttaccaggc cgttcgcgtt cgaggggcga cggcgggcta cgtacgccga
     2461 ggaggggatc aagcgtctca aggagaacgt ggacgccctg atcatcatcc ccaacgacag
     2521 gctcttgcag gtggccgaga agcgcaccag catgatggac gccttcaaga tggccgacga
     2581 tattctgagg aaaggtgtcc agggcatcac cgacctgatc acggttcctg gcctgataaa
     2641 cctggacttc gccgatgtgc gtacgatcat gcacgactcc ggctccgcgc tcatgggcat
     2701 cggggagtcg agcagcgaga acaggggagc cgaggccgcg aagctggcga tctcgagccc
     2761 gctgctggag gcgagcatcg agggtgcgac ggggatcatc ctgaacatca cgggagggag
     2821 cgagctgggg ctcttcgagg tcaacgaggc cgctgagatc gtccacaacg ccgcccacca
     2881 ggatgcgaac ctcatcttcg gcgctgtgat agacgagtcc tacggcgagc aggtgacggt
     2941 caccgtgatc gccacgggct tcgaccagcg cctcgctaac cagcgccgca cggagagacc
     3001 ggtcgtcgag ccgcgtgagg aagagtcgtc cgcccccgtt gccgcccagg agagcgacgt
     3061 cctggacatc ccggcgttcc tccgccgccg gtaaccacta tcgggacgtg agccgggagc
     3121 ccttcgttct cgaacgctca ggtcccggcg tcacgagcgc cgcggacggc gccgccgtct
     3181 acataatgcc cgaccccgag ccggagggag ctagagtatg gttcttcacc cggctcggcg
     3241 gcgtctccag ggagccctac gttaccctta acgtctcgaa gaaggtcggc gacgacccag
     3301 cgaccgtcga ggagaacctc tcgatcatca gacgggcgat ggggggcaga ccttccgcgt
     3361 gggtgcgcca agtcgcgggg gacggtgtcg tggaggtgtc ggaggcaggg ttggcgggcg
     3421 aggctgacgc cctcctcacc tcagagagag atctctgcct cgtcgtggct gtcgcggact
     3481 gtatcccggt ggcgctcgtc ggggagcgcg gcgtcgcgat ggtccactca ggttggcgcg
     3541 gcacgctcgc ggggatctca ggcaaggcga tacgcagcat gggggagcag ggcgtgaggg
     3601 cctacgtggg gcctggcatc agaggatgct gctacgaggt ctcggaggag ctcgccgagg
     3661 agttcgcggc gaagtatggt gctggcgtgg tctccgggcg ccacctgtcg ttgcccgaag
     3721 cgatccggac ggacctcgag aggtcggggg tcgaggagat acacgacctc ggactgtgca
     3781 gcgggtgccg gcctgaccta ttcttctcgc accgcaagca aggaccgctt acgggcagga
     3841 acctggccgc gatagcgaag gtgtcgccgt gagggcgcag gaaagcatcg cgatccggga
     3901 gaacctcgcg cgcgtgcgcg acgaggtatc tgcggcgctg gagcggagcg gtcgtgggcc
     3961 ggacgacgcg cggatactcg tcgcaagcaa gtattacgag ccggcccata tctccgccct
     4021 cgcggaggcc ggcgccgatc tgctgggaga gaaccgggcg gaggacctcg tccgcaagca
     4081 ggaggccttc ggcgaccgct tcgagtggca cttcatcggc catctccaga gtcgcaaggc
     4141 gaagatcgtg gttccccgcg tgagcctcat acactctgtg gactcggtga ggctgatcga
     4201 ggagctcgca aagcgctcgc cggagggcgg ctacgtggat gtcttgttcc aggtcaacgt
     4261 aagcggcgag gagacgaagt acggcgtctc tgagggcgag gtcgaggctc tcctggaggc
     4321 tgcggctgag accggaggca gggtccgtgc cagggggttc atgacgctgg cgccgcttgt
     4381 tgaagacccc gagactgtac gctatgtttt cgcgaaactt cgagtgattc gtgataggct
     4441 acgccaaagc tggagcccgc acttcgacct ctccgaactc tcaatgggca tgagcggtga
     4501 ttatgctgta gcggtcgaag agggagctac tctggtcagg atcgggcgag tgctattaga
     4561 ggaggatggc ccgtaaggag gcaggatggg tgttagggat cgtctggagc gtctcgctgc
     4621 ctggttcggc ttcggcgtgg acgacgatga ctactacgaa ggcgaagagg aaggagaccg
     4681 tcgctacggc aaggaccctt acgcgaacgg aactcacaac gacgagccga gccccgcggt
     4741 caggcgcctc gggcgggcag agcgttcctc ggccttcggc acctccctcg gagacctgtt
     4801 cggcagcgag agcccgagca gaggcgaccg ctactcgcag cagaaccagc cgcacctgcg
     4861 cgccgtgccc gaccagcgcc ctacccgtgt gagcgtgctc gagccttcaa gcttcaacga
     4921 cgcccagtcg ctggccgacc gcttcaagcg ccagcaaccc gtgatcctga acctccagaa
     4981 cgtcgatggc gacctggcgc ggaggatggt ggacttctgt gccggcctga cctatgcgct
     5041 cgacggccag atccagaccg tcgccagccg ggtcttcctc ctgaccccgc gcaacgtcga
     5101 ggtgagcgcc gaggagcgca agcgcatcgc cgagcgggcg ttcttcaacc agctctgatt
     5161 cttggagaag gtcgggataa tcggagcggg caagatcggc ggctcgctcc tggtgcgcct
     5221 cgccgagtcg gagggcttcg acctcgccgc cagcgatatc caccccgagc agctcgagcc
     5281 gtacaggggc tacggggtgc gcaccacctc ctcgaaccgc gagctggcgg agcggagcga
     5341 tctcgtcgtc gccgccgtca agccctggga cgtggcgaaa gtcttgagcg aggtcgcgcc
     5401 tgccttcgag gggacggaca aggcgctcgt aagcgtcgcc gcgggcgtct cgatcgcgag
     5461 catctccgag aagttgccgt caggtaccgc ggtcctgcgc gtcatgccta acgtggccgc
     5521 ctcggtaggg ctcggctcgg cggtcgtgac ggccaacgag ccggggctgt ccgctttgcc
     5581 gacggtcatg gatatcttcc ggcacgtcgg ggacgtgatg gagctgcccg agcgcctctt
     5641 cgacgcggct acggctctgc acgggtctgg cccagcgtac gtggcgctct tcgccgacgc
     5701 cctgatacag gccggggtga gagagggcat cccgcgcgac gtcgcgcgcc ggctggtggt
     5761 ggagacgata gcagggacgg cggtactgct taaggaccgg agcgcccacc aggtgcgcga
     5821 cgaggttatg acaccaggcg ggacgacggc tgcggccttc gtcgcgatgg aaaaggccgg
     5881 gttcgtcgca gcggtctacg acggggtcaa cgcggcgacg gagcgggcgc gtgggctcgg
     5941 ggggtgaccg tggcactgtt gttacagacg ttcgcctaca gcatcgcgaa cctactttcg
     6001 gccgtggtga actacaccta ctacatcctc ttcggtttca tcatcgcctc tatcctcttc
     6061 tcctggttcc cgtcttaccc ttcgagcggt ttcttccagg cgatctacga cgcggtgcgg
     6121 gccgtgacca acccgatact gtggccgata aggagcagga tcccgatgtt gaggctcggg
     6181 ggtttcggga tcgacctctc cccgatcatt gccatcttcg ggctgtcaat agcccgcagc
     6241 ttgctgctga taatcatcga ccagttcatt cgacctgtaa cggggtgata aagcatgccg
     6301 attagaccga tagacgtgag gcgcaaggag ttccgtaacg gcttccgcgg ctacgacgcc
     6361 aaccaggtag acgacttcct ggatgccgtg gccgacgagt tcgagcgcac ctacatggag
     6421 aactccaggc tgcgcgagga ggtttctggc ctgcgcgagc gcctccagca gttcgaggag
     6481 ctggagggtt cgatccgggc cgccctcatc cacgccgagc aggctgctaa cgacctgcgc
     6541 cgcaccgcga actgggaggc cgatgacctc cgcaagagcg ctgcccggga ggcggacctt
     6601 acgatccgcg acgcgaaggc tcgcgcccac cagatgctag ccgagtcctc cgcgcgtgtc
     6661 gaagccatcc aggagtccta ccacgcattg caggaggcca agaggaaatt cgccggcgac
     6721 ttccgccaac tccttaagac ctacaccgaa atgatggaca ccatggagat ctcctccgcc
     6781 agggagatag aggcctccct ccgccagcgc ttggacaccg agtcgatcgc cgtcgcccgc
     6841 gaagccgcca tccacgaaca ggatgactcc ggcgctttcg cccccgaacc ggccgacgcc
     6901 gagcccgaag tagatgagga aatcgttgcc gaagcggcct ccgccgaaga cgacgcggcg
     6961 acgcagctca tagaaccgga ggccgccagc cagcctacgc cggaggagat agattcggag
     7021 atcgccgagc tagaggcgac ggagccggag gcaacggagc cggaggcaac ggagccggag
     7081 gtcgaaccgt cgcaggagcc cgtcgcccag acagggcccg aggaccccgg cgcgcaacca
     7141 gacacggaag ccgagcccgc gaccaccgac gagcccgccg cggaagaagt ggaccgggaa
     7201 gagccgacgc tggccgagga gagcagctcg caaaggttct tcgaccgcgg agagagctca
     7261 tccgaagagg gcgagaggga gagccgcatc ttccgcgcca gccgcttttt gcgccggcgt
     7321 gagtaagcgt ttcgcaaggt ccgctaggga aggaaccctc ctcaacctcc acgtctcgcc
     7381 cggcgcgaag agcacctcgg tcgaaggcct ctacggcgaa ggcgccatca aactcaaggt
     7441 cgcggcaccg cccgtaggcg gcaaggccaa cgctgaggtc gagcgtttcc tctcgaagct
     7501 tctcggcgtc ccgcgctcgg acgtcaacat catccgtggc gcgtccagca gggacaaaac
     7561 cgtcctcgtg cgtggcgcta cagaggccga gacccacgaa gcgctcgcgg cccacctacc
     7621 ctggatcccg tgtcgctccg cagcttcgac gtagatcccg ccgaggcagg cgaacgcctc
     7681 gaccgcctgg cagcccgccg cctcgatacc agccgaaaca taattcaacg cataatacaa
     7741 gaaggtctag tgcgggtcga tggcgaggtg gccggctctt cgtacagggt acggggtggg
     7801 gagcgtgtgg aggcgcggct gccggagagg gatctcgagc cggaggagat ccccgttcct
     7861 gtcctcttcg aggacgagtt cttgctggtt gtggaaaagc cggccgggtt ggtggtgcat
     7921 cctggcgcgg ggaacccgtc ggggacgctg gtcaacgcgt tgttggagag aggaatctcg
     7981 ggtggcgagg acccggatcg gcccgggatc gtccaccgcc tggacaggga cacctcgggg
     8041 ctgatggtgg tcgcgaaggg agagccagca tactcgaagc tggttgagat gatggcgggg
     8101 aggagggtgc ggcggggcta ccgggccgtc gtcgttagag agggactgcc cgagacgggc
     8161 acggtggact cgcccgtggg gcgggacccg gacaacccga cgctcatgac cgccggtgta
     8221 gggaggcccg cggtgaccca cttcgaggtg ttacaggagg cgggtggaca cgcgatgctg
     8281 cgggtgcaac tggagacggg caggacgcac cagatccggg tccatctcgc ggccatcggg
     8341 cacccggtct acgccgaccc gttgtacggg acggctctcc cggggcggcg tctctggctg
     8401 cacgctgagc ggctgtcgtt cgagcaccct gtaacggggg agcacctgag cttcgagtca
     8461 tccatccctg aggacctgcg ggaagccgct ctagctcttg acgcttctct ggcgttctgg
     8521 tagcgtctcg catgagagaa ccggccttta acggcgtcca gcgaggcgcg aagggggtta
     8581 cggattggac cgaaccagcg tccacgagca ctcgaaggtg ctctctgccg acggcatcga
     8641 acgttcgtta cgccgcatct cgcacgaaat cctcgaaaga aacgcatcca acctcggcgg
     8701 gctcgcgctc gtcggggtct tgacgcgtgg ggtccctctg gcacgccgca tttcggagaa
     8761 catccggctc ttcgaggggc tcgacgtgcc ggtgggttcg ctggacatta cgctgcaccg
     8821 cgacgacctg gctggtgagg agccgcaggt gagggggagc cacgtgccct tcgaagttgc
     8881 ggggcggacg gtcgtgctgg tggatgacgt cctgtacacc ggccggacgg ctcgggccgc
     8941 aatagacgcg ctgttagaga tcgggcgccc cgcggcgatc cggctcgcca ttctggtcga
     9001 tcgcggccac agggagctgc ccatcagggc ggatcacgtc gggaaaaacg tgcctacctc
     9061 gctccatgag cgggtgctgg tgaacttgat ggagaccgac ggggaggatg gggtgatcat
     9121 cgttcatgac taggaacttt ctgagtctgg aggacgccac tcgcgaggac ttgaggtcga
     9181 tactggacct ggcacgcgcc cacgcgagag gggaggcggg gagcccgctc gccggaaaga
     9241 cggtctgcct ggccttcttc gaggcatcga cccggacggc ggtatctttc gagctcgcag
     9301 ccaggcggtg cggagcggac gttatctccc tctccgagaa gggctcggcc atagcgaaag
     9361 gcgaatctct catagacaca gtcgttaccc tcgacagcct cggagcagac gctatagtcc
     9421 tccgccaccc gtccgccggc gcggcagcct tcgccgcccg ccatacgggt gccgcggtgg
     9481 tcaacgccgg ggacgggtgc ggccagcacc cgacgcaggc cctcctcgac ctctatgcgc
     9541 taagcgaagc ggcgggcggg ttcggcgagc tcgcgggccg gcgggtcgcc atcgtgggcg
     9601 atgtactgca cagccgggtc gcccgcagcg tcatccctgc gttccaggcc gccgggatga
     9661 aggtcgccct cgtcgcccct cgcacgctcc tcccgcagga cagcggagcc tggaggttgc
     9721 cgatcttatt ctcggtggac gaggcgctcc tctggggggc gagcgtcttg tacatgctgc
     9781 ggctgcagaa cgagcgcatg accggcgcgc gggtcccttc tgtcgccgag tacgcgcgct
     9841 tctacggagt ctccaggcgg cacctggagg atggggtact tgttatgcat cccggcccgg
     9901 tcaacagggg ggtcgagatc tccggcgacg tcgtcctcga cggagcgtcc ctgatccccg
     9961 accaggtcgc cgcgggggtc cacgtccgtt cggcgatact cgtgctcgcc gccggcgccg
    10021 cccacgaggt cgctgcttga acggggtcag ggctgacctc gtcatccgcc acgcccacgt
    10081 cctcgacccg agtaacgccc tcgacggcgt cgccgacgtg cgtatccgtg ggggccgcgt
    10141 cgccgagata ggccagggct tgagggggac gcgcgagctg gacgccgacg ggctccatct
    10201 cttccccggc ttcgtggacg tgcacgctca ctggcgcacg cctggacgcg aggacgagga
    10261 aagcgtcgag agcggctccg cgtcagccgc cgccggcggc ttcaccgggg tcgtgatgat
    10321 gcccaacacg gaccccgtcg tggacaggcc ggtcgtcgtg agcggcctcg tccggcgcgt
    10381 cgagaaagag tccagggtgc ggacctacgt ctccgccgcc ctgcacgcgg ggttgaaagg
    10441 cgagcggctc acggagatgc gcctgctcaa ggaggccggc gcgttttgcg tctcggacga
    10501 cgggctcgga acggcttcgg ctggcgtctt gcgcaacggg atgctctacg cccgctccgc
    10561 cggcctgccc gtgatcctgc attgcgagga ccacacgctc gccacgggcg tcgtgcacga
    10621 cggagtccat tccgcgctcg ccggagtccc gggatcgccc gcgagctccg aggtcgcggc
    10681 gacggcgacg gccctcgtgc tagcggcgga gacgggggca aaggtccaca taacccacgt
    10741 ctcgaccgcg ctctccgcgg ctctcgtcgg gttcttcaag gaccgggctg acgtcaccgc
    10801 ggataccacg ccgcaccacc tgacgctgag cgacgggctc gtcgccacgc tcgacgggat
    10861 gtacagggta aacccgcccc tcaggccgga agtagaccgc gagggggtta acgaagcctt
    10921 gcgcgatggc atcctcgact tcgtggcgac ggatcacgcg ccgcacgcct cggaggagaa
    10981 ggagctgccc ttcgaggagg ccgcgccggg tttcctgggg cacgagaccg ccttcgccgc
    11041 cctctacacc gagctggtcc tcgcgggggg tgggatctcg ctcccacggt tgatcggggc
    11101 gatgagctgc tctccggggc gctgggtcgg cgcgggtggt gggatctcga caggggcgcc
    11161 cgcggacctc gcgctcgtcg acctctcgga gagatggacg gttggccgtg agacgctcct
    11221 gagccggtcc tcgaactcgc cttatctcgg gcgtgagctg acgggtcggg tagtgggtac
    11281 tatagttggc ggagagctgg tctacgacag gacgggagtg aagtttggag cacgggcgta
    11341 accgcgcgac ggtggtactc gaagacggct ccgtcttcga ggggtggagc ttcgcgagcg
    11401 agggagaggt cgcgggcgag gtcgtcttta ccaccagcat ggtcggctac caggagaccg
    11461 tcacagaccc ctcttaccgc ggccagatcg tgctcttcac ctaccccctg atagggaact
    11521 acggtgtgat ccccggcgac gacgagtccc gaagggtcca ggctgcgggc gtcctcgtgc
    11581 gcgagtacac cccgcatccc agcaactggg cgagcgagcg ctccctcgca gccctcctcg
    11641 aagagtccgg cgtgatcggg gtcgaaggcc tggacacccg cgcccttacc cgtcacttga
    11701 gggacaaagg tgcgatgcgg ggtgtcatct ctaccacaga tcatgacacc gtctccttgc
    11761 gcgagaaggc tacatctcac ccctcgatgg tcggcctgga cctcgcctcc acgagctccg
    11821 agctgaccga gcctgcgctt ctgcccgccc tcggcgagca gcgctgccgg gtcgcggccc
    11881 tcgactacgg cgtgaagggc tcgatctatg aggagctcag gagccggggc gcctccgtgc
    11941 tagctatgcc tggctcggcg agcgctcagg agatactcgc gtcgaacccc gacggcgtct
    12001 tcctctccaa cgggccgggt gacccggctg ctctcgaaga tgcggtgagc acgctgcgcc
    12061 cgctacttgg cgaggtgcct gtcttcggca tctgcctcgg gcaccagttg ctcggcctcg
    12121 ctctcgggtg cgagacgtac aagatgccct tcgggcacca cggcgtcaac caccccgtac
    12181 ggaacctgaa cacggggagg atcgagatca ccagccagaa ccacggcttc gctgtgcgcg
    12241 aggagacgct gccatcagac gtgaagctga cccaccgcaa cctctacgac ggcaccatag
    12301 aaggcatcga gagcccctcc ttgagagcct ggagcgtgca gtaccacccg gagtccagcc
    12361 ctggcccccg ggactcgggc tacctcttcg acgggtt
//
LOCUS       DASTUV010000065         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_135300, whole genome shotgun sequence.
ACCESSION   DASTUV010000065 DASTUV010000000
VERSION     DASTUV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135300"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFT03_01485"
     CDS             <1..833
                     /locus_tag="VFT03_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4846841.1"
                     /translation="VIITAGIDLSVGSVLVLSSVIAAQTMVALSGTDQQVQNYTYPNQ
                     AVGIPVGIAAGLLTGLVCGLINGLLVTKLKLTPFIATLGTLGVFLGTAQILSGGTNVP
                     YVPPAIQIQIGTRQLLGILPVPIAIGLIVVVVAILALHRTQFGRYTYAIGSNAEAARR
                     VGIDVDRHLLKVYTLSGFLAGLAGIIDVARFNTASVGAHTLDNLAAISAAVIGGTSLF
                     GGIGTIVGSVVGTFIPAVLRNGFVIHGVQPFWQEVAIGIVLILAVFIDQRRRSHEERM
                     "
     gene            853..1863
                     /locus_tag="VFT03_01490"
     CDS             853..1863
                     /locus_tag="VFT03_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4846842.1"
                     /translation="MSNHIRVGRRISRREFLGVAAAGAGVAFLGGCGGGGGGQGGAES
                     YRLTLIVGVTGDEFYTTMECGAQAAARELGADLTVQGPEEFSAAEQTPILNAAIQANP
                     DAILIAPTDRAAMVAPIQSAVNKGITVVLVDTTIEKEDIALAQISSDNVEGGRQAGEA
                     LAKQIGEEGKVLVISVKPGISTTDQRQQGFEQAIEEYPNIEYLGTEYCNDDPTQAASI
                     TTSTLQSNPDLKGIFGANVFSGQGAGTGVRQTGKKDQVSVVAFDASPTQVEDLRRGNV
                     DVLIAQHPNDIGDKGVRIAVDYLKSDKEPSSKHITTGFTTVTRENIDEPEVKEYLYKA
                     QC"
BASE COUNT          412 a          618 c          665 g          316 t
ORIGIN      
        1 tcgtgatcat caccgccggc atcgacctgt cggtcgggtc ggtgctggtg ctctccagcg
       61 tgatcgccgc ccagaccatg gtggcgctgt ccggaacgga ccagcaggtc cagaactaca
      121 cttacccgaa ccaggccgtg ggcatacccg tagggatagc ggccggcctc ctgacagggc
      181 tggtatgcgg gctcatcaac gggctgctcg tcacgaagct caagctgacg ccctttatcg
      241 ccacgctcgg caccctcggg gtattcctgg ggacggcgca gatcctctcc gggggcacga
      301 acgtccccta tgtcccgcca gcgatacaga ttcagatcgg tacgcgccag ctcctgggaa
      361 tcttgccggt gccgatagcc atagggctga tagtggtggt agtggcgatc ctcgccttgc
      421 atcggaccca gttcggacgc tacacctacg ccataggctc gaacgcggag gccgcacgca
      481 gggtgggcat cgacgtggac cggcatctct tgaaggtcta caccctgagc ggtttcctcg
      541 cgggactggc ggggatcata gacgtggccc ggttcaatac cgcctcggtg ggcgcgcaca
      601 ccctggacaa cctggcagcc atctcggccg ccgtcatcgg gggcacgagc ctcttcgggg
      661 gcatcgggac catcgtgggg tcggtcgttg ggacctttat acccgcggtc ttgaggaacg
      721 ggttcgtgat acacggcgtc cagccgttct ggcaggaggt agccatcggg atagtcctga
      781 tcctggccgt ctttatcgac cagcggcgcc ggagccacga agagcggatg tagtagggaa
      841 aggaggatag agttgagcaa ccatatccgg gtaggacgca ggatctcgcg ccgcgagttt
      901 ctcggcgtcg ctgccgcggg cgccggggta gcgttcctcg ggggctgcgg aggcggaggt
      961 ggcggccagg gcggcgctga gagctacagg ctcaccctga tcgtgggcgt caccggcgac
     1021 gagttctaca ccaccatgga gtgcggagcg caggctgcgg ctcgggagtt gggtgccgac
     1081 ctgaccgtgc aggggccaga ggagttctcg gccgccgagc aaaccccgat cctgaacgcg
     1141 gccatccagg ccaaccccga cgccatccta atagcgccga ccgacagggc ggccatggtc
     1201 gccccgatcc agagcgccgt caacaagggc ataaccgtag tgctggttga cacgactatc
     1261 gagaaggaag atatcgcact ggcccagatc tcctccgaca acgtcgaggg aggcaggcag
     1321 gcgggcgagg ccctcgcgaa gcagatcggt gaagagggca aggtgctcgt cataagcgtg
     1381 aagcccggca tctccaccac ggaccagagg caacagggct tcgagcaggc gatagaagaa
     1441 tacccgaaca tcgagtatct cggaaccgag tattgcaacg atgatcccac ccaggcggcg
     1501 tccatcacca cctcgaccct ccagtcgaac ccggatctga aagggatctt cggcgccaac
     1561 gtcttctcgg ggcagggcgc cggaaccggg gtccggcaga cgggcaagaa ggaccaggtg
     1621 agcgttgtag ccttcgatgc ctcgccgaca caggtggagg acctgcgaag aggcaacgtg
     1681 gacgtgctca tcgcccagca cccgaacgac ataggcgaca agggcgtccg gatagccgtg
     1741 gactacctca agtctgataa ggagccgtcc agcaagcaca ttacaaccgg cttcacgacg
     1801 gtaacgcgtg aaaatatcga cgagcccgag gtcaaggagt acctctacaa ggcacagtgc
     1861 tagggtccac aagaaaccgg ccagtgagca cgtcacgccg aaagctcgcc ggctgctaaa
     1921 ctgcgggcca tggccgcgaa ggtgcgcata aggcccgagg tcataaccga gcaccgcctg
     1981 cgcatcgaga tgtacgggct cgaggacgag g
//
LOCUS       DASTUV010000066         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_249650, whole genome shotgun sequence.
ACCESSION   DASTUV010000066 DASTUV010000000
VERSION     DASTUV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249650"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..1543)
                     /locus_tag="VFT03_01495"
     CDS             complement(365..1543)
                     /locus_tag="VFT03_01495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4846843.1"
                     /translation="MEVRGYRAGDEASLARLGAGAFGENDGYWKEYYKPGGNGRVDLE
                     LVRLIEEDGDIRASAAVLPLEVFVDGRPVPMGGIAAVATDPAYRRRGFAGELMRDVLR
                     TMREREMHLSLLHPFAQVFYRAYGWELATETIAYTLKPTDLPTSSEQRRVRAYREGDL
                     PEMMDLLEGEAAGRSCCVRRSEARWRSLVSRKDWQAAVYEREGRLEGYIYYRMSDWRD
                     RKPRRTLSVWELVWNTVGAWRALISFLAALDPQVFEIKHSTPCGEPLHPYLRNSFVKA
                     EIVPEFMLRLVDVEGALNDLSRTAEAPLVLVVSDDVIPENAGSYTVGNGEVVRGEEEA
                     EARVSLDVRQLAQLYAGYLPAGQLARYGLVKPGSPEALELLEALFPVDDPWVYSPDHF
                     "
     gene            complement(1613..1843)
                     /locus_tag="VFT03_01500"
     CDS             complement(1613..1843)
                     /locus_tag="VFT03_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846844.1"
                     /translation="MARRELKRRDPDRTVDLCRPCHGNVHAALSNGDLGRMYDSLEAL
                     SAHPDVRKFTDWVRDKPHSRVRSRRPSTRDEA"
     gene            complement(2110..>2537)
                     /locus_tag="VFT03_01505"
     CDS             complement(2110..>2537)
                     /locus_tag="VFT03_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846845.1"
                     /translation="APAFAQATAVGGSVQYADCDQIQAAATAQYNTGNQQNAAGIAQE
                     LGVSQNQVLACLGSIAAGRDVNINAASASASATSTATAKMKATSTATAGGGGGGGGGG
                     SAAAAAGGALPSTGGASLLALGAGALLVAGGLLARRIVR"
BASE COUNT          442 a          866 c          797 g          432 t
ORIGIN      
        1 ccgtcgtaga gcatctcgta gcggtaaccg ccagttagca cgctgccttc ggcagcttgt
       61 cagcacggcg cttcgcgcct tgtcagcacg cttcggccct tcgggcctcg ctctcagcac
      121 gccctcttcg agggcttgtc agctagaaag ccaggaggac gcgacatggt gcttccctgc
      181 gctagtctaa gcttataccc aaaaatgtct ccaagcgtga ccgggtgtga acccaacggc
      241 agagagaata gctgaaatgc tgaaaagcga gggcctttag taaaggctcg agcgtgctga
      301 caaggcgtct ggcgaagcca ggcgccgtgc tgacaagctg cctgaaaggc agcgtgctga
      361 accgctagaa atgatccggc gaatagaccc acgggtcgtc caccgggaat aacgcctcca
      421 gaagctccag cgcctccggc gagcccggct ttacgagacc gtagcgggcg agctggccag
      481 ccggaagata cccggcgtag agctgcgcga gctgacgcac gtcgagcgag acgcgtgcct
      541 ccgcctcctc ctccccgcgc acgacctccc cgtttccgac tgtgtaggag ccggcgttct
      601 ccgggatgac gtcgtcagag accaccagta ccagcggggc ttccgcggtg cggctcaggt
      661 cgttcaatgc gccctcgacg tctaccagac gcagcatgaa ctccggcact atctcggcct
      721 tgacaaagga gttgcgcagg taagggtgga ggggttcgcc gcagggggtg ctgtgtttga
      781 tctcgaagac ctgcgggtcc agggccgcga ggaaggagat cagggcccgc cacgcaccca
      841 ccgtgttcca cacgagctcc cagaccgaga gcgtgcgccg tggcttgcgg tctctccagt
      901 cggacatccg gtagtagatg tatccttcca gccgtccttc ccgctcgtag acggctgcct
      961 gccagtcctt gcgggagacc aggcttcgcc accgcgcctc gctccggcgc acgcagcagg
     1021 aacgcccggc cgcctcaccc tcgaggaggt ccatcatctc gggcaggtcg ccctccctgt
     1081 aagcccggac gcgtctctgc tcgctgctcg tagggaggtc ggtaggtttg agggtgtagg
     1141 cgatcgtctc ggtggcgagc tcccacccgt acgcccggta gaagacctgt gcgaagggat
     1201 ggagcaaaga gaggtgcatc tctcgctcgc gcatcgtccg caggacgtcg cgcatgagct
     1261 caccggcgaa accccgtcgg cggtacgccg ggtccgtcgc gacggccgcg attcccccca
     1321 tagggacagg ccggccgtcc acgaagacct caagcggcaa taccgccgcg ctcgccctga
     1381 tgtccccgtc ctcttcgatg agacgcacga gttccaggtc gacacggccg ttcccccctg
     1441 gcttgtagta ctccttccag tagccgtcgt tctccccgaa cgcgccagca ccaaggcgcg
     1501 ctagcgatgc ctcgtccccc gcccgatacc cgcgtacctc caagacattc ctcccagacc
     1561 ggtgaccagg cctgattcta gcccgacgag cccgaccgga cacgggtggt agtcaggctt
     1621 cgtcccgcgt agaaggccgg cgagatctga cccgactatg aggcttgtcg cgcacccagt
     1681 cggtgaactt tctgacgtcc gggtgggcgg agagggcctc cagggagtcg tacatccttc
     1741 cgaggtcgcc gttggagagc gcggcatgca cgttgccgtg gcaaggtctg caaaggtcca
     1801 cggtccggtc agggtcacgg cgcttgagct cgcgtcgtgc catcttcctc cgtctcatcc
     1861 tccggacctg ggagcgtggc agtagatggt ggcgggtgag gcggtcccgc ccgacgaacc
     1921 tgccgcacag cccgcacgtc ccatccgcgc cgggttcggg ttttcgatct tccgtaatca
     1981 ccccgagatt ataccgtccg ggcgatgctc ctttggcctt ggcaagcgag atatggccgt
     2041 cggccgcggg cacgaagaag cgggcccccg aaggagcccg cttcttctaa tgcgctgctc
     2101 ggttagttac taccgaacga tcctgcgggc cagaagacca ccggccacca gcagggcacc
     2161 agcgccaagc gccagcagcg atgcaccacc cgtgctcggg agcgcgccac cagcagccgc
     2221 cgctgctgag ccgccaccgc caccgccgcc gccgccacca gccgtagcgg tcgaagtcgc
     2281 cttcatctta gcagtcgcag tcgacgtagc cgaagccgaa gccgaagcgg cgttgatgtt
     2341 cacgtcacgg ccagcagcga tgctgccgag gcacgcgagc acctggttct ggctgacgcc
     2401 aagctcctgc gcgatgccgg ctgcgttctg ctggttacca gtgttgtact gagccgtagc
     2461 cgcagcctgg atctggtcac agtccgcgta ctggacgctg ccaccgacgg ccgtcgcctg
     2521 ggcgaaagcc ggggcgg
//
LOCUS       DASTUV010000067         5607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_993343, whole genome shotgun sequence.
ACCESSION   DASTUV010000067 DASTUV010000000
VERSION     DASTUV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993343"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VFT03_01510"
     CDS             <1..1530
                     /locus_tag="VFT03_01510"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU4846846.1"
                     /translation="PHYITSEMRSLFLENYEYSSFDLREEDRVQAEVFSSRMAGQILS
                     GDTPAELIEHYTEYSGRMRPLPEWILGGAVVGVQGGTDKVLDVYDELEALGTPVAALW
                     LQDWVGQRETSFGTQLWWNWELDEDHYRGWDQLREKLERDDVRLMTYIGPWLADDVTE
                     KENHRRNLFEEAARKGYLVKNREGEPYRVKTTDFSAAFFDLTNPEARAWVKDLIKEEL
                     IGNGVSGWMADFGEGLPYDVVLYSGADPKSYHNRYAEEWAETNREAIREAGRGEDIVF
                     FNRSGYTRSPRYSTLFWLGDQLVDWNKHDGIKSAVTGLLSSGFSGYALEHSDIGGYTA
                     IDNFLLKYHRSKELLMRWIELGAFTAVFRTHEGNRPGVNHQIYSDEETLRHFDRFARV
                     YAAWKPYRIELVREAAATGLPVVRHPFVHYPDDPEVLGLQYQFLVGPDLMVAPVLDPG
                     EDMVEVYLPAGRWVHLWSGRKYGSPDKGVYETVSAPIGEPAVFYEESSDVGRLFSKIV
                     Q"
     gene            complement(1594..4461)
                     /locus_tag="VFT03_01515"
     CDS             complement(1594..4461)
                     /locus_tag="VFT03_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4846847.1"
                     /translation="MMGERNEPLAERLRDALDGRLEGYLRTDEASRALWSTDASIYLR
                     RPVGVVVARSEEDVKLALTAARELGLPITPRGTGTSLAGQATAPGLALDLSEMQKVFA
                     LDLEEKRCSVEPGTIQGELNALVEPHGLVFGADTSTSDVATLGGMVGNNSAGMRSIVY
                     GTTADQVLSLRCVLASGETVELRSLSREEAKRRASHADAASRILRGALEIEERYEDEI
                     KRRFPRMIRRVSGYGLDALVDPETVDLTRLVCGSEGTLAVVTRADFRLAELPPRRALA
                     SLEFDSLAAAARATVELLKLGPSALEMLDDVAIGRARASTAYAGSTRFVQGDPKALLL
                     VEWSGTREELDERFAGLEEMAREVGALHSTPLRTKGEMQATVKLRKSILPLLLGTTER
                     EKPVAFVEDAAVPPDRLEEFIGRFEEIVEKNGTWACFYGHASVGCLHVRPALDTSDPD
                     GVARMRRIAEGVADLVVDCGGSLSGEHGDGLARSEFLERMYGPEILRAFSEVKALFDP
                     EGMLNPGVIVAPPPMDRQLRIGPGRRRISINTNLDFSHQGNFAAAVELCNGSGFCRKK
                     TGGTMCPSYMVTLDEKDTTRARANLLRSVIDGTLPPEELTGERMEEVMDLCVGCKACK
                     TECPSQVDVASMKTEVLSQMGKTHGFSVRQKAAGHIRRQLALAALSPSLYNALAGTRP
                     ARRAAALAGIDPRRSLPKVAHKTFSKRFPSLPQGEGPAEVALFNDTWNEYQRPEVGVG
                     AVRLLAAAGAKVYLPEVVCCGRPMLSEGLVDEARKKARRNVDLLYPLVERGVPLVGLE
                     PSCILTIRDDYAKLLPKDERVRKLAGATRLFEEALLELDPELPLHQGSPVLLHGHCHQ
                     KALVGTGPTERALSLASGADVRVVDSGCCGMAGLFGYEAGHYEVSMKMGERRLFPAVR
                     GSEGRVVVAPGTSCREQILDGTGRRALHPAEYLAALLDGAA"
     gene            4512..5354
                     /locus_tag="VFT03_01520"
     CDS             4512..5354
                     /locus_tag="VFT03_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4846848.1"
                     /translation="MPRRRLRLDRLRIMSFNVRGSFRDSGTPNAWPERAELNVETIRH
                     HTPDVIGFQELQSGNLETYGETLPRYDHLPGPEYGNAARPSLNAIFFDAGRLRLLGAG
                     GFWLSMTPERYSSSWDSRAVRSANWAYFEPAFAGPSFLHLNTHLDHVSERARVEGSRL
                     IIRKVARLLQRYGEDTPVLVTGDFNCRPRNPAYRSFVENGFVDTYLAASNEDAGGAHT
                     FHAFKGSRYRDAHPHLGPRRIDWILLKDPLHRLRVVSHYIVRDHAGERYPSDHYPVLA
                     DLAL"
     gene            complement(5423..>5607)
                     /locus_tag="VFT03_01525"
     CDS             complement(5423..>5607)
                     /locus_tag="VFT03_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU4846849.1"
                     /translation="PERHRYVLLRRYGLDDRDVATLAQLSDELDISRERVRQLQREAE
                     RMLANGEFGAAFSNAA"
BASE COUNT         1008 a         1829 c         1844 g          926 t
ORIGIN      
        1 ccgcactaca tcacgagcga gatgcgttcc ctgtttctgg agaattacga gtactcctcg
       61 ttcgacttgc gggaggaaga cagggtccag gccgaggtgt tctcatcccg gatggccgga
      121 cagatcctga gtggagacac cccggccgaa cttatagagc actacacgga gtactctggc
      181 aggatgcgcc ccttgccaga gtggatcctc ggcggcgccg tcgtcggcgt gcagggcggc
      241 acggataaag tcctcgacgt atacgacgag ctagaagccc tcggcacacc cgtcgccgca
      301 ctctggctgc aagactgggt cggccaacga gagaccagct tcggcacgca gctgtggtgg
      361 aactgggagc tcgacgaaga tcactatcgc ggctgggatc aactccggga gaagctggag
      421 agggacgacg tgaggctcat gacctacatc gggccgtggc tcgcagacga tgtgacggag
      481 aaggagaacc accgccgcaa cctcttcgag gaggcggcga gaaagggtta cctggtaaag
      541 aatcgggagg gcgaacctta cagggtcaag accaccgact tctcggcggc attctttgac
      601 ctgacgaacc cggaggcgcg agcctgggtc aaggacctta tcaaggagga gctcatcggc
      661 aatggcgtct caggttggat ggcagacttc ggggaggggc ttccgtacga tgtagtgctc
      721 tactccggtg cggacccgaa gagttaccac aatcgctacg ccgaggagtg ggccgagacc
      781 aaccgcgagg cgatccggga ggcgggacga ggagaggaca tcgtcttctt caaccgctcc
      841 ggttatacac ggagcccccg ttacagcacg ctgttctggc ttggtgatca actcgtcgac
      901 tggaacaagc atgacggcat aaagagtgcc gtcaccggcc tgctctcgag cgggttctcg
      961 ggctacgccc tggagcactc ggacatcggc ggctacacgg ccatagataa cttcctgctg
     1021 aagtatcatc gctccaaaga gttgctcatg cgctggatcg agctcggagc cttcacggcc
     1081 gtcttccgca cccacgaggg caacaggccg ggggtgaacc accagatcta ctccgacgag
     1141 gagactttaa ggcacttcga ccgcttcgcc agggtctacg ccgcctggaa gccttacagg
     1201 atagaactcg tgcgcgaagc tgccgcgacc ggtctccccg tagtccggca tccgttcgtc
     1261 cactaccccg acgatccaga ggtgctcggc ctgcagtatc agttcctggt cggccctgac
     1321 ctgatggtcg caccggtact cgatcccggc gaagacatgg tcgaggtcta tcttccagcg
     1381 ggcaggtggg tgcacctctg gtcgggccgg aagtacggct ctccggacaa gggagtttac
     1441 gaaacggtga gcgctcctat cggcgaaccg gccgtgttct acgaggaaag ctctgatgta
     1501 ggacgcttgt tcagcaaaat cgtacagtaa ttctagaagt gccctcgcta tccaatgagt
     1561 ctcgactcaa ggcgacgcgc ccgccaacgc actctaggct gcaccgtcga gcagcgccgc
     1621 caggtactcg gcgggatgca gcgcgcggcg gccggtgccg tccaggatct gctcccggca
     1681 agaggttccg ggcgccacca cgacacgccc ttcggacccc cgcaccgccg ggaagagccg
     1741 cctctccccc atcttcatcg agacctcgta gtggcccgcc tcgtagccga agagcccggc
     1801 catcccgcag caacccgagt cgaccaccct gacgtcggcg ccggacgcca gggagagcgc
     1861 tcgctccgtc gggcccgtac ccacgagcgc tttctggtgg cagtggccgt gtagcaatac
     1921 aggcgacccc tgatgcaaag gcagctccgg gtcgagctcc aacaatgcct cctcgaagag
     1981 gcgggtggcc ccggcgagct tccggacacg ttcgtctttg gggagtagct tcgcgtagtc
     2041 atcgcggatg gtgaggatgc agctcggctc gaggccgacg agcgggacgc cgcgctcgac
     2101 gagaggatag aggaggtcca cgttgcgccg cgctttcttg cgcgcctcgt ccacgagccc
     2161 ctcggagagc atggggcggc cgcagcagac gacttctggc agatagacct tcgcccccgc
     2221 ggcggcgagg aggcggactg ccccgacgcc gacctctggg cgctggtact cgttccaggt
     2281 atcgttgaag agcgcgacct ctgccggtcc ttctccctga ggaagggacg ggaaacgctt
     2341 cgagaaagtc ttgtgagcca ccttgggcag cgagcggcgg gggtctatgc cggcgagcgc
     2401 ggcggcgcgg cgggccgggc gggtccctgc gagggcgttg tagagggaag gggagagcgc
     2461 ggcgagggcg agctgtctcc tgatgtggcc tgcggctttc tggcggacgc tgaagccgtg
     2521 cgttttaccc atctgggaga ggacctcggt cttcatgctc gccacgtcta cctgggaggg
     2581 gcactcggtc ttgcaggcct tgcagccgac gcagaggtcc atgacctcct ccatgcgttc
     2641 gcccgtgagc tcctcgggcg ggagcgtgcc gtctataacg gagcggagca agttggcgcg
     2701 ggcacgcgtg gtgtccttct cgtcgagggt gaccatgtac gaggggcaca tcgtgccgcc
     2761 ggtcttcttg cggcagaagc cgctcccgtt gcacaactcg acggcggccg cgaagttccc
     2821 ctgatgcgag aaatccaggt tcgtgtttat ggagatgcgt ctgcgaccgg ggccgatgcg
     2881 gagctgccgg tccatcggcg ggggagccac gatcacgccc gggttgagca tgccttccgg
     2941 gtcgaagagc gccttcacct cggagaaggc gcgcaggatc tcgggcccgt acatcctctc
     3001 caggaattcc gaacgggcga gaccgtcgcc gtgctcgcca gagagcgagc cgccgcagtc
     3061 cacgacgagg tcggcgaccc cttcggcgat gcgccgcatc ctcgccaccc cgtcggggtc
     3121 ggaggtgtcg agcgccggcc tgacgtgcag gcacccgacg ctcgcgtggc cgtagaagca
     3181 ggcccaggtg ccgttcttct ccactatctc ctcgaagcgt ccgataaact cctcgagccg
     3241 gtccggcggc acagccgcgt cctccacgaa tgcgacgggc ttctccctct ccgtagttcc
     3301 aagcaagagc ggcagtatgg acttgcggag cttgacggtc gcctgcatct cacccttggt
     3361 gcgcagcggc gtcgaatgga gcgccccgac ttcgcgggcc atctcttcga gcccggcaaa
     3421 ccgttcgtcc agctcctccc gcgtaccact ccactcgacg agcaggaggg ccttcgggtc
     3481 cccctgcacg aagcgggtgg agccggcgta ggctgtcgaa gcccgagccc ggccgatggc
     3541 cacgtcgtcg agcatctcca gggccgaggg gccgagcttc aacagctcga cggtcgcgcg
     3601 ggcggcggct gccagactgt cgaactccag ggaggcgagt gccctccgcg gcggaagctc
     3661 ggccaggcgg aagtcggcgc gggtcacgac cgccagagtc ccctccgagc cgcacaccag
     3721 gcgggtgaga tccacggtct ccgggtcaac gagggcgtcg agcccgtagc ccgagacgcg
     3781 ccggatcatt cgcgggaacc gcctctttat ctcgtcctcg tagcgctcct cgatctccag
     3841 agcgccccgc aggatcctcg aggcggcgtc ggcgtggctg gcgcgccgct tcgcctcctc
     3901 ccgcgagaga gacctcagct cgaccgtctc gccgctcgcg agcacgcagc gcagagacag
     3961 cacttgatct gccgtcgtgc catagacgat cgagcgcata ccggctgagt tgttcccgac
     4021 catgccgcca agagtggcga cgtcggaggt cgaggtatcg gcgccgaaga cgaggccgtg
     4081 cggctcgacg agggcgttca gctcgccctg tatcgtcccc ggctcgacag agcaacgctt
     4141 ctcctcgaga tctagagcga ataccttctg catctctgag aggtcgaggg cgaggccggg
     4201 ggcggtcgcc tgtccggcga ggctcgtacc agtgccgcga ggagttatag ggagcccgag
     4261 ctcgcgcgct gcggtgagcg cgagcttcac gtcctcctcc gagcgagcaa cgacgacgcc
     4321 gacgggtctg cgcaggtaga tcgaggcgtc cgtggaccac agggcgcggg aagcctcgtc
     4381 ggtgcgcagg tagccttcca ggcggccgtc cagagcgtcg cgcagccgct ccgcaagagg
     4441 ctcgttcctc tcccccatca tgcgcgaagt atagtctgcg ggcacattcc cagccaggag
     4501 tacaatccag gatgccgcgg aggaggttac ggctggaccg gctccggatc atgagcttca
     4561 acgtgcgggg gtcattcaga gactcgggca cccccaacgc ctggccggaa cgagccgagc
     4621 tgaacgtcga gacgatcagg caccacaccc ctgacgtgat cggcttccag gaactacaga
     4681 gcggcaatct ggagacctac ggggaaaccc tgccgcgata cgaccaccta ccgggaccgg
     4741 agtacggcaa tgcggcgcgc ccgagcctca acgcgatctt cttcgacgcc ggacgcctgc
     4801 gcctgctcgg cgcgggcggc ttctggctga gcatgacgcc ggagcgatac tcttcgagct
     4861 gggactcgag ggccgtccgg tcagccaact gggcgtattt cgagccggct ttcgccggcc
     4921 cgtccttcct gcacctgaac acgcacctcg accacgtgag cgagcgagcc cgcgtggagg
     4981 gcagccgcct gattattcgc aaagtagcga gactactgca gcgttacgga gaggacacgc
     5041 cggttctcgt cacgggggac ttcaactgcc gcccccggaa ccctgcgtac cgcagcttcg
     5101 tggagaacgg cttcgtggac acctacctcg ccgcaagcaa tgaggacgca ggcggcgcgc
     5161 acaccttcca cgccttcaag ggctcgcgct accgggacgc gcacccccac ctcggtccga
     5221 gacggattga ttggatcctg ctcaaagacc cactccatcg cctccgagtg gtctcgcact
     5281 atatcgtgcg ggatcacgcc ggggaaaggt acccgagcga ccactacccg gtgctcgccg
     5341 acctggcgct ctgaagagat atctggacaa acgaaagaga gagccccgaa acgggactcc
     5401 ctcttcgcaa gctcggtttt gctcaggccg cgttggagaa ggctgctccg aactcgccgt
     5461 tggcgagcat gcgctcggcc tcgcgctgga gctgtctgac ccgctcgcgc gagatgtcga
     5521 gctcgtcgct gagctgggcc agcgtcgcca cgtcgcgatc gtcgaggcca taccggcgga
     5581 gcaggacgta ccgatgtcgc tctggca
//
LOCUS       DASTUV010000068         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1350139, whole genome shotgun sequence.
ACCESSION   DASTUV010000068 DASTUV010000000
VERSION     DASTUV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350139"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFT03_01530"
     CDS             complement(<1..790)
                     /locus_tag="VFT03_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4846850.1"
                     /translation="MDTASTGNLLEVNGAKIYHEVRGSGPSVLFVAGATGDGGHFERV
                     AEILSDEFTVVTYDRRGNSRSPRPDGWESTSTEEHSEDAAELIEALGIAPVAIFGSSA
                     GAIIGLDLAIRHPELVRDAILHEPPMTAGMANPEEVMVAIQQVVEGGMQRGGLSGGVE
                     AFCRFVDGDEAFENLDSQLRERVLRNGETCLVTEMPVFPAYAPDEATLAAVEVPVRVM
                     VGPDSALGEPAGWLAGQLGVELEILPGGHTPYFDRPQEMAEALRP"
     gene            949..1575
                     /locus_tag="VFT03_01535"
     CDS             949..1575
                     /locus_tag="VFT03_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4846851.1"
                     /translation="MFEKMARSGKRGKYNLKARAERYQQTRQRIVEATLELHRTAGPA
                     QTTITEIAKKAGVERQTVYNHFPDELSLFKACSAHNRSLNPPPDPEPWKLIPDPEERL
                     RRALAEVYAYYRRNEQMLANVTRDAQANPNTRKVLEPRVKHQERMRDVLAAGWEQGDK
                     QRRKKFLYGSLWMALEFQTWHTLARQQGFANDEMVELMVGMVRCLMLD"
     gene            complement(1740..2579)
                     /locus_tag="VFT03_01540"
     CDS             complement(1740..2579)
                     /locus_tag="VFT03_01540"
                     /inference="COORDINATES: protein motif:HMM:NF005395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4846852.1"
                     /translation="MRDVIVVIGPGQIGQAIARRVGVGKHVLLADVRQENADVAAGVL
                     GNAGYEASVATVDVSSREAVHALVETATGLGDVSGLIHAAGVSPTQASPATILEVDLY
                     GTALVLEEFGNVIAPGGAGVVIASQSGHRLPPLTVEENKALATTPVEELLGLPFLQPD
                     RVTDSLAAYQLAKRGNSLRVMAEAVRWGERGARVNTISPGIVMTPLAKDELGGPRGAG
                     YRRMIEGSAAGRAGTPDEVGTVGALLMGPDGGFITGSDFLMDGGVTAAYWYGDLAEVR
                     AQQ"
BASE COUNT          527 a          916 c          852 g          497 t
ORIGIN      
        1 tcggtcgtag agcctccgcc atctcttggg gccggtcgaa gtagggggtg tgtccgccgg
       61 gaaggatctc caactcgacg cccagctggc cggcaagcca ccctgccggc tcgcccaacg
      121 cgctgtcagg gcctaccatt acccgcacgg gcacctcgac ggcggcgagc gtggcttcgt
      181 caggcgcgta agccggaaag accggcatct cggtgaccaa gcaggtctcc ccgttcctta
      241 gcaccctctc ccgcagctgc gagtctaggt tctcgaacgc ttcgtcgccg tcgacgaagc
      301 gacagaacgc ttctacccct ccactaaggc ctcccctttg catgccgccc tccacgacct
      361 gctgaatcgc taccattacc tcctcgggat tggccatgcc tgccgtcatc ggcggctcgt
      421 gcaggatggc gtcccgcacc aactcggggt gacggatcgc gaggtccagg ccgatgatgg
      481 cccccgcgct gcttccgaag atggcaacgg gggcaatccc taaggcctcg atcaactcgg
      541 cggcgtcctc cgagtgctcc tcggtcgagg tactctccca tccgtcgggg cgggggctgc
      601 gcgaattgcc gcgccgatcg taggtgacga cggtgaactc gtcggagagg atctcggcca
      661 cccgctcgaa gtggcctcca tccccagtag cgcccgctac gaagagcacg gagggtccgc
      721 ttccgcgcac ctcgtggtag atcttcgcac cgttgacctc gaggaggttg cccgttgacg
      781 cggtatccat agccatcttt ccctttcatg tcggtgttcg aagcttcgac gcccttctcg
      841 taagaacgtt cttgtagaag ccctccttcc tctaataaat atgcagcgtg tctaacaatt
      901 aacggcatat tacggtatga ttagacgccc tgtcaagaga gaaaggggat gttcgagaag
      961 atggcacgga gcggcaagag aggcaagtac aacctaaaag ccagggccga gcgctaccag
     1021 cagacgcgcc aaaggatcgt cgaggcgacc ttggagctgc atcggaccgc tggacctgca
     1081 cagaccacca taaccgaaat agccaagaag gcgggggtgg agcgccagac cgtctacaac
     1141 cacttccccg atgaactctc cttgttcaag gcctgctccg cgcacaaccg ctccctcaat
     1201 ccgcctccgg acccggagcc ctggaagctt attccggatc cggaggagcg cctgaggagg
     1261 gcgctcgccg aggtctacgc ctactaccgg cgcaacgagc agatgctggc caacgtaacc
     1321 cgggacgcgc aggcgaaccc caatacgcgc aaggtgttag aaccccgcgt aaagcaccag
     1381 gagaggatgc gtgatgtcct cgccgccggc tgggaacaag gagacaaaca gcgccgcaag
     1441 aagtttctgt atgggtccct gtggatggct ctagagttcc agacgtggca cacgcttgcg
     1501 aggcagcagg gattcgcaaa cgatgagatg gtggagctga tggtgggcat ggtgcgatgc
     1561 ctgatgctcg actagcggtc tctatttacc caacctcgtg gaaggggtgt tgcgaacttc
     1621 gcatgtacgg agttctgaga agttgatacc gatgctctcc gcttgcgacg cgggtgctgg
     1681 ttactccccg ggcgtcttga tcggcccggc cagatgtgct caaccggtct gttggacgct
     1741 cattgctgtg cgcgcacttc ggcgaggtcg ccgtaccagt aggcggcggt caccccgccg
     1801 tccatgagga agtcgctgcc ggttatgaac cccccgtcag ggcccatcaa cagcgcgccg
     1861 accgttccca cctcgtctgg agtgccggcg cgcccggccg cggacccctc gatcatgcgc
     1921 cggtaccctg cgccgcgcgg accgccgagt tcgtccttgg ccagcggggt cataacgatg
     1981 cccgggctga tcgtgttgac ccgcgcacca cgctcgcccc agcgaaccgc ctcggccatc
     2041 acgcgcagcg agttgccgcg cttcgcgagc tggtaggcgg ccagggagtc cgtcactcgg
     2101 tcgggttgaa ggaaaggaag tccaagcagt tcctccacgg gcgtcgtggc caacgccttg
     2161 ttctcctcaa cggtgagcgg gggaaggcga tgcccagact gcgacgcgat cacgacgccc
     2221 gcgccacctg gggcgatgac gttgccgaac tcttctagca cgagcgccgt gccgtacagg
     2281 tcgacctcga ggatcgtcgc cggtgacgcc tgcgtgggtg agacgccggc agcatggatc
     2341 agcccactga cgtcgcccag acccgtggcc gtctcgacga gcgcatgcac ggcctcgcgc
     2401 gaggacacgt cgaccgtcgc gacgctcgct tcgtatccgg cgttccccag gacccctgcc
     2461 gccacatcgg cgttctcctg gcgcacgtcg gccagcagga catgcttacc gacgccaacc
     2521 cggcgcgcga tcgcttgacc gatctggccg ggtccgatga ctacgattac atctctcata
     2581 gctattccct tcttcggaag atcgcaccac agtcggtgcg actagtatgc cgctgggacg
     2641 acggcgataa acagtcgatc catagaaccg gtaggcacac gaagttatgg cacgcgaggg
     2701 agtcaagcaa ccggggccga tccatgggta gctattcacc ctactgcgtg cagacctcga
     2761 cgctcctatg tttgtcaaca aggccggaga ga
//
LOCUS       DASTUV010000069         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_639982, whole genome shotgun sequence.
ACCESSION   DASTUV010000069 DASTUV010000000
VERSION     DASTUV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639982"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFT03_01545"
     CDS             <1..782
                     /locus_tag="VFT03_01545"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEU4846853.1"
                     /translation="PGVAAVHRQSDTLLGGEVTLLVDEPNPRPFPGYYYEPHELRKTF
                     AEKDWKKVVGFQTRNPVHRAHEYIQKSALETVDGLLLNPLVGETKSDDISAAVRMMSY
                     EVILDRYYPRGRTVLAVFPAAMRYAGPREAIFHAVCRKNYGCTHFIVGRDHAGVGSYY
                     GTYDAQHIFEEFDEDELGITPLFFEHAFFCLNCEGMATTKTCPHGKDAHVFFSGTKVR
                     EMLGRGEYPPPEFSRPEVVKVLIEGMRDEERLIKQPRAFQE"
     gene            813..1385
                     /gene="cysC"
                     /locus_tag="VFT03_01550"
     CDS             813..1385
                     /gene="cysC"
                     /locus_tag="VFT03_01550"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEU4846854.1"
                     /translation="MENGSRYGRGFTLWFTGLSGAGKTTIADIVERELRLRHGKVEVL
                     DGDIVRTNLSKGLGFSREDRDTNVLRIGFVADLLTRNGVGVIVSAISPFKEVRDQVRR
                     NVGEDFIEVFVDAPLEVCAERDVKGLYEKAFSGEIPQFTGVSDPYEPPTAPELHIKTN
                     EEEPRESARRVIARLEEFGYLRAVREFDRA"
     gene            1388..2185
                     /locus_tag="VFT03_01555"
     CDS             1388..2185
                     /locus_tag="VFT03_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4846855.1"
                     /translation="METTPTLFGLLVGFLVGLTGVGGGSLMTPFLIAVMGVSAPTAVG
                     TDMVFATLTKFTGSLQHYRQRSVNLEIAVFLGLGSIPAGLIGVATLEWIEGSYDAETV
                     KSIMITIIAITLVLVGASLIFRNFMPERKEGPKPGVWDGKGRMSNRRRAYTVLFGAMG
                     GYLVGLTSIGSGSIMAVILLLLYPIAPAVIVGTDIAHATVLSLVVGIAHMTQGNVDFS
                     LVGSLLLGGVPGVLVGSRLAPWLPGKPLKMILAVMLIFVGARLLLAG"
     gene            complement(2191..>2610)
                     /locus_tag="VFT03_01560"
     CDS             complement(2191..>2610)
                     /locus_tag="VFT03_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU4846856.1"
                     /translation="LTLLALLLVAAILLLVLPFAGDRANESVPNEKASGDVAVARGPV
                     AGGEAQGAEADPETRDQEESNPGERPDVSGKSVVEAARVLSEGGYEVAAIRTVKSDEE
                     AGMALRVEPAAGSTVEPGASVVLVTSGGPTGRETRSF"
BASE COUNT          483 a          853 c          799 g          475 t
ORIGIN      
        1 atccaggcgt ggccgccgtg caccgtcaga gcgacacgct cctcggaggc gaggttacgc
       61 tcctcgtcga cgagcccaac ccacgtcctt tccccggcta ctactacgaa ccgcacgaac
      121 tcaggaagac gttcgcggag aaggactgga agaaggtggt tggcttccag accagaaacc
      181 ccgtccatcg agcccacgag tacatccaga agagcgctct cgagacagtg gacggactgc
      241 tcttgaaccc cctcgtcggt gagaccaagt ccgacgacat ctcggccgcg gtcaggatga
      301 tgagctatga ggtcatcctc gaccgctact accctagagg caggacagtt ctcgctgtgt
      361 tccctgctgc gatgcgctac gcgggtcctc gtgaggcgat cttccacgct gtctgccgca
      421 agaactacgg ctgcacacac ttcatcgtgg gcagggatca cgcaggggta ggcagctact
      481 atggcaccta cgatgcccag cacatcttcg aggagttcga cgaggacgag ctcgggatca
      541 cgccgttgtt cttcgagcac gccttcttct gtctcaactg cgagggcatg gcgacgacca
      601 agacgtgtcc gcacggcaag gacgcgcacg tcttcttctc ggggacgaag gtgagggaga
      661 tgcttggcag gggcgagtat ccgccgcccg agttctctcg tccggaggtt gtcaaggtgc
      721 tcatcgaggg catgcgggac gaggagcggc tcatcaagca gcctcgcgcg ttccaggagt
      781 agtagaaaga gaaagcaaac aggagggtgg gaatggagaa cggaagcagg tacggtcgcg
      841 ggttcacttt atggttcaca ggtctctccg gggcaggcaa gacgaccata gcagacatcg
      901 tcgagaggga gctgcgccta aggcacggca aggtcgaggt actcgacgga gacatcgtac
      961 gcaccaactt gtctaagggc ctcggcttca gccgcgaaga cagggacacg aatgtattga
     1021 ggatcgggtt cgtggcagac ctccttaccc gcaacggggt cggggtcatt gtgtcggcca
     1081 tctcgccgtt caaggaggtc cgcgaccagg taaggcgcaa cgttggggag gacttcatcg
     1141 aggtcttcgt cgacgcgccc ctggaggtct gcgccgagcg tgacgtgaag ggcctctacg
     1201 agaaggcgtt ctctggtgag atcccgcagt tcacaggcgt ctccgacccc tacgagccgc
     1261 ccaccgctcc cgagctccac atcaagacca acgaggaaga gccccgcgag agcgcgagaa
     1321 gggttatcgc caggctcgag gagttcggct acctccgggc cgtcagggag ttcgaccggg
     1381 cgtaaagatg gagactaccc ccaccctctt cggcctcctg gtcgggttcc tcgtcggcct
     1441 cacgggggtg gggggcggtt ccctgatgac gccgttcctg atcgccgtga tgggcgtctc
     1501 tgccccaacc gcggtcggga cggacatggt cttcgccacc ctcaccaagt tcaccggttc
     1561 gctacagcac taccgccagc ggtcagtgaa cctcgaaata gcggtcttcc tcggcctcgg
     1621 gagcatcccg gcggggctca taggcgtggc gactctggag tggatcgagg gctcctacga
     1681 cgccgagacc gtgaagtcga taatgatcac gatcatagcc atcaccctcg tgctggtcgg
     1741 ggcgtccctg atctttcgca acttcatgcc agagcgcaaa gagggcccga agccgggcgt
     1801 ctgggacggc aagggtcgta tgagcaacag acgccgcgca tacacggtgc tcttcggcgc
     1861 catgggcggc tacctcgtgg gtctcacctc tataggctcg gggagcatca tggcggtcat
     1921 actcctgctc ctctacccga tagctcccgc cgtgatagtc gggacggaca tcgcccacgc
     1981 gaccgtgctc tctctagtgg ttggcatcgc ccacatgacg cagggcaacg tggacttctc
     2041 tctggtcggc tcgctgctcc tcggcggcgt ccccggcgtc ctcgtcggca gccggctcgc
     2101 tccctggctg ccaggcaagc cgctgaagat gatcctcgcc gtcatgctga tcttcgtcgg
     2161 agcccgcctg ctcctcgcgg gataaccctg ctagaagctc ctggtctccc tgccggtcgg
     2221 tccgccactc gtaactagca ccacagaggc tcccggctcc actgttgacc ccgccgcagg
     2281 ttctaccctc aacgccatgc cggcctcttc gtcgctcttc acggtccgta tggcggccac
     2341 ctcataccca ccttccgaga ggacccgcgc ggcctcaacg acgctcttgc ccgagacgtc
     2401 cggtctctct cccggattcg actcttcttg atcccgagtt tccggatcag cctccgctcc
     2461 ctgcgcctcg ccgcccgcca caggaccccg agcgactgcc acatcaccgg aagccttctc
     2521 gttgggcacg ctctcgttcg cccggtcgcc cgcgaacggg aggaccagaa gcaatatggc
     2581 tgccacaagc agcagcgcga ggagcgtcag
//
LOCUS       DASTUV010000070         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1350119, whole genome shotgun sequence.
ACCESSION   DASTUV010000070 DASTUV010000000
VERSION     DASTUV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350119"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFT03_01565"
     CDS             <1..216
                     /locus_tag="VFT03_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846857.1"
                     /translation="ETERQIAAAEIDIKRLDAERELEMIELQMGLRDADSLEPAPTEE
                     ANEEAPAEQPDEAPAEGPPEEEERSRG"
     gene            209..997
                     /locus_tag="VFT03_01570"
     CDS             209..997
                     /locus_tag="VFT03_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846858.1"
                     /translation="MARKQSGSQILMRLAPFGAFTGFGTLAFIGDAYTLAALLGVGAF
                     VTGSRLLDRERTRRKELWRRVRDNERELGRVAREDRVATPQMKRLISLQRGLLESWEL
                     LPEEYRPLLDGDVSSIVGEVEGTARLARRRAALRRHLDSIDRSQISRRIEGLERDLAE
                     LEEGSPLRAPFETALSGRRGELASCADILDGISRINAQLEGAESLLGNLRGELLALDT
                     SLSPGSLESSLVHLKERVAYFRQSLDEVTRSVETLPATERLPVR"
     gene            994..1674
                     /locus_tag="VFT03_01575"
     CDS             994..1674
                     /locus_tag="VFT03_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846859.1"
                     /translation="MSEERPATRGGGFFSRVFSGNLEGEDETTIYPAREVGVEETGEE
                     TVETRGFTVERAAEIIKHLPPEVPRPAAVRIVRGTLEAAGINVDDLARSTGTREAKLN
                     SEIELSQDRIQEFKDRTEEVIRTYEDEIRKAREARDFGISEEERKISASRSGLEDVER
                     VRDFFGLSGEPQPAAEDPAGSSTTSDLPPGEETQVMEPADEDDTQILRRPGPLSDNYF
                     ETGENRDR"
     gene            1671..2135
                     /locus_tag="VFT03_01580"
     CDS             1671..2135
                     /locus_tag="VFT03_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU4846860.1"
                     /translation="MSISRRLGRIARGLVTNLQEDERFRETLRAGRERGEALRGAFEA
                     GLRGASEEWRAAERRRAAEERHTEAGERPSGGWSSAFPPRRYQPNVLAAYNRLGLVPG
                     ASIEEADKKRRELIKRYHPDRFSEPEQRARAERITAEINAAHDTIERHLLRR"
     gene            2197..3030
                     /locus_tag="VFT03_01585"
     CDS             2197..3030
                     /locus_tag="VFT03_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4846861.1"
                     /translation="MRVDGMIEEFSAYLSDDPNTSPRTAEFYEADLKEFARFLAEESV
                     TEVEDVSVGAVMEFRNRLLDERRKRFTVYRKDAAVRRFLDWVRTSTDAELDFDPIEPM
                     HQPLDQQIVFLEEEEAGQLLSFPIKARDDARDAAVIRLMLDTGVTVSEIRGLLRSDVD
                     LEAGQIQLGGPGSHLAPRALRLSAATVEAVRRYLDMRHDETPELIVGRAARPIATSKS
                     LQNSIWKRCEEVGMWRVSPMVLRHTFAIRLMRRGATLNELREALGVRDTSNIGVYRKF
                     V"
BASE COUNT          664 a         1064 c         1164 g          517 t
ORIGIN      
        1 gagaccgagc gccagatagc cgccgccgag atagacataa aacgcctcga cgccgagcgc
       61 gagctcgaga tgatagaact ccagatgggc ctgcgggacg ccgactcgct cgagcccgcg
      121 cccaccgaag aggccaacga agaggccccc gccgaacagc cggacgaggc gccggccgaa
      181 ggcccgcccg aggaggaaga gagatcccgt ggctagaaaa cagagcggct cgcagatcct
      241 gatgcgcctg gcccccttcg gggcgttcac cgggttcggg acgctggcgt ttataggaga
      301 tgcttatacc ctcgccgcgc tcctcggcgt cggagcgttc gtcacgggca gtcgtctact
      361 ggatcgggag aggaccaggc gcaaggagct gtggcggcgg gtgcgggaca acgagcggga
      421 gcttggcagg gtggcgcgcg aggaccgggt agctacgccg cagatgaagc ggctcatctc
      481 cctgcagagg gggctactgg agagctggga gctgttgccg gaggagtaca ggccgctgtt
      541 ggacggggac gtctccagca tcgtcggcga ggtagagggc acggcgcggc tagccaggcg
      601 gcgggctgca ctcaggaggc acctggacag catcgaccgg agccagatct cgcgccgcat
      661 cgagggattg gagcgggacc tggcggaact ggaagagggc tcgcccttga gggcgccgtt
      721 cgagaccgct ctctccggga ggcgcgggga gcttgcctcg tgcgcggata tcctggacgg
      781 tatcagcagg atcaacgccc agctcgaggg cgcggagagc ctgctcggca acctgcgcgg
      841 ggagctgctc gccctcgaca ccagcctctc cccgggctcg ctcgagtcga gcctcgtgca
      901 cctcaaggag agggtcgcgt acttccgtca gagccttgac gaggtgacgc gaagcgtcga
      961 gaccctgcct gcgacagaga ggctgcccgt acgatgagcg aggaacggcc ggctacgcga
     1021 ggaggggggt tcttctccag ggtgttctcc gggaacctgg aaggcgagga cgagaccacg
     1081 atctacccgg caagggaggt cggtgtggag gagaccgggg aagagacagt ggagacgcgg
     1141 ggtttcaccg tcgagcgggc ggccgagatc ataaagcacc ttccgccgga ggtgccgcgc
     1201 cccgccgcgg tgcgtatcgt gcgggggact ctggaggccg cgggcataaa cgtcgacgac
     1261 ctggcgagat ccacagggac gcgggaggcc aagctcaact ccgaaataga gctcagccag
     1321 gaccgcatac aggagttcaa agacaggacc gaggaggtga tccggaccta cgaggacgag
     1381 atccggaagg cccgcgaggc cagggacttc ggtatctccg aggaggagcg caagatctcc
     1441 gcctcccgtt ctggcctgga ggatgtcgag agggtgcgcg acttcttcgg tctgtccggg
     1501 gaaccccaac cggccgcgga ggacccagcc ggctcctcga cgacgagcga cctccctccc
     1561 ggggaggaga cgcaggtgat ggagcccgcg gacgaggacg acacccagat cctgcgccgt
     1621 cccggcccgc tctccgataa ctatttcgag accggggaga accgcgatcg gtgagcatct
     1681 cgcgccgcct cgggcgcata gcccggggtc tcgtaaccaa cctccaggag gacgagcgct
     1741 tccgcgagac cctgcgcgcc ggccgtgagc gcggcgaggc gctcagagga gccttcgaag
     1801 ccggactgag aggggcctcc gaggagtggc gcgccgccga gagacggcgg gccgccgagg
     1861 agcgtcacac cgaagcaggg gagagaccat cgggcgggtg gtcctccgcc ttcccgccca
     1921 ggaggtatca acccaacgtc ctcgcggcct acaaccggct cggcctggtg cccggcgcct
     1981 ccatagaaga ggcggacaag aagcgccggg agctcatcaa gcgctaccac cccgaccgct
     2041 tctccgagcc cgagcagcgc gccagggcgg agcggatcac agccgagatc aacgccgctc
     2101 acgacacgat cgaacgccac ctccttcgtc gctaggtttc agattgtggt ttttcctgat
     2161 gcctgaagcc tcgcgtttgc cgttagaata gggttcatga gagtagacgg tatgatcgag
     2221 gaattctccg cctacctctc cgacgacccg aacaccagcc cgcgcaccgc ggagttctat
     2281 gaggcagatc tcaaggagtt cgcacgcttc ctcgcagagg agagcgtcac ggaggtcgaa
     2341 gacgtgagtg tcggcgccgt gatggagttc cgaaaccgcc tgctagacga gcggcgcaag
     2401 cggttcaccg tctaccgaaa ggacgctgct gtgcggcgct tcctcgactg ggtgaggact
     2461 agcaccgacg ccgagctcga cttcgacccg atagagccta tgcaccagcc actcgaccag
     2521 cagatagtct tcctcgagga agaagaagcc gggcaactcc tctccttccc gataaaggcg
     2581 cgcgacgacg cccgtgacgc cgccgtcata aggctgatgc tcgacacagg ggtgacggtc
     2641 agcgagatcc ggggcctcct gaggagcgac gtggatctcg aggccggcca gatacagctc
     2701 ggcggcccag gctcccacct cgcaccccgc gccctccgcc tctccgcggc gacggtcgag
     2761 gccgtcaggc gctacctgga catgcgtcat gacgagaccc cggagctgat cgtgggccgc
     2821 gccgcccgcc ccatcgcgac ctccaaatct ctccagaact ccatctggaa gcgttgcgag
     2881 gaggtgggca tgtggcgcgt ctctccgatg gtcctccgcc ataccttcgc catccggctc
     2941 atgaggcgcg gcgctaccct gaacgagctc agggaagctc tgggtgtccg cgacacctcc
     3001 aacatcgggg tgtacaggaa gttcgtctag cttacgcgcc aggccgtcct ttggcatacg
     3061 ttgatctcga attctggggt tagatacctc tgaatgggtg tcctaaagag ctctactgtc
     3121 gtcccttata gtggtgtgca ctttggctga ggcacaagat gtaggcggcg ctaagtgcgg
     3181 cccgagtcca gagtacccag agtgaatgag ccgggattca agcttgtgta gccagcgcgc
     3241 cgcaccgacg ggttttattc cgggcgtggt gggtagagaa ggtcatcgat aacgtaagac
     3301 tcgactaggg aggatgcaca gaaatgagct tgggcaactt gggcgacctt ggcaacctgg
     3361 atcttgacca actcctacag tacctgccga acctggactt tccggctga
//
LOCUS       DASTUV010000071         6246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_814381, whole genome shotgun sequence.
ACCESSION   DASTUV010000071 DASTUV010000000
VERSION     DASTUV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814381"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFT03_01590"
     CDS             <1..833
                     /locus_tag="VFT03_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846862.1"
                     /translation="GALWPATRAAADAAEASLGADGLPPGGADYWEVETWRPNLGTAA
                     PLRTGLRAAADLARRLGHESDARRYAAAAGRLDAGLACEFAPAGYRRTPYPRSGADAA
                     VNFLAPPFAPPDTRIEEAIAETAERLRAPNGGVLPGERWPQDPTVAWTPETAFFALSA
                     AASGDGEEAGRWLGWLAAHRTSLGAFPETVDGDGNPRAAAPLGWTEAIVLLALAAKDE
                     PLPTPPVPERVPPETSTPQLPPVIGALLCAGVLAAFVAAGRRELTPPRWRPGPGSGRL
                     "
     gene            complement(787..1245)
                     /locus_tag="VFT03_01595"
     CDS             complement(787..1245)
                     /locus_tag="VFT03_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU4846863.1"
                     /translation="MKGVVEGIFVTSEGSAALERVEEVRTIEGCGIEGDRYCEGTGFW
                     TRYGDVCQVTLIEGEDLDYIEDELGISVKGGEHRRNIITRGIRLGDLRRKRFRVGEAV
                     LEYDRPRPPCRHVQELSEPGMTRALRGRGGICARVIKGGRIQARDAIEVE"
     gene            complement(1313..2866)
                     /locus_tag="VFT03_01600"
     CDS             complement(1313..2866)
                     /locus_tag="VFT03_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU4846864.1"
                     /translation="MSETRLDPVTLSVLASALAGIAEEMGAVLIRGSYSSNIKERRDC
                     STALFDAKGKMVAQAEHIPVHLGAMPEAVAAIMRREPEPGDVFAINDPYSGGTHLPDI
                     TLVSPVAYEGEILGYAVTRAHHSDVGGMRPGSMPSDSREIFQEGIIIPPVRLVRGGEY
                     EDDVLDLLLANVRTPDLRRGDLRAQIAANNIAEERIGELIERRSKDVILAAFEEVVSY
                     AERRTREAIRELPDGEHEAEDFMEGDGTVDEDIPIRATVTIHGDSMTIDFAGTSGQVR
                     GNINCPLPVTRSACYFALRVLLPKDILANAGTYAPLEIKAPEGSLVNATYPAAVVAGN
                     VETSNRIADTVLAALSGFAPEDVPAQGQGTMNNTIIGGSGNGGWTYYETIGGGQGASS
                     KGPGPSGVHIGMSNTLNTPIEAFELEYPMRVERYELRYGSGGAGEYRGGDGIERSVKV
                     LEPASLSLLTDRRRHPPRGIEGGEPGQVGENLLNGEELPPKVGRELEEGDVVTVRTPG
                     GGGYGRARE"
     gene            complement(2863..4833)
                     /locus_tag="VFT03_01605"
     CDS             complement(2863..4833)
                     /locus_tag="VFT03_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU4846865.1"
                     /translation="MSETRLGVDVGGTFTDLVALSDGTLVTAKVPSTPRDQSEGVMNA
                     VRTSEVEPGAVSALAHGMTVATNALLERRGARIALVTTEGFRDVLEIARQNRASLYDL
                     AADRPPPLVPRELRFTVTERMGPEGEILPLDDESLMAAVSAVEEAEVEAVAVCLLFAF
                     AHPEHEERVGEALREALPDVYVSLSSEVLPEFREYERFSTTAADAYLAPRLAAYLKNL
                     AGKAEESGVPPPLIMQSSGGVARIDDAIEDAAGCVLSGPAGGVVGAAYVGALGGYQDL
                     LTFDMGGTSTDVAPIIGGEAQTTTETVVAGVPIKLPMVDVHTVSAGGGSIAWADAGGA
                     LRVGPRSAGAEPGPAAYGMGGEEPAVTDANLYLGYLRDGAELGGEVVLQRDLAEEALD
                     NLGERLGLDVQETALGIVRVANAEMVRALRVISVERGLDPREFALLAFGGAGGMHACS
                     LAEELGIETVLVPRAGGVLSALGLAISDLRHDYVRPYLAPLAEVDAAEFEERFEGMEK
                     IASDELEAPEFTRRADLRYTGQSFELTVEADPLEKLEERFHAAHEQRYGYRMDDEPVE
                     LVNLRLIATVPVEKPGLDEPAAEDETVSERREANFDSEWLEVPVYDRESMGEGSEVEG
                     PAIIEFKESTCVVRPGWRGAVDGVGTLVLERQ"
     gene            complement(4838..6175)
                     /locus_tag="VFT03_01610"
     CDS             complement(4838..6175)
                     /locus_tag="VFT03_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4846866.1"
                     /translation="MQRVGGEDTGVPSSIRKVVVSSFIGTTIEWYDYFIYGTAAALVF
                     PALFFPDFSELAGTLLSFSTFAVGFAARPLGGIIFGHFGDRIGRKAMLVTTLLIMGVA
                     TFLVGCLPSFDQIGVWAPILLVVLRLTQGLGLGGEWGGAVLMSVEHSPPEQRGLNGSW
                     PQMGAPAGLVLATGVFAAVSAITGDAFEVWGWRVPFLLSIILIGVGLFIRLRIYESPA
                     FSQVRESGTEARMPIIDVLRTYPKNVALGVGSRIGIDAAFYILAVYSLTHISANLGLS
                     PNIGLLAVSIAALIEIFTIPSFGSLSDRMGRKPVLIGGAALLGIWTFPFFGLLNTKST
                     ILIILATIVGLSIAHAAVYAVQGSFYAELFGTRVRYSGASFSYQFSGIFGGALAPLIA
                     TWLYPQGGTTLIAAYVAALCALSVVCYALAQETYRKDIYAEDPQERRLVAEQG"
BASE COUNT          940 a         2177 c         2080 g         1049 t
ORIGIN      
        1 caggggcgct ctggcccgcc acgcgcgccg cggcggacgc ggcggaggcc tcgctcgggg
       61 ccgacggtct gccgcccggc ggcgcggact actgggaggt cgagacctgg aggccgaacc
      121 tcggcacggc cgctcccctg agaaccggcc tgcgcgccgc cgcggacctg gcgcgcaggc
      181 tcggccacga gtctgatgcc aggcgttacg cggcggcggc cgggcgtctc gacgcagggc
      241 tagcctgcga gttcgccccg gcgggctacc ggcgcacccc ctacccccgc agcggcgcag
      301 acgcggcggt caacttcctc gccccgcctt tcgcgccgcc cgacacccgt atcgaggagg
      361 caatagccga aacagcagag cggttgcgcg cccccaacgg cggcgtcctg ccgggtgaga
      421 ggtggccgca ggacccgacc gtcgcctgga cgcccgagac tgccttcttc gccctctcgg
      481 ctgcggcttc cggcgacggg gaggaggccg gccgctggct gggctggctc gcggctcacc
      541 gcacgagcct gggcgctttc ccggagacag tggatggcga cggcaaccct agagccgccg
      601 ctcctctggg ctggaccgag gcaatcgtgc tgctcgcgct ggccgcgaag gacgagccgt
      661 tgccaacgcc gcccgtcccg gagcgggtgc ccccggagac gagcacgcct cagctcccgc
      721 cggttatcgg ggcgttgctg tgcgccggtg ttctggctgc gttcgtcgct gctggaagaa
      781 gagaacttac tccacctcga tggcgtcccg ggcctggatc cggccgcctt tgatcacccg
      841 cgcgcagatc ccgccgcgcc ccctcaaagc ccgcgtcata cccggctcgc tcagctcctg
      901 gacgtgtctg cacggcgggc gcggcctgtc gtactcgagc accgcctccc cgacgcggaa
      961 cctcttgcgg cgcaggtctc ccaaccttat cccgcgcgtg atgatgttgc gccggtgctc
     1021 gccgcccttg acgcttatgc ctagctcgtc ttcgatgtag tcgaggtcct cgccctcgat
     1081 cagggtgacc tggcaaacgt ctccataacg cgtccagaac cccgtcccct cgcagtaacg
     1141 gtcgccctcg atcccgcagc cctcgatggt cctcacctcc tcgacgcgct ccaacgccgc
     1201 gctgccctcg ctggtcacga agatcccctc gacaacaccc ttcattccag cgctccttct
     1261 ggttcgacgg ctatgtgccc ggagtttgca gggtatacca ctgctcccgt tactactctc
     1321 gggctcgtcc gtaccctccg ccgcccggag tgcggaccgt aaccacgtcc ccctcttcga
     1381 gctcccggcc gaccttgggg ggtagctcct cgccgttgag gaggttctcg cccacctgcc
     1441 cgggctcccc accctcgatg ccgcgcgggg ggtggcgcct gcggtcggtg aggagggaga
     1501 ggcttgcggg ttcgaggacc ttcaccgagc gctcgatgcc gtcgccgccg cggtattcgc
     1561 ctgcgccgcc ggaaccgtag cgcagctcgt agcgctcgac ccgcatcggg tactcgagct
     1621 cgaaggcctc gataggggtg ttgagggtgt tggacatgcc gatgtggacg cccgagggac
     1681 ctgggccctt cgaggaggcg ccctggccgc cgcctatcgt ctcgtagtac gtccagcctc
     1741 cgttacccga cccgccgatg atcgtgttgt tcatcgtgcc ctggccctgc gcagggacgt
     1801 cttccggggc gaagcccgag agggccgcga ggacggtgtc ggcgatgcgg ttgctcgtct
     1861 cgacgttccc cgcgacaacc gccgcagggt acgtggcgtt caccaggctg ccctcggggg
     1921 ctttgatctc gaggggcgcg taggttccgg cgttggcgag gatgtctttg gggaggagga
     1981 cgcgcagggc gaagtagcac gccgagcgcg tcacgggcag cgggcagttg atgttgccgc
     2041 gcacctggcc cgaggtgccg gcgaagtcta tggtcatgga gtcgccgtgg atcgtgacgg
     2101 tcgctcttat ggggatgtct tcgtccactg tcccgtcgcc ctccatgaag tcttcggcct
     2161 cgtgctcgcc gtctggcagc tcgcggatgg cctcgcgggt acgccgctcg gcgtaggaga
     2221 ctacctcctc gaaggccgcg aggatgacgt ctttgctccg gcgttcgatc agctcgccta
     2281 tccgctcttc ggcgatgttg ttagcggcga tctgggcgcg caggtcgcca cggcgaaggt
     2341 cgggggtgcg cacgttggcg agcaggaggt cgaggacgtc atcctcgtac tcgccacccc
     2401 ttaccagacg cacggggggg atgatgatgc cctcctggaa gatctcgcgc gagtccgagg
     2461 gcatggagcc ggggcgcatc ccgccgacgt cggagtggtg cgcccgcgtg acggcgtagc
     2521 ccaggatctc tccttcgtag gcgacggggg agacgagggt tatgtccggc aggtgggtgc
     2581 cgcccgagta ggggtcgtta atggcgaaga cgtcgcccgg ctctggctct ctgcgcatga
     2641 tggctgcgac cgcctccggc atcgccccga ggtggacggg gatgtgctcg gcctgggcga
     2701 ccattttgcc tttcgcgtcg aagagggcgg tcgagcagtc tcgccgctcc tttatgttcg
     2761 aggagtagga gccccggatc aggacggccc ccatctcctc ggctatgccc gccagagcgc
     2821 tcgccagcac cgagagcgtt accgggtcca gcctcgtttc gctcattgcc tctccagtac
     2881 cagcgttccc accccgtcca cggctcccct ccagccgggc cgcacgacgc aggtggactc
     2941 tttgaactcg attatggcag ggccttctac ctcggagcct tcgcccatac tctccctgtc
     3001 gtacaccggc acctccagcc actcgctatc gaagttcgcc tcccggcgct cggatacggt
     3061 ctcatcctca gcagccggct cgtccagccc cggcttctcg accgggacgg tggcgatgag
     3121 gcgcaggttc accagctcca cgggctcgtc gtccatccgg tagccgtacc gttgctcgtg
     3181 cgcggcgtgg aagcgctcct cgagcttctc gagagggtcg gcctcgaccg tcagctcgaa
     3241 ggattgcccc gtgtagcgca ggtcggccct gcgggtgaac tctggagctt cgagctcgtc
     3301 agaggctatc ttctccatcc cctcgaacct ctcctcgaac tcggctgcgt ccacctcggc
     3361 gaggggcgcg aggtaagggc gcacgtagtc gtgcctgagg tccgagatgg ccaggccgag
     3421 agcggagagc acgccgccgg cgcgggggac aaggaccgtc tcgatgccga gctcctcggc
     3481 cagggagcag gcgtgcattc cgcccgcgcc gccgaaggcc agcagggcga actcgcgcgg
     3541 gtcgaggccg cgctcgacgc tgatcacgcg cagcgcgcgc accatctccg cgtttgccac
     3601 ccgcacgatg cctagagcag tctcctgcac atccagaccg agcctctctc ccagattatc
     3661 cagagcttcc tccgcgaggt cgcgttgcag tactacctcg ccgccgagct cggccccgtc
     3721 gcgcaggtac cccagataga ggttggcgtc cgtcacggcg ggctcctcgc cgcccatgcc
     3781 gtaggcggcc ggacccggct ccgcgccggc cgagcgaggg ccgactctta gcgctccgcc
     3841 cgcgtcggcc cacgctatgg aaccgccgcc ggcgctcacg gtgtgcacgt ccaccatggg
     3901 aagtttgata ggcacccccg caaccaccgt ctccgtcgtc gtctgcgcct cgccgccgat
     3961 gatgggggcg acgtcggtgc tcgtcccacc catgtcgaag gtcaggaggt cctggtagcc
     4021 gcctagagcg ccgacgtagg ctgcgccgac gaccccgccc gcgggtcccg agaggacgca
     4081 gccagctgcg tcctctatcg cgtcgtctat ccgcgccacc ccgcctgagg actgcatgat
     4141 caggggcggg gggacgcctg actcttcggc cttgccggcc aggttcttga ggtaggcggc
     4201 gagcctgggc gccaggtagg cgtcggctgc ggtcgtggag aagcgctcgt actccctgaa
     4261 ctccgggagc acctcgctgc tcaaagagac gtatacgtcg gggagcgcct ccctgagcgc
     4321 ctcgccgacc cgctcctcgt gctcggggtg cgcgaacgcg aagaggaggc acaccgcgac
     4381 ggcctcgacc tcagcttctt cgacggcgga gacggcggcc atcaggcttt cgtcgtcgag
     4441 cggtaagatc tcgccctcag ggcccatcct ctcggttacg gtgaagcgca gctcgcgcgg
     4501 gacgaggggg ggagggcggt cggcggcgag gtcgtagagg gaggcgcggt tctggcgcgc
     4561 gatctcgagc acgtccctga aaccctccgt cgttacgagc gctatcctgg cgccgcggcg
     4621 ctcgaggaga gcgttagtgg ccaccgtcat cccgtgggct agggccgaga cggctccagg
     4681 ctccacctcc gaggtcctca cggcgttcat cacgccctcc gactggtcgc gcggcgtcga
     4741 tgggaccttc gccgtgacta gcgttccgtc cgagagggcg accaggtcgg tgaacgtgcc
     4801 gccgacgtcc accccgagcc gtgtctcgct caaggcgcta tccctgctcg gctacgagcc
     4861 ggcgttcctg cgggtcttca gcgtagatgt ccttgcggta ggtctcctgg gcgagggcgt
     4921 agcacacgac gctcagggcg cacagtgcgg cgacgtaagc cgctatcagg gtcgtcccgc
     4981 cctgcgggta gagccaggtt gctatgagcg gcgccagagc gcccccgaaa atgcccgaga
     5041 actggtagct gaaagaggct ccactgtagc gcacccgtgt gccgaagagc tcggcgtaga
     5101 agctgccctg gacggcgtag actgcggcgt gggcgatcga gagcccgacg atggtggcga
     5161 ggatgatcag gatcgtggac ttggtgttga gcagcccgaa gaacgggaac gtccagatcc
     5221 ccagcagcgc cgcccctcct atcaggacag gtttcctgcc cattctgtcc gagagcgagc
     5281 cgaaggaggg gatggtgaag atctcgatca gggctgcgat ggagaccgcc agcagcccga
     5341 tgttcgggga gagaccgagg ttcgcggaga tgtgggtcag cgagtagacg gcgagtatgt
     5401 agaaggcagc gtctatgccg atgcgcgagc ctacgcccag ggccacgttc ttcgggtacg
     5461 tcctgagcac gtcgattatg ggcatcctgg cctcggtccc cgactcccgc acctggctga
     5521 acgccgggct ctcgtagatt ctcagacgga tgaaaagccc gactcctatc aggattatgc
     5581 tcaggaggaa cggcaccctc cagccccaga cctcgaacgc gtcgcccgtg atggctgaga
     5641 cggcggcgaa gacgcccgtc gcgagcacca gcccggcagg ggcccccatc tgcggccagc
     5701 taccgttgag cccccgctgt tccggtgggg agtgctcgac ggacatcagg accgcgccgc
     5761 cccactcgcc gccgagccca agaccctggg tgaggcgcag cacgacgagc aggatgggag
     5821 cccagactcc tatctgatcg aagctcggca ggcacccgac caggaaggtc gctacgccca
     5881 tgatcaggag cgttgtaacc agcatggcct tgcgcccgat cctgtccccg aagtgaccga
     5941 agatgatgcc gcccagaggc ctcgccgcga agccgacggc gaaggtggag aacgacaaca
     6001 gggttcccgc cagctcgctg aagtccggga agaacagtgc cgggaagacc agggcggccg
     6061 ccgtgccgta gatgaagtag tcgtaccact cgatcgtggt gccgataaag ctcgaaacga
     6121 cgaccttccg gatcgagctc ggaacccccg tgtcctcgcc gcctaccctc tgcataattc
     6181 ttactcccct ctcgctcccc gcatcgaacg gtcctcaccc gctattctgt caaacctcgt
     6241 catctg
//
LOCUS       DASTUV010000072         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_920923, whole genome shotgun sequence.
ACCESSION   DASTUV010000072 DASTUV010000000
VERSION     DASTUV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920923"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFT03_01615"
     CDS             <1..267
                     /locus_tag="VFT03_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU4846867.1"
                     /translation="GTLSPGLLAALGLDAGRMSPGFLTMLGVSALAGLIATTAAALAA
                     YYGSILSYRAGLDPDTYGIPIITSAVDLLGFMSLIIALVVFQLA"
     gene            279..1478
                     /locus_tag="VFT03_01620"
     CDS             279..1478
                     /locus_tag="VFT03_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEU4846868.1"
                     /translation="MRDSSPRNLKDMLAEAKDTSELMVDLAYAAIFYNSEDLSEEVFR
                     LEERLNDLVFDMRSLAIFAARSHSDAEQMAGILQVVQDIEKIGNAAYDIAKIVVKRLG
                     IPPELLHDIPEAEEIPSRVRIHVESYLDGRSLGDVDLPVETGMRPIAIRREDEWDFEV
                     EDEDVLRSGDVLFVQGPPEGVAQIRELAGARPMEAAPEPTQDGSRLSELERAVDVLIE
                     MKNLSEVSVGLAYSALLYNDAGLAREVVAIEDEMDEMRYRLERWVLLAACHVEDPSRL
                     RGMLHLAIAAEAISDGAQEMVWMVEKGEEIHPVLSAAVEESDEVVIKLTVAPGSTADG
                     KTLGELRLETETGMFVLAVNRSGRWSYRPRDTYTLRAGDSLLVTGAPEGLEPLAELFG
                     LELEEPE"
     gene            1721..>2306
                     /locus_tag="VFT03_01625"
     CDS             1721..>2306
                     /locus_tag="VFT03_01625"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEU4846869.1"
                     /translation="MPPALFSTDKAGYAALFSSEMALVMVVTLVLTALAARRIGGPYA
                     WIPASATCAAGAMMLYPVAVTRYDAVVALSLAVAAFSFGGRYLVAYASLGFGAAAKLV
                     PALATLPLAVFRRDAARGYAAFFGVLALFFVPALILGGGDFAGSFAYHADRSLQIESL
                     AASVLIQLGRVSSVVFEYGAFEAQGRGVELASSLS"
BASE COUNT          376 a          807 c          727 g          396 t
ORIGIN      
        1 ggcaccctga gccccggcct cctcgcggcg ctggggctgg atgcgggccg gatgagcccg
       61 ggcttcctga cgatgctcgg cgtcagcgcg ctcgctggcc tcatcgcgac gaccgctgcc
      121 gcgctcgcgg cctactacgg ctcgatcctc agctacaggg ccggcctcga ccccgacacc
      181 tacggcatac cgatcatcac ctccgcggtg gacctgctcg gcttcatgag ccttataata
      241 gccctcgtgg tatttcaact ggcataggtt aggaaagcat gcgcgactct tctccgagaa
      301 acctcaagga catgctggcc gaggcgaaag acacctcgga gctgatggtt gacctggcct
      361 acgccgcgat cttctacaac tccgaagacc tctccgagga ggtcttcagg ctcgaggaac
      421 gcctcaatga cctcgtcttc gatatgcgga gcctcgcgat cttcgcagcc aggagccact
      481 ccgacgccga gcagatggcc ggtatcctgc aggtcgtcca ggacatagag aagatcggca
      541 acgccgccta cgacatagcc aagatcgtgg tcaagaggct cggaataccg cccgagctcc
      601 tgcacgacat ccctgaggcg gaggagatcc cctcccgcgt ccgcatccac gtggagagct
      661 acctggatgg acgctctctg ggagatgtgg atcttccggt cgagacgggg atgcgcccca
      721 tagccatccg cagggaagac gagtgggact tcgaggtcga ggacgaggac gtgctgcgct
      781 cgggagacgt gctcttcgta cagggaccgc cggagggtgt ggcgcagata agggagctcg
      841 ccggcgcccg cccgatggag gcggctcccg agcccaccca ggacggaagc aggctctccg
      901 agctggagcg ggctgtggac gtcctgatcg agatgaagaa cctctccgag gtctccgtgg
      961 ggctcgccta ctccgccctc ctctacaacg acgcggggct cgcccgcgag gtcgtcgcca
     1021 tagaggacga gatggacgag atgcgctacc gcctcgaacg ctgggtcctg ctcgccgcct
     1081 gccacgtcga agatccctcg cgcctgaggg gcatgctcca cctcgccatc gccgccgagg
     1141 ccatcagcga cggcgcccag gagatggtat ggatggtgga gaaaggagag gagatccacc
     1201 ccgtcctctc cgccgccgtc gaagagtccg acgaggtcgt catcaagctg accgtcgctc
     1261 ccggctccac cgcggacggc aagacccttg gggagctgcg gctcgagacc gagacgggca
     1321 tgttcgtcct cgccgtcaac cggagcgggc gctggagcta ccggccccgc gacacctaca
     1381 cactgagggc cggggactcg ctgctcgtca ctggcgcccc agagggcctc gaacccctcg
     1441 ccgagctctt cggcctggag ctggaggaac cggaataacg gggaacactc cccacaaggc
     1501 ttccctccgg tcggtccttc ttttctgggc gccgctcgtt ctgctgggga ctctccttta
     1561 ccggcaactg gtcaccctgt ccgcaggact gccgccggac ctcagggaga actcgaacga
     1621 cctcgagatc taccgccaga ccggggaagc catcctgcgc ggagcactcc cttaccgcga
     1681 cttctttctc gagtacccgc ccggcagcct cccagccttc atcccacctg cgctcttcag
     1741 cacggacaag gctggctacg ccgccctgtt ctcctcggag atggcgctcg ttatggtggt
     1801 gacgctcgtc ctcacagcgc tcgctgcacg cagaatcggg ggaccctacg cctggatccc
     1861 cgcgtcggcg acctgcgccg ccggcgcgat gatgctctat ccggtcgccg tgacccgcta
     1921 cgacgccgtg gtagccctct cgctcgccgt cgccgccttc tcgttcggcg gacgatacct
     1981 cgtcgcctac gcctcgctcg gcttcggggc ggccgcgaag ctggtcccag cgctggcgac
     2041 cctgcccctg gctgtcttcc ggcgcgacgc ggcgcgcggc tacgcagcct tcttcggggt
     2101 cctggcgctc ttcttcgtgc ccgcattgat cctcggaggg ggcgacttcg cgggcagctt
     2161 cgcgtaccac gccgaccgca gcctgcagat agagagcctc gcggcttctg tcttgataca
     2221 actcggccgg gtgagtagcg tcgtcttcga gtacggcgcc ttcgaggcgc agggccgggg
     2281 cgtcgagctc gcgagctcat tgagct
//
LOCUS       DASTUV010000073         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_599304, whole genome shotgun sequence.
ACCESSION   DASTUV010000073 DASTUV010000000
VERSION     DASTUV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599304"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFT03_01630"
     CDS             <1..441
                     /locus_tag="VFT03_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU4846870.1"
                     /translation="AFSAVLGQVLSFLNVPQELAQLVADLQVNRWVVFVIMNLLFLFL
                     GCFIPPVAIILVTMPVLFPIITELGFDPIWFGVVMTLNMEMGLITPPVGLNLYVVQGI
                     APDIPLRDILAGSLPYVAVLALGIIILAFSPGLVTWLPNQTMGG"
     gene            451..1083
                     /locus_tag="VFT03_01635"
     CDS             451..1083
                     /locus_tag="VFT03_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU4846871.1"
                     /translation="MSSRELRIWGRSNSINVQKVLWCCGELDVPYERIDLGGIFGGNR
                     EPEYLRLNPNGLVPTISDGDFVLWESNAIVRYLAARHGMGTLCPEDLARRADADRWMD
                     WQLGTLWANLRPAFIGLVRTPPQNRDRASIEAAISKTTENLAILDSHLAGREYVAGSA
                     FTIADIPLGATAYRWFSLDFGRPPMRNLEAWYERLCSRAPYRANVMLPLS"
     gene            1291..2751
                     /locus_tag="VFT03_01640"
     CDS             1291..2751
                     /locus_tag="VFT03_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU4846872.1"
                     /translation="MTTGVEPTDHERGLRRELRFWEAIALSIGIMAPGAAMALNGTLP
                     ASLVGRAVPLAFVFATVGIIFVSYAFIRLTGYFNHAGSAYALSGVTLGPRAGFFSGWA
                     LLGTYLAFTAASTAEVGLFGQAFFDGTGIWPNAEWLVISLVAAALIWLIAYGDIRVAT
                     RSLLTMEGITVALIVVVVVVIFARVFAGTAPSGQSFTLSPFTPPSGVGLGTVALAAVF
                     GLLSFGGFEGAASLGEETDNPRRNIPRAIATAVLTMGVFYIIVMLAQTLGFGIDKQGT
                     ESFAGSSSPLGDLSRSYVGPIMSDAINFGVMVSAFASALGTATAGSRMLFALCRDGFL
                     SRRLGETSDRTGAPANALVVVMVIAIVAFVAQRIGGVSAVNAFFYPGTIGVLSMLIAY
                     IVTNVGALRFLFLSGRVRAWEAVFPVIALVILVYVIYANVYPVPDFPFNIFPYVVAAW
                     LVLGLGIVLLVPGLARRIGVNLAEREGLALEERPGS"
BASE COUNT          442 a          892 c          889 g          597 t
ORIGIN      
        1 gccttctccg cggtgctcgg ccaggtgttg agcttcctca acgttcccca ggagctggcg
       61 caactcgtag ccgatcttca ggtgaacagg tgggtggtgt tcgtgatcat gaacctgctg
      121 ttcctgtttt tggggtgctt cattccgccc gtggcgatta tcctcgttac catgccggtc
      181 ctgttcccga tcatcacgga gctcgggttc gatcctatat ggttcggcgt ggtgatgacc
      241 ctgaacatgg agatgggcct gatcacaccg cctgtaggcc tgaacctgta cgtagtgcag
      301 ggaatcgcgc ccgacatccc gctgcgcgac atactcgcgg gctcgctccc ctacgtggcc
      361 gtcctggcgc tagggataat catcctcgct ttctcccctg gcctggtaac gtggctgccg
      421 aaccagacga tgggaggatg agcgcggcgc atgtcgtcgc gtgagctccg gatctggggt
      481 agaagcaact ccataaacgt ccagaaagtg ctgtggtgct gcggagagct ggatgttccc
      541 tatgagcgca tcgatctcgg cggtattttc ggcggcaaca gggagccaga gtaccttcgg
      601 ttgaacccca acggtctggt gccgacgatc tctgatggcg atttcgtgct ctgggagtcc
      661 aacgccatcg tgcggtacct cgccgccaga cacgggatgg gcacgttgtg cccggaggac
      721 ctcgcacggc gggccgacgc tgacaggtgg atggactggc agttgggtac gctctgggcc
      781 aatctccgcc cggcgtttat cggacttgtc cgcacgccgc cgcaaaatag agatcgagct
      841 tctatagagg cggctataag caagacgacc gaaaacctgg ctatactcga ctcgcacctg
      901 gctggtcgag aatacgttgc cggatcggcg tttacaattg cagacatacc tctcggggcg
      961 acggcatacc gctggtttag cctggacttc ggacgacctc ccatgcgcaa cctggaagcc
     1021 tggtacgaac gcctgtgttc tcgagctcct tacagagcga acgtcatgtt gccgctttcc
     1081 tgaaagacga tgcgccaaag acggtgggaa gctccagcac gcccgcggcc gatgttctct
     1141 gctaccacct cccgctatgg cattattctc atggtggaat gggttgaccc gctatggcgg
     1201 ggggaaggag gagcgcaaga tgccgcaact cgtagctatt gaccggcgcg gacccggtcc
     1261 ggacggaggc gtcatctccg gaggaatccg atgacgaccg gggttgagcc gaccgaccac
     1321 gagcggggtc tgcggcgcga gctgcgcttc tgggaggcga tcgcgctgtc tatcggcatc
     1381 atggccccgg gcgccgcgat ggccctgaac ggcacgctgc cggcaagcct cgttgggagg
     1441 gcggtcccgc tggcgttcgt cttcgccacc gtgggcatta ttttcgtctc ctacgccttc
     1501 atccggctca cgggctactt caaccacgcg ggctccgcct acgccctctc cggcgtaacc
     1561 cttgggcccc gagcaggctt cttctccggc tgggccctcc ttggcacgta tttggccttc
     1621 acagccgcct ccaccgccga ggtggggctc ttcgggcagg ccttcttcga cgggacgggt
     1681 atctggccga acgccgagtg gctcgtgatc tcgctggtgg ctgccgcgct gatctggtta
     1741 atagcgtacg gggacatccg ggtggcaacc cgctcgttgc tcactatgga ggggatcacc
     1801 gtcgcgctga tcgtggtcgt ggtcgtggtc atctttgcca gggtgttcgc cggcacggct
     1861 ccgagcggtc agagcttcac gctctccccc ttcacgccgc cgagcggcgt gggcctcggc
     1921 acggtagctc tagccgcggt cttcgggctc ctctcgttcg gaggtttcga gggagcggcc
     1981 tctctaggcg aggagacgga caacccccgc cgcaacatcc cccgcgccat cgcgaccgcc
     2041 gtgctgacca tgggcgtctt ctacattatc gtcatgctgg cacagaccct gggcttcggg
     2101 atcgataaac agggtacgga gagcttcgcg ggctcgtcct cgcctctagg tgacctctca
     2161 cgtagctacg tcgggcccat catgtccgac gccataaact tcggtgtgat ggtcagtgcc
     2221 ttcgccagcg ccctcggcac cgctaccgcg ggctcgcgca tgctcttcgc tctctgtcgt
     2281 gacggcttcc tctcgcgccg cctgggcgag acgtcggatc gcaccggagc ccccgccaac
     2341 gccctggtag tcgtcatggt gatcgcgata gttgccttcg tggctcagcg cataggcggc
     2401 gtctccgcgg tgaacgcttt cttctatccg ggcactatag gagtgctgag catgctgata
     2461 gcctacatcg tcacgaacgt gggcgccctc aggttcttgt tcctgagcgg cagggtgcgc
     2521 gcctgggagg ccgtcttccc cgttatcgcg ctcgtgatcc tggtctacgt gatctacgca
     2581 aacgtctacc cggtgccgga cttcccgttc aacatcttcc cctacgtcgt agcggcttgg
     2641 ctggtgctgg ggctggggat tgtgctgctc gtgcccggac tggctcggcg tatcggcgta
     2701 aacctggccg agcgggaggg actggcgctc gaggagagac ccggctcata accctatgac
     2761 caggagccgg catcgcccgc atcggctttc gtcagtccct gagggccctt gacgcagagc
//
LOCUS       DASTUV010000074         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_134560, whole genome shotgun sequence.
ACCESSION   DASTUV010000074 DASTUV010000000
VERSION     DASTUV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134560"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..281
                     /locus_tag="VFT03_01645"
     CDS             15..281
                     /locus_tag="VFT03_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846873.1"
                     /translation="MSSAEGNVIIRAYSPGRYPILVVELKGGGLRTVYYETGYDLGRA
                     KEVEEDWLEENAIGRHSFIEVSPPREMPAASLEGYVNWEVLEGT"
     gene            complement(312..2357)
                     /gene="pknB"
                     /locus_tag="VFT03_01650"
     CDS             complement(312..2357)
                     /gene="pknB"
                     /locus_tag="VFT03_01650"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU4846874.1"
                     /translation="MQQLVDNRYSLVRPLGSGGMADVFLAHDSILDRDVALKVMSTRY
                     ASDDEFVERFKREAQSAAALTHPNIVSIFDRGASEDGTYYIAMEYLSGGTLKDRIMRK
                     GALPARTAAAVALQIAEALQAAHERGVIHRDIKPHNILITESGDVKVTDFGIARAASS
                     STMTRTGSILGTAHYISPEQAMGEHVGPSSDLYSLGVVLYEMLTGELPYDADTPLGIA
                     MKHVNGHLRQPKEVNPSIPASINAITCRLLAKDPEHRYASDAELIEDLERVAAGLEPA
                     SATTEMIAARPMPAATAPTRVAPPPPAAGRNGKRRRPWPFVLILLGLLLLAPIAWAAY
                     DFLYGQQEEPVARIAVPDLVGMTRQEAEDRVGKDFEISVENEVQDDKPVDTIVSQDPE
                     SGGKAEKGSTISVTVVGTQMAKVPNVVGQSRDAAQAALERVGFQVKVEEAESSFDDEG
                     AVTGQDPRSGSSVEKGSQVTITVGTGPSTVKVPSVYGNNPDQAAGILADYDLKLGTVS
                     KDYTSEVAEGVIFYQDPAQGQSVEPGSSVAVIVSLGVEQVEVPEVYGLTLTQAKNAVV
                     NAGFKYGVVAVEPNNDEAAGTALSTDPASGTLLAPGATVTIYYSSGPTQPTSASPAAD
                     EGNNGEGNNGANIRETLRDAFQGNRVVNQGNNRNNGGDGGGNGNGNRNGKGNGGGNN"
     gene            complement(2376..>2736)
                     /locus_tag="VFT03_01655"
     CDS             complement(2376..>2736)
                     /locus_tag="VFT03_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU4846875.1"
                     /translation="RSPDGIILDRPAPSVRRRVLDAGTAQTLNGMMQGVITDGGLTEA
                     EVPGVKVAGKTGTAEAPPRQPHSWFVAFAPADDPEIAVAVMVENGGEIGPQGDAATPA
                     IPIAQDLMEAYLKGEGG"
BASE COUNT          389 a          901 c          904 g          542 t
ORIGIN      
        1 tataaactca gggcatgtca tcggcggagg gaaacgtgat tatccgggca tactctcccg
       61 gcagataccc catcctcgtc gtcgagctca aaggcggcgg gctgcgcacc gtctactacg
      121 agaccggcta cgacctcggg agagcgaaag aggtcgaaga ggactggctc gaagagaacg
      181 ccataggccg ccacagcttc atcgaggtga gcccgccacg ggagatgccc gccgcctcgc
      241 tagagggcta cgtcaactgg gaggttctcg aggggacctg agaggaatgg atcccgggcc
      301 agagggcctg cctagttgtt gcccccgccg ttaccttttc cattgcggtt tccattgccg
      361 tttccgccac cgtcaccgcc gttgttgcga ttgttcccct ggtttaccac cctgtttccc
      421 tggaaggcat ccctgagcgt ttcccggatg ttcgcgccgt tgttgccctc gccgttgttg
      481 ccctcgtcgg ctgccgggga ggccgaggta ggttgggtag gaccgctgct gtagtagatg
      541 gtcaccgtgg cgccaggtgc gagcagcgtc ccggatgccg ggtccgtcga tagagctgtg
      601 ccggcggcct cgtcattgtt gggctcgact gcgactacgc catatttgaa gccggcgtta
      661 accacggcgt ttttggcttg cgtaagggtg aggccgtaga cctcggggac ctctacctgc
      721 tctacgccga ggctcacaat gacggccacc gagctgccgg gctcgacgct ctgtccctgg
      781 gccgggtcct ggtagaagat cacgccctcc gctacctcgc tggtgtagtc cttgctcacc
      841 gtgccgagct tcagatcgta gtcagcgagt ataccggccg cctggtccgg gttgttgccg
      901 tagacgctcg ggaccttcac cgtagagggt cctgtcccga cggtgatcgt cacctgcgag
      961 cctttctcga ccgacgaccc gctccgcggg tcctggcctg tcacggcgcc ctcatcatcg
     1021 aacgagctct ccgcctcctc gaccttgacc tggaagccga ccctctcgag ggccgcctgc
     1081 gccgcgtccc ggctctggcc gacgacgttc ggcacctttg ccatctgcgt ccctacgacg
     1141 gtgaccgaga tggtcgagcc cttctcggcc ttgccgccgc tctcggggtc ctggcttacg
     1201 atggtgtcca caggcttgtc gtcctggacc tcgttctcga cggagatctc gaagtctttt
     1261 ccgaccctgt cctcggcttc ttgcctggtc atgccgacca ggtcggggac ggcgatcctg
     1321 gcgaccggtt cctcctgctg cccgtacagg aagtcgtatg cggcccaggc gatcggggca
     1381 aggagcagca gccccaggag gatcaggaca aaaggccacg gtcgccgacg cttgccgttc
     1441 ctgcccgctg ccgggggcgg cggggccacg cgggtgggcg cggtcgccgc cggcatggga
     1501 cgggccgcga tcatctctgt cgtcgcgctc gcaggctcca ggcccgcggc tacgcgctcg
     1561 aggtcctcta tgagctcggc gtcgctagcg tagcggtgct cggggtcctt ggccagcagc
     1621 cggcacgtta tggcgttgat gctggccggg atcgaggggt tgacctcctt cggctgcctg
     1681 aggtgcccgt tgacgtgctt catcgcgatc cccagaggcg tatccgcgtc gtaggggagc
     1741 tcgccggtta gcatctcgta aaggaccacg cccagagagt agaggtcgct cgaaggcccc
     1801 acgtgctcgc ccatcgcctg ctcgggcgag atgtagtggg ctgttccgag gattgagccg
     1861 gtacgggtca tggtggagga tgaggcggcc ctggcgatgc cgaagtccgt aaccttcacg
     1921 tcccccgact cggtgatgag tatgttgtgc ggctttatgt cgcggtggat cacgccccgc
     1981 tcgtgggcgg cctgcagcgc ctcggcgatc tggagcgcca ccgcagccgc cgtgcgggcc
     2041 gggagcgcgc ccttgcgcat gatcctgtcc ttcagggtgc cacccgacag gtactccatg
     2101 gctatgtagt acgtcccgtc ctccgaggcg ccccggtcga aaatagagac gatgttcggg
     2161 tgggtgagcg cggccgcgct ctgcgcctcg cgcttgaatc gctcgacgaa ctcgtcgtcg
     2221 ctggcgtagc gggtgctcat caccttcagg gcgacgtccc tgtcgaggat gctgtcgtgg
     2281 gccaggaaca cgtcggccat ccccccgctg ccaaggggcc tgaccaggct gtagcgattg
     2341 tctactagct gctgcatgac cttggcatta tagcctcacc caccctcacc cttcaggtag
     2401 gcctccatta ggtcctgggc tatgggaatg gcaggcgtgg ccgcgtcgcc ctgcggcccg
     2461 atctcgccgc cgttctcgac catgacggcg acggctatct cggggtcgtc agcgggggcg
     2521 aaggcgacga accaggagtg cggctggcgc gggggcgcct cggcggttcc cgtcttgcca
     2581 gccaccttca ctccggggac ctcggcctcg gtcagcccgc cgtccgtgat cacgccctgc
     2641 atcatcccgt ttagcgtctg ggcggtcccc gcgtcgagca cccgcctgcg gacgctgggg
     2701 gcaggtctgt cgaggatgat gccgtcaggg gagcgt
//
LOCUS       DASTUV010000075         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1635977, whole genome shotgun sequence.
ACCESSION   DASTUV010000075 DASTUV010000000
VERSION     DASTUV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635977"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFT03_01660"
     CDS             complement(<1..215)
                     /locus_tag="VFT03_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846876.1"
                     /translation="MSGPGHGSVGTEAEAVRIWLLGGFKVSIGSRLIEKRAWRLRKAS
                     ALVKLLALTPGNRLHREQVMDLLWPDLG"
     gene            430..3177
                     /locus_tag="VFT03_01665"
     CDS             430..3177
                     /locus_tag="VFT03_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEU4846877.1"
                     /translation="MKKRKNADPSHDVLGYERRPLDAIFDPDTVAVVGASDRPGSVGR
                     TIMWNLVSNPFGGTVYPVNSKRQNVLGIKAYPSVSEVPELVDLAVIASPAPTVPGIIR
                     ECVECGVRGAIIISAGFRETGPEGAELERQVLEEARRGRMRIIGPNCLGVMNPTTGLN
                     ATFAGAMARPGNVGFLSQSGALMTAILDRSFVENVGFSAFVSVGSMMDVGWGDLIYYL
                     GDDRKTESIVIYMESVGDARSFFSAAREVALTKPIIVIKAGRTAAASHAAASHTGSLT
                     GSDEVLDAAFMRSGVLRVDNISDLFDMAETLGKQPRPKGPRLTILTNAGGPGVLATDA
                     LIARGGELAGISPETMQELNRFLPAPWSHGNPIDVLGDADPVRYANALETASKDPGSD
                     GLLVILTPQDMTEPTETAQGLVPYAHSTGKPILASWMGGVEVAAGTSILNGAGIPTFD
                     YPDDAARSFTNKWRYTYNLRGIYETPELADDADVDRERAGELLASARDAGRTILTEFE
                     SKAFLEAYGIPTVETRVATTADEAVELAEHFGYPVVLKLNSQTITHKTDVGGVRLNLT
                     GAEEVRLAFEEMERSIAENFSPEDFAGVTVQPMVSLEGYELIMGSSLDAQFGPVLLFG
                     SGGQLVEVYRDRALALPPLTTTLARRTMERTRIFEALQGVRGRPPVDTTSLEKLFVRF
                     SQLVVEQPWIKEMDINPLLASPERLLALDARVVLHPPDTGEEDLPRPAIRPYPRQFMW
                     VEETRDGEPITIRPIRPEDEPLMVRFHESLSEESVYMRYFHMLGLSQRTAHERLTRIC
                     FIDYDREMALVAERTDPETREREIMGIARLSRLATGFDDEAELSVLVSDRYQRRGIGT
                     LLVSKLLEVARDEGIQHITAEILFENYPMQRIFKKLGFRLHRDTEEMVVKADLDLYQP
                     A"
BASE COUNT          675 a         1185 c         1065 g          534 t
ORIGIN      
        1 ccaaggtccg gccagagcaa gtccatgacc tgctcgcgat ggagacgatt gccaggggtg
       61 agagcaagca gcttcaccag ggcagaagcc ttcctcaggc gccaggcgcg cttctcgatt
      121 agccttgaac ctatggaaac cttgaagcca ccgagcagcc agatgcgtac ggcctcagcc
      181 tccgtaccca cggagccatg cccgggtccg ctcatgaaat caatctagca taaccggaag
      241 cgtggaggga agtcgctctg gcctgccgcg ctggtcggca cttcagcgaa agtcgagcgt
      301 ggaaggaatt acgtcccggc tcggccgtct gtgctacgaa ctagaatacc gggaacttag
      361 ttttcgtccc gcgatgggta cttacgaagc tgagcggaga cccggctgac cggctacaag
      421 gagcggcgga tgaagaagcg caagaacgcg gacccgtccc acgacgtcct cggctacgag
      481 cgcaggccgc tcgacgctat cttcgatccg gacaccgtcg ccgtcgtcgg ggcgagcgac
      541 cggccgggga gcgtcgggcg tacgatcatg tggaaccttg tcagtaaccc cttcgggggg
      601 acggtttatc ctgtgaactc caagcgccag aacgtcctcg ggataaaggc ctacccgagc
      661 gtctccgagg taccagagct cgtggacctg gcggtcatcg cctctcccgc gccgacggtg
      721 ccagggatca tccgcgagtg cgtcgagtgt ggggtgaggg gtgcgatcat catctccgcg
      781 ggcttcaggg agacgggccc cgaaggcgcc gagctggaga ggcaggtcct cgaagaggcc
      841 aggcggggac gcatgcggat catcggtccc aactgcctcg gggtgatgaa cccgaccacg
      901 ggtctcaacg ccaccttcgc cggagctatg gcgcgtcccg gcaacgtcgg cttcctgagc
      961 cagagcgggg cgctcatgac ggccatcctg gaccgcagct tcgtggagaa cgtcggcttc
     1021 tcggctttcg tcagcgtcgg ctccatgatg gacgtggggt ggggggacct gatctactac
     1081 ctcggcgacg acaggaagac agagagcatc gtgatctaca tggagtcggt gggcgacgcc
     1141 cgctccttct tctcggccgc ccgcgaggtg gccctcacca aaccgataat cgtcatcaag
     1201 gccggccgca ccgccgcagc ctcccacgcc gcagcctccc acaccggttc cctgaccggc
     1261 tccgacgagg tcctcgacgc cgcgttcatg cgcagcggcg tcctgcgcgt ggacaacatc
     1321 tcggacctct tcgacatggc cgagaccctg ggtaaacagc cgcgcccgaa ggggccgcgc
     1381 cttacgatcc tgaccaacgc tggcggcccc ggcgtgctgg cgacagacgc tctcatagcc
     1441 cgcggcggcg agctcgccgg gatctccccc gagaccatgc aggagctgaa ccgcttcctc
     1501 cccgcgccct ggagccacgg caatcccata gacgtcctcg gcgacgccga ccccgtacgc
     1561 tacgcaaatg ccctggagac cgcgtcgaag gacccgggga gcgacggcct gctcgtgatc
     1621 ctcacccccc aggacatgac ggaaccgacc gagacagccc agggcctcgt tccctacgcg
     1681 cacagcaccg gcaagccgat cctggccagc tggatgggcg gcgtcgaggt cgccgccggc
     1741 acgtcgatcc tgaacggcgc cggtataccg accttcgact accctgacga cgcggcccgc
     1801 tccttcacca acaagtggcg ctacacctac aacctgcgcg gcatctacga gacgcccgag
     1861 ctggccgacg acgccgacgt ggaccgcgag cgggccggag agctcctcgc aagcgcccgc
     1921 gatgcggggc gcaccatcct cacggagttc gagtccaaag ctttcctcga ggcctacggc
     1981 atcccgaccg tcgagacgcg ggtggcgacg acagccgacg aggccgtcga gctcgccgaa
     2041 cacttcggat atcccgtcgt actcaagctg aactcgcaga ccataaccca caagacggac
     2101 gtcggcggcg tgcgcctgaa cctcacagga gccgaagagg tacgcctggc cttcgaggag
     2161 atggagcgct cgatcgcgga gaacttctcg cccgaggact tcgcgggcgt caccgtgcaa
     2221 ccgatggtaa gcctggaagg ctacgagctg atcatgggga gcagcctgga cgcgcagttc
     2281 gggcccgtgc tgctcttcgg ctcgggcgga cagctcgtcg aggtctacag ggaccgcgcg
     2341 ctggccctcc cgccactcac caccacgctc gcccgccgca ccatggaacg cacccgcatc
     2401 ttcgaagcct tacagggcgt gcgcggccgc ccgccggtag acaccacgtc gctcgagaag
     2461 ctcttcgtgc gcttctccca gctcgtcgtg gagcagccgt ggataaagga gatggacatc
     2521 aacccgctgc tcgcctcccc ggagcgcctc ctcgctctcg acgcccgcgt ggtattgcac
     2581 ccgccggaca ccggcgaaga agatctgccc cgcccggcca tcagacccta cccgaggcag
     2641 ttcatgtggg tggaggagac gcgcgacggc gagcccataa cgatacgccc gatacggccc
     2701 gaagacgagc ccctgatggt caggttccac gagtcgctct cggaagagag cgtctacatg
     2761 cgctacttcc acatgcttgg cctgagccag cgcacggccc acgagcgcct tacgcgcatc
     2821 tgcttcatag actacgaccg cgagatggcc ctcgtcgccg agcgcacgga ccccgagaca
     2881 agggagcgag agattatggg catagcccgc ctgagccgcc tcgccaccgg cttcgacgac
     2941 gaggctgagc tatcggtcct cgtgagcgac cgctaccagc gccggggcat cggcaccctg
     3001 ctcgtgagca aactgctcga ggtagcccgc gacgaaggta tccagcacat aactgccgag
     3061 atcctcttcg agaactaccc aatgcaacgc atcttcaaga agctgggctt ccgcctccac
     3121 cgcgacacag aggagatggt cgtgaaggcc gacctcgacc tgtaccagcc ggcataaaat
     3181 agagcccgcc ctccgaagag ggccggcttc ctaccacccg cgctaacggc gcgaaggtta
     3241 tccgcaacta ctaccaccag acgacgcacg ccaccgggaa tcgttcccga aacgcgcgcg
     3301 cccgcgccgc ctatcagacc tcataaaccc agctattcgc taacgattcc cattaatcag
     3361 gaacaataat tgttcttgat taatgggctt gtgcggctat acttcggagg tgagcaagac
     3421 ggcttttaag gggagggtgc agcgttgacg gcgttgatg
//
LOCUS       DASTUV010000076         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1706980, whole genome shotgun sequence.
ACCESSION   DASTUV010000076 DASTUV010000000
VERSION     DASTUV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706980"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFT03_01670"
     CDS             <1..1146
                     /locus_tag="VFT03_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU4846878.1"
                     /translation="GAMARAAEELFGERIAGGLIVTRYGHEAASEKLEIIFAGHPEPD
                     ENSVEAARRAEELARSLGEGDLLLALISGGASALLADPAPPIELADLKMLTGALLRSG
                     ANISEINAVRKHVSVLKGGGLVKLAAPAPTLALLLSDVVGDDPSAIASGLTAPDPTTL
                     DDARRVLERYGIEPPESVAEHLKNSPETPKPGNPVFERVVNLICGSGRHAAEAAVAKA
                     RELGYEPMLLSTTITGDARGIASVYAAVIREVLESGNPLPPPCAVVSGGEATVTVRGD
                     GVGGPNQEFALALAVELDGIGGWAALAADTDGNDGPTDAAGGLVDGATADAIRSGGID
                     PEEALANNDAYAALEAGGAPLVTGPTGTNVNDLRVALVSGEGQVEPR"
     gene            1274..2953
                     /locus_tag="VFT03_01675"
     CDS             1274..2953
                     /locus_tag="VFT03_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HEU4846879.1"
                     /translation="MYEQVYEPVAGSLALSALVAAIPIVVLFVLLAGVRIAAQWASLA
                     TLAVALVIAVLVYGMPVGLALDSTLLGAAFGLFPIVWIVINAIFIYNVIVDTGHFDTI
                     RDSLAGISNDRRIQVVLIAFVFGALLEATAGFGTPVAITGSLMAGLGFEPIYAAALAL
                     LANTAPVAFGGFGIPILTGATVAGLDTMELSQMVGRQTPFLALIIPGMLVTVMAGFRR
                     MLEVWPVVAVSGITFAGTQFLVSNLLGPELADLLAAIITVVAVVALLAVWRPGSEWHF
                     EHEPPAAQRESFDEPPLGRTLYAWSPFVIIIALFLLAQVPPIKEAIGTTQETVNFPSA
                     EVSETTGLPAVPWPGLNEQVEQRPPVVPEPTPYPATLSTNWLSAAGTLILIADIISLI
                     VLRVGPGRALRIYGRSLNQLKWAILTIASILGLAYLLNYSGITYTLGLAFAATGALFP
                     FFAAFIGWLGVALTGSDTSSNALFANLQKVTAQQIGVSPVLTVGANSSGGVLGKMISP
                     QNLAVGTSATGLQGREGDLLRLVFKWSIGLTVVMAILVTLQAYVLKFMIPG"
     gene            complement(2957..3253)
                     /locus_tag="VFT03_01680"
     CDS             complement(2957..3253)
                     /locus_tag="VFT03_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEU4846880.1"
                     /translation="MGRYHAVVDAVRMFSLLAGSMVIFLGILRAASESRGAGGRVRAA
                     RRISDHAALGLEFFIAATILNLILNPTWTAVAATAFTIAIRQLLTLSLTLARAG"
     gene            complement(3256..3660)
                     /locus_tag="VFT03_01685"
     CDS             complement(3256..3660)
                     /locus_tag="VFT03_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEU4846881.1"
                     /translation="MDCLPLVRGGFNITAVEEYLITAVDYLRLVIEAIGAAVVGFGSV
                     AAAIRLLLTLLGIRDDSNTEIRLFLGRYLALGLEFQIGADILSTAVAPTFEDVLLLGA
                     IVVIRTVLNYFLSKELERERRQVTHESDALRG"
     gene            3751..>4067
                     /locus_tag="VFT03_01690"
     CDS             3751..>4067
                     /locus_tag="VFT03_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU4846882.1"
                     /translation="MAQEGSNLVRLDDFEGEIDEHWQEARGRTVLDKNGDEAGTVEEL
                     YIWEGPSTVHLIKVSSEEGSFLIPVHAATSVDEDGVKLETSRDKITSSPGYDSDDVPD
                     DEAR"
BASE COUNT          722 a         1341 c         1341 g          663 t
ORIGIN      
        1 ggcgctatgg cccgggccgc cgaggagcta ttcggcgagc gcatcgcggg cgggcttatc
       61 gtcaccaggt acggccacga ggcggcttcg gagaagctgg agatcatatt cgccggccac
      121 cccgagccgg acgaaaacag cgtggaggcg gcacgaagag ccgaggagct cgcacgctcc
      181 ctgggagagg gagaccttct tctcgccctc atctccggcg gcgcctcggc gctcctggcc
      241 gaccccgcgc cgccaataga gctcgcggac ctgaagatgc tcaccggggc gctgctgaga
      301 agcggggcga acataagcga gatcaacgca gtgcgcaagc acgtctccgt gctcaaaggc
      361 ggcggcctgg tgaagctcgc cgcccccgcg cccaccctcg ctctcctcct ctccgacgtg
      421 gtgggcgatg acccttccgc gatagccagt ggcctcaccg cccccgaccc gacgaccctc
      481 gacgacgcgc gaagggtcct cgaacgctac ggcatagaac cgcccgagag cgtcgccgag
      541 cacctgaaga actcccctga gacccccaag cccggcaacc ccgtcttcga gagggtggtg
      601 aacctgatct gcggcagcgg acggcacgcg gccgaggccg cggtagcgaa ggcgcgggag
      661 ctcgggtacg agcccatgtt gctctccacc accatcacag gggatgcccg cggcatagcc
      721 tcggtgtacg cggcggtgat ccgggaggtc ctggagagcg gcaacccgct ccccccaccc
      781 tgtgccgtcg tctccggcgg cgaggcgaca gtaacggttc gcggggacgg cgtgggcggc
      841 ccgaaccagg agttcgccct cgccctggcc gttgagctcg acggcatcgg gggctgggcg
      901 gcgctcgcgg cggacaccga cggcaacgac ggccccacag acgccgccgg cgggctggta
      961 gacggcgcga cggcggacgc catccgaagc ggtgggatcg acccagaaga agcgctggca
     1021 aacaacgatg cgtacgcggc gctcgaggcc ggaggagcgc ccctcgtcac aggccctacc
     1081 ggcaccaacg tcaacgacct gcgcgtggcg ctcgtctcag gggagggaca agtagagccc
     1141 cggtaacatg acttgcgccg ggtaccaggg agtgccatgc tggcgattct taaacccact
     1201 agccggcaga acgtagttgg taccaggctg ggaggctttg ctgccggcaa cgtccatagg
     1261 gaggtagggt ctcgtgtacg agcaggtcta cgagccggtc gcgggtagcc tggcactctc
     1321 ggcgctggtg gccgccatcc ccatagtggt gctcttcgtg ttgctggcgg gggtcaggat
     1381 cgcagcgcag tgggcgtcgc tcgcgaccct tgcggtagcg ctcgtcatcg ccgttctggt
     1441 ctacgggatg ccggtagggc tggcgctcga ctcgacactt ctaggggcgg cgttcgggct
     1501 cttcccgata gtctggatcg tcataaacgc catcttcatc tacaacgtga tcgtggacac
     1561 aggccacttc gacacgataa gagactctct ggcgggcatc agcaacgacc gcaggataca
     1621 ggtagtcctc atagctttcg tcttcggggc gctcctggag gcgacggcag gcttcgggac
     1681 gccggtggcg ataaccggct cgctcatggc gggcctcggc ttcgagccca tctacgccgc
     1741 ggctctggcg ctcctggcta acacggcgcc agtcgccttc ggagggttcg gcattccgat
     1801 cctgacaggc gccaccgtgg ccggccttga caccatggag ctctcgcaaa tggtcgggcg
     1861 ccagacgccg tttctcgcac tgatcatccc cgggatgctg gtgacggtga tggccggttt
     1921 ccggcggatg ctcgaggtgt ggcccgtcgt cgcggtgagc ggcatcacgt tcgccgggac
     1981 ccagttcctg gtctcgaacc tgctcggccc ggagctcgcg gacctccttg ccgcgataat
     2041 caccgtggtg gctgtcgtcg ccctgctggc ggtctggcgc cctggcagcg agtggcactt
     2101 cgagcacgag ccgccggcgg cccagaggga gagcttcgac gagccgcctc tcgggcgcac
     2161 gctctacgcc tggtcgccgt tcgtcatcat aatcgcgctg ttcctcctcg cccaggtgcc
     2221 cccgatcaag gaagccatag gcactacaca agagacggtg aacttcccga gcgccgaggt
     2281 gagcgagact accgggttgc cggcagttcc ctggccgggg ctcaacgagc aggtcgagca
     2341 aaggccgcct gtggtgccgg agcccacccc ctaccccgcc accctctcga ccaactggct
     2401 ctcggctgcg ggaacgctaa tcctgatagc ggacatcatc tccctcatcg tactcagggt
     2461 cgggccggga agagctctac ggatctatgg ccggtcgctc aaccagctca agtgggccat
     2521 cctcacgata gcctcgatcc tcgggctcgc gtacctgctc aactactcgg gaataaccta
     2581 cactctaggt ctggcgttcg ccgcgacggg agccctcttc ccgtttttcg ccgcgttcat
     2641 tgggtggctc ggggtggccc tcaccggctc ggacacctcg tccaacgcgc tgttcgccaa
     2701 tttgcagaag gtcacggccc agcagatcgg ggtgagtccc gtcctgaccg tgggcgccaa
     2761 ctcctccggc ggcgtcctgg ggaagatgat cagcccgcaa aacctcgccg tcggcacgag
     2821 cgccacgggt ctccagggca gggagggcga cctgttgcgc ctggtcttca agtggtcgat
     2881 cgggctgacc gtcgtgatgg cgatcctggt aacgctccag gcctacgtgt tgaagttcat
     2941 gatccccggc tagcggctac cccgctcgcg ccagcgtcaa ggagagcgtc aggagctggc
     3001 ggatagcgat ggtgaaggcc gtggccgcca ccgccgtcca cgtggggttg agtatcaggt
     3061 tgaggatggt cgccgcgatg aagaactcga ggccgagagc cgcgtggtcg gatatccgcc
     3121 gggcggccct caccctgcct ccggcgcccc tgctctcgct ggcggcgcgc aggatgccaa
     3181 ggaagatcac catgctacct gcgagcagcg agaacatccg taccgcgtcc accacggcgt
     3241 ggtatcgacc cacttctagc ctcgcaaggc gtcgctctcg tgcgtcacct gccggcgctc
     3301 gcgctcgagc tccttcgaga ggaagtagtt gaggactgtc cggatcacta ctatggcacc
     3361 gaggaggagc acgtcttcga aggtcggggc gaccgcagtg ctcaggatgt cggccccgat
     3421 ctgaaactcg agcccaagcg cgaggtaacg tccgaggaaa agccggatct cggtgttcga
     3481 gtcatccctt atgccaagca gggtgagcag aagcctgatc gccgccgcca cggagccaaa
     3541 ccccactacg gccgcgccta ttgcctcgat caccaaccgc aggtagtcga ccgccgtgat
     3601 cagatactct tccactgccg ttatgttgaa gcctccccgg actagaggca aacagtccac
     3661 ggatggacat tctcgggagc taccagcgtt tagcctccgc cgcgtgcagg aataacggta
     3721 ggaagacggc tttccaaggg agacagggtt atggctcaag aaggatccaa cctcgtcagg
     3781 ctcgacgact tcgagggcga gatagacgag cactggcagg aagcgagggg ccgcacggta
     3841 ctcgacaaga acggggacga ggccggcacc gtcgaggagc tctacatctg ggagggtccc
     3901 tccaccgtgc acctcatcaa ggtgagcagc gaggagggta gcttcctgat cccggtccac
     3961 gcagcgacca gtgtggacga ggacggggtg aagctggaga cgtctagaga caagatcacg
     4021 agctccccag gatacgactc ggacgacgtg ccggacgacg aggcccg
//
LOCUS       DASTUV010000077         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_241037, whole genome shotgun sequence.
ACCESSION   DASTUV010000077 DASTUV010000000
VERSION     DASTUV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241037"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFT03_01695"
     CDS             <1..877
                     /locus_tag="VFT03_01695"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU4846883.1"
                     /translation="DPHGFRPLCIGEVEGGYAVSSETCGLDIIGARFLREVAPGEVVV
                     IDDSGLHSFKGESPRPALCVFEYVYFARPDSRFDGREVAGARGRMGELLAEEAPAEAD
                     VVIPVPDSGISAAVGYSKRSGIPYAEGLIKNRYVGRTFIQPTDGMRQLGLRLKLNPLP
                     SVIEGRRVVVIDDSIVRGNTSRKLVKLLFDAGAMEVHFRVSSPPITGPCYYGIDMDTR
                     DQLVGANYSVEEIREHIGATTLAYLSTEAMVAATGQPRGRLCRACFDGDYPIPGSSEK
                     FALENRTHYAGTRGG"
     gene            874..1890
                     /gene="purM"
                     /locus_tag="VFT03_01700"
     CDS             874..1890
                     /gene="purM"
                     /locus_tag="VFT03_01700"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU4846884.1"
                     /translation="MTGSSYEAAGVSIERGEQAVDLMRSAVEATHGPEVLGSAGGFAG
                     LYALGGYTEPVLASTIDGVGTKVLVAREMGRYGSLGADIVNHCANDVLATGASPLLFL
                     DYVASGRLDPEAVAGVVRGAAEACSRLDIALVGGETAEMPGLYAGEDLDVVGACVGAC
                     ERSEVVTGERVRAGDAILGLASSGLHTNGYTLARKVVEAAGLSYSEAPEGFDRSLGQV
                     YLEPHRAYAREVAGLRDAVEVRGMAHITGGGLPGNLPRALGGLGARLYPSSWEEPPVF
                     ALIRALGNVPEGEMRRVFNLGIGFCAVVPAGEAEKGLETLRGAGCAAWRIGEVVEGGG
                     VEFG"
     gene            1883..2458
                     /gene="purN"
                     /locus_tag="VFT03_01705"
     CDS             1883..2458
                     /gene="purN"
                     /locus_tag="VFT03_01705"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU4846885.1"
                     /translation="MAERLPEGSRFAVLASGSGTNLQALLDAYPRELVVVAGDKKAAF
                     AFERARRVGVPVEHVDPAGFMSREDYDRELADRVAAHDVSLVVGAGYMRVLSPAFLER
                     FPAILNVHPSLLPEFRGLHAVKRALEANVKETGVTVHFMVEEVDAGPIVAQERVPVLP
                     SDDEATLLKRLHPVEHRLLVRAVADYFWGRV"
     gene            2455..>2688
                     /gene="purH"
                     /locus_tag="VFT03_01710"
     CDS             2455..>2688
                     /gene="purH"
                     /locus_tag="VFT03_01710"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563984.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU4846886.1"
                     /translation="MKRRALISVSDKKGIAAFARRLSRMGFEIISTGGTARTLVENRV
                     PVIPVSEITGEPEILGGRVKTLHPMLYGGILANL"
BASE COUNT          430 a          880 c          950 g          428 t
ORIGIN      
        1 cgacccgcac ggcttcaggc cgctctgcat cggcgaggtc gagggcggct acgccgtctc
       61 gagcgagacg tgcggcctcg acatcatcgg cgctcgcttc ctgcgggagg ttgctccggg
      121 cgaggtcgtc gtcatagacg attcgggcct gcacagcttc aaaggcgaga gcccgcgccc
      181 cgctctgtgc gtcttcgagt acgtgtactt cgcccgtccc gactcgcgct tcgacgggcg
      241 ggaggtcgcg ggggcgcgag ggaggatggg tgagctgctc gccgaggaag cgccggctga
      301 agccgacgtc gtcataccgg tgccggactc ggggatctcg gctgccgtcg ggtactcgaa
      361 gcggagcggc ataccctacg ccgagggcct cataaagaac cgctacgtcg gccggacgtt
      421 tatccaaccg acggatggta tgcgccagct cggccttcgt ctcaagctca acccgctccc
      481 ctcggtgata gagggcaggc gcgtcgtcgt catagacgac tccatcgtgc gcgggaacac
      541 gagccgcaaa ctcgtgaaac ttctcttcga tgcaggggcg atggaggtcc acttcagggt
      601 ctcctcgccg ccgatcacgg gtccctgcta ctacggtata gacatggata ccagagatca
      661 gctcgtcggc gccaactact cggtagagga gatccgggag cacataggcg ccaccacgct
      721 cgcctacctc tcgacagagg cgatggtcgc cgccacgggc cagcccagag gtcgcctgtg
      781 ccgcgcctgc ttcgacggag actacccgat ccccggttcc tccgagaagt tcgccctgga
      841 gaaccgtacc cactacgccg gcacgcgggg cggatgacag ggagctccta cgaggccgcc
      901 ggcgtctcca tagagcgcgg ggagcaggcc gtggacctca tgcgctccgc cgtggaagcg
      961 acccacgggc ccgaggtcct cggaagcgcc ggcggcttcg ccgggctcta cgccctcggc
     1021 ggatacacgg agccggtcct cgcctcgacc atagacggcg ttggtaccaa ggtcctcgtc
     1081 gccagagaga tggggcggta cggctctcta ggcgcggaca tcgtgaacca ctgcgccaac
     1141 gacgtcctcg ccaccggcgc gagcccgttg ctcttcctcg actacgtcgc gagcggcagg
     1201 ctcgaccccg aggccgtcgc cggggtcgta cggggagcag ccgaagcgtg cagtcggtta
     1261 gacatcgccc tcgtcggcgg tgagacggcg gagatgccgg gcctctacgc tggggaagat
     1321 ctcgacgtcg tcggagcgtg cgtcggagcg tgcgagagga gtgaggtcgt taccggtgag
     1381 cgggtgcggg ccggcgacgc catcctcggc ctcgcctcca gcggcctcca caccaacggg
     1441 tacacgctcg cgcgcaaggt agtcgaggca gctggcctct cgtactccga ggcgccggag
     1501 ggcttcgacc gttcgctcgg gcaggtctac ctggagccgc accgcgccta cgcgcgcgag
     1561 gtcgccggcc tgcgcgatgc ggtcgaggtg cgcgggatgg cccacataac cggcggcggg
     1621 ctccccggga acctgccgcg agctctgggc gggctggggg cgcggcttta cccctcatcg
     1681 tgggaagagc cgccggtgtt cgccctgatc cgagccctcg gcaacgtccc tgagggcgag
     1741 atgcgccgcg tcttcaacct tggcataggc ttctgcgccg tcgtgcccgc aggggaagcc
     1801 gagaagggac tggagacgct ccgtggcgcg ggatgcgcgg catggcgcat cggcgaagta
     1861 gtcgagggag ggggcgtcga gtttggctga acgactaccc gaggggtctc gtttcgccgt
     1921 actcgcctcg ggctcgggga cgaacctgca ggcgctcctc gatgcctacc cgcgggagct
     1981 cgtggtggtc gcaggggaca agaaggccgc cttcgccttc gagagggcgc ggcgggtcgg
     2041 agtgcccgtc gagcacgtcg accccgcggg cttcatgagc cgcgaggact acgaccgcga
     2101 gctcgcggac agagtagccg cccacgacgt ctccctcgtc gtcggggccg gttacatgcg
     2161 ggtcctgtcg cctgcgttcc tcgaacggtt cccggcgatc ctgaacgtcc acccttcgct
     2221 cctgccggag ttccggggcc tgcacgcggt aaagagggcg ctcgaggcaa acgtgaagga
     2281 aacgggggtt acggtgcatt tcatggtcga ggaggttgac gcggggccca tcgtggctca
     2341 ggagagggtg ccggtactgc cgtcagacga cgaggcgacc ctgctgaaac gtctgcaccc
     2401 ggtcgagcac cggctgctcg tgcgggcggt cgctgactac ttctggggaa gagtatgaag
     2461 cggcgggctc tcatctccgt ttcggacaaa aaaggcatcg cggcgttcgc gcgcaggctc
     2521 agcaggatgg ggttcgagat catctcgacc ggcggaacgg cgaggacgct cgtcgagaac
     2581 cgcgttccgg tgatccccgt ctctgagatc acgggggagc cggagatcct aggcgggcga
     2641 gtgaagacgc tccaccccat gctctacggc ggcatcctcg cgaacctc
//
LOCUS       DASTUV010000078         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_884903, whole genome shotgun sequence.
ACCESSION   DASTUV010000078 DASTUV010000000
VERSION     DASTUV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884903"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFT03_01715"
     CDS             <1..367
                     /locus_tag="VFT03_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4846887.1"
                     /translation="GASGVLHKSTPIKDIVEAVQMLRAGEALLSPAEVVEMLRLVGRE
                     RQEELAARQAVERLTPREKEVLQALAEGLESKEIAEKLNVTVETERTHMVNILHKLGV
                     HSRLQALVFAARYGVVRIR"
     gene            424..783
                     /locus_tag="VFT03_01720"
     CDS             424..783
                     /locus_tag="VFT03_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846888.1"
                     /translation="MSACAGELQSRKALILLANEPRAYRESIAQVLGQLRPNVEVITA
                     EPEELEERVLRLAPEMVIFSEAPGLVRERVPVWVELYPEYGRRSVVGVGEERSTIEEI
                     QLSHLLCVVDHAERLAH"
     gene            850..1188
                     /locus_tag="VFT03_01725"
     CDS             850..1188
                     /locus_tag="VFT03_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846889.1"
                     /translation="MNGARVLVALELTSYREAIAAAFRLLRPNVEVFEAEDEDLEREV
                     VRIGPHLVVCSRLTAWVENRAPNWVELYPGYSSYSVVSVRGERSTIEEIQLSHLLSLI
                     DETVKNGCSG"
     gene            complement(1185..1874)
                     /locus_tag="VFT03_01730"
     CDS             complement(1185..1874)
                     /locus_tag="VFT03_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846890.1"
                     /translation="MAQREFRLPGSSYEELVNIIVAYGTRDEASRPGDVGKLDAVHQS
                     SVSRNNGFLTQIGILQGESKKLITRRGRALALALARKDEEEIRRNWRAIVATNEFLQN
                     VVAAVKLREGMLYPTVQAYVAHAAGQPRNKPVMTGAGAIVEILKTAGLLREEAGELVA
                     TFDGELDPVETIATNELSADTQDVEEPVVSAMVEVGAAVKIHLHVQCTADEIDDIAPR
                     LKTLLRELSDP"
     gene            2020..>2466
                     /locus_tag="VFT03_01735"
     CDS             2020..>2466
                     /locus_tag="VFT03_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU4846891.1"
                     /translation="MRRGRPRTLTRREFLALAGAGALGASLLGAGCDLTTRLPNLPGQ
                     PPAGDTNVVLVILDSLRKDHIGAYGNDWIQTPNLDALAKESLRFTTAYPESAPTICAR
                     RAIHTGLRTWPFRDWKPPKGEDIILQGWQPIPEGQTHLAEIMKQNGY"
BASE COUNT          440 a          804 c          786 g          436 t
ORIGIN      
        1 cggcgcctcg ggcgtgctgc acaagtccac cccgataaag gacatagttg aagcggtgca
       61 aatgcttagg gccggtgagg cgctgctctc tcctgccgag gtcgtcgaga tgcttcgcct
      121 agtcggccgc gagcgccagg aagagctcgc ggcgcggcag gctgtggaga ggctgacacc
      181 tcgtgagaag gaagtgctcc aggccctcgc cgaaggcctc gagagcaagg agatcgccga
      241 gaagctgaac gtcacagtag agaccgagag aacccacatg gtgaacatac tccacaagct
      301 cggcgtgcac tcgcggttgc aggcccttgt cttcgcggcc cgctatggcg tcgtccggat
      361 acgctgaccc gccaatatga gagagcagcc cctgtacatg gtgccgacag acagatggct
      421 tccttgagcg cctgcgcagg ggaactgcag tcgcgcaagg cgctcatcct gctggcaaac
      481 gagccgaggg cgtaccggga gagcatcgcc caggtattgg gccagctacg cccgaacgtc
      541 gaggtgatta ccgcggagcc ggaggagctc gaagagcggg ttttgcgcct cgcccccgag
      601 atggtgatct tcagcgaggc gcccggcctc gtgcgcgagc gggtgccggt ctgggtcgag
      661 ttgtaccccg agtacggccg gcgctccgtg gtcggcgtgg gggaggagcg ctcgacgatc
      721 gaggagatac agctctccca cctgctctgc gtggtcgacc acgcggagcg tctagcccat
      781 tagggctaga aaaagttaac ccatttacgt gaggcggggc agctcggtcc tgagctatca
      841 tcgccagcaa tgaacggtgc gcgtgtattg gtggccctcg agctcacgtc ttaccgtgag
      901 gcgatcgcgg cggccttccg actgcttcga cctaacgtcg aggtcttcga ggccgaggat
      961 gaggacctcg agcgggaggt cgtacgcatc ggccctcacc tggtggtgtg cagccggctc
     1021 accgcctggg tcgagaatcg cgccccgaac tgggtagagt tataccccgg gtacagctcg
     1081 tactctgtgg tcagcgtgcg tggggagcgc tcgacgatcg aggagataca gctctcccac
     1141 ctgctctcgt tgatagacga gaccgtaaag aacggctgtt caggctaggg gtccgagagc
     1201 tcccttagaa gggtcttcag cctgggagcg atgtcatcta tctcgtcggc ggtgcactgg
     1261 acgtggaggt ggatcttgac agccgcgccc acctccacca tcgcggagac gacgggctcc
     1321 tccacatcct gggtatcggc ggagagctcg ttcgtagcga tcgtctccac cggatcgagc
     1381 tcaccgtcga aggtcgcgac cagctccccg gcctcctccc tgagaagccc tgcggtcttg
     1441 aggatctcca ctatggctcc cgcccccgtc atcaccggct tgttgcgcgg ctgtccggcg
     1501 gcgtgcgcga cgtacgcctg gacagtggga tagagcatgc cctcgcgcag cttcaccgcc
     1561 gcgaccacgt tctgcaggaa ctcgttggtg gcgacgatcg cgcgccagtt cctgcggatt
     1621 tcctcctcat ccttgcgtgc gagcgcgagc gccagggcgc gtccgcggcg ggtgatcaac
     1681 ttcttgctct cgccctggag gattcctatc tgggtcagga acccgttgtt gcggctgacg
     1741 ctcgactggt gcacggcgtc gagcttgccc acgtcgccgg gcctcgacgc ttcgtcgcgg
     1801 gtcccgtatg ccacgatgat gttcacgagc tcctcgtagg aggagcccgg gagccgaaac
     1861 tccctctgcg ccatccagta cctctcttcc tgacgctcca tccctctgac ccgcggccat
     1921 tatcctagat ttgacaatgc tttgccccgt cagggctcac gggccttctc gcgtccccgg
     1981 ttgatacaac ccacggcggc gtagtaccct ccgtgggttg tgagaagagg cagaccccgc
     2041 acgctaaccc gcagagagtt cctggcactc gcaggcgccg gggcgctcgg ggcgtccctg
     2101 ctcggcgcgg gctgcgacct gaccacgcga ctgccgaacc tccccggcca gccccccgcc
     2161 ggcgacacca acgtcgtcct ggtcatctta gacagcctga ggaaagacca catcggagcc
     2221 tacggcaacg actggatcca gaccccaaac ctcgacgctt tggctaaaga gagcctgcgc
     2281 ttcaccacag cctaccccga gtcggccccg accatctgcg cccgaagggc catccacaca
     2341 ggccttagga cctggccttt cagggactgg aagccgccta agggggagga cataatcctg
     2401 cagggctggc agccgatccc cgagggacag acgcacctgg ccgagataat gaagcagaac
     2461 ggctac
//
LOCUS       DASTUV010000079         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1672202, whole genome shotgun sequence.
ACCESSION   DASTUV010000079 DASTUV010000000
VERSION     DASTUV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672202"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2082)
                     /locus_tag="VFT03_01740"
     CDS             complement(<1..>2082)
                     /locus_tag="VFT03_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEU4846892.1"
                     /translation="LNGLHQCITTVLQQVRAADEDWPDMRERVREFVSEIDENRPPVE
                     DEDPVEARAFLEWISDDNFTFLGYREYDLLTEDGEDLLRSVPGTGLGILRETDQKPHS
                     LSFAKLPHEVRRLAHSPDILTLTKANSRATVHRPSYLDYVGVKRFDDEGDVSGERRFL
                     GLYDFSAYSMSVFDIPIVRRKVGYVLERAGFHEGSHNEKDLLEILETYPRDELFQIDK
                     EELFEIAMGILHLQERQRVRLFVRRDAYGRFFSCLVFVPRDRYNTRIREKMQEIFQRA
                     FDGTNVEFNVRLSESVLARLHFIVHVTPGENPDYDANEIEERLAEATRSWTDNLYDAL
                     IEHFGEERGTELFRKYRDAFPPGYRDGFLPRTAVADIARIEELESEEDIEMSLYHPLE
                     EPENFLGFKLFRLGEQTSLSGILPLLEDMGVEVVDERPHKIEPTGSQSGWIYDFGLLQ
                     EADGELQTGQVREIFQEAFARAWRGTVENDGFNRLVLRARLTWRQVTVLRAYCKYLRQ
                     AGTTFSQNYMEDALFANPHIARLLVELYEARFDPSRQARAEAENERLRGEIEAALDEV
                     VSLDEDRILRNFLNVTLATVRTNYYQRTPGGEPKPYLSFKFDPSEILVLPLPRPRFEI
                     FVYSPRMEGVHLRGGRVARGGIRWSDRREDFRTEVLGLMKAQMVKNAVIVPVGAKGGF
                     VVKRPPTQGGRE"
BASE COUNT          341 a          667 c          674 g          400 t
ORIGIN      
        1 ctcgcggcct ccctgagtgg gcggccgttt gacgacgaag ccacccttcg cgccgacagg
       61 cacgatcacg gcgttcttca ccatctgagc cttcatgagg ccgaggacct cggtgcggaa
      121 gtcctccctg cggtcggacc accggatgcc gccccgcgcg accctgcctc cccgcagatg
      181 cacgccctcc atgcgggggg agtagacgaa gatctcgaat ctcgggcggg gcagaggcag
      241 cacgaggatc tcgctcgggt cgaacttgaa ggagaggtac ggcttgggct cgccccccgg
      301 cgttctctgg tagtagttcg tgcgcaccgt cgccagggtg acgttcagga agttcctgag
      361 gatgcggtcc tcgtccaggc tcacgacctc gtcgagcgcc gcttcgatct cccccctcaa
      421 ccgctcgttc tcagcctcgg cgcgcgcctg acggctcggg tcgaagcggg cttcgtagag
      481 ctccacgagc aggcgggcga tgtgcgggtt cgcgaagagg gcgtcctcca tgtagttctg
      541 tgagaaggtg gtgccggcct ggcgcagata cttgcagtag gctcgtagca ccgttacctg
      601 gcgccaggtg agccgggcgc gcaggaccag gcggttgaac ccgtcgtttt ccacggttcc
      661 gcgccacgcc ctggcgaagg cttcctggaa gatctcccgc acctggcctg tctggagctc
      721 gccatccgcc tcttgcagga ggccgaagtc gtagatccag cccgattgag agcccgtcgg
      781 ctctatcttg tgcgggcgtt cgtctaccac ctcgacaccc atgtcctcca gcagaggcag
      841 gatgcctgag agggaggtct gctctcccag cctgaacagc ttgaagccga ggaagttctc
      901 cggctcctcg agcgggtggt agaggctcat ctctatgtct tcctctgatt cgagctcctc
      961 gatcctggcg atgtccgcca cggcggtccg gggcaggaaa ccgtcacgat agcccggcgg
     1021 gaaggcgtcc cggtacttgc ggaagagctc ggtgccccgc tcctccccga agtgctcgat
     1081 cagggcgtcg tagaggttgt ccgtccacga gcgggtcgct tcagccaggc gctcctcgat
     1141 ctcgttggcg tcgtagtccg ggttctcacc cggcgtcacg tggacgataa agtgtaggcg
     1201 cgccagcacg gactcagaga ggcggacgtt gaactcgacg ttggtgccgt cgaacgcccg
     1261 ctgaaagatt tcctgcatct tctccctgat gcgcgtgttg tagcggtcgc gcggcacgaa
     1321 aaccagacaa gagaagaagc gcccgtaggc gtcgcggcgc acgaagagcc tcacgcgctg
     1381 gcgctcctgg aggtggagga tgcccatcgc tatctcgaag agctcctcct tgtcgatctg
     1441 gaacagctcg tcgcgcgggt aggtctcgag gatctcgagc aggtccttct cgttgtggct
     1501 gccctcgtgg aaccccgcgc gctctagcac gtagccgact ttgcggcgga ctatggggat
     1561 atcgaagaca ctcatgctat atgccgagaa gtcgtagagg cccaggaagc gccgctcgcc
     1621 ggatacgtcg ccctcgtcgt cgaacctctt cacgccgacg tagtcgagat aggaaggccg
     1681 gtgcacggtc gcccgcgagt tcgccttggt cagggtgagg atgtcgggcg agtgggcaag
     1741 ccttcgcacc tcgtgcggca gcttggcgaa gctgagcgag tggggcttct ggtccgtctc
     1801 ccgtagtatg ccgagacccg tccccggcac ggaccttagc agatcctccc catcctcggt
     1861 aagcagatcg tactcgcggt agcccaggaa agtgaagttg tcgtcggaga tccattcgag
     1921 gaaagccctg gcttccaccg gatcttcatc ctccacgggc ggccggtttt cgtctatctc
     1981 cgagacgaac tcgcgcaccc tctcgcgcat gtcgggccag tcctcgtcgg cggcgcgcac
     2041 ctgctgcagg acggtggtga tgcactggtg gagcccattt ag
//
LOCUS       DASTUV010000080         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_419391, whole genome shotgun sequence.
ACCESSION   DASTUV010000080 DASTUV010000000
VERSION     DASTUV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419391"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..965
                     /locus_tag="VFT03_01745"
     CDS             105..965
                     /locus_tag="VFT03_01745"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEU4846893.1"
                     /translation="MSEYGSGWTSRAVLITGCSSGIGWATAERLSQRGWTVYATARNV
                     EEISPLVQSGCRLLPLDVTDEESMRHAVEEVERIEGAVGVLINNAGYSQSGAIEEVPM
                     EKVRRQFETNVFGLVRMCQLALPGMREQGFGRIVNLSSMGGRLTFPGGGFYHASKHAV
                     EAVSDALRFEVRDFGVDVVVIEPGLIRTGFVQTAVGSIGEGSWPYAGFEAGVARATRE
                     NYERGPLAALGGGPETVAEAIERALSTARPRSRYTVTPSAKLLISARRLLPDRVWDAL
                     VGLFYPRPGG"
     gene            1140..2039
                     /locus_tag="VFT03_01750"
     CDS             1140..2039
                     /locus_tag="VFT03_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846894.1"
                     /translation="MTEALLLIVVGSLVPISVATLIFALLTLRKAHLYVVLAEQRFEH
                     LREGQVVLLALLREQSRSSETTGQVHRAQEPVLREREPLTAAVRTRGIDELGLHGPLW
                     NAGRGADDELGVGSSLPDWPPEDTPGARERADKEEEPPKEEPPKERGSESRPRAANFP
                     DTTSSSRPAKAAPDAGVSHRAVWHPHPDDDVSPARVGGPSGVTVKMFRMHYDKYLDNY
                     EGYVKLAARLFRMRDDAEIEAGSAAEGEWDERLRRVTDGIQRTTARLDILEEYNPELA
                     TDDRISRRAAIARDQRELELQRR"
BASE COUNT          399 a          665 c          782 g          347 t
ORIGIN      
        1 cgacggtatc tacctcgcgg cgacggtagt ctacgcccgg cgcgagcgaa gcgcgaagcg
       61 gcataatctc cggtaacggc cggcggcaag ggacaggggg attgatgtcc gagtatggat
      121 ccgggtggac ttcgagggcc gtgctcatca ccggctgctc gtccggcatc ggatgggcga
      181 cagcggagcg cctatcgcag aggggctgga ccgtgtacgc cacggcgcgc aacgtcgagg
      241 agatctcgcc gctggtgcag agcgggtgca ggctgctccc gctcgacgtt accgacgagg
      301 agtccatgcg gcacgccgtc gaggaggtcg agagaataga gggggcggtg ggggtcttga
      361 tcaacaacgc cggctacagc cagtccggcg cgatcgagga ggtccccatg gagaaggtgc
      421 ggcgccagtt cgagacgaac gtcttcgggc tcgtgcgcat gtgccagctc gcgttgccgg
      481 ggatgagaga gcagggcttc gggaggatag tcaacctctc ttccatgggt ggcaggctca
      541 cctttcccgg aggaggcttc tatcacgcga gcaagcacgc cgtcgaggcc gtaagcgacg
      601 ctctgcgctt cgaagtgcgc gacttcgggg tcgatgtcgt ggtgatcgag cccggcctga
      661 tccgcaccgg tttcgtccag acggcggtag gttcgatagg ggaggggagt tggccctacg
      721 ccggcttcga ggcgggtgtg gcgcgggcga cgcgcgagaa ctacgagaga gggccgctgg
      781 cggccctggg cggaggacct gagaccgtcg ccgaggcgat agagcgtgcg ctctcgaccg
      841 cgcgaccgcg ctctcgctac accgtcacgc cctcggcgaa gctactgatc tccgcgcggc
      901 ggctcctgcc ggaccgggtc tgggacgccc tcgtgggact cttctacccc cggcccggcg
      961 ggtagcgcag cattccagag agagcttgcc ggcccgaggg ctgcgggggc gaaatcgtgg
     1021 ctattcgaag atttttttca cttttgcctc agaaaagtgc tgctaagggc caatcatccc
     1081 ttgccatccg gctacgactg ctgttatcgt agggtagtca cttgagggag gcgcagggtt
     1141 tgacggaagc tctacttctg atagtagttg gttctctagt tccgatcagc gtagccaccc
     1201 tcatattcgc ccttctcacc ctgcgaaagg cccacctcta cgtggttctg gccgagcagc
     1261 gcttcgagca ccttcgcgag gggcaggttg ttctcctcgc gctactgcgc gaacaaagcc
     1321 gaagctcgga gacgacagga caagtgcatc gcgcgcagga gccagtcctc cgggaacgcg
     1381 agccacttac ggcggccgtg aggaccaggg ggatagacga gctcgggctc cacggtcccc
     1441 tctggaacgc cggaagaggc gcagacgacg agctgggagt cggttcgtcc ctgccggatt
     1501 ggccgcccga ggacacacct ggggcgcgcg agcgcgcgga caaagaggag gagccgccga
     1561 aggaggagcc gccgaaggag cggggctccg aaagccggcc gcgcgcggcg aacttccccg
     1621 acaccacgtc gtcctctagg cccgcgaagg ccgccccgga cgccggggtg tcgcaccgcg
     1681 cggtgtggca cccgcacccc gacgacgacg tcagcccggc gcgggtgggc ggaccgagcg
     1741 gcgtgacggt caagatgttc cgcatgcact acgacaagta tctggacaac tacgagggtt
     1801 acgtgaagct ggcagcgagg ctcttccgga tgcgggacga cgctgaaata gaagccggtt
     1861 ccgcggcgga gggcgagtgg gatgagaggt tgcgcagggt gaccgatggc atccagagga
     1921 ccacagcgag gctggacatc ctggaagagt acaaccccga gctggcgacc gacgaccgca
     1981 tctctcgccg cgccgccata gcccgggacc agcgggagct cgagctccag aggcggtagc
     2041 gccagcagat cgagggatcg ggcttttcag agcatctgtg gttcactggc tccacagcca
     2101 gcagctgagc aggcgatagc cgacaatacc ttcgtacgga tctgggccat gtcgtaggca
     2161 tgcccgccac cgtcggctag acggaatcgt ggg
//
LOCUS       DASTUV010000081         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1600459, whole genome shotgun sequence.
ACCESSION   DASTUV010000081 DASTUV010000000
VERSION     DASTUV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600459"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /gene="rpmB"
                     /locus_tag="VFT03_01755"
     CDS             complement(<1..153)
                     /gene="rpmB"
                     /locus_tag="VFT03_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU4846895.1"
                     /translation="MAKVCYSCGKKPSYGNARSHSLRATRRRWNPNLQKVRIQEGNST
                     RREWICT"
     gene            343..1926
                     /locus_tag="VFT03_01760"
     CDS             343..1926
                     /locus_tag="VFT03_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU4846896.1"
                     /translation="MAVTLEPLVAAAHAALEAKVARINALNVYPVPDGDTGTNMLLTL
                     ESVLKEASDKTYEGLEEATKAGARAALMGARGNSGVILSQMIRGGCEVLGDSDSLSAE
                     TVAAALEGACERAYASVRDPVEGTMLTVMRDAAHAAHAALEVEDASIPAVIQAAAREA
                     HASVGRTPELLEVLRDAGVVDAGGLGVAVILDGLHAFVTGQEVEVPEVEGGVPDLEAI
                     HAEEEPWGYCTEFVVTGFSGDAREFEERIQSSGKSVLVVSDEDLVKVHLHTQDPGDAL
                     SYAGRFGRLSGVKVDDMEDQVRSRGDSSGAAEQKDLGVVAASRGEGSRELFERMGAVV
                     IEGGQGENPSAADFARAVEKTGARFVILLPNNKNIVPTAEQVHELVDGVEVHVVNTMS
                     MAAGLAAMVGFDPEGEAGEVLDEMREITAGLRCAEVTQAVREASIDGHKVPEGAYIGL
                     LDGKLVAVGDGVEDAAIKLAERMLEGGVDIVTLLRGYGMDQKSAERVAEGIKHLDGDV
                     EVEIKDGGQPLYPLQMVAE"
     gene            1926..2741
                     /locus_tag="VFT03_01765"
     CDS             1926..2741
                     /locus_tag="VFT03_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU4846897.1"
                     /translation="MTTAVVTDSTTSLAPGVLERPDFRMVPLTFHFGPERTYRDKIDM
                     SNEEFYERLDAADEFPTTSQPAPGSFVEAFEALEAYDHILVLTVSRKFSGTYDSAVSA
                     AGMVDRPVEVLDTRSAEMGSGLILMEALRVVDEGGDFKNVRRAAESAIGRANLLFAVG
                     SLEYLAKSGRLGRARRLVGAALDIRPVLRVEEGEVVPHKRARGRKRQMAAIVEEVRPA
                     VEAGRQLYFGHVAAPELLSELADQLGVEEQLVSEIGGVVGSHVGPGAYGVAHL"
     gene            2722..>3156
                     /locus_tag="VFT03_01770"
     CDS             2722..>3156
                     /locus_tag="VFT03_01770"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU4846898.1"
                     /translation="MAWPTSSVEAAYTPPQRTTLPDLRSRPVTELPGVGPRIQAALEE
                     LGVASLADMISHYPSRHEDLSNVKKISDLRVGERATVVARVVSTRPVGRPVRGRSPGF
                     SAQLYDGTGYLPATVWGRGWLLNQLLPDTRVVVSGEVQRRYGI"
BASE COUNT          527 a          962 c         1174 g          493 t
ORIGIN      
        1 ggtgcagatc cactcgcgcc tggtcgaatt gccttcctgg atgcgaacct tctgcaggtt
       61 ggggttccag cggcggcgcg tcgcccgcaa agagtggctc cgggcgttgc cgtagctcgg
      121 cttcttaccg cacgaataac agactttagc cacgatataa ttctcctgtg ctaggatacc
      181 ggtcgaattt ggggacccgc gcggccctgg cgccgtcggc gagtatcggg aggagattgt
      241 accagattct cgggctatac acggcccgcg gggcttgagg gctccagtgt gaccgcgcgc
      301 caggagagcg gattcggagc gcagcgtgag aggtccgatg cgatggcggt aacgttggag
      361 cccctggtgg ccgcggcgca cgcggcgctc gaggccaagg tcgccaggat caacgcgctc
      421 aacgtctatc ccgtccctga tggcgacacg ggaaccaaca tgctccttac tctggaatcc
      481 gtcctcaagg aggcctcaga caagacgtac gaagggctcg aggaggccac gaaggctggc
      541 gcccgggcag cgctcatggg ggcgagggga aactcggggg tcatcctgtc gcagatgatc
      601 cggggcgggt gcgaggtgct cggcgatagt gattctctga gcgccgagac cgtggcggcc
      661 gcgctcgaag gggcttgcga gcgggcttac gcttcggttc gcgaccccgt agaggggacc
      721 atgctcaccg tcatgcggga cgccgcgcac gccgcgcacg cggcgctcga agtggaagat
      781 gccagtatac cggccgtgat ccaggccgcg gcccgcgagg ctcacgcttc ggtgggccgc
      841 acccctgagc tgctcgaggt cctgcgcgac gccggggtgg tggacgcggg gggcctgggt
      901 gtggccgtga tcctcgacgg cctccacgcc ttcgtcacgg gacaggaggt cgaggtgccg
      961 gaggtggagg gaggcgtccc cgacctggaa gcgatccacg ccgaggaaga gccctggggt
     1021 tactgcaccg agttcgtcgt gaccgggttc tccggggacg cgcgtgagtt cgaggagcgc
     1081 atccagtcct ccggcaagag cgtgctcgtg gtctccgacg aagacctcgt caaggtccac
     1141 ctgcacaccc aggacccggg cgatgccctg tcttacgcgg ggcggttcgg gcgtctctcc
     1201 ggcgtaaagg tcgacgacat ggaggaccag gtgcggtcca ggggtgacag ctccggtgct
     1261 gccgagcaga aggatctcgg cgtagtggcg gcgagccggg gtgagggtag ccgcgagctc
     1321 ttcgagcgga tgggcgcggt ggtgatcgag ggtgggcagg gcgagaaccc gagcgccgca
     1381 gacttcgcca gggccgtgga gaagacgggc gcgcgcttcg tgatcctgct tcccaacaac
     1441 aagaacatcg tgcccacggc ggagcaggtg cacgagctcg tggatggcgt agaggtccac
     1501 gtcgtcaaca cgatgagcat ggcggcgggg ctcgccgcga tggtgggctt cgaccccgag
     1561 ggggaggccg gggaggtcct cgatgagatg cgcgagatca ccgcgggcct gcgttgcgct
     1621 gaggtgacgc aggccgtccg cgaggcctcg atcgacggtc acaaagtgcc ggagggcgca
     1681 tatatcggcc tcctggacgg gaaactggtc gccgtgggag acggcgtgga ggacgcggcg
     1741 attaagctcg ccgagaggat gctggagggc ggtgtggaca tcgtgacgct gctcaggggc
     1801 tacgggatgg accagaagtc agccgagagg gtcgccgagg ggataaagca ccttgacggt
     1861 gacgtggaag tagagatcaa agacggcgga cagccgctgt atccgctgca gatggtggcc
     1921 gagtagtgac gaccgccgtg gtaacggact ccacgacgag cctggcgccc ggcgtgctcg
     1981 agcgaccgga cttcaggatg gtgccgctca ccttccactt cggcccggag cggacctacc
     2041 gcgacaagat agatatgtcc aacgaggagt tctacgagag gctagacgcc gcagacgagt
     2101 tcccgactac atcccagccc gcgccgggga gcttcgtcga ggcgttcgag gcgctggaag
     2161 cctacgacca catcctcgtg ctcaccgtct cccgaaagtt cagcggaacc tatgactccg
     2221 ccgtctccgc ggctggtatg gtggaccggc ccgtagaggt gctcgacacg aggagcgccg
     2281 agatggggtc gggtttgatc ctcatggagg cgctgcgcgt cgtggacgag ggcggggact
     2341 tcaaaaacgt acggcgcgcg gcggagagcg ctatagggcg cgccaacctc ctgttcgccg
     2401 tgggctccct ggagtacctc gcgaagagcg ggcgtctggg gcgggcgcgg cgcctcgttg
     2461 gagccgccct cgatatcagg cccgtcctgc gggtcgagga aggtgaggtc gtcccccaca
     2521 aacgggcgcg cggccgcaag cgtcagatgg cggcgatagt cgaggaggtc aggcccgccg
     2581 tcgaggcagg ccggcagctt tacttcgggc acgtcgcggc ccccgagctc ctctccgagc
     2641 tggccgacca gctcggggtg gaggagcagc tcgtttcgga gataggcggg gtcgtcggct
     2701 cccacgtcgg gccgggggcg tatggcgtgg cccacctcta gcgtcgaggc ggcctacacg
     2761 ccgccgcaga ggacgacgct gcccgacctg cgctcgcgtc ccgtcacgga gctaccaggc
     2821 gtcggcccgc ggatacaggc ggcactcgag gagctaggcg tagcctcgct tgcggacatg
     2881 atctcccact atccctcgcg ccacgaggac ctctcgaacg tcaagaagat ctccgatctg
     2941 cgcgttggcg agagggcgac ggtggtcgcc cgcgtcgtga gcacccgtcc ggtgggcagg
     3001 ccggtgcggg ggcgctcccc cggcttctcc gcccagctct acgacggcac aggctacctg
     3061 ccggcgacgg tctggggacg gggttggctg ctgaaccagc tgttgcccga caccagggtc
     3121 gtcgtctctg gggaggtgca gcgccggtac gggata
//
LOCUS       DASTUV010000082         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_742119, whole genome shotgun sequence.
ACCESSION   DASTUV010000082 DASTUV010000000
VERSION     DASTUV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742119"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFT03_01775"
     CDS             complement(<1..702)
                     /locus_tag="VFT03_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4846899.1"
                     /translation="MLLENKNAVIYGAGGIIGGAVARKFAHEGARVFLAGRTREKLDA
                     VAEGIEAAGGSAEVAVLDALDEGAVDEHADAVFTQAGSIDVSFNLITHPSVHGTPLAE
                     MAVEDYMVSVTTAVRTTFLTAKAAARPMISQGAGAILFFGGSGDPIRDYFIGGTQVAF
                     DAVESMRRQLAVELGPHGIRTVTLKTGGIPESIPEGIEGREEIVEKHLVGPTMLGRAA
                     TLEDVGNVAAFVASDH"
     gene            complement(1034..1537)
                     /locus_tag="VFT03_01780"
     CDS             complement(1034..1537)
                     /locus_tag="VFT03_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846900.1"
                     /translation="MGAKTRKGILVVHIASAGVWIGIDVVMGVLVFTALFGNEETRAL
                     CYRALELFAVWPLIATGLVSLASGIVLGLGTKWGLVRYWWVAIKLALNIILVALVPVA
                     LRPEVIEKAEQGRRFVAGEAASLAVGDLIFPPIVSPTLLLIAVVLAVFKPWGRLRGRR
                     EPKRGAR"
     gene            complement(1581..1997)
                     /locus_tag="VFT03_01785"
     CDS             complement(1581..1997)
                     /locus_tag="VFT03_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4846901.1"
                     /translation="MSTKRALIHVLLRSEQSAGVVSMIESGSLPDFGGPPLHHHDFDE
                     TFYVMEGELTFQLGDELFSAKAGELTFAPGRRAARVGAAADPRGHHRRSADRRAGHQG
                     STKRPEGAIFMTESCREHPPTPDSEVRATAPGVEGR"
BASE COUNT          383 a          788 c          699 g          408 t
ORIGIN      
        1 gtggtccgag gccacgaagg ccgccacgtt gcccacgtct tcgagggtcg ccgcgcggcc
       61 cagcatcgtt ggccccacga gatgcttctc gacgatctcc tcccgtccct cgatgccctc
      121 ggggatcgac tccggtatgc cgccggtctt cagggttacc gtgcggatac cgtgcgggcc
      181 gagctcgacc gccagttgcc gccgcatgga ttcgaccgcg tcgaaggcga cctgggtgcc
      241 tccgatgaaa tagtcgcgta tgggatcgcc cgagccaccg aagaagagga tcgctcccgc
      301 tccctgagag atcatgggcc gcgccgccgc cttcgcggtt agaaaggtcg tccttacggc
      361 ggtcgtcact gacaccatgt agtcctcgac cgccatctcg gccagcgggg tcccgtgcac
      421 ggaagggtgc gtgatcaggt tgaaggagac gtcgatgctg cccgcttgcg tgaacacggc
      481 gtcggcgtgc tcgtccacgg ccccctcatc cagggcgtcg aggaccgcga cttcggctga
      541 cccgcctgcg gcctctatgc cctcggcgac cgcgtcgagc ttctcgcggg tgcggccggc
      601 gagaaagacc cttgcgcctt cgtgggcgaa cttcctggcg acggcgccgc ctattatccc
      661 tcctgcaccg tagatgacgg cgttcttgtt ctccagcaac atcctcatcc tctcctttcg
      721 gccatttgtt gatcggagcg ccagagccta cgttggcggc catcattgtg cgggcagagg
      781 cgaagatcgc cactttgcca acgtacttga gcgtcgccgc ctgctctctt agacctcaca
      841 tcagtcacct cccagctcat agcgaacgtt tggagcgggg gatcggcccc aggctgagac
      901 acccgccgcg gccacgtcga agagcgaggg atagtcgctc tgcggccacg aggtcgaatc
      961 ggcgctcgaa gcccgtcggc gagcagacga gtacgtatcg tgcctcggcc gcggatcccc
     1021 tcgttggcgc cgctcatcgc gccccccgct tcggctcccg gcgcccgcgg agtcggcccc
     1081 acggcttgaa caccgccaac accaccgcga tgagcaggag ggtcggggag acaatcggag
     1141 ggaagatcag gtcgcccacc gccagtgagg ccgcctctcc ggccacgaac ctcctcccct
     1201 gctcggcctt ctcgatcacc tccggccgca acgctaccgg gaccagcgcg acgaggatta
     1261 tgttcagcgc tagcttgatc gccacccacc agtacctgac aaggccccac ttggtgccca
     1321 agcccaggac gatgccgctc gcgagggaca cgagaccggt agcgatgagc ggccagacgg
     1381 cgaacagctc cagcgcccgg taacagagcg ccctcgtctc ttcgttaccg aagagggcgg
     1441 tgaagacgag gacgcccatg acaacgtcga tgccgatcca gaccccggcc gaagctatgt
     1501 gcacgaccag gatgcccttg cgcgtcttcg cccccaaccg ccatttctga gtaccgcctc
     1561 gtgcaagggg cgtcatcaca ctaccttccc tcgacgccgg gcgccgtggc ccgaacctcg
     1621 gagtccggcg tgggcggatg ctctctgcag ctctcggtca tgaagatcgc cccctccggt
     1681 cgcttggtcg atccctgatg tcccgctcgc cgatccgcgg accgacggtg gtggcctcgg
     1741 ggatcggctg cagcgcccac tcgggcggct cttcgccccg gcgcgaaggt gagctctccg
     1801 gctttcgcgc tgaagagttc gtcgccgagc tgaaaggtca gctcaccctc catgacgtag
     1861 aaggtctcgt cgaagtcatg gtggtgcagc ggcggcccac cgaaatcggg gagtgacccg
     1921 ctctcgatca tggagaccac gcccgcgctc tgctcgctgc gcagaaggac gtggatcagg
     1981 gcacgcttcg tagacatatc cgttcttcct cctaggatct attgggtgcc tccgatcgta
     2041 tgcgaacccg ggcgtagacg catccaccga aagttagaga agggggttag agatctatct
     2101 ctagccttgc ggtcccagac cgcgcctccc tgcgatacct tgtctgagct cgtccgagct
     2161 ctcatgtcga tcctcgcagc agtaggccaa gtatctgttt gggcatccgg tccacggtca
     2221 cgccatcgac cggcggtcag gaggtaagga ccaggctatc tgcacagtgc ggattcga
//
LOCUS       DASTUV010000083         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1492747, whole genome shotgun sequence.
ACCESSION   DASTUV010000083 DASTUV010000000
VERSION     DASTUV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492747"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFT03_01790"
     CDS             <1..925
                     /locus_tag="VFT03_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU4846902.1"
                     /translation="ADVSDKPDYVRGLSPLSASEISALDSGYRRGLRSLMRVDRFVGD
                     ASDLLRRKGEMSNTYFLFYTDNGAHFGQHRFEHGKLQPYEEDVNFPLFVRGPGTPHGA
                     IKPELVGTHDIAPTLAKMAGADVPNFVDGRSFLPLATGAVTSWPPHGDPQREGDRRRP
                     TQQMGYAAHEGQPGGQGLHPQRERGGQQGVLGPSGRPLPGAQCSRPVRYRLSGARFWN
                     QGVLRAALGRPLPLQRPVVPDGRERPTLPVTFRALGQKLEEWSRMRGHADPQEIESID
                     ERRWESLRPAHYQTESALEALMGGDAPPRRA"
     gene            1163..>2335
                     /locus_tag="VFT03_01795"
     CDS             1163..>2335
                     /locus_tag="VFT03_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846903.1"
                     /translation="MSKVQPEKSNLDDSAVMDSITSQWWSFSAFVPLLTGTLWLWLAS
                     RSGLPAVLLGAVPGALLLGTGLSSLLWAVESRTFQYMALASALGGLLSLPAILLFGPL
                     AALVLELGSAVSFLATGYLALGQHRCPSGVPAPDTGPRIALRAAVNELMMCLLMLTSW
                     PIVVGTIATRVRREATETYRMFEDEGWFSDPAAYHRNPPPLEDPNISTINHRGREIEC
                     LTFESLYEPRPEEPGRERWLSYKRNSTAHAWILRHHDEPRPWLICVHGIRMGTLNKSL
                     MRFQPEYLHEELGLNLLMPVLPLHGPRDTGLISGERTLSGDVMDTLHTGEQAIWDLRR
                     LVWWLRESEGASAIGALGHSLGGYTVSLLASLEDLDCVVAGNPAVNPSHLFWTDALA"
BASE COUNT          425 a          761 c          734 g          415 t
ORIGIN      
        1 ggccgacgtc tctgacaagc ccgactacgt gcgcgggctg tcccccctct ccgcttcgga
       61 gatctccgcg ctggactcgg gctacaggcg ggggttgcgc tccctgatgc gggtggaccg
      121 cttcgtcggg gacgcctcgg acctgctccg ccggaagggc gagatgagca acacctactt
      181 cctgttctac acggacaacg gggcccactt cggccagcac cgtttcgagc acggcaagct
      241 ccagccctac gaggaggacg tcaacttccc cctgttcgtg cgcggccccg gcaccccgca
      301 cggcgccatc aagccggagc tcgtgggcac ccacgacatc gcgccgaccc tggcgaagat
      361 ggccggcgca gacgtcccga acttcgtgga cggtcgcagc ttcctgcctc tcgccacggg
      421 cgcggtcacc tcctggcccc cgcacggcga tcctcagcga gagggagacc gacgccgacc
      481 cacccaacag atgggatatg ctgcgcatga ggggcagcca gggggtcaag gtctacaccc
      541 gcaacgagaa cggggtggtc aacaaggagt actaggacct agcggtcgac cccttccagg
      601 tgcacaatgc tctcggcccg tcagataccg cttatccggc gcccgattct ggaaccaggg
      661 cgtactacga gcagcgcttg gacgacctct accgttgcag cggccagtcg tgccggacgg
      721 ccgagagcgc cccactctcc ccgtaacgtt ccgtgctcta gggcagaaac ttgaggaatg
      781 gagcaggatg cgtggccatg ccgaccccca ggagatagag tcgattgatg aacgcaggtg
      841 ggagagccta aggccggccc actaccagac cgagagcgct ctcgaagcgc tcatgggagg
      901 tgacgctccg cctcggagag cataggcgcc ggccaacgtt agtatgcggg ctcttaccgg
      961 gggcattcgg atccatcaac tccggttccc tgcacttccg ccccgctgta acgagttgtg
     1021 acgggttgct ccataatctt aggaatctgc gtcaatcgga gcgtcgatga atggccagaa
     1081 ggtctacgat ggtgtccctg tgagcgtcgg ttcatctaga atgagccggt cgtgcctact
     1141 catgcctggg aaagggtggt cgatgagcaa agtgcagccg gagaaaagca acctggacga
     1201 cagcgcagtt atggattcca taacgtcgca gtggtggtct ttctcagcat tcgtcccatt
     1261 gctgacgggt acgctctggc tgtggctggc gtcacgcagc ggcctgccgg cggtcttgct
     1321 cggagccgtg ccgggagcac tgttgctcgg caccggactc tcgagccttc tatgggcagt
     1381 cgaatccagg acgttccagt acatggcgct ggcctcagcc ctcgggggac tactctcgct
     1441 acccgcgatc ctgctcttcg gaccgctggc cgcgctcgta ctcgagttgg gttcggcggt
     1501 gagctttctt gcgacgggct atctggccct ggggcagcat cgctgtcctt ccggcgtgcc
     1561 ggcacccgat acgggtccta ggatcgcctt acgggccgcg gtgaacgagc tcatgatgtg
     1621 cttactcatg ctcacgagct ggccaatcgt ggtgggcacg atcgccaccc gggtccggcg
     1681 cgaggccacg gagacctacc ggatgttcga agacgaaggc tggttctcgg atcccgccgc
     1741 gtaccatagg aacccaccgc cgctagaaga tcccaatatc agtacaatca accacagggg
     1801 aagggagatc gagtgcctga ccttcgagag tctctacgag ccgcgtcccg aggagcccgg
     1861 acgcgagcgc tggctctcct acaaacgcaa ctccaccgcg cacgcctgga tcctacgcca
     1921 ccatgatgag ccgcgcccgt ggctgatctg cgtccatggc ataaggatgg gcaccctgaa
     1981 caagagcctc atgcgatttc aacccgaata cttgcacgaa gagctggggc taaacctgct
     2041 catgcccgtg cttccgctgc acggcccgcg cgataccggc ctcatcagcg gcgaacggac
     2101 actctcagga gacgtaatgg acacgctgca cacgggggag caggcgatct gggacctcag
     2161 gcggcttgtc tggtggctgc gtgagagcga gggagcctcg gcgatcggag cgcttggcca
     2221 ctctctgggc ggctacaccg tctcgctgct cgcctctttg gaagatttgg actgcgtggt
     2281 cgcgggcaac ccggcggtga acccctccca cctgttctgg accgacgcgc tcgcc
//
LOCUS       DASTUV010000084         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_813253, whole genome shotgun sequence.
ACCESSION   DASTUV010000084 DASTUV010000000
VERSION     DASTUV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813253"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..438)
                     /locus_tag="VFT03_01800"
     CDS             complement(166..438)
                     /locus_tag="VFT03_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU4846904.1"
                     /translation="MARLRQIFVCATPGEGRCGAKGGGELLEAFRAEVERRGLRPAAV
                     LRNACARRHHEGPVVFVYPDDVWYTRVALEDVSDIVERHLAPAGVT"
     gene            complement(692..2137)
                     /locus_tag="VFT03_01805"
     CDS             complement(692..2137)
                     /locus_tag="VFT03_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU4846905.1"
                     /translation="MEAIPSASYSFTLRVEFPHRAGSLGKILTAVGDAGAMVGAVDIV
                     RMGQERSTRDITVNARDSKHGQQVVDVVEGLPEVTVINVSDRTFLMHLGGKIEVRSRM
                     QLRTRDDLSMAYTPGVARVCRAIADDPERAFNLTIKRNTVAVVSDGTAVLGLGDIGPR
                     AAMPVMEGKAMLFKEFAEVDAFPICLDTKDADEIVETVKNLAPTFGGINLEDISAPRC
                     FEIEDRLKEELDIPVFHDDQHGTAVVVLAALINSLKIVGKKIEDLRIVVNGVGASGVA
                     CAKIMLEAGATNIVGCDSQGIVHAGREGLNPSKQWFAEHTNPDGRTGDLSDAVRDADL
                     FLGLSVPGVLTVEHLESMNKDPLLFVMANPDPEIRPEVALGHARIIATGRSDYPNQIN
                     NVLCFPGIFRGALDVRAREINEDMKLAAAQAIADVIPEEELSEDYIIPSVFDERVASA
                     VAEAVGEKAKETGVARRVQERTETGLSATAF"
     gene            2424..2669
                     /locus_tag="VFT03_01810"
     CDS             2424..2669
                     /locus_tag="VFT03_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU4846906.1"
                     /translation="MRRCCASIPTNIAEGCGRTGEAELARFMLISMGSASELEYHLLL
                     ARDLGYLSAPHYQELLQEAGEVKRMLSTFITKLRHTK"
BASE COUNT          481 a          876 c          822 g          503 t
ORIGIN      
        1 cgcccaggcc ctctacagga gcgtactcct ctactacctc aagagccgcc gggaccgcat
       61 cctcgccgtc tccgactggg cgatggagca aaccctcggg cgcgtgggat tcgaagtcat
      121 caggccacag gcttcagcaa gaacccgtaa cctgaaacct gagacctaag tcaccccggc
      181 gggcgccagg tgccgctcga cgatgtctga tacgtcctct agtgcgacgc gggtgtacca
      241 gacgtcgtcg gggtagacga agacgaccgg tccttcgtgg tggcggcgcg cgcaggcgtt
      301 gcgcagcacc gcggccggcc gcaggccgcg ccgctcgacc tcggcgcgga aagcctcgag
      361 gagctcgccg ccgcccttgg cgccgcagcg gccctccccg ggcgtcgcgc acacgaagat
      421 ctgtcgaagc ctcgccacga caccaattct acgtcgcacc gggcattatt tcaggcgcgg
      481 acctgtcgcc tacaccaatt ctcgaaatga tcgatcccaa caagaaccca ggagagcctg
      541 ccccccgaaa caataagctg acaagcgagg ccctaaaagg gccgaagcgc gctgacaagg
      601 cgcgaagcgc cgtgctgaca agccgaccga agggaggcgt gctgacaagc gacagccgaa
      661 ggctggagcg tgctgaattg cgcgaagcgc cttagaaggc ggtcgccgag agtcccgtct
      721 ccgtgcgctc ctgcacgcgc ctggccacac cggtctcttt ggccttctct ccgaccgcct
      781 cggcgacggc ggaggcgacg cgctcgtcga agacgctcgg gatgatgtaa tcctcggaga
      841 gctcctcctc ggggatcacg tcggctatcg cctgggccgc ggccagcttc atgtcctcgt
      901 ttatctcgcg ggcgcgcacg tcgagggcac cacggaagat cccagggaag cacaggacgt
      961 tgttgatctg gttcgggtag tcgctgcggc ccgtggcgat gatcctggcg tgccccaagg
     1021 ccacctcggg ccttatctcc gggtcggggt tggccatcac gaacagtagc gggtccttgt
     1081 tcatgctctc caggtgctcg acggtaagca cgccagggac agagagcccg aggaagaggt
     1141 cagcgtcccg caccgcgtct gagaggtctc ctgtcctgcc gtccggattg gtgtgctcgg
     1201 cgaaccactg tttggatggg ttgagcccct cgcgccccgc gtgcacgatg ccctgcgagt
     1261 cgcacccgac tatgttcgtg gcgccggcct cgagcatgat cttggcgcac gctacccccg
     1321 aggccccgac gccgttcacc acgatccgga ggtcctctat cttcttgcct acgatcttga
     1381 gcgagtttat gagcgcggcg aggacgacga ccgccgtgcc gtgctggtcg tcgtggaaga
     1441 cggggatgtc gagctcctcc ttgaggcgat cctcgatctc gaagcaccgc ggagccgaga
     1501 tgtcctcgag gtttatcccg ccgaaggtcg gggccaggtt cttgacggtc tccacgatct
     1561 cgtccgcgtc cttggtgtcg aggcagatgg ggaaggcatc tacttcggcg aactccttga
     1621 agagcatcgc cttgccctcc attacaggca tcgcggcccg tggcccgatg tccccgaggc
     1681 cgagcaccgc cgtgccgtcg gagacgaccg ccacggtgtt gcgtttgatc gtgaggttga
     1741 aagccctctc ggggtcgtcg gctatcgcgc ggcagaccct ggccacacca ggtgtatagg
     1801 ccatagagag gtcgtcgcgg gtcctcagct gcatcctgga gcggacctcg atcttgcctc
     1861 ccaggtgcat taggaacgtc cggtcggaga cgtttatgac cgtcacctcg ggcagccctt
     1921 ccacgacatc cacgacctgc tggccgtgct tggagtcgcg cgcgttgacc gtgatgtcgc
     1981 gggtgctgcg ctcctgcccc atgcgcacta tgtcgacagc gcccaccatc gcccccgcat
     2041 cgccaaccgc cgtcaggatc ttgccgagcg accccgcccg gtgaggaaac tcgacccgca
     2101 aggtgaaact gtagctcgcg cttggtatgg cttccatgat gttcgttcct ttcccatctt
     2161 agtccaagag acaaattgta ttgcaaagcg ggtgtgtcag cacgctcgcc ctattagggc
     2221 tccgcttctc agcacgcctg ctccctccgg tcggcttgtc agcaatagag ctaatcaggg
     2281 aaagctggag cgtgccgatt aacctgctgt gtgaggaact tcaaggatct gaaggtttgg
     2341 gagagaagcc accgcctcgt cctggatgtc tatgatgcta ctggcagatt tcctagcgag
     2401 tagacgtttg ggcttaccgc caactgcgtc gttgctgtgc ctcgatccct accaacatag
     2461 ccgagggatg tgggaggacc ggtgaagctg agcttgcccg cttcatgctc atctcgatgg
     2521 gctcggccag cgagctcgag taccatttgc tcctcgcacg cgacctgggc taccttagcg
     2581 ccccccatta ccaggaactc ttgcaagaag cgggcgaggt caagcgaatg ctctcgacct
     2641 tcataaccaa gctgcgccac accaagtagc tgaaatgctg ac
//
LOCUS       DASTUV010000085         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_98329, whole genome shotgun sequence.
ACCESSION   DASTUV010000085 DASTUV010000000
VERSION     DASTUV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98329"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFT03_01815"
     CDS             complement(<1..660)
                     /locus_tag="VFT03_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846907.1"
                     /translation="MRLGYHRRRLADFAGGMRLSRELAEHEHWPEERLRQHQHERLQA
                     LVRHAVEHSPYYWERLSGVVGNDPVELSSLPVLDKAQMMEHYDELVTDSRLRRDELLA
                     WVEGLKRDELYLGEYRAMTTSGSSGRKGLFVYDEQGWRAIAAMFFRQSAWMGVKPRFP
                     HRFRMAIVGGAAPSHMTRQGSATLGVGIHRFLGTPLTLPVEQIVEKLNSFQPEYLNAY
                     PT"
     gene            complement(657..1232)
                     /locus_tag="VFT03_01820"
     CDS             complement(657..1232)
                     /locus_tag="VFT03_01820"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4846908.1"
                     /translation="MAAADALFTARGFHATSVDEIALEAGYTKGAVYSNFESKEDLFF
                     AIYERRATRVVAEYERTIREAGTVDGIERLIAQAARRRNSKEDGWLAVFFEFWAHVVR
                     RPELRERFAKIHARVLEPIVSGIERLMEEHGIQLPVETKQYTVAVYAMSLGLSLERLT
                     QPDVVDGTLGSRMARLLFEDLWRRDGKKEER"
     gene            1608..1952
                     /locus_tag="VFT03_01825"
     CDS             1608..1952
                     /locus_tag="VFT03_01825"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU4846909.1"
                     /translation="MSLYMTQFAYTAEAWKALVKEPEDRAAVFAQHAEKMGGRMVAYY
                     YCMGEYDGLVIFEAPDETTAAAIVFTVISPGHLKATRTTALMSVESTLEAMRRANAEA
                     FPGPKLWSPMPS"
     gene            2007..2990
                     /locus_tag="VFT03_01830"
     CDS             2007..2990
                     /locus_tag="VFT03_01830"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU4846910.1"
                     /translation="MFRTRLCDLLGIEHPVIQASIGPWSSAELVAAVSNAGGLGSVGT
                     ALQTAEDLRRQLSRVRELTDRPFAINHTARPFNEEAFVLSLQAGPKLVSFALGDPGDL
                     TKRAHDAGILFMQQIHTVGQAYRAAERGVDAIVAQGSEAGGFGGTVGAMALIPQVVDA
                     VSPIPVVAAGGIADGRGLAAALLLGAQGVNIGTRFLASTEATVPDDWKQRILAADSED
                     AIKVEFADHVFPRPSREGGYGTLPRVLRTPFVEEWNRRGDEVELEAERLGGELVAAIR
                     KGRAHELVPFTGQTAGQIHEVLPAGEIVRQMVAGAEEALRRANTSLRAQEN"
     gene            complement(3089..>3452)
                     /locus_tag="VFT03_01835"
     CDS             complement(3089..>3452)
                     /locus_tag="VFT03_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846911.1"
                     /translation="EPRALGLVEIRPREGAYNFEAKYTPGATDFAIPAEIPPEAAANL
                     EETALTTYRALGCSGYARVDTILADDTPWVIDVKTIPGFTETSTFPLAAEAAGISFES
                     LVETILEAALHTEATAKL"
BASE COUNT          625 a         1057 c         1168 g          602 t
ORIGIN      
        1 agtggggtag gcattcaagt attcgggctg gaagctgttg agtttctcca ctatctgctc
       61 gactggcagc gtgaggggtg tgcccaggaa gcggtggatg ccgacgccga gggtggcaga
      121 gccctgccgg gtcatgtgcg agggagccgc gcctcctacg atggccatcc tgaagcggtg
      181 cgggaaccta ggctttaccc ccatccacgc gctctggcgg aagaacatgg cggcgatggc
      241 ccgccagccc tgctcgtcgt agacaaagag acccttgcgc cccgaggagc cgctcgtcgt
      301 catcgcccga tactccccga ggtagagctc gtcgcgcttc aggccctcga cccacgccag
      361 cagctcgtcg cgccttagac ggctgtccgt caccagttcg tcgtagtgct ccatcatctg
      421 tgctttgtcg agtacgggca ggctcgaaag ctcgaccgga tcattcccca ccacgcccga
      481 aagccgctcc cagtaatacg gcgagtgttc gacggcgtgc ctcacgagcg cctggaggcg
      541 ctcatgctgg tgttggcgaa gcctctcttc gggccagtgt tcgtgttcgg ccagctcgcg
      601 cgagaggcgc atccctcccg cgaagtccgc caggcggcgc ctgtggtatc cgagcctcat
      661 cgctcctcct ttttgccatc gcggcgccag aggtcctcga acaacaggcg cgccatccgc
      721 gacccgagcg tcccgtcgac cacgtccggt tgcgtgaggc gctccagcga gagcccgagc
      781 gacatcgcgt acaccgccac ggtatattgc ttcgtctcga ccggaagctg gatgccgtgc
      841 tcctccatga gacgttcgat cccggagact atcggctcca gcacgcgagc gtggatcttc
      901 gcgaagcgct cgcgcaactc gggacggcgc accacgtgcg cccagaactc gaagaagacc
      961 gccagccagc cgtcttcctt gctgtttcgc cgccgcgccg cctgcgcgat gaggcgctcg
     1021 atcccatcga ccgtaccggc ctcgcgaatg gttcgctcgt actcggccac gacccgagtg
     1081 gcccgccgct cgtagatcgc gaagaagaga tcctctttgg actcgaagtt tgagtagacc
     1141 gcacccttgg tgtatccagc ctccagggcg atctcgtcca cagacgtcgc atgaaagccg
     1201 cgcgcggtga agagggcgtc agcagccgcc aacaagtcct ctcgcgtgcg ctcgcgtcgc
     1261 tcggctcggg tgagacgttt tcgcatacca atggtatctt aatactgctg gtatctgaat
     1321 gtcaagatcg gcgagttcgc acgaggtgct gggcacggtg agcgtagatc gtggactaag
     1381 aaggtccggg gaaagagacc gtcgttccga ggcggtcatg acggcgtgat cctgggcagt
     1441 aaagtgaacc ccgggaacgg ttgcgtgctc cttcaagggc tataatgtaa taaacgaaac
     1501 aatagcctgg gaggacagat ctcgaaaggt ggtgagctct atcgctctgg ggtcagggat
     1561 ctcgaacggc ataacacgtt gggtgcttga atgaggaggt caggggcatg tccctctaca
     1621 tgacgcagtt cgcctacact gcggaggcct ggaaggcgct cgtcaaggag cctgaggacc
     1681 gggctgcggt gttcgcccag catgcggaga agatgggtgg gcgcatggtc gcttactact
     1741 actgcatggg agaatacgac gggctggtca tcttcgaagc tccggacgag acaacggccg
     1801 cggccatcgt gttcaccgtg atctcgcccg gtcacctcaa ggcgaccagg acgaccgcgc
     1861 tgatgagcgt cgagagcacc ctggaggcga tgcgcagagc caacgccgag gcgttcccgg
     1921 gaccaaagct gtggtccccg atgcccagtt gagctggtca gcaaatcaaa tagttgctaa
     1981 ctggctgagt ttgagagggg gtaaacatgt tccgcacccg gctctgcgat ctcctgggca
     2041 tcgagcatcc ggtgatacag gcttccatcg ggccgtggtc ttcggccgag ctggtcgccg
     2101 ccgtctccaa cgccggaggg ctgggaagcg tcggaacagc ccttcagacg gcggaggact
     2161 tgcggaggca actgtctcgt gtgcgcgagc tgaccgatcg acccttcgcc ataaatcaca
     2221 cggcgcgacc gttcaacgaa gaggccttcg tcctgagcct gcaagccggg ccgaagctag
     2281 tctcgttcgc ccttggcgac ccgggcgacc ttaccaagcg tgctcacgat gctggcatcc
     2341 tcttcatgca gcagatccac accgtggggc aggcgtaccg ggcggccgag aggggcgtgg
     2401 acgcgatcgt cgcccagggc tccgaggccg gcgggttcgg gggcacggtg ggcgccatgg
     2461 ctctcatccc gcaggtcgtt gacgcggtca gccctatacc cgtggtggcg gccggtggca
     2521 tcgctgacgg gcgcgggctg gccgcggccc tgctgctcgg cgcccagggg gtcaatatcg
     2581 ggacccggtt cctggcttcg acggaagcaa cggtcccaga cgactggaag caaaggatcc
     2641 tggccgccga ttccgaggat gccatcaagg tcgagttcgc cgaccacgtc ttcccgcgac
     2701 cgagcaggga gggcggctac gggaccctgc cgcgggtgct gcgcacaccg ttcgtcgagg
     2761 agtggaaccg gagaggggac gaggtcgagc tggaagcgga gcggttgggc ggagagctgg
     2821 tggcagccat ccggaaaggc agggcgcacg aattagtgcc cttcaccgga cagactgcgg
     2881 gccagatcca cgaggtcttg cccgcgggag agatagtacg ccagatggtc gccggggcgg
     2941 aggaggccct gcgaagggca aacacctccc ttcgagctca ggaaaactga attgctgaca
     3001 agcgaggccg caaggccgag cgtgctgaca agccgaccga agggaggcgt gctgacaagc
     3061 gatgccttaa aagggcgagc gtgctaactc acagcttcgc ggttgcttcg gtgtggaggg
     3121 ctgcctccag gatcgtctcg accaggctct cgaaggagat accggcggcc tcggcggcga
     3181 gggggaaggt ggaggtctcc gtgaaaccgg ggatggtctt cacgtcgatg acccaggggg
     3241 tgtcgtctgc gaggatggta tccaccctgg cgtagccgga gcaaccgagc gcgcggtagg
     3301 tggttagcgc cgtctcctcg aggttggctg cggcctccgg ggggatctcg gccgggatcg
     3361 cgaagtccgt agcgcccggg gtgtacttgg cctcgaagtt gtaggcgccc tcgcgaggcc
     3421 ggatctcgac gagccccaga gcgcgtggct cc
//
LOCUS       DASTUV010000086         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1385548, whole genome shotgun sequence.
ACCESSION   DASTUV010000086 DASTUV010000000
VERSION     DASTUV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385548"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..267
                     /locus_tag="VFT03_01840"
     CDS             43..267
                     /locus_tag="VFT03_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846912.1"
                     /translation="MHGIERHREMSDPAGSQGGITKVVVRYADGRVLNFVADARRPAF
                     SEDDMLELKKVIDQAASAAEWAHINTRLGF"
     gene            complement(316..594)
                     /locus_tag="VFT03_01845"
     CDS             complement(316..594)
                     /locus_tag="VFT03_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEU4846913.1"
                     /translation="MEQAKRRMLGMAQGVMGRIAELTRERERLLAREAGHHATREDKE
                     RLSQIDHDLQVLWDLRRRELAGETVDLDEDYFDRYDIDPGDDAPDQYR"
     gene            663..1076
                     /gene="mce"
                     /locus_tag="VFT03_01850"
     CDS             663..1076
                     /gene="mce"
                     /locus_tag="VFT03_01850"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU4846914.1"
                     /translation="MLEKIHHLGYAVQSIEVASRFYEANFDVELSGPETVEGQGIVAT
                     MFRVGESAVELMEPTRPDSPVGKFLAKRGEGLHHVAFEVEDLEAALARLKSSGARLVD
                     EEPKVGMGGTRTAFVHPKGTFGVLTELVELPDKAR"
     gene            1093..2679
                     /locus_tag="VFT03_01855"
     CDS             1093..2679
                     /locus_tag="VFT03_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU4846915.1"
                     /translation="MDVDERRTESGIEVKPVYRPEDLGEGFDYAERLGDPGEYPYTRG
                     IYPSMYRGRPWTMRQYAGFGTAKETNARFKYLTANGQTGLSVAFDLPTQMGRDSDHEL
                     ALGEVGKVGVAIDSLLDMRDLFEGIPLREVSTSMTINATASILLLLYALVAEEQGVNK
                     EDITGTTQNDILKEYLARGTYIYPPEPSMRITTDTFAYCARELPRWNMISISGYHIRE
                     AGSTAVQELAFTLSNAIAYVEAALDAGLEVDEFAPRLSFFFNAHNDLFQEVAKYRAAR
                     RMWATIVKERFGATDERSLKLRFHTQTAGSSLTAQQAENNIVRTTVQALSAVMGGTQS
                     LHTNAFDEALALPTERSARVALRTQQILAHEGGLTGTADPLAGSYYVEALTDAVEEEA
                     WEYIRRIDALGGSVRAIEERYMQREIEDSAYRYQREIERGERTIVGVNRYTSEADDPD
                     MDLHTVDESIRERQSERLAELKETRDNAAVERALADLRAAAEGSDNLLYPTKEALANL
                     ATVGEVSDVLRGVFGQYRPV"
     gene            complement(2732..3661)
                     /gene="mdh"
                     /locus_tag="VFT03_01860"
     CDS             complement(2732..3661)
                     /gene="mdh"
                     /locus_tag="VFT03_01860"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU4846916.1"
                     /translation="MAKSKVTVVGAGNVGGTTAQRLAERNYADVVLVDIVEGLPQGKA
                     LDILESGPVIGFDSNVIGTNGYEESAGSDVVVITSGSPRKPGMSRDDLLKTNQNIVWS
                     VTEEVVKYSPETILVMVANPLDAMCHVAMEVSGFPRERVVGMAGILDTARYRTFIAQD
                     LRVSVRDVYALVLGGHGDTMVPLPSMATVGGVPITKLLPWERVEEIVDRARNGGAEIV
                     SLLQSGSAFYAPSAAATEMVDAILLDQKRILPCAAYLQGEYGIEDLFVGVPVKLGASG
                     VEEIIELDLQEDELDDLRNSAGAVRELVEVLHG"
     gene            3758..4333
                     /locus_tag="VFT03_01865"
     CDS             3758..4333
                     /locus_tag="VFT03_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEU4846917.1"
                     /translation="MDVIQAIETRQSMGRVKQDPVPGELVERILDSAVHAPNHRLTEP
                     WRFHVFTGKGRGELARVRADIARLQAEADGESEEMAAGRISRERKKAFRAPVVIAVIS
                     KAGRDEVETLENYAACAAAVQNMQLTAHALGLATMWRTGSVVYHDRMREFFGLEEGDT
                     MVAHLYVGYPDMGERPRRREAARARTTWHEG"
BASE COUNT          827 a         1488 c         1398 g          726 t
ORIGIN      
        1 gcccacagcg tccgccaggc tcagagggac taggggaaga gcttgcacgg catagagaga
       61 cacagggaga tgagcgaccc ggccggttcg cagggcggca taaccaaggt ggtcgtccgt
      121 tacgccgacg ggcgcgtcct caacttcgtg gccgacgccc gcagacccgc tttctccgag
      181 gacgacatgc tggagctcaa gaaggtgatc gaccaggccg cctcggcggc cgagtgggcg
      241 cacatcaaca cccggctggg cttctgagaa gaggggcgca tcgcgtaagg cgccttacgc
      301 gaacccctcc ttctcctacc ggtactggtc cggcgcgtcg tcgccggggt cgatgtcgta
      361 ccggtcgaag taatcctcgt cgaggtcaac ggtctccccg gccagctcgc ggcgtctcag
      421 gtcccacaag acctgcagat cgtggtctat ctggctgagc cgttccttat cctcgcgggt
      481 cgcgtggtgc ccggcttcgc gggcgagcag ccgctctcgc tctcgcgtga gctccgcgat
      541 ccttcccatc acgccctgcg ccatccccaa catcctcctc tttgcctgct ccatgaattc
      601 tagcggaatg tccgcttccc ctgctttggc tacaatctgg ttgccgccga cggagggaga
      661 gcatgctcga gaagatccac caccttggtt acgcggtaca gagcatagag gtcgcgtctc
      721 gcttctacga agcaaacttc gacgttgagc tcagcgggcc ggagacggtc gaaggacagg
      781 gcatagtagc cactatgttc cgggtcggcg agtctgcggt cgagttgatg gaacccacgc
      841 gccccgactc acccgtcggc aaattcctgg caaagcgagg cgaaggcttg caccacgtcg
      901 ctttcgaggt tgaagacctc gaggccgcac tggccaggct caagagcagc ggtgccaggc
      961 tcgtggacga ggagccgaag gtggggatgg gggggactcg gacggctttc gtccacccca
     1021 agggcacctt cggggtcctc accgagctag tagaattgcc ggacaaggcc cgatgagaga
     1081 ggtgtgcggg acatggacgt agacgaacgg cgcacggagt ccggcatcga ggtcaaaccc
     1141 gtctacaggc ccgaggacct gggagaaggt ttcgactacg cagagaggct cggggacccg
     1201 ggcgagtacc cctacacgag aggcatatac ccgagcatgt accgcggcag gccgtggacg
     1261 atgcgccagt acgcgggctt cgggaccgcg aaggagacca acgcccgctt caagtacctc
     1321 acggcgaacg gccagacagg cctctccgtc gccttcgacc tcccgacaca gatgggccgc
     1381 gactccgacc acgaactcgc cctcggcgag gtaggcaagg tcggagtagc catagactcc
     1441 ctgctcgaca tgcgggacct cttcgagggc atacccctgc gcgaggtctc gacctccatg
     1501 acgataaacg ccacagcctc tattcttctg ctcctctacg cgcttgtcgc cgaggaacag
     1561 ggcgtgaaca aagaggacat aacaggcacg acgcagaacg acatcctcaa ggagtacctg
     1621 gcgcgcggaa cctacatcta cccgcccgaa ccctcgatga ggatcacgac cgacaccttc
     1681 gcctactgcg cgcgcgagct tccgcggtgg aacatgatct cgatctccgg ctaccacatc
     1741 cgcgaggccg ggagcaccgc cgtccaggag ctcgccttca ccctctcgaa cgccatagcc
     1801 tacgtcgaag cggcgctcga tgccggactc gaagtggacg agttcgcccc ccgcctctcc
     1861 tttttcttca acgcccacaa cgacctgttt caagaagttg cgaagtatcg ggccgcgcgg
     1921 cgcatgtggg ctaccatcgt gaaggagcgc ttcggcgcga ccgacgagcg gagcctgaag
     1981 ctccgcttcc acacccagac agcaggctca tccctcaccg cccagcaggc cgagaacaac
     2041 atagtccgca cgacggtgca ggcgctatcg gcggttatgg gcggaaccca gagcctgcac
     2101 accaacgcct tcgatgaggc tctggcgctc ccgaccgaga ggagcgctcg cgtcgcgctc
     2161 cgcacccagc agatcctcgc tcacgagggt ggcctcacag gcacagccga ccctctggcc
     2221 ggctcctact acgtggaggc gctgaccgac gccgtcgaag aggaggcgtg ggagtacatc
     2281 cggcgcatag acgctctggg aggcagcgtc cgcgccatcg aagagcgcta catgcagcgc
     2341 gagatcgagg attccgccta ccgctaccag cgtgagatcg agaggggaga gcgcactatc
     2401 gtcggcgtca accgttacac gagcgaagcc gacgaccccg acatggacct gcacacggtg
     2461 gacgaatcca tccgggagcg gcagtccgag cggctggccg agctcaaaga gacgagggac
     2521 aacgccgccg tcgagcgcgc gctggccgac ctcagggccg cagccgaggg ctcggacaac
     2581 ctgctctacc cgacgaagga ggcgctcgcg aacctcgcga cggtgggcga ggtctccgac
     2641 gtactgcgcg gggtcttcgg gcagtacagg cccgtctgag gcccttggaa tagggtcggg
     2701 gttcgcgaac tccgacccta cccatcgcgc tttaaccgtg caacacttcg accagctcgc
     2761 gcaccgctcc ggcgctgttc ctgaggtcgt cgagctcgtc ctcctgcagg tcgagctcga
     2821 tgatctcctc gacgccggag gctccgagct tgacggggac gccgacgaac aggtcttcta
     2881 tgccgtactc gccctgcagg taggcggcgc aggggaggat gcgcttctga tcgagcagga
     2941 tggcgtccac catctcggtt gcggccgcgg acggcgcgta gaaggcgctg ccgctctgca
     3001 gcagacttac tatctccgcc ccgccgttgc gggcgcggtc cacgatctcc tctacgcgct
     3061 cccagggcag caacttggtg atcggcaccc cgccgacggt cgccatgctc ggcagaggga
     3121 ccatcgtgtc cccgtgtccg ccgaggacga gcgcgtagac gtcccgcacc gagactcgaa
     3181 gatcctgagc gataaacgtg cggtagcggg ccgtgtcgag gatgcccgcc atcccgacta
     3241 cccgctcgcg cgggaagccg cttacctcca tcgcgacgtg gcacatcgcg tcgagcgggt
     3301 tcgcgaccat caccaggatc gtctccggcg agtatttgac cacctcctcg gttacgctcc
     3361 agacgatgtt ctggttggtc ttgaggaggt cgtcgcggct catccccggc ttgcggggac
     3421 tcccgctggt tatcacgacc acgtccgagc ccgcgctctc ctcgtagccg ttggtgccga
     3481 tcacgttcga gtcgaagccg atcacgggtc cgctctccag gatgtccagc gccttgcctt
     3541 gcggcaggcc ctcgactatg tccacgagga ccacgtctgc gtagttgcgc tccgccagcc
     3601 tctgggccgt cgtcccgccg acgttgccgg cgcccacaac cgtaaccttg ctctttgcca
     3661 cgggaactcc ctcctgtgcg taccgcggtg tgccacgcca tgatatgtga attctcgccg
     3721 ttgtgccgcc tcgatgcccg ataaaataga gcgcgtgatg gatgtgatcc aggccataga
     3781 gacgcgccag agtatgggac gtgtgaagca ggaccctgtc ccgggagagc tcgtcgagcg
     3841 catcctcgat agcgccgtcc acgcgcccaa ccacaggctc accgagccgt ggcgcttcca
     3901 cgtctttacc gggaagggcc gcggcgagtt ggcccgcgta cgggcagata tcgcccgcct
     3961 ccaggccgaa gccgatgggg agagcgagga gatggcggcc ggcaggatca gccgcgaacg
     4021 caagaaagcc ttccgcgcac ccgtggtcat agccgtgatc tcgaaggctg gtcgcgacga
     4081 ggtagaaacg ctagagaact acgccgcgtg cgccgccgcc gtgcagaaca tgcaactcac
     4141 ggcccatgcc ctcggcctcg ctacgatgtg gcgtacgggc tccgtcgtct accacgaccg
     4201 catgcgcgag ttcttcggcc tggaagaggg cgacacgatg gtcgcccacc tctacgtcgg
     4261 ctaccccgac atgggtgaga ggccccgccg gcgcgaagcc gcgcgcgcga ggacaacctg
     4321 gcacgaaggc tgagggagcg tcgtcgggct ccggtgatac caccgtcaca ataacggctc
     4381 accgggaagt agctactggt agaggacttt gactctggcg tcagttttct accgggatc
//
LOCUS       DASTUV010000087         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1599631, whole genome shotgun sequence.
ACCESSION   DASTUV010000087 DASTUV010000000
VERSION     DASTUV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599631"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1725)
                     /locus_tag="VFT03_01870"
     CDS             complement(208..1725)
                     /locus_tag="VFT03_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU4846918.1"
                     /translation="MAITVRELVGTPDLRTRVLAGASGLDREVSWAHVCELPDPTEYL
                     AHGELLMTVGFTIPKGASAQEAYIERLAEVGLSGLLVAEQMYAPELSSGLTSAADRLS
                     LPVLLTAYSVPFTAIARTVAEANRDAEHQRLLQAVRVYETARLAVGSGSDLMAQLGDV
                     TGCDLFVLDSERGHPLLAKAPAAPEGVGEALIEEMARRSEPMPAVLRLRTGTSPAIAL
                     FVPASRPAAMVAVSRTAEPPDLSILRHVATLAALEVEKLTADYERRRRLGAELLAGLI
                     DSRIAADSATHLLAERGLTEEPRLLACCASDSGANEHSNLHLRLEDRGILHLLLRRTP
                     VLTALLPGTTDAIDAFREEIDPAFPVGLSDPIGTVSRVPDAYREAMWALQGAKASGKP
                     VAKYGEDAALSPFLPRSLSESRRIVEHLLGPILDYDAAHDSRLAASLKVFLSENRSWQ
                     RAAADLHVHKQTLVYRMRRVEELTGRRLDRTEDVAQLWFALQAAEALGSLGSDPR"
BASE COUNT          376 a          659 c          692 g          354 t
ORIGIN      
        1 cgaggatcac gtgtcagcaa ccgcaggtat tgacacgtgc ataagcgatg tcgtcacacg
       61 gtcaatgcct cgaacaaaga ccgcaaccgc cttcgggaaa ggcgaaaagc tgggtcagag
      121 cgccgggcct atttgctgca actcgctgag actgtgcggc cccatcttgg tcacgtggga
      181 ggaaaagtcc cggtccgtgg gtgcgtgcta ccgtggatcc gaccccaggc ttccgagggc
      241 ttctgctgcc tggagggcga accagagttg cgctacgtct tcagtacgat ccaaccgtcg
      301 cccggtcaat tcttcgacgc gacgcatcct gtagacgagg gtctgtttgt gcacgtggag
      361 atcggcggcc gcgcgttgcc aggagcggtt ctccgagagg aacaccttca gcgaggcggc
      421 gagtcgcgag tcgtgagcgg cgtcgtagtc gaggatgggt ccgaggaggt gctcgacgat
      481 gcggcgcgac tcgctgaggc tgcggggtaa gaacggcgag agggccgcat cctcgccgta
      541 cttggcgacg ggcttgccgg aggctttggc gccctgcaac gcccacatgg cctcccggta
      601 agcgtcgggc acacgagata ccgtgccgat gggatcgctc aagcccacgg ggaacgcagg
      661 atctatctcc tcacggaaag cgtcgatcgc atcggtcgtg ccgggcagca gcgccgtgag
      721 gacgggcgtt cgcctcagca ggagatgcaa gatcccacga tcctccaggc ggaggtggag
      781 gttggaatgc tcgttagccc cgctgtcact cgcgcagcag gccaggaggc gaggctcctc
      841 ggtcaagccc ctctccgcga gcaggtgcgt ggccgagtct gcggcgatgc ggctgtctat
      901 gaggcccgcg agcagctccg ctcccaggcg ccgcctgcgc tcgtagtccg ccgtcaactt
      961 ctcgacctcc agcgccgcga gagtcgcgac gtggcgcagg atggagaggt ccggcggttc
     1021 ggccgtcctg gataccgcta ccatggcggc cggacgagac gccggtacga acagggctat
     1081 ggctggactc gtcccggttc gcagacgtag gacagccggc atgggctccg acctccgggc
     1141 catctcctcg atcaaggcct caccgacgcc ttcgggagca gctggtgctt ttgccagcag
     1201 agggtgaccg cgctcggagt ctagaacgaa caggtcacag ccggtcacgt cgccgagctg
     1261 ggccatcaaa tcggaaccgc tgcccacggc caggcgcgcg gtctcgtaga cgcggacggc
     1321 ttgcaacagc cgttggtgtt ccgcatcccg gttcgcctcc gcaaccgtgc gcgcgatggc
     1381 ggtgaacggg acgctgtagg cggtgaggag taccggcagc gagaggcggt ccgcagccga
     1441 ggtcagcccg gaggatagct ccggcgcgta catctgctcc gcgaccagca acccgctcaa
     1501 ccctacttcc gcgaggcgtt cgatgtaggc ctcctgcgcg gaagccccct tcggaatggt
     1561 gaagccaacc gtcatcagca gctcgccgtg cgccaggtac tccgtagggt cgggtaactc
     1621 gcaaacgtgc gcccaactca cctcgcgatc cagccccgaa gcacccgcga gcacccgcgt
     1681 cctgaggtcc ggtgtgccta ccagctccct taccgtgatg gccacgaacc attcatacca
     1741 tagtacgaaa cctttgtgca aattcgtgca ttgggaggat tgctcgcctc ccgtacgccg
     1801 ctcagaatcc tgaagcaggt atagggtttg gaggtagtgg attggcggct ggatctgacg
     1861 gtgatatccg ctcgcgccga gtggctgctg cgatctcccg gcgcgcgagg gacctgtagg
     1921 agcagaatca tgctcgccct gccgctgatc gtaaaacgac gacctggcgt ttagccgcca
     1981 ggaccttgca ggagggtgtc gagagccttt aggggcagat aggaggcacg gccagtggtc
     2041 cgctacgaac ccgcaaactc gctcgagacc ccgcgcttct c
//
LOCUS       DASTUV010000088         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_776688, whole genome shotgun sequence.
ACCESSION   DASTUV010000088 DASTUV010000000
VERSION     DASTUV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776688"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /gene="purF"
                     /locus_tag="VFT03_01875"
     CDS             complement(<1..1301)
                     /gene="purF"
                     /locus_tag="VFT03_01875"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU4846919.1"
                     /translation="MSESLQDNDKPREACGVFGLYSRELEGELAQRTYFGLCALQHRG
                     QESAGIAVSDGERTTAVRDMGLVSQVFDEARLAALEAGHIALGHVRYSTTGSASWENA
                     QPEFHGRGELNVAVAHNGNLVNAVSLGRELAEEGFEFNSTSDTTFIAAAAVKELEQGR
                     SVGEAVQAAMRRLEGAYSVAMIFRDKLVAFRDPHGFRPLCIGEVEGGYAVSSETCGLD
                     IIGARFLREVAPGEVVVIDDSGLHSFRGESPRPALCVFEYVYFARPDSRFDGREVAGA
                     RQRMGELLAEEAPAEADVVIPVPDSGISAAVGYSRRSGIPYAEGLIKNRYVGRTFIQP
                     TDGMRQIGLRLKLNPLPSVIKGRRVVVIDDSIVRGNTSRKLVKLLFEAGAKEIHFRVS
                     SPPITGPCYYGIDMDTRDQLVGANYSVEEIRDNIGATTLAYL"
     gene            complement(1321..1686)
                     /locus_tag="VFT03_01880"
     CDS             complement(1321..1686)
                     /locus_tag="VFT03_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4846920.1"
                     /translation="MSVDKVNIEEKLALFDERWKPKIVGELNGQYVKLVKFIGEFVWH
                     HHDDEDELFLVVKGRFRMEFRERSVWIEEGEFIVVPRGVEHRPVAEEEAHVLLFEPAS
                     TLNTGNVRNDRTVAELDRI"
     gene            complement(1683..>2880)
                     /locus_tag="VFT03_01885"
     CDS             complement(1683..>2880)
                     /locus_tag="VFT03_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU4846921.1"
                     /translation="GELRVKSGGEVVGSVPAEHLADAPLYERDVVRPSYLDEVQKLDV
                     ADIPEPGDYNEVLLEMLAHPNLCSRRSIFEQYDHEVGTDTVVLPGADAAVMRIKGTKL
                     GYAMTTDGRGRHCYLDPRGGGGAIVAEAYRNLSCVGARPVAITNCLNFGSPEKREAYY
                     QLAECIEGMAEACEALGTPVVSGNVSLYNETEAGAVYPTPVVGMVGVFEDVSRRATPA
                     FKREGDVVVVLGGGPPDGSLLAGSDYLDVVYGKVAGRPPRPDLAAEKEASDLVRDMIR
                     SGLVDTAHDLSGGGEAVALAEMALAGRVGFEYEGHEIESIISAGSTAEILFGEAGARF
                     LVAVPEERWNDLQNALGGVAYDRVGITGGDRFKIGDLVDVSLQDLRKAYERDLFRAPG
                     GAEVTH"
BASE COUNT          467 a         1009 c          882 g          522 t
ORIGIN      
        1 aggtaggcga gcgtggtagc gccgatgttg tcccggatct cctccaccga gtagttcgcg
       61 ccgacgagct gatctcttgt atccatgtct atgccgtagt agcaggggcc tgtgatcggc
      121 ggtgaggaga cgcggaagtg gatctccttc gccccagcct cgaagaggag cttgacgagc
      181 ttgcggctcg tgttcccccg cactatggag tcgtctatga cgacgacgcg cctgcccttt
      241 atcacggagg gcagcgggtt gagcttgagc ctcaggccga tctggcgcat cccgtccgtc
      301 ggctggatga acgtccggcc gacgtagcgg ttctttatga gcccttcggc gtagggtatg
      361 ccgctccgcc tcgaataccc gacggcggcc gagatccccg agtccgggac cggtatcacg
      421 acgtcagcct cggccggagc ttcctcggcg agcagctccc ccatacgctg gcgcgcgccc
      481 gcgacctccc gcccgtcgaa gcgggagtcg ggacgggcga agtacacgta ctcgaagacg
      541 cagagcgccg ggcgcgggct ctctccacgg aagctgtgca gccccgaatc gtctatgacc
      601 acgacctcgc cgggagcgac ctcacgcagg aaccgggcgc ctatgatatc gaggccgcag
      661 gtctcgctcg agacggcgta gccgccttcg acctcgccta tgcacagcgg cctgaagccg
      721 tgggggtcgc ggaaggcgac gagcttgtcg cggaagatca tggcgacaga gtaggcgccc
      781 tccaggcgcc gcatggccgc ctgcacagcc tcgccgaccg aacggccctg ttccagctcc
      841 ttcacggccg cggcggctat aaaggtcgtg tcggaggtag agttgaactc aaagccctcc
      901 tccgcgagct cccgccccag gctcacggcg ttcaccaggt tgccgttgtg ggcgacggcg
      961 acgttgagct cgccgcggcc gtggaactcg ggctgggcgt tctcccacga cgcggagccg
     1021 gttgtcgagt accggacgtg acccagcgcg atgtgcccgg cctcgagcgc ggcgagcctc
     1081 gcctcgtcga agacctggga gacgaggccc atgtcgcgca cggccgtcgt cctctcgccg
     1141 tcggagacgg ctatcccggc tgactcctgg ccgcggtgct ggagggcgca gaggccgaag
     1201 tacgtgcgct gcgccagctc cccctccagc tcgcgcgagt acaggccgaa gacgccgcac
     1261 gcctctctgg gcttgtcgtt gtcctggaga ctctcgctca agaagctcac cctcccggtc
     1321 tcagatcctg tcaagctcgg cgaccgtccg atcgttcctg acgttgcccg tgtttagcgt
     1381 cgaggcgggc tcgaagagca gcacgtgcgc ctcctcctcg gccacggggc gatgctcgac
     1441 gccgcgcggg acgacgatga actctccctc ctcgatccag acgctccttt cccgaaactc
     1501 catccggaag cgccccttga cgacgaggaa tagttcatcc tcatcgtcgt ggtggtgcca
     1561 gacgaactcg ccgatgaact tgaccagctt gacgtactgc ccgttcagct cgcccactat
     1621 cttcggcttc caccgctcgt cgaagagcgc cagcttctcc tcgatgttca ctttatcgac
     1681 gctcaatgag tcacctccgc ccctccaggc gcgcggaaga ggtcccgctc gtatgctttc
     1741 ctgagatctt gcaaactcac gtctacaaga tcgccgatct tgaacctgtc cccgccggta
     1801 attccgaccc tgtcgtaagc cacaccgccc agggcgttct gcagatcgtt ccaccgctct
     1861 tccgggactg cgaccagaaa acgtgctccc gcttctccaa agaggatctc ggctgtactc
     1921 ccggcactga taatgctctc tatctcatgg ccttcgtact cgaacccgac acgaccagcc
     1981 agcgccatct ccgcgagcgc taccgcctcc ccgccgcccg acaggtcatg cgccgtgtca
     2041 acgagcccgg aacggatcat gtctcggacg agatcggaag cctctttctc cgccgcgaga
     2101 tccggcctcg gcggtctacc cgcgaccttg ccgtacacca catccaggta gtcggagcct
     2161 gcaaggagtg acccatcagg cggccctccc ccgagcacca ctactacgtc tccctcgcgc
     2221 ttgaaggcag gcgtggcgcg gcggctaacg tcctcgaaga cgccgaccat gccaacgacg
     2281 ggcgtcgggt agacggctcc ggcttcggtc tcgttgtaga ggcttacgtt gccgctcacg
     2341 accggggtgc cgagcgcttc gcacgcctcg gccatgcctt cgatgcactc ggcgagctga
     2401 tagtaggcct ctcgtttctc cgggctgccg aagttgaggc agttcgtgat ggcgaccggc
     2461 ctcgccccga cgcacgagag gttgcggtac gcctcggcta ctatcgcgcc gccgccgcct
     2521 ctcgggtcga gatagcagtg acggccccgg ccgtccgtgg tcatggcgta gccgagcttc
     2581 gtgcctttta tccgcatcac ggcggcgtcc gcccccggca acaccacggt atcggtgccc
     2641 acctcatgat catactgctc gaagatcgaa cgccgcgagc agaggttcgg gtgcgcgagc
     2701 atctctagca gcacctcgtt atagtccccc ggctcgggga tgtccgctac gtcgagcttt
     2761 tgtacctcgt cgaggtagga gggacggacc acgtcccgct cgtagagcgg ggcgtcggcg
     2821 aggtgttcgg cggggacgga gccgacgacc tcaccgccgc tcttcacgcg cagctccccg
//
LOCUS       DASTUV010000089         5525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1241566, whole genome shotgun sequence.
ACCESSION   DASTUV010000089 DASTUV010000000
VERSION     DASTUV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241566"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1023)
                     /gene="secF"
                     /locus_tag="VFT03_01890"
     CDS             complement(139..1023)
                     /gene="secF"
                     /locus_tag="VFT03_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU4846922.1"
                     /translation="MFSKVDFAGQWKVWFAISGVLLVAGIAAIALGNLNFGIDFEGGT
                     KFTVASPDHHLSEGEIRQALPGFAARDAVIQTAGNGYEVRTPVLSEEQRSQVEDSLGE
                     ALGGEVSVTTVSPTFGEQVRDQALQAVAAALLIIVAFISVRFEFAFALAAMVALLHDI
                     LMTVGFYAIVGREVTLVTVVAVLTVLGYSLYDTIIIFDRIRENTPRVGYNRRRFDQTI
                     NDSIRQVVRRSIYTSLCTLIPLTALLIFGETTLADFAFALLVGIIAGTYSSIFIASPV
                     LSLYKAWRAGRLRPARSA"
     gene            complement(1023..2489)
                     /gene="secD"
                     /locus_tag="VFT03_01895"
     CDS             complement(1023..2489)
                     /gene="secD"
                     /locus_tag="VFT03_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU4846923.1"
                     /translation="MGSQRNNLIILGMVVVLIGIAAYLIFIRQPVAQATQLGLDLEGG
                     VSVQMEGYQTDGSQVTHDEMEQAVGVIQQRVNSLGVTEPEIQIQGQNQVIVNIPGITD
                     ADRAVKVIGRTAQLGFYEVLATDGQLSVPQDEVDQVEKEIGQNLRDDDAYKEGKTKIL
                     FEVSPSPSGNGNFVAGYVVNEQPDMTGDAVQNANLDRDQAGRLEVTMDLTREGAGKFA
                     DLTASIAENALAAGQPGTGQLAIVLDKDVISAPTVEESITGGNVVIQNTSSPQGLPEQ
                     EAKELEIVLQTGALPVNMRILSVTAIGPTLGTESLKSGLLASLVGFGLVLLFLIAVYR
                     VLGVVADLALLIYGFLLWGLIVAIPVTMTLPGIAGIVLSIGVAADANIVIFERIKEEI
                     RRGKTARTAVKAGYEKGFRAILDGNITTLITAVILFALSSGSVRGFAVLLSIGVVLSM
                     FTAIVVTRALLGIVSSRGFHLSPAMMGVSKNDIGKKEG"
     gene            complement(2542..3741)
                     /gene="tgt"
                     /locus_tag="VFT03_01900"
     CDS             complement(2542..3741)
                     /gene="tgt"
                     /locus_tag="VFT03_01900"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU4846924.1"
                     /translation="MPGGGRGALPLLLVRGRDVDPERREAPIGAPVSIEVIARDGEAR
                     AGVLRTPHGEVETPTFMPVGTKGTVKGLTPGDLRTVGAGIVLGNTYHLYLRPGPDLVR
                     EAGGLHAFTGWDGPMLTDSGGYQVFSLARTRTITEKGVEFASVYDGSLHTFTPELVAR
                     VQEELGADIAMVLDECPPADAGREYHEESLRRTARWAARCKEAHGRRDQALFGIVQGG
                     LYPDLREESLARTLEIGFPGYAVGGLSVGEPREQMLETLSVTAPMLPADKPRYFMGIG
                     DPVGILEVIGLGVDMFDCVLPTRLARHGAALSAGGRVNLRNAGYRRDLGPLDPECSCE
                     TCAGYSRAYLAHLVREDELLGHRLITLHNVRFTVELCRLAREKIVAGGYEEWARAWIS
                     RYTEAAA"
     gene            complement(3657..4655)
                     /gene="queA"
                     /locus_tag="VFT03_01905"
     CDS             complement(3657..4655)
                     /gene="queA"
                     /locus_tag="VFT03_01905"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU4846925.1"
                     /translation="MLTSELDYELPEDLIAQRPASPRDASRLMVLDAEAGTISHHVFR
                     DLPRFLGPGDALVLNETKVLPARLLARRPGGGKVELLLLRDLGGPWEALVRPSKRLKP
                     GMALAVGGEELEVVKSLGEGRWMVAGDDLRATMERSGRMPLPPYIEATDEAEGAYQTV
                     YARHPGSAAAPTAGFHFTEEVLGETRRAGAEIARVTLHVGTGTFAPIRTERLEDHRMH
                     AEHYAVPDEAARTITDAGRVVAAGTTVARTLETWAASGEREGESDLFIHPGYEWRLVD
                     ALLTNFHLPRSTLLAMVMSFGGMELVREAYRVAVEERYRFYSFGDAMLILNGGRRR"
     gene            complement(4657..4875)
                     /locus_tag="VFT03_01910"
     CDS             complement(4657..4875)
                     /locus_tag="VFT03_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEU4846926.1"
                     /translation="MSIEGLGRILIGVAIVLLVLGGLFLLLGRLGLDRLPGDLVFRRG
                     NATVYFPIGLMILVSVVGTILLNLFFRR"
     gene            complement(4940..>5525)
                     /locus_tag="VFT03_01915"
     CDS             complement(4940..>5525)
                     /locus_tag="VFT03_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846927.1"
                     /translation="RRNWNWIKPAYVAVLVVSGIVVAPITVLPVLPVETLARITGAAG
                     GDAGVQVETREVGQLPQNFADRFGWENLVATVDRVYEELPAEERDRACILTGNYGEAG
                     AIDFFGPRHGLPGAISGHNSYYIWGPGDCTGEVVISVGVPRERLEEVFGEVEQADTVR
                     CRYCMPDEDNMPVYVCRDSKIPFKEAWPRFEHYD"
BASE COUNT          972 a         1870 c         1710 g          973 t
ORIGIN      
        1 cacatgatat cgcgaagaca ccaccagagt cgccggtctg caaggtatgc ttatcgctag
       61 actcccatga tgcagtgcca agatggggct cgaaggcggg gagggatcgg atgctcgtac
      121 agaatgcctc gaaaaggtct aggccgaccg cgcaggccgg agccttccgg cgcgccacgc
      181 cttgtagagc gacagtacgg gcgaggcgat gaagatggag ctgtaggtgc cggcgatgat
      241 gccgacgagg agggcgaagg cgaagtcggc cagcgtcgtc tcgccgaaga tcagtagcgc
      301 ggttaaggga atcagggtac acagcgaagt gtagatcgaa cgtctcacga cttgccggat
      361 agagtcgttg atcgtctggt cgaagcggcg gcggttgtaa ccgaccctcg gggtgttctc
      421 ccggatccga tcgaagatga tgatggtgtc gtagagcgag tatccgagca ccgtcaggac
      481 cgcgacaacc gtcaccaggg tgacctccct cccgactatg gcgtagaagc cgaccgtcat
      541 gaggatgtcg tgcaggagcg cgaccattgc ggccagcgcg aaggcgaact cgaagcgcac
      601 gcttatgaag gcgacgataa tgagcagcgc cgccgctacg gcctgcagag cctggtctct
      661 gacctgctcc ccgaaggtcg ggctcacggt ggtcacgctg acctcacccc ctagcgcctc
      721 tcccaaagag tcctcgacct gggacctctg ctcctcgctc agcacgggag tgcggacctc
      781 gtagccgttg ccagcggtct ggatcacggc gtcccgcgcc gcgaagccgg gcagcgcctg
      841 ccggatctcg ccctcgctca agtgatgatc cgggctcgcc acggtgaact tggtcccgcc
      901 ctcgaagtct atgccgaagt tcaggttccc gagggctatc gccgctatcc cggccacgag
      961 cagcacgccc gagatggcaa accacacctt ccactgcccg gcgaagtcga ccttggagaa
     1021 cactagcctt ctttcttccc gatatcgttc ttcgagacac ccatcatggc tggcgagaga
     1081 tggaagccgc ggctcgagac gatcccgagg agcgcgcgcg taaccactat cgcggtgaac
     1141 atgctcagga cgacaccgat gctcaggagg accgcgaagc ccctgaccga gccgctcgag
     1201 agggcgaaga ggatgacagc ggttatgagc gtcgtgatgt tgccgtccag aatagcccgg
     1261 aaacccttct cgtaaccggc cttgacggcg gtgcgggcgg tcttgccgcg ccggatctcc
     1321 tcttttatcc gctcgaatat gacgatgttg gcgtcggctg cgaccccgat actcagaacg
     1381 atccccgcga tcccaggtag cgtcatggtg accggaatag ccacgatgag cccccagagc
     1441 aagaacccgt agatgagcag cgcgaggtcg gccacgaccc cgagcaccct gtagaccgct
     1501 atcaggaaca gcagcacgag cccgaagccg acgagcgacg cgaggagccc gctcttcaat
     1561 gactccgtgc cgagcgtcgg accgatggcc gtcaccgaca ggatccgcat gttcaccggt
     1621 agcgcgccgg tctggagcac gatctcgagc tcttttgcct cttgctcggg tagcccttgc
     1681 ggcgagctcg tgttctggat gacgacgttg ccgccggtga tgctctcctc aaccgtcggg
     1741 gcgcttatca cgtctttgtc gagcactatg gcgagctgcc cggtccccgg ctgccccgca
     1801 gcgagcgcgt tctcggcgat actggccgtc aggtcagcga atttccctgc gccctcgcgc
     1861 gtgaggtcca tggtgacctc gagccggccc gcctggtcgc ggtcgaggtt cgcgttttgg
     1921 acggcatcac cggtcatgtc cggctgctcg ttcacgacgt acccggccac gaagtttcca
     1981 ttgcctgaag gcgaaggact cacttcgaag aggatcttgg tcttcccctc cttgtaagcg
     2041 tcgtcatctc gcagattctg gccgatctcc ttttcgacct gatcgacctc gtcctgcggg
     2101 acggagagct gcccatcagt ggccagaacc tcgtagaagc ctagctgggc ggtgcgtccg
     2161 atcaccttga ccgccctgtc ggcgtcggtg atgccgggga tgttcacgat gacctggttc
     2221 tggccctgga tctgtatctc gggctccgta accccgaggc tgttgacgcg ctgctggatg
     2281 accccgacgg cctgctccat ctcatcgtgg gtgacctggc tgccgtcggt ctggtaaccc
     2341 tccatctgca cgctgacccc accttcgagg tcgagcccca gctgggtggc ttgagccacc
     2401 ggctgcctga tgaagatcag gtaggccgcg atgccaatga gcaccactac cataccgagg
     2461 atgatcaggt tgtttctctg gctacccata cagtcggggc acaattctag cacccaatgg
     2521 tcacggcttg ctaaaaaatt gtcatgccgc ggcctcggta taccgggaga tccaggcgcg
     2581 cgcccactcc tcgtaaccgc cggccacgat cttctcccgc gccagcctgc acagctccac
     2641 cgtgaagcga acgttgtgga gggtgatgag acggtgcccc agcagctcat cttcccgcac
     2701 cagatgcgcc aggtaagccc tgctgtaccc ggcgcaggtc tcgcacgagc attccgggtc
     2761 gagcggccct aggtcccttc gataaccggc gttcctgagg ttgacgcgac ctccggcact
     2821 cagagcagcc ccgtggcggg cgagacgggt cgggagcacg cagtcgaaca tgtctacccc
     2881 gaggcctata acctcgagta tcccgaccgg gtccccgatg cccatgaagt accggggctt
     2941 gtcggccggt agcataggcg ctgtcacgga gagggtttcg agcatctgct cccgcggttc
     3001 tccgaccgag agcccgccga cggcgtagcc tgggaagcca atctcgaggg tgcgcgcgag
     3061 gctctcctcc cgcaggtcgg gatagagccc cccctgcacg atgccgaata gcgcctgatc
     3121 ccgcctccca tgcgcctcct tgcaacgcgc ggcccagcgc gccgtccgcc tcagggactc
     3181 ttcgtggtac tcacggccgg cgtcagcggg cgggcactcg tccagaacca tcgcgatgtc
     3241 agcgccaagc tcctcctgta ccctcgcgac gagctcgggc gtgaaggtgt ggagcgagcc
     3301 gtcgtagacg gaggcgaact cgacgccctt ctccgtaatg gttcgcgtcc tggccaggga
     3361 gaacacctgg tagccgcccg agtcggtgag catcgggcca tcccaccccg tgaacgcgtg
     3421 cagcccaccg gcctcgcgca cgagatccgg ccccggccgc aggtagaggt ggtaggtgtt
     3481 gccgagcacg atgcccgctc cgaccgtccg caaatcccct ggcgtaagcc ccttgaccgt
     3541 ccccttcgtc ccgacgggca tgaaggtcgg cgtctcgact tcgccgtgtg gggttcgcaa
     3601 taccccagcc cgcgcctcgc cgtcgcgcgc gatgacctct atggataccg gcgcccctat
     3661 cggcgcctcc cgccgttcag gatcaacatc gcgtccccga acgagtagaa gcggtagcgc
     3721 tcctcgaccg ccacccggta tgcctcccgc accagctcca tgccgccgaa gctcatgacc
     3781 atcgccagta gcgtcgagcg cgggaggtgg aagttcgtga gcagggcgtc gaccagccgc
     3841 cactcgtaac cgggatggat gaagaggtcg gattcaccct ctctctcgcc gctcgcagcc
     3901 cacgtctcga gcgtccgcgc cacggtagtc ccggccgcaa ctaccctacc ggcatccgtt
     3961 atcgttcgcg cggcctcgtc ggggacggcg tagtgctcgg cgtgcatccg gtggtcctcc
     4021 agcctctccg tccggatcgg ggcgaaggtc cccgtgccga cgtgcagcgt tacgcgcgcg
     4081 atctcggccc cagccctccg agtctccccg aggacttctt cggtgaagtg gaaccccgct
     4141 gtgggagccg ccgccgagcc ggggtggcgc gcgtagaccg tctggtaagc tccctccgcc
     4201 tcatccgtag cttcgatgta aggcggcaag ggcatccgcc cgctccgctc catagtcgct
     4261 cgcaggtcgt ctcccgctac catccagcgc ccctcgccga gactctttac gacttcgagt
     4321 tcttccccgc cgacggcgag cgccatccca ggcttcaaac gcttgctcgg gcggacgagc
     4381 gcctcccagg gaccgcccag gtcacgcaac aagagcagct cgaccttgcc gccgcccggc
     4441 ctgcgggcga gaagccgcgc cgggaggacc ttggtctcgt tcaggaccag ggcgtcgccg
     4501 ggccctagaa agcgcggcag gtcacggaag acgtggtgag atatggtgcc agcctccgcg
     4561 tccaggacca tcaggcgcga ggcgtcgcgc gggctggcgg gtcgctgggc gatcaggtct
     4621 tccggtagct cgtagtctag ctccgaggta agcaccctag cggcggaaga agaggttcag
     4681 caggatcgtg ccgactacgg agaccaggat catgagccct atcgggaagt agaccgttgc
     4741 attgccgcgc ctgaaaacga ggtcgcccgg cagccggtcg agtccgaggc ggccgagaag
     4801 caagaacaag ccgccgagca ccagcagcac gatcgccacg ccgatcagaa tcctgccaag
     4861 gccctcgatg ctcacattcc tattctattg gcggctcagg cgatgtccag catcggagac
     4921 cttacgactc gcactggtcc tagtcgtagt gctcgaagcg gggccacgcc tctttgaagg
     4981 gaatcttcga gtcccggcag acgtacactg gcatgttgtc ctcatccggc atgcaatacc
     5041 ggcacctgac agtatcggcc tgctcgactt ccccgaagac ctcttccagg cgttcccggg
     5101 ggacgccgac acttatgacg acctcgccgg tgcagtctcc cggaccccag atgtagtagc
     5161 tgttgtgacc gctgatcgcc ccggggagcc cgtgcctcgg cccgaagaag tcgatagcgc
     5221 ccgcctcccc gtaattgccg gtgaggatgc aggctctatc tctctcctcc gcgggcagct
     5281 cctcgtaaac ccggtccacg gtggcgacga ggttctccca gccgaacctg tcggcgaagt
     5341 tctggggaag ctgtccgacc tcgcgggtct cgacctgaac cccggcgtct cctccggcgg
     5401 cgcccgtgat cctcgccagc gtctcgacgg ggagtaccgg caggaccgtg atcggggcca
     5461 ctactatccc cgagaccacc agcacggcaa cgtaggccgg cttgatccag ttccagttgc
     5521 gtcgc
//
LOCUS       DASTUV010000090         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1027118, whole genome shotgun sequence.
ACCESSION   DASTUV010000090 DASTUV010000000
VERSION     DASTUV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027118"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFT03_01920"
     CDS             complement(<1..1067)
                     /locus_tag="VFT03_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU4846928.1"
                     /translation="MILLQKQTENQFYGPNLGYVLDLYERYREDPESVDERTREFFET
                     WSPPRANGRAASGAPVAADVEKDVGASKFIRHIRDFGHMAAQLDPLGSEPPGDPTLDP
                     GFYRISEEDLEDLPTLIIGGPVAERTSNAKEAADELRRLYCKSIGYDFGHVHAPDERF
                     WLREAVESERFGGTMDRSEARGLLRRLTQVDVLEKFLHKTFLGQKRFSIEGNDMVVPM
                     INYLVQAAANAGTPEVVMGMAHRGRLNVLAHVLDKPYSKVFGEFQQPERGEVSSAADS
                     PGEAWVGDVKYHLGIRGWQLSEEEADQEVLINLAPNPSHLEHVNPVVEGMARAAQEKR
                     DEAGEPRRDAEASLPILLHGDA"
     gene            complement(1335..3038)
                     /locus_tag="VFT03_01925"
     CDS             complement(1335..3038)
                     /locus_tag="VFT03_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4846929.1"
                     /translation="MSGGDSGASKPVLLVVEDNPEDLELIAHELLKRYGEDYHVVWES
                     STEAGMKRLRECEAAGEDVALVLADQWVPQMTGAEFLARARDVYPDTKRVLLVSQGDS
                     TIREPLLRSTALGEIDYYVVKPWRSPDEAFHRVITEFLDEWTGAHRPGFVALRVGGER
                     WSARSHEIRDLWSRSGVPLEFHAADSEKGKQLLARWDASPDRLPVVIIFDQRALVDPS
                     NAEVVDAFAQSIPFGVNIRPDQRNFDLVIVGAGPAGLAAAVYGASEGLDTLVVDAETF
                     GGQAGTSSLIRNYLGFSRGISGQELATQAYTQAWLFGASFDFARHATGLRRAEDGFLV
                     SLSDGIEVRGRSVLIATGVSYRRLGVPALEPLQGAGVFYSAAVTEAQATEGREVHVVG
                     GGNSAGQAAMHLSRYASKVMLLVRGASLAASMSEYLITEIEAAENIEVCLNTRVVDGK
                     GEGRLEHLVLEDSASGTVDTVSSAAVFVLIGGQPRTEWLSEDIVRDRGGYIVTGQDLL
                     LRDWGTPVVWTQERPPMLFETSVPGIFAAGDVRHGSVKRVASAVGEGSIAIPLIHRYL
                     N"
     gene            3217..3543
                     /locus_tag="VFT03_01930"
     CDS             3217..3543
                     /locus_tag="VFT03_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4846930.1"
                     /translation="MEGFENRIEYFERMVADNPDNPTGLLALANEYQKAGRYEDEAAA
                     LGRYLSVHSDDEGNAYARLGDVLTRLGRKDEARAAYETGIRQAEKHGHSGMADDLRLA
                     LIQLSE"
     gene            3801..>4134
                     /locus_tag="VFT03_01935"
     CDS             3801..>4134
                     /locus_tag="VFT03_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU4846931.1"
                     /translation="MKQPRRFETRAVHSGERRGGVDENGNFFPISTPIYASTTFSHHD
                     IETTDRVLGGEEPGYSYARYDNPTVVAFEEALASLEAPDGEARTFAFASGMSAMHAAM
                     TAAELGPGA"
BASE COUNT          748 a         1393 c         1247 g          746 t
ORIGIN      
        1 gcgtccccgt gcaggaggat cggcagcgac gcctccgcgt cgcgtcgcgg ctcgccggcc
       61 tcgtcgcgct tctcctgggc ggcgcgggcc atgccctcga ccacggggtt gacgtgctcg
      121 aggtggctcg gatttggcgc caggtttatg aggacctcct ggtcggcctc ctcctcgctc
      181 agctgccagc cccggatgcc gaggtggtac ttcacgtccc cgacccaggc ttcgccaggg
      241 ctgtcggcgg ccgatgaaac ctcgcccctc tccggctgct ggaactcgcc gaagaccttg
      301 gagtacggct tgtccagcac gtgggccagc acgttgaggc ggcccctatg cgccatcccc
      361 atgaccacct ctggagtccc cgcgttggcc gcggcctgca ccaggtagtt gatcatcggc
      421 actaccatgt cgttgccctc gatcgagaac cgcttctgcc cgaggaacgt cttgtggagg
      481 aacttctcta ggacgtcgac ctgcgttagc cgccgcagca gccctctggc ctcggacctg
      541 tccatggtgc cgccgaagcg ctcggactca accgcctcgc gcagccagaa ccgctcgtcg
      601 ggcgcgtgga cgtgcccgaa gtcgtagccg atcgacttgc agtacaggcg cctgagctcg
      661 tccgccgcct ccttcgcgtt ggaggtgcgt tccgcgacag gtccccctat tatgagggtg
      721 ggcaggtctt ccaggtcttc ctcgctgatg cggtagaagc cggggtcgag cgtgggatct
      781 ccgggcggct cgctcccgag cgggtccagc tgggccgcca tgtgcccgaa gtcgcgtatg
      841 tgccggatga acttggacgc cccgacgtcc ttctctacgt ccgccgccac aggcgcgccc
      901 gaggccgccc gcccgttggc ccgcggcggg ctccaggtct cgaagaactc ccgcgtacgt
      961 tcgtcgactg actccgggtc ttctctatac ctctcgtaga gatctaggac gtagccgagg
     1021 ttcggcccgt aaaactggtt ctcggtctgc ttctgcaaca ggatcacacc ctcacgtcaa
     1081 gtttaaactt ctgcaatacc aagcggcgca agaggaggcg cggccaacag atctcgcgcc
     1141 tccccgaggt gtcgtttttg ggggaagatt tacacatttt gatcctcttt atgatacccc
     1201 acatatccgc ttgcatctta ccctgaagtc gtgcccggca agcctgctaa ccgggactca
     1261 cgagcgcggc cctcacctaa tataaaacca cgcgcggtgg gaccgttaga tcgcgaataa
     1321 cgggttctca cgactcaatt caggtatctg tggataagcg gtatggcgat ggagccctca
     1381 cccacggcag atgccaccct ctttacggac ccatgcctca cgtctccggc agcgaagata
     1441 ccaggcacgc tggtctcaaa cagcatcggc ggacgttcct gtgtccacac tacgggtgtc
     1501 ccccagtcgc gcagcagcag gtcctgaccg gttacgatgt agcctcccct gtcgcgtacg
     1561 atgtcctcgg atagccactc ggtgcgaggc tggcctccta tgagcacgaa cacggctgca
     1621 gaggagactg tatccacagt accggaggcg gaatcctcga ggacgagatg ctccagccgt
     1681 ccctctcctt tgccatctac gactcgggtg ttgagacaga cctcgatatt ctctgccgct
     1741 tctatctctg ttatcaggta ctcggacata cttgccgcga gcgatgcgcc ccgcaccaac
     1801 aacatgacct tcgaggcgta cctggagagg tgcatagcgg cctgcccggc agagttcccc
     1861 cctcccacga catgcacctc ccggccctcg gtggcctggg cctcggtcac agcagcgctg
     1921 tagaatacgc ccgcgccctg caatggctcg agcgccggca ctcccaggcg gcggtaggag
     1981 acaccagtgg ctatcagcac actcctgcct cttacctcga tcccatcgga aagcgagacc
     2041 aggaacccat cctcggcgcg acgcagcccc gtcgcgtggc gcgcgaagtc gaaggaagcg
     2101 ccgaacagcc acgcctgggt gtaggcctgc gtagccagct cctggccgct tatcccgcgt
     2161 gagaagccga ggtagttgcg gatcagcgag ctggttcctg cctggccgcc gaaggtctct
     2221 gcgtccacaa ccagagtgtc gagaccctct gaggctccgt aaaccgcggc agcaagcccc
     2281 gcagggcctg ctcccacgat cacgaggtcg aagttccgct ggtccggcct tatgttgacc
     2341 ccgaacggga tgctctgcgc gaaggcatca accacctcgg cgttggaggg atcgacgagt
     2401 gcgcgttggt cgaaaatgat caccacggga agccgatccg ggctcgcgtc ccagcgggcg
     2461 agaagctgct tccccttctc ggagtctgct gcgtggaact caagcggcac cccgctgcgc
     2521 gaccacaggt ccctgatctc gtgcgaccgc gccgaccacc tctcgccgcc gacgcggagc
     2581 gcgacgaacc cggggcggtg tgcgccggtc cactcgtcca ggaactccgt tatgaccctg
     2641 tggaacgcct cgtcgggaga acgccacggc ttgacaacgt agtagtcgat ctccccgagc
     2701 gctgtcgacc gcagaagggg ctctcttatc gtgctgtcgc cctgcgatac gagcagcacc
     2761 cgcttggtgt ccgggtagac gtcgcgagcg cgggcgagga actcggctcc tgtcatttgc
     2821 ggcacccact ggtcggcgag cacgagtgcc acgtcctcgc ccgccgcctc gcattcccgc
     2881 aacctcttca tgcccgcctc tgtagagctt tcccacacga cgtggtagtc ctcgccatag
     2941 cgctttagaa gctcgtgtgc gatcagctct aggtcctcgg ggttgtcctc gacaaccaac
     3001 aacacgggct tggaagctcc cgaatctcca ccgctcatac cacgtccacc ttcctcacgc
     3061 acccggctgg tagaccgcga tagacaagct ccgagtttaa cgcatggact ccacgagaac
     3121 tcaactgtgg catacaaggg gcgtatacca atatcctcct acgctactcc actgacctat
     3181 actaaccctt tcgacgactg gtgagaggtg gttcagatgg agggcttcga gaacaggatc
     3241 gagtacttcg agcgaatggt cgcggacaat ccggataatc cgaccggctt gctagccctg
     3301 gccaacgaat accagaaggc cgggcgttac gaggacgagg cggcggcgct gggacgctac
     3361 ctctcagtcc acagcgacga cgagggcaac gcttacgcgc gcctgggcga cgtgctcaca
     3421 cgcctcgggc gcaaagacga ggcacgggcc gcatacgaga cgggcatccg gcaggccgag
     3481 aagcatggcc acagcggcat ggccgatgac ttgcggctcg cgctcataca gctgagcgag
     3541 tgagcacgct ccggctagcg ccgtcgcttg tcagcacgcc tcccttcggt cggcttctca
     3601 gctaggatag cgcggtccgt cgctccgtag cgctagccag agctactgcc gcaaagccgt
     3661 gtcgtaatgc gtgaccggga aagagcccag cggcagagag taagctgaaa tgctgagaag
     3721 cgagggccta aaagggccga gcgtgctgag aaggcgtctg gcgaagccgg agcgtgctgc
     3781 attgcgttag tatagtcgcc atgaagcagc cgagacgttt cgagacccgc gccgtccact
     3841 ccggcgagcg cagggggggc gtggacgaga acgggaactt cttccctatc tcgaccccga
     3901 tctacgcctc gacaaccttc tcccaccacg acatagagac tacggacagg gtcctcggcg
     3961 gcgaggagcc tggctacagc tacgcccgct acgacaaccc gacggtagtc gccttcgagg
     4021 aggcgctggc ctcgctcgag gctcctgacg gcgaagccag gactttcgcg ttcgcctcgg
     4081 ggatgtccgc gatgcacgcc gctatgacgg cggcggagct tggcccggga gcca
//
LOCUS       DASTUV010000091         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_604211, whole genome shotgun sequence.
ACCESSION   DASTUV010000091 DASTUV010000000
VERSION     DASTUV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604211"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFT03_01940"
     CDS             <1..518
                     /locus_tag="VFT03_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4846932.1"
                     /translation="RQTVDMVYEKIGREAPESRTSKVPLPGGETDDFEAFAARFKATS
                     GLTDELSERLLKLYGVRALDVLEIAGEDSSLRMPLGPSARVESGIIGAEVLYALRREL
                     AQTLSDVLLRRTMVGLGPNVGLDVDEAAAQVAVKHEGWSEERARNETEAYREYVERYK
                     PKVMRDEAAKV"
     gene            633..1142
                     /locus_tag="VFT03_01945"
     CDS             633..1142
                     /locus_tag="VFT03_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU4846933.1"
                     /translation="METNRSSEGILSRELTGKRGEIIELLKTAYFMELETVMSYVTNS
                     VNPDGVRAQEIKESLEEDIQEELGHAQQFAARIKELYGVVPGSMEFKATQPFLQPPEE
                     QTDIVHVIKGVIEAETGAIEHYTRIVEETDEVDLVTQDMVIDILRDEQGHRRLFEGFL
                     REYEAEGLA"
     gene            1323..2651
                     /locus_tag="VFT03_01950"
     CDS             1323..2651
                     /locus_tag="VFT03_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU4846934.1"
                     /translation="MEDPFLSWLNLFLALALIALNGFFVAAEFALVRVRDSRIQQLEQ
                     EGSARAGVVRDALRDLDDYLSVCQVGITVASLGLGWVGEPAVAHLIDPLLHAVGITSE
                     RAVTFISFVVGFALITYAHLVFGEQAPKYFSIQKAERTALWISRPLKIFMVLFRPLVW
                     LVNASTNFVLRPWGIKLGEEIEAHSEEELRIMISSSAASGELEPEERDYLNNVFEFGD
                     RVAREIMVPRPDIEALSVDMPLPEMVDAAVFGRYTRYPVYEGDLDHVIGAVHVKDLLR
                     TARENPDDFDIRTIVRDCLVVPENKPILQILKEFQTRKLQMAIVIDEWGSVEGLITIE
                     DIIEELVGEIQDEFDEGEAQIEKLGEGVYAVDGRIPITEVNEYFDLDLPHEDFDTIGG
                     YILGVLGRPPEAGDTVEVDGVILQVKSVDGPRVSMLTLQRKEDARPRPSD"
BASE COUNT          564 a          881 c          960 g          499 t
ORIGIN      
        1 cccgccagac ggtggatatg gtctacgaga agatcggccg cgaggcgccg gaaagccgca
       61 cctcgaaggt gcctttgccg gggggagaga cggatgactt cgaggccttc gccgcgcgct
      121 tcaaggcgac gagtggcctg actgacgagc tctccgagag gctcctcaag ctctacggcg
      181 tccgcgccct ggacgtgctc gagatagccg gcgaagactc gtcgctacgg atgcctctag
      241 gtccctccgc tagggtcgag agcggcatca tcggcgccga ggtgctctac gcgctcaggc
      301 gcgagctggc ccagaccctc tccgacgtgc tgctcaggcg tacgatggtc gggcttggcc
      361 cgaacgtcgg cctggacgta gacgaggccg ccgcgcaggt cgccgtaaag cacgagggtt
      421 ggagcgagga gcgcgccaga aacgagaccg aggcgtaccg tgagtacgtc gagcgctaca
      481 agccgaaggt catgcgggac gaagccgcga aggtctgaag cgcgggcatg ctgtaacgac
      541 tcccgctgta ctccacttca cgtttagcgg tggatggcga gggtacattt ctaggcataa
      601 cgtcgatgag accggagtag gaggtccgca gcgtggagac caaccgcagc agcgagggga
      661 tcctctcgcg ggagttgacc gggaagcgcg gggagatcat cgagctcctc aagaccgcgt
      721 acttcatgga gctcgagacg gtgatgagct acgtcaccaa ctccgtaaac cccgacggcg
      781 ttcgcgccca ggagatcaag gagtccctcg aagaggacat ccaggaagag ctcgggcacg
      841 cgcagcagtt cgccgcgcgc atcaaggagc tctacggggt ggtgccgggc tcgatggagt
      901 tcaaggctac ccagcccttc ctgcagcctc ccgaggagca gactgacatc gtgcacgtca
      961 tcaaaggcgt catagaggcc gagacgggtg ccatagagca ctacacccgg atcgtagagg
     1021 agaccgatga ggtcgacctg gtcacgcagg acatggtgat agacatcctg cgcgacgagc
     1081 agggccaccg gcgcctgttc gagggcttct tgcgcgagta cgaggccgaa gggctggcct
     1141 gaatagagca aagtagagat cgaggggccc ggcgcgccgg gccctttttc gtgcgcagga
     1201 gcgcgagttt gtgctaacga gtggctgggg tataatgacg ctcgtaacgt gatggacgaa
     1261 cctcttttga ccgaaccccg acctacgcag ccacgctgcg ttgccggtgc gggtgtatag
     1321 agttggaaga ccccttctta tcctggctta acctcttcct ggccctggcg ctgatcgctc
     1381 tcaacggctt cttcgtggcg gccgagttcg ccctcgtgcg ggtgcgggac tcgcgcatcc
     1441 agcaactcga gcaggagggc agcgccaggg ccggagtggt gcgcgacgcg ctgagggacc
     1501 tcgacgacta cctctccgtg tgccaggtcg ggatcacggt ggcctcgctg ggactgggct
     1561 gggtaggcga gccggcggta gctcacctca tcgatccgct tctccacgcg gtggggatca
     1621 ccagcgagag ggccgtcacc ttcatctcct tcgtcgtggg attcgcgctc attacatacg
     1681 ctcatctggt cttcggggag caggcgccca agtatttttc gatccagaag gccgagagga
     1741 cggcgctctg gatcagccgc ccgctcaaaa ttttcatggt cctgttcagg ccgctcgtgt
     1801 ggctcgtgaa cgcatctacc aacttcgtcc tgcggccctg gggtatcaag ctcggcgaag
     1861 agatagaagc tcactccgag gaagagctca ggatcatgat ctcctcctcg gccgcctcgg
     1921 gcgagctcga gccggaagaa cgcgactacc tcaacaacgt cttcgaattc ggggacaggg
     1981 tggcgcgaga gatcatggtc ccgaggcccg acatcgaggc cctgagcgtg gacatgcccc
     2041 tccccgagat ggtagacgcg gcggtcttcg gccgttacac tcgctaccct gtctacgagg
     2101 gggacctcga ccacgtcatc ggcgccgtcc acgtgaagga ccttttgcgc acagcgcgcg
     2161 agaacccgga cgacttcgac atcaggacga tagtccggga ttgcctcgtc gtccccgaga
     2221 acaagcccat tttgcagatc ttgaaggagt tccagacacg caagctgcag atggcgatcg
     2281 tgatagacga gtggggcagc gtcgaggggc tcatcacgat agaagacatc atcgaggaac
     2341 tcgtcgggga gatacaggac gagttcgatg aaggggaggc ccagatcgag aagcttgggg
     2401 agggtgtcta cgccgtagac ggccgcatcc ctattaccga agtgaacgag tacttcgacc
     2461 tcgacctccc gcacgaggac ttcgacacca tcggtggcta catcctgggc gtcctgggcc
     2521 gcccacccga agctggcgac accgtcgagg tcgacggcgt catcctccag gtgaagagcg
     2581 tggacggccc gcgcgtctcg atgcttaccc tccagcgcaa ggaagacgcc cgcccgcggc
     2641 cttctgactg atacccgggt gcgctctacg acctcgcctt gaccttcgag ccgtggaagt
     2701 tgaacggctc cgcctgagct ggtccggcgc ggcaacctcc gctacctcct gctaacattt
     2761 cacggtcgtg atcgcgcacc agatcggcgg acgcgtcctt gaccgaaacc gagagaaaac
     2821 acggcatcga gaccttgact agcggggcga tggccggggt ctgggcgtgg gtccgcgaca
     2881 ggctgccgca ggggggtggc gtct
//
LOCUS       DASTUV010000092         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_812451, whole genome shotgun sequence.
ACCESSION   DASTUV010000092 DASTUV010000000
VERSION     DASTUV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812451"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..466
                     /gene="hypA"
                     /locus_tag="VFT03_01955"
     CDS             98..466
                     /gene="hypA"
                     /locus_tag="VFT03_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEU4846935.1"
                     /translation="MHELAIAGSVVRIAERHADGRRVTKVSLKVGHLRQVVSSALAFS
                     FELVAQETAVEGAELVLEEVPATGKCCACGSESRLESFPLQCKACGGFDLKIVAGEEL
                     YVESLEIEDRTETQRLMPEA"
     gene            495..1172
                     /gene="hypB"
                     /locus_tag="VFT03_01960"
     CDS             495..1172
                     /gene="hypB"
                     /locus_tag="VFT03_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEU4846936.1"
                     /translation="MHRTAVKKVVFEGVLDANDALAQANREKFDQAGTYVINMMSSPG
                     AGKTALLEHTLERMRGKLRLGVLEGDVQTTLDADRLARFHVPLVQVNTDPGFGGECHL
                     DANMVRSGLAELPLDEMDVLIIENVGNLVCPAEFRVGEDARVMVYSITEGEEKPLKYP
                     LMFRSADLVLVNKMDLLEYLDFDLEQFLGNLDAVNPGVESLLTSARTGRGVDEWCTWL
                     ERRGAPS"
     gene            1246..2001
                     /locus_tag="VFT03_01965"
     CDS             1246..2001
                     /locus_tag="VFT03_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4846937.1"
                     /translation="MAAIDPWLEGFLQGSAGMGFILLVSLLLGLRHASDPDHLAAVTT
                     LIAAEEPEAVRKATFMGLSWGLGHGTTLVLLGLPLVLLGQYLPEEIQKSAEVAIGIII
                     VVLAVRLLVRWRRGVFHAHSHTHGSGDTHRHLHSHAEDSVHEHSHDTALRTPLSAYGV
                     GLVHGVGGSGGLTLLLLSTIPSPSEAVVALLIFAAGTASSMALLSTVFGRAIASGPVA
                     RNFERIAPVLGVLAAAFGVWYALGALGIVAYPF"
BASE COUNT          373 a          698 c          728 g          370 t
ORIGIN      
        1 ctcggactac atcccgcgca tccaggaggt acaggcctcc gtctaccacg tgctgcgcgg
       61 tctcatagag acgttgatgg agcagagagg ttagcgtgtg cacgagctcg ccatagccgg
      121 gtcggtggtc aggatcgccg agcgccacgc cgacggacgc cgggtcacga aagtgagtct
      181 gaaggtcggc cacctgcgac aggtcgtctc ctccgccctc gccttcagct tcgagctggt
      241 ggcacaggaa accgcagtcg aaggcgcgga gctggtgctg gaagaggtcc cggcgaccgg
      301 aaagtgctgc gcctgcggat cggagagtcg actggagagc ttccccctgc aatgcaaagc
      361 gtgcggaggc ttcgacctga agatcgtcgc gggagaagag ctgtacgtag agtcgctgga
      421 gatagaagat cggacagaga cccagaggct gatgcccgaa gcctgaaacc gcacaccgaa
      481 gggaggctcc acccatgcac cgcacggccg taaaaaaagt ggtcttcgag ggagtcctcg
      541 acgccaacga cgcgctcgcc caggccaaca gggagaagtt cgaccaggcc ggcacatacg
      601 tcataaacat gatgagctcc cccggggcgg ggaagacggc cttgctggag cacacgctgg
      661 aacgcatgcg cggcaaactc cggctgggcg tccttgaggg cgacgtgcag acgacgctcg
      721 acgccgacag gctcgcccgc ttccacgtcc cgctggtgca ggtgaacacg gaccccggct
      781 tcggcggcga gtgccacctc gacgcgaaca tggtgcgctc ggggctcgcc gagcttccgc
      841 tagacgagat ggacgtcctc atcatagaga acgtcgggaa cctggtgtgt ccggcggagt
      901 tcagagtcgg ggaggacgcg agggtgatgg tctactcgat caccgagggc gaggagaagc
      961 ccctcaagta ccctctgatg ttccgctcgg ccgatctggt gctggtgaac aagatggacc
     1021 tactggagta cctcgacttc gacctggagc agttcctcgg caacctggac gcggtcaacc
     1081 ccggcgtcga gagcttgctc acgagcgccc gcacgggtcg gggcgtggat gagtggtgca
     1141 cctggcttga gcgccgcggg gctccttcgt agttgacagc agcacgccgg ggtccctatt
     1201 gttgcgagga gcaggaggcg aggaccccga gaggaggagt tgcgcgtggc cgccatagat
     1261 ccctggctcg aagggttcct gcaaggctcc gcggggatgg gcttcatcct gctggtcagc
     1321 ctgcttctgg ggctgaggca cgccagcgac cctgaccacc tggccgccgt caccaccctc
     1381 atagccgccg aggagccgga ggctgtcagg aaagccacct tcatgggcct cagctggggc
     1441 ctcggccacg ggaccacgct agtcctcctg gggcttccgc tcgtgctgct gggccagtac
     1501 cttccggagg agatccagaa gtccgccgag gtggccatag ggatcatcat cgtggtgctc
     1561 gcggtccgac tgctggtgag gtggcgccgc ggcgtcttcc acgcccactc ccacacccac
     1621 gggagcggcg acacgcaccg acacctccac tcgcacgcgg aggattccgt gcacgagcac
     1681 tcccacgaca cggcgctccg cacgccgctc tcggcgtacg gcgtggggct cgtgcacggc
     1741 gtcggaggct ccggagggct cacgctgcta ctgctctcga ccatcccaag cccctcggag
     1801 gccgtcgtgg cgctactcat cttcgcggcc ggtaccgcca gctcgatggc cctgctctcg
     1861 accgtcttcg ggcgtgccat agcctctggc ccggtcgcca ggaacttcga gcgcatcgct
     1921 cccgtgctcg gggtgctggc cgccgccttc ggggtctggt acgccctcgg cgcccttggt
     1981 atcgttgcct acccgttcta aggcgcattt cagcctaatc tgttttgggg ctctcctggt
     2041 ttcttggcag gatcgatcat tcccagaagg ggcgtagtgc gctttgagtt gagggggttc
     2101 gcgaggaccg ccccttcgca atgattaccc ggcgcgttcg gcctgacgcg agatctccgc
     2161 ggcgtgtcg
//
LOCUS       DASTUV010000093         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1526214, whole genome shotgun sequence.
ACCESSION   DASTUV010000093 DASTUV010000000
VERSION     DASTUV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526214"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFT03_01970"
     CDS             <1..483
                     /locus_tag="VFT03_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846938.1"
                     /translation="FGDPFLFYTEQSRWGREATDPLDSLLSALSWGGESLRLLFSPRL
                     WADPSLGRVADALEAAGNAYNLVFLGLALALLVAGLRVLPPDLSAYAFLLILPPTLFG
                     AAQIPLMGLPRYVLVAFPLFIVLGVLLEGRRLLGLWLTLSAVSSLLLCALFVTWRFVA
                     "
     gene            complement(643..1845)
                     /locus_tag="VFT03_01975"
     CDS             complement(643..1845)
                     /locus_tag="VFT03_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4846939.1"
                     /translation="MKVLVLGGDGYCGWPTSLHLSDAGHEVVIVDNFVRRTIDHELAA
                     ESLTPIADPKGRVNAWREVSGQTIGLEYGDLLDWEFVSEIFTRHQPDAVVHFAEQRAA
                     PYSMIDRRHAVYTQHNNVIGNLNVLFAINEYVPDCHLVKLGTMGEYGQPNIDIEEGYI
                     EIEHNGRKDVLPFPKQPGSWYHLSKVHDSHNIHFACKIWGLRATDLNQGVVYGTVTPQ
                     TERDLRLLNRFDYEEVFGTVLNRFCAQAAIDHPLTVYGNGSQTRGYLDIRDTVRCIEL
                     AVDNPAERGEMRVFNQFTEEFSVRELAEMVVESASKLGISARIENIENPRVEKYDHYY
                     NATHTGLLDLGLEPHYLGDSLLDSLLNIAIEYRDRVDTDHVMPSVDWRTGRSKERVTK
                     APAEPSAL"
     gene            complement(2004..>3423)
                     /locus_tag="VFT03_01980"
     CDS             complement(2004..>3423)
                     /locus_tag="VFT03_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU4846940.1"
                     /translation="ITDRLPNLPGRPSGEGSNVVLVITDSLRKDHIGAYGNGWIQTPN
                     LDALAKESLRFTRAYPESAPTICARRAIHTGLRTWPFRDWKPPKGEDIILQGWQPIPE
                     GQTHLAEIMKQNGYATLLQTDNMHQYKASYNFQQGFDVFDFIRGQTTDNYMPNWTFPH
                     DRVQNALLKGNVSAMRAQMQQYWANVEGRLVAERESEEDWFSPRVFSGAMELLDAAKG
                     GDQPFFLVADSYDPHEPWDTPDEYVNLYDDPYDGREPYSVIYGSSDYLTERELQRMHA
                     RYAAEVTMVDRWFGRFLDRMDELDLFENTLLLVIADHGVAFGEHGYTGKPSYVLWPEI
                     TDVPFMIRHPEGKGAGETSDYYASTHDVAPTILGFLGIEPEQPMEGQDLSVLFDGGQP
                     EERPYFTLGYNDHAWARDDRYVMFAKNDGSNALLYDVSTDPEMNKDIAGPNQGIINSM
                     WNDYILEDAGGPLPTYRNARPY"
BASE COUNT          538 a         1075 c         1092 g          718 t
ORIGIN      
        1 ttcggagatc ccttcctgtt ctacaccgag cagagccgct ggggccggga agcaacagac
       61 ccactggact cgctcctgag cgccctctcg tggggcgggg agagcctcag actgctcttc
      121 tccccgcgcc tctgggccga cccttccctc gggcgcgtcg cggacgcgct ggaagctgct
      181 ggcaacgcct acaacctggt cttcctgggc ctcgcgctgg cgctgctcgt cgcgggcctg
      241 cgggtgctgc cccccgacct cagcgcctac gccttcctgc tcatcctgcc ccccaccctc
      301 ttcggggcgg cgcagatccc gctgatgggg ctgccgcgct acgtgctcgt ggccttcccc
      361 ctgttcatcg tgctcggcgt gctgctcgaa gggcgccggc tcctcggcct ctggctgacc
      421 ctgagcgccg tctcctccct cctcctctgc gccctcttcg tcacctggag attcgtggcg
      481 tagcaggggc ggttcgcgaa ccgcccttac accaaccacc cgtacacaat ttcgctacgc
      541 gccggtggct cgatcacgtg ttgggggtct cgcagcgaag cgacgctccc ggaagggagc
      601 gccgccataa tagacctata cctgatgcct acggcttgaa acctacaacg cggacggctc
      661 tgcgggtgcc ttggtgacgc gctccttcga gcgtcccgtg cgccagtcta cgctcggcat
      721 gacgtggtcg gtgtccacgc ggtcgcggta ctcgatggcg atgttcagga gcgagtcgag
      781 gaggctgtcg cccaggtagt ggggctcgag gccgagatcg agcaggccag tgtgcgtagc
      841 attgtagtaa tggtcgtact tctcgacgcg cgggttctcg atgttctcta tccttgcaga
      901 gatgccgagc ttggaggcgc tctcgacgac catctcggcg agctccctga ccgagaactc
      961 ctcggtgaac tggttgaaga cccgcatctc gccgcgctcg gcggggttat cgacggcgag
     1021 ctcgatgcaa cgcaccgtgt ctcggatgtc caggtagccc cttgtctggg agccgttgcc
     1081 gtagaccgtg agcgggtgat cgatcgcagc ttgcgcgcag aagcggttca ggacggtccc
     1141 gaagacctcc tcgtaatcga agcgattcag caggcgcaga tcacgctcgg tctgcggcgt
     1201 gaccgtgccg tagactacac cctggttcag atcggtggcc ctaaggcccc atatcttgca
     1261 ggcgaagtga atgttgtgcg agtcgtggac cttcgagagg tggtaccagc taccgggctg
     1321 ctttgggaaa ggcagaacgt ccttgcggcc gttgtgctcg atctcgatgt agccctcttc
     1381 gatgtcgatg ttgggctgcc cgtactcacc catcgtcccg agcttgacga ggtggcaatc
     1441 cggcacgtac tcgttaatcg cgaagagcac gttgaggttg ccgatcacgt tgttgtgctg
     1501 cgtgtagacg gcgtgacgcc ggtctatcat cgagtagggc gccgcccgct gctcggcgaa
     1561 gtgtacgacc gcgtccggct ggtggcgcgt gaagatctcc gagacgaact cccagtccag
     1621 caggtcacca tactccaacc cgatcgtctg gccgctgacc tcccgccacg cgttcacacg
     1681 ccccttgggg tcggctatcg gcgtgaggct ctctgcagcc agctcgtggt ctatcgtcct
     1741 gcggacgaag ttgtccacga tcacgacctc gtgacccgcg tcagagagat gcagggaggt
     1801 cggccagcca cagtagccgt cgccccccag aaccaacacc ttcatcgctc gcgcctcctt
     1861 ctcgatactg cctggtttct catcaaaact acaccaaact taaaccggca tgctggccga
     1921 cgattgtagc agagaagctg tggaatgatt atccaacgct acaagcgccg atacaagcgg
     1981 tggcaccagc cggagtgtgt tctctagtag ggacgtgcgt tcctgtaggt tggcaggggc
     2041 ccgcccgcgt cctcgaggat gtagtcgttc cacatgctgt tgatgatgcc ctggtttggc
     2101 cccgctatgt ccttgttcat ctctggatca gtactgacat cgtagagcag ggcgttggac
     2161 ccgtcgttct tggcgaacat gacgtagcgg tcgtcgcgcg cccaggcatg gtcgttgtag
     2221 cctagagtaa agtacggccg ctcctcgggc tgcccaccat cgaagagcac ggagaggtcc
     2281 tggccttcca tgggttgctc gggctctatg ccgaggaagc ccaggatggt tggggccacg
     2341 tcgtgggtgg aggcgtagta gtcgctggtc tctccggcgc ctttgccttc ggggtgccgg
     2401 atcatgaagg gcacgtctgt gatctccggc cagaggacgt aggagggctt tcctgtgtag
     2461 ccgtgctcgc cgaaggccac cccgtggtcg gcgatgacca ggagcagggt gttctcgaag
     2521 aggtcgagct cgtccatcct gtccaggaaa cggccgaacc agcggtctac catagtgacc
     2581 tcggcggcgt agcgggcgtg catcctctgc agctccctct ccgtgagata gtcgctcgag
     2641 ccgtagatca cggagtaggg ctccctgcca tcgtaggggt cgtcgtagag gttgacgtac
     2701 tcgtcggggg tgtcccaggg ctcgtgcggg tcgtagctgt cggccacgag gaagaacggc
     2761 tggtcgcctc ctttggccgc atccagcaac tccatcgctc ccgaaaagac ccgcggggag
     2821 aaccagtcct cttcgctctc ccgttcagcc acaagacgcc cctccacgtt ggcccagtac
     2881 tgctgcatct gggcccgcat cgcggagacg ttgcccttga gcagggcgtt ctgcactctg
     2941 tcgtgaggga aggtccagtt cggcatgtag ttgtcggtgg tctggccccg gatgaagtcg
     3001 aagacgtcga agccctgctg gaagttgtag gaggccttgt actggtgcat gttgtcggtc
     3061 tgtaacaggg tcgcgtagcc gttctgcttc attatctcgg ccaggtgcgt ctgtccctcg
     3121 gggatcggct gccagccctg caggattatg tcctccccct tgggcggctt ccagtccctg
     3181 aaaggccagg tcctgaggcc tgtgtggatg gccctacggg cgcagatggt cggggccgac
     3241 tcggggtagg ctctggtgaa gcgcaggctc tcctttgcca aagcgtcgag gtttggggtc
     3301 tggatccagc cgttgccgta ggcgccgatg tggtccttcc tcaggctgtc cgtgatcacc
     3361 agcaccacgt tcgagccttc gcccgagggc ctgcccggca ggttcgggag gcgatcggtg
     3421 atg
//
LOCUS       DASTUV010000094         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_97293, whole genome shotgun sequence.
ACCESSION   DASTUV010000094 DASTUV010000000
VERSION     DASTUV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97293"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFT03_01985"
     CDS             complement(<1..474)
                     /locus_tag="VFT03_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4846941.1"
                     /translation="MEGLQTVGSGLLTLGLVAVAVALSLYQRLDLEKDIGVAVVRSFV
                     QLAAIGYVIDYVFGLESLGAVALLLAGMVLFAGWTSARRARGVPRSFVVAAGAIGVAA
                     VATLGALLLLQIVPPTARYLIPLGGMVIGNSMNTASLTLTRIRDDVAEQRLKVEAA"
     gene            complement(477..1187)
                     /locus_tag="VFT03_01990"
     CDS             complement(477..1187)
                     /locus_tag="VFT03_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU4846942.1"
                     /translation="MGSLGIRTSGLRYSTGGTEILKGVDAGMPGGSITAVVGPSGAGK
                     STLLRAVNRLIEPTAGEVYLDGEPTSGMDPLALRRRVGMVFQLPALFGATVEEATLYG
                     ARLAGKDADAGRLLEMAGLDASFKDKAPQALSGGEQQRVSIARALALEPEALLMDEPT
                     SALDEAARHRIEDLIRELNASLGLTLVVVSHDLDQVERVADRVVVLVRGRSEGEWGRD
                     EFYSGPGERARRYISGRS"
     gene            complement(1189..1605)
                     /locus_tag="VFT03_01995"
     CDS             complement(1189..1605)
                     /locus_tag="VFT03_01995"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEU4846943.1"
                     /translation="MLWATRRGCHVDRTACVWLIRRFVDAEATFSFFEDPVEAPEGAE
                     LFDVAGARFSHRGDDCSFETFIKEYHLEDAALAEISEIVHDADLMDEKYGRPESDGLD
                     AIIRGMQLVLPDDHTLTGYTDAVFDGLYAYLSREVL"
     gene            complement(1605..2105)
                     /locus_tag="VFT03_02000"
     CDS             complement(1605..2105)
                     /locus_tag="VFT03_02000"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEU4846944.1"
                     /translation="MILIYHLPREPSRHRVAVWRRLKTLGALYLQDGVAALPEDAVTR
                     EQLEWLQLRIREAGGKATLWEALPNTVAENRDLVQAFRAAREESYGKIIEAAHRIRRK
                     AEMGGEGASLLEELARTEREFRAERRRDYFRSPLRTEAAEALRAARRTLVEGAEVRAR
                     EGTEGS"
     gene            complement(2141..>2983)
                     /locus_tag="VFT03_02005"
     CDS             complement(2141..>2983)
                     /locus_tag="VFT03_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU4846945.1"
                     /translation="LYFFSVPFPLVILAAAIGGLLLSRPVPDAFRGGGGHGSSEEEES
                     AVDRAGSNGRPSTLRNLRLLATFLVLWAVPVGAIWIWRGGEDVLVREALFFTGAAFLT
                     FGGAYSVLSYVADVAVNGYGWLTADQMVQGLGLAESTPGPLIMVTQYVGFIGAWNDPG
                     PFSPLLYGTLGALITTYVTFLPCFLFIFLLAPYIELLANDRRLRAILIGVTAAVVGVI
                     ANLAVYFATQVLFPDGYSVGGLDVFALVVAVVSFVVLQRFKVPIYLLVPVGALVGMAW
                     TLLL"
BASE COUNT          535 a         1066 c          924 g          458 t
ORIGIN      
        1 ggccgcctcg accttcagcc gctgctcggc cacgtcgtct ctgatcctgg tgagggtaag
       61 gctcgccgtg ttcatcgagt tgccgatcac catgcctccg agtgggatga ggtagcgggc
      121 cgtcgggggc acgatctgca ggaggagcag cgccccaaga gtcgctacgg ccgccactcc
      181 tatggcccct gccgccacta cgaaggaccg gggcacgccc cgcgcacgac gggcggacgt
      241 ccagccggcg aagaggacca ttcccgccag gagcaacgcg accgcgccca ggctctccag
      301 cccgaagacg tagtcgatga cgtagccgat ggcggcgagc tggacgaagg agcgaaccac
      361 ggcgaccccg atgtccttct ccaggtcaag ccgctggtag aggctcaagg ccaccgccac
      421 ggctacgagc ccgagcgtga gcaggccgga tcccaccgtc tgcagacctt ccattctcac
      481 gacctccccg agatgtaccg cctcgcccgt tcgcctggcc cggagtagaa ctcatccctg
      541 ccccactcac cctcgctccg acctctcaca agtaccacga cgcggtcggc gactcgctcc
      601 acctgatcca ggtcgtggct caccaccacg agcgtgagcc ccagagaagc gttcagctcc
      661 ctgatgaggt cctcgatcct gtgccgggca gcctcatcca gcgcgctcgt cggctcgtcc
      721 atgaggagcg cctcgggttc cagggcaagc gccctggcta tggagacccg ttgctgctcc
      781 cccccggaga gtgcctgcgg cgctttgtct ttgaaagagg cgtcgagtcc cgccatctcc
      841 agcaaccgcc ccgcgtcggc atcctttccg gccagccgcg cgccgtagag agtagcctct
      901 tcgaccgtcg ctccgaagag cgcggggagc tggaagacca tccccacccg ccgcctgagg
      961 gcgagggggt ccatcccgct cgtcggctcc ccgtccaggt agacttcccc tgcggtcggc
     1021 tcgatgaggc ggttgacggc gcgcagcagg gtgctcttgc ccgcccccga tggccccacg
     1081 accgcggtaa tgctgccgcc cggcatcccg gcatctactc ccttcaggat ctccgttccg
     1141 ccggtcgagt accggaggcc cgaagtcctt ataccgagcg acccgatgct agagcacctc
     1201 ccggctcagg taggcgtaga gcccgtcgaa gacggcgtcg gtgtacccgg tgagcgtatg
     1261 gtcgtcgggg aggacgagtt gcatgccccg gatgatagcg tccagcccat cggattcggg
     1321 acgtccatac ttttcgtcca tgaggtcagc gtcgtgcacg atctccgaga tctcggccag
     1381 cgccgcatct tcgagatgat actccttgat gaaggtctcg aacgagcaat catcgccacg
     1441 gtgggagaat ctagctccgg cgacgtcgaa cagctccgct ccctccggcg cctccacggg
     1501 gtcctcgaag aacgagaagg ttgcttccgc gtctacgaaa cgcctgatca gccacacgca
     1561 cgccgtgcgg tccacgtggc accctcggcg ggtggcccag agcatcagct gccctccgtc
     1621 ccctcgcgcg cccgcacttc agcaccctcc acgagggtcc gacgcgccgc gcggagggct
     1681 tcggcggcct cggttcgcaa aggagagcgg aagtagtccc gccggcgctc ggcccggaac
     1741 tcgcgctccg tcctggcgag ctcctctagt agcgaagcgc cttcgccgcc catctccgcc
     1801 ttgcgccgga tgcggtgggc agcctcgatt atcttcccgt aactctcttc ccgggctgcc
     1861 cggaacgcct ggacgaggtc tctgttctcg gcgactgtgt tcggcagtgc ctcccagagc
     1921 gtggccttcc ccccagcctc gcggatgcgg agctggagcc actccagctg ctcgcgcgtg
     1981 acggcgtcct ccggcagggc ggcgaccccg tcttgcaggt agagggcgcc cagagtcttg
     2041 agcctgcgcc atacggccac cctgtggcgc gaaggctccc tggggaggtg gtagatcagg
     2101 atcacccacc gcgtcttctc ccggcctgac ggtccgttgc ctacaggagg agcgtccacg
     2161 ccatcccgac cagagcaccc accggcacaa gcaggtagat agggaccttg aacctctgca
     2221 acaccacgaa cgagacgaca gcgaccacaa gggcgaagac gtcgagaccg cccacagagt
     2281 agccgtcggg gaacaggacc tgcgtggcga agtagaccgc aaggttggcg atcaccccca
     2341 ccaccgcagc cgtaaccccg atgaggatgg ccctcaggcg acggtcgttt gccagcaact
     2401 ctatgtaggg cgccaggagg aagatgaaca ggaagcacgg caggaacgtg acgtacgtcg
     2461 tgatcaaagc gcccagcgtc ccgtagagga gcggagagaa gggtccgggg tcgttccagg
     2521 cgcctatgaa gccaacgtac tgggtgacca tgatgagagg ccccggcgtg gactccgcaa
     2581 gccccagtcc ctgcaccatc tggtccgcgg tgagccaccc gtagccgttg accgccacgt
     2641 cggcgacgta gctcaacacc gagtatgccc cgccgaaggt caggaacgcc gctcccgtga
     2701 agaagagcgc ctcacgaacc agcacgtctt ccccgccccg ccagatccag atagccccca
     2761 ccggcacagc ccacagtacc aggaaggtag ccaacagcct caggttgcgc agcgtggagg
     2821 gccgtccgtt cgagccggcc cgatccacgg ctgactcttc ctcctccgag gagccgtgcc
     2881 cgccgcctcc ccgaaaagcg tctggtaccg ggcggctcag cagcaacccg cctatggcgg
     2941 cggccaagat gaccagtggg aaggggacgg agaagaaata cag
//
LOCUS       DASTUV010000095         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1205416, whole genome shotgun sequence.
ACCESSION   DASTUV010000095 DASTUV010000000
VERSION     DASTUV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205416"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFT03_02010"
     CDS             complement(<1..448)
                     /locus_tag="VFT03_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846946.1"
                     /translation="MEARADQKGLKGGALNTVEAMIVSMGLMAPSVAMFFNTPFIAQF
                     AGAAMPLAMLFSFAAVFLVALGFSQLAQRTASAGNVYAFVSNAINPKTGFMGMWLFII
                     GYGIFEAATYSIFAAFSSEELNRIFGLSVPWFIPFFVIAGLVYTLSV"
     gene            complement(720..1361)
                     /gene="nth"
                     /locus_tag="VFT03_02015"
     CDS             complement(720..1361)
                     /gene="nth"
                     /locus_tag="VFT03_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4846947.1"
                     /translation="MQSTPYSAPISEVISRLKDEYPDARTELTWENPLELLVATMLSA
                     QTTDVRVNMVTDKLFEKYRTAADYAGADPAELEADIRPTGFYRNKARSLQGMARALLD
                     EHGGEVPRTMAGLVSLPGVGRKTANVVLGNAFGVDEGVVVDTHVRRVSRRLGLTAEKD
                     PEKIEQDLLGVVPEGERTLFSHLLIFHGRRVCKARKPDCPGCVLSDICPSSTV"
     gene            1435..>2598
                     /locus_tag="VFT03_02020"
     CDS             1435..>2598
                     /locus_tag="VFT03_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU4846948.1"
                     /translation="MAEVTFDYRNLMEVEGGLRSDELDEVRPRLQQAVDELLGSPPGF
                     MRLPKTSEYAEASVGVAEEILSSGATDFIHVGIGGSALGPMAIQKALNDPYYNALPTE
                     DRRGPRLHFAENTDPNTLAAILDFADATGTWVNVVTKSGSTAETMANFLVIRGWLVEA
                     LGDFGYQERTIATTDPEEGYLKEIADREDLKLLPIPKDVGGRFSVLTPVGLLPASVTG
                     LDVRALLAGAAQCVDEVNEQGAEHPAVVGAAMHYLMDTTRGRNVRVMMPYADALERLA
                     AWFVQLWAESLGKDGKGSTPHGAVGTTDQHSQIQLYMQGPQDKIVEIVEVENHPRDLE
                     IPRAYEDLDGVAYLGGHTLAELLNVECDATRKALTEAGRPNSTIRLGTVSEENL"
BASE COUNT          501 a          835 c          848 g          414 t
ORIGIN      
        1 gaaccgacag cgtatagacc agtcccgcga tgacgaagaa aggaatgaac cacggcacgc
       61 tcagaccgaa gatgcggttc agctcctcgc tcgagaacgc ggcgaagatc gagtaggtgg
      121 cggcctcgaa gatgccatac ccgatgatga agagccacat ccccatgaag cccgtcttgg
      181 ggttgatggc gttggacacg aacgcgtaga cgttccccgc agacgcggtt ctctgggcta
      241 gctgcgagaa gcccagagct accaggaaca ccgccgcaaa cgagaacaac atcgcaagcg
      301 gcatcgccgc gccggcgaac tgggctatga agggggtgtt gaagaacata gccaccgaag
      361 gggccatgag ccccatcgaa acgatcatcg cttccaccgt gttgagcgcg ccgcccttca
      421 gacccttctg atccgccctc gcctccatct ctcgcctcct ctcgggtgtc tgaacacccc
      481 agacgcatgt tatgtgccgt acataatagc tatccggcaa taccgttgca cgaacacacc
      541 cggcccgctc gagtggcggt ttcggtcctg ttagagtcgc gccagggaag ggccttcgag
      601 gagtagcgga tgcttcgagc cctaccagct tgtcgcgcac gcgaagcggc ctcacggctg
      661 ctgcccacac accgataaat cggcgatgaa tcgaggatga acccgcgtga agaagtgctc
      721 taaacggtgc tcgaaggaca gatgtcgctc aggacgcagc cagggcagtc gggcttgcgg
      781 gccttacaga cgcggcggcc gtggaagatg aggaggtgcg agaagagggt acgctctcct
      841 tctggcacca ccccgaggag gtcctgctct atcttctccg ggtccttctc tgcggtgagg
      901 ccgagcctgc gcgagacgcg cctgacgtgg gtgtcgacga cgacgccctc gtccacgccg
      961 aaggcgttgc cgaggaccac gttggcggtc ttgcgcccga cgccggggag ggagacgagc
     1021 ccggccatgg tgcgggggac ctctccgccg tgctcgtcga gcagggcgcg cgccatcccc
     1081 tgcagggaac gcgccttgtt gcggtagaag ccggtcggac ggatgtccgc ttcgagctcc
     1141 gcagggtccg ctcctgcgta gtcggcggcg gtgcggtact tctcgaacag cttgtcggtg
     1201 accatgttta cgcgcacgtc ggtcgtctgg gcggagagca tggtcgctac cagcagctcc
     1261 agggggttct cccaggtcag ctcggtcctg gcgtcagggt attcgtcctt caggcggctt
     1321 atgacctcgg agatcggggc ggagtatgga gtactttgca cgatggccgg gcattatacc
     1381 gttgaataaa cttaccggtt cagacgggag accgcgacag aggggagccc agagatggcc
     1441 gaagtcacct tcgattaccg aaacctcatg gaggtggagg gcggcctgag gagcgacgag
     1501 ctggacgagg tgcggcctcg cctgcagcag gccgtggacg agttgctcgg gagccctccc
     1561 ggcttcatgc ggctgccgaa gaccagcgag tacgccgagg catcggtggg agtggcggag
     1621 gagatcctga gctccggggc cacggacttt atacacgtcg ggatcggggg ctcggctcta
     1681 ggcccgatgg cgatccagaa ggcgctcaac gacccttact acaacgcgct tcccaccgaa
     1741 gaccgccgcg ggccccggct gcacttcgcg gagaacaccg accccaacac tctggccgcc
     1801 atactcgact tcgcagacgc cacgggcacc tgggtcaacg tcgtcaccaa gtcgggctcg
     1861 accgcggaga cgatggccaa cttcctcgtc atccgcggct ggctcgtgga ggctctgggc
     1921 gatttcggct accaggagcg gaccatcgca accaccgacc cggaggaagg ctacctcaaa
     1981 gagatcgccg accgcgagga ccttaaatta ctgcctatcc ccaaggacgt cggcggcagg
     2041 ttctccgtcc tcacacccgt cgggctgctg ccggcttccg tcacggggct ggacgtccgt
     2101 gcgctgctcg cgggcgccgc ccagtgcgtg gacgaggtga acgagcaggg cgccgagcac
     2161 cccgcggtcg tcggcgcggc gatgcactac ctcatggaca cgacgagggg ccgcaacgtc
     2221 agggtcatga tgccctacgc cgacgccctc gagaggctcg ccgcctggtt cgtccagctc
     2281 tgggcggagt ctctgggcaa agacggcaag ggctcgacgc cccacggcgc tgttggcacc
     2341 accgaccagc actcccagat ccagctctac atgcagggcc cgcaggacaa gatcgtcgag
     2401 atcgtcgagg tcgagaacca cccgcgcgac ctggagatac ccagggctta cgaggacctc
     2461 gatggcgtcg cctacctcgg cgggcacact ctggccgagc tcctcaacgt cgagtgcgac
     2521 gcgacgcgaa aggccctcac agaggccggc aggcccaact cgaccataag gctcggcacg
     2581 gtgagcgagg agaacctc
//
LOCUS       DASTUV010000096         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_285736, whole genome shotgun sequence.
ACCESSION   DASTUV010000096 DASTUV010000000
VERSION     DASTUV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285736"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFT03_02025"
     CDS             complement(<1..229)
                     /locus_tag="VFT03_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HEU4846949.1"
                     /translation="MRVREFAKTVDHTLLKPDTREQDIVRLCEEAARYHFAAVCVPPC
                     YVRLAAQELRGADVKVATVVSFPFGYDATPVK"
     gene            complement(273..1193)
                     /gene="era"
                     /locus_tag="VFT03_02030"
     CDS             complement(273..1193)
                     /gene="era"
                     /locus_tag="VFT03_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU4846950.1"
                     /translation="MSDSIAEGIENGFRSGFVAVVGRPNVGKSTLVNRLVGQKVSITS
                     PRPQTTRGPIRGVRNGPDYQAVFVDTPGSQKPRDTLRARMQQQVLDSLSESDAIFFVL
                     DATQATDGIGPGDRYVARLVAESGTPAIAVINKVDLLRNREGVLPIVEEVSELGDWRE
                     IFPVSAAEGLNIEPLMEAVIELLLPGPRYFPEGKITDYPESLILAEYVREKALNVLRE
                     EIPHAVAVEIEEVERKESVTVVYATIHVERPTQRMIVLGKGGKTIKRIGVEARRDVER
                     LLGNRIYLDLKVKVSPGWRSDKKFLERLGL"
     gene            complement(1190..1579)
                     /locus_tag="VFT03_02035"
     CDS             complement(1190..1579)
                     /locus_tag="VFT03_02035"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU4846951.1"
                     /translation="MDLDAVMDAARDASRNAYAPYSNFRVGAAILTEGGQIHAGCNVE
                     NASYGLAICAERNAAFAMAFANPEDREIRLVAVFSPDAAPCFPCGACRQVLREFGCKE
                     VVVEEAAGLRSYPFDEILPYSFGPEDL"
     gene            complement(1590..2324)
                     /locus_tag="VFT03_02040"
     CDS             complement(1590..2324)
                     /locus_tag="VFT03_02040"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU4846952.1"
                     /translation="MANKSKHPLKGQQGLARSFDHAYRGLIYAVRTQRNMRFHDVVAT
                     LGLVGSLFLGVSKLELAALVLVIMFVFVTEMFNTALEFAVDLAIKEYHPLAKLAKDVS
                     AGAVLVSSVGAVLVGYLILADDLGPLSLETIDTIRHRPAHLTLVVLGVVVLLVLFGKA
                     VTQSPSSFAGGMPSGHAAVAFAGWVAASFVAADGRYGGLVSAITLLMALLVCQSRVES
                     GTHTLYQVATGAVIGILVTVAAFQLL"
     gene            complement(2308..>2676)
                     /gene="ybeY"
                     /locus_tag="VFT03_02045"
     CDS             complement(2308..>2676)
                     /gene="ybeY"
                     /locus_tag="VFT03_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4846953.1"
                     /translation="ALDPELATRIAATAFYCRGFDVERLGEVSVALVDPGVIRDLNSR
                     FRGKDEPTDVLSFEVDGPYGEMVGEVVICPACAEMGVEELIVHGALHLSGMDHGEDFE
                     GSEMARAQEVVIRVARGEQE"
BASE COUNT          472 a          878 c          840 g          486 t
ORIGIN      
        1 cctttaccgg cgtagcgtcg tagccgaacg gaaacgaaac caccgtcgca accttgacat
       61 ccgccccgcg tagctcctgg gcggcgagcc ggacgtagca cggcggcacg cacaccgcgg
      121 cgaagtggta gcgggccgcc tcctcgcaca ggcggacgat gtcctgctcc ctcgtatcag
      181 gcttcaggag ggtgtgatcc accgtcttcg cgaactctcg caccctcatc gccacgaccc
      241 aggctcctca ctttactgca cgacattagt aattataacc ccagccgctc caggaacttc
      301 ttgtcgctcc tccatcccgg gctcaccttt accttgaggt cgaggtaaat gcgattaccg
      361 aggaggcgtt cgacgtcgcg gcgagcttcg acgccgatac gcttgatggt cttgccgcct
      421 ttgccgagca ctatcatgcg ctgtgtggga cgttcgacgt ggatcgtggc gtagacgact
      481 gtaacgctct ccttacgctc gacctcttcg atctcgacgg ccacggcgtg cgggatctcc
      541 tccctgagga cgtttagcgc cttctccctg acatattcgg cgaggataag cgactccggg
      601 tagtcagtga tcttgccctc agggaagtag cgtggtcctg gtagtagaag ctcgatcaca
      661 gcctccatca acggctcgat gttcagccct tcggcggcgc ttaccgggaa gatctcgcgc
      721 caatccccga gctccgatac ctcctccacg atgggaagta ccccctcgcg gttgcggagt
      781 agatctacct tgttgatcac ggcgatggcc ggggttcctg actcggcgac gaggcgagcc
      841 acgtagcggt cgccggggcc tatcccgtcg gtcgcctgcg tggcgtcgag gacgaaaaag
      901 atcgcgtctg actccgagag gctgtcgagg acctgctgtt gcatcctggc gcgcagagtg
      961 tcgcggggct tctgggagcc tggcgtgtcc acgaagaccg cctggtagtc ggggccgttg
     1021 cggacgcccc ttatcgggcc gcgggtggtc tggggtctgg gcgaggtgat agagaccttc
     1081 tgcccgacca gcctgttgac gagcgtggat ttccccacgt tcggccgccc gacgaccgct
     1141 acgaagccgc ttcgaaagcc gttctctatc ccttcagcta tgctgtcgct cacaggtcct
     1201 ccgggccgaa agagtagggt agtatctcat cgaacgggta gctgcggaga ccggccgctt
     1261 cctcgacgac gacctccttg cagccgaact cgcgcaggac ctgccggcag gctccgcagg
     1321 ggaagcaggg tgcggcgtcg gggctgaaga ccgcgacgag ccggatctca cggtcctccg
     1381 ggttcgcgaa ggccatggcg aaggcggcgt tacgctcggc gcatatggcg aggccgtaag
     1441 aggcgttctc gacgttgcag cccgcgtgga tttggccgcc ctcggtgagg atcgccgcac
     1501 ctacccggaa gttgctgtac ggcgcgtagg cgttcctgga ggcgtcgcgg gccgcgtcca
     1561 tcacggcatc gaggtccata cccctccttt caaagcagtt gaaaggcagc gaccgtgacg
     1621 agtatcccta tcacggctcc tgtagccacc tggtagaggg tgtgggtgcc gctttcgacc
     1681 cggctctggc agacgagcag ggccatgagc agcgtgatgg cggataccag cccaccgtat
     1741 cgaccgtcag cggcgacgaa gctcgcggcg acccacccgg cgaaggcgac cgcagcgtgc
     1801 cccgagggca tccctccggc gaaggagctg ggagactgcg tgacggcctt cccgaatagc
     1861 acgagcagga ccactacccc gagcacgacg agcgtgaggt gcgcgggccg gtggcgtatg
     1921 gtgtcgatag tctcgagcga gagcggaccg aggtcgtccg ccaggatcaa atatcctacg
     1981 agcacagccc cgacgctcga taccagcacc gcccccgccg aaacgtcctt cgcgagcttc
     2041 gccagcggat ggtactcctt gatggcgaga tccacggcga actcgagggc cgtgttgaac
     2101 atctccgtga cgaagacgaa catgatcacg aggaccaggg ccgccagctc gagcttgctc
     2161 acccccaaga acaggctacc gacgagtccc agcgtcgcca cgacgtcgtg gaagcgcatg
     2221 ttccgctggg tgcggaccgc gtagattagc ccgcggtagg cgtggtcgaa gctgcgagct
     2281 aagccctgct ggcctttgag ggggtgctta ctcttgttcg ccacgcgcca cccttatcac
     2341 gacctcctgc gcgcgggcca tctcgctccc ctcgaagtcc tccccgtgat ccatccccga
     2401 gaggtgcaat gcgccgtgaa ctatcagctc ctcgaccccc atctcagcgc aggcggggca
     2461 tatcactacc tctccaacca tctccccgta cggcccgtca acctcgaagc tgagcacgtc
     2521 ggtcggctcg tccttacccc tgaatcttga gttgaggtcg cggatgacgc cagggtccac
     2581 gagggctacc gagacttcgc caaggcgttc aacgtcgaag ccgcggcagt agaaggccgt
     2641 ggcggcgatc cgggtggcca gctcggggtc gagtgc
//
LOCUS       DASTUV010000097         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_740237, whole genome shotgun sequence.
ACCESSION   DASTUV010000097 DASTUV010000000
VERSION     DASTUV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740237"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1027)
                     /locus_tag="VFT03_02050"
     CDS             complement(218..1027)
                     /locus_tag="VFT03_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4846954.1"
                     /translation="MAPTGMYIEANGLEVYYEHSGEGEPLLLVHGGTATSQSWASHLP
                     AFTEHFEVFTPDSRGHGRTDNPTGELAYHVMADDLASLIDALGLQRPLVLGYSDGGQI
                     ALELGLRYPGLARALVLGGTQFRFSEAYLEAVRSLLGITEDEELDPEKLEREQPDWVA
                     YLREAHGHVYGPEYWKAYVKQIAALWLKPVRYTPEDIGAVADPVLLLAGDRDGVCTEE
                     SIELFGVLPNAELAVAPASDHGFIEAKAGLFDALALDFLMRHRNDSARNSK"
     gene            1287..1793
                     /locus_tag="VFT03_02055"
     CDS             1287..1793
                     /locus_tag="VFT03_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU4846955.1"
                     /translation="MTAKEGWRGRFFEDFEVGDVYEHPSGRTVITTDNMWFTLLTQNT
                     APIHVDHAYAAQTEFGKPLVDSTFTLALVTGQSVTDVSQNVFANLGWDEVRLPAPVFE
                     GDTIYSRSEVLEKRESRSRPNVGIVVVKTTGYNQNGETVITFNRTIMVYKTEHAPKVD
                     RTFPEERA"
     gene            1876..2361
                     /locus_tag="VFT03_02060"
     CDS             1876..2361
                     /locus_tag="VFT03_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4846956.1"
                     /translation="MELHVLRTSMSLPLSREQVFAFFADAANLELITPPELRFRVLTR
                     QPILMQEGTLIDYRLRLFRVPLRWRARITRWQPPFGFVDEQVRGPYRVWEHTHLFREE
                     GGATTIEDVVRYELPVAPFGEIFHPLVRLQLERIFRFRRSAVRNCLLGEPDARLQTLT
                     S"
     gene            complement(2442..>3052)
                     /locus_tag="VFT03_02065"
     CDS             complement(2442..>3052)
                     /locus_tag="VFT03_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU4846957.1"
                     /translation="EEARKVEIVRSPNIVPPPEGAPLPEVLEGRVVIVVGDDVSTGDM
                     APDGTLGMSLWSNIPECAKYMFRRQDPEFHDRALEWGGGFIVGGHNYGQGSSREQAAL
                     APLHLGISAVIAKNFARIHRRNLISQSILPLLFREEDDYKRINQGDRWKIEGVRETIT
                     AGGQTLVVNTDDEREISLEIRLSPREREVLLAGGTLNFLRSA"
BASE COUNT          561 a          953 c          945 g          593 t
ORIGIN      
        1 cacaactccc cccgggcgga gggaccaggt accttgccgc ccgcgatcgc gccgcccggc
       61 tccggatgcc cctcggggcc ggagtccgtc tgcacgtctc tgagcactac cggagcagca
      121 cgacctcgct cgaggtgcgt ccgagctccg gtaactcgag caatatttcg actgctggat
      181 ggtgaggttt cgggcttgca cggcgactcc gcaagcgcta tttggaatta cgagctgagt
      241 cattccgatg ccgcatgagg aaatccaggg cgagcgcgtc gaatagtccc gctttcgctt
      301 cgatgaagcc gtgatccgag gccggagcga cggcgagttc tgcgttgggg agtacgccga
      361 acaactcgat ggactcctcg gtacataccc cgtcacggtc accggcgagg agaagcaccg
      421 ggtcggcaac cgcaccaatg tcctcgggcg tgtaacgcac gggcttcagc cacagcgctg
      481 cgatctgctt tacgtacgcc ttccagtact ccgggccgta gacgtgcccg tgcgcctcgc
      541 gcaggtaggc aacccagtcg ggttgctccc gctccagctt ctcgggatcc aactcctcgt
      601 cctcggtgat ccccaggagt gaacggacgg cctcgaggta cgcttctgaa aacctgaact
      661 gtgtgccgcc caacaccagg gctctggcca ggccggggta gcgcaagccg agctcgaggg
      721 cgatctgccc tccgtcgctg tagcccagaa cgagcggcct ctgcagaccc agcgcatcga
      781 tcagggaggc caagtcgtcc gccatcacgt ggtaggcgag ctcgcccgtc gggttgtcgg
      841 tcctgccatg accgcggcta tctggggtga aaacctcgaa gtgctcggtg aaggccggaa
      901 ggtgcgatgc ccaggactgc gaggtcgctg tgccgccgtg aacgagcaat agtggctcgc
      961 cctcgccgga gtgttcgtaa tagacctcga gcccgttggc ctcgatgtac attcctgtgg
     1021 gtgccatcag cgttctgctc cgcacgtggt tgcgttcgtc tagcttccta cagatccgtc
     1081 tgaatggaaa gcgtagaggc cgtactgccg aaacggaatc gattcgaatt ttggcgaatg
     1141 gtgttgagac gctgaccaac cgcaaatcat gcacgccttg atcgtgcagc gtcgccaggg
     1201 ttgcctgtag tattgacgat tgcagcgccg aaccgctcct tcgatcgggg acggcggagt
     1261 gactcttgca aatgagagga gagcgtatga cggcgaagga gggctggcga ggccgcttct
     1321 tcgaggattt cgaggttggc gacgtctacg agcatccgtc agggcgtacg gtcataacca
     1381 cggacaacat gtggttcacg ctcctcaccc agaacacggc tccaatccac gtggatcacg
     1441 cctacgcggc ccagacggag ttcggcaagc cgctcgttga ctcgaccttc acgctggcgc
     1501 tggtaacggg ccaaagcgtc accgatgtct cccagaacgt ctttgccaac ctgggatggg
     1561 acgaggtcag gctgccggca ccggtcttcg agggtgacac gatctactcc aggagcgagg
     1621 tgctcgagaa gcgcgagtcg cgctcgcgac cgaacgtggg gatagtggtg gtgaagacga
     1681 ccggctacaa ccagaacggg gagacggtca tcacgttcaa ccgcaccatc atggtctaca
     1741 agacggaaca cgctccgaag gtcgatcgca ccttccctga agagcgcgca taaggatgcc
     1801 gcgaatgcga gcgcctggcg caggaccgga ggtgggtacc gtgccggggc aactcgtctg
     1861 gctacaatga ggagcgtgga gctccacgtt ctcaggacat ccatgagctt gccgctgtcg
     1921 cgcgagcaag tgttcgcctt cttcgccgac gccgccaacc tggagctcat caccccgccc
     1981 gaactccgat ttcgagtact cacgcggcag ccgatcctga tgcaagaagg aaccctgatc
     2041 gactacaggc tccgcttgtt cagagttccg ttgcgctggc gggcgcgcat cacgcgctgg
     2101 cagccgcctt tcgggttcgt ggacgagcaa gtgcgcggtc cataccgtgt ctgggagcat
     2161 acccatctgt tccgcgagga aggcggagcg acgaccatcg aagacgttgt gcgctacgaa
     2221 ctgccggtgg cgccgttcgg cgagatcttc catcctctgg taaggctaca actcgagcgg
     2281 atcttccggt ttcgaagatc cgcagtgcga aactgcctcc taggagaacc tgacgcacgt
     2341 ttgcaaaccc taacgtcata atggctgata tcgagcctgg tgttctcctc tcgaagaacc
     2401 gtcccctccg gccctatttc accgccacgc cgatgccttt cctatgcgct gcgcaagaaa
     2461 ttgagcgtcc cgccggccag gaggacctcg cgctctcggg gtgagagtct gatctccagg
     2521 cttatctctc tctcgtcgtc ggtgtttacc acgagtgttt gccctcccgc ggtgatcgtt
     2581 tcacgcacgc cctcgatctt ccacctatcg ccctggttga tccgcttgta gtcgtcctct
     2641 tcgcggaaaa gcagcggaag gatgctctgg gagatcaggt tccggcggtg gatgcgggcg
     2701 aagtttttgg ctattaccgc gcttatgccg aggtgcagag gcgcgagggc ggcttgctcg
     2761 cggctggagc cctgcccgta gttgtgcccg ccgacgatga aaccaccgcc ccactcgagc
     2821 gcccggtcgt ggaactccgg gtcctgccgg cgaaacatgt acttcgcgca ctctggtatg
     2881 ttcgaccaga ggctcatacc tagagtcccg tcgggagcca tatccccggt cgagacgtcg
     2941 tctccgacga ctatgacgac acgtccctca agtacttcgg ggagcggtgc gccctcgggt
     3001 gggggcacga tgttggggct gcggacgatc tcgacttttc gcgcctcctc cg
//
LOCUS       DASTUV010000098         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1776943, whole genome shotgun sequence.
ACCESSION   DASTUV010000098 DASTUV010000000
VERSION     DASTUV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776943"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..493
                     /locus_tag="VFT03_02070"
     CDS             314..493
                     /locus_tag="VFT03_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846958.1"
                     /translation="MKDALYTIALKNADRYEALARRAAMSSDEELAEFFRRMRDESRE
                     NAERAKRLLSQRVAE"
     gene            520..1401
                     /locus_tag="VFT03_02075"
     CDS             520..1401
                     /locus_tag="VFT03_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4846959.1"
                     /translation="MITDNTSIDYDVHGEGPPLLLIGGLGFGRWAWFRQVPAFSRHFK
                     TIVFDVRGERDLRGGVADLVSDVVTLLEHLRVKKAHVLGTSLGGFVAQELALERPDLV
                     DRLVLVCTSYGRGGPETMSPWALADMIGLPSLNSEKAVRQGLEAATSDAYRAERSEEF
                     EQIVRWRLADSPSLSTYYEQVRAGARFDVSRDVGRITSPTLVIHGSEDQYVPAANAVA
                     LAEAIPGSRLRVLEDAGHLVFIERYADVNREVVTFLKPRRSRRRGRGHDAPAKGIGEP
                     LRRAVRRLVEGARAKGS"
     gene            1489..>2033
                     /locus_tag="VFT03_02080"
     CDS             1489..>2033
                     /locus_tag="VFT03_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846960.1"
                     /translation="MSERGVGNDGERGEAAAPVAPMAPFDVWTQWLKSNMGEVMAPPG
                     VSVPWLVPAGINTGEEAEPLPEGALRNDPMLQVVEQLWNANPLQNVLPINWVEITRAL
                     QTLWAREMSDPAAAAQRAAEYNRRFFETTMEVWSDATARFWGLPQQEKEEENRQDKRF
                     AGPEWESNPFYQTLKESYLLAS"
BASE COUNT          399 a          587 c          715 g          332 t
ORIGIN      
        1 ttattcacac tacgtatata agttcttctt tattatatgt gcacagcgca gataatcttg
       61 ctgtagcctt gaaggctcac ctgttatcga gaaggaggtt ggtgaccgat gaagggcatc
      121 ttcaagcgca gatcggtggg agccaccggc aaggccggcg acaatgtacg cgcgcggcgc
      181 aagcccccgg tacgcgagag gcacctggtg cgcggttagg ggtgccggcc ggtaaccgga
      241 ctcggagggc cgagacatca gtctcggcct cctgtccgtc aggcgacgtg cgacgatcgg
      301 gaggtcaaca gacgtgaagg atgcgctcta cacgatagcg ttgaagaacg cggacaggta
      361 cgaggcgctt gccaggcgtg ccgcaatgtc cagtgatgaa gagctcgccg agttcttccg
      421 caggatgcgg gacgagagcc gcgagaatgc cgagagggcg aagaggctgc tctcccagag
      481 ggtagcggag tagctccggg aggtagtgac gtgccggtga tgatcaccga taacacgagc
      541 atagactacg acgtccacgg agaaggacct cccctgctct tgatcggggg gctgggcttc
      601 gggcgctggg cgtggttcag gcaggtccct gccttttcgc gccacttcaa gaccatcgtc
      661 ttcgacgtca ggggggagcg ggacttgaga ggtggggtcg ccgacctcgt ctccgacgtg
      721 gtgacgctac tcgagcacct gcgcgtaaag aaggcccacg tcctcggcac ctctctgggc
      781 ggcttcgtgg ctcaggagct ggcgctggag aggcccgacc tcgtagaccg gctggtgctg
      841 gtatgcacga gctacggtcg tgggggtccc gagaccatgt cgccctgggc gctggcagac
      901 atgatcgggc taccctccct caactctgaa aaggcggtca ggcagggtct cgaggcggcc
      961 acgtcggacg cgtatcgcgc cgaacgatcg gaagagttcg agcagatagt gcgctggcgc
     1021 ctggcggact cgccttccct gtctacctac tacgagcagg tgagggctgg tgccagattc
     1081 gacgtctcgc gcgacgtggg gcgcataacc tctccgacgc tcgtgatcca cggctcggag
     1141 gaccagtacg tgccggctgc caacgccgta gcgcttgccg aggcgatacc cggctccagg
     1201 ctgcgcgtcc tcgaagatgc cgggcacctg gtctttatcg agcggtacgc cgacgtcaac
     1261 agggaggtcg tcaccttcct caagccgcgc aggtcacgga ggaggggtag ggggcatgac
     1321 gctcccgcga agggtatcgg ggagccgctc cggagggcgg tgcgccgcct ggtcgaagga
     1381 gcgagggcca aggggtcctg acaaataccc ggctatgcta ggaaacccgg cgaaacgggt
     1441 aaagaagcaa gaagtgaacc gagtatccga gaacattgag gaggacccat gagcgaacgc
     1501 ggagttggca acgacggcga gagaggtgag gcggccgcgc cggtcgcgcc gatggcgcct
     1561 ttcgacgtgt ggacacagtg gctcaagagc aacatgggcg aggtgatggc gccgcccggg
     1621 gtcagcgtcc cctggctcgt gcctgccggc atcaataccg gtgaggaagc tgagcctctg
     1681 ccggagggag ctctaaggaa cgatcccatg ctccaggtcg tggagcagct gtggaacgcc
     1741 aaccccttgc agaacgtgct gccgatcaac tgggtggaga tcacgcgcgc gctgcagacg
     1801 ctgtgggcgc gcgagatgag cgaccctgcg gccgccgcgc agcgggccgc cgagtacaac
     1861 cggcgcttct tcgagaccac gatggaggtg tggagcgacg cgacggcccg cttctggggt
     1921 ctgcctcagc aagagaagga agaggagaac agacaggaca agcgcttcgc cgggcccgag
     1981 tgggagagca atcccttcta ccagacgctc aaggagtcgt acctgctcgc ctc
//
LOCUS       DASTUV010000099         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_238842, whole genome shotgun sequence.
ACCESSION   DASTUV010000099 DASTUV010000000
VERSION     DASTUV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238842"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..>2352
                     /locus_tag="VFT03_02085"
     CDS             184..>2352
                     /locus_tag="VFT03_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU4846961.1"
                     /translation="MGIILLCALVAGVAFAAGGYVGLIRSVDRLGEPRNAETHPTYIY
                     SAPLGGNGGSRRVIGTIFQGQNIKTASKEEMPIYLLNALVAKEDERFMEHGGVDLWGI
                     MRALWVDIRAGETVEGASTITQQYVKNAYLSHDQTWRRKIKEALIAIELERKNEDKDQ
                     ILADYLNTVYFGNNAYGVQAASETYFNKSVEDLTVAESATLIGLLWSPSTLGQDREAA
                     KYQRDLVLNKMAEAGYISRPDQISALEEPMPQRWPMTPMVESGLTGPSQTRDFAEMVQ
                     EELISKYGVNTVLQGGLSVYTTLDLEAQVTAREILYAPDGYLPSPQSPDAALVSIDPE
                     TGHITAMVGNRDPNSQFNLVTQGRRQPGSSFKPYALIAALEQGVDPDTKFVSEKKVYQ
                     VDVGTDEPERWRVKNYDGIERGTISLREALWWSDNTVFTDLVMNADGKGLENGPEAIA
                     DVANRLGISTNFPDHPNPSIVLGAYEVSPLDMATAYATIANGGRRVEPTMISRVVSDE
                     GKEGEKVLYEAPEHPEEEQVIDPEIAHEATEIMIGDVTQGIAEDASLGGRPVAGKTGT
                     SENFFDAWFIGFTPQLLTGIWMGYADGGQTLEYDLEYARELNGLYGGITPAEIWKTYM
                     EQMLEGQPIEQFEGVEMPTQEQTTPANAETPGTEPSGAAGPRAVEPLPAGILGDRPTN
                     VEPSSASPSSAAPSSVPPSSAAPSSAAPSSASPSSANPSRR"
BASE COUNT          504 a          747 c          749 g          352 t
ORIGIN      
        1 gtaactcgcc tcgacctagt gtagtatacc ggctccatgg ctcgcaccta tcagagtgga
       61 gggggacgga gcagcactgg ggcgcggcga cggcccgcct ccaaacggcc agtccggctc
      121 ccggcgtcca agaagaagcg aggcttcccg tacggaaacc tgaaggtcag gatcctgcgc
      181 ggcgtgggca tcatcttgct gtgtgctttg gtagccgggg tcgcctttgc tgctgggggt
      241 tacgtgggtt tgatccggag cgtcgatcgg ttgggcgagc ctcgaaacgc cgagacgcac
      301 ccgacgtaca tatactccgc accgcttggc ggcaacgggg gctcgcggcg tgtgatcggg
      361 acgatcttcc agggtcagaa cataaagacc gccagcaagg aggagatgcc gatctacctg
      421 ctcaacgcgc tcgtcgccaa ggaagacgag cgcttcatgg agcacggcgg agtggacttg
      481 tgggggatca tgcgggcgct atgggtggac atccgggccg gcgagaccgt cgagggggcg
      541 agcacgataa cccagcagta cgtcaagaac gcctacctca gccacgacca gacttggcgg
      601 cgcaagatca aggaagccct catcgccata gagttggagc gcaagaacga ggacaaagac
      661 cagatcctcg ccgactacct gaacaccgtg tacttcggca acaacgccta cggtgtccag
      721 gcggcctcag agacctactt caacaagtcc gttgaggacc tgaccgtcgc cgagtccgcg
      781 accttgatag ggctcctctg gtcgccatcg acccttgggc aggaccggga agcggccaag
      841 taccagcgcg acctcgtgct gaacaagatg gccgaggcag gctacataag cagacctgac
      901 cagataagcg cgctcgagga gccgatgccg caaaggtggc cgatgacgcc gatggtagaa
      961 tcgggtctca cggggccgag ccagacccgc gacttcgcgg agatggtgca ggaggagttg
     1021 atcagcaagt acggggtcaa caccgtcctg cagggcggcc tgagcgtata cacgaccctc
     1081 gacctcgagg cgcaggtgac ggcgcgcgag atcctctacg cgccggacgg gtacctaccc
     1141 agcccccagt ctcccgacgc cgcgctcgtc tcgatagatc cggagaccgg ccacataacg
     1201 gcgatggtcg gcaacaggga cccgaattcc cagttcaacc tggttaccca gggcaggcgc
     1261 cagccaggca gctccttcaa accttacgct ctcatagcag cgctggagca gggcgtcgat
     1321 cccgatacca agttcgtctc ggagaagaag gtctaccagg tcgacgtggg gacagacgag
     1381 cccgagcgct ggcgggtcaa gaactacgac gggatcgagc gcggcaccat cagcctcaga
     1441 gaagccctgt ggtggtcgga caacacagta tttaccgacc tggtgatgaa cgccgatggc
     1501 aaggggctcg agaacggccc ggaggcgatc gcggacgtag ccaaccggct cggcatctcc
     1561 accaatttcc cagaccatcc caatccttcg atcgtcctgg gcgcctatga ggtctcgccg
     1621 ctcgacatgg ctaccgccta cgcgaccata gccaacggcg ggcgccgggt ggagccgacc
     1681 atgatctcca gagtggtaag cgacgagggt aaagagggag agaaggttct ctacgaggcg
     1741 ccagagcatc cagaggaaga gcaggtgata gatcccgaga tagcccacga ggctaccgag
     1801 atcatgattg gcgacgtcac ccagggcata gccgaggacg cctccctcgg cggccggccc
     1861 gtcgccggca agaccgggac gagcgagaac ttcttcgacg cgtggttcat cggcttcacc
     1921 ccgcaattgc taaccggcat ctggatgggg tacgccgatg gcggccaaac cctcgagtac
     1981 gatctggagt acgcccgaga gctgaacggt ctctacggcg gtatcacccc ggctgagatc
     2041 tggaagacgt acatggagca gatgctcgaa ggtcaaccca tcgagcagtt cgagggcgtg
     2101 gagatgccca cacaggagca gaccacccct gctaacgccg aaacaccggg aaccgagcca
     2161 tccggcgccg ccggtccgag ggccgtcgaa cctctccccg ccggcatcct gggcgaccgc
     2221 cccaccaacg tcgaaccgtc gagcgcgagt ccttccagcg ccgccccttc tagcgtgccc
     2281 ccatcgagtg ccgccccatc gagtgccgcg ccatcgagcg cgagtccttc gagcgctaac
     2341 ccctccagga gg
//
LOCUS       DASTUV010000100         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_202774, whole genome shotgun sequence.
ACCESSION   DASTUV010000100 DASTUV010000000
VERSION     DASTUV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202774"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="upp"
                     /locus_tag="VFT03_02090"
     CDS             <1..591
                     /gene="upp"
                     /locus_tag="VFT03_02090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU4846962.1"
                     /translation="RNKLAVLRDERTPTPEFRQAMKELALIMVAEATRDMPTRPVRIR
                     TPLAETEVEEISGPVCLVPVLRAGLGMLDGALALLPQATVGFMGLFRDEETAQPIEYY
                     VNLPRNLEDYLVLVLDPMLATGGSLSATLTKLKDEGATWISCLHAVAAVPGVERLSRE
                     HPDVRLYAAAVDPELDERSFIVPGLGDAGDRLYGTL"
     gene            578..1075
                     /locus_tag="VFT03_02095"
     CDS             578..1075
                     /locus_tag="VFT03_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU4846963.1"
                     /translation="MEPFERADVDTSEEHAALKRARPSWDEYFMRIAHEVATRSTCPR
                     LAVGAVVVHDKRILTTGYNGSPSGMPHCDDVGCLIRIVDGRESCQRTLHAEQNAIIQA
                     AYHGVSVQGASIYCTHQPCLMCVKMIMNAGIEEVRYAGGYPDPLAMELAAEGDLKMIR
                     YSLEP"
     gene            complement(1083..1724)
                     /locus_tag="VFT03_02100"
     CDS             complement(1083..1724)
                     /locus_tag="VFT03_02100"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4846964.1"
                     /translation="MTVGRTPRRRRRPEEAERAILAAARSFLEEHPFRELTVERVMAR
                     TGLSRPAFYAYFRDSYDVVTRMLEGIGGLLFAVDRRWLTGVEGGREEAREILAEALRG
                     GAETFVRHGRVLRAIADAASQDPKVEEVYRFGLIERLIEAVAGRIERDVEAGLSPKGL
                     DPEETARALVLMTERYLLDAFGRPDGRPSRAKTAAVVGTLEAVWVRTLYGFEA"
     gene            2030..2257
                     /locus_tag="VFT03_02105"
     CDS             2030..2257
                     /locus_tag="VFT03_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU4846965.1"
                     /translation="MPDTNSQRNAEGGGYARFFSLGFALLAIIGGPTVAGFFVDRWLG
                     TLPLFMLVGLVLGFVASLFYVYRAIKNLDDT"
     gene            2254..2694
                     /locus_tag="VFT03_02110"
     CDS             2254..2694
                     /locus_tag="VFT03_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846966.1"
                     /translation="MSEHWRLALRYSAWLLGAAALVSGALFFLYGGGHAWSFFYGAGA
                     AVLSFVSTALTISLFSGRSMAAGVMIGAGSFVVRLAFAAAVLGIPAYLGLWPVVPMLV
                     GFAGIYVAENVAATVRVARTRNEPSAKRKARGSAREEAGRRVGN"
     gene            2694..3488
                     /gene="atpB"
                     /locus_tag="VFT03_02115"
     CDS             2694..3488
                     /gene="atpB"
                     /locus_tag="VFT03_02115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU4846967.1"
                     /translation="MGNLALIQQLTQEEIRKQILHTWESAEHSWVVPIHLGPIDISIN
                     KSVWFLFIGAAVTFLILFVGARYLKDRPGMYQVIVEELYGFGRNHLGGQVAEEGKKWF
                     PYTLSLFVFILVLNIIGLFPGSYPVTSSISFTLTLALLTFILTQFEGVRRNGLFRYLK
                     SLVPPGLPAKPIMVPFMAFIELVQEFSKPLTLGMRLYANILAGHLIIFVFLSFILYFG
                     TFMALISVPAAVIFFAFEIFVAVLQAYIFAILTQVYIELAMFREDH"
     gene            3535..3798
                     /gene="atpE"
                     /locus_tag="VFT03_02120"
     CDS             3535..3798
                     /gene="atpE"
                     /locus_tag="VFT03_02120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU4846968.1"
                     /translation="MEILPMLALLQGSTEGFRYIGTGVAAGTAVIGSGIGMGYLIGTT
                     IAAIHRQPEAFDQTRTLMFLGIGLVEAQALFGLVFALLVAFVL"
     gene            3821..4336
                     /gene="atpF"
                     /locus_tag="VFT03_02125"
     CDS             3821..4336
                     /gene="atpF"
                     /locus_tag="VFT03_02125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU4846969.1"
                     /translation="MNPIFDLFNTSLIFWEIITFAILLFLLYRYVYPPIRDQIQRRQS
                     EIEQAIDEAQKTRSEARELLAEYRRQIEEARGEARQILDEARKQGEAARERAKREARE
                     EADRIIQRAREEIGRERDAALRELRREVADMVIVATERVIGHELDREEHERLISEALD
                     SLEAEVAGEAK"
     gene            4338..4877
                     /gene="atpH"
                     /locus_tag="VFT03_02130"
     CDS             4338..4877
                     /gene="atpH"
                     /locus_tag="VFT03_02130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU4846970.1"
                     /translation="MSAVSTYAEALFEAARERDELEEVLEELQEFETALEESEELRLF
                     FYGSQIPEREKRRAIDALTEGMSLTTRNFLKVLSDNGREEIFEEILLRYEELVKEYLG
                     KVEVAVTTAVELSEDQLDRMKERLGRSLEGREVILQTSVDPNILGGAVFRYGGRMIDS
                     SVRGRLEGLREEMLERGVI"
     gene            4877..>5813
                     /gene="atpA"
                     /locus_tag="VFT03_02135"
     CDS             4877..>5813
                     /gene="atpA"
                     /locus_tag="VFT03_02135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU4846971.1"
                     /translation="MGRLRPEEVSSILKSAITDYENRVRTEEVGQVLEVGDGIARVHG
                     LQNAMYQEMLEFEDARGDKVIGLALNLEEDNVGVIIAGDDTHIQEGNLVRRTERLASV
                     PVGEGVLGRVIDALGFPMDGKGEIEAEENLPVEIVAPGIIKRQPVKQPLQTGIKAIDA
                     MIPIGRGQRELIIGDRKTGKSAIAIDTIISQADQDVKCIYVAIGQKDATVAGVMADLE
                     EAGALDYTTVINASASQSATLQYLAPYAGCAIGEFFMNRGEDALLIYDDLSKHAVAYR
                     QMMLLLRRPPGREAYPGDVFYLHSRLLERAAKLSED"
BASE COUNT         1059 a         1868 c         1863 g         1023 t
ORIGIN      
        1 cggaacaagc tcgccgtcct tcgcgacgag cggacgccca ccccggagtt caggcaggcc
       61 atgaaggagc tcgcgctcat catggtcgcc gaggccaccc gcgacatgcc cacccgtccg
      121 gtgcggatac gaaccccgct ggccgagacg gaggtggagg agatctccgg tcccgtttgt
      181 ctcgtccccg tcctgcgcgc cggcctcggg atgctcgatg gcgcactcgc cctgttgcca
      241 caggccactg tcgggttcat ggggctcttc agggacgagg agacggccca gccgatcgag
      301 tactatgtga acctcccccg gaacctcgag gactacctgg tcctggtact cgacccgatg
      361 ctcgctaccg gcggcagcct ctcggcgacc ctgacgaagc tgaaggacga aggggcgacc
      421 tggatctcct gcctccacgc cgtcgccgcc gtgcccggcg tcgagcgcct cagccgggag
      481 caccccgacg tcaggctcta cgccgccgcc gtagatcccg agctcgacga gcggtcgttt
      541 atcgtgccgg ggctcggcga cgcgggagac aggttgtatg gaaccctttg agagagccga
      601 cgtggacacc tccgaagagc acgccgcgct gaagcgcgcc cgtccctcgt gggacgagta
      661 cttcatgcgc atagcgcacg aggtcgccac gcgctcgacg tgcccgcgtc tcgcggtcgg
      721 cgcggtggtc gtgcatgaca agcgcattct taccaccggc tataacggga gcccatcggg
      781 tatgccccac tgcgacgacg tgggatgcct catccggatc gtggacggtc gcgagagttg
      841 ccagcgcacc ctgcacgcag agcagaacgc cattatccag gccgcttacc atggcgtctc
      901 ggtccagggc gcgtctatct actgcaccca ccagccgtgc ctgatgtgcg tgaagatgat
      961 catgaacgcc ggcatcgagg aggtccgcta cgccggtggc taccccgatc ccctcgctat
     1021 ggagctcgcc gccgaggggg acctgaagat gatccggtac tcattggagc cctgaacaac
     1081 ccctacgcct cgaatccgta gagggtcctg acccacaccg cttctagcgt tccgaccaca
     1141 gcagccgtct tcgcgcgcga cgggcgccca tccggccgcc cgaaggcgtc gagcaggtac
     1201 cgctcggtca tgagcaccag agcccgcgcc gtctcctcag ggtccagacc tttcggtgat
     1261 aaacccgcct cgacgtcgcg ctctatgcgg ccagctaccg cctcgatgag gcgctcgatc
     1321 agaccgaacc tgtagacctc ctcgaccttc gggtcctggc tcgccgcatc cgctatcgcc
     1381 cgcaataccc gcccgtgcct gacaaatgtc tccgcacccc cgcgcaaggc ctccgctaaa
     1441 atctcgcgcg cctcctcccg ccccccttcg acccccgtga gccagcgccg gtccaccgcg
     1501 aacagcagcc caccaatacc ctcgagcata cgcgtaacca cgtcgtagct gtcccggaag
     1561 tacgcgtaaa acgccggccg cgatagcccc gtgcgcgcca tgacccgctc cacagtcaat
     1621 tcccggaacg gatgctcctc tagaaaagac cgagccgcag cgagtatcgc ccgctccgct
     1681 tcttccggcc gccgcctgcg ccgcggcgta cgccccaccg tcatctcgcc tcccgcatgc
     1741 acacaactgt actagcttat tgacaccgtg tctattgaca tcgcgtcaat aaagaagtag
     1801 acttgggttt acaagagttc ctaacgaagg agagcgtgaa ggctcgtgga gatcttgcgg
     1861 tcttttcccg ggatgcaagg tgcgtagtgt gctgcgaagt tgggtaaaga gcagcgtgga
     1921 tgaacaccaa gagtggggag cgagcgctcg cgccagggcc ttccttgcga aggtggcgag
     1981 agcttgaaac tttttgcaac actttgcgcg ttagggtatg atcttgtcga tgcccgacac
     2041 caattcccag aggaacgccg agggtggagg atacgcccgt ttcttcagtt tagggttcgc
     2101 cttactcgct attatcggcg ggcccactgt ggcggggttc ttcgtggaca ggtggcttgg
     2161 gacgctgccg ctcttcatgc ttgttgggct ggtgctcggg ttcgtcgcga gcctgttcta
     2221 cgtgtaccgg gcgatcaaga atctggacga cacgtgagcg agcactggcg gctggcgctc
     2281 aggtactctg cgtggctgct cggcgcagct gccctggttt cgggcgcgct gttcttcctt
     2341 tatggtggcg gccacgcgtg gtctttcttc tacggcgcgg gggcggcggt cttgagcttc
     2401 gtctcgacgg cgctcacgat ctcgctcttt tccgggcgct ccatggcggc gggcgtgatg
     2461 atcggggctg gatctttcgt ggtgaggctg gccttcgccg cggcggtact cggcatcccg
     2521 gcgtacctcg gcctctggcc cgtggtgccc atgctggtcg gatttgcggg gatttacgtg
     2581 gccgagaacg tggccgccac ggtgagagtg gcgagaacta gaaacgagcc gagcgccaag
     2641 cgcaaggcgc ggggatcggc tcgcgaggaa gcaggacgga gggtggggaa ttgatgggaa
     2701 acctggcgct gatacagcag ttgacccagg aggagatacg gaagcagatc ctccacacct
     2761 gggagagtgc ggagcactcc tgggtagtcc cgatacacct cgggcccatc gacatctcca
     2821 tcaacaagtc cgtctggttc ctgttcatcg gggcggcggt gacgttcctg atcctgttcg
     2881 tgggcgcgag gtacctcaag gaccggcccg gcatgtacca ggtcatcgtg gaggagctct
     2941 acggcttcgg acgcaaccac ctcggggggc aggtcgccga ggagggcaag aagtggttcc
     3001 cttacacgct ctcgctcttc gtcttcatac tggtgctcaa catcatcggg ctcttcccgg
     3061 gcagctaccc cgtgacgagc agcatctcct tcacgctgac gctcgcgctg ctgaccttta
     3121 tcctgacgca gttcgagggc gtaaggcgca acgggttgtt caggtatctc aagagcctcg
     3181 tgccaccagg cttgccggcc aagccgatca tggtcccgtt catggcgttc atcgagctcg
     3241 tgcaggagtt ctccaagccg ctcacgctcg gaatgcggct atacgccaac atactcgccg
     3301 ggcacctgat catcttcgtc ttcctcagct tcatcctgta cttcgggacg ttcatggccc
     3361 tgatctccgt ccccgccgcg gttatcttct tcgcgttcga gatcttcgtc gcggtccttc
     3421 aggcttacat tttcgctata ctcacgcagg tttacatcga gctggcaatg ttccgcgagg
     3481 atcactagtc gcggtccagg gattaagaga aagagcagag aggagcagca acgcatggag
     3541 atattgccga tgttggcgct actgcaaggg agcaccgagg ggttcaggta tatcgggacc
     3601 ggcgtcgccg ccggcacggc ggtcatcggg tcgggcatcg gtatgggcta cctcatcggg
     3661 acgaccatcg ccgccatcca ccgccagccc gaggcgttcg accagacccg caccctcatg
     3721 ttcctcggca tcgggctggt ggaggcgcag gcgctcttcg gcctcgtctt cgccctgctc
     3781 gttgcattcg tcctctaggc ggaggtaaag aggagagcgc atgaacccga tcttcgacct
     3841 cttcaacacg agcctgatct tctgggagat catcaccttc gcgatcctcc tgttcctgct
     3901 ctaccgctac gtatacccgc cgatacgcga ccagatccag cgccgccagt ccgagataga
     3961 gcaggccata gacgaggcgc agaaaacccg cagcgaggcg cgcgagctcc tcgccgagta
     4021 ccggcgccag atcgaagagg ccaggggcga ggcgcgccag atcctggacg aggcccgcaa
     4081 gcagggcgag gccgcgcgcg agcgagccaa gcgcgaggcg cgcgaggagg cagaccgtat
     4141 catccagcgc gcccgcgagg agatagggcg cgagcgggac gcggccctgc gcgagctgcg
     4201 ccgcgaggtc gctgacatgg ttatagtggc taccgagcgg gtcatcgggc acgagctcga
     4261 ccgcgaggag cacgagcgcc tgatctccga ggccctcgac agcctcgagg ccgaggtcgc
     4321 cggagaggcg aagtagcttg agcgctgtct cgacctacgc cgaagccctc ttcgaggccg
     4381 cccgcgagcg cgacgagctc gaggaggttc tcgaagagct tcaggagttc gagaccgcgt
     4441 tggaggagag cgaggagttg cggctcttct tctacgggag ccagatcccc gagcgcgaga
     4501 agcgccgggc gatagacgcc ttgaccgagg ggatgtccct cacgaccagg aactttctca
     4561 aggtcctctc ggacaacggg cgggaggaga tcttcgagga gatcttgctt cgctacgagg
     4621 agctcgtgaa ggagtacctt ggcaaggtgg aggtcgcggt cacgacggca gtggagcttt
     4681 ccgaggacca gctggaccgc atgaaggaac ggctcgggag gtcgctcgaa ggccgtgagg
     4741 taatattaca gacgagcgtc gacccgaaca tcctgggcgg cgcggtcttt cggtacggag
     4801 gacgcatgat tgacagcagt gtccgggggc ggctagaggg cctccgggag gagatgctcg
     4861 agaggggtgt aatctagtgg gcaggctcag gccggaggaa gtctcctcca tactcaagag
     4921 cgcgataacg gactacgaga acagggtccg caccgaggag gtcgggcagg tactggaggt
     4981 cggggacggt atagcgcgcg tccacggcct ccagaacgcg atgtaccagg agatgctcga
     5041 gttcgaggac gcgcgcggcg acaaggtcat cggcctcgcc ctcaacctcg aagaggacaa
     5101 cgtcggcgtc atcatcgccg gcgacgacac gcacatccag gagggcaacc tggtgcggcg
     5161 caccgagcgc ctcgcgagcg tgccggtcgg cgagggcgtg ttggggaggg tcatagacgc
     5221 cctcggcttc ccgatggacg ggaagggcga gatcgaggcc gaggagaacc tcccggtcga
     5281 gatcgtcgcg cccggcatca tcaagcgcca gcccgtcaag cagcctctcc agacgggcat
     5341 caaggcaata gacgccatga tccccatcgg ccgcggacag cgcgagctca taatcggcga
     5401 taggaagacg ggcaagtcgg ctatcgcgat agacacgatc ataagccagg ccgaccagga
     5461 cgtgaagtgc atctacgtcg ccatcggcca gaaggacgcc accgtcgcgg gcgtgatggc
     5521 cgacctcgag gaggccggcg cgctggacta caccaccgtc atcaacgcct cggcttccca
     5581 gtcggcgacg ctccagtacc tcgcccctta cgctggctgc gccatcggcg agttcttcat
     5641 gaaccgcggc gaggacgcgc tcctcatcta cgacgacctc tccaagcacg ccgtcgccta
     5701 ccgacagatg atgctcttgc tcaggcgtcc gcccgggcgc gaggcttatc cgggcgatgt
     5761 cttctacctc cactcgcgcc tgctggagag ggcggcgaag ctctccgaag atg
//
LOCUS       DASTUV010000101         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1704166, whole genome shotgun sequence.
ACCESSION   DASTUV010000101 DASTUV010000000
VERSION     DASTUV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704166"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1379
                     /locus_tag="VFT03_02140"
     CDS             189..1379
                     /locus_tag="VFT03_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU4846972.1"
                     /translation="MVEDRVQAEIMACVRAEAGPISRARLIEALNSSRGKISAEVVRL
                     IEKGLLAENGLAASEGGRRSSLLVIPPSAGLIAAVDIGATSIDVALTTLGNELVARRD
                     EPADVREGPLPVLDRVKTLISELIDEQAASPRDVLAIGVGVPGPVEQASGLLTVPPIM
                     PGWDRFPIRGAFAGEYAAPVFVDNDVNVMVLGEHWGGVAKGVADVVFVKIGTGIGSGI
                     IVGGRLHRGAQGCAGDFGHICVDPDGPLCTCGNTGCLEAMAAAPAIVLQAERCAREGE
                     SLSLIALLQEKGELTMKDVGEAARRGDYCAQTIIRRSGRLIGQTLASAVNVLNPSMIV
                     IGGGVSRVGHALLAEVRSAVYQRSLPLATRNLPIVMSELDDVAGVVGASVMAAGEVLS
                     FAGR"
     gene            1522..2613
                     /locus_tag="VFT03_02145"
     CDS             1522..2613
                     /locus_tag="VFT03_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4846973.1"
                     /translation="MLERRTIGRALALVVCSVAVVFAAACGGGEQGGNGGGGGNQGPF
                     TIGVSNGFISSEWRTQMIDDLQRVNEEYKQEGLTEDLVIESADVDPQGQIQQMRNLIN
                     RGVDGIIVNPNDATALDQVIGQAEQAGIPVIAVDQEIDAPGVTNVVIDQGEWASMGAE
                     WLVKELDGEGDVVVINGIAGHPANEARYDAVKEVFAKNPGIEVVQETNADWDQATAQQ
                     EMSNILASQPNIDGVWTQDGMARGVLQAVQAANPGEYPVISGEARSGYMRLWQEVRQQ
                     NPDFHSYGPINPPGVGASGLRVMVELLQGKEIKQSELEGEAGNTLYVPIPGVVTDQNL
                     DQELQKIDVEGEYVLDGIISRKQAQSYFE"
     gene            2624..>2906
                     /locus_tag="VFT03_02150"
     CDS             2624..>2906
                     /locus_tag="VFT03_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4846974.1"
                     /translation="MAEVSLSAQHLVKRYGGVVALADGSLDVESGEVVALMGANGSGK
                     STLGKIITGAVVPDGGLLLLDGEEARFSSPQAARRAGISAVYQELSLVPD"
BASE COUNT          580 a          814 c         1039 g          473 t
ORIGIN      
        1 gggcagcaag gctctgaggt cgtgtgttgg ccagctcact gcaaaagagc gacgtagaga
       61 agcaaatatt tacacaattt tgacaaaagt cgttgacgct aaagcgaaag cctgtctatc
      121 attcgtaaca agggaaggag aaggtgcgcg gcgagcgtgt tagaaaggta gggcctccac
      181 gctatccctt ggtagaagat cgggtccaag ccgagatcat ggcctgtgtc agggccgagg
      241 cggggccaat ctcgcgggcc cggctgatcg aagcgctgaa ctccagccgc ggcaagatat
      301 cggccgaggt cgtgcggctc atcgagaagg ggctgttggc cgagaatggc ctcgcggcat
      361 ctgagggagg gcgccgttcg tccctgctag tcatcccacc ctccgctggc ctcatcgcag
      421 cggtggacat cggagcaacc tccatcgacg tggccctgac gacactcgga aatgagctgg
      481 ttgccaggcg ggacgaacct gccgatgtaa gggaggggcc actgcctgtt ctcgatcggg
      541 taaagactct catttcggaa cttatcgacg agcaagccgc gagcccgcgg gacgtactcg
      601 caatcggggt gggcgtaccc ggtcctgtcg agcaggcctc tggtctgtta acggtgcccc
      661 cgatcatgcc gggttgggac cgcttcccga tacgcggcgc gtttgctgga gagtatgccg
      721 ctcccgtctt cgtcgacaac gacgtgaacg tgatggtgct cggcgagcac tggggcgggg
      781 tggcgaaggg cgtcgccgac gtggtcttcg tcaagatcgg cacgggtata ggtagcggga
      841 tcatcgttgg cggccgcctt caccgtggtg cccagggctg cgccggggac tttggccata
      901 tctgcgtgga tcctgatggc cccctctgca cttgcggaaa caccggctgc ctggaggcga
      961 tggcggccgc accggcgatc gtgctgcaag cggaaaggtg cgccagggaa ggagagtcgc
     1021 tgtccctcat agccctcttg caggagaagg gcgagctcac catgaaagat gtcggagagg
     1081 ccgcgaggcg tggcgactac tgcgcccaga caatcatccg caggtccggg aggttgatcg
     1141 gccagacgct ggcctcggcc gtcaacgtgc tcaacccttc gatgatcgtg atcggcggcg
     1201 gggtttctcg cgtgggtcac gcgcttctgg ccgaggtgcg aagcgccgtc taccagcggt
     1261 cgctgccact cgcaactcgc aacctcccga ttgtcatgag tgaactcgat gacgtggcgg
     1321 gcgtcgtagg cgcgagcgtg atggctgcgg gggaggtgct ctcgtttgcg ggccggtagc
     1381 aaacaccact ctccattggg gcggtcggag cgaggagagg agcatgaaag cggcagagcc
     1441 aagagcttga aaaagcgatg agtagcggag caaaaagcgc cgggtaacat cttcggcagc
     1501 gtgcaggaag gagaggacag gatgctcgaa cggaggacga tcggcagggc cctggcgctg
     1561 gtggtgtgct cggtggcggt ggtattcgcg gccgcctgtg gcggtggcga acagggcggg
     1621 aacggtgggg gtgggggcaa ccagggaccg ttcaccatcg gggtctccaa cggcttcatc
     1681 agcagcgagt ggcggacgca gatgatcgac gacctgcaga gggtcaacga agagtacaag
     1741 caggaagggc tcaccgagga tctggtcata gaaagcgcgg acgtcgatcc gcagggccag
     1801 atccagcaga tgagaaacct cattaaccgc ggggtggacg ggataatcgt caaccccaac
     1861 gatgctaccg cgctcgacca ggtgatcggt caggccgagc aggccgggat cccggtgatc
     1921 gcggtggacc aggagatcga cgccccgggc gtcacgaacg tcgtgatcga ccagggcgaa
     1981 tgggcgagca tgggcgccga gtggctggtg aaggagctcg atggcgaggg tgatgtggtg
     2041 gtgatcaacg gcatcgctgg ccacccggcc aacgaggcgc gctatgacgc tgtgaaggag
     2101 gtcttcgcca agaacccggg tatcgaggtg gtccaggaga cgaacgcgga ctgggaccag
     2161 gcgacggccc agcaggagat gtcgaacatc ctcgcctcgc agccgaacat cgatggcgtc
     2221 tggacgcaag acggcatggc gcgcggcgtg ctgcaggccg tgcaggccgc caaccctggt
     2281 gagtacccag tgatctcggg cgaggcgaga tccggctaca tgaggctctg gcaagaagtg
     2341 cgccagcaga acccggactt ccattcctac gggccgatca acccgcccgg cgtcggcgcg
     2401 agcggcttgc gggtgatggt agagcttttg cagggtaagg agatcaagca gagcgagctc
     2461 gaaggcgaag cgggcaatac cctgtacgtg ccaataccag gggtggtgac cgaccagaac
     2521 ctcgatcagg agctgcagaa gatcgacgtc gagggcgagt acgtactcga cggcatcatc
     2581 agccgcaaac aggcgcagtc ctacttcgag taggcgggcg gcgatggctg aagtctccct
     2641 ctcggcccaa cacctcgtca agcgctacgg cggtgtagtg gcgcttgccg acggcagctt
     2701 ggacgtggag tccggcgagg tggtggcgct gatgggggcc aacggtagcg gcaagagtac
     2761 gctcggcaaa atcatcacgg gagcagtcgt ccccgacgga gggctactgc tcctggacgg
     2821 cgaggaggcg cgcttctcct cgccccaggc ggcccgccgg gcagggatct ctgccgtcta
     2881 ccaggagctc agtctagtcc cggaca
//
LOCUS       DASTUV010000102         4918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1669438, whole genome shotgun sequence.
ACCESSION   DASTUV010000102 DASTUV010000000
VERSION     DASTUV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669438"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFT03_02155"
     CDS             complement(<1..688)
                     /locus_tag="VFT03_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4846975.1"
                     /translation="MTRASLCVFALAVYLVVCYVVAARLSAPVRRPVERTPAYFGLRY
                     REVEVRSSDGLRLSGWWVDGAGSSRAAVLVHGWGADRSSRHVLETAAVYEEAGYDVLV
                     LDPRGHGGSEGDRVTLGYREVRDVRGALSWLEKRGFDPERVVLHGWSMGGAAVIQAAP
                     GTGVAAVVEESAYADLPPLLRERLPEVSGLPAFFTPGIFLVGRLFLGIDPWAVRPVEE
                     AGQLSREGVPF"
     gene            complement(720..1733)
                     /locus_tag="VFT03_02160"
     CDS             complement(720..1733)
                     /locus_tag="VFT03_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU4846976.1"
                     /translation="MRVYSDPASERHVNQLASVESPARFPAALAGVERAERDGVPVEW
                     LPCNPADEDDLARVHHPAYLDHLKSLSAQGAGYLSLDTALNEHSWEAASLASGAAVAA
                     VESALSGEPAFAVARPPGHHAGRATGMGFCLLNHVAVGAGHARSRGVGRVAILDWDVH
                     HGNGTQDIFYEDGEVLYLSAHQSPFYPGTGWADEVGEGEGKGLTVNVPLPAGSGEGVY
                     AAAFAGVFLPVLREFRPALILISAGYDAHRADPLGGMALEAASFGRFAAALASLAREI
                     GTPPPALVLEGGYNLGALTDGVAATIRGIEEAREAGREYAGGVRPVEAARKTLAPYWE
                     SLR"
     gene            complement(1808..2377)
                     /locus_tag="VFT03_02165"
     CDS             complement(1808..2377)
                     /locus_tag="VFT03_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU4846977.1"
                     /translation="MFGPKGMKGFFGRGPWRGHGFDPRRGRVFGKGDLKYVILDLIKD
                     QPAHGYEVMRALEGRFRGFYSPSPGSVYPTLQMLEDLGYVTSTQRDGKKVYEITAEGR
                     RFLDENRRSVEDIWGRVGGGRWDPEVAAGLHEMKHEMVGLGKLFGREMHEGRLDREKL
                     DRVREVVARAAREIEHILDDREEPGTTRV"
     gene            complement(2406..2936)
                     /locus_tag="VFT03_02170"
     CDS             complement(2406..2936)
                     /locus_tag="VFT03_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846978.1"
                     /translation="MLSRYIVIYCGNAGERNGGEMYGWGRMGYWGRGRGMAPRPGGGP
                     PVVLVVGLVVGGLFLFSMAGAFDFFPFFFFFWLIPFFLIPVIGLSARSVAGLFESRAR
                     RPIDEGKREKEVLEALARHGELTPARVALETSLSVAEAEEVLSRLAKDGHIEVRAREG
                     RLGYALWDTDRRELTA"
     gene            complement(3089..3547)
                     /locus_tag="VFT03_02175"
     CDS             complement(3089..3547)
                     /locus_tag="VFT03_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846979.1"
                     /translation="MRTTIQAGQGRVERRQRAVAEIQILLADVYRGFIAWASLYGDGH
                     YEQEQRERVPALIDRLSNGYLARSMWLEPGARKKVEDFIEKSEDLYSRFCADIEERGY
                     TKKVKKSMANRVSRQLRSARKVAESNLQDDPDDSRSRWPARLRLSARSGL"
     gene            complement(3650..4342)
                     /locus_tag="VFT03_02180"
     CDS             complement(3650..4342)
                     /locus_tag="VFT03_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4846980.1"
                     /translation="MTDEPKRDAERDAQLPVRVVIAEDSGLLREGLSRLLSDAGFEVV
                     AAVGDGARLVSAVEEHRPDVAVVDVRMPPDYRDEGLKAAIEARGRVPGLPVLVLSQYV
                     EERYGAELLESGARGTGYLLKDRVADVPEFVAAVRDVSSGGTVLDPEVVEQLLSRRRR
                     DEGLSSLTPREREVLALMAEGRSNHAIAGTLFVTDGAVEKHVKSIFGKLGLSQTGTDH
                     RRVLAVLAYLRA"
     gene            complement(4339..>4918)
                     /locus_tag="VFT03_02185"
     CDS             complement(4339..>4918)
                     /locus_tag="VFT03_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4846981.1"
                     /translation="DLHDGAQQRLVSLAMDLGMAKEKLDKDPEAVRGLVEEAHGEARR
                     ALSEIRDLVRGIHPAILTDRGLDPALSALAGRSRIPVAVDVRLEDRLPEAVETTAYFV
                     VAEALSNAAKHSGASEAGVAVWHERAPEDSLVVEVTDDGAGGASPVAGGGLSGLRDRL
                     SALDGRLLVRSPEGGPTLVRAEIPLGTTHRKP"
BASE COUNT          778 a         1798 c         1563 g          779 t
ORIGIN      
        1 tgaacggtac gccctcgcga gagagttgcc ctgcctcctc cacgggacgc acggcccagg
       61 ggtctatgcc caggaagagc ctgccaacga ggaagatccc cggagtgaag aaggctggca
      121 ggccgctgac ctcggggagc cgctcccgca gcagcggtgg caggtccgcg taggccgact
      181 cctccacgac cgcggccacg ccggtcccag gagcggcctg gatgaccgcc gcgccgccca
      241 tcgaccagcc gtggagcacc acccgttcgg ggtcgaaacc ccgcttctcg agccaggaca
      301 gcgctccccg gacgtcgcgc acctcgcggt agccgagggt gacccgatct ccctccgacc
      361 cgccgtggcc tcgcgggtcg agcaccagga cgtcgtaccc tgcctcctcg tagacggcgg
      421 cggtctcgag cacgtggcgg ctagacctgt ccgcacccca accgtgaacg agcacggcgg
      481 cccgcgacga gcccgcgccg tccacccacc agccgctcag gcgcagcccg tcgctgctcc
      541 gaacctcgac ctccctgtag cgtaggccga agtaggcggg cgtccgctcg accggcctcc
      601 tgaccggcgc ggagagccgg gccgctacca cgtagcagac taccaggtaa accgcgaggg
      661 cgaagacaca gagagacgcc cgcgtcatcg cccaactcct acgggtggtc caccaggggc
      721 taccggaggc tctcccagta cggcgccagg gtcttccggg cggcctcgac gggtcttacg
      781 ccgcccgcgt actcccggcc ggcctcccgt gcctcctcga tcccccggat ggtcgccgcg
      841 acgccatcgg tgagggcgcc caggttgtag ccaccctcga ggacgagcgc gggcggcggc
      901 gtccctatct cgcgggccag ggaggcgagc gcggcggcga agcgtccgaa ggaggcggcc
      961 tccagcgcca tgcctcccag aggatcggcc ctgtgggcgt cgtagccagc agagatcagg
     1021 atcagagccg gacggaactc gcgcagcacg gggaggaaga ccccggcgaa cgccgccgcg
     1081 tagacgccct cccccgagcc cgcgggcagc ggtacgttga ctgtcaggcc ctttccctcg
     1141 ccctccccta cctcgtcagc ccaaccggtc cctgggtaga aggggctctg gtgcgcagag
     1201 aggtagagca cctccccatc ctcgtagaag atgtcctggg tgccgttgcc gtggtggacg
     1261 tcccagtcga gaatggctac cctccctacc ccgcgcgagc gcgcgtggcc cgcgccgacc
     1321 gcaacgtggt ttagcaggca gaaccccatc cccgtcgcgc gtccggcgtg gtggcccgga
     1381 gggcgcgcca ccgcgaaggc cggctccccc gagagcgcgc tctcgaccgc tgcgactgcg
     1441 gccccgctcg ctagagaagc cgcctcccag gagtgctcgt tgagggccgt gtccaggctg
     1501 aggtagcccg cgccctgagc ggagaggctc ttgagatggt cgaggtacgc ggggtggtga
     1561 accctggcga ggtcgtcttc gtccgctggg ttacagggta accactccac aggtacccca
     1621 tcccgctctg ctctctccac gccggctagc gccgccggga accgcgcggg ggactccacg
     1681 gaggcaagct ggttgacgtg tcgctccgag gcgggatcac tgtagacgcg caagggtccc
     1741 ttccgtcatt catccacaac ccactgcggc ccattatcgt ggtcgcgccc cacctggggc
     1801 aagcgcttta gacgcgcgtc gtccccggct cctccctgtc gtcgaggata tgctctatct
     1861 cccgcgcggc gcgggcgacg acctcgcgga cgcggtcgag cttctctctg tcaagccgcc
     1921 cctcgtgcat ctcgcgtccg aagagcttgc cgaggcccac catctcgtgc ttcatctcgt
     1981 gcagccccgc cgcgacttcg gggtcccagc gccccccgcc tacccggccc cagatgtcct
     2041 cgacggaacg tctgttctca tcgaggaacc tgcgcccctc ggctgtgatc tcatagacct
     2101 tcttgccgtc ccgctgcgtc gaggtcacgt aaccaaggtc ctcgagcatc tgcagcgtcg
     2161 ggtagaccga gccggggctc ggagagtaga acccgcggaa gcgcccctcg agcgcccgca
     2221 tcacctcgta accgtgagcg ggctggtcct tgatcaggtc caggatcacg tacttcagat
     2281 cccccttccc aaacacgcgc ccccgcctcg ggtcgaaccc gtgcccccgc cacggcccac
     2341 ggccgaagaa acccttcatc ccttttggcc cgaacatgat ttcgcctcct gttttcctgc
     2401 cgccttcacg ctgtcagctc ccgcctgtcc gtatcccaca gcgcgtaccc gagcctcccc
     2461 tcgcgggcgc gcacctctat gtgcccgtcc ttcgcaagcc gcgagagcac ctcctcagcc
     2521 tctgccacgc tgagcgaggt ctcgagcgcc accctggcgg gcgtgagctc cccgtgccgc
     2581 gcgagcgcct ccagaacctc cttctccctc ttcccctcat caattggcct ccgcgcccgc
     2641 gactcgaaga ggccagccac actcctcgca gagagcccga tcaccggtat caaaaagaac
     2701 gggatcagcc agaagaaaaa gaagaacggg aagaagtcga acgcgcccgc catcgagaag
     2761 aggaatagcc ccccaaccac aaggcccacc accagcacta ccggcggacc accgcccgga
     2821 cgcggcgcca tccctcgccc cctaccccag tagcccatcc tgccccagcc gtacatctcg
     2881 cctccgttcc tctctccagc gttgccgcag tatatcacga tatatcgtga tagcacgcct
     2941 tccttcggga ggcttttcag ctagcgcggt tcatcgctcc gcagcgcgcc agacagggtt
     3001 actgtcgcaa aggcgtgtcg taatgcgtga cctggcaaga gcccaacgcc agagaggaag
     3061 ctgaaatgct gagaagcgag ggcctttact aaaggcccga gcgtgctgac aagcgaaggc
     3121 gcgcgggcca gcgggagcgc gagtcgtcgg ggtcgtcttg caggttggat tcggcgactt
     3181 tcctggcgga cctgagctgt ctgctcacgc ggttggccat gctcttcttg acttttttgg
     3241 tgtagcctcg ctcttcgatg tcggcgcaga acctggagta gaggtcttcg gacttctcga
     3301 tgaagtcttc gaccttcttc cgggcgccgg gctcgagcca catggatctc gcgaggtagc
     3361 cgttggagag tcggtcgatc aacgcgggga cccgctccct ctgctcttgc tcgtagtgac
     3421 cgtccccgta cagcgacgcc caggcgatga agcccctgta gacgtcggcg agcaatatct
     3481 gtatctccgc cacggcgcgc tggcggcgct cgacccggcc ctgcccggcc tgtatggtag
     3541 tacgcacgat ggtccccctc cggactcgtc tctaccccaa acccacaacc tgtgcgggcg
     3601 cagcgtaaac gtaacacaac atgcagtgtc tttgccaatg cgcgaactat caggcccgca
     3661 ggtaagcgag gacggcgagc accctccggt ggtccgtgcc ggtctgggag aggccgagct
     3721 tgccgaagat gctcttgacg tgcttctcga cggcgccgtc ggtgacgaag agcgtcccgg
     3781 cgatagcgtg gttcgagcgt ccttcggcca tgagggccag gacttcgcgc tcacgcgggg
     3841 tgagggatga gagcccctcg tcgcgccggc ggcgggagag gagctgttcg acgacctccg
     3901 ggtcgagcac cgttccaccc gatgagacgt cgcggacagc ggctacgaac tcggggacgt
     3961 cggccacgcg gtccttcagg aggtagcccg tgccgcgggc gccggactcg agcagctcgg
     4021 cgccgtagcg ctcctcgacg tactgcgaga ggaccagcac ggggaggccg gggacgcggc
     4081 cgcgcgcctc gatggcggct ttgagaccct cgtcgcggta gtctggaggc atcctgacgt
     4141 ccacgacggc cacgtcggga cggtgctctt cgacagccga caccagcctc gcgccgtcgc
     4201 cgacggcggc gaccacctcg aagcccgcgt cggacagcag cctggagagg ccctcccgca
     4261 ggagcccgga gtcctcggcg atcacgaccc gtaccggtag ctgcgcgtct cgctccgcgt
     4321 cccgcttcgg ctcgtccgtc acggcttccg gtgggttgtc ccaaggggta tctcggccct
     4381 cacgagggtg ggtccgcctt cgggacttcg caccaggagc cggccgtcga gagcggagag
     4441 ccggtctctg aggcccgaga gcccgccgcc ggctaccggc gaggcgccgc cagcgccgtc
     4501 gtcggtcacc tcgacgacga ggctgtcctc cggggctcgc tcgtgccaca cggccacgcc
     4561 tgcttccgag gcgcccgagt gtttcgcggc gttggagagg gcttctgcta ccacgaagta
     4621 cgctgtcgtc tcgaccgctt cgggcagacg gtcttcgagc ctcacgtcca ccgcgacggg
     4681 gatccgggat ctcccagcca gagcggagag ggccgggtcg aggcccctgt cggtgaggat
     4741 cgccgggtgg atgccccgta cgaggtcccg gatctccgag agggcgcgcc tggcctcgcc
     4801 gtgggcctcc tcgaccagcc cgcgcaccgc ttcggggtct ttatccagct tctccttcgc
     4861 catccccagg tccatcgcca gcgagacgag acgttgctga gcgccgtcgt gcaggtcg
//
LOCUS       DASTUV010000103         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_23962, whole genome shotgun sequence.
ACCESSION   DASTUV010000103 DASTUV010000000
VERSION     DASTUV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23962"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFT03_02190"
     CDS             complement(<1..248)
                     /locus_tag="VFT03_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846982.1"
                     /translation="MSDRFRAGMAGVVGAIVAFGAVELVHGLYSPVPSVFVALAQQVV
                     ERTPGELVTRGIELLGQADIPVLVTSMIVGALVVAGALA"
     gene            774..>2541
                     /gene="ileS"
                     /locus_tag="VFT03_02195"
     CDS             774..>2541
                     /gene="ileS"
                     /locus_tag="VFT03_02195"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU4846983.1"
                     /translation="MAFEKVRPQVDFPALEKEVLGLWEEIGAFQKSVERRPEDRPFVF
                     YEGPPTANGRPGFHHALSRAFKDLIPRYKTMQGYRVERKAGWDTHGLPVEIQVEKELG
                     LSGKDDIERYGVEEFNRLCRESVFRYVEDWRAMTERLGFWIDMEHPYRTLDNSYMESV
                     WWALKELYEKDILYEGYKVSAHCPRDQTSLSAAEVALGYQQYPDPVVDPSVYVRFPLV
                     DQEDTSLLTWTTTPWTLISNAAVAAHPEVDYATAEVDGERLILARDLLDRVLGEGNYE
                     VLETRKGAELEGLRYRRPFDYVPVERAEEHENLWTVVLSEHVTTTEGTGLVHIAPAYG
                     EDDARLGRRYGLPTIHPVKPDGTFDQRVGPYAGQFVKDADTRLVEELMERGLLFRAEE
                     YEHAYPHCWRCGTPLLYYAKRAWYARTTAVLDELLSENEKINWVPEHIKWGRFGDWLK
                     NNIDWALSRERYWGTPLPIWRTEAGDVIVVGSLEELRELAMDPVPDDLHRPYIDTVRI
                     RHPETGEDAHRVPEVLDVWFDSGSMPFAQWAYPYAKGSQESFERHFPADYICEAVDQT
                     RGWFYSLHAISTMLFGRTSFKTC"
BASE COUNT          490 a          841 c          827 g          383 t
ORIGIN      
        1 gccagagcgc cggcgacgac cagagcgccg actatcatcg acgtgaccag aacaggtatg
       61 tcggcctgac cgaggagctc tatgcccctc gtcacgagct cccccggcgt tcgctccacc
      121 acctgctggg cgagcgctac gaacacagat ggaacgggag aatacaggcc gtggactagc
      181 tccacggcgc cgaacgccac gatcgctccg acgacgcccg ccattcccgc tctaaacctg
      241 tcgctcatga cctaatctcc catgtctcgc ccggcgtgct ctcgctattg caacaatacc
      301 atcatcggcc actttcacgg cctctacacc tgctactacg acggcgcggg cggtcccgga
      361 tcaccggcca gcgagcgccg ccttgacacg cgcgccgcca agggctacaa acgctctata
      421 aaaaggcgac gacgggaacg agtaaggctg cacaaccccg aatgagcgag ccgggggcgg
      481 tgcgaagccc ggcacgggac gcggcccgaa gatcctcccc gagccgccga ccgaaagcga
      541 agggccgtgc gatagccagc ccaggcgtta gtaggaaagg ccgggcacgc ccgttacagc
      601 gtgagggtgt cttgtggcac ccgttgagag gtccggggct gtgaggcgcc gggcaagcgg
      661 ggtggtaccg cgagggtctt ccaggctccc gtccctgcgg ctgtagagac gcgctatggt
      721 acgtctctac cgccgcagtg ggcggggctt ttgcgttgag aggtgtgatt ggtatggcgt
      781 tcgaaaaggt aagaccgcag gtagatttcc ccgcgctgga gaaggaggtc ctgggcctct
      841 gggaggagat cggggcgttc cagaagtccg tcgagaggcg gccggaagac aggccgttcg
      901 tcttctacga ggggccgccg acggccaacg gcaggccggg cttccaccac gctctatcta
      961 gggctttcaa ggatctgatc ccgcgctaca agaccatgca gggctaccgc gtcgagcgca
     1021 aggccggctg ggacacccac ggcctccccg tcgagatcca ggtcgagaaa gagctaggcc
     1081 tctcgggcaa ggacgacatc gagcgctacg gcgtcgagga gttcaaccgg ctctgccgcg
     1141 agtccgtctt ccgctacgtc gaggactggc gcgcgatgac cgagcgcctg ggcttctgga
     1201 tagacatgga gcacccctac cgtaccctcg acaactccta catggagagc gtctggtggg
     1261 cccttaagga gctctacgag aaggacatcc tctacgaggg ctacaaggtc agcgcccact
     1321 gcccccgcga ccagacctcc ctctcggccg ccgaggtcgc cctgggctac cagcagtacc
     1381 cggaccccgt ggtcgacccg agcgtatacg taaggtttcc gctggtggac caggaggaca
     1441 ccagcctgct cacctggacc acgaccccgt ggaccctcat ctccaacgcc gccgtcgcgg
     1501 cccaccccga agtggactac gcgaccgccg aggtcgatgg ggagaggctg atcctggcgc
     1561 gtgacctgct cgaccgcgtg ctgggcgagg ggaactacga ggtcctagag acgcgcaagg
     1621 gtgcggagct cgaagggctg aggtatcgac gccccttcga ctacgtgccc gtggagaggg
     1681 ccgaagagca cgagaacctg tggacggtgg tcctcagtga gcacgtcacc acgaccgagg
     1741 gcacgggcct ggtgcacatc gcccctgcct acggcgagga cgacgcccga ctcggacgcc
     1801 gctacggcct ccccacgatc caccccgtaa agccggacgg gacgttcgac cagcgcgtcg
     1861 gaccgtacgc cggacagttc gtgaaggacg cggacacgag gctcgtggag gagctaatgg
     1921 agagaggtct gctcttcagg gccgaggagt acgagcacgc ctatccccac tgctggcggt
     1981 gcggcacgcc gctcctctac tacgccaaaa gggcctggta cgcccggacg accgccgtac
     2041 tcgacgagct gctctcggag aacgagaaga tcaactgggt ccccgagcac ataaagtggg
     2101 ggcgcttcgg agactggctc aagaacaaca tagactgggc cttgagccgc gagcgctact
     2161 ggggcacgcc gctcccgatc tggcgcaccg aggcgggcga tgtcatcgtc gtcggcagct
     2221 tggaggagct cagagagctg gcgatggacc cagtgcctga cgacctgcac cgcccgtaca
     2281 ttgacacggt gcgcatcagg caccccgaga cgggcgagga cgcgcaccgt gtccccgagg
     2341 tcctcgacgt ctggttcgac tcggggagca tgcctttcgc ccagtgggca tacccttacg
     2401 ccaaaggcag ccaggagagc ttcgagcggc acttcccggc cgactacatc tgcgaggccg
     2461 tggaccagac gcgcggctgg ttctactcgc tgcacgccat ctcgacgatg ctcttcggca
     2521 ggacctcgtt caagacgtgc c
//
LOCUS       DASTUV010000104         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1059833, whole genome shotgun sequence.
ACCESSION   DASTUV010000104 DASTUV010000000
VERSION     DASTUV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059833"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFT03_02200"
     CDS             complement(<1..891)
                     /locus_tag="VFT03_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HEU4846984.1"
                     /translation="MKNHLREILAASLAAADPEEAVRRLVRVEGNTVLVGDERFEAER
                     VFVLAAGKAAGAMARAAEELFGERIAGGLIVTRYGHEAASEKLEIIFAGHPEPDEKSV
                     EAARSAEELARSLVEGDLLLALISGGASALLADPAPPIELADLKKLTGALLRSGANIS
                     EINTVRKHVSVLKGGGLVKLAAPAPTLALLLSDVVGDDPSAIASGLTAPDPTTLEETQ
                     RVFRRYGIEPPRSVVEHLRDAEETPKPGDALFEKVASVVCGSGRHAAEAATEKARELG
                     YEPILLSTSVTGDARGIASVY"
     gene            complement(918..2687)
                     /gene="gcl"
                     /locus_tag="VFT03_02205"
     CDS             complement(918..2687)
                     /gene="gcl"
                     /locus_tag="VFT03_02205"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HEU4846985.1"
                     /translation="MPRMNVMDAVVKVMEDEGVEVAFGVPGAAILPLYKALSKSDQIK
                     HYSVRHEEGGTHAADGYTRVTGKVGINIGTSGPAGTNMITGLYTCMADSVPQICITGQ
                     APTSMLHKEAFQAVDIVDIAKPVTKWAVQVKEPSQLVWTFREAFRVAREGRPGPVLID
                     LPLDVTKSGMEVDFDPRRGGRLPFTQPAPNPNAIRQVVEMILEAERPMLMPGGGVIIA
                     DASEELVDVAEYLQIPVTPTYMGKGTIPEDHPLYAGIVGLQTQQRYANALFLESDLVV
                     GIGNRWAERHTGDLDVYRGDRKFIHIDIDPGQLGRVFQPDLAVVSDARLALQEMLDTA
                     RDMTPAREPGEWVDRVDELRSTLLRKMDFDDVPVKPQRAFQEMNEFFDEDAIIVTAIG
                     LYQIFSGQFQKTYKPRHYLCCGQAGPLGWEVPACIGVKLGRPENLVVGVVGDYSFQFL
                     MEEVAVAVQYRVPYVLVMLNNAYMGLIRQAELGYDMNFAVDLGYEGPANEYGIDNVGV
                     MEAMGALGRRVTRPEEIKGALEWAVRASEERRVPALVEIICERETNAAMGLSIDKINE
                     YEPILDGTREMAGQVVGGVPERD"
     gene            complement(2719..3609)
                     /locus_tag="VFT03_02210"
     CDS             complement(2719..3609)
                     /locus_tag="VFT03_02210"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEU4846986.1"
                     /translation="MAERVGFIGLGIMGMPMARNLMEAGYELTVHNRSPEKAEELGKE
                     GAAVAATPREVAENSDVVITMLPDSPQVREVVAGEDGVLEGISEGALLIDMSTISPVV
                     TEDLAEAVKEKGASMLDAPVSGGDVGAIEGTLSIMVGGQERDFQRAKPLLEAMGKTIT
                     HVGPTGAGQVTKAANQVVVALTIEAVSEALVLGSAGGVSAEKILEVLSGGLASNKVME
                     VKREKFLSHTFEPGFRSELHHKDLGIALAAGREYGVVMPVTAIVDQMLLSMKRKGWGG
                     EDHSALLRIIEDLSQHTIGE"
     gene            complement(3622..>3821)
                     /locus_tag="VFT03_02215"
     CDS             complement(3622..>3821)
                     /locus_tag="VFT03_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEU4846987.1"
                     /translation="DSPARGEPGTGEIHYPYVLGVLEELGYDGYVGLEYNPTTERTED
                     SFAWIPEPLRGSDVKVGDLNL"
BASE COUNT          600 a         1331 c         1133 g          757 t
ORIGIN      
        1 atacaccgag gctatcccgc gggcgtcgcc ggtgacgcta gtcgagagca atatcggctc
       61 gtagccgagc tcgcgggcct tctcggttgc ggcctccgcc gcgtgccgcc cgcttccaca
      121 gacgacgctg gctaccttct cgaatagagc gtcgccgggc ttcggcgtct cctccgcgtc
      181 tctcaggtgc tccactacgc tcctcggggg ctctatcccg tagcgtctga acacccgctg
      241 cgtctcctcc agggtggtcg ggtcgggggc tgtgaggccg ctggctatcg ccgagggatc
      301 gtcgcccacc acgtcggaga ggaggagggc gagggtgggc gctggggcgg cgagcttcac
      361 caggccgccg cctttgagca ccgagacgtg cttgcggacg gtgttgatct cgcttatgtt
      421 cgccccgctt ctcagcagcg ccccggtgag cttcttcagg tccgcgagct ctatgggcgg
      481 ggcagggtca gccaggagcg ccgaggcgcc gccggagatg agggcgagga gaaggtctcc
      541 ctctaccaga gagcgcgcga gctcctcggc acttcgggcc gcctccacgc tcttttcgtc
      601 cggctcgggg tggccggcga atatgatctc cagcttctcc gaagccgcct cgtggccgta
      661 cctggtgacg ataagcccgc ccgcgatgcg ctcgccgaat agctcctcgg cggcccgggc
      721 catagcgccc gccgccttgc ccgcggccag cacgaagacc ctctccgcct cgaaccgctc
      781 gtccccgacg aggacggtgt tcccctcgac ccgcacgaga cgccgcacgg cctcctcggg
      841 atcagcggcg gcgagactgg cggcgaggat ctctctcaag tgattcttca cgggtgcgtt
      901 ctattcacgg gtgcgttcta atctctttca ggaacccctc cgacgacctg tcccgccatc
      961 tcgcgtgtcc cgtcgaggat gggttcgtac tcgttgatct tgtctataga gagccccatc
     1021 gcggcgttgg tctcccgctc gcaaatgatc tcgacgaggg cagggacccg ccgctcctcg
     1081 ctcgcgcgaa cagcccactc gagggcgcct ttgatctcct ctggcctcgt taccctgcgc
     1141 ccgagagcgc ccatcgcctc cattaccccg acgttgtcta tgccgtactc gttggccggg
     1201 ccctcgtagc cgaggtccac cgcgaagttc atgtcgtagc ccagctccgc ctggcggatg
     1261 aggcccatgt acgcgttgtt gagcatgacg agcacgtagg gaaccctgta ctggacggcg
     1321 accgctacct cctccatgag gaactggaag gagtagtcac ctacgacgcc gactaccagg
     1381 ttctccggac ggccgagctt gacgccgatg cacgcaggca cctcccagcc cagaggaccg
     1441 gcctgcccgc agcagaggta gtggcgaggc ttgtaggtct tctggaactg gccgctgaag
     1501 atctggtata gccctatggc ggtgacgatg atcgcgtcct cgtcgaagaa ctcgttcatc
     1561 tcctggaatg cccgctgcgg cttgaccggc acgtcgtcga agtccatctt ccgcaagagc
     1621 gtcgagcgca gctcgtccac cctatcgacc cactcaccag gttcccgcgc aggcgtcatg
     1681 tcgcgcgcgg tgtcgagcat ctcctgcagg gcgagcctgg cgtccgagac cacagccagg
     1741 tcgggctgga agacccttcc caactggcca gggtctatgt ctatgtggat gaacttgcgg
     1801 tccccgcggt aaacgtcgag atcccctgtg tgccgctcgg cccaccggtt gccgatcccc
     1861 acgaccaggt cgctctccag gaagagggcg ttggcgtagc gctgctgggt ctggagaccc
     1921 acgataccgg cgtagagcgg atggtcctcc gggatggtgc cctttcccat gtaggtaggg
     1981 gtcacaggga tctgcaggta ctccgcgaca tccacgagct cttctgaggc gtcggcgatg
     2041 atgaccccgc cgccaggcat cagcatcggc cgctcggcct ccaggatcat ctccacgact
     2101 tgcctgatgg cgttcgggtt aggcgcgggc tgcgtgaagg gcaaccggcc gccgcgcctc
     2161 gggtcgaagt ccacctccat gccggatttc gtcacgtcga ggggaaggtc tatgaggacg
     2221 gggccgggcc tgccttccct ggccacgcga aaggcctcac ggaaggtcca gacgagctga
     2281 gagggctcct ttacctgcac ggcccacttc gtcacaggct tcgctatgtc cacgatgtcc
     2341 acagcctgga aggcctcctt gtggagcatg ctcgtcgggg cctggccggt tatgcagatc
     2401 tgcggtacgg agtccgccat gcaggtgtag aggccggtga tcatgttggt gccggcaggt
     2461 ccgctggtcc ctatgttgat cccgaccttt cccgtaaccc gcgtataccc gtcggcagcg
     2521 tgtgtccctc cctcctcgtg cctgacagag tagtgcttta tctgatcgga tttgctaagg
     2581 gccttgtaca gcggcaggat cgccgccccc ggtaccccga aggcgacctc gacaccctcg
     2641 tcttccatca ccttcactac cgcgtccatc acgttcatcc tcggcatgga gatcctcctt
     2701 tcccgggtcc cccaacccct attcccctat ggtgtgctgc gagagatcct cgatgattcg
     2761 gagcaaggcc gaatggtcct ccccgcccca ccccttcctc ttcatggaga ggagcatctg
     2821 gtccactatc gcggtgacag gcatcaccac cccatactcc ctgccagcag ccagcgctat
     2881 cccaaggtcc ttgtggtgca gctccgaccg gaaaccgggc tcgaaagtgt gagagaggaa
     2941 cttctccctt ttgacctcca tcaccttgtt tgacgccaag cctccagaga gtacctcaag
     3001 gatcttctcc gcagataccc cacccgcaga gcccaacaca agcgcctctg atacagcctc
     3061 tatggtgagg gctacgacaa cctggtttgc tgccttggtg acctgccccg ctcctgtggg
     3121 tcctacgtgg gttatggtct ttcccatcgc ctcaaggaga ggcttggccc tctggaagtc
     3181 tctctcctga ccgccgacca tgatcgataa agttccttca atggcaccca cgtcccctcc
     3241 gcttaccgga gcgtccagca tgctcgcgcc cttctccttt accgcttcgg ccaggtcctc
     3301 tgtgacgacg ggggagatgg tgctcatgtc tatgagaaga gcaccttcgc tgatgccctc
     3361 cagcacgccg tcctccccgg cgacgacctc ccgcacctgc ggtgagtccg ggagcatggt
     3421 gatgacgacg tcgctgttct ccgccacctc cctcggggtc gcggccacgg ccgccccctc
     3481 cttgcccagc tcctcggcct tctcgggact ccggttgtgg accgtgagct cgtagcccgc
     3541 ttccatcagg ttgcgggcca tgggcatgcc catgatcccc agccctataa agccaaccct
     3601 ctcagccata tcagcctcct cctacaggtt caggtctccg accttcacgt cgctgccgcg
     3661 caggggctcc ggtatccacg cgaagctgtc ctcggtcctc tccgtggtgg ggttgtactc
     3721 gaggccgacg tagccgtcgt aaccgagctc ctcgaggacg ccgagcacgt aagggtagtg
     3781 gatctcaccc gtccccggct ccccgcgcgc cggcgagtca g
//
LOCUS       DASTUV010000105         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1025320, whole genome shotgun sequence.
ACCESSION   DASTUV010000105 DASTUV010000000
VERSION     DASTUV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025320"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1631)
                     /locus_tag="VFT03_02220"
     CDS             complement(216..1631)
                     /locus_tag="VFT03_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU4846988.1"
                     /translation="MQVWTFLAYLVAAALFITGIRRLRRPETARSGNTIAAVGMVIAL
                     VATIFVANIDEALSTVEIGVGVLVGSVIGAVAAQRVQMTAMPQMVAAFNGVGGAAAAL
                     VAVSEFYRVEALGSGQEPLVSAIAVLLSVVIGTISFSGSIIAFVKLQELALGGSVTFP
                     LQQVVNALIALSILVLAANIVTDGAILPFLSPLISLYVIIGAALLLGVLFVIPIGGAD
                     MPIVISLLNAFTGIAAAASGFVLDNYALIISGTLVGASGTILTQNMSGALGRPLGKII
                     FAGIAASGTSKGGDQEDKEVNDTSPEEVGEFLSDEAEKVVIVPGYGLAVAQAQGAIRE
                     LTEALESEGKEVLFGIHPVAGRMPGHMNVLLAEAGIPYEKLYDLEDINPEFEDADAAI
                     IVGANDVVNPAANDPEQDSPISGMPILNVEAADRVIFIKRSLSPGFAGVDNPLFYDTE
                     KTMMLFSDAKQGLQDVTSALK"
     gene            complement(1636..1938)
                     /locus_tag="VFT03_02225"
     CDS             complement(1636..1938)
                     /locus_tag="VFT03_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU4846989.1"
                     /translation="MQELLAQVAIFVIAAFLGFELISRVPNLLHTPLMSATNAIHGIV
                     LVGAIIALAEASDAFTTVVGLTAVVFGAMNVVGGFWVTNRMLGMFRSPVKKVKRRK"
     gene            complement(1942..>2916)
                     /locus_tag="VFT03_02230"
     CDS             complement(1942..>2916)
                     /locus_tag="VFT03_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846990.1"
                     /translation="TVFSNEAIPRTSVAQSMDALSSMGSIAGYKCALIGANALGKYVP
                     MLSTAAGTTKAAKILVFGVAVAGLQAIAITNRLGGEVFAYDIRPETKEQAESLGASFI
                     DSNDEKEDDAGEDEFVEYEPTGFRKLMSSLGFYSFAEPPRDEYVVEGEEEPEEDSEEE
                     EGWSEEKLEEDQKLVRERIKEMDIVITTALVPGRKAPQLVDREMVESMKPGSVIVDLA
                     AEAGGNCELTEPGKTVEHSQVRIIGPLNLPSAMPIHASQLYARNMHNLIGHITEDRAE
                     EKDEHDLHPNLDFEDEIVAATCIAHGGEIRDEATREALDESPEESEAS"
BASE COUNT          469 a          939 c          923 g          585 t
ORIGIN      
        1 gcttcgcccg gcagcttgtc agcacgcttc gggccttttc aaggccctcg ctttcagcat
       61 gccgccttgg gctcttgccc ggtcacgttt tccgggacgg ctttacagcg gtagctctgt
      121 ctagcgctgc ggagcgccgc agtgcgccat cctggctgac aaggcgtctg gcgaagccag
      181 cgccgtgctg acaagcctcc cgaagggagg cgtgcttact tcaaagcgga ggttacgtcc
      241 tggaggcctt gcttggcgtc ggagaagagc atcatggtct tctcggtgtc gtagaagagg
      301 gggttatcca cgccggcgaa gccggggctc agggagcgtt ttatgaagat gaccctgtct
      361 gcggcctcga cgttcagtat gggcattccg gagattgggc tatcctgttc cgggtcgttg
      421 gccgcggggt tgacgacgtc gtttgcgccg acgatgatcg cggcgtccgc gtcctcgaac
      481 tcggggttga tgtcctcgag gtcgtagagc ttctcgtaag ggattccggc ctcggccagg
      541 agcacgttca tgtgtcccgg catgcgccct gcgaccgggt gtatgccgaa gaggacctcc
      601 ttgccctccg attccagggc ttcggtcagc tcgcgtattg cgccctgggc ctgggcgacc
      661 gcgagaccgt agcctggcac tatgacgacc ttttcggctt cgtcggagag gaactcgccg
      721 acctcttcgg ggctagtgtc gttgacctcc ttatcttcct gatccccgcc cttgctcgtc
      781 cccgaagcgg cgatgccggc gaagatgatc ttgcctaaag gccgtccgag ggcgccgctc
      841 atgttctggg tgaggatggt gcccgaggcc ccgacgagcg tgccgctgat gatcagggcg
      901 tagttgtcga ggacgaagcc cgaggcggcg gcggcgatgc ccgtgaaggc gttgaggagt
      961 gagatcacga tgggcatgtc ggccccgccg atgggtatga cgaaaaggac tcccagaagt
     1021 agcgccgcgc cgatgatgac gtagagcgag attagggggg agaggaacgg cagtatcgct
     1081 ccgtccgtca cgatgttcgc cgcgagcacg aggatggaga gcgcgatcag ggcgtttacc
     1141 acctgctgca gcgggaaggt cacagagccg cccagcgcga gctcctggag cttgacgaag
     1201 gctatgatgc tcccggagaa gctgatcgtc ccgataacga cgctcaggag caccgctatc
     1261 gccgagacca gcggctcttg cccactcccg agcgcctcga cccggtagaa ctcggagacg
     1321 gcgaccagcg cggccgcagc gcccccgacc ccgttgaaag cagcgaccat ctgcggcatc
     1381 gccgtcatct gcaccctctg ggccgcgacc gccccgatca ccgatcccac gaggaccccg
     1441 accccgatct cgaccgtact caaagcctcg tctatgtttg ccacgaagat ggtcgccacg
     1501 agcgctatga ccatgcctac ggcggcgatg gtgttccccg aacgcgccgt ctcaggcctc
     1561 ctcaggcggc ggatgcccgt gatgaagagc gcggccgcca ccaggtaggc aaggaacgtc
     1621 cagacctgca ccgggctact tcctcctctt tactttcttg accggggacc tgaacatgcc
     1681 gagcatcctg ttcgtcaccc agaagccgcc taccacgttc atcgcgccga agaccacagc
     1741 cgtcaggccg accaccgtcg tgaaggcgtc gctcgcctcc gcgagcgcga tgatcgcgcc
     1801 gacgaggact atgccgtgta tggcgttcgt cgcgctcatg agcggtgtgt gcagcaaatt
     1861 tgggacgcgg gagatcaact cgaatcccag gaaagctgct atgacgaaga tggcgacctg
     1921 cgcgagcagc tcctgcatgc cctagcttgc ctcgctctct tcggggctct catcgagcgc
     1981 ctcgcgagtt gcttcgtccc ggatctcacc gccgtgcgcg atgcacgtgg cagcgacgat
     2041 ctcgtcctcg aagtccaggt tgggatgcag gtcgtgctcg tccttctcct cggccctgtc
     2101 ctcggtgata tggcctatga ggttgtgcat gttgcgcgcg tagagctggc tggcgtgtat
     2161 gggcatcgcg ctcggcaggt tcagcgggcc gatgatcctg acctggctgt gctcgacggt
     2221 cttccccggc tccgtcagct cgcaattgcc tccggcctcg gccgcgaggt ccacgatgac
     2281 ggacccgggc ttcatgctct cgaccatctc cctgtccacg agctgcgggg cctttctccc
     2341 ggggactagc gccgtggtta tgactatgtc catctccttt atgcgctcgc gcaccagctt
     2401 ctggtcctcc tcgagcttct cctccgacca gccctcctct tcttcggagt cttcctcggg
     2461 ttcctcctcg ccctcgacga cgtactcgtc tctgggcggc tcggcgaagg agtagaagcc
     2521 cagagaggac atcagcttgc ggaagccggt gggctcgtac tcgacgaatt cgtcctctcc
     2581 ggcgtcgtcc tctttctcat cgttggagtc tatgaatgag gcgcccaggg actcggcttg
     2641 ctccttcgtc tcggggcgta tgtcgtaggc gaagacctct cccccgaggc ggttcgtgat
     2701 cgctatggcc tgcaagcccg cgacagccac cccgaagacc aggatcttcg ccgctttcgt
     2761 agtcccggcg gcggtcgaga gcatgggaac gtacttgccc agagcgttgg ccccgataag
     2821 cgcgcatttg tagccggcga tggagcccat ggaggagagc gcgtccatgc tctgcgcgac
     2881 gctcgtgcgc gggatcgcct cgtttgagaa gaccgt
//
LOCUS       DASTUV010000106         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_166839, whole genome shotgun sequence.
ACCESSION   DASTUV010000106 DASTUV010000000
VERSION     DASTUV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166839"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..815
                     /locus_tag="VFT03_02235"
     CDS             246..815
                     /locus_tag="VFT03_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4846991.1"
                     /translation="MYKSEAHRRGLFHRCFHCWIAGQDALGPYLLVQRRAAAKDTWPG
                     YLDVTAAGHLQSGEEPVIGGLRELEEELGLRVDPGRLAPLGTRRIEQEIPQGCDREFH
                     DVFLLLDGTPPRDLCLQEGEVEAVLRVGLDDVEALGAGVSAREYVRGGISDTRVSLAD
                     FVPNEDDYLRRVAGAARRALAGEDPGRIF"
     gene            complement(812..1270)
                     /locus_tag="VFT03_02240"
     CDS             complement(812..1270)
                     /locus_tag="VFT03_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846992.1"
                     /translation="MSNPSVTVSNGARAANLLEEACRQAPGAANAPQLEVVRVLALDI
                     GRQLDALAGIAREDVRPDVLAEAALCCADLANLAACNAPDLPPDAAPRAVEAALLAAG
                     TVEVLGPIIEAGSGDPGTEDLLRDVRSAAWRAGFAARLVEGSSEKGRPAD"
     gene            complement(1279..2121)
                     /locus_tag="VFT03_02245"
     CDS             complement(1279..2121)
                     /locus_tag="VFT03_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEU4846993.1"
                     /translation="MAQNGSSGGKHAKRVARKAHPWIERLARFGYVAYGVVYFLVGVL
                     AVQAAFSGANQAAGQQGALRAVLAAPLGRVLLCIIALGLLAYAAWRLYEGVMDPEHEA
                     RGATGIVKRLDHVLNGLFHGALALTAGHLALGSGGTGGGSPDDWTATLMSQPFGRWLV
                     VAAGVGIVGAGFYQFYKAYKADFRENLKTGEMSLREKRWTTHAGRLGFAARGVVFGVI
                     GIFLIQAALQANPDKARGLGGALATLARQPFGPYLLGGVALGLVAYGVFMFVMARYLR
                     IEPV"
BASE COUNT          369 a          744 c          745 g          356 t
ORIGIN      
        1 tcagctacgg tggtgcgctt cgcctctccg cagcgctaga ctgagctact gccgcaaagc
       61 tttataacaa aacactacca ggcaagatcc gaacggcaga gaaagctaac aagctgacaa
      121 gcgagggccc ctgggcccga gcgtgctgaa gtgctgacaa gctggcgaag caggcgtgct
      181 accctttcct cgtggacgag ttgctcgatg ttctcgacga tctcggaagg cctacaggcg
      241 aggtggtgta caagagcgag gctcaccgcc gcgggctctt tcaccgctgt tttcactgct
      301 ggatcgccgg ccaggacgcc ttgggaccct acttgctagt ccagcgccgg gctgccgcga
      361 aggatacctg gccagggtac ctggacgtca ccgcggcagg gcacctgcaa agcggcgagg
      421 aacctgtgat tggcgggctg cgcgagctcg aggaggagct gggcttgagg gtggatccgg
      481 ggaggctcgc gccgctcggc acccgccgca tcgaacagga gataccgcag ggttgcgacc
      541 gcgagttcca cgacgtcttc ctcctcctcg acggcactcc tccccgggac ctgtgtctgc
      601 aggaggggga ggtcgaggcc gtgctgcgcg tagggctcga tgacgtggag gcgctcggcg
      661 ccggcgtgtc tgcgagagag tatgtaagag gcgggatctc tgacacgcgc gtgagcctcg
      721 cggacttcgt tccgaacgaa gacgattacc tccggcgggt ggccggggct gctcgccggg
      781 ccctcgctgg ggaagaccca ggccggatct tctaatctgc tggccgtcct ttctcggaag
      841 agccttcgac gagccgggcg gcgaaccccg cccgccacgc ggctgagcgc acgtccctga
      901 gcaggtcctc ggtgcctgga tctccgctcc cggcttcgat gatggggccc agaacttcga
      961 ccgtccctgc ggccaggagc gctgcttcga ccgcccgcgg cgcagcgtcg ggtgggaggt
     1021 ccggggcatt gcaggccgcg aggttcgcca ggtccgcgca gcagagcgcc gcttccgcca
     1081 ggacgtccgg tcttacgtct tccctcgcga tgccggccag agcgtcgagc tgccgcccga
     1141 tatccagcgc caggacgcgc acgacctcga gttgaggggc gttcgcggcc cccggcgcct
     1201 ggcggcaggc ttcttcgagg aggttagcag cccgggctcc gttggagacc gtaacggagg
     1261 gattgctcat cgcggcggtc agaccggctc gatccggagg taacgggcca tgacgaacat
     1321 gaagacaccg taggcgacga gcccgagcgc gacgccgccc aggaggtacg ggccgaaggg
     1381 ctggcgggcc agggtcgcta gagcgccgcc aaggcctctg gccttgtccg ggttggcctg
     1441 gagtgcggcc tggatcagga agatccctat cacgccgaag accacgcccc ttgcggcgaa
     1501 accgaggcgt cctgcgtgcg tcgtccacct cttctcgcgg aggctcatct cgcccgtctt
     1561 gagattctcc cggaagtcgg ctttgtacgc cttgtagaac tggtagaagc ccgcaccgac
     1621 tatgcccacg ccggcggcaa cgacgagcca ccgcccgaag ggttgggaca tcagggtcgc
     1681 cgtccagtcg tccggcgagc cgccaccagt gccccccgag ccaagcgcca ggtgcccggc
     1741 ggtaagggcg agagcaccgt gaaacagccc gttgagcacg tggtcgagac gtttcacgat
     1801 ccccgttgcg cccctggcct cgtgctcggg atccataacc ccctcgtaga ggcgccacgc
     1861 cgcatacgcg aggaggccga gggcgattat gcagagcagt accctcccca gcggcgcggc
     1921 gaggaccgcg cgcagcgcgc cctgctggcc ggcggcctga ttggcgccgc tgaacgccgc
     1981 ctgtactgcg agcactccta ccagaaagta gaccaccccg taggcaacgt agccgaaccg
     2041 cgcgaggcgt tcgatccagg ggtgcgcctt cctcgctacc ctcttggcgt gcttcccgcc
     2101 cgacgaaccg ttctgcgcca cgcctagtgc ctcctgaccg tgtggcagct gccccacact
     2161 ctataccccc gccacggggg tttggacaaa gctatgttta ccatggcgtg cgac
//
LOCUS       DASTUV010000107         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_274154, whole genome shotgun sequence.
ACCESSION   DASTUV010000107 DASTUV010000000
VERSION     DASTUV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274154"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..297)
                     /locus_tag="VFT03_02250"
     tRNA            complement(207..297)
                     /locus_tag="VFT03_02250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Ser,seq:gga)
     gene            complement(566..2461)
                     /locus_tag="VFT03_02255"
     CDS             complement(566..2461)
                     /locus_tag="VFT03_02255"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU4846994.1"
                     /translation="MSVVAVRSGRRGTLLAVAGLMLSLFLVALDQTVVGTALPKIIAD
                     LHGFERYAWVTTAYLLASTSMIPVIGKLGDIYGRKWFIVSGVVVFLVGSALCGAAWGM
                     NELIIFRGIQGLGAGMIFSNIFTSVADIFPDPARRAKYQGIFFSVFALSSVVGPTLGG
                     WITDNLDWRWVFYINLPLGLFSLFALPIVLPQSGQRRRAKIDFLGSATITASVVALLL
                     ALSWVGEGEAWDATRVIIGFVVSAVLLAIFIPIELCAAEPVIPISLFRSRVFASASLL
                     MFLVGIGMFGVILYTPLFVQGVLGKSATGSGTVLTPLVFSMTAVGVIGGQIIARVRRV
                     KPFTLFGTVMMTFGVYLLTTLDVSSTQATVALYLAVTGLGLGFIMPTATLAVQSTVER
                     SMLGVATSATQFIRSIGSTVGTAVIGSIVTKGYAEGLRDNAPPQAPQRLVSALENPQA
                     LVSEGAREALTRAASAFPGGEQLVRQVIQVAREALSGSIHDGFVFVLFAVGCAIGAAL
                     LMKNIRLEEVQPVEPEQRGAPASDTAFVPDLAVALRRDAKGSGDEAYATLLDSIGAGI
                     DPAGRRGAAIALSGLADRIESSNGRYPNLLRAAAGLANGRGGGEHERAVYASKNIIRP
                     LADSFRE"
     gene            complement(2508..>2848)
                     /locus_tag="VFT03_02260"
     CDS             complement(2508..>2848)
                     /locus_tag="VFT03_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4846995.1"
                     /translation="TGLSAPKWFVLVILSREDGIGQGEISRLYGVDPSRVTRIGQGLE
                     SDGLVRRERDPEDNRVVRMYLTEEGRLRLRELPELNSQVEGRIREVLSAEEHEELQRM
                     LGLLAEGMKE"
BASE COUNT          555 a          965 c          849 g          479 t
ORIGIN      
        1 gggtcgagac ggagcactgt accgtcggga cgctcgacgt tcatgaagag atccttcgcc
       61 tcttcggtta ccgtgatcat gcccttctcc ttccctcgcc gctgcgggcg atagagcgga
      121 gcgtgcagtg tctttaccga ccctaccaga tatgcgcaag atttaaactc ggagcccgcg
      181 cttcggagag ttcgcgcgag taagagcgga gagggtggga ttcgaaccca cgagacggtt
      241 caacaccgcc tacgcgattt ccagtcgcgc tccttcggcc aactcggaca cctctcctga
      301 tgacgcgggg agagtttacc aaatcctcac cgcccgatgc ccggatcacg gtccccgttg
      361 cgcctccact cagacggaga tacgctctgc agcacggatc ggcgggaggt gcggacggcg
      421 tagttggagc ccgatggcgt gtgcctgatc gaccagacga agcctgcgca caccggagta
      481 tcgttgagct gccgttcgaa aaatcctgcg gcacgtgctg caagacaggc gcaccgccgg
      541 cacaccgtga cctgcagggc caggtctact ctctgaagct atcggccagg ggccgtatta
      601 tgttcttcga ggcgtagacg gcgcgctcgt gctcgcctcc gcctctcccg ttcgccagtc
      661 ctgcggctgc acggagcagg ttcgggtaac ggccattgga ggattcgatg cgatccgcga
      721 gtcccgagag ggcgatcgcc gcgcctctcc gcccggccgg gtcgatccct gcgcctatcg
      781 agtcgaggag ggtagcgtaa gcctcgtctc cggagccctt cgcgtccctc cgcaacgcca
      841 ccgcgaggtc cggcacgaac gcggtatcac tggcaggagc gcctcgctgt tctggctcga
      901 caggctgtac ctcttcgaga cggatgttct tcataagtag agccgcgccg atagcacacc
      961 cgacagcgaa gagcacgaac acgaaaccat cgtggatgga gcctgagagg gcctcgcgcg
     1021 caacctggat gacctgcctc acgagctgct cccccccggg gaacgcagat gccgcacggg
     1081 tgagggcctc cctcgctccc tcgctgacca gagcctgcgg gttctcgagc gcggaaacaa
     1141 gtcgctgagg ggcttggggc ggagcgttat ctcttagccc ctctgcgtag cccttcgtga
     1201 cgatggaccc tatcactgct gtacccaccg tcgagcctat cgagcggatg aactgcgtcg
     1261 ccgaggtagc cacgccgagc atgctgcgct ccaccgtgct ctgcacggct aaagtcgcag
     1321 tcggcattat gaaaccaagt cccaggcctg ttaccgcgag gtacaacgcg acggtagcct
     1381 gggtagagct cacatccagc gtggtcagca ggtagacccc gaacgtcatc atcaccgtcc
     1441 cgaagagcgt gaagggtttg acgcgcctca cacgggcgat gatctgtccc cctatcaccc
     1501 ccacggcggt catggagaag acgagcgggg tgaggacggt ccctgagccc gtcgcgctct
     1561 tgcccagaac cccctgcacg aagagcggtg tgtagaggat gaccccgaac atcccgatcc
     1621 caacgagaaa catgagcagc gaagccgagg cgaagaccct gctcctgaag agcgatatcg
     1681 ggatgacggg ctccgcagcg cacagctcga tggggatgaa gatcgccagc agaacggcgg
     1741 ataccacgaa cccgatgatg acgcgcgtgg cgtcccaggc ttcgccctcg ccgacccatg
     1801 agagggcgag caggagcgcc acgacggagg ccgtgatggt ggctgacccg aggaagtcta
     1861 tcttcgccct gcgtcgctgg ccgctctggg gtagcacgat gggcagggcg aacagcgaga
     1921 aaaggccaag cggcaggttt atgtagaaca cccaccgcca gtcgaggttg tccgtgatcc
     1981 aaccccccaa agtgggacca actacgctcg agagggcgaa tacgctgaag aagatgccct
     2041 ggtacttggc ccgccgcgcg gggtcgggaa agatgtcggc gaccgatgtg aagatgttgg
     2101 agaagatcat gccagcaccc aacccctgta tcccccggaa gattatgagc tcgttcatcc
     2161 cccacgccgc cccgcacagc gcgctcccta ccaggaacac cacgacgcca gagacgatga
     2221 accacttcct gccgtagatg tccccgagct tgcctatgac ggggatcatc gaggtggagg
     2281 ccagcagata ggccgtggtc acccacgcgt acctctcgaa cccgtgcagg tcggcgatga
     2341 tcttgggcag cgcggtcccc acgacagtct ggtcgagcgc caccagaaac agcgagagca
     2401 tgaggcccgc caccgcgagc aacgtccccc gccgcccaga ccgcaccgct acgacgctca
     2461 tccggcggac actccttgca gatgcgcgac cataccttct cccccgatca ctctttcatc
     2521 ccctcggcga gcaggccgag catccgttgc aattcctcgt gctcctccgc gctcaggacc
     2581 tccctgatgc gtccttccac ctggctgttg agctccggta gctcccgcag ccgcagacgg
     2641 ccttcctcgg tcaggtacat cctgaccacc cgattgtcct cggggtcgcg ctcacggcgc
     2701 accagaccgt cactctccaa cccctggccg atccttgtga cccgcgaagg atccaccccg
     2761 tagagacggc tgatctcccc ctgcccgata ccatcctccc tgctcaggat caccagcacg
     2821 aaccatttgg gcgcgctcaa gcccgtct
//
LOCUS       DASTUV010000108         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1283857, whole genome shotgun sequence.
ACCESSION   DASTUV010000108 DASTUV010000000
VERSION     DASTUV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283857"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="glgP"
                     /locus_tag="VFT03_02265"
     CDS             <1..922
                     /gene="glgP"
                     /locus_tag="VFT03_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU4846996.1"
                     /translation="EPITYITNGVHTWSWLAPQMGALLDRHGGGKAWHEEIERPASWS
                     FVDEIPQGELWETHRATKREMAEFVNHRLRGKAGSGPLDPDALTIGFARRFATYKRAT
                     LLLTDPERLRRIAKDPDRPVRFLFAGKAHPADEPAKEFIRALYRASAEDFAGHLVILE
                     DYDMDVARRLVQGVDLWLNNPRRPLEASGTSGQKASLNGAPNFSALDGWWPEAYNGRN
                     GWIIGHEREYASLEEQDTEDAESLYTTLESSIVPLYYDRSASGVPEGWVVMMKEAIKT
                     VAPTFSTQRMVQDYVRELYLPHATRPETRQ"
     gene            1253..2728
                     /locus_tag="VFT03_02270"
     CDS             1253..2728
                     /locus_tag="VFT03_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU4846997.1"
                     /translation="MYTQGSDTDSPHLRRELHLWEAVGISLALMAPSMAANINPQGTA
                     TTIGRAVPLAFALATIGVLLIAWTFVRLTQRFHHAGSVYGFVGATLGARAGVISGWAL
                     MGTYTFYGVVTSTAAGIFGADFLDALGIWTNQPEWAPFLVGAIALAGVWALATANIRE
                     GTRVLLVIEGTTVALILIVSVVILIKLATGTAPNGNTLDFSVFSVPAGTGTSAVFLGV
                     VFGFLSFAGFEAAATLGEEAQEPRRDIPRAILGVAIFGGLYFIFVTAIEVMGFGTDAK
                     GVESFIASGSLLGDLGSQFVAGWVGELITIGAAVSAFGCALACIVGAARLLFALSRDE
                     VGPSPLGAISPRTGVPARSTAAVVIGAYAIMALGWFAFSVAPFDLFVASGTIGTLILL
                     LVYALATIGAAKLSFFSGERRVAAWEIVVPFLALVVIGYTLFRNVYPYPTEAARWYPI
                     IAAAWVVAGVLVTFVRYAATQRAGERLLTEEGLATRSRTEG"
     gene            2796..>3358
                     /locus_tag="VFT03_02275"
     CDS             2796..>3358
                     /locus_tag="VFT03_02275"
                     /inference="COORDINATES: protein motif:HMM:NF028262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846998.1"
                     /translation="MEGEMHAEREMQERTPRPLPVDTLWVRFVFVDHGGIPKTKAVHR
                     DGFEKRVRAGVGLAKGVLALDPSGMLHPESGLSPVGECRLVPDLSTLTPLPFARGQAI
                     VACDMTEPDATTPWEGCPRSALKRVLGRLAERGCRSVASHEAEFYVWGPDGPLDRTPY
                     AASFALTAAAEFVAELAEALEEMGIRP"
BASE COUNT          548 a         1133 c         1107 g          570 t
ORIGIN      
        1 agaacccata acttacatca ccaatggcgt tcacacctgg agctggctag ccccccagat
       61 gggtgccctt ctcgaccgcc acggcggcgg aaaggcctgg catgaggaga tcgaacgacc
      121 cgcgtcctgg tcgttcgtcg acgagatccc acagggcgag ctctgggaga cccaccgtgc
      181 tacaaaacgc gagatggccg agttcgtcaa ccaccgcttg cgcggaaagg ccgggagcgg
      241 gcctctagat cccgacgcgt taactatcgg cttcgcccgc cgcttcgcca cttacaagcg
      301 ggcaaccctc ctcctcaccg acccggagcg gttgcgcagg atcgcgaaag accccgaccg
      361 cccggttcgg ttcctcttcg ccggcaaggc tcaccctgcc gacgagccgg ccaaggagtt
      421 catcagggcg ctgtacaggg catcggcaga agacttcgcc gggcacctcg tgatcctcga
      481 ggactacgat atggacgttg cccgccgcct cgtgcaaggc gttgacctct ggctcaacaa
      541 cccccgccgc cccctggagg ctagcggcac ctccggccag aaggcctccc tcaacggcgc
      601 cccgaacttc agcgccctcg acggctggtg gccggaggcc tacaacggcc gcaacggctg
      661 gatcatcggt catgagaggg agtacgcctc cctcgaagag caggacaccg aagacgccga
      721 gtccctctac acaacactcg aaagctccat cgttcccctc tactacgacc gcagtgcctc
      781 gggcgtcccg gaaggatggg tcgtgatgat gaaggaggcc ataaagaccg tcgcccccac
      841 cttcagcaca cagcgtatgg tccaggacta tgtgcgcgag ctctaccttc cgcacgcaac
      901 gcgaccggaa acccgccagt aggtgacgcg gaagaggctc ccgcgagagc tgttgtcgca
      961 actgcctgtg tatcggtgca ctcgatggct acaggttctg gccgtgatac ctggctggag
     1021 aaagcctccc gacactgtgg aatgttggcc cgtagcgtga gatattggac ccatcatcac
     1081 gggaaagtga gatgccagag tgtgtgatgc aagccgagcg cgaggcacaa gagcacgtgc
     1141 cgcggccgtt gcctgccagc cgccttctgg ccgtggaagc cggtgaggtg cgtatggacc
     1201 cgggctgacc ccgccctcga ttctccgtaa cccacagagc aagggagtca ccatgtacac
     1261 acaaggcagc gacaccgaca gcccgcacct gcggcgtgag ttgcacctgt gggaggcggt
     1321 cggcatctcg ctggccctga tggcgccttc gatggccgcc aacatcaacc cgcagggcac
     1381 cgctaccacc atcggacgcg ccgttcccct ggcgttcgcg ctggcgacca tcggcgtctt
     1441 gctgatcgcc tggaccttcg tccggctcac ccagcgcttc caccacgcag gcagcgtgta
     1501 cggcttcgtc ggcgcgacgc tcggcgcgcg ggctggcgtt atctccgggt gggcgctgat
     1561 gggcacgtac actttctacg gcgtcgtcac gtcgacagcc gccggcatct tcggcgcgga
     1621 cttcctcgac gccctcggca tctggacgaa ccagccggag tgggcgcctt tcctggtcgg
     1681 cgcgatcgct ctcgcgggcg tgtgggcgct cgcgaccgct aacatccgcg agggcacaag
     1741 agtgctcctt gtcatcgagg gaacgacggt cgcgctcatc ctcatagtca gcgtcgtcat
     1801 cctgatcaag ctcgccaccg gcacggcccc gaacggcaac accctggact tctcggtgtt
     1861 ctcggtgccg gccggcacgg gcacctcggc cgtgttcctc ggcgttgtgt tcgggttcct
     1921 ctccttcgcg ggcttcgagg cggccgcgac gctgggcgag gaggcgcagg agcctcgccg
     1981 cgacataccg cgcgcgatcc tcggcgtcgc gatattcggc ggcctgtact tcatcttcgt
     2041 aaccgccata gaggtgatgg gcttcggcac cgacgccaag ggagtggaga gcttcatcgc
     2101 ctcgggctcg ctgctcggcg acctcggcag ccagttcgtc gcggggtggg tcggcgagct
     2161 catcaccatc ggcgccgcgg tcagcgcctt cggctgcgcg ctggcttgca tcgtaggggc
     2221 cgcgcggctg ctgttcgcgc tctcgcgcga cgaggtcggc ccttccccgc tcggagccat
     2281 ctccccgagg accggcgtcc cggcgcgctc cacggccgcc gtcgttatcg gcgcgtacgc
     2341 catcatggcc ctcggctggt tcgcgttcag cgtggcgcct ttcgacctct tcgtggcgtc
     2401 gggcacgata gggaccctga tcctgctgct cgtttacgcg ctcgctacga tcggagccgc
     2461 gaagctgtcc ttcttctccg gcgaacggcg ggtcgcagcg tgggagattg ttgtgccgtt
     2521 cctcgcgctg gtagtcatcg gctacacgct cttccgcaac gtgtacccct acccgaccga
     2581 ggcggcgagg tggtacccga tcatcgccgc cgcctgggtc gtcgccggag tcctggtcac
     2641 cttcgtccgc tacgcggcga cacagcgcgc cggcgagagg cttctgaccg aggagggcct
     2701 cgctacgcga tccaggacgg agggctagaa catgtagctc cggcgtgaga tattggacac
     2761 acccctgtgg gaagggaaag gtcttcggct aggagttgga gggagagatg cacgccgagc
     2821 gcgagatgca ggagaggacg ccgcggccgt tgccggtaga tactttgtgg gtgcggttcg
     2881 tgttcgtgga tcacggcggc atcccgaaga ccaaagccgt ccacagggac ggcttcgaga
     2941 agagggtgag ggcgggcgta gggctcgcca aaggagtgct ggcgctcgac ccgagcggga
     3001 tgctgcaccc cgagtcgggg ttgagccccg taggggagtg ccgcctcgtc ccggacctct
     3061 cgacgctcac cccgctcccg ttcgcccgcg gccaggcgat cgtcgcctgc gacatgacgg
     3121 agccggacgc gaccacgccc tgggaaggat gcccgcgcag cgctctaaag agggtcctgg
     3181 ggcggcttgc cgagaggggg tgcaggtccg tcgcctccca cgaggccgag ttctacgtgt
     3241 ggggtcctga cggcccgctg gacaggacgc cttacgccgc gagcttcgcc ctgacggcgg
     3301 ccgcggagtt cgtcgcggag ctggctgagg cgttggagga gatgggcatc aggccgga
//
LOCUS       DASTUV010000109         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_881786, whole genome shotgun sequence.
ACCESSION   DASTUV010000109 DASTUV010000000
VERSION     DASTUV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881786"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFT03_02280"
     CDS             <1..429
                     /locus_tag="VFT03_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4846999.1"
                     /translation="RTAATGALVLWHELAHAFLLSEPLPRTPAWLRELVPQALSAAVA
                     RKTGLPLERHLEKIDRSPGFTIRSFGGRADAEAQMAFQNLLLALGDAAVEEFGNGFLR
                     ALAHALWEETDVVGEERAEALLADTLRPGGREWLASRPEI"
     gene            437..1504
                     /gene="leuB"
                     /locus_tag="VFT03_02285"
     CDS             437..1504
                     /gene="leuB"
                     /locus_tag="VFT03_02285"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU4847000.1"
                     /translation="MRDSFDILLLPGDGIGPEVVSAAIQVMDAAADHFGVELSYEERK
                     VGAAAIRDEGEPISEETLERAKSSDSVLLGAVGHPDFDRGTVRPEAGLLRLRKSLGAF
                     ANLRPVAAIPSLLSASTLKEDVLKGVDILIVRELTGGAYFGEKEEGEEKASDLSVYTR
                     EEVERVARVAFEAAEKRKGRVTSVDKANVLATSRLWRGVVEDVAGEYPNVALDHVLVD
                     AAAMFLIRDPRRFDVILTENLFGDILSDEAAMLPGSMGMLPSASLGEPGAPGIYEPVH
                     GSAPDIAGSNKANPYAAILSAAMMFRHSLGRPDVAGAIEQGVSVALEAHYLSPDLGGN
                     KTTEEVTDAVSRWVAAGEGVI"
     gene            1501..3051
                     /gene="cimA"
                     /locus_tag="VFT03_02290"
     CDS             1501..3051
                     /gene="cimA"
                     /locus_tag="VFT03_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEU4847001.1"
                     /translation="MSIRLFDTTLRDGTQREGVSLTAEDKARIAHELDSLGVHYVEAG
                     FPASNPKDLEFFEGFDASALENARLVAFTRARRPNGRAEDDPAVTLLASLSAPVACIV
                     AKSWRLHVEKVLRTTPEENLESVRDTVAYLAGAGKEVIFDAEHYFDGFRDDPEYSLAV
                     LRAAAEAGATTLVLCDTNGGTLPTELKAIVARTVREFPEVEVGIHSHNDGGCGVANAL
                     VAVEAGATHVQGTVNGVGERTGNCDLVTTIANLQLKMGLKVIDDERLEKLTETSNYVA
                     ELMNLTPDTHRPYVGRSAVAHKGGLHVDGIRQDPRTYEHVAPERVGNVRRTPVGELSG
                     KNSIRAKAEELGLEPGDVTSVLTAIKQREYEGYHYEAADASLALLIGRTTGEAKPLFE
                     LESLRIISEKRADGETTVEATIKLYVKGQREISTAEGNGPVNALDKALRQAIEPHYPE
                     LKEIHLSNYKVRILDEHRATAATTRVLIDSTDGKRVWGAVGVGENIIEASWQALVDGL
                     EYGVNRTE"
     gene            complement(3184..>3383)
                     /locus_tag="VFT03_02295"
     CDS             complement(3184..>3383)
                     /locus_tag="VFT03_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847002.1"
                     /translation="EYRQHRGPHPSGWHSFPIGHFPIGHFPIGHFPIGHFPVEPSPVE
                     PSPVESSPDQRAWRRALWGHQ"
BASE COUNT          615 a         1072 c         1185 g          511 t
ORIGIN      
        1 cgcaccgccg ctacgggcgc gctggtgctc tggcacgagc tggcgcacgc cttcctgctg
       61 agcgagcctc tcccccgcac cccggcctgg ctccgggagc tcgtgccgca agccctctcc
      121 gcggcggtcg cccgcaagac cggtcttccg ctcgagaggc atctggaaaa gatagaccgc
      181 agtcccggct tcaccattcg tagcttcggg ggacgggcgg acgcggaagc gcagatggcg
      241 ttccagaact tgctgctcgc gctcggggac gcggcggtgg aggagtttgg caacggattt
      301 ttgagagccc tggcgcacgc gctgtgggaa gagaccgacg tcgtcggcga agagcgggcc
      361 gaggcgttgc tggccgacac tctacgccct ggcgggcggg agtggctcgc gtcgaggccg
      421 gagatctagg aggaagatgc gcgactcttt cgacatattg cttctccccg gcgacggcat
      481 cggccccgag gtcgtcagcg cggccataca ggtcatggac gctgccgcgg atcacttcgg
      541 ggtcgagctc tcctacgagg agcgcaaggt cggagccgcc gcgatccggg acgaaggcga
      601 acccatctcg gaggagacgt tggagcgggc gaagtcctca gactccgtcc tgctcggagc
      661 ggtaggacac cctgacttcg acaggggaac ggtgcgtccc gaggccggtc tgctcaggct
      721 gcgcaagtct ctaggggcgt tcgccaacct gcgccccgtc gccgcgatac cgtcgttgct
      781 ctccgcttcg acgctaaaag aggacgtatt gaagggagta gacatcctca tcgtccgcga
      841 gctgaccggc ggggcctact tcggggagaa ggaggagggc gaggagaagg cgagcgatct
      901 ctccgtctac acccgcgagg aggtcgagag ggtggcccgc gtcgcgttcg aggccgcgga
      961 gaagcgcaag ggaagggtaa cgtcggttga caaggcaaac gtcctggcga cgagccggct
     1021 gtggaggggc gtggtcgaag acgtggcggg cgagtacccc aacgtcgccc tggatcacgt
     1081 cctggtagac gcagccgcga tgttcctgat ccgggacccc cggaggttcg acgtgatcct
     1141 gaccgagaac ctcttcggcg acatcctctc ggacgaggcg gccatgctcc ccggctctat
     1201 ggggatgctg ccttccgcgt ctcttggcga gcccggcgct cccggcatct acgagccggt
     1261 tcacggctcg gcccctgaca tcgcgggcag caacaaggcg aacccctacg ccgcgatcct
     1321 ctctgcggcg atgatgttcc gccactctct ggggcgcccg gacgtggccg gagcgatcga
     1381 gcagggcgtc tcggtcgcgt tggaggctca ttacctgagc ccggatctgg ggggaaacaa
     1441 gaccacggaa gaggttacgg atgccgtgag caggtgggtg gcggcggggg aaggcgtgat
     1501 atgagcataa gattattcga cacgaccctt cgggacggga cccagcgcga gggcgtgagc
     1561 ctgaccgcgg aggacaaggc tcgcatagcg cacgagctcg actcactggg ggtccactac
     1621 gtggaggctg gcttcccggc ctcgaacccc aaggacctgg agttcttcga gggcttcgac
     1681 gcctcggctc tcgagaacgc caggctcgta gccttcaccc gcgcccgccg cccgaacggg
     1741 agggcagaag acgatcccgc ggttacgctg ctcgccagcc tctccgcccc cgtcgcgtgt
     1801 atcgtcgcca agagctggag gctgcacgtc gagaaggtct tgcggaccac gccggaggag
     1861 aacctggagt cggtgaggga cactgtcgcc tacctcgccg gggctggcaa agaggtcatc
     1921 ttcgacgccg agcactactt cgacggcttc cgggacgacc ctgagtactc tctggcagtg
     1981 ctgcgcgccg ccgcagaggc cggggcgaca accctcgtcc tctgcgacac caacgggggg
     2041 acgctcccga ccgagctcaa ggccatcgtc gcgcgcaccg tgagggagtt ccccgaggtg
     2101 gaggtcggga tccactccca taacgacggc gggtgcgggg tggctaacgc gctcgtcgcg
     2161 gtcgaggcgg gggcgacgca cgtccagggt accgtgaacg gcgtgggcga gcgtaccgga
     2221 aactgcgacc tggtaaccac catcgccaac ctgcagctca agatgggcct caaggtaata
     2281 gacgacgagc gtctcgagaa gctcaccgag acctcgaact acgtcgccga gctcatgaac
     2341 ctcacccccg acacgcaccg gccctacgtc ggccgcagcg ccgtcgccca taagggcggg
     2401 ctgcacgtcg acggcatcag acaggatccg aggacctacg agcacgtcgc gcccgagcgc
     2461 gttggcaacg tcagacgcac gccggtcggg gagctctcgg ggaagaactc gatccgggct
     2521 aaagccgaag agctcggcct ggagcctgga gacgtcacgt ccgtcctcac cgccataaag
     2581 cagcgggagt acgagggtta ccattacgaa gcggccgacg cttcgctcgc gctgctcata
     2641 ggccggacga caggcgaggc gaagcccctc ttcgagctcg agagcctccg aattatctcc
     2701 gagaagcggg ccgacggcga gacgacggtc gaggcgacca taaagctcta cgtcaagggg
     2761 cagcgcgaga tctccaccgc cgagggaaac ggtcccgtca acgccctcga caaggccctg
     2821 cgacaggcca tagagccgca ttacccggag ctgaaggaga tacacctctc caactacaag
     2881 gtccgcatcc tcgacgagca cagggcgacc gccgcgacga cgcgggtgct catagactcg
     2941 accgatggca agcgtgtctg gggcgccgtg ggggtaggcg agaacatcat cgaagcttcc
     3001 tggcaggcgc tcgtggacgg gctcgagtac ggtgtcaaca ggaccgaata gcatttcagc
     3061 acgcctgcga agccggagcg tgctgagaat cagtcgtcga acggaccgcc gctgagctgg
     3121 acggggtcgt tgccgccgac gttcacattc acgtgggtct cgccatcgct ctgaggcggc
     3181 gggctactgg tgcccccaga gggcgcgcct ccaggctctc tggtcggggg aggactcgac
     3241 gggggagggc tcgacggggg agggctcgac ggggaagtgc ccgatgggga agtgcccgat
     3301 ggggaagtgc ccgatgggga agtgcccgat ggggaagctg tgccatcctg acgggtgggg
     3361 tcctcgatgc tgtcggtatt ccc
//
LOCUS       DASTUV010000110         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1062520, whole genome shotgun sequence.
ACCESSION   DASTUV010000110 DASTUV010000000
VERSION     DASTUV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062520"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFT03_02300"
     CDS             <1..922
                     /locus_tag="VFT03_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEU4847003.1"
                     /translation="YEKPQDATFRLADDGGVEVQEAREGHVLNQEGTLANLDRTLTDL
                     SNQVPIAEGSSPKPSITTEEVEKLKPTEVIGDYKTDFRWDSNPSRKANMKLAAGAING
                     TLVAPGEVFSFNEHASELDYNKAKTFSDGGVGYADGGGLCQVSSTLYMAAQYAGLEIV
                     ERHPHYAVLPYIKPGFDATVWFGEPGTGWGAMDMRFRNTTDAYILVREWVDSNGFLNA
                     EIQGQPTGKKVEMRTEKIYESTARGIKWATYKKVTKDGKVLFDGLLHTYIYSYNPPVD
                     ENLPHYDTSVPRVAGWKDPGNTTGWAEVPQ"
     gene            997..>2116
                     /locus_tag="VFT03_02305"
     CDS             997..>2116
                     /locus_tag="VFT03_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4847004.1"
                     /translation="MPRKKIFDARRRRRRQRFGFLAVLLLVLVAGAVYLAGHASGPDA
                     ETAQQPSGEKPSPVQRVLRAATGESPGEPVPASATETPEGAAYWTVAPEMPGVGPDSV
                     TGVYRSKLDPSWASVRFAPEGESKPFFVFVERAGDEWEAKKSMRADEADYAKNEVVPL
                     AEVPKDLIQYIYPENAFVADVPEPKQEKVDELPSAGPANFTPPEPVSDGVPDAQRERV
                     EKALGAAAQRIEGYDGVAGVYVHDLEGDFSYGVRPDEEFFTASTVKLPIMVAVYRKVD
                     EGELSFSQMIEIKEEDWAAGAGWLQWERAGTRQSVGDLLLLMMTQSDNVAANALVRTV
                     GGADHVNEVARSLGARNTLVYQKVSSERAAIPQLDNRST"
BASE COUNT          406 a          682 c          729 g          299 t
ORIGIN      
        1 ctacgagaag ccccaggatg cgaccttccg cctggcggac gacggcgggg tcgaggtcca
       61 ggaggcccgt gaaggccacg tcctgaacca ggagggcacc ctggctaacc tcgaccgcac
      121 gctgacggat ctcagcaacc aggtcccgat cgccgaaggc tcctctccga aaccctcgat
      181 tacaaccgag gaggtcgaga agctcaagcc gacggaggtc ataggggatt acaagacgga
      241 tttccgctgg gactcgaacc cgagccgcaa agccaacatg aagctcgccg ccggagccat
      301 aaacggcacc ctcgtggcgc ctggcgaggt gttctcgttc aacgagcacg cttcggagct
      361 tgactacaac aaggcgaaga ccttctcgga cggtggcgtg ggctacgccg acgggggcgg
      421 cctctgccag gtctcttcga cgctgtacat ggcggcccag tacgcggggc tcgaaatcgt
      481 cgagcgccac ccgcactacg ccgtgctgcc gtacataaag cccgggttcg acgcgacggt
      541 gtggttcggt gagccgggga ccgggtgggg cgccatggac atgaggttca ggaatactac
      601 cgacgcgtac atcctcgtcc gcgagtgggt ggactcaaac ggcttcctca acgccgagat
      661 ccagggccag ccaaccggca agaaggtcga gatgcgcacc gagaagatct acgagagcac
      721 cgcgaggggc atcaagtggg cgacctacaa gaaggttacg aaggacggga aggtgctctt
      781 cgacggcctc ctacacacct acatctacag ttataacccg cccgtggacg agaacctgcc
      841 gcactacgac acgagcgtgc cgcgggtcgc cggctggaag gaccccggca ataccaccgg
      901 ctgggccgag gtgccccagt gaggcaaaga acggaccgtg ccgccacgcc tcctcggggg
      961 cgctgacccg gcgcctcagg agccccattg aatttcttgc cacgcaaaaa gatcttcgac
     1021 gcccgccgcc ggcgccgccg gcagcggttc ggatttctgg cggtgttgct cctcgtgctc
     1081 gtggcgggcg ccgtgtacct cgcgggccac gcctctggcc cggacgcgga gacggcgcag
     1141 cagccgtcag gggagaagcc ttcgccggtc cagagggtat tgcgggcggc aacgggggag
     1201 agcccgggag agcccgtccc ggcgtccgcc accgagacgc ctgagggggc agcctactgg
     1261 accgtggccc ccgagatgcc cggcgttggc cctgacagcg tcacgggcgt ttaccgcagc
     1321 aagctcgacc cgtcgtgggc ctcggtgcgc ttcgcgccgg agggggagag caagcccttc
     1381 ttcgtcttcg tcgagcgggc aggcgacgag tgggaggcta agaagtccat gcgggccgac
     1441 gaggccgatt acgccaagaa cgaggtagtc ccgctggccg aggtgcccaa ggacctcatc
     1501 cagtacatct accccgagaa cgcgttcgtc gcggacgtcc ccgagcccaa acaggagaag
     1561 gtggacgagc tgccgagcgc cgggccggcc aacttcacgc cgcccgagcc ggtttcagat
     1621 ggtgtccccg acgctcagcg cgagcgcgtg gagaaagcgc tcggggcggc ggcgcagagg
     1681 atcgagggct acgacggcgt ggcgggcgtc tacgtacacg acctggaggg tgacttcagc
     1741 tacggggtgc gccccgacga ggagttcttt accgcctcta ccgtcaagtt gccgatcatg
     1801 gtggccgtct accgcaaggt ggacgagggc gagctgagct tctcgcagat gatcgagatc
     1861 aaggaggagg actgggcggc aggggccggg tggctgcagt gggagagggc ggggaccagg
     1921 caatccgtgg gcgacctcct tctcctgatg atgacccagt cggacaacgt cgccgccaac
     1981 gccctcgtgc gcaccgtcgg gggcgctgac cacgtcaacg aggtcgcccg ctctctgggc
     2041 gcccggaaca ccctcgtcta ccagaaggtg agcagcgagc gggcggccat cccgcaactg
     2101 gacaacagga gcacgc
//
LOCUS       DASTUV010000111         5490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_201987, whole genome shotgun sequence.
ACCESSION   DASTUV010000111 DASTUV010000000
VERSION     DASTUV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201987"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1277)
                     /locus_tag="VFT03_02310"
     CDS             complement(264..1277)
                     /locus_tag="VFT03_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4847005.1"
                     /translation="MEEVDQVPEITDPPPDEVSADLATLRSALDDDKNGGVPARALDR
                     NLLIATWNIRAFGDLTEKWESADGDSPKRDLHSLLCIAEIISRFDVIAIQEARDNLKA
                     LRHLIHVLGPNWGLSLTDVTEGAPGNGERMAFLFDTRRVQLSGLACEIVVPQEQLERI
                     EPGALQMQFARTPYAVSFRSGEKTFILITLHILYGESEEERLPELKAIAEWLAGWARD
                     INSWDHNLIALGDFNIDRRGDALHDAFVSTGLEIPQDLQEAPRTIFSDPQKPELNKFY
                     DQIAWFTGRNDLPALSLQYSRGGYFDFTRAALTTRELTKVQLSWRISDHYPLWAEFSA
                     RSG"
     gene            1592..2788
                     /locus_tag="VFT03_02315"
     CDS             1592..2788
                     /locus_tag="VFT03_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEU4847006.1"
                     /translation="MRRFTLALSAVFLSTAVALVASHFALSETSKADRTDSGGSVSEV
                     YAHAGQAQYATPTRVEPEPPETDYYSFYDHWLAQQGDSPEVPQTEDAVSEGSTPEVRA
                     PAEALSAMEPSEAYSQVVDNDSARRFSARGWDERSGIAPSHGKDFSYAWPQRDPASAR
                     FRVRIPAKDYYTVYARWPAARGNNPGTRFGIRTSSGIRWTEVDQRRDGAMWVRLGDYE
                     MEAGYRTAVRVSGDAKKDGRVVADAIKVVRGTQTAPPRGDAAKRESDGGATVGSGRVN
                     GLEVVRRARAHIGTPYRHSPPLPCEAFRSEDCSCLTSRVFSKWLTLPDDPVAQWRMGE
                     RVAKSDLLPGDLVFFKEAGKANPITHVAIYSGSNNIIHASTYWGHVVERPLDQVSGYY
                     GAKRLS"
     gene            complement(2792..3019)
                     /locus_tag="VFT03_02320"
     CDS             complement(2792..3019)
                     /locus_tag="VFT03_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847007.1"
                     /translation="MNLNLTPPAEGFDLYRHKDAGETRAGGWSWLYWARRNEAGDYEI
                     RAITGAGEAYSVPGGVFPRKGFEEHYEKVEP"
     gene            complement(3016..3654)
                     /gene="lexA"
                     /locus_tag="VFT03_02325"
     CDS             complement(3016..3654)
                     /gene="lexA"
                     /locus_tag="VFT03_02325"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU4847008.1"
                     /translation="MTEDLHPSRLRILEFLARRDPTEGPPAVREVAEAVGLRSTQTVH
                     HHLLKLEEDGYIDRGSIPGRKRRPLRLTDKGWDAVSTRPLLGRIAAGRGLEAMANEEP
                     YSLAAELLAARPGSRRFLLEARGDSMTGAGIEEGDTLVVEENPSPPDGSVVAALILDR
                     EEEATVKVLRRQGETIRLEARNGAHEDIVVPADRVVVQGTVEWVIRRVRGRR"
     gene            3927..4358
                     /locus_tag="VFT03_02330"
     CDS             3927..4358
                     /locus_tag="VFT03_02330"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEU4847009.1"
                     /translation="MLATVSEIHRYPLRPPGFEEPLFATAGSRRAVAEHLARATWLSR
                     GSDLRRGSGQTAHSREEPAHVLRRADGRAYLVRRCGKGRRTRKRRVVRVLEHWREVGL
                     WWEPERRVDRLVFRVLLSGGAVVDLALERASGEWLLVGMVD"
     gene            4360..>5490
                     /locus_tag="VFT03_02335"
     CDS             4360..>5490
                     /locus_tag="VFT03_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU4847010.1"
                     /translation="MRSQDPGQGFAHLHVRSGFSYGFGVATPAELATAASEMGIEALA
                     LTDRDGLSGIPRFFEAAAGAGVSPVLGAEVSVDGGGHLVLLADSMTGYRSLCRLITRY
                     RCGSEDRRKPLCPFPLLLEHAEGLVCLTGAVPFGHVPRLVLSGLTGTADRVLRALREA
                     FGEGLYVELTDDRTAGSRRRMGRVTELARRNCLPVLATNEVAYLAPEDHKLHDVVVAT
                     SNLTALPGPGYRTTDQLYLKSPEQVEKLFRDRPEAGGNAAEVAERCAGAVDLGGEIHV
                     PSARLRGGETARGKLARLALRGAKARYGHVEGRVRARLLRELGCIEELGFAPYFLLAR
                     EAAEIAREKGVPVTGRGSAANSLVSYCLGLTRPEPFSNRLLFE"
BASE COUNT          881 a         1776 c         1854 g          979 t
ORIGIN      
        1 tgtcagcacg ctcaggcttc gccttcgctt gtcagctaga aagcaaggag ggtgcgacat
       61 gatcttccct gcgtcagtcc aaggatatgc ccaaaagcgt ctctccaagc gtgaccgggt
      121 gtgagcctaa cggcagagag agagctgaaa tgctgacaag cgagggccat tttaaaggcc
      181 cgagcgtgct gagaaggcgt ctggcgaagc cagcgccgtt ctgctaagcc gaccgaaggg
      241 aggcgtgctg acaagcgacg gcgtcagccg gagcgagctg aaaactcggc ccagagcggg
      301 tagtggtcgg agatgcgcca cgagagctgc actttcgtca attcccgtgt tgtcaaagcc
      361 gccctggtga agtcgaagta gcctcctctc gagtattgca gcgagagggc ggggaggtcg
      421 ttcctgcccg tgaaccacgc gatctggtcg tagaatttgt tgagctcggg cttctgcggg
      481 tcgctgaaga tcgtgcgcgg cgcctcctgg aggtcctgtg ggatctccag gcccgtagaa
      541 acgaaggcgt cgtggagcgc gtcgcccctg cggtcgatgt tgaagtcgcc cagggctatg
      601 aggttgtggt cccagctgtt tatgtccctg gcccaccctg cgagccattc ggctatggcc
      661 tttagctcgg ggaggcgctc ctcttcactc tccccgtaga ggatgtgcag cgtgatgagg
      721 atgaaggtct tctctcccga gcggaagctc accgcatagg gtgtgcgggc gaactgcatc
      781 tggagggcgc cgggctcgat gcgctcgagt tgctcctggg gcacgacgat ctcgcaggcg
      841 agtcccgaga gctggaccct tctcgtgtcg aacaggaacg ccatgcgctc gccgttgccg
      901 ggagcgccct ccgtgacgtc ggtaaggctc aggccccagt tgggtccgag cacgtggatg
      961 aggtgcctga gcgccttgag gttgtccctg gcctcctgga tcgctatgac gtcgaacctg
     1021 gagatgatct cggctatgca cagcagcgag tgcaggtcgc gcttgggcga atctccatcc
     1081 gcggactccc atttctcggt gaggtctccg aaggcgcgta tgttccaggt ggcgatcagc
     1141 aggttgcggt cgagtgccct ggccggcacg cctccgttct tatcgtcgtc gagcgccgag
     1201 cgtagcgtgg caaggtcggc cgatacctca tcgggaggcg ggtcggttat ctcgggcacc
     1261 tggtccacct cctccacgcg cgtctacctc ccctggctgc cgagtcgggc gcacccccgg
     1321 cagcgccgct tgccgcccgt gtgcggtact cgttatccat gttgttgcag gtgttgcact
     1381 cggtgttgtc atggtacacc tcgctccagg tcgagtgcca ggggctcctt tcggtcatcg
     1441 tctctcctct cttgtggtat ggcctgatct tcgctcggct ctctatagaa gcgaaaggcg
     1501 atgaattcgc gatgaatcga cgtatcgcgc cgcatgtacc gttcgtatat aatgctgccg
     1561 cacggcactg agaatcttaa gggagggctc tgtgcgtcgg tttacgctgg ctttgtcggc
     1621 ggtgtttctg tctacggctg tagcgctggt tgcctcgcac ttcgccctgt cggagacctc
     1681 gaaagcggat aggacggact ccggaggatc ggtctccgag gtctacgccc acgccgggca
     1741 ggcgcaatac gctacgccca cgcgcgtgga gccggagcct cccgagacgg attactattc
     1801 cttctacgac cactggctcg cgcagcaggg cgattctccg gaggttccgc agacggagga
     1861 cgcggtctcc gagggttcga cgcccgaggt gcgggcgccc gcggaggcac tttcggccat
     1921 ggagccgtca gaggcgtact cacaggtcgt ggacaacgac tcagcgcggc gtttctcggc
     1981 gcggggctgg gatgagagat ctggaatcgc cccatcccac ggcaaggact ttagctacgc
     2041 ctggccccag agagacccgg cttccgcgcg gttcagggtg agaataccag ccaaagacta
     2101 ctacaccgtc tatgctcgtt ggcccgccgc caggggtaac aacccaggca ctcgtttcgg
     2161 tatccgcacc tcctccggga tcaggtggac cgaggtcgac cagcggcggg acggcgccat
     2221 gtgggtgagg ctcggcgact acgagatgga ggctggctat cggaccgccg tacgggtctc
     2281 cggggacgcg aagaaagacg gccgggtggt cgccgacgct atcaaggtcg tcaggggtac
     2341 tcagaccgcg cccccacgcg gggacgccgc aaagagggag tcggacggcg gagcgaccgt
     2401 cggaagtggc cgggtcaatg gcctggaggt ggtccgccgg gcgagagccc acataggcac
     2461 cccttacagg cattcgccac cgttgccttg cgaggctttc cgctcggagg actgctcttg
     2521 ccttaccagt agggtcttca gcaagtggct gacgctaccg gacgacccgg tcgcacagtg
     2581 gaggatgggg gagagggtcg cgaagtcgga cctccttccc ggagacctgg tgttcttcaa
     2641 ggaggccggg aaagccaacc ccataaccca cgtcgctata tactcgggca gcaacaacat
     2701 catccacgcc tccacctact ggggccacgt cgtcgagagg ccgttggatc aagtgagcgg
     2761 ctactacggg gcgaagaggt tgagttgagt ctcagggctc gaccttctcg tagtgctctt
     2821 cgaacccttt tctagggaag acgccgcccg gcaccgagta cgcctcgccc gccccggtta
     2881 tggcccggat ctcgtaatct ccggcctcgt tgcgcctggc ccagtacagc cacgaccagc
     2941 ctcccgctct cgtctcgcct gcgtccttgt ggcggtagag gtcgaagccc tccgccggcg
     3001 gcgtcaggtt caagttcacc ttcggccccg cacgcgccgg atcacccact cgaccgtccc
     3061 ctgaaccacg accctatctg ccggaaccac tatgtcctcg tgggcgccgt tacgcgcctc
     3121 gagcctgatc gtctcaccct gccggcgcag caccttgacc gtcgcttcct cctctctgtc
     3181 caggatgagc gccgcgacca ccgacccgtc agggggcgag ggattctcct ctaccaccag
     3241 ggtatctccc tcctctatgc cggcgcccgt catggagtca cccctggcct ccagcaagaa
     3301 tcgcctgctg cccggccggg cagccaggag ctcggccgcc agagagtagg gctcctcgtt
     3361 cgccatcgcc tccaggccac gccccgcggc gatcctgccc aacagcggcc gggtggagac
     3421 cgcgtcccag cccttgtcag tgagcctcaa agggcgccgt ttgcggcccg ggatactgcc
     3481 ccgatcgatg tacccatcct cctcgagctt gaggaggtgg tgatggacgg tctgcgtgct
     3541 cctcagtcct acagcctccg caacctcgcg cacagcgggc ggcccctccg tcgggtcgcg
     3601 gcgcgctagg aactccagta tccgtagccg gctgggatgc aggtcttcgg tcaaaggcgc
     3661 ctcctgggcc ctactctagc gcaagaaccg cttgcagtca aacattcgtt tggtgtagag
     3721 tgagaagggg aaaccgaaga agaggagcgc ccctgccccg ggccctccgg cggaccgtac
     3781 cacagtggcc agaccgaggc ggtccttcgg ggggtttgtg ggggcagaga acgctcgccc
     3841 ttaccgctgg attgtaccgg atggggctgg ctttctcacc ccatcgggcg ccgccgagca
     3901 tcgctcctct ctggagagga gggaatttgc tcgcgacggt ttcggagatc caccgctacc
     3961 cattgcgccc gcctggattc gaggagccac tctttgccac agccgggtcc aggagggccg
     4021 tcgcggagca cctggcgcga gccacctggc tctcccgggg gtcggatctg cgccggggga
     4081 gcggccagac agctcacagc cgcgaggagc cagcgcacgt gctccgccgt gccgacggcc
     4141 gggcgtacct agtacgccgc tgcgggaagg ggcgccgcac gcgcaagagg cgggtggttc
     4201 gggtcctgga gcactggcgg gaggtagggc tctggtggga gcccgagcgg cgcgtggacc
     4261 ggctcgtctt ccgggtgctg ctttcgggcg gggcggtcgt ggacctggcg ctcgagaggg
     4321 cctcggggga gtggctactc gttggcatgg tggactgaga tgagaagcca ggatcccggt
     4381 caaggcttcg cgcacctcca cgtccggagt ggcttctcct acgggttcgg ggtggcgaca
     4441 ccagccgagc tcgccaccgc cgcctcggag atgggcatag aggcgctcgc cctcacggac
     4501 agggacggcc tctccggcat accgaggttc ttcgaggccg ccgcgggcgc gggcgtctcc
     4561 ccggtactcg gtgcggaggt cagcgtggat ggaggtggtc acctggtgct gctggccgac
     4621 agcatgacgg gctaccgctc cctctgccgg ctcatcaccc gctacaggtg cggctcggag
     4681 gaccggcgca aacccctctg cccgttccct ctgctcttgg agcacgccga gggcctcgtc
     4741 tgcctcacgg gcgcggtccc gttcgggcac gtgccgcgcc tggtgctctc cggcctgaca
     4801 gggacggcgg accgggtcct gcgcgcgctg cgggaggctt tcggagaggg tctctacgtc
     4861 gagctcacag acgaccggac ggcggggagc cggaggcgga tggggcgggt cacggagctc
     4921 gcccggcgca actgcctccc cgtgctcgcg acgaacgagg tagcctacct ggcgccagag
     4981 gaccacaagc tccacgacgt cgtcgtggcc acctcgaacc tgacagctct ccccggcccc
     5041 ggctaccgga caacagacca gctctacctc aagtcgccgg aacaggtgga gaagttgttc
     5101 cgcgatcgcc ctgaggccgg gggtaacgcg gcagaggtgg ccgagcgctg cgccggcgcc
     5161 gtcgacctgg gaggggagat ccacgtgcca tcggcccggc tgcgcggggg cgagacagct
     5221 cgtgggaagc tcgcccggct cgcgctgcgg ggggctaaag cgaggtacgg gcacgtcgag
     5281 gggcgggtgc gcgccaggct gctcagggag ctagggtgca tagaggaact cggcttcgcc
     5341 ccctacttcc ttctcgcgcg cgaggccgca gagatagcac gcgagaaggg ggtgcccgtg
     5401 acgggcaggg ggagcgcggc caactccctc gtctcctact gcctggggct cacacgccct
     5461 gagcccttct ccaaccgcct cttattcgag
//
LOCUS       DASTUV010000112         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_928237, whole genome shotgun sequence.
ACCESSION   DASTUV010000112 DASTUV010000000
VERSION     DASTUV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928237"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..106
                     /locus_tag="VFT03_02340"
     CDS             14..106
                     /locus_tag="VFT03_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847011.1"
                     /translation="MRKLNAQVDIEREDPEDVAAEYLQEKGIVE"
     gene            491..2155
                     /gene="hutU"
                     /locus_tag="VFT03_02345"
     CDS             491..2155
                     /gene="hutU"
                     /locus_tag="VFT03_02345"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU4847012.1"
                     /translation="MTDTQRAPTIRAPRGTEISCKGWHQEAALRMLMNNLDPEVAERP
                     EDLVVYGGTGKAARDWESFWAIVSTLQDLDDDETLLVQSGKPVAVFRTHAWSPRVLIA
                     NSLLVPDWAEWDTFRKLEKAGLTMYGQMTAGSWIYIGTQGILQGTFETFAAVAEQCFG
                     GTLRGRVCLTAGLGGMGGAQPLAITMNEGIALCVEVDPDRARRRVETGYLDVLANDVD
                     SALERAGEARREGVPLSIGIPGNAAEVFPALLESGYAPDVVTDQTSAHDALGGYVPAG
                     YTLEEAANLRESDPERYVSESRASMARHCAAMVGMRERGAEVFDYGNNLCAEAKLGGF
                     DGAFSYPGFVPAYIRPLFCEGKGPFRWAALSGDADDIAATDDAILDLFPDDDRLARWI
                     QQARDRVHFQGLPARICWLGAGSRHRAGLRFNELVADGTISAPIVIGRDHLDTGSVAS
                     PYRETEAMRDGSDAIADWPILNALLNTTSGASWVAVHHGGGVGIGKSIHSGAQVLADG
                     TEEGASRLERVLTNDPSIGVVRHADAGYEVAREAARRLGIRMPMFD"
BASE COUNT          388 a          763 c          740 g          337 t
ORIGIN      
        1 ggacgcggag accatgcgca agctcaacgc tcaggtggac atcgagcggg aggatcccga
       61 ggacgtcgcc gcggagtacc tccaggagaa gggcatcgta gagtaggtgc cagccccgct
      121 cttatgctgt gggggaaggg agaccgccgc atagagcgct ggtttgccgg cggggtgccg
      181 ctttcggccg tacgatcccc tgctgcgcaa ggtcctttgc cggcggtcgt gatcttccac
      241 ggcttggatg gcaacaagac ggacgacctg ctgtgggtcg ctctcccgct cgccgacgag
      301 acctcgaccc gcgctccgcg ccgggcgacg cgcacgacat cctcgatgga gaagtcgtcg
      361 cccgtgagcg cttctcgagg gcaagagagc caaagcgtac ctccgacgtc tggcagcata
      421 ctactcccct gccctggact tcctcaacgc ccggtgccag actgatcaac agcgcgggaa
      481 ggagccacca atgaccgata cgcagagagc cccgaccatc agggcgccga gggggacgga
      541 gatctcctgc aagggctggc accaggaggc cgctttacgc atgcttatga acaacctcga
      601 tcccgaggtc gcggagcgcc cggaggacct cgtcgtctac ggcggcacgg ggaaagcagc
      661 acgcgactgg gagagcttct gggccatagt gtccacgttg caagatctcg acgacgacga
      721 gaccctcctc gtgcagtcgg gcaaacccgt cgcggtcttc cgcacccacg cctggtctcc
      781 gcgcgtcctc atcgccaact cgttgctcgt ccccgactgg gctgagtggg acacgttccg
      841 aaagctggag aaggcgggcc tcacgatgta cggccagatg accgcggggt cctggatcta
      901 catcggcacc cagggcatct tgcaggggac ttttgagacg ttcgccgccg tcgccgaaca
      961 gtgcttcggc ggcaccctgc ggggacgcgt ctgcctcacc gccggcctcg ggggcatggg
     1021 cggcgcccag ccgctcgcta taaccatgaa cgaggggata gcgctctgcg tcgaagtaga
     1081 ccccgaccgc gcccgccggc gcgtagagac gggctacctc gacgtgctcg ccaacgacgt
     1141 agacagcgcc cttgagcgcg ccggggaagc gaggcgcgag ggcgtccctc tcagcatcgg
     1201 catcccgggc aacgcggcag aggtcttccc cgctctgctt gagagcggct acgcccccga
     1261 cgtcgtcacg gaccagacct cggcccacga cgctctgggc ggctacgtcc cagccggata
     1321 caccctggaa gaggcggcga acctgcgcga gagcgacccg gagcgctacg taagcgaatc
     1381 tcgcgcctcg atggcccgcc actgcgcggc gatggtaggc atgcgggagc gaggagcgga
     1441 ggtcttcgac tacggcaata acttgtgcgc ggaggcgaag ctgggcggct tcgacggggc
     1501 tttttcgtac cccggcttcg tccctgccta cataaggccg ctcttctgcg agggtaaggg
     1561 gccgttccgg tgggccgccc tttcggggga cgctgacgac attgcggcga cagacgacgc
     1621 gatcctggac ctctttcccg acgacgatcg tctcgcacgt tggatccagc aggccagaga
     1681 cagggtccac ttccagggcc tgcccgcgcg catctgctgg ctcggggctg gctcgcgcca
     1741 cagggcgggc ctgcgcttca acgagctcgt cgccgacggg accatctctg cccccatagt
     1801 catcgggcgg gatcacctcg acacgggaag cgtcgcctcc ccctaccgcg agaccgaggc
     1861 tatgagggat ggatccgacg ctatcgccga ctggcccatc ctgaacgccc tcctgaacac
     1921 caccagcggg gcgagctggg tcgccgtcca ccacggtggc ggagtcggga tcggcaagtc
     1981 catccactcc ggagcccagg tactcgccga cggcacggaa gaaggcgcca gccgcctcga
     2041 aagggtactc accaacgacc cttctatagg cgtcgtccgc cacgccgacg cgggctacga
     2101 ggtggcccgc gaggcggcac gcaggctggg tatccggatg ccgatgttcg actagggtga
     2161 gacgcaccct cctgcccgac ctgctcctcg acgcgcgcgg cgcccgcagc aacgtcgccg
     2221 tgacggtc
//
LOCUS       DASTUV010000113         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_774353, whole genome shotgun sequence.
ACCESSION   DASTUV010000113 DASTUV010000000
VERSION     DASTUV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774353"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /gene="yqeK"
                     /locus_tag="VFT03_02350"
     CDS             <1..561
                     /gene="yqeK"
                     /locus_tag="VFT03_02350"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU4847013.1"
                     /translation="LARKRLSDKRYGHTVRVSDTAEGLAQIHSLDPNRTRLAALLHDA
                     ARETEPEDYLRLADEWGLAVGELERKSPKLLHGPVAAELARRELGVEDEEVLEAIRVH
                     TVGAAGIGALALALYVADKIEPARDYPSVGRIRDLAREDLYEAAAESLRRAIGHNEGR
                     GKPIHPASREMLEWLEHTKNAAHRGI"
     gene            complement(514..1125)
                     /locus_tag="VFT03_02355"
     CDS             complement(514..1125)
                     /locus_tag="VFT03_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU4847014.1"
                     /translation="MLPPHELLNSAPSLNATTHAVFGVAALAGASLLAGTEPPAYAYP
                     VAVAAAWLPDVDNPRSTLGNGLSRMKSPALNLLTRPLSWALRTMSFTLVRTVGHRTLT
                     HSLLGVALFALPVWLILGGYPNLALALVAGYASHVFADALNTRGVPLLWPMGRPFRLL
                     PGGIRSGGAVEVAVALVALGFAGWELYLLYPAVRGVLGVLQPL"
     gene            1276..3156
                     /locus_tag="VFT03_02360"
     CDS             1276..3156
                     /locus_tag="VFT03_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847015.1"
                     /translation="MRALFVSILSCVFLAAFAVSAGSLPANAAPLPRPVVLVLVEDLS
                     WDDVNSEPDLQRAFRDGAAATLSVVQGTAPPDDPRFGYVFLGAGSRVDTRFLPEQLPT
                     DQDRIPDAFDGPARTVHPGSLGGALEEAGVQAAAVGDKARLVVMTPDGEVPRSYEDPI
                     AGLDAALEDGAGFVAVDAVDPRQAAQLVEATRETGAAVAVASPNGPKGTPNLTPFVFL
                     RPGSGGGLLYSPATRTEGLLTNADVAPTLLDALGAPVPPEMSGRAAEVRPGDVESAEL
                     LQRRLWLVEDKGFQVWGVVGVLWALALAVGTARRGRRGASWAVLALAALPAGALLAAA
                     FPVTDVLSVGALTALFAAGMTALSWRLSGSFAGAFAGALAGIALATAALVTLDAAAGG
                     PLERFSTIGYNPATGTRFYGVGNEYAAVLAGSLTLGLGVLTHRRRPPAVPLATVGAVA
                     VLALGLPTMGADVGGSLALGLAFGATVGLVRNDGWKGAVLWAAGGFALAAALFLASGL
                     LFPDVSHGSSAAAGGEDLYEVFVRKVALSLDSLLNPVLLLLTALGGAVTYGGWRQARG
                     TPLAAAIPGAVVAAVASGIFNDTSLIAALFALMYPALGALGVLVSKENAGPRRSFPRR
                     RI"
BASE COUNT          497 a         1127 c         1164 g          502 t
ORIGIN      
        1 ctcgcccgca agcgtctctc cgacaaacgc tacgggcaca ccgtgagggt gtctgacaca
       61 gcggagggcc tggcgcagat ccacagcctc gaccccaacc ggacgcgcct cgccgccctc
      121 ctgcacgacg ccgcacgcga gacggagccc gaagactacc tcaggctcgc agacgagtgg
      181 ggcctcgccg taggcgagct ggagcgcaag agcccgaagc tcttgcacgg acccgtcgcc
      241 gcggagctgg cgcggcggga gctcggcgtc gaggatgaag aggtactgga ggcgatcagg
      301 gtccacaccg tcggggcggc ggggatcggg gcgctcgctc tcgctctgta cgtggcggac
      361 aagatagaac ccgcccgcga ctacccgagc gtggggagga tcagggacct ggccagagag
      421 gacctctacg aagctgcagc cgagtcgctg cggcgcgcca tcggccacaa cgaggggcgc
      481 ggcaaaccca tccatccagc gagccgcgag atgctagagt ggctggagca caccaagaac
      541 gccgcgcacc gcgggatata gcaggtagag ctcccacccc gcgaaaccca gagccacgag
      601 cgccacggcg acctccaccg cgccgcccga gcgtatgccg ccgggtagga gcctgaaagg
      661 tctgcccatc ggccagagga gcgggacccc acgcgtgttc agcgcgtccg cgaagacgtg
      721 cgaggcgtag cccgcgacca gggctagagc gaggttcgga taccctccca ggatgagcca
      781 gaccggcagg gcgaacagcg ctacgccgag cagggagtgg gtcagggtgc ggtggccgac
      841 ggtccgcacg agggtgaagg acattgtcct cagagcccag ctcaagggcc gggtcaggag
      901 gttcagcgct gggctcttca tgcggctgag gccgttgcct agagtgctgc gcgggttgtc
      961 cacgtcgggc agccaggcgg ccgcgactgc cacggggtag gcgtaggccg gcggctctgt
     1021 cccggcgaga agagaagccc ccgccagcgc cgcgaccccg aagacggcgt gtgtcgtcgc
     1081 gttcaagctc ggggcggagt ttagcaactc gtgaggcggc agcaccagta tcgccgcttc
     1141 ccgtcagggt ccctctctca gatcggcgtc ctgacgttca cggccacgct cttgcaagag
     1201 catcgctcat cttcggggcg cggagccgcc tccgggaagc gcccgcgaat acccctctgc
     1261 taaactggtc ccgccgtgcg ggcgctcttc gtctcgatac tatcctgcgt tttcctggcg
     1321 gccttcgccg tctcggcggg ttctctcccg gccaacgcag ctccactccc gcgtcccgtc
     1381 gtcctcgtcc tcgtcgagga cctctcctgg gatgacgtga atagcgagcc ggacctccag
     1441 agggctttcc gggacggcgc ggcggcgacg ctctcggtag tccaggggac cgcacctccc
     1501 gacgaccccc gcttcggcta cgttttcctg ggcgctggct cgcgggtgga caccaggttc
     1561 ctgcccgaac agctacccac ggaccaggac cgcattccgg acgcgttcga cggtcctgcg
     1621 agaaccgtcc accccggttc tctgggcggc gccctcgaag aagccggggt tcaggccgct
     1681 gccgtgggag acaaagcgag gctggtagtc atgaccccgg acggcgaggt tcctcgcagc
     1741 tacgaagatc caatcgccgg cctcgacgca gccctggaag acggcgcggg cttcgtcgcc
     1801 gtggacgccg tggacccacg gcaggccgcg cagctcgtcg aggccaccag ggagacggga
     1861 gccgccgtcg ccgttgcttc acccaacggt cccaagggta cgccgaacct gacaccgttc
     1921 gtgttccttc ggcccgggag cggaggtggt ctcctctact cgccggcgac ccgcacggaa
     1981 gggctcctga cgaacgcgga cgtcgctccg acgctgctcg acgctctcgg ggcaccggta
     2041 ccacccgaga tgagcggtcg ggcggcggag gtacgccctg gggacgtgga gagcgcggag
     2101 cttctgcagc gcaggctgtg gctcgtcgag gataaagggt tccaggtgtg gggtgtggtg
     2161 ggagttctgt gggcacttgc cctggcggta gggacagcgc gacgcggaag gcggggggcc
     2221 tcctgggccg tgctcgcact ggcggcgctg ccagccgggg cgctgctcgc agccgccttc
     2281 ccggttacgg atgtgctgtc cgtcggcgcg ttgaccgcgc tcttcgccgc tgggatgacg
     2341 gctctctcct ggaggctctc cggctcgttc gccggggcgt tcgccggggc gctcgccggg
     2401 atagccctgg cgacggcggc tcttgtgacc ctggacgcag cggcgggagg accgctcgaa
     2461 cgattctcca ctataggcta caacccggcc acagggacgc ggttctacgg ggtgggcaac
     2521 gagtacgccg cggtcctggc cggtagcctg acgctgggcc ttggcgtgct cacgcaccgg
     2581 aggcgacctc cggcggtgcc gctcgcaacg gtcggcgcgg tcgcggtgct cgcgctcggg
     2641 ctcccgacga tgggcgccga cgtcggcggc tcgttggccc tcggcctcgc gttcggggcg
     2701 acggttgggc tcgtgcgcaa tgacgggtgg aagggtgcgg tcctgtgggc ggcaggcggg
     2761 ttcgcgctcg cggcggcgct gttcctggcg agcgggctcc tcttccccga cgtctcgcac
     2821 ggctcgagtg ctgcagccgg cggcgaggac ctctacgagg tgttcgtccg caaggtggca
     2881 ttgagcctgg actcgctgct caacccggtt ctgctcctat tgacagccct cggcggcgcc
     2941 gtgacctacg gcggctggcg ccaggcgcgc gggacgcctc tagccgccgc gatacccggc
     3001 gcagtcgtag cggccgtcgc ctcggggatc ttcaacgaca ccagcctcat cgccgccctc
     3061 ttcgccctca tgtacccggc cctgggcgcc ctcggggttc tggtctcgaa ggaaaacgcc
     3121 ggaccgcggc gatccttccc gagaaggaga atttaagcac gcctgcatcg caggcgtgct
     3181 gacaagctgc cgaaggcagc gtgctattcc gcgacacgct ctccgagctc ggccatcagg
     3241 ccgtcgaagt cgagggggcg cctcgtgaag gacaattcgc cattcctcct
//
LOCUS       DASTUV010000114         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1703107, whole genome shotgun sequence.
ACCESSION   DASTUV010000114 DASTUV010000000
VERSION     DASTUV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703107"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..826
                     /locus_tag="VFT03_02365"
     CDS             62..826
                     /locus_tag="VFT03_02365"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU4847016.1"
                     /translation="MAVDVGNTQTVLGLIDGKEPRGEWRITTEAHRTADELAVIFAGL
                     LALEGLSPDEVSAMIVSSVVPGLTRSYRNLASEVFGVPYYVVTAEMETGLDNRYDDPG
                     AVGADRIVNAVAAGRHYGFPAIIVDIGTATTVEAVDGEACYLGGAILTGLYVSLDALV
                     SKTAKLPSVDLEEEPPRVIATNTPDSIRSGFIYGYAGAVDALIRRSRDELGEDGVKVI
                     ATGGPAGVIVPYCREIQEFDPDLTLKGLLALYEMNA"
     gene            1083..1571
                     /gene="greA"
                     /locus_tag="VFT03_02370"
     CDS             1083..1571
                     /gene="greA"
                     /locus_tag="VFT03_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU4847017.1"
                     /translation="MTDKNQELVTPEGLEKLQQELAYLTETRRKEVADRIRQAREFGD
                     ISENSEYDEAKNEQGLLERRIGELQRRVRNVKVVDPSEVEEDAVDLGTRVTLRIVGRK
                     GQERTFQIVGANESDPTSGKLSHASPVGRAVLKRKVGEKVMVSTPRGATEYEIVNVEA
                     AS"
     gene            1636..>3005
                     /gene="lysS"
                     /locus_tag="VFT03_02375"
     CDS             1636..>3005
                     /gene="lysS"
                     /locus_tag="VFT03_02375"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU4847018.1"
                     /translation="MSETGRSTGIHIPGWAHRVADSLGAGPHVVVSGISVSGNIHAGN
                     LREVLVAEAVANALRQRGEEVRFIFHADTIDPLRKIAPGVPESYEEYIGHSLSHIPDP
                     EGCHVSYAEHFLAPFEEALREMEMDVEVLRSHELYERGVYTEVTSEAIEHTDELREIL
                     QDVTNRKMPENWSPYLPRAASGKLTGMRVLEHLPEQTSVVFADEDGWQDSADYSRGEG
                     KLGWRVELAARWKALGVTFEPFGKDHTSRGGSTDTADRMSREVFHYPVPGRYEYEWIG
                     LKGRGDMSSSRGIVLLPEDLLRIMPPGAVRHIILGRDPARRFDIDLEGGFPRFMDEYR
                     AEAGEPYVPFTHLVTVAQTVGEDTDAAAQMLRRGGYEEAIGHGLANDLYYARNWAAEW
                     APESMRLGLLDPAESKEAAAALDDEQREYLREVSARLRAEMDGEAIQDVLYSTAIERG
                     LKPKRA"
BASE COUNT          589 a          922 c          993 g          501 t
ORIGIN      
        1 tgcttgccgt agcggaagag ggaggaatct tgacgttcgg ggaggctgaa gaactttgtt
       61 gatggccgtg gacgttggga atacacagac tgttctgggg ctcattgatg gcaaggagcc
      121 gcggggagag tggcgtatca cgacggaggc gcacaggacg gctgacgagc tcgccgtcat
      181 cttcgcgggg cttctggcgc tcgagggtct gagccccgac gaggtgagtg cgatgatagt
      241 ctccagcgtc gtgcctggtc tcacccgttc ctatcgtaac ctcgcctctg aggtcttcgg
      301 ggtaccctac tacgtggtaa ctgcggagat ggagaccggc ctcgacaacc gctacgacga
      361 cccaggggcc gtcggggccg acaggatcgt caacgccgtg gcggcaggga ggcactacgg
      421 gttccccgcg atcatcgtcg atatcggcac ggcgacgacc gtcgaggccg ttgacggcga
      481 agcatgctat ctcgggggcg ccatactgac aggcctctac gtttcgctgg acgcgctcgt
      541 ctccaaaacg gccaagcttc ccagcgtgga cctcgaagaa gaaccaccga gggttatagc
      601 caccaatact ccagactcta tccgcagcgg tttcatctac ggctacgccg gcgctgtcga
      661 cgccctgatc aggaggtccc gtgacgaact gggcgaggac ggcgtcaagg tcatcgccac
      721 aggtggtccg gccggggtca tagtgcccta ctgccgcgag atacaggagt tcgaccccga
      781 cctcaccctc aagggcctcc tcgccctcta cgagatgaac gcttgagagc atttcagcaa
      841 ttcagcacgc gcctttgcgg gcgcttgtca gcacgtcgcc cggcttcgcc ggacgacttg
      901 tcagcatttc agctagtcag ccagtgcaag ccctgctgaa atgctgaaag cgagggcctt
      961 taggtccgaa gcgtgctgac aagcgaaggc gaagcctgag catggtgact tgctgaaacg
     1021 ctggctgcgg cagcgtttcc tcgttgacac cctttgcacc cgcccttata atctcgcggc
     1081 ttatgaccga taagaaccag gagctggtta ccccggaagg gctcgagaag ctccagcagg
     1141 aactcgcata cctgaccgag acgcgtcgca aggaggtcgc cgaccgcatc cgccaggctc
     1201 gtgagttcgg ggacatctcc gagaactccg agtacgacga ggcgaagaac gaacagggtt
     1261 tgctggagcg ccggatcggt gagctccagc gcagggtacg caacgtgaag gttgtcgacc
     1321 cttcggaagt cgaggaagac gcggtcgacc tcggtactcg ggtaaccctc aggatcgtcg
     1381 gcaggaaggg acaggagcgg acgttccaga tagtcggcgc caacgagtcg gacccgacga
     1441 gcgggaaact cagccacgcc tccccggtgg gccgggcagt actcaagagg aaggtggggg
     1501 agaaggtcat ggtctccacc ccccgcggcg cgacggagta cgagatcgtc aacgtcgagg
     1561 cggccagtta gggcgccggg agcgcacggc ccacttctcc ccgattccaa ccaccgaacc
     1621 cagttggagt accgcatgag cgagaccggc aggtcgaccg gcattcacat acccggctgg
     1681 gcccataggg tcgccgattc tctgggcgcg gggccgcacg tggtcgtgag cggcatcagc
     1741 gtctcaggta acatccacgc tggcaacctg cgcgaggtcc tagtcgccga ggcggtggcg
     1801 aacgctctgc ggcagcgagg cgaggaggtt cgctttatct ttcacgcgga cactatcgac
     1861 ccgctgcgca agatagcccc aggggttccc gagagctacg aggaatacat cggccacagc
     1921 ctctcgcaca taccggaccc cgaaggctgc catgtctcct acgccgagca ttttctcgcg
     1981 ccgttcgaag aggcgctcag agagatggag atggacgtag aagtgctccg ctcgcacgag
     2041 ctgtacgagc ggggcgtcta caccgaggta accagcgagg ccatcgagca cacggacgag
     2101 ttgcgtgaga tcctccagga cgtcacgaat cgaaagatgc ccgagaactg gtcgccctac
     2161 ctgccccgcg cggcatcggg gaagcttact gggatgcgcg ttctggaaca cttgccggag
     2221 cagaccagcg tcgtcttcgc ggatgaggac ggctggcagg acagcgccga ttactccagg
     2281 ggcgagggca agcttggctg gcgggtcgag ctcgcggccc ggtggaaggc gctcggggta
     2341 accttcgagc ctttcggcaa ggaccacacc agtcgcggtg gctcgaccga caccgctgat
     2401 cggatgtccc gcgaggtctt ccactacccg gtgccgggcc gctacgagta cgagtggata
     2461 gggctcaagg gccgcgggga catgagcagc tctcggggta tcgtgctcct gccggaggat
     2521 ctcctcagga taatgccccc cggcgccgtc aggcacatca tcctcggacg cgaccccgcc
     2581 cgtcgcttcg atatcgacct ggagggcggc ttcccgcgct ttatggacga ataccgcgca
     2641 gaggccggcg agccctacgt ccccttcacc catctcgtca ccgtcgccca gactgtcggt
     2701 gaagacacgg acgccgccgc acagatgctg cgtcgtggcg ggtacgagga ggcgatcgga
     2761 cacgggctgg caaatgacct ctactacgcc cgcaactggg ccgcggagtg ggctcccgag
     2821 tcgatgcggc tgggattgct cgacccggct gaatcgaagg aggccgctgc agcactcgac
     2881 gacgagcagc gcgagtacct gcgcgaggta tcggcaaggc tccgggccga gatggatgga
     2941 gaggctattc aggacgtgct gtattcgact gccatcgagc gcgggctcaa gcccaagagg
     3001 gcgtt
//
LOCUS       DASTUV010000115         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_738626, whole genome shotgun sequence.
ACCESSION   DASTUV010000115 DASTUV010000000
VERSION     DASTUV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738626"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFT03_02380"
     CDS             <1..488
                     /locus_tag="VFT03_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU4847019.1"
                     /translation="VATAGPIQSKRTGRYCDGIITVGAADEKIRMLMQKFEEGAREEG
                     KDPGRMPRMIQLHVSWAEDQQQAEEQAVKEWPNGGMPFPKADIRNPEDFAAMAKQVSI
                     EHFENRVLTSADLDEHLEHVQHYVDLGFDEVYVHNVGRNQEEFIRAYGERVIPKLRWP
                     G"
     gene            807..2111
                     /locus_tag="VFT03_02385"
     CDS             807..2111
                     /locus_tag="VFT03_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU4847020.1"
                     /translation="MNPEELLARHKATLPSWMPLYYERPMELERGEGFRVWDSEGNEY
                     LDFFGGIVTAISGHNVPEITGAIKDQVDKILHTSTLYLIENQVRLAEKLIELAPISGE
                     KKAFFTVSGSEANEAALLFATRYRNSSEVIALRSSYHGRSFATMGITGQSTWSPTSRS
                     ALDVAYAQNPYPLRCGFCKHEGRCNLRCADDVRAVIEEETTGNVAAFIAEPIQGVGGF
                     IEAPPGYFERVKEILDERGVLLIADEVQTGFGRTGSHFWGIEAFDVEPDLVVIAKGIG
                     NGLAIGGVVGRAEVVESVSPHLHLSTFGGNPVSTTGALANLNYILDNDLQSNASEVGG
                     HLRERLLKMAENHPVVGEVRGRGLMLALELVGEGLTPDPQAANRFLEACRTRGLLVGK
                     GGLKANTIRISPPLTITREAADEAADILEEALAEVGTTERVG"
BASE COUNT          424 a          649 c          707 g          340 t
ORIGIN      
        1 acgtagcgac cgctggcccg atccagagca agaggactgg caggtactgc gacgggatca
       61 tcaccgtcgg ggcggcggac gagaagataa ggatgctcat gcagaagttc gaggagggcg
      121 cccgcgagga gggcaaggac ccgggtcgta tgccgcgaat gatccagctt cacgtctcct
      181 gggccgaaga ccagcagcag gccgaggagc aggcggtgaa ggaatggcca aacggcggga
      241 tgccgttccc caaggccgac atccgcaacc cggaggactt cgcggcgatg gccaagcagg
      301 tctccatcga gcacttcgag aaccgcgtcc tcacctccgc cgacctggac gagcacctcg
      361 agcacgtcca gcactacgtg gaccttggct tcgacgaggt ctacgtccat aacgtcgggc
      421 gcaatcagga ggagttcatc agggcctacg gagagcgcgt catccccaag ctccgctggc
      481 cgggctgaac ccgttttaca acaaaccttt gctttgctta tatactgcct ttcgaggaaa
      541 gggaggcgac gacggagagt cggagagtta tctcgcggcc tgtatacggt cgagtcaccc
      601 gggaacaaag aggttcggtg gcgggtggaa cctttctccg cgcggccata gctggatctc
      661 agacacgtct ccatagtccg ggggtcgggc ttcggctgct cacgcgctgc ttccttgcac
      721 cggaaaggag taaagacttt gagtgctttc tacaggagta agcgtcgtaa ccgcaggcgg
      781 cagcttccgg acgtggaggt gaaggcatga accctgagga gcttctggcc aggcacaagg
      841 ccacgctgcc atcgtggatg ccgctctact acgagagacc catggagctg gagcgcggcg
      901 aggggttcag ggtctgggac tccgagggta acgagtacct ggatttcttc gggggaatcg
      961 tcacggccat aagtgggcac aacgtgcccg agataaccgg ggccataaag gatcaggtag
     1021 acaagatcct gcacacctcg acgctgtacc tgatcgagaa ccaggtccgc ctcgccgaga
     1081 agctcataga gctcgctccg atctcgggtg agaagaaggc gtttttcacc gtgagcggga
     1141 gcgaggcgaa cgaggcggcg ctactcttcg ccacgcgcta ccggaactcg agcgaggtca
     1201 tagccctgag gtcctcatac cacggtcgct cgttcgctac gatgggcata acgggccaga
     1261 gcacctggag cccgacctcc cgctccgcgc tggacgtggc gtacgcgcag aatccctacc
     1321 ccttgcgctg cgggttctgc aagcacgagg gtaggtgcaa cctgcgctgc gcggacgacg
     1381 tgcgagcggt catagaggag gagaccacgg gcaacgtcgc cgctttcatc gccgagccca
     1441 tacaaggggt gggcgggttc atcgaggcgc cgccgggcta cttcgagcgg gtaaaggaga
     1501 tcctggacga gcgaggcgta ctcctgatcg ccgacgaggt ccagacaggc ttcgggcgca
     1561 ccggctccca cttctggggc atcgaggcct tcgacgtcga gcccgacctt gtcgtcatag
     1621 cgaagggtat cggcaacgga ctcgccatcg ggggcgtcgt gggccgggcc gaggtcgtcg
     1681 agtccgtcag cccccacctt cacctctcga cgttcggcgg taacccggtc tctacgacgg
     1741 gcgccctggc aaacctgaac tacatcctgg acaacgacct ccagagcaac gccagtgagg
     1801 tcggcggcca cctcagggaa cgcctcctga agatggccga gaaccaccct gtcgtcggcg
     1861 aggtgcgcgg gcgcggcctg atgctcgccc tcgagctcgt cggcgaggga ctaacacccg
     1921 atccgcaggc cgccaatcgc ttcctcgagg cgtgccggac gcgcggcctg ctcgtcggca
     1981 agggcggcct caaggcgaac acgatccgga tctcgcctcc gctcacgata actcgtgagg
     2041 cggccgacga ggcggctgac atcctggagg aggcgctcgc agaggtcggg acgacggaga
     2101 gggtgggctg atgcagactc
//
LOCUS       DASTUV010000116         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1499992, whole genome shotgun sequence.
ACCESSION   DASTUV010000116 DASTUV010000000
VERSION     DASTUV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499992"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..611)
                     /locus_tag="VFT03_02390"
     CDS             complement(135..611)
                     /locus_tag="VFT03_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU4847021.1"
                     /translation="MSEETPRRQTKVLDKQRLRSFVLGGVAGLLAGILLAPRSGRELR
                     GSIRDRAGEARERSRESLFEAQERMQERISEVREGPDRRTGAPGPPAEPPEAAPRPQT
                     RPFLRDVSRGEDEPAERGGERSEELRRKVRETRARLRSNLDRPGGGGEDRDLGPRT"
     gene            complement(613..1614)
                     /locus_tag="VFT03_02395"
     CDS             complement(613..1614)
                     /locus_tag="VFT03_02395"
                     /inference="COORDINATES: protein motif:HMM:NF003259.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HEU4847022.1"
                     /translation="MSAPGGAMEERDIMVVRADRETPFSRGILAQTLAQAGARPELAH
                     QIASEVRAELLAEERYMVEEEEVLARVRDKLIKKDAIVVGRLDKWRILRESTEPIVVL
                     IGGATGVGTSTLAADVARRLNIQSVIGTDSIREVLRRAISPDLLPILHKSSYEIKRED
                     VRVPVEEEPVLFGFREQASRVAVGVEAIVDRGLKEGTNLVIEGVHLVPEVILERYRDH
                     PNVCPLVVYLSDEHVHRSRFYIRALGTAMRRPAEEYIAYFKEIRQIHDYIVQSASRTG
                     VQKVENVSIEHTSDAAVEIVTNRVSGIAERARRHSSAFLLDTATPRHGVPITRAKSS"
     gene            complement(1621..2214)
                     /locus_tag="VFT03_02400"
     CDS             complement(1621..2214)
                     /locus_tag="VFT03_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEU4847023.1"
                     /translation="MLGMEILLALGIGIGFASVAGVRAFLPLAALFAALGLFEPTSPY
                     VDVDGGGWWWSTFGVLAALALVESVLDKIPALDRAFNYAMVPVRIASGAVLFAAALGM
                     GLDPGSAPWLVAGGAIAGVVAVAKVILRPPAKAVSAGVSPAFLSFFEDVVAMVGGALG
                     FFVPLVPLAPVAFLLYFYYRVRRRRGRKYGGLRILGD"
BASE COUNT          394 a          842 c          714 g          418 t
ORIGIN      
        1 gttagcctcg cggaaccaat ccacggctga gcacgcccct tcggtccagt gcgagatcgc
       61 cgcctcgagc ccaggtatct ccccggctcg ttccgcggag atctcctcta cagtggggac
      121 gaaagaagtg ggtctcacgt acgcggtccc aggtcgcggt cttccccgcc gccccctggc
      181 ctgtcgaggt tcgacctgag gcgggcgcgg gtctcgcgga ccttcctccg cagctcctcc
      241 gacctctcgc cacctctctc ggcgggctcg tcttcgccgc gggagacgtc tcgcaggaag
      301 ggtcgcgtct gcggccgggg cgcggcctca ggaggctccg cagggggtcc cggcgcaccg
      361 gtgcgccggt cagggccctc gcgcacctcg gagatacgct cctgcatccg ttcctgagcc
      421 tcgaagaggg attcgcggct ccgctcgcgg gcctcccccg cacggtccct gatcgagccc
      481 ctcaactccc tgccgctgcg gggggcgagc agtataccgg cgaggagccc ggccacgccg
      541 ccgagcacga agcttctcag ccgctgcttg tccagcacct tcgtctgcct cctgggcgtt
      601 tcctcgctca ttctaactgc ttttcgctcg cgtgatcggg accccgtgcc tcggcgtggc
      661 ggtatctagg aggaaagccg aagagtgacg gcgagccctc tcggcgatcc ccgatacccg
      721 gttcgtgacg atctcgactg cggcgtccga ggtgtgctct atggagacgt tctcgacctt
      781 ctgcacgccc gtgcgggagg cgctctggac tatgtagtcg tggatctggc gtatctcctt
      841 gaagtaggcg atgtactcct cggccggacg gcgcatcgcc gtcccgaggg cgcggatata
      901 gaagcgcgag cggtgcacgt gctcgtcgga gaggtacacc acgagcgggc aaacgttcgg
      961 gtggtccctg tagcgctcca ggatgacctc cgggaccagg tgcacgccct cgatcacgag
     1021 gttcgtcccc tccttgaggc ccctgtcgac gatcgcctcg accccgacgg cgacccgcga
     1081 ggcctgctcc ctgaagccga agaggaccgg ctcctcctcg accggcaccc tgacatcctc
     1141 gcgctttatc tcgtaagagc tcttgtggag tatggggagg aggtcggggc ttatggcgcg
     1201 cctgaggacc tcgcggatcg agtccgtccc tatgacgctc tggatgttga ggcgcctggc
     1261 gacgtcggcg gcgagggtgc tcgtccccac ccccgtcgcc ccgccgatga gcaccactat
     1321 aggctcggtg gactcgcgaa ggatgcgcca cttgtccagc cggccgacga ctatggcgtc
     1381 cttcttgatg agcttgtcgc ggaccctggc cagtacctcc tcctcctcga ccatgtagcg
     1441 ctcttcggcc agtagctcgg cgcggacctc gctcgctatc tggtgcgcca gctcgggcct
     1501 ggcgcccgcc tgggccaggg tctgcgccag gatgccacgg gagaagggag tctcccgatc
     1561 cgccctgaca accataatgt cccgctcttc catagcaccc ccaggagcgc tcatacgctc
     1621 ctagtcgccc aggatgcgga ggcccccata cttcctcccc cgcctcctcc tcacccgata
     1681 ataaaagtat agtagaaacg ccaccggcgc cagtggcacc agcggcacga agaagcccag
     1741 agctccgccc accattgcca cgacatcctc gaagaagctc agaaatgccg gcgagacccc
     1801 agccgatacc gccttagccg gcggacgaag tatcaccttc gccacggcca cgacgccggc
     1861 tatcgcgcct cctgcgacga gccatggcgc tgatcccgga tcgagaccca tcccgagcgc
     1921 cgccgcgaac agcacagccc cagacgcgat ccttaccggc accatcgcgt agttgaaggc
     1981 gcggtcgagc gccgggatct tgtccagtac gctctccacc aacgcgagcg cagcgaggac
     2041 cccgaaagtc gaccaccacc atccgccgcc atcgacgtcc acatacggcg aagtcggttc
     2101 gaagagcccc agcgcggcga acagtgctgc cagcggcagg aaggccctca cccccgcgac
     2161 ggaggcaaac ccgatgccga tgccgagtgc gagaagaatc tccatcccga gcattatatc
     2221 ttagaagctg cggtcgcccg ttcaacttgg ggctctacct gagctatact ctttcctgtt
     2281 ctacggtgta acgttcctag aaatcctgga ggattttctt gtcgcaaacc gtttcgatgg
     2341 agaagatagt cgcgttctgc aagcgccg
//
LOCUS       DASTUV010000117         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_559759, whole genome shotgun sequence.
ACCESSION   DASTUV010000117 DASTUV010000000
VERSION     DASTUV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559759"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFT03_02405"
     CDS             complement(<1..396)
                     /locus_tag="VFT03_02405"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEU4847024.1"
                     /translation="MVRAEVRLRELEAMVAPYGSALVAFSGGVDSSLALAIAARALPN
                     NRVLAVTSNNETYLPSELDLARGFAASLGVEHLVVNTRELDDPNYASNPTNRCYFCKS
                     TLYSDLSRLAEERGYGCVIDGANKDDEGDY"
     gene            complement(389..970)
                     /locus_tag="VFT03_02410"
     CDS             complement(389..970)
                     /locus_tag="VFT03_02410"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU4847025.1"
                     /translation="MASAQNPYELPNDLPVPEDDGAADHLPGMRLPSVPLASTSGEIV
                     DLSALPGRTVVYCYPMTGRPDRSLPAGWDEIPGARGCTPQSCSFRDHHAELRSLGASV
                     FGSSTQDTEYQREAATRLHLPFALLSDSDLAFAGALGLPTFEVDGMVLLKRLTLVIDE
                     GWIEKVFYPVFPPDRSAEEVVEWLEDPGRVSDG"
     gene            complement(977..2218)
                     /locus_tag="VFT03_02415"
     CDS             complement(977..2218)
                     /locus_tag="VFT03_02415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU4847026.1"
                     /translation="MENGRPAAYITGVGVVSPVAIGRKRFWSSLLANESGARPVTLFN
                     PEGFEVRIAAECTEFDANDFMDRKLAARTDRFAQMGLASAKLALEDAGAWDELNQRPE
                     RVGLILGSGIGGVMSMETTQATIDSRGPARVNPFSVTKIMPNSAAAHIAIQLGVQGPS
                     STGALACSCGTDMMGLGYDLIRRGDADVVICGASEATITPVIVAGFIAMRAMSRRNDD
                     PEGACRPYDEGHNGFLIGEGSAVVVLESAASVERRGAEPYAKMKGVGRTTDAYNITDP
                     DPKGLGVLRAMQLAVNNAGLEGERIGYINPHGTGTVAGDGPESRAMAEINPNTRVSAT
                     KSTLGHSLGASGAIETAICALALKEQTIPPMRNLSKLAEDCAELDYVVDEPRDAPDLE
                     AALCANLGVGGHNVAIVLEKV"
     gene            2343..>2629
                     /locus_tag="VFT03_02420"
     CDS             2343..>2629
                     /locus_tag="VFT03_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847027.1"
                     /translation="MMFYAATALFVANFALGVLVQFGIVDTKPFRWLHHALFFAVFAS
                     AALAVAVGFLQGAPYRWALLPVLALYLVLPRVRAGTRGHAALACCAMIFYAT"
BASE COUNT          382 a          890 c          858 g          499 t
ORIGIN      
        1 gtagtcgccc tcgtcgtcct tgttcgcgcc gtctatgaca cagccgtagc cgcgctcctc
       61 tgcgagcctg ctcaggtcgg aatacagcgt gctcttgcag aagtagcagc ggttggtggg
      121 gttgctcgcg tagttggggt catcgagctc ccgcgtattc actaccaggt gctccacccc
      181 cagagaagcg gcgaacccgc gcgccaggtc cagctccgag ggtaggtacg tctcgttgtt
      241 cgaggtgact gcgagaaccc gattattggg tagcgcccgc gcggcgatcg ccaacgcgag
      301 cgaggagtcc accccgcccg agaacgccac cagcgcgctg ccatatggag caaccatggc
      361 ctcgagttcc ctcagccgca cttcggctct aaccatcgct caccctcccg ggatcttcga
      421 gccactcgac gacctcctcc gcgctcctgt ccggcgggaa gacggggtag aagacctttt
      481 cgatccaccc ttcgtcgatg accagcgtca gcctcttcaa tagaaccatg ccatcgacct
      541 cgaaggtcgg caggcccagc gcccccgcaa aagccaggtc cgagtcgctg aggagcgcga
      601 agggcaggtg tagccgcgtg gccgcctccc gctggtactc cgtatcctgc gtgctcgagc
      661 cgaacacgct ggcgcccagg gaccttagct ccgcgtgatg gtcccggaag gagcacgact
      721 gcggggtgca gccgcgggcg cccgggatct catcccagcc tgccggtagg ctcctgtccg
      781 gtcttcccgt catagggtag cagtagacga cagttctccc tgggagtgcc gacaagtcga
      841 cgatctctcc cgacgtcgac gccaggggga cggaagggag gcgcatcccc ggcaagtggt
      901 ccgccgctcc gtcgtcctcg gggacgggaa ggtcgttggg cagttcgtag ggattttgcg
      961 cgctcgccac ggtcttctag accttctcca gcacgatggc aacgttgtgg ccgccgacgc
     1021 cgaggttcgc gcacagggct gcctcgaggt cgggggcgtc gcgcggctcg tccacaacgt
     1081 agtccagctc ggcgcagtct tcggccagct tgctcaggtt gcgcattggg gggatcgtct
     1141 gctcctttaa ggccagggcg cagatggcag tctcgatggc gccgctcgct cccagggagt
     1201 ggcccagtgt ggacttcgtc gccgagaccc tggtgttcgg gttgatttcg gccatagccc
     1261 tggattcggg gccgtcgccc gcgacggtac cggtaccgtg cgggttgatg tagccgatcc
     1321 gctccccttc gagccctgcg ttattcacgg cgagctgcat ggccctcagt acgccgaggc
     1381 ccttcgggtc ggggtcggtt atgttgtacg cgtcggtggt gcgcccgacg cccttcatct
     1441 tcgcgtaggg ctcggcgccg cggcgctcga cggaggcggc gctctcgagg accacgaccg
     1501 cggagccctc tccgatcagg aacccgttgt ggccctcgtc gtaggggcgg caggcccctt
     1561 cggggtcgtc gttgcggcgg ctcatcgcgc gcatcgcgat gaacccggcg acgatgacgg
     1621 gggtgattgt agcctccgag gcgccgcaga tgacgacgtc cgcgtctccg cggcggatga
     1681 ggtcgtagcc aaggcccatc atgtccgttc cgcagctgca cgccagagcg ccggtggaag
     1741 agggtccctg cacgccgagc tggatggcta tgtgcgcagc ggcggagttg ggcatgatct
     1801 tggtgaccga gaacgggttg accctggctg gacccctgct gtctatggtc gcctgggtcg
     1861 tctccatgct catcacgccc ccgatgccgc tgccgaggat gagacccacc cgttcgggcc
     1921 gctggttcag ttcgtcccac gcgccggcgt cctcgagcgc gagcttggcc gaggcgagac
     1981 ccatctgggc gaaccggtcg gtcctggccg ccagcttgcg gtccatgaag tcgttcgcgt
     2041 cgaactccgt gcactcggcc gcgatcctca cctcgaagcc ctcggggttg aagagcgtta
     2101 cggggcgggc accgctctcg ttcgccagta gcgagctcca gaaccgcttg cgcccgatcg
     2161 ccacgggact gactacccct acccccgtta tgtaagccgc tgggcgtccg ttctccatct
     2221 cgccccctag caaaaacgcc tcactgcaag tccatcgaac tacgaatctt aacatcaccg
     2281 atccgggccg acgcgcccgg ccctatgcta taacctccgg ggatgatttc gaggatgacg
     2341 gcttgatgtt ctacgcagcg acggcgctct tcgtggccaa cttcgcgctc ggggtgctgg
     2401 tgcagttcgg gatcgtggac accaagcctt tccgctggct gcaccacgct ctcttcttcg
     2461 cggtcttcgc ctccgccgcg ctcgctgtcg cggtggggtt cttgcagggg gcgccgtacc
     2521 gctgggcgct cctgccggtc ctggcgctct atctcgttct gccgcgggta cgggcgggca
     2581 cgcgggggca cgcggcgctg gcctgctgcg ccatgatctt ctacgctac
//
LOCUS       DASTUV010000118         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1130766, whole genome shotgun sequence.
ACCESSION   DASTUV010000118 DASTUV010000000
VERSION     DASTUV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130766"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFT03_02425"
     CDS             complement(<1..203)
                     /locus_tag="VFT03_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847028.1"
                     /translation="MSDTYDVVIVGSGPAGYTAALYAARANLDVLVFQGFEVGGQLML
                     TSDVENYPGYRDGVMGPEMMDDF"
     gene            391..612
                     /locus_tag="VFT03_02430"
     CDS             391..612
                     /locus_tag="VFT03_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847029.1"
                     /translation="MAEREREAHGKPEPERYFAEYVPLPGGEGAALVALQEAINEHAA
                     HSWKLLSVAADPTSQGVFMVWDTTGFFSG"
     gene            649..1137
                     /locus_tag="VFT03_02435"
     CDS             649..1137
                     /locus_tag="VFT03_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847030.1"
                     /translation="MLRGAALLLFAALAAVLVWRAGEGPVSALETPEVRPAPGLFDER
                     RREASEVLASVRVGSFERPEEVPGYELLEEISTEADGARSAQLIVDTRSRGREDFTLI
                     TRDLKARYAGYDVVSVEFTDTSEVLDYHGGAIIVNTALGARNAGFYYAPPSEGYLVRV
                     VE"
     gene            complement(1172..2437)
                     /locus_tag="VFT03_02440"
     CDS             complement(1172..2437)
                     /locus_tag="VFT03_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU4847031.1"
                     /translation="MQSKSLVRDPGLAPDGHRKIEWAARHSPVLNRVRDTYLKDGTFE
                     GLGVGVALPIEAKTAYLTVVLAEAGAEVAVASPGPFFVQDDVAAALAECGVTVYASSA
                     SPPEEADRELERVLDTAAGDREAVLIDDRAGLVRLAHTTRRTLLGNIRGASEETTSGV
                     AKFRAMDNEGILEFPVIAANDARCKYRFDNRYGTGQSTITAIMQNTNLMVGGKRVVVL
                     GYGWCGKGIARYAAGLGARVTVSEVDPVHGLEAYADGFDVLPALDAAEIGEVFITATG
                     STDVLTAAHFERMRDGAILANAGGVDVEIDVEGLRRASSDVREVRRNVEEFAMADGRK
                     LHLIGRGMVVNLTAGDGHPVEIMDLTFAVQALCAHHLANNSRTMENGVHLLPNEIDDE
                     VARMKLDAVGMGVDSLTPEQEGFLASWRE"
     gene            complement(2679..3689)
                     /locus_tag="VFT03_02445"
     CDS             complement(2679..3689)
                     /locus_tag="VFT03_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU4847032.1"
                     /translation="MDFGVTLQTDPPAWRVVELARKAEALGFTYAWTFDSHILWQEPY
                     VIYSQILSATESMIVGPLVTNPATRDPSVTASTFATLNDMFGNRTICGIGRGDSAQRV
                     LGRKPTRLAEVEEAMHVIKELAEGREIDYDGTKVRIPWVSNGKLDVWMAGYGPKALNT
                     IGRKADGFILQLADPVILEWTMRHVHDAATEAGRDPDEVKVCVAAPAYVGDDLAHQRD
                     QCRWFGGMVGNHVADLVSRYGEDGEIPHALTDYIRAREGYDYGHHGKADNLSTNFVPD
                     NIVDRFCVLGTVEDHVAKLNELKDLGVDQFNIYLMHDAMEETLEAYGEEIIPALGLKT
                     AR"
     gene            complement(3706..4071)
                     /locus_tag="VFT03_02450"
     CDS             complement(3706..4071)
                     /locus_tag="VFT03_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4847033.1"
                     /translation="MNSGKFERWVEAYGRAWETKNPEAAADLFVADATYQETPFDEPM
                     RGRAEITEYWSSATGSQDEISFSYEILAASAGEGIAHWGANFVRLAKGTPVKIDGVLL
                     AKLDADGRCTEFREWWHSQ"
BASE COUNT          666 a         1400 c         1273 g          799 t
ORIGIN      
        1 tcgaagtcgt ccatcatctc aggacccatt acgccgtccc tgtagccagg gtagttctcc
       61 acgtcagagg tgagcatgag ctgcccgccg acctcgaaac cctggaacac cagcacgtcg
      121 aggttcgccc gagcggcgta cagggccgcc gtgtaccccg ccgggccgga gcccacgatc
      181 acaacgtcgt acgtatcgga catcaaacac tcctcttata cacttctata taaatctcta
      241 actttaccta ttctaataca tcgtgcaagc cggatggttc gtcctttcac acccggagcc
      301 ggcgaaacgc ggcggctcga actcgcacac acggccctgg aaagttatta ttggagccag
      361 ctaaccgcag cggacacagg aggtcggttt atggccgagc gagagaggga agcccacggc
      421 aagccggagc ccgaacgcta cttcgccgag tacgttccgc tgccgggcgg cgagggcgcc
      481 gcgctggtcg cgctgcaaga ggcgatcaac gagcacgccg cccacagctg gaagctgctt
      541 agcgtcgcag ccgacccgac gagtcagggc gtcttcatgg tctgggacac gaccggcttc
      601 ttctccggct aagctatcat tctgagatct cccctccgga gcacagcctt gctccgcggg
      661 gcggctctcc tcctcttcgc cgcgctcgcc gccgttctcg tgtggcgggc cggtgaagga
      721 cccgtcagtg cgctcgagac ccccgaagtg cgcccggcgc ccgggctctt cgatgagagg
      781 cgacgggagg cttccgaggt gctcgcgagc gtccgggtgg ggagcttcga gcggcccgaa
      841 gaggtgccgg gctacgagtt gctcgaggag atctcgaccg aggctgacgg cgcccggtcc
      901 gcgcagctaa tcgtggacac ccgctcgcgc ggccgggagg acttcaccct catcacccgc
      961 gacctcaagg cccgctacgc cggctacgac gtcgtctctg tagagttcac cgacacctcc
     1021 gaggtgctcg actaccacgg aggcgcgatc atcgtcaaca ccgctctcgg cgcccggaac
     1081 gcgggcttct actacgcgcc gcccagcgag ggctacctgg tgagggtcgt ggagtagtga
     1141 cgttgtatgc aacgtcattt ctcccggctg ctcactcgcg ccaactcgcc aggaacccct
     1201 cctgctccgg ggtcagggag tccacgccca tgcccacggc gtccagcttc atgcgggcga
     1261 cctcgtcgtc tatctcgttc gggaggaggt gaaccccgtt ctccatggtc cggctgttat
     1321 tcgcgagatg gtgagcgcag agcgcctgga ccgcgaaggt caggtccatg atctccacgg
     1381 gatgcccgtc gccagccgta aggttcacga ccatgccgcg cccgatcagg tgcagcttcc
     1441 ggccgtccgc catcgcgaac tcctcgacgt tgcgacgcac ctcgcggaca tctgaggaag
     1501 ctcttctcaa accttccacg tctatctcca cgtccacccc tccggcgtta gccaggatcg
     1561 cgccgtcccg catccgctcg aagtgcgcgg cggtgagcac gtcagtgctc cctgtcgccg
     1621 ttatgaacac ttcgcctatc tcggcagcgt cgagagccgg cagcacgtcg aacccgtcgg
     1681 cgtaggcctc caggccgtgc acagggtcaa cctcggagac tgtgactctg gctccgaggc
     1741 ctgcggcgta gcgggcgatg cccttgccgc accagccgta gccgagaacc acgacgcgtt
     1801 ttccgccgac catcaggttc gtgttctgca ttatggccgt gatcgtggac tgccctgtgc
     1861 cgtagcggtt gtcgaagcgg tacttgcacc gcgcgtcgtt tgcggcgatg acggggaact
     1921 cgaggatccc ctcgttgtcc atcgccctga acttcgcgac tccactcgtc gtctcctccg
     1981 acgccccgcg tatgtttccc agcagggtgc gcctcgtggt gtgggctagc cttaccagcc
     2041 ccgccctgtc gtctatgagg accgcctcgc ggtcgcccgc cgccgtatcg agcacccgct
     2101 ccagttcccg gtccgcctcc tccggtgggc tcgccgatga ggcgtagacc gtgacgccac
     2161 actcggcaag agcggcggcc acgtcgtcct ggacgaagaa aggaccgggg ctcgcgactg
     2221 cgacctcggc tccggcctcg gcgagcacga ccgtcaggta ggcggttttc gcttctatgg
     2281 ggagagcgac ccccacgccg aggccttcga aggtcccgtc tttgaggtac gtatcgcgca
     2341 ctctgttcag gaccggtgag tggcgtgcgg cccattcgat cttcctgtgc ccgtccggcg
     2401 cgaggccggg gtcccggacc agcgacttac tttgcatctc gctccctcct acctcgcggg
     2461 ggtgtccgtc gcgagcactt cctaggcggc caccacctgc tcccgtccgc ctagcatgaa
     2521 gctgacgacg atcatgagga aacgcaccgt cgaagactgg gacgacgtat catcagccta
     2581 ccgaagctga aggataacaa accgtccagt gcgctcacct caaggacttg aacgaccttg
     2641 agcacctgga atgaaccgtc tccggatcgc gcttccctct acctggcggt tttcaagccc
     2701 aacgccggta tgatctcctc cccgtatgcc tccagcgtct cctccatcgc gtcgtgcatg
     2761 aggtagatgt tgaactggtc cacgccgagg tctttgagtt cgtttagctt cgctacgtgg
     2821 tcctcgacgg tgccgagcac gcaaaagcgg tccacgatgt tgtccgggac gaagttcgtc
     2881 gagaggttgt ccgccttgcc gtggtggccg tagtcgtagc cctcgcgggc tcttatgtag
     2941 tcggtaagag cgtgcgggat ctcgccgtcc tcgccgtagc gggagacgag gtcggcgacg
     3001 tggttgccga ccatcccgcc gaaccaccgg cactggtcgc gctggtgggc caggtcgtcg
     3061 cccacgtagg cgggagcggc gacgcagacc tttacctcat cggggtcgcg gcccgcttcg
     3121 gtggcggcgt cgtggacgtg gcgcatggtc cactcgagga tcacggggtc cgctagctgc
     3181 aggatgaagc cgtcggcctt gcgccctatg gtgttgagcg ccttcggacc gtaacctgcc
     3241 atccagacgt ccagcttgcc gttactcacc catgggatgc ggaccttggt accgtcgtag
     3301 tctatctctc tgccttcggc cagctctttg atgacgtgca tcgcttcttc gacctcggcg
     3361 agcctggtcg gttttcttcc caggaccctc tgtgccgagt cgccgcgccc gatgccgcag
     3421 atagtgcggt tcccgaacat gtcgttgagg gtggcgaaag tcgacgccgt tacggagggg
     3481 tcgcgggtgg cggggttggt gaccaaggga cccacgatca tactctctgt cgcggagagg
     3541 atctgcgagt agatgacgta gggctcctgc cagaggatgt gcgagtcgaa ggtccaggcg
     3601 taggtgaagc caagagcctc ggccttgcgg gccagctcga cgacgcgcca ggctggcggg
     3661 tcggtctgca gggttacgcc gaagtccata ctcgctcctt ttctactact gcgagtgcca
     3721 ccactcgcgg aactcggtgc agcgtccatc cgcgtccagc ttcgcgagca agacgccgtc
     3781 gatcttgaca ggggttccct ttgccagcct cacgaaattc gcgccccagt gggcgattcc
     3841 ttctcccgcc gacgccgcga ggatctcgta ggagaagctg atctcgtcct gtgagcctgt
     3901 ggcgctggac cagtactccg tgatctccgc gcggcctctc atcggttcgt cgaatggcgt
     3961 ctcctggtag gtcgcgtctg cgacgaacag gtccgccgcc gcctcggggt tcttcgtctc
     4021 ccaggcccgg ccataggctt cgacccagcg ctcgaacttc cccgagttca tgtcgcctcc
     4081 ctcagttctt ccccgagacc gcagtggccg cgccgagggc tatgtagacc ccgccggg
//
LOCUS       DASTUV010000119         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_773798, whole genome shotgun sequence.
ACCESSION   DASTUV010000119 DASTUV010000000
VERSION     DASTUV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773798"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..763
                     /locus_tag="VFT03_02455"
     CDS             56..763
                     /locus_tag="VFT03_02455"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847034.1"
                     /translation="MTKRGETRVAVDLVVFGLLEGELSVLMGRRPHAPYEGFWALPGE
                     FRRPGQPVDELAREVLERTTGVERAWVEQLATFDRPPVFGESGDVSTPGRDPRGEILS
                     VAYMAVVGADRVRKAYGGDLGWQAVRRLPGQVAFDHREILDYAVRRLQSKLGYSSLGF
                     ELLAEEFTLPELQHVYEAVLGVALNRGNFRRKVLEAGVVEDTGKTRPARGKPAHLYRF
                     RRRAFGLFETPDGFIEG"
     gene            775..1689
                     /locus_tag="VFT03_02460"
     CDS             775..1689
                     /locus_tag="VFT03_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4847035.1"
                     /translation="MFGIGYFKAQPTEFVQKVAGGRIKREGQALSFYYLKRRTSVVVV
                     PVSSADAAFVFNEQTGNYQAVTIQGQLTYRIIEPRRTVEMLNYVIDPQTRMHVSQDPE
                     RLKQRIIVTVQMETRRQVEGRPLEGVLGNAASISAEVQEEVRSRDLLAPLGVELLSLN
                     FTSVSPNPEVGKALEAEYRESLLRRADEAIYARRAAAVEEEGKIKSNELEGEISLARE
                     RERLIDLEAANDRKKSQAWGERRELEAGFTARASEMELAPYRDFDPRKLLALAMRDIG
                     NNAGKVGNLNITSDLLADLLQTRTNADN"
     gene            1676..2626
                     /locus_tag="VFT03_02465"
     CDS             1676..2626
                     /locus_tag="VFT03_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847036.1"
                     /translation="MLTTEWDKIVVVTRKTGLEELLERFVTREQARFYLEHSGVPFEP
                     YEEEQRTHEEALTRLKAALPGGVRSQFVERSFLPTFAFGERDLVVTLGPDGMVVNVAK
                     YLRGAQLILAFNPDPSRIDGILIPFRVGEAMGTIQSVLHGDYRVREVTMARATLGDGQ
                     SLLAFNDLFIGRKTHVSARYRITHRRRSEDQSSSGIIVSTGAGSTGWLSSVLNGAAGV
                     VEPFAGAKAVAPVRSGFRFAWDAPRLCFSVREPFVSKASAAGIVWGNVRAGEKLEVVS
                     QMPQNGVIFSDGVEADYLAFDSGMVVSISVAEERARLVAA"
     gene            complement(2924..3352)
                     /locus_tag="VFT03_02470"
     CDS             complement(2924..3352)
                     /locus_tag="VFT03_02470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU4847037.1"
                     /translation="MTTARAHHEDTSSNPAFTALGDEEFLSLTTFRRSGQRVSTPMWV
                     TRDGDALVMFTPQASGKVKRLRNDPRVELRPCGRLGRVEEGVEPVAGVAEVLTDEASL
                     ERATGLLRRKYGLAYLVIISIERLIKFGPEKRLTLRITRT"
BASE COUNT          665 a         1058 c         1217 g          654 t
ORIGIN      
        1 tcaagtcgtg tcgcgcttgc tttatgcttc aatttatgat tatatataaa tcataatgac
       61 gaagagaggc gagacaaggg ttgctgtgga cctggtggtg ttcgggctgc tagagggtga
      121 gctctcggtg ttgatgggcc ggaggccgca cgcgccgtac gaagggttct gggcgttgcc
      181 cggggagttc cggaggccgg ggcaaccagt ggacgagcta gcacgcgagg tgctggagcg
      241 aacgactggt gtggagcggg cgtgggtgga gcagctcgcg acgttcgaca ggccgcccgt
      301 gttcggtgag agcggagatg tatcgacgcc ggggcgggac ccgcgggggg agatcctatc
      361 ggtggcctac atggcggttg tcggagcgga ccgagtgcgg aaggcgtatg gcggggatct
      421 tggatggcag gccgtccggc ggctgccggg gcaggtggcc ttcgaccacc gtgagatcct
      481 ggactacgcc gtgcgacggt tgcagagcaa gctcgggtac tcgtcgctgg ggttcgagct
      541 cctggccgag gagttcacgc tgccagagtt gcagcacgtc tacgaggcgg tgctcggggt
      601 cgcgttgaac cggggaaact tccggcgcaa ggtgcttgaa gcgggggtgg tggaggatac
      661 ggggaagacg aggccggcgc gcggtaagcc agcgcacctg tacaggtttc ggcggcgggc
      721 cttcgggctc ttcgagacgc ccgacgggtt tatcgaggga taaggaggta gaggatgttc
      781 gggatcgggt acttcaaagc gcaaccgacg gagttcgtac agaaggtggc cggaggacgg
      841 ataaagagag agggacaggc cctctctttc tactacctca agcgccgaac gagtgtcgtg
      901 gtggtgcccg tctccagcgc tgacgcggcg ttcgttttca acgagcagac cggaaactac
      961 caggcagtca cgatccaagg acagctcaca tatcgcatca tcgagccccg ccggacggtg
     1021 gagatgctca attacgtgat cgacccgcag acgcggatgc acgtctccca ggacccggaa
     1081 cggctgaagc agaggatcat cgtcaccgtc cagatggaga ctcggcggca ggtggagggg
     1141 agaccgcttg aaggggtgct cgggaacgcc gcgagcatct cggcggaggt ccaggaggaa
     1201 gtgcgctccc gcgacctgct ggcgccgctc ggggtggagc tgctgagcct gaacttcacc
     1261 tccgtaagcc ccaaccccga ggtcggaaag gcgctggagg cggagtacag ggagtcgctg
     1321 ctacgacgag cggacgaggc aatctacgca cggcgggcgg cggccgtcga ggaggaaggc
     1381 aagatcaagt cgaacgagct agaaggtgag atctcccttg cccgggaacg cgagcgcctt
     1441 atagacctcg aggccgccaa cgaccgcaag aagtcccagg cgtggggcga gcggagggag
     1501 ctggaggctg gcttcacggc gcgggccagt gagatggaac ttgctccgta ccgcgacttc
     1561 gatccccgga aactcctcgc tctcgccatg cgtgacatcg gcaacaacgc gggcaaggtc
     1621 ggcaacctca acatcacctc cgatttactt gcggaccttc ttcagacaag gacgaatgct
     1681 gacaactgag tgggacaaga tcgtggtcgt caccaggaag acaggcctcg aagagcttct
     1741 cgaacgcttc gtgacgcggg agcaggcccg tttctacctg gagcactccg gcgtaccctt
     1801 tgagccctac gaggaggagc aacgcaccca cgaggaagcc ttgactaggc tcaaggctgc
     1861 cctccccggc ggcgtccgca gccagttcgt cgagcgctcg ttcctcccca cgtttgcatt
     1921 tggggagcgg gatctcgtcg tgaccctcgg gcccgacggc atggtagtca acgtcgcaaa
     1981 gtacctgcgc ggcgcgcagc tgatcctggc cttcaacccc gacccctcga gaatagacgg
     2041 catcctaatc cctttccggg tgggtgaggc tatgggaacg atccaatccg tccttcacgg
     2101 tgattaccgc gtgcgcgagg tgacaatggc tcgcgccacg ctcggcgacg gccagagtct
     2161 tctagccttc aacgacctct tcatcgggag gaagacccac gtttcggccc gctaccgcat
     2221 tacccacagg cgacgctccg aggaccagtc ttccagcggc atcatagtct cgaccggggc
     2281 ggggtccacc ggctggctca gctccgtcct caacggcgcg gcgggcgtcg tcgagccgtt
     2341 tgccggggcg aaggccgtgg cgccggtccg cagtggattc cgttttgcct gggacgcgcc
     2401 gaggctgtgc ttcagcgtcc gtgagccgtt cgtcagcaaa gcatcggcgg cggggatagt
     2461 gtggggcaac gtccgcgcgg gcgagaagtt agaggttgtc agccagatgc cgcagaatgg
     2521 tgtgatcttc agcgacggcg tcgaggccga ctatctggcc ttcgactccg gtatggtcgt
     2581 cagcatctcc gtcgccgaag agagggcgag gctcgtcgct gcgtaaagtt gtttgtattt
     2641 ctggacgtat atgccgcgtg caactcccag agtacataac tgcctgctcc cacatgtcac
     2701 ttcgtcgtgg gtgacgagag ccgagaaatt cgcttcaagc ggctgctcgt agataggact
     2761 actaggatgg agtggacagg tcacgggttt ccgcgtgcag gcgcgaactt caccgtgttg
     2821 cgcagaagta ggcgttctca tagaagtcag tatgcaggat cctgcatggc cccggtccta
     2881 ttcatcacgc atccctcgcg cgctgacgcg gcgacgccgc tcctcaggtg cgggtgatgc
     2941 gcagggtcaa ccgcttctcc gggccgaact tgatcagccg ctcgatgctt atgatcacca
     3001 ggtacgcgag cccgtacttc ctgcggagta gcccggtcgc ccgctccagg ctcgcttcat
     3061 ccgtcagcac ctccgccacg ccggcgacgg gttccacgcc ttcttctacg cgcccgagcc
     3121 ggccgcaagg acgcagctcg acgcgtgggt cgttgcgcag ccgcttcacc ttgccgctcg
     3181 cttgcggggt gaacatgacc agtgcatcgc cgtccctcgt gacccacatc ggggtcgaga
     3241 cgcgctggcc ggacctgcga aaggtcgtga gggagaggaa ctcctcgtcg cccagggcgg
     3301 tgaaggcggg gttgctggag gtgtcctcgt ggtgggctcg ggcggtagtc atggtaggag
     3361 tcctctctcc gaggggcgta tcaaggcatg ccggtcacgg cagcacccac taatatatac
     3421 gctcctggca cgcacccgcc cgtgagcccg attactgggc tattattcac ccacctttcg
     3481 agagtagagt tctcagaagg ccgcatagcc ccggttcgat gccacatacc cctctaggat
     3541 cggcgcgctc ttgtgcacgc ataagccgcc cataataccc tgtgctggtc ctag
//
LOCUS       DASTUV010000120         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1209159, whole genome shotgun sequence.
ACCESSION   DASTUV010000120 DASTUV010000000
VERSION     DASTUV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209159"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1428
                     /locus_tag="VFT03_02475"
     CDS             184..1428
                     /locus_tag="VFT03_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU4847038.1"
                     /translation="MWKSTATGRDRRRTVTWVLLVVVLLLATALAAYAYGRSQSPAGL
                     GERDRESLALYAQALDTVRDDYVDQEAIDSKKQTYGAIEGMLDTLGDDGHTRFLTPEE
                     RVENREGLSGTYVGIGIQLEEKDGEVVVASPIEGSPADRAGIEAGDVVVAVDGKSVRE
                     EGVTQVVEKIKGPEGTQVKVTVSRDGEERVFDLERAEIESPVASWTMIPSSDVAFVRL
                     SSFSDDSAEKLAQAFEEARSAGARRFILDLRNNPGGRLDQAVRMAGDFLEPGSVVYIR
                     EDASGEREEIEADGGAERTRAPLAVLVNGGTASSSEILAGALRDNNRAPVIGETTFGT
                     GTVLSEFVLRDGSAILLGVAEWLTPDGDFIRESGIQPDIRVPLASGDDPLTPTDARDL
                     SRDEILERDAQLARAFENLQRQ"
     gene            1505..1972
                     /locus_tag="VFT03_02480"
     CDS             1505..1972
                     /locus_tag="VFT03_02480"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4847039.1"
                     /translation="MAQRVHESIEVQAPLQDVFRYWSNFENFPSFMQNVEEVRMSGRD
                     TSHWRVKGPLGKSVEFDARTTEMDPDRGIGWNTTGGEVMTSGEARFEEVSPGRTRIEV
                     TMNYADPPGGKVGEVAANLISNPERNLREDVENFARIVERGELGGPGAQTPSR"
BASE COUNT          387 a          608 c          714 g          307 t
ORIGIN      
        1 gtcagcattt cagcttctct ctgcgctgag agaagctgac aagccgcctg gcgaagccag
       61 gtggcgtgct gacaagcgag gcccggaggg ccgaagcgtg ctgacaagcg gaagccgaag
      121 gctggagcgt gcggacaagc gaagcccgaa gggcgtagcg tgctgaaata gtacaatcct
      181 tgcgtgtgga agagcactgc aaccgggcgt gaccgtcgcc gcaccgtaac gtgggtactc
      241 ctcgtcgtgg tgttgctgct ggcgacagcg ttggcggctt atgcctacgg gcgatcgcag
      301 agcccggcgg ggctggggga gcgggaccgc gagagcctgg cgctctatgc acaggcgctc
      361 gacacggtcc gcgacgacta cgtcgatcag gaggccatcg actccaaaaa gcagacatac
      421 ggcgccatag agggcatgct ggacactctg ggggatgacg ggcacacgcg ctttctcacc
      481 cccgaggagc gcgtggagaa ccgcgagggt ctctccggga cgtacgtagg gatcgggatc
      541 cagctcgaag agaaagatgg cgaggttgtc gtcgcctccc cgatagaggg ctcgccggcg
      601 gacagggcgg ggatcgaagc cggcgacgtc gttgtcgccg tggacggtaa gagcgtgcgc
      661 gaggagggcg tgacacaggt cgtcgagaag atcaagggac ccgagggcac gcaggtcaag
      721 gtgaccgtct cgcgcgacgg cgaggagcgc gtgttcgacc tcgagcgcgc cgagatcgag
      781 tcgcccgtgg cctcgtggac gatgattccc tcaagcgacg ttgcgttcgt gcgcctgtcc
      841 tccttctctg acgatagcgc ggagaagctt gcgcaggcct tcgaggaggc gcgctcggcc
      901 ggcgccaggc gctttatcct cgacctccgg aacaaccctg gcgggaggct cgaccaggct
      961 gtgcggatgg ccggggactt cctcgagccg ggcagcgtcg tatacatccg cgaagacgcc
     1021 tcgggcgagc gcgaagagat cgaggccgat ggcggcgcgg agcgcactcg cgccccgctg
     1081 gcggtcctgg tgaacggcgg gaccgcgtcg agctcggaga tcctggccgg cgctttgagg
     1141 gacaacaaca gggcgccagt gataggggag accactttcg gcacggggac ggtgctctcg
     1201 gagttcgtcc tgagggacgg ctcggcgatc ctgctcggcg tcgccgagtg gctcacgccg
     1261 gacggcgact ttatccgcga gtccggcatc cagccggaca tccgggtgcc gctagcgtcg
     1321 ggtgacgatc cgcttacccc caccgatgcc cgcgaccttt ccagggatga gatcctcgag
     1381 cgggacgccc agctcgcgcg cgccttcgaa aacctccagc gtcagtagca aggttcacat
     1441 aaatggtcgt tctgattagg catccttacg agtgacattt gacgcacgag cgaaaggaga
     1501 gcttatggct cagcgcgtac acgagagcat agaggtacag gccccgctgc aggacgtctt
     1561 tcggtactgg tccaacttcg agaacttccc gagcttcatg cagaacgtcg aagaggtccg
     1621 aatgagcggc cgggacacca gccactggag ggtcaagggt cctctgggca agagcgtcga
     1681 attcgacgcc aggactaccg agatggaccc ggaccgcggc atcggctgga acacgaccgg
     1741 aggcgaggtc atgacgagcg gcgaggcgcg cttcgaggag gtctcccctg gccgcacgcg
     1801 gatagaggtg acgatgaact acgccgaccc gccagggggc aaggttgggg aagtcgcagc
     1861 taacctcatc tccaaccccg agcggaacct cagggaggac gtcgagaact tcgccaggat
     1921 cgtcgagcgt ggcgagctcg gcggtcccgg cgcccagacg ccgagccgct agccgcgcgc
     1981 tcccgccaac cgattatcgg ttaccatgag gccgtc
//
LOCUS       DASTUV010000121         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1345036, whole genome shotgun sequence.
ACCESSION   DASTUV010000121 DASTUV010000000
VERSION     DASTUV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345036"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFT03_02485"
     CDS             complement(<1..365)
                     /locus_tag="VFT03_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU4847040.1"
                     /translation="MPLVDLDRFDFLYSERVKGMKTAATRDLMATLSRPGIISLAGGF
                     PDTRAFGEEAFREISGSIAQDTAQALQYGPTSGLEAIKDVIVDVMAAEGTQGRQEDVF
                     VTTGAQQGLDLIAKVFLDEG"
     gene            complement(366..953)
                     /locus_tag="VFT03_02490"
     CDS             complement(366..953)
                     /locus_tag="VFT03_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847041.1"
                     /translation="MQEVRVESLDGLEVRESELRLSANAGRLRIVFPREVWEHVLMSV
                     EASEEPDAAELEELYDLREKLKAHAAMQAFEIALEGVGAGEPGLDYDLVLRPGGLLAV
                     TTGYLFVPVVERELMRRIGACLVGDRLVEQAYAIGAVASEQALAAGDDNLKALSVRGR
                     RCSTSRFEMAPELWQVLAEGLGWDNVETFVNDGGV"
     gene            1002..1574
                     /gene="rdgB"
                     /locus_tag="VFT03_02495"
     CDS             1002..1574
                     /gene="rdgB"
                     /locus_tag="VFT03_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU4847042.1"
                     /translation="MRAVFVTTNEDKRREAQRILGVELVDAALDPQDVPEIQALDFAE
                     VAVHKARAAREALGFPPYAVLVEDSGLVIGAWNGLPGALTKWFLASVGNEGILTMLSG
                     EDRSARAVCAVAVADAGGELRVFRGEVEGEISPEPRGDGGFGWDPIFVPKGSHLTYAE
                     MGENKHQDSHRARAFRQVRGWLEEQDRREV"
     gene            1690..2751
                     /locus_tag="VFT03_02500"
     CDS             1690..2751
                     /locus_tag="VFT03_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4847043.1"
                     /translation="MSAENGHPEKGEEQARNEGAVATDTDRLVNLYHEMVLIRAFEEA
                     CGRGFRQGKIGGYLHVYIGQEAVATGFLEAFKEGDKVITAYRDHAHALLLGSDPKNVM
                     AELYGKGAGIVKGKGGSMHLFDVERGLMGGYGIVGGHIPPGVGLAYAMRYHETDNITQ
                     LYLGDGAMSTGAFHEAANLSGLWGKDGMNPCLFIVENNQYGMGTSVERTTAMTDLAAK
                     FDAYGIENEKVDGMDLETVIEVAERTTEQVRESGKPYAVEELTYRTAPHGAADFFEKY
                     RTKEEIEEWRQRDPIGLLEKKLLERGALDDEKLEEIKAEAKETVQEAIKFAEESEEPP
                     IEELYTDVYAGEDEYLGEK"
     gene            2751..3743
                     /locus_tag="VFT03_02505"
     CDS             2751..3743
                     /locus_tag="VFT03_02505"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU4847044.1"
                     /translation="MAETKTYREALREAMVMEMDRDENVVLMGEDIGVYGGTHLITDG
                     LIDQYGPKRVIDTPISELGFTGAAVGMAFQGMRPIVEMMTWNFSFLAADQIIQNAAKV
                     RYFSGGQVEVPMVIRGPNGGGVQLSAQHTHSLENLYGHFPGLKVVSPVTPNDVKGMML
                     TAVRDPNPVIFLEAGALYGTKGEVEEGDNAIPFGKARIAREGSDVTLVAYGRQVNLCL
                     RAADTLEEEDGVSAEVIDLRSIRPFDEDAVVESVEKTHRAVAVQEQWRWFGVASEVAA
                     IIQDRAFDYLDAPVERVSGAEVPAPYARNLELAAFPSEKQVANAARRVLYMEEK"
     gene            3754..>4246
                     /locus_tag="VFT03_02510"
     CDS             3754..>4246
                     /locus_tag="VFT03_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU4847045.1"
                     /translation="MIMPKMGDAMEEGTLVKWLKSEGDEVSEGDPIAEIETDKVTLEL
                     EAEDSGTLAQLIADEGQDIPVGEAIAFIAGEGEEVPERAAGGGAEEAEEEGGEGEAQA
                     QTATETEAPEEEGEEEAPAQADGRTDGHFRASPIVRRLAQENNLDLSRIEGSGPAGRI
                     VERD"
BASE COUNT          847 a         1274 c         1478 g          647 t
ORIGIN      
        1 ccctcgtcga ggaagacctt ggcgatgagg tcgagcccct gctgggcgcc ggtggtgacg
       61 aagacgtcct cctgcctgcc ctgggtgccc tccgcggcca tcacgtccac tatgacgtcc
      121 tttatggcct cgagacccga tgtaggaccg tactgcagcg cctgggcggt atcctgggcg
      181 atgctgcccg agatctcccg gaacgcctcc tcaccgaagg cccgggtgtc gggaaagccg
      241 ccggctagcg agatgatgcc tggccgggac aacgtagcca tgaggtcgcg ggtggcagcg
      301 gtcttcatgc ctttgacccg ctccgagtag aggaagtcga agcggtcgag gtctacgagc
      361 ggcacctaca cacccccatc gtttacgaag gtctcgacgt tatcccatcc cagcccctct
      421 gcgagtacct gccagagctc cggcgccatc tcgaagcggc tcgtgctgca gcgtctgccc
      481 cggacggaga gggccttgag gttgtcgtcg ccggcggcca gggcctgctc gctggctacg
      541 gccccgatcg cgtacgcctg ctcgacgagg cggtcgccca cgaggcaggc cccgatccgg
      601 cgcatcagct cacgctcgac cacggggacg aagaggtagc ctgtcgtcac ggcgaggagc
      661 cctccgggcc ggagcacgag atcgtaatcg agccccggct cgccggcccc cacaccttcg
      721 agagcgatct cgaaggcctg cattgcggcg tgggctttga gcttctctct gaggtcgtac
      781 agctcctcga gctccgctgc gtccggctct tctgaggctt cgacgctcat caggacgtgt
      841 tcccagacct ccctggggaa tacgatgcgt aagcgcccgg cgttggccga gagcctgagc
      901 tcgctctccc gcacctccag cccatccagg ctctccaccc gaacctcttg catgggagcg
      961 actgtatcat aaagagcaca tgggttgtag acttccgcgc catgagagcc gtcttcgtga
     1021 ccaccaacga ggacaagcgc cgcgaggcgc agaggatact tggggtcgag ctcgtagacg
     1081 ccgccctgga tccccaggat gtccccgaga tacaggccct cgacttcgca gaggtcgccg
     1141 tccacaaagc gcgggctgcc cgtgaggccc ttggcttccc gccttatgcg gtcctcgtcg
     1201 aggactctgg gctcgtcatc ggggcctgga acggcctgcc gggtgcgctg accaagtggt
     1261 tcctcgcgag cgtcggcaac gagggcatcc tgacgatgct ctccggcgag gaccgctcgg
     1321 cgcgcgccgt ctgcgcggtc gcggtcgcgg acgcaggggg ggagttgcga gtcttcaggg
     1381 gagaggtcga gggggagatc tcgcccgagc cgagggggga tggtgggttc gggtgggacc
     1441 cgatcttcgt cccgaaaggg agccatctca cgtacgccga gatgggtgag aacaagcatc
     1501 aagactcgca cagggcgagg gcattccggc aggtccgcgg gtggttggag gagcaggata
     1561 gacgagaagt ctgacgagcg cgcatacatc tgggcgaaac gggcaataaa atacacgtac
     1621 gagatggcga agagtgctat tctgttcgtc gttcgttggg ttgtagggcg aggtgggaag
     1681 gggccgtcta tgtcggcaga gaacgggcac ccggaaaaag gggaagaaca agcccgtaac
     1741 gagggcgcgg tggccacaga cacggatcgt ctcgtgaacc tctaccacga gatggtgctc
     1801 atccgggcgt tcgaggaggc gtgcgggagg ggctttaggc agggcaagat cgggggctac
     1861 ctccacgtct acatcggcca ggaggctgtg gcgaccgggt tcctcgaggc cttcaaggaa
     1921 ggcgacaagg tgataaccgc ctaccgggac cacgcgcacg cgctcctgct gggtagtgat
     1981 cccaagaacg tcatggccga gctctatggt aagggagcgg gcatcgtcaa gggcaagggt
     2041 ggctcgatgc acctcttcga cgtcgagagg ggcctcatgg gcggctacgg gatcgtcggc
     2101 ggccacatac ctcctggggt cggcctcgcc tacgcgatga ggtaccacga gacggacaac
     2161 atcacgcagc tctacctcgg ggacggcgcg atgagcacgg gcgccttcca cgaggccgcc
     2221 aatctctcgg ggctatgggg caaggacggc atgaacccgt gcctcttcat agtggagaac
     2281 aaccagtacg ggatgggcac gagcgtggag cgcactaccg cgatgacgga cctcgccgcc
     2341 aagttcgacg cctacggtat agagaacgag aaggtggacg ggatggacct cgaaacggtc
     2401 atcgaggtgg ctgagaggac gaccgagcag gtaagggaga gcggcaagcc gtacgccgtc
     2461 gaggagctga cctacaggac tgctccccac ggtgccgcgg acttcttcga gaagtaccgt
     2521 accaaagaag agatcgaaga gtggcgccag cgcgacccga ttgggctgct ggagaagaag
     2581 ctcctggaga gaggagcatt agacgacgag aagctcgagg agataaaagc cgaggccaag
     2641 gagacggtac aggaggcgat caagttcgcc gaggagagtg aggagccccc gatcgaagag
     2701 ctctacaccg acgtctacgc tggtgaagac gagtacctgg gggagaagta atggccgaga
     2761 ccaagaccta ccgcgaggcc ctgcgcgagg ccatggtcat ggagatggac cgcgacgaga
     2821 acgtcgtctt gatgggcgag gacatcggcg tctacggcgg gacccacctc atcaccgacg
     2881 ggctcatcga ccagtacggt cctaaaagag tcatagatac ccccatctcc gagctcggct
     2941 tcaccggggc ggcagtcggc atggcgttcc agggcatgag acccatagtg gagatgatga
     3001 cctggaactt ctcgttcctc gctgcggacc agatcatcca gaacgccgcc aaggtgcgct
     3061 acttctcagg gggccaggtg gaggtgccca tggtgatccg cggccccaac ggcggcggcg
     3121 tccagctcag tgcgcagcac acccacagcc tcgagaacct ctacggtcac ttccccggcc
     3181 tcaaagtcgt ctcccccgtt acgccaaacg acgtgaaggg aatgatgctc acggccgtga
     3241 gggatcccaa cccggtcatc ttcctcgagg caggcgccct ctacggcacc aagggcgagg
     3301 tagaggaggg agacaacgcc atccccttcg gaaaggcgag gatagcgcgc gagggctcgg
     3361 acgttacgct cgtcgcctac gggcggcagg tgaacctgtg cctgagggcc gccgacacgc
     3421 tcgaagagga agacggcgtc agcgccgagg tcatagacct caggtccatc cggcctttcg
     3481 acgaggacgc cgtcgtcgag tcggtcgaga agacgcaccg ggccgtggcg gtgcaggagc
     3541 agtggcggtg gttcggggta gcgagcgagg tggctgcgat catccaggac agggcgttcg
     3601 actacctgga cgccccggtt gagcgggtta gcggagcgga ggttcctgcg ccatacgcgc
     3661 ggaaccttga gctcgcggcc ttcccgagcg agaagcaggt ggctaacgcg gcacgcaggg
     3721 tgctctacat ggaggagaag taagttgccg gaagtgatca tgcccaagat gggcgatgcg
     3781 atggaggagg gcaccctcgt caagtggctc aagagcgagg gtgacgaggt ttccgagggc
     3841 gacccgatag ccgagatcga gaccgacaag gtgacgctgg agctcgaagc cgaagactcc
     3901 ggcacgctcg cccagctcat cgccgacgag ggccaggata tcccagtggg cgaggcgata
     3961 gcctttatcg ccggcgaggg cgaggaggtg ccggagcggg ccgctggcgg cggagccgaa
     4021 gaagccgagg aagagggcgg cgaaggcgaa gcccaggccc agaccgccac ggagaccgag
     4081 gcgcccgagg aagaaggtga agaggaggct cccgctcagg ccgacggccg tacggacggc
     4141 cacttccgag cctctccgat agtgaggcgc ctggcccagg agaacaacct ggacctctcc
     4201 aggatagagg gttcgggacc ggccggtcgc atcgtcgagc gcgacg
//
LOCUS       DASTUV010000122         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_631134, whole genome shotgun sequence.
ACCESSION   DASTUV010000122 DASTUV010000000
VERSION     DASTUV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631134"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFT03_02515"
     CDS             complement(<1..531)
                     /locus_tag="VFT03_02515"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEU4847046.1"
                     /translation="MERTIGWTEALVTAKVAVVGAGGQAQSRSLYNACVTQRPITTTE
                     DQSATFIELFFDLVFVFAVTQTVSLIHEDLSWAGVGHAVVVFWLVWWAWTQYTWALNS
                     ADTTHPGVDAVMLAATAIAFLMAVTIPDAFGDAGGFFAATYLAVRLVGLGLYGRVAWD
                     DVAKRSAVRRFAVLSLG"
     gene            673..1281
                     /locus_tag="VFT03_02520"
     CDS             673..1281
                     /locus_tag="VFT03_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU4847047.1"
                     /translation="MGERKTVRFYYSVGSRYSYLASTQVGKLERETACQVLWRPLYSV
                     DLYELRGISPFEVGPVSGQYDWDYRRYDAECWAEYYGVPYKEPRGAVEFDARLLARAC
                     AAADALGAVRPYSESMFKAIFAEGLPEIGEGECLERAEAVGLSREAFARELASPGPER
                     RLAETAAEAHSAGAFGVPTFVVDDRMFWGNDRLVLVRHFLKK"
     gene            1630..1962
                     /locus_tag="VFT03_02525"
     CDS             1630..1962
                     /locus_tag="VFT03_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847048.1"
                     /translation="MGAEARRSVRELPIACGLSDLEQRKRREELSRQLFSGCQRTDEL
                     DDGYEFVFPGGPEWAEKLVTFVASERECCPFFGFEMIFESGQGPISLRVRGPEGTKEF
                     VREEFVCG"
BASE COUNT          361 a          631 c          673 g          365 t
ORIGIN      
        1 gccgagggac aggacggcga accggcgcac ggcactccgc ttggccacat cgtcccaggc
       61 cacccggccg tagaggccaa gaccgaccag gcgcaccgct aaataggtcg cggcgaagaa
      121 gccgccagcg tctccgaaag catccgggat ggtcacggcc ataagaaagg cgatggccgt
      181 ggccgccagc atgacggcat ccacgccagg atgggtcgta tcggcggagt tcagcgccca
      241 ggtgtactgc gtccaagccc accacaccag ccagaacacg accaccgcat gcccgacccc
      301 ggcccaggac agatcctcgt gtatcaggct gacggtctgg gtcacagcga agacgaacac
      361 cagatcgaag aagagctcga tgaacgtcgc gctctggtcc tcagtggtgg tgatcggccg
      421 ctgcgtcaca caggcattat aaagcgagcg gctctgcgcc tggccccccg cgcccacaac
      481 cgctaccttc gccgtcacga gcgcctccgt ccatccgatg gttcgctcca cctcttctca
      541 tgtcacgagc ctggcagaaa caagcggatc ggcgggttta agcatagtcg cacggcgtgc
      601 ctccctcttc cgcctggttg ccggatgtgg catcctcttt ttcgttcagg agtacgtccc
      661 aacgggttaa acatgggtga gcggaagacg gtgcggttct actactcagt cggcagccgc
      721 tactcctacc tggcctccac ccaggtgggt aagctcgaac gcgagacggc ttgccaggtc
      781 ctctggcgcc cgctttacag cgtcgacctc tacgagttgc gcggcataag ccccttcgag
      841 gtagggcccg tctcgggcca gtacgattgg gactaccgtc gctacgacgc cgaatgctgg
      901 gccgagtact acggcgttcc ctacaaggag cccaggggtg ccgtggagtt cgatgcccgg
      961 ctgctcgctc gtgcttgcgc cgcggcggac gcgctgggcg ccgtgcgccc ctacagcgag
     1021 agcatgttca aagctatctt cgcggagggc ttacccgaga tcggcgaggg agagtgcttg
     1081 gaacgggccg aagcggtcgg cctgtccagg gaggccttcg cacgcgagct tgcctcgccg
     1141 gggccggaac ggcgactcgc cgagaccgcc gcggaggcgc actccgccgg ggcgtttggg
     1201 gtacccacct tcgtcgtcga cgacaggatg ttctggggta acgaccggtt ggtgttggtg
     1261 cgccacttcc taaagaagtg acgccgtagc gtccccgcag cccacagaga aacgagccct
     1321 tcttgaggat tctcggggta gaccgccgag cgcgtgggcg gagatgtcgg gcttcttctg
     1381 ctgccgtctc gttcgaccag gttccgtagc acgtatcgct ggtccggcga gagcacttcg
     1441 ggagcgagct gctgctgcag cggtacctcg acgcctctgt cgggattgct cggtgaaccc
     1501 ggccgggccg cctccccgac ggacggctcg gaggctaaac tctttcccct tgacttgaag
     1561 tgcactttaa gtgttacgct cgcagatagg atggcacgag cacccgtcca ccaacagagg
     1621 aggtcgaaca tgggtgccga agcaagacgt tccgtgaggg agttgccgat agcctgcggc
     1681 ctgagcgatc tggagcagag gaagcgccgg gaggagctct cgcgccagct cttctccggt
     1741 tgtcagagga ctgatgaact cgacgatggc tacgagttcg tgttcccggg agggccggag
     1801 tgggccgaga agctggtgac gttcgtggcc tctgagaggg agtgctgccc cttcttcggc
     1861 ttcgagatga tcttcgagtc ggggcaagga cctatctcgt tgcgggtgcg tggccccgag
     1921 ggaaccaagg aattcgtgag agaggagttc gtctgtggct agtattgcgg tgagcggttc
     1981 tcgccgacgg gctcgcggtg ccgggtcgaa ttgacctggt cctcgggagc
//
LOCUS       DASTUV010000123         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_98672, whole genome shotgun sequence.
ACCESSION   DASTUV010000123 DASTUV010000000
VERSION     DASTUV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98672"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFT03_02530"
     CDS             <1..595
                     /locus_tag="VFT03_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4847049.1"
                     /translation="LLSIVLAFFFVASFIFVGLGTNVSYNLFDLIGGDDKQAGEQTTN
                     PQDQIQKAEKQLEEHPNDPDAIKELSALYYQSGQYEDAARVLQDGREVAPKDPEIPFL
                     LGQVYYKEAQATPGKEQDKLYQQAGDAFAAAAEIDPENEDAYLLAGDSYDRAGEPALA
                     IKYYNGYLDLEPEGKQSKAVKERISTLLEGGEETTSG"
     gene            672..935
                     /gene="tatA"
                     /locus_tag="VFT03_02535"
     CDS             672..935
                     /gene="tatA"
                     /locus_tag="VFT03_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4847050.1"
                     /translation="MPSIGGPELIIALVIILLLFGAKRIPELAKGLGTGMREFKKGSR
                     GEYEVKDEKEKDNNEELTAGEDKTETRAEDAPTENDRAEQKRG"
     gene            965..1882
                     /gene="tatC"
                     /locus_tag="VFT03_02540"
     CDS             965..1882
                     /gene="tatC"
                     /locus_tag="VFT03_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU4847051.1"
                     /translation="MAGRLRLPSRPRDEARMTLIEHLDELRSRIIKVGVAFVLAATVA
                     WFFREQIFTFLMEPAGDRLHGKLYVTSVAEQFINDLKLALYVAFVVTIPIFLYQAWAF
                     VAPAVGDVGRVFTYILITLASSLFVAGIAFGYFFVLPVGVQFLLGWDTSRYIPIITPT
                     NYLAFVTRFLLACGIVFELPAATYVGAKLELIDASLLKKYRRHAIVANTILAAALTPT
                     PDPFSMILLAIPMIFMYELSIVIARYVNPTPEVAVHELAANEDEDDDYDDYDEDDDYD
                     EDYEEVEPVYDGVERRDDDDEDDETERDL"
     gene            complement(2016..2930)
                     /locus_tag="VFT03_02545"
     CDS             complement(2016..2930)
                     /locus_tag="VFT03_02545"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEU4847052.1"
                     /translation="MNATNPRGSIFSEWFRLARPFSLTASAVPVLFGTALALKDGAFA
                     WGPFLAMLFASLLIQAATNMFNEFYDEQRGLDTATAVGIAGSIVRGRMRARAVLLGAL
                     FCYTVALVLGIYLVAVGGWQILALGCLCALGGYLYSAGPRPIAYTPVSEAAVFLFMGV
                     LIVVIAYAVQIPGSFPAYVPLAALPIGGPVAAILLANNIRDMRSDRRGGRRTLPIVLG
                     REVAILLYRVLLLEAYVAVAVLMALRIVPLECAVVFLSATPAIRLWRDIATTTTPERL
                     DPVVKRTAGLHLVFGLLYTAGVLLGWLL"
     gene            3157..4050
                     /locus_tag="VFT03_02550"
     CDS             3157..4050
                     /locus_tag="VFT03_02550"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEU4847053.1"
                     /translation="MEAGGTMGIAGTYLRLVRPKQWTKNGFVLAGVVFAHEALMVSSV
                     VKALLAFAAFCALSGAVYAANDVFDVEEDRKHPRKRSRPVASGEISPRSALVFAAVLA
                     AAGLGLGFAVDLGVGLAGIAYLALQALYTTTLKHMAILDVMCISAGFVIRALAGVAAV
                     SRPISPWLIVCTALLTLFLGFSKRRHELASLGEGATIHRRNLADYSVPLLDEMMNIMI
                     SATIIAYALYTFFEYDNYYMMASIPFVVYGVFRYMLLVHRNGGGNPDTLLLADRPLQI
                     ALLLWLVVAGVVIYVIPLWNG"
     gene            4038..>4364
                     /locus_tag="VFT03_02555"
     CDS             4038..>4364
                     /locus_tag="VFT03_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU4847054.1"
                     /translation="MERLKHNLILSLALGVAVYLVLAVVSGFGSLTAALEDFDLALVP
                     AILGLVLLSYAGRFLRWLYYLKLLKVSVPLPTNAAIFAAGLSMTISPGKLGEVLKSVF
                     IRQASGA"
BASE COUNT          831 a         1393 c         1348 g          792 t
ORIGIN      
        1 gctgctctcg atcgtgctcg ccttcttctt cgtagcctcc ttcatcttcg tgggcctcgg
       61 caccaacgtc agctataacc tgttcgacct gatcggaggc gatgacaagc aggccggaga
      121 gcagacgacc aatccccagg accagatcca gaaggccgaa aaacaactcg aagagcaccc
      181 gaatgaccct gacgccataa aggagctctc ggccctctac taccagtccg gccagtacga
      241 ggacgcggcc cgggtgctgc aagacggacg cgaggtcgct cccaaagacc ctgagatacc
      301 tttcctgctc gggcaggtct actacaagga ggcccaggcc acgccgggca aggagcagga
      361 caagctctac cagcaggccg gcgacgcgtt cgcggcggcc gccgagatcg atcccgagaa
      421 cgaggacgcc tacctgctcg cgggtgattc ctacgacagg gcgggcgagc cggccctggc
      481 catcaagtac tacaacggct acctggacct cgagccggaa ggcaagcagt cgaaggcggt
      541 caaggagcgc atctcgacgc tgctcgaggg cggcgaggag acgaccagcg ggtaggcgtg
      601 atccggtttt cgaaaccagg tcgtataatg tggcgtaata tcctggagac cgtcagttag
      661 gaggagagac aatgccgagc atcgggggac cggagttaat tattgccctc gtcatcatcc
      721 tgttgctctt cggtgcgaag cgcatcccgg aactcgccaa agggcttggg accggtatgc
      781 gcgagttcaa gaagggctcg cgtggcgagt acgaggtcaa ggacgagaaa gagaaagata
      841 ataacgaaga gctcaccgcc ggcgaggaca agaccgagac ccgcgccgag gacgctccca
      901 cggagaacga cagggccgag cagaagcgcg gctagagctc ctgacgaacc tctgaggaat
      961 tagattggcc ggtcgcctcc gtctgccttc acgcccgcgc gacgaagcgc ggatgaccct
     1021 gatcgagcac ctcgatgagt tgcgctccag gatcatcaag gtcggtgtgg cgttcgttct
     1081 ggccgccacg gtagcgtggt tctttcgcga acagatcttc acttttctca tggaacctgc
     1141 gggagaccgg ctacacggga agctctacgt cacctcggtg gcggagcagt tcatcaacga
     1201 cctgaagctg gcgctctacg tggcgttcgt ggtcacgata ccgatctttc tctaccaggc
     1261 gtgggcgttc gtcgcaccgg cggttggcga cgtgggccgg gttttcacct acatccttat
     1321 cacgctcgcc tcgtcgttgt tcgttgcggg gatcgccttc gggtacttct tcgtgctgcc
     1381 cgtcggtgtg cagttccttc tcggctggga cactagccgg tatataccga tcatcacgcc
     1441 gacgaactac ctggccttcg tcacgcgctt cctactggct tgcgggatag tgttcgagct
     1501 ccccgcggcg acctacgtcg gggcgaagct cgagctgatc gacgcctcgt tgctgaagaa
     1561 gtaccggcgc cacgccatcg tggcgaacac gatcctcgcc gcggcgctca cgcccacgcc
     1621 cgatcctttc agcatgatcc tcctggcgat acccatgatc ttcatgtacg agctctccat
     1681 cgtcatcgct cgatacgtga acccgacacc cgaagtagcc gttcatgaac ttgcggccaa
     1741 cgaagacgaa gacgatgact acgacgatta cgacgaagac gacgattacg acgaagatta
     1801 cgaagaggtc gagccggtct acgacggcgt cgagcgacgc gatgacgacg acgaagatga
     1861 cgagaccgag cgggatctgt agtttatagg cttcaggtct cagcttattg cgagcgccaa
     1921 ctccgcgctc ttagctaccc gatccgtcgc gccgctgcgt atcgaacacg gcgatacatt
     1981 gcgcttacct ctacctgtag cctgagacct gaagcctata acagccagcc gaggagcact
     2041 ccggcggtgt aaagcaatcc gaatacgagg tggagcccgg cggtgcgctt gacgacgggg
     2101 tcgagacgct ctggcgtcgt ggtcgtcgcg atgtctcgcc agagccggat ggcgggggtg
     2161 gcgctgagga agacgacggc gcactccagc gggacgatac gcaaggccat caacaccgcg
     2221 accgcgacgt acgcctccag cagcaggacg cggtagagaa ggatcgcaac ctcccggccg
     2281 agcacgatcg gcagcgtccg acgcccgccc cgcctgtccg acctcatgtc gcggatgttg
     2341 ttggccagca ggatggcggc cactggcccg cctatcggga gcgccgccag cgggacgtac
     2401 gcgggaaagc ttcccgggat ctgcaccgcg taagctatga cgactatgag gacgcccatg
     2461 aagaggaaga ccgcggcctc gctcaccggc gtgtaggcga tgggccgcgg gccggcggag
     2521 tagaggtagc cgcccagagc gcacagacac ccaagggcga gtatctgcca gccgcccacc
     2581 gcgacgaggt agatgcccag aacgagggca actgtgtagc agaacaacgc tcccagcagg
     2641 acggcccgcg cgcgcatccg tccacggacg atggagccgg cgatcccgac cgccgtcgca
     2701 gtatccaacc ccctctgctc gtcgtagaac tcgttgaaca tgttcgtggc ggcctgtatg
     2761 agcaggctcg cgaagagcat cgcaagaaac ggtccccacg cgaatgcgcc atccttgagt
     2821 gcgagcgccg tcccgaagag cacgggtacc gctgatgccg tcaaagagaa gggccgcgcc
     2881 agccggaacc actcgctgaa gatggaacct ctggggttcg tcgcgttcaa cggctactcg
     2941 gcgagtgatg gtttatgggc tcgaacgccc caacgagata tccccacccc tcgtctcgac
     3001 ctccgaattc ggacatggct cctttctagc cgtacgaccc gggtaattct gtaagcgctc
     3061 gcgggctttt tcaacagagg tcacaggcta cagcaaacta cctgattcct gaggctgact
     3121 ggctgtcgag ccgacaaact gatataaccg gcagtcgtgg aagcgggcgg cactatgggg
     3181 atagctggta cttacctccg gctggtccgg cccaagcagt ggaccaagaa cgggttcgtg
     3241 ctggccgggg tggtgttcgc gcacgaggcc cttatggtgt cctcggtggt gaaggcgttg
     3301 ctcgccttcg ccgccttctg cgctctctct ggcgccgtgt acgctgccaa cgacgtgttc
     3361 gacgtcgagg aggaccgcaa gcacccacgc aagaggagcc ggccagtggc gagcggcgag
     3421 atctctccgc gctccgccct cgtgttcgcc gccgtgctgg cggccgcagg actggggttg
     3481 gggttcgctg tcgacctcgg cgtggggctc gcggggattg cctatcttgc ccttcaggcc
     3541 ctctacacga cgacgctcaa gcacatggcg atcctggacg tcatgtgcat ctcggccggg
     3601 ttcgtcatca gggcgctcgc cggagtggcg gcggtgagcc gcccgatctc accctggctc
     3661 atcgtgtgca ccgcgctctt gacgctgttc ctcggcttct ccaagcggcg ccacgagctg
     3721 gcctcgttgg gcgaaggggc gacgatccac cggcggaacc tggcggatta ctccgtgccg
     3781 ctcctggacg agatgatgaa catcatgatc tcggccacca tcatcgccta cgccctttac
     3841 accttcttcg agtacgacaa ctactacatg atggcgagca tcccgttcgt ggtctacggg
     3901 gtgttccggt acatgctgct ggtccaccgc aacggcggtg gcaacccgga caccctgctc
     3961 ctggcggacc gtccgctgca gatcgcgctc ctcctctggc tggtcgtggc cggagtggtc
     4021 atctacgtca tccccttgtg gaacggctga agcacaacct catcctgtcg ctcgccctcg
     4081 gcgtcgccgt atacctcgta cttgccgtcg tgagcggctt cgggagcctc accgccgccc
     4141 tggaggactt cgacctcgcg ctcgtaccgg cgatcctcgg cctcgtgctc ctctcttacg
     4201 cggggcgttt cctgcgctgg ctttactacc tgaagttact gaaggtctcc gtcccgctac
     4261 ccacgaacgc tgcgatcttc gccgcggggc tctcgatgac tatctcaccc ggcaagctcg
     4321 gcgaggtgct caaaagcgtc ttcatccgcc aggcctcggg ggcg
//
LOCUS       DASTUV010000124         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1567205, whole genome shotgun sequence.
ACCESSION   DASTUV010000124 DASTUV010000000
VERSION     DASTUV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567205"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..694
                     /locus_tag="VFT03_02560"
     CDS             320..694
                     /locus_tag="VFT03_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4847055.1"
                     /translation="MSRPDGEVTMIYIVNKDDLPQSKIAHRFEGYRYGDVDVSVFLVD
                     SPPGGGAVLHTHPYQEIFITLEGNATFTVGGDTIEVSAGQIVVAPAGVPHKFVNSGDG
                     PLRQVDIHPSGHIQQVNIPEDS"
     gene            887..1087
                     /locus_tag="VFT03_02565"
     CDS             887..1087
                     /locus_tag="VFT03_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847056.1"
                     /translation="MADVTYVSRVRVEPVEGRVRRAYIPGREEPVLFGVHSEVAEHYG
                     VSPDVEEPHSSTLDFLVAAAGG"
     gene            1088..1351
                     /locus_tag="VFT03_02570"
     CDS             1088..1351
                     /locus_tag="VFT03_02570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU4847057.1"
                     /translation="MLGTFRGALVARQISYEDLSAETVGEIETEDKVLVLKRIKQHFH
                     LTAGEEDRATIERVLKVYADSCPVARSIKNSIEISSELDFTAS"
     gene            1473..1898
                     /locus_tag="VFT03_02575"
     CDS             1473..1898
                     /locus_tag="VFT03_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847058.1"
                     /translation="MRGRVRQRVQQRPQQRPQQRPQGRQTRGSESRRGPAFDVDEIEG
                     YARRLKDEVGDNPGLTGAASGLAGAASGLAGGALAHGLTGGGASEGDPGDELRERLDL
                     MEERLQRLEDEVRALREGGESAPEAPTEPGPAPDPEGNL"
     gene            complement(1917..2354)
                     /locus_tag="VFT03_02580"
     CDS             complement(1917..2354)
                     /locus_tag="VFT03_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847059.1"
                     /translation="MTDSTDGATFQELFSVIQDYAQRDHNHQVKALRVIAAAYLPLYD
                     LPPMPDAREVVEDVLTRNDFLVTDPQSSRLEPASVDAVVSVATSRLDGEDIRWGAGCL
                     LNVMDALRRRAQSEGYETYVLDADEVLDGLETILAADIVEDVL"
     gene            2844..3062
                     /locus_tag="VFT03_02585"
     CDS             2844..3062
                     /locus_tag="VFT03_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847060.1"
                     /translation="MNGSWHYMMEDRGLRQQDLAQRVAERESYARSKHKSSLRARIAR
                     RLFDLAVAAEREETWRLVWERLEARGRL"
     gene            3108..>3392
                     /locus_tag="VFT03_02590"
     CDS             3108..>3392
                     /locus_tag="VFT03_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4847061.1"
                     /translation="MNALELRNVRKSFKKRRGEVVHALDSVSLAVGRGEVVGILGPNG
                     SGKSTLVRVVSTLVVPDAGSVEVFGVDAVRHPKKVQRSMNRVSVEASFFKK"
BASE COUNT          684 a         1000 c         1169 g          539 t
ORIGIN      
        1 atcgcttcgg gaagttccct gggacggtgc cgagcatgtt catcctcacg tatctgcagg
       61 cgcatcacct ggacctggag ttctacgacc accacttcgg ccctggagcg tacctgaaaa
      121 cccacgcaga ccacatgtct cgctggcatc cagaaatgag cggggggtag agacccagag
      181 aatcccgcag gacatcccct tctccgccac gatgggggag tagccggagg tagaggtggt
      241 gtcgatctgg ctattggacg ctcaggtcct cggtcctata ctccctagag caagtagcga
      301 gccgggcgat gaggcccgca tgtcgagacc cgacggagag gtgacaatga tctacatagt
      361 caacaaggac gatctccctc aaagcaagat cgcgcacagg ttcgagggat accgctacgg
      421 cgatgtggac gtatctgtct tcctggtaga cagcccgccc ggcggcggag cggtgctgca
      481 cacccatcct taccaggaga tcttcataac gctggagggt aatgctacct tcaccgtcgg
      541 cggtgacacc atagaagtga gcgccggcca gatagtggtg gctccggccg gcgtgcccca
      601 caagttcgtc aactccgggg acggaccgct gaggcaggtc gacatccatc ccagcggaca
      661 catccaacag gtcaacatcc ctgaggattc ataaccacct gattacgccg cgcaggctcg
      721 ggagcaggtg gtcgaaggcc aactgcaaga aagtcacggt ggatgaggag ccggaggtgc
      781 cggcggattg aagccgggcg gcgccagtac gcgggctccc gaccggtagc ctgagcgagt
      841 ccgccctata ctacactcag gagcccagat aagcgggagg aagagtatgg ccgacgtaac
      901 gtacgtctcg cgggtaaggg tggagccggt agagggcagg gtccgccgcg cctacatccc
      961 cggcagagag gagccggtcc tcttcggcgt acacagcgag gtcgccgagc actacggagt
     1021 ctcgcccgac gtggaagagc ctcattcctc cactctcgac ttcctcgtgg ctgctgcggg
     1081 cggctgactg ctcggaacct tcaggggcgc gctggtagcg cgtcaaatct cttatgagga
     1141 tcttagcgcc gagacggtgg gggagataga gacggaagac aaggtgctcg tgttaaagag
     1201 gatcaagcag cacttccacc tcaccgccgg cgaggaagac agggccacta tagagcgggt
     1261 actgaaggtc tacgctgaca gctgtcccgt agcccgctcg ataaagaaca gcatcgagat
     1321 ctcgagcgag ctggacttca ccgcgagcta gcgccaccgg cggtgggtta tgcccgatgc
     1381 cctgcgggta cagcttatgg cacgagacca gggaggcaga cgtgggcaga agggacagag
     1441 gcagggggtc acaggggcgc gcggaggacc gcgtgcgagg gcgagtgcgg cagcgcgtac
     1501 agcaacgccc gcagcaacgc ccgcagcaac gcccgcaagg acgccagacg cgcgggtcgg
     1561 agagcaggcg aggccccgcc ttcgacgtgg acgagataga gggctacgcg cggcggctca
     1621 aagatgaggt cggagacaac ccggggctca ctggagccgc ttccgggctc gccggagcgg
     1681 cctcggggct ggcggggggc gcgctggcgc acgggctcac cggcggcggt gcttcggaag
     1741 gagatcccgg ggatgagctc agggagcgcc tggacctgat ggaggagcgc ctgcaacggc
     1801 tcgaggacga ggtccgcgcg ttgcgcgagg ggggagagag cgcccccgaa gctccgacgg
     1861 agccaggccc ggcgccggac cccgaaggca acctgtagcg ctcctgcggg tacgttctaa
     1921 agaacgtcct ctactatgtc ggcggcgaga atggtctcca ggccgtcgag gacctcgtcg
     1981 gcgtctagca cgtaggtctc gtatccctcg ctctgcgccc tgcgtctcag agcgtccatc
     2041 acgttgagga ggcagccggc tccccaccgg atatcttccc cgtctagccg ggaggtggcc
     2101 acgctgacca cggcgtccac gctggcgggc tcgagccggc tgctctgtgg atcggtcacc
     2161 aggaagtcgt tgcgcgtgag gacgtcctcc accacctctc tggcgtcggg catgggggga
     2221 aggtcgtaaa ggggcaggta ggcggcggcg ataaccctga gcgccttgac ctggtggttg
     2281 tggtctctct gcgcgtaatc ctgtatgacc gagaaaagct cctgaaaggt ggctccatcc
     2341 gtggagtccg tcatctgatg ccctccttgg cgcctgtaat gctccgacgc gccatgtata
     2401 ccccgggcac cgcgaagcta cccgcgcaag actctcgaaa aatcactcaa atgggtgatg
     2461 cgccgcgcct gcacggctgg cagaatattc tccgggtctc ggctcgcggc gaagggcgga
     2521 ctacgttgtt gacaacgccc tcgcggacgg gtagagtgcc cgggttgtgt tgtagtgtcg
     2581 cgaaaggact gaagggacag atgcaaatcc gtgtcacgca catagctgga atcccggttt
     2641 tcgggggaga ggtgcgtccc gaggtccgca cggtcgcgat gagaacgtga gcctttagtt
     2701 cacacagatt tctcaaagag gcccccggac accaggaccg ggggccaaat cttttgcttg
     2761 aaaagcaacg gtccccatcc ttcccgagac gggcatcacc cgcgagcagc cgtcgcgacg
     2821 agagaagaag aaggagaccg gggatgaacg gaagttggca ctacatgatg gaggatcggg
     2881 gtctgcgtca gcaggacctc gcccagaggg tggccgagcg cgagagctac gcgcgctcga
     2941 aacacaaatc atccttgcgg gcccggatag cgcgccggct cttcgatctg gcggtagccg
     3001 ccgagcggga ggagacctgg cgcctggtct gggagaggct ggaagcgagg ggccggttgt
     3061 agagccggcc ccctcgcggg ggaagtagaa aacgaaggga attgctcgtg aacgctttag
     3121 agctacgcaa cgtacggaag agcttcaaga aacggcgggg cgaggtcgtg cacgccctag
     3181 actcggtctc gctcgcggtc gggcgtggcg aggtggtagg catcctcggg ccgaacggca
     3241 gcggcaagag cacgctcgtg cgcgtggtct ccaccctcgt cgtacccgac gccggctcgg
     3301 tggaggtctt cggcgttgac gccgtgaggc acccgaagaa ggtgcagcgg agcatgaaca
     3361 gggtcagcgt cgaggcgagc ttcttcaaga ag
//
LOCUS       DASTUV010000125         3887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1702423, whole genome shotgun sequence.
ACCESSION   DASTUV010000125 DASTUV010000000
VERSION     DASTUV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702423"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /gene="xylB"
                     /locus_tag="VFT03_02595"
     CDS             complement(<1..1408)
                     /gene="xylB"
                     /locus_tag="VFT03_02595"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEU4847062.1"
                     /translation="MLLGLDLGTSSVKALLMAGDGDTIGEGSASYPVRSPRPGWAESA
                     PEDWSNALASATRAAVGPHGAEVVAIGLSGQMHGVVLTDDRGRPLRPAILWADTRSTR
                     ELAAYRGLCEEAEERLANPPAVGMAGPTLLWLRDHEPDTYASARWALQPKDWLRLRLT
                     GEAAAEPSDASATLLYNLLSDDWDYAVVEALGLHAGLLAPLVPSAGVAGTLTREAARS
                     LGLRTGLPVAAGAADTAAAMLGGGILRPGLVQLTVGTGGQIVAPKSAPEPDPYRRTHL
                     YRAATPDLWYSMAAIQNAGLALEWARATLGASWREVYEEAFAVPPGAGGVVFLPYLSG
                     ERTPRFDPEARGAWVGLGLEHGRGHLLRAALEGVAFALREGLEALEEAGTAVSELRLA
                     GGGTAEEPWRQLLADVIGRPLRVLPGGVASVASARGAALLAGAASGVYRSWQDVLALA
                     PEPDHSVRPGGAGGTYDTV"
     gene            complement(1645..2298)
                     /locus_tag="VFT03_02600"
     CDS             complement(1645..2298)
                     /locus_tag="VFT03_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEU4847063.1"
                     /translation="MKNLILIVAALATVVALAGCGSSGQSGNDQQGDQQNQQKEQKAQ
                     QQKTPDKQVAPEKGTQDSGDEQAAVDPPSNDMMKLTVPKMEMIKDDEIPTGLGTDDTL
                     FHDYAGVHIEHTGFPWDKEANVYIAGHRLGFPNTDSHLAFWDLNKLEDGDEFYITDSE
                     GRKYTYKVFNKFIASPENLSVLAPVDGKNIVTLQTCTLPDYTSRLLVRGELQGITES"
     gene            2649..2900
                     /locus_tag="VFT03_02605"
     CDS             2649..2900
                     /locus_tag="VFT03_02605"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEU4847064.1"
                     /translation="MPLYMDHHKHVEGLTAEAVADAHAKDLEAQEKYGVNYIRYWFNE
                     EKGEVFCLVEAPNAEAADAVHKEAHGLGADEITEVKEGS"
BASE COUNT          686 a         1239 c         1271 g          691 t
ORIGIN      
        1 agaccgtatc gtaggttccg cccgcgccgc cgggccggac ggagtggtcc ggctcagggg
       61 cgagagcgag gacgtcctgc cacgaccggt agactcctga ggcggcgcct gcaaggagcg
      121 cggcgccacg ggccgacgcc acggaggcta ccccgccggg cagcacccgt agcggacggc
      181 cgatcacgtc ggcgaggagc tgccgccagg gttcctcggc ggtgccgcct ccggcgaggc
      241 ggagctcgga gaccgcggtc cctgcctcct ccaacgcttc gagcccctcg cgtaacgcga
      301 acgcgacacc ctcgagggcc gcgcgcagga gatggccgcg tccgtgctcc aggccgagcc
      361 cgacccaggc tccccgcgcc tcggggtcga aacgcggcgt ccgctcgccg ctgaggtacg
      421 gcaggaagac gacgcccccg gcgcctggcg gtacggcgaa agcctcctcg tacacctcgc
      481 gccaggaggc gcctagagtg gcgcgggccc actcgagagc cagccctgcg ttctggatcg
      541 ccgccatcga gtaccagagg tccggcgtgg cggcgcggta gaggtgggtg cgcctgtacg
      601 ggtccggctc gggagcgctc ttcggcgcca cgatctggcc tccggtcccg acggtcagct
      661 gtaccagccc tggccgcagg atccccccgc cgagcatagc ggctgccgtg tccgccgccc
      721 ccgccgcgac cggcagcccc gttcgcaatc cgagagatct cgcggcctcc ctggtgagcg
      781 tacccgcgac cccggccgaa gggacgagcg gagcgagcaa ccccgcgtgc aggcccagag
      841 cctcgaccac ggcgtaatcc cagtcgtcgg agaggaggtt gtagagcagg gtcgcggagg
      901 cgtcggaggg ctcggctgcg gcctccccgg taagtcgcag ccgcagccag tccttgggct
      961 ggagcgccca gcgggctgaa gcgtatgtat cgggctcgtg gtcgcgcagc cagaggagag
     1021 taggcccggc catgccgaca gcgggtgggt tggcgagccg ctcctcggct tcttcgcaga
     1081 gtccacggta ggctgcgagc tccctggtcg agcgcgtgtc ggcccagagg atggccggcc
     1141 tcagcgggcg cccgcggtcg tcggtgagga cgacgccatg catctggccc gagaggccga
     1201 tcgccaccac ctcagccccg tgcggcccga cggcggcccg cgtggcagag gccagggcgt
     1261 tactccagtc ctccggggcg ctctcggccc accccgggcg cggcgagcgg acggggtagg
     1321 aggcggatcc ctcgccaatg gtgtcgccgt ccccggccat gagtagggcc ttgacggagc
     1381 tggtgccgag atccaggccg agcagcacgg tggtcagtcc ctccggcgcg gcacgtcgcg
     1441 gggattactc gaacgggccg cgccaggtcc ccttcgcgcg caggaaggcg gggccgccgg
     1501 tcaccagggc gtccggctcg atgaggtgtt cagttccccg atcctccttc gtcggacctt
     1561 ctcccggttc gaatagtaaa gccagagggc cggggccaac aagccccgac cctcttccgg
     1621 cgcgtccgtg gtgttgaccg gtctctacga ctccgtgatg ccctggagct cgccgcggac
     1681 gaggaggcgg ctagtgtagt ccggcagcgt gcaggtctgg agggtaacga tgttcttgcc
     1741 gtccacaggg gcgagcacgc tgaggttctc cggcgaggcg atgaacttgt tgaagacctt
     1801 gtaggtgtac ttcctgccct cggagtcggt gatgtagaac tcgtcaccgt cctcgagctt
     1861 attcaggtcc cagaaggcca ggtggctgtc ggtgttcggg aagcccaagc ggtggcccgc
     1921 tatgtagacg ttggcctcct tgtcccacgg gaagcccgtg tgctctatgt ggacgcccgc
     1981 gtagtcgtgg aaaagcgtat cgtcggtgcc gagcccggtg gggatctcgt cgtccttgat
     2041 catctccatc ttcgggaccg tgagcttcat catgtcgttc gacgggggat cgacggcggc
     2101 ctgctcatcg ccggagtcct gcgtgccctt ctcgggcgcg acctgcttgt ccggggtctt
     2161 ctgctgctgc gccttttgct ctttctgctg attctgctga tcgccctgct ggtcgttccc
     2221 ggactgcccc gaggagccac agccggccag cgccacaacg gtcgccagcg ccgcaacgat
     2281 cagtatcagg ttcttcaagc gcatctcatc acctctcgtc tttttcacag gacgcctaag
     2341 atcccgactt accactaaaa cataccgacc aaaaataaca agatggttac gttttgatca
     2401 aaggtccgtt acactgcctc gccgcgtcgt gccggccccg ttttccggag catatattag
     2461 ggtctgtcct cccaggtgta aaggctcgct gcagtatcgg cagcgtgcca ggccggcggc
     2521 agaagatagc gagcggccct atttcacgcg agcggtcctc cagaggtgca ccggccctcg
     2581 cctcaggtta tgatgtcgct gttgtaacag agccgcccgc tggggaaagc ggggagagga
     2641 ggacagtggt gcccctgtac atggaccatc acaagcacgt ggaagggttg accgcggagg
     2701 ctgtggccga cgctcatgca aaggacctcg aagcccagga aaagtatggg gtgaactaca
     2761 tcaggtactg gttcaacgag gagaagggcg aggtcttctg cttggtggag gctccgaacg
     2821 cagaagccgc agatgccgtc cacaaggagg cccacggcct cggagcggat gagataaccg
     2881 aggtcaagga aggatcgtag caaggggcga atgatgccag gccaggtacg cggcactgga
     2941 cagcaagctg cttgcagatc caccccattc cttgaacgct aacccttctc gaaaagttcg
     3001 atcccggcaa agcctcggag gatttagaca agccaagagg gacgagagca cagcttattc
     3061 gctgaagctc gtggaaggta ttcttagaag tccgcgtagc cccggcccgg tgtcccatgc
     3121 ccctccagga ctgagactct attgtgaaca cacaagccgc ccataatcac ccgcgatagg
     3181 cactagctct ccggtggtcc tggccgctct gaaccagggc acagcgactg cccttcggca
     3241 cttagtgctg tgtgtcactt gcattctatt cacacaggag tagactgcgt tccttacttg
     3301 gcgaactgac gccatgtaaa gggacagggc ggctagctgc cctgtcccta agaatgagtg
     3361 gctacttaga aaggagggga agagatgccg gtagagggta cgcgtcgacg tagttgtcgt
     3421 agttgccgta gacgctgtcg ccgcaaggat agccgcagtg agcgcaggcg ttgtcaacgc
     3481 cgttgccgcc gccgctgcga tcgtcgtgac agatagaagc cacgcaagct aggttaggtt
     3541 gtaggaaggc cccgttagtt gcggggcctt cttctcgtat attctcgcac aacctcctgg
     3601 ccgtttactt cccttcttgt ccttatgcac caacgtctca taaggagcgt tctgagaagt
     3661 ccaaatccag gatcgtgcat agtcccggtc ctggaggcgc gaagatcgcc tttgaggacc
     3721 tccggtggcg ctcccgacca tgccctcgct actcggttgg atagatattc gcagtcctag
     3781 attactgtac aaccgcccta accgtgagcg tagcaatgtt tgtgcctggt gtacagaact
     3841 ccgagtagta gcatcacaga caaggacacg agccgcataa atccctt
//
LOCUS       DASTUV010000126        10171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1273543, whole genome shotgun sequence.
ACCESSION   DASTUV010000126 DASTUV010000000
VERSION     DASTUV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273543"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2627)
                     /locus_tag="VFT03_02610"
     CDS             complement(<1..2627)
                     /locus_tag="VFT03_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4847065.1"
                     /translation="MRRTAIRETLLFWRSHEEPGRAGEASEKDAGVLRALAGVVFEGV
                     AVVEEGGRISEANEIFASVFGYELPEVVGRTLEEFVIPEFRGLVEKSLSAGFGETYEA
                     VAMKRDGTPLNVEIRSQPSSYGGRVVHLVAVRDIIGDNGVEGRLGQAGYRALVEQIPA
                     VTYTQEPKAGHHFASLYLSPQAERMLGYTLDEFASDPGLWVRLIHPDDRERVLAEDAR
                     TDETGEQFSAEYRLLTRDGRMVWVRDEATLVSDEDGRPLCWQGVLTDVTDRKQAEEAL
                     KESEQRFRSLVQNSSDIITLVGADGTILYVSPAIERVLGYRPEERVGSNTFELVHPDD
                     VARARRLFARDLRNPGATSSIGVRVRHRDGSWRYVETTGTNRLDEPGVEAVVLNSRDV
                     TEREWTEVALKESERRYADLLSSTRAYIYRCLNEPGWPIEFASAYALELTGYAPEKLM
                     VGGTVRFGDLVVEEDRQRVWDEVQTALQRRERFKIRYAIRRRDGTLRHVEQYGQGVYD
                     QEDNVEAIEGIVYDVTELKRAETRLREAEERYRTLVEEIPAVTYIKAVDDSSQSSYVS
                     PQIEDMLGYKPEEWTSDPDQWIKIMHPADRERVLAEVARTDETGEPFRMEFRQFAKDG
                     RVVWVRDEAVLVRDEEGNPSYWLGFQYDITKRKRSEEARKESELRLRTVISNVPVVLF
                     AINFAGVISLSEGKGMGALGLEPGEVVGRSISEVFGGVPGILEDVDRALAGETLSAVR
                     EVDSRTFEMWYSPVRANTGEVTGVTGVSIDITDRKRAEEELKESAGRFRSTFENAPIG
                     MALVSLDNHYRQVNQAFCDMLGYSQEELLSRRTFDITHPDDLEASIARTRALLEGTVE
                     RDLLEKRYIRADGDIVWALS"
     gene            complement(2906..4195)
                     /locus_tag="VFT03_02615"
     CDS             complement(2906..4195)
                     /locus_tag="VFT03_02615"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4847066.1"
                     /translation="MSRLLLRARGMAVLRGVLASPVSKYFLALLGLLAARRPDPAAVA
                     GTFGRLWEDLALERERLLPDAWRSHLVGRLLDDENAFSLAAERGEASPALVEQASRDL
                     RTLRRLFDLEAEPLLNVVEDAVPGLEGVWGPWTDPERGDTSSPRHALARKLEAAEDWG
                     RCAGLLADHFSRHGAGSFGRHGAFRWGEDGLRAVDEPDPVRLADLVGYERVREPLLTN
                     TERFLAGLPAHHALLYGMPGTGKSSTVKAVMNEYTDRGLRLVEVAKEDLGALSRVLES
                     LRARAPRFVIFVDDLSFEENEVEYKALKALLEGSVEEPPDNVRVYATSNRRNLIRERF
                     SERDESGVGDDVHARDTMQEKLSLSARFGLRVTFPTPDQGQYLEIVAGLARRRGIEMP
                     DEELKERAILWDRWHAGRSGRTARQFVDELQAELSGG"
     gene            complement(4316..4999)
                     /locus_tag="VFT03_02620"
     CDS             complement(4316..4999)
                     /locus_tag="VFT03_02620"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU4847067.1"
                     /translation="MAGTNFPKVWAYMLAGVLAAGLLVATVFAPGAAAQDPGCKGDIV
                     LDPGHGGNDTGAVNKKYNLKESEEVLKVAGLLKALLEADGHKVCMTRTTNSETLSNNN
                     RYTYANTTGAEILVSIHMNGSSNTATDYTTILFGKWRKDKELAYALLDDLSALPATSG
                     TGTIATRDPYSFASGVLLKSDMPATIAETVFITNDKEGQLLSNGTGARQQQIAEALRK
                     GIEGYLSTH"
     gene            complement(5138..5740)
                     /locus_tag="VFT03_02625"
     CDS             complement(5138..5740)
                     /locus_tag="VFT03_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847068.1"
                     /translation="MEGEERIGLVGAATVLDVPEEAVRRLAEEGRLEAERGEDGAYSF
                     RLSEVVDLSRELGAEEIFEGEVIGDEPGPSRLPAVPLERYERLLQQGQDYKTRLEERS
                     RHMEEIRKERDAARMEIDRLWSEIERLNLVEFQRELQERTISTLEEEKAKLESERERL
                     IQERLELVEEKSSLVEDRVRLEAELKALKSRGFWARLFGG"
     gene            complement(6002..6541)
                     /locus_tag="VFT03_02630"
     CDS             complement(6002..6541)
                     /locus_tag="VFT03_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEU4847069.1"
                     /translation="MEEKISRGRFIRLGTALGVGAASASVLAACGGGGTESGGGGGSG
                     NGSGGGGNGGNDGGGYGGNAGGGEQAAGGGSKKPKDPGGKAIAETSEVAPGTALQFKD
                     SGSPAVLVHLDSGDFVAYSAVCTHQGCTVAYKNGELACPCHGSVFDPAKGAEVVAGPA
                     PRPLPKIPIKVSGGEVFMA"
     gene            6681..7163
                     /locus_tag="VFT03_02635"
     CDS             6681..7163
                     /locus_tag="VFT03_02635"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HEU4847070.1"
                     /translation="MVWVRVLVGAVWLNGAIEKLLNPEFPQQFAASLQAGGFVSQAPP
                     FFQEFMRSTVIPNAELFAQLMRAGELALGVALILGLLTNLAALGSVGLSIVIILSQGG
                     VRLGTGLGAPELLTINVVVALLSVVILLSAAAKDLSLDALLARRSAWLSPLLLNRRQV
                     "
     gene            complement(7235..8485)
                     /gene="lysA"
                     /locus_tag="VFT03_02640"
     CDS             complement(7235..8485)
                     /gene="lysA"
                     /locus_tag="VFT03_02640"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU4847071.1"
                     /translation="MDEFHHEAGVLRCEDVPLSEIAEGAGTPVYVYSYGALERAYREL
                     DGAFSGLDHMVCYAVKANGNLAVLRALASFGAGADIVSVGELYRAMRAGFDPKKVVFA
                     GVGKTERELMAGLGERILLFNVESASELEHLDRLASRHGKRARIALRVNPDVDAGTHE
                     HVATGRAFDKFGIPVDEALALAERVGQYRSVDLIGVHQHIGSQITKLAPFTESVEKSA
                     TLVDELKNRGFDIRYFNMGGGLGIRYKDEEAPSPEELVAAVRPTLEATGTKILCEMGR
                     YIAGGAGALLTRVIYRKKSGEKDFIVADAGMNDLLRPSLYDAHHEVRAVSEDGSVANA
                     DLVGPVCESGDYLARDRELPDAREGDLLAVMCAGAYGFSMASNYNSRPRPAEVLVKGD
                     RWAVVREREGHADLVKGELVPAFL"
     gene            8601..9803
                     /gene="dapC"
                     /locus_tag="VFT03_02645"
     CDS             8601..9803
                     /gene="dapC"
                     /locus_tag="VFT03_02645"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEU4847072.1"
                     /translation="MKGRRRLGRTSFPPLLLNPALERSGAYPLLRLEERRREVRERGV
                     ELFDFGTGDPREPTDPKIKRALVDGVPEVSQYPSAQGKRSLREAFAGWMERRNGVALD
                     PEAEVLPATGSKEAIFHAPLAFLHPSHERRGVAYGTPGYPVYERGTLFAGGEPVPLRL
                     KAGDEFLLRVEELDPQNIRALWINYPHNPTGATAPYEYLEKVAAFCRAHDILLFSDEC
                     YNDLYFSDPPPSILEITRERTLAFCSLSKRSGMTGYRSAMMAGDAELIAALKKLRPSI
                     GVATQSFVQDAATAAWNDDVHVEKRRQIFAEKRELFKDFFERAGLRYTRTDASIYLWI
                     TVPEAFGGDDEAYALRLLEEGIVVAPGRSFGPGGEGYVRVALVPRLEECRKAIEKWEG
                     LIRRDFSA"
     gene            complement(9795..>10171)
                     /locus_tag="VFT03_02650"
     CDS             complement(9795..>10171)
                     /locus_tag="VFT03_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847073.1"
                     /translation="AYTIWDKHAVSALLIPPLLQSWVTTLVTTGLLTPLAVRHRGKVL
                     AIWREHKIDALGVAVLSPLSYILVLTALVFTPVSYVAPAREISILIGAAMGASLLAEG
                     DVRRRLVAATVMVVGFAALALG"
BASE COUNT         1629 a         3522 c         3168 g         1852 t
ORIGIN      
        1 gatagagccc acacgatgtc cccgtcggca cgtatgtagc gcttctccag gaggtccctt
       61 tctacggtgc cttccagcaa tgcgcgcgtg cgggcgatac tggcctcgag gtcgtcaggg
      121 tgggtgatgt cgaatgtcct cctggagagc agctcctcct gggagtagcc aagcatgtcg
      181 cagaacgcct ggttgacctg gcggtagtgg ttatcgaggc tgacgagagc catgccgatg
      241 ggagcgttct cgaaggtact gcggaagcga cctgcgctct ccttcagctc ctcctctgcc
      301 cgcttgcgat ccgtgatgtc tatggagacg ccggtcacgc cggtgacctc gccggtattc
      361 gcccgcaccg gcgaatacca catctcgaac gtccgcgagt ccacttctct taccgcgctg
      421 agcgtctcgc ctgccagggc gcggtccaca tcctccagta tccccggtac gccgccgaac
      481 acctccgaga tcgaacgtcc aacgacctcg cccggctcga gccccagagc gcccattccc
      541 tttccctcgg agagtgagat cacgcccgcg aagttgatgg cgaagagcac gacgggcacg
      601 ttcgagatga ccgtgcgcag cctgagctcg ctctctttgc gcgcctcctc cgagcgcttg
      661 cgcttggtga tgtcgtactg gaaccccagc cagtagctcg ggttgccctc ctcatcccgg
      721 accagcacgg cttcgtccct gacccacacc acccgaccgt ctttggcgaa ctgccggaac
      781 tccatcctga agggctcacc tgtctcgtcg gtgcgggcta cctccgccag cacccgctcg
      841 cggtctgcgg ggtgcatgat ctttatccac tgatccgggt ccgaggtcca ctcctccggc
      901 ttgtagccga gcatgtcctc tatctgcggg cttacgtagg aggactggct actgtcgtct
      961 acggccttga tgtaggttac cgccgggatc tcctctacca gcgtgcggta cctctcctcg
     1021 gcctccctta gcctcgtctc agcccgcttg agctctgtga cgtcgtagac gatcccctcg
     1081 atagcctcga cgttgtcctc ctgatcgtag accccctgac catactgctc gacgtgcctt
     1141 agcgtgccgt ccctgcggcg gattgcgtac cggatcttga agcgctcgcg cctctggaga
     1201 gctgtctgca cctcgtccca gaccctctgc ctgtcttcct ctacgacgag gtcgccgaag
     1261 cgcaccgtgc cgcccaccat tagcttttcg ggcgcgtagc cggtgagctc tagggcgtag
     1321 gcgctggcga actcgatagg ccagcctggc tcgttgaggc aacggtatat gtaggctcgg
     1381 gtgctggaga gcagatcggc atagcggcgc tcgctctcct tgagcgccac ctccgtccac
     1441 tcgcgctcgg tgacgtcgcg cgagttgagc acgaccgcct ccacgccggg ttcgtcgagc
     1501 cggttggtgc ccgtggtctc tacgtaccgc caggagccgt cccggtgccg cactcgcacc
     1561 ccgatcgacg acgtggcgcc cgggttccgc aggtccctgg cgaagaggcg ccgggcgcgc
     1621 gccacgtcgt ccgggtgaac cagctcgaag gtattgctgc ccactctctc ctctggcctg
     1681 tacccgagca cccgttcgat ggccgggctc acgtagagaa tcgtgccgtc ggcccccacc
     1741 agagtgatga tgtccgatga gttctggacc agggagcgga aacgctgctc gctctccttg
     1801 agcgcctctt cggcctgctt cctgtcggta acgtcggtca ggacgccctg ccagcacaag
     1861 ggccggccat cctcgtcgct cacgagcgtg gcctcgtccc tgacccacac catgcggccg
     1921 tccctggtca acagtcggta ctccgcgctg aactgctcgc ctgtctcgtc ggtgcgggcg
     1981 tcctctgcca gcacccgctc gcggtcgtca gggtggatca gccttaccca cagaccgggg
     2041 tcggatgcga actcgtcaag cgtgtatccg agcatccgct ccgcctgcgg gctcaggtac
     2101 agagaggcga agtggtgccc tgccttcggc tcttgcgtgt aggtgaccgc cgggatctgc
     2161 tcgaccagcg cgcggtaacc cgcctgaccg agccgcccct cgaccccgtt gtccccgatg
     2221 atgtccctca ccgctaccag atggactaca cgccccccgt acgatgaagg ctgggacctg
     2281 atctcaacgt tcagcggcgt gccgtcccgc ttcatagcca cggcctcgta cgtctccccg
     2341 aatcccgccg agagactttt ctcgacaagg ccgcggaact cggggatcac gaactcctcc
     2401 agggtcctgc ccaccacctc cggcagctca taaccgaaaa ccgaggcgaa gatctcgttc
     2461 gcctccgaga tcctaccccc ctcttccacc acggcgacgc cctcgaagac gacgcctgcc
     2521 agagcgcgaa gcactcccgc gtccttctcg gatgcttccc cggctcgccc gggctcctca
     2581 tgactgcgcc agaagagtag cgtttctctg atcgcggttc tcctcacggc atccgattcc
     2641 ctaccttcga tcccgcgaac cccgccggct cccgcgagta cctattaccg ttgcgccgcc
     2701 ggttattgct tcatccgcta gccggtacga atgcgcaaga ccttcggatc aattgtaatc
     2761 gtattcaggt gtccaggcca acaccagcag gatagtgccg caacgggagg acccttcccg
     2821 ccgtgttgtg agcccgtccc tggcggtccg ttacctgtta cttggtggcc tgggcacggg
     2881 cgggacgggc actgctggag gaggtctagc ctcctgagag ctccgcctgg agctcgtcca
     2941 cgaactgccg ggcagtgcgc ccgctcctgc cggcgtgcca gcggtcccag aggatggccc
     3001 tctccttcag ttcctcgtcg ggcatctcta tgccgcgccg gcgggcgagg ccggccacga
     3061 tctcgaggta ctgtccctga tccggggtgg ggaaggttac gcgcagtccg aatcgcgccg
     3121 agagggagag cttctcctgc atggtgtcgc gggcgtgaac gtcgtcgccg actccgctct
     3181 catcccgctc ggagaagcgc tcgcggatca ggttccggcg attggaggtg gcgtagaccc
     3241 tgacgttgtc gggcggctcc tcgacgctgc cctcgagcag cgccttgaga gccttgtact
     3301 cgacctcgtt ctcctcaaac gacagatcgt caacgaagat gacgaagcgc ggcgcgcggg
     3361 cgcgcagact ctccagcacc cgcgacagcg ctccgagatc ctccttcgcc acctcgacga
     3421 gccgcaaccc cctgtcggtg tactcgttca tcacggcctt gacggtggaa gacttccccg
     3481 tccccggcat gccgtagagg agcgcatgat gcgccgggag gccggcgagg aagcgctcgg
     3541 tgttcgtgag cagcggctcc cgtacccgct cgtacccgac aaggtcggcg aggcgcacgg
     3601 ggtcgggctc gtccacggcc ctgaggccgt cctcgcccca gcggaacgcc ccgtgacgcc
     3661 cgaaggaacc cgcgccatgg cgggagaagt gatccgcgag cagccccgca cacctccccc
     3721 agtcctccgc cgcctcgagc ttgcgggcca gcgcgtggcg cggggacgag gtgtctccgc
     3781 gctcggggtc cgtccacgga ccccacacgc cctccagccc gggcacggcg tcctcgacca
     3841 cgttcagcaa gggctcggcc tccagatcga agagccgccg gagcgtgcgg aggtcccggc
     3901 tagcctgctc cacgagcgcg gggcttgcct cccccctctc ggcagcgaga gagaaagcgt
     3961 tctcgtcgtc cagcagacgg cccacgaggt gggacctcca ggcgtcgggc agaagacgct
     4021 cccgctccag cgcgagatcc tcccacagcc gcccgaaggt cccggccacc gccgcagggt
     4081 cagggcggcg cgccgcgagg agcccgagca acgccagaaa gtacttcgag acgggcgacg
     4141 cgaggacccc gcgcagcact gccataccgc gggcgcgcag cagcagccgg ctcatctcct
     4201 cacgctccat acagactgga actgtagcaa caaaggcttc aggctacagg aatcagaaaa
     4261 ggcccttaat atgtcgcgcc gccggaaggc cagcatgtac cgaagatcgg gggtgctagt
     4321 gggtggagag gtagccttcg atccccttcc tcagcgcctc ggcaatctgc tgctggcgcg
     4381 cgccggtgcc gttggagagg agctgtcctt ccttgtcgtt cgtgatgaag acggtctcgg
     4441 ctattgtcgc cggcatgtcg ctcttgagga gcactccgga ggcgaacgag taggggtctc
     4501 tcgtggcgat ggttcccgta ccgcttgttg ctggcagggc cgagaggtcg tcgagcagcg
     4561 cgtaggcgag ctccttgtcc ttgcgccatt tcccgaagag gatggtagtg tagtccgtgg
     4621 cggtattcga ggagccgttc atgtggatgg agaccaggat ctctgccccc gtggtgttgg
     4681 cgtaggtgta gcggttgttg ttggagaggg tctccgagtt ggtggtgcgc gtcatgcaga
     4741 ccttatggcc gtctgcctcg agaagagctt tcaagaggcc cgcaaccttc aggacctcct
     4801 cgctctcctt caggttgtac ttcttgttaa cggcgcccgt gtcgttgccg ccgtggccgg
     4861 ggtcaagcac gatatcgccc ttgcagcccg ggtcctgcgc cgccgcacca ggagcgaaga
     4921 ccgtcgccac gagaaggcca gccgcgagga ccccggccag catgtatgcc cacaccttcg
     4981 ggaagttcgt ccctgccatc cccatccctc ctagttaggg gcggaggcaa ggagctctcc
     5041 gtcccaggtt acgtaacgcg gggcagcata caggaggtaa ggggatgagc gtatccccca
     5101 aatgggctaa atttcgcgtg gataaccctt cgagcggtca gccgccgaag aggcgcgccc
     5161 agaagccgcg gctcttgagg gctttcagct cggcttccag gcggacgcgg tcttcgacga
     5221 ggctggattt ctcttcgacc agctcgaggc gttcctggat caggcgctcc cgctcggact
     5281 cgagcttggc cttctcttcc tcgagagtgg agatggtcct ctcctgcaac tcgcgctgga
     5341 actcgacgag gttaaggcgt tctatctcgg accagaggcg gtcaatctcc atgcgagccg
     5401 cgtcgcgctc cttgcggatc tcctccatgt ggcggctccg ctcctcgaga cgagtcttgt
     5461 agtcctggcc ctgttgcagg agccgctcgt agcgctccag cggtacggcc ggcagcctgg
     5521 agggaccggg ctcgtccccg atcacttctc cctcgaagat ctcttccgcc ccgagctcgc
     5581 gggagagatc cacgacctcg gagagcctga agctgtaggc gccgtcctcg ccgcgctcgg
     5641 cctcgaggcg cccctcttcc gcgaggcgcc ggaccgcctc ctcggggacg tccagtactg
     5701 tggccgcccc aaccagcccg atgcgctcct caccctccat gcctgaatat tagctttagc
     5761 acgctggctt cgccagcttg tcagcacgct ccggctaacg cctccgcttg tcagcacgct
     5821 ggcttcgcca gcttgtcagc atttcagctt actcacttcc gttcggctca tgcccggtcg
     5881 cgcttggaga gacccataag cttggactgg cgcagggaag caccatctcg caccctcctt
     5941 gcttttagct gacaagcgaa ggcgccagcc ggagcgtgct ggcttgattg ccgaagggca
     6001 atcaagccat aaacacctct ccgccgctga ctttgattgg tattttcggc aggggccgcg
     6061 gggcggggcc ggccacgacc tccgcgccct tcgccgggtc gaagacagag ccgtggcagg
     6121 ggcacgcgag ctccccgttc ttgtaggcta cggtgcagcc ttggtgcgtg cagacggccg
     6181 agtaggccac gaagtccccg ctgtcaaggt gcacgagcac cgcagggctc cccgaatcct
     6241 tgaactggag ggcagtaccg ggggccacct ctgacgtctc ggctatagcc tttccgcctg
     6301 gatcctttgg cttcttcgaa ccgccgccag ctgcctgctc gccgccgccg gcattaccgc
     6361 cgtatccacc gccgtcattc ccgccgtttc caccgccgcc agagccgtta ccggacccgc
     6421 cgccacctcc ggattcggta ccgccgccac cgcaggcggc gagcacggag gcgctggcgg
     6481 cgcccacgcc cagcgcggtc ccgaggcgta tgaaccttcc gcgcgatatc ttctcttcca
     6541 ctatgctccc ttcatcggct acgacatagc ttaatacgca ggcggcgagg ccgcggttct
     6601 ccatccctcc gtcgcaggcg cgcgtatacg tagaatatag gtgccatgga agctcggaga
     6661 ggagcctggc ggtgaacggc atggtgtggg tgcgcgtact ggtcggggcc gtctggctca
     6721 acggcgccat agagaagttg ctgaacccgg agttcccgca gcagttcgcg gccagcctcc
     6781 aggccggggg cttcgtctcg caggcgcctc ccttcttcca ggagttcatg cggagcactg
     6841 tcatcccgaa cgccgagcta ttcgcgcagc tgatgcgcgc cggggagctg gcgctggggg
     6901 tggccctgat cctgggcctc ctcaccaacc ttgccgcatt aggcagcgtt ggcttgagca
     6961 tcgtcatcat cctctcccag ggcggcgtta ggctcggcac gggcctcggg gcgcccgaac
     7021 tcctcaccat caacgtggtg gtggctttgc tctcggtcgt gatcctgctc agcgcggccg
     7081 ccaaagatct ctccctggac gcgctgctgg cgcgacgtag cgcgtggctc tcaccactgc
     7141 tgctcaaccg tcgccaggtc tagtcctgca ggcgctcgag agtaatcgga ctcggcgggc
     7201 gcgaagcgtc acgttttcgc cccggagcac gctcctacag gaaagccggg acgagctcgc
     7261 ccttcaccag gtcggcgtgg ccctcgcgct cgcgcaccac cgcccacctg tctcccttga
     7321 cgaggacctc ggcggggcgg ggacgggagt tgtagttgga ggccatcgag aagccgtagg
     7381 cgccggcgca catgacggct agcaggtcgc cctccctcgc gtcgggcagc tcgcggtcgc
     7441 gggcgaggta gtcgccgctc tcgcacaccg ggccgaccag gtcggcgttg gcgaccgagc
     7501 cgtcctcgct taccgcgcgc acctcgtggt gggcgtcgta gaggctggga cgcaggaggt
     7561 cgttcatccc cgcgtcggct acgatgaagt ccttctcgcc gctcttcttg cggtatatga
     7621 cgcgggtcag gagggcgccg gccccaccgg cgatgtagcg gcccatctcg cacaggatct
     7681 tcgtccccgt cgcctcgagc gtcgggcgga cggcggcgac gagctcttca gggctgggag
     7741 cctcctcgtc cttgtagcgg atgcccaatc caccgcccat gttgaagtat ctgatatcga
     7801 agccgcggtt cttgagctca tctaccagcg tggcactctt ctcgacggac tcggtgaagg
     7861 gggcgagctt ggtgatctgg gatcctatgt gctggtgaac cccgatgagg tcaacgctcc
     7921 tgtactgccc cacccgctcg gcgagcgcga gcgcctcgtc aacggggatg ccgaacttgt
     7981 cgaaagccct tccggtggcg acgtgctcgt gggtcccggc gtccacgtcc gggttgaccc
     8041 tcaacgcgat gcgcgcccgc ttgccgtgcc tactggccag acggtcgagg tgctcgagct
     8101 ccgaggccga ctcgacgttg aacagcaaaa tgcgctctcc aagacccgcc atgagctctc
     8161 tctcggtctt gccgaccccc gcgaagacga ccttcttcgg gtcgaacccg gcgcgcatag
     8221 cgcggtaaag ctcgccgacc gagacgatgt cggcccccgc cccgaaggac gccagggcgc
     8281 gcaggacggc caggttcccg ttcgccttca ccgcgtagca gaccatgtga tccaggcccg
     8341 aaaaagcccc gtccagctcc ctgtacgccc tctccagagc accgtagctg tagacgtaga
     8401 ccggcgtccc cgcgccctct gctatctcgg agagcggcac gtcctcgcaa cggaggacgc
     8461 cggcctcgtg gtggaattcg tccaagggtc tacctccttc tttccccgct tcgcggtccc
     8521 ataagaagtg ctatatttct tgctcttgca agcgaggtag agggaatata ccgcgtgccg
     8581 gaacccggcg cgacaagacc atgaagggcc ggcgcaggct cggccgcacg tccttcccgc
     8641 cactcctgct gaaccccgcg ctggagcgga gcggcgccta ccccctgctc cgccttgagg
     8701 aacgccggcg cgaggttaga gagaggggag tcgagctttt cgacttcggc acgggcgacc
     8761 cgcgcgagcc gacggacccg aagataaaac gggccctcgt agacggcgtc cccgaggtga
     8821 gccagtaccc gagtgctcaa ggcaagagga gcttgcgcga ggccttcgcc ggctggatgg
     8881 agcggcgcaa tggcgtcgcg ctcgaccccg aggcggaggt cctgcccgcg accggctcca
     8941 aagaggcgat cttccacgct cctctagcct tcctgcaccc ctcgcacgag cggcgcggcg
     9001 tcgcctacgg tacgcccggc tatccggtct acgaacgggg cacgctcttc gccggcgggg
     9061 aacctgtgcc gctcaggttg aaagctgggg acgagttctt gctcagggtg gaagagctgg
     9121 acccgcagaa catcagggcg ctctggatca actaccccca caacccgacc ggcgcgacgg
     9181 cgccttacga gtatctggag aaggttgccg cgttctgccg cgcgcacgac atccttctct
     9241 tctccgacga gtgctacaac gacctctact tcagcgatcc tccaccctcg atcctcgaga
     9301 taacccgtga gcgcacgctc gccttctgct ccctgtccaa gcgcagcggc atgaccggct
     9361 accgctcggc gatgatggcc ggagacgcgg agctcatcgc cgctctcaag aaactacgcc
     9421 cctccatcgg cgtcgccacc caatccttcg tgcaggacgc tgcgaccgcc gcctggaacg
     9481 acgacgtgca cgtcgaaaag aggcgccaga tatttgccga gaagcgggag ctcttcaagg
     9541 acttcttcga gcgggcgggg ctccgctaca cgcgcaccga cgcgagcatc tacctgtgga
     9601 ttacggtacc cgaagcgttc ggtggggacg acgaggcgta cgccctgcgc ctgcttgagg
     9661 aaggcatcgt cgtggcaccg ggacgttcgt tcggtcctgg cggcgagggc tacgtcaggg
     9721 ttgctctcgt cccacggctg gaggagtgcc ggaaggcgat agagaagtgg gagggtctga
     9781 tccgccggga cttctcagcc taacgccagc gccgcgaaac cgaccaccat aacggtggcg
     9841 gcgacgagac gccggcggac gtccccttcc gcgagcaggc tcgcgcccat cgcggccccg
     9901 atgaggatgc tgatctccct cgccggagcc acgtagctta caggcgtgaa gacgagcgcc
     9961 gtaaggacca ggatatacga gagcgggctg agcaccgcga cgcctagcgc gtcgatcttg
    10021 tgttctcgcc agatcgccag caccttcccg cgatggcgca cggccagggg ggtgagcaac
    10081 cccgtggtga ccagggtcgt gacccagctc tgcaagagag gcgggatcag cagcgcgctc
    10141 acggcgtgct tgtcccagat ggtgtaggcg g
//
LOCUS       DASTUV010000127         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_165344, whole genome shotgun sequence.
ACCESSION   DASTUV010000127 DASTUV010000000
VERSION     DASTUV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165344"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..>2052
                     /locus_tag="VFT03_02655"
     CDS             280..>2052
                     /locus_tag="VFT03_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4847074.1"
                     /translation="MKVIVARDIVKYHGGDLKVLSGASLSVEAGEKIGLVGRNGAGKT
                     TLLEILAGNSEADSGSVERIGGAKVGVTSQSLYAGERGRISVEQEITSAFEPLMRRER
                     ELEELEARLAEEHSPLLLERYGRLQGEFERDGGYDYRARAASTLSGLGFAPEDWKRPV
                     GSFSGGEQSRIALARLLLEEPDLVLLDEPTNHLDLEAIEWLENFVKGAKSAVLVVSHD
                     RYFLDAVAGSILELEGGKLTRYPGNYSAYVKEKKASEERLARKAKANAERRAQLERFI
                     EKNRAKARKASQARSKQKLLDRMEKVEAPSGANKNMKLDLGDTSRAGRVILEMENVRY
                     AHEDSDEPLLEDLDLVVERGERVGLLGPNGTGKSTVMRLATGELSPQRGFVRLGHNVS
                     PAYQDQQLARLDDNKTVLQEVMDASGIKAPEARDLLGAFLFSGEDVFKKVSSLSGGER
                     NRLSLAEIVVSGANLLLLDEPTNNLDIPAREALEEALLEYRGTMFFISHDRYFLRKLA
                     TRVVDLEGRKLTNYLGGYDYYRSHRRLDARDGKRRPRRRVRPGQSKEESVLASRLVAV
                     EGESDATERRLSRLEKELATSELYA"
BASE COUNT          397 a          661 c          686 g          308 t
ORIGIN      
        1 gaggcagcat cctaggggta cttagaagac gttttggtcc gaggaggagc tgcgcgctct
       61 gcggcggcgc acggagcacc tggcacagct agagcgcaac agggatagcc tgctggagag
      121 ttatgccgat ctcgtgcccg aagcgatcga cgctctggag ccggaggaac gccgaagtgt
      181 gtataaaata atcgggctgg aggcgcgtct ggcgtcggat ggttcgttcg aggttagcgg
      241 agacgtgatg agcttctcca aactagaaac ttcatcggca tgaaggtcat tgttgcacga
      301 gacatcgtca agtaccacgg gggcgacctg aaggtcctct ccggcgcctc gctctcggtc
      361 gaggccgggg agaagatcgg gctggtaggc cgcaacggcg ccggcaagac gacgctgctc
      421 gagatccttg ccggcaactc cgaggccgac tctggctccg tcgagcgcat cggcggcgct
      481 aaagtgggcg tgacctctca gagcctgtac gcgggcgagc gaggcaggat ctccgtggag
      541 caggagatca cctccgcctt cgagcccctg atgcggcgcg agagggagct ggaggagctg
      601 gaggcccggc tcgcggagga acactccccc ttgctcctcg agcgctacgg caggctgcag
      661 ggcgagttcg agcgggacgg aggctatgac tacagggcgc gggcggcctc taccctctcc
      721 ggcctgggct tcgctccgga ggactggaag cgtcctgtgg ggtctttctc gggcggggaa
      781 cagagccgca tagccctcgc ccggctcctg ctcgaggagc ccgacctcgt cctgctcgac
      841 gagccgacga accacctcga cctcgaggcc atcgagtggc tcgagaactt cgtcaagggc
      901 gcgaagtccg ccgtgctcgt cgtctcccac gaccgctact tccttgacgc cgtagctggc
      961 tcgatcctgg agctcgaagg cggtaagctc acccgctacc ccggcaacta cagtgcgtac
     1021 gttaaggaga aaaaggccag cgaagagcga ctcgcccgca aggccaaggc gaacgccgag
     1081 cgcagggcgc agctcgagcg ctttatcgag aagaaccggg cgaaggcgag gaaggccagc
     1141 caggccagga gcaagcagaa gctcctcgac cggatggaga aggtcgaggc cccgagcggc
     1201 gccaacaaga acatgaagct cgacctcggc gacacctcgc gggcggggcg cgtgatcctc
     1261 gagatggaga acgtgcgcta cgctcacgag gactccgacg agcccctcct cgaagatctc
     1321 gacctcgtcg tcgagcgcgg ggagagggtc ggcctcctcg gcccgaacgg cacggggaag
     1381 agcacggtga tgcggctcgc gactggcgag ctatccccgc agcggggctt cgtgcggctc
     1441 ggccacaatg tatctcccgc ctaccaggac cagcagctcg cccgcctcga cgacaataag
     1501 accgtgctgc aagaggtcat ggacgcttcc ggcattaaag cgccagaggc gcgcgacctg
     1561 ctcggcgcct tcctcttctc cggggaggac gtcttcaaga aggtctcctc gctctctggc
     1621 ggcgagagga accggctcag tctagcggag atcgtggtta gcggcgccaa cctgctcttg
     1681 ctcgacgagc cgaccaacaa cctggacatc cccgcccgcg aagccctcga ggaggccctc
     1741 ctcgagtacc gcggcaccat gttcttcatc tcccacgacc gctacttcct gcgaaagctt
     1801 gccacgcgcg tcgtggacct ggaaggcagg aagctcacga actacctcgg cggctacgac
     1861 tactaccgct cgcacaggcg actcgatgcg agagatggca agcgtcgccc ccgccggcgg
     1921 gtccgtccgg gacagagcaa agaagagagc gtgctcgcat cacgcctcgt cgcggtggag
     1981 ggcgagagcg acgccaccga gcgccgcctg agccgcctgg agaaggagct cgccacctcc
     2041 gagctctacg cc
//
LOCUS       DASTUV010000128         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1381726, whole genome shotgun sequence.
ACCESSION   DASTUV010000128 DASTUV010000000
VERSION     DASTUV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381726"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..930)
                     /locus_tag="VFT03_02660"
     CDS             complement(394..930)
                     /locus_tag="VFT03_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEU4847075.1"
                     /translation="MRRTVHGVSVECVRGDITSQEDVAAIVNAANAELRSGGGVAGAI
                     HRAAGPGLAREARPLAPISPGEAVITGAHDLPNRYVIHTLGPVYGRDRPEAELLANCY
                     RNSLALAEEKGLDSVAFPAISTGIFGYPVEEAARVALGTVVEEAERLESVRLIRFVLF
                     GEKDLEVHERVLSETEGR"
     gene            complement(927..2108)
                     /gene="nagA"
                     /locus_tag="VFT03_02665"
     CDS             complement(927..2108)
                     /gene="nagA"
                     /locus_tag="VFT03_02665"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU4847076.1"
                     /translation="MREADSARLVALRGQVVTDYEVWPEGTILFEGGSIVDVSPDDSL
                     LGDAEEVHEHPDSLLLPGFVDLQVNGAFGVDVATEPSRLGELSQALLSTGTTAYLPTI
                     ISSPASLYEQALPELAAAVGGPASGAEVLGVHLEGPFINLDRRGAHAAAHVVAPDPRL
                     LARLLDLAPVRMLTLAPELEGAGELMALALGRGVVVCAGHSDATFEVAYEALDASVAG
                     VTHLFNAMKAMHHREPGLQGAAFTHPRVVCGLIADGLHVHPEMVALAVRMLGPDRVCL
                     VTDAIEAAGMGDGEYRLATRTVYFDGGVPRLGSGAIAGSVLTMDEAFRNVLAFTNCTL
                     PEAARMVSTTPARLVGEGRRKGLITPGYDADVAVLAPDLSVEAVWLGGRVVYDKEESS
                     R"
     gene            complement(2120..>2472)
                     /locus_tag="VFT03_02670"
     CDS             complement(2120..>2472)
                     /locus_tag="VFT03_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847077.1"
                     /translation="AAVGPWMLYLAGPLVGIALGGTWTASRVMLVALSPPEKLGEFFG
                     LFSLASKFSAVFGPALTATMLVVLGSLEATAYRMSIGLLAVIMSLGILLLARVPDARP
                     DTTVEEFAPVAHVK"
BASE COUNT          411 a          837 c          818 g          406 t
ORIGIN      
        1 aaccgccagg atagagaaag ccccatgacg tgtatatttt aaaccacgcg cgcatttcag
       61 cacgctccgg cctgcggcct ccgcttgtca gcatttcagc ctattttcgt tttgggaggc
      121 gcgggctctc ccgagttctt ggcgggatcg gtggttttta agtactggtg taggctcgta
      181 tagaaggacc ggcggccccc ctcccagcca gccaccggca gcccagtatc ctaactaaag
      241 atgccggcgc ctgctcttgg gtgtttcgtt ccggcgtccc agggtatatg tgcattacag
      301 gtccggcagc ccacagagtg agaaatcttt gtgagttccc cctccaaagc agccgggcta
      361 ttaccacgag gccggggttt cgacgccccg gccttaccgt ccttccgtct ccgagaggac
      421 cctctcgtgg acctcgaggt ccttttcgcc gaagagaacg aacctgatga ggcgtacgct
      481 ctccagcctc tccgcctcct ccacaaccgt cccgagcgcc accctcgcgg cctcctctac
      541 gggatagccg aagattccgg tcgagatcgc cgggaaggcc acggagtcta gccccttctc
      601 ctctgcgagc gcgagggagt tgcggtagca gttggccagt agctccgcct cgggacggtc
      661 ccggccgtag actggaccca gggtgtggat gacgtaacgg ttgggcaggt cgtgggcgcc
      721 ggttattacg gcctcaccgg ggcttatcgg agcgagcggg cgcgcctccc gagcaagccc
      781 ggggccggcc gcgcggtgga tcgcgccggc tactcctcct ccgctgcgca gctccgcgtt
      841 cgccgcgttg acgatcgccg ccacgtcctc ctgcgacgtt atgtcacccc gcacgcactc
      901 gacgctcacg ccgtgcaccg ttctcctcac cggctgctct cttccttgtc gtagaccacc
      961 ctgccgccaa gccagacggc ttctaccgag agatcggggg cgagcaccgc cacgtcggcg
     1021 tcgtagcccg gggttatgag ccccttgcgc ctgccctccc cgacgagacg cgcgggggta
     1081 gtcgagacca tccgagcagc ctcggggagg gtgcaattcg tgaacgccag gacgttccgg
     1141 aaagcctcgt ccatggtgag gacactgccc gcgatagccc cgcttcccag cctgggaacg
     1201 ccaccgtcga agtagacggt cctggtagcc aggcggtact cgccatcccc catgcccgcg
     1261 gcctctatgg cgtcggtgac gagacagacg cggtcagggc cgagcatgcg gacggcgagg
     1321 gcgaccatct cggggtggac gtggagtcca tcggcgatga ggccgcagac gaccctcggg
     1381 tgggtgaagg cggctccctg aagaccgggc tcgcggtggt gcatggcttt catggcgttg
     1441 aagaggtgcg tgacgccggc taccgacgcg tcgagcgcct cgtaagcgac ctcgaacgtg
     1501 gcatcggagt gtcccgcaca gacgaccact ccgcgcccca gggcgagcgc catgagctcg
     1561 cccgcgcctt cgagctcggg ggcgagggta agcatgcgta cgggcgcgag gtcgaggagg
     1621 cgagcgagca acctcgggtc cggcgccact acgtgcgcgg cggcgtgcgc gccccttcta
     1681 tccaggttta tgaacgggcc ctcgaggtgt acgcccagca cctccgcgcc ggaggcgggt
     1741 cctccgaccg cggctgcgag ctcgggcagg gcctgctcgt agaggctcgc gggggaggag
     1801 atgatggtag gcaaataagc ggtggtcccc gtcgagagca gagcctggga gagctccccg
     1861 aggcgggagg gctccgtcgc cacgtccacg ccgaaggcgc cgttgacctg caagtccacg
     1921 aagccgggca ggagcaggga gtcagggtgc tcgtgcacct cctcggcgtc gcccaggagc
     1981 gagtcgtcag ggctcacgtc gacgatagac ccgccctcga agaggatggt cccttccggc
     2041 catacctcgt agtccgtgac cacctgcccc cggagcgcca cgagcctggc gctatcggct
     2101 tctctcacgc tttaccccct cacttgacgt gcgccaccgg cgcgaactct tccacggtag
     2161 tatccgggcg ggcgtcgggg acgcgcgcga gcaggaggat gcccagagac atgatcacgg
     2221 cgagcaatcc tatggacatg cggtacgccg tcgcttcgag gctcccgaga acgactagca
     2281 tggtcgccgt gagcgctggt ccaaatacgg cggagaactt gctcgcgagc gagaagagcc
     2341 cgaagaactc gccgagcttc tcgggcgggg agagcgcgac gagcatgacc cggctggcgg
     2401 tccacgtccc cccgagcgcg atccccacca gaggccccgc gaggtagagc atccaggggc
     2461 cgaccgccgc cg
//
LOCUS       DASTUV010000129         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1320311, whole genome shotgun sequence.
ACCESSION   DASTUV010000129 DASTUV010000000
VERSION     DASTUV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320311"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VFT03_02675"
     CDS             <1..1187
                     /locus_tag="VFT03_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4847078.1"
                     /translation="LTELGIPHPRFGTASTAQEAREVARELGYPVVVRPSYVLGGRRM
                     EIVYDNDNLDLYLRTSADTSADHPTLIDKFMEDYVEVDVDAVSDGEDVYVGGIMEHVE
                     EAGVHSGDSSCVTPPITIHRALLDKIEDYTRRIALAVGVVGLMNVQFVVRGEDVLVIE
                     CNPRASRTVPFISKATGVPLAKVATRVLLGEKLRDMGLHRTTNGHFSVKAPVFPFDRF
                     ADVDPLLGPEMRSTGEAMGIDRTFGGAFAKALTAAGQSLPIAGRVYVSVANRDKRAVV
                     LIARAFADLGFEIAASEGTAEVLKNNGLPVAVVSKIGELGGDVLGMIEAGGVDLILNT
                     PWGRGARTDGYLIRRKALMHGVPCITTLAAAAAAVQGIEARIRGGARRVNSLQGLYAT
                     RA"
     gene            1184..1912
                     /locus_tag="VFT03_02680"
     CDS             1184..1912
                     /locus_tag="VFT03_02680"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU4847079.1"
                     /translation="MRFFSVHVEARTESGNYTVLRYRWPGLVEPGQFVMARAAGYPAT
                     FDPFLARPFSFYDHDGEWASLLFEVRGRGTSLLDRAAKIEVSAPLGRGFRVETSGMRA
                     ALLGGGVGVAPLKILGRRLDELGVRHDVFLGFADGATAAAASDFPGAGVATIDGSVGT
                     HGTVLDAVPDLGSYGAIYACGPNPMLAAVKRAVGEGKLCQLSVEERMGCGNGSCNGCV
                     VPVLGGYVRSCVEGPVFSAEVLAW"
     gene            1906..2811
                     /locus_tag="VFT03_02685"
     CDS             1906..2811
                     /locus_tag="VFT03_02685"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU4847080.1"
                     /translation="MVVRERMAGVRLEVELCGIPLKTPLIPASGTLAKEALGEVEGVY
                     GAILPKTTTPSPRAGNPPPRVAETPAGMVNSIGLQNPGVRGFLRDLDAFDLGVPLFVS
                     AAGDTVEEFGALCERLGADERIAAIELNLSCPNVECGGLTFCATPAAVEEVVAACRRT
                     TSKPLFAKLTFEGVVENALAAEAAGADALTVMNTIPALTLDAHRQSVLVRGGLSGPAI
                     KPVALRAVYDVSRAVRVPVLGSGGVASGLDVAEFMLAGATVVQVGTASFVRDPREILE
                     EFASYLKRVGLDAADLKGALRENSR"
     gene            2852..3178
                     /locus_tag="VFT03_02690"
     CDS             2852..3178
                     /locus_tag="VFT03_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847081.1"
                     /translation="MLRRAPDRTTQERRSALEEANRVRFARAATKRLLKSGELGIYQL
                     LMDPSEELKGAKVEEMLLAVRGMGRIKVTRIMREAGVSRAKTLVGLTHGQRDRLIRAL
                     GCEEDA"
BASE COUNT          541 a          983 c         1177 g          534 t
ORIGIN      
        1 tcctgaccga gctcggcatc ccgcaccccc ggttcgggac cgcctcgacc gcccaggagg
       61 cgcgggaggt cgcccgcgag ctgggctacc ccgtggtggt caggccctcc tacgtactgg
      121 gaggcagacg gatggaaatc gtctacgata acgacaacct cgacctatat ctcaggacga
      181 gcgcggacac cagtgcggat catccaaccc tgatcgacaa gttcatggag gactacgtcg
      241 aggttgacgt ggatgccgtc tcggacgggg aggacgtcta cgttggcggg atcatggagc
      301 acgtagagga ggcgggggtg cactccgggg actcgtcgtg tgtcacgccg cctataacca
      361 ttcaccgggc gctgctcgac aagatcgagg attacacgag gcggatagcc cttgccgtcg
      421 gagtagttgg cctgatgaac gtgcagttcg tggtgcgtgg ggaggacgtg ctcgtcatcg
      481 agtgcaaccc ccgcgcctcg cgcaccgtgc cgttcatctc caaggcgacc ggggtgccgc
      541 tcgccaaggt cgctacacgt gtcctgcttg gcgagaagct gcgtgacatg gggctccacc
      601 gcaccacgaa cggccacttc tccgtcaagg cgccggtgtt tccgttcgac cgcttcgccg
      661 acgtggaccc gctgctcgga cccgagatgc gctcgacggg cgaggcaatg gggatagaca
      721 ggacatttgg aggcgcgttc gccaaggcgc ttacggcggc tgggcagtcg ctgccgatcg
      781 ctgggcgagt atacgtctcg gtggccaaca gggacaagcg ggcggtggtg ctaatcgcgc
      841 gggcgttcgc cgacctcggg ttcgagatcg ccgcgagcga aggcacggcc gaggtgctca
      901 agaacaacgg gctccctgta gcggtggtgt ccaagatcgg ggagctgggc ggggacgtgc
      961 tcggcatgat cgaggcaggc ggggtggacc tgatcctgaa caccccctgg ggccgagggg
     1021 cgcgcacgga cgggtacctg atccggcgca aagccctcat gcacggggtc ccctgcataa
     1081 ccactctagc cgccgccgct gccgccgtac aggggataga agccaggatc cggggcggcg
     1141 cccgccgcgt caactccctg cagggcctct acgccacgag agcgtgaggt ttttcagcgt
     1201 acacgtcgag gcgcgcacag agtctggcaa ttacacagtg ctccgctacc gctggcccgg
     1261 actggtggag ccgggccagt ttgtcatggc gcgggctgcc ggataccctg ccaccttcga
     1321 cccgttcctg gcgcggcctt tctccttcta cgaccacgac ggcgagtggg cgagcctgct
     1381 attcgaagtg cgaggacgag gaacttcgct tctcgatcgg gccgcgaaga tcgaggtcag
     1441 cgctcctctg ggccgcggat tccgggtaga gacttccggc atgagggcag cgctgctcgg
     1501 cggcggtgtt ggggtcgcgc cgctcaagat cctgggccgg cgactggacg agctcggcgt
     1561 gaggcacgac gtatttctgg gcttcgccga cggcgccacg gcggctgccg caagtgattt
     1621 ccccggcgcg ggcgtggcga cgattgacgg gtcggtggga acccacggca cggtgctcga
     1681 tgcggtcccc gacctcggga gctacggggc aatctatgcc tgcggcccca acccgatgct
     1741 cgccgccgtg aagcgggccg tcggcgaggg gaagctctgc cagctctcgg tggaggagag
     1801 gatggggtgt ggcaacgggt cgtgcaatgg gtgcgtggtc cctgtgcttg gcggctacgt
     1861 caggtcttgc gtcgaggggc cggtcttcag cgcggaggtc ctggcgtggt agtgcgcgag
     1921 cggatggcag gggtcaggct cgaggtcgag ctgtgcggca tcccgctgaa gacgcccctg
     1981 ataccggcct ccggtacgct cgcgaaggag gcgctcggcg aggtcgaggg agtgtacggg
     2041 gcgatactgc caaagaccac taccccgtcg ccgagggccg gcaatccgcc gccgcgggtg
     2101 gcggagacgc cagcagggat ggtcaactcc atagggctcc agaacccggg tgtccgaggg
     2161 ttcctgcgag acctcgacgc cttcgacctc ggcgtcccgc tcttcgtctc ggctgcggga
     2221 gacacggtag aggagtttgg cgcgctctgc gagcgcctcg gcgctgacga gaggatcgcg
     2281 gccatagagc tgaacctctc gtgccccaac gtggagtgcg gcgggcttac cttctgcgca
     2341 acccccgcag cggtcgagga agtggtcgcc gcttgccggc ggaccacgag caagcctctg
     2401 ttcgccaagc tcaccttcga gggtgtagtc gaaaacgcgc tcgcagctga ggcggcgggg
     2461 gccgacgcgc tgacagtcat gaacaccatc cctgcgctca ccctggatgc ccaccgccag
     2521 agcgtgctgg tgcgaggcgg actctccggc ccggccataa agcccgtggc gctgcgggcc
     2581 gtctacgacg tctcgcgcgc cgtacgggtg cctgttctcg ggagcggcgg cgtcgcgagc
     2641 gggttggacg ttgcggaatt tatgctcgcc ggggccacag tcgtgcaggt cggcacggcg
     2701 agcttcgtgc gggatccaag agagatactc gaggagttcg cgagttacct gaaaagggtg
     2761 gggctcgatg ccgcggatct gaagggcgct ttgcgggaga attcgcggta aaacggcggg
     2821 ttgaaggacc cggtggtata ctagcgggtc gatgttgagg agggcaccag acaggacgac
     2881 ccaggaacgg cgcagcgcgc tcgaggaggc aaaccgggtg cgtttcgcca gggctgctac
     2941 caagcggctg ctcaagagcg gggagctagg tatctaccag ttgctcatgg acccctccga
     3001 ggagctcaag ggcgccaagg tggaggagat gctcctcgcc gtgcgtggga tggggcgcat
     3061 aaaggtgacg cgcatcatga gggaagccgg ggttagccgc gccaagaccc tcgttggact
     3121 gactcacggc cagcgggacc gtttgatcag ggctctgggg tgcgaagagg acgcctgatc
     3181 gtcgtctccg gtccgtccgg agccggaaag gacaccttga taggggatgc cctcg
//
LOCUS       DASTUV010000130         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1672095, whole genome shotgun sequence.
ACCESSION   DASTUV010000130 DASTUV010000000
VERSION     DASTUV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672095"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFT03_02695"
     CDS             <1..754
                     /locus_tag="VFT03_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4847082.1"
                     /translation="VERGGGSMWLGNREWQIEAGDVFLIAPGEVVGADDDASGLHEPE
                     GWAVFFQPEVLGSEAPGAFLSWRAHPLLFPFVRGAAGGAQRLKVPPEERPTFSEHLWA
                     LELELRHRQDGYREAVLAYLTLVLVGVGRLAVDVVGDLRLKDEPLLAEVFGFIEDRYH
                     EPISLRNVARAVSLSPGHLTTVVRRKTGRTAQEWIAERRMAEARRLLVESDLSVEEVG
                     RRVGYSESSYFVRSFRRAHGATPLGWRRAGRP"
     gene            917..1957
                     /gene="sppA"
                     /locus_tag="VFT03_02700"
     CDS             917..1957
                     /gene="sppA"
                     /locus_tag="VFT03_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEU4847083.1"
                     /translation="MEPESNQGTQAENGGTGGRRRRRRWPFVVGGLVALGLLVLSVGL
                     VVAILAVSAAGPAASAPAYEEEYVSGEGPDKVAVVPVVGTIASADSTVPGTQPPATSE
                     GLADALRQAAEDQSVAAVVLEVNSPGGGVTASDEMHQSILDFKKSSGKPVVVSMGDTA
                     ASGAYYISTAADRIVANETTLTGSLGVIFSLTNISELADKYGVKEEVVKSGKFKDMGS
                     SFRDLTPEEREILQSIVDESYDEFVEVIVKGRGLPEERVREIADGRVYSGEQAKELGL
                     VDSFGGLDEAAEVSEDLAGVDEATVVRYVQPESFTDALLSSFAPQKPEALQIAEEAGL
                     TLEPKPYYLYLP"
     gene            complement(2098..>2444)
                     /locus_tag="VFT03_02705"
     CDS             complement(2098..>2444)
                     /locus_tag="VFT03_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEU4847084.1"
                     /translation="WHIGTPYRPSPPGPCRAYQSEDCACTTKLVFKKFGERLPDNPVR
                     QWRKGKKIRKSNVRPGDLVFFKEAGRNRPITHVGIYSGHGNLIHASNFFGKVVESKMK
                     YMRGYIGAKRIR"
BASE COUNT          424 a          770 c          808 g          442 t
ORIGIN      
        1 cgtcgaacgg ggcggcggct cgatgtggtt gggaaaccgg gagtggcaaa tagaggctgg
       61 cgacgtgttc ctcatcgccc ctggcgaggt ggtgggagcg gacgatgatg cgagcggcct
      121 ccatgagccc gagggctggg ccgtcttctt ccagcccgag gtcctcgggt ctgaggcccc
      181 cggcgccttc ctctcctggc gcgcccaccc cctgctcttc cccttcgtta ggggcgcggc
      241 tggaggagcc caacgcctta aggtgcctcc cgaagagagg cccactttct ccgagcacct
      301 ctgggccctc gagctcgagc tccgccacag gcaagacggc tacagggagg cagtcctcgc
      361 ctacttgacg ctcgttctag taggtgtggg gcggctggcg gtggacgtgg tgggcgatct
      421 caggctcaag gacgagcccc tgctcgccga ggtcttcggc tttatagagg atcgctacca
      481 cgaacccatc tctctgagga acgtggcgcg agcggtgagc ctctcgccag ggcacctcac
      541 cacggtcgtc aggcgcaaga ccggacgtac agcgcaggag tggatcgccg agcggcgcat
      601 ggcggaggcg cgcaggttgc tggtcgagag cgacctttcg gttgaggagg ttggccggag
      661 ggttggttac agcgagtcga gctactttgt gaggtccttc aggcgcgccc acggcgcgac
      721 cccgcttggc tggcgccgcg ccggccgccc ataagccctc ggggtggata gcgcagtcgg
      781 gattgttgcc gtccttcttg gcgggtggtg cgccgtgtcg tgcgccggtt gccctggcct
      841 tataatcctc ctagccgcgg acctcgtagg ggctccggcg ccgcgatagg acgtacctct
      901 aggaaaggcg aagctcttgg aaccagagag caatcagggt acacaggcgg agaacggcgg
      961 cacagggggc cgcaggagac gccgcaggtg gcctttcgta gtcggcgggc tggttgctct
     1021 cgggctgctg gtcctgagcg tcggcctcgt cgtggccatc ctcgccgtga gcgcggcagg
     1081 gcctgctgcg tcggccccgg cctacgagga ggagtacgtc tcgggggagg gtccagacaa
     1141 ggtagctgtt gtgcccgtgg tgggcaccat cgcctccgcc gacagcaccg tgccgggcac
     1201 ccagcccccc gccacctccg aggggctcgc cgacgccctc aggcaggcgg cggaagatca
     1261 gagcgtcgcg gcggtcgtgc tcgaggtcaa ctctcccggc gggggagtca cagcgagcga
     1321 cgagatgcac cagagcatcc tcgacttcaa gaagtcctca ggcaagccag tcgtcgtctc
     1381 catgggcgac acggcggcct cgggcgccta ctacatctcc accgcagcag acaggatagt
     1441 cgccaacgag accaccctca cgggctctct gggcgtgatc ttctcgctca ccaacatctc
     1501 cgagctcgcc gataagtacg gtgtcaagga ggaggtggtc aagagcggca agttcaagga
     1561 catgggttcc tccttcaggg acctcacgcc cgaggagcgc gagatcttac agtccatagt
     1621 ggacgagtca tacgacgagt tcgtcgaggt gatagtaaag ggccgcggcc tccccgagga
     1681 gagggtgcgc gagatcgccg acggccgcgt ctactccggc gagcaggcca aagagctcgg
     1741 cctcgtcgac tccttcggcg ggctcgacga ggctgccgag gtctccgagg acctcgcggg
     1801 cgtagacgag gcgaccgtcg tccgctacgt gcagcccgag agcttcaccg acgcgctcct
     1861 gtccagcttc gccccgcaga aaccggaggc cttacagata gccgaggaag ccggacttac
     1921 cctcgaaccc aagccttact acctctacct gccctgagcc tagagaaggc cttctctacg
     1981 aacagtcgat agtcacgact cactcggcga gcgatcgacg ctgcgagctc taaagcaagt
     2041 aaagaaagga gggtcccttg cgggaccccc atcagcacta cctcgtggcg tctatctcta
     2101 tcgtatcctc ttcgcgccaa tgtaaccgcg catatacttc attttgctct caactacctt
     2161 gccgaaaaag ttggaggcgt ggatgagatt accatggcct gagtatatcc ccacgtgggt
     2221 tatgggcctg ttccttcctg cctccttgaa gaagacaagg tcgccggggc gaacattgga
     2281 cttcctgatc ttcttgccct tccgccattg gcgaaccggg ttgtcgggca gtcgctcgcc
     2341 gaatttcttg aagacgagct tcgtggtgca ggcgcagtct tctgattggt aagccctgca
     2401 aggccccggt ggcgatggcc ggtagggggt gcctatgtgc cagc
//
LOCUS       DASTUV010000131         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1669619, whole genome shotgun sequence.
ACCESSION   DASTUV010000131 DASTUV010000000
VERSION     DASTUV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669619"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..1139)
                     /locus_tag="VFT03_02710"
     CDS             complement(384..1139)
                     /locus_tag="VFT03_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847085.1"
                     /translation="MAFETCTLKDRPELRDQVGELAREGWPTFLLHSDITHWDTLFDE
                     FAELQILLCEPAGSVVAVGHTIPFVWDGSPDTLPPTMAGLMDRGVRDRRNSETPNALS
                     ALAALVRASHQRRGLSAEILRCMRSLAEERDMHSLVAPVRPTLKSAYPLTPFERYVGW
                     KRDDGAPFDPWLRVHHRLGAEILNVMPRSLFVTGTVSEWEEWTVMRFPESRPYVVPGA
                     LQPVLMDLVRDEGRYEDPNVWLRHPITDAELAS"
     gene            complement(1141..1428)
                     /locus_tag="VFT03_02715"
     CDS             complement(1141..1428)
                     /locus_tag="VFT03_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4847086.1"
                     /translation="MGGTFGDLWGGWLELTLLWVAEPLRGQGYGARLLLAAEEEARTQ
                     GCRGVFLSTFGFQTRTYYDRFGYEVFGELPNYPEGHSLYFMKKMLRDPEPN"
     gene            complement(1706..>2654)
                     /locus_tag="VFT03_02720"
     CDS             complement(1706..>2654)
                     /locus_tag="VFT03_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU4847087.1"
                     /translation="EPSTGETVVRLHAAALNRRDVFVTQGRYPGAKPDALPVILGSDG
                     SGEVVARGDGAAGPDEGTEVVINPALYWGDDPRKPGKEYRILGLPDDGTFAQHVKVPS
                     ENVFPKPSHLSHEEAAAIPLGALTAYRALVTRGGIEKGETVVVPAIGSGVATFVVQMA
                     AALGARLFVTSGEDEKIEKAKELGAEGGVNYNSEDWSRELKSMTGGVDLAVDHVGGEA
                     FDAMVSLARPGSRIVTFGATAGPKVTVVMPRIFLKHLTILGTAMGTNEEFGAMLDLFA
                     EHGLRPTINETFPLQETAAAMEYMEEGSGIGKIVLDIPA"
BASE COUNT          413 a          864 c          883 g          494 t
ORIGIN      
        1 actggctctc tggtctcttc gggggttctt ctgcgggtcg cgtctctcct cggcacgcgg
       61 caatggcgat gggatgagcc gcggtaaaca ggcctccccg tagcacgagc cgccatgtaa
      121 ggcgggtggt aggataccgc agcgtccggc tgtgtgtcgg ctcaagcgcg cctccgtgtc
      181 tataagcgcc atgacgtcaa tcacccggtg aggcgcgaca gtatccggaa ggtgtggact
      241 acccggcgag caaggaggag gtggcctctg ccgagggcaa aggcgcgccg gaggcgctgg
      301 tgggcatgat ccaatcccct ggcccacgag ctctccggct ccgaagtggt cgtgagcgag
      361 ctgcgggcct tcccccagtg gggctagctc gctagctctg cgtccgttat cggatgccgc
      421 agccagacgt tagggtcttc gtagcggcct tcgtcgcgta ccagatccat gagcacgggt
      481 tgtagcgcgc cggggacgac gtagggtcgg ctctcaggga acctcatcac cgtccactct
      541 tcccattcgg agaccgtgcc ggtgacgaac agggatctcg gcatgacgtt gaggatctcg
      601 gcgccgagcc tgtggtggac gcggagccag gggtcgaaag gcgcgccgtc gtccctcttc
      661 cacccgacgt agcgctcgaa gggcgtgagc gggtaggcgc tcttcagggt ggggcgtacc
      721 ggggcgacga gcgagtgcat atcgcgctct tccgccagag agcgcatgca gcgcaggatc
      781 tccgcgctca ggccgcgcct ctggtgggag gctctcacga gggcagcgag ggccgagagc
      841 gcgttcggag tctcgctgtt ccgacggtcg cgaaccccgc gatccatcaa cccggccatc
      901 gtgggcggca gggtgtcagg actcccgtcc cagacgaagg ggatggtgtg tccgacagca
      961 acgacagagc ctgcgggctc gcatagcagg atctggagct ccgcgaactc atcgaagagg
     1021 gtatcccagt gggttatgtc actgtgcagc aagaacgttg gccagccttc cctggccagc
     1081 tcgcctacct gatcgcggag ctccggccta tccttcaggg tacaggtctc gaaggccatc
     1141 ctagttcggc tccgggtctc gcaacatctt cttcatgaag tacaggctgt ggccttcagg
     1201 gtaattcggg agttccccaa agacctcgta gccgaagcga tcgtagtagg tccgcgtctg
     1261 gaagccgaag gtgctcagga acacgccccg gcatccctgc gtccttgcct cttcttcggc
     1321 ggcaaggaga agcctcgcgc cgtatccctg gccgcgcagc ggctccgcca cccagaggag
     1381 cgtgagctcg agccagcctc cccagaggtc tccaaacgta ccacccaaga tcgcggcgcg
     1441 ctcgtccttg gggagtccct gaggaagatg gcgagtgggc tgtaataggt atcgtgggtg
     1501 actgcgacgt tgtggcgggc gatagcctcc ctgacaaagg tgatctcctc ctggctggcg
     1561 tgtggtttcg aggtgatcct gaggttccgc acgagcctct cctgtcgagg ttgtcggcgg
     1621 ggcgcatcct accccagacc ggcttcgcgg gcgggccatg acgtgtcacg gcccgcgagg
     1681 gccctcgttt cctgcttcgt gagtactacg cgggtatgtc gaggactatc ttcccgatac
     1741 ccgacccttc ttccatgtac tccatcgcgg cagcggtttc ctgcagcggg aaggtctcgt
     1801 tgatggtggg ccgcaggccg tgctcggcga agaggtcgag catcgccccg aactcctcgt
     1861 tcgttcccat cgctgtgccc aggatcgtga ggtgcttcag gaagatcctg ggcataacaa
     1921 ccgtgacctt aggccccgcc gtcgccccga aggttacgat gcggctgcct ggcctggcga
     1981 gcgagaccat agcgtcgaag gcttcgccgc cgacgtgatc caccgccagg tccacgcctc
     2041 cggtcatgct cttcagctcc ctggaccagt cctccgagtt gtagttgacg ccgccctctg
     2101 cgccgagctc cttcgccttc tcgatcttct cgtcctcgcc cgaggtgacg aatagccgcg
     2161 ctcctaaagc ggcggccatc tgcaccacga acgttgccac cccgctcccg atggccggca
     2221 ctacgaccgt ctcgcccttc tctatcccgc cgcgggtgac gagcgccctg taagcggtga
     2281 gggcgcccag gggtatcgcg gcggcctcct cgtgcgagag gtgagagggc ttcgggaaga
     2341 cgttctccga gggcaccttg acgtgctggg cgaacgtgcc gtcgtcgggg agcccgagga
     2401 tgcggtactc cttgccgggc ttccgcgggt cgtcgcccca gtagagcgcg gggttgatga
     2461 ccacctccgt cccctcgtct ggaccggctg cgccatcgcc gcgggcgact acctcgcccg
     2521 agccgtccga gcccagtatg accggcagcg catcgggctt cgcgcccggg taccggccct
     2581 gggttacgaa gacgtcgcgg cggttgaggg ccgcggcgtg gagcctgacg accgtctcgc
     2641 cggtactggg ttcc
//
LOCUS       DASTUV010000132         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_379809, whole genome shotgun sequence.
ACCESSION   DASTUV010000132 DASTUV010000000
VERSION     DASTUV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379809"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFT03_02725"
     CDS             complement(<1..960)
                     /locus_tag="VFT03_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU4847088.1"
                     /translation="MIGATGGAAEDLAAEMLAVGLFKGQAPEGLGDAAAKVVSSGDFT
                     GKEGETALLYGQDGLGAARLLLLGLGERERFDLERLRRASATAAKRARNLKLHDAAFS
                     LPLVPGAGVREAAQAAAEGASLGLYHFDRHKSGTERHELETFELVSDEKDLGQTIEGA
                     EIGQKAAWGASLARDLANEPSNVATPEYLAQKAREIAERHDMEVTVLDRSGIEEEGLT
                     GLATVGRSAANDPRFIVLEHRKGGDAAPIVLVGKAVTFDSGGISIKPSAGMENMKFDM
                     SGGAAVLGAMEAVGDLDLPLNVVALLPATENLPGGDAFKPGDVL"
     gene            1032..1850
                     /locus_tag="VFT03_02730"
     CDS             1032..1850
                     /locus_tag="VFT03_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU4847089.1"
                     /translation="MVSVVELREYLDFAAQTAYEAGRLTLGYFQTDTLRTEIKPDDTP
                     VTAADREAEELIRARIAEEYPGHAVVGEEYGVEGDASHRWIVDPIDGTKAFVRGVPLY
                     GVLIGLEIEGTCEVGAAYFPPLDEMIYAATGAGCYRNGRRSRVSSTDNLEDAILSFTD
                     AASFEEHGREEAFRRLCRTAGGSRGWSDAYGHALVATGRVELMLDPVMNPWDCAPFLP
                     ILREAGGYFGDWSGNETIYAGEALSTTRTLLPEVLHLIRGQTDEGRGIFPAPQT"
     gene            complement(1863..3194)
                     /locus_tag="VFT03_02735"
     CDS             complement(1863..3194)
                     /locus_tag="VFT03_02735"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU4847090.1"
                     /translation="MDTTSKQTVLQEAKDSNVKFIRLWFTDILGTLKSFAITVDELED
                     ALDGGMGFDGSSITGYQDIEESDMIAMPDPSTFKTIPWSPQEAPTARMICDVRTPDGD
                     PYVGDPRHVLRRALERASEMGFDNFFCGPELEYFYFRNSNGTEPLDYGGYFDLTTLDA
                     ATSLRRETVMALQELGIDVEYSHHEVGISQHEIDLRFDDALTMADKVQTYKTVVKEIA
                     TKHGVYATFMPKPIRDQNGSGMHTHQSLFSGESNAFFDSNDQYFLSDTAKQFIAGQLR
                     HAREISIIFAQFVNSYKRLVPGYEAPVYIAWSRRNRSALVRVPLYHPGKEKATRAELR
                     CPDPAANIYLCFAALLHAGLEGIEKGYELPEPMERNLYHLSHEEREKLGIQSLPADLG
                     EAIFEAENSELLYKALGEHVFTRLLELKRAEFEDYRVQVTPYEIQKYLPIL"
     gene            3408..4274
                     /locus_tag="VFT03_02740"
     CDS             3408..4274
                     /locus_tag="VFT03_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847091.1"
                     /translation="MTDKPEDRPEERRRTGPLGDDADSGTTRREPPGDEEPTRQVPAD
                     RPAEGEQDTARETQPVPERPAGEPAGETAGETEDKETRVIRTPPAYGTQADASPYPRG
                     YFEAAEEREDRLRDMYGGVDWLASFIGFIFALVAGSFFSLVAGVVVAPLGVSMDLAGS
                     EIGPAAITGLVLVAILVFLTYFFGGYVAGRLARFDGGRNGAMLLLWTGVTVLVLALIN
                     AVLSGFLPEGISENIDSLIQNFLFSTIGGLSGLGIAGVLIFVGALLVALVGGFLGGRL
                     GSRYHAEIDRTT"
BASE COUNT          735 a         1406 c         1458 g          822 t
ORIGIN      
        1 caggacgtcg ccgggtttga atgcgtcacc gccgggcagg ttctcggttg ccggcaagag
       61 ggcgacgacg ttgagcggga gatccaggtc ccccacggcc tccatcgctc ccaggacagc
      121 agcccccccg ctcatgtcga acttcatatt ctccatcccg gccgacggct ttatggagat
      181 gccgcccgaa tcgaaggtca cggccttgcc gacgaggacg ataggcgcgg cgtccccacc
      241 cttcctgtgc tccagaacga taaagcgcgg atcgttggcg gccgacctac ctacggtcgc
      301 gagccccgtg aggccctcct cctcgatgcc ggacctgtcg aggaccgtca cctccatgtc
      361 atgcctctcc gctatctcac gcgccttctg cgcgagatac tcgggggtgg cgacgttgga
      421 gggctcgttg gccaggtccc gggcgagaga ggctccccaa gcggccttct ggccgatctc
      481 ggcgccctcg atggtctgcc cgaggtcctt ctcgtccgag acgagttcga aagtctcgag
      541 ctcgtgacgc tccgtgccgc tcttgtgccg gtcgaaatga tagaggccga ggctcgcgcc
      601 ctcggccgcc gcctgtgctg cctctcgtac ccctgcgccc ggcactagcg gcagggagaa
      661 agccgcatcg tgcagcttca ggttccgggc gcgtttggcc gcggtggctg aggcccgccg
      721 cagccgctcc aggtcgaaac gctccctttc tccaaggccg aggagcagga gccgggccgc
      781 gccgagccca tcctgcccgt acagcagtgc cgtctcaccc tccttccctg tgaagtcccc
      841 gctcgagacc acctttgccg cggcgtcgcc cagcccttcc ggggcctgac ctttgaagag
      901 acctaccgcg agcatctcgg cggcgagatc ctcagccgcg ccacctgtcg cgccgatcac
      961 gaccaagcct ggaccacctc tctgccgaag gacgaacggc gtagattata gggcaagctc
     1021 gtcgcggtgc tatggtgtcg gttgtggagc tgcgagaata tctggacttc gccgcgcaga
     1081 ccgcctacga ggccggtcgc ctcaccctcg gctacttcca gacggatacg ctgcggacgg
     1141 aaatcaagcc cgacgacacg ccggtgacgg ctgccgacag ggaggcagag gagctcatcc
     1201 gcgccaggat cgcggaggag tacccgggac atgctgtcgt gggagaagag tacggggtag
     1261 agggggacgc gagtcaccgc tggatcgttg accctatcga cgggacgaag gcgttcgtcc
     1321 gaggtgtccc gttgtacggg gtcctgatcg ggcttgagat agagggcacc tgcgaggtgg
     1381 gcgccgccta cttcccaccc ctcgacgaga tgatctatgc cgcgactggt gcgggctgct
     1441 accgcaacgg ccgccgctct cgggtgtcga gcacggataa cctggaagat gccatcctct
     1501 ctttcaccga cgcggcgagc ttcgaggagc atggccgcga agaagccttc cggcgcctgt
     1561 gtcggacagc cggtggtagc cgcggctggt ctgacgccta cggccacgcc ctcgtcgcca
     1621 cgggcagggt agagctcatg ctagatcccg tcatgaaccc ctgggactgc gcgcctttcc
     1681 tgcccatcct gcgcgaggcc ggcggctact tcggcgactg gtcaggcaac gagacgatct
     1741 acgccggcga agccctgagc accacgagga cgctattgcc cgaagtgctc catctcatta
     1801 gagggcaaac agacgagggg cgcgggatct ttcccgcgcc ccaaacctga tactgaagaa
     1861 gcctacaaaa tcggcagata cttctgtatc tcgtaggggg tcacctggac cctgtagtcc
     1921 tcgaactccg cccgcttgag ttcgaggagt ctggtgaaga cgtgctcgcc gagggccttg
     1981 tacaggagct ccgagttctc ggcctcgaag atggcctcgc cgaggtcggc tggcaggctc
     2041 tggataccga gcttctccct ctcttcgtgg gagaggtggt agaggttgcg ctccatcggc
     2101 tcggggagct catagccctt ctcgatcccc tcgaggcccg cgtgcaggag cgctgcgaag
     2161 cacaagtaga tgttcgcggc gggatcgggg cagcggagct ccgctctggt agccttctcc
     2221 ttgcccggat ggtagagcgg gacccgtacc agcgccgagc ggttgcggcg gctccaggcg
     2281 atgtagacag gggcttcata gccagggacc aggcgcttgt aggagttcac gaactgggca
     2341 aagatgatgc tgatctcccg cgcgtgcctc agctggcccg cgatgaactg cttcgccgtg
     2401 tctgagagga aatactggtc gttcgagtcg aagaacgcat tgctctcgcc cgagaagagg
     2461 ctctggtggg tgtgcatacc cgaaccgttc tggtcccgga tcggctttgg catgaaggtg
     2521 gcgtatacac cgtgcttcgt ggcgatctcc ttgacgaccg tcttgtaggt ctgcaccttg
     2581 tcggccatgg tcagggcgtc gtcgaagcgc aggtctatct cgtgctggct gatgccgacc
     2641 tcgtggtggg agtactcgac gtcgataccc agctcctgca gcgccatcac cgtctcgcgc
     2701 ctgagcgacg tggccgcgtc gagcgtcgtg aggtcgaagt aaccgccgta gtcgagcggc
     2761 tcggtgccgt tggagttcct gaagtagaaa tactcgagct cggggccgca gaagaagttg
     2821 tcgaagccca tctcgctcgc gcgctccagc gccctgcgca ggacgtggcg aggatcgccg
     2881 acgtagggat cgccgtctgg cgtcctgacg tcgcagatca tgcgcgcggt cggggcctcc
     2941 tgcgggctcc acgggatcgt cttgaaggtc gaggggtcgg gcatcgcgat catgtccgac
     3001 tcctcgatgt cctggtagcc cgtgatcgag gacccgtcga agcccatccc gccgtccagc
     3061 gcgtcctcta gctcgtctac cgtgatggcg aagctcttga gcgtcccgag gatgtcggtg
     3121 aaccacagcc ggatgaactt gacgttgctg tcctttgcct cctgcaacac tgtctgcttg
     3181 ctagtcgtgt ccaagctacc tcctctaact ctcgctttgc gcaccttcac ggcacgggtt
     3241 atcctgacga cacgcgccat gatatgaacg cgcacgccgg ttggatatgg cccgccgtac
     3301 aagccaggca ccggcgtttt ggcttattat gggccggatg tacaaagagt acgtctggtg
     3361 taacgaagcg tcgcccggtg cgtggtggac gcgctaaagg gggaaagatg actgacaagc
     3421 cggaggatcg tcccgaggaa cggcgcagga ccggacctct tggggacgac gctgattccg
     3481 ggacgacccg aagggagccg cccggcgacg aagagcccac gcgccaggtg cccgcggacc
     3541 gacctgccga gggtgagcag gatactgcgc gggagacgca gccggtgccc gagcggccgg
     3601 caggcgagcc ggccggcgag acggccggcg agaccgagga caaggagacc cgcgtcatcc
     3661 ggacgccgcc ggcatacggg acccaggcgg acgcgagccc ttatccgagg ggatacttcg
     3721 aggccgccga ggagcgcgag gacaggctca gggacatgta cgggggcgtg gactggctcg
     3781 ccagcttcat cggcttcatc ttcgcgctcg tcgccgggtc tttcttctcg ctcgtggccg
     3841 gcgtcgtggt ggctcccttg ggggtttcta tggaccttgc aggctcggag atagggcccg
     3901 ccgcgattac aggtctcgtg ctggtagcca tactcgtctt tctgacgtac ttcttcggcg
     3961 ggtacgtggc aggcaggctc gcgcgcttcg acggtggacg caacggggcc atgctgctcc
     4021 tgtggaccgg cgttacggtt ctagtcctcg cgcttatcaa cgcggtgttg agcggcttct
     4081 tgcccgaggg catctccgag aatattgaca gcctgatcca gaacttcctg ttctctacca
     4141 tcggcggcct ctcgggactc gggatcgcgg gcgttctgat cttcgttggc gcgctcctcg
     4201 ttgcgctcgt gggcggcttc ctcggcggac gcctcggcag ccgctatcac gccgagatag
     4261 accgcacgac ctgagaccgg cgctcgaccc attccccgta aatttacggt acggctgcgc
     4321 agggtataaa gcggtggcgg cgtaggctag gtaggtcgga tgggcgagca cgttctcttc
     4381 aaaacgggca taaggcggct cggctcgaag gagcggggct t
//
LOCUS       DASTUV010000133         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_848666, whole genome shotgun sequence.
ACCESSION   DASTUV010000133 DASTUV010000000
VERSION     DASTUV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848666"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFT03_02745"
     CDS             <1..205
                     /locus_tag="VFT03_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847092.1"
                     /translation="EKYIYHYGNKADEYFDLSSDPYERHNIIEQQSEEKIKALRDDLL
                     SWESRVTASYELQAAGEKKAAQE"
     gene            278..1057
                     /gene="fabI"
                     /locus_tag="VFT03_02750"
     CDS             278..1057
                     /gene="fabI"
                     /locus_tag="VFT03_02750"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEU4847093.1"
                     /translation="MGGLLEGKKVLVTGVLDRRSIAFSAARLASEQGAEVALSSFGRA
                     MNLTTRTAKRLDPEPPVLELDVNKPEDIEAVAQDLGERWGEIDGIVHAIAFAPEDALG
                     GNFLTARWPSVATALQTSAYSLKALAVGLADIMVEGSSVVGLDFDARVAWPAYDWMGV
                     AKAALESTSRYLARDLGNRGIRVNLVSAGPVKTLAAKGISGFEEIEGEWERQAPLGWD
                     TGNPEPVGRAVVSLLSDWWPATTGELIHVDGGYHAMGAPIK"
     gene            complement(1095..1841)
                     /locus_tag="VFT03_02755"
     CDS             complement(1095..1841)
                     /locus_tag="VFT03_02755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4847094.1"
                     /translation="MEGCDLPDYDPAAYGDRMAEVYDEWFGTPADAEETVAFLSDLAG
                     RGRVLELGIGTGRIALPLAGKGIEVHGIDASEAMVEKLRAKPSGDDIPVSIGDFADVG
                     VEGRFSLVYVVFNTFFALLTQEDQVRCFANVAERLEDGGAFVVEAFVPDPARLARGEV
                     TQTRRVEEDRVFLEASRYDPVHQRVYSQNIAMGETETRFYPVQIRYAWPSELDLMARL
                     AGLRLLQRWGGWRRERFTASSTSHVSVYKR"
     gene            complement(1873..2334)
                     /locus_tag="VFT03_02760"
     CDS             complement(1873..2334)
                     /locus_tag="VFT03_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU4847095.1"
                     /translation="MDGAVHQVRLPRPPRVGDLGVKDNTATVLRLIEDLFNTGDLDIA
                     DEVLASDYVDHSPSHPGLSGPENFKRSVSEWLTAFPDTVSVVRDIIAEGDRVAARWTT
                     QATHRSEFMDVPPTGNRIDVTWFGIFRLSGGRIVESWDTFNVVEMMRQLRR"
     gene            complement(2286..>2688)
                     /locus_tag="VFT03_02765"
     CDS             complement(2286..>2688)
                     /locus_tag="VFT03_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4847096.1"
                     /translation="SLSLVVVDYLQLMVSQGGRSENRQQEIAEISRGLKILARDLDVP
                     VLAIAQLSRAVEQRHDKRPLLSDLRDSGALEQDADMVMFLYRDEYYNPDSDDKGIAEI
                     IVGKHRNGPTGKVQLAWMEQYTKFASLARRV"
BASE COUNT          524 a          858 c          841 g          465 t
ORIGIN      
        1 cgagaagtac atctaccact acggcaacaa ggccgacgag tacttcgacc tgtcgagcga
       61 tccctacgag cgccacaaca tcatagagca gcagagcgag gagaagataa aagccctccg
      121 cgacgacttg ctctcgtggg agagcagggt cacagcctcc tatgaactcc aggccgctgg
      181 cgagaagaag gcggcgcagg agtagctcgg tagtcagggc gcggcgcata acctagacta
      241 ttccgcgtca cgggaaggcg agatctcggg aggacggatg ggtggactgc tcgaaggcaa
      301 gaaggtactg gtaacgggtg tcctcgaccg gcgctccatc gccttctcag cagcgcgcct
      361 ggcctcggag cagggcgcag aggttgcctt gagcagcttc gggagggcca tgaacctcac
      421 aacccgcacc gccaaacgcc tcgaccccga gccgccggtc ctggaactcg acgtcaacaa
      481 accggaggac atagaggccg tagcgcaaga cctcggagag cgctggggcg agatcgacgg
      541 tatcgtccac gcgatagcct tcgccccgga ggacgccctc gggggcaact tcctcaccgc
      601 caggtggccg tcggtcgcga cggcgctgca gacctcggcc tactcgctca aggccctcgc
      661 ggtcggtctt gcggacatca tggtagaggg ctcctcggtc gtcggcctag acttcgacgc
      721 cagggtagcc tggcccgcct acgactggat gggcgtggcg aaggccgccc tcgagtccac
      781 ctcccgctac ctcgcccgcg acctcggcaa cagaggcatc agggtcaacc tcgtatccgc
      841 cggcccggta aagaccctgg ccgccaaagg catcagcggc ttcgaggaga tagagggtga
      901 gtgggagcgg caggctcccc ttggctggga cactggcaac cccgagccgg tgggtcgggc
      961 tgtggtgagc ctgctctccg actggtggcc cgccacgacc ggcgagctaa tccacgtaga
     1021 cggcggttac cacgcgatgg gagccccgat caagtaggca tcaggtctca gcaaggtttg
     1081 gcggacggct tgcttcatct tttatagacc gagacgtggc tcgtgctcga ggcggtgaaa
     1141 cgttcgcggc gccagcctcc ccagcgctgg agcaaacgca gcccggccag gcgggccatg
     1201 agatctagct ccgacggcca ggcatagcgg atctggaccg ggtagaacct ggtctctgtc
     1261 tcacccatcg cgatgttctg cgagtagacg cgctggtgga ccgggtcgta gcgcgatgcc
     1321 tcgaggaaga cgcgatcttc ctcgacccgc ctggtctggg taacctctcc cctcgcgagt
     1381 cgcgcggggt cgggaacgaa cgcctcgacg acgaacgcgc caccatcttc cagacgctcc
     1441 gcaacgttag cgaagcagcg cacctgatct tcctgcgtca gcagcgcgaa gaaggtgtta
     1501 aagaccacat acaccagaga gaatctaccc tcgacgccga cgtccgcgaa atcccctata
     1561 ctcaccggga tatcgtctcc tgacggcttg gcccgcaact tctcaaccat ggcctcggag
     1621 gcgtcgatac cgtggacctc gatccccttg ccggccagcg ggagagcgat ccgacccgtg
     1681 ccgatcccga gctccaacac acgcccccgg cccgcgagat cgctcaggaa agcgacggtc
     1741 tcctcagcgt cggcaggggt gccgaaccac tcgtcgtaga cctcggccat cctgtcgccg
     1801 tacgcggcgg gatcataatc cggcaagtcg caaccctcca tttccaggac atcctacgcc
     1861 ggcgagatcg tatcaacgtc tcaactgtcg catcatctcg acgacgttga aggtatccca
     1921 actctcgacg atcctgcctc ctgagagacg gaagatgccg aaccacgtca cgtcgattct
     1981 gttgccggtc ggagggacgt ccatgaactc gctccgatgg gtagcctggg tggtccagcg
     2041 cgccgctacc ctatcaccct cggcgatgat gtcgcgtacc acgctaacgg tatcgggaaa
     2101 ggcggtgagc cactccgaca ccgatcgctt gaagttctct ggtccggaca ggccgggatg
     2161 cgaagggctg tggtcaacgt agtcgctggc caggacctcg tcggctatgt cgaggtcgcc
     2221 cgtattgaaa aggtcttcaa tgagtcgtaa gacggtggcc gtattatcct tgacgcctag
     2281 atctcctaca cgcggcgggc gagggaggcg aacttggtgt actgctccat ccaggcgagc
     2341 tggaccttgc ctgtgggtcc gttgcggtgc ttgccgacga tgatctcggc gatgcccttg
     2401 tcgtcagagt cggggttgta gtactcgtcg cggtagagga acatcaccat gtctgcgtct
     2461 tgttcaagag ctcccgaatc gcgcagatcg gagagcaggg gacgcttgtc gtggcgctgt
     2521 tcaacggctc gggagagctg ggctatcgcc aacacgggta cgtcgaggtc gcgggctaag
     2581 atcttgagtc cgcggctgat ctccgctatc tcctgctggc ggttctcaga gcgaccgccc
     2641 tggctcacca tgagctgcaa gtaatccacc accacgagcg agagcgac
//
LOCUS       DASTUV010000134         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1564556, whole genome shotgun sequence.
ACCESSION   DASTUV010000134 DASTUV010000000
VERSION     DASTUV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564556"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFT03_02770"
     CDS             <1..191
                     /locus_tag="VFT03_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847097.1"
                     /translation="TNDREARQTRTHVYLHLDGYGVYAQKTQADSAAYDHASEAPHVA
                     VTHLAHHPLPFPSQMRAV"
     gene            268..618
                     /locus_tag="VFT03_02775"
     CDS             268..618
                     /locus_tag="VFT03_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847098.1"
                     /translation="MEEARCALADEYADDVEAHGIEVWLLLGQISFCERADGGLLASG
                     DRVERATEARSPAQFHLGKDDSIAVAQNQVYLAEAGAVVALDELVALPGEVTQRELLA
                     PRAGGTFAQGLTPA"
     gene            complement(597..1223)
                     /locus_tag="VFT03_02780"
     CDS             complement(597..1223)
                     /locus_tag="VFT03_02780"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU4847099.1"
                     /translation="MAGTRSRNGSRKETPLYAFDAAWVAERSAPLAGADEAGRGALAG
                     PLVAAAVVLGEGEISGLNDSKTLSANVRESLFVEVLRRAEAASVVAFPAWWIDEFGVG
                     PANSEALRRAIVSLEPHAGCGLADGNLALGPEIECLPRADGTSAAVAAASVVAKVLRD
                     DAMRALAGEHAGYGFERNCGYGTPEHRLALAQLGPCRVHRLSYAGVSP"
     gene            complement(1233..1562)
                     /gene="rplS"
                     /locus_tag="VFT03_02785"
     CDS             complement(1233..1562)
                     /gene="rplS"
                     /locus_tag="VFT03_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU4847100.1"
                     /translation="MITSENLQQKDVTFKPGDTVRVHYRVIEGNRERIQVFEGLVIAR
                     KGGGINETFTVRKNSFGVAVERVFPLHSPKIAKIEVVSRGAVRRAKLYYIRDKVGRKA
                     RVKKAKK"
     gene            complement(1825..2502)
                     /gene="trmD"
                     /locus_tag="VFT03_02790"
     CDS             complement(1825..2502)
                     /gene="trmD"
                     /locus_tag="VFT03_02790"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU4847101.1"
                     /translation="MKSIDVFCVFPSVVDAALGVGVVGRAIERGAVGYRSFDLKEHAP
                     GGRIDDMPFGGGAGMVVRVDVVARALEAVYGVPAREVKEERRVVLTEAWGRPIDQRYV
                     NEVAEGPDLTIICGRYGGVDERVRDALATETVSIGDFVLSGGEIVAAALADAAIRRLE
                     GVLGNRDSLVGESFSGEGVIGPPPYTRPADWNGTPVPDVLLSGDHAKIKAWREEQARL
                     RVSQRDL"
     gene            complement(2507..3001)
                     /gene="rimM"
                     /locus_tag="VFT03_02795"
     CDS             complement(2507..3001)
                     /gene="rimM"
                     /locus_tag="VFT03_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU4847102.1"
                     /translation="MEPSDPVVIGHVVAPHGRRGTLRVRATGSGRHLREGAEPIIAGR
                     RRRISLVRQTPKGFLVDLEDVGDRPGAAALRGEGLVLDRSELDAPGEDEVYVDDLIGL
                     TAVGEAGEVFGTVQETFETPAHEVLVVREEKDAPARYVPFTREHVPDLDLASGRIIIR
                     PPEG"
     gene            complement(3006..3245)
                     /locus_tag="VFT03_02800"
     CDS             complement(3006..3245)
                     /locus_tag="VFT03_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU4847103.1"
                     /translation="MGQLEELVLVLARSLVDEPDKVEVSGTETDSRVDLELRVAQDDM
                     GKIIGRQGRTIRAIRTVAKAASVKLGKRVTVEVPD"
     gene            complement(3255..3497)
                     /gene="rpsP"
                     /locus_tag="VFT03_02805"
     CDS             complement(3255..3497)
                     /gene="rpsP"
                     /locus_tag="VFT03_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU4847104.1"
                     /translation="MAVKIRLARHGAKGAPFYRIVVADSRSPRDGRFIDRIGTYNPRT
                     EPSDIQVDADKARQWLDQGAQPTDQVRKLLKVSGVL"
     gene            complement(3523..4854)
                     /gene="ffh"
                     /locus_tag="VFT03_02810"
     CDS             complement(3523..4854)
                     /gene="ffh"
                     /locus_tag="VFT03_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU4847105.1"
                     /translation="MFDTLGERLNTALDGVRGSGNLTEDQVKKVTREIRLALLEADVN
                     YSVVKEFVGNVRERATGAEVSKALSPGQQVVKIVNEELASLMGGTAHKLSFAGRPPTV
                     VMLAGLNGHGKTTVAGKLALFVRKMGKNPYLVACDTYRPAAIDQLQQIGRELGVPVYS
                     EGTEPAPEEIAERGIKAARDAGYDFVIVDTAGRQVVDVEMMEELKRVRKASKPHSVLL
                     VLDVVTGQNAVEVAQAFQEYADFDGMVLTKLDGDARGGAALSVVSVTGRPVRFASEGE
                     KMSEFDYFYPDRMAGRILGMGDVLTLIEKAEQQMDREEAQADARRLMSGKFTFEDFLK
                     QMQMLKKMGPLSGLLGMIPGMGKQLQGAQIDDRMLGRVEAMVTSMTARERQNPKLLQN
                     QSRVRRIARGSGSTPQEVQKFIKQFNEMQKMMRQMGKGGGMPRMPGLPGRR"
     gene            complement(4856..>5244)
                     /locus_tag="VFT03_02815"
     CDS             complement(4856..>5244)
                     /locus_tag="VFT03_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU4847106.1"
                     /translation="EAAKRDGVDVLLVDTAGRLHTKINLMAELDKVKRVIGRQLPGAP
                     HEVLLTVDATTGQNGLRQARMFNEVAGVTGVILTKLDGTAKGGIALAIANEIGVPIKL
                     ISVGEKAEDLHPFDAREFAEALFGDL"
BASE COUNT          818 a         1833 c         1602 g          991 t
ORIGIN      
        1 aaaccaacga tcgagaagcc aggcaaaccc gcactcacgt ctacctccac ctcgacgggt
       61 acggcgtcta tgcccagaag actcaggctg acagcgcggc atacgaccat gcctcagagg
      121 ccccacacgt cgccgtaacg cacctcgccc accatcctct gcctttccct tcgcagatgc
      181 gggcagtata aagcaagtgg tatttgtttt acaagagtaa attttactgt acgaaaatct
      241 ggagcgctct cagaaggcgt ccggcacgtg gaggaggcga ggtgtgccct cgcggacgag
      301 tatgccgacg acgtcgaagc gcacggtatc gaagtctggc tccttctcgg ccagatatcg
      361 ttttgcgaga gagcggatgg cggcttgctt gcgagcggtg accgcgtcga gcgggctacc
      421 gaagcccgtt ccccggcgca gtttcacctc ggtaaagacg atagtattgc cgtagcgcag
      481 aatcaggtct atctcgccga agcgggtgcg gtagttgcgc tcgacgagct cgtagccctt
      541 ccgggtgagg taacgcagcg cgagctcctc gccccacgtg ccggcggaac gtttgctcag
      601 gggctgactc cggcgtagct caggcgatgg acccggcagg gaccgagctg ggcgagcgcg
      661 agccggtgct cgggcgtccc gtagccgcag ttcctctcga agccgtatcc tgcatgctca
      721 ccggcgaggg cgcgcatggc atcgtcgcgc aagacctttg cgacgacgct cgccgctgcg
      781 acggctgcgc tcgtgccgtc cgccctgggc aggcactcta tctcgggacc gagcgccagg
      841 ttgccgtcgg cgaggccgca accggcgtgg ggctcgagcg agacgatggc ccggcggagc
      901 gcttcactgt tggccggacc gacaccgaac tcatctatcc accaggccgg gaaggctacg
      961 acagaggcgg cttcggcgcg gcgcagcacc tcgacgaaga ggctctcgcg gacgttcgcg
     1021 ctcagggttt tggagtcgtt gaggccggag atctcacctt ctccgaggac caccgcggcg
     1081 gccacgagcg gccccgcgag ggcgccccgg cccgcctcgt cggccccggc cagcggcgcg
     1141 ctgcgttcgg ccacccacgc ggcgtcgaag gcgtagaggg gggtttcctt gcgggagccg
     1201 ttcctcgagc gggtcccggc catcgctctt cgttacttct tggccttctt gaccctcgcc
     1261 ttgcggccga ccttgtccct gatgtaatag agcttggcgc gacgcaccgc gcccctggag
     1321 acgacctcta tcttcgctat cttgggcgaa tgaagcggga agaccctctc cacggcgacg
     1381 ccgaaggagt tctttctgac cgtgaacgtc tcgttgatgc ccccgccctt gcgggctatg
     1441 accaggccct cgaatacctg tattcgttcg cggttgccct ctatgacgcg gtagtgcacc
     1501 cgtacggtgt cgcccggctt gaacgtgacg tccttctgct gcaaattctc gctcgttatc
     1561 atgctcgctc ctctcgtggg tggataactt taaggccaag gagaccttcc tccggtcgcc
     1621 cgcaccctac tccctcgcaa ggcgtgctgg cgcgggtctc ggaagttctc gcgattgccg
     1681 gataatagca tgccgaagag cggctctcca tctcgggttc tcaggccatg gcgcatctcc
     1741 cgaagcagat ttcgcgcgag agcacagccg ccaggggatg tgtcccgagc cggagttccc
     1801 gcgggttctg gacgcgatta attttcagag gtctctttga gagactctca ggcgtgcctg
     1861 ttcttcccgc caggctttaa tcttcgcgtg gtcgccggaa aggagcacgt cggggaccgg
     1921 tgtgccgttc cagtcggcgg ggcgggtgta cgggggaggt ccgatcacgc cttctcccga
     1981 gaacgactcc ccgaccaggc tatccctgtt cccgagcacg ccttcgagac gcctgatggc
     2041 ggcgtcagcg agcgctgccg ccacgatctc ccctcctgag agcacgaagt cgcctatcga
     2101 gacggtctcc gtcgcgaggg catcgcgcac ccgctcgtcg acgccgccgt agcggccgca
     2161 gatgatcgtg aggtccggcc cttcagcgac ctcgttgacg tagcgctggt cgatcgggcg
     2221 cccccaggcc tccgtgagga cgacgcgccg ctcctccttg acttccctcg cggggactcc
     2281 atacaccgcc tcgagcgccc tggcgacgac gtccacgcgc accaccatcc ccgctccgcc
     2341 cccgaagggc atgtcgtcta tgcggccgcc cggagcgtgc tccttgaggt cgaagctgcg
     2401 gtagccgacg gcaccacgct cgatcgccct gccgacgacg ccgacgccca gagcggcatc
     2461 taccacggag gggaacacgc agaacacgtc gatggacttc acggcgtcag ccctccggcg
     2521 gacggatgat aatgcgtccg gaggcgagat ccaggtccgg cacgtgctcg cgggtgaagg
     2581 gcacgtaacg cgccggggcg tctttctcct cgcgcactac caggacttcg tgggccgggg
     2641 tctcgaacgt ctcctgcacg gtcccgaaca cctcgccggc ctcgccgacg gcggtaagcc
     2701 cgatcaggtc gtccacgtag acctcgtcct cgccgggcgc atcgagctcg gagcggtcga
     2761 gaacgagccc ttcccccctc agggcggccg cccctgggcg gtccccgacg tcttcgaggt
     2821 ccaccaggaa accttttggg gtctggcgta ccagggagat gcgcctgcgg cggccggcga
     2881 tgatcggctc ggcgccttct cgcaggtgcc tgccggagcc ggtggcgcgc accctcaggg
     2941 tcccgcgcct gccgtggggc gcgaccacgt gaccgatcac caccggatcg gagggctcca
     3001 cgcccctagt ccgggacctc cacggtgacc cttttgccca gcttgaccga ggcggccttg
     3061 gcgaccgtcc ggatcgcccg tatcgtgcgt ccctggcggc cgatgatctt gcccatgtca
     3121 tcctgagcca ccctgagctc caggtccacg cgcgagtcgg tctcggtgcc cgagacctct
     3181 accttgtcgg gctcgtcgac gagcgaacgc gccaggacga gcacgagctc ttcgagctgt
     3241 cccacgccgc gtccttaaag gaccccggag accttcaaca gcttccggac ctggtccgtt
     3301 ggctgggccc cctggtcgag ccactggcgc gccttgtccg cgtccacctg gatgtctgag
     3361 ggctccgtgc gcgggttgta ggtcccgatc cggtctatga accggccgtc gcgcgggctc
     3421 ctcgagtcgg cgaccactat cctgtagaat ggcgcgccct tcgcaccgtg cctggccagc
     3481 ctgatcttta ccgccatctt ctctcctctc gtaggtgcaa acttacctgc gtccgggcag
     3541 gcccggcatg cggggcattc cgccgccctt gcccatctgc cgcatcatct tctgcatctc
     3601 gttgaactgc tttatgaact tctggacttc ctgaggggtc gacccggagc cgcgggcgat
     3661 cctacgcacc ctgctctggt tttgcaagag cttggggttc tgccgctcgc gggcggtcat
     3721 cgacgtcacc atcgcctcga ccctgccaag catccggtcg tcgatctgcg cgccctgcag
     3781 ctgcttgccc atgcccggaa tcatgcctag aagccccgag agcggaccca tcttcttgag
     3841 catctgcatc tgcttcagga agtcctcgaa ggtgaacttc ccggacatga gccgcctggc
     3901 gtcagcctgg gcctcctcgc ggtccatctg ctgctcggcc ttctcgatca gcgtgaggac
     3961 gtcgcccatc ccgaggatgc gtcctgccat ccggtcaggg tagaagtagt cgaactcgct
     4021 catcttctcg ccctcggagg cgaagcggac agggcgcccc gtaaccgaga ccacagaaag
     4081 ggcggcccca cccctcgcgt cgccgtcgag cttggtgagg accatcccgt cgaagtccgc
     4141 gtactcctgg aaagcctgcg cgacctcgac cgcgttctgg cccgtcacta cgtcgagcac
     4201 cagaaggacg gagtggggct tggatgcctt tcgcaccctc ttgagctcct ccatcatctc
     4261 cacgtctacg acctgccggc ccgccgtgtc aactatgacg aaatcgtagc cggcgtccct
     4321 ggcagccttg atgccgcgct cggcgatctc ttccggcgcc ggctccgtgc cctcggagta
     4381 aacaggcacc ccgagctcgc gcccgatctg ctgcagctgg tctatcgctg ccggtctata
     4441 tgtgtcgcaa gccacgaggt acgggttctt gcccatcttg cgcacgaaca gcgccagctt
     4501 gcccgcaacc gtcgtcttgc cgtgcccgtt gaggccagcg agcatcacga cggtcggcgg
     4561 ccggccggcg aaagacagct tgtgggccgt gccgcccatg aggctcgcca gctcctcgtt
     4621 gacgatcttg acgacctgct ggcccgggct gagcgccttc gagacctcgg ccccggtcgc
     4681 tctctccctc acgttgccga cgaactcctt gacaaccgag tagttgacgt cagcctcgag
     4741 caaggcgagc cgtatctcgc gcgttacctt ctttacctgg tcctcggtga ggttcccact
     4801 gccgcgcacc ccgtcgagcg cggtgttcag cctctctcct aacgtgtcaa acatgctaga
     4861 gatctccaaa caaagcttcg gcgaactcgc gggcgtcgaa cgggtgcagg tcctcagcct
     4921 tctcgccgac cgagatcagc tttatgggaa ccccgatctc gttggctatg gccagcgcta
     4981 tgccgccctt ggccgtgccg tcgagcttgg tcaggatgac ccccgtcacc cccgcgactt
     5041 cgttgaacat ccgcgcctgc ctgagtccgt tctggcccgt tgtggcgtcc acagtgagca
     5101 ggacctcgtg cggcgcgcca gggagctgcc tcccgatgac gcgctttacc ttatccagct
     5161 ccgccatcag gttgatcttg gtgtgcaacc gcccagccgt gtcaacgagc agaacgtcaa
     5221 ctccatctcg ctttgccgct tcga
//
LOCUS       DASTUV010000135         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1559276, whole genome shotgun sequence.
ACCESSION   DASTUV010000135 DASTUV010000000
VERSION     DASTUV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559276"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /gene="der"
                     /locus_tag="VFT03_02820"
     CDS             complement(<1..1255)
                     /gene="der"
                     /locus_tag="VFT03_02820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU4847107.1"
                     /translation="MAVVGAPNVGKSSLVNRIMGRRGAVVSDEPGTTRDRGYNRAEWA
                     GRDFVLVDTGGMEPAARVGLQSLVTLQANVAVEEADVILHVVDARLGTTEADAAIARQ
                     LRRASGKVVLAVNKLDNPSDDTERYGFYALGEGEPHAISALHGIGVGDLLEEVIGLLP
                     DSEPDEEDRLPRLAIVGRPNVGKSTLLNRLLGAERAVVSEEAGTTTDAVAGEVEVEVN
                     GQPVRFLVLDTAGVSRTAKRASGVPYYSSLRTAEAIRRSEVSLLLVDAVNGLVAGDLA
                     IARQIEEAGSSCGVLINKRDLVPRERIREIEGQLFARMTDLKPPALFISALAGEGVDQ
                     VLPLAARLHLAYNLRIPTHEVAEFINEVVARNAPPRGVKIRYATQTGTAPPRFTLFAN
                     RPKDLPDSYLRYVENSLRERYDLWGV"
     gene            complement(1277..1915)
                     /locus_tag="VFT03_02825"
     CDS             complement(1277..1915)
                     /locus_tag="VFT03_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU4847108.1"
                     /translation="MEVLETPRNRSTPEEMSSRYKLFRRMMLVLGRILWGFTVHGAEK
                     VPRKGALVVAANHSQYLDPVYVCMAIPRRVQWMGKKELFAFPFRAFFYFIGTFPVDRE
                     GDSRAGLRTALNFLARGWTLGIFPEGTHRTGEDSRAAKSGTVLLAVRAGAPVVPVFVG
                     RSPGLRGRLRGEKLHVYVGDKITLDPTLRGRDEYRAAAEEVLRTVYALPGGA"
     gene            complement(1915..>2553)
                     /gene="cmk"
                     /locus_tag="VFT03_02830"
     CDS             complement(1915..>2553)
                     /gene="cmk"
                     /locus_tag="VFT03_02830"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU4847109.1"
                     /translation="PAGSGKSSAARAIADELGFINLNTGAAYRAVALVALQEDVSLDD
                     GASLAGVSRRVSLDAGGARVDGKPVPEDELRTPEVSAAASAVSARPEVRAVLLDVQRE
                     AARRANREGGAVVEGRDIGTVVLPEAELKVYLSAAPEERARRRAHQTGREAELERIKE
                     AMSQRDRQDSERETSPLKPAPDAVILDTTSLSLEGVVSRVLELVRDLREKGA"
BASE COUNT          379 a          919 c          820 g          435 t
ORIGIN      
        1 ttacccccca cagatcatac ctctccctga gagagttctc cacgtagcgc aggtaagaat
       61 cgggcagatc tttcggcctg ttggcgaaga gcgtgaagcg cgggggcgcc gtcccggtct
      121 gggtggcgta ccggatcttg acgccccttg gcggggcgtt gcgcgcgact acctcgttta
      181 tgaactccgc gacttcgtgg gtcgggatgc gcaggttgta agccaggtgg agccgggcag
      241 cgaggggaag tacctgatcc acaccctcgc cagcgagcgc ggagatgaaa agcgcgggcg
      301 gcttcaggtc ggtcatgcgg gcgaagagct ggccttcgat ctcccggatc ctctccctgg
      361 gtaccaggtc ccgcttatta atgagtaccc cacacgagga gccggcctct tctatctggc
      421 gcgcgatggc gaggtcgccc gccacgagcc cattcacggc gtcaacgagg agcagcgaga
      481 cctcagatcg cctgatggcc tcggccgtcc tcagcgagga gtagtagggt acgccgctcg
      541 cgcgttttgc ggtgcggctc acccctgcgg tgtcgagcac gaggaagcgc accggctggc
      601 cgtttacctc cacttcgacc tcgccggcga cggcgtcggt agttgtgccc gcctcctcgg
      661 agacgaccgc cctctcagcg ccgagcaggc ggttcaggag cgtgctcttg cccacgttgg
      721 gacggccgac tatggcgagc ctcggcaacc tgtcttcctc gtccggctcg gagtccggca
      781 ggagcccgat tacctcctcg aggaggtctc ctaccccgat gccgtgcagc gccgagatcg
      841 cgtgcggctc cccctcgcct agcgcgtaga atccgtagcg ctcggtgtcg tcagaggggt
      901 tatccagctt gtttaccgcg aggacgacct tgccgctcgc cctccttagc tggcgggcga
      961 tcgccgcgtc cgcctccgtc gtcccgagcc tggcgtcgac gacgtgcagg atcacgtcgg
     1021 cttcctcgac ggctacgtta gcttgcagcg taacgagcga ctgcagaccg accctggcgg
     1081 cgggctccat cccgcccgta tctactagta cgaagtccct gccggcccac tcggcgcgat
     1141 tgtaccccct gtcgcgcgtg gttcccggct cgtcggagac cacggctccg cggcgcccca
     1201 tgatgcggtt gaccagggag ctcttgccga cgttgggcgc ccctactacc gccacgactg
     1261 gcgccctgcg caccgtctaa gccccgccgg gcagggcgta gacggtgcgt agcacctcct
     1321 cggccgctgc ccggtactca tccctgccgc gcagagtggg gtccagggtg atcttgtccc
     1381 cgacgtagac gtgaagcttc tcgccgcgca gcctgccgcg caggcccgga ctcctgccaa
     1441 caaatacggg cactaccggg gcgcccgcgc gcaccgcgag cagaacagtc ccgctcttgg
     1501 ccgccctcga gtcctcccct gtgcggtggg tcccctcggg gaagatcccg agcgtccatc
     1561 ccctcgccag gaagttgagg gcggtccgca acccggcgcg gctgtcgccc tcgcggtcca
     1621 cggggaaggt gccgataaag tagaagaatg ccctgaacgg aaaggcgaag agctccttct
     1681 tgcccatcca ttgcaccctg cgcgggatgg ccatgcaaac gtagacggga tccagatact
     1741 gcgagtggtt ggccgcgacg acaagagccc ctttgcgcgg aactttctcc gcgccgtgca
     1801 cggtgaaccc ccacaggatt cgtccgagca cgagcatcat ccgcctgaac agcttgtagc
     1861 gcgaggacat ctcctccggt gtgctccggt tcctgggagt ttccaggacc tccatcacgc
     1921 tcccttctca cggaggtcgc gcacgagctc cagcacgcgc gagaccaccc cttcgaggct
     1981 cagagaggta gtatcgagga tgacggcgtc gggggctggc ttgagcgggg aggtctcgcg
     2041 ctcggagtcc tggcggtcgc gctggctcat ggcctccttg atgcgctcca gctcagcttc
     2101 gcggcccgtc tggtgcgccc tcctgcgcgc ccgctcctcc ggcgccgccg agaggtacac
     2161 cttgagctcc gcctcgggca gcactaccgt gccgatgtcg cggccctcca ccacagcccc
     2221 gccctcccgg ttcgccctcc gcgcggcctc ccgctgcacg tcgaggagca cggcccgcac
     2281 ctcgggcctc gccgagacag ccgaggccgc ggccgagacc tccggcgtgc gcagctcgtc
     2341 ctcggggacg ggcttcccgt ccacgcgagc gccccccgcg tcgaggctga cacggcggga
     2401 gacgccggct agagacgccc cgtcatcgag gctcacgtcc tcctggagcg ccacgagagc
     2461 cacggcccgg tatgcggccc ccgtgttcag gttgatgaag ccaagctcgt ctgcgatcgc
     2521 ccgcgctgcc gagctcttgc cgctcccggc cgg
//
LOCUS       DASTUV010000136         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1387367, whole genome shotgun sequence.
ACCESSION   DASTUV010000136 DASTUV010000000
VERSION     DASTUV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387367"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1240
                     /locus_tag="VFT03_02835"
     CDS             281..1240
                     /locus_tag="VFT03_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4847110.1"
                     /translation="MPYNYCSQRKGRENNLGSTENRLLGALAVAAAATMWGTLGIFAK
                     ILYAQGVSFESLVAVRASVGWLAVIGFLLATRGARALRVERRDLAFLLPLGLVGIGAF
                     YLLYFYTVRESTVGTAAILLYSAPAFVVVLAWLFLREPLNAAKVFGLLLTVGGISLVV
                     GAYDPANLEVSTKIVLTGLLSGLTYGLYAIFGRPIAGHLSPPVILSYALAFGSALLVV
                     AALPTLDTLVGLPATSYLILLMLSVVHTTLAFALYTFGIRHLGAGRAAIVATIEPVVA
                     GVLGITLLGEELTVLKALGAVLVISGAILAQLRAGKPARIDPA"
     gene            1639..2766
                     /locus_tag="VFT03_02840"
     CDS             1639..2766
                     /locus_tag="VFT03_02840"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU4847111.1"
                     /translation="MPIEFKAGGSDYTLGVEEELHVVDATTAELAPKIDEIMARLPED
                     LAEFVSYELFQSVLEIKTPVCRTAAEAEKHLRELRGRIASWAAACDASLAAAGTHPFS
                     RYKDQKVTDQERYRKVIETLRWIAQREVIFGQHVHVGVPGPEECIQAHNRLSEHAPLL
                     LALSANSPYWQGMDTGYESTRVKIFESFPRAGMPPAFPDYESFEAYVDLMVEAGAMED
                     YTYCWWDVRPHPKIGTIELRIPDTQTSLRPAVALTAMTQCLVARSLEETGPQGPYDRE
                     LAIENKMRAARHGMDAAFYDAKERVTVPAREMARSLVEQLRPISQDLSCENELLNVLE
                     IVEGGTGSQRQRRTYEESGDFLDVVAFLIEGTRPALAEEQS"
     gene            complement(2794..3075)
                     /locus_tag="VFT03_02845"
     CDS             complement(2794..3075)
                     /locus_tag="VFT03_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU4847112.1"
                     /translation="MVTAIVLVTTEGERVQEAAQAMLGIEGVTEAYSVTGPYDLVVMV
                     RIPDFERLAEIVPEKIAQVPGVARTETMVAFRAYSNYDLDRAWSLGFEE"
     gene            complement(3088..>3672)
                     /locus_tag="VFT03_02850"
     CDS             complement(3088..>3672)
                     /locus_tag="VFT03_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847113.1"
                     /translation="SFPGTLATVAEDLRLSDGIVRHHYLPAVQDALEEADQERDQSYL
                     SEPEPFMDYEEIGFKVAPAAVSAARDDPNALHGVEISAVEVAGQPLYQGGNVILTGSD
                     VPIFFVTVTNGGEVPETGVDVEVILNTRAERQDQTATIGQLRSNGGFATVQVGGFRPG
                     ELEETADVRVEAGPVKYEENSKNNTLTGTVTFGL"
BASE COUNT          606 a         1198 c         1188 g          680 t
ORIGIN      
        1 gagcgggttc tgttcctggg agcggtctct tgcgccctgg caagcgcatt cttctggctg
       61 ctcgcggtgc gcgccgggga tcgtgggctc gcgtggatga cggcggtggc aaccgtggtg
      121 tgctgcgtcg cggctctttt cctgttgaga atgctgtgaa gagatattga gcgtgtggca
      181 ccccgaccat ccataactct cgccgagctg cgcccaatgc aactttggat aagtagcagg
      241 gagagacagc gcctccaaag ctactgcgcc gcaactgcgt ttgccttata actactgttc
      301 gcagaggaag ggacgggaga acaacctggg ctcgaccgaa aacagattgc tcggggcgct
      361 ggcggtcgcc gctgcggcga ccatgtgggg cactctgggg attttcgcca agatcctcta
      421 cgctcagggc gtctctttcg agtcgctggt cgcggtgcgg gcctctgtcg ggtggctggc
      481 cgtcatcggt ttcctgctgg cgacgcgggg cgcgcgggct ctcagggtcg aacggcggga
      541 tcttgccttc ttactacctc tgggactggt cgggatcgga gcgttctacc tgctctactt
      601 ctacacggtg cgcgagagca ccgtgggcac agcggcgatc ctgctctaca gcgcacccgc
      661 cttcgtggtc gttctggcgt ggctgttctt gcgggagccg ctgaacgcgg cgaaggtatt
      721 cgggctgctg ctcacggtcg gtgggatctc tctggtcgta ggcgcctacg acccggctaa
      781 cctggaggtg agcacgaaga tagtgctcac cggcctgctc tccgggctga cctacggact
      841 ctacgcgatc ttcggcaggc ccatcgcagg ccacctgagc ccgcccgtga tcctgagcta
      901 cgccctggct ttcggctccg cgctgctcgt cgtcgcggcc ctcccgacgc tcgacacgct
      961 cgtgggactg ccggctacct cttacctgat cctgttgatg ctctcggtgg tacacacgac
     1021 gctggctttc gcgttgtaca ccttcggcat cagacacctc ggcgcggggc gggccgcgat
     1081 cgtcgcgact atcgagcccg tcgtggccgg ggtcctcgga attacgctgc tcggggagga
     1141 gttgaccgtg ctgaaggcgc tgggcgccgt gctggtgata tcaggcgcga tactcgccca
     1201 gctcagggct ggcaagcctg ctcggatcga tcccgcctga gcggcgcgtc gacgcgctga
     1261 gcggcctgtc ggagcgaggg aggtgtgcag gcccagagca agaaggccca tgtcccgaga
     1321 agacgagcga ggcgcttggg ctcgaaaggg cattgccgat tgcaagagac cgctcttggc
     1381 ctttatccat caaaacgttt atagtatcgc tcgctagggg agagatcgtc taggggattc
     1441 cgtgaagcgc accgttacat ggttggcata gacggagagg acgatccgga gaaggtgcgg
     1501 cgattcttca ggagtatggc atcgagagcc cgacggtcta cgagaccaac ctggctactc
     1561 tacgactcgt gtgctggaca gcgactaccg ggtggccggc gcccactcgg aggagcccac
     1621 tagggtgaag gaggcgccat gccgatagag ttcaaggccg gcggttcgga ctacactttg
     1681 ggcgtcgagg aggagctcca cgtcgtggac gccacgaccg cggagctggc gccgaagatc
     1741 gatgagatca tggcccgcct cccggaagac ctggccgagt tcgtctccta cgagctattc
     1801 cagtctgtcc ttgagatcaa gacgcctgtg tgccggaccg cggccgaggc cgaaaagcac
     1861 ctgcgcgagt tgcggggcag gatcgcctcc tgggccgcgg cctgcgacgc ttctctggcc
     1921 gccgccggca cccacccctt ctcacgttat aaggaccaga aggtcacgga tcaggagcgc
     1981 tacaggaagg tcatagagac cctgcgctgg atcgcccagc gggaggtgat cttcggccag
     2041 cacgtccacg tcggcgtccc cggcccagaa gagtgcatcc aggcccacaa ccgcctctcg
     2101 gagcacgcgc ccctcctcct ggcgctctcg gccaactccc cttactggca ggggatggac
     2161 acgggctacg agtcgaccag ggtgaagatc ttcgagtcct tcccccgcgc gggcatgcct
     2221 ccggccttcc ctgattacga gtccttcgag gcctacgtgg acctgatggt cgaggccggg
     2281 gccatggaag actacaccta ctgctggtgg gacgtcaggc cccatccgaa gataggcacg
     2341 atcgagctgc gcatcccgga cacccagacg agcctgagac ccgcggtcgc cctgaccgcc
     2401 atgacccagt gcctcgtcgc ccgttccctc gaagagaccg gcccgcaagg cccctacgac
     2461 cgcgagctgg ccatagagaa caagatgcgt gccgcgcgcc acggcatgga cgcggccttc
     2521 tacgacgcca aggagagggt caccgtcccg gcccgcgaga tggcccgctc cctcgtcgag
     2581 caactccgtc ccatttccca ggacctgagc tgcgagaacg aactgcttaa cgtgctcgag
     2641 atagtcgagg gcggcaccgg cagccagcgc cagcgccgga cctacgagga gagcggagac
     2701 ttcctcgacg tcgtcgcctt tctcatagaa ggtacccgtc ccgccctcgc cgaagaacag
     2761 agctaggctt cggtagggtc gatggcgtag aacctactct tcgaacccca gcgaccacgc
     2821 cctgtcgagg tcgtagttcg agtatgcgcg gaacgcgacc atggtctcgg tgcgggcgac
     2881 gccggggacc tgggcgattt tttcggggac tatctcggcg agccgctcga agtcggggat
     2941 gcgcaccata acgacgaggt cgtagggacc cgtcacggag tacgcctcgg tgacgccctc
     3001 tatcccgagc atcgcctggg ccgcctcctg taccctctct ccctccgtcg tcacgagcac
     3061 gatcgccgtt accacgccgg tcctccccta caacccaaag gtcaccgtcc ccgtgagggt
     3121 gttgtttttc gagttctcct cgtacttcac cgggccggcc tcgaccctca cgtctgcggt
     3181 ctcctccagc tcgccgggtc tgaaaccgcc gacctgcact gtggcgaacc cgccgttgga
     3241 ccgcaactgc ccgatggtcg ccgtctggtc ctggcgctcg gcccttgtgt tcaggatgac
     3301 ctcgacgtca acgcccgtct cgggcacctc gccgccgttg gtgacggtga cgaagaagat
     3361 cggcacgtca gagccggtca ggattacgtt cccgccctga tacaggggct gcccggccac
     3421 ttcgacggcc gagatctcga cgccgtgcag ggcgttcggg tcgtcgcggg cggcgcttac
     3481 ggccgccgga gccaccttga agccgatctc ctcgtaatcc atgaacggct ccggctcgct
     3541 caggtagctc tggtcgcgct cctggtccgc ttcttcgagg gcgtcctgaa cggccgggag
     3601 gtagtggtgc ctgacgatgc cgtcgctgag gcggaggtcc tccgccacag tagcgagcgt
     3661 ccccgggaag ct
//
LOCUS       DASTUV010000137         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1237182, whole genome shotgun sequence.
ACCESSION   DASTUV010000137 DASTUV010000000
VERSION     DASTUV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237182"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..>2297)
                     /locus_tag="VFT03_02855"
     CDS             complement(137..>2297)
                     /locus_tag="VFT03_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU4847114.1"
                     /translation="PDHWPLARGEVIFAGEPVAVVLATDRYKAQDALEFIEVNYEPMS
                     VVTDIEKALEDGAPLVHEKLGTNECYIWTLGTGDVEDAFSKADVVVSERYIQQRLIPS
                     AIETRAVVVQPAPLGGEFTMYTSTQVPHFVKDILSVMCGVPDTKLRVVAPDVGGGFGS
                     KLNVYSEEVLALVLARKLGVPVKWVEDRSENHLATTHGRGQVQYIELAATNEGKILGM
                     KVKLLADVGAYLQLLGPGIAIFGAFTYPGLYTFDAYSFECTGVFTNLTPTDAYRGAGR
                     SEAAYAHERIMDALARELDMDPAELRLKNLMPPFDEPTMTPAGVQYDSGNYETCMRKA
                     LELVEYEEARAEQKRRRESGDVKQIGIGIGNFTESGGLSPSKVTAAVRLQSAGWEAAS
                     VRILASGKVEVITGTSPHGQGHVTSWSQIAADALGVDVDDVEVLHGDTAIAPYGRDTY
                     GSRSLPVGGVAVHLACEKVVDKARKLAAHMLEAAEEDIEFEAGRFSVAGSPNQNVTIQ
                     DVAGAAYLADNLPEGMEPLLNADYVFDPPNFTWPYGTHVCVVEVDTETGMVTIPKYVA
                     VDDCGPVINPAIVDGQLHGGIAQGIGQALYEEALYDEDGNLVTGTMVDYLIPGAPEVP
                     NMILDRTETPSPTNPMGVKGIGESGAIAAQPAVINAVIDALSHEGVTHIDMPASPMRV
                     WQTLQAARGRDARSRDADLSGRPSLSREPMQDSVDEV"
BASE COUNT          374 a          751 c          734 g          438 t
ORIGIN      
        1 tccgagaggt ggccgagctg gctggttaca ccaccaagta ggtcaagaga tcgcccggcg
       61 ttaccaggag ctgggggttg agggcttggg tgaccgccgg cacaaaaacc ccggcgcttc
      121 cctcctcagg cttgggctaa acctcatcca cactgtcctg catcggctcc ctcgacagcg
      181 acggacggcc cgagaggtcg gcgtcacgcg acctcgcgtc ccggcctcga gccgcctgta
      241 gggtctgcca cacccgcatt ggggaggccg gcatgtcaat gtgggtgacg ccctcatgcg
      301 agagcgcatc tatgaccgcg ttgatgacgg ccggttgggc ggctatggcc cccgactcac
      361 ctatgccctt cacgcccatt gggttggtcg gggagggggt ctctgtccgg tcgaggatca
      421 tgttcggtac ctcgggggcg ccagggatca ggtagtccac catcgtcccg gtcaccaggt
      481 tgccgtcctc atcgtagagt gcctcctcgt agagagcctg tcctatcccc tgagctatgc
      541 cgccgtgcaa ctggccgtcc acgatggccg ggttgatcac cggcccgcag tcgtccaccg
      601 ccacatactt ggggatcgta accatcccgg tctccgtgtc cacctcgaca acgcagacgt
      661 gcgtcccgta aggccaagtg aagttcggtg gatcgaagac gtagtcagcg ttgagcaatg
      721 gctccatgcc ttcggggagg ttgtccgcca ggtaagcggc tccggcgacg tcctggatcg
      781 tgacgttctg attcggagat cccgcaacgg agaaacgccc ggcctcgaac tcgatatcct
      841 cttcggcagc ctccagcatg tgcgccgcta gcttcctggc cttgtccacc accttctcgc
      901 aggccaggtg caccgcgacg ccgcccacgg gcaggctcct ggagccatag gtgtcgcggc
      961 cgtacggggc tatggcggtg tcgccgtgga gtacctccac gtcatccacg tcgacgccga
     1021 gtgcgtcggc agcaatctgc gaccaggacg taacgtggcc ttggccgtgg ggcgaggtgc
     1081 cggttatgac ctctaccttg cctgaggcga gtatccgcac ggaagccgcc tcccagccgg
     1141 cgctctgcaa gcggactgcc gcggtgacct tcgaggggga gaggccgcca ctctcggtga
     1201 agttaccgat cccgatcccg atctgcttta cgtctcccga ttcgcggcga cgcttctgct
     1261 cggcgcgcgc ttcttcatac tcgacgagct ctagggcctt gcgcatgcag gtttcgtagt
     1321 tgccggagtc gtactgcaca ccggcggggg tcatagtcgg ctcgtcgaag ggtggcatca
     1381 ggttcttcaa ccgcagctcc gccggatcca tgtcgagctc gcgggcgagg gcgtccatga
     1441 tccgctcgtg ggcgtaggct gcctcggagc ggccggcgcc gcggtaggcg tctgtcggcg
     1501 tgaggttggt gaagacgccg gtacattcga aagagtaggc gtcgaaggta tacaggccgg
     1561 ggtaggtgaa ggctccgaag atggcgatgc cggggccgag gagctgcaaa taggcgccca
     1621 cgtctgccag aagtttcacc ttcatgccga ggatcttgcc ctcgttcgtc gcggcgagct
     1681 ctatgtactg gacctgcccc cggccatggg tggtggccag gtgattttcg gagcggtcct
     1741 cgacccactt taccggcacc ccgagcttac gcgccagcac gagcgccagc acctcttccg
     1801 aatacacgtt cagcttggag ccgaagcccc cgcctacgtc cggagccacg acccggagct
     1861 tggtgtccgg caccccgcac atcacggaga gtatgtcctt cacgaagtgc ggaacctggg
     1921 tcgaggtgta catggtgaat tcgccgccaa gcggcgcggg ctgcactacg acggcccgag
     1981 tctcgatggc gctcgggatc aggcgctgct ggatgtagcg ctcgctcacc accacgtccg
     2041 ccttggagaa ggcatcctcg acgtccccgg ttccgagtgt ccatatgtag cactcgttgg
     2101 tgccgagctt ttcgtgcacg aggggtgccc cgtcctcgag tgccttctcg atgtccgtca
     2161 ccacgctcat cggctcgtag ttgacctcga taaactccag ggcgtcctgc gccttgtagc
     2221 ggtcggtcgc caagacaacc gccaccggct caccggcgaa gatcacctca cccctggcca
     2281 ggggccagtg atccggg
//
LOCUS       DASTUV010000138         5110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1701251, whole genome shotgun sequence.
ACCESSION   DASTUV010000138 DASTUV010000000
VERSION     DASTUV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701251"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..471)
                     /gene="rplQ"
                     /locus_tag="VFT03_02860"
     CDS             complement(106..471)
                     /gene="rplQ"
                     /locus_tag="VFT03_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU4847115.1"
                     /translation="MRHARRGRKLGREAQHRKAMLGTMAGQVIQHGRIKTTAPKAKEL
                     RGVVDRLINTAKKDDLAARRQAVKVLKDKTVVRRLFEDVAPELDNRNSGYTRILKLGP
                     RPGDGAEIVYLELVNYTVE"
     gene            complement(458..1402)
                     /locus_tag="VFT03_02865"
     CDS             complement(458..1402)
                     /locus_tag="VFT03_02865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU4847116.1"
                     /translation="MLDIAPPRFRVEEEDERRGTFVAEPLPRGLGHTLGNSLRRVMLS
                     GLQGAAVTKVRIEGVSHEFSTIEGVKEDVVDIVLNVKGLKFRLEREEPIELEITKDGP
                     GEVTASDIQLKADVEVVDPETYIASVSEGGHLDMRLTVERGQGYARAEQNKSDADPIG
                     VIAIDSLFSPVQRVNYSVTETRAGARADLDSLTMEVFTDGRIEPREALQEASRQLIDI
                     LGLFTDGYQGTGAADSRQRGGRPVITDERPVEDLELTVRSYNCLKREGIDTIGQLATM
                     SEEELMNIRNLGMKSVDEIRSKLLEYGYDLASEGNEAR"
     gene            complement(1438..2040)
                     /gene="rpsD"
                     /locus_tag="VFT03_02870"
     CDS             complement(1438..2040)
                     /gene="rpsD"
                     /locus_tag="VFT03_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU4847117.1"
                     /translation="MARYTGPRGRRDRRAGVMLSSMRKNPLEKKPYPPGEHGRGRQRQ
                     TEYGMRLLEKQKARWYYGVSEKQFRRAYDRATRMQGVSGENLLRLMELRMDNVVYRMG
                     FATSRPQARQLVSHGHFLLNGRKHNIPSAILRQGDVITVKEKSRNLEPIVDAVENVVA
                     VPAWLEADHENFTGRVLHTPGRDEIDTPVEEQLIIEFYSR"
     gene            complement(2040..2447)
                     /gene="rpsK"
                     /locus_tag="VFT03_02875"
     CDS             complement(2040..2447)
                     /gene="rpsK"
                     /locus_tag="VFT03_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU4847118.1"
                     /translation="MGRQRQQRRGASRSRRRVRRNIPTGVVHIKSSFNNTIISVTDTE
                     GNVIAWESAGSLGFKGSRKSTPYAAQMTAESVANKAMDQGVRRVDIEVKGHGSGRDMA
                     ARTFQSVGIEVLSIKDVTGQPHNGCRPPKRRRG"
     gene            complement(2448..2819)
                     /gene="rpsM"
                     /locus_tag="VFT03_02880"
     CDS             complement(2448..2819)
                     /gene="rpsM"
                     /locus_tag="VFT03_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU4847119.1"
                     /translation="MARIAGVDLPREKRVEIGLTYEYGIGRSTARKILAETDVDPNTK
                     VRDLAEGEVGRIRTYVDQNVQVEGDLRREVNQNIRRLIDIGTYRGIRHRRGLPVRGQR
                     TKTNARQRKGPKPSIGGRKKR"
     gene            complement(2823..2936)
                     /gene="rpmJ"
                     /locus_tag="VFT03_02885"
     CDS             complement(2823..2936)
                     /gene="rpmJ"
                     /locus_tag="VFT03_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU4847120.1"
                     /translation="MKVRASVKPICERCKVIRRRGVVRVICTNPRHKQRQG"
     gene            complement(2950..3171)
                     /gene="infA"
                     /locus_tag="VFT03_02890"
     CDS             complement(2950..3171)
                     /gene="infA"
                     /locus_tag="VFT03_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU4847121.1"
                     /translation="MAKEDVIEVEGTVTEALPNTQFRVELESGHNVLAHISGKMRMNY
                     IRILPGDRVKVELSPYDLSRGRITYRFRS"
     gene            complement(3266..4030)
                     /gene="map"
                     /locus_tag="VFT03_02895"
     CDS             complement(3266..4030)
                     /gene="map"
                     /locus_tag="VFT03_02895"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU4847122.1"
                     /translation="MIVRKSQGELEAMREGGRITASCLRMLSGSVRPGVTTKELDSLA
                     EEFIHDHGGRPEFKGYQGFPASICASPNAMIVHGIPGPYRLKEGDIISLDVGVRYEGF
                     VTDSAITVAVGAVPLETTRLLETTRRALEAATEQARPGNHLGDIGHAIQSLAESRGYG
                     VVRDLVSHGVGRKMHEDPQIPNYGRPGTGPRLLSGMTFAIEPMITLGSYEIRLSEWDG
                     WSIYTADGSLAAHFEHTIAVTDEGPWVLTRNGGETG"
     gene            complement(4027..4662)
                     /locus_tag="VFT03_02900"
     CDS             complement(4027..4662)
                     /locus_tag="VFT03_02900"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU4847123.1"
                     /translation="MRIVLLGPQGAGKGTQAQRISRETGAKHISTGDLVRAEIKSGSE
                     LGQTIKDYNDRGDLVPDEIIVEMMKPYLGEADSWILDGFPRNESQAEALDRMLADIDA
                     ELDAAAALEAPDDLLVERLSGRRTSEATGNIYHAEHDPPPDDPDEDPGPFVQREDDRE
                     EAIRHRLKVYHDQTEPLKDYYADRGILVTVNATQDIPEVTQDILEAVEKKS"
     gene            complement(4659..>5110)
                     /locus_tag="VFT03_02905"
     CDS             complement(4659..>5110)
                     /locus_tag="VFT03_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEU4847124.1"
                     /translation="LGQLFAGGDPDSILGKLAAFFAPGNAPYLILYALLIVMFTYFYT
                     AVQFNPVEHADNLKKTGGYVPGIRPGQPTAVYLNNVLTRITLFGAVFLAIVAVFPYLI
                     TGILNLPQSIFLGGTSMLIVVGVSLDTVRQLESQLMMRNYEGFLRKR"
BASE COUNT          748 a         1713 c         1584 g         1065 t
ORIGIN      
        1 gaagctcgga gccggggtag gagaagacga ggttatcgca ggagacgacg gggggcccgc
       61 cgcggcgggc cccctcgttg ttgcggttct tcatgccgga cgtggttact ccacggtgta
      121 gttgaccagc tccaggtaga cgatctcggc gccgtcgccg ggcctggggc ccagcttgag
      181 gatgcgcgtg taacccgagt tgcggttgtc gagctccggc gctacgtcct cgaagagccg
      241 gcggactacg gtcttgtcct tgaggacctt gaccgcctgg cgccgggcgg cgaggtcgtc
      301 cttcttggcc gtgttgatga gcctgtccac gacgccgcgc agctccttgg ccttcggcgc
      361 ggtggtcttg atgcggccgt gctggatcac ctgtcccgcc atcgtgccca gcattgcctt
      421 acgatgctgc gcttcgcggc ccagtttgcg gcctctccta gcgtgcctca ttgccctcgc
      481 tcgcaaggtc gtagccgtac tcgagaagct tcgagcggat ctcgtccacg ctcttcatcc
      541 cgaggttgcg gatgttcatc agctcctctt cgctcatcgt cgcgagctgg ccgatggtgt
      601 cgatgccctc tcgcttgagg cagttgtagg agcggaccgt gagctccagg tcctcgaccg
      661 ggcgctcgtc ggtgatcaca ggccgaccac cgcgctggcg cgagtcggct gcccccgtgc
      721 cctggtagcc gtcggtgaag agcccgagga tgtctatgag ctgcctcgag gcctcctgca
      781 gggcctcccg cggctcgatg cgcccgtcgg tgaagacctc catggtaaga gagtcgaggt
      841 cggcgcgggc tccggcccgc gtctcggtga cggagtagtt cacgcgctgg accggcgaga
      901 acagcgagtc gatggcgata accccgatag ggtcagcgtc gctcttgttc tgctcggcgc
      961 gggcgtagcc ctggccccgc tcgaccgtga ggcgcatgtc gaggtgcccg ccctcggaga
     1021 cgctggctat gtaggtctcg gggtcaacca cctcgacgtc ggccttcagc tgtatgtcgg
     1081 atgccgtgac ctcacccggc ccgtccttgg tgatctcgag ttctatcggc tcctcgcgct
     1141 cgagcctgaa cttcaggccc ttcacgttga ggacgatgtc gaccacgtcc tccttgacgc
     1201 cctctattgt ggagaactcg tggctgacgc cctcaatgcg caccttggtg accgcggccc
     1261 cctggagccc cgagagcata acgcgcctca aggagttgcc caatgtgtgc cccaatcccc
     1321 ggggcagcgg ctcggccacg aaggtcccgc gccgctcgtc ttcctcttcc accctgaatc
     1381 ggggcggagc gatgtccaac atagtcaaag taccacctgc ttaataactg aagcgttcta
     1441 ccggctgtag aactcgatta tgagctgctc ctcgacgggc gtgtctatct cgtcccggcc
     1501 cggggtgtgc agcaccctgc ccgtgaagtt ctcgtggtcg gcctcgagcc aggcggggac
     1561 ggcgacgacg ttctctacgg cgtccactat aggctccagg ttccggctct tctccttcac
     1621 ggttatgacg tccccctgcc taaggatggc ggacggtatg ttgtgcttgc gcccgttcag
     1681 taggaagtgc ccgtgggaga cgagctggcg cgcctgcggc ctgctggtcg cgaagcccat
     1741 ccgatagacc acgttgtcca tcctgagctc catgagccgc agcaggttct cgccgctgac
     1801 cccctgcatc ctcgtagccc tgtcgtaggc ccgcctgaac tgcttctcgg agaccccgta
     1861 gtaccagcga gccttctgct tctcgaggag ccgcataccg tactctgtct gacgctgcct
     1921 tccgcgaccg tgctctcccg gcgggtaggg cttcttctcg agcgggttct tccgcatcga
     1981 ggagagcatc accccggccc tccggtctcg ccggcccctc gggcccgtgt aacgcgccac
     2041 tagcccctcc ttctcttcgg gggtcggcaa ccgttgtgcg gctgtcccgt tacgtccttt
     2101 atggagagca cctcgatgcc caccgactgg aaagtgcggg cagccatatc ccggcccgag
     2161 ccgtggccct tgacctcgat gtccacgcgc ctcacgccct ggtccatcgc cttgttcgcc
     2221 acgctctcgg ccgtcatctg ggccgcgtaa ggggtgctct ttcggctgcc cttgaacccc
     2281 agagagccgg ccgactccca ggctattacg ttgccctccg tatccgtgac cgagatgatc
     2341 gtgttgttga acgagctctt tatgtgcacc acgccggtag ggatgttcct gcggaccctc
     2401 cggcgcgagc gagaagctcc cctgcgctgc tgacgttgcc tgcccatcta cctcttcttc
     2461 ctcccgccta tggacggctt cggtcccttg cgctggcggg cgttggtctt cgtcctctgc
     2521 ccccgcacgg ggaggccacg gcggtgcctg atcccgcggt aggtccctat gtctatgagc
     2581 cgccggatgt tctggttcac ctcgcgccgc aggtcgccct cgacctgcac gttctgatct
     2641 acgtaggtcc ggatccgacc gacctcgccc tcggccagat ccctgacctt cgtgttaggg
     2701 tccacgtcgg tctcggccag gatcttcctc gctgtcgaac ggccgatccc gtactcgtag
     2761 gtcagcccga tctcgaccct cttctccctc ggcaggtcta cacccgctat gcgcgccata
     2821 tcctagccct gcctctgctt gtggcgcggg ttggtgcaga tgacccgtac taccccgcga
     2881 cgccttatta ccttgcaacg ctcgcagatt ggttttacgc tcgctcgtac cttcacaact
     2941 ctctccttct caactcctga acctgtaagt gatacgtccc cggctcaaat cgtaggggct
     3001 cagctctacc ttcaccctgt cgcccggcag tatccggatg tagttcatcc gcatcttgcc
     3061 cgagatgtgc gcgagcacgt tgtgcccgct ctcgagctct acccgaaact gcgtgttggg
     3121 cagcgcctcg gtgacggtgc cttcaacctc tatgacgtct tctttggcca aggacctccg
     3181 atctcaaatg gttacaagat ggctctaagg ccaaacggat atgttatcac gtcagcccct
     3241 gcactcacac cctcataccg cttgctcacc cggtttcgcc accgttccgg gtgagcaccc
     3301 acgggccttc gtccgtgacg gcgatggtgt gctcgaagtg ggcggcgagg gagccgtcgg
     3361 ctgtgtagat agaccagccg tcccactcgc tcaggcggat ctcgtagctc ccaagggtga
     3421 tcatgggctc aatggcgaag gtcatgcccg agaggaggcg cggccctgtg ccgggtctgc
     3481 catagttggg gatctggggg tcctcgtgca tcttgcgccc tactccgtgg gagacgaggt
     3541 ctctcaccac cccgtagccc cgactctcgg ctagcgactg tatggcgtgc cctatgtccc
     3601 cgaggtggtt gccggggcgt gcctgctccg tggccgcctc gagggcgcgg cgggtcgtct
     3661 ccaggagcct cgtcgtctct aaaggcaccg cgcccaccgc gaccgtgatg gcggagtcgg
     3721 tcacgaagcc ctcgtagcgg acgccgacgt cgagggagat gatgtccccc tccttcaggc
     3781 ggtagggacc ggggatgccg tgtacgatca tggcgttggg cgaggcgcag atcgaagccg
     3841 ggaagccctg gtagcccttg aactccggtc tgccgccgtg gtcgtggatg aactcttcgg
     3901 ccagggagtc gagctccttt gtcgtcacgc caggacggac gctccccgag agcatcctca
     3961 ggcaggaggc ggtgatacgg ccgccctcgc gcatcgcctc cagctcgccc tggctcttgc
     4021 ggacgatcac gacttcttct ccaccgcttc gaggatgtcc tgcgtgacct cggggatatc
     4081 ctgcgtcgcg ttcaccgtca ccaggatccc cctgtctgcg tagtagtcct tcagaggctc
     4141 cgtctggtca tggtagacct tgaggcggtg ccggatcgcc tcctccctgt cgtcctcgcg
     4201 ctggacgaat ggtccggggt cctcgtcggg atcatcgggc ggcgggtcgt gctcggcgtg
     4261 gtagatgttg cctgtggcct cgctagtgcg gcggcctgaa agacgctcga cgagcaggtc
     4321 gtccggggcc tccagcgcgg cggcagcgtc gagctctgcg tcgatgtccg cgagcatcct
     4381 gtccagggcc tcggcctggg actcgttgcg cgggaagccg tccaggatcc aggagtccgc
     4441 ctcgccgagg tagggcttca tcatctcgac gatgatctcg tcggggacca ggtcgccgcg
     4501 gtcgttgtag tccttgatgg tctggcccag ctccgaaccg ctcttgatct ccgcgcgcac
     4561 caggtctccg gtcgagatgt gcttcgcgcc ggtctcgcgc gagatccgct gcgcctgcgt
     4621 tcccttgcca gctccctggg gccccaatag cactatcctc atcgcttcct caagaaccct
     4681 tcgtagttac gcatcatcag ctggctctcg agctggcgca ccgtatccag ggagacgcct
     4741 accacgatca gcatcgaggt cccgccaagg aagatcgact gcggcaggtt caagatgccg
     4801 gtgatcaggt acgggaagac ggccacgatc gcgaggaaga cggcgccgaa gagcgtaatc
     4861 ctggtgagca cgttgttgag gtagaccgcg gtgggctggc ccggccgaat gccggggacg
     4921 tagccgcccg tcttcttcaa gttatcggcg tgctctacgg ggttgaactg caccgcggtg
     4981 tagaagtagg tgaacatcac gatgagcagc gcgtagagga tcaggtacgg cgcgttcccc
     5041 ggcgcgaaga acgccgcgag cttccccagg atagagtccg ggtcgcctcc tgcgaagagc
     5101 tggccgagaa
//
LOCUS       DASTUV010000139         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_57007, whole genome shotgun sequence.
ACCESSION   DASTUV010000139 DASTUV010000000
VERSION     DASTUV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57007"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1181)
                     /gene="ruvB"
                     /locus_tag="VFT03_02910"
     CDS             complement(126..1181)
                     /gene="ruvB"
                     /locus_tag="VFT03_02910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU4847125.1"
                     /translation="MEEMDDFTVRGEGVKEEEAPAEHPLDPEAFLEDDEPTLRPQNLH
                     EFVGQERLKDNLRIFVQAAKQRGEALDHILFAGPPGLGKTSLCRILATEMGVGLTTSS
                     GPSLERAGDMAAILTSLEEGDFLFIDEIHRLNRQIEEVLYPAMEDYAMDIVLGQGPSA
                     RTIRMDLPKFTLVGATTRTGLMTKPLLDRFGFSARLDYYAPEELEKIVVRNARILGIP
                     ISETGAKQLARRSRGTPRVANRLLKRVRDYAEVVGDGKIDEPTANAALEMQGVDILGL
                     DRTDRDYLGLIIDKFDGGPVGVGTLSVALGEARDTVEDVYEPYLLQCGLIQRTSRGRI
                     ATRRAYDHLGAPMPSGN"
     gene            complement(1184..1777)
                     /gene="ruvA"
                     /locus_tag="VFT03_02915"
     CDS             complement(1184..1777)
                     /gene="ruvA"
                     /locus_tag="VFT03_02915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU4847126.1"
                     /translation="MIHRLRGILVEKDTEGVVVDVGGVGYRASASVATLRALPSLGEE
                     CVIHTRMVVREDAMLLFGFAAPEERAAFDALTAVSKVGPKLALAVLSAMSPQQISEAV
                     ARGDVPKLSSVPGLGRKTAERLVLELKGKDLAVFGPEPVVSSDGGGGGPYMEAREALT
                     GLGYSLEEAEKALNSIPPQDSVEKYIKEALRRIGSRR"
     gene            complement(1802..2323)
                     /gene="ruvC"
                     /locus_tag="VFT03_02920"
     CDS             complement(1802..2323)
                     /gene="ruvC"
                     /locus_tag="VFT03_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU4847127.1"
                     /translation="MLTTSGRGQTILGIDPGTATMGWGVIRQEGNRLRYVQHGTVTTP
                     SNWEMPRRLGRLFDGVTELLEGYRPQTVAVEELFFNTNVTTAITVGQARGVAILAAYK
                     AGVEVHEYTPLQVKQAITSYGRADKRQVQEMVRALLNLREVPRPDDAADGLAIAITHA
                     FTSRMSGKVGVGK"
BASE COUNT          393 a          884 c          788 g          485 t
ORIGIN      
        1 gccagacctc ctctcgccgt cccgatcatc catctcgccc tgtatttacc acaagtgagg
       61 ccgactcaca aaaccggcat actctacgcc acgcgggagc agctagcgga gaagcgcgac
      121 ggattctagt tgccgctcgg catcggggcg ccgaggtggt cgtaggcgcg cctcgttgca
      181 atgcggccgc ggctggtgcg ttggatcagg ccgcactgca acaggtacgg ctcgtagacg
      241 tcctcgaccg tatcccgcgc ctcgcccaga gccacggaaa gcgtacccac tccgacagga
      301 ccgccatcga acttgtctat gatgaggccg aggtagtcgc ggtcggtgcg gtcgaggccc
      361 aggatgtcca ccccctgcat ctcgagcgca gcgttggcgg taggttcgtc gatcttgccg
      421 tcgcctacga cctcggcgta gtcgcgcacg cgcttgagga gccggttggc aacacgcggt
      481 gttccgcggc tgcggcgggc gagttgcttc gctccggtct ctgagatggg tatccccaga
      541 atcctggcgt tgcgcaccac tatcttctcc agctcctccg gcgcgtagta gtcgagcctg
      601 gcggagaacc cgaagcggtc gaggagcggc ttggtcatga gccctgtgcg cgtggtggcg
      661 cctaccaggg tgaacttcgg gaggtccatc ctgatggtcc gcgccgaggg gccctgcccg
      721 aggacgatgt ccatcgcgta gtcctccatc gcggggtaga ggacctcctc gatctgacgg
      781 ttcaggcggt ggatctcgtc tatgaaaagg aagtcgccct cctccagcga ggtgaggatg
      841 gccgccatgt cccccgcccg ctccaggctc ggcccgctcg aggtcgtgag gccgacgccc
      901 atctcggtcg ccaggatgcg gcagaggctc gtcttcccga gccccggagg gcctgcgaag
      961 aggatgtgat cgagggcctc cccccgctgc ttggcggcct gcacgaagat cctgaggttg
     1021 tccttgagcc tctcctgccc gacgaactcg tggaggttct gcggccggag cgtcggctcg
     1081 tcgtcctcga ggaaggcctc ggggtcgagc gggtgctcgg cgggagcttc ctcttccttc
     1141 acgccttcgc cgcggaccgt gaagtcgtcc atctcttcca tgctcaacgc ctgctcccta
     1201 tccgtcgtaa cgcctccttg atgtacttct cgacgctatc ctggggcggg atgctgttga
     1261 gcgccttctc ggcctcctcg agcgagtacc cgagccccgt gagcgcctcc cgcgcctcca
     1321 tgtacgggcc gccgcccccg ccgtcggagg agaccacggg ctcgggcccg aagaccgcga
     1381 ggtccttgcc cttgagttcc agcacgagcc gctcggcggt cttcctgccg agccccggca
     1441 cggaggagag cttgggcacg tcgccccttg ccaccgcctc ggagatctgc tgcggcgaca
     1501 tcgccgagag cacggcgagc gcgagcttgg gcccgacctt gctgaccgcg gtcagcgcgt
     1561 cgaaggccgc ccgctcttcg ggagcggcga agccgaaaag gagcatcgcg tcctccctca
     1621 cgaccatgcg cgtgtggatg acgcactctt cgccgagcga gggcagagcc cgcagcgtag
     1681 ccaccgaagc cgaagcccgg taacccactc ccccgacgtc cacgacgacg ccctccgtgt
     1741 ctttctcgac cagtatcccg cgaagtctat gtatcatcgg ctaattatca cgcacgaacg
     1801 gctatttccc cacccctacc ttccccgaca tcctcgaggt gaaggcatgg gttatggcga
     1861 tagcgaggcc gtcggcggcg tcgtctggcc gggggacctc tcgcaggttc aggagggcgc
     1921 gcaccatctc ctggacctgg cgcttgtcag cccgtccgta ggaggtgatc gcctgcttga
     1981 cctgcagcgg cgtgtactcg tgcacctcga cgccggcctt gtacgccgcc aggatcgcca
     2041 caccccgcgc ctgccccacg gtgatcgccg tggtcacgtt tgtgttgaag aagagctcct
     2101 cgacagccac agtctgtggc cgatagccct ccaacaactc cgtaactccg tcgaacaaac
     2161 ggcctaacct gcgcggcatc tcccagttgg acggcgtcgt cacggtccca tgctggacgt
     2221 agcgtagcct gtttccttct tgacggataa cgccccaccc catcgtagca gtccccgggt
     2281 ctatgccgag gatggtttgg cctctgccac tggtggtgag catagactaa ggttaccccg
     2341 tcctcgcctg gcttgcatcg gcttctacat gtagtgtacc gctctccatc ccggcactat
     2401 ggagccctga caccctggtg agtgttaccg aattggcttc agctccacgc cgagcgcctc
     2461 gagacgcctg tccgtgcgct ccatgagcca gtcgaggcgg gctacctccg ccctttgtct
     2521 tgcaggcatg tcttcgggcg tcagctcgtc
//
LOCUS       DASTUV010000140         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_485999, whole genome shotgun sequence.
ACCESSION   DASTUV010000140 DASTUV010000000
VERSION     DASTUV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485999"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFT03_02925"
     CDS             complement(<1..338)
                     /locus_tag="VFT03_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4847128.1"
                     /translation="MKFSVVIVNYASWPLTVRCVESLKASGYTGFEVVIVDNDRPEPP
                     EIGSPVRVIRNLGNAGVARAWNQGISASTGEIVVLINPDTVVGSDFFERVEAFFEENA
                     SAGVAGPRFL"
     gene            complement(335..1351)
                     /locus_tag="VFT03_02930"
     CDS             complement(335..1351)
                     /locus_tag="VFT03_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4847129.1"
                     /translation="MAFRVTVVIPNWNGERFLRLCLDSLRRQTFPDFESVVVDNGSSD
                     GSLSLVKRDFPEVRALALGKNRGFSAAVNAGIGASGAELVALLNNDTEQDPGWLAALV
                     RAADEHPEVGFFSSKLVDFHDRRLLDGAGDALRLSGLPYRLGHGERDRGQFDTPSYVF
                     SACGAAALYRRAMLDDVGLFDEDFVSYCEDGDLSFRAQLAGYHCLYVPDAVVYHVGSV
                     STGGKRSATATRLGTRNSLSLLVKNLPLSAVPHVLPFFVLGQLARLLTAAATGSLGAH
                     LRGLAESWHHLPIMLEKRRRIQKNRRTSDVKIRSLLRGSSLAATASIARRLRDRLEER
                     LVHR"
     gene            complement(1396..3060)
                     /locus_tag="VFT03_02935"
     CDS             complement(1396..3060)
                     /locus_tag="VFT03_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4847130.1"
                     /translation="MRAAVSLKGIGKKYRVTPSRSLRLKEVLSFGKVKRSHDFWALQD
                     INLEVEPGTTLGILGRNGAGKSTLLKIISGVLQPTTGTAEVNGRLTAIFSLGSGFNPE
                     FTGRENAMLNGLILGIDNQEMLERFDDIAAFADIGEFMDRPIKTYSSGMRSRLGFAVA
                     VNVDPDILVLDEALSAGDAAFKKKALQRMYDLRDSGTTVLFVSHSMGMVKQFCTDAIL
                     LHKGRLVSSGSPNEVADHYKELLENALQKDSQTDGTDRGLDDMLDSEEEEDLEGVGSR
                     DGSARPGGGARGAGIRTVEVLDEKGEPVSTVRSGSTVTVRVHARYAEAAEESALGITL
                     QSRKAGVAVFSTDTNREGTSLGPREASEEATVDFTFEVPLQPGTFTVDAAVSVPQGEG
                     SYLNQAKEAASFRVTRTGEPPSRPKEARPRTRPNWSELSIGGLVDLPTKVEVHSPEGE
                     RERPRKTVPEPDGAQGATEAAERRAEALDVDLESVEPTGADAQVVVEDVEHAAEEEGK
                     VHATGAAERKAKELGVDLRSVEGTGKYGRITVGDVDKAAKAQASDG"
     gene            complement(3057..>3855)
                     /locus_tag="VFT03_02940"
     CDS             complement(3057..>3855)
                     /locus_tag="VFT03_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847131.1"
                     /translation="FDSLATFWRKRWLLRYFVHRQVTRKYRKSYLGFAWAFLSPLIWV
                     FFLTLIFSEIVGLRFRVVEGDPTLNFGLYLYCGLLPFLAFSEAANKGLNAITGNKGLV
                     QQVVFPLELLPLTNVITSMIDKVFGLGALLVVLLVFGRELHWTISLLPLIFVLQLVFI
                     LGLSYFMAILGTYLPDVGEMTRPVIRGMFFITPIIWTADRLPENLRWVVDYNPLAYLV
                     ESYRDLILNGELPGEMATLYFTLFSVALFIVGFALFVRLKKGFADLM"
BASE COUNT          683 a         1296 c         1181 g          695 t
ORIGIN      
        1 tccaggaacc tgggtcccgc gacccctgcc gaagcgttct cctcgaagaa cgcctccacc
       61 ctctcgaaga aatcgcttcc cacgaccgtg tccgggttta tgaggacgac tatctcccca
      121 gttgaggcgg agatcccctg gttccacgcc cgcgccaccc ccgcgtttcc gaggttccgg
      181 attacacgta ccgggctccc gatctccggc ggctccggac ggtcgttgtc cactatgacg
      241 acctcgaagc cggtgtagcc cgacgccttc agcgattcaa cgcagcgcac tgtgaggggc
      301 caggaggcgt aattgacgat gacgacggag aacttcaccg gtgtaccagg cgctcctcga
      361 gccggtcgcg cagcctgcgc gcgatgctag ccgtggccgc gagcgacgat cccctgagga
      421 ggctccggat cttcacgtcc gaggtcctcc tgttcttctg gatccggcgc cgcttctcga
      481 gcatgatcgg caggtgatgc cacgattcgg caagcccccg caggtgcgcc cccaaagagc
      541 ccgtcgcggc tgcggtgagc aaccgcgcga gctgcccgag gacaaagaac ggcagcacgt
      601 gcggcaccgc ggagaggggc aggttcttta ccaacaagct cagggagttt cgggtaccca
      661 gccgggtcgc ggtcgcgctc ctcttgccgc ccgtggaaac cgaccccaca tggtagacga
      721 cagcgtcggg gacgtagagg caatggtagc ccgcgagctg ggcgcgaaag ctcagatccc
      781 cgtcctcgca gtaggagacg aagtcctcgt caaagagtcc gacgtcgtcc agcatcgccc
      841 ggcggtacag cgccgccgcc ccgcacgccg agaagacgta gcttggcgtg tcgaactggc
      901 cgcggtcccg ctctccgtgc ccgaggcggt aggggagacc gctcaacctg agggcgtccc
      961 ctgccccgtc caggagcctc ctgtcgtgga agtccaccag cttgctagag aaaaatccca
     1021 cctccggatg ctcgtccgcc gcgcgcacca gcgccgctag ccagcccggg tcctgctccg
     1081 tgtcgttgtt taggagcgcc acgagctccg ctcctgaggc tccaatccca gcgttcactg
     1141 ccgcggagaa gccccggttc ttcccgagcg cgagggcccg cacctctggg aagtcgcgct
     1201 tgaccaggct caacgagccg tccgaggagc cgttgtctac cacaacgctc tcgaagtccg
     1261 gaaaagtctg tcggcgcagg gagtccaggc agagcctgag gaaacgttca ccgttccagt
     1321 tggggatgac gactgttacg cggaaagcca aggcttcagg ccgcccgctt gagcgtgacc
     1381 tccggcgcac gccgcttagc cgtcgcttgc ctgcgctttt gccgctttgt ccacgtcacc
     1441 gacggttatg cgaccgtact tgcccgtgcc ctcgacgctt ctgaggtcaa cgccaagctc
     1501 tttggctttg cgctcggcgg ccccggtggc gtggaccttg ccctcctctt cggcggcgtg
     1561 ctccacgtct tcgaccacga cctgggcatc ggcgccggtg ggctcaaccg actccaggtc
     1621 cacgtccagc gcctccgccc ggcgctcggc ggcctcggta gccccttgcg ccccgtctgg
     1681 ctccggaacg gtctttctag gcctctctcg ctcgccttcg gggctgtgga cctcgacctt
     1741 cgtgggcaga tcgaccaggc caccgataga gagctcgctc cagttcgggc gagtacgcgg
     1801 tcgggcttct ttcggccgag aggggggttc accggttcgg gtgaccctga aggatgccgc
     1861 ctccttagcc tggttcaggt acgagccctc accctgggga acggagacgg cggcgtctac
     1921 agtaaaggtg cccggttgca gcggcacctc gaaggtgaag tctacggtcg cctcttcact
     1981 agcctccctg ggtccgagcg aggttccttc ccggttagtg tcagtcgaga acacggccac
     2041 cccggccttc ctgctttgca gggtaatgcc cagggcgctc tcctccgcgg cctccgcgta
     2101 gcgcgcgtgc acccggacgg ttaccgtgga ccccgagcgc acggtgctga ccggctcccc
     2161 cttctcgtcg agcacctcta cggtccggat cccggctcct ctcgcacccc cgccagggcg
     2221 tgcggagccg tccctggagc ccacaccctc gagatcctct tcctcctcac tgtcgagcat
     2281 gtcgtcaagc ccacggtctg tgccgtcggt ctggctatcc ttctgcaggg cgttctcgag
     2341 cagctccttg tagtggtccg ccacctcatt ggggctgccg gagctcacca ggcgtccctt
     2401 gtggagcagt atggcgtccg tgcagaactg cttgaccatc cccatgctgt gcgagacgaa
     2461 gaggacggtg gtgccggagt cgcgcagatc gtacatccgc tgtagagcct tcttcttgaa
     2521 cgccgcgtcg ccggcggaga gggcctcgtc gaggaccagg atgtcgggat ccacgttcac
     2581 cgcgacggcg aagcccaggc gcgagcgcat cccgctcgag tacgtcttga tcggccggtc
     2641 catgaactct ccgatgtccg cgaaggccgc tatgtcgtcg aaccgctcca gcatctcctg
     2701 attgtctatt cccaggatca ggccgttcag catggcgttc tcgcggcccg tgaactctgg
     2761 gttgaagccc gaccccaggc tgaagatcgc cgtgaggcgg ccgttgacct cggccgtgcc
     2821 ggtcgtaggc tgcaagacgc cggagattat cttgaggagg gtgctcttgc cggccccgtt
     2881 gcggcccagg atgcccaggg tcgtgcccgg ttccacctcc aggtttatgt cctgcagcgc
     2941 ccagaagtcg tggctgcgct tgaccttgcc gaagctcaag acctctttca gccgcaggga
     3001 gcgactcggg gtgacccggt acttcttccc tattcccttg agggatacgg cggccctcac
     3061 atcagatccg caaacccttt cttgagccgg acgaacagcg cgaaacccac gatgaacagc
     3121 gcgaccgaga agagcgtgaa gtagagcgtt gccatctcgc ccggcagctc gccgttcagg
     3181 atcaggtccc ggtaggactc gaccaggtag gccagaggat tgtagtcgac cacccaccgg
     3241 aggttctccg gtaacctatc cgctgtccag atgataggcg tgatgaagaa catcccccgt
     3301 atgacgggac gcgtcatctc gcccacgtcg ggaagatagg tgccgaggat agccatgaag
     3361 tagcttagac ccagaatgaa caccagctgg agcacaaaga tgagcggcag tagcgagatc
     3421 gtccaatgca gctctcgccc gaacaccagc aaaacgacca gcagcgcccc caggccgaag
     3481 accttgtcga tcatcgacgt gatcacgttg gttaaaggta acaactcgag cgggaagacc
     3541 acctgttgca ccaggccttt gttgcccgtg atggcgttca ggcccttgtt cgccgcttcg
     3601 gagaaggcca ggaaagggag caggccacag tacaggtaga ggccgaagtt gagcgtggga
     3661 tctccctcga ccaccctgaa cctcagcccg actatctccg agaagatgag ggtaaggaag
     3721 aagacccaga tcaggggcga caggaaggcc cacgcgaaac ctagataaga cttccggtat
     3781 ttgcgagtca cctggcggtg tacgaagtac ctcagcagcc agcgcttgcg ccagaacgtc
     3841 gccagagaat cgaag
//
LOCUS       DASTUV010000141         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_560777, whole genome shotgun sequence.
ACCESSION   DASTUV010000141 DASTUV010000000
VERSION     DASTUV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560777"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..2501)
                     /locus_tag="VFT03_02945"
     CDS             complement(12..2501)
                     /locus_tag="VFT03_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4847132.1"
                     /translation="MSDAADNAFSHLVVIGSSAGGAEAVSRVLSHTRNLDEAAPEILR
                     IIGEHLGWEAGVFWTVDDGSLRCARTWSAASAPPGPLAQQCQGARFARGEGLPGRVWT
                     RGEALWVEDALEGEDPLLRVLAGEGLRGAIAFPVQDGGILGVFEFFRREVLSLDEQLL
                     QTASIIGGQIGQFVERRRAEEERDRYLLRERRARREAIDILESINDAFFALDRDWCLT
                     YVNQKAEQFWSRTRTDLLRKNLWEEFPEVAESEISREIRHAMENGATTSFETIWPVIG
                     VWVAGRAYPSSGGVSVYLQDITDRKQAERELLTLKDDLDAELSAMSGLHHLSTRLLAA
                     SELHTVLEEVLDASIELQGADLGNIQLYDRETGTLEIVAHRGFDQEFLDYFSDTDENA
                     ACGRAMKQGERVVIEDVEKDARFEPHRRIAASAGFRAVQSTPLFDRTGELLGMLSTHF
                     RQPHRSSERELRMTDLYARQAAEMIGAKLAEERLRENEEWLQLAARAARSGTWEWDLA
                     SGEIRWSDEHRELFGIASEKPVTREKWFAMVHPDDLPAIEEAGLRCSEEGEEWPEVIE
                     YRIRRGGETRWICAWGKTVRNDSGRPVRILGISVDATDHKKAEEERERLIAQELAARA
                     QAEERRRLSRELHDRVAHDIALVHQSLELHQALKEHDPEKSAAKLELARKSTREALES
                     TRNLSMELRRLEVSRGLESALSDLLRDVIPPSASFEVSAEGDEAPVPAEVRTQVLLIL
                     REAIRNAVTHSDCSRIKVRLQVLPEAMVCSVEDDGQGFHTHDLHPADHNGLKSMEERA
                     SLLGGTLGVSSTPGRGTTIQVTVPLGERDGE"
BASE COUNT          484 a          983 c          904 g          532 t
ORIGIN      
        1 ttatgcgacc gtcattctcc atcgcgctca cccaatggta cggtcacctg gatcgtcgtg
       61 cccctccccg gggttgagga gacgccgagg gtccctccta gcagcgaggc gcgttcctcc
      121 attgacttca ggccgttgtg atctgccggg tgtagatcat gagtgtggaa accctggccg
      181 tcgtcttcga cagagcacac catcgcttcc ggcaggactt gcagccgcac cttaatgcgg
      241 ctgcaatccg agtgtgtcac ggcgttgcgg atcgcctcgc gcaggatcag taacacctgg
      301 gtgcgaacct cggccggcac cggcgcctcg tcgccttccg ccgagacctc gaagctggcg
      361 ctcgggggga tcacgtcgcg caggagatcg gagagggccg attccagacc ccggctgacc
      421 tccaggcgac ggagctccat ggagaggttt ctggtggatt cgagggcctc cctggtcgac
      481 ttcctggcca gctcgagctt ggccgcggac ttctcggggt cgtgctcttt gagagcctgg
      541 tgcagttcca ggctctggtg cacgagggca atatcgtggg ccacgcggtc gtgcaactcg
      601 cgggataggc ggcggcgctc ctcggcctgg gcccgggccg ccagctcctg ggcgatgagc
      661 cgctcgcgct cctcctcggc ctttttgtgg tcggtggcgt ccacggagat acccaggatc
      721 cggaccgggc ggccactatc gttcctgacg gtcttacccc aggcgcagat ccaacgtgtc
      781 tcgccgccgc gccggatgcg gtactcgatg acctcgggcc actcttcgcc ctcctcggag
      841 caccgcaggc cagcctcctc gatggctggc aggtcatcag ggtggaccat ggcaaaccac
      901 ttctcgcgcg taaccggctt ctcggaggcg atgccgaaga gctcgcggtg ctcgtcggac
      961 cagcggatct cgccgctcgc cagatcccac tcccacgtcc ccgaccgggc cgcccgcgcg
     1021 gcgagctgca accattcctc gttctcgcgc aatctttcct cggccagctt agccccgatc
     1081 atctcggcgg cctgtcttgc gtaaaggtcg gtcatccgca actcgcgctc agaagacctg
     1141 tgcggctggc gaaagtgcgt cgagagcatg ccgagcagtt caccggtgcg gtcaaagagc
     1201 ggcgtggatt ggacggcgcg gaacccggcg gaggcggcga tccgccggtg cggctcgaat
     1261 cgagcatcct tctcgacatc ttcgatgacg acccgttcgc cctgcttcat tgcccggcca
     1321 caagcggcgt tctcatccgt gtcgctgaaa tagtctaaga actcctggtc gaagccacgg
     1381 tgcgcgacga tctccagcgt gccggtctct cgatcataga gctggatgtt gccgaggtcg
     1441 gcgccttgca actcgatgga ggcatcgagg acttcttcga gcaccgtatg aagctcggag
     1501 gcggccagaa gcctagtgct caagtggtgc aggccgctca tcgcggagag ctcggcatcc
     1561 aggtcgtcct tcagagtcag caattccctc tccgcctgct tgcgatcggt aatgtcctgg
     1621 aggtacaccg agacgccccc ggaggatggg taagcgcggc cggcgaccca gactcctatg
     1681 accggccaga tcgtctcgaa gctcgtggtc gcgccattct ccattgcgtg acggatctca
     1741 cgggaaatct cggactcggc tacctcgggg aactcctccc agaggttctt cctcaacaga
     1801 tccgtcctcg ttctggacca gaactgctcg gctttctggt tgacgtaggt caggcaccaa
     1861 tcccggtcca gcgcgaagaa ggcgtcgttt atgctctcca ggatatcgat tgcctctctc
     1921 cgcgcccgcc gctcgcgcag gaggtagcgg tcgcgctctt cctccgcgcg ccggcgctcg
     1981 acgaactggc cgatctggcc cccgatgatg gaggcggtct gcaacagctg ctcgtccaga
     2041 ctcaggacct cacgccggaa gaactcgaag acgccgagga tacccccgtc ctggacagga
     2101 aaggcgatgg caccgcgcag gccctccccg gccaatacgc gcaatagagg atcctctccc
     2161 tccagggcat cctccaccca cagcgcttcg cccctcgtcc agacccggcc cgggagcccc
     2221 tcgccgcgcg cgaagcgcgc cccctggcac tgctgggcca gggggccggg cggagcgctg
     2281 gccgcgctcc aggtcctcgc gcagcgaaga ctaccatcgt caaccgtcca gaaaacgccg
     2341 gcttcccacc cgaggtgttc gccgattatc cgcaagatct ccggggccgc ctcgtccagg
     2401 ttacgggtat ggctgaggac gcgactcacg gcttccgccc ctccggcgga gctaccgata
     2461 accaccaggt gcgagaacgc gttgtcggcg gcgtcggaca atactgttcc cctaacgtgc
     2521 gctactagtc aattataatg tgaagcgaac ggcaggtcga gccgatctcg aggccagcct
     2581 cgtataccgt gcggcgacgg cgggtatatt actagctcct ttgctggtcg tcgcgtggct
     2641 tgcgtacgca cgtaaaccga cacgatggtg atcgggacct cctcgggcgg ggtggaggct
     2701 cttcgcaggc tggccgggga cttgccgccc gacctgcctg cgggcacgcc ttcaccgccg
     2761 aaagcatgct ctacgataag tatgacgagg tggagaaggc gctgtacatt gcgctcaaca
     2821 cgctcgaaga aagcgcgctg atggctgaca ggctggccgc ccgctcgcac gagcaccagc
     2881 acggtcacgc ggcgacgcgc ttc
//
LOCUS       DASTUV010000142         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_879232, whole genome shotgun sequence.
ACCESSION   DASTUV010000142 DASTUV010000000
VERSION     DASTUV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879232"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..2053
                     /locus_tag="VFT03_02950"
     CDS             392..2053
                     /locus_tag="VFT03_02950"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4847133.1"
                     /translation="MRTAIAPHELLPKGLQLESLSIETGRVSICVSSGTRKSVCPLCG
                     RGSFRVHSRYLRTVSDLPWHGISVELEVRARRFFCDESSCERKIFCERLEKVAVRARK
                     TSRLEEALLTIALELGGRAGARLALALGIVAARDTLLRRIKGAPLPEVGKLRVLGVDD
                     FAFKKGSTYGTILVNLEDHKVVDLLAERSQESLVAWFRSHPAAEVEVATRDRSKIYRE
                     GLAKGAPGATHVADRWHLLHNLTLTLEEYLLQKRPVLREAAAPEAAPEEKDDAEEFAS
                     GPIMPNRPRTHDRKIEEAARKRHERLVEQWRNIRRLYLAKADLRDICRQLGISARTVY
                     RYKDLTEPPPRPAYKRKKSVLDLYVPYLVRRWKEGCHNGKRLYREIREQGYSNSEEIC
                     ARFTAQLRRAEARGKPISSVPRAKAGSAVGLSPTAKNVAALFMRHEEKLDEEQEEYLG
                     RLCEADEALADTRRLTQEFACMVRRLEGEDLDGWLKDAEESRSTAMRSFALGLRKDLH
                     AVRAGLTEKWSNGPVEGFVHKLKLLKRQGYGRAGFELLRARMLAA"
BASE COUNT          525 a          776 c          821 g          426 t
ORIGIN      
        1 cgtggtcatc ggccagggca cgcggctgtt ccccgacacc ggcccggacc gagcgctcgt
       61 cctggtcgac tcccgagcca ccacgaacgg cgtaacgatc caggtctacc ggcccatcgg
      121 gcgcccgcag tacgaaacgg ccacggccga cctgaacacg tgagatagat acgtcatcga
      181 gggagccgga cgaccgccga ccggaccggc ctactcgtca ttctccccct cgccccgcgc
      241 gtcttcgccg cgggtgggga aggatgccac aatccggtcc taacggccag tctatgtccc
      301 gctatgcgtc ccagactttg gttctgtcgc gttcctggtg aataggcctt accgttaggt
      361 tccgtcgagc cgggtaagga gtagtgccac catgaggacc gccatcgcac cccatgaact
      421 gttgccgaag ggtctgcaac tcgaaagcct cagcatcgag accgggcgcg tgagcatctg
      481 cgtatcgtcc ggcacgagga aaagcgtctg cccgctctgt ggtcgcggct cctttcgggt
      541 gcacagtcgc taccttcgca ccgtctccga cctgccctgg cacgggatct cggtcgagct
      601 cgaggtacgc gcgaggcgct tcttctgcga cgagagttcg tgcgagcgaa agatcttctg
      661 tgagaggctt gagaaggtcg ccgtccgcgc ccgtaagacc tcccggctag aggaggccct
      721 gctgacgatc gccctcgagc taggcggcag ggcgggcgcc aggctggccc ttgcgctggg
      781 tatcgtcgcc gctcgtgaca ccttgctaag gaggatcaag ggcgcgccgc tgccggaggt
      841 cgggaagctg agggttctag gcgtggacga cttcgctttc aagaaaggca gcacgtatgg
      901 cacgatcctc gtaaacctcg aagaccataa ggtcgtggac ctgcttgcgg agcgctccca
      961 ggagagcctg gtggcctggt tcagaagcca ccccgccgcg gaggtggagg tggccacccg
     1021 cgaccgttcg aagatctacc gggaggggtt ggccaaaggc gcccccggtg ccacacacgt
     1081 ggcggaccgc tggcacctgc tccacaacct cacgctcacg ctggaagaat acctgctcca
     1141 gaagcggccc gttctgcgcg aggcggccgc gccggaagcg gcacccgagg agaaagacga
     1201 cgccgaggag ttcgcttccg ggccgatcat gcccaatcgt ccgagaaccc acgatcggaa
     1261 gatcgaggag gcggcgcgca agagacacga acgcctcgtc gagcagtgga ggaacatccg
     1321 tcgcctctac ctcgcgaaag ccgatctcag ggatatctgc cgccagcttg gcatcagcgc
     1381 caggaccgtc taccgctaca aggacctcac cgagccgcca ccgcgtccgg cctacaagag
     1441 gaagaagagc gtcctcgacc tctacgtgcc gtatctcgtg agacgctgga aggagggatg
     1501 ccacaacggc aagcggctct accgggagat ccgggaacag ggctactcaa acagcgagga
     1561 gatctgcgcc cgtttcaccg cccaactccg tcgcgccgaa gccaggggaa agccgatctc
     1621 ctccgtgccc cgagcgaagg caggctccgc cgtagggctt tctcccacgg ccaagaacgt
     1681 ggccgctttg ttcatgcgcc acgaagagaa gctcgacgag gaacaggagg aatatctggg
     1741 aaggctttgc gaggctgacg aggccctcgc cgacacgcgt aggttaaccc aagagttcgc
     1801 ctgtatggta agaaggcttg aaggtgaaga cttggacggg tggctaaagg acgccgagga
     1861 gtcacggtcc accgcgatgc gcagtttcgc cttaggcttg aggaaggacc tccacgcggt
     1921 gcgggcggga ctcacggaaa agtggagtaa cggacccgtg gaaggcttcg tgcacaagct
     1981 caaattgctg aagcgacagg gctacggacg ggcggggttc gagttgctcc gggcgaggat
     2041 gctggcggcc tgagggcagg cggcgatcgg ttcggggagg agatacttcg tttcatcaag
     2101 aagccgacag aacccagggt tcatggcgag gtttgcccca aatacccccg atccgtggca
     2161 gcaatagtgg cagcaataga gccaaaaccg gcgcgaacga cggaaaacgc ccaaagacga
     2221 attccgctta tctatgcggt ttccttgaca tcgggtaaca cccagaaagg tattatgggg
     2281 gattagaagt ccgctgctct gtcccctgag ctacgggagc attcggctta tctatgcggg
     2341 attctcgact tcggggtcaa gctagaaccc caatggtagc aatgcggcag cagcgcacga
     2401 aacgcccctt cacagcgtat ggtcaattgc gtcggccagg ccaccgtcca tacccacgat
     2461 gacgtgggag caggtgtcca ggttgatgga gaggctgacg tgaacgtatt tcgggtgctt
     2521 gtctgccatg aggagtattg tagcgcag
//
LOCUS       DASTUV010000143         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_771863, whole genome shotgun sequence.
ACCESSION   DASTUV010000143 DASTUV010000000
VERSION     DASTUV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771863"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="gltX"
                     /locus_tag="VFT03_02955"
     CDS             complement(<1..1009)
                     /gene="gltX"
                     /locus_tag="VFT03_02955"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU4847134.1"
                     /translation="MENDARIRVRYAPSPTGRLHVGGVRTALFNWLFARKNGGTFVLR
                     IEDTDLERSTEESVEQLGRSMRWIGLDWDEGPGAGGPHPPYRQTERFDLYREAAKKLL
                     DSGAAYYDFATPEELAWLRERARAEGRQPIYTGGEYREMDPDEAGERIQAGEPYTVRF
                     KTPREGQTVVQDMIRGPVTFENANLEDFVLMKSTDTPTYNFAAAFDDAEMEISHVIRG
                     DDHLSNTPRQILIHEALGNPLPAFAHVPQVLGPDKKKLSKRHGAASVEDFAERGYLPE
                     ALFNYLALLGAGYAADEEIFTPDELAERFRIDRVSGNPAILDEKKLRAVNAVYMRRKS
                     LE"
     gene            complement(1140..1796)
                     /locus_tag="VFT03_02960"
     CDS             complement(1140..1796)
                     /locus_tag="VFT03_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEU4847135.1"
                     /translation="MATAKETILWRIKHATRDVPEAERPEDVSVEREYRKEDGAEREE
                     IVARFAERTAEYEADVRRVGHEDLPGAVEEALKRRGVRRLVIPPYLPREWIPDGIETL
                     RDATRPRLSNQKLDESDGVLTGCALGIAQTGTIVLDAGRAQGRRALTLLPDYHLCVVR
                     EQQIVGLVPEAFASLEKAVKGEGRAITFISGPSATSDIELDRVEGVHGPRTLEVLIVG
                     "
     gene            complement(1796..>2015)
                     /locus_tag="VFT03_02965"
     CDS             complement(1796..>2015)
                     /locus_tag="VFT03_02965"
                     /inference="COORDINATES: protein motif:HMM:NF023298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HEU4847136.1"
                     /translation="NVAMQTVARVFSDPQFYEKAQRMARVGQWPFARGGTIGRLPGRL
                     AGWTAVRDLKPIPQQTFREWWKRNRGSV"
BASE COUNT          305 a          653 c          650 g          407 t
ORIGIN      
        1 cctccagact cttgcgccgc atgtagacag cattcacagc tctcagcttc ttctcgtcga
       61 ggatagccgg gttgccgctt acacggtcga tgcggaagcg ctcggcgagc tcgtccggag
      121 tgaagatctc ctcatcagcg gcgtagccgg cgccgaggag cgccaggtag ttgaagagcg
      181 cctcgggcag gtagccccgc tccgcgaagt cctcgacgct cgctgcgccg tggcgcttgc
      241 tgagtttctt cttgtctggc cccaggacct gggggacgtg ggcgaaggca gggagcgggt
      301 tgcctagggc ctcgtggatc aggatctggc gcggcgtgtt cgagaggtgg tcgtcacccc
      361 ggatgacgtg gcttatctcc atctccgcgt cgtcgaaggc ggcagcgaag ttgtaggtcg
      421 gggtgtctgt ggacttcata agcacgaagt cctccaggtt ggcgttctcg aaggtgactg
      481 ggccacggat catgtcctgc acaacggtct ggccctcgcg cggggtcttg aaacggacgg
      541 tgtagggctc gcccgcctgt attctctctc ccgcctcatc tgggtccatc tcgcggtact
      601 cgccgccggt gtagatcggc tgccgacctt cggcccgcgc cctttcgcga agccaggcga
      661 gttcctcagg cgttgcgaag tcgtagtaag ccgcgcccga gtcgaggagc ttctttgcgg
      721 cctcgcggta gaggtcgaag cgctcggtct gacggtatgg gggatgtggg ccgccggccc
      781 ctgggccctc gtcccagtcg aggccgatcc aacgcatcga gcgcccgagc tgctcgaccg
      841 attcctccgt ggagcgctcc agatccgtat cctctatccg cagcacgaac gtgccgccgt
      901 tcttgcgcgc gaagagccag ttgaagagcg ccgtgcgtac accaccaacg tggagcctgc
      961 cggtcggact cggcgcgtac ctgaccctga tccttgcgtc gttctccata gtctaaacct
     1021 aacacacgtt agtggaggcc gtgcgccctc gacggtgttt tccctggttt ggcacgactc
     1081 ggaggtaacc acacagccgt ccttccggat gaaaatggcc caaagtatgt agacctgcgt
     1141 cagccgacta tcagcacctc gagggtacgc gggccgtgca cgccctcgac cctgtcgagc
     1201 tcgatgtccg aggtcgccga cgggcctgag ataaaggtga tggccctgcc ttcgcccttg
     1261 accgcctttt ccaggctggc gaaggcctcg ggtactaggc ccactatctg ctgctcgcgc
     1321 acgacgcaca ggtggtagtc ggggagtaac gtgagcgccc ggcggccctg cgccctgccg
     1381 gcatcgagca cgatggtgcc cgtctgggct atgccgaggg cgcagcccgt cagcacgccg
     1441 tcgctctcat cgagcttctg gttcgagagc cggggacggg tagcgtcacg aagggtctct
     1501 atgccatctg ggatccactc tctaggcagg taagggggga tgacgaggcg tctcacgccg
     1561 cgccgcttca gggcttcttc tacggctccg gggagatcct cgtggcccac gcgacggacg
     1621 tctgcctcgt actccgccgt gcgctcggcg aagcgcgcga cgatctcctc gcgctccgcg
     1681 ccgtcttcct tgcggtattc gcgttcgacg gatacgtctt cgggacgctc cgcctctgga
     1741 acgtcgcggg tggcatgctt tatgcgccac aggatcgtct ctttcgcggt cgccactaga
     1801 ctgaccccct gttccgcttc caccactctc ggaaggtctg ctgtggaata ggcttgaggt
     1861 cgcgtacggc ggtccaaccg gcgagcctgc ctggaaggcg tccaatagtg cccccgcggg
     1921 cgaagggcca ctggcctact ctcgccatcc tctgggcctt ttcgtagaac tgtgggtcgg
     1981 agaagactct ggcgaccgtc tgcatcgcga cgttc
//
LOCUS       DASTUV010000144         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1274567, whole genome shotgun sequence.
ACCESSION   DASTUV010000144 DASTUV010000000
VERSION     DASTUV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274567"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFT03_02970"
     CDS             <1..493
                     /locus_tag="VFT03_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU4847137.1"
                     /translation="ANIMKYTDGLFRDVFFEVAKEYENDFEEIDDRIVDNMCMQLVMK
                     PNLYDVLVCPNLYGDILSDLCAGLTGGLGVAPGANIGEEIAVFEPVHGSAPKYAGQNR
                     ANPLATLLSAKNMLYHLGYNEEGDRMQRGIEAALASPETRTKDLGGSAGTKEFTQAIV
                     ANL"
     gene            568..1641
                     /locus_tag="VFT03_02975"
     CDS             568..1641
                     /locus_tag="VFT03_02975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU4847138.1"
                     /translation="MTRLRYTIEELAKMGDDMLVVAPRYPDGGPDSYAGAPIYRVPGV
                     PFPPYPRIKLSFAHPGVGRALWRFGPDLIHAVNPFILGPGGIYYSRRLEVPLVASYHT
                     NVAAYARYYNLNFSYNAARWWTRQLHNRAEINLCTSATTMDYLLKEGVKRIRLWPQGV
                     DARRFHPEKASQVWRERLSDGHASDRLLLYVGRLAPEKGIERLKAILTGVPGTRLAIV
                     GDGPARADLEREFSGTAAVFTGVLQGEDLASVYASADAFLFPSTTDTLGLAMLEALAS
                     GLPVIAARGGASHEIVSDEENGLLYEATSTPSLVSAVRRLFSNEGFRAALARRARETA
                     EKRDWESSTHTLHGYYEEAMGRR"
     gene            1638..2105
                     /locus_tag="VFT03_02980"
     CDS             1638..2105
                     /locus_tag="VFT03_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU4847139.1"
                     /translation="MSTKGTRLKRGGVRFSKFTVVGLSNAAVDIGTLNLFLWLAPTRD
                     PSVLALYNGVALVLANLNSYFWNTRWTFRHRAKRRDARQRILFTVQALFNICVSNGLF
                     YALVHPVLIYTDVPAYLVGNVAKLISVVVASTLSFFVLRYLVFSRKRRFGGRL"
     gene            2150..2222
                     /locus_tag="VFT03_02985"
     tRNA            2150..2222
                     /locus_tag="VFT03_02985"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2183..2185,aa:Gly,seq:gcc)
     gene            2240..2310
                     /locus_tag="VFT03_02990"
     tRNA            2240..2310
                     /locus_tag="VFT03_02990"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2272..2274,aa:Cys,seq:gca)
     gene            2329..2400
                     /locus_tag="VFT03_02995"
     tRNA            2329..2400
                     /locus_tag="VFT03_02995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2361..2363,aa:Val,seq:gac)
     gene            complement(2411..>2852)
                     /locus_tag="VFT03_03000"
     CDS             complement(2411..>2852)
                     /locus_tag="VFT03_03000"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEU4847140.1"
                     /translation="MIVRGPGVPAGATRQQLVLNQDLAPTFADIAGASVPNFVDGRSF
                     LAVLGDSPPDAWRTGFLVRAQATDNRQLLMPPMPTNIALRTARYEYIDYPRGKDELYD
                     MDRDPYQIDNIQSSAPTDVLAQIRAQLKSLEGCGGDECREAEGP"
BASE COUNT          505 a          922 c          883 g          542 t
ORIGIN      
        1 ggccaacatc atgaagtaca ccgacggcct gtttcgcgac gtgttcttcg aggtcgccaa
       61 ggagtacgag aacgacttcg aggagataga cgaccgcatc gtggacaaca tgtgcatgca
      121 gctcgtcatg aagccgaacc tctacgacgt gctggtctgc cccaacctct acggtgacat
      181 cctctccgac ctgtgcgccg ggctcacggg aggccttggc gtcgcgccgg gggcgaacat
      241 aggagaggag atcgccgtct tcgagccggt gcacggctcg gctcccaagt acgccggcca
      301 gaaccgcgcc aacccgctgg ctaccctgct ctcggccaag aacatgctct accacctcgg
      361 ctacaatgag gagggcgacc gcatgcagcg ggggatagag gcggcgctgg ctagccccga
      421 gacgcgcacg aaggacctcg gagggtctgc cggcacgaag gagttcacgc aggctatcgt
      481 cgcgaacctc tagacgagag ggcagctccc gggccggtgc gcatcgcttt cttcactgag
      541 accttcttgc cagcgacgga cggtgttgtg accaggttgc gttacaccat cgaagagctc
      601 gcgaagatgg gcgatgacat gctcgtcgtc gcacccaggt accctgacgg tggccctgat
      661 tcttacgctg gtgccccaat atacagggtg ccgggcgtgc ctttcccgcc ttatcccagg
      721 ataaaactct ccttcgcgca tcctggcgtg ggccgtgctc tctggcgttt cgggccggac
      781 ctgatccacg cggtcaaccc ctttattctg ggtcctggcg ggatctacta ctccagaagg
      841 ctggaggtgc ccctggtagc ctcgtaccac accaacgtcg cagcctacgc ccgctactac
      901 aacctaaact tctcgtacaa cgccgcgcgc tggtggacca ggcagctcca caaccgggcg
      961 gagatcaacc tctgcacttc tgcgacgacc atggactacc tcctcaagga gggcgtgaag
     1021 cggatacgcc tgtggcctca aggggtggac gccaggcgct tccacccgga aaaggcctcc
     1081 caggtatggc gcgaaaggct ctccgacggg cacgcttcgg acagactttt gctctacgtc
     1141 ggccgcctcg cgcccgagaa gggcatcgag cggctcaagg cgatcctgac cggcgtgccg
     1201 ggaaccaggc tcgcgatcgt tggcgacggt ccggcaagag ctgatctcga acgcgagttc
     1261 agcggcaccg ccgcggtctt tactggggtc ttgcagggcg aagatctggc ctccgtctac
     1321 gcttcagcgg acgccttcct cttcccctct acgaccgaca ctctaggcct tgcgatgctt
     1381 gaggccctcg cctccggctt gccggttata gcagcgcgcg gcggcgcctc ccacgagatc
     1441 gtcagcgacg aagagaacgg gctactctac gaggccacct caacgccgtc gctcgtctcc
     1501 gccgtgcgcc gcctgttctc caacgaaggc ttccgcgccg cgctggcgcg ccgggcgcgc
     1561 gaaacggcag agaagcgcga ctgggaatcc tcgacccaca ccttgcacgg gtactacgag
     1621 gaggctatgg ggcggcgatg agcaccaagg gcacaagact caagcgtggc ggcgtccgct
     1681 tctcgaaatt taccgtcgtc gggctctcga acgccgcggt agacatcggg acgctcaacc
     1741 tgtttctgtg gctcgcgcct acgcgggacc cctctgtgct cgcgctctac aatggggtgg
     1801 ccctcgtgct cgcgaacctc aacagctatt tctggaacac gcgctggacc ttccgccaca
     1861 gggcgaagcg gcgcgacgcg aggcagagga tcctcttcac cgtgcaggca ctcttcaaca
     1921 tctgcgtgag caacgggctg ttctacgcgc tggtccatcc tgtcctcatc tacaccgacg
     1981 ttccggctta tctcgttggg aacgtggcca agctcatctc cgtggtggta gcctcgacgc
     2041 tgagcttttt cgtcctcagg tacctggtct tctcccgcaa acgacgcttc ggcgggcgct
     2101 tgtaagagcc aggtcagcgt gctaaattaa tccggcacgt gagagcgacg cggacgtagc
     2161 tcagctggta gagcatcacc ttgccatggt gagggtcgcg ggttcgaatc ccgtcgtccg
     2221 ctctggccgc agggcgactg gcggcatggc cgagtggtta ggcaagggtc tgcaaaaccc
     2281 tgtaccccgg ttcgattccg ggtgccgcct ctcggcgcgg gacgcagagg gcgattagct
     2341 cagggggaga gcgcttcctt gacgcggaag aggtcagtgg ttcgaatcca ctatcgccca
     2401 ctccagttgt ctacggacct tccgcttccc tacactcgtc gccgccgcag ccttccaacg
     2461 acttgagttg tgccctgatt tgagccagca cgtccgttgg tgcgctcgac tggatgttat
     2521 ctatctggta gggatctctg tccatgtcgt aaagctcgtc tttgccacgc gggtaatcga
     2581 tgtattcgta gcgcgcagtc ctgagcgcga tgtttgtggg catgggtggc atcaacagtt
     2641 gtcggttgtc tgtggcctgg gctcggacta gaaagccggt gcgccaggcg tccggcgggc
     2701 tgtcgcccag caccgccaag aaagagcgcc cgtcgacgaa gttcggtacc gaggcgcccg
     2761 ctatgtcggc gaaggtgggc gcgagatcct ggttaaggac cagctgctgg cgcgtcgcgc
     2821 ccgcgggaac ccctggccca cgcacgatca tc
//
LOCUS       DASTUV010000145         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_485672, whole genome shotgun sequence.
ACCESSION   DASTUV010000145 DASTUV010000000
VERSION     DASTUV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485672"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..883)
                     /gene="tpiA"
                     /locus_tag="VFT03_03005"
     CDS             complement(104..883)
                     /gene="tpiA"
                     /locus_tag="VFT03_03005"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU4847141.1"
                     /translation="MARRPMMAANWKMNKTVREAEEYVAALLPRAADADGVDVAVFVP
                     FTCLHEASRMAEHTPVIAGAQNFYYEDWGAFTGEVSVPMLQDAGAEAGIVGHSERREH
                     FCEDDDMVARKAARAVEAGLLPVVCVGETKEERDAGDMWEKVSGQVRRVMEELDDPDG
                     GSLVFAYEPIWAIGTGETATPEDAQDAIGRIRDLLRELRDDGFADGVRILYGGSVKPE
                     NASEIMAQEDVDGALVGGASLEVESFMQLVEAAQETVPSEQ"
     gene            complement(886..2073)
                     /locus_tag="VFT03_03010"
     CDS             complement(886..2073)
                     /locus_tag="VFT03_03010"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU4847142.1"
                     /translation="MNKQSVRDLDVNGKRALVRVDFNVPIKDGRVTDDTRIRRALPTI
                     RYLLEEGARPILISHLGRPKGEPDPQYAMDPVAKRLEELLGEPVLKLDAAVGPEVEKA
                     LDEWDGRGVVLLENSRFYPGETKNDPEFSEQLASLADLYVDDAFGAAHRAHATTVGVA
                     ERLPAAAGLLLEEEVDYLDGLLKDPERPFVAILGGAKVSDKLGVIESLLGVADTLLVG
                     GAMCFTFFKAQGYEVGKSLVEDDYQKEAKRLMDEAGEKLVLPVDVVVADEMEEGAEAE
                     VVRVDGIPPDKMGLDIGPRTVELFEEHILAARTIFWNGPMGVFEIDAFARGTEGVARA
                     VAKASERGATSVIGGGDSVAAVRKLGLEDEMSHISTGGGASLEYVEGKELPGVAALPE
                     KGA"
BASE COUNT          363 a          840 c          675 g          433 t
ORIGIN      
        1 ccacccttgc cggagtgcaa caggatgagt cctatgagcg cgatgcagaa cacggtgtga
       61 aagaaagcca gtacgtatag catgcgccta ctttatcaca cgatcactgc tcggagggta
      121 cggtctcctg ggctgcttcg acgagctgca tgaagctctc gacctcgagg ctcgccccgc
      181 cgaccagggc gccgtcaacg tcttcctggg ccatgatctc cgatgcgttc tccggcttga
      241 cggagccgcc gtagaggata cgaaccccat cggcgaaccc gtcgtccctg agctccctga
      301 gcagatcccg aatgcggccg atcgcgtcct gcgcgtcctc gggcgtggcc gtctccccag
      361 tcccgatcgc ccagatgggc tcgtaggcga agacgagtga accgccatcc gggtcgtcga
      421 gctcctccat cacgcgccgc acctgccccg agaccttctc ccacatgtcg ccggcgtcgc
      481 gttcctcctt ggtctcgccg acgcagacca cgggcaggag ccctgcctcg accgcgcggg
      541 cggctttcct ggcgaccata tcgtcgtcct cgcagaaatg ctcgcggcgc tcggagtggc
      601 cgacgatacc ggcctcagcc ccagcgtcct ggagcatcgg cacggagacc tcgcccgtga
      661 acgcgcccca gtcttcgtag tagaagttct gcgcgcccgc gatgaccggc gtatgctcgg
      721 ccatccggga agcttcgtgc aggcacgtga aagggacgaa gacggccacg tccacgccat
      781 cggcgtccgc cgcccgcggc aagagcgccg ccacgtactc ctccgcctcg cggacggtct
      841 tgttcatctt ccagttcgcc gccatcatgg gcctgcgcgc cattctcaag ctcccttctc
      901 cggcagggcc gccacgccgg gcagctcctt cccctccacg tactcgaggc tcgccccgcc
      961 tccggtggag atgtggctca tctcatcctc gaggccgagc ttccttacag ccgcgaccga
     1021 gtcaccgccc ccgatgacgc tcgtcgcgcc cctctcgctc gcctttgcga ccgctcgcgc
     1081 gacgccctcc gtgcccctgg cgaaggcgtc tatctcgaat acccccatcg ggccgttcca
     1141 gaatatggtt ctcgcggcca gaatatgctc ctcgaagagc tccacggtgc gcggcccgat
     1201 atccaacccc atcttgtccg gtggtatccc gtccaccctg acgacctcgg cctccgctcc
     1261 ttcttccatc tcgtccgcga cgaccacgtc taccgggagc acgagcttct cccccgcctc
     1321 gtccatgagg cgcttcgcct ccttctggta gtcgtcctcg acgagggact tgcccacctc
     1381 gtaaccctgc gccttgaaga acgtgaagca catggcgccg ccgacgagca gggtgtcagc
     1441 cacaccgagc aggctctcta tgacaccgag cttgtccgag accttggctc cgccgaggat
     1501 agcgacgaaa ggccgctcgg ggtctttgag aagcccgtcg aggtagtcca cctcctcctc
     1561 gagcaagagt cccgcggcgg ccgggagcct ctcggcgacc ccgacggtgg tggcgtgtgc
     1621 gcggtgggcc gccccgaacg cgtcgtccac gtagaggtcg gcaagagagg ccagttgctc
     1681 ggagaactcg gggtcgttct tcgtctcgcc gggatagaag cgcgagttct cgagcaacac
     1741 gacgcctctg ccatcccact cgtcgagcgc cttctcgacc tcgggtccga cggcggcatc
     1801 gagcttgagg acgggctcgc caagtaactc ttctagcctc tttgccaccg ggtccatcgc
     1861 atactgcggg tcgggctcgc ccttgggtct cccgaggtgg gagatcagga tcgggcgcgc
     1921 cccctcctcg aggagatacc ggatcgttgg cagtgctcgc cggatgcgcg tgtcgtccgt
     1981 cacgcgccca tccttgatgg gcacgttgaa gtccacgcgc acgagagctc gttttccgtt
     2041 tacgtcgaga tccctgacgc tctgtttgtt aattgcgcct cccttcggtc gtcgcagcac
     2101 gcttcggctt cgcctcagct tgtcagcacg ctccggcttc gcaggctctc agcacgtccc
     2161 ctaaagggga ctttcagcac gccgcctccc tccggtcggc ggcttgtcag cttctctcag
     2221 caccaagaga aagctgaaat gctgacaagc gagggcccct gggcccgagc gtgctagtag
     2281 cgtgcgaatg cccgcagtac cttcggcgtg a
//
LOCUS       DASTUV010000146         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1423977, whole genome shotgun sequence.
ACCESSION   DASTUV010000146 DASTUV010000000
VERSION     DASTUV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423977"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFT03_03015"
     CDS             complement(<1..986)
                     /locus_tag="VFT03_03015"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU4847143.1"
                     /translation="MLEGKKVVLAYSGGLDTSVCLKWLEQRGATPYALYLDLGQGEPA
                     DDVKNKALAIGAADAFVRDAKTEFAEEYVAPAIKANALYGGKYPLFTALGRPLIAKKL
                     VEAAREVGATHIAHGSTGKGNDQVRFDVTTASIAPDLTVVAPVRDWNMSRPEEMDYAE
                     EHGIPVPTTKESPYSVDANLWGRSIEAGPLEDPEHEPTDDVFELTASPEKAPDEPRYV
                     EVGFEEGLPTSLDGAELPMVDLIAELNRIAGEHGVGRVDMIEDRLVGIKSREIYEAPA
                     ALAIVQAHRELETLTLTKDVLRFKAAVEQRYAELTYDGLWFTPLKRALDAFI"
     gene            complement(1006..1227)
                     /locus_tag="VFT03_03020"
     CDS             complement(1006..1227)
                     /locus_tag="VFT03_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847144.1"
                     /translation="MGDPAESASLRLWVLEHRLAVCRGPLEFGLTGVLASLEVPLAES
                     KVGILAIARSQLDLAAQTLRERGHEVHEP"
     gene            complement(1232..1711)
                     /locus_tag="VFT03_03025"
     CDS             complement(1232..1711)
                     /locus_tag="VFT03_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU4847145.1"
                     /translation="MGATRAPADKATRQDLILRLISERNLGTQQGVVEALAKEGVEAA
                     QATVSRDLAELGVLKVGNRYLALPHDPGSAGIEVLPSFILSLVPAQNMVVIHTRDGTA
                     GAVSSVLDRVKGLKIVGTIAGQDTVLAVAPDNAAAEEVAGLIADVCRARTRPAGNVE"
     gene            complement(1726..>2547)
                     /gene="argC"
                     /locus_tag="VFT03_03030"
     CDS             complement(1726..>2547)
                     /gene="argC"
                     /locus_tag="VFT03_03030"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU4847146.1"
                     /translation="AAFVAYGHGESAAAVAGLLEAGARLVVDLSADFRLPDVEVYEEW
                     YGEHPAPGLLEEAHYGLPEVFGAPEGRLVANPGCYPTAAILALAPVVRRLGVRSVTVN
                     ALSGVSGAGAKPSVKTHFVTVNESVSPYGIVSGSPRHRHTPEIEMMLRRVGETPEVTF
                     VPHLLPISRGELETIVVELEEPKEMPGAGDVLGWYAEDYEGWGFVEARAEPPHVSHVA
                     NTNRARLSAAVDGRAGKLLLFAAVDNLLKGAAGAAVQNMNLALGYPEDLGLEHLK"
BASE COUNT          372 a          883 c          833 g          459 t
ORIGIN      
        1 tcgatgaagg cgtcgagggc tctcttgagg ggagtgaacc agaggccgtc ataggtgagc
       61 tcggcgtagc gttgctcgac ggcggccttg aagcggagga cgtccttggt gagggtgagg
      121 gtctcgagct ctctgtgggc ctggacgata gcgagggcgg ctggggcctc gtagatctca
      181 cggctcttga tgccgacgag gcggtcttcg atcatgtcca ccctgcctac gccgtgctcg
      241 ccggcgatcc ggttgagctc ggcgatgaga tctaccattg gcagctccgc gccgtcgagg
      301 ctcgtgggca ggccttcctc gaagccgacc tcgacgtagc ggggctcgtc gggggctttc
      361 tcgggagagg cggtgagctc gaagacgtcg tctgtaggct cgtgctcggg gtcttcgagg
      421 ggaccggcct ctatggagcg tccccagagg ttagcgtcca cgctgtaggg ggactccttg
      481 gtggtcggta cggggatccc atgttcctcc gcgtagtcca tctcctcggg gcggctcatg
      541 ttccagtcac gcacgggcgc gacgaccgtg aggtcggggg cgatggaggc tgtggtgacg
      601 tcgaagcgga cctggtcgtt gcctttgccc gtcgagccgt gagcgatgtg cgtcgccccc
      661 acctcgcgcg ccgcctcgac gagttttttc gctatgagcg ggcgcccgag cgccgtgaag
      721 agcgggtact tgccgccgta gagggcgttg gcctttatgg ccggcgcgac gtactcctcg
      781 gcgaactcgg tcttcgcgtc ccggacgaag gcgtcggccg cgcctatggc gagggccttg
      841 ttcttcacgt cgtccgcggg ctctccctgg ccgaggtcga ggtagagcgc gtacggcgtc
      901 gccccccgtt gctcgagcca cttcaggcag accgaggtgt ccagcccccc ggaataggcg
      961 agcactacct tctttccctc gagcatcttg cctccttgcg cgttatcagg gttcatggac
     1021 ctcgtggccc cgctcgcgca acgtctgcgc tgcgaggtcc agctgcgatc ttgcgatggc
     1081 caggatgccg accttgctct cggcgagcgg cacttccagt gacgcgagca cgccggtcag
     1141 cccgaactcc agcgggcctc ggcagacggc cagacgatgt tcgagcaccc agaggcgcag
     1201 cgaagcgctc tcggcaggat cacccacaaa cctactcgac gttgcccgct ggccgggtgc
     1261 gggcacggca gacgtcggcg atgagccctg cgacctcctc ggctgcagcg ttgtccggag
     1321 caacggcgag caccgtatcc tggcccgcta tggtcccgac gattttgagc cccttcaccc
     1381 tgtccagaac gctcgagacg gccccggcgg tcccgtcgcg cgtgtgtatg accaccatgt
     1441 tctgggccgg gacaaggctg aggatgaagc tcgggagcac ctcgatgccg gcggagccgg
     1501 ggtcgtgcgg gagcgcgagg tagcggttgc cgaccttgag gacgcctagc tcggccaggt
     1561 ccctgctcac cgtggcctgc gcggcttcca cgccctcctt ggcgagagcc tcgactactc
     1621 cctgctgggt gccgagattc ctctcggaga tgagcctgag tatgaggtcc tgcctcgtcg
     1681 ccttgtccgc cggcgcgcgt gtcgctccca aagtcctgat ccctcctact tgaggtgctc
     1741 gagcccgagg tcctcggggt acccgagcgc caggttcatg ttctgtaccg cagccccagc
     1801 cgcgcctttg agcaaattgt ccaccgccgc gaagagcaga agcttccccg cccgcccgtc
     1861 cacagccgcc gagagcctgg ctctgttggt gttcgcgacg tgcgagacat gcggcggctc
     1921 ggccctcgcc tccacgaacc cccagccctc gtaatcctcc gcgtaccagc cgagaacgtc
     1981 gcccgcgccg ggcatctcct taggttcctc gagctccacg acaatggtct cgagctcccc
     2041 gcggctgatg ggcaggaggt gcggcacgaa ggtcacctcg ggcgtctccc ccaccctgcg
     2101 cagcatcatc tctatctcgg gcgtgtgcct gtggcgtggc gagccgctca cgatcccata
     2161 cggcgagacg ctctcgttga cggtgacgaa gtgggtcttg acgctcggct tcgccccggc
     2221 cccgctaaca ccagatagcg cgttgaccgt cacggaacga acccccagcc gccgcaccac
     2281 cggcgcgagc gctagtatcg ccgccgtcgg ataacacccg gggttcgcta ccagcctccc
     2341 ctccggcgcc ccgaagacct cgggcagccc gtagtgcgct tcctcgagca atcccggcgc
     2401 gggatgctcc ccgtaccact cctcgtagac ctcgacgtcc gggagccgga aatccgccga
     2461 gaggtccacg accagcctcg ctccagcctc gagtaagccc gccaccgccg cggcgctctc
     2521 accgtgccca taagccacga aggccgc
//
LOCUS       DASTUV010000147         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_427749, whole genome shotgun sequence.
ACCESSION   DASTUV010000147 DASTUV010000000
VERSION     DASTUV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427749"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFT03_03035"
     CDS             complement(<1..684)
                     /locus_tag="VFT03_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU4847147.1"
                     /translation="MRLEIPVGERAIVLETGKLARQAGGSVTARLGDTMVLSTATRSE
                     APRPGATFLPLSVDIEERMSSAGKIPGGFIKREGRPSERAILTSRLTDRPLRPLFPKG
                     YHYEIQVIGTVLVADQETPYDTVSMVGASAALSLSDIPYDGPIGAVRVGRDPDGGFIL
                     NPTYQQLAESDLDLIVAGTKEAITMVEAGANEVTEDVMVEALGVAQDVIRQQAEAIEA
                     FAAEHGKEKQ"
     gene            complement(738..1004)
                     /gene="rpsO"
                     /locus_tag="VFT03_03040"
     CDS             complement(738..1004)
                     /gene="rpsO"
                     /locus_tag="VFT03_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU4847148.1"
                     /translation="MAVVQDKQEIIGEHRTHEGDTGSPEVQVAVLTRRIAHLTDHLRE
                     HKHDYHSRRGLLKMVGKRRRLLKYLQKKDVERYRSLIAKLGLRR"
     gene            complement(1078..1971)
                     /locus_tag="VFT03_03045"
     CDS             complement(1078..1971)
                     /locus_tag="VFT03_03045"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU4847149.1"
                     /translation="MNSVVALGNFDGVHLGHRAVVRRAVEEGRRRGAKVVAATFDPHP
                     RVVLAPGSEPRLLTTLEMRREELLGYGVDEVWAIRFDETLSRKSPEEFVRDVLVGEIG
                     ASAVVVGENFRFGHRAAGDFRELERLMRGFGGEAYAVRVRSEDGEAPISSTRIRRLVG
                     EGEVAEAAKLLGRPYVLRGEVVMGDKRGRTIGFPTANVLADPALVVPARGVYAGFVRV
                     GKDTYAACTNIGVAPTFERRESRVEAYLLGFEGDLYGREVDVSFLQRIREEKRFSGVE
                     ELKTQILRDVEAARRITSDAI"
     gene            complement(2006..>2781)
                     /gene="truB"
                     /locus_tag="VFT03_03050"
     CDS             complement(2006..>2781)
                     /gene="truB"
                     /locus_tag="VFT03_03050"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU4847150.1"
                     /translation="RVGHTGTLDPLASGLLVILIGRSTRLSRYVTDLDKAYTATARFG
                     AVSDTLDADGNITSLDAPVPDEAAIRATLPSFTGELLQVPPMASALKREGVRLYDLYR
                     RGVTIEREPRPVTVHAFDLIATDPAQQTATFEISCSSGTYVRTLIADLAESLHTGAYL
                     TALRRTRVGHLLVESALDPGDLSSDTIDNRIIHPSEAISHLPRVEVDGEEELAVRSGR
                     TLGARGLEGSFRVERGGVLLAVYRVDGAEARAEVVLCAA"
BASE COUNT          467 a          964 c          813 g          537 t
ORIGIN      
        1 ctgcttctcc ttgccgtgct cggcagcgaa ggcctctatg gcctcggcct gctggcggat
       61 gacatcctgg gccacgccga gcgcttcgac catcacgtcc tcggtcacct cgttggcgcc
      121 ggcctcgacc atcgtgatgg cctccttggt gccggcgacg atgaggtcta ggtcgctctc
      181 ggcgagctgc tggtaagtgg ggttgagaat aaacccaccg tccgggtcgc gcccgacgcg
      241 cacggccccg atcggaccgt cgtaaggtat gtcggatagc gagagcgcag ccgacgcgcc
      301 gaccatgctg acggtgtcgt agggcgtctc ctggtcggcg acgagcaccg ttcctatgac
      361 ctgtatctcg tagtggtagc ccttggggaa caggggcctc aagggcctgt ccgttagccg
      421 ggaggtcagg atagccctct cggaaggcct gccttcccgc ttgatgaagc cgccgggaat
      481 cttgcccgcc gaggacatcc tctcctcgat gtcgacgctc aggggcaaaa acgtggcgcc
      541 cggtcggggc gcctcggagc gggtcgcggt ggacagcacc atggtatcgc ccagcctggc
      601 cgtgaccgag ccaccggcct ggcgggcgag cttcccggtc tcgagcacga tcgcgcgctc
      661 gcctacgggg atctctaaac gcatattctt ttcttctcct ctttattctg cgatctaccg
      721 atactgcagg ccggaagcta gcggcgcaga ccgagcttgg cgatgagcga cctgtagcgc
      781 tcgacgtcct tcttctgcaa gtacttgagc agccggcgtc gcttgccaac catcttgagc
      841 aggcccctcc gcgaatggta gtcgtgcttg tgctcgcgca ggtggtccgt aagatgcgct
      901 atacgacgcg tgagcacggc gacctgcacc tcaggcgacc ctgtgtcgcc ctcgtgcgtc
      961 ctgtgctctc ctattatttc ttgtttgtct tgaactaccg ccaacttctt ctttctcctc
     1021 ttgttctctc gatatatctc agcataagat tctcaacatc atatgctatc acacgcatca
     1081 gatagcatcg cttgttatcc gtctcgccgc ctctacatcg cgcaagatct gcgtttttag
     1141 ctcctccaca ccggagaacc tcttctcctc ccttatcctc tgcaggaagc tcacgtccac
     1201 ctcacgcccg taaaggtccc cctcgaaccc gagcagatac gcctcgaccc ggctctctcg
     1261 ccgctcgaag gtcggagcca cgccgatgtt ggtgcaggcg gcgtaggtat ccttcccaac
     1321 ccgcacaaac cccgcataca cgccgcgcgc agggactacc agcgccggat cggctaacac
     1381 gttggctgtg gggaaaccga tggtcctccc cctcttgtca cccatgacga cctcgcccct
     1441 caagacgtag ggtctaccca gtagcttcgc cgcctcggcc acctcgccct caccgacgag
     1501 cctccggatg cgcgtcgagc tgatcggagc ctccccgtcc tcgctcctga cccgcaccgc
     1561 gtacgcctcc ccaccaaagc ctcgcatgag ccgttccaac tcccggaagt cgccggccgc
     1621 cctgtgcccg aaccggaagt tctccccaac gactacggcc gacgccccga tctcacctac
     1681 gagaacgtcg cgcacgaact cctcgggact cttcctcgat agcgtctcgt cgaaccttat
     1741 ggcccatacc tcatcaaccc cataacccaa caactcctcg cgccgcatct cgagcgtcgt
     1801 caagagcctt ggctcgctac ccggtgccag cactacccgt ggatgcgggt cgaaagtcgc
     1861 agccacgacc ttcgcccccc tcctcctccc ctcctctaca gcccgccgca ccaccgcccg
     1921 atgcccgagg tgtaccccgt cgaagttgcc caacgcgaca acggaattca tcgaattcat
     1981 cgcgtctcca caacgcgcac cgtctctagg ccgcgcacaa aactacctcg gccctcgcct
     2041 ccgcgccatc taccctgtac accgccagca ataccccacc ccgctccacc cggaagctac
     2101 cctcaagccc ccgcgccccg agcgtccgcc cactcctgac ggccagctcc tcctcgccat
     2161 ccacctcaac ccgcgggagg tgagagatcg cctcactcgg gtgtattatg cggttatcga
     2221 tggtgtcgga tgagaggtcg ccgggatcta gcgctgactc gacgagcagg tgtccgacgc
     2281 gggtccggcg gagggctgta aggtaggcgc cggtatggag gctctctgcg aggtcggcga
     2341 tgagggttct tacgtaggta ccgctggagc aggagatctc gaaggtagcc gtctgttgtg
     2401 ccgggtctgt ggctatgagg tcgaaggcgt ggaccgttac ggggcggggt tcgcgctcga
     2461 tggtgacgcc gcggcggtac aggtcgtaga gacggacgcc ctcgcgcttg agggcggagg
     2521 ccatcggggg gacctggagg agctcgccgg tgaagctggg gagcgtggcg cggatggcgg
     2581 cttcgtcggg cacaggggca tccagagagg ttatattgcc gtctgcgtcc agcgtgtcgg
     2641 agacggcgcc gaagcgggct gtagcggtat aggccttgtc caggtcggtg acgtaacgcg
     2701 agagtcgggt ggagcgtccg atcaggatca ccagcagccc ggaggccaga gggtcgagtg
     2761 tgccggtgtg tccgaccctg g
//
LOCUS       DASTUV010000148         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1700353, whole genome shotgun sequence.
ACCESSION   DASTUV010000148 DASTUV010000000
VERSION     DASTUV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700353"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFT03_03055"
     CDS             <1..196
                     /locus_tag="VFT03_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEU4847151.1"
                     /translation="ERPQGEFTRQLLLGENLDPDNVEANYENGVLTLAIPVAEKAKPR
                     RISVGSSGGQATIEGKSRRT"
     gene            complement(2200..>2784)
                     /locus_tag="VFT03_03060"
     CDS             complement(2200..>2784)
                     /locus_tag="VFT03_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847152.1"
                     /translation="PALMAEEKDHHFYPIEEPDVARAVRLWAGRADRVRLLTCKSPLC
                     AAQTDVLRRDFAKIGTRLSVRAVTDPYAVSAGNADVRMENWFLDEYDPSNLLGPPDSP
                     EAVLFATDPPLNGFGNHSERLESQAEAAQRLSGDRRRDAYAMLAQDILRTWAPWAVFE
                     QLGQPAFFSARLGCISSSPAYFGIDIARLCIRGD"
BASE COUNT          511 a          852 c          948 g          473 t
ORIGIN      
        1 agagcgtccg cagggtgagt tcacccgcca gctcctactc ggcgagaacc tggatcccga
       61 caacgtcgaa gcgaactacg aaaacggcgt gctgaccctg gcgatcccgg tcgctgagaa
      121 ggcaaagccg aggcgaatct ccgtgggcag ctcaggcgga caggcgacaa tcgagggcaa
      181 gtcccggcgc acgtagtcat cccgtccatg ggaggcgagg atcggttccg tggacccaac
      241 ggctctcgac tcactctgca gttctgaggg aggtgaatct ccgaatcgtc gagctcgctc
      301 cggatgcgga gacggtccag ctcctgtgcg agtgtggaga caacaactgc gtcgagcgag
      361 tgacgatcac ccgccggcgc ttcgacgagg tcatcgagcg tgacggtcgc gtattagcac
      421 ccgggcatcg atgagattct ggcagggtgc ctcgtccctg cggcgagttt gccgattacg
      481 ggcgcgttag aaactagggg cctaaccgcg aagggccgcg ttggtgacgc ggccctttcc
      541 ttgcgggaga cgagggctgg acgggtcctc aaaggggcct tgggaggagt cgccaaaacc
      601 aggcggctct catggcgatg ctacgccacc cgggtcgagg tctggcgaca agatcggata
      661 ccacgagcgt taccccattc tcgaggagtc gcccgatagc ggtctgggga cgctctcggg
      721 aaccgaggag aggggtccgc tatggaagga cacacatgct cgtccgactc gtgctcgtgc
      781 gcggaagtcg gcgagaagcc gggtaaggat acggcgagga tgaatccgag gcctgcactg
      841 cgactagtag ctgaaccgcc acccgactct ggcaagtagc gcggggccga cccgcgtttc
      901 gacgtcgcgg tccgcggata gaagattgcc tctcattccg ggcggctcga cgctgttggc
      961 acgagtcaag ccgtctctcg aggcccgtct cgctgtcaga ccggccccct cgattcgttt
     1021 tagaggcggg agtccgcgag ctggcccgaa ggtgccggag gtgcccggta cgacctacgt
     1081 cttctaagcc aacgaggagg tcgaggccga ccaccccgct gtgctcgatt gcccaggcgc
     1141 gtttgtcgcc tttgacgaga actgacgttg atggctcgcc tcaagggttg atgccgtggc
     1201 tgctcaccta atccttcact cgccgcctaa ttgcatcgat gcccttttgc atcttctcgt
     1261 acgcgggtgg gttggcggag tggacggcga tagaggaggg tggccgaaca ccccggagcg
     1321 cgacagcctc ttcgagccac aagacgaccg cgttgcccct ctccgtcgaa gatgcccagg
     1381 tcatggtcga gggagaggcg ctcgacgcaa cctccttcga gcagcgcgat cgcttcagca
     1441 ggcgtcttca cccacgtcca gccctcatcc ggtggtgggc ggggtcgtca agccaaaccg
     1501 acagagggac gcgttcagag ggcacgttga gactgtaacc gtgtgcctcc gggcctaatt
     1561 acgtcagcga tgaagaaggc atcaacgacc gctaggggct acggccaccg ccaccagcag
     1621 gagcgcaaac gctggtcgcc gcacgtcgag gcagggcagg tgttctgcgc ccgttgccgg
     1681 cagctaatcc ttccgggcga gccctggacc tcgggcacgt ggacgggtcg aagacgaagt
     1741 gggcgggccc cgatcatcga aggtgcaacc gccagacgga atggcacggc atcaagcgat
     1801 cgcggaggct cgggcggaca tcgcgtgagt ggtgagggag ggcgacggtc tcacataatg
     1861 cgaaccgggc ggcgaccgtc gcgggcccgt atcagcgtca cggtgcgccg acggaaggca
     1921 ctcaccctag cccgccgctc ggaatggcct ctaatcgtga tgcctgcaga cgaagtgtca
     1981 acagagcaat tccgggcatt tatcgaaact tgacccgctg ccctggtggt gtctcggtgg
     2041 tgtctcgctg ggcggcgtag cgttacgagc cgcatggttc ctaccgtgta ggactagtgc
     2101 gtgtagtcct ccactacccg gagcgtagcg gaaccccagg aaacatcggg ctcgttcttg
     2161 ccgccgaggc gtcggcgcag atcctcgctt gagcgacttc taatcgcctc tgatgcagag
     2221 ccgcgcgatg tcgatgccga agtacgctgg tgacgaggaa atgcagccga gacgtgccga
     2281 gaagaaggca ggctgaccca actgctcgaa gacggcccag ggggcccagg tgcgcaggat
     2341 gtcctgcgcg agcatcgcgt aggcgtctcg gcgccggtct ccgctcagtc tttgagccgc
     2401 ctccgcctgg ctttccaacc gctcggaatg gttgccgaac ccgttgagcg gcggatccgt
     2461 tgcgaacagg acggcctcgg gggaatccgg cgggccgagg aggttcgagg gatcgtattc
     2521 gtcgaggaac cagttctcca tgcgcacgtc cgcattgccg gccgagacgg cgtaggggtc
     2581 ggtgacggcc cggacgctta ggcgcgtgcc aatcttggcg aaatcacgtc gcaggacgtc
     2641 cgtctgcgct gcgcaaagcg gtgacttgca cgtcagcagg cgcacccggt cggcgcgccc
     2701 cgcccagagc cggacggcgc gcgcgacgtc gggctcctca atggggtaga agtggtgatc
     2761 cttctcctcg gccatgaggg ccgg
//
LOCUS       DASTUV010000149         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_950303, whole genome shotgun sequence.
ACCESSION   DASTUV010000149 DASTUV010000000
VERSION     DASTUV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950303"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1449
                     /locus_tag="VFT03_03065"
     CDS             223..1449
                     /locus_tag="VFT03_03065"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU4847153.1"
                     /translation="MKLSPAAVRALLLAVQGLDAPPERRAAKDDVLESIRKMGVLQID
                     SIAVVARSPYLVLWSRLGSYEPCWLDELLAEGSLFEYWAHAACFIPIEDYGLYRRFML
                     EGGEKSRAWFSAHPEEIGRVLARLREGGEVRSAEFARADGRAGGWWEWKPEKRALEHL
                     FAAGELMISRRDPNFHRVYDLREHVLKRALPGWEDALAPTSEEVRRALALKAVRSLGA
                     AVARWVPDYFRTPRKGIASLLDELADEGSLLRATIEDLDEPAYVHPDNAELARAAASG
                     GLRPSLTTLLSPFDPVVWDRARVLELFGFEYRIEVYTPAARRRYGYYSLPILHRGALV
                     GRLDAKAHRKQELFEVRALHLEPGVQATEDLLAGLAAALRGCADWHGTPEIVVRRSDP
                     AGLVEPLHAAIETDTL"
     gene            complement(1446..2189)
                     /locus_tag="VFT03_03070"
     CDS             complement(1446..2189)
                     /locus_tag="VFT03_03070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4847154.1"
                     /translation="MPEYDLLADLYDLEYSHDYDLPFWLSLADRQGDPIVEWGAGTGR
                     LAVPLVEAGFDVTALELSGWMVERGRKKSEVVEWVQGDMRRAELGRRYALAVCAFNSF
                     LCLLSVDDALAFLRNAREHLEPGGMLGIEVSAFYPEELVNPPGGPGLQHDFTRERPDG
                     RLDRFSVSRYDAASQLLAMRLYYELYGASGALEERRAHNLTIRVTWRDELGLVLRLAG
                     FEVEAVYGGFEGEAFTAESDHLIVLARRG"
     gene            2266..2826
                     /locus_tag="VFT03_03075"
     CDS             2266..2826
                     /locus_tag="VFT03_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU4847155.1"
                     /translation="MSTILLIVLVLVVVVLVIFAIATYNGLVRRRNETEEAYRQIDVQ
                     LKRRYDLIPNLLEGVKKYFRQEQDVLTQITQARSQAMQPQSVGEQAQSEARLSGAIGN
                     LFAVAENYPELRSNTVLLDFQEELSSTENKISFARQHYNDSVSDYNTKIQSFPAVIFA
                     RMMGFTEREYFEAVGPERESVTVSYD"
     gene            2829..3788
                     /locus_tag="VFT03_03080"
     CDS             2829..3788
                     /locus_tag="VFT03_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4847156.1"
                     /translation="MSESPYEGTFRERIAVNRRNSLILIVAFLAFVAVFGYVIGWAWI
                     GDPVGAIFGLVLAFIVGIVTGLATYYGGDRMVLAASRAREISHDDAPVLFNVVEEMAI
                     AAGLPMPKVYIIDDSAPNAFATGRDPEHASVAVTSGLLEKLNRDELQGVIAHEMSHVA
                     NFDIRYAMLVGILVGTTVLISDFFLRGLWFGGGGRGRGDGGGYIQLIMIVIAIVFAIL
                     APLFARLLQLSISRQREYLADASAVRLTRNPKGLADALQKISGDREVLEAANRATAHL
                     YIVNPVKGFEKRAKGLFSTHPPIEERIQILRFMETGGVAQPAG"
     gene            3838..3908
                     /locus_tag="VFT03_03085"
     tRNA            3838..3908
                     /locus_tag="VFT03_03085"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3870..3872,aa:Gln,seq:ctg)
     gene            4137..4322
                     /locus_tag="VFT03_03090"
     CDS             4137..4322
                     /locus_tag="VFT03_03090"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU4847157.1"
                     /translation="MPSRSSSREDKNEGKMDRAKGRAKEAGGALTGDRSKKSEGRSDQ
                     RKGKAKEKKGAAKDLLS"
     gene            4360..4518
                     /locus_tag="VFT03_03095"
     CDS             4360..4518
                     /locus_tag="VFT03_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847158.1"
                     /translation="MNGGKGIYVGGTLVSILISLALYATGRRESAIFVGLWAPTILNL
                     GQSLLDED"
     gene            4767..5015
                     /locus_tag="VFT03_03100"
     CDS             4767..5015
                     /locus_tag="VFT03_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847159.1"
                     /translation="MDRGPNKPKPKRGSAFEKLQREREAKGVEEDLERDVEEREERKK
                     IRQEEREERKQIRQEEREERKREREREREKARGQEESS"
BASE COUNT          983 a         1572 c         1653 g          828 t
ORIGIN      
        1 agacaacagg catcaggggt tgtggagagg ctctagttcg ccttggaaaa cccccagatc
       61 gcgacgcatg cgctatcgag ttccattacc tgaagcctga aacctgttcg tagtcgttag
      121 aacgaagcat ctgccgtata actagacggt tgttgacggt cggcgggagg tgggtcaggt
      181 gcggaccagc gcgggcgccg gagtactttc gaggaagggt cgatgaagtt atcaccggcg
      241 gcggtgcggg cgctcctgct ggcggtgcag ggtctggacg ctccgccgga gcgccgcgcg
      301 gcgaaggacg acgtgctgga gtccatacgg aagatgggtg tgctccagat cgactccata
      361 gccgtcgtcg cgcgcagccc ttacctggtt ctctggagca ggctcgggtc gtacgagcct
      421 tgctggcttg acgagcttct tgcggagggc tctctcttcg agtactgggc gcacgcggcc
      481 tgcttcatcc cgatcgaaga ctacggactg taccggcggt tcatgctcga gggtggtgag
      541 aagagcaggg cctggttttc ggctcacccg gaggagatcg ggcgggtgct cgcgcgcctg
      601 cgcgaggggg gagaggtgcg ctccgccgag ttcgcgcgcg ccgacgggcg ggccggcggc
      661 tggtgggagt ggaagccgga gaaacgggcg ctcgagcacc tgttcgccgc aggagagttg
      721 atgatctcgc gccgcgaccc gaacttccac cgtgtctacg acctgcgcga gcacgtgctg
      781 aaacgcgctc taccaggctg ggaggacgcc ctcgcgccga cttccgagga ggtgcggcgg
      841 gcgctcgcgc tcaaggcggt ccgctcactc ggcgcggcgg tggcccgctg ggtgcccgac
      901 tacttccgca cgcccaggaa aggcatcgca agtctgctcg atgagctcgc cgacgaaggc
      961 tccctccttc gagccacgat agaggacctg gatgagcccg cttatgtgca cccggacaac
     1021 gcggagctgg ccagagcggc cgcttccggc gggttgcggc catctctgac gaccctgctc
     1081 tcaccgttcg atcccgtggt ctgggaccgg gcgcgggtac tggagctctt cggcttcgag
     1141 tacaggatcg aggtctacac tccggcggcc cgccgccggt acgggtacta ctcgctgccg
     1201 atactgcacc gcggcgcgct ggtaggcagg ctcgacgcga aggcgcaccg caagcaggag
     1261 ctcttcgagg tcagagctct gcacctcgag cccggtgtgc aggcgaccga ggatctcctc
     1321 gccgggctcg ccgccgctct tcgcgggtgc gcggactggc acggcacgcc ggagatcgtg
     1381 gtgcggaggt cggatccggc agggctcgtc gagccgctcc acgccgcgat cgaaacagac
     1441 acactctagc ctctacgtgc cagcacgatg aggtggtcgc tctcagcagt aaacgcctcc
     1501 ccctcgaaac cgccgtagac ggcctcgacc tcgaaccccg ccagccggag cacaagccct
     1561 aactcgtcgc gccacgtcac tctgatcgtc aggttgtgcg cccgcctctc ctccagcgcg
     1621 cccgacgcgc cgtacagctc gtagtacagc cgcatcgcta gaagctgcga cgctgcgtcg
     1681 tagcgggaga cggagaagcg gtcgagccgc ccgtccggcc gctcgcgggt aaagtcgtgc
     1741 tgcagccccg gcccgccggg agggttcacg agctcttcgg ggtagaaagc ggagacctcg
     1801 atcccgagca tcccgccggg ctccaggtgc tcccgcgcgt tgcgtagaaa agccagtgcg
     1861 tcgtccacgc tcaacaggca caggaacgag ttgaaggcgc agacagcgag cgcgtaccgt
     1921 cttcccagct ccgccctccg catgtcaccc tggacccact cgacaacctc gctcttcttg
     1981 cgtccccgct ctaccatcca tccggagagc tccagggccg tgacgtcgaa gccggcctca
     2041 acgagtggga ccgccagccg ccccgtgccg gcgccccact cgacgatggg atccccctgc
     2101 cggtccgcca gagagagcca gaacgggagg tcgtaatcat gggagtattc gaggtcgtag
     2161 aggtcggcga ggaggtcgta ttcgggcaag gcggcgtcct ttatccggcg gcttgtggtg
     2221 aggatataat aatgctcgat gaatccttaa ccttggagga tctggatgag cacaattcta
     2281 ttgatagtcc tggtcctggt agtcgtagtg ctcgtgatct ttgccatcgc cacgtacaac
     2341 gggttggtgc ggcggcgcaa cgagaccgag gaggcttacc ggcagataga cgtgcagctc
     2401 aagcgccgct acgacctaat ccccaacctc ctcgagggcg tgaagaagta cttccgccag
     2461 gagcaggacg tcctcaccca gataactcag gcccgctccc aggcgatgca gccgcagagc
     2521 gtcggcgagc aggcccagtc cgaagcccgg ctctccggcg ccataggcaa cctgttcgcg
     2581 gtggcggaga actacccgga gctcaggtcc aacacggtgc tgttggactt ccaggaggag
     2641 ctctcttcga cggagaacaa gatctccttc gcgaggcagc actacaacga ctcggtcagc
     2701 gactacaaca cgaagataca gagcttcccc gcggtaatat tcgcccgcat gatgggcttc
     2761 accgagcggg agtacttcga ggccgtcggc cccgagcggg agtccgtgac ggtctcctac
     2821 gactagcgat gagcgagagc ccctacgagg gtacattccg cgagcgcata gcggtaaaca
     2881 ggcgcaacag tctgatcctg atcgtggcct tcctcgcgtt cgtagccgtc ttcgggtacg
     2941 tgatcgggtg ggcctggatc ggagaccccg taggcgccat cttcggcctc gtcctcgcct
     3001 tcatcgtcgg catagtcaca ggtctcgcca cctactacgg cggcgacagg atggtgctag
     3061 ccgcctcgcg ggcgcgggag atctcccacg acgacgcccc cgtcctcttc aacgtcgtcg
     3121 aggagatggc catagccgcc ggtctcccca tgccgaaggt ctacatcatc gacgactcgg
     3181 cccccaacgc cttcgcgaca ggccgggacc ctgagcacgc ctccgtcgcc gtgacctcgg
     3241 ggcttctgga gaagctgaac agggacgagc tgcagggcgt catagcgcac gagatgtccc
     3301 acgtcgccaa cttcgacatc cgctacgcta tgctagtagg catcctggta gggaccacgg
     3361 ttctgatctc cgacttcttc ctgcgcggcc tctggttcgg cggaggcggt agagggcgcg
     3421 gggatggcgg tgggtacata caactgatca tgatagtcat cgccatagtc ttcgccatcc
     3481 tggccccgct cttcgcgcgt ctgctccagc tctccatctc ccgccagcgc gagtacctcg
     3541 ccgacgccag cgccgtcagg ctcacccgca accccaaggg attggctgac gctctgcaga
     3601 agataagtgg cgacagagag gttctcgagg ccgccaaccg cgccacagcc cacctctaca
     3661 tcgtcaaccc ggtaaagggc ttcgagaagc gcgcgaaggg tctcttctcc acccatccgc
     3721 cgatagagga gaggatacag atcctgcgtt tcatggagac cggcggcgtc gcgcagcccg
     3781 ccggatagaa gctcgacgga aagccactcg cgcgttacaa tacgccccgt cgccttttgc
     3841 cggatggtgt aacggcagca cgactgactc tggatcagtt agtgaaggtt cgaatccttc
     3901 tccggcagct actaaaatcc ggcacatcgc aaggtatagt cgcgtgcgcc gacctcgtgg
     3961 cactgaggga tcgctacgca gcgtagaacg cccacccaga ccgggttacg gcgcagatca
     4021 ccccctccta gcaacgggcc cgattcttct ctcacttgtg cgtggcgtat cgttggcagg
     4081 cctccgcgag tctaagacta caagtagaca agaacggtga ggagaggagc gcaaccgtgc
     4141 ccagcaggag cagcagtagg gaggacaaga acgagggaaa gatggacagg gccaagggcc
     4201 gggccaaaga ggccggaggt gcgttgacag gcgacaggag caagaagtcc gagggacgct
     4261 ccgaccagag gaagggcaaa gccaaggaga agaagggcgc cgccaaagac cttctgagct
     4321 agcgctagcc cataactaac ccacaaggag gtagagatcg tgaacggtgg caaaggaatc
     4381 tacgtcggag ggacactcgt ctctatcctg atctcgctcg cgctgtacgc gacgggcagg
     4441 cgcgagtcgg cgatcttcgt tgggctgtgg gccccgacca tcctcaacct gggtcagagc
     4501 ctcctagacg aagactaggg tcaagaaagg gctggagctt ctacgagctt cggcccttct
     4561 tgtcctcaat tcaccgaagc tcgtagaagg caggagttct ccgaagtctg catccagcat
     4621 agccccggtc ctatgctcgc tgcacggacg tgctgtacgg tgcggcggct ctttgccgat
     4681 cggaccggtg gaagaaggat tacaccaccc ccccgctggg aacaattacg gcgcgcccgc
     4741 gcaataccga ccagaaggag gatggcgtgg accggggacc gaacaagccg aagcccaagc
     4801 gggggtcggc tttcgagaag ctgcagcgcg agcgggaggc gaagggtgta gaggaggacc
     4861 tggagcggga cgtggaggag cgagaggagc gcaagaagat caggcaggag gagcgcgagg
     4921 agcgcaagca gatcaggcaa gaggagcgag aggaacgcaa gcgggagcgg gagcgggagc
     4981 gtgagaaagc gcgaggtcag gaggaaagct cctagtgtga gcgaaacggg ccggag
//
LOCUS       DASTUV010000150         5736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1521601, whole genome shotgun sequence.
ACCESSION   DASTUV010000150 DASTUV010000000
VERSION     DASTUV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521601"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1092)
                     /locus_tag="VFT03_03105"
     CDS             complement(112..1092)
                     /locus_tag="VFT03_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4847160.1"
                     /translation="MKAILIERFGDPSELRQKEVPRPEPTEGEVLIEVHAAAVNRSDV
                     LNARGSFPFTTLPRIPGRDFAGVVVEGPQELVGTDVWGTGGGELGFVRDGTHAQYLAV
                     SRNAVAPKSETLSLAEAAASGLAYVTAGSALVDLGRVLAGETILVTGAAGGVGSAAVM
                     IARWKSARVIGAIKDESERAMAERAGVEVIVDTSREEMTDAVLAATNGEGADLVLDAV
                     GGPLFEPALNSLAKKGRMVVITTTPDKQHVSFNLFDFYRKGLRLFGLMTSFLDAEGSA
                     AVLRDLSPGFDEGALRAPAIADRYPLEQAGMAYARVESGEAAGRVLLLMS"
     gene            complement(1251..2672)
                     /locus_tag="VFT03_03110"
     CDS             complement(1251..2672)
                     /locus_tag="VFT03_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4847161.1"
                     /translation="MPPARTLLRGGHVISMDPDIGDIPGGDVLIEGEDIAAVAPSIDA
                     SDCEVVDASGAIVIPGFIDSHRHTWETVVRGIAPDVTLDGYFALILDTLAPVYRPQDV
                     YAGNLLGTLEAIDAGVTTLLDWSHINNTPDHADEAIRALSELGIRAVYCYGNPNTSLA
                     DWWFNSILESPEDIRRIRDRYFSSDEGLMTLAMGTRGPGFCVPEVVKHDWDLARDIGV
                     PISVHVGMGNVAGRFRMVEQLDDLGLLGPDTTYIHCNHLTEREFQLIADTGGTTSIAP
                     MVEMTMGHGMPPTGELLDHGLRPSLSCDVVTTVPGDPFTQMRFLFAAERVRVHERVFD
                     QELEEVPPLLSSRDVLEFATVEGARVCGLADRTGSLTPGKKADIVMLDIERVNAAPVI
                     DPVGTIVCSMDTSNVNSVWVNGRALKRDGQLVGFDLERARRLAEDSRDFLVTHTERPA
                     HWATPRTTSGATPGVADSSSLDT"
     gene            complement(2769..3608)
                     /locus_tag="VFT03_03115"
     CDS             complement(2769..3608)
                     /locus_tag="VFT03_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEU4847162.1"
                     /translation="MSVESARVSNDRLQKVLPDAIAAIHGVIEKHRVTEEEWSAVLAF
                     LTEVGRQDEFILLSDVTKTSVLVDAISHEGETGVTPSDVEGPLYREDPPWREKPVKIY
                     EEYEGAENGDVLFVHGRVTSADGTPVSGAVLDIWQTGPDGGYDLWDERQPDYNFRGRF
                     GVDGDGSYEFQTMVPKPYTVPTDGPVGRLLNATGQHPWRPAHIHVKVEAQGHEPLITQ
                     VFFPEDPYLENDTIGAVKPALVRPLTRHENEEELARRSFDAPFYTCEFDITLKPAISA
                     ARA"
     gene            complement(3772..4653)
                     /locus_tag="VFT03_03120"
     CDS             complement(3772..4653)
                     /locus_tag="VFT03_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU4847163.1"
                     /translation="MKLVTYLDGQLRVGAVVGSGVVDLSERFDSVPALIEEGEPGLEE
                     ARELAEKTDPLAEVGEVRLRAPIPHPRRNVFCMGWNYLTHFEEGRGRRGDQKEELPEY
                     PTFFDKPTTTVIGPYDDIPFDPNFSEKIDYEAELAIVMGRAGRSIPAAEAMDHIFGYT
                     VANDVTARDVQRRHGGQWLKGKGMDGSCPLGPWIVTADEIVDPQNLQVQCRVNGVEKQ
                     NSNTRFMVFPIAKLVEELSLAMTLLPGDILLTGTPEGVGYARTPPEYLSPGDEVTVTV
                     TGIGEITNRVVERSLTR"
     gene            4960..>5736
                     /locus_tag="VFT03_03125"
     CDS             4960..>5736
                     /locus_tag="VFT03_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4847164.1"
                     /translation="MSDLPEETPSVWGPDSRRAGVSVTFDNLGEAADLERGQWPENEP
                     LGRHFSVKRTLPRILDTLEELALRATFFVEGINAELNSEALLEISNSGHELGYHGWRH
                     EFWPNLNPSNEARLLERGVRKMDELGVRPRGFRPPGGRLTRSSPELLESLGFTLCSPA
                     GTGIGFLGNLVVLPFEWRLIDAYYYLPRFGGLRETATGSSEPLPPVRFWDTLSSALQR
                     VVRDGRHLTLVFHPFLEEQEDRFGIMRGALEAVRDLAQDGL"
BASE COUNT          970 a         1900 c         1731 g         1135 t
ORIGIN      
        1 ccctcctttc ttctcaatcg ctggcgcacc gatgaacgaa ctcccatacc tcaatccagg
       61 aaccacttcg atcggccaga aggtgtattg gacgttccag cgatcggctg ctcaactcat
      121 gaggaggagt accctaccgg ccgcctcacc gctctctacc cgagcgtagg ccatccctgc
      181 ctgctctagc gggtaacggt cggcgatcgc cggcgcacgt agggcccctt cgtcgaaccc
      241 cgggcttagg tctctcagga cggcggcgct cccttcggcg tccaaaaacg acgtcatcag
      301 cccgaagagg cgcagcccct tgcggtaaaa gtcgaacagg ttgaaggaga cgtgttgctt
      361 gtcgggagtc gtcgtgatga cgaccatgcg tcccttcttg gcgaggctgt tcagggccgg
      421 ttcgaacagg gggccgccga cagcgtcgag gacgagatcc gctccttccc cgtttgtggc
      481 cgccaggaca gcgtctgtca tttcctcccg agaggtatcg acgatgacct cgacgcccgc
      541 ccgctctgcc atcgcacgct ccgactcatc cttgatggcc ccgatgacgc gggcgctctt
      601 ccagcgggcg atcatcaccg ccgcgctccc cacgccgccg gcggcgccgg taacgagaat
      661 agtctccccg gccaaaaccc tgcccaagtc gaccagcgca gacccggcgg ttacgtaggc
      721 gaggcccgat gcggctgcct ccgccagaga gagcgtttcg gatttcggcg ctacggcgtt
      781 cctggagacg gcgaggtatt gggcatgggt cccgtcgcgg acgaacccga gctccccacc
      841 gcccgtgccc cagacatctg tccccaccag ctcttgcggc ccttcgacga cgaccccggc
      901 gaagtcgcgt cccggtatgc gaggcagggt ggtgaaaggg aacgagcctc gtgcgttcag
      961 cacgtcgctg cggtttacgg cggcggcgtg gacctcgata agaacttcgc cttccgtcgg
     1021 ctctggtctc gggacctcct tctgccggag ctccgatgga tcaccgaagc gttcgatcag
     1081 gatggccttc attctgcgaa acacctccac gagtctttcg agggatcggg gttcaagtga
     1141 acgcctccgc gaggtaagac tccgcagtaa caccgcccat ctagaactcg ctcttggtga
     1201 gagagcccga actcgaccac gccgagtcat ccatcgcagg ctcgttgcgc tcaggtatca
     1261 aggctcgatg agtcggcgac gccgggcgtc gccccgctcg tggtccgcgg ggtggcccag
     1321 tgcgcaggcc gctcggtgtg tgtcaccagg aagtcccgcg agtcctcggc caaccggcgc
     1381 gctcgctcga gatcgaagcc gacgagttgc ccgtctctct tgagggcgcg tccgttgacc
     1441 cacacggagt tcacgttcga ggtgtccatg ctgcagacga tcgtccccac cgggtcgatg
     1501 accggagccg cgttgacacg ctcgatgtcg agcatcacta tgtccgcctt cttgccgggc
     1561 gtgagcgagc cggtcctgtc ggcgagcccg cacacacgag ccccttcgac ggtggcgaac
     1621 tcgagaacgt cccgcgacga tagcagcggc gggacctctt ccagttcctg gtcgaagacg
     1681 cgctcgtgga cccgcacccg ctcggccgcg aacaggaacc tcatctgcgt gaacgggtcg
     1741 ccgggcacgg tggttacgac gtcgcagctg aggctgggac gcaagccgtg gtccagcagc
     1801 tctccggtcg gaggcatgcc gtgccccatc gtcatctcga ccatcggagc gatcgaggtg
     1861 gtgccgccgg tgtccgcgat gagctggaac tcccgctctg tcaggtggtt gcagtggatg
     1921 taggtggtgt ccggaccgag caaaccgagg tcatcgagct gctcgaccat cctgaagcgt
     1981 cctgcgacgt tgcccatccc gacgtgcacg ctgatgggca cgccgatgtc cctggcaaga
     2041 tcccagtcgt gcttgaccac ctcggggaca cagaaccctg gacccctggt gcccatggcg
     2101 agggtcatga gcccttcgtc ggacgagaag tacctgtccc ttattcggcg aatgtcttcg
     2161 ggcgactcga gaatgctgtt gaaccaccag tcggcaagcg aggtgttagg gttgccgtag
     2221 cagtacacgg cccgtatgcc gagctcggag agggcccgga tcgcttcgtc ggcgtggtcg
     2281 ggcgtgttgt tgatgtgcga ccagtcgagg agcgtcgtca cgcccgcgtc gatcgcctcg
     2341 agcgtgccga gaaggttgcc ggcgtacacg tcctgaggac ggtagaccgg cgccagggtg
     2401 tcgaggatca acgcgaagta cccgtcaagc gtaacgtccg gcgcgatgcc gcgcaccacg
     2461 gtctcccagg tgtgcctgtg cgagtcgata aaccccggga tgacaatcgc acccgaggcg
     2521 tcgacgacct cgcagtcgga cgcgtcgatg gacggagcga cagccgcgat atcctcaccc
     2581 tcgatgagca catccccgcc tgggatgtcc cctatatctg gatccataga gatgacgtgg
     2641 ccaccgcgga gcagcgttcg tgcaggaggc atctttgttc taacctttct tcctcgtaac
     2701 gaccgggcgt cccgttgcgc ctggcccgtt cgcgcaacaa gcgtgtcttt cgacctttgg
     2761 gaagcatctc aggcccgcgc agctgatatc gccggcttca gcgtaatgtc gaactcgcag
     2821 gtgtagaacg gagcgtcgaa gctgcgccgc gccagttctt cctcgttctc gtgcctggtc
     2881 agcggcctga cgagggccgg tttgaccgcc cctatggtat cgttctccag gtacggatct
     2941 tccgggaaga agacctgcgt gatgagcggc tcgtgtcctt gcgcctccac cttgacatgt
     3001 atgtgcgccg gacgccacgg gtgctggcct gtggcattta gcagcctgcc cactggtccg
     3061 tccgtcggga ccgtgtacgg tttgggcacc atcgtctgaa actcgtagct gccgtccccg
     3121 tcgacgccga acctccccct gaagttgtag tccggctgcc tctcgtccca gaggtcgtag
     3181 ccgccgtctg ggcccgtctg ccagatatcg aggaccgcgc cacttacggg cgtgccgtcc
     3241 gcggaggtga cccgaccgtg gacgaagagt acgtctccgt tctccgcgcc ttcgtactcc
     3301 tcgtagatct tcaccggctt ctcgcgccag ggcgggtcct cccgatagag tggtccttcc
     3361 acgtcgctcg gcgtaacgcc ggtctcgcct tcgtgggaga tggcatcaac cagtaccgag
     3421 gtcttcgtga cgtcggagag caggatgaat tcgtcctgcc ttccgacctc ggtgaggaaa
     3481 gcgagcaccg cggaccattc ctcttccgtc acccggtgct tctcgatcac cccgtgtatg
     3541 gctgcgatgg catcaggcaa gaccttctgc agcctatcat tactcaccct cgccgactcc
     3601 acgctcatct ctcctttccc gcattaccgc tacgcgccat tatggctttg cccacttgat
     3661 acgtccaata ctgggcacag agtcaggtct tggtagagtc gattccaccc ctaggcgtac
     3721 cgctgctact ttatccaacc ccttgcacaa cggctaactc tttaggttct cctagcgggt
     3781 tagggatcgt tcgacgaccc tgttcgtgat ctccccgata ccggtgacgg tgacggtgac
     3841 ctcgtcgccc ggactcaggt actcgggagg ggttcgggcg taaccgaccc cttcgggcgt
     3901 gcccgtcagg agtatgtccc cgggcaacag ggtcatcgcc agagagagct cctctacgag
     3961 cttggcgatg gggaatacca tgaatctggt gttggagttc tgcttctcca caccgtttac
     4021 acgacactgt acctgcaggt tctgcgggtc tactatctca tcggccgtga cgatccaggg
     4081 tcccagcggg cagcttccgt ccatcccctt gcctttgagc cactggccgc cgtggcgccg
     4141 ctggacgtcc ctggccgtaa cgtcgttggc gaccgtgtag ccgaagatgt ggtccatggc
     4201 ctctgcggcc ggtatgctcc tcccggcgcg gcccatcacg atcgcgagct cggcttcgta
     4261 gtcgatcttc tcggagaaat tcgggtcgaa ggggatgtcg tcgtagggac cgatcaccgt
     4321 cgtcgtcggt ttgtcgaaga aggtcgggta ctcgggcaac tcctctttct ggtctccgcg
     4381 gcgccctcgc ccttcctcga agtgcgtcag atagttccac cccatacaga agacgttcct
     4441 cctgggatgc gggatcggag cccgtaacct gacctcgccg acctctgcca gcgggtcggt
     4501 cttctcggcg agctcgcggg cctcctcgag gccaggttct ccttcttcga tgagcgccgg
     4561 cacggagtcg aacctctcgc tcaggtcgac cactccgctg ccgacgacgg ctcctacgcg
     4621 tagctgaccg tccaggtacg tgacgagttt catcgaatca cctccgcaca ttgacgcggg
     4681 tagaggtggc gagctcgacg accttcgtga ggtcaccttc gctctcccgg tcatcgtaga
     4741 tgatgaccat ctggcgccgg catcatgcaa tactccttcc accaattccc tacgaaccat
     4801 tctgacggca ggagacagat ccgtccaaca catctgcttg attgcttgat acataattag
     4861 tataaagcga tagtgaacct gcgacgcttg cgctactccg tggccatgac gggcacccct
     4921 gacaccgact cctgatcccc tgttcgagcc ggagaacaca tgagtgacct accagaggag
     4981 actccttccg tctggggccc cgactcccgc cgcgcggggg tcagcgtgac cttcgacaac
     5041 ctgggggagg cggccgacct cgagcgtggg cagtggcccg aaaacgagcc gctcgggcgc
     5101 cacttctcgg tgaagcgtac gctcccccgc atcctggata cgctcgagga gctagcactt
     5161 cgggcaacgt tcttcgtcga agggatcaac gccgaactca actccgaagc actcctcgag
     5221 atctccaact ccggccacga gcttggctac cacggctggc gccacgaatt ctggcccaat
     5281 ctgaacccct ccaacgaggc ccgtctgcta gaacggggcg tacgcaagat ggacgaactc
     5341 ggagtccgac cgcgcggctt tcggccgccg ggcgggaggc tgactcgttc ctcgccagaa
     5401 ctgctcgaga gcctcggttt tactctctgt tcgccggcgg gcaccgggat cggctttctc
     5461 ggcaacctgg tcgtcctgcc gttcgagtgg cgcctgatcg acgcctacta ttacctgccg
     5521 cgcttcggag gtcttcggga gaccgccact ggatcgagcg agccgctgcc cccggtccgc
     5581 ttctgggata cgctaagctc ggcgctgcag cgcgtcgtcc gcgacggcag gcacctaacc
     5641 ctggtgttcc atcccttcct ggaagaacag gaagatcgct ttgggattat gcgcggagcg
     5701 ctcgaagcgg tgcgggactt ggcacaagat gggttg
//
LOCUS       DASTUV010000151         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_672265, whole genome shotgun sequence.
ACCESSION   DASTUV010000151 DASTUV010000000
VERSION     DASTUV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672265"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            749..2146
                     /locus_tag="VFT03_03130"
     CDS             749..2146
                     /locus_tag="VFT03_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="HEU4847165.1"
                     /translation="MAISTVNPATGEEVRTFEALSEAEVDEKIQRAADTFREYRKTSL
                     AERAEMMQRAAKILDDEAAEFGRLMTSEMGKTLAAATAEAKKCARGCRYYAENAGRFL
                     ADEAAELDGRAFVRYQPIGPILAVMPWNFPFWQVFRFAAPALMAGNVGLLKHASNVPQ
                     CALAIEDIIHRAGFPEGAFQTLLINSDQVQSVIDDPRVRAVTLTGSEGAGRSVGGEAG
                     EEIKPSVLELGGSDPFIVMPSVDLEKAVSTAVTSRTLNNGQSCINAKRIIVHEEIADD
                     FERRFVERMSALKVGDPMQEDTDMGPLATPQILEDVDTQVRESVDAGARVLTGGKPLD
                     GPGNFYPPTVITDIPEESPAYREEIFGPVASLFRARDIDDAIRIANDTSFGLGSSAWT
                     NDPSEQERFVNEIEAGMVYVNRMTESTPEVPFGGVKNSGYGRELSHYGIQEFTNIKTV
                     WIADGAVSEGAAASE"
     gene            2223..2381
                     /locus_tag="VFT03_03135"
     CDS             2223..2381
                     /locus_tag="VFT03_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847166.1"
                     /translation="MRNRLLLLGSAWGVLLAALPTLVMARLYAVAGGTLLGPAFGRLV
                     HRAVRGEP"
BASE COUNT          473 a          752 c          790 g          437 t
ORIGIN      
        1 acggcctctc gtggcactac atcttgtgag cgaatacgct ccctcgtgag agcagctacg
       61 atgcatagtg cgctacgtag tggtggcggg ggtttagcgc gcatctctcc ccgccccagt
      121 gcttgaggga gtactggtta ctcagagaga gggggtgaag agatggctga ggcagcaaga
      181 cttagacgag gttgtctaag gcgcaggagg cgctgccgcc gcaggagcag caaccttcgt
      241 cgcaggcgtt gtcgatcgcg tttgcgccgc cgctgccgcc acagcaggcg tcggcactaa
      301 ccagcgtcaa gcgctagtta gttccgggaa ggtcccgaca cacatgttgc ggggccttct
      361 ttttttgtag cataggtttg agggtcgcca gagtaagcgt ggcaaaactc aggcgtgcgg
      421 ttggcttcct ttcgtcggta acacaggaag agggcgggtt cgtggccgct ggaggctttg
      481 atcaatcctt ccccttcttg cccttcttca cccaagtgtt ccatggcctg gcaaaggcca
      541 ccctgacgct atgaaaacga cctgtcccag gttcttttca caccagtgtc aaaagatgtt
      601 ctggcgtgct gcggcaactc atagtaattg aaatagccgc atagataagc ggttatccaa
      661 cagtgattag acaacttggt cagagggtac acgcaaagtg tatatttact tcaacggggt
      721 gaaggatcct ttatcaagga ggagcatcgt ggcgatatcc accgtcaacc cggctacggg
      781 cgaagaggtc aggacgttcg aggcgctgag cgaggcggag gtggacgaga agatccagcg
      841 cgccgccgac acgttccgcg agtacaggaa gacctccctg gccgagagag ccgagatgat
      901 gcagcgggcg gcaaagatcc tggacgacga ggccgcggag ttcggccgcc ttatgacgag
      961 cgagatgggc aagactctcg ctgcagctac cgccgaggcg aagaagtgcg cccgcggctg
     1021 ccgctactac gccgagaacg ccgggcgttt cctggcagac gaggcggctg agctcgacgg
     1081 cagggccttc gtccgctacc agcccatagg ccccatactc gcggtgatgc cgtggaactt
     1141 ccctttctgg caggtcttcc gcttcgctgc cccggcgcta atggccggca acgtgggtct
     1201 gctcaagcac gcttccaacg tgccgcagtg cgcgctggcc atagaggaca tcatccacag
     1261 ggccggcttc ccggagggcg cgttccagac gttgctgatc aactcggacc aggtgcagtc
     1321 cgttatagac gacccgcgcg tgcgggcggt gacgctcacg ggcagcgagg gcgcgggccg
     1381 gagcgtaggc ggcgaggcgg gggaggagat caagcctagc gtgctcgagc tcggcgggag
     1441 cgatccgttc atcgtcatgc cgagcgtgga cctcgagaag gccgtgagca ccgccgtcac
     1501 ctcgcgcacg ctcaacaacg ggcagtcgtg catcaacgcc aagaggataa tcgtgcacga
     1561 ggagatcgcc gacgacttcg agcgccgctt cgtcgagagg atgtcagcac tcaaggtggg
     1621 agacccgatg caggaggaca ccgacatggg tccgctcgcc acacctcaga tcctcgaaga
     1681 cgtggacact caggtcagag agagcgtcga cgcaggagcg cgcgtcctca ccggcggcaa
     1741 gcccttggac ggaccaggca acttctaccc gccgaccgtg atcacggaca tacctgaaga
     1801 gtcgccggcg tacagggagg agatcttcgg ccccgtcgcc tcgctctttc gcgcgaggga
     1861 catagacgac gcgatccgca tagccaacga tacgagcttc ggcctcggct ccagcgcctg
     1921 gaccaacgac ccctctgagc aggagcgctt cgtgaacgag atagaggccg gcatggtcta
     1981 cgtgaaccgg atgaccgagt ccaccccgga ggtccccttc ggcggcgtga agaactcggg
     2041 ctacggccgc gagctctccc actacggcat ccaggagttc accaacatca agaccgtctg
     2101 gatcgccgat ggagccgtaa gcgagggcgc cgccgcgtct gaataatcca ctatcgcgtg
     2161 aaagctgttg ctgacgggtc cgggaccctt gggctaacct cacgcatgag gtgatggtaa
     2221 ccgtgcgcaa cagactcctc ctcctcggct ccgcgtgggg cgtgctcctc gcggccttgc
     2281 cgacgctcgt gatggcgcgc ctctacgcgg tggccggcgg caccctgctc ggcccagctt
     2341 tcgggcggct cgtccaccgc gccgtgaggg gggagccgtg atcctcctcc gcctcctcgg
     2401 cgtcggcgcc gtctacggtc tcggcttcat gttcgtcaag tcgctcttcc cc
//
LOCUS       DASTUV010000152         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1271606, whole genome shotgun sequence.
ACCESSION   DASTUV010000152 DASTUV010000000
VERSION     DASTUV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271606"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..575
                     /locus_tag="VFT03_03140"
     CDS             192..575
                     /locus_tag="VFT03_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4847167.1"
                     /translation="MRYVHTCYRVLDLERSIDFYTNKLGLELARTVPIGDDATNAFLA
                     VPGDPEPRLELTLNHDREEPYSLGEGYSHVAFAVEDLDVLAQKLEAAGGVDFESKPHA
                     MSSGSRIFFVSDPDGYRIEFIERSR"
     gene            646..1266
                     /locus_tag="VFT03_03145"
     CDS             646..1266
                     /locus_tag="VFT03_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4847168.1"
                     /translation="MGAPLPKTRDFRRNERGFTLQELMTVVAILGILIAIAVITFLGL
                     LERWRVEAAADQFAADLRLAHARATNQLTDWRVVIMPDGSPVRGCSSADYCMIKLSAP
                     YEGGGSTPTMDSETPPGPRELPEGTKIKEVTFNPDCSGGDGGALIPPSSCGGTRTLEF
                     SSNGTVRTLRPGQSGTVRISSEDGSPWCDIVFTSATSHIRIGPLAY"
     gene            1256..1639
                     /locus_tag="VFT03_03150"
     CDS             1256..1639
                     /locus_tag="VFT03_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4847169.1"
                     /translation="MRTRRESGYSLVEVMAAIVILTTAIIPMVGMFDAALRAVSTSGD
                     YGVARACAGQKLEQLRSLPYETVSAGLPDGTCEPSGLSYAFHEEFVSAELQGIQEDQG
                     LTRVTVTVSWDGGDSYATTGVVSRW"
     gene            1636..2181
                     /locus_tag="VFT03_03155"
     CDS             1636..2181
                     /locus_tag="VFT03_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4847170.1"
                     /translation="MSRSSFPRDESGFTLPELLVTMTMMLTVLFALYSIFDTSVRIFR
                     HGKDELKAVEDARLGLERMEREIRAAYPRDDGTLISVAGEREITFQNRPDNDPPETIT
                     YSLSSGSSSYLRRNGQRVVGPLDGADGLVFAYCTSATDCSPTIAEERSIKLVRMTLNV
                     RIPGPTHATQTLTTDVFLRNG"
     gene            2185..2607
                     /locus_tag="VFT03_03160"
     CDS             2185..2607
                     /locus_tag="VFT03_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847171.1"
                     /translation="MRRVVEDESGMVMGLAVIMVALIGVIGAGLLTLVVTDLHATIEA
                     NRGQRAFEMADAGVEVAKARLAEAPDLTGWSSGVLRMAGGEEGEVTVAIERREGEGGP
                     YFVATSTGRWGSARRRIEATFSVMDGEPRLLSWRELYE"
     gene            complement(2629..2823)
                     /locus_tag="VFT03_03165"
     CDS             complement(2629..2823)
                     /locus_tag="VFT03_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847172.1"
                     /translation="MSETRLACRVCDGVFSEHQVRLKTVDPEEDQVQCPNCGSSRMEP
                     YAFDPDGPTDNPFEKEDEGG"
BASE COUNT          579 a          957 c          983 g          542 t
ORIGIN      
        1 cgaagccgaa accatagcgc gcccccacgc gggcgatcgt ggccgctctc ctggccggac
       61 tctccggtat ttggcgcgtg acgttatcca taggaaaagc atatcagcag cgacgcaata
      121 ggctacaggc atcagcaaaa acctgtaacc tgatgcgtga tacctgaaac ctacggacga
      181 aggagcgcaa cttgcggtac gttcacactt gctacagggt cctcgacctg gagaggagca
      241 tagatttcta cacgaacaag ctgggcctgg agctcgcccg tacggtcccc atcggcgacg
      301 atgcgaccaa cgccttcctg gctgtccctg gcgaccccga gcctcgcctc gagctgaccc
      361 tcaaccacga ccgcgaggag ccttactccc taggcgaagg ctacagtcac gtcgccttcg
      421 ccgtcgagga tctcgatgtc ctggcgcaaa agctcgaggc ggccggtgga gtagacttcg
      481 agagcaagcc ccacgccatg agcagtggca gcaggatctt cttcgtcagc gacccggacg
      541 gctaccgcat agagttcatc gagcgtagtc ggtagagcga ggggagccgc cgcctctcct
      601 cccgctacag cttcacagcc ggctcgccga taacccctct ggatcatggg cgcgccgctt
      661 ccgaaaactc gcgactttcg acgaaacgag cggggattca ccctgcaaga gctgatgacc
      721 gtcgtcgcca tcttggggat cctcatcgcg atagccgtga tcaccttcct ggggctcctg
      781 gagcgctggc gggtggaggc cgccgccgac cagttcgccg ccgacctgcg cctcgctcac
      841 gccagggcga ctaaccagct gacggactgg cgcgtcgtga tcatgccgga tggctcgccg
      901 gtcaggggtt gctcgtcggc tgactactgc atgatcaagc tgagcgctcc ctacgaaggg
      961 ggcggctcca cgcccacgat ggactccgag accccgcccg gcccgcgcga gcttcccgaa
     1021 ggcacgaaga taaaagaggt caccttcaac ccagactgct cgggcggcga cgggggggcg
     1081 ctcattcccc caagcagttg tggcggcacg cggacgctgg agttcagctc caacgggacg
     1141 gtccggaccc tgcgcccggg gcagagcggt acggtcagga tcagctccga agacggcagc
     1201 ccctggtgcg acatagtgtt cacctcggcc acgtcgcaca taaggatcgg tccccttgcg
     1261 tactaggagg gagtccggct actcgctggt agaggtgatg gcggccatcg tcatcctcac
     1321 caccgctatc atcccgatgg tcggaatgtt cgacgcggcg ctccgggcgg taagcacgag
     1381 cggggattac ggcgtcgcgc gggcgtgcgc cggtcagaag ctggagcagc tccggagcct
     1441 cccttacgag acggtgagcg ccggcctgcc cgacggcacc tgcgaacctt cgggtttaag
     1501 ttatgcgttc catgaggagt tcgtgagcgc tgagcttcaa ggaatccagg aggaccaagg
     1561 cctgaccagg gtgaccgtga cggtgagctg ggacggcggc gactcgtacg ccacgacggg
     1621 ggtcgtgtcc aggtggtgag ccgaagctcc ttcccgagag acgagagtgg attcacgctc
     1681 cccgagttgc tggtcaccat gaccatgatg ctcaccgtgc tattcgccct gtactcgatc
     1741 ttcgacacga gcgtccggat cttccgtcac ggcaaagacg agctcaaagc ggtcgaggac
     1801 gcccggctgg ggctcgaaag gatggaacgt gagataaggg cagcatatcc acgggacgac
     1861 ggcaccctca taagcgttgc tggagagcgc gagatcacct tccaaaacag gccggacaac
     1921 gatccgccgg agacgatcac ttacagcctg agcagcggct cgagcagcta ccttagaagg
     1981 aacggccaga gggtggtggg gcctctggac ggagccgacg gcctcgtgtt cgcctactgc
     2041 accagcgcaa cagactgctc gcccacgatc gccgaagaga ggagtatcaa gctggtgcgg
     2101 atgacgctca acgtgaggat acccggaccc actcacgcga cccagaccct cacgaccgac
     2161 gtattcttga ggaacggcta gcaggtgcga cgggtcgtcg aagacgagtc gggcatggtc
     2221 atgggcctcg cggtgatcat ggttgctctc atcggggtaa taggagcggg gttgctgacc
     2281 ctcgtcgtca ccgacctcca cgccacgata gaggccaacc ggggacaacg cgcgttcgag
     2341 atggccgatg ccggcgtcga ggtcgccaaa gcacggctcg ccgaagctcc tgacctgaca
     2401 ggctggtctt cgggggtgct ccgtatggcg ggtggagagg agggggaggt cacggttgcc
     2461 atcgagcggc gcgaaggtga gggtggtccg tacttcgtgg cgacatcgac cggccggtgg
     2521 ggaagcgcga ggcgcaggat cgaggccacc ttctccgtga tggacggcga gccgaggctc
     2581 ctgagctggc gagagctcta cgagtagggg gccttcgcga accgccccct acccgccctc
     2641 gtcttccttc tcgaacgggt tatccgttgg gccatcaggg tcgaaggcgt agggctccat
     2701 gcgggaggag ccgcagttcg ggcactggac ctgatcctct tcaggatcca ccgtcttcaa
     2761 gcgcacctgg tgttcggaga acaccccgtc gcatacgcgg cacgccagcc tggtctcgct
     2821 catgcgcgct ccttcctctc gcgttgctcg caatatatac cccggcgtgc gccctcgcac
     2881 ttcttaaccc gctcttaacc gttctctctg tcgcgggcct gctaccctgt gtagacttct
     2941 tatcactcgg cacgatacac ggtggggtgc gtatggtttc tggggacacg atggtagtat
     3001 ttgtctggag gtgccctgtc tgttcggggg tatacgaggg tgaagcattg ttcttcttgc
     3061 a
//
LOCUS       DASTUV010000153         3255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1342457, whole genome shotgun sequence.
ACCESSION   DASTUV010000153 DASTUV010000000
VERSION     DASTUV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342457"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFT03_03170"
     CDS             <1..853
                     /locus_tag="VFT03_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4847173.1"
                     /translation="TELSLMLAAVFWGTNYAATKYAAEFIPPLLIVGIRFTVGGLLML
                     GLLQILEPASKLATKDLLPVAGLGCLGVAIGQTAFTFGVSMTSAANTGLIFATAPVWG
                     LLLGSILGLERPTPRGILGVGLSILGVGIVFYEGLGVKGTSLLGDCLVLLAAMGFGAY
                     TVLSMPVLERRSPLAVATYSLLFGGLAVLLLSAVYLAGLEWGSVGMGAWAAVAFSAIF
                     ATAFAFSAWQTGISRKGANRVLVYQYLITVTGVASGIVFFGEVLGIEKLIGGAVILVG
                     VYLARRQ"
     gene            919..1971
                     /locus_tag="VFT03_03175"
     CDS             919..1971
                     /locus_tag="VFT03_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEU4847174.1"
                     /translation="MIPERVPERRYVIFAEGWFASRHAKTAHGLLRYGKDEVAAVVDS
                     TLAGRRVRDVTPELERDAPIVGSIEEALEYSPTSVLVGLAPAGGRLPEDWMASLERAA
                     DAGLEIVSGLHQRLAPQVPDKKVWDVREPPQDIPLFSGEGFGVEPKIALTVGTANAIG
                     KMTATLEVQRAAREAGLEAEFIATGQTGIIIAGWGISVDAVVSDFVAGASEQLVLEAA
                     RRGPDLILVEGQGSLGHPAYSGVTLGLLHGSCPDCLILCADASSEEVVTGVPRPSPAR
                     AARLYEEVAALVKPAPVVAVSLNTGDLDDDAEAEEFVARVSEETGLPAADPFRGSAAP
                     ILEAVLAAPKTSAAGF"
     gene            complement(2143..2526)
                     /locus_tag="VFT03_03180"
     CDS             complement(2143..2526)
                     /locus_tag="VFT03_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847175.1"
                     /translation="MSPATSVLAYALWPSLTLAIIILWLAAMLDITRHRRRGTYTSAG
                     LLGMAILVSVVAVIAVTGISAVLAAGWIDATSRASFAGIQTGKLEGSARAYIFSFGLE
                     NASYGSPADTPGKKDSQDREFRHAP"
     gene            complement(2553..>3255)
                     /locus_tag="VFT03_03185"
     CDS             complement(2553..>3255)
                     /locus_tag="VFT03_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU4847176.1"
                     /translation="YIRGAISSGRLRPGDRLPPEMDLAQHLGVSRPTVREALKVLEAL
                     NVLESSTGPTGGTFVRSLDGTGVAEYLNDSITLLLDIDELTLEELWTAREAIEIPAVG
                     MAAIRRTEQDLAAMRKNIDAYFPDITFHTTIANASKNRLLSLFMVSIGMTIRTQSERY
                     TMPEAKKISQEQHRLIYEAILDQDEVLAQTRMKEHLQVAYEVYRQAAPKKAVDRISEK
                     PSSDSLRDASLSGTS"
BASE COUNT          558 a         1022 c         1049 g          626 t
ORIGIN      
        1 gacggagcta tctttgatgc tggccgccgt cttctggggc accaactacg cagcgacgaa
       61 atacgcggcc gagttcatac cccctctgct aatagtgggg attcgcttta ccgtcggtgg
      121 actcttgatg ctcggcctcc tgcaaatcct ggagccagcc agcaagcttg ccacgaagga
      181 tcttcttccc gtggcgggac tggggtgcct cggggtagcg atcggccaga cagcctttac
      241 cttcggcgtg agcatgacca gcgccgccaa caccggcctg atcttcgcca cagccccagt
      301 gtgggggctg ctattaggct cgatactcgg gctggagaga cccacgccga gaggcatact
      361 gggagtgggg ctctcgatcc tgggcgtcgg catcgtgttt tatgagggcc tcggggtcaa
      421 gggcacgagc ctcctcggcg attgcctggt gctcctggcg gccatgggtt tcggggccta
      481 caccgtcctg tcgatgcccg tgctcgagcg ccgctcgccg ctcgcggtgg caacctactc
      541 tctgctcttc ggcggattgg ccgtcctgct gctctctgcc gtgtaccttg ctggtctgga
      601 gtggggtagc gtgggcatgg gagcctgggc ggcggtagcc ttctcggcca tcttcgctac
      661 ggccttcgcc ttctcggcct ggcaaacggg catctcgcgc aaaggagcca acagggtact
      721 ggtctaccag tacttgataa ccgttactgg ggtcgcatcg ggcatcgtct tctttggcga
      781 ggtactcggg atcgagaagc tgatcggcgg ggcggtcatt ctcgtgggcg tgtacctggc
      841 ccggcgccag tgatccagat gagcgtcgcg ataacgagtt ggttgccgtc ggtaccgccg
      901 gcttgttaca ttgaaggtat gatcccggag agagtacccg agcgccgcta cgtgatcttc
      961 gccgagggtt ggtttgcgag tcgccacgca aagaccgccc acggtttgct gcgctacggc
     1021 aaagacgagg tggcggccgt cgtcgactcg acgctcgcgg gccgcagagt gcgcgacgtc
     1081 acgcccgagc tcgaacgcga cgcgcccata gtcggcagta tcgaggaagc gctcgaatac
     1141 tccccgacct cggtcctcgt cgggctggcc ccggcgggcg ggcggctgcc cgaggattgg
     1201 atggcaagcc tcgagcgagc cgctgatgcg gggctcgaga tcgtcagcgg tctccaccag
     1261 cgcctcgcac cacaggtgcc agacaagaag gtctgggacg tgcgcgagcc gccacaggac
     1321 atcccgctgt tctcaggcga gggcttcggg gtggaaccga agatcgccct caccgtcggc
     1381 acggcaaacg ccataggcaa gatgaccgcc acgctggagg tgcagcgagc agcccgggaa
     1441 gccggccttg aggccgagtt catcgcgacg gggcagacgg gcatcatcat cgcgggctgg
     1501 ggtatctccg tcgatgcggt ggtctctgac ttcgtcgccg gagcctcgga gcagctggtg
     1561 ctggaggcgg ccagaagagg tccggatctg atcctggtcg aaggccaggg ctctctgggg
     1621 cacccggcct actcgggggt gacgctgggg ctcctgcacg gctcgtgccc tgactgcctc
     1681 atcctgtgcg ccgatgcctc cagtgaagag gtagtgacgg gcgtcccgcg cccgagccca
     1741 gcacgcgccg ccagactcta cgaggaggtc gccgcactgg tcaagccggc gcccgtggtg
     1801 gcggtgagcc tcaatacggg agatctcgac gacgacgccg aggccgaaga gttcgtcgca
     1861 cgagtgtccg aggagacggg cctgccggcc gccgacccgt tccggggctc cgccgccccg
     1921 atcctggaag ccgtgctcgc agcgcccaag actagcgctg caggcttctg aacccgcgtc
     1981 ccacctcgaa agacccgcac caattctcga atagttgatc ccggcaagaa gtcgggagag
     2041 cccgcccctc agaaaacaac aagctgacaa gtcgcctgag gcgaagccag ggcgacgtgc
     2101 tgacaagcga aggcggagcc tgagcgtgct gacaagcccc cgtcaggggg cgtgtcgaaa
     2161 ttcgcggtcc tggctatctt tcttgccagg tgtatcagcc ggcgatccat acgaggcatt
     2221 ctccaggcca aaactgaaga tgtaagcgcg tgccgaacct tccagcttcc cagtctggat
     2281 cccggcgaag cttgctctcg aggttgcgtc tatccacccg gcggcgagga ccgcgctgat
     2341 gccggtgacc gcgatgactg ctacgaccga aacgaggatt gccataccga gcagccccgc
     2401 gctcgtgtac gttcctctgc gcctgtggcg tgtgatgtcc agcattgccg caagccacag
     2461 gataattatc gccagggtta acgagggcca cagagcatac gcgaggacgg aagtcgcagg
     2521 agacatgtcg tgtcggatcc tcgacggtcc ccttaagacg tccccgatag agaggcgtcc
     2581 ctgaggctat ccgaagatgg tttctccgag atccggtcca ccgccttttt gggggccgct
     2641 tgccggtaga cctcgtaagc cacttgcaaa tgttccttca ttcgcgtctg ggccagcacc
     2701 tcatcctggt cgaggatggc ttcgtaaatc aaccggtgtt gctcttgaga gatcttcttc
     2761 gcttcgggca tggtgtagcg ctcggattgt gtcctgatcg tcatgcctat agagaccatg
     2821 aacagactta gcagtctgtt ttttgaagcg ttggctatgg tcgtatggaa cgttatgtcc
     2881 gggaagtagg cgtcaatgtt cttgcgcatc gccgcgagat cttgctccgt ccgccggata
     2941 gcggccattc ccaccgccgg tatctctatg gcctccctgg ccgtccacag ctcctccagc
     3001 gtcaactcgt ctatgtcgag cagcagggtt atggagtcgt tcaggtactc cgctaccccg
     3061 gtgccgtcga gcgatcttac gaacgtgccc cccgtgggac ccgtcgagct ctccagcacg
     3121 tttagcgcct cgaggacctt gagcgcctcc cgcaccgtcg gccggctgac cccgaggtgc
     3181 tgggcaagat ccatctccgg cggcagcctg tcgccggggc gtaacctccc cgaagagata
     3241 gcgccgcgta tgtaa
//
LOCUS       DASTUV010000154         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_498330, whole genome shotgun sequence.
ACCESSION   DASTUV010000154 DASTUV010000000
VERSION     DASTUV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498330"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFT03_03190"
     CDS             <1..354
                     /locus_tag="VFT03_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847177.1"
                     /translation="RLFWRNALSLATRYLKTAGVTEEKMSAMLRPISPLALKPLLGRE
                     RRAIFASVADRVVPATEAASLWRRWEEPRIVWYQGTHNAFLGTTEGRTLLATVLREAG
                     IVSPEVVTAPRKSGR"
     gene            441..1073
                     /locus_tag="VFT03_03195"
     CDS             441..1073
                     /locus_tag="VFT03_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU4847178.1"
                     /translation="MAASRSTPGGEYDLSNPVGGFVDVARRVLLQPASFFAAMPRSGP
                     LLIPLVFAIICIEISVLLSALLVLVGVQRNPGFNPNPQTVLPSVYTPGSALVSIILAP
                     IFGAIGLFIAAAIQQLLVRLVVGANNSGYPATFRVAAYTQVTGLINWIPILGPLLSLY
                     GLYLAIVGIREAHDTTTGKAVLVILIPFAVALLVALIVLLTVGIAFLSQI"
     gene            complement(1107..2213)
                     /locus_tag="VFT03_03200"
     CDS             complement(1107..2213)
                     /locus_tag="VFT03_03200"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU4847179.1"
                     /translation="MASESSRGSLSTPLCDLLGVRYPVIQAPMAGGWTTPELVSAVSN
                     AGGFGSLAGAGVPPERLREEIRAVKVRTDRPFAVNFLLAPPGPGSHDVAAVQRFLDGF
                     RERFDLPPGETELESPSFSLQEQLEVVFEERVPVLSVALGDPGELVVRAHEDDMLVVS
                     MVTTVADAVRAADGGVDVVVAQGAEAGGHRSVVELGSDGEPPLVGTLALAPQVVDAVG
                     VPVVAAGGIVDGRGLVAALALGAAGVQMGTRFLLARESGAHPAYRRRLISATETDTVV
                     TRVFTGRPARGLRNHFVEEYLSAGSEPLAWPLQRAAAGDIYRASQAADDGDYSPLLAG
                     QALRMLEEDGQGAAGIVEEIVAEAGTVLSRLAAP"
BASE COUNT          431 a          861 c          763 g          408 t
ORIGIN      
        1 cgtctgttct ggcgcaacgc cctctccctg gccacgcgtt atctcaagac cgctggcgtg
       61 accgaggaga agatgagtgc gatgctgcgc ccgatctccc ccctcgccct gaagcccctt
      121 ctcgggcgcg aacgccgggc catcttcgct agcgtcgccg accgcgtcgt gcccgcgacc
      181 gaggcggcca gcttgtggcg ccgttgggag gagccgcgca tagtctggta ccaaggtacc
      241 cataacgctt tcctcggcac caccgagggt agaacgctgc ttgccaccgt cctgcgagaa
      301 gccggcatcg tctcgcccga ggtcgtaacc gcgcctcgca aaagcggtag gtgagagacc
      361 gggctaccga ggcggtcagg tgttgcgccc gcgccgggtc gggtaatatg gaggggcgag
      421 cggatcaagg gggtaagggg atggctgcga gccggtctac accaggcgga gagtacgacc
      481 tatccaaccc ggtaggtggc tttgtggacg tggcgcgccg ggtgttgctt caaccggcga
      541 gcttcttcgc cgcgatgccc cggagcggac cactcctgat ccccctggtc ttcgctatca
      601 tctgcatcga gatatccgtg ctcttgagcg cgctgctcgt gctggtaggt gttcagagga
      661 acccaggttt caatccgaac ccccagaccg ttttgccctc ggtctacacc ccgggctcgg
      721 cgctggtgtc cataatattg gcgccgatat tcggtgcgat cgggctcttc atcgcggccg
      781 cgatccagca actactcgtc aggctcgtcg tcggggcgaa caactcgggc tatccagcca
      841 ccttcagggt ggctgcctac actcaggtca cagggctgat caactggatc cccatactcg
      901 gacccctgct ctcgctgtac ggtctctacc tcgccatcgt gggtatccgc gaggcgcacg
      961 acacgacgac gggcaaggcc gtgctggtca tcctgatccc gttcgccgta gccctactcg
     1021 tagccctgat cgtgctgctc acggtgggga tagcgttctt aagccagatc tagcgagcct
     1081 ggcacattga agagctcacg gggatctcaa ggtgcggcca gccgcgatag cacggtcccg
     1141 gcctctgcga cgatctcctc gacgatcccg gccgctccct gcccgtcttc ctcgagcatc
     1201 ctcagggcct gcccggcgag gagcggcgag taatcgccgt cgtcggcggc ctgggacgcc
     1261 ctgtagatgt cgccggcggc ggccctctgc aacggccagg ccaggggttc tgagccggcg
     1321 ctcaggtact cctcgacgaa gtggttgcgc aatcccctgg cgggccgccc ggtgaagacc
     1381 cgggtcacga cggtgtcggt ctccgtagcc gagatcagcc gcctccggta agcgggatgc
     1441 gcgccgctct cacgggcgag caggaaccgg gtgcccatct ggacacccgc cgcgccgagc
     1501 gccagggcag cgacgagccc gcggccgtcc acgatcccac ctgccgcgac gacagggacc
     1561 cccaccgcat ctacgacctg cggggcgagc gccagcgtcc cgacgagcgg cggctcgccg
     1621 tcggaaccaa gttcgacaac ggaacggtgc ccgcctgcct ccgcaccctg ggccaccacc
     1681 acgtcaaccc cgccatccgc ggcgcggacg gcgtcagcaa cagtagtgac catggacact
     1741 actagcatgt catcctcgtg tgcccgcacg acgagctcgc ccgggtcccc gagcgcgacg
     1801 ctcaacaccg gcacccgctc ctcgaagacc acctcgagct gctcctgcag cgagaatgat
     1861 ggtgactcga gctccgtctc tccgggaggg agatcgaatc gttctctaaa cccgtcgaga
     1921 aagcgctgga cggcggcgac gtcgtggctc cccggccccg gcggggcgag gaggaagttg
     1981 accgcaaagg gccggtccgt gcgcactttc accgcccgga tctcctcccg cagccgctcc
     2041 ggcggcacgc ccgcaccagc cagcgagccg aacccaccgg cattcgagac cgccgagacc
     2101 agctcggggg tcgtccaccc tcccgccatg ggggcctgga tcacagggta acggacgccg
     2161 agcagatcgc acaacggcgt cgatagtgac ccgcgcgatg attccgaagc cacaagacac
     2221 ctcccggtct ctaagttcct tgcatattcg tcagatgcac cagggtaccc ttatgcaact
     2281 ataccttcga gccaggaacg gcggagggca cgcaagtatc ctctttcaca aacaacccct
     2341 tcgaaaccta tcgatggagc aggcgcgcac cttcctggat caactcccgg tgagcaccgc
     2401 tgatagaggc cggataggac acggtaacgc ggagcgcctc ctgcgactat aagttagggt
     2461 acc
//
LOCUS       DASTUV010000155         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_556593, whole genome shotgun sequence.
ACCESSION   DASTUV010000155 DASTUV010000000
VERSION     DASTUV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556593"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..2953)
                     /locus_tag="VFT03_03205"
     CDS             complement(152..2953)
                     /locus_tag="VFT03_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU4847180.1"
                     /translation="MAAGSERLTFDEVGRLPASGDNVAIATRRIEAGTRVAHERSEFT
                     VPHTVLEGHRFAVERIGEGEDLLSWGLTFGQATKDIEPGDYACNEKILRVLRERHGTS
                     PSLDGDEDPEGTHDQGGGRLPGGYRRAGLDLPDQPNFRDAELEAYVLDEETFRPRAQV
                     PPYDEPRSFMGYRRGAGRGVGTRNYVVVMGVTSRLTGFVRALEWEMKGFADACENVSG
                     IVCVAHTEGGEDRTPNNLDLLLRTLSGFMVNPNVGAVLSLDHGGQEAVTNEMLRSYLE
                     DNSYPIEDVPHEFMSLEGNFRADLERAKSVVSGWLEEVNGARRTEEPVSELKIGLQCG
                     GSDAFSGVSANPLVGWVSKEIVRNGGAANLAETDELIGAERYVLRHVRDLDTARRFLR
                     TVERFKERVGWHGHTAEDNPSGGNNYRGLYNISIKSIGAARKKDPDVRIDHVIDYAER
                     MGEGGFYFMDSPGNDLESVAGQVAAGASMIFFTTGNGSITNFPFVPTIKVVTTTGRYD
                     LLSRDMDVNAGAYLDGTPMEELGRDMFERTLRAASGEKTVGERAGHSQVSIWRDWKQT
                     SAENLEELRNAPEPDGEPLPVKPGDDELEFSFQAIASERGPVADQIGLVMPTSLCSGQ
                     IARRIADRLNEREATRGKVTRFVALPHTEGCGVSAGSAERIYSRTVLGHLANPTVRFA
                     LLLEHGCEKTHNDYFENFLRERGLDPTRFGWASVQLDGGIDSVVEKVEDWFAETLEAA
                     GELTHEPAGLGALRLGLHASGPLSDAAALSLARATRAVVGAGGTVVVPETAAMLSSPV
                     YLERVLGDLVVKNTVAYGQAVREPGFHVMEAPTNHWVETATGLGATGVEIMAAHVAGR
                     PLQAHRMIPLLQASSDPGTLRSHGDDLDVLLEEDPSSWAEQMLSAVAAVASREYTPRL
                     FAAGNTDFQFTRGLLGVSM"
     gene            complement(3025..3264)
                     /locus_tag="VFT03_03210"
     CDS             complement(3025..3264)
                     /locus_tag="VFT03_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847181.1"
                     /translation="MDGIEPQVEGRLAVREQEAAFLVVRAEDPDDWLVRFEKSGDFPA
                     REWAENMVRVYNRRLSQKSAGPPTPPGTRPASYHP"
     gene            complement(3270..3650)
                     /locus_tag="VFT03_03215"
     CDS             complement(3270..3650)
                     /locus_tag="VFT03_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU4847182.1"
                     /translation="MTQGEKRREAHVVGDVSDFPEGSQKVVRIGRRQIGVFNIGGVLY
                     GLPNVCPHQTGPLCEGKPPLGTLVSRAENDWRFEWVHDGEVVVCPWHGLEYHVPTGRC
                     LAYPNINLRRYEVVVESGKVKVLV"
     gene            complement(3660..>4329)
                     /locus_tag="VFT03_03220"
     CDS             complement(3660..>4329)
                     /locus_tag="VFT03_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4847183.1"
                     /translation="AREIRKYAREPGMVGVFLPCAGLERLWGHRQYDPIYEAAQEADL
                     PVLLHSVTVIHPVFPFNTQGFETEFGRHICSHTFSMMANLVHMITTGVPVRYPNLRVA
                     VTEAGISWVPFLMHKMDKEYVERRREVPFLEDKPSTYLKKMYFATQPVEEPDNLGDLV
                     TMMNLYDGEDNTIFASDWPHHDFDHPSKVHQVPFTNEVRRKVFGENALRLFNIDASGN
                     RLNL"
BASE COUNT          658 a         1401 c         1436 g          834 t
ORIGIN      
        1 ttttgagacc gtcgagttac tgcaagaggc gctcggccaa gcgcttctac cctactggga
       61 ggatcctgct cgcctacgac agctcgctgg tttctcctgg tgggtggagg ccataaatgc
      121 gttgtgacat caataacccg gatcggtatt actacatcga gacgccgagc agaccgcgcg
      181 tgaactggaa gtcggtgttg cccgccgcga agagccgcgg cgtgtactcc ctggaggcga
      241 cggcggctac ggcgctgagc atctgttctg cccaggagga cgggtcctct tcgaggagca
      301 cgtcgaggtc gtcgccgtgg ctccgtagcg tcccggggtc ggaggaggcc tggaggagtg
      361 ggatcatgcg gtgcgcctgc aaggggcgcc cagcgacatg ggccgccatg atctctaccc
      421 ctgtcgcccc gaggcccgtc gccgtctcga cccagtggtt cgtcggagcc tccatgacgt
      481 gaaagcccgg ctcgcgcact gcttgcccgt aagccacggt gttcttcacc acaagatccc
      541 cgagtacgcg ttccagatat accggagagg agagcatagc cgccgtctcc gggacgacga
      601 ccgtgcctcc ggcgccgacg acggcccggg tggcgcgcgc caggctcagg gcggcggcgt
      661 cggagagggg gcctgacgcg tggagaccga gcctcagagc gccgagcccg gcgggctcgt
      721 gggtgagctc gccggcggct tcgagggtct cggcgaacca gtcttcgacc ttctcgacga
      781 cgctatctat cccgccgtcg agctggacgc tggcccagcc gaagcgcgtt ggatccaggc
      841 cgcgctctct caggaaattc tcgaagtagt cgttgtgggt cttctcacag ccgtgctcca
      901 ggaggagagc gaaccggacg gtggggttgg cgaggtggcc gaggacggtg cgggagtaga
      961 tcctctccgc cgagccggcg gagacgccgc agccctcggt gtgggggaga gcgacgaaac
     1021 gggttacctt gcctcgcgtg gcttcccgct cgttgaggcg gtcggcgatg cggcgcgcga
     1081 tctggcccga gcacaggctc gtgggcatga cgagcccgat ctgatccgcg accggtccgc
     1141 gttcggacgc gatcgcctgg aaggagaact cgagctcatc gtcgccgggc ttcaccggca
     1201 ggggctcgcc gtcaggctcg ggggcgttac ggagctcttc caggttctcg gcgctagtct
     1261 gcttccagtc gcgccagatc gagacctggg agtgtccggc cctctcgccg acggtctttt
     1321 cgcccgaggc ggcgcgcagg gtgcgctcga acatgtcgcg acccagctct tccatcgggg
     1381 tgccgtcgag gtaggcgccg gcgttaacgt ccatgtccct ggagaggagg tcgtagcggc
     1441 cggtcgtggt gacgaccttt atggtgggga cgaaggggaa gttggtgatc gagccgttgc
     1501 cggtggtgaa gaagatcatg ctcgctccgg cggcgacctg cccggcgacg ctctccaggt
     1561 cgttgccggg gctgtccatg aagtagaacc cgccctcgcc catcctctcg gcgtaatcta
     1621 tgacgtggtc gatgcgcacg tcaggatctt tcttcctcgc ggcgccgatg gactttatgg
     1681 agatgttgta gagcccgcgg taattgttcc cgccggaggg gttgtcttca gccgtgtggc
     1741 cgtgccagcc tacccgctcc ttgaaccgct cgaccgtacg gaggaatcgg cgagcggtgt
     1801 ccagatccct cacatgccgc agtacgtagc gctcggcccc gatgagctcg tccgtctcgg
     1861 ccaggttcgc cgcgccgccg ttgcgcacga tctccttcga tacccacccg acgagcgggt
     1921 tcgccgagac cccagagaag gcgtccgatc ccccgcactg caagcctatc ttcagctccg
     1981 aaacaggctc ctccgtgcgc cgcgccccgt ttacctcctc caaccacccg ctcaccacgg
     2041 acttggcccg ctccaggtcg gcccggaagt tgccttcgag gctcatgaac tcgtggggca
     2101 cgtcttctat ggggtagctg ttatcctcca ggtaggagcg gagcatctcg ttggtgacgg
     2161 cctcctggcc tccgtggtcg agggaaagca cagctcccac gttggggttg accatgaacc
     2221 cgctcaaggt gcgcagcagg aggtccaggt tgttcggcgt gcggtcctcg ccgccttcgg
     2281 tgtgggctac gcagactatg ccgctcacgt tctcgcaggc gtcggcgaag cctttcatct
     2341 cccactccag cgccctcacg aaccctgtga gccgcgaggt gacgcccatg acgacgacgt
     2401 agttgcgcgt cccgacgcca cggcccgcac cgcgccggta acccatgaag gagcgcggct
     2461 cgtcgtaggg tggaacctga gcacgaggtc tgaaggtttc ctcgtccagg acgtaggcct
     2521 cgagctcggc gtcgcggaag ttgggctggt cggggagatc cagccctgct ctgcggtatc
     2581 ctcccggaag cctgcccccg ccctggtcgt gggtcccttc ggggtcttcg tccccgtcga
     2641 gacttgggga ggtcccgtgg cgctcgcgca acacgcgcag gatcttctcg ttgcacgcgt
     2701 agtcgccggg ctcgatgtcc ttcgttgctt gcccgaacgt gaggccccac gagaggaggt
     2761 cctcaccctc gccgatccgc tccacggcga accggtgtcc ctcgaggacg gtgtggggca
     2821 ccgtgaactc cgagcgctcg tgcgctaccc gcgtgcctgc ttcgatcctg cgcgtggcta
     2881 tcgccacgtt gtcccctgac gcgggcagac gtccaacttc gtcgaacgtg agccgttcgc
     2941 taccggcggc catgaaagat caccctcccg tcgttcctga taaggctcgt cttcgcagcc
     3001 agtgtatcgc agcgtgagcc ggctctacgg atggtagctc gctggccggg tccctggagg
     3061 tgtgggcggc ccggctgact tctgggagag gcgccggttg tagacgcgga ccatgttctc
     3121 ggcccactcg cgcgccggga aatcgccgct tttctcgaag cgcacgagcc agtcgtccgg
     3181 gtcttcggct cgcacgacca ggaacgccgc ctcctgttcg cgtacggcga gtctcccttc
     3241 tacctgtggt tcgatgccat ccatcccttt cagacgagca ccttcacctt gccgctttcg
     3301 acgactacct cgtagcggcg caggtttatg ttcgggtagg ccaggcaccg cccggtcggc
     3361 acgtggtact ccaggccgtg ccaggggcac acgacgacct cgccgtcgtg gacccactcg
     3421 aagcgccagt cgttctccgc cctggagacg agcgtgccca gaggcggctt gccctcgcag
     3481 agggggcccg tctggtgcgg gcagacgttg gggaggccgt agagcacgcc gccgatgttg
     3541 aacaccccga tctggcgcct cccgatcctc acgaccttct ggcttccctc cgggaagtcc
     3601 gagacgtcgc cgacgacgtg cgcttcgcgt ctcttctcgc cctgcgtcat acgtttccct
     3661 cagaggttca gacggttgcc gctggcgtcg atgttgaaga gcctgagcgc gttctcgccg
     3721 aagaccttgc gccggacctc gttcgtgaag ggcacctggt gtaccttcga tgggtggtcg
     3781 aagtcgtggt gcggccagtc ggaggcgaag attgtgttgt cctcgccgtc gtagaggttc
     3841 atcatcgtca ccaggtcgcc gaggttgtcg ggctcctcga ccggctgggt tgcgaagtac
     3901 atcttcttga ggtatgtgct cggcttgtct tcgaggaacg gcacctcgcg ccggcgctcg
     3961 acgtactcct tatccatctt gtgcatcagg aaagggaccc aggagattcc ggcctcggtg
     4021 acggcgacgc gcaggttggg gtaacggacg gggacgccgg ttgtgatcat atgcaccagg
     4081 ttggccatca tagagaaggt gtggctgcag atgtgccgcc cgaactcggt ctcgaaccct
     4141 tgagtgttga aagggaagac ggggtggatg accgtaacgc tgtgaaggag caccggaagg
     4201 tcggcctctt gggccgcttc gtagatcggg tcgtactgtc tgtggcccca gagccgctcg
     4261 agccccgcgc aaggcaagaa tactccgacc atccctggtt ctcttgcgta cttgcggatc
     4321 tcacgcgcc
//
LOCUS       DASTUV010000156         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_913348, whole genome shotgun sequence.
ACCESSION   DASTUV010000156 DASTUV010000000
VERSION     DASTUV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913348"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFT03_03225"
     CDS             complement(<1..592)
                     /locus_tag="VFT03_03225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU4847184.1"
                     /translation="MKIGVKIPNDSAKAHEIGLTNMGRLAEDAGADSLWVNDHLLMVE
                     GGRSTYPFTPDGAIPWPMDLPRYECLTAASFLAATTSRCRIGTSVLILPQRNVLEFAK
                     TTATLDALSGGRFVLGIGVGWFAEEMEALGYRFETRGQRTDEMIEVLRGCWTGRPRPF
                     RGKEIVVPEGLVFEPRPSQPNSPPILLGGMSRAALRR"
     gene            complement(894..1352)
                     /locus_tag="VFT03_03230"
     CDS             complement(894..1352)
                     /locus_tag="VFT03_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4847185.1"
                     /translation="MRDNSPRPHHLNLAVGGDVARTADFYRNLLGLTPVELPRDQDSY
                     GAAIATLQDRWGYQYHLIPADPGFAARHGLPVNPLGSGHLAFRVDDIAAVRERLDAEG
                     IPYSDMGEWAIKGWHQLFCADPDGRVIEFHQVLDGGADGGTGDTPGQTSS"
     gene            1616..2896
                     /locus_tag="VFT03_03235"
     CDS             1616..2896
                     /locus_tag="VFT03_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit
                     family protein"
                     /protein_id="HEU4847186.1"
                     /translation="MSSERIVATYLIETPHPVEHAAEVVAGEQSSGTFVELPGETDTL
                     QQRHGARIERIEELETVNLPSLPGVRYTPAPGEKVRYRRAEVEISIPLEKVGSSLPAL
                     LATVAGNIYEVAEVSALRLLDLDLPRAFGEAYPGPQFGIEGTKELSGVPADRPVIGTI
                     VKPSVGLTPEQTAELARDLAEVGIDFIKDDELIANPPYSPLERRVEEVMRVVNEVAER
                     TGRKLMYAFNITDDLEAMLRHHDTVLRAGGTCVMVGINNVGLAAFSYLRERCQLPIHA
                     HRNGWGALARYPYLGVEFRAYQKIWRLAGADQLHVNGIQNKFWESDDSVVESIKSCLE
                     PMFDHRTIMPVISSGQWGGQAPETYRRTKSVDLMYLAGGGIVAHPGGPAAGVTAIMQA
                     WDAARNGIPLEEHAREHPELRAAMEKFGSLTPSR"
     gene            2918..4300
                     /locus_tag="VFT03_03240"
     CDS             2918..4300
                     /locus_tag="VFT03_03240"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU4847187.1"
                     /translation="MNQLLLAFYGDDFTGSNDAMESLARLGIRTILFVEPPGPEQLRR
                     FEGIQAVGVAGVSRSLPTAEMDAELLPVFKRFRELNAPVFHYKVCSTFDSSPEIGSIG
                     HAIDLGQETFASPFVPLLVGVPELWRYCVFGNLFARSGPESDVYRLDRHPTMRYHPTT
                     PMTESDLRLHLSRQTTKKIGLFDILQVAAPKGEVEERFAELLGSSPEIVLFDVLYKEQ
                     LSIIGRLIWEHANQGDAPLFVVGSSGMGYALTAHWEAIGLLPDSELPPAAGEVEQLVA
                     ISGSCSPVAARQISRAIEEGFAEIPLQPARLVDPNEAEEEIEVAVEEALRVLGTGRSL
                     VLHTCRGPDDPRIEATVRRLAAVRPRALGAGRSTAKIFGEALGQILREVLEKTGIRRA
                     AIAGGDTSGYATRRMGIEALEVKAPFAPAMPLSRAHAAGTPLDGLEVVFKGGQTGKVN
                     FFEGVRRGKP"
     gene            4326..>4771
                     /locus_tag="VFT03_03245"
     CDS             4326..>4771
                     /locus_tag="VFT03_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU4847188.1"
                     /translation="MQNTTTIAVFGAGGNMGTRVTNALKDEPGYRMLNVEKSEQGRTR
                     LRERGLEISDQEDALREADVVVLAVPDALIGEVATDIVPDLKSGAMLICLDPAAPYAG
                     KLPDREDVSYFVTHPAHPPVYNDESDPEARRDFFGSGKAKQAIVNAL"
BASE COUNT          880 a         1453 c         1588 g          850 t
ORIGIN      
        1 cgcgcctaag ggcggcccgg ctcatcccgc cgaggaggat gggagggctg ttcggttggg
       61 aaggtcgcgg ctcgaacact agaccctccg gcacgacgat ctccttaccc ctgaagggcc
      121 ggggtctccc cgtccagcac cctctcagga cctcgatcat ctcgtcggta cgttgcccac
      181 gagtctcgaa ccggtagcct agcgcctcca tctcctcggc gaaccacccg acgccgatcc
      241 cgagtacgaa gcgacctccg ctcaaggcgt ccagggtcgc ggttgtcttc gcaaattcca
      301 agacgttgcg ctgtggcagg ataaggaccg aggtaccgat cctgcacctc gaggtggtgg
      361 cggccaggaa agaagcggct gtaaggcatt cgtacctggg cagatccata ggccaaggga
      421 tagcgccgtc tggggtgaac gggtacgtcg agcgcccacc ctcgaccatc aggaggtgat
      481 cgtttaccca gaggctgtcc gccccggcgt cttctgccag ccgccccatg ttggtaaggc
      541 ctatctcgtg ggccttggcg ctatcattgg ggatcttcac accgatcttc atagttgcct
      601 cacgtgttgg ctgcccgccg actacttgcg ctgaccgaga tgcccattct actcctctca
      661 tggttggcgg gactccgata ttcgagggca gagtgtcgcc tgccgaggcc ttctcatgga
      721 agttcgcatc caggaatccg catacgttcc cccgagtaca gggggacgca ataggcgcga
      781 ggattgccta gttgcctaga ctagagcctc cgtaggtccg ggtagggcga tctagcggcg
      841 acgctcgcgg cggattttcc tgccagcttg actccgacca tacgccgggc atttcatgac
      901 gaggtttgcc ccggtgtgtc ccccgtcccg ccatcagcac caccatcgag cacctggtgg
      961 aactcgatca cccggccatc ggggtcggcg cagaagagct gatgccagcc cttgatcgcc
     1021 cactcgccca tgtcgctgta ggggatgccc tcggcgtcga ggcgctcccg caccgccgcg
     1081 atatcgtcca cgcgaaaggc gaggtgtccg gagccgagcg ggtttaccgg cagcccgtga
     1141 cgcgcggcga agccggggtc tgcggggatt aagtggtact ggtagcccca gcggtcctgc
     1201 aacgtcgcga tcgcggcgcc gtaggaatcc tggtcgcgcg gcagctcgac gggggtcagc
     1261 cccagcaggt tgcggtagaa gtcggccgtg cgggcgacgt cgccgcccac cgccaggttg
     1321 aggtggtgcg gtcgtgggga attgtctctc attctgcctc ctccggctcg aagaacatct
     1381 caggttcgga ggtgaccccg gcggcgccga agccgccgtc gagctccttt ccgccccgca
     1441 ccgaggttga cgatgtgcgg cagcatatta cagcggtggg gtggcggcaa gatatggtgg
     1501 gaggcacctg ttgtcgggac gtcggcccag atcgagaaga gggcttgcac agcaaggaaa
     1561 tcggccttga gataccatgg tgccggagtg gcttagggaa atgagggtgt agttcatgag
     1621 tagcgaacgg atcgtagcga cgtacctgat cgagaccccg catcctgtgg agcatgctgc
     1681 agaagttgtt gcgggtgaac agtcctccgg caccttcgtc gagctcccgg gggagacgga
     1741 taccctgcag cagcgtcacg gtgcgcgaat agagcgcata gaagaactgg aaacggtgaa
     1801 cttgccgagc ttgcccggcg tccgctacac ccctgccccg ggcgagaagg tgcgctaccg
     1861 gcgcgccgag gttgagatct ccatccccct ggagaaagtc ggcagcagcc tgcccgccct
     1921 gctcgcgacg gtggcgggga acatctacga ggtggccgag gtctccgcgc tgcggctgct
     1981 cgacctagat ttgccgcgag cgtttgggga agcttacccg ggaccgcagt tcggaatcga
     2041 ggggacgaag gagctctcgg gagtgcccgc cgaccgtccc gtcatcggga ccatagtcaa
     2101 gccgagcgta ggactaactc cggagcaaac cgccgagcta gcgcgtgatt tggccgaggt
     2161 tggcatcgac ttcatcaagg atgacgagct aatcgccaac ccgccttact cgccgctgga
     2221 gcgtagagtc gaggaggtaa tgcgggtagt caacgaggta gccgagagaa caggaagaaa
     2281 gttgatgtac gccttcaaca tcaccgacga tctggaggcg atgctccgtc accacgacac
     2341 cgtgttgcgc gccggcggta cctgcgtgat ggtgggcatc aacaacgtgg gactagcagc
     2401 gttttcgtac ctgcgagagc gttgccagct gccgatccac gcgcaccgca acggctgggg
     2461 cgcgctcgcc cgttaccctt atttaggtgt ggagttcagg gcctaccaga agatctggcg
     2521 gctcgcgggg gcggaccagc tccacgtcaa cggcatccag aacaagttct gggagtccga
     2581 cgactccgtg gtggagtcca taaaatcttg cctggagccg atgttcgatc acaggacgat
     2641 catgccggtc atctcgtctg gtcagtgggg cgggcaggct ccggaaacgt accgtcggac
     2701 taaaagtgtg gacctgatgt accttgccgg tggcggtatc gtggcccatc ctggaggacc
     2761 ggcggccggt gtgacggcca tcatgcaggc ctgggatgcc gcaaggaacg gtatccctct
     2821 cgaagagcac gctcgcgagc acccggagct gcgggcagcg atggagaagt ttggctcact
     2881 gaccccctcg cgatagaagg ggatccagat tagcgacgtg aatcaacttc tcctggcctt
     2941 ctacggcgac gacttcaccg ggtccaacga cgcgatggag tccctcgccc gcctggggat
     3001 tcgtacgatc ctgtttgtcg agccgcccgg cccggagcag ctccggcgct tcgagggcat
     3061 ccaggctgtc ggggtcgctg gggtgagcag gtcactgccg acggcggaga tggacgcgga
     3121 actgctgccc gtcttcaagc gattccggga gctgaacgcg ccggtcttcc actacaaagt
     3181 ctgctcgacc ttcgattctt cgccggagat cggcagcatt gggcatgcca tagacctcgg
     3241 gcaggagact ttcgcttctc ctttcgtccc cctcttggtg ggcgtgccgg agctgtggcg
     3301 ctactgcgtc ttcggcaacc tgttcgcccg ctccggcccg gagagcgacg tctaccggct
     3361 ggaccggcac ccgactatgc gttatcaccc taccaccccc atgaccgaga gcgacctgag
     3421 attgcacctg tcgcgacaga cgacaaagaa aatcgggttg ttcgacatcc tgcaggtcgc
     3481 ggcccccaag ggcgaggtgg aggagcgttt tgcagagttg ctggggtcca gccccgagat
     3541 agtgctcttc gacgtgctct acaaggagca gctgtccatc attgggcgct tgatctggga
     3601 acatgccaac cagggagacg cgccgctgtt cgtggtgggc tcttcgggga tggggtacgc
     3661 cctcacagcc cactgggagg ccattgggct gctgccggat tctgagctcc cgcccgccgc
     3721 tggggaggtc gagcagctgg tcgcgatctc tgggagctgc tcgccagtgg cagcgcgtca
     3781 gatctcccgg gccatcgagg aaggatttgc cgagatcccg ctccagcctg cccggctggt
     3841 ggacccgaat gaggcagaag aggaaatcga ggtcgccgtg gaggaggctt taagagtttt
     3901 gggtaccgga cgcagcctag tgttgcacac ctgccgcggc ccggacgatc ccaggatcga
     3961 ggccaccgtc cgtcgcctcg cggccgtgag gcccagggcg ctgggtgccg ggcgcagcac
     4021 cgccaaaatc ttcggcgagg cattggggca gattttgcgg gaagtattgg agaagacggg
     4081 tatccggcgg gccgccatcg ccggcgggga cacctcgggc tacgcgacgc gcaggatggg
     4141 aatcgaggcg ctggaggtga aggcaccctt cgcgcccgca atgcccctgt cccgggcgca
     4201 cgccgccggc acgcccctgg acggtttgga ggtggtcttc aagggcgggc agacggggaa
     4261 ggtgaacttc ttcgagggcg taagacgtgg taagccgtaa atacacgaga gaggagacat
     4321 aaacaatgca aaacacgaca acgatcgcag tcttcggcgc gggtggcaac atgggtacgc
     4381 gcgtaaccaa cgcgctcaag gatgaaccgg gctatcggat gctcaacgtg gagaagagcg
     4441 agcagggacg gacccgcctg cgcgagcggg ggctcgagat ctccgatcaa gaggatgctt
     4501 tgcgagaagc cgacgtggtg gtcttggccg tcccggacgc tctgatcggg gaggtggcca
     4561 ctgatatcgt gcccgacctc aagagcggag cgatgttgat ctgccttgac cctgcggctc
     4621 cctacgccgg aaagctgccc gatcgggagg atgtctctta cttcgtgaca catccggccc
     4681 acccgccagt ctacaacgac gagagcgacc ccgaggcccg gcgggacttc ttcggcagcg
     4741 gcaaggccaa gcaggccatc gtcaacgcgc t
//
LOCUS       DASTUV010000157         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1607062, whole genome shotgun sequence.
ACCESSION   DASTUV010000157 DASTUV010000000
VERSION     DASTUV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607062"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFT03_03250"
     CDS             <1..343
                     /locus_tag="VFT03_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847189.1"
                     /translation="LDFAGITRLWKLLTGKDLSLEPEAQGPPINSDNEVVKAGAFRFT
                     RHPGNLGALGFFLFLPRMTTNRAVLTALVAFYVVLGSLHEEYRLRAAYGAAYERYRRS
                     VPFLIPRPPQK"
     gene            445..666
                     /locus_tag="VFT03_03255"
     CDS             445..666
                     /locus_tag="VFT03_03255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4847190.1"
                     /translation="MSGFSVVIPTLNEERHLGPLLSDVAAQTMSPDQVLVVDAGSDDA
                     TVTVAGASPSSRSLRRSRPWPWAGTRAGG"
     gene            792..1133
                     /locus_tag="VFT03_03260"
     CDS             792..1133
                     /locus_tag="VFT03_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847191.1"
                     /translation="MERFHDLFNLLTRAFEGALPSGAGHCLAVRREIFRASEGFDPAL
                     KFDDIELIRHLSKGCRFGVVEEPVYVSDRCYREGGFARTVAQYSLMALLFALGLYRWA
                     NLIDYEVGVHD"
     gene            complement(1484..2038)
                     /locus_tag="VFT03_03265"
     CDS             complement(1484..2038)
                     /locus_tag="VFT03_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847192.1"
                     /translation="MTERGIDRVERAPEEARQEWLGMLQTLAEQIQKQQQTSQQMVQE
                     MMDTYMQLLNTPGSYVSGQAEQQQQTLQQTAQQWMEQAQQQQQTFQQQAQKQQQAFQQ
                     MTQEVLNTYSQLFSIPLSYAQEGLRSAQFPIEGYDELTVEEVSENLDSLSGEELRVVR
                     DHEERTKNRDTVLEQLDRRIRGGS"
     gene            complement(2194..>2905)
                     /locus_tag="VFT03_03270"
     CDS             complement(2194..>2905)
                     /locus_tag="VFT03_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4847193.1"
                     /translation="DVAALMGVKETTVWRWCREGRLPCLKVGKHWRIRRKVLEDFLER
                     SERSNTLVGQLGSFLRVPDNVLVIAQNIDILHRLDAAFFGVGEARGGLLVKYYAGEIH
                     SEDQLRADFENNGLDVRRLEREGRFFMRPEEDPLDGREEALGRFVEERAGEGRTVWAS
                     FDWVVKVDLDTALERQERLAEIVDARHLVVKTAALAEVIDGWSSAELRRAQSSHSAMI
                     LATDGGLSLSRATPMPPS"
BASE COUNT          438 a         1016 c          869 g          582 t
ORIGIN      
        1 tctcgatttc gccggtataa cgcggctgtg gaaactcctc acggggaagg atctcagcct
       61 cgagccggaa gctcaggggc cgccgataaa ctccgataac gaggtggtga aagcaggggc
      121 gttccgcttc acccgccatc cgggcaacct gggagctttg ggtttcttct tgttcttgcc
      181 tcgcatgacg acgaaccgcg ccgtcctcac ggcgctggtg gctttttacg tagtgctggg
      241 ttccttgcac gaggaatata ggttgcgggc ggcctacggc gcagcctacg agcgctaccg
      301 gagatctgtg ccgttcctga tccctcgccc tccgcaaaaa taggggcgtc atgatgcctc
      361 ccgcaaccct gggcacaggt gtcgcccgac attcgggacg taggttagat tgcgcaggac
      421 ttggcgcccg cggcgaggag cgtggtgagc gggtttagcg tcgtgatccc tacgttgaac
      481 gaggaacgcc acctcggccc cttgctctcc gatgtggccg cgcagaccat gagcccggac
      541 caggtgctcg tggtcgacgc gggctccgac gacgcgacgg tgaccgtcgc cggggcttcc
      601 ccttcgtcgc gctccttgag gcggagccgc ccgtggccct gggcaggaac gcgggcgggc
      661 ggctagcggc gacgtcttgt tcttcgtcga cgccgacgtc cggttgcccg ggcggttctt
      721 cgagggcttt ctcgtggaat tcgaacgacg tcgcctgccc cctgtaccgt ccctacgact
      781 cgacgccggc cgtggagcgc ttccacgacc tcttcaacct gctcacgcgg gccttcgagg
      841 gcgcgctgcc ctccggcgct gggcactgcc tcgcggtcag gcgcgagatc ttccgggcga
      901 gcgaaggctt cgacccggcc ctcaagttcg acgacataga gctgatccgg cacctctcca
      961 aagggtgccg cttcggcgtc gtcgaggagc cggtctacgt ctccgaccgc tgctacaggg
     1021 agggcggctt cgcgcgcacg gtggcgcagt actcgctgat ggcgctcctg ttcgccctgg
     1081 gcctctaccg gtgggctaac ctcatagact acgaggttgg cgtccacgac taacgctgag
     1141 gcaggcgccg agcgcactcg gccgcacccg gggagacgtc gagccgctag tacgcccgcc
     1201 cgctgcgacc ccattggcat cgcccggcgg gccggtcctc tcggaatggc acctccggct
     1261 acccgtcgta cccatgaccg gagtacacca cgcctcgacc gatgccatcc cgcactccgc
     1321 ccgtcttcct cggcccactc tctttttccc tattcacccg gcctcataga agggctgttc
     1381 tctaagaagt cgcacgagat cagtaaccac ctgggctctg gaccctgccg gatctcggcc
     1441 ttctttgtcc gccttccctt ccttccccta ttcccttagg tccttaagag ccgcccctaa
     1501 tcctacgatc gagctgctcc aggaccgtgt cgcggttctt ggttcgctcc tcgtggtcgc
     1561 gcaccacccg caactcctcg ccagagaggc tatcgaggtt ctcggaaact tcctccacgg
     1621 tcagctcgtc gtagccctcg atggggaact gggccgagcg cagcccctct tgggcgtagg
     1681 acagcgggat gctgaacagc tgcgagtagg tgttcagcac ctcctgggtc atctgctgga
     1741 acgcctgctg ttgcttctgg gcctgctgct ggaaggtctg ctgctgttgc tgggcctgct
     1801 ccatccattg ctgggccgtc tgctggaggg tctgctgctg ctgctcggcc tggccggaaa
     1861 cgtaggaacc cggggtgttc aggagctgca tgtaggtgtc catcatctcc tggaccatct
     1921 gctgggaggt ctgctgctgc ttctggatct gctcggcaag ggtttgcagc atgcccagcc
     1981 actcttgcct ggcctcttcc ggcgcccgct ctaccctgtc tatgcctctt tcggtcacat
     2041 cttctcctta ctcgatcagc tttccaggtg ctattgtgta cccttcttgg gaccgccgga
     2101 accctattca cccaacctac tagaggttag ggttttcgga agctcgcacg tagaaggcta
     2161 cgcaccccgc ggtggcccgc ggcgtgttgt atctcaggac ggcggcatcg gcgtagcgcg
     2221 gctcagcgac agcccgccgt cggtggccaa gatcatggcc gagtgcgagg actgcgcccg
     2281 ccttagctcc gccgacgacc acccgtcgat aacctccgcg agcgcggccg tcttgaccac
     2341 cagatgccgg gcgtcgacga tctcggccaa cctctcctgc cgctctaggg ccgtgtccag
     2401 gtcgaccttc acgacccagt cgaaggaggc ccataccgtc cgcccctcac cggccctctc
     2461 ctcgacgaac cgaccgagcg cctcttcccg cccgtcaagg ggatcctcct cgggcctcat
     2521 gaagaaacgt ccctcccgct ccaggcgccg aacatccagg ccgttgttct cgaagtcggc
     2581 gcgcagctgg tcttcggagt ggatctcccc ggcgtagtac ttcaccagca acccgcccct
     2641 cgcctcgccg acgccgaaga aggcagcgtc caggcggtgc aggatgtcga tgttctgggc
     2701 gatcacgagc acgttgtctg gcacccgcag gaaggagccc aactgcccta ccaacgtgtt
     2761 tgagcgctcg ctccgctcga gaaagtcttc cagaactttc cgccggatgc gccagtgctt
     2821 gcccaccttc aggcacggca ggcgtccctc gcggcaccag cgccacaccg tggtctcctt
     2881 gacccccatc agcgcggcga catcc
//
LOCUS       DASTUV010000158         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1379251, whole genome shotgun sequence.
ACCESSION   DASTUV010000158 DASTUV010000000
VERSION     DASTUV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379251"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFT03_03275"
     CDS             <1..272
                     /locus_tag="VFT03_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847194.1"
                     /translation="KARGYERVRRYAGGLEDWEEAGYPLKGEFFGIDITAEMVKEVKK
                     SKGFLPAAAGRDVQGSPNKAEHPGFMTERAGPVMSSNQLRSSEGR"
     gene            275..964
                     /locus_tag="VFT03_03280"
     CDS             275..964
                     /locus_tag="VFT03_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4847195.1"
                     /translation="MRLIEARELTKVYGRGENRVEALTGMNMNVEPGEWIAIMGPSGS
                     GKSTLMNLIGLLDRPTSGSYTLDGRDVSRLRDSELARARRDLIGFVFQSYNLLPRESA
                     LKNVELPMVYAGVGRKERRKRALGALETVGLAERAHHRPPELSGGQMQRVAIARALVN
                     GPALLLADEPTGNLDSVSGAEILDLFEELNASGVTLVVVTHDAEVAGRTQRVVELRDG
                     RIVADEPSEVV"
     gene            964..2145
                     /locus_tag="VFT03_03285"
     CDS             964..2145
                     /locus_tag="VFT03_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847196.1"
                     /translation="MGKMLRIAGMGLSGLMVNRGRSALSMLGIVIGVFAVVLLTSLGN
                     GVQEAITGKLTGVGSNLLLISPGNSEAQGDGPLAARSASTLTREDVEKVRSLPTVAAA
                     SASVPTVALVGTKTIPMTGVDPSYAQIRSVDLAAGRFVEHSGEVVLETEVAKDLLKSG
                     PKDAVGKTLEIRGKNYEVVGVLEVVEPAFGPPVPETSYMITDDALTLSGAGNVGQIVA
                     EARSAGSVDRAAKEIGARLKSAHGGTEDFNVETQKDLLSVFTLATDLLTILLGGIASI
                     SLLVGGIGIMNIMLVSVAERTREIGVRKAIGATDTDVLFQFLFEALLLTLIGGLAGIG
                     LGVGVSALLPEINPIIPTSVTGTSIVLAFGVSALVGLVFGVLPAYRSARLEPVEALRR
                     E"
     gene            2159..>2550
                     /locus_tag="VFT03_03290"
     CDS             2159..>2550
                     /locus_tag="VFT03_03290"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4847197.1"
                     /translation="MELRQRVTAAVRAQFGHPTGFWGRAAGLLMAHRTSNRRRNAWAV
                     SLLDVRPEDRVLEIGFGPGIAIRELGRLARKGYVCGIDHSELMLRRAKRLNAEGLRRG
                     VVDLRLGSVPEDLPAFDEPFDKILAVNAT"
BASE COUNT          457 a          794 c          900 g          399 t
ORIGIN      
        1 tgaaggcccg aggttacgag cgcgtgaggc gttacgccgg cggacttgaa gactgggaag
       61 aggcgggata ccccctgaag ggcgagttct tcggcataga catcactgcg gagatggtta
      121 aagaggtaaa gaaaagtaaa gggttcctac cagccgcggc tggtagggac gttcaaggaa
      181 gtcccaacaa agctgagcat cccgggttta tgaccgaacg agcagggcct gtcatgagct
      241 cgaaccagct caggtcttcc gaggggcgtt gagaatgcgc ctgatcgaag ccagagaact
      301 cacgaaggtg tacggtcggg gagagaatcg ggtggaggca ctgaccggga tgaacatgaa
      361 cgtcgagccc ggcgagtgga tcgccataat ggggccgtca gggagcggaa agagcaccct
      421 gatgaacctc atcggccttc tggaccggcc gacgtccggg tcctacacgc tcgacgggcg
      481 cgacgtgtct cggctgcggg acagtgagct ggcgcgcgcc cgcagggatc tcatcgggtt
      541 cgtcttccag agctacaacc tccttcccag agagagcgcg ctcaagaacg tggagctacc
      601 gatggtctac gcaggcgtgg gcaggaagga gcgacgtaag cgggctctcg gggctctgga
      661 gacggtcggg ctcgccgaga gggcgcacca ccgcccgccc gagctctccg gcgggcagat
      721 gcagcgggta gctatcgcca gagcgctggt caacggcccc gccctgctgc tggccgacga
      781 gccgaccggc aacctggact cggtctccgg cgccgagatc ctcgacctgt ttgaggaact
      841 aaacgcctcg ggggtcacgc tcgtggtcgt cacgcacgat gcggaggtcg ccgggcggac
      901 gcaaagagta gtggagctcc gcgacgggcg gatcgtcgct gacgagccat cggaggtggt
      961 gtgatgggaa agatgttacg gatcgccggg atgggcctct cggggcttat ggtcaaccgg
     1021 gggcgcagcg ccctgagcat gctcgggatc gtcatcgggg tcttcgccgt cgtcctgctc
     1081 acgtctctgg gcaacggggt gcaggaggcg atcaccggca agcttaccgg cgtcgggtcc
     1141 aacctgctcc tcatcagccc gggaaactcc gaggcgcagg gcgacggccc gctcgccgcc
     1201 cgctccgcga gcaccctcac ccgggaggac gtagagaagg tccggagcct gcccaccgtc
     1261 gccgcggcct cggccagcgt ccccaccgta gcgctggttg gcacaaagac catcccgatg
     1321 accggggtgg acccctccta cgcacagata cgctctgtgg acctcgcggc cgggcgcttc
     1381 gtggagcact ccggtgaggt cgtcctggag accgaggtgg cgaaagacct cctgaagtcc
     1441 ggcccgaaag atgccgtcgg caagaccctg gagataaggg gcaagaacta tgaggtcgtc
     1501 ggcgtgctcg aggtggtgga gccggcgttc ggcccgccgg tgcccgagac ctcgtacatg
     1561 atcacggacg acgccctcac gctctcggga gccgggaacg tggggcagat agtcgccgag
     1621 gccaggagcg caggctccgt ggaccgggcg gcgaaggaga tcggggccag gctaaagagc
     1681 gcccacggcg gtacggagga cttcaacgtc gagacccaga aggacctcct ctccgtcttc
     1741 accctggcca cggacctcct caccatcctc ctcggtggca tagcgagcat ctccttgctg
     1801 gtcggcggca tcggaatcat gaacatcatg ctcgtctccg tcgccgaacg caccagggag
     1861 atcggggtcc gcaaggcgat cggagcaacg gacaccgacg tgctgtttca gtttctcttc
     1921 gaggcgctgc tcctgaccct gatcggtggg ctggcgggca tcggcctcgg cgtcggggtc
     1981 tcggcactgc tgcccgagat caacccgatc atccccacct cggtgaccgg tacgtccatc
     2041 gtgctggcct tcggggtgtc ggcgctggtg gggctcgtct tcggggtcct gccggcctac
     2101 cgttcggcac ggctagaacc cgtcgaagcc ctgaggaggg agtaaaggag tcaagcccgt
     2161 ggagctgaga cagagggtca ccgccgccgt ccgcgcccag ttcgggcacc caaccggctt
     2221 ctgggggcgc gcagcgggcc tgctcatggc ccaccgcacg tcgaaccgca ggcggaacgc
     2281 gtgggcggtg tccctgctcg acgtgcggcc ggaggatcgc gtgctggaga tcggctttgg
     2341 accgggcatc gcgataaggg agcttggtcg cctcgcgcgg aaggggtacg tgtgcgggat
     2401 cgaccactcc gagctgatgc tgcggcgggc gaagaggctc aatgccgagg gcctcaggcg
     2461 cggagtggtc gacctgcggc tcggctccgt ccccgaggac ctgcccgcct tcgacgagcc
     2521 gttcgacaag atcctcgccg tcaacgcgac
//
LOCUS       DASTUV010000159         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_92119, whole genome shotgun sequence.
ACCESSION   DASTUV010000159 DASTUV010000000
VERSION     DASTUV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92119"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFT03_03295"
     CDS             complement(<1..315)
                     /locus_tag="VFT03_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEU4847198.1"
                     /translation="MRLIVAEKPSVGRDLASALGRHRKEKGALVGDGWTVTWALGHLA
                     ELAPPDAYGEQYKKWRLESLPILPERFKVRVNRKTRDQFSVVKKLLRDPSVTEVVNAC
                     DAG"
     gene            complement(374..589)
                     /locus_tag="VFT03_03300"
                     /pseudo
     CDS             complement(374..589)
                     /locus_tag="VFT03_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            complement(680..1348)
                     /locus_tag="VFT03_03305"
     CDS             complement(680..1348)
                     /locus_tag="VFT03_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU4847199.1"
                     /translation="MNEHLQLRDLLGPYVMGALGPEEEREVEAHLEECPSCREEVADL
                     RLAHERLTDLVNMEESPPPELKDRLLTGMPRRETRRVPLVAAAAILCALAVLGVLYYT
                     GFFAPDEVASANLQATDLAPKAGGELRVREEDPNARAELEVWGLPQPGKDEYYELWFG
                     KEGGRMSAGTFTVDARGRETLTMTVPEKASSYDQVGITIEQFPKEPRMSAAKPVLIGE
                     LEES"
     gene            complement(1356..1901)
                     /locus_tag="VFT03_03310"
     CDS             complement(1356..1901)
                     /locus_tag="VFT03_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4847200.1"
                     /translation="MRDVSDEVLVGRVAMGGEERALSELYDRYAGLIYGAGIRYLGDR
                     ALAEDLVQDVFTAVWRNASGFDPARASFAAWIYRITRNRATDLIRRRRARVRTVSSDP
                     PFEPGEEDATGELSRSFDLASALSRISPVHREVLTLAYFHGLSQSEISRRTDTPLGTV
                     KSRTTAALRALRESMLEDEGG"
     gene            2137..3150
                     /locus_tag="VFT03_03315"
     CDS             2137..3150
                     /locus_tag="VFT03_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4847201.1"
                     /translation="MTSDLSKEELQSVARRPARGSLKNFLRDLRELASIRALKATVQE
                     FQRDDTLGIAAQLAYYLILALFPFILVLVSLLGTFGSEELASEVLGYFQQVMPEQAYE
                     IIKDFTNNIISGKAEAPGLFTFGILFTIWAASGAFAAFINALNRTYDVQETRPFWKVR
                     GIAILMTLGLSVLILIGVLLLVLGPQIGEAIANVFGLERTFLVVWEVARWPVALFFMV
                     FTVALLYYFAPDVDQPFRWITPGGLIGVVLWVLASVGFNFYVSNFGSYNKTYGSIGAV
                     IVLLLYLYISSLTILFGATLNATLVRMKEEISGSQILDAKPANEKPDILDEETIEEIQ
                     DND"
     gene            3188..3277
                     /locus_tag="VFT03_03320"
     tRNA            3188..3277
                     /locus_tag="VFT03_03320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3222..3224,aa:Ser,seq:gct)
     gene            complement(3389..3559)
                     /locus_tag="VFT03_03325"
     CDS             complement(3389..3559)
                     /locus_tag="VFT03_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4847202.1"
                     /translation="MRHTCATLLRSRGLHPKLVQELLGHASVATTLDRYSHVLPGMGY
                     QTAAVSMEAALS"
BASE COUNT          638 a         1196 c         1105 g          695 t
ORIGIN      
        1 ccccgcgtcg caggcgttta cgacctcggt cacggaaggg tcgcgcaaca gcttcttcac
       61 caccgagaac tggtcgcggg tcttccggtt caccctgacc ttgaaccgct ccggcagaat
      121 gggcaaactc tcgagccgcc acttcttgta ctgctcgccg taggcgtcgg gcggggcgag
      181 ctcggcaagg tgaccgagcg cccaggtgac ggtccagccg tcgccaacca gggcgccctt
      241 ctccttgcgg tgccttccca gggcgctcgc aaggtcacgc ccgaccgaag gcttctcggc
      301 gacgatgagc ctcatacagg cgagtatacg ggagcgagac gggaaatcag cggcgaatac
      361 gctgtccggt gagctaagag gctctgcggg ccagctcctt ctcgatccgc tctccgtcga
      421 acccgacgat ccacttgctc ccgatcttga taaccggcac gccagtctgt ccggtcttgc
      481 gaacgatgtc ccgcgccgcc tccgggtcac gttctatgtt gatctccttg aagggaaccc
      541 cgcgatcttt gaagtatcgc ttcgcccttc ggcaccagga gcacgttgac gtgctaaaaa
      601 gaagaccttg ggcatatcgt gacctccgcg tcgttgtcct tccacttctt gtcgtcggcg
      661 agctaccctc cgggggtgct caggattcct cgagctcgcc tatgagcacc ggtttcgcgg
      721 cgctcatgcg cggttctttg gggaattgct cgatagtgat gcccacctga tcgtagctgc
      781 tagctttctc gggcacggtc atagtgaggg tctcccggcc ccgggcgtcg accgtgaagg
      841 taccagcgct catgcgcccc ccctctttcc cgaaccacag ctcgtagtat tcgtccttcc
      901 ctggctgagg cagcccccag acctccagct cggcccgggc gttggggtct tcttcgcgca
      961 cgcgcagctc gcctcctgct ttgggcgcga ggtcggtagc ctggaggttg gccgaggcga
     1021 cctcgtcggg cgcgaagaac ccggtgtagt agagcacgcc gagcacggcg agcgcgcaga
     1081 ggatggcagc cgcagcaacg agcgggaccc ggcgcgtctc ccggcgcggc atccccgtca
     1141 gaagtcggtc cttgagctcc ggcggcggcg actcctccat gttgacgagg tccgtcaggc
     1201 gttcgtgggc gaggcggagg tcagcgacct cctccctgca cgacgggcac tcttcgaggt
     1261 gcgcctcgac ctcgcgttcc tcctcagggc cgagagcgcc catgacgtaa ggcccgagga
     1321 ggtctctcag ttgcaggtgt tcgttcaccg ccgcgtcacc cgccctcgtc ctcgagcatg
     1381 ctctcgcgca gcgcgcgcag cgcggctgtc gtccggctct tgaccgtccc cagaggtgtg
     1441 tcggtgcggc gggagatctc gctctgcgag agcccgtgga agtacgcgag ggttagcacc
     1501 tcgcggtgga cgggtgagat ccgggagagg gcggaagcta gatcgaagct gcgcgagagc
     1561 tcgcccgttg cgtcctcctc gcccggctcg aagggagggt cgctgctcac ggtccgaacc
     1621 ctggcgcggc ggcggcggat caggtccgtc gcccgattgc gcgtgatgcg gtatatccag
     1681 gccgcgaagc tcgcccgcgc agggtcgaag cccgaagcgt tccgccacac ggcagtgaac
     1741 acgtcctgca cgaggtcctc cgcgagcgcc cggtccccga gataccgtat accagctccg
     1801 tatatgaggc cggcgtaacg gtcgtacagc tcagagagcg cgcgctcctc cccccccatt
     1861 gccacccgcc ctaccagaac ctcgtccgag acgtctctca ctacgagcct ctcccaacca
     1921 tcgctccaaa tgatatcccg cgttccaccc gcgcgcccga acccgggcac gggtggaccg
     1981 aacctgctgt agatgacgac ttccatacgg aatggtatac gccggcaccg gcctttcgga
     2041 tgattcctcg cagcagatcg aagcgggccg tcttacccac aaccacgcgt aactcgtgtt
     2101 gcgttagaat gatgcaacag ctcggaggga gactgagtga cgtcggatct ttcgaaggaa
     2161 gaactccagt ctgtagcgag gcgacccgcg aggggttcgc tcaagaactt cttgcgcgac
     2221 ctcagggaac tggctagcat ccgggcgctc aaagcaacgg tgcaggagtt ccagagggac
     2281 gacacccttg ggatcgcggc gcagctagcc tactacctga tcctggccct cttcccgttc
     2341 atcctcgtgc ttgtctccct ccttggcacg ttcggaagtg aggagctggc ctcagaggtc
     2401 ctcggttact tccagcaggt catgccggag caggcttacg agatcataaa ggacttcacc
     2461 aacaacatca tcagcgggaa agcggaggcg ccggggctct tcacgttcgg catcctcttc
     2521 acgatctggg ccgcctcagg cgccttcgcc gcctttataa acgccctcaa ccggacatac
     2581 gacgtgcagg agacgagacc gttctggaag gtgcggggca tcgcgatact gatgacgctg
     2641 gggctctccg tcctcatcct cataggcgtc ctgctgctgg tcctcggccc tcagatcggc
     2701 gaggccatcg ccaacgtgtt cgggctcgaa aggactttcc tcgtggtgtg ggaggtagcg
     2761 cgctggcccg tggcgctttt cttcatggtg ttcacggtcg cccttctcta ctacttcgcc
     2821 cccgacgtgg accagccttt ccgctggatc acgcccggcg gcctgatcgg ggtcgtactc
     2881 tgggtgctgg ccagcgtggg cttcaacttc tacgtctcga acttcggctc ctacaacaag
     2941 acctacggtt ccataggagc cgtgatcgtg ctgctcctct acctctacat ctcctcgctc
     3001 acgatcctct tcggcgccac gctgaacgct accctggtca ggatgaagga ggagatctcc
     3061 ggcagccaga tcctcgacgc caagcccgcc aacgagaagc cggacatcct cgacgaggag
     3121 accatcgaag agatccagga taacgactga gggaccgaac ccctttctgc tataatccac
     3181 gacttacgga ggagtggccg agtggttgaa ggcggcaccc tgctaaggtg ttatacctct
     3241 taacggggta tcgagggttc gaatccctcc tcctccgctc cgaaaaccgc tatttgcagg
     3301 gtaaatggcg aggcgatgaa aagagccggg actcctccca gcccttacta caccaacgag
     3361 tacaccaacg ctccccagac gacccttttt aggacagggc ggcctccata gaaacggccg
     3421 cggtctgata ccccatacca ggcaggacgt ggctgtagcg gtccagcgtc gtggccaccg
     3481 aggcgtggcc cagtagctct tgaaccagct tgggatggag gccgcgggag cgcagtagcg
     3541 tggcgcacgt gtggcgcatg tgtagcggag gtcgtgaaag cgtagatccg ctggtaagtg
     3601 ccttcctcga tcggagggtg atgatccgct acct
//
LOCUS       DASTUV010000160         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_542425, whole genome shotgun sequence.
ACCESSION   DASTUV010000160 DASTUV010000000
VERSION     DASTUV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542425"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1686
                     /locus_tag="VFT03_03330"
     CDS             241..1686
                     /locus_tag="VFT03_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEU4847203.1"
                     /translation="MEASNVPAGYSDRLYNEDLAPVTERTWTGYSLFAMWMADVHSIG
                     GYTFAAGLFFLGLAGWEVFLALLVGITIVYFLMNLTGIAGQRHGIPYPVLARLSFGLF
                     GANIPALIRAIIAIFWYGIQTWLASVAVVILLLRIFPGLEEYTHNSILGLSTLGWICF
                     LALWAAQLFVLRHGMETVRRFVDWAGPAIYVAMFALAIWIVIESGGHIDFNLGSKELS
                     AGGALLAFVTAASLVVAYFSTLLLNFCDFSRFSPSERMVKVGNFWGLPVNFTAFSLVT
                     VIVTAGSVAVYGEAITDPVALVAKIPNTLILIVGAVTFAVATVGINIVANFVSPAYDL
                     SNAIPKYIDFRRGGLISAVLALLVTPWNIYNSPVAVNYFLGGLGAFLGPLFGIIVVDY
                     YLVKRGHIDVESLYRAEASSPYWYERGINRHAVLVFVVSAAVAAVIALVPTFSALAPF
                     SWFIGAGLSGALYWATARREERTVPEIAPRG"
     gene            1729..2976
                     /locus_tag="VFT03_03335"
     CDS             1729..2976
                     /locus_tag="VFT03_03335"
                     /inference="COORDINATES: protein motif:HMM:NF006775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEU4847204.1"
                     /translation="MKGTATTVMERCELLGNLSEEPGRLTRPFGSQAMREANDIVSGW
                     MRMAGMTTRFDGIGNLIGRYEGMGNETLVVGSHLDTVRDAGRYDGQLGVMVALACVQQ
                     LHNRGERLPYAVEVVAFADEEGMRFGTTFLGSSVYAGAFDESRLNLEDRNGVTLREAV
                     RAFGGDPGALEGRGKGDLLGYCEVHIEQGPVLEQEDLPVGVVTAINGQTRVRAGFIGK
                     AGHAGTVPMEGRRDALCAAAEFVLEVERAAKAEPGSVATVGEISALPGAVNVIPGEAD
                     HSLDLRHPEDPARERLRDYLERRAREIAASRRCELRWQLRQETPAVPADPELSALLER
                     AVEDSGVSVHRLPSGAGHDAAQMAALTSVAMLFVRCQEGISHNPAESVRREDVGTAIE
                     VMSRFLTLLAQDVRHGASRETSS"
     gene            2973..4355
                     /locus_tag="VFT03_03340"
     CDS             2973..4355
                     /locus_tag="VFT03_03340"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HEU4847205.1"
                     /translation="MSTYDLILRSGNLVTPGGSSQADLAIEDGRIAAITPDLEDTARE
                     EVDASGLHVFPGVIDAHAHFNEPGRTRWEGFATGSRSLAAGGTTAYVEMPLNAYPPTC
                     DAACFDEKLALAEASSLVDFAFYGGLVPGNLGDMEELASRGVAGFKAFMSTVGTLDFH
                     TADDLTLFEGMKEAARLGLPVLVHAENRTITDGLANRAISTLRLDARDYLDSRPVVAE
                     LEAIGRAIILAEEAGCSLHVVHVSTGKGVALVAAARARGVDVTCETCAHYLVLTDEDA
                     EELGSAAKCAPPLRPRQDLEELWERIFEHDVEFVTSDHSPCPPEMKVGDDFFRIWGGI
                     SGCQSLLNVLLDEGYHGRGLPLERVAALTAGNVADRFGFSRKGRLEVGYDADLALVDL
                     DSSFTLRTEDLFYRHKLSPYVGRAFRGKVLRTIVRGTTVFRDDKIISDPAGKLIRPDR
                     QTAAMPQRGV"
     gene            4360..4773
                     /locus_tag="VFT03_03345"
     CDS             4360..4773
                     /locus_tag="VFT03_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEU4847206.1"
                     /translation="MGDVRITAGPFEFMARWEREKSPETCEAFEKLLPYRQKIIHVRW
                     SGESCWIPLGDYDLGVGFEDATSYPQAGEILFYPGGYSETEILFPYYGTHFKSKLGTL
                     AGNHFLTITEGHHNLRALGELCLWEGAQDIVFDRA"
     gene            4852..>5097
                     /locus_tag="VFT03_03350"
     CDS             4852..>5097
                     /locus_tag="VFT03_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEU4847207.1"
                     /translation="MIEVMRFCANVSILFKEAPFLERFGRARNAGFSAVEFWWPSGED
                     LGEVEAAIGDAGLSVALFNFDAGDMPAGDRGLISDPDR"
BASE COUNT          895 a         1622 c         1662 g          918 t
ORIGIN      
        1 aagcacaccg tccgctacag gctccgcagg gtgacggagc taaccgggct cgacgtgacg
       61 aggttcgacg acgcggccca gctctacctg gccgtcaggg cagccgagtt gcgctgaagc
      121 tccgctttgt ccaggagaac aaacgtggcg ggagcctctt tattctcgcg tacatagact
      181 ccgggtcagc ctcgcgacta gtatttgcat ctaaagggtg tcggcaaagg ggaaagggat
      241 atggaagcga gcaacgtacc tgcgggatac agcgaccgtc tctacaacga ggacctcgct
      301 ccggtcacgg agcgcacatg gaccggctac agcctcttcg ccatgtggat ggccgacgta
      361 cacagcatcg gcggctacac gttcgctgcg gggctgttct tcctggggct ggcgggctgg
      421 gaagtgttcc ttgcgctgct cgtcggtatc acgatcgtct acttcctcat gaacctcacg
      481 gggatcgcgg gccagaggca cggcatcccc tatccggttc tggcgcgcct cagtttcggg
      541 ttgttcgggg cgaacatacc ggccctgatc cgggcgatca tagccatctt ctggtacggc
      601 atccagacct ggctagcctc cgtagcggtg gtcatcctgt tgctcaggat atttccgggt
      661 ttggaagaat acacccataa ctcgatcctg ggtctctcga ctctgggttg gatctgcttc
      721 ctcgccctct gggcggcgca gttgttcgtg ttgcgtcacg ggatggagac cgtccggagg
      781 ttcgtggact gggcggggcc ggctatctac gtcgccatgt tcgcgctcgc gatctggatc
      841 gtcatcgagt ctggcggcca catagacttc aaccttggct ccaaggaact ctcagcaggg
      901 ggtgctctcc tggcgttcgt tacagccgct tcactggttg tggcgtactt ctcgacgctg
      961 ctgctgaact tctgcgactt ctcgcgcttc tcaccgtccg agcggatggt caaggtgggc
     1021 aacttctggg ggttgccggt caacttcacg gccttctcgc tggtaaccgt gatcgtcacg
     1081 gctggctcgg tggccgtcta tggggaggcc atcaccgatc cggtagcgct cgtcgcgaag
     1141 atccccaaca ccctcatcct gatagtgggc gccgtgacgt tcgcggtcgc gacggttggg
     1201 atcaacatcg tcgccaactt cgtctcgccc gcctacgacc tctcgaatgc tatccccaag
     1261 tacatagact tcaggcgagg aggtctgatc tcggccgtcc tcgccctgct ggtgacgccc
     1321 tggaacatct acaacagccc cgtggctgtg aactacttcc tgggcggcct cggcgctttc
     1381 ctcggcccgc tctttggcat catcgtggtg gactactatc tcgtgaagcg gggacacatc
     1441 gacgtagaga gtctctaccg ggcggaagcc tcttcaccgt actggtacga gcgagggatc
     1501 aaccgccacg ccgtcctggt attcgtcgtc tcggcggccg tggcggcggt catagccctg
     1561 gtgccgacgt tcagcgccct cgcccccttt tcgtggttca taggtgccgg actctccgga
     1621 gcgctctact gggcgacggc gcgaagggaa gagcgcaccg tcccggagat cgcgccccgc
     1681 ggctagataa taggagcttc aggcacggtc cggcgaggag gacctccgat gaaaggcact
     1741 gcgaccaccg tcatggagcg gtgcgagcta ctgggcaacc tcagcgaaga gcccggccgt
     1801 ctaacccgtc ccttcggttc tcaggcgatg cgagaagcca acgacatcgt ctccggctgg
     1861 atgcgcatgg ccgggatgac cacgagattc gacgggatcg gcaacctcat tggccgctac
     1921 gaggggatgg ggaatgagac gctcgtcgtc ggctcccacc tcgacaccgt gcgcgacgcg
     1981 ggccggtacg acggccagct aggcgtcatg gtcgcactcg cttgcgtcca gcaactgcac
     2041 aaccgcggcg aacgcctccc ctacgccgtt gaggtggtcg ctttcgccga cgaggagggc
     2101 atgcgcttcg gcacgacatt cctgggcagc tctgtgtacg ctggcgcctt cgacgagagc
     2161 cgcctgaacc tcgaagaccg gaacggcgtg accctcaggg aagcggtccg cgcctttggc
     2221 ggagatcccg gcgccctgga aggccgcggg aaaggggacc ttctcggcta ctgcgaggta
     2281 cacatagagc agggaccagt gctggagcag gaggacctcc ccgtaggcgt agtcacggcc
     2341 atcaacggcc agacccgcgt gagggccggc ttcatcggca aagccggaca cgcgggaacc
     2401 gtgccgatgg aaggtaggcg ggacgcgttg tgcgccgccg ccgagttcgt gctcgaggtc
     2461 gagcgcgccg cgaaagcgga gccgggctcg gtcgcgaccg tcggggagat ctccgctctc
     2521 cccggggcgg tcaacgtcat tcccggggag gcggaccact cgctggatct ccgccacccc
     2581 gaagacccgg cgcgagagcg gctgcgcgac tacctcgaac gcagggccag agagatagct
     2641 gcctcccgcc ggtgcgagct ccgctggcaa ctgcgtcagg agacgcccgc cgtgcccgcg
     2701 gacccggagc tgagcgcgtt gctcgagagg gccgtcgagg actcgggcgt atctgttcac
     2761 aggctgccga gcggcgcggg ccacgacgcg gcacagatgg ccgcgctcac cagcgtggcg
     2821 atgctcttcg tgcggtgcca ggagggcata agccacaacc cggcggagtc ggtcaggaga
     2881 gaggatgtgg gaacggccat agaggtgatg agccgctttc tcacgctcct cgcccaggac
     2941 gtgcgccacg gcgcatcccg ggagacgagc tcatgagtac ctacgacctg atcctgagga
     3001 gcggcaacct cgttacgcct ggcggttcct cgcaagccga cctcgccatc gaggatggcc
     3061 gcatcgccgc catcacaccc gacctggagg acacggctcg ggaagaggta gacgcttcgg
     3121 gactccacgt ctttccgggg gtcatcgacg cgcacgcgca cttcaacgag ccgggccgca
     3181 cgcgctggga gggcttcgcc acgggctccc gatctctcgc agccggaggc acgaccgcgt
     3241 acgtggagat gccgctgaac gcctatccgc ccacctgcga cgcggcgtgc ttcgacgaga
     3301 aactcgccct cgcggaggct tcctcgctcg tggacttcgc gttctatggc gggctcgtcc
     3361 ccggcaacct cggggacatg gaagagctcg ctagtagagg agtcgcggga ttcaaagcct
     3421 tcatgtccac cgtcggcacg ctcgacttcc ataccgcaga cgacctgacg ctcttcgagg
     3481 ggatgaagga agcggcccgg ctcgggcttc ccgtcctcgt acacgcagag aacaggacta
     3541 tcacggacgg tcttgccaac cgcgcaataa gcacgctgcg cctggacgcg cgcgactacc
     3601 tcgactcgcg tcccgtcgta gccgagttag aggcgatagg aagggcgatc attctcgccg
     3661 aggaggccgg atgctcgctt cacgtcgtgc acgtgagcac gggtaagggc gtcgccctcg
     3721 tggcggcggc gcgcgccaga ggagttgacg taacctgcga gacctgcgct cactatctcg
     3781 tcctgaccga tgaggatgcc gaagagctcg gctcggctgc caagtgcgcc ccaccgctcc
     3841 gtccgcgcca ggatctcgag gagctctggg aacggatctt cgaacacgat gtcgagttcg
     3901 tcacctcgga tcactcgccc tgtcccccgg aaatgaaggt cggcgacgat ttcttcagga
     3961 tctggggcgg catctccggc tgccagtcgc tgctcaacgt cttgctcgac gagggatacc
     4021 acggacgagg gttgccactg gagcgggtag ccgcgctcac ggcagggaac gtggcggacc
     4081 gtttcgggtt ctcccggaaa ggccgccttg aggtcggcta cgacgcggat ctcgccctcg
     4141 tagatctcga ttcgagcttc accctgcgaa ccgaggacct tttctaccgc cacaagttga
     4201 gcccttacgt gggccgtgcg ttccgcggga aagtcctccg cacgatagta cgtggtacca
     4261 cggtcttccg cgacgacaag atcatctccg acccggccgg caagcttatc agacccgacc
     4321 ggcagaccgc tgcaatgcca cagagaggag tatgaaaacg tgggcgacgt caggatcacc
     4381 gccggacctt tcgagttcat ggcccgctgg gagcgcgaga agtcgcccga gacctgcgag
     4441 gctttcgaga agctgctgcc gtaccgccag aagatcatcc acgtaaggtg gagcggggag
     4501 tcgtgctgga tccctctggg cgactacgac ctcggggtgg gcttcgagga cgccaccagc
     4561 tatccgcagg ccggcgaaat cctcttctat cccggcggct actcggagac ggagatcctc
     4621 ttcccctact acggcacgca cttcaagagc aagctgggca cgctcgccgg caaccacttc
     4681 ctgaccatca ccgaaggcca ccacaacctg cgtgctctgg gcgagctgtg cctctgggag
     4741 ggcgctcagg acatagtttt cgaccgcgcc tgaagagcat atgctccacc ggaggcaagc
     4801 aacggttgcg tttagaatac gggaactggc aagatacgcc acggggagag ggtgatagag
     4861 gtcatgaggt tctgcgcgaa cgtctccatc ctgttcaagg aggcgccgtt tctggagcgg
     4921 ttcgggaggg caaggaatgc cgggttctcg gctgtggagt tctggtggcc ttcgggcgag
     4981 gacctcggcg aggtggaggc ggcgatcggg gacgccggcc tttcggtggc cctgttcaac
     5041 ttcgacgccg gggacatgcc tgctggggac cgcggtctga tcagcgaccc ggaccgg
//
LOCUS       DASTUV010000161         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1429733, whole genome shotgun sequence.
ACCESSION   DASTUV010000161 DASTUV010000000
VERSION     DASTUV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429733"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1601)
                     /locus_tag="VFT03_03355"
     CDS             complement(111..1601)
                     /locus_tag="VFT03_03355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4847208.1"
                     /translation="MTDERTVLERPVARLTVTADAGLLPVVVDFVRRVAHRLGLRDRA
                     AERLDWAVETVCRNVIEHAFEVDEEGRYDVEVLRRPGRVVIAVEDQGLPFDYAPLRDD
                     EDTALPEMLHHSFADEVRFINLGRGGNRVELIKHLPHGDVREELPEEVHHRVRDAPAA
                     PEDVRLEIRMMRPEESAELSRCVYRSYGYSYDWDYVYYPDRIRELQEGGLMRSCVAVT
                     PEGEFVGHLAAKVERPDSPVAEAGQAVVDPRFRGHRLFERMKTFLAQRANEWGMYGLY
                     SEATAVHPYSQRGNLKLGAGETGFLLGYIPASVSYKEIGEDREGRRGSVALIYMRTSD
                     EPERTVYPPQVYRELVRRVVEHNELRRAVEHNPDAEAPPSTRMNVNVRRDHNLAFMQV
                     EEPGADLHELVQARLRELRLHGVDCAYVDLPLSHPATSRAGAGLGDLGLFFGGIIPEA
                     APTGGDVLRLQCLNEVEIKVEDVRTASNFGEELVDLIFQQKRTVER"
     gene            complement(1739..2668)
                     /locus_tag="VFT03_03360"
     CDS             complement(1739..2668)
                     /locus_tag="VFT03_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU4847209.1"
                     /translation="MAFSVFAQVLLPILVVVASGYLLQRRLELDLHSVNRLSLYLLSP
                     ALIFSSLAQSRIEVSETLRIGAFMTLFVVSIGALTWLVAVLLRLDSIDRAALMLCVMF
                     MNAGNYGLPAARFAFGQEGFDRAVLFFVVQAILAQTLAIYIAGAGHGDRRGGLARLLR
                     MPQVYAVLAALAVRLGGLRLDPTGGGILNDVFRGVALVGDAAVPLLLIILGLQLAETG
                     RITEGPRVALATILRLGLSVPLALGLSYLLNLDELSTKLAVMLSSMPTAVNVTILAIE
                     FNIRPRFVSSVVVVSTAASVFTLTLILVLLGVD"
     gene            complement(2676..>3801)
                     /locus_tag="VFT03_03365"
     CDS             complement(2676..>3801)
                     /locus_tag="VFT03_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU4847210.1"
                     /translation="YKDFVPDHDALIVERLRGAGAISVGKTNTPEFGAGSQTFNEVFG
                     ETLNPYDPARTCGGSSGGAAAALACGMVPIADGSDMGGSLRNPASFCNVVGFRPSPGR
                     VPSWPDPTPWLSLTVDGPMARTVEDAALMLSAIAGPDARCPASISEPGTLFLRPLERD
                     FGGVRVAWSRDLGGLPVDPGVTAVLEEHRCVFEALGCAVEEAEPDFAGADEVFKVLRA
                     WRYELAYAELLEDHREEMKDTVVWNIEEGKRLGGSQIGRAERERMEIFHRVRTFMQSY
                     EFLVMPVSQVPPFDVKQRYVTEISGERMKTYIDWMRSCYYVTVTGSPAVSVPCGFTPE
                     GLPVGVQIVGRYRDDFGVLQLAHAFERATRHGERRPPLAG"
BASE COUNT          648 a         1274 c         1251 g          628 t
ORIGIN      
        1 cctgtgcttg ccgacggcac cgtgtgggtg ggataagcac gccgcttcgc ggcttgtcag
       61 cacgctccgg ctgagttctc gaggtgctgc ctcggtatcc gttgggacgg ttagcgttcc
      121 acggtgcgct tctgctggaa gatgaggtcc accagctctt cgccgaaatt cgaggcggta
      181 cggacgtcct cgactttgat ctcgacctcg ttgaggcact ggagcctgag cacgtctccg
      241 ccggttgggg cggcttcggg gatgatgccg ccgaagaaga gaccgagatc tcctaacccg
      301 gccccggcgc gcgaggtggc cgggtgggag agcggcaggt ccacgtaggc gcagtccacg
      361 ccgtgcaggc ggagctccct tagccgggcc tggaccagct cgtgcaggtc ggccccgggc
      421 tcttcgactt gcatgaaggc caggttgtga tccctgcgga cgttgacgtt catccgggtg
      481 gagggcggcg cttccgcatc cgggttgtgc tcgacggcgc ggcgcagctc gttgtgctcg
      541 acgacgcgcc ggaccaattc tctgtatacc tggggtgggt agacggttcg ctccggctcg
      601 tcgctggtgc gcatgtagat cagggcgacg gatccccgcc tcccttccct gtcttcaccg
      661 atctccttgt aagagacgga agccgggata tagccaagca ggaatccggt ctccccggcg
      721 ccgagcttca ggttgcccct ctgactgtag ggatgtacgg cggtggcctc gctgtagagt
      781 ccgtacatac cccactcgtt cgcgcgctgg gcgaggaacg tcttcatcct ctcgaacaac
      841 cggtggccgc ggaagcgtgg gtctacgact gcctgcccgg cctccgcgac cggggagtcg
      901 gggcgctcca ccttcgcggc caggtgcccc acgaactctc cctcgggagt taccgccacg
      961 caggagcgca tcaggccgcc ttcctgtagc tcgcggatcc tgtccgggta gtagacgtag
     1021 tcccagtcgt agctgtagcc gtaagagcgg tacacgcagc gcgacagctc cgccgactcc
     1081 tccggccgca tcatgcggat ctccagcctg acgtcctccg gggcggcggg agcatccctg
     1141 acgcggtggt gcacttcttc gggaagctct tccctgacgt cgccgtgcgg caggtgtttg
     1201 atcagctcta cccggttgcc gccacgcccc aggttgataa agcgtacctc gtcggcgaaa
     1261 gagtggtgga gcatctccgg cagagccgtg tcttcgtcat cccggagggg agcgtaatcg
     1321 aagggcagcc cctggtcctc tacggcgatg acgacccgcc cggggcgccg gaggacctcg
     1381 acgtcgtagc gaccttcctc atcgacctcg aaggcgtgct cgatgacgtt gcggcagacc
     1441 gtctcgactg cccagtccag ccgctcggcc gctctgtccc tcaacccgag ccggtgagcc
     1501 acccgccgca cgaagtccac cacgacaggc agcagccccg cgtcggccgt aaccgtgagg
     1561 cgggcgacgg gtctctccag caccgtacgc tcatccgtca aagacaacac ctcccgtctc
     1621 cgcactcgta ccagaagtca ccgcaaccgc tgacaagcac gtaagccgac catgacatct
     1681 tacggattct gcggagacgc tgccgggcat atgagagagg gacggagaga actacgaatc
     1741 agtctacgcc gaggaggacg aggatcaggg tgagggtgaa cacgctcgcc gccgttgaga
     1801 ccacgacgac gctgctcacg aagcgggggc ggatgttgaa ctcgatcgcg agtatggtca
     1861 cgttgaccgc cgtcggcatg ctcgagagca tcacggcaag cttcgtcgag agctcgtcga
     1921 ggttcaacag gtagctcagg cccagcgcga gggggacgga gagccccagg cgcagtatcg
     1981 tcgccagcgc gactctcggt ccctcggtta tcctccccgt ctcggccagc tggaggccca
     2041 ggatgatcag gagcagcggc acggcggcgt ctcctacgag cgccacgccg cgaaacacgt
     2101 cgttgaggat gccgccgccc gtgggatcga gccttagccc tccgagccgg acggccagcg
     2161 ccgcaaggac cgcgtagacc tggggcatcc tgagcagccg cgcgagcccc cctctccggt
     2221 cgccgtgacc agcccccgcg atgtagatgg cgagcgtctg cgcgaggatg gcctggacga
     2281 cgaagaagag caccgcgcgg tcgaatcctt cctggccgaa ggcgaaccgc gccgctggga
     2341 gcccgtagtt ccccgcgttc atgaacataa cgcagagcat cagggccgcc ctgtctatgg
     2401 agtccagccg taagaggacg gccacgagcc aggtcagcgc gccgatcgag accacgaata
     2461 gcgtcatgaa ggccccgatg cgcagcgtct ccgagacctc gatccgcgac tgcgcgaggg
     2521 aggagaagat gagggccggg ctcaacaggt aaagactcag gcggtttacc gaatggaggt
     2581 cgagctcgag cctgcgctgc agcaggtagc ccgaggcgac gacgaccagg atcggcagca
     2641 ggacctgcgc gaatacggag aaggctatta ccggctcacc ccgcgagcgg cggacggcgc
     2701 tcgccgtggc gcgtggccct ctcgaaggcg tgggctagct gcaagacgcc gaagtcgtcg
     2761 cggtagcggc cgacgatctg gactcccacg ggcagccctt ccggcgtgaa gccgcaaggc
     2821 accgagacgg cgggtgatcc ggtaacggtc acgtagtagc aggacctcat ccagtctatg
     2881 taggtcttca tccgctctcc gctgatctcc gtgacgtagc gctgctttac gtcgaagggt
     2941 gggacctggc tcaccggcat caccaggaac tcgtaggact gcatgaaggt ccgcacccgg
     3001 tggaagatct ccatccgctc gcgttccgcc cttcctatct gcgatccgcc gaggcgcttc
     3061 ccctcctcga tgttccagac cacggtgtcc ttcatctcct cccggtggtc ctctagcagc
     3121 tcagcgtagg cgagctcgta gcgccacgcc ctgagcacct tgaagacctc gtccgcgccg
     3181 gcgaagtcgg gctccgcttc ctccaccgcg caacctagag cctcgaatac gcatcgatgc
     3241 tcctcgagca ccgccgtcac gcctggatct acgggcaggc cgccgaggtc ccggctccag
     3301 gcgacccgga cgccgccgaa gtcgcgttcc agggggcgca ggaacagggt gcccggctcg
     3361 gatatagagg cggggcagcg agcgtccgga ccggctatcg cgctcagcat gagagctgcg
     3421 tcttcgaccg tgcgggccat gggcccatcc accgtgagcg agagccaggg cgtggggtct
     3481 ggccagctcg ggacccggcc cggcgagggc ctgaatccca ccacgttgca gaagctcgcc
     3541 gggttgcgca gggacccgcc catgtcgctg ccgtcggcga taggcaccat cccgcacgcc
     3601 agagccgccg ccgccccgcc gctgctaccg ccacacgtcc tggccgggtc gtaggggttg
     3661 agagtctcgc cgaagacctc gttgaaggtc tgcgagccgg cgccgaactc aggggtgttc
     3721 gtcttgccga cggagatggc tcccgcccct ctcagacgct ccacgatcag ggcgtcgtgg
     3781 tcggggacga agtccttgta g
//
LOCUS       DASTUV010000162         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_630592, whole genome shotgun sequence.
ACCESSION   DASTUV010000162 DASTUV010000000
VERSION     DASTUV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630592"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFT03_03370"
     CDS             <1..551
                     /locus_tag="VFT03_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847211.1"
                     /translation="MFLPFPGWQVLVGFIVSATVLGYAVVPLAFGALRRQEPDHPRPF
                     ELPGGEVTAVLAFIVANLVIYWTGWDIYWKLVVAIVFGFVLLGIGHLVNPSELTPSFD
                     WRGGSWLLPYLVGMGVISYIGASDFGGLGIIPFGWDALVIAAFSVAIYFYAMSVRLTP
                     EEVRRHVADAKQEAEQEEELAI"
     gene            complement(604..1884)
                     /locus_tag="VFT03_03375"
     CDS             complement(604..1884)
                     /locus_tag="VFT03_03375"
                     /inference="COORDINATES: protein motif:HMM:NF005373.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEU4847212.1"
                     /translation="MGELDAELAQAIARVVEESRGESVRLLQELVRIPSVTGDEGAVQ
                     EVVERAFRERGLAMDVWEASPEEISPYKEHVGEQAGYENRPNVVGVRAGKGGGRSLLL
                     NAHVDTVAAGDASAWSRDPFSGDVEGDLLYGRGSCDMKGGLVSHLAALDALSELGVEL
                     RGDVSVAATVGEENGGLGALSTVLRGYRADAALITEPTRLALVPAQGGSLVFRLEVPG
                     RSAHAAVRDEGVSALEKFIPIFEELGKLEDERNATLRHPLYERVRNKVPINVGVVRAG
                     NWASTVPESLVAEGRVGLIPGEEIETFRETVAERIAAVARRDPWLRENPPKLEWFGGQ
                     FAPAEVPPDAPICEAVKRAHERAASEEPIVEGVPYGADMRLFIRFGGMPCVMYGAGDV
                     NVAHAPDEYVSITELLTASKTVACLLAEWCGVAA"
BASE COUNT          303 a          739 c          633 g          358 t
ORIGIN      
        1 tcatgttcct gccgttcccg ggatggcagg tgctcgtcgg tttcatcgtc tcggcgaccg
       61 tgttggggta cgccgtggtg ccgctcgcct tcggcgcact gaggcggcag gagcccgacc
      121 acccgcggcc attcgagctg ccaggcggcg aggtgacggc ggtcctcgcc ttcatcgtgg
      181 cgaacctggt catctactgg accggctggg atatctactg gaagctggtc gtcgctatcg
      241 tcttcggctt cgtgctcctc ggcattgggc acctggtcaa cccctcggag ttgaccccct
      301 ccttcgactg gaggggaggc tcgtggttgt taccctacct cgtggggatg ggcgtgatct
      361 cctacatcgg cgcatcagat ttcggcggtc tagggatcat cccgttcggc tgggatgccc
      421 tcgtcatagc ggccttcagc gtcgccatct acttttacgc catgtcggtg cgtcttacgc
      481 ccgaagaggt gcgacgccac gtcgccgacg cgaaacagga ggcggaacag gaagaagagc
      541 tcgcgatata acgctctggg ggctacggga gagctggctc tcccgtagcc cccagcctgc
      601 ggatcatgcg gccacgccgc accactctgc cagcaggcac gcgacggttt tactcgccgt
      661 caacaactcc gtgatgctca cgtattcgtc cggggcgtgc gcgacgttga cgtctccggc
      721 gccgtacatg acacagggca tcccgccgaa gcggatgaac agccgcatgt ccgcgccgta
      781 ggggacaccc tcgacgatgg gctcctcgct ggcggcccgc tcgtgggctc gttttacggc
      841 ttcgcagatc ggggcgtcgg gcggcacctc cgccggggcg aactggccgc cgaaccactc
      901 tagcttcggg gggttctctc ttagccacgg gtcgcgcctg gcgacggcgg cgatgcgctc
      961 cgcgaccgtc tcgcggaagg tttcgatctc ctcgccgggg atcaggccta cccgcccctc
     1021 cgcgacgagc gactccggga cggtagaggc ccagttgcct gcgcgcacca caccgacgtt
     1081 gataggcacc ttgttgcgca ctctctcgta cagcgggtga cgcagagtag cgttgcgttc
     1141 gtcctcgagc ttccctagct cctcgaagat cggtatgaac ttctccagtg ccgagacgcc
     1201 ttcgtcgcga acggcagcgt gggcggagcg gccggggacc tcgagcctga acacgagcga
     1261 gccgccctgg gccggcacga gcgcgaggcg ggtgggctcg gtgatcaggg ccgcgtcagc
     1321 cctgtatccc cgcaagacgg tcgagagggc acccagcccc ccattctcct cccccaccgt
     1381 cgccgccacg ctgacgtccc cgcgcagctc gaccccgagc tccgagagcg cgtcgagcgc
     1441 ggccaggtgg ctgacgagcc cacccttcat atcgcacgag ccacggccgt agagcaggtc
     1501 gccctcgaca tctccggaga acgggtcccg gctccaggcc gacgcgtcac cggccgccac
     1561 cgtgtccacg tgcgcgttca gaaggagcga gcgcccgcct cccttgccgg cgcgcacccc
     1621 gactacgttc ggacggttct catagccggc ctgctcgcca acgtgctcct tgtaggggga
     1681 gatctcctcc gggctcgcct cccagacgtc catggcgagc ccgcgctccc tgaaggcgcg
     1741 ctcgactacc tcctgcaccg cgccctcatc ccccgtgacg gaagggatgc ggaccagttc
     1801 ttgcagcagc cgcaccgact cgccgcgact ctcctccact acccgggcta tcgcctgcgc
     1861 cagctcggcg tcgagctccc ccacggctgc gtcctcctct gcgcgtggat ccagttcggc
     1921 aacgcgccgt ctcctgcgcg gcacgagaac ctgaaacgag ccaaccggtg cccgtgatcc
     1981 ccttccccac tgaccccctc acggacaccg gtaatattcc cgacataccc gct
//
LOCUS       DASTUV010000163         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_44984, whole genome shotgun sequence.
ACCESSION   DASTUV010000163 DASTUV010000000
VERSION     DASTUV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44984"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /gene="rplE"
                     /locus_tag="VFT03_03380"
     CDS             <1..97
                     /gene="rplE"
                     /locus_tag="VFT03_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4847213.1"
                     /translation="RGLDVAVVTTTESDEEARELLRLLGMPFRDS"
     gene            108..293
                     /locus_tag="VFT03_03385"
     CDS             108..293
                     /locus_tag="VFT03_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU4847214.1"
                     /translation="MTRKALLVKQQKKQKYPVREYNRCQRCGRAHGFYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            300..698
                     /gene="rpsH"
                     /locus_tag="VFT03_03390"
     CDS             300..698
                     /gene="rpsH"
                     /locus_tag="VFT03_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4847215.1"
                     /translation="MSVNDPIADFLTRIRNAQMAKHESAEIPHSKMKADIARILKEEG
                     YIKDFSEKGAGAEKVLVIELKYGPDGQRAIAGLRRMSRPGRRVYRKQKDIPRVLDGLG
                     VAILSTSQGILTDHAARRQGIGGEVLCFVY"
     gene            711..1244
                     /gene="rplF"
                     /locus_tag="VFT03_03395"
     CDS             711..1244
                     /gene="rplF"
                     /locus_tag="VFT03_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU4847216.1"
                     /translation="MSRIGKAPVEIPGGVNAEISGRSVKVTGPRGELTVPVGRGVEVR
                     QEDGSLVLERASDAPEHKAMHGLTRSLIQNAVTGVTEGFSKTLQIAGVGYRAALQGRD
                     VNLQVGYSHPVLIHPREGVEFEVPNATTIVVRGIDKQRVGQMAAEIRKVRPPEPYKGK
                     GIRYSDEQIRRKVGKAG"
     gene            1267..1620
                     /gene="rplR"
                     /locus_tag="VFT03_03400"
     CDS             1267..1620
                     /gene="rplR"
                     /locus_tag="VFT03_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU4847217.1"
                     /translation="MTVVEKKRVHREKRHKRVRRKVSGTAERPRLSVYRSNVHIYAQL
                     VDDDAANTLAAADSREVGEAENRKEAARKVGELIANRATDAGIEAVVFDRGGNKYHGR
                     IAALAEGARSGGLKL"
     gene            1634..2224
                     /gene="rpsE"
                     /locus_tag="VFT03_03405"
     CDS             1634..2224
                     /gene="rpsE"
                     /locus_tag="VFT03_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU4847218.1"
                     /translation="MGRPRTGQNRGQGPAGGNPRGGGGPGRDRGGPRGRERGGRDGDL
                     QDRVVEIRRVAKVVKGGRRFNFSALVVVGDGNGRVGVGLGKANTVPAAIGKGQDRAKA
                     NMFAVPMRNTTIPHEVLGRFESSSVMLKPASEGTGVIAGGAVRAVMELAGVRDVLTKA
                     LGSTTAVNLVQATVDGLKQLRSKADIEKTRGVKITQ"
     gene            2221..2409
                     /gene="rpmD"
                     /locus_tag="VFT03_03410"
     CDS             2221..2409
                     /gene="rpmD"
                     /locus_tag="VFT03_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU4847219.1"
                     /translation="MSSLKVTQVKSVIGSKGDHKRTVRALGLKRIRDSRVHEDTPQIR
                     GMLHKVRHLVHVEEVEAR"
     gene            2406..>2748
                     /gene="rplO"
                     /locus_tag="VFT03_03415"
     CDS             2406..>2748
                     /gene="rplO"
                     /locus_tag="VFT03_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU4847220.1"
                     /translation="MRLNELKGAPGSKKARKRVGRGTGSGHGKTSGRGHKGAGARSGS
                     KAHSTYEGGQMPLQRRLPRLKGEARGRHTVARPTLYAPVNLGQLGDISGDTIGPEALR
                     AAGLVRKKAELV"
BASE COUNT          561 a          810 c         1015 g          362 t
ORIGIN      
        1 ccgcggcctc gacgttgcgg tggtgacgac gacggagagc gacgaggagg cgcgggagtt
       61 gttgcggctg ctcgggatgc cgttcaggga cagttgagag gtagggtatg acgagaaagg
      121 ctttgctggt aaagcagcag aaaaagcaga aatacccggt acgggagtac aaccggtgcc
      181 aacggtgcgg ccgggcgcac gggttctacc gcaagttcgg gctctgcagg atctgcctca
      241 gggagctcgc ccacgagggc aagatccccg gagtaacgaa agcgagttgg taggaaaaga
      301 tgtcggttaa cgatcccata gcggacttcc tcaccaggat ccgcaacgcg caaatggcga
      361 agcacgaatc agccgagatc ccccactcca aaatgaaggc ggacatagcc cgcatcctca
      421 aggaggaggg ctatatcaag gacttctcgg agaagggcgc tggggccgag aaggtcctcg
      481 ttatcgagct caagtacggc cccgacgggc agcgcgcgat tgcgggcctg aggcggatga
      541 gccgccccgg caggcgcgtc taccgcaagc agaaggacat cccccgggtg ctcgatggtc
      601 tcggggtggc gattctctcg acctcgcagg gaatcctcac cgatcacgcg gcacgacgcc
      661 aggggatcgg tggcgaggtc ctctgcttcg tgtactaggg aggttcgaga atgtcacgga
      721 taggaaaggc accggtcgag atcccgggcg gtgtgaacgc cgagatctcg ggccggagcg
      781 tgaaggtaac cgggcctaga ggcgagctga ccgttcccgt cgggcgcggc gtcgaggtca
      841 ggcaggagga cgggagcctc gtgctggagc gggcgtcgga cgcccccgaa cacaaggcga
      901 tgcacggtct tacccgtagc ctgatccaga acgccgtgac cggcgtgacg gagggtttct
      961 cgaagacctt gcagatcgcg ggcgtcggct accgggccgc gctgcagggg cgcgacgtga
     1021 acctgcaggt cgggtactcg catccggtcc tgatccaccc gcgcgagggt gtggagttcg
     1081 aggtcccgaa cgcgactacc atcgtcgtgc gcggtataga caagcagcgc gtcggccaga
     1141 tggcggcgga gatccgcaag gtgaggccgc ccgagcccta caagggcaag ggtatccgct
     1201 acagcgacga gcagatccgg cgcaaggtcg gcaaggccgg ctagcggggt tcgaggaggt
     1261 ttcaggatga cggtagtcga gaagaaacgg gtgcacaggg agaagcgcca caagcgcgtg
     1321 cggcgcaagg tgtcggggac ggcggagcgc ccccggctct cggtgtacag gtcgaacgtg
     1381 cacatctacg cccagctcgt cgacgacgac gcggccaaca ctctagctgc ggccgactcc
     1441 cgcgaggtgg gcgaggccga gaaccgcaag gaggcggcgc gcaaggtcgg cgagctgatc
     1501 gcgaaccgcg ccacggacgc cggtatagag gccgtggtct tcgaccgcgg cggcaacaag
     1561 tatcacggcc gcatcgcggc gctcgcagag ggcgcgcgct caggcggatt gaagctttag
     1621 gaggtaacag atcttgggac gaccgaggac aggtcaaaac agaggccagg gtcctgccgg
     1681 cggcaacccc cgcgggggtg ggggccccgg tagggaccgc ggagggccta gggggcgcga
     1741 gcgcggcggg cgcgacggcg acctccagga cagggtggta gagatccggc gcgtggcgaa
     1801 ggtcgtcaag ggcggccggc gcttcaactt cagcgccctg gtagtcgtcg gcgacggcaa
     1861 tggcagggta ggcgtcggtc tcggcaaggc caacaccgtg ccagcggcca taggcaaggg
     1921 ccaggaccgc gcaaaagcca acatgttcgc agtgcccatg cgcaacacca cgataccgca
     1981 cgaggtgctc ggcaggttcg agagctcctc ggtcatgctc aagccagcct cggagggtac
     2041 gggcgtgatc gcaggcggcg ccgtccgggc ggtcatggag ctggccggcg tcagggacgt
     2101 gctcaccaaa gccctcgggt cgacgaccgc ggtgaacctg gttcaggcta cggtagacgg
     2161 cctcaagcag ctgcgctcga aggcggacat cgagaagacg cggggggtga agatcaccca
     2221 atgagctccc tcaaggtaac ccaggtaaag agcgtcatag gctcgaaggg agatcacaag
     2281 cggacggtgc gcgctctggg gctcaagcgc atccgggact ccagggtgca cgaggacacg
     2341 ccccagatcc gaggcatgct ccacaaggtg cgccacctcg tgcacgtaga ggaggtcgaa
     2401 gcacggtgag gttgaacgag ctcaagggtg ctcccggctc gaagaaggcg cgcaagcgcg
     2461 tcgggcgcgg cacggggagc ggacacggca agacgagcgg ccgcggccac aagggcgcgg
     2521 gcgcccgttc cggatcgaag gctcactcca cctacgaggg cggccagatg ccactgcagc
     2581 gcaggttgcc tcgcctcaag ggagaggcgc gcggcaggca cacggtcgca cgcccgacgc
     2641 tgtatgcacc cgtgaacctg ggccaactcg gggacatctc cggtgatacg ataggccctg
     2701 aagcgctgcg agccgccggc ctggtgagga agaaggccga gctcgtca
//
LOCUS       DASTUV010000164         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_116554, whole genome shotgun sequence.
ACCESSION   DASTUV010000164 DASTUV010000000
VERSION     DASTUV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116554"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFT03_03420"
     CDS             complement(<1..186)
                     /locus_tag="VFT03_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847221.1"
                     /translation="MARYRMAAESLGVEMGQVRLVAAHSRDVAGALRAGCAAAFVARP
                     GMVLDPLVERPDVVGVDL"
     gene            complement(205..624)
                     /locus_tag="VFT03_03425"
     CDS             complement(205..624)
                     /locus_tag="VFT03_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU4847222.1"
                     /translation="MHQNPGTEEIKDLLAGSRTVAVVGLSDRPHRTSHSIARALRDFG
                     FRIFPVNPNLRGPVLGEEPYATVEEVPEAVDIVDVFRSSEKVMPTARDAVAAGAKVLW
                     MQSGVINEEAAAYAREHGLKVVMDRCIMVDYASLVGQ"
     gene            746..2098
                     /locus_tag="VFT03_03430"
     CDS             746..2098
                     /locus_tag="VFT03_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEU4847223.1"
                     /translation="MRGALATDPSDLAVQAAEVLRRNDMGEWTRASPTLYPHQWSWDT
                     GFIAIGLAHLDTRRAAMELTGLFAHQWRNGKVPHIVFNPDAPPDSYFPGAEHWSSAGT
                     FPDAPPAPPYTSALCQPPTHALGALRVWELAGRDEEGFARGFLREIFPKLLAWHRYLA
                     TYRDPEGSGLVTIYHPWESGTDNSPRWDAALEAVEVGDLPSYERQDLNHVDHPDERPT
                     DEEYVRFLWLVELIKRARCDEGALNDSHPFLVKDVLFSAILVAANEALVEIARVVGAS
                     QEDVALIEGWIGRGREGLEERWDQELGLCLDYDLRRDAPLRARTIAGFAPLVAGGIEA
                     GEMEAVLRTLYSPYFLGYPKLRRPLPPSTSPEEPRFHPRNYWRGPMWPVMAWLFWRSL
                     ELAGEVERAQAMQRAALQELAEGGFAEYFEPFTGEPLGSDNQSWTAAVALDWLAADPA
                     "
     gene            complement(2170..2373)
                     /locus_tag="VFT03_03435"
     CDS             complement(2170..2373)
                     /locus_tag="VFT03_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEU4847224.1"
                     /translation="MTEISATKPNSFEDAINEGVTRATSTLRNVEGAWVKDQNVLIEN
                     GSIVGYKVNLAVTFVLEDAGDIS"
BASE COUNT          400 a          829 c          766 g          425 t
ORIGIN      
        1 cagatccacg ccgaccacgt caggacgttc caccagcggg tcgagaacca tccccgggcg
       61 cgcgacgaag gccgccgcgc agccggcgcg taaagcccct gccacgtccc tggagtgggc
      121 ggcgacgagg cggacctggc ccatctcgac cccgaggctc tccgcggcca tcctgtacct
      181 cgccatgctc cctacctttc cttgctactg gccgaccagc gacgcgtagt cgaccataat
      241 gcagcggtcc atgacgacct tcaggccatg ctctctggcg taggcggctg cttcctcgtt
      301 aatgacgccg gactgcatcc acaggacctt cgcccccgcc gccacggcgt ccctcgcggt
      361 gggcattacc ttctcgctgc tgcggaacac gtcgacgatg tccaccgctt ccggcacctc
      421 ctcgacggtg gcgtaaggtt cttcgccgag gaccggcccg cgcaggttgg ggtttacggg
      481 gaagatcctg aacccgaagt ccctgagggc ccgcgctata gagtggctgg tacggtgcgg
      541 cctgtccgag agcccgacga ccgcgacggt acgcgagccc gcgagcaggt cttttatctc
      601 ctctgtcccg ggattctggt gcatcgcgtc tccctctcgg aggctatctc gaccggatgt
      661 taaccgatct cgccgcccgg cggcgcacac tctctgcgtc taatcaccag ggaaccggcg
      721 ctcggtctgg tagcataggc gccgtatgcg aggagcgctg gccacggacc cttcagacct
      781 ggctgtgcag gcggcggagg tcctgcgccg caacgacatg ggagagtgga ccagggcctc
      841 gccgaccctc tatccacacc agtggagctg ggacaccggc ttcattgcca tcgggctcgc
      901 gcacctcgac acccgccgcg ccgccatgga gctaaccggc ctgttcgcgc accagtggag
      961 aaacggcaag gtgccccaca tcgtcttcaa cccggacgct cccccggaca gttatttccc
     1021 aggcgccgag cactggtcga gcgcgggaac gttcccggac gccccgcccg cgccaccgta
     1081 caccagcgcc ctgtgccagc cgccgacaca cgccctcggc gccctgcgcg tctgggagct
     1141 ggccgggagg gacgaagaag gcttcgcgcg ggggttcctg cgagagatct tcccgaagct
     1201 cctggcctgg caccgctacc ttgccacata cagggacccg gagggatcgg ggctcgtgac
     1261 catctaccat ccctgggaga gcggcacgga caactctcca aggtgggacg cagccctcga
     1321 ggccgtcgag gttggcgacc tgccgtcgta cgagcgccag gacctgaacc acgtggacca
     1381 tcccgacgag cgtcccacgg atgaggagta tgttcgcttc ctctggctcg tcgagctcat
     1441 aaagcgcgcc cgctgcgacg aaggcgccct caacgactca cacccttttc tggtcaagga
     1501 cgtgctattc agcgcgatcc tggtcgcagc aaacgaggct ctggtagaga tagctcgcgt
     1561 tgttggggct tcccaggagg acgttgcgct aatagaaggc tggatcgggc gcggacgaga
     1621 agggctcgag gagcggtggg atcaggagct gggcctgtgc ctggactacg acctcaggcg
     1681 ggacgcgccg ctgcgggcgc gcaccatcgc gggcttcgcg ccgctcgtgg cgggtggcat
     1741 agaggcggga gagatggagg ccgtgctgcg tacgctctac tcgccgtatt tcctgggata
     1801 cccgaagctg cgccggccgc tgcctccctc cacgagcccc gaggagccgc gcttccaccc
     1861 gcgcaactac tggcgcggtc ctatgtggcc ggtcatggcg tggttgttct ggcgttcgct
     1921 cgagctcgcg ggcgaggtgg agcgcgcgca ggccatgcag cgggcggccc tgcaagagct
     1981 cgccgaagga ggcttcgcag agtatttcga gcccttcact ggcgagccgc tcggctcgga
     2041 caaccagtcc tggaccgcgg ccgtcgctct ggactggctc gcagcggacc cggcgtagag
     2101 atggccttca atacgaaaca ccccggctac gagccggggc gctctggttc cgcgaaagtc
     2161 agctcctaat taactgatgt ccccggcatc ttcgaggacg aaggtgaccg ccaggttcac
     2221 cttgtagccg acgatgctgc cgttctctat caggacgttc tggtccttga cccaggcgcc
     2281 ttctacgttg cgcagcgtgg acgtcgcccg agttacacct tcgttgatgg catcctcgaa
     2341 gctgttcggt ttagttgcgc ttatctcggt cacgcgagca acactcataa atcctcctta
     2401 ttggtagcca aagctctccc
//
LOCUS       DASTUV010000165         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_307115, whole genome shotgun sequence.
ACCESSION   DASTUV010000165 DASTUV010000000
VERSION     DASTUV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307115"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            716..1876
                     /locus_tag="VFT03_03440"
     CDS             716..1876
                     /locus_tag="VFT03_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEU4847225.1"
                     /translation="MDIEIGKGKTARRAYGLDEIAIVPSRRTRDPEDIDISWSLGNLH
                     LDLPCLASALDAAVDPNTAGIIGSLGGLAVLNLEGLQTRYEDPGPVFEEIASLPETKA
                     TRVMQDIYAEPIKEELIFRRVQEIKDRGVIAAASLTPQRVERYHRAAIEAGLDVLVVQ
                     GTVVSAEHVSSKVKPLNLMEFVPSLNVPVIVGGCASYSTALHLMRTGAVGVLVGVGPG
                     RICTTRGVIGVGVPQATAVADAAAARMRHYLETGEYVNVIADGGMRTGGDVARAIACG
                     ADAVMLGSAFAKAEEAPGRGYSWGMATFHPTLPRGTRISTRTNGTLEEILVGPAHEND
                     GTLNLMGALRMSMATTGYQNIKEFQKAEVMYAPSLNTEGKRDQRDQRVGMGG"
     gene            1876..>2417
                     /gene="guaA"
                     /locus_tag="VFT03_03445"
     CDS             1876..>2417
                     /gene="guaA"
                     /locus_tag="VFT03_03445"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU4847226.1"
                     /translation="MILVLDFGGQYAQLIARRVREARVYSELIPYDTPVEVIRRRNPE
                     GIILSGGPSSVYEDGAPKVDGALFDLGVPILGICYGMQLMALALGGEVGAVQIREYGR
                     SDIVVKSHDGLFAGTPDEQKAWTSHGDAVFAVPEGFEITAETPSVPIIAFEERERGRY
                     GVQFHPEVRHTEYGLEILKN"
BASE COUNT          443 a          726 c          810 g          438 t
ORIGIN      
        1 ctatggtctt ttcgaaggcc tcgacttggg acgttccgga gaaattgctt cctttattcg
       61 gtccaaagga acctgtcatt ctgtccttta cctttgctcg ctgcttgatc tcggggccgg
      121 ttcggacgtc tacgttcgtg agaggcgtca ttccgtaacc ctttctgttt cgccggggtc
      181 ccagagggta tatattgcgg ccaagtggcg accgaatagg agggagcgag gcggtgggta
      241 ggggactcta cctgatcatc gctgttctgg tggtgatact cctgatcgtg ctcgcactgt
      301 cgttcgttac gtaaaagggc ttaccgaagg aggcgagaga tgcggggcgg cgttctaaac
      361 ctggtgatcg cggtgctggt gataatcctg ctcgtataca tcatcctgca ggtgatccaa
      421 tagttctggc gatcgggtgg ataatgggag aaaggagaag atggggtgtc gctaaacacg
      481 gtgatctacg tgctgctgat catactggtc gttgtcgtga tactggcgct gctgggagta
      541 gtctagggac cgcgagggcg gcacgcctga cggcttgacc cctgcgagaa ggcgggttag
      601 cataaccgcg atttgcagcg gatagcagag agtattatcg aagcccacgg aggacgcgct
      661 ccgtgggctt cgtcgcgcct gctcggggcg gttagaaagg agtcctgtaa cgtctatgga
      721 tattgagatc ggaaaaggca aaacggcacg gcgggcctac ggcctcgacg agatcgccat
      781 agtccccagc cgcaggactc gcgaccccga ggacatagac atctcctggt cgttgggcaa
      841 cctgcacctg gatctacctt gcctcgccag cgcgctggac gccgccgtgg acccgaatac
      901 ggcgggcatc atagggtctc tagggggcct cgccgtcctc aacctcgagg ggttgcagac
      961 tagatacgag gaccccggtc ccgtcttcga ggagatagcg agcctgcccg agacgaaggc
     1021 tacgcgggtc atgcaggaca tctacgccga gcccatcaag gaggagttga tcttccgccg
     1081 cgtccaggag ataaaggata ggggcgtcat agctgctgca tccctcacgc cccagcgggt
     1141 cgagcgctac caccgggccg ccatagaggc gggcctcgac gtgctcgtcg tgcaggggac
     1201 ggtggtctcg gccgagcacg tctcgagcaa ggtcaagccc ctcaacctga tggagttcgt
     1261 cccgtcgttg aacgttcccg tcatcgtcgg cggctgcgcg agttactcga cggccctcca
     1321 cctcatgcgc acgggcgccg tgggggttct ggtcggcgtg ggtcctggcc gcatctgcac
     1381 tacgcgcggc gtgatcgggg tcggcgtgcc gcaggcgacc gcggtcgccg acgccgccgc
     1441 ggcccggatg cgccactatc tggagaccgg cgagtacgtc aacgtcatcg ccgacggcgg
     1501 gatgcgcacc ggcggggacg tggcccgagc tatcgcttgc ggagccgacg ccgtcatgct
     1561 cgggagcgcg tttgcgaaag ctgaggaggc gccgggccgc gggtactcgt gggggatggc
     1621 gaccttccac cccacccttc cacgcggcac ccgcataagc accaggacga acggcacgct
     1681 cgaggagatc ctggtcggtc cggcccacga gaacgatggg accttgaacc tcatgggcgc
     1741 cctgcggatg agcatggcga ctaccggcta ccagaacatc aaggagttcc agaaggccga
     1801 ggtcatgtac gcgccgtcgc tgaacacgga gggcaagcgc gaccagcgcg accagcgcgt
     1861 cgggatgggc gggtagtgat cctcgtcctg gacttcggcg gccagtacgc tcagctcatc
     1921 gcccgccggg tccgcgaggc ccgcgtctac tccgagctta tcccgtacga cacgcctgtc
     1981 gaggtgataa ggcgtcggaa cccggaaggc atcatcctct ccggtgggcc aagcagcgtc
     2041 tacgaggacg gcgcccccaa agtagacggt gcgctcttcg acctgggcgt acccatcctg
     2101 ggcatctgct acgggatgca gctcatggcg ctggctctgg gcggggaggt gggcgccgtc
     2161 cagatccggg agtacggccg ctccgacata gtagtaaaga gccacgacgg cctcttcgcc
     2221 ggcacaccgg acgagcagaa ggcctggacg agccacggcg acgcggtctt cgccgtcccc
     2281 gagggcttcg agataacagc cgagacgccc tccgtcccga tcatcgcctt cgaggagcgc
     2341 gaaaggggcc gttacggggt tcagttccac cccgaggtgc gccacacgga gtacgggctg
     2401 gagatactca agaactt
//
LOCUS       DASTUV010000166         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_48656, whole genome shotgun sequence.
ACCESSION   DASTUV010000166 DASTUV010000000
VERSION     DASTUV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48656"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /gene="trpD"
                     /locus_tag="VFT03_03450"
     CDS             complement(<1..536)
                     /gene="trpD"
                     /locus_tag="VFT03_03450"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU4847227.1"
                     /translation="MLRQTLRRAVDGEVLTQGEAERALETIMEGNVPPAATAALLTAL
                     RVKGERVPEIVGFARAMRRFAAKVEAPEGVVDTCGTGGDAKGTINVSTAAAFVARGAG
                     VVVAKHGNRAATSRAGSADVLEALGAEIELSPEQVSRCITEVGIGFLFARTHHPAMRF
                     VAPVRAELPFRTVFNLLGP"
     gene            complement(546..1109)
                     /locus_tag="VFT03_03455"
     CDS             complement(546..1109)
                     /locus_tag="VFT03_03455"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU4847228.1"
                     /translation="MRVLVIDNYDSFTYNLVQYLGELGAEVLVRRNDEVTPEEVSALH
                     PDRIVVSPGPCTPNEAGVSVELIEEVGERIPTLGVCLGHQSIAQAFGAEVVRGEPVHG
                     KTARIMHDGRGVYRGLDQGFEATRYHSLVIEPGSVPECLVVTSRTEDGTIMGVRHREY
                     PVEGVQFHPESILTRSGHDLLKNFLGL"
     gene            1161..2111
                     /locus_tag="VFT03_03460"
     CDS             1161..2111
                     /locus_tag="VFT03_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEU4847229.1"
                     /translation="MTLLLTENEVEELLDMPSTLDAVESVLRQHAEGRATNRARRRVA
                     LPTGGLNYMAAGAPEIGALGLKAYTVARTGARFYTMLFDPEGGELLSIMQSDRLGQVR
                     TGAASGVATKYLAREEANTLGIYGAGWQAESQLEAIAAVRDLDRVIVYSRSEESRKEF
                     AEKMGGRLGMEIETTHAAEEPAAQDIVVTVTSSKEPVLLGEWLRPGAHVNAAGSNFLF
                     KTEIDREVVRRASFVCADSREELGLEAGDLMQSLETGAILPEAVYELGQVIAGQVPGR
                     RGPEDITLFASQGLALEDMAAARVVYARAVERDVGRDIAF"
     gene            2253..>2583
                     /locus_tag="VFT03_03465"
     CDS             2253..>2583
                     /locus_tag="VFT03_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847230.1"
                     /translation="MFFANGVVIGTWVVRIPAVQQRLGLGEGLLGVALLGAAVGALAA
                     MPLVGALVSRFGSRRIVGATALLLSLSLLTPALAPNLFLLMAALMLLGAANGGLDVSM
                     NAQAVEVE"
BASE COUNT          432 a          847 c          868 g          436 t
ORIGIN      
        1 ggcccgagca gattgaagac cgtcctgaaa ggcaactccg cccgcacagg ggccacgaac
       61 cgcatcgcgg gatgatgggt tcgggcgaac aagaacccga tgccgacctc ggtgatgcag
      121 cgggagacct gttccgggct cagctcgatc tccgcgccaa gcgcctctag cacgtcggcg
      181 ctaccggccc ggctcgtcgc cgccctgttg ccatgtttgg ctaccaccac gccggcccca
      241 cgcgccacga atgcggccgc cgtggagacg tttatggtcc ccttcgcgtc gccgcccgtg
      301 ccgcaggtgt cgacgacgcc ctcgggggcc tcgaccttcg cggcgaagcg gcgcatggcg
      361 cgtgcgaagc cgacgatctc gggtactctt tcccccttta cgcgcagcgc ggtaaggagt
      421 gcagccgtgg ccgcaggggg aacattaccc tccatgattg tctccagcgc gcgttccgcc
      481 tcgccctggg tcaggacctc cccgtcgacg gctctgcgaa gcgtctggcg tagcacgggg
      541 ccggctcaga gccccaggaa gttcttcagg aggtcgtgac cgcttcgggt caggatgctc
      601 tcggggtgga actggacgcc ctcgacgggg tactcgcggt gcctgacgcc catgatcgta
      661 ccgtcttcgg tgcgactggt gacgaccagg cattccggga cagaacctgg ctcgatgacg
      721 agcgaatggt agcgtgtagc ctcgaagccc tggtccaggc ctctgtagac ccccctgcca
      781 tcgtgcatta tcctggcagt cttgccgtgg acgggctcgc cgcgcactac ctcggcgccg
      841 aaagcctgag cgatggactg gtgtccgagg cagacgccta gagtcgggat gcgctcacct
      901 acctcctcga tcagctcgac cgagacacca gcctcgttcg gcgtgcaggg accgggtgag
      961 actactatcc tatccgggtg gagcgcggac acctcctccg gcgtgacctc gtcgttacgc
     1021 cgcacgagga cctcggcccc gagctctcca aggtactgga ccaggttgta cgtaaaggag
     1081 tcgtagttgt ctataacgag gacccgcata agtggagcga ttttagcgga taagccgccg
     1141 gtgaacaggg aggagacgtt ttgacgttgc tcctgaccga gaacgaagtg gaagagcttc
     1201 tggacatgcc ttcgacgctg gatgccgtcg agtcggtcct gcgccagcac gcagaaggac
     1261 gcgccaccaa ccgcgcccgg cgccgcgtag ccctccctac gggcggactc aactacatgg
     1321 ccgcaggggc gcccgagatc ggggccctcg gactcaaggc atacaccgtc gcgcgcaccg
     1381 gcgcccgttt ctacaccatg ctcttcgacc ccgagggcgg ggagcttctc tctatcatgc
     1441 agtcagacag gctaggacag gtgcgcacag gcgcggcaag cggggtggcg accaagtacc
     1501 tcgcccgaga ggaagcgaac actctaggca tctacggggc cggctggcag gccgagagcc
     1561 agctagaggc catagcggcc gtaagggacc tggaccgggt aatcgtctac agccgcagcg
     1621 aggagtcgcg caaggagttt gcggagaaga tgggcgggcg gctggggatg gagatagaga
     1681 cgacccacgc ggccgaggag cctgcagccc aggacatcgt cgtcaccgtg acgtcatcga
     1741 aggagcccgt gctcctcggc gagtggctca ggccaggggc gcacgtcaac gccgcggggt
     1801 ccaacttcct gttcaagacc gagatcgacc gtgaggttgt aaggcgggca agcttcgtat
     1861 gcgccgactc gcgcgaggaa ctcggtctcg aggcagggga tttgatgcaa tccctggaaa
     1921 ccggcgccat cctgccagag gccgtctacg agctcggcca ggtcatcgcc ggacaggtgc
     1981 cgggccgccg cggccctgag gacataaccc tctttgcaag ccagggcctg gccctggagg
     2041 acatggccgc agcccgcgtg gtctacgctc gcgcggtcga gcgcgacgtg ggcagagata
     2101 ttgcatttta ggacgagacc cgtaggggtg cgtttcaaac ccgcccctac acgcgaaccg
     2161 cccctacgtt cctcgtcgct ggtgcggttg tgagcgatgc gcacgcacat caccggccgg
     2221 cgcttcgcgc gccgcggctc gcggtgctcg gtgtgttttt cgccaacggc gtcgtgatcg
     2281 ggacctgggt cgtcaggata cccgccgtgc agcagcgact aggcctcggc gagggcctcc
     2341 ttggcgtcgc cttgctcggg gctgcggttg gggctctggc cgccatgccg cttgtggggg
     2401 ccctggtctc ccgcttcggg agccggcgca tcgttggtgc gacggcgctc ttgctctccc
     2461 tctcgcttct cacgccggcg ctggcgccga acctgttcct gctcatggcc gccctcatgc
     2521 tgctaggggc ggcgaacgga gggctagacg tctccatgaa cgctcaggcc gtcgaggtcg
     2581 aga
//
LOCUS       DASTUV010000167         5194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_732255, whole genome shotgun sequence.
ACCESSION   DASTUV010000167 DASTUV010000000
VERSION     DASTUV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732255"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFT03_03470"
     CDS             <1..365
                     /locus_tag="VFT03_03470"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEU4847231.1"
                     /translation="GRWGPGYRTQKPVPGQPACVACGSRLVSSVVNFGDPLPQTEYEL
                     AEVHARRCDLMLVLGSSLLVQPAASLVGLAHKSGARIVLINRGKTPYDRAATLRVWTG
                     IGEVIPPAVERTKRALVG"
     gene            564..1283
                     /locus_tag="VFT03_03475"
     CDS             564..1283
                     /locus_tag="VFT03_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU4847232.1"
                     /translation="MSERGGRTRLRDRAAEQLLDMVVSSGLRPGQRLPPERELVGRLG
                     VSRTVVREALNLLEARGLISIEHGRGAVVSGGSTRAVRDTLGLLLRVRPKALWELLEI
                     REILEVEISGLAAERAQEGDVREMRGQLDRMGSLIDAPEGYVDADVEFHALLARSTGN
                     EVLLTMLDPIVELLRASREVSASRPGSARRALREHEEILHCVEAGDAGGARERMRVHL
                     ANTARDIEAALGEDMREMDGV"
     gene            1280..2224
                     /locus_tag="VFT03_03480"
     CDS             1280..2224
                     /locus_tag="VFT03_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU4847233.1"
                     /translation="MKARELLTDLDLPARDLAELPDSPRRFPDGAQYRVEIPSVEGPR
                     VLEAVLDEARKRGVQLHRVSQGSGILLLTDAEILEMCAMARGAGLELSLFVGPRASWE
                     TGAAVVSGAGKVLGAQHRGQDQLAYALEDVLRGVSLGLRGVLVADAGLLWVLRDLKAK
                     GELPEDLVVKVSVQLSAANAAALKAMEDLGADTYNVPTDLSLAQLAAMRAVADLPLDV
                     YVEVPDDFGGFVRHYEVPDLVRVASPVFVKAGLKNAPNIYPSGGHLEATAVALGRERV
                     RRAEIALSMLERYYPEAKTTERGASFPGIPAAKSEVNA"
     gene            2221..3390
                     /locus_tag="VFT03_03485"
     CDS             2221..3390
                     /locus_tag="VFT03_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU4847234.1"
                     /translation="MKITDVETFVVGTDWRNLTIVRLHTDEGLTGLGEARMVNHTDAL
                     VGYLAEAGPRHVVGHDPFRIEELVKKLGRDDFSRAGEVLSSGMAVFETACWDIIGKAL
                     DQPVYNLLGGPVREKVKAYANGWYRVERTPEEFHAAARKVVERGYRALKLDPFGPGHY
                     ELERDEKLRAVALVEAVRDAIGPEPEIMLEMHGRFSPATAVEMAKMMEPFGLGWVEEP
                     VPPVNLKALKKAAEGMPGGIPVATGERIHQRLEYREVFELQAADVIQPDISHIGGLLE
                     TKKLAAWADAYYVTLAPHNVGGQINTAAALHLAACTTNFKIQEYFNDFADPWVRETAP
                     GLPEVVDGYFALPQGPGLGVELDEEVIAAHPKKNVHFNLFAEGWEKRETTRKSDR"
     gene            3387..4172
                     /locus_tag="VFT03_03490"
     CDS             3387..4172
                     /locus_tag="VFT03_03490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEU4847235.1"
                     /translation="MIDLSGKRALVTGAGMGIGQGIAIELARQGADVAVHYAHSEKDA
                     IETASEIERLGVGHALVQGDLSRVVECERVVDEAANSLGGLDVLVNNAGVTRALDFLD
                     TDEATYDEMFDLNMKGYFFCARRAVPLMLEAGGGSIINITSVHGYAGFPRHAAYAATK
                     GAINAFTRELAVELADRGVRVNAIGPGLIEVPRYFDTPGYSTEFGNTLVPSGRIGYPK
                     DVAPTAAFLASDASDFVTGQVIYVDGGTTARMGLWWEQVERPQ"
     gene            4264..>5194
                     /locus_tag="VFT03_03495"
     CDS             4264..>5194
                     /locus_tag="VFT03_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU4847236.1"
                     /translation="MTENGTGSYRLGRRQFLAASALASAGLLLSGCRPEAQQASQGGG
                     GSGGSGGNFPDTPEWNFVFVNHVTTNPFFVPTQYGIEDAQALLGTTSQWTGSETSVVR
                     EMVDAMNSAISGGANGIAVSVVDPEAFNDPIQKALDQGIPVIAYNANGEGPGTNPALA
                     YVGQDLYQSGVEMGNRIVDLVGEGLVALFIATPGQLNIQPRIDGAKQSIEDSGANIEV
                     QQVTTGAELPEERSRIEAWYNGHKDVAGMFAVDAGSTQGVAQVMEQFGLHDKGVKAGG
                     YDLLPEILKLMKAGHIDFTIDQQPYLQGFFPVMQ"
BASE COUNT          943 a         1603 c         1848 g          800 t
ORIGIN      
        1 cgggccggtg ggggccgggc taccgcaccc agaagccggt gcccgggcag ccggcctgcg
       61 tcgcctgtgg cagccggctc gtctcgtcgg tggtgaactt cggagacccg ctgccgcaaa
      121 cggagtacga gctggccgaa gtgcacgcac gtcgctgcga cctgatgctc gttttgggct
      181 cctcgctatt ggttcagccg gcagcctcgc tggtggggtt ggcgcacaag tccggagccc
      241 gaatagtcct gattaaccgg ggcaagacgc cctacgatcg ggcggcgacg ctgcgggtgt
      301 ggacgggtat cggtgaggtg attccgccag cggtggagcg gacgaagcgg gcgctcgtcg
      361 gatagtcgaa gatccaggcc gtaggcaata aacatcccct gcgctgtacg aactgactac
      421 acccagaagg tccgcttctt ggccacgttt agagcgccaa cctccccccg tcgcgggcga
      481 gggacgtggg aaactacccc cacgccagcc cgcacaaacg ccgcatttga caagcttccc
      541 gattggtatg acaatgttgt ggggtgagtg agcgaggtgg gcgcaccagg ctgagggacc
      601 gggccgcgga acagctcctg gacatggtcg tctccagcgg gttgagacct gggcagcggc
      661 ttccgcccga gcgcgagctc gtggggagat tgggggtcag ccggacggtg gtgcgcgagg
      721 ccctgaacct gctggaggcg cggggtctca taagcatcga gcacggcaga ggagccgtgg
      781 taagcggggg gagcacgcgg gccgtgcggg acacgttggg gttattactg cgtgtcaggc
      841 cgaaggctct gtgggagttg ctggagatac gcgagatcct ggaggtcgag atctccggtc
      901 tcgccgccga gagggcccag gagggcgacg tgcgggagat gcgcggccag ttggacagga
      961 tggggtcgct catcgatgcg ccggagggat acgtggacgc ggacgtcgag ttccacgcgc
     1021 tgctcgcgcg cagcacgggt aatgaggtgc tcctgacgat gcttgatccc atcgtcgagc
     1081 tgctccgggc cagccgcgag gtgagcgcct cgcggcccgg cagcgcccgg cgcgctctgc
     1141 gggagcacga ggagatcctg cattgcgtcg aggccggaga tgccggagga gcgcgggaga
     1201 ggatgcgcgt ccacctcgcg aacacagcaa gggacatcga agcagcactc ggcgaagaca
     1261 tgagggagat ggatggtgta tgaaggctag agaactgctc acggatctgg acctgccagc
     1321 ccgggacctg gcggagctgc ccgactcgcc caggcgcttc cctgacggcg cccagtaccg
     1381 cgtcgagatc cccagcgtcg agggaccgcg cgtgctggag gccgtgctcg atgaggcgag
     1441 gaagcgcggg gtgcagcttc acagggtgtc gcaggggtcc ggaatcttgc tcctgaccga
     1501 tgccgagatc ctcgagatgt gcgcgatggc gcggggggct ggcctcgagc tctcgctctt
     1561 cgtcggtccg cgcgcctcgt gggagaccgg agctgccgtc gtgtccggcg cggggaaggt
     1621 gctcggggcg cagcaccggg gccaggacca gctcgcctac gccctcgaag acgtgctgcg
     1681 cggggtatct ctcgggttgc gcggcgtgct cgtcgccgac gcggggctgt tgtgggtgct
     1741 cagggacctc aaggccaaag gcgagctgcc ggaggacctg gtcgtcaagg tctcggtgca
     1801 gctctcggcg gccaacgccg ccgccctcaa agcaatggaa gacctcgggg ctgacacata
     1861 caacgtgccc acggatctga gcctcgccca gctcgccgcg atgcgggccg tcgccgacct
     1921 cccgctagac gtctacgtcg aggttcccga cgacttcggc ggcttcgtca ggcactacga
     1981 ggtgcccgac ctcgtgcggg tggcctcccc cgtattcgtg aaggcgggct tgaagaacgc
     2041 gcccaacatc tacccatcag gtggacatct ggaggcgaca gcggtggcgc tcggcagaga
     2101 gagggtgcgg cgggcggaga tcgctctctc gatgctcgag cgctactacc ccgaggccaa
     2161 gacgaccgag cggggcgcga gcttccccgg catcccggcg gcaaagagcg aggtgaacgc
     2221 atgaagatca cggacgtaga gacctttgtt gtcggcaccg actggcgcaa cctgaccatc
     2281 gtgaggctcc acaccgacga gggcctcacg ggcctcggcg aggcgcggat ggtcaaccac
     2341 accgacgcgc tggtcgggta cctggccgag gccggaccgc ggcacgtcgt cggccacgac
     2401 ccgttccgta tagaggagct ggtcaagaag ctgggccgcg acgacttctc gcgcgcggga
     2461 gaagtgctga gctcgggcat ggccgtcttc gagacggcct gctgggacat aatcggcaag
     2521 gccctggacc aaccggtata caacctcctc ggcgggccgg tgcgcgagaa ggtcaaagca
     2581 tacgccaacg gctggtaccg cgtcgagcgc accccggagg agttccacgc cgccgcccgg
     2641 aaggtagtag aacggggtta cagagccctg aagctggacc ccttcgggcc cggacactac
     2701 gagctcgagc gggacgagaa gctcagggcg gtcgcgcttg tcgaagcggt gcgggacgcc
     2761 ataggcccgg agccggagat catgctcgag atgcacggcc gcttctcgcc tgctaccgcc
     2821 gtcgagatgg cgaagatgat ggagcctttc ggcctcgggt gggtcgagga gccggtgccg
     2881 ccggtgaacc tgaaggcgct gaaaaaagcg gccgagggta tgcccggggg catcccggtc
     2941 gcgacgggcg aacggatcca ccagcggctc gagtaccgcg aggtcttcga gttgcaggcc
     3001 gcagacgtca tacagccgga cataagccac atcggcggcc tgctcgagac gaagaagctc
     3061 gccgcgtggg ccgacgccta ctacgtgacg ctcgcgcctc acaacgtggg gggccagatc
     3121 aacaccgccg ccgccctcca cctggccgcc tgcacgacta acttcaagat ccaggagtac
     3181 ttcaacgact tcgccgatcc ctgggtccgc gagaccgcgc cgggtctgcc cgaagtcgtg
     3241 gacggctact tcgccctccc acagggaccc ggcctcggcg tcgaactcga cgaggaggtc
     3301 atcgcggctc acccgaagaa gaacgtccac ttcaacctgt tcgccgaggg ctgggagaag
     3361 cgcgagacta cccggaagtc ggaccgatga tagatctctc gggcaagagg gcgctcgtca
     3421 cgggcgcggg gatgggcatc gggcagggca tcgccataga gctggcccgc cagggcgccg
     3481 acgtcgccgt ccactacgcc cacagcgaga aggacgccat cgagacagcc tcggagatcg
     3541 agcgcctcgg cgtggggcac gcgctggtgc agggcgacct gagcagggtc gtcgagtgcg
     3601 agcgggtagt ggatgaggcg gcgaactcat taggcggtct cgacgtcctg gtgaacaacg
     3661 caggcgtcac ccgcgcgctc gatttcctgg acaccgacga agctacctac gacgagatgt
     3721 tcgacctgaa catgaagggc tacttcttct gcgcccgcag agccgtgcct cttatgctcg
     3781 aggccggtgg gggttcgatc atcaacatca cctccgtcca cggctacgcc gggttccctc
     3841 gccacgccgc ctacgcagcc acgaagggtg cgatcaatgc cttcacccgc gagctggccg
     3901 tcgagctcgc agatcgcggg gtgcgggtca acgctatcgg accaggcctg atcgaagtcc
     3961 cccgctactt cgataccccc ggctattcca ccgagttcgg taacaccctg gtcccctcgg
     4021 gcaggatagg ttacccgaag gacgtcgccc cgaccgcagc tttcctcgcc tccgacgcct
     4081 ccgacttcgt taccggacag gtgatctacg tcgacggcgg taccacagcc cggatgggcc
     4141 tgtggtggga gcaggtcgag aggccacagt agcacgggcg ctagcttgac atgaacagaa
     4201 attggtatga caatgcttca cgggaaggcg ttcgaggaca gttgaggaga caggggtaaa
     4261 cggatgacgg aaaacggcac gggtagctac aggctgggac ggcgccagtt cctggcggcg
     4321 agcgctctgg cttcggcggg gctgctattg agcgggtgca ggccggaggc gcagcaggcg
     4381 agccagggtg gcggcgggtc cggtggctct ggcggcaact tccctgacac gcctgagtgg
     4441 aacttcgtgt tcgtcaacca cgtgacgacg aacccgttct tcgtgccgac gcagtacggc
     4501 atcgaggacg cgcaggcgct cctggggacg acttcgcagt ggacggggtc tgagacctct
     4561 gtggtcaggg agatggttga tgcgatgaac tcggccatct cgggtggggc caacggcatc
     4621 gcggtctcgg tggtggaccc cgaggctttc aacgacccga tccagaaggc tctcgaccag
     4681 ggcatcccgg tcatcgccta caacgccaac ggcgaggggc cggggacgaa ccctgcgctg
     4741 gcgtacgtgg ggcaggatct ctaccagtcc ggggtggaga tgggcaacag gatcgtcgac
     4801 ctcgtcgggg aagggcttgt ggcgctcttc atcgcgacgc cgggccagct caacatccag
     4861 cctcgcatag acggcgccaa gcagtccata gaggactccg gggcgaacat cgaggtccag
     4921 caggtcacga caggagccga gctgccggag gagaggtcga ggatcgaggc ctggtacaac
     4981 ggccacaagg acgtggccgg gatgttcgcc gttgacgcgg ggagcaccca gggtgttgcc
     5041 caggtgatgg agcagttcgg attgcacgac aagggagtca aggccggcgg gtacgacctc
     5101 ctgccggaga tcctgaagct catgaaggcc gggcacatag acttcaccat cgaccagcag
     5161 ccttatttgc agggcttctt cccggtcatg caga
//
LOCUS       DASTUV010000168         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_652124, whole genome shotgun sequence.
ACCESSION   DASTUV010000168 DASTUV010000000
VERSION     DASTUV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652124"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1402
                     /locus_tag="VFT03_03500"
     CDS             227..1402
                     /locus_tag="VFT03_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEU4847237.1"
                     /translation="MPTGREAGDSIPRDQDTLEGRTSTEVREEAKSVEFTYRGIFQKT
                     LAAALCKHVVLAARERDWLGSTIQRYSDSPERNGVPAKQFAVIAPDEITLQEHMAKYE
                     PNELDVITVLDDTMCDGIESWAWYGRQPINVRLRRGGTLLVISNRPPEELLKVIPQKP
                     WDWTLAILPGTPSMGGLWVYRDDGTDMRVLGAIASARPDIFDFDSIESVVGGNRGLGE
                     KYLTALKNGHDEVQVHEVKAGEGKEDDYEPVKLPGWHEMRDAIIVEAVKPGERNPHYK
                     TSSDRTMRPVVNFDTCIKCRQCWIDCPDECFEISPEALHPVNYEYCTGCGICANVCPV
                     EDCIVMVNDLEFIEEGDKDVFPMWQEDKEAYHEWRNEKIERGRIAHENFFNADTWAK"
     gene            1414..>2082
                     /locus_tag="VFT03_03505"
     CDS             1414..>2082
                     /locus_tag="VFT03_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEU4847238.1"
                     /translation="MTTQETRREVKQVEKLMTGTEAVAQAVKLADVEVTSAYPIRPYD
                     GVMQAVAKLIADGELECEYIVAEGEHSQFEICKHASSVGARVFVGSSGVGWMYAMESN
                     AVTPSLRLPVVAMIGNRALDDPGAFGVEHNDALCVRDLGWQLCWVENAQECFDATLMA
                     YRVGEDPRVSLPVGLGVDGAFITHSQALLQIPDQESVNAFLPPYDLGNRVLHPDNPIS
                     IAPQA"
BASE COUNT          431 a          623 c          700 g          328 t
ORIGIN      
        1 actccccgca ataaagcccg aaaacccgcc acagagacat acttcgtgct tgccccggga
       61 atatagagaa aatgctacaa tccacgccag gtgggattga cctgaccact cgccgctgat
      121 accatgcgcg gcaactggta aagtggtcag gccagagtct ctggcgaccg aagctggggg
      181 cttgggagat cgctcttggg gtgggtcaga gagggaagga gagggaatgc cgactggcag
      241 agaagctggg gattcgattc ctagggatca agacaccctt gaggggcgca cttcgacgga
      301 agtaagagaa gaggcgaaga gcgtcgagtt tacctatcgg gggatctttc agaagacgct
      361 ggcagcggcc ctgtgcaagc acgtggtcct ggcggcccgc gagcgggact ggctggggtc
      421 gacgatccag cgctacagcg actcgccgga gcgcaatggc gttccggcca agcaattcgc
      481 ggtcatcgcc ccggatgaga taacgctcca ggagcacatg gccaagtacg agcccaacga
      541 gctggatgtg atcacggtcc tcgacgacac gatgtgcgac gggatcgagt cctgggcctg
      601 gtacgggcgg cagccgatca acgtccgtct gaggcggggc gggactttgt tggtgatctc
      661 gaacaggccg cccgaggagc tcctcaaggt tatcccgcag aagccctggg actggacgct
      721 ggccatccta ccggggacgc cgagcatggg cgggctctgg gtctaccgcg acgacggcac
      781 ggacatgcgg gtgctcggcg ctatcgcgag cgcgcgccct gacatcttcg acttcgactc
      841 catagagtcc gtggtagggg gcaaccgggg actcggcgag aagtacctga ccgcactaaa
      901 gaacggccac gacgaggtcc aggtccacga ggtcaaggcg ggcgagggca aagaggacga
      961 ctacgagccg gtcaagctgc ccgggtggca cgagatgcgc gacgccatca tcgtcgaggc
     1021 cgtaaagccg ggcgagcgca acccgcacta caagacctcc tcggatcgta ccatgcggcc
     1081 tgtggtcaac ttcgacacct gcatcaagtg taggcagtgc tggatcgact gccccgacga
     1141 gtgcttcgag atcagccccg aggcgctgca ccccgtcaac tacgaatact gcaccggttg
     1201 cggcatctgc gcgaacgtct gcccggtcga ggactgcatc gtgatggtca acgacctcga
     1261 gttcatcgag gagggcgaca aggacgtctt ccccatgtgg caggaggaca aggaagccta
     1321 ccacgagtgg cgtaacgaga agatagagcg cgggcggata gcccacgaga acttcttcaa
     1381 cgccgacacc tgggcgaagt gagaggaggg gagatgacca ctcaagagac caggcgcgag
     1441 gtaaagcaag tagagaagct gatgaccggc acggaggccg tcgcccaggc cgtgaagctg
     1501 gcggacgtcg aggtgacgtc ggcttacccg atacggccct acgacggcgt tatgcaggcg
     1561 gtcgccaagc tcatcgccga cggcgagctc gagtgcgagt acatcgtcgc cgagggcgag
     1621 cactcccagt tcgagatctg caagcacgcc tcctccgtcg gggcccgcgt gttcgtgggc
     1681 tcatccggtg tgggctggat gtacgcgatg gagtccaacg cggtcacgcc ctcgttgagg
     1741 ctgccggtcg tggctatgat cggcaaccgg gcactcgacg atcctggagc gttcggcgtc
     1801 gagcacaacg acgctctgtg cgtgcgagat ctcggctggc agctctgctg ggtggagaac
     1861 gcccaggagt gcttcgacgc tacgctcatg gcctaccgcg tcggcgagga ccccagggtc
     1921 tctttacctg tcggccttgg tgtggacggc gcgttcatca cgcactcgca ggcgctgctc
     1981 cagatcccgg accaggagag cgtcaacgcc ttcctgccgc cctacgacct ggggaaccgg
     2041 gtgctgcacc cggacaaccc gatcagcata gccccccagg cc
//
LOCUS       DASTUV010000169         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_693994, whole genome shotgun sequence.
ACCESSION   DASTUV010000169 DASTUV010000000
VERSION     DASTUV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693994"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFT03_03510"
     CDS             complement(<1..807)
                     /locus_tag="VFT03_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847239.1"
                     /translation="MGPHRLRQASSRLPEERLGLPGEPDRRRGVQHRLAWRPVGSGFL
                     GTGGFQALAEYVGRRILQIIPVLLGVSIVVFFMVRAIPGDPAQLLLGQQATQAQVEQV
                     RENMGLDKPVIVQYGIFLADAVRGDFGDSIVTGRPVTTELLARLPATLELTAFAMLVA
                     ILVGVPVGVISAVKQYSLLDKFTSVLALTGISMPIFWLAMILIVIFFVNLGWLPFPGR
                     LSTGYAVTSISGFVLVDSLLTLNFAAFWDGLKHLILPAIALGTIPMAVIMR"
     gene            complement(690..2387)
                     /locus_tag="VFT03_03515"
     CDS             complement(690..2387)
                     /locus_tag="VFT03_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4847240.1"
                     /translation="MARKSIQISRRDFLRLSGAGLAGATLLGVAGCGGGGTIQGGGGG
                     GGGGAGTNTFVFGRGADSVSLDPVNTTDGESLRVTRQIFDGLLDFAPESTDVIPALAT
                     EIPEPEDGGRAYTFKLRDGVKFHDGAEFNAEAVKFNFDRWRDSKNPYHTGGGGQSENF
                     AYYVGQFGGFDDDSIITGVDVVDEYTVRINLKEPQGPFLKNVAMSPFGMASPKAIKED
                     VESFWQNPIGTGPFKFVEWNRGSTVELEANEDWWGSEVPESEGGGGPIVKKVTIRSIP
                     DNTSRVAALTGGELSAADGLTPDDVPTVKQSGLKVEFRPPLTIGYLAMNVQKEPFNDV
                     NVRRAFNMAIDMDAIVKAFFGDTGEVATTYMPPTVPFFNESIEHYPFDPDKARQMLQD
                     AGVDNLETDLWYMPIPRPYMPDGKGIAQAMQQDLKEVGVNAKLVTYEWGTYLERTGSG
                     DHSTALLGWTGDNGDPDNFLNVLLGSASATETDALNIAYYKNPEVDKLLLKGQSSIEE
                     SEREPAYMKAQELLVQDAPWVPIAYAKPPLGFQKNVSGYQASPTGGEAFNTVSLGGQ"
     gene            complement(2468..>2834)
                     /locus_tag="VFT03_03520"
     CDS             complement(2468..>2834)
                     /locus_tag="VFT03_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEU4847241.1"
                     /translation="DAQGLAHPRRMGLASHVGLFVDVPTVGCAKSVLVGAFEEPEVEK
                     GSATDLVHRGEVVGRAVRTRERVSPVYVSVGNRIDLDSSVELVLAGSTRYRLPEPTRQ
                     AHNAANRLRRGEDPGGSLF"
BASE COUNT          476 a          945 c          884 g          529 t
ORIGIN      
        1 gcgcatgata accgccatag gtatcgtccc cagggctatg gccggcagga tgaggtgctt
       61 gagaccgtcc cagaacgcag cgaagttgag cgtgagcaac gagtcaacga gcacgaaccc
      121 ggatatggag gtgacggcgt aaccggtaga cagacgtccc gggaaaggca gccaccccag
      181 gttcacgaag aagatgacga tcaggatcat cgcgagccag aagatgggca tgcttatccc
      241 cgtgagcgcg aggaccgaag tgaacttgtc cagcaacgag tactgcttga ccgccgagat
      301 cacgcccacg ggcacaccga ccaggatcgc gacgagcatg gcgaacgctg tgagctcgag
      361 cgtggccggg aggcgcgcga ggagctcagt cgtgaccggc cgccccgtga ctatggagtc
      421 cccaaagtct cccctcacgg cgtcggcgag gaagatcccg tactgtacga tgacgggctt
      481 gtcgagcccc atgttctccc ttacttgctc gacctgcgcc tgtgtcgcct gttgcccgag
      541 gagcagctgt gccgggtcgc ccgggatggc gcgcaccatg aagaacacca cgatggacac
      601 cccgagcaac accgggatga tctgcaagat tctgcgcccg acgtactccg ccagagcctg
      661 gaaacctcct gtgcctagaa acccgctccc tactggccgc caagcgagac ggtgttgaac
      721 gcctcgccgc ctgtcgggct cgcctggtag cccgagacgt tcttctggaa gcctagagga
      781 ggcttggcgt aggcgatggg gacccacggg gcgtcctgaa ccaggagctc ctgagccttc
      841 atatacgccg gctcgcgctc gctctcctcg atgctggact ggcccttcag gagcagcttg
      901 tccacctcgg ggttcttgta gtaggcgatg ttgagcgcgt cggtctcggt cgcgctcgcg
      961 ctgccaagga gcacgttgag gaagttgtcg gggtcgccgt tgtccccggt ccacccgagc
     1021 agtgccgtcg agtggtcgcc ggagccggtc ctctcgaggt aggtgcccca ctcgtaagta
     1081 acgagcttgg cgttcacgcc gacctccttg agatcctgct gcatcgcctg ggctatgccc
     1141 ttgccgtccg gcatgtaggg cctcgggatc ggcatgtacc ataggtcggt ctccaggtta
     1201 tcgacacccg cgtcctggag catctgcctc gccttgtccg ggtcgaaggg gtagtgctct
     1261 atgctctcgt tgaagaacgg cacggtaggc ggcatgtagg tcgtcgctac ctccccggtg
     1321 tctccgaaga aggccttgac gatcgcgtcc atgtctatgg ccatgttgaa cgcccgccgc
     1381 acgttgacgt cgttgaaggg ctccttctgg acgttcatcg ccaggtaccc gatcgtgagc
     1441 ggcggccgga actccacctt taggcccgac tgcttgacgg tagggacgtc gtcgggggtg
     1501 aggccgtcgg ccgcggagag ctcgccgcca gtgagcgcag ccaccctcga ggtgttgtcc
     1561 gggatggagc ggatcgtgac cttcttgact ataggaccac ccccgccctc gctctcgggc
     1621 acctcagagc cccaccagtc ctcgttggcc tcgagctcga cggtggagcc cctgttccac
     1681 tccacgaact tgaacgggcc ggtgccgata ggattctgcc agaagctctc tacgtcctcc
     1741 tttatcgcct tgggcgaggc catcccgaac gggctcatgg cgacgttctt gaggaatggt
     1801 ccttgcggct ctttgaggtt gatccttacc gtgtactcgt ccacgacgtc gacgcccgtt
     1861 attatggagt cgtcgtcgaa gcctccgaac tggccaacgt agtaggcgaa gttctcgctc
     1921 tggcctccgc cgcccgtgtg gtatgggttc ttgctgtcgc gccagcggtc gaagttgaac
     1981 ttgacagcct cggcgttgaa ctcggcgccg tcgtggaact tgacgccgtc cctgagcttg
     2041 aaggtgtagg cccgcccgcc gtcctcgggc tcggggatct cggtcgcgag cgcgggtatt
     2101 acgtcggtcg actcgggcgc gaagtcgagc aggccgtcga agatctggcg cgtcacccgc
     2161 aacgactcgc cgtccgtggt gttgacaggg tcgagcgata cagagtcagc cccgcgcccg
     2221 aagacgaagg tgttagtccc cgcgcctcca ccgcctccac caccgccgcc ctggatggtc
     2281 ccgcccccgc cgcaacccgc aacgccgagc agcgtcgcgc cggccaggcc cgcgccgctc
     2341 aacctgagga agtcacgacg gcttatctgg atgctttttc ttgccaaagc atgctccctt
     2401 ctgaccccga tcggatgaaa tatccgcgtt accacgtacg gtatcatttg ctcttccgcg
     2461 ccgccaccta gaagagcgag ccccccgggt cctcgcccct tcgcagcctg ttggccgcgt
     2521 tgtgggcctg gcgcgtgggc tccggcaagc ggtacctggt tgaaccggcg agcaccagct
     2581 cgaccgaact gtccaggtct atcctgtttc ccacggagac gtagaccggc gagacccgct
     2641 cccgcgttcg cactgcccta cccacaacct ctccgcggtg caccaggtcc gtggcgctgc
     2701 ccttctcgac ctcgggctcc tcgaaggcgc cgacgagcac gctcttcgcg caccccaccg
     2761 tgggaacgtc gacgaacagc ccgacgtgcg aagcgagccc catccgccgc gggtgggcga
     2821 gcccctgggc gtcc
//
LOCUS       DASTUV010000170         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1188608, whole genome shotgun sequence.
ACCESSION   DASTUV010000170 DASTUV010000000
VERSION     DASTUV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188608"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..905
                     /gene="fabG"
                     /locus_tag="VFT03_03525"
     CDS             159..905
                     /gene="fabG"
                     /locus_tag="VFT03_03525"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU4847242.1"
                     /translation="MIEAGRVAVVTGGTRGIGRTIAMRLAAEGANVALSYRSNDEAAE
                     ETADSVRAEGVKCAVFRGDVASAGDVQALFKGVSDEFGRLDILVNNAGITRDNLMMRM
                     KEDEFDEVLRTNLGGTYLCTRAALRPMIRARWGRIVNVSSVVGLVGNAGQANYAASKA
                     GIIGFTKSVAREVAQRGITANTVAPGYVETELTGSLPEEVKDQIRNQVPMGRFGEAVE
                     VAEVVAFLAGEGAGYVTGQTIAVDGGMTMQ"
     gene            984..1229
                     /gene="acpP"
                     /locus_tag="VFT03_03530"
     CDS             984..1229
                     /gene="acpP"
                     /locus_tag="VFT03_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4847243.1"
                     /translation="MDREEILGKIQEITADRLGVDESDVTPDASFREDLEADSLDLVE
                     LIMELEEQFGMEIPDEEAEKITTVEEAVDYVQEHQTA"
     gene            1226..2479
                     /gene="fabF"
                     /locus_tag="VFT03_03535"
     CDS             1226..2479
                     /gene="fabF"
                     /locus_tag="VFT03_03535"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU4847244.1"
                     /translation="MTEEGANSNGYALITGAGAVTPLGAGVENFWDAALHSKSGIGEI
                     TLFDSSPFPSQIAGECSDFDPTDFMSKKAARRMDRFAQLGIAAGLEAAESAGIADVVR
                     ERPERVAIVMGSGIGGLSTWEREYRVLSERGPGRVSPFLITMMVPNMAAGQLAIMLGA
                     TGASQCPVLACATGAEAIAEGLELIRRGDADVVIAGSSEAPITPLSMAGFCAIRAVSR
                     RNDDPHGASRPFDKDRDGFVMSEGAGALILEGPEHAERRGADAIAAVSGYGRTTDAYH
                     VTAPDEEGRGVERAMRLALEDSGLTPEDVGHVNAHATATPTGDGPETKAISRVMPHAL
                     VSGTKSMTGHSFGAVGATEGIMCALAIKNEVVPPTINLENLAEDCEELNYVGAESREA
                     PNLKAAISNSMGFGGHNVVVAFSKV"
     gene            2532..3152
                     /locus_tag="VFT03_03540"
     CDS             2532..3152
                     /locus_tag="VFT03_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEU4847245.1"
                     /translation="MKQRSLTHPSVADPYDSNGRLEFLGDSVLNSRVAELVFHGYPEL
                     LEGDLTRIRAHLVRKTFLATVGRAEGLEEIIESSVINDSVIADTVEAIIGAAYLIDRD
                     LVLRTIDEIFNPSEVDTDELRDWKTLLQETLQADGLRPTYRVISKQGPAHRPSFISGV
                     SADGREVATGEGSSIKESEQAAARAALEILGIRPVPRSAVEVHVGA"
     gene            3324..>3943
                     /locus_tag="VFT03_03545"
     CDS             3324..>3943
                     /locus_tag="VFT03_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4847246.1"
                     /translation="MLSGIYIKGFKTFARPVRMPLEGGVTAIVGPNGSGKSNITDAVL
                     FALGEQSAVVLRAGGMSELIFSGSETLPPAGAAEVTLVLDNALGEISLPYREVSLTRR
                     ISRAGDTEYRINGTRARLQDVRAVAGEVGIGRHSILRQGAVDAIVAGGAAACRLALEE
                     AAGLGVYRRRRLSASRRLEKAASQLEQTRQLESELESQLRRMEREAA"
BASE COUNT          749 a         1193 c         1388 g          613 t
ORIGIN      
        1 cgccggcagg tggtgcccgg cgacgttctg aggatggagg tggagataga tcgcctcaag
       61 gggcgggtcg ggcggggcac gggccgggca gcggtcggcg gcgagcgggt ctgcgaggcc
      121 cagctcatgt tcgctttcgc agagagggga gaggtcgagt gatcgaggcc ggacgtgtgg
      181 ccgtcgttac cgggggcacg cgcggcatag gcagaacgat agccatgcgg ctcgcggcgg
      241 agggcgccaa cgtggccctc tcctaccgct cgaacgacga ggctgccgag gagacggccg
      301 actcggtgcg cgcggagggc gtgaagtgtg cggtcttcag gggggatgtg gcttcggccg
      361 gagacgtgca ggccctcttc aagggcgtga gcgatgaatt cgggcggttg gacatactcg
      421 tcaacaacgc cgggataacc agggacaacc tgatgatgcg gatgaaagag gacgagttcg
      481 acgaggtgct caggaccaac ctcgggggca cctatctttg cacgcgtgct gcgttgaggc
      541 cgatgatcag ggcgcggtgg ggcaggatcg tcaacgtgag ctccgtcgtc gggctcgtcg
      601 ggaacgccgg gcaggccaac tatgcggctt cgaaggccgg gatcatcggg ttcaccaaga
      661 gcgtcgcgag ggaggtcgcg cagcgcggga tcacggccaa cactgtggcc ccgggatacg
      721 tggagacgga gctgaccggg agcttgcccg aggaggtgaa ggaccagatc cggaaccagg
      781 tcccgatggg acgcttcggc gaagccgtgg aggtcgcaga ggtagtagct tttttggcgg
      841 gcgaaggagc cggttacgtc acgggccaga ccatagccgt cgacggcggc atgactatgc
      901 agtaaggcgt ttagcgggta cactacgcgc tgtgcccact tggggtaaat gagcttgtcg
      961 taatttgtgg agaggagagg ggcttggatc gcgaagagat actgggcaag atccaggaga
     1021 taaccgccga ccggctgggt gtggacgaga gcgacgtgac tcctgacgcg tcgtttcggg
     1081 aggacctcga ggcggactcc ctagatctgg tcgagctcat catggagctc gaagagcagt
     1141 tcgggatgga gatacccgac gaagaggccg agaagatcac cacggtcgag gaagcagtgg
     1201 attacgtgca ggagcaccag acggcgtgac cgaggagggg gcgaactcca acggctacgc
     1261 gctgatcacc ggcgcagggg ccgtgacgcc tctgggcgcc ggggtcgaga acttctggga
     1321 tgcggcgctg cactccaaga gcgggatcgg agagatcacg ctcttcgact cgtcgccttt
     1381 cccctcccag attgcgggtg agtgctcgga cttcgacccc accgacttca tgagcaagaa
     1441 ggcggccagg aggatggacc gcttcgccca gctcggcatc gccgcaggac tggaggccgc
     1501 agagagcgcc gggatagcgg acgtcgtgcg cgagagaccc gagcgggtcg cgatagtgat
     1561 gggcagcggc atcggcgggc tctcgacctg ggagcgcgag taccgggtct taagcgagcg
     1621 gggaccgggg agggtgagcc cgttcctgat cacgatgatg gtcccgaaca tggcagcggg
     1681 ccagctcgcg atcatgctcg gcgccacggg cgcgagccag tgccccgttc tggcctgcgc
     1741 cacaggagcc gaggcgatag ccgaagggct tgagctgatc cggcgcggag acgccgacgt
     1801 cgtcatagca ggctcctcgg aagccccgat cacacccctg agcatggccg gcttctgcgc
     1861 gattcgggcc gtcagcaggc gcaacgacga cccgcacggc gcgagccgcc ccttcgacaa
     1921 ggaccgcgac ggcttcgtga tgagcgaggg cgcgggcgcc ctcatcctcg agggcccgga
     1981 gcacgccgag cggcgtggcg ccgacgcgat agccgccgtc tccggttacg gtcgcacgac
     2041 cgacgcatac catgtgaccg ctcccgacga ggagggccgc ggcgtcgagc gcgcgatgcg
     2101 tctggccctc gaagactccg gcctcacgcc cgaagacgtc gggcacgtca acgcccacgc
     2161 cacggcgacc cccaccggcg acggccccga gacgaaggcg atctccaggg tcatgcccca
     2221 cgcgctagtc tccggcacca agtccatgac gggccactcc ttcggcgcgg tcggcgctac
     2281 agaggggatt atgtgcgccc tcgccatcaa gaacgaggtg gtcccgccga ctatcaacct
     2341 ggagaacctc gccgaggact gcgaggaact gaactatgtg ggggcggaat ccagagaggc
     2401 cccgaacctc aaagcggcca tctcgaactc gatgggcttc ggcgggcaca acgtcgtggt
     2461 agcattctcg aaggtgtaga atagagaacg aagtgtctat aaaggaccta ataactgttc
     2521 taccggagcc tttgaagcag cgctcgctca cccacccgag cgtagccgac ccctacgact
     2581 ccaacggccg gctggagttc ctcggggact cggtcctcaa cagcagggtg gccgagctcg
     2641 tcttccacgg ctaccccgag cttctggagg gggatctcac gcgcatccgg gcccacctgg
     2701 tccgcaagac attcctggcc accgtcggcc gggcggaagg actcgaggag atcatcgaga
     2761 gctccgtcat aaacgactcg gttatcgccg acacggtcga agcgataatc ggggctgcct
     2821 acctaataga tagagacctg gttctccgca caatagacga gatcttcaac ccctccgaag
     2881 tggacacaga cgagctgcgc gattggaaga ccctgcttca ggagacgctc caggccgacg
     2941 gcctgaggcc gacctatcgc gtgatctcga agcaggggcc ggcgcaccgg cccagcttta
     3001 tctccggggt ctccgctgat ggccgcgagg tggccaccgg cgagggcagc tccatcaaag
     3061 agagcgaaca ggccgccgca cgcgcagcgc tagagatcct gggcatccgc cccgtcccac
     3121 gctccgccgt cgaagtacac gtcggagcgt aagtaagcac gctacgccct tttagggctc
     3181 cgctctcagc acgctcaggc ttcgccaggc ggcttctcag cttctctctg caccaagaga
     3241 agctgaaatg ctgacaagcc gaccgtaggg aggcgtgctt acttgcgtgc tttttgcacg
     3301 caagttacgt agaatgagtt tccgtgctct cggggatcta tatcaagggc ttcaagacat
     3361 ttgccaggcc ggtcaggatg cctttggagg gcggcgttac agccatagtc gggccgaacg
     3421 gctcgggcaa gagcaatatc acagacgccg tgctcttcgc gctcggggag cagagtgcgg
     3481 tcgttttgcg ggcaggtggg atgagcgagt tgatcttctc gggctccgag acgctgccgc
     3541 cagccggagc tgccgaggtt acgctcgtcc tggacaatgc cttgggcgag atctcactgc
     3601 cgtaccggga ggtctcgctc acccgtcgca tctcgcgcgc cggcgacacg gagtacagga
     3661 tcaacggcac ccgcgcgcgc ctgcaggacg tccgagcggt cgcaggtgag gtcgggatcg
     3721 gacgccacag tatcctgcgg cagggggccg ttgatgccat agtcgcgggt ggcgctgcag
     3781 cctgccgcct cgccctcgag gaggcagcgg gcctgggggt ttaccgccga cgtcgcctct
     3841 ccgcgagccg ccgcctggag aaggccgcat cccagctgga gcagacccgc cagctagagt
     3901 cagagctcga gagccagttg cggaggatgg agagggaggc cgc
//
LOCUS       DASTUV010000171         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_401521, whole genome shotgun sequence.
ACCESSION   DASTUV010000171 DASTUV010000000
VERSION     DASTUV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401521"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1020)
                     /gene="galT"
                     /locus_tag="VFT03_03550"
     CDS             complement(31..1020)
                     /gene="galT"
                     /locus_tag="VFT03_03550"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU4847247.1"
                     /translation="MNQLRWDPTLREWVAYATHRQDRTFLPPAEYCPLDPTKPGGFPT
                     EVPRESYDIVIFENRWPSFAPDAPQPEEEGSPLTPTAPGRGICEVVLYSEDHYATLAG
                     MSGGRIRNLIEVWADRYTELGSVDFVDYVFIFENKGEAIGVTLHHPHGQIYAYPFIPP
                     RPKKELEAAVEYRSENEHRCLHCDLLSQEHEDGRRLVAKGEHFTAFIPFYAHFPYEAH
                     VYARRCVPSIAGLSAEERRDLARVLKRLLVGYDRLFGFSLPYMMVMHQAPTDGGDYEG
                     VAHFHIEFYPPNRTADKLKYLAGSETGAGAYVMDALPEQTAERLREAVGSRAG"
     gene            complement(1017..1175)
                     /locus_tag="VFT03_03555"
     CDS             complement(1017..1175)
                     /locus_tag="VFT03_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847248.1"
                     /translation="MDRPENNPADVHAAGAEDVPRELEMVKERETKEDGRYIIFYSFG
                     EAREDGDC"
     gene            complement(1543..2403)
                     /locus_tag="VFT03_03560"
     CDS             complement(1543..2403)
                     /locus_tag="VFT03_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4847249.1"
                     /translation="MVAGGEGPKPRVLLTGATGYIAGQLLPAFRERYDLVLLDVREED
                     RSGNRVEGVEVVDLLGDDRARLERLFSDAEVVVHTGHLKPGGTDPQAQYDGERRNVDM
                     MQRVYQLALDCGVRRVVAASTNQAAKWYERPYFAGLKDRVSPEDYPRPDNFYGWAKAA
                     YEPLGFLYACGSLGRKLEVIQLRIVVPRQIDADDFTDEPPERYVRELAGYVSERDLQQ
                     LFCKSVETPDIEDQYGVPFHIFYGVSNNARKFWSITNARKVIGYEPQDDSEVSFAADI
                     VRLLHRRKSS"
     gene            complement(2397..3383)
                     /locus_tag="VFT03_03565"
     CDS             complement(2397..3383)
                     /locus_tag="VFT03_03565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4847250.1"
                     /translation="MKYRNLGETGMRVSEISLGTWAFGSEWGEVSDDESYATLNRAID
                     LGVNFLDTADVYGDGRSEKLIGRLLRERPDDEIFVATKAGRRLDPHTAEGYDYEHLSA
                     FVERSLDNLGVEALDLLQLHCPPTGVYRRDETFEALDRLQAAGKVKHYGVSVEKVEEA
                     RMALSYPGAKTVQVIFNIFRQKPAEEFFPLAEERNIGVLARVPLASGLLSGKMSADRI
                     FSEDDHRNFNREGQAFDRGETFSGVNFETGLRAAEELKSLVPEGYTLAQLALRWILMH
                     QAVSCAIPGAKRPDQLEDNLAAAEMPALSEETMQRIREIYDRSVRPEVHHLW"
     gene            complement(3396..>3878)
                     /locus_tag="VFT03_03570"
     CDS             complement(3396..>3878)
                     /locus_tag="VFT03_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU4847251.1"
                     /translation="ANGRGNSDLKTAERFSDLDATTVLPEGVETTAMGWPVEADGLTE
                     LLVRLHVEYTQVPIYITENGRAVYDYVDPEGEVEDEERISYLDAHFRAAHDAMQQGVD
                     LRGYMVWSLLDNFEWAEGYSKRFGIVFVEYGTQRRIPKTSARWYRDVIERNGLPEGGS
                     "
BASE COUNT          635 a         1217 c         1256 g          770 t
ORIGIN      
        1 cgtaacccgc cccgccggta acgagtagct tcacccggcc ctgcttccga cggcctccct
       61 gagcctctct gcggtctgct ccggcagggc gtccatcacg taggctccgg cgcccgtctc
      121 gctgcctgcg aggtacttga gcttgtccgc ggtgcggttg ggcgggtaga actcgatgtg
      181 gaagtgcgcc acgccttcgt agtccccgcc atccgtgggg gcctggtgca tgaccatcat
      241 gtacggcagc gagaagccga agagccgatc gtagcccaca agaagccgct tgaggacgcg
      301 ggcgagatct cgcctctcct ccgcgctaag gccggctatg gaaggcacgc aacggcgggc
      361 gtagacgtgc gcctcgtagg ggaagtgcgc gtagaatggg atgaaggcgg tgaagtgctc
      421 tcccttcgcg acgagccgcc ggccgtcctc gtgctcctgg gagagtaggt cgcagtgcag
      481 gcagcgatgc tcgttctccg aacggtactc tacggccgct tcgagctctt tcttcgggcg
      541 cggtgggatg aacgggtagg cgtagatctg gccgtgcggg tggtggaggg tcacgccgat
      601 ggcctcacct ttgttctcga agataaagac gtagtccaca aagtccactg agccgagctc
      661 cgtgtagcgg tcggcccaca cttcgatgag gttgcggata cgccccccgc tcattcccgc
      721 aagagtagcg tagtgatcct ctgaatagag gaccacctcg cagatcccac gtcccggagc
      781 ggtgggcgtg agcgggcttc cctcttcctc cggctgcggg gcgtcgggcg cgaaggaggg
      841 ccacctgttc tcgaagatca cgatgtcgta ggactcgcgg ggaacctcgg tcgggaagcc
      901 gccgggtttc gtcggatcta ggggacagta ctcggcaggt gggaggaagg tgcggtcctg
      961 gcggtgggtg gcgtaggcaa cccactcgcg cagcgtcgga tcccaacgga gctggttcaa
     1021 cagtcaccat cctctcgcgc ttcgccgaag gagtagaaga tgatgtagcg gccgtcctct
     1081 ttcgtctcac gctccttgac catctcgagc tctcggggga cgtcctccgc cccggcggcg
     1141 tgcacatcag caggattgtt ctcgggacgg tccaacgcat tcacccttta gcccgctgca
     1201 ggagagatgc cctcgatgac gtacctctgg gcgaggatag tgaggatttc cttctcctgt
     1261 tctctttctc cctaaaatac tggttaccca acggtagtgc cgtgaaccct agcaataata
     1321 tgaacactag ttaactataa gcaacaaatg cagtgttgct tcatttggct tgggcgtata
     1381 cttgcgtcgc ggttcagcat ctcttctccg agcgaggccg cgaggcgcag cgagaggccg
     1441 ggctcgtagc cttcgacgct gacctcgaca tacacgcagg ccgcgtccac tcggggcagc
     1501 ggagctgggg acgagggtat tcggggcgag gaaggtctct catcaacttg acttccgccg
     1561 gtgcaacagc cggacgatgt ccgcggcgaa gctgacctcg gagtcgtcct ggggctcgta
     1621 gccgatcact ttgcgggcgt tggtgatgct ccagaatttg cgggcgttgt tcgagacccc
     1681 gtagaatatg tggaacggca cgccgtactg atcctctatg tcgggcgtct cgacggattt
     1741 gcagaagagc tgttgcaggt cccgctcgct gacgtagcct gcgagctcgc ggacgtagcg
     1801 ttcggggggc tcgtcggtga agtcgtcggc gtctatctgg cgcggtacga cgatgcgaag
     1861 ctgtatcacc tcgagcttcc ggcccaggct gccgcaggcg tacaggaatc ccagaggctc
     1921 gtaggcggcc ttggcccagc cgtagaaatt gtccgggcgc gggtagtctt cggggctcac
     1981 gcggtctttg agcccggcga agtaaggacg ctcgtaccac ttcgccgcct ggttagtgct
     2041 ggcagcgacc acacgccgca cgccgcagtc gagcgcgagc tggtagacgc gctgcatcat
     2101 atccacgttt cgtcgctcgc cgtcgtattg ggcttgtggg tctgtaccgc ccggcttcaa
     2161 gtgcccggtg tgaaccacga cctcggcgtc cgagaatagg cgctcgaggc gggcccggtc
     2221 gtcacccagc aagtccacaa cctccacccc ttcgacccgg ttgccggagc ggtcctcctc
     2281 ccgaacgtcg agcagcacga ggtcgtagcg ttccctgaac gccgggagca gctgacccgc
     2341 gatgtatccc gtagctccgg tcaaaaggac tcgcggcttc ggtccctctc ccccggctac
     2401 catagatggt ggacctcggg acggacgctg cggtcgtaga tctcgcgtat ccgctgcatc
     2461 gtctcctccg agagcgcggg catctcggcg gcggcgaggt tgtcctcgag ctggtcgggc
     2521 cgcttcgcgc cggggatggc gcaggagaca gcctggtgca tcaggatcca gcgcagcgcc
     2581 agttgcgcca gggtgtagcc ttcggggacg aggctcttta gctcctcggc cgccctgagg
     2641 ccggtctcga agttcacgcc cgagaaggtc tcgccgcggt cgaaggcctg tccttcacgg
     2701 ttgaagttgc gatggtcgtc ctccgaaaat atgcggtcgg cgctcatctt gcccgagagg
     2761 aggccgctgg cgagcggtac gcgggcgagt acgccgatgt tccgttcctc ggctagtggg
     2821 aagaactcct cagcgggctt ctggcggaag atgttgaaga tgacctgcac ggtcttcgcg
     2881 ccgggataag acagagccat ccgcgcttcc tcgaccttct cgacgctgac gccatagtgc
     2941 ttcaccttgc ccgccgcctg gagccggtcc agagcttcga acgtctcgtc ccgcctgtag
     3001 acgccggtcg gagggcagtg gagctggagg agatccagag cttcgacgcc caggttatcg
     3061 aggcttcgct cgacgaaggc ggagagatgc tcgtagtcgt agccttccgc ggtgtgagga
     3121 tcgaggcggc gtccagcctt cgtcgcgacg aagatctcgt cgtccggcct ttctctgagc
     3181 aacctgccga tgagcttctc gctgcggccg tcgccgtaga cgtcggcggt gtcgaggaag
     3241 ttcacgccga ggtcgatagc cctgttcaga gtggcatacg attcgtcgtc gctcacctcg
     3301 ccccactcgc ttccgaaggc ccaggtcccg aggcttatct ccgagacccg catgcccgtc
     3361 tcccccaggt ttcggtactt caagttcgtc cctttctagg agccgccttc cggtagcccg
     3421 ttacgctcga ttacgtctct gtaccagcgg gcgctcgtct tcgggatgcg ccgctgggta
     3481 ccgtattcta cgaagactat gccgaagcgt ttggagtaac cctcggccca ctcgaagttg
     3541 tccagcagcg accacaccat gtacccgcgg aggtccacgc cctgctgcat cgcgtcgtgg
     3601 gcggcgcgga agtgggcgtc gaggtaggag atgcgctcct cgtcttcgac ctcgccctcc
     3661 gggtccacgt agtcgtagac ggcccggccg ttctcggtaa tgtagatagg tacctgcgtg
     3721 tactccacgt ggaggcgcac gagaagctcg gtgaggccgt ccgcctcgac gggccagccc
     3781 atcgcggtcg tctcgacgcc ctcgggcaag acggtcgtcg cgtccaaatc cgagaaacgc
     3841 tcggcggtct tcaggtccga attcccccgg ccgtttgc
//
LOCUS       DASTUV010000172         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_610439, whole genome shotgun sequence.
ACCESSION   DASTUV010000172 DASTUV010000000
VERSION     DASTUV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610439"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..2955)
                     /locus_tag="VFT03_03575"
     CDS             complement(271..2955)
                     /locus_tag="VFT03_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4847252.1"
                     /translation="MATRMCDVCGIRPAVVTVRRVVPGEGQRVEHLCEIHAAEAGVGR
                     STFGGRSPLGGGSLFDDFFDRFFDEEPRGSPRGGGVVPRRQAEQVDITQFFNDSTTEL
                     LQRAAGQAVEWGNLDLSSEHLLYAALEDNVVRRVLEGVEADPEAIRAQLEEEADKGGR
                     TDVTPSLAPDAKRALLAAYEESQALGSSYIGPEHVLLALARDDESEAGELLGRFGLSH
                     TRLRGAVMRGVDTATGAAREPASATPTLDEYSRDLTQMAREGKLDPVIGRAEEVETTI
                     EILSRRTKNNPVLIGDPGVGKTAIVEGLAQRIVNDEVPETLVGRRVLALDLSGLVAGT
                     QYRGQFEERLKQVVDEASENPEGQILFIDELHTVVGAGAAEGSMDASNMLKPALARGE
                     LHVVGATTVDEYRKNVEKDAALERRFQPVLVGEPSVDDTIDILRGLKDRYEAHHRVKI
                     TEEAIVAAAELSDRYVTDRFLPDKAIDLMDQASARVRLRSKTKPRDTKELEEEVARLG
                     REKDQAVSAEDFGRAQELKGRVEGLRSELDGSRDGARRPVAEVTAEDIAEVVSRATGI
                     PVSQLTQEERERLMRLEEQLHERVVGQDEAVTAVAEAIRRARAGLSDPNRPIGSFLFL
                     GPTGVGKTELARTLAEALFGDEAAMVRIDMSEFQERHTVSRLVGAPPGYVGYEEAGQL
                     TESVRRRPYSVLLLDEIEKAHPDVFNILLQILDDGRLTDSQGRTVDFKQTVIIMTSNM
                     GAQRIQAHARRDESFEELKDDMMQILKNTLRPEFVNRIDEVIVFRSLGKEQIGEIARL
                     LLDRTTRRLRAQGIEVEFTEGAVGLIGEEGFDPEFGARPLRRTIQRRVDNELASMVLS
                     GSLDPGDRVVVGAAGESGGLTFEVVAGAAAAEPPGEGG"
BASE COUNT          445 a         1074 c          963 g          573 t
ORIGIN      
        1 accgccacgt cccacgagac ccctgcgtcg acccggctgc ggaggagatc gggcagttcg
       61 gaagccagct cctcggcgat cttctcgggg atgtcggggg cgggtatcag tcccagcgtc
      121 acagtgcggg ccacgagcac ctcctggcag tgcctgtcgt aacgagtact accacagacc
      181 taacactcgc gtagcggggt taaaccgagg ggcggtcgtg gtccacggtt ggcgtaatcc
      241 cctattcagc ggagtacgtg gagggagcgt tcaccctccc tcgcccgggg gctccgccgc
      301 ggccgccccc gcgacgacct cgaaggtgag cccgccggat tcaccagcag cgccgacaac
      361 caccctgtcg ccgggatcca aggagcccga gaggaccatg ctggccagct cgttgtccac
      421 ccgccgctgt atcgtacgcc tcaagggcct cgccccgaac tcggggtcga agccctcttc
      481 gcctatcagc ccgaccgccc cttcagtgaa ctcgacctct atgccttggg ccctcaggcg
      541 cctcgtcgtc cggtcgagca ggaggcgagc gatctcgcct atctgctcct tgccgaggga
      601 gcggaagacg atgacctcgt ctatgcggtt gacgaactcg ggacgcaggg tgtttttgag
      661 gatctgcatc atgtcgtcct tgagctcctc gaaggactca tcacgcctgg cgtgcgcctg
      721 tatcctctgt gcgcccatgt tggaggtcat gatgatcacc gtctgcttga agtccactgt
      781 ccggccctgc gagtccgtca ggcgtccgtc atccaggatc tgcaggagga tgttgaagac
      841 gtccgggtgg gccttctcaa tctcgtccag gagcaagact gagtagggcc tcctcctaac
      901 gctctcggtg agctggccgg cctcctcgta gcccacgtag ccgggagggg ccccaaccag
      961 gcggctcaca gtgtgccgct cctggaactc gctcatgtct atgcgcacca tcgccgcctc
     1021 gtccccgaag agggcctcgg ccagcgtgcg ggcgagctcg gtcttgccga cgcccgtggg
     1081 acccaagaac aggaaggagc cgatcgggcg gttggggtcc gagagtccgg cccttgccct
     1141 gcggatagcc tcggcaacgg ccgtgacggc ctcgtcctgc cccaccaccc tctcgtgcag
     1201 ctgctcctca aggcgcatga gccgctcgcg ctcctcctgg gtgagctggg agacggggat
     1261 gccggtggcg cggctcacca cctccgctat gtcctcggcc gtcacctcgg cgacggggcg
     1321 gcgggcgccg tcgcgcgacc cgtccagctc ggaccttaag ccctcgaccc ggcccttgag
     1381 ctcctgggcc cgtccgaagt cctcggccga gaccgcctgg tccttctctc ggcccaggcg
     1441 cgccacctct tcctcgagct ccttcgtgtc gcgtggcttg gtcttggagc gcagcctgac
     1501 cctcgccgaa gcctggtcca tgaggtctat ggccttatcg gggaggaagc ggtccgtgac
     1561 gtagcggtcc gagagctcgg cagcggccac gatggcctcc tccgttatct tcaccctgtg
     1621 gtgggcctcg tagcggtcct tgaggcccct gaggatgtct atggtgtcgt ccacggaggg
     1681 ctcgcccacc aggaccggct ggaagcggcg ctccagggcg gcgtccttct ctacgttctt
     1741 gcggtactcg tcgacggtgg tcgccccgac gacgtgcagc tcgccccgcg ccaaagcggg
     1801 cttgagcatg ttggaggcat ccatcgagcc ctcggcagcc cccgccccca ccactgtgtg
     1861 cagctcgtct atgaacagga tctgcccctc ggggttctcg ctcgcctcgt ctacgacctg
     1921 cttgagacgc tcctcgaact ggccgcggta ctgggtgcct gccaccagcc cggagaggtc
     1981 gagggccaag accctcctgc cgaccaaggt ctccggcacc tcgtcgttta cgatcctctg
     2041 ggcgaggccc tccacgatgg ctgtcttgcc gacgccgggg tccccgatga ggacggggtt
     2101 gttcttggtc cgcctggaga ggatctcgat ggtggtctct acttcctcgg cgcgcccgat
     2161 gacgggatct agcttgccct cacgggccat ctgggttagg tcgcggctgt actcgtcgag
     2221 ggtgggtgtg gcgctggccg gctcgcgcgc agcgcccgtg gcggtgtcta ccccgcgcat
     2281 cacggccccc ctgagcctcg tgtgcgagag tccgaaacga cccaacagtt cgcccgcctc
     2341 cgattcgtca tctctcgcca gcgcaagcag gacatgctca ggacctatgt aggaggagcc
     2401 cagagcctgc gactcctcat aggcggctaa gagcgcccgc ttggcatcgg gggccaggga
     2461 gggggtgacg tcggtgcgcc cgcctttatc ggcctcctcc tcgagctggg cccggatcgc
     2521 ttcggggtct gcctccactc cttcgagcac gcgccgcacc acattgtctt cgagcgccgc
     2581 ataaaggagg tgctcgctac tcaggtctag gttgccccac tcgacggctt gcccggccgc
     2641 acgctgcaat agttccgtcg tggagtcgtt aaagaactgg gttatgtcca cctgttctgc
     2701 ctgcctccgg gggactaccc cgcccccgcg gggagacccg cggggttcct cgtcgaagaa
     2761 cctgtcgaag aagtcgtcga agagacttcc gccccccagg ggagacctgc ccccaaaggt
     2821 cgaacgcccg accccggcct ccgcggcgtg gatctcgcag aggtgctcga ccctctggcc
     2881 ttccccggga acaacccgcc tcactgtgac gactgccggc ctgatcccgc agacgtcgca
     2941 catcctcgtg gccatctcct cttgcctcct tctcccgcct acttcgcgcg gcctgtgggt
     3001 cgcgcacgtt gtggctaggt ttggttaggg gcgaacaact tgctcaacga ggtgg
//
LOCUS       DASTUV010000173         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_974280, whole genome shotgun sequence.
ACCESSION   DASTUV010000173 DASTUV010000000
VERSION     DASTUV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974280"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="thrB"
                     /locus_tag="VFT03_03580"
     CDS             complement(<1..890)
                     /gene="thrB"
                     /locus_tag="VFT03_03580"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU4847253.1"
                     /translation="MISVRVPATSANLGPGYDAVGLALTLSMRIGLDRSPYPAIEVRG
                     TGAQLIPRGPEHPAYRAARFVAELVGESDVHFRLVQENAIPPTRGLGGSAAALVGGAV
                     AANDLFGGQIAAPDLLNIVCELDGHPDNAAPAMLGGLVIGTLTPEGVSAVRLEPKDLK
                     VVVAVPDFAVSTTAARRALPERVSHRDAAFNVGRSGLLLGALATGEYDLLRVAMQDRL
                     HQPYRSHLVPGLEDVIEAALVNGAYGACLSGSGPTVLAFAPETAARRIASAMRDAFET
                     RNVEARAWALDVDLDGAQVEP"
     gene            complement(887..1963)
                     /gene="thrC"
                     /locus_tag="VFT03_03585"
     CDS             complement(887..1963)
                     /gene="thrC"
                     /locus_tag="VFT03_03585"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU4847254.1"
                     /translation="MGVRTQEMGQGVIRRYGRWLPEIPDSARASLGEGETPLIEAQTL
                     SKRAGARLFLKFEGMNPTASFKDRGMTVALSRAVAAGSRASVCASTGNTAASASAYSA
                     RAGIKCFVVVPAGKIALGKAVQVLAHGADIVQIEGNFDEALRLSRQLAEELGDVALVN
                     SVNPDRIEGQKTAAFEVCEVLGAPPDALVLPVGNAGNITAYWKGFSEWREAGYSENAP
                     RMLGFQAEGASPLVAGHDFENPETVASAIRIGSPASKEGALAASRESGGLIESVTDDE
                     ILEAQALLASEEGVFCEPASAAGIAGLLKLVREGRGPEGTVVSVLTGHGLKDPDAVLS
                     RVELPEPVEATLEAVGERMERALR"
     gene            2238..>2908
                     /locus_tag="VFT03_03590"
     CDS             2238..>2908
                     /locus_tag="VFT03_03590"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU4847255.1"
                     /translation="MEESNRPILVTGGAGFIGGNFVLDLVGTHKVINLDALTYAGNLD
                     TLAPVAGDPGHVFVRGSIGDRPLVEGLLAEHRPRAIVNFAAETHVDRSIDGPKAFVQT
                     NVVETLELLEAALAYWQGLEGEERLGFRFVHVSTDEVYGTLGSEGFFTEETAYRPNSP
                     YAASKAASDHLVRAFHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMIYNALRGEPLPVY
                     GDGRQV"
BASE COUNT          475 a          992 c          968 g          473 t
ORIGIN      
        1 ggctcgacct gcgccccgtc caggtcaacg tcgagcgccc acgcccttgc ctcgacgttc
       61 cgcgtctcga aggcatcccg catcgcggat gctatccgcc tggccgccgt ctcgggggcg
      121 aaggccagga cggtcgggcc cgagccggag aggcaggcgc cgtaggcgcc gttcaccagc
      181 gccgcctcta tgacgtcctc gaggccggga acgaggtggg agcggtacgg ctggtgcaac
      241 ctatcctgca tcgcgacgcg caagagatcg tactcgccgg tagcgagggc gccgagcagg
      301 agccccgagc gccccacgtt gaaggcggcg tcgcggtggg agacccgctc gggcagggcg
      361 cgcctggcgg ctgtggtcga gacggcgaag tcggggacag cgacgacgac cttcaggtct
      421 ttcggctcca ggcgcaccgc gctaaccccc tctggcgtga gggtgccaat taccaggccg
      481 ccgagcatgg caggtgctgc gttgtcgggg tggccgtcga gctcgcagac tatgttgagc
      541 aggtcggggg ctgcgatctg gcctccgaag agatcgttgg ccgcgacggc cccgcccacc
      601 agcgcggcgg cgctcccgcc gagtcccctg gtggggggga tggcgttctc ctggaccagc
      661 cggaagtgga cgtcagactc tccgacgagc tcggccacga agcgggcggc gcggtaggcg
      721 ggatgctccg gcccgcgggg gatcaactgc gcgccggtgc cgcgcacctc gatggccgga
      781 tagggagacc tgtccagccc gatcctcatg ctcaaagtga gcgctagccc gaccgcgtcg
      841 tagccggggc cgaggttggc cgaggtggcg gggacgcgga cggagatcac cgcagcgccc
      901 gctccatgcg ctcgcctacc gcctccaacg tcgcttccac gggctcgggc agctccacgc
      961 ggctcaacac cgcgtcaggg tccttgagcc cgtgccccgt caggacgcta acgaccgtgc
     1021 cttcaggtcc cctcccttcc cggacgagct tcaagaggcc agcgatgcct gcggcggagg
     1081 cgggctcgca gaagacgccc tcctcgctgg cgagcagcgc ctgggcttcg agtatctcgt
     1141 cgtctgtgac gctctcgatg aggcctcccg actcgcgcga cgctgcgaga gccccctcct
     1201 tgctcgcggg cgagccgatg cggatcgcgc tcgccaccgt ctcggggttc tcgaagtcgt
     1261 ggcctgcgac cagcggggat gcgccctcgg cctggaagcc gagcattctc ggagcgttct
     1321 ccgagtatcc tgcctcccgc cactccgaga atcccttcca gtacgcggtg atgttccccg
     1381 cgttgcccac ggggaggacg agggcgtcgg gaggggctcc gaggacctcg cagacctcga
     1441 aggctgccgt cttctggccc tcgatgcggt cagggttcac cgagttgacg agggcgacgt
     1501 ccccgagctc ctcggcgagc tggcgcgaga gacggagtgc ttcgtcgaag ttgccctcga
     1561 tctggactat gtctgcgccg tgggcgagga cctggaccgc tttgcccagc gcgatcttgc
     1621 cagcggggac cacgacgaag cactttatgc cggccctcgc ggagtacgcg gaggcagagg
     1681 ccgcggtgtt gcccgtcgag gcgcagacgc tcgcgcggct ccccgccgcc acagcccggc
     1741 tcagggccac cgtcatgccc cggtccttga aggaggccgt cgggttcatc ccttcgaact
     1801 tcaagaagag gcgcgcaccc gccctcttcg agagggtctg ggcttctatt aggggcgtct
     1861 cgccctcgcc taaagaggct ctagccgagt cgggaatctc cggcagccac cggccgtacc
     1921 tcctgataac gccctgcccc atctcctgcg tccgaacgcc caatgtcaga ccttgccttt
     1981 cacgagaaat gtttgccctc cactaacgac tagcgaaccg gccggggaac gagccggcat
     2041 acgttgttcg cggcgtgcga agagaggttg gggtccgcag accatagcgc aagcaaagta
     2101 tcatgccggg gcttctcctt tcaaggagca gcgtggcgcc ttcaccaccc gtctagtaaa
     2161 ttacgctcgg aggctacagg tttcaggttt atttgctgat gcctgaagcc tgaaacgtga
     2221 aacctgaaaa ggaagctatg gaagagagca acaggcccat cctggtaacc ggcggcgccg
     2281 gctttatcgg gggcaacttt gtcctcgacc tggtggggac ccataaagtc ataaacctcg
     2341 acgctctgac ctacgcgggc aacctcgaca ccctggcccc ggtagccggc gaccccggcc
     2401 acgtctttgt gcgcggctcg atcggcgacc gcccgctggt cgaagggttg ctggccgagc
     2461 acaggccgcg ggcgatcgtg aacttcgcgg ccgagaccca cgttgaccgc tccatagacg
     2521 gcccgaaggc gttcgtccag acgaacgtgg tcgagacgct ggagctgctg gaggcggcgc
     2581 tcgcctactg gcagggtctc gagggcgagg agcgcctggg gttccgcttc gtgcacgtct
     2641 cgacagacga ggtctacggc accctgggtt ccgaagggtt cttcaccgag gagacggcct
     2701 acaggcccaa ctcgccgtac gcggcatcca aggcggcctc ggaccacctg gtgcgggcct
     2761 tccaccacac ctacgggcta ccgaccctca cgaccaactg ctccaacaac tacggaccct
     2821 accagttccc agagaagttg ataccgctaa tgatctacaa cgccctgcgc ggcgagccgc
     2881 ttcctgtcta tggcgacggg cgccaggt
//
LOCUS       DASTUV010000174         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1191509, whole genome shotgun sequence.
ACCESSION   DASTUV010000174 DASTUV010000000
VERSION     DASTUV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191509"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1679
                     /locus_tag="VFT03_03595"
     CDS             363..1679
                     /locus_tag="VFT03_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU4847256.1"
                     /translation="MEVRDVLVTGGTGRLGRRVVDRLRAEGIEPRVMSRSGRPGTIEG
                     DLLTGEGIEAAVCGADTIIHAAQSPTRKSRRTAVEGTERLLEIAARAGVSHFVYISVV
                     GLERVPLSYYREKLAAERLVERSPVAWTILRSTQFHEFVLSMVRALNRVPFVTPVPKG
                     WLFQPIDTGEVAGRLVELALELPAGRVPDIAGPEVRTLAGLTQAYLTARGSKKGVVEL
                     PIPGKAARAFREGAQAVPASAWGDITWEEFLERQGGIMQYPETPTRGGKTGMLNGLFY
                     SGPSIEALHEQYAKKGRIDQGAPVKASREFRIEAPVERVWELLSDPRGWEAWYPDVHD
                     VRLEPGVEEDARFAWANGKAHMRSRFAIVEVGREITWTGVAYGAKAVHRHVLQPTEGG
                     ATRVFSEESMAGPLLVLFYNSARLGAEMEEWLSALKIAAEAPAEER"
     gene            1853..>2384
                     /locus_tag="VFT03_03600"
     CDS             1853..>2384
                     /locus_tag="VFT03_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEU4847257.1"
                     /translation="MSGERQAGIAGYGYGTREMARSPLTLEDLDLLKRTVMFTEEDEG
                     YLRMAGDVLEGQADDILDLWYGFVGDNPHLVYYFADAQTGEPNSEYLARVRERFSQWI
                     LDTCRRRYDQVWLDYHHEIALRHTSAKKNETDGAYDTPDHIPLRYIIAFVYPITATIK
                     PFLAKGGHDSEEVDKMY"
BASE COUNT          458 a          711 c          821 g          394 t
ORIGIN      
        1 taagccccgc cgacagggag cggatagcgc acggcaacgc ggagcgtctc ctgcggctgt
       61 gatcgggagg atggaccgcc gcggtgcgag gcgcggacac gataatccat gctgcgtccc
      121 ggtgccgggc aaagcggcac gcgccttcat tgttcggctc ccggcaagcc ccgccgacag
      181 ggagcggata gcgcacggca acgcggagcg tctcttgcgg ttgtgatcgg gagtatggac
      241 cgccgcggtg cacggcgagc atcaacgatc caagaaggcg cgcctaccgc gagggaacac
      301 actttagctt accgtccggt ggtcaacccg gggaaggtaa aggaggtaag gaggttttgg
      361 tcgtggaagt gcgagacgta ctggtcaccg gcggcacggg aaggctcggc aggcgggtag
      421 tggataggct gcgtgccgag ggcatagagc cgcgggtaat gagccgtagc ggccggcccg
      481 gcaccatcga aggagacttg ctcaccggtg agggcataga ggcagcggtg tgcggcgcgg
      541 acacgataat ccatgctgcg cagagcccca cccgaaagtc acgccggacg gctgtcgagg
      601 gtaccgagcg tctccttgag atcgcggccc gcgcaggcgt ctcccacttc gtctacatct
      661 ccgtcgtcgg cctcgaacgt gtgccgctct cctactaccg ggagaagttg gccgccgaga
      721 ggctagtcga gcgctcgccg gttgcgtgga ccatcctcag atccacccag ttccacgagt
      781 tcgtcctctc tatggtgcgg gccctgaacc gcgtgccctt cgtcacgccg gtccccaaag
      841 ggtggctctt ccagcccata gacacaggcg aggtcgccgg ccgccttgtg gagctcgcgc
      901 tcgagttgcc ggccgggcgt gtccccgata tagctggccc agaggtcagg accctcgcag
      961 gccttacaca ggcgtacctc acggcgaggg gcagcaagaa gggagtcgtg gagctcccga
     1021 ttccgggcaa agcggcacgc gccttccgag aaggcgccca ggcggttccc gcctccgcgt
     1081 ggggcgacat cacgtgggaa gagttcctcg agcgacaagg agggatcatg cagtacccgg
     1141 aaacaccaac acgtggagga aagaccggaa tgctgaacgg gctgttctac agtggcccgt
     1201 cgatcgaggc gttgcacgag cagtacgcga agaaagggcg catagaccaa ggggcaccgg
     1261 tcaaggcgtc ccgggagttc cgcatcgagg cccccgtcga gcgggtgtgg gagctgctga
     1321 gcgaccctcg cggctgggag gcctggtacc cggatgtcca cgacgtccgt ctcgagcccg
     1381 gggtggagga ggacgcccgt tttgcctggg cgaatggcaa ggcgcacatg aggtcgaggt
     1441 tcgccatcgt cgaagtcggg cgggagatca cctggaccgg tgtggcctac ggggccaagg
     1501 cggtccaccg acacgtcctg cagcccaccg aaggcggcgc gacgcgcgtc ttctccgagg
     1561 agtcgatggc cggacccttg ctcgtgctgt tctacaacag cgccaggctg ggggccgaaa
     1621 tggaggaatg gctgtcagcg ctcaagatcg ccgccgaggc gcccgcggag gagcgatgat
     1681 cggccaagct ttccactggg tgaacctcgc gcttgccttt ttgctggagc tgtgtgctct
     1741 aggcgctttg ggacgcatgc tctttgacat ggtcaacaat cccatcttat agttaggact
     1801 cctgacaaat tgctcggggc aatcggctct gagccgggag aaaggggtgg tgatgagcgg
     1861 tgagcggcag gcgggcatcg ccgggtacgg ttacggtaca cgagagatgg cgaggtcgcc
     1921 cctgacctta gaggatctgg accttctcaa acggacggtt atgttcaccg aggaggacga
     1981 ggggtacctg aggatggcgg gcgacgtgct cgagggccag gccgacgaca ttttggactt
     2041 atggtacggc tttgtggggg acaacccgca cctggtctac tatttcgccg acgcgcaaac
     2101 cggggagccg aactccgagt atctggcgcg cgtgcgcgag cggtttagcc agtggatcct
     2161 cgacacctgt cgccgccgtt atgaccaggt gtggctcgac taccatcatg agatcgccct
     2221 caggcacact tcggccaaga agaacgagac cgacggggcc tacgacacac cggaccacat
     2281 accgctgcgc tacataatcg ccttcgtcta cccgataacg gcgaccataa agccgtttct
     2341 cgccaaaggg ggtcacgact ctgaagaggt cgacaagatg taca
//
LOCUS       DASTUV010000175         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1578361, whole genome shotgun sequence.
ACCESSION   DASTUV010000175 DASTUV010000000
VERSION     DASTUV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578361"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VFT03_03605"
     CDS             complement(<1..1016)
                     /locus_tag="VFT03_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU4847258.1"
                     /translation="MVHPSPGSGDVARSEAPTVSGPLAVRICLLGGFRVSVGDKEIEG
                     SAWRLKKAAALVKLLALVPGHRLHRERVQDLLWPDLGKHAASNNLRQCLYAARRTLGT
                     ESADASRYIRSRDEQILLCPEERLWVDVEAFEEAGAAARRARDPAAYRAALDLYAGDL
                     LPDDLYEAWAEERRTELRRSCLALLAELAGLYEERGELEAAAEALRRAVVHDPTSEEA
                     HVGLMRLYALSGRQGSAIAQYERLRDVLSDRLGTEPGVAARDLRDEIAVGNLSPARTD
                     PSPESPAAADKHNLPAPRTSFVGRERDLVEVKRALAMTRLLTLTGAGGAGKTRLAQEV
                     ARDLV"
     gene            complement(1304..1540)
                     /locus_tag="VFT03_03610"
     CDS             complement(1304..1540)
                     /locus_tag="VFT03_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847259.1"
                     /translation="MYLRTREFAATPEPTSIRPTMTMGTASGGPPVFLRAPPAVLGSA
                     LALAVGLALADAPAVALALAVALAVALALALPPE"
     gene            complement(1646..2056)
                     /locus_tag="VFT03_03615"
     CDS             complement(1646..2056)
                     /locus_tag="VFT03_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4847260.1"
                     /translation="MNEQENLQVVHQAYEAFGRGDVPGVLAMLTEDVEWSTPGPPDVI
                     PYAGTRTGHEQVAGYFGAFGDAVEVSEFEPQRFFAEEEMVIVLGHYAFRVKSTGKPVD
                     TDWVHAFTFSDGKIARFRGYEDSAAVAAAFEART"
     gene            complement(2333..2635)
                     /locus_tag="VFT03_03620"
     CDS             complement(2333..2635)
                     /locus_tag="VFT03_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEU4847261.1"
                     /translation="MYVVIWRFRPLTGREHEFERAYGPSGEWTRLFQHGEGYLGTELL
                     QSSDDPREYLALDRWASGAAYEVFRARYGSEYRRLDRRLEELTEEEALLGAFETLS"
BASE COUNT          468 a          942 c          845 g          470 t
ORIGIN      
        1 acgaggtccc tggcaacctc ctgagcgagc cgtgtcttgc cggccccccc ggcgcccgtc
       61 agggtcagga gccgggtcat ggccagggcg cgtttgacct ctacgagatc tcgctccctt
      121 ccgacgaagc tggtccgcgg cgcggggagg ttgtgcttgt cggcggctgc cgggctttcg
      181 ggggaaggat cggtccgcgc cggcgagagg ttgcccacgg ctatctcgtc tcgcaggtcg
      241 cgggcagcga cgcctggctc tgtgcccagc cggtcggaga gcacatcgcg caaccgctcg
      301 tactgcgcga tggcggatcc ctgccgaccg gagagcgcgt aaagccgcat caaaccaacg
      361 tgcgcctcct cgctggtggg atcgtggacc accgcccgcc gtagcgcctc ggcggccgcc
      421 tcgagctccc cgcgctcctc atacagcccc gccagttcgg cgagcagcgc gaggcaagac
      481 cgccgaagct cggtccgcct ctcctcggcc cacgcctcgt agaggtcgtc aggcaacagg
      541 tcgcccgcgt acaggtcgag cgccgccctg taggctgcgg ggtcccgggc gcgacgcgcg
      601 gcggcgcccg cctcctcgaa cgcctccacg tcgacccaga gccgctcctc cgggcagagg
      661 agaatctgct catcccgaga ccggatgtac cgggaggcgt cggcagattc cgtgcccaag
      721 gtccggcgcg cggcgtacag gcactgcctg aggttgttcg aggcagcgtg cttccccagg
      781 tcgggccaca acaggtcctg cacccgctcg cggtggaggc ggtggccggg gacgagggct
      841 agcaacttca ccagagcagc cgccttcttc agccgccagg cgctcccctc gatctccttg
      901 tctcccactg agaccctgaa gccgccgagc aggcagattc ggaccgctag gggccctgag
      961 accgtcgggg cctcggatct cgccacgtca ccagagcctg gagaaggatg caccatgctc
     1021 tactccttgc ggtttccgtg gcttattttc ctacagaacc tggatgcatg acaacgaact
     1081 ctggcgcacc ggcgccaatt cccttgtcca cgggcacacc caacacggga ctccttgaca
     1141 cggactgaac aaggcaccaa gaatggtgtt ctgacaagag gcgcctgccc ttcgcggcgc
     1201 gcccgatcta gaggaggtgc aaggtgaaaa gaatgtcgat tttgctagcc gtgagcgcga
     1261 tgatggtgct ggtcgcggga tctgccgtac tggcgcaacc gtactactcg ggcggaagcg
     1321 cgagcgccag cgcaactgcc agcgcaactg ccagcgcgag cgccacggca ggcgcatcag
     1381 cgagtgccag ccccacggcg agcgccagcg cgctgcccaa gaccgccggc ggtgccctga
     1441 ggaagaccgg cggtcctccg ctggccgtac ccatcgtcat ggtaggcctg atgctggtcg
     1501 gttccggggt cgcggcgaat tctctcgtgc ggaggtacat ctcccggtaa acccgcttcg
     1561 gaaggtgagg agataccaag ggcggccacg ggacgggaag aaaggttctc gccgcagccc
     1621 tcggctttcc cccatcccct cgcttctacg tgcgggcttc gaaggcagcg gccacggcgg
     1681 cgctgtcctc gtaaccgcga aagcgcgcga tcttgccgtc gctgaacgtg aaagcatgca
     1741 cccagtcggt atcgaccggc ttgcccgtgc tctttacacg gaaggcgtag tgccccagca
     1801 cgattaccat ctcctcctcg gcaaagaacc tttgcggctc gaactccgag acctccactg
     1861 catcgccgaa agccccgaag tacccggcga cctgttcgtg acccgtgcgc gttccagcgt
     1921 aggggatcac gtcgggcggg ccgggtgtgg accactcgac gtcctcggtg agcatggcga
     1981 gcactcccgg cacatcccct cgcccaaagg cctcgtaggc ttggtggacc acctgaaggt
     2041 tctcttgttc gttcacgtgc ccgacctccc ttcctggcct gacgccgact ggtcttgcct
     2101 gcctactgtc agactagcgg cacctagagc gaaaagccag gaaaggagca acaacggctg
     2161 cccgccggtt cccggtagcg tccccccttc ctgatggtct ccgggatgac gtcgtccttc
     2221 ctgtcgctat agggcagtca actactcctc tgtccgaggc agaggaattc tcggcgcctc
     2281 cggtacatcc tcgtgagagc ccacccctat tcatttcaag agatcgaggt ctttatgaga
     2341 gtgtctcgaa ggcgcccagc agtgcctcct cctccgtgag ctcttccaac cgacggtcca
     2401 gccgtcgata ctcacttccg taccgagctc ggaatacttc gtaggcggcg ccagaggccc
     2461 agcggtccag agccaggtac tctctgggat cgtcggagct ctggagtagt tcggtgccga
     2521 ggtagccctc gccgtgctgg aacaaccgtg tccactcccc ggatggaccg taggctcgct
     2581 cgaactcgtg ctcccgaccc gtgaggggcc ggaaccgcca gattacgaca tacatagtca
     2641 gtctcctcaa caagagggcc aggccatgaa ggcgtccctc acaggtcgaa ggtcaacgtt
     2701 agcctacgtt acctcacagg cctcc
//
LOCUS       DASTUV010000176         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_779418, whole genome shotgun sequence.
ACCESSION   DASTUV010000176 DASTUV010000000
VERSION     DASTUV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779418"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFT03_03625"
     CDS             complement(<1..444)
                     /locus_tag="VFT03_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU4847262.1"
                     /translation="MPELVLGPLLRHVGPSDATFWVETDSPCEVEVRAGRPSTRSPTF
                     RVGTHHYALVHVTGLTPGVSHEYEVRLDGEVVWPEPSSPFPPSVVRVPGEGELVKLVF
                     GSCRMSAPHEPPFTLSYADDERGLGVDALYAMAMQLRDQPPEGLPH"
     gene            complement(514..1533)
                     /locus_tag="VFT03_03630"
     CDS             complement(514..1533)
                     /locus_tag="VFT03_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847263.1"
                     /translation="MTLLTIVEFVVALVAILIGAAFFTNAVEILGSRLGMGQGAVGSL
                     LAAVGTALPESMIAIVAILEPVVSGGDSEGGAEIGVGAILGAPFMLATLALFVVGASA
                     LAFARRRPQGATLGADGATIRRDLRYFLVFFALAAAVGLVALPLWLKAVVAGLLVVGY
                     ARYVRLTLVSGGHLEDVPETLLALPRRPNPPLLAVTAQVLASLGVIVAGAHFFVDAVE
                     HAAEGLGLPAGLIALVLAPLATELPEKFNSIFWIRDGKDTLALGNITGAMLFQSTIPV
                     SFGILFTPWNLDFLSLFATVLALASGTLVYLSLERSGKLGAGLLMVGGLFYLAFVVGV
                     VLVIA"
     gene            1918..>2054
                     /locus_tag="VFT03_03635"
     CDS             1918..>2054
                     /locus_tag="VFT03_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU4847264.1"
                     /translation="METKRRVFSGIQPSGTPHLGTYLGALKNWVSIQDEYVCNYCIVD
                     L"
BASE COUNT          397 a          702 c          662 g          293 t
ORIGIN      
        1 gtgcggcagc ccctcaggcg gctggtcccg taactgcatc gccatcgcgt agagggcgtc
       61 aaccccgagc ccccgctcgt catccgcgta gctcagagta aacggcggct cgtggggagc
      121 tgacatcctg cacgacccga agacgagctt gacgagctct ccctcgccag gaacgcgcac
      181 gacgctgggc gggaagggcg agctcggctc cggccacacc acctcaccgt ccagcctgac
      241 ctcgtactcg tggctgactc cgggcgtgag gcccgtgacg tgcacgagcg cgtagtggtg
      301 cgtgccgacg cggaaggtgg gcgagcgggt cgatggtctc ccggcgcgca cctcgacctc
      361 gcaaggggag tccgtctcga cccagaaggt tgcgtcggag ggtccgacgt gtcgcaggag
      421 aggcccgagg acgagctcag gcacggcggt tcgcgatttt gcgcgagcat agcaagacgg
      481 ggccatggta cacgatgcgg ctgcgcgggt ggatcaggcg attaccagca ccacccccac
      541 gacgaacgcc aggtagaaga gcccaccgac catcaggagc cccgctccga gtttacccga
      601 gcgctcgagc gagaggtaca cgagcgttcc ggaagcgagg gccagtaccg tggcgaagag
      661 gctcaaaaag tccaggttcc agggggtgaa gaggatgccg aaggagaccg ggatagtgct
      721 ctggaagagc atcgcccccg ttatattgcc cagagcgagc gtatctttgc cgtccctgat
      781 ccagaagatg gagttgaact tctccggcag ctcggtggcg aggggggcga ggacgagcgc
      841 gatcaagccc gccggcaacc ccagcccctc cgccgcgtgc tccacggcgt ccacgaagaa
      901 gtgcgcccct gcgacgatta cgccgaggct cgccagcacc tgagcggtga cggcgagaag
      961 cggcgggttc ggcctccgag ggagagccag cagcgtctct ggcacgtcct ccaggtgccc
     1021 gccggagacc agggtcaacc ggacgtaacg tgcgtagccg acgaccagca gccccgccac
     1081 gaccgccttc aaccagagcg gcagcgccac cagccccacc gccgccgcaa gagcgaagaa
     1141 gacaaggaag tacctcaagt cgcgcctgat cgtcgcgccg tccgcgccca gcgtcgcgcc
     1201 ctggggccgg cgccgcgcaa aggccagcgc cgaggcgccg acgacgaaga gggcgagggt
     1261 cgccagcatg aacggcgcgc ccaggatcgc cccgacccct atctcggcgc ccccctccga
     1321 atccccgccg gagacgacgg gctcgaggat ggccactatc gcgatcatgc tctccgggag
     1381 cgcggtcccg acggccgcga gcaggcttcc cacggccccc tgtcccatcc ccagccggga
     1441 cccgaggatc tcgaccgcgt tcgtgaagaa ggccgctccg ataaggatcg cgaccagcgc
     1501 aacaacgaac tcgactatcg tcaacaacgt catcccttcc cttctctgag aaccgtcccg
     1561 ctaaagaagc ccctgggcac gttcatgcgc caaccccgtc cacgggaagg gccagctcgg
     1621 ggaagtgaac tgaagagggg ccgtgattcg gccggtgcgc gcatcgggac ggtgcctgcc
     1681 actcgagact ccttcggctc gcctaactac aagccggaag tctctcccgc ccgtgcgcag
     1741 gacgtgctcc accgggctct cgccgtaatg tcgatggagc ctcgaaggat actcccctcc
     1801 gggtcgcaca agagtttact ccaacccgcc gagccttcgt tgtttgttcg ttcacaaatt
     1861 gatctgccgg cgagcgtacg gttcgccatc cacagcgcgt ccgttagaat gggtctcatg
     1921 gagacgaaac ggcgggtatt cagcggcatc cagcccagcg gcacgccgca cctggggact
     1981 tacttgggtg ccctaaagaa ctgggtgtcc atacaggacg agtacgtctg caactactgc
     2041 atcgtggacc tgca
//
LOCUS       DASTUV010000177         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_758831, whole genome shotgun sequence.
ACCESSION   DASTUV010000177 DASTUV010000000
VERSION     DASTUV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758831"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1952)
                     /locus_tag="VFT03_03640"
     CDS             complement(132..1952)
                     /locus_tag="VFT03_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4847265.1"
                     /translation="MREQEQRQQEVLCGEGDGGALAQGGSGILEAIVEGTADSVFAKD
                     LEGRYLMINSSAARILGMPKEEVVGKDDAQLLPPETARRLAEADSRVLATGESSVYEE
                     VLPVAGQSRTYLSTKGVFRDAGGNVAGIIGISRDITDRKRAEVALRRSEERFRGLAEG
                     IDFIPWEADLATWRFTYVGPQAVKILGYPVEDWYRDGFWVDHIHPEDRVWVADYCATN
                     SATRRHYRFEYKMLASDGRTVWLDDIVSVTEDEGGTKLLQGVMVDVSERKRMEEELRR
                     SEERFRSLVQNASDVITVVDAEGTILYVSPAVRRVLGYEPEEMIGEGALAFGHPDDRE
                     QAMRLLAELSARPGIQPTFELRVPHKDGSWRYLEHTANNLLDDPSVGGIVINQHDVTE
                     RKRAQAAEFEIREAERRRISRDLHDGVLQDLALVTQRLEMKRLVDESDGSDDEFEPEI
                     DSLRGAVRGLRAAIYELRPYEAVPFIREVESMARHERELTPEREIGVVVEDGFPAELP
                     EAEATQLQRLIREALTNARRHSAARHIQVLLGATEDELWAEVSDDGRGIEPGWSGGGL
                     GLSTMKERATQLGGNLEIRSEPGTGTRVRLRMPHPVPPRS"
BASE COUNT          323 a          736 c          618 g          385 t
ORIGIN      
        1 atacgggcga tgggactaga atctccagta tagacccctt aactggcgaa cctactctta
       61 cgcagtagaa gtacacacaa ggtaagcgga ggctttacgg cagggcctcc gtctacctta
      121 tagaggatgg gtcacgatcg cggcgggacc ggatgcggca tccgcaggcg taccctcgtc
      181 cccgtgccgg gctcggaccg gatctccagg ttcccgccca gctgcgtcgc ccgctccttc
      241 atcgtggaga gccccaaccc cccgcccgac catccgggct cgatgcctct accgtcgtct
      301 gagacctccg cccatagctc gtcttcggtc gcccccagca acacctggat gtgccgggcg
      361 gcggagtggc ggcgggcgtt ggtcagcgcc tcgcgtatga ggcgctgcag ttgggtcgcc
      421 tccgcctcgg ggagctcggc cgggaaccca tcctcgacaa ccacgccgat ctcgcgctcc
      481 ggggtcagct ctcgctcgtg gcgcgccatg gactccacct cccggatgaa cggcaccgcc
      541 tcgtaaggcc gcagctcgta gatggccgcg cgcagccccc ttaccgcccc acgcaggctg
      601 tctatttccg gctcaaactc gtcgtctgag ccgtcgctct catctacgag ccgcttcatc
      661 tccagccgtt gtgtgaccag agcaagatcc tggagaaccc catcgtgcag gtcgcgggag
      721 atgcggcggc gttcggcttc ccggatctcg aactcggccg cctgggcgcg cttgcgctcg
      781 gtgacgtcgt gctggttgat gactattccc ccgacgctcg ggtcgtcaag caaattgtta
      841 gcggtgtgct cgaggtagcg ccacgagccg tccttgtgcg gaacccgcaa ctcgaaggtc
      901 ggctggatcc ccggcctggc agaaagctcg gcgagcaggc gcatcgcctg ctcccggtcg
      961 tccgggtggc cgaacgcgag ggcgccctcg ccgatcatct cctccggttc gtagccaaga
     1021 accctcctga ccgccgggct gacatagagg atggtgccct ctgcgtcgac gaccgtgatg
     1081 acgtccgagg cgttctgtac cagggagcgg aaccgctcct cgctcctacg caactcttcc
     1141 tccatgcgct tgcgctcgga gacatcgacc atgacccctt gcagtagctt cgtcccaccc
     1201 tcgtcctccg tcacgctcac gatgtcgtcc agccacacgg tccgtccgtc tgaagcgagc
     1261 atcttgtact cgaagcggta gtgacggcgc gtggccgagt tcgtcgcgca atagtcggcc
     1321 acccacaccc ggtcctccgg atggatgtga tcgacccaga aaccgtcgcg gtaccagtcc
     1381 tccacagggt agccgaggat cttgaccgcc tggggtccca cgtaggtgaa ccgccaggtg
     1441 gcgaggtcag cctcccaggg gatgaagtcg atcccttcgg ccaacccccg gaaccgctcc
     1501 tcgctgcgac gcaatgctac ttcggcgcgc ttgcggtcgg tgatgtcgcg cgagatgccg
     1561 atgatgcccg cgacgtttcc tcctgcgtct ctgaagacgc ccttcgtcga gaggtacgtg
     1621 cgggattggc cggccaccgg cagcacctcc tcgtagacgc tggattctcc ggtcgccagg
     1681 acccggctgt ctgcctcggc gagccggcgg gcggtttcgg gtgggagcag ctgcgcgtcg
     1741 tctttgccga cgacctcctc cttcggcata cccagtatcc gggcggcgga ggagttgatc
     1801 atcaggtagc gaccctcgag atccttcgcg aagacggagt cggcagtccc ctcgactatt
     1861 gcctcgagga tgcccgaccc cccctgcgca agcgcccccc cgtcgccctc accgcacagc
     1921 acttcctgtt gccgttgctc ctgttcacgc atggcctgcc taccttgtcg gctagatcgc
     1981 ggccaacata ttgccacccg acgcggcgac gtgcacgcgt cttcctaagc tagcgtagct
     2041 agcgccccct cgccgcaagc tc
//
LOCUS       DASTUV010000178         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1037436, whole genome shotgun sequence.
ACCESSION   DASTUV010000178 DASTUV010000000
VERSION     DASTUV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037436"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFT03_03645"
     CDS             <1..1038
                     /locus_tag="VFT03_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4847266.1"
                     /translation="GRPLFRQGVLLDITDRKEAEEQVLFQAHLLEQVQAAVIATDLRG
                     RVTHWNEYAERLYGWSREETLGRDIRELTVSPDQIPGAKEILRRILGGETWEGEFVVR
                     RKDGSRFLARVTDSLIRDAEGRAVGIVGVSTDVTVRKRLEEGLREIREAERRRIARDL
                     HDVVLQDLAGALQGMQAAQVESAGSGLEQEIAALRRAVGSLRNAIYDLRLEKEQPFVR
                     AVESLVELNRQLTPEREIMLTVCDGFPSELPGAADVELLRVLQEALVNVRRHSCARCV
                     EVVLSAGHRRVRAEVADDGVGFDPASVREGVGLSGMRERVSALGGRLEIVSTPDQRTS
                     VKVEMPLTGSP"
     gene            complement(1035..1709)
                     /locus_tag="VFT03_03650"
     CDS             complement(1035..1709)
                     /locus_tag="VFT03_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4847267.1"
                     /translation="MDADETIRVAIADDQRLFAHGLARIIGVQPGMEVVGEAHTGEEA
                     VSLCLDVEPDVVLMDLSMPVMDGVSATRKILGLLPRTRVLILTVHTDDAHVFQGIKAG
                     AEGYILKDCTPEDLASAIRTVHAGDTIMAQDIAKKTLSTFEGIRSEAESIAPRLTERE
                     LEVVRALARGKSNKEIAKSLGISEKTVRNHASNIYKKLHIFDRTQAVIYAIRQGLVDV
                     EELEPR"
     gene            1924..>2166
                     /locus_tag="VFT03_03655"
     CDS             1924..>2166
                     /locus_tag="VFT03_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU4847268.1"
                     /translation="MNRKLTRRRFLGLAGAGVAVTALLAAYLVRRKPDQLSGSIIEVA
                     RGVSSEPHRIGSFIVMLETGRGSSDVVLSVTHSSRPE"
BASE COUNT          381 a          671 c          722 g          392 t
ORIGIN      
        1 ggccgccccc tgttccgcca gggcgtgctg ctcgacataa ccgatcgtaa agaagccgag
       61 gagcaggtgc tgttccaggc gcacctgctc gagcaggttc aggccgccgt catagccacc
      121 gacctgcgcg gcagggtgac gcactggaac gagtacgccg agaggctcta cggctggtcg
      181 cgcgaggaga ccctgggccg agacatcaga gagctcaccg tcagccccga ccagatcccg
      241 ggcgccaagg agatactgcg gaggatcctt ggcggggaga cgtgggaggg agagttcgta
      301 gtgaggcgca aggacggctc gcggttcctg gcccgcgtca ccgactccct gatccgggac
      361 gccgaggggc gcgcggtcgg gatcgtaggc gtctcgacgg acgtaacggt gcgcaagcgg
      421 ctcgaggagg gcctgcgcga gatccgggag gccgagcgcc gccgaatagc acgcgacctg
      481 cacgacgtgg tcctccagga cctcgcgggc gccctgcagg ggatgcaagc ggcgcaggtg
      541 gagtctgccg ggtccggtct ggagcaggag atagccgcgc tcaggagggc tgtcgggagc
      601 ctgcgcaacg ccatctacga cctccgcctc gagaaggagc aaccgttcgt gagggccgtc
      661 gagtccctcg tggagctcaa ccgccagttg acgccggagc gcgagatcat gctgacggta
      721 tgcgacggct tcccgtccga gcttcccggc gccgcggacg tggagctgtt gcgcgtcctc
      781 caggaggccc tggtcaacgt ccggcgccac tcctgcgcgc gctgcgtcga ggtcgttctc
      841 tccgccggcc accgcagggt gcgagccgag gtcgctgacg acggagtggg cttcgaccct
      901 gcctcggtcc gtgagggcgt gggcctctcg gggatgcgcg agcgagtttc ggcgcttggc
      961 gggaggctgg agatagtgag cacgcccgac cagagaacca gcgtgaaggt ggagatgccc
     1021 ttgactggct cgccctagcg gggctccagc tcctccacgt ctacgagccc ttgccttatg
     1081 gcatagatga cagcctgtgt cctgtcaaag atgtgcagct tcttgtagat gttcgaggcg
     1141 tggttcctta cagtcttctc cgagatcccc aggctcttcg ctatctcttt gttgctcttg
     1201 cccctcgcca acgccctcac gacctccagc tcccgctccg tgagcctcgg cgctatgctc
     1261 tccgcctcag agcgtatccc ctcgaacgtc gagagcgtct tcttggcgat gtcctgagcc
     1321 attatggtgt cgccggcgtg gacggtacgg atggcgctgg cgaggtcctc aggagtgcag
     1381 tctttgagga tgtagccctc agcgcccgcc tttataccct ggaatacgtg agcgtcgtcc
     1441 gtgtggacgg taaggatgag gacgcgggtt ctgggcagca ggcccaggat cttgcgtgta
     1501 gcgctaacgc catccataac gggcattgag aggtccatca acaccacgtc cggctcaacg
     1561 tccaggcata gggagaccgc ctcctcacct gtgtgagcct ctcccaccac ttccatgcct
     1621 ggctggacac cgatgatcct ggcgagcccg tgggcgaaga gccgctggtc gtcggcgatg
     1681 gctactctga tggtttcgtc tgcatccaag cgagcgttta tcttaaacct tttctagcct
     1741 cactgcccgg ccaagcacaa atctaggcgc cccgggggtc gacagagcgc cggcgcgaaa
     1801 caccacacga ggccacgccc gaaaggcgag atctccgcct gctgtatgat gcgggagagc
     1861 gtggtctggg gccgataagt acactctgca ctgagtacgc gagacaagtc ggaagagacc
     1921 cgagtgaaca ggaagttgac gaggaggaga tttctaggcc tcgccggagc cggtgtggcg
     1981 gtaacggccc tgttggctgc atacttagtc aggagaaagc cagatcagct ctcgggatcc
     2041 attatcgaag tggcgcgggg cgtctcttcc gaacctcaca ggatcggctc gttcatcgtt
     2101 atgctagaga ccggtcgagg gtcttcagac gttgtactga gcgtcactca cagctccagg
     2161 cccgag
//
LOCUS       DASTUV010000179         8076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_968836, whole genome shotgun sequence.
ACCESSION   DASTUV010000179 DASTUV010000000
VERSION     DASTUV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968836"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFT03_03660"
     CDS             <1..488
                     /locus_tag="VFT03_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847269.1"
                     /translation="FGASLRALYGPEKATIRYSYSAHLDEYAEALREAHSTDIERGTT
                     SVGPHRDDFEVLLGGVNLTTYGSQGQQRLATLALKFAARDHVREEVGEDPILLFDDVM
                     SELDERRREYLAGYFLESTQAVITTTNLDYFDEEILERAHVVRISGGAVSETTSGGVR
                     N"
     gene            642..2582
                     /gene="gyrB"
                     /locus_tag="VFT03_03665"
     CDS             642..2582
                     /gene="gyrB"
                     /locus_tag="VFT03_03665"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU4847270.1"
                     /translation="MGTKAASKRSKSTYDASSIQVLEGLEAVKRRPGMYIGSTGPRGL
                     HHLVWEIVDNSIDEALAGYCSEILVTVHGDNSVSVSDDGRGMPVGKMDKYGKSAAEVI
                     MTTLHAGGKFDGQGYKVSGGLHGVGASVVNALSEWLEMEIRREGHVWTQRYERGFPKG
                     DLAKARKLKRGEGTGTTISFRPDPEVFTETVELSFDTLSRRFRESAFLNKGLKIRLVD
                     EREEGREVAYQYEGGIRDFVAHINEAKDPVHKTVFYFQREDEGVGNVEVAMQWNNGFQ
                     DSVFTFANNINTHEGGAHLSGFRAALSRTINAYARGKGLLKEKEENLTGDDIREGLAA
                     VISVKMRDPQFEGQTKTKLGNTEIKGLVESSTNRFLAEFLEERPAEAKAIINKALQAA
                     RARQAARKVRDTIRKGYLESSTLPGKLADCSSKDPARSELYIVEGDSAGGSAKQGRER
                     NFQAILPLRGKILNVEKANINKVLSNVEIQAMISAIGAGVGETFDLGQARYNKVIIMT
                     DADVDGSHIRTLVLTFLYRNMKELIEAGYVYIAQPPLYKITHQRKDYYVYNDRELQET
                     LTRLSANGNASIGRFKGLGEMNPNQLWETTMDPQNRTLLQVTVESAAVADELFTDLMG
                     DKVEPRKRFIEENARKVKNLDA"
     gene            2684..5239
                     /gene="gyrA"
                     /locus_tag="VFT03_03670"
     CDS             2684..5239
                     /gene="gyrA"
                     /locus_tag="VFT03_03670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU4847271.1"
                     /translation="MLDTFSGNIRPHNIEDEIKDSFLSYAMSVIVSRALPDVRDGLKP
                     VHRRVLYAMHDLGLQPNRPYRKSATVVGEVIGKYHPHGDSAVYDTLTRLAQDFSMRYP
                     LVDGQGNFGSVDGDPPAAMRYTEARLMRMATEMLRDISSDTVDFVPNFDESQRQPEVL
                     PARFPNLLVNGSDGIAVGMATKIPPHNLGEVVDATVALIDDPDLDDKNIARHIKGPDF
                     PTGGVIVGLRGIRDAIETGRGSVRVQAKAHTEQIKGNRTQIVVTEIPYQVNKSYLLQK
                     IAELVKERKLTDISDLRDESDRNGMRIVIELKREAVPKVVLNNLYKHTQMQQSFGVNL
                     VALVDGVPRTLSYREVLKHYVAHQFDVVTRRTRYELERARARAHILEGLIIALGNLDE
                     VVAIIRKSRSVETARKNLIKAFKLSERQAQAILDLRLQRLTALERQKVEQEHRDLVEK
                     IEYLESVLADEAKVYEIIKEELLEVRAAYADERRTQITAEEGADFEIEDLIAEEEMVI
                     SISNGGYLKSVPVNTFRKQGRGGVGVAGMELKEGDYIEHLFITTTHHYMLFFTNKGKV
                     YRLKVHQLPRMGRTAKGRHVANLLPLAQGETIAAVIQTKEFKEAQYLLFATRKGIVKK
                     TAFMEYNTPLKNEGIIAINLADDDELIDVRHASDGASVVIVTRGGMGIRFSQDEARPM
                     GRATAGVKGITLKKGDDVLSMDVVSEAGGATADLFMITEQGYGKRTALSEFRSQGRGG
                     QGVIAMKTSRDRGRLAGVRVVRPDLHELVIVSNVGTTIRIDAESVSRQGRAAQGVRVM
                     NLRAGDGVSALAKVVSSRGTDAEGATDELSLDELVNEPAATDGASPNGGVRRG"
     gene            5232..5645
                     /locus_tag="VFT03_03675"
     CDS             5232..5645
                     /locus_tag="VFT03_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847272.1"
                     /translation="MDKPCDGVVECTALVLAESCVEFEGRLTLFGVLDEIRPAEEGET
                     SFVVYAKFEGCEHASDRERRASILITDEDGEVLMESDDYSIWLEGAEEPVAIVAAFDL
                     PEDFGDEERVLWIEAVLDDDILSQTVVRLHDRYSV"
     gene            5717..6895
                     /locus_tag="VFT03_03680"
     CDS             5717..6895
                     /locus_tag="VFT03_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU4847273.1"
                     /translation="MTEDLAVFIDWENIYISTVSEYNSKPNVSAILEKAREYGRIVSA
                     TAYADWTDGDFRDAPPTLYSNGISPRYISARYFPGGRSQKRRTNSIDVMLAVECADFL
                     HTHPQVDTYVLVTGDGDFIPLVSLLRSRGKTVVVLGVSEATSYHLIESADHFISYASL
                     LEEERAARARDRQPKKKAADPYKELVRAVEALKKGDKTRVLGQVKQQMIVQLGSFDER
                     NVGFKKFKDFVVEAERRGLVNTVDQDLELQVYLPDESVPDLTGSDLAAEEPKPDGERA
                     EARANGRRDAEPPEDGHGLDLLEDLTPYELRTITRSVASLRQPASFVEIFGSIRDLDE
                     RGELELSREEIEEVIGAMLDADYLSRVREKPYKEAKADMTFYALNRDREEVKNALNGR
                     "
     gene            complement(6909..7199)
                     /locus_tag="VFT03_03685"
     CDS             complement(6909..7199)
                     /locus_tag="VFT03_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847274.1"
                     /translation="MEILLQIGVEVLRLIPLILAFYVPALLGVAIWSERGPGYRPKAV
                     LMLVLGFGAVIAVQLMFRSVSAAQVAETVGLSLVQISAALALAALTVYKLAD"
     gene            7299..7679
                     /locus_tag="VFT03_03690"
     CDS             7299..7679
                     /locus_tag="VFT03_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847275.1"
                     /translation="MGINQRVSLRKFVDDIRRWVEEGRSDEWIASALGTSPSSVQSFR
                     SRNAIYRRGAGSTLHHPEDYASYEGAIDEEGPGVWFDPAVGDDPRWQKRWGETERVEL
                     RLTPTRIVLLRRSGSRARRLQPSA"
BASE COUNT         1708 a         2485 c         2656 g         1227 t
ORIGIN      
        1 acttcggagc ctctctaagg gctctatacg gccccgagaa ggccacgatc cgttattcct
       61 atagcgcaca cctcgacgag tacgcagagg cgctcagaga ggcgcacagc accgacatcg
      121 agcgtggcac cacctcggtc ggaccgcacc gggacgactt cgaggtgctg cttggcgggg
      181 tgaacctgac gacctacggc tcacagggcc agcagcggct cgcgacgctc gcgctcaagt
      241 tcgccgcacg cgaccacgtg agggaagagg taggggagga ccccatactg ctcttcgacg
      301 acgtcatgag cgagctcgac gagcgccgcc gcgagtatct ggcagggtat ttcctcgaga
      361 gcacccaggc cgtcatcacc accaccaacc tcgattattt cgacgaggag atactggaga
      421 gagcccacgt cgtacgcata tctggtggtg ccgtctcgga gactacaagc ggtggtgttc
      481 gaaactaggg cggcgtcgcc ggaaatggct taaataagcg gcaaacgctg ccaagagggg
      541 ttggaaagcg cacaagatgt gttacaatct tggggtgcga gcctttaccg ataagaacgt
      601 tttcgcaact acagcagatt ataagacgag gagggtcctt tttggggact aaagcggcgt
      661 ccaagcgctc caagagcacc tacgatgcaa gctccatcca ggttctggag gggctcgagg
      721 ccgtcaagcg gcggcctggc atgtacatag gctctacggg gccgcgcggg ttgcaccacc
      781 tcgtgtggga gatcgtggac aactccatag acgaggctct ggcggggtac tgctcggaga
      841 tcctggtgac tgtccacggg gacaactccg tctccgtctc ggatgatggg cgcgggatgc
      901 cggtaggcaa gatggacaag tacggaaagt ctgcggcgga ggtcatcatg acgacgctgc
      961 acgccggcgg caagttcgac ggccagggct acaaggtctc cggcgggctg cacggcgtgg
     1021 gcgcctcggt cgtcaacgcg ctcagcgagt ggctggagat ggagatccgg cgcgagggcc
     1081 acgtctggac ccagcgctac gagcgcggct tcccgaaggg agatctcgcc aaggcccgca
     1141 agctcaagag gggcgagggg acgggaacca cgatcagctt caggcccgac ccggaggtct
     1201 tcacggagac cgtcgagctc tccttcgaca ccttgagccg ccgtttcagg gagtcggctt
     1261 tcctcaacaa ggggttgaag atccggctcg tcgacgagcg cgaggagggc cgggaggtcg
     1321 cctaccagta cgagggtggc atcagggact tcgtggcgca catcaacgag gccaaggacc
     1381 ccgtgcacaa gaccgtcttc tacttccagc gcgaggacga gggggtgggg aacgtcgagg
     1441 tcgcgatgca gtggaacaac ggctttcagg actccgtctt cacctttgcc aacaacatca
     1501 acacgcacga gggcggggcg cacctctcgg gcttcagggc tgcgctctcg aggactataa
     1561 acgcttacgc gcgcgggaag gggctcctca aagagaagga ggagaacctc accggcgacg
     1621 acataaggga ggggctcgcg gccgtcatct cggtcaagat gagggacccg cagttcgagg
     1681 ggcagaccaa gaccaagctc gggaacaccg agatcaaggg cctcgtggag agctccacga
     1741 accgcttcct cgccgagttc ctcgaggagc gtcccgccga ggcgaaggcg atcatcaaca
     1801 aggcgctgca ggccgcgagg gcgcgccagg ccgcgaggaa ggtgcgggac actatccgca
     1861 aggggtatct ggagagctcg acgctgccgg gcaagctggc ggattgctct tcgaaggacc
     1921 ctgcgaggag cgagctgtac atcgtcgagg gcgactcggc cggtggtagc gcgaagcagg
     1981 gcagggagcg gaacttccag gcgatactgc ccttgcgggg caagatcctc aacgtcgaga
     2041 aggccaacat caacaaggtc ctctccaacg tcgagataca ggccatgatc agcgctatag
     2101 gcgccggggt gggggagacc ttcgacctgg ggcaggcccg ctacaacaag gtcatcataa
     2161 tgaccgacgc cgacgtggac gggagtcata tccgtacgct ggtgctgacc ttcctgtacc
     2221 ggaacatgaa ggagctgatc gaggccggtt acgtgtacat agcgcagccg ccgctctaca
     2281 agatcacgca ccagcgcaag gactactacg tctacaacga ccgggagctg caggagacgc
     2341 tgacgcgcct gagcgccaac ggcaacgcca gcatcggtcg cttcaagggc ctcggggaga
     2401 tgaacccgaa ccagctgtgg gagacgacga tggacccgca gaacaggacg ctcttgcagg
     2461 tcacggtcga gtcggcggct gtcgccgacg agctcttcac ggacctcatg ggcgacaagg
     2521 tcgagccgcg aaagcgcttc atcgaggaga acgcccgcaa ggtcaagaac ctggatgcgt
     2581 agcctggatg gacggtttcg aggtcgtcga taccgatcaa atgtccggcg caagaggcgg
     2641 gaggaatacc tgactatcaa gaacgacatt acgagggaag aagatgcttg atacgttttc
     2701 ggggaacata cgtccccaca acatagaaga cgagataaag gactcgttcc tctcctacgc
     2761 gatgagcgtg atcgtctcgc gcgccctgcc ggacgtgcgc gacggcctca agccggtcca
     2821 ccggcgcgtg ctctacgcta tgcacgacct cggcctccag ccgaaccggc cttaccgcaa
     2881 gagcgcgacg gtcgtcggcg aggtcatcgg caagtaccat ccgcacggcg actcggccgt
     2941 ctacgacact ctaaccaggc tggcgcagga cttctcgatg cgctacccgc ttgtggatgg
     3001 tcaggggaac ttcgggagcg tggacggcga cccgccggcg gcgatgaggt acacggaagc
     3061 gcgcctgatg cggatggcga ccgagatgct cagggacata agctccgaca cggtggactt
     3121 cgtccccaac ttcgacgaga gccagcgcca gcccgaggta ttgcccgcga ggttcccgaa
     3181 cctgctcgtc aacggctccg acggcatcgc tgtcgggatg gcgaccaaga tcccgccgca
     3241 caacctcggc gaggtcgtgg acgcgaccgt ggcgctcatc gacgaccccg acctcgacga
     3301 caagaacatc gccaggcaca tcaaggggcc ggacttcccg acgggaggtg tcatcgtcgg
     3361 tttgcgcggc atcagggacg ccatcgagac cgggcgcggc tccgtcaggg tgcaggcaaa
     3421 ggcgcatacc gagcagataa aggggaaccg gacccagatc gtggtaaccg agatccccta
     3481 ccaggtcaac aagagctacc tcctgcagaa gatagccgag ctggtcaagg agcgaaagct
     3541 caccgacatc tccgacctgc gcgacgagtc cgaccgcaac ggtatgcgca tcgtcatcga
     3601 gctcaagcgc gaggcggtgc cgaaggtcgt cctcaacaac ctctacaagc acacccagat
     3661 gcagcagagc ttcggggtga acctggtggc gctcgtggac ggcgttccca ggacgctctc
     3721 ctacagggag gtcctcaagc actacgtagc ccaccagttc gatgtggtca cccgcaggac
     3781 gcgctacgag ctcgagcgtg ctcgtgcaag ggcgcacatc ctcgaagggc tgatcatagc
     3841 cctcggcaac ctggacgagg tagtggcgat catccgcaag agccgctccg ttgagaccgc
     3901 gcgcaagaac ctgatcaagg ccttcaagct ctccgagcgc caggcgcagg ctatcctcga
     3961 cctcaggctg cagcgcctga cggctctgga gaggcagaag gtcgagcagg agcacaggga
     4021 cctcgtcgag aagatcgagt acctcgagag cgtgctggcc gacgaggcca aggtctacga
     4081 gatcatcaag gaggagctcc tcgaggtaag ggccgcctac gccgacgaaa ggcgcaccca
     4141 gatcaccgcc gaggagggcg ctgacttcga gattgaggac ctcatcgccg aagaagagat
     4201 ggtgatctcc atctccaacg gcggctacct caagagcgtg cccgtcaaca cgttccgcaa
     4261 gcagggccgc ggcggtgttg gcgtcgccgg gatggagctc aaagagggcg actacatcga
     4321 gcacctcttc atcacgacga cccaccacta catgctcttc ttcaccaaca agggtaaggt
     4381 gtacaggctc aaggtccacc agctcccgcg catgggccgc accgcgaagg gcaggcacgt
     4441 agccaacctc ctgccgctcg cccaggggga gacgatagcg gcggtcatcc agaccaagga
     4501 gttcaaggag gcccagtacc tcctcttcgc cacccgcaag ggtatagtga agaagacggc
     4561 ctttatggag tacaacacgc ccttgaagaa cgaggggatc atcgccatca acctggccga
     4621 cgacgacgag ctgatagacg tcaggcacgc ctcggacggc gcgagcgtcg tcatcgtgac
     4681 ccggggcggc atgggcatcc gcttctcgca ggacgaggcc aggccgatgg gacgggcaac
     4741 cgcgggggtc aaggggatca cgctcaagaa gggcgacgac gtgctgtcca tggacgtggt
     4801 ctccgaggcc ggcggggcga cggcagacct gttcatgatc accgagcaag gctacggcaa
     4861 gcgcaccgcg ctctcggagt tccgctccca ggggcgtggc ggtcagggcg tcatagcgat
     4921 gaagacgagc agagataggg gcaggctcgc gggcgtgcgg gtcgtgaggc cggacctgca
     4981 cgagctggtc atcgtctcca acgtgggcac cacgatccgg atagacgccg agtccgtctc
     5041 tcgccagggc cgcgccgccc agggcgtgag ggtgatgaac ctgcgcgccg gcgacggggt
     5101 gagcgccctc gccaaggtag tctcttcgcg cgggaccgac gccgaaggcg ctacagacga
     5161 gctctcgctc gacgagctcg tgaacgagcc cgcggcaacg gacggggcct ctccgaacgg
     5221 cggggtgagg cgtggataag ccctgtgacg gcgtggtcga gtgcacggcg ctagtcctcg
     5281 ccgagagttg cgtggagttc gagggccggc tcaccctctt cggggtgctc gacgagatac
     5341 ggcccgcgga ggaaggcgag acctccttcg tcgtctacgc caagttcgag ggctgcgagc
     5401 acgcctcgga cagggagcgg cgcgccagca tcctgatcac cgacgaggac ggcgaggtgc
     5461 ttatggagtc ggacgactac tcgatctggc tcgaaggcgc cgaggagccg gttgccatag
     5521 tcgccgcctt cgacctgccg gaggacttcg gcgacgagga acgcgtcttg tggatagagg
     5581 ccgtcctcga cgacgacatc ctctcgcaga ccgtcgtccg gctccacgac cgctacagtg
     5641 tgtaagagtt acggtagacc gggtagagac acaatgtctc tacccggcct cttttctaac
     5701 gtgaggaaat cgatccatga ctgaagatct ggcagttttc atagactggg agaacatcta
     5761 catctccacg gtcagcgagt acaactccaa gcccaacgtc tcggccatcc tggagaaggc
     5821 ccgcgagtac gggcgcatcg tctcggcgac ggcctacgcg gactggaccg acggggactt
     5881 ccgcgacgcg ccgccgaccc tctactctaa cggcatctcg ccacgctaca tctcggcgcg
     5941 ctacttcccc ggcgggaggt cccagaagcg ccgcaccaac tccatagacg tgatgcttgc
     6001 ggtggagtgc gcggacttcc tgcacaccca cccgcaagtg gacacctacg tgctggtgac
     6061 cggcgacgga gacttcatcc cgctcgtcag cctcctcagg agccggggca agaccgtggt
     6121 cgtgctcggg gtctccgagg cgacgtcgta ccacctgatc gaatccgcag accactttat
     6181 ctcctacgcc tccctcctcg aggaggagcg cgcggcgcgg gcgcgagata gacagccgaa
     6241 gaagaaggcg gccgacccct acaaggagct cgtccgggcg gtcgaggcgc taaagaaggg
     6301 cgacaagacg cgggtcctcg gccaggtgaa gcagcagatg atcgtgcagc tcggctcgtt
     6361 cgacgagcgc aacgtgggct tcaagaagtt caaggacttc gtcgtcgagg ccgagcgccg
     6421 cggcctggtc aacaccgttg accaggactt agagctccag gtctacctcc cggacgagtc
     6481 ggtccccgat ctcaccgggt ccgacctcgc cgccgaggag ccgaaaccgg acggggagag
     6541 ggccgaagcc cgcgccaacg gccggcgcga cgccgagccg cccgaggatg ggcatggcct
     6601 cgacctcctc gaggacctga cgccctacga gctgaggacc atcacgcgca gcgtggcctc
     6661 tctgaggcag cccgcttcgt tcgtcgagat cttcggcagc atccgcgacc tcgacgagcg
     6721 cggcgagctg gagctctccc gcgaggagat cgaagaggtc ataggcgcca tgctcgacgc
     6781 cgactacctg agccgggtgc gcgagaagcc ttacaaggaa gcgaaggcag acatgacttt
     6841 ctacgctctc aaccgcgacc gcgaagaggt aaagaacgcc ctcaacggca ggtagagtgc
     6901 ggcgcgggtc agtctgccag cttgtagacc gtgagcgccg caagggcgag cgcggcggag
     6961 atctggacga gcgagaggcc gacggtctcg gctacctgcg ccgccgagac gctacggaac
     7021 atcaactgca cggcgatcac ggcgccaaag ccgagaacga gcatgaggac ggccttcggc
     7081 ctgtatcccg gcccgcgctc gctccagatc gccaccccga gcagcgctgg tacgtagaac
     7141 gcaaggatca gggggatcag ccgcaagacc tcgaccccga tctgtaagag gatctccatg
     7201 gtcgctagtc tacaccagtg gttacaggtt gctggaaaat tgtcatgcaa atatgtaatc
     7261 tacttacatt gaatgttctg taaaggcggt gggcgctaat gggcataaat cagagggtct
     7321 cactcaggaa gttcgtcgac gacatccgtc gctgggtgga ggaggggagg agcgacgagt
     7381 ggatcgccag cgccctcggg accagccctt ctagcgtaca gtccttccgt tcgcgcaacg
     7441 ccatctaccg ccgcggggcc ggctccacgc tgcaccaccc ggaggactac gcctcctacg
     7501 agggcgcgat cgatgaggaa ggtcctggcg tctggttcga ccccgccgtg ggggacgacc
     7561 cgcgctggca gaagcgctgg ggagaaacgg agagggtcga gttgcgcctc accccgacca
     7621 ggatcgttct cctgaggcgc agcggctccc gtgcgcgccg cctgcagcct agcgcctgac
     7681 caactgcggt ataattaatg cagggtctgg gccgtccgac ccgtcgctgt gtcccggaat
     7741 tttccaggat atagagcaaa gtccggactc gtagcaaagg ccgctcgggc caataacccg
     7801 aggggcggcg acggcgcaag cccgccgcca cgtcaagtgc aacagagagc agaccagccg
     7861 atggcctgta gagatgcaac atgttgcgtc tctacacggc acaggtgatg ggtgaaaggg
     7921 tggggtaaga gcccaccggc ggtatcccgc gagggaccgg ccaggtaaac gaccggtgcg
     7981 agcaagccaa aatgtcccgt aggggcgttt cgcgaaacac ccctacgggc tggtaggcgc
     8041 ggctcctcaa agagcccggc gtagcaacgt gttaac
//
LOCUS       DASTUV010000180         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1401858, whole genome shotgun sequence.
ACCESSION   DASTUV010000180 DASTUV010000000
VERSION     DASTUV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401858"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFT03_03695"
     CDS             <1..1107
                     /locus_tag="VFT03_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEU4847276.1"
                     /translation="DRRFEELVGAIARGRDFSLEEVRSAIDGAPYSAPEGLSRGLLDG
                     VCYEDELPESLGAEGSRAKLVEWSAARRSLRVPYRRSPRRRVGLLSLSGTIVRGKSRR
                     LPVPLPLLGGEQAGSESVVAALRLAEKNRRVAAILFHVESPGGDALASDLIWREVERI
                     RAKKPVVVLMGNAAASGGYYVSAAATHVVARRSTITGSIGVLSIRPVARGFYEKIGVH
                     PGSVERGARAGLLDLSRSPTPDELRVLREQISYIYDAFKERVTRGRGLGATELEDVAG
                     GRVWTGVEALELGLADEVGGFDEALHKARDLGKVERDAPEALIKISPPRSGRPAPGKP
                     AEAAREMVDEIRDAVLDLRAVRLWAVSPYDIRED"
     gene            1148..2329
                     /locus_tag="VFT03_03700"
     CDS             1148..2329
                     /locus_tag="VFT03_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847277.1"
                     /translation="MSASPRKDVDRRAMSVLSAGHLFTDLNQGAVAALLPFLVAERGL
                     TLAAAGTLVLAATASSSVVQPLFGIFSDNRPLPALMPLGVLSAGAGMALAGVAPSYPL
                     ILLCVVLSGIGVAAFHPEAARFANYVSGSRRARGMSFFSVGGNAGFALGPALTTPLVL
                     AFGLPGTLFLALPATMMAAVLFLEIPRMKGFEPAAVESEGAQPAAPEHWGPFVRMVMI
                     VTVRSFVYFGLIAFVASYYERVLGTSTAFGNAALTAMLAAGAVGTLLMGPLADRFGRR
                     AVVGTSMLLLPPLIYGFTLSGPVLGMALLALVGAVTVGTFGVTVVMGQEYLPGRIGLA
                     AGITMGLSIGLGGLGAPVLGLLADYAGLSVTMLVIAALPVIGLFLTLTLPRNVPSSPK
                     I"
     gene            2429..>3287
                     /locus_tag="VFT03_03705"
     CDS             2429..>3287
                     /locus_tag="VFT03_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU4847278.1"
                     /translation="MRAFQLLGLFAHRFRWAILVTWAMLVIASVFFAPDLSGRLKGGG
                     FEGSNSEAERVQDLMSEEFGVSPATLIVVFEGNGLSARGEEFQRAQKEALEGVRRMPE
                     VRDVRTYAGTQDTRFISDDGEKAYAVIAFNVSIDETRNVVDEVRGKVRSEELDTYVTG
                     AAAVYQDLEEASNEDIKRAEKYAFPFALLILLLAFGTVVAAGVPVLIGGVSVLTTLAA
                     LYFVAGSYDMSVFVLTLSTMLGLGLGIDYALFFVSRFREELESYPVSEAVSRTVTTAG
                     RSIFFSGTAV"
BASE COUNT          475 a         1080 c         1104 g          628 t
ORIGIN      
        1 gaccgcaggt tcgaggagct ggtaggcgct atcgcacggg ggagagactt ctcccttgag
       61 gaggtacgct ccgccataga tggcgccccc tactccgcac ccgaagggct atcccgcggc
      121 ttgctcgacg gtgtgtgcta cgaggacgaa ctgccggaga gcctgggggc ggaagggtca
      181 cgcgcgaagc tcgtggagtg gagcgcggcg cggcgttcgc ttcgcgtacc gtaccgcagg
      241 agcccgcgcc ggcgggtagg gctcttgagc ctctcaggga ctatcgtccg ggggaagagc
      301 cgaaggttgc cggttccgct gccgctactc ggcggcgagc aggcggggag cgagtctgtc
      361 gttgccgccc tgcggctcgc ggagaagaac cggcgagtag cggctatctt gtttcatgtc
      421 gagtcacccg gcggagacgc gctggcgtct gacctgatct ggcgcgaggt agagcgcatc
      481 cgcgcgaaga agcccgtggt cgtactcatg gggaacgcag cggcctccgg tggttattac
      541 gtctcggcgg ccgcgaccca cgtcgtcgcc cgccgctcca ccataacggg gtccatcgga
      601 gtcctctcca tccgccccgt cgcccgcggc ttctacgaga agataggggt acacccgggc
      661 tccgtggagc gcggcgccag ggcaggtctc ctggacctca gccggagccc tacgccagac
      721 gagctgcgcg ttctgcgaga gcagattagc tacatctacg acgcgttcaa ggaacgcgtc
      781 acgcgcgggc gcggcttggg agctaccgag ctcgaggacg tggcgggggg gcgcgtctgg
      841 accggagtcg aggccctcga gctcggcctc gccgacgagg tgggcggctt cgacgaggcg
      901 ctgcacaagg cgcgcgatct gggcaaggtg gagcgggacg cgccagaggc cttgatcaag
      961 atctcccctc cccgcagcgg tcgtcccgcg ccgggaaagc cggcggaggc ggcccgcgag
     1021 atggtcgatg agatcaggga tgcggtcctc gatctccgcg cggtacgctt gtgggctgtc
     1081 tcaccgtacg atatacgcga ggattgatag tatcccctcg tcgggaaaca cgacagaagg
     1141 gtggtacttg agcgcgagcc ctagaaaaga cgtcgaccgg cgggcgatgt ccgttttgtc
     1201 ggccggacac ctgttcacgg accttaacca gggtgccgtg gcggcgctgc tcccgttcct
     1261 cgtggcggag cggggcctca ctttagcggc ggccgggacg ctggtgctcg ccgctaccgc
     1321 ctcctcctct gtcgtccagc cgctcttcgg gatcttctcg gacaaccgcc cgctcccggc
     1381 gctcatgccg ctcggggtgc tctcagcggg cgccgggatg gcgctcgccg gcgtcgcccc
     1441 cagctatccc ctgatcctgc tgtgtgtcgt cctgagcggt atcggggtcg ctgcctttca
     1501 ccccgaagca gcccgcttcg ctaactatgt ctccggctcg cgacgcgcgc gcgggatgag
     1561 cttcttctcc gtcggcggaa acgctggctt cgccctcggt ccggctctga ctaccccgct
     1621 cgttcttgcc ttcggtctgc ccggcacgct gttcctcgcg ttgcccgcca cgatgatggc
     1681 tgccgtgctc ttcctcgaga tcccgcgcat gaagggattc gagcccgccg cggtcgagag
     1741 cgagggtgcg cagccggcgg cgcctgaaca ctgggggcca ttcgtacgta tggtcatgat
     1801 cgtcacggtg cgctccttcg tctacttcgg gctcatcgcc ttcgtggcct cttactacga
     1861 gcgcgtgctc gggacgtcga cagccttcgg caacgccgcc cttaccgcga tgctcgctgc
     1921 tggagcggtg gggacccttc tcatgggacc gctcgcggac aggttcgggc gccgcgcggt
     1981 agtcggcacg tccatgctcc tgctgccccc actcatctac ggcttcacgc tctcggggcc
     2041 ggtcctgggg atggccctgc tcgcgctggt cggcgcggtc accgtgggca ctttcggcgt
     2101 tacggtagtg atgggacagg agtatctgcc cggccgcata gggctcgccg ccggcatcac
     2161 gatgggactc tcgatcggcc tcggcgggct cggcgccccc gtcctcgggt tgctcgccga
     2221 ctacgccggc ctctcggtca cgatgctggt catagctgct ttgcctgtaa tcgggctgtt
     2281 cctcactctg acactgcccc gcaacgtccc gtcttctccc aaaatctgag atctaccagc
     2341 cctcctgtga tagaagcctc agtcacctcc tgatggtcgg gtaccatact ctggatgaat
     2401 tcagctcctg cacgcacgct ccgctgagtt gagggctttc cagcttctcg gcctgttcgc
     2461 ccaccggttt cggtgggcca tcctcgtgac gtgggccatg cttgtgatcg cgagcgtgtt
     2521 cttcgctccg gatctctcgg gccgtctcaa gggcggaggc ttcgagggct cgaactcgga
     2581 agccgagagg gtgcaagatc tcatgtccga ggagttcggt gtctctccgg cgaccctcat
     2641 tgtggtcttc gaagggaacg ggctctccgc ccgcggcgag gagttccagc gcgcgcagaa
     2701 agaggccctc gaaggggtgc gccggatgcc cgaggtccgc gacgtcagaa cctacgccgg
     2761 tacgcaggac acgcgcttca tctccgacga tggagagaag gcctacgccg tgatcgcctt
     2821 caacgtctcc atagacgaga cgcgcaacgt ggtggacgag gtgcgcggca aggtgcgctc
     2881 cgaggagctc gatacctacg tcacgggtgc ggcggccgtc tatcaggacc tggaggaggc
     2941 gagcaacgag gacataaagc gcgccgagaa gtacgctttc ccgttcgcgt tgctgatctt
     3001 gctactcgcc ttcggtacgg tcgtcgctgc cggagtaccg gtgctcatag ggggggtgag
     3061 cgtactgacc accctcgccg cgctgtactt cgtcgctggt tcctacgaca tgtccgtctt
     3121 cgtgctgacc ctctccacta tgctcgggct tggcctcggg atagactacg cgctcttctt
     3181 cgtcagccgc ttcagggagg agctcgagag ctaccccgtc tccgaggcgg tctcgaggac
     3241 ggtgacgacg gccgggaggt ctatcttctt ctcgggcacg gcggtcc
//
LOCUS       DASTUV010000181         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_730179, whole genome shotgun sequence.
ACCESSION   DASTUV010000181 DASTUV010000000
VERSION     DASTUV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730179"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFT03_03710"
     CDS             <1..809
                     /locus_tag="VFT03_03710"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU4847279.1"
                     /translation="DGPVVVSGNIAADAGKQLRVAAVDDPAAFARTALIEALGRAGVS
                     VSASPTGSNPSSELPKKGSYKAEDQVAVYESPPYSQYAKLILKVSHNYGANLNVCLMA
                     VKAGSTDCNDGFPAMKSFFEKAGLDVDQVALADGRGGNPTDRFTPKAATDLLRYWLEQ
                     PQAEEFREMLPELGVNGSLASDCKDCPAKGKVFAKTGTVGLPDYVNESIIDNESLGGY
                     LEAEPGRYHVFYLVVNGASAQNIDEVLKVFNDLSDIAAILQEDASHQGGE"
     gene            1093..1797
                     /locus_tag="VFT03_03715"
     CDS             1093..1797
                     /locus_tag="VFT03_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847280.1"
                     /translation="MEPVTIVVALSAFGLYSLTIVLLYPGHYEAYLSPFYSPPVPRPE
                     WLPAFITAPMFVLWIPLGFRASCYYYRKAYFRAFFWDPPACSAGAQRREPRSPESYRG
                     ERGVFVLNNVHRYFLYASLVVVAFLWYDAVLTFFPDGSFGVKVGSIIWLVNVTLLTLY
                     TISCHSMRHLIGGYRDCYTCVRGGNASRKAYDGISKLNLRHPLWAWLSLGSLLLADVY
                     LRLIAAGVIPDLTIIG"
     gene            1809..3596
                     /locus_tag="VFT03_03720"
     CDS             1809..3596
                     /locus_tag="VFT03_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847281.1"
                     /translation="MERDEHYEVREHDVLIIGAGGAGLRAAIAAAEKGLSVGIVSKSL
                     LGKAHTVMAEGGIAAAMSNVDPDDSWRQHFIDTMNSGKFLNNWRKVEIYTKEAPEGVY
                     ELEQWGALFNRTPEGKISQRPFGGHTYRRLAHVGDRTGLEMIRTLQEKALATDAEVYM
                     ETTVTRLFKDARGRIIGALAYTRENGKFIYFKTKAVVMATGGWGRIYKVTSNSWEGTG
                     DGVILAYEAGADLVDMEMVQFHPTGMVWPPGARGILVTEGVRGEGGVLLNADGERFME
                     RYDPDKMELSTRDVVARANYTEVQEGRGTPHGGVYLDITHLGYDNIMKKLPTMYEQFL
                     KLADIDISREPMEVFPTVHYNMGGIKVEPENCATNVPGLFAAGEAAGGLHGANRLGGN
                     SLGDLLVFGRRAGTGAAEYLEQSEHGTEVEEDEIRAEIRRMLEPLEKPPGDHDESPYL
                     LQQELQEVMMEHANLMRTEESLQEGLDKILALKNRLPNIKVGGTRLFNPGWHTAQDAR
                     YLILISEIIVRCALERKERRGAQWRLDYDGPDEELGKINFVVKRGEQGEASIERHEIS
                     PMPEHLAELFEGKVADMAGGERRGAESQF"
     gene            3617..>4099
                     /locus_tag="VFT03_03725"
     CDS             3617..>4099
                     /locus_tag="VFT03_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4847282.1"
                     /translation="MDEKAAREKDMASKAHADVLADEKPLDGRTAKLRIFRGDAEGGE
                     EVEYEIPLVEGMVVLDAVLYVQAHEAGDLAVRWNCKAAHCGSCSAEINGRPKLLCKTR
                     VEEYEGAEIRVGPLRAFPVIKDLVSDVSWNYEVSRGITPFTTRQQAPFTMYEEDVERL
                     Y"
BASE COUNT          818 a         1246 c         1353 g          682 t
ORIGIN      
        1 cagatgggcc ggtggtggtc agcggaaata tcgccgctga cgcaggcaag caactgcggg
       61 tcgcggcggt cgacgacccg gccgcctttg cgcgcacggc gctcatagag gcgcttgggc
      121 gcgctggcgt ctccgtgagc gcctcgccca caggctcgaa cccctcctcc gagctgccca
      181 agaagggctc ctacaaggcg gaagatcagg tcgcggttta cgagtctccg ccctacagcc
      241 agtacgccaa gctgatcctc aaggtcagcc acaactacgg cgccaacctc aacgtatgcc
      301 tgatggccgt caaggcgggc agcaccgact gcaacgacgg cttccctgcg atgaagtcct
      361 tcttcgagaa ggcgggcctt gatgtggatc aggtggccct ggccgacggc agggggggta
      421 accccacgga ccgcttcaca cccaaggcgg cgaccgacct gcttcgctac tggctggagc
      481 agcctcaggc ggaggagttc cgcgagatgc tccccgagct gggtgtcaac ggcagcctcg
      541 cgtcagactg caaggactgc cccgcgaagg gcaaggtctt cgccaagacc gggacggtcg
      601 ggctccccga ctacgtgaac gagagcatca tcgacaatga gtcgttgggg ggctacctgg
      661 aagcggaacc cggccgctac cacgtcttct acctcgtggt caacggcgcc tcggcgcaga
      721 acatcgacga ggtcctcaag gtgttcaacg acctctccga catcgccgct attttgcagg
      781 aagacgcctc acaccaggga ggggaatagc agatcgacgc aaacgtccac ccccgccggt
      841 caggacgata atttccccat tggaccccgc agcgttcgtc atctgctcta ggtcgagctg
      901 gcgaaggcgc gcggggtctg cgtctacgtc tcgacgctct gattatcata atcgtttcac
      961 agaacctccc ggagcgatgc tctatacttg cgaggccaag caaggagcca tagttacaag
     1021 aagttacaag taactagagg gagtaggagt ttgtctagca tagccgccgc ttggaagagg
     1081 ttaccctggt gggtggagcc ggtaaccatc gtggttgcgt tgagcgcctt tggcctgtat
     1141 tcgcttacga tcgtgctcct gtatcccggt cattacgagg cgtacctatc gccgttctat
     1201 tcgccgccgg tcccgaggcc cgagtggttg ccggcgttca tcacggctcc gatgttcgtt
     1261 ctgtggatac ctctaggttt cagggcctcg tgctactact accgcaaggc gtacttcagg
     1321 gcgttcttct gggacccgcc ggcctgctcc gcgggggcgc agcggcgtga gccgaggagc
     1381 cccgagagct accggggtga gcggggtgtc ttcgtcctga acaacgtgca caggtacttt
     1441 ttgtatgcct cgctcgtggt cgtggccttc ctctggtacg acgcggtcct gaccttcttc
     1501 ccggacgggt cgttcggggt gaaggtaggc tccataatct ggctggtaaa cgtcacgctg
     1561 ctcacgctct acaccatctc gtgccattcg atgcggcacc tgatcggagg ctacagggac
     1621 tgctacacgt gcgtcagggg cggcaacgcc agtcgcaagg cgtacgacgg catcagcaag
     1681 ctcaacctca ggcacccgct ctgggcctgg ctcagcctgg gctcgttgct gttggcggac
     1741 gtatacttga ggctcatcgc ggccggggtc atacccgacc tgacgatcat aggataggta
     1801 ggtagaaggt ggagagagac gagcactacg aggtgcgcga gcacgacgtt ctgattatcg
     1861 gcgccggcgg cgcgggcttg agggctgcca tcgccgcggc ggagaagggc ctctcggtcg
     1921 ggatagtctc gaagagcctg ctcggcaagg ctcacaccgt tatggccgag ggcggcatcg
     1981 ccgctgccat gtccaacgtg gaccctgacg actcctggcg ccagcacttc atagacacga
     2041 tgaacagcgg caagttcttg aacaactggc gcaaggtcga gatctacacc aaggaagccc
     2101 ctgagggggt ctacgagttg gagcagtggg gcgccctctt caaccgcacg cctgagggca
     2161 agatctccca gagacccttc ggcgggcaca cgtatcgcag gctggcccac gtgggagacc
     2221 gcacgggtct ggagatgatc cggacgctcc aggagaaggc cctcgctacg gacgcggagg
     2281 tctacatgga gaccaccgta accaggctgt tcaaagacgc tcggggccgc atcatcggtg
     2341 ccctggccta cacccgtgag aacggcaagt tcatctactt caagacgaag gccgtcgtca
     2401 tggcgaccgg cgggtgggga cgcatctaca aggtcacctc caactcctgg gagggcaccg
     2461 gcgacggggt catcctggcg tacgaggccg gggcggacct tgtggacatg gagatggtgc
     2521 agttccaccc cactggcatg gtctggcctc ccggagcccg cggcatactg gtcaccgagg
     2581 gcgtacgcgg cgagggcggt gtcctcctta acgccgacgg cgagcgcttt atggagagat
     2641 acgatcccga caagatggag ctctccaccc gcgacgtcgt cgcgagggcc aactacaccg
     2701 aggtgcaaga aggccgggga acgccgcacg gcggggtgta cctggacatc acgcacctgg
     2761 gctacgacaa catcatgaag aaactgccca ccatgtacga gcaattcctc aagctcgccg
     2821 acatagacat ctccagggag ccaatggagg tcttccccac cgtccactac aacatgggcg
     2881 ggatcaaggt cgagccagag aactgcgcga ccaacgtgcc tggactgttc gccgccggcg
     2941 aggcggccgg cgggctgcac ggggcgaaca ggctcggcgg caactcgctc ggcgacttgc
     3001 tggtgttcgg gaggcgtgcc gggacgggcg cagcggagta tctggagcag agcgagcacg
     3061 ggaccgaggt cgaagaagat gagatacggg ccgagatccg ccgcatgctc gagccgctgg
     3121 agaagccccc cggtgaccac gacgagagcc cttacctcct gcagcaggag ctccaggagg
     3181 tcatgatgga gcacgcgaac ctcatgcgta ccgaggaatc cctccaggaa ggcctggaca
     3241 agatcctcgc gctcaagaac aggttgccga acatcaaggt gggggggacg cgtctgttca
     3301 accccggctg gcacactgcg caggacgcgc ggtacttgat cctgatctcc gagatcatcg
     3361 tccggtgcgc ccttgagcgt aaggagcgtc gcggggcgca gtggcgcctc gactacgacg
     3421 ggccggacga ggaactcggc aagatcaact tcgtcgtaaa gaggggcgag cagggagaag
     3481 cgagcatcga gaggcacgag atatccccga tgcccgagca cctggccgag ctgttcgagg
     3541 gcaaggtcgc tgacatggct ggcggagagc ggcgcggcgc agagagccag ttctaggata
     3601 ggagagcgtt acgcacatgg acgagaaggc cgccagagag aaggacatgg cctccaaggc
     3661 gcacgccgac gtgctcgccg acgagaagcc ccttgatggc aggaccgcga agctcaggat
     3721 cttcagggga gatgcagaag gcggcgagga ggtcgagtac gagatcccgc tcgtcgaggg
     3781 gatggtcgtc ctcgatgcgg tcctctacgt ccaggcgcac gaggccggtg acctggcggt
     3841 gcgctggaac tgcaaggctg cccactgcgg gtcgtgcagc gcggagatca acggccgccc
     3901 caagctgctg tgcaagacaa gagtggagga atacgaggga gcggagatac gggtaggccc
     3961 cttgagggcc ttcccggtca taaaggacct cgtctccgac gtctcctgga actacgaggt
     4021 ctccaggggg atcacgccat tcaccacccg ccagcaggcg cccttcacga tgtacgagga
     4081 ggacgtcgag cgcctctac
//
LOCUS       DASTUV010000182         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1473319, whole genome shotgun sequence.
ACCESSION   DASTUV010000182 DASTUV010000000
VERSION     DASTUV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473319"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..589)
                     /locus_tag="VFT03_03730"
     CDS             complement(185..589)
                     /locus_tag="VFT03_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4847283.1"
                     /translation="MSESQPKTVEQMVAEAEACIESLSPEQVAAEMGRTNITLVDVRE
                     DDERFLEGAIPGSVHVPRGMLELSADPDSPLHRQELGRDHRIILYCSSGTRSALSAAT
                     LNVMGYENVAHLSGGMMAWKRGGQPVEQIPFA"
     gene            complement(819..1298)
                     /locus_tag="VFT03_03735"
     CDS             complement(819..1298)
                     /locus_tag="VFT03_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4847284.1"
                     /translation="MGLTPFRGFYDMQSEMNRMLDEVFGSLPRAGGRQRGEAPSQWAP
                     TLDVLQEDGDIVVRAELPGVKQEDVDITFHNGVLTISGERRAEEQKEGAGYYVRERRY
                     GSFRRSMSLPQGTDESSISARFEDGVLEVRVAGAAAVQEPRRIQIEGSEGRSEEETG"
     gene            1448..1978
                     /locus_tag="VFT03_03740"
     CDS             1448..1978
                     /locus_tag="VFT03_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4847285.1"
                     /translation="MRERMSGAERGLVGRRLVAIRERRMWTQGRLAEEAGLSPTTVSG
                     IESGRISRPHFGTLRKLARALGVESEELVSGLGSDGESVASPLSLDWAMSSDEDEFER
                     GLEGASVEGLSSLSRELDEEEGRLRRMYGEARGSEQRRLIKTMIRQVAANSGSVEASI
                     TAHEDNRAPDRPSPGE"
BASE COUNT          337 a          664 c          623 g          389 t
ORIGIN      
        1 gccctcgcgc gcgtcgagga gcccgaggag ctctcagaac aaggtggctt cacgggcgcc
       61 ccggacatga gtcacgaccc gcgtcccgag gagcgagagg gctagttcct gctccgtcga
      121 acggtcctcg taacgttctg gagtaagtcc ttctgtagag tcgtccaaac cgaggagtag
      181 acactcaagc gaaaggtatc tgctctacgg gctgtccgcc ccgcttccac gccatcatcc
      241 ccccgctaag gtgggcgacg ttctcgtatc ccatcacgtt gagagttgcc gcgctcagcg
      301 ccgagcgagt ccccgacgag caatagagga tgatcctgtg gtctcgaccc aactcctgcc
      361 gatggagggg gctgtcgggg tccgccgaaa gctccagcat ccccctgggc acgtgcaccg
      421 agcccgggat ggcgccttcc agaaatcgct cgtcgtcctc gcgcacatcg acgagggtaa
      481 tgtttgttct ccccatctcg gcggccacct gttctggcga gaggctctcg atgcacgcct
      541 cggcttcggc gaccatctgt tcgacggtct tcggttgcga ctcggacaag atcacacgcc
      601 ctccatgtcg tgagcaggcc gttcgaggac catgcggcgc ggttcgcacg ctgaactgtc
      661 ctagcggccg gcttctttca attgccgttc cggctcggcg acgcgctcgt tcctcgcccg
      721 gctcttccgc cgccagtcta gcgcgaaaga tcgggctaga gacgtagcag cgcgctacgt
      781 ctctagcccg tctcctcctc gggccggcct ctacgtcttt agcccgtctc ctcctcggac
      841 cggccttccg agccctcgat ctggatgcgc ctgggctcct gtaccgcggc cgcacccgcg
      901 acccttacct cgagcacacc gtcctcgaac cgcgcgctga tgctgctctc gtccgtaccc
      961 tgcggcaacg acatgctcct ccggaacgat ccgtaccggc gctccctcac gtagtagccg
     1021 gctccttcct tctgctcctc agcccggcgt tccccgctga tggtcagaac cccgttgtgg
     1081 aacgtgatgt ccacatcttc ctgcttcaca ccaggcagct cggcccgcac cacgatgtcg
     1141 ccgtcctcct ggagcacgtc gagcgtcggc gcccactgtg aaggcgcctc acctcgctgg
     1201 cgtccccccg cccggggcag ggagccgaat acctcgtcga gcatacggtt catctcactc
     1261 tgcatgtcgt agaaaccacg gaacggagtc aagcccatct cgaacacctc cttctcctcg
     1321 cgccctaccg gcgctaacct ctgcctgcgc ccgcattata cccctctact acagcttgta
     1381 aacagttttc agacagtttt tccgtgattt attacagttt ttgctcgatt tatggtagag
     1441 tacggccatg agagagagga tgtctggcgc ggagaggggt ctggttggca ggaggctggt
     1501 agccatccgc gagcggcgga tgtggacgca ggggcggctg gccgaggaag cggggctaag
     1561 cccgaccact gtgtcgggga tcgagagtgg caggatctcg cgcccccact ttggaacgtt
     1621 gcgcaagctg gcgagagctc tgggtgtgga gtctgaggag ctcgtgtcgg ggctgggatc
     1681 ggacggagag agcgtggctt ctcccctgtc actcgactgg gcgatgtctt cggacgagga
     1741 cgagttcgag cgggggctag agggtgcttc ggtggaaggc ttgagctccc tgtcgcggga
     1801 gctcgacgaa gaggaggggc gtttaaggcg gatgtatggt gaggccaggg ggagcgagca
     1861 gcgcaggctc ataaagacga tgatccgcca ggtcgccgcc aactccggct ccgtggaagc
     1921 ctcgatcacg gcgcacgaag acaacagggc cccggaccgg ccttcgcctg gcgagtagga
     1981 acggcgtccc caccgacggg ctaacgattc ggg
//
LOCUS       DASTUV010000183         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_38373, whole genome shotgun sequence.
ACCESSION   DASTUV010000183 DASTUV010000000
VERSION     DASTUV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38373"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VFT03_03745"
     CDS             complement(<1..1484)
                     /locus_tag="VFT03_03745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU4847286.1"
                     /translation="MASRVSQDIGDYPFDLIVVGAGINGAGIARDAAMRGLRVLLLDK
                     GDIASGTTQWATRLIHGGLRYLEYYEVSLVRESLRDREILLHIAPHLVKPLGFLVPIY
                     ERAKHGPLMVRLGMFGYDTLSLDKSLPNHSMLSAEEALEREPGLNPEGLLGAAFYYDA
                     QVEYAERIAVENAISAHEHGAVVLTYARVDRLVREGDSVTGVEFTDVLGGGTYQAHAP
                     VTVNVAGPWVDEVLGDVSEGRMIGGTKGSHLIVDPFPGAPKNEALYVEARRDGRPYFI
                     VPWNGRYLIGTTDIRYDGDLDRVVADEEEIDYLIEETNYVIPEANLTRESVLFTYSGI
                     RPLPFQPEGAEGSVTRSHIVYDHARGRGIGTKRSSVGEDAPRVEGLISIVGGKLTTYR
                     NLSRQTVDMVYEKTGREAPESRTSKVPLPGGETDDFEAFAARFKVTSGLTDWLSERLL
                     RLYGVRALDVLEVAGEDPSLRMPLGPSASVESGIIGAEVLYALRR"
     gene            complement(1496..1891)
                     /gene="dhaM"
                     /locus_tag="VFT03_03750"
     CDS             complement(1496..1891)
                     /gene="dhaM"
                     /locus_tag="VFT03_03750"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEU4847287.1"
                     /translation="MVGLVLVSHSPQIAEGTAELVQQMAGEVALTAVGGDAGGGFGTD
                     PERIKTAIEDLDADEVLVFMDLGSAVLSAETVLEMLSSEQREKVRLVDAPFVEGAFAA
                     GVVASAGSDADECVEAAMEARTESKLHAE"
     gene            complement(1891..>2392)
                     /gene="dhaL"
                     /locus_tag="VFT03_03755"
     CDS             complement(1891..>2392)
                     /gene="dhaL"
                     /locus_tag="VFT03_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEU4847288.1"
                     /translation="AALERLDGAAPGSPADALKAVSMALISKVGGAAGPLYGTAFLRA
                     STALQGKDEVSAEDAAEALEAALGGIKQRGKAEVGAKTIVDALTPAAEAAKEAAGEPD
                     ASVAGVFRAAAEAAKEGAESTVPLTARRGRASYLGPRAAGHQDPGATSTYYLLDAAAR
                     SLEESS"
BASE COUNT          357 a          852 c          764 g          419 t
ORIGIN      
        1 cgcctgagcg cgtagagcac ctcggcgccg atgatgccgc tctcgacgct agcggaggga
       61 cctagaggca tccgcagcga cgggtcttcg cctgctacct cgagcacatc cagcgcgcga
      121 acgccgtaga gcctgaggag cctctcggag agccagtctg tcaggccact cgtcaccttg
      181 aagcgcgcgg cgaaggcttc gaagtcatcc gtctctcccc ccggcaacgg caccttcgag
      241 gtgcggcttt ccggcgcctc gcggccggtc ttctcgtaga ccatatccac cgtctggcgg
      301 gagaggttgc ggtaggtcgt aagcttgccg ccgacgatgg agatcaaacc ctcgacccgc
      361 ggagcgtcct cccctacgct gctccgcttc gtcccgatgc ccctgcccct agcgtggtcg
      421 tagacgatgt ggctccttgt gacggatcct tcggcgcctt cgggctggaa tggtaggggc
      481 ctgataccgg agtaggtaaa cagcacgctc tcgcgcgtga ggttcgcctc ggggatcacg
      541 tagttcgtct cttctatcag gtagtcgatc tcctcctcgt ccgccacgac gcggtcgagg
      601 tcaccgtcat agcggatgtc ggtggtcccg atcaggtacc gcccgttcca cggcacgatg
      661 aagtagggtc gcccgtccct gcgcgcctcg acgtagaggg cctcgttctt cggagctccg
      721 gggaaggggt caacgatcag gtggctgccc ttggtccccc cgatcatgcg cccttcggag
      781 acgtccccga gcacctcgtc cacccaagga ccggctacgt tgacggtgac gggggcgtgc
      841 gcctgatacg ttccgccgcc gagcacgtcg gtgaactcga ccccggtcac gctatctccc
      901 tctcggacga gccggtcgac cctggcgtaa gtcagcacga cagcaccgtg ctcgtgcgcg
      961 gagatcgcat tctcgacggc tatcctctcg gcgtactcga cctgggcgtc gtagtagaag
     1021 gccgcgccga gcagaccctc cgggttgagg cctggctcgc gctccagcgc ctcctccgcg
     1081 ctcaacatgc tgtggttggg caagctcttg tcgagggaga gggtgtcgta gccgaacatg
     1141 ccgagcctta ccatgagcgg cccgtgcttg gcgcgctcgt agatcgggac gaggaagccc
     1201 agaggcttga cgaggtgagg agctatgtgg aggagtatct ccctgtccct cagggactcc
     1261 cgcaccagcg acacctcata gtactccagg tagcgcaggc cgccgtggat caggcgcgtc
     1321 gcccactgcg tcgtgccgct ggcgatgtcc cccttgtcca gaaggagcac cctgaggccg
     1381 cgcatcgccg cgtccctggc gatccctgct ccgtttatcc cggccccgac gacgatgagg
     1441 tcgaacgggt agtccccgat gtcctgcgac actctgctgg ccataacgtc tccttctatt
     1501 ccgcgtggag cttcgactcg gtgcgggcct ccatcgccgc ctcgacacac tcgtcagcgt
     1561 cagagccggc agaggccacg accccggcgg cgaaggcgcc ctcgacgaac ggcgcgtcca
     1621 ccaggcggac cttctctctt tgctcggagg atagcatctc gagtaccgtc tcggcggaga
     1681 gaacggccga ccccagatcc atgaagacga gcacctcgtc ggcgtccaga tcctctattg
     1741 cagtctttat cctctcgggg tcggtcccga agccgccgcc tgcgtctccg ccgacggccg
     1801 tcagcgcgac ctcacccgcc atctgctgca ccagctcggc ggtaccctcg gctatctgcg
     1861 ggctgtgcga gacgagcacg aggccgacca tcaggatgac tcttccaggg agcgggccgc
     1921 cgcgtcgagc aggtagtagg tagaggtcgc acccgggtcc tggtgcccgg cggcacgagg
     1981 cccgaggtag ctcgcccggc ccctcctcgc cgtgagcggg acagtggact ccgcaccctc
     2041 cttcgcggcc tcggcagcgg ccctgaatac gccggccacg ctcgcatcag gttcgccggc
     2101 cgcctctttc gcggcctccg ccgcgggggt gagggcgtca acgatcgtct tggcccccac
     2161 ctcggctttg ccgcgctgct tgatcccccc cagcgccgcc tcgagcgcct ccgcggcgtc
     2221 ctcggccgag acctcgtcct tgccctggag cgccgtcgag gcgcgcagga acgccgtacc
     2281 gtatagcggg ccagccgcgc cgccgacctt gctgatcagg gccatcgaga cggccttcag
     2341 ggcgtcggcc ggggagccgg gggccgctcc atcgagacgc tcgagggcgg cc
//
LOCUS       DASTUV010000184         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_727021, whole genome shotgun sequence.
ACCESSION   DASTUV010000184 DASTUV010000000
VERSION     DASTUV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727021"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1120
                     /locus_tag="VFT03_03760"
     CDS             146..1120
                     /locus_tag="VFT03_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847289.1"
                     /translation="MISRLFSREPDTRIFFATDIHGSEVCWRKFLNAGKHYEADIMVL
                     GGDMTGKALVPIVEEGSGRWHASLLENRYDFETEEELTEFEDAVRRRGYYPFRTDPDE
                     MSELGSNEKRREKLFHEQMLGTIERWMRMADERLGDSDIRCLVSPGNDDQFEVDEIVA
                     AAKRVELAEGRVVEFGGFQMASTGWSNRTPWDTYREEDEDALLERLRKMTSQVTAPPE
                     RTIYNFHCPPYGSGLDEAPELTDDLRPKHGGRSIVPVGSKAVREAIEEGQPVLALHGH
                     IHEARGNTRIGRTLCINPGSSYEQGQLLGAVINLDGKGKVKRFVLTSG"
     gene            1136..2752
                     /locus_tag="VFT03_03765"
     CDS             1136..2752
                     /locus_tag="VFT03_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU4847290.1"
                     /translation="MAGNTTAGSDAGDLFQRKATGLLRGWSVWDGFLYAAFSINLITL
                     GLWIFSFGPFIPDGALIWAIILSGVYLVFQAITYASLIAVMPRAGGDYVWQSRVLGGG
                     IGFVLAVTGWWFILWHWVPIYANILNVEVLVPLATILELDSVSGFFSGPAGLFTASMV
                     VAILASVYISLGMRAYARIQKFCFYGGLVGLAIMLVLLLVNSNADFVGAFNSQASELY
                     GASGNAYQQTLEAGSIEAAGPATLALGPTLLLIPMIVFFNLWSSWGATLYGEVRGASD
                     FRKNIYAMGGALVVTSIIAVIMLALISKTFGWDFYYASANAYWSGAENAPTSVWPYPG
                     LFAAFFFDNPILQVILVGLLSLWFFGWVGTVFLSSTRVVFATAFDRVLPEAVARTSRN
                     GVPYVALALMLIPSIPVSYLYAFGENFATYTLDATLVIAITFLGSAIAAAVLPWRRPE
                     IYNASPIARYNVLGIPLITVAASAFAIFLVFCLFKWLTDATYGINNGQSLIYMVGLYV
                     VALLLYVGFRLYRSSQGMDLKMVYSEIPED"
     gene            complement(2825..>3133)
                     /locus_tag="VFT03_03770"
     CDS             complement(2825..>3133)
                     /locus_tag="VFT03_03770"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847291.1"
                     /translation="TEQAGDDPVVVDAMTFSENPVGIISAYCERVGIAFRPDALSWEP
                     GEVEKWKSWEGWHEAAQQSTGIERAERRDPVLPEELQEAYENCLPYYYTLAAHTIVPS
                     "
BASE COUNT          545 a          986 c          961 g          641 t
ORIGIN      
        1 gtgcggcgca tcaccgggcg catccaagag ttgcaggcgc gcattgagga ggaaccgaag
       61 tcgttaggct ggaagatgcg ctcgaagatc ggcgaccgca aacgctggta cgacctaccc
      121 gaagaagtgg agggaggacc ctgaaatgat ctctaggctc ttctctcgag aacccgacac
      181 gcgcatcttt tttgcgaccg atatccacgg ctcagaagtt tgctggcgga agttcctgaa
      241 cgccggcaag cactacgaag ctgacattat ggtcctcggc ggcgacatga cgggcaaggc
      301 gctcgtgccg atagtcgagg agggctcggg gcgctggcac gcatcgctac tggagaatcg
      361 gtacgatttt gagaccgaag aagaactgac ggaattcgaa gatgcggtac gacggcgcgg
      421 ctactatccg ttccgcaccg acccggatga gatgagcgag ctcggatcga acgagaagcg
      481 tcgcgagaag ctctttcacg agcagatgct ggggacgatc gagcggtgga tgcggatggc
      541 cgacgagcgg ctcggggact ccgacatccg ctgcctcgtc tcgcccggta acgacgacca
      601 gttcgaggtg gacgagatcg tggccgccgc aaaacgtgtc gaactggccg agggacgggt
      661 cgtcgagttt ggcggcttcc agatggcttc aaccggctgg tcgaaccgca ccccctggga
      721 tacctaccgg gaggaggacg aggacgccct tctcgaacgg ctgcggaaga tgacctcgca
      781 ggtaaccgct ccacccgaga ggacgatcta caacttccac tgtccgcctt acggctctgg
      841 cctcgacgag gctccggaac taaccgacga tctgcgcccg aagcacgggg gacgctcgat
      901 tgtccccgtc ggatcgaaag cggtgcggga ggcgatagag gagggccagc cagtcctcgc
      961 tttgcacggt cacatccacg aggctcgggg caacacgcgc atcgggcgta cgctttgcat
     1021 caaccccgga agctcctacg agcaggggca gctgctcggc gccgtgataa atctggacgg
     1081 caaagggaaa gtcaagcggt tcgtactaac cagcggctaa ggaggcagtc agcagatggc
     1141 cggcaacacc acagcaggtt ccgatgcggg agatctgttc caacgaaagg ccaccggcct
     1201 cttgagagga tggtcagtgt gggacggctt cctctacgcg gccttctcga tcaatctgat
     1261 caccctcggg ctctggatct tttcctttgg ccccttcatc ccggatggcg cgctcatctg
     1321 ggcgatcatc ctttcgggag tctacctcgt ctttcaggcc atcacctacg cttccctgat
     1381 cgcggtgatg cccagggccg gcggcgacta cgtgtggcag agtcgcgtac tcggcggagg
     1441 tatcggcttc gtactcgcgg taacggggtg gtggttcatc ctgtggcact gggtgcccat
     1501 ctacgcgaac attcttaacg tcgaggtcct cgtgccgctg gcgacgattc tggagttgga
     1561 ctcggtcagc gggttcttca gcgggcctgc cgggcttttc accgcctcga tggtcgtcgc
     1621 gatcctagcc tcggtgtaca tctcactcgg gatgcgcgcc tacgcccgca tccagaagtt
     1681 ctgcttctac ggcggcctgg tagggcttgc gataatgctc gtcctcctgc tcgtcaactc
     1741 gaacgccgac ttcgtcggcg ccttcaactc ccaggcgagc gagctctacg gcgcttcggg
     1801 caacgcctac cagcagacct tagaggccgg cagcatcgag gctgcaggcc cggcgaccct
     1861 cgccctcggc ccgacgctgc tcctgatccc gatgatcgtc ttcttcaacc tgtggtccag
     1921 ctggggcgcc acgctctacg gtgaggtgcg gggggcgtcg gactttcgga agaacatcta
     1981 cgcgatgggc ggggcgctcg tagtgacctc tatcatcgcg gtgatcatgc tcgccctcat
     2041 ctccaagact ttcggctggg acttctatta cgcgtcggcc aacgcctact ggtccggtgc
     2101 ggagaacgcg ccgacttcgg tctggcccta cccaggcctc ttcgcggcct tcttcttcga
     2161 caaccccata ctgcaggtga tcctggttgg cctcctctcg ctgtggttct tcgggtgggt
     2221 cggcacggtc ttcctctcat ccacccgggt cgtcttcgcc acggcgtttg accgggtgct
     2281 ccctgaggcg gtcgccagga cctcgcgcaa cggcgttccc tacgtcgccc tggcgctaat
     2341 gctcatccca tccatccccg taagctacct ctacgctttc ggggagaact tcgctaccta
     2401 taccttggac gccacgctcg tcatcgcgat aactttcttg ggctcggcga tcgcggctgc
     2461 tgtgctgccg tggcgcaggc cggagatcta caacgcctcc ccgatagccc gctacaacgt
     2521 actggggata cccctgatca cggtggcggc gtcggccttc gccatcttcc tggtcttctg
     2581 cctgttcaag tggctgaccg acgcgacata cggcatcaac aacggccagt cgctgatcta
     2641 catggtgggc ctctacgtgg tggcgctact cctgtatgtg ggcttcaggc tctaccggag
     2701 ctcccagggg atggacctga agatggtcta cagcgagatt cccgaggatt aactgacatg
     2761 ctttaggcta caggtttcag gtcttttgcc gcttcctgaa gcctgtagcc tgatactttg
     2821 agccttaact cggtactatc gtgtgggcgg cgagggtgta atagtagggg aggcagtttt
     2881 cgtaggcctc ttgcagctcc tcgggtagca cggggtcccg gcgctcggcc cgctcgatgc
     2941 ctgtgctctg ctgcgctgcc tcgtgccacc cctcccagct cttccacttc tcgacctccc
     3001 ccggctccca ggagagggcg tccggccgga aggctatccc cacgcgctcg cagtaggccg
     3061 agattatgcc cacggggttc tcggagaagg tcatggcgtc cactaccacc gggtcatctc
     3121 ccgcctgctc cgt
//
LOCUS       DASTUV010000185         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_189671, whole genome shotgun sequence.
ACCESSION   DASTUV010000185 DASTUV010000000
VERSION     DASTUV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189671"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFT03_03775"
     CDS             <1..192
                     /locus_tag="VFT03_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4847292.1"
                     /translation="PYELGTGYSHVAFAVEDLDALANRLEESGGVDFESKPHAMGSGT
                     RIFFVKDPDGYRIEFIERP"
     gene            256..897
                     /locus_tag="VFT03_03780"
     CDS             256..897
                     /locus_tag="VFT03_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4847293.1"
                     /translation="MCVIRSNIARVCRDERGYTLPEILTAVSIMGIILAIGIIVLLAL
                     LERWRVEAAADQFAADLRLAHTRASEQLTDWRVVFMHDGTPLGRCSSADYCMVKLKFP
                     YGAGDPTPALADDAPLVQRELPDRTKIKDVTFDPDCSHGDRDAVVPPSSCGSDAAQNG
                     ATRTLEFNSNGTVRTLRPGQSGTVVISSEDGIPTCPVVFNAPTSRIRIGDISY"
     gene            881..1270
                     /locus_tag="VFT03_03785"
     CDS             881..1270
                     /locus_tag="VFT03_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4847294.1"
                     /translation="MTSPIKSTAGYSLVEVMVAMVVLTVAVVPMVGMFDTAIGAADAS
                     ADYDKARACAVQKLEQLKSIPYEAVEGGPQGGVCNPSGFGYSTAAQSIGTDLGDGSGE
                     EGLMMVTVTVEWDGENSYVVSGVVSRW"
     gene            1264..1899
                     /locus_tag="VFT03_03790"
     CDS             1264..1899
                     /locus_tag="VFT03_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847295.1"
                     /translation="MVNLARSQPWKEESGFTLVEVLVSAMMMFGVLFALYAIFDVSVR
                     AFGYGGDRLESVGNARLALGRMEREIRAAYPYDLTSDPPRRHLFLDPTDPTSPAVPTA
                     TRITFGNEIGGDRRIDGSEIIGYYLNGHDLKRSKGGSAQTLVDSVPTDGLRFTPCRSP
                     DDCPPALAITDEAEIRLLRIEMAVEVRRRGQDPTRQTLVTDVYLRNRGDQP"
     gene            1896..2315
                     /locus_tag="VFT03_03795"
     CDS             1896..2315
                     /locus_tag="VFT03_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847296.1"
                     /translation="MIRRLLHEERGMALGLAVIVVVIVGVMGAGLLTFVATDLEAIVA
                     VNRGEQAFELAEAGIEVAKAHLANDSSQADWSSGELHMAGMAEGSVTVTVEYHAESGS
                     FEAVSTGEYRETSRKIEATFSLEEGAPKLLSWRELYE"
BASE COUNT          474 a          725 c          724 g          417 t
ORIGIN      
        1 ccctacgagc tcgggacagg atacagccat gtcgccttcg ccgtcgaaga ccttgacgcc
       61 ctggcgaaca ggctagaaga atccggcggt gtggacttcg agagcaagcc gcacgccatg
      121 ggcagcggga ccaggatctt cttcgtcaag gatcccgacg gctaccggat agaattcatc
      181 gagcgccctt aacgtaccct acgcgctctg acgagcccgc taaagtccgc ccgtatccgc
      241 gtcgaaaagt aggccatgtg cgtgatacgg tcgaacattg cccgcgtttg tagagacgag
      301 cgtggctaca ccctgcccga gatcctgacg gccgtgtcca tcatgggcat cattctcgct
      361 atcgggatca tcgtcttgct cgccctgctg gagcgctggc gtgtcgaggc tgccgccgac
      421 cagttcgccg ccgacctgcg actcgcccac accagggctt ccgagcaact gactgactgg
      481 cgggtggtgt tcatgcacga tggcaccccc ctcggccgtt gctcttcggc agattactgt
      541 atggtcaaat tgaaattccc ttacggtgcc ggcgacccca ctcccgcgct ggctgatgac
      601 gcccccttgg tccagcgaga gctgcccgac aggacgaaga tcaaggatgt caccttcgat
      661 cctgattgct ctcacgggga cagggacgcc gtcgttccgc caagctcctg cgggtccgac
      721 gctgcacaga acggcgccac acgcaccctc gagttcaact ccaatggcac ggtcaggacc
      781 ctcagaccag gccagagcgg taccgtcgtg atcagctcgg aagacggtat cccgacgtgt
      841 ccggtcgtct tcaacgcccc gacctcgagg ataaggatcg gtgacatctc ctattaagag
      901 caccgcggga tactcacttg tagaggtgat ggtggcgatg gtggtcctca ccgtcgccgt
      961 cgtaccaatg gtaggcatgt tcgacacggc gatcggggcc gctgacgcga gcgccgacta
     1021 cgacaaggcg cgggcgtgtg ccgtacagaa gctggaacag ttgaagagca ttccgtacga
     1081 ggccgtggag ggtggcccgc agggcggcgt ctgcaaccct tccgggttcg gatactcgac
     1141 agccgcccag tccattggta ccgacctcgg cgacggttcc ggggaggagg ggctgatgat
     1201 ggtcaccgtt acggtcgagt gggacggcga gaactcctac gtcgtttcag gggtcgtgtc
     1261 cagatggtga acctggcaag gagccagccg tggaaggagg agagcggttt cacgctcgtg
     1321 gaggtactcg tctccgcgat gatgatgttc ggagtgctct tcgccctgta tgcgatcttc
     1381 gacgtgagcg tgagggcctt cggttacggc ggtgacaggc tcgagtccgt cgggaatgcc
     1441 aggctcgccc ttggcaggat ggaacgtgag atcagggccg cctatccgta cgacctgaca
     1501 tcggaccccc ccaggagaca cctgtttctg gaccccacgg atcccacaag tcccgcagtg
     1561 cctacggcaa cccgcatcac cttcggcaac gagatcgggg gagaccgacg tatcgatggc
     1621 tccgaaatta taggctacta cctcaacggc cacgacctga aacgatccaa gggcggcagc
     1681 gcccagacgc tcgtagattc ggttcccacc gacggactgc gctttactcc gtgcaggagt
     1741 cctgacgact gtcctccggc cctcgctatc actgatgagg cggagatcag gctcctgcgc
     1801 atagagatgg ccgtcgaagt gaggcgccgg ggtcaagatc caaccaggca gacgctcgtg
     1861 accgacgtct acctcagaaa ccgcggagac caaccatgat ccggcgcctc ctgcacgaag
     1921 aaaggggtat ggctctagga ctcgcggtca tcgtcgtggt gatcgtcggc gtgatgggag
     1981 ccggccttct gaccttcgtc gccacggacc tggaggccat cgtggcagtc aacaggggag
     2041 agcaggcctt cgagttggcc gaagcaggga tcgaggtcgc caaagcacac ctcgcgaacg
     2101 actccagcca ggccgactgg tcctcggggg aactccacat ggccggcatg gctgagggct
     2161 ccgttaccgt tacagtcgaa taccacgccg aaagcgggtc attcgaagca gtctccacag
     2221 gcgagtaccg ggagaccagc cgcaagatcg aagccacctt ctccctcgaa gaaggcgcgc
     2281 ccaagctcct gagctggcgg gaactctacg agtgaaacca tagaaagaat cgtcaatgaa
//
LOCUS       DASTUV010000186         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1490534, whole genome shotgun sequence.
ACCESSION   DASTUV010000186 DASTUV010000000
VERSION     DASTUV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490534"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..2117
                     /locus_tag="VFT03_03800"
     CDS             171..2117
                     /locus_tag="VFT03_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEU4847297.1"
                     /translation="MLKFSAFMAKKSRTARLAAQLTGLLSREEWVYVLSLLVPLVAYD
                     VVLKVVRVATQFEVPGPLGFLDQVRSDLLFNLGYAALWICLFAVVRGGIGRWVLLVLF
                     HLSAVVIVATTTSVHVFFEKTGSMLGFTFVLNSLSSLGDIWKVITSETTIMHWIVVPV
                     ALVYGTVGPAIITRLVTHGWHLPTGGATRAATNPLVACAAALALLSLSLLPSATGAGN
                     AFSRDAVANMVVGELATPEIDTKLAAGSLPTDTRFVETPQTSRRNVVIVFLESTRARS
                     TTPYNEDLNTTPFLLWLSSESLMFERAYTVVPHTSKALVASLCGVSPPLDTERTESEP
                     GAIPARCVPELLEDHGYRTAFFQSATENFERRPQLLENLGYEDYFTTEDMSKEGYDKA
                     NYFGYEDDIMLEPSEEWLQQNREEPFLATYLTVTAHHNYVVPETYGEREFARDDELNR
                     YLNTVRYQDFFLRNLFDQYRELGLYDETTFIVFGDHGEGFDEHGLKQHDNTIYEEGLR
                     IPFFIHQPGRWEGGEGVEPAVNELDILPTVADLLGYEVRGVYPGSSMLSPPEDRTLMA
                     SCYHEHTCLASISGDEKYIYHYGNKADEYFDLSSDPYERHNIIEQQSEEKIKALRDDL
                     LSWESRVTASYELQAAGKKKAAQE"
BASE COUNT          442 a          760 c          710 g          403 t
ORIGIN      
        1 atacagcatc ggtcaggccg agatgcggcc tcgacgatgt cgttctccgc gtcaaagact
       61 ggtccccact tcccgctttc ccgtatactt ttgtagatta ttacccgatt atgccccggt
      121 caatcatccg aaggctcgcc ggatggcact aacggagctc tgtaaaacct ttgttaaaat
      181 tctccgcgtt catggcgaag aagagcagaa cagcgcgcct cgcggcgcaa ctgacgggtc
      241 tcctgagccg cgaggagtgg gtctacgtgt tgagcctgct cgtacccctc gtcgcctacg
      301 acgtagtcct caaggtggtg cgcgtcgcga cccagttcga agtgcccggc cccctcgggt
      361 tcctcgacca ggtacgctcg gacctcctgt tcaacctggg ctacgcggcg ctctggattt
      421 gtctcttcgc ggtggtgcgg ggcggcatcg ggcggtgggt tctcctcgtg ctcttccacc
      481 tctcggccgt ggtgatcgtc gcgacgacca cgagcgtcca cgtcttcttc gagaagaccg
      541 ggtccatgct cgggttcacg ttcgtgctga actcgctgtc ctcgctaggg gacatctgga
      601 aggtcataac gtcggagacc acgatcatgc actggatagt ggtcccggta gcgctcgtct
      661 acgggaccgt gggccccgcg ataatcaccc gcctcgtcac ccacggctgg cacctcccga
      721 ccggcggtgc cacgcgggcg gctacgaacc cgctggtcgc gtgcgcggcg gcgctcgctc
      781 tcctctccct ctcgctgctg ccgagcgcga cgggggctgg caacgccttc tcgagggacg
      841 ccgtagcgaa catggtggtc ggcgagctcg ccacgccgga gatcgacacg aagctagccg
      901 ccggctcgct gccgacggac accaggttcg tcgagacccc gcagacgagc aggcgcaacg
      961 tggtcatcgt gttcctcgag tcaactcgcg cgcgttccac gacgccctac aacgaggacc
     1021 tgaacactac cccgttcctg ctctggctct cctcggagag cctgatgttc gagcgggcat
     1081 acacggtcgt gccccacacc tcgaaggcgc tcgtcgcttc actctgcggg gtgtccccgc
     1141 ccctcgacac cgagaggacc gagtcggagc caggcgccat ccccgcccgg tgcgtgcctg
     1201 agctgctcga ggaccacggc taccggaccg cttttttcca gtcggcgaca gagaacttcg
     1261 agcgacgtcc gcagctcctc gagaacctcg gctacgagga ctacttcacg accgaggaca
     1321 tgagcaagga gggctacgac aaggcgaatt acttcggcta cgaagacgac atcatgctcg
     1381 agccgagcga agagtggctc cagcagaacc gcgaagagcc gttcctggcg acgtacctca
     1441 ccgtcacggc ccaccacaac tacgtcgtcc ccgagaccta cggcgagcgg gagttcgcca
     1501 gggacgacga gctcaaccgc tacctgaaca ccgtccgtta ccaggacttc ttcctgcgca
     1561 acctcttcga ccagtacagg gagttgggac tgtacgatga gacgaccttc atagtcttcg
     1621 gggaccacgg ggaaggcttc gacgagcacg gcctcaagca gcacgacaac accatctacg
     1681 aggaaggcct gcgcataccc ttcttcatcc accagccggg gaggtgggaa ggcggcgagg
     1741 gagtcgagcc ggctgtcaac gagctagaca tcctgccgac ggtggcggat ctgctcggct
     1801 acgaggtccg cggcgtgtat cccggatctt ccatgctctc gccaccagag gaccgcacgc
     1861 tcatggcgag ctgctaccac gagcatacgt gcctggcgag catcagcggt gacgagaagt
     1921 acatctacca ctacggcaac aaggccgacg agtacttcga cctgtcgagc gatccctacg
     1981 agcgccacaa catcatagag cagcagagcg aggagaagat aaaagccctc cgcgacgacc
     2041 tgctctcgtg ggagagcagg gtcacagcct cctacgaact ccaggccgcc ggcaagaaga
     2101 aggcggcgca ggagtagctc ggtggccagg gcgcggcgca taacctagac tattccgcgt
     2161 cacgggaagg cgagatctcg ggaggacgga tgggtgggct gctcgaaggc aagaaggtac
     2221 tggtaacggg tgtcctcgac cggcgctcca tcgccttctc agcagcgcgc ctggcctcgg
     2281 agcagggcgc agaggttgcc ttgagcagct tcggg
//
LOCUS       DASTUV010000187         4108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1522118, whole genome shotgun sequence.
ACCESSION   DASTUV010000187 DASTUV010000000
VERSION     DASTUV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522118"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFT03_03805"
     CDS             <1..205
                     /locus_tag="VFT03_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU4847298.1"
                     /translation="LALEDMGLEPHQVAMVGDDAEADVAGAQAAGLIGIQVRTGKYHP
                     EDEAREADLVLDSIAELPAAVGL"
     gene            266..664
                     /locus_tag="VFT03_03810"
     CDS             266..664
                     /locus_tag="VFT03_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU4847299.1"
                     /translation="MKVHEIPVRTSQRYELVSITDEVRRTVRESGMEEGICLISSTHT
                     TAAVTVNEDYDRDVPRDLAAACEALMESLDVRFEHAEGNSDSHFLTSLFGGSQQLLVR
                     GGELDLGRWQGVFLAEFDGPRQRTVRVTVF"
     gene            772..2169
                     /locus_tag="VFT03_03815"
     CDS             772..2169
                     /locus_tag="VFT03_03815"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4847300.1"
                     /translation="MNAWVKGGLALGGLVAGLGTLRKVLNPKPRYAPWEKPPYGDFDK
                     KVLVLGGGFGGYTAAKDLCELIEDRDDVGILMLAKNNFFTFWPMVPGIVSSDIDARNV
                     AQPLRRALITAGASFRRAEGKKLDLDRKVVVADGGIEFPFDHILISLGGLPNFFGIPG
                     VEEHSLTMRGVEDAERIRNRVIERFEEVSLIRGEVPESKLTFVVIGGGATGVEVASEI
                     HTLVHENLAPDYPNIDPHRVRIILLEGLPNILPELDPALRKAARARMVNQRIEVRTNA
                     MAEEITDNCVKLKGGSTIDSENVIWTAGNRPNLGIEAPGLPVDERTGIKVDEYLRVEG
                     YENVWAIGDCAAVQDVRAGQDGKIVPPTAQAAVQQGHVVARNILAAIDGKQDVMKRFE
                     YRPIGQLVELGSDFAVNEVMGVRFTGHLAAIFWRLAYLVRLNSPQSKVRIAADWIVGL
                     FLRPAVTQIRGTSER"
     gene            2228..2803
                     /gene="pgsA"
                     /locus_tag="VFT03_03820"
     CDS             2228..2803
                     /gene="pgsA"
                     /locus_tag="VFT03_03820"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU4847301.1"
                     /translation="MRLTLANQLTLVRIVAVVPLMIALYLPFDWARWVALAIYVAAIL
                     TDYFDGIVARRSNEVTGFGKLMDSIADKALIVSVFFALVGEGSMAAWMAAIMVIREFA
                     VTGLRMVALEAGEVIAANRWGKAKMNAQSLAVFILLLGEPGAAGWRGVMAEIGWWAML
                     VAVLLTLFSGWSYLRDTPRILSITSKRDQRL"
     gene            3141..3986
                     /locus_tag="VFT03_03825"
     CDS             3141..3986
                     /locus_tag="VFT03_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847302.1"
                     /translation="MADEPSKRLRPAEVEMILRRAAELNSRRWSRDDDGTPAVSPEVL
                     VQVAAAAGIPERDVRRAMWDLASEKTAEPHTLAWKTYGPARLRAVREVERSPRKTQDH
                     LESLLRLEQGLKLRRKTESSSIWDSGDLLGAVRRTLDFSGDRALLKTESVELRVEELD
                     ADRCDANLTADMSNQRAEYLSIGGIVGVTLAVLFILAGAQSPLFFLGVIPALAAPVFA
                     LKFAYRRACGEIRRSLEDLLDAAEEGPPQEEEPPPDRGDRPPGQIRGLRPIPRFVPQQ
                     QREGE"
BASE COUNT          763 a         1267 c         1369 g          709 t
ORIGIN      
        1 cctggctctc gaggacatgg ggctggagcc tcaccaggtc gccatggtag gggacgacgc
       61 ggaggctgac gtggctgggg cacaggcggc ggggctcata gggattcagg tcaggacagg
      121 caagtaccat cctgaagacg aggcgaggga ggcggatctg gttctcgaca gcatcgcgga
      181 gctacccgcg gccgtcgggt tataggagga cggtcggaag ctcgtcttcc cggcgtccga
      241 gcaatgggta caaaaggagt tgcgcatgaa ggtccacgag atacctgttc gaaccagcca
      301 gcgctacgag ttagtctcga tcaccgacga ggtgcgccgc accgtccggg aatcggggat
      361 ggaggagggt atctgcctga tctcctcgac ccacacgacc gccgccgtga ccgtcaacga
      421 ggactacgac cgcgacgtgc ctcgcgacct cgcggcggcc tgtgaggcgc tcatggagtc
      481 gctggacgtg cgcttcgagc acgccgaggg caacagcgac tcgcatttcc tcaccagcct
      541 cttcgggggg tcgcagcagt tgctcgtgcg gggaggggag ctggacctgg ggcgctggca
      601 gggcgtattc ctggcagagt tcgacggccc gcggcagagg accgtgcgcg tcacggtctt
      661 ctaggcgcgt gggacggatg ttttctgggc acacctttag gtaagataag taacgggttg
      721 gcaggctgac gtctcgatgc ccggatgacg tacagagcgc tggaggtcgg tatgaacgca
      781 tgggtaaagg gggggttggc gctcggcggt ctcgtcgccg gcctcggaac gctcaggaaa
      841 gttctgaatc ccaagcctcg ctatgctccc tgggagaagc ccccctacgg agacttcgat
      901 aaaaaggtgc tggtgcttgg aggcggcttc gggggctaca cggcggccaa ggacctctgc
      961 gagctgatcg aagaccggga cgacgtcggg atactgatgc tggccaagaa caacttcttc
     1021 accttctggc cgatggtgcc agggatagtc agcagcgaca tcgacgcccg caacgtcgcc
     1081 cagcccctga ggcgagccct gatcaccgcc ggagccagct tccggcgcgc cgagggcaag
     1141 aagctagatc ttgacagaaa agtcgtggtg gctgatggcg gtatagagtt ccccttcgac
     1201 cacatcctta tctcgctggg tggcctgccc aacttcttcg gcatccccgg cgtggaggag
     1261 catagcctca caatgagggg cgtcgaggac gccgagcgta tccgcaaccg ggtcatagaa
     1321 cgcttcgagg aggtctcgct catcaggggt gaggtgcccg agtccaagct gaccttcgtg
     1381 gtaatcggcg gcggcgcgac aggggtcgag gtggcctcgg agatccacac cctcgtacac
     1441 gagaacctgg ccccagacta cccgaacata gacccgcacc gcgtccgcat tatcctgctc
     1501 gaaggtctcc cgaacatact gccggagctg gatcccgcgc tgcgcaaggc cgcccgcgcc
     1561 aggatggtca accagcgcat cgaggtcagg acgaacgcca tggccgagga gataaccgac
     1621 aactgtgtga agctcaaggg cggcagcaca atagattcgg agaacgtgat ctggacagcc
     1681 ggcaaccgtc cgaacctcgg catcgaagct cctggccttc ctgtggacga gcggaccggc
     1741 ataaaggtgg acgagtatct gcgcgtcgag ggttacgaga acgtctgggc gataggagac
     1801 tgcgccgccg tccaggatgt ccgcgcaggg caggatggca agatcgttcc gccgacagcg
     1861 caggccgcgg tccagcaagg ccacgttgtc gcccgcaaca tcctggccgc catcgacggc
     1921 aagcaagacg tcatgaagag attcgagtac cggcccatag ggcaactcgt cgagctcggg
     1981 agcgacttcg ccgtcaacga agtgatgggc gtgcggttca cgggacacct cgctgcgata
     2041 ttttggcgcc tggcctacct ggttcgcctg aacagcccgc agagcaaggt gcgcatcgcc
     2101 gcggactgga tcgtcggtct cttcctgaga cccgccgtca cccagatccg ggggacctcc
     2161 gaacggtgag ccctggacac acgtcggaga gagggcgtag cgtgttgcgc gggatcggag
     2221 gatagagatg cgcctgaccc tcgccaacca gttgacgctg gtcaggatcg tggcggtggt
     2281 gccgctcatg atcgcgttgt atttgccttt cgactgggcc cgctgggtcg cgctcgcgat
     2341 ctacgtggcg gccatcctca ccgactactt cgacgggatc gtcgcccggc gatccaacga
     2401 ggttacgggt ttcggcaagc tgatggactc gatcgcggac aaggccctga tagtctccgt
     2461 gttcttcgcc ctcgtgggcg aggggtcgat ggcggcctgg atggccgcga tcatggtgat
     2521 ccgggagttc gccgttacgg gtctccgcat ggtcgccctc gaggccggcg aggtcatcgc
     2581 cgccaaccgc tggggcaagg cgaagatgaa cgctcagagc ctcgccgtgt ttatcctgct
     2641 tctcggggag ccaggggccg ccggatggcg cggcgtgatg gcggagatcg gctggtgggc
     2701 aatgctcgtc gcagtgctcc tcaccctgtt ctcggggtgg tcctatctga gggacacccc
     2761 gcgtatactc tccatcacct ccaagcgaga tcagcgcctc tagaacctgt gacggctcag
     2821 cttccctcgg atactagggt aggcggtttc tcggagcacg ctcacgtagc gcgtggccgc
     2881 tgccccttga accggtccgt caactcgtag tcgaagagaa atttttccct ctcggcgtca
     2941 cgattcttta cgctctcgag catctcgttg agatgctctg gcgtggttaa tcgagtcgcg
     3001 ccatgaggag agcttgccgg cgacggttgg gctagagtct gtgcccgaaa gtcgtcctca
     3061 ctcttcgtgt tttggataac tcatccgtcg actatagtgg tcggcgccca gagcacattt
     3121 tctgtagaat cacgacccat atggcggacg agccgagcaa acgcctgaga cccgccgagg
     3181 tcgagatgat actgcgccgc gccgcggagc tgaattcccg ccgctggtcg cgcgacgatg
     3241 acggcacacc cgccgtctca cccgaggtcc tggttcaggt tgcagcggcc gccggcatcc
     3301 cggagcggga cgtccgaaga gcaatgtggg acctggcatc ggagaagacc gctgagccac
     3361 acacccttgc ctggaagacc tacggccccg cgcggctccg ggccgtcagg gaggtcgagc
     3421 ggtccccacg taagacgcag gaccacctcg agtcactgct gcggctcgag caggggctca
     3481 agctgcggcg caagacggag agcagctcga tctgggactc gggggacctg ctcggcgcgg
     3541 tgcgccggac gctggacttc tcgggcgaca gagcgttgct caagaccgag tcggtcgagc
     3601 tccgcgtcga ggagctcgac gcggaccggt gcgatgcgaa cctcaccgcc gacatgtcca
     3661 accagcgtgc ggagtacctc tcgatcgggg gtatagtcgg ggtgacgctc gcagtgctct
     3721 ttatcctcgc cggggcgcag agcccgctgt tcttcctcgg cgtcatccct gcgctcgcag
     3781 cccccgtctt cgccctcaag ttcgcctacc gcagggcatg cggcgagatc cggcgcagcc
     3841 tcgaagacct cctggacgcc gccgaggagg gaccgccgca ggaggaagag ccgccccccg
     3901 accgcggcga ccgcccacca ggccagatcc ggggcctcag acccatcccg aggttcgtcc
     3961 cccagcagca gagagagggc gagtaaatag catttcagca cgctccggct acgccgtcgc
     4021 ttctgcaaag ccgtctcgaa aaacgtgatc cgggtgtgtg cccaacggca gcagagtaat
     4081 ctgaaatgct gacaagcgag gccgcaag
//
LOCUS       DASTUV010000188         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1440693, whole genome shotgun sequence.
ACCESSION   DASTUV010000188 DASTUV010000000
VERSION     DASTUV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440693"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VFT03_03830"
     CDS             complement(<1..1281)
                     /locus_tag="VFT03_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU4847303.1"
                     /translation="MSSEPRHLHPAAMLIDAVKTVRRSLSAFVIPGIAFLMSRGFDAQ
                     TIVLVLFGALVVTALAAFWGFLSWRATTYEVAGGVFRLRWGVVQKNERAVPLDHIQSV
                     DTVQGIIQRLFNVVEVRIETAGGGASEPDASLAALDRADAEALRREIAGSRREPVETE
                     TAGPTVVRKLGTRDLLIAGATSGQIGVALSLIAVASQLFDNFLSQNLAQRLIETFAPR
                     SVTTALLYVFILGLVAWLLAIGGTVLAHTGFTLSRDGDFLYIRRGLLERREATIPLAR
                     IQAVRIVEGLLRQPFGLASLRVESAGYGQDAGVSTTLFPLLPRKEVHRLLLEVAPEFA
                     VAPPLNALPRRALRRYVFRSTVPFLFLISAATLFSHLVFDLAAWVFAALLLLLPAALY
                     GRLRYRDAGWALEEDRLVVRSRLLGRTTAIAPRRR"
     gene            complement(1271..1750)
                     /locus_tag="VFT03_03835"
     CDS             complement(1271..1750)
                     /locus_tag="VFT03_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU4847304.1"
                     /translation="MKAEPAERLDSRAKTLWRITGALGALPLVAGGAFVSWILLRVAG
                     IPLLFGILPFLAALALFVALASVVPDLRWRRWRYEIRDDEVDLQRGILWIRRTLVPLA
                     RIQHVDTQSGPLQRRFGLATVVFYTAAGANQIPELSAPVAARTRDRISDLTREQDEL"
     gene            complement(1755..2798)
                     /gene="rlmN"
                     /locus_tag="VFT03_03840"
     CDS             complement(1755..2798)
                     /gene="rlmN"
                     /locus_tag="VFT03_03840"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU4847305.1"
                     /translation="MRAASFLPDVGEVLEERGEPAYRLEQAYLALTRSLVRNWEEATN
                     LPKGLRAALSEGAPAASLELRRVSQATDGTRKYLFLTRDGHAIETVMIPEKGRHTVCI
                     STQVGCPMACKFCATGLLGIKRNLDAREIAEQVFVVARDIEPGRVSNVVVMGMGEPFL
                     NYEQTLSALKTLNDPDGFALAARHIAASTSGLVDRIRRFADEPEQFHLAISLHNPFED
                     ERRALMPVAARHSIPELMDAARYYVERTRRKLFFEYTLLAGVNDQPRHVEALGTLLDH
                     PLYHLNLLRFNWTDTGFSATSRRDAKAFLRRAKAFGLSATLRPSRGQDIDAACGQLAA
                     KDLRGSPAVPLSS"
     gene            2858..4006
                     /locus_tag="VFT03_03845"
     CDS             2858..4006
                     /locus_tag="VFT03_03845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEU4847306.1"
                     /translation="MTPIDLLIALFVVLTVVRGARTGLLAGAFSLVGVVLGASIGSRV
                     APYLMPEGENPLFGAGITLVSILAFAALGEIAARAAGGALRNRLSSPASQALDGLGGA
                     TLGLVLSLMLVWAIGLFALQAPTLAGVHPAVKESRILQLLNERMPAALLTKAVAELNP
                     LPQIRGPEADVAVPNSRLAQDPEVLAAGSRTVRVTSIACGYGVEGSGWVAAPNLVVTN
                     AHVVAGGAATRVQPGGTGPNLRAEVIVFDYKNDVAVLRVGGLGLDPMPLAAPRVGEAT
                     AVLGFPENGPLNIQPARTGSTRRVISGDAYNQGPVERTVTSFRVYVRPGNSGGPVVNG
                     DGEVVATVFASRANSNDSGYGIPSQLVQRRLATAAGSTEPVNTGGCVS"
BASE COUNT          645 a         1330 c         1438 g          598 t
ORIGIN      
        1 ccggcgccgc ggtgcgatgg cggtggtacg gcccaggagc cgcgaacgaa cgacgagacg
       61 gtcctcctcg agcgcccatc cggcatcccg gtagcgaagc cggccgtaga gggcggccgg
      121 tagaaggagc agcagcgctg cgaagaccca ggcggcgagg tcgaagacga ggtgagagaa
      181 aagcgtggcc gcggagatca ggaacagaaa cggtacggtg gatctgaaga cgtagcgccg
      241 gagggctcgc ctcgggaggg cgttgagcgg cggagcgacg gcgaactctg gggcgacttc
      301 gagcaggagc ctgtggacct ccttgcgtgg caggagcggg aagagggttg tcgagacgcc
      361 cgcgtcctgg ccgtagccgg cgctctcgac gcgcagggag gccaggccga agggctggcg
      421 gaggaggccc tccactatgc ggacggcctg gatgcgggcg agcggtatcg tcgcctcccg
      481 tctctccagg agcccgcgcc ggatgtacag gaagtccccg tcgcgcgaga gcgtgaagcc
      541 ggtgtgggcc aggaccgtcc caccgatggc caggagccag gcaacgaggc cgaggatgaa
      601 tacgtagagc agggcggtgg ttaccgagcg cggcgcgaag gtctcgatga gacgctgggc
      661 caggttctgc gagaggaagt tgtcgaatag ctgggaagcg acggcgatca gggagagggc
      721 caccccgatc tggccgctgg tcgctcccgc gatgaggagg tctctcgtcc cgagtttccg
      781 gacgacggtg ggtccggcgg tctccgtctc gacgggctct ctcctggagc ccgcgatctc
      841 acgccgcaga gcctcggcgt cggcgcggtc cagggccgcg agcgaggcgt cgggctcgct
      901 ggctccgccc cccgccgtct ctatcctgac ctcgacgacg ttgaagaggc gctggattat
      961 cccctgcacc gtgtcgacgg actggatgtg atccaggggt acagcccgct cgtttttctg
     1021 gacgacgccc caccgcaggc ggaagacgcc gcccgcgacc tcgtaggtgg tggcccgcca
     1081 ggagaggaag ccccagaagg ccgccagcgc cgtcacgacg agcgccccga acaggacgag
     1141 gactatagtc tgcgcgtcga acccccggct catcaggaac gcaatgccgg ggatgacgaa
     1201 ggcgctcagg gagcgccgga cggtcttgac ggcgtcgatg agcatcgccg ccgggtggag
     1261 gtggcgcggc tcagagctca tcctgctccc tggtgagatc ggagatccta tcgcgtgttc
     1321 gggctgcgac gggcgcagag agctcgggga tctggttggc cccggcagcc gtgtagaaga
     1381 ccaccgtagc caggccgaac cggcgctgca gcgggccgct ctgggtgtcc acgtgctgga
     1441 tgcgcgcgag cgggacgagg gtgcgcctga tccagaggat cccacgctgc aggtccacct
     1501 cgtcgtcgcg gatctcgtag cgccaccgcc gccacctgag gtcgggcacc acgctcgcga
     1561 gcgcgacgaa cagcgccagc gccgccagga acggcaggat gccgaacagg agcgggatgc
     1621 cggcgacgcg aagcaggatc cagctaacga acgcgccgcc tgcgacgagc gggagcgccc
     1681 cgagggcgcc tgttatgcgc cagagggtct tcgcccgcga gtcgagcctc tctgcgggtt
     1741 cggccttcaa gtacctagga agagagcggg acggcgggac tgcccctgag gtccttcgcg
     1801 gcgagctggc cgcaggcggc gtctatgtcc tgacctctgc tcggtcgcag cgtcgcggag
     1861 aggccgaacg ctttagcgcg ccgcaagaac gccttcgcgt cgcgcctgga ggtcgcggag
     1921 aagcctgtgt cggtccagtt gaagcggagg aggttgaggt ggtagagcgg gtggtcgagg
     1981 agagtgccga gcgcctcgac gtgacggggt tggtcgttca cgcccgcgag cagggtgtac
     2041 tcgaagaaga gcttgcggcg tgtgcgctcg acgtagtagc gggccgcgtc catgagctcg
     2101 gggatggagt ggcgggctgc gacgggcatc agggccctgc gctcgtcttc gaaagggttg
     2161 tggagcgaga ttgccaggtg gaactgctcc ggctcgtctg cgaaacgcct tatcctgtcg
     2221 acaagcccgc tggtcgaggc ggcgatgtgc cgcgccgcca gagcgaaccc gtccgggtcg
     2281 ttcaaagtct tgagcgctga gagcgtctgc tcgtagttca ggaacggctc ccccatcccc
     2341 atcactacga cgttcgagac cctgcccggc tcgatgtcgc gggcgacgac gaagacctgc
     2401 tcggcgatct cgcgcgcatc caggttgcgc ttgataccga gcaggccggt cgcgcagaac
     2461 ttgcaggcca tcgggcaccc gacctgggtc gagatgcaca cggtatgccg ccccttctcg
     2521 gggatcatga ccgtctctat ggcgtgcccg tcgcgggtga gaaagagata cttcctcgtc
     2581 ccgtctgtcg cttgcgagac gcgccgcaac tcgagcgacg ccgcgggtgc cccctcggag
     2641 agcgcggccc gcaacccttt cggcaggtta gtggcctcct cccagttccg caccagagag
     2701 cgggtcaggg cgaggtaggc ctgctcgagg cggtaggcgg gctcgccccg ctcctcgagt
     2761 acctctccta catcgggaag aaagctcgcc gctctcatcg cccacggatt atataggcca
     2821 cccgcgcagg cgtcgctcca gcggtagaat ctgccggatg acgcccatcg acctgctcat
     2881 tgcgctattc gtcgttctga ccgtggtgcg cggcgcccgg accggcctcc tcgccggcgc
     2941 cttctcgctc gtgggggtag tgctaggtgc ttctataggt tcgcgggtag cgccgtatct
     3001 gatgccggag ggcgagaacc cgctcttcgg cgcggggatc acgctggtca gcatcctagc
     3061 cttcgcggcg ctcggggaga tagcggcgcg cgccgccggc ggcgccctga gaaaccggct
     3121 ctcgagccct gcctcacagg cgctcgacgg cctcgggggc gcaacgctcg ggctggtgct
     3181 ctcgctcatg ctggtctggg ccatcgggct cttcgccctc caggctccca cgctcgcggg
     3241 cgtgcacccg gccgtgaagg aatccaggat cttgcaactc ctgaacgagc ggatgcccgc
     3301 cgcgctgctg acgaaggccg tcgcggagtt gaacccgctc ccccagatcc ggggacccga
     3361 agccgacgtg gccgtcccca acagccgcct cgcgcaggac cccgaggtgc tcgccgccgg
     3421 gtctcggacg gtgcgcgtga ccagcatagc ctgcggctac ggcgtcgagg gctcgggctg
     3481 ggtcgccgcc cccaacctcg tcgtcaccaa cgcccacgtg gtcgcgggcg gggccgccac
     3541 cagggtccag ccaggcggca cggggccgaa tctgcgagcc gaggtgatcg tcttcgacta
     3601 caaaaacgac gtggcggtcc tgcgcgtcgg cggcctcggc ctcgacccca tgccgctcgc
     3661 cgcgccccgc gtgggcgagg cgacagccgt gctcggcttc cccgagaacg gtccgctgaa
     3721 catccagccc gcccgcacag gctcgacgcg gcgcgtgatc tccggcgacg cctacaacca
     3781 gggcccggtg gagcgcaccg taaccagctt ccgcgtctac gtcaggcctg gcaactcggg
     3841 cggccccgtc gtcaacggcg acggtgaggt cgtcgccacc gtcttcgcca gccgcgccaa
     3901 ctcgaacgac tcgggctacg gcataccctc ccaactcgtc cagcgccgcc tggccacggc
     3961 agccggaagc accgaaccag tcaatacagg ggggtgtgtg agttagctgg c
//
LOCUS       DASTUV010000189         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_973267, whole genome shotgun sequence.
ACCESSION   DASTUV010000189 DASTUV010000000
VERSION     DASTUV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973267"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(201..277)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(395..467)
                     /locus_tag="VFT03_03850"
     tRNA            complement(395..467)
                     /locus_tag="VFT03_03850"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(432..434),aa:Ala,seq:tgc)
     gene            573..815
                     /locus_tag="VFT03_03855"
     CDS             573..815
                     /locus_tag="VFT03_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU4847307.1"
                     /translation="MALYEYKCADCEERFDLIRPMSAADDVAECPECGSEDSRRVISN
                     FASITPGASSLSTNPVMDARMASGAGGCCGGGCGCG"
     gene            802..1203
                     /locus_tag="VFT03_03860"
     CDS             802..1203
                     /locus_tag="VFT03_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEU4847308.1"
                     /translation="MAAASERMAQDPDGLPDLALKGIEEFNRGEFYECHEYLEEAWMQ
                     EPRRVRFLYQGILQVGVGFYHLQNGNWRGATGLLRNGTQRLKEFEPDTLGIDVARLVR
                     ESERCLQELEDLGRERVREFDASKIPRVEFV"
     gene            1376..2101
                     /locus_tag="VFT03_03865"
     CDS             1376..2101
                     /locus_tag="VFT03_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4847309.1"
                     /translation="MSEPSAERIKLLSMVDIFEPLSKQDLARLNGQLPDVHLEPGDMF
                     YGPEDQTEKLFILQKGKIRIFRMTPDGREFTLAVVEAGTVFGEMALTAQQLEGAYAQA
                     MESSQVSTMAREDLERLVLEKPEVGLRIMQVLSERLRRLENRLEDATMKDVHARLAGI
                     IVLLVESEGVRTGTGYRISAHYTHERLGTMIGANRVAVTRAFGLLQDEGVVELRRRLI
                     HVTDIEALRRTAAYGDAREDGHE"
     gene            complement(2092..2355)
                     /locus_tag="VFT03_03870"
     CDS             complement(2092..2355)
                     /locus_tag="VFT03_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847310.1"
                     /translation="MCERHVYRLWLEERALFWRAMLAHTELWSGEARRRGRDDVVGRL
                     EKLRARSAAALGGILEDLEESRDLDGENGSGVGRDPPNGDRYS"
BASE COUNT          570 a          864 c          865 g          534 t
ORIGIN      
        1 tgtgtttacg ggctaacttt acgggctaac aatcgtatcg ttagcaatgc aagttcactt
       61 gagccaaggc cgcgcgtatg ggtcctttcc cttcgccgac cataatgcgt tcccagtgat
      121 tcacaataca ttaccgagtc gcgatgttca tctcgtccta tccaacgggc aagcgcagga
      181 ctaggcttta gaaacgcacg ctcggtagcc cggctgcctc tcctagaagt ccgtggcttt
      241 gcgcgctcgc ctcacgacga tgttgccctt ctcgcggcgc cggtgtgccc atccacccga
      301 ccacaactat tgtaataagt ggttcaatcc cgcgtaaggg gtttaaagat aattcttcgc
      361 cgacatccgg tgagttcacc cgacgtttct agagtggagc cgaggggatt cgaacccctg
      421 acctccgccg tgcaaaggcg gcgctctccc aactgagcta cggccccggc tggatcgagg
      481 atgagtttac gcgctgctcc tgtgttcttc aatgcgcgaa gcgcggtaca atgtgttcgc
      541 cgtaaagttg ccgaacgaga ggtgggaacg agatggctct ctacgaatac aagtgcgccg
      601 actgcgagga gcgcttcgac ctcatccgcc cgatgagcgc cgccgacgac gtcgcggagt
      661 gtccggagtg cggctccgaa gactcgcggc gtgtgatctc caacttcgcc tctataactc
      721 ccggcgcctc ctccctctcg acgaaccccg tgatggacgc gcgcatggcc agcggagccg
      781 gcggctgctg cggcggaggc tgtggctgcg gctagcgaga ggatggccca agaccccgat
      841 ggactgccgg acctcgccct caagggcata gaggagttca acaggggcga gttctacgag
      901 tgccacgagt acctcgagga ggcctggatg caggagcctc gccgggtccg cttcctatac
      961 cagggcatcc tccaggtcgg cgtcggtttc tatcatctgc agaacggcaa ctggcgcggg
     1021 gcaacgggtc tcctgcgcaa tggcacccag cgtctcaaag agttcgaacc cgacactcta
     1081 ggtatagacg tcgccaggct agtccgcgag agcgaacggt gtctgcaaga gctcgaggac
     1141 ctggggcgag agcgggtgcg cgagttcgac gcctcgaaga tccctagagt cgagttcgtc
     1201 tagcgcctcc ggctcaaggc gtggttcagt cggagccggc tctctgcgac tatagctcaa
     1261 tggtgcaagt cgatggcccg aaagatgcat tatggcccac catacattaa aataatggag
     1321 acgatctagt agcagatcta gcaatccatt acgacggttc agcggaggat cagcagtgtc
     1381 ggagccgagc gcggagagga tcaagctcct ctccatggtc gatatcttcg aaccgctctc
     1441 caagcaggac ttagcgcggc tcaacggcca gcttccggac gtccacctcg agccggggga
     1501 catgttctac ggtccggaag accagaccga aaagctcttc atactccaga agggcaagat
     1561 caggatattc aggatgacgc ctgatgggcg ggagttcacc ctcgcggtag tagaggccgg
     1621 tactgtgttc ggggagatgg cgctcacggc ccagcagctt gagggggcct atgctcaggc
     1681 tatggagtcc tcgcaggtat ccacgatggc cagagaggac ctcgagcggt tggtgctgga
     1741 gaagcccgag gtgggcttac ggataatgca ggtccttagc gagcggctca ggcgcctgga
     1801 gaaccgcctg gaggacgcca ccatgaagga cgtccacgct cgccttgcgg gcattatcgt
     1861 gcttctggtc gagagcgagg gcgtaaggac cggcacgggc tacaggatat ctgcccacta
     1921 cacccacgag cgcctcggta ccatgatagg ggccaacagg gtggcagtga cgcgtgcctt
     1981 tgggttactt caggacgagg gggtcgtgga gctcaggcgc cggctgatcc acgtgaccga
     2041 catcgaggct ttaaggagaa cagcagctta cggtgatgcc agggaggacg gtcacgaata
     2101 acggtcgccg ttggggggat ccctccctac gcccgagccg ttttcgccat ccaagtcccg
     2161 gctttcctcc agatcttcca ggatgccccc cagcgccgca gctgacctcg cccgcaactt
     2221 ttccaggcga cccaccacat catctctacc ccggcggcgc gcctccccgg accaaagttc
     2281 cgtgtgcgcc agcatcgccc gccagaagag tgcccgctct tccagccaga gccggtagac
     2341 gtgccgctcg cacaaccaca ggccctccag ctcaacgcaa cctttctcca agcagcactc
     2401 gcccttgagt gtacgttcga actcgcagta gagctctcgg ggaacgcgtc ggtaaggatc
     2461 acgtccgtcg acggccatga gtggcacctc cccttcggta gtgcctggca cctaccctgt
     2521 caaaggtcga gccggttcgt ccggggccta caccgggcca gacgacctcc gggagggggg
     2581 agaaggagat cgtcgtagcc cggtatcggt actaccatac tctcctcgag aggctggcgc
     2641 tgtacgccga agcactttgt gctctgtgga acatacagtt atcgtcggca ggagtacagt
     2701 gaagtcgtcc acgtgccacg cgacgatcgc gtagagggca ggtagctgtc cagcactcct
     2761 cctggacccg ggggtagcaa cgcgacctac tacctgccac cgggcgggaa gctcgtaatg
     2821 gagaagttgc gtt
//
LOCUS       DASTUV010000190         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1748717, whole genome shotgun sequence.
ACCESSION   DASTUV010000190 DASTUV010000000
VERSION     DASTUV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748717"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFT03_03875"
     CDS             complement(<1..1007)
                     /locus_tag="VFT03_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4847311.1"
                     /translation="MFGSMRNLKVFSTALLAVLVATAVSCGVGQQPSQGGGGGGGPAK
                     ICMMPKLVGIPYFNASEKGAEEAAKELDVELVYDGPTEAKAALQSQMIEQFIQQQCGA
                     ITVAANDPNALAPAMKKADNAGIATGAWDADVAPDARDVFVNQATFEAIGYKLVDVMA
                     EQTGGKGKFLVITGSLTAPNQVAWLKEMRERMKEKYPDMSIVAVEPGEEDLQKGIDLT
                     KNYLRANPDTAGVFGITSVALPGAAEAVEQLGRGGEVPVTGLSTPNEMKPYLDAGTVE
                     KFVLWNPVDLGYLAVYVADAQIKGDLPKSGTFEAGRLGKVEMIAPDEVLLGPPLVFSK
                     E"
     gene            complement(1067..2026)
                     /locus_tag="VFT03_03880"
     CDS             complement(1067..2026)
                     /locus_tag="VFT03_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847312.1"
                     /translation="MSSAVEAMRHLRFSRELVLLCVLIALVIVMSLLSPLFFTLGNLL
                     NTSRYFVEVGLMALGMTLIIITGGIDLSVGSNLALVSVAVGFTFAAGLPLPVAILFGL
                     MVGVAAGFFNSIFITFLDLHPLVVTLGTFALFQGLAYGLTGARAVSDFPPWFAFFGQA
                     YLLGAVPGQLIIFLVAVVVVWLILSRTSFGRYVYAIGNNEEAARFSGVPIRRVKVAIY
                     SGIGLLVAIASVIYTSRVYTARGDVGLGLELDVISAVVLGGASIYGGSGTIGGTVLGI
                     VIIAILRNGLLLAGVPSTWQLFILGVLLLVAVFLNEFFRRGEE"
     gene            complement(2023..>2426)
                     /locus_tag="VFT03_03885"
     CDS             complement(2023..>2426)
                     /locus_tag="VFT03_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847313.1"
                     /translation="GRNVYAVGGNRRAARFAGLPTDRVRFLAFVLVGVFVGVAAMIYM
                     GRAGSVQTNTGTGLELQVVAAVVIGGTSISGGRGSSLAALTGAILIGVILNGLVLVGV
                     PGIWQQAVLGALILLAVTTDVVRRRLVGDGV"
BASE COUNT          482 a          783 c          761 g          400 t
ORIGIN      
        1 ttctccttcg agaacaccag cggcggtccg agaaggactt cgtcgggggc gatcatctcg
       61 accttaccga ggcgtcccgc ctcgaaagtg cccgacttgg ggaggtcacc tttgatctgg
      121 gcgtccgcga cgtagacggc caggtagccg aggtccacgg ggttccagag gacgaacttc
      181 tccacggtcc ctgcgtcgag ataaggtttc atctcgttag gggtggagag ccctgtcacc
      241 ggcacctcgc ccccgcggcc gagttgctcg acggcctctg ccgcgcccgg cagggccacg
      301 gaggttatgc cgaaaacacc ggctgtgtcc gggttggccc tgaggtagtt cttggtcagg
      361 tcgatgccct tctggaggtc ttcctccccc ggctcgacgg cgacgataga catgtcgggg
      421 tacttctcct tcatgcgctc gcgcatctcc ttgagccagg caacctggtt gggggcggta
      481 agtgaaccgg tgatgaccag gaacttgccc ttgccgccgg tctgctcggc catgacatcg
      541 accagcttgt agccgatggc ctcgaaagtt gcctggttga cgaaaacgtc cctggcgtcg
      601 ggggccacat cggcgtccca ggcgcctgtg gcgattccgg cgttgtcggc tttcttcatg
      661 gcgggggcca gggcgttcgg gtcgttggcg gccaccgtga tggcgccgca ctgctgctgg
      721 atgaactgct cgatcatctg cgattggagc gcggccttgg cctcggtcgg gccgtcatag
      781 accagctcga cgtctagttc cttcgcggcc tcttcggccc ctttctcaga agcgttgaag
      841 taagggatgc cgaccagctt gggcatcatg cagatcttgg ccggtccacc gcccccacct
      901 ccgccctgcg acggctgctg gcccactccg cacgagacgg ccgtggcgac cagcaccgcg
      961 agcagggccg tcgagaatac cttcagattc ctcatcgaac cgaacacgct actccttccc
     1021 aaagattgcc ttccagagac cgttactcgc tctacccaga catgttctac tcctcgcctc
     1081 tgcggaaaaa ctcgttcagg aacacggcca cgaggagcaa gacgccgaga atgaagagct
     1141 gccaggtgct cggaactccg gccaagagca ggccgttgcg caatatcgcg atgatgacga
     1201 tgccgagtac ggtcccgcct atggtccccg agccaccgta gatgctcgcc cctccgagca
     1261 cgacggcgga gatcacgtcc agctccagcc ccaggccgac gtcgcccctg gccgtataga
     1321 ccctcgaggt gtagatcacg gacgcgatcg ccacgagcag cccgataccg gagtagatgg
     1381 ccaccttgac cctgcggatg ggcacgccag agaaacgtgc tgcctcctcg ttgttcccta
     1441 tggcgtagac gtagcgcccg aagctggtgc gcgagagtat gagccacacg accaccacgg
     1501 cgaccaggaa gataatgagc tggcctggta ccgcgccgag caggtacgcc tggccgaaaa
     1561 aggcgaacca gggagggaag tccgagacgg cccgcgcccc cgtgaggccg taggcgagcc
     1621 cctggaagag ggcgaaggtt cccagggtaa cgacgagagg gtgcaagtcc aggaacgtta
     1681 tgaagatgct gttgaagaat ccggcggcca caccgaccat gaggccgaac aagatggcga
     1741 cgggcaaagg gagtcccgct gcaaaggtga aaccgacggc cacggagacc agcgccaggt
     1801 tcgacccgac cgagagatct atcccacccg tgatgatgat caaggtcatc cccagcgcca
     1861 tgaggcccac ttcgacaaag tagcgcgaag tgttgagcag attgccgagg gtaaagaaga
     1921 ggggtgagag caggctcatt acgatgacca gcgcgatcag gacgcagagc aagaccaact
     1981 cgcgggagaa ccgcaagtgt cgcatggcct cgactgccga gctcatactc cgtcccccac
     2041 gagccgtctg cgcacgacgt cggttgttac ggcgagcagg atcagggctc caagcactgc
     2101 ctgctgccag atgccaggca cccccacaag gacgaggccg ttcaggatga cgcctatgag
     2161 gatcgcaccg gtcagggctg cgagcgagga gccacgtcct cccgagatgc tcgtcccgcc
     2221 tatcacgacg gcggcgacga cctgaagctc caggccagtg cctgtgttgg tctgcaccga
     2281 gcctgcgcgc cccatataga tcattgctgc gacgccgacg aagacgccaa cgagcacgaa
     2341 ggcgaggaac ctgacccgat ccgtgggcag cccggcaaaa cgagccgccc gccgattgcc
     2401 gccgacggcg tagacgttcc ggcccc
//
LOCUS       DASTUV010000191         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1717280, whole genome shotgun sequence.
ACCESSION   DASTUV010000191 DASTUV010000000
VERSION     DASTUV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717280"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFT03_03890"
     CDS             <1..351
                     /locus_tag="VFT03_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4847314.1"
                     /translation="YQVFLNLLDNAIKYSDAGDRVDVGIEEDVVYLTVEVKDTGVGIP
                     PEDLSQLFERFYRVDKNRSRATGGSGLGLAICRQIVEMHGGDISVESEVGVGSTFRVR
                     LPKAPITRSASHAL"
     gene            353..1570
                     /locus_tag="VFT03_03895"
     CDS             353..1570
                     /locus_tag="VFT03_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847315.1"
                     /translation="MSFSMRFRARSAVKCMALLAATLFLAIGCMHSEQERSIPPLSAA
                     GERVDPGLGPEAQPLSSGPGYKGSPSWSPHGGRVAFTVDGYVVDRPVGTGETKHWTTR
                     DFVAQDAEWTSEDRLAILGSASGAVSADGASVYSARSGEESLAVREVVKEVLAMSRGP
                     EGNGLIVAFRAGPGKSGLALLRDNGAVDKVYTSTVGGSVTGLSLSPDGRSVALAARTA
                     VGTFELHTFDLLAGVHRRIALLDRGLEIFGAPQWTKKGICYVVGKEATSIDGGTAPLY
                     DLYSITPGSGGPPEPTPGVGEDFVASSIRVSPDGGRLAVIGRLNPNSPMNLYVLDLAA
                     QDLEAVTTNEDMEIKTGPDDLAWSPGGDSVAIVARGALSDEPRVRAAPKRALLEDFYN
                     LYEIPVGDRGAAR"
     gene            1567..2076
                     /locus_tag="VFT03_03900"
     CDS             1567..2076
                     /locus_tag="VFT03_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847316.1"
                     /translation="MKEAALWISGAAFAFFATAGAFLLLANLGTGPSERNLTPDPQPA
                     EAGPALELSLNQNQLASLRALPGQVLEVGVRNAGDEDLSHVNLTVEVSSENTALSDAL
                     YYRDNVNELPAGSSVPVRFYLDLSQRESPMGAATSAPAAPEPPRKIIEVRATTPEGES
                     AIKTAIVPL"
     gene            complement(2159..>2584)
                     /locus_tag="VFT03_03905"
     CDS             complement(2159..>2584)
                     /locus_tag="VFT03_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847317.1"
                     /translation="VYGPTERLPHAVAYPVGSLGEDAVLLAYVAGDGRTRRRLLVRML
                     RDLKHRGVVRVEAIASDRGASHHVPTSFLLESGWKPVRRGWRRGSPFTLVRTDLGSAV
                     EVGELARGLVGRVKLPKLKAPNPAPGALARARSPQERHT"
BASE COUNT          438 a          868 c          872 g          406 t
ORIGIN      
        1 tatcaggtat tcctcaacct gctggacaac gccatcaagt actccgacgc cggcgaccgt
       61 gttgacgtcg ggatagagga ggacgttgtg tacctgacgg tggaggtgaa ggacaccgga
      121 gtcggcatcc caccagaaga cctgagccag ctcttcgagc gtttctacag ggtagacaaa
      181 aaccgctcgc gcgcaaccgg ggggagtggg ctcgggctcg ccatctgccg ccagatcgtc
      241 gagatgcacg gcggcgacat ctccgtagag agcgaggtcg gcgtgggctc taccttcagg
      301 gtgcgcctcc cgaaagcgcc tatcacgcgc tccgccagcc atgcgcttta gcatgagctt
      361 cagcatgcgc ttcagggcgc gttccgccgt gaaatgtatg gcgctgctgg cggcgacgct
      421 cttcctggcg atcgggtgca tgcattcgga gcaggagagg tccataccac ctctgtccgc
      481 cgcgggcgag cgggtcgacc cgggcctcgg tcccgaggcg cagcctttaa gctccggccc
      541 cggctacaag ggatccccga gctggagccc gcacggcggc agggtagcgt tcactgtaga
      601 cggctacgtt gtggacaggc cagtgggcac aggagagacc aagcactgga cgaccaggga
      661 cttcgtggcc caggacgccg agtggacctc cgaggacaga ctggccatcc tcggctcggc
      721 ctccggcgcg gtgtcggcgg atggggcatc cgtctacagt gcgcgctcgg gtgaagagtc
      781 gctcgcggtc agagaggtcg tgaaggaggt gctggccatg agccgggggc cagaggggaa
      841 tggtctgatc gtcgccttcc gggcgggacc cgggaagagc gggctagcgc tgctccgcga
      901 caatggagcg gtggacaagg tctacaccag caccgtcggg ggcagcgtca ccggcctctc
      961 cctctcgccc gacggcagaa gcgtagccct ggcggcgcgc accgcagtgg gcaccttcga
     1021 gctgcacact ttcgacctcc tggcgggcgt ccaccggcgg atagcgctgc tggatagggg
     1081 cctggagatc ttcggcgcgc cgcagtggac caagaaagga atctgctacg tcgtcgggaa
     1141 agaggctacg tctatcgacg ggggcacggc gccgctctac gacctgtaca gcattacgcc
     1201 tggctccggc ggcccgccgg aacctacgcc gggggtaggc gaggactttg tcgcctccag
     1261 catccgggtc tcgcccgacg gcgggcgcct ggccgtgatc gggcgcctca acccgaactc
     1321 tccaatgaac ctctacgtcc tcgacctcgc cgcgcaggac ctggaagccg tgactaccaa
     1381 cgaggacatg gagatcaaga ccgggccgga cgatctggcg tggtccccag gcggggacag
     1441 cgtagccatc gtcgcgcgcg gggccctctc cgacgagccc cgcgtgcgcg ccgcgcccaa
     1501 gcgtgccttg ctcgaggact tctacaacct ctacgagatc cccgtcggcg accggggagc
     1561 ggcgcggtga aggaggccgc gctctggatc tccggcgcgg ccttcgcttt tttcgccacg
     1621 gcgggcgcct tcctgctgct tgccaacctc ggcacgggcc catccgagag gaacctgacg
     1681 ccagacccgc agcccgccga agccggtccc gcgctggagc tctccctgaa ccagaaccag
     1741 ctggcgtccc ttagagcact acccgggcag gtgctcgagg tcggcgtgcg caacgccggc
     1801 gacgaagacc tgtcgcacgt caacctcacc gtggaagtct cctccgagaa caccgccctc
     1861 tccgacgccc tctactaccg ggataacgtg aacgagctgc ccgcgggaag ctccgtgccc
     1921 gtgcgctttt atctggatct ctcgcaaagg gagagtccga tgggcgccgc cacctctgcg
     1981 ccggccgccc ccgagccacc gcgcaagata atagaggtgc gggccacgac gccggagggt
     2041 gagtcggcta tcaagacggc gatcgtgccg ctctgaaagt caggcttcag gttacaggca
     2101 tcgggttatg ggttattgct gatctcgcag actgtagaag gacctttcac aatccgcttt
     2161 acgtgtgtcg ttcttgaggg ctcctcgccc gggcaagggc gcctggggcc gggtttggcg
     2221 ccttgagctt gggcagcttc acccgcccga ccaggccgcg cgccagctct cccacctcga
     2281 cggcgctgcc cagatcggtg cgcaccaggg tgaacgggct gccgcggcgc catccccggc
     2341 ggacaggctt ccagccgctc tccagcagga acgaggtcgg cacgtggtgg gacgccccgc
     2401 ggtcgctggc gatggcttcg accctcacga cgccgcggtg cttgagatcc cggagcatcc
     2461 ggacgagcaa ccggcggcgc gtgcgcccgt caccggcgac gtatgccagg agcaccgcgt
     2521 cctcgccaag agatcctacc ggatacgcca cagcgtgcgg caggcgctcg gtaggcccgt
     2581 agac
//
LOCUS       DASTUV010000192         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_593752, whole genome shotgun sequence.
ACCESSION   DASTUV010000192 DASTUV010000000
VERSION     DASTUV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593752"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..970)
                     /locus_tag="VFT03_03910"
     CDS             complement(161..970)
                     /locus_tag="VFT03_03910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4847318.1"
                     /translation="MRNAEKPGLFLPAGALQEARDRAEGCLDLCLDLLFARPPAYETV
                     NWEDALRDLGAHLGRVEDLLGEPALAEVEENTRRLLEEIRHKDPFSPRWAADSLLARC
                     SYLVCRLLEPQVVAETGVAYGVSSAFILRALEENGHGILHSVDLPPLRKRYDEFWGIA
                     VDGTLRSRWSIHRGSSKRVLPELVEEYGVVDLFLHDSLHTYRNMRRELEVVWPRLRAG
                     GVILADDVERNHAFGDLRRKNPALWRVVRDRQERPLHAGAIPRATFGIVIK"
     gene            1073..1675
                     /locus_tag="VFT03_03915"
     CDS             1073..1675
                     /locus_tag="VFT03_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEU4847319.1"
                     /translation="MKWLKVYGLPRLPLFLARLAMRLGLPGPTWYKWKAASAGTGVIL
                     DEMIRAADDLGYDGQKIGKLAMTRIGEKQGTDLREQLGVKTMKDAVDVVMLANRFFDI
                     EITLTEQGDGTYVINADRCPWFGGMGHDGVPGWDVKPCAAFSTYEKAMVQAINPNVKL
                     SYTEKRTTGGHTCRGVYQYRDPELGDNVEPEFVSIGRKPK"
     gene            1678..2277
                     /locus_tag="VFT03_03920"
     CDS             1678..2277
                     /locus_tag="VFT03_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEU4847320.1"
                     /translation="MQRIEIDTARGKERVPPGQYLVGDRWPILTYGATPAFDPESWDF
                     TVSGLVENPLSYTWEEWNDLPTVEVKADMHCVTSWSKLDNVWRGVPAKTILEMADPKP
                     DARFFSVFCDGGYTTNAPLEELFEDDALFATHHNGEPLEPGHGYPMRLVIPRLYAWKS
                     AKWARGIELLAEDKPGFWENNGYHNYGDPWQEQRYSFEF"
BASE COUNT          447 a          761 c          771 g          401 t
ORIGIN      
        1 gcaggctcgc tctacggcgt caatgccttc gaccagcccg gcgtcgaggc gggaaagcag
       61 atcacctaca aggggatggg ccgacccgga tactaggcat cagggattgc cgcgcgtcga
      121 ggtcgagtgg cggttgctcc acgtgtccct tacctgcagc ctacttgatc acgatgccga
      181 aggtcgctcg cggtatggct cctgcgtgga gcgggcgctc ctggcggtcc cggacgacgc
      241 gccagagcgc gggatttttt cgccgcagat cgccgaaggc gtggttcctc tcgacgtcgt
      301 cggcgaggat cacgcctccg gctcgcaagc ggggccagac aacctctaac tctcgccgca
      361 tgttgcggta ggtgtgcagg ctgtcgtgca ggaagaggtc cacgacgcca tactcctcga
      421 ctagctccgg caggacccgc ttgctggatc cgcggtggat gctccatcgg gatctcagcg
      481 tcccgtccac ggcgatgccc cagaactcgt cgtacctctt tctcaagggc ggcagatcca
      541 cgctgtgaag tattccgtga ccgttctctt cgagggctct gaggatgaac gcagagctca
      601 ccccgtaggc caccccggtc tcggcgacga cctgcggttc gaggaggcgg cacacaaggt
      661 aagagcagcg cgccaggagc gagtcggcgg cccagcgggg gctgaaaggg tctttgtgcc
      721 ggatctcctc cagcagccgg cgcgtattct cttcgacctc ggcaagcgcc ggttctccga
      781 gtaagtcctc cacacgcccc aggtgagccc cgaggtcacg gagcgcgtct tcccaattca
      841 cggtctcgta ggctggcggt ctcgcgaaga gaaggtccag gcagagatcc aggcaccctt
      901 cagcccggtc acgggcctcc tgtagtgccc cagcggggag gaagagtccg ggtttctcgg
      961 cattgcgcac ggcggcacaa tggtaatcca gcggaggacc acgcggtagt cggaggcacg
     1021 agggttataa tagggtactt gctggcgtaa gcgcaggaag gaagttcgaa ttatgaagtg
     1081 gctcaaggtc tacggtctgc cccggctccc gctcttcctc gctcgtctgg cgatgcggct
     1141 cgggctgccg ggcccgacgt ggtacaagtg gaaggcggcg agcgccggga caggggtcat
     1201 cctggacgag atgatccggg ccgcagacga cctcggctac gacggccaga agatcggcaa
     1261 gctcgctatg acccgtatcg gcgagaagca gggcaccgat ctcagggagc agctcggcgt
     1321 gaagacgatg aaggacgccg tggacgtggt catgctcgcc aaccgcttct tcgacatcga
     1381 gatcacgctc accgagcaag gcgacggcac ctacgtcatt aacgccgacc gctgcccctg
     1441 gttcggcggg atgggccacg acggcgtccc cggctgggac gtgaagccct gcgccgcctt
     1501 ctcgacctac gagaaggcga tggtccaggc cattaacccg aacgtcaagc tctcctacac
     1561 cgagaagcgc accacaggcg gccatacctg ccgcggcgtc taccagtacc gggaccccga
     1621 gctcggcgac aacgtcgagc ccgagttcgt gagcatcggc aggaagccca agtaaacgtg
     1681 cagcgcatcg agatagacac ggcgcgcggc aaggaaaggg tgccgccggg ccagtacctc
     1741 gtcggcgacc gctggccgat cctgacctac ggcgcgacgc ctgccttcga ccccgagtcc
     1801 tgggacttca cggtctcagg gctcgtcgag aaccctttga gctacacctg ggaggagtgg
     1861 aacgatctgc ccaccgtcga ggtcaaggcg gacatgcact gcgtgacctc ctggagcaag
     1921 ctggacaacg tgtggagggg cgtgccggca aagacaatac tggagatggc cgacccgaag
     1981 ccggatgccc ggttcttctc ggtcttctgc gacggcgggt acacgacgaa cgctcccctc
     2041 gaagagctct tcgaggatga tgctctcttc gccacccacc acaacggcga gccgctcgag
     2101 cctggccacg gctacccgat gcgcctcgtg ataccgcgcc tctacgcctg gaagagcgcc
     2161 aagtgggctc ggggcataga acttctcgcc gaggacaagc cgggcttctg ggagaacaac
     2221 ggctaccaca actacggcga cccctggcag gagcagcgct acagcttcga gttttaagca
     2281 cgctagggct tcgccgtcgc ttgtcagcac gcctccctgc ggtcggcttg tcagtatttc
     2341 agcttctctt ggtgctaaga gaagctgaga agccgcctgg
//
LOCUS       DASTUV010000193         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_937177, whole genome shotgun sequence.
ACCESSION   DASTUV010000193 DASTUV010000000
VERSION     DASTUV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937177"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFT03_03925"
     CDS             complement(<1..431)
                     /locus_tag="VFT03_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847321.1"
                     /translation="MTTRGTGPADTLEPSPGRGSKMLGGRAVLVSSVSTVLFFAVIAA
                     IVVLAPGSEVVAERFFDPFHLRQSFLGTESEPSVARAFLLNVYMFTISEVFILVLALV
                     IAVVRQIPGPVFFPFRMVAVAYTDLFRGIPLILVLYIIGFGV"
     gene            complement(433..1257)
                     /locus_tag="VFT03_03930"
     CDS             complement(433..1257)
                     /locus_tag="VFT03_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4847322.1"
                     /translation="MIGFLVVTLLLAGCGQQGSGGSAQGGSGNKESCDIKNPPLFKEG
                     VLTVATDKPAYPPWFEGDPEHYSGYEGEVANEVAKRMDLPIEWVVEPFNKSYAPGAKD
                     FDFDINQITITPEREQVVDFSEGYFDNAQGVLVLKDSPVADAQSISDLKDAKLGAQVG
                     TTSLDFINETIQPNEEPKVYDTTNDAKSALESGQIDAIVTDLVTTVYLRDFEIKNTKV
                     VGQYPRNEQFGMLFEQGNPLVGCVNEVLGEMKEDGTLQNLKEEYLQQYLSVPTLKE"
     gene            1466..2047
                     /locus_tag="VFT03_03935"
     CDS             1466..2047
                     /locus_tag="VFT03_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4847323.1"
                     /translation="MGYSSGRAVASEDAKLAERAKNGDTAAYEKLVRGHQAVAFRTAY
                     LITGDASEAEDAAQEAFVKAYRALGGFRTGAPFRPWLLAVVANEARNRRRAAGRRLGL
                     ALRVAGESPAGDTASPETAALAAERRAELLGTLGALREEDRMVIGYRYFLQLSEAEMA
                     AALGCAPGTIKSRLSRALARLRERMMEDEDAAG"
     gene            2034..>2441
                     /locus_tag="VFT03_03940"
     CDS             2034..>2441
                     /locus_tag="VFT03_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847324.1"
                     /translation="MQPDRDLERELRELGSRVEYPPTPDLARAVRSRLEAGEQPVPRR
                     GWFDLPVVRWTAVAAVVLLAAFPVLSPAMRDTVAGWFESGQVSSSGQAADGAREAAPA
                     GGDVTLKGKGGSHAAEAARPSSVGQPMSSSGAGG"
BASE COUNT          424 a          771 c          815 g          431 t
ORIGIN      
        1 accccgaacc ctatgatgta gagcactagg atcaggggta tcccgcggaa gaggtcggta
       61 tacgcgacgg cgaccatccg gaaagggaag aagacgggac ccggtatctg gcgcaccacg
      121 gcgatcacca gcgcgagcac caggataaag acctccgaga tagtgaacat gtagacgttc
      181 aggaggaagg cccgggccac ggaaggctcg ctctccgtcc cgaggaacga ctgcctgagg
      241 tggaaggggt cgaagaaccg ctccgcgact acctcgctgc ccggcgcgag cacgacgata
      301 gcggcgatga ccgcgaagaa caggaccgtg ctcacgctcg agacgaggac cgcgcggccg
      361 ccaagcatct tcgagccgcg ccccggggac ggctcgagcg tgtccgcggg gccggtgccc
      421 cttgtcgtca tcctactcct tgagggtcgg gacgctgagg tactgttgca ggtactcctc
      481 cttgaggttc tgcagcgtcc cgtcctcctt catctcgccg aggacctcat tcacgcagcc
      541 caccagcggg ttgccctgtt cgaagagcat cccgaactgc tcgttcctcg ggtactggcc
      601 cacaaccttc gtgttcttta tctcgaagtc gcgcaggtac accgtcgtca ccaggtcggt
      661 gactatggcg tcgatctggc cgctctccag agccgacttg gcgtcgttgg tggtgtcgta
      721 gaccttgggc tcctcgttcg gctgtatcgt ctcgtttatg aaatccaggc tcgttgtccc
      781 cacctgcgcg ccgagcttcg cgtccttgag gtcggagatg gactgcgcgt cggcgacggg
      841 agaatctttg agcacaagca cgccctgagc gttgtcgaag tagccctccg agaagtcgac
      901 gacttgctct cgctccggag ttatggtgat ctgatttatg tcgaagtcga agtccttggc
      961 gccgggagcg taggacttgt tgaacggctc gacgacccac tctatcggga gatccatcct
     1021 ctttgcgacc tcgttcgcga cttctccctc gtagcccgag tagtgctcag ggtcaccctc
     1081 gaaccacggc gggtaggctg gcttgtccgt cgcaacggtc agcaccccct ccttgaagag
     1141 cggcgggttc tttatgtcgc aggactcctt gttccccgag cctccctgcg ccgaccctcc
     1201 cgagccctgt tgcccgcatc cggccagcaa cagagtcacg accagaaaac cgatcaatat
     1261 agttcgtttc atagcgcccc tgactccgat cctccgccgg gaagagcctg tatcccggcg
     1321 aatatgccca gaacttctca tacggtgcca agtataggga cggccaaaaa gccttgcaat
     1381 ttcgccgggg tctctcgcgc cgggctctca gaagatccat catgggccac gggaaccttt
     1441 cggcgccctg cgttgtataa gggtagtggg ctactctagc gggagggctg tcgcctcgga
     1501 ggacgcgaag ctcgccgagc gggcgaagaa cggggatacg gccgcgtacg agaagctggt
     1561 gcgcggtcac caggcggtcg ccttcaggac cgcctatctc atcacgggcg acgcctcgga
     1621 ggcggaagac gccgcacagg aggctttcgt caaagcgtac cgcgccctgg gaggcttccg
     1681 gaccggtgcc cccttcaggc cctggttgct cgcggtggtc gccaacgagg ccaggaaccg
     1741 ccgcagggct gcgggccggc gcctcggcct cgccctgcga gttgctggag agagcccggc
     1801 tggggacact gcctcccccg agaccgctgc cttggcggcg gagcgtcggg ccgagctgct
     1861 agggactctg ggggcgctgc gcgaggagga ccggatggtg atcggctacc gctacttctt
     1921 gcaactctcc gaagcggaga tggccgcggc ccttggctgc gcgccgggaa caatcaagtc
     1981 gcggctctcc cgggcgctgg ccaggctgcg cgagaggatg atggaggacg aagatgcagc
     2041 cggataggga tctcgaaaga gagctcaggg agctcgggtc ccgcgtcgag tacccgccga
     2101 ccccggacct ggcgcgggca gtacgcagcc gcctcgaagc aggcgagcaa ccggtgccgc
     2161 gccgggggtg gtttgatctt ccggtagtga gatggacggc cgtggctgcg gtcgtgctgc
     2221 tcgccgcttt ccctgttctc tctccggcga tgcgcgacac ggtcgcaggg tggttcgaga
     2281 gcgggcaagt ctcgagcagc ggtcaggccg cggatggagc cagggaggct gcgcccgccg
     2341 gaggcgatgt cacgcttaag ggcaaaggcg gtagtcacgc cgcggaagcg gcgcgaccga
     2401 gcagcgtcgg gcagcccatg tcgagctctg gcgcgggagg g
//
LOCUS       DASTUV010000194         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_849141, whole genome shotgun sequence.
ACCESSION   DASTUV010000194 DASTUV010000000
VERSION     DASTUV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849141"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /gene="alr"
                     /locus_tag="VFT03_03945"
     CDS             <1..872
                     /gene="alr"
                     /locus_tag="VFT03_03945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU4847325.1"
                     /translation="IKAPILVFTDLLPDRLPLAEELRLTVTAHSLASARRVAARPGLE
                     AHLKVNTGMNRWGVEPHQVGEARKILDSQLSGVYTHLASADCDEVATRCQLSLFDAVL
                     AAHPFGGALVHAANSAGTLWHPDARYDCVRPGVALYGLHPAGDEGDPSSEGLRPAMVL
                     KSYVADTRRLEAGDGVSYGLTFTAGSTTFAATVPVGYAEGYRRALSGRAEALIRGERR
                     PLLGRVTMDACVFEVDGTVEVGDEVVLLGEQAGGRVTAEEIGAWAGTINYEVTTGVNP
                     RRVERSYEDVRGP"
     gene            856..1200
                     /locus_tag="VFT03_03950"
     CDS             856..1200
                     /locus_tag="VFT03_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847326.1"
                     /translation="MFEDLNWKYAARRAAVVIAIYLGLLYVLSIAFPESGFKLETREQ
                     LLSLLVNAVMFFFVFTLVYALVDRSRRRRLGETTQGSKADKPTGEAEGPSGPLKGRPN
                     PNTSRKKARRKR"
     gene            1204..1959
                     /locus_tag="VFT03_03955"
     CDS             1204..1959
                     /locus_tag="VFT03_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4847327.1"
                     /translation="MDLREALGPPLDSGRLLDAVPELSLSRGVEQSPYHHLDTFEHTL
                     EVVRGVERELEEARLGARVAQHRVEGLRLAAALHDVAKPVTRGEVEGRVLFVSHDSLG
                     ARLVRRICRRLGLSASLTDLAATLTALHLKIGFMESDRTDYTPGRLARAAGPFGEELA
                     VLSWADRLAAQGPRLKPEHVERHQALCARFLKTSSALGPHLEPDYGALAGRLAPGDPA
                     QPADVGYAASRLRLLASRGTGGERAFAQVPRLL"
     gene            2028..2228
                     /locus_tag="VFT03_03960"
     CDS             2028..2228
                     /locus_tag="VFT03_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU4847328.1"
                     /translation="MRIKRAKRDRWILGVCGGIAHTYGWSSNLVRLAAVVLAIIIPGF
                     SVIPVIAIYVLLGAIMPESEEY"
     gene            2232..2861
                     /locus_tag="VFT03_03965"
     CDS             2232..2861
                     /locus_tag="VFT03_03965"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4847329.1"
                     /translation="MEFAEAQKEALGCTRCGLCHSRTQVVFGEGPLNAELFVVGEAPG
                     FNEDREGRPFRGASGMLLERLLSSAGLERERVYMTTLVKCRPPGSPPRSPKPSEVSSC
                     RPYLVAQLAAVDPKVVVAMGDLASRLLTGRKEAVARIRGRAIPLDGRYVFPTLSPSLA
                     LYTPADRALLISDFSRLPELLEADRPSTYETLNVPHAPAEREPLQPGLW"
BASE COUNT          449 a         1018 c          996 g          453 t
ORIGIN      
        1 ggatcaaggc cccgatactg gtcttcaccg atctcctgcc ggacaggctg cccctcgcgg
       61 aggagcttag actcaccgtc accgcgcact cgctcgcgag cgcgcgccgc gtcgccgccc
      121 gccccggcct cgaggcgcac ctgaaggtaa acacggggat gaaccgctgg ggcgtcgagc
      181 ctcaccaggt gggggaggcg cgcaagatcc tggactccca gctttcgggc gtgtatacgc
      241 acctcgcctc ggcggactgc gatgaggtcg cgacgcggtg ccagctctcc ctcttcgacg
      301 ctgtgctggc cgcccaccct ttcggcggcg cgctggtgca tgctgcgaac tccgccggca
      361 ccctctggca ccccgatgcg cgctacgact gcgtgcgtcc cggcgtggcc ctctacggcc
      421 tgcaccctgc aggcgacgag ggagaccctt cctccgaagg gctgaggcct gcaatggtcc
      481 tcaagagcta cgtcgccgac acgcggcggc tggaggcggg cgatggagta tcctacgggt
      541 tgaccttcac ggccggctcg acgacgttcg cggctacggt gcctgtaggg tacgccgagg
      601 ggtacaggcg ggcgctctcg gggcgtgccg aggccctgat caggggcgag cggcgtcccc
      661 ttttgggacg ggtcacgatg gacgcctgcg tcttcgaggt ggacgggaca gtggaggtcg
      721 gggacgaggt cgtgctcctc ggcgagcagg ccggcgggcg ggtgacggcg gaggagatcg
      781 gcgcgtgggc cgggacgata aactacgagg taacgaccgg ggtcaacccc cggcgcgtcg
      841 agaggagcta cgaggatgtt cgaggacctt aactggaagt acgccgcccg gcgcgcggcg
      901 gtggtcatcg cgatttacct gggcctgctc tacgtcttga gcatcgcttt cccggagagc
      961 ggcttcaagc tggagacccg cgagcagctc ctgagcctcc tcgtgaacgc tgtcatgttc
     1021 ttcttcgtct ttactctggt ctacgccttg gttgaccgca gccgccgccg gcgcctggga
     1081 gagacgaccc aggggagcaa ggcggacaag cccaccggcg aggccgaagg gccgtccgga
     1141 ccgctgaagg ggcgacccaa cccgaacacg agccgcaaga aggcccgccg gaagcgctag
     1201 cttttggacc tccgcgaggc gctcgggccg ccgctcgact ccggaaggct actggacgcc
     1261 gtccccgagc tctccctctc gaggggcgtc gaacagagcc cttaccacca cctcgacacc
     1321 ttcgagcaca ccctcgaggt cgtgcggggc gtcgagcgcg agctggagga agcccggctg
     1381 ggggcccgcg tcgcccagca ccgcgtcgag gggctccgtc tcgccgccgc tctccacgac
     1441 gtcgccaagc ccgtaacgcg cggcgaggtc gagggcaggg tgctcttcgt ctcccacgac
     1501 tctctgggcg ccaggctcgt tcgcaggatc tgccgccggc tcgggctctc cgcctccctc
     1561 acggaccttg cggcgacgct taccgcgctc cacctgaaga tagggttcat ggagagcgat
     1621 cgcacggact acaccccggg gaggctcgcc cgggccgcag gtcccttcgg ggaggagctc
     1681 gctgtcctga gctgggccga ccgcctcgca gcccagggcc cgcgcctcaa gcccgagcac
     1741 gtcgagcgcc accaggctct atgcgcccgg ttcctgaaga cgagcagtgc gctcggcccc
     1801 caccttgagc ccgactacgg ggcgctcgcc gggagattgg cacctggtga cccggctcaa
     1861 cccgcggacg tcggttacgc cgcgagccgt ctgcggctcc tcgcgtcccg cgggacgggc
     1921 ggggagaggg cgtttgcgca ggtcccgcgg ctgctctgaa gccggcatcc tggtagaatc
     1981 gcggccatcg gcaaaccggc tccgtagagg ccgggggagg caaggatttg cgcataaaac
     2041 gggcgaagag ggaccgttgg atcctgggcg tctgcggcgg catagcccac acttacgggt
     2101 ggagctccaa tttggtgcgg ctcgcagccg ttgtgctcgc gatcatcatc cccgggttca
     2161 gcgtgatacc ggtgatcgcg atctacgtcc tgctcggcgc catcatgccg gagagcgagg
     2221 agtactagcg cttggagttc gcggaggccc agaaggaggc tctaggctgc accagatgcg
     2281 gcctctgtca ctcgaggact caggtcgtct tcggcgaagg cccattgaac gccgagctgt
     2341 tcgtcgtggg ggaggctcca ggcttcaacg aggacaggga gggcagacca ttccgcgggg
     2401 cctcgggaat gctcctcgag cggctgctct cctcggccgg gctcgagcgc gagagggtct
     2461 acatgaccac cctcgtcaag tgccgcccgc cagggtcgcc gccccgctcg ccgaaaccct
     2521 cggaggtgag ctcctgccgg ccttacctcg tcgcccagct cgcggcggtg gacccgaagg
     2581 tcgtggtagc catgggggac ctggcgagcc gcctcctcac cgggaggaag gaagcggtcg
     2641 cgcgcatacg aggccgggcc attcccctgg acggacgcta cgtcttccct acgctctccc
     2701 catcgctcgc gctctacacc ccggcggaca gggcgctcct gatctcggac ttctcccgcc
     2761 tccccgagct tctcgaggcc gaccgcccct cgacctacga gaccctgaac gtcccgcacg
     2821 cccccgccga gcgcgaaccc ctccaacccg gcctctggtg acgactccgg aagctagagc
     2881 aaccggcttc tcgggacacg ctcaaggtat agagac
//
LOCUS       DASTUV010000195         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1508778, whole genome shotgun sequence.
ACCESSION   DASTUV010000195 DASTUV010000000
VERSION     DASTUV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508778"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFT03_03970"
     CDS             complement(<1..878)
                     /locus_tag="VFT03_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4847330.1"
                     /translation="MPEIEAIMILLAAVAALATLANRIKIPYPILLVLGGLALGFVPY
                     LVPAFPRIELEPEVVFFLFLPPLLYVSAIFTSWRDFRANLRPITLLAVGLVLMTTCVV
                     AAVAHWALGFSWAAAFVLGAIVSPTDAVAATSIVQRLGVPRRIVTVLEGESLVNDATG
                     IVLYRIAVAAVVFGGFSLWQAGLQFVIGAIGGISVGLAVGWLVIWARRHAADDPDVQN
                     TISLLTPFTAYLLAEEPSHWAWEQFHLPGEFAFSGVLAVVTTGLYLGRRGPELISPEV
                     RLQGYAFWELVTFLLNG"
     gene            complement(916..1515)
                     /locus_tag="VFT03_03975"
     CDS             complement(916..1515)
                     /locus_tag="VFT03_03975"
                     /inference="COORDINATES: protein motif:HMM:NF023070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEU4847331.1"
                     /translation="MFARATPRCTHLDEIRDVTPSAQGCEECLQMGGTWVHLRECLLC
                     GHVGCCDQSPNKHATRHFHATGHPLIQSFQPGEDWIWCYVDQVIIEPRWGGDRVPTMI
                     VLANLKDGVDPEDYERWILESYAPAVRDLPSVEDWRDYRVSGLLGSEAAPPYRYVVTL
                     EVSDMEQLGRDMSGEEMQRHFSELHDLAEVTQIMAERFA"
     gene            complement(1530..>2298)
                     /locus_tag="VFT03_03980"
     CDS             complement(1530..>2298)
                     /locus_tag="VFT03_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847332.1"
                     /translation="GANLLTLFLYAALGGSLYYLPFNLIQVHGYSATAAGSAFLPFVL
                     ITFVLSRWAGGLVTRYGAKMPLVIGPTIAAAGFVLFALPGTEGSYWTTFFPAVVVMGL
                     GMALVIAPLTTTALTAVSGRHAGLASGVNNAVSRTASLLCIPVLGIFVFMTFSTQLDA
                     GTKELDLPQEARQQLEAEKVDLAGAEVPEGLNGSTATAVQRAIDEAFVAGYRVAMLIA
                     AGLAVVSAVVASIMIEGKGRAVESREPGSAGGETAPA"
BASE COUNT          431 a          759 c          738 g          370 t
ORIGIN      
        1 ccgttcagga gaaatgtaac gagctcccag aacgcatagc cctgcaggcg cacctccggg
       61 gagatcagct ccgggccgcg ccggcccagg tagagccccg tcgtcaccac agcgagcacc
      121 cccgagaacg cgaactcccc ggggaggtgg aactgctccc acgcccagtg cgacggctcc
      181 tccgccagca ggtaggccgt gaacggcgtg agcagagaga tcgtgttctg tacgtcaggg
      241 tcgtcggcgg cgtgcctgcg cgcccagatg accagccagc ccaccgcgag ccccacggag
      301 ataccgccga tggcgcctat gacgaactgc agtcccgcct gccacagcga gaaccccccg
      361 aacacgactg cggccactgc tatccggtag aggacgatgc ccgtggcgtc gttgacgagg
      421 ctctctcctt cgagcactgt cactatgcgc cgcgggacgc cgaggcgctg gacgatggag
      481 gtcgcggcta cggcgtcggt aggggacact atggcgccga gtacgaaggc tgccgcccag
      541 gagaacccga gagcccagtg agcgacagcg gcgaccacgc aggtggtcat gagcacgagg
      601 ccaaccgcga ggagcgtgat cggccgcagg ttcgctctga agtcacgcca ggaagtgaag
      661 atcgccgaga catagagaag cggcggcaag aagaggaaga acacgacctc aggctcgagc
      721 tctatccgcg gaaacgccgg tacgaggtag ggcacgaagc cgagcgcgag cccgcccagg
      781 acgagaagga tcggataggg gattttgatc ctgttggcca gggtcgcgag cgccgcgaca
      841 gcagcgagca ggatcatgat cgcttctatc tcaggcaacg cccgtatcct cttcgtccca
      901 ggcggagctc atcggtcagg cgaaccgttc ggccatgatc tgggtgacct cggccaggtc
      961 gtgcagctcc gagaagtgcc tctgcatctc ctcgcccgac atgtcgcgcc cgagctgttc
     1021 catgtcgctt acctcgaggg tcacgacgta ccggtaggga ggtgcggctt cagagccgag
     1081 caggccgctc accctgtagt cccgccagtc ttcgaccgat gggagatctc ttacggccgg
     1141 cgcgtaagac tccaggatcc agcgttcgta gtcctcgggg tccacgccat ccttgaggtt
     1201 ggccagaact atcatcgtcg gcactctgtc acctccccaa cgcggctcta tgatcacctg
     1261 gtcgacgtaa caccagatcc aatcctcccc cggttggaaa gactggatca gcgggtgtcc
     1321 tgtggcgtgg aagtgcctgg tcgcgtgttt gtttggagat tggtcgcaac atcccacgtg
     1381 gccgcagagt agacactcgc gcaggtgtac ccaggtgccg cccatctgca ggcactcctc
     1441 gcagccctgg gcgctcgggg tgacgtccct gatctcatcc aggtgtgtgc accgtggcgt
     1501 ggctctcgcg aacatggatc actccttttt caagctggcg ccgtctcgcc ccctgcgctt
     1561 cctggctccc gggactctac ggcgcgtcct ttgccttcga tcatgatgct ggccaccacc
     1621 gcgctcacga ccgcgagtcc cgcggcgatg agcatggcga cacggtagcc ggcgacgaaa
     1681 gcctcgtcta tagccctctg caccgcggtc gccgtactac cgttgagtcc ctccggcacc
     1741 tcggcccccg cgaggtctac cttctcggcc tcgagctgct ggcgcgcctc ctgcggcagg
     1801 tccagctcct tcgttccggc gtccagttgg gtcgagaacg tcatgaacac gaagatgccg
     1861 agcacaggaa tgcagagcag gctcgccgtc cgggagacgg cgttgttgac gccggaggca
     1921 agtccggcgt gcctgccgga gacggcggtc agggccgtcg tcgtcagcgg cgctatgacg
     1981 agcgccatcc ccaggcccat caccacgacc gcggggaaga acgtcgtcca gtaagatcct
     2041 tcggtcccgg gcagagcgaa caggacgaag cccgccgcgg ctatggtagg tccgatcacg
     2101 agcggcatct tggcgccgta gcgggtgacg agaccgccgg cccaccgcga taacacgaag
     2161 gtgattagca cgaagggcag gaatgcgctg ccggccgccg tcgccgagta accgtggacc
     2221 tgtatgaggt tgaacggcag gtagtacagt gacccgccca acgccgcata caggaacagc
     2281 gtcaggaggt tagccccc
//
LOCUS       DASTUV010000196         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_213371, whole genome shotgun sequence.
ACCESSION   DASTUV010000196 DASTUV010000000
VERSION     DASTUV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213371"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFT03_03985"
     CDS             complement(<1..285)
                     /locus_tag="VFT03_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847333.1"
                     /translation="MARSWRSFFKRDREEPAESGVATRTEDPDSNGSAPSGSDPSGAE
                     VQAAEGVLAERREPEVVEAPVEEAEPGEVEEALPEPGVVEEAPDAEAAAET"
     gene            complement(297..>2570)
                     /locus_tag="VFT03_03990"
     CDS             complement(297..>2570)
                     /locus_tag="VFT03_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847334.1"
                     /translation="RIGDLEAEHGTRSEETARRKRAATQARQRAAEAEKQRSRLAADL
                     EVLHGRLAHIAPEEGDRGALPDDELGSLASVADSLERSLEGDLASRLGGLRERLGDRL
                     SLVEAHAEEADRRRGALAAAIGRAESRVRALRQSAPGGAGTRLYEVIRARPGYEAAVE
                     AALGDFGAGVLARDVDEGMRILSSTERVAVRLDAVRVEEGEIPPGKPLVDCVEVVDER
                     YAEAVERLLGGIYVTEEPRANAPANGYVTVTREGLRLTRTSVSLRPGDAGFAREARLA
                     REAERLDELEKGPGEMLYALRESVSDASRRIEGRAARAQSISTLAERSLRVRALLARE
                     TRRRCAAAEQFRRDLLARRELRETLSAEALEKQESLLAAEAASAAAESEVAAATRAAS
                     AADAAAEESGRRLKEARAALSEGMDRRDRISRRLAHAREISGRDTHRAAQVARRAAHA
                     SGLLTNSVRERHNALRAVRSRTSDALRRSSAEQTDLARKAAALAGDLAAARSVVARLT
                     EDLLRPRAQAAEAADEMESEWGATLEAARREAERHPPDTAEERGRLARKLKRFGDVNL
                     LALSQEQELRERHQLIAAQRADADAAATELNRIILSIDREIEARFSETFGRVREAFGE
                     MVPRMMEGASGVLELSEEGVEVGLRLGRRGWKPLRVLSGGERALLALSFLFSVFLSRP
                     GGDSGAFCVLDEAEAALDDLNLARFLAVVDSHRAGGQYLLVTHQKRTMAAADVLYGVV
                     QDAAGATTVVSKRMQGE"
BASE COUNT          338 a          968 c          843 g          421 t
ORIGIN      
        1 tgtctccgcc gcggcctcgg catccggcgc ttcctcgacc acacccggtt ctgggagcgc
       61 ctcctcgacc tcgccgggct cagcctcctc gaccggagcc tcgacgacct cgggctccct
      121 ccgctcggcg agcacccctt cagccgcctg cacctcggcg ccggagggat cactcccgga
      181 gggagcactc ccgttagagt ccgggtcctc ggtccgcgtc gctacgccgg actccgccgg
      241 ctcttctctg tcccgcttga aaaaactacg ccacgagcgc gccatgaact acttctctac
      301 tctccctgca tgcgcttcga tacaacagtg gtggccccgg cggcgtcctg gacgacgcca
      361 tagagcacgt ccgccgcggc catcgtccgc ttctggtggg tcaccagcag atactggccg
      421 ccggccctgt gagagtctac cacagcaagg aaacgcgcca ggttgagatc gtcgagcgca
      481 gcctccgcct cgtcgagcac acagaacgcc cccgaatccc cgccaggacg gctcaggaag
      541 acgctgaaga ggaacgaaag cgcgagcagc gcccgctccc ccccggataa cacccgcaac
      601 ggcttccacc cccgccggcc gagccgcagc ccgacctcca cgccttcctc agagagctcc
      661 agcacccccg aagcaccctc catcatccgc gggaccatct ccccaaacgc ttcccgaacc
      721 cgcccgaacg tctccgagaa ccgcgcctcg atctcccggt ctattgagag tattatgcgg
      781 ttaagttcgg tagctgcggc gtcggcgtcg gcgcgctggg ctgcgatgag ctggtggcgc
      841 tcgcgcagct cttgctcctg ggagagggcg aggaggttga cgtcgccgaa acgcttgagt
      901 ttgcgcgcca ggcggccccg ctcttcggcg gtgtcgggcg ggtggcgctc ggcttcgcgc
      961 cgggcagcct cgagggtggc gccccattcg ctctccatct cgtcggcggc ttcggcggcc
     1021 tgggccctgg ggcggagcag gtcttccgtg aggcgtgcga cgaccgagcg ggcggctgcg
     1081 aggtcgcccg cgagggccgc tgccttccgg gccaggtcgg tctgctcggc ggaggagcgg
     1141 cggagggcgt ctgaggtccg ggatctcaca gcgcgtagcg cgttgtggcg ttcgcggacg
     1201 gaattggtga gcagtcctga ggcgtgcgcc gcgcgccggg cgacctgcgc tgcgcgatgg
     1261 gtgtcccggc cggagatctc cctcgcgtgg gcgagccggc gggagatccg gtccctgcgg
     1321 tccatgcctt cggagagagc ggctcgcgct tctttcagcc tccggcccga ctcctcagcg
     1381 gcggcgtcgg ctgcggaggc cgcccgtgtg gccgctgcta cctcgctctc cgcggcggcc
     1441 gaggccgctt cggcagctag gaggctctcc tgcttctcga gcgcctctgc ggagagggtt
     1501 tcccgcaact cccgccgcgc gaggagatcc ctccggaact gttcggctgc cgcgcaacga
     1561 cgccgggtct cgcgagctag cagggcacgg acacgcaggg agcgctctgc gagcgtggat
     1621 atactctgcg ccctggccgc gcgcccctcg atgcgccggg aagcgtccga gacgctctcc
     1681 cgcagagcgt agagcatctc gcctggtcct ttctcgagct cgtccagtcg ctcggcctcg
     1741 cgcgccaggc gagcctcgcg tgcgaaaccg gcgtcccctg gcctgaggct gacgctcgtg
     1801 cgcgtcaggc gcaggccctc tcgggttacg gtcacgtaac cgtttgccgg ggcgttagcc
     1861 ctgggctcct cggtgacgta gatgccgccg aggagccttt ccacggcctc ggcgtagcgt
     1921 tcatcgacga cctccacaca gtcgacgagg ggcttgccgg gtgggatctc gccctcttcg
     1981 acccttacgg cgtcgaggcg gacggctacg cgctcggtgc tggagaggat cctcatgccc
     2041 tcgtcgacgt cccgggcgag aacgcccgcg ccgaagtcgc cgagggcggc ttcgacagcc
     2101 gcctcgtaac cggggcgagc ccggatcacc tcgtacagcc tggtccctgc gccgcctggc
     2161 gcggactgac gcaacgccct gaccctcgac tccgcgcggc cgatcgccgc cgccagcgcg
     2221 ccgcggcgcc tgtctgcctc ctcggcgtgc gcctccacga gcgagaggcg atcaccaagc
     2281 cgctctcgca atcccccaag cctcgaggcg aggtctcctt ccaacgagcg ctcgaggcta
     2341 tccgccacac tcgcaaggct cccgagctca tcgtccggca aggcgccgcg atcgccttct
     2401 tccggcgcga tgtgggcgag ccgcccatga aggacctcca ggtcggcggc gagcctggat
     2461 ctctgcttct cggcttcagc cgctcgctgc cgggcctgcg tcgcggcccg ttttcgcctg
     2521 gccgtctcct cgctcctggt cccgtgttcc gcctccaggt ccccgatccg
//
LOCUS       DASTUV010000197         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_562585, whole genome shotgun sequence.
ACCESSION   DASTUV010000197 DASTUV010000000
VERSION     DASTUV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562585"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="VFT03_03995"
     CDS             <1..1920
                     /locus_tag="VFT03_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847335.1"
                     /translation="SAATEPVTIELDPLSSEESDRLIQNLLGPSAISKGYRARVADAA
                     EGNPLFVEEMLGMLIDDERLRREEGMWVATDEDVSVPTPPSIQSLLAARIERLPQAER
                     EILQRASVAGKVFWWGAVADLSPSDDRSGVGSRLQTLVRRGMIRPDLSEFAGQDAFRF
                     KHILIRDAAYESVPRELRAGLHERLANWIERTAGERGDEYEEILGYHLEQACRQRLAS
                     AGERDRELAARASSYLASAGDRALDRGDARAAVNLLSRASKLSAAERPEDLAVRLSLG
                     RALHYSGEFRQALGVLAELAKQAIAAGDQRLEWRARIQQQQIIVATTTDVSFEEIRTT
                     ADRAIEIFTELDDDLGLFWSWELMAWMHFNAGRAHEAQKAASKATSYAPSPAELADGL
                     LDGLTYAVFGPMPVEEALRLCGDVRGHVEGDPAREASLLQLEAILEAMRGNIEKARAG
                     ISRARSTWQELGHVTALASFTESASDVEWYAGDVIGAERERRSGYEAWRKMRVVGYQS
                     TQAALLAELLVELGRDDEALSLTRESEKLAADYDITAQVPWRGARARVLARRGEVEEA
                     VRLAREAEAMAERTDWLKLQGDTQIDLADVLRLAGRSEEATHAALKAAELYEQKGDIV
                     ATARAGAVLVGLKHK"
     gene            2183..2743
                     /locus_tag="VFT03_04000"
     CDS             2183..2743
                     /locus_tag="VFT03_04000"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847336.1"
                     /translation="MASAMGRYLIRRTLFMILVLFVVSLITFIIFVKLPASDPARRAA
                     GKQTTPENIEAARHAFGLDKPLYVQYGRFAKGLIPWPGFFLNEDVYYSYTNFVPVKEE
                     IFARLPVTIALAVGAAVCWLLLGIPIGIISAVRRGSVWDQICDGLRADGRVGSYVLAG
                     VPALVHLLVQAPMGARLGHPDRRVGS"
BASE COUNT          526 a          901 c          988 g          524 t
ORIGIN      
        1 agcgccgcga ccgagccggt gaccatcgag ctcgatcctc tcagcagcga ggagagcgac
       61 cgcctgatcc agaacctcct cgggccgagt gccatatcca agggctaccg ggcgcgcgtt
      121 gcggacgcgg ccgaaggcaa cccgctgttc gtcgaagaga tgctcggcat gcttattgac
      181 gacgagcgcc tacgtcgcga ggagggtatg tgggtggcga cggacgagga cgtctcggtg
      241 cccacgcctc caagcatcca gagcctcctc gcggcgcgca tcgagcggct cccgcaagcc
      301 gaacgcgaga tcctgcagcg ggcgtccgtg gcggggaagg tgttctggtg gggcgccgtc
      361 gccgacctct cgccgtccga tgatcgatcg ggtgtcggtt cccgcctgca gaccctagtg
      421 cgtaggggca tgattcgtcc cgacctgagc gagttcgccg gccaggacgc cttccgcttc
      481 aagcacatcc tgatccggga cgccgcctac gaatcggtcc ccagggaact gcgcgccggt
      541 ctgcacgaac gtctcgccaa ttggatcgaa cgaaccgcag gcgaacgtgg cgacgagtac
      601 gaggagatcc tcgggtacca cctggagcaa gcgtgtcgtc agcggcttgc ctcggccggg
      661 gaacgggatc gcgagcttgc ggcgcgtgca tcgagctacc tggcctcggc gggcgatcgc
      721 gcactcgatc gcggcgatgc cagggctgcc gtcaacctcc tctcccgtgc atcgaagcta
      781 agcgcagcag aacgaccaga ggaccttgcc gtccgactct cgttgggccg ggccctgcac
      841 tattcgggcg agttccggca agccctcggg gttctagccg agctggcgaa gcaagcaatt
      901 gccgctggcg atcaacgcct cgagtggcgg gctcgaattc agcaacaaca gatcatcgtc
      961 gccaccacga ccgacgtcag cttcgaagag atccgcacca ccgccgaccg agcgatcgaa
     1021 atcttcaccg aactggacga cgatctgggt ttgttctgga gttgggagct catggcatgg
     1081 atgcatttca acgcgggacg cgcccacgaa gcgcagaagg cggcgtcgaa ggctacctct
     1141 tacgcgccct ctccagccga gctggctgac ggccttcttg acggtctgac ctatgcggtc
     1201 tttggaccta tgccagtgga ggaggctctc cgtctgtgcg gcgatgtccg ggggcacgtg
     1261 gagggggatc cagctcgcga agcaagtctg ctccagctcg aggccatact cgaggcgatg
     1321 cgcgggaaca tcgagaaggc gagagccggg attagccgag cgagatcgac gtggcaggag
     1381 cttggccatg tcaccgcgct cgcctcgttt acggagtcgg cttcagacgt cgagtggtac
     1441 gccggggatg taattggcgc ggagcgcgaa cgccggtcag gctacgaggc atggcggaag
     1501 atgcgagtcg tcgggtatca atcaacgcag gcggcattgc tggcggagct ccttgttgag
     1561 ctcggccgcg acgacgaagc tctgagtctc actagggaaa gcgagaagtt ggcagccgat
     1621 tacgacatca ctgcgcaagt cccgtggcgc ggtgcgaggg caagagttct tgctcgacga
     1681 ggggaggttg aagaggcggt gcgactcgcg cgagaggctg aggcgatggc tgagcgaacc
     1741 gattggctca aacttcaggg cgatacacag atcgacttgg cggacgtcct ccgactggcc
     1801 gggcgctccg aggaggcgac ccatgctgcg ctaaaggccg cagagctgta cgaacagaag
     1861 ggtgatatcg tagctaccgc tcgagccggg gctgtgcttg ttgggttgaa acacaagtag
     1921 gccctccctg ctggcgagtt gattcaagtc caaacgacga cgtgacacgt tcgccactca
     1981 ctcttcggct cggtgcaatc ctccgggttg tcgcgactac tcgctcccta gtggccgcgt
     2041 gcgagcagtg tgccgtcgag cccggggccc aatcagagtc ataagtcgga aaggcaggaa
     2101 aaccgaagga cgaggcagga tcccgaaaca gcaaggtccg tccgggggag cgatttcctc
     2161 cgctgggggg attaggcttg gcttggcaag cgcgatgggc aggtacctga tccggcggac
     2221 gctcttcatg atcctggtcc tgttcgtcgt gtcgttaatt acgttcatca tcttcgtcaa
     2281 gctccccgca tccgatccgg ctcggcgagc cgcgggcaag cagaccaccc ccgagaacat
     2341 agaggcggcc cggcacgcct tcgggctcga caagccgctc tacgtgcaat acgggcggtt
     2401 cgccaagggc ctcatcccgt ggccggggtt cttcctgaac gaggatgtgt actactcgta
     2461 caccaacttc gtccccgtga aggaggagat cttcgcccgg ctgccggtca cgatcgcgct
     2521 cgccgtcggc gcggccgtct gttggctgct gctggggatc ccgatcggga tcatctcggc
     2581 cgtgcggcgg ggaagcgtct gggaccagat ctgcgatggt cttcgcgctg atgggcgtgt
     2641 cggctcctac gttctggctg gggtacctgc tcttgtacat cttctggttc aagctccaat
     2701 gggcgcccgc ctcgggcatc ccgatcggcg agtcggttcc tgaagcggtc ctgcaggggc
     2761 gattcatcct gccatggctc gtgttggccc tcacgtccgc ggcgttgtac gcccggatgg
     2821 tccgcggcaa cctgatcgag tcgatgggcg aggactacat ccggacagcc cgcgcgaagg
     2881 gcctttcaga gaagcgggtg atctacacgc acggcctgcg tggggcgctc accccggtc
//
LOCUS       DASTUV010000198         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1623110, whole genome shotgun sequence.
ACCESSION   DASTUV010000198 DASTUV010000000
VERSION     DASTUV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623110"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1113)
                     /locus_tag="VFT03_04005"
     CDS             complement(238..1113)
                     /locus_tag="VFT03_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU4847337.1"
                     /translation="MERTDTVARRRQELGSETPDLIPGYFARIDKGDLLTHAEEIDLS
                     KRAKAGDKKARQRLVEKNLRLVVSVAKKYRGYGLPFEDLIQEGNIGLMKAVEKFDPER
                     GFRFSTYATWWIRQAVQRAVADKGRTIRVPVHMGDKMRKMARTYNELSTLLGRDATDE
                     EVAEELGWAVEQVRDVKSAIPDAGSLHQPLSSDEGSSELGDLIEDERASDTPGEVMRE
                     METAGLEQAIERLPERHRYVLRRRYGLDDRDTATLAELSDELDISRERVRQLQREAER
                     RLASGEFGAAFRDAA"
     gene            1696..1971
                     /locus_tag="VFT03_04010"
     CDS             1696..1971
                     /locus_tag="VFT03_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847338.1"
                     /translation="MKSGCLNLLFVLLTASLATTLIVAGLFFLLSFAAMSFESPSGAE
                     AVLKILAALIAVYFFIRVGRSVWRDLRSSSPGVGSGKPENMSPTDDE"
     gene            complement(1977..2417)
                     /locus_tag="VFT03_04015"
     CDS             complement(1977..2417)
                     /locus_tag="VFT03_04015"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU4847339.1"
                     /translation="MMSGEHPGIPAREPLGGNPTLSGPEETTGVTPGVVFVLAAGGLR
                     HLDGAGGFLGYPPGEIFWRDVLELVHEDDLSRARAVISAVIDRPGASLSTELRFRDAS
                     GSWQLMETSVQNVLEGPGDVGLLVVNVREAPPRAGEIWPPDRER"
     gene            2897..3604
                     /locus_tag="VFT03_04020"
     CDS             2897..3604
                     /locus_tag="VFT03_04020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4847340.1"
                     /translation="MSTFSVIIPALNEERHVGALLSDIASQTRVPDEVLVVDAGSEDN
                     TVAVARRFGFMRLLDGERPVARGRNLGGRSARGDVLIFLDTDTRLPERFFESFLAEFE
                     ERSLDIACPQYFPYRSTPMIESFHALFNLLTRAFEGTLPSGAGHCIAVRGKVFRESRG
                     FDPRLKFDDIELIRRLAKGRRFGIVEERIFVSDRRYKEEGTPRMILRYALMALIFALG
                     KFTWANRIEYEFGDHER"
BASE COUNT          628 a         1204 c         1131 g          701 t
ORIGIN      
        1 gcactaccgc gatgcgcgct ccggcctcgg gccgcggcgg atagattgga tcctgcttag
       61 agacccggga ggacgcctcc agctgtcgtc tcactcgatc gtgcgtgacc acgccgggga
      121 gacgtgcccg agcgaccatt atcccgtggt cgccgacctt ttatcttaag atcgacccga
      181 caaacgaaaa aagggagccc ggctttcggg ctccctcatt gttggttcag tttttcctca
      241 ggccgcgtcg cggaaggcgg ccccgaactc tccgctggcg agcctacgct cggcctcgcg
      301 ctgcagctgc ctaacccgct cgcgggagat gtccagttcg tcgctgagct cggccaacgt
      361 cgccgtgtcg cggtcgtcga ggccgtagcg gcggcgcagc acgtaccgat ggcgctcggg
      421 caggcgctcg atggcctgct ccagccccgc ggtctccatc tcgcgcataa cctctcccgg
      481 cgtgtcggag gcgcgctcgt cttcgatgag gtcgccgagc tccgaagagc cctcgtccga
      541 gctcaacggc tggtggaggc tgcccgcgtc aggtatcgcg ctcttgacgt cgcgtacctg
      601 ctctacggcc cacccgagct cctcagcgac ctcctcgtcg gtagcatcgc gtcccaacag
      661 ggtcgagagc tcgttgtaag tgcgggccat cttgcgcatc ttgtcaccca tgtgtacggg
      721 gacacggatg gtccttccct tgtcagcaac agccctctgt actgcctgtc ggatccacca
      781 cgtcgcatac gtcgagaacc gaaagcccct ctcagggtcg aacttctcta cggccttcat
      841 caaccctatg tttccctcct ggatcaaatc ctcgaaggga agcccatagc cgcggtactt
      901 cttggccaca gagactacca gcctcaggtt cttctcgacg aggcgctggc gagccttctt
      961 gtcgcctgcc ttggcgcgct tactgaggtc tatctcttct gcgtgcgtga gcagatcacc
     1021 cttgtctatc ctggcgaagt agcccgggat gaggtcaggg gtctccgatc ccagctcctg
     1081 tcgacgtctg gcgacggtat ccgttctctc catcacatct ctccttaccc tgtaaaagat
     1141 tgccgttccc cgcggtcgat ccccacgacg acatcggccc ctcggaagac gcctgcacgc
     1201 gggcgccgag agcggccgtg cccggtgtga tcgagattgg tcgaagcgaa tatggggagc
     1261 gcggagaaac ccgcccgttt atactatctc tacaaccgta tattttactc gttctgcggc
     1321 gtttgtcaag tcccagggtg ccgtgtgagg aagagacatg acaagttggc caaaaagggc
     1381 ggcccggcgc ccaacccgct cctttcccgc tttaccccgg attgggtgcc ggaccgatta
     1441 acggcgaggc cgggtaaatc gtccctggcg ccgcgcctct tggtgctgac gtcgcatcct
     1501 ccccggcgac gtcgtgcggc gtatgctagc aacgctggaa ggggagtgct ctaggttttt
     1561 tgtcccaggg cggagcgcga tacgaccatg gtgggatcgt ggagatcgcc attattcgct
     1621 ttacgaagat ccatccgtat cggggtatag ttacgcgatg gacgtggcta cgatggaggt
     1681 caggaggatg acggggtgaa gtcggggtgc ctgaacctgt tgttcgtgtt gctcacggcg
     1741 tcgttggcta cgacgctcat cgtcgcgggc ctgttcttcc ttctctcgtt cgccgcaatg
     1801 tcgttcgagt cgccctccgg ggcagaggct gttctgaaga tacttgcggc cctgatcgcg
     1861 gtctatttct tcatccgagt aggcaggagc gtctggcggg atctgagaag ctctagtccg
     1921 ggcgtggggt cggggaagcc ggagaacatg agtcccaccg atgacgaata gcgcctctat
     1981 cgttccctgt caggcggcca gatctccccg gcgcgaggcg gcgcctcgcg cacgttgacc
     2041 accaggaggc ccacgtcacc ggggccctcg aggacgttct gtaccgaggt ttccatgagc
     2101 tgccagctgc cggaggcgtc ccggaagcgc agctcggtgc tcagcgaggc tccgggccta
     2161 tctatcaccg cggagatcac ggctcgtgcc cgggacaggt cgtcctcgtg gacgagctcg
     2221 aggacgtccc tccagaagat ctcacccggc gggtagccga ggaacccacc cgctccgtcg
     2281 aggtggcgca gccctcctgc ggccaggacg aacacgacac cgggcgttac gcccgtcgtc
     2341 tcttcgggac cggagagcgt cgggttcccg ccgagcggct ctctcgccgg gatgccgggg
     2401 tgttcgccgc tcatcatcgc cgcacctcgt acacgggttc ctccccgcag aggaggaaga
     2461 acagttcgat ctccctgcgg tagcgaccga gcgcctgctt cggagaaagg tagccgccgc
     2521 gcatagagcg caggagaaaa ggaacgggcc aggggcaagt ccggccatca gatgctccat
     2581 ccatcactcc gccacctcct ggccaaccac cttaccggcg tagtagcctc cgaggcgagc
     2641 gccgaaacgt tcgcctctcc tcaggcatcg ggcgccatac acgatacctt cgccagttcg
     2701 caccaaagat gttctctgga aacgccttgc cagactcact tataagcctt cggcggcctc
     2761 tcagaaaggg ggcccgggcg gttggcgccg aaaatggccc gaagtgtgca cgatggctac
     2821 ataccccggc tcgcggagta gcagattgcc actccgggag tgagggtaag atccccccgt
     2881 ccggagcaga cacaaggtga gcactttcag cgtcatcatc cccgccctga acgaggagcg
     2941 ccacgtcggc gccctgctct ccgacatcgc ctcacagacc agggtgccag acgaggtgct
     3001 cgtcgtggac gcgggctccg aagacaatac cgtcgcggtg gcgcggaggt tcggcttcat
     3061 gaggctgctc gatggcgagc gacccgtcgc ccgcgggagg aacctgggag gccggagcgc
     3121 gcgcggggat gtcctcatct ttctcgacac cgatacccgc ctgcccgaac ggttcttcga
     3181 gagctttctc gcggagttcg aagagcgaag cctcgacatc gcctgtccgc aatacttccc
     3241 ctaccgctcc acgccgatga tagagagctt ccacgcgctc ttcaacctcc tcacaagggc
     3301 gttcgaggga accctccctt cgggcgcggg gcattgcatc gcggtcaggg gcaaggtctt
     3361 cagggagagc cgcgggttcg atccccgcct caagttcgac gacatagagc tgatccggcg
     3421 cctcgcgaag ggccggcgct tcggcatcgt ggaggagcgg atcttcgtct ccgaccggcg
     3481 ctacaaagag gagggcaccc cgcgcatgat cctgaggtac gcgctcatgg cgctcatctt
     3541 cgccctcggg aagttcacct gggccaaccg catagaatac gagttcggcg accacgagcg
     3601 ctgaacgaag gccgagcggc tccggagagg gcgtcgcgtc tcagacggct tccgttgtcc
     3661 cctc
//
LOCUS       DASTUV010000199         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_150277, whole genome shotgun sequence.
ACCESSION   DASTUV010000199 DASTUV010000000
VERSION     DASTUV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150277"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFT03_04025"
     CDS             <1..712
                     /locus_tag="VFT03_04025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4847341.1"
                     /translation="SREGARVFLAGRTREPLEAVAGDIRSAGGLAEVSVLDALDERAV
                     DEHARDVVSQAGSIDVSFNLITRGDVQGIPLVEMATEDLLRAVVNGLRTNFITARAAA
                     RRMVEQGSGVILALNSGSANGSPMMGSTGPADAAADILIRNLAAEVGPSGVRVVGIWT
                     AGLPETLSPESLAAVDSKFEDEAAFQGLIENLDQMRMTRRSPRLSEVAATAVFLASDG
                     AGAITGTFVNVTSGIFPS"
     gene            complement(897..1022)
                     /locus_tag="VFT03_04030"
     CDS             complement(897..1022)
                     /locus_tag="VFT03_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847342.1"
                     /translation="MRKYGAHRPEGLSALTAFLIIAEAALLVGILVMNRRRSLLR"
     gene            complement(1185..1739)
                     /locus_tag="VFT03_04035"
     CDS             complement(1185..1739)
                     /locus_tag="VFT03_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU4847343.1"
                     /translation="MRKVVAWELVSLDGVMESPERWSFQFHNDEMAEANASGMAAADA
                     MLFGRVTYQEFAAFWPYQNSAEQPFTDYLNNTFKYVVSTTLEEPLEWQNSTLIKDDVT
                     EVVAGLKQQPGKDIGIVGSGTLVRSLLRDGLLDELGLMIHPIVLGSGKRLFEEGGDQR
                     TLDLVGSKTFSTGVVNLTYRPAQS"
     gene            complement(1914..2255)
                     /locus_tag="VFT03_04040"
     CDS             complement(1914..2255)
                     /locus_tag="VFT03_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4847344.1"
                     /translation="MTHIINKSDLAHSETAYEFEGHHYADTRVSFIVIDAPPGSGPKL
                     HRHPYEEVFIVQEGSVTFTAGDDIIEASGGQVIVVPAGVPHKFINSGNGPLRQVDIHA
                     SERFITEWLED"
     gene            complement(2343..2741)
                     /gene="tsaA"
                     /locus_tag="VFT03_04045"
     CDS             complement(2343..2741)
                     /gene="tsaA"
                     /locus_tag="VFT03_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEU4847345.1"
                     /translation="MSIELRTIGFVSTDAEELPRHWSISDVEGTLVIDEQYSEGLRDI
                     EPGQRIVVIFNFHRSPVFSPEFLRQTSGRDGSEMGVFSICSPIRPNPIGMSVVEVTGI
                     RGSNISVRRLDMLDGTPILDIKPYIEDRRG"
BASE COUNT          494 a          934 c          867 g          554 t
ORIGIN      
        1 ctcccgcgag ggcgcacgcg tcttcctcgc cggccgtacc cgcgagccgc tcgaggcggt
       61 agccggagac atcaggagcg cgggcggctt ggccgaggtt tccgtcctcg acgccctcga
      121 cgagcgggcc gtagacgagc acgcccggga tgtagtttca caggcgggca gcatcgacgt
      181 gtccttcaac ctcatcacgc gcggggacgt gcagggcatc ccgctggtcg agatggcgac
      241 ggaagatctc ttgcgcgccg tggtcaacgg cctgcgcacc aacttcatca cggcgcgagc
      301 ggcggcgcgc cgcatggtcg agcagggctc gggggtcatc ctggcgctga acagcggctc
      361 ggctaacgga agccccatga tgggcagcac cggtccggct gatgcggccg ccgacatcct
      421 catccgcaac ctggccgccg aggtcgggcc gagtggcgtg cgggtggtgg gcatctggac
      481 ggccgggctc cccgagacgc tctcgccaga gtcgctcgcc gcggtcgaca gcaagttcga
      541 agacgaggcg gcgtttcagg gcctgatcga aaacctggac cagatgcgga tgacgcgccg
      601 ctcgccgcgg ctctccgagg ttgcggctac ggccgtgttc cttgcctcgg acggggccgg
      661 cgccataacc ggcacgttcg tcaacgtcac tagtggaata ttccccagct agccacgact
      721 ggtcgaattg ggaacacgac tccgacctca tctacgtgaa ggtcgcttga tacccgttcg
      781 aggaaccgct cgacagcatg ccggcgcgcg tgagcgtcgc gggctgctga gcaacctcct
      841 ccgggaggct ggctcaataa ggttcttcgt acgtcagagc gtccgccccc ggccggttag
      901 cggagaaggg agcggcgcct gttcatcacg aggataccga ccagcagcgc cgcctcggcg
      961 atgatcagga aggcggtaag cgccgagagc ccttccggac ggtgtgcgcc gtacttcctc
     1021 attggcgctc tcctttcgct cgaagataac cgagcagcac cgtagcaagc gcggcgcaca
     1081 acgcgcatcc cccagattgc gcaatttttc gggaagatgc caggagaacc ttcgccccgg
     1141 gaggtcgggt gggtaagcaa tgaccacctg gctctctgcc ggattcagct ctgtgcaggc
     1201 cggtaggtta ggttgacgac tcccgtgctg aaggtcttcg agcccacgag gtccagagtc
     1261 ctctgatctc ccccctcctc gaaaaggcgc ttcccgctgc ccaggacgat agggtggatc
     1321 atgagcccga gctcgtccag gagcccatcg cgaagtagcg accggaccag ggtgccgctg
     1381 cccacgatac ctatgtcctt gccgggctgc tgcttcagcc cggcgaccac ttcggtgacg
     1441 tcgtccttga tcagggtcga gttctgccac tcgagtggct cttcaagcgt cgtcgagacg
     1501 acgtatttga aggtgttgtt caggtagtcc gtgaacggct gctcggcgct gttctggtag
     1561 ggccagaacg cggcgaactc ttgataggtc acccttccga acagcatggc gtccgccgcg
     1621 gccatccccg atgcgttcgc ctccgccatc tcgtcgttgt ggaactggaa ggaccatctc
     1681 tccggcgact ccatcacgcc gtccagcgac accaactccc aggcgactac tttcctcata
     1741 gcaaactcct tcgttcgatc ctgcatctcg cccaccttgg ctggcagcat gagtatcccc
     1801 gtgatgcgtt ccttcgcacc gacgaacttc ccagacgtcg ggtgaccgtg cttcgtcgca
     1861 ggggcgagcg agagggtctc tctagagcta tccccttccc ctgggagacc ggctcagtct
     1921 tcgagccact cggtgatgaa ccgttcgctc gcgtgtatgt ctacttgccg aaggggtcca
     1981 ttgccggagt tgatgaactt gtgcgggacg ccggcaggca ccacgattac ctggcccccg
     2041 ctcgcctcga tgatgtcgtc tccggccgtg aacgtgacgc tgccctcctg tacgatgaag
     2101 acctcctcgt agggatgcct gtgcagcttc gggccgcttc ccggcggcgc gtctatcacg
     2161 atgaacgaga ccctggtatc agcgtaatgg tgtccctcga actcgtaagc agtctcgctg
     2221 tgcgccaggt cggacttgtt gatgatgtgc gtcacgccct acctcccgtg gctcacccgg
     2281 tctcccgcag cgtaactgcc gactgcccgg gtgtcgtcgt tcgtcataat gggtgctcct
     2341 ctctatccac gcctgtcttc aatgtagggc ttgatgtcga gtatgggcgt accgtccagc
     2401 atgtccagcc tcctcacgct tatgttcgag cccctgatcc cagtcacctc cactaccgac
     2461 atcccgatcg ggttgggcct gatgggggaa cagatgctga atacacccat ctcgctcccg
     2521 tcgcgccccg acgtctgccg gaggaactcg ggagaaaaga cggggcttcg atggaagttg
     2581 aagatcacga ctatgcgctg gcccggttcg atgtctcgaa gtccttcgga atactgctca
     2641 tcgatgacca gcgttccctc cacgtcggaa atgctccagt gccgggggag ttcttcggcg
     2701 tccgtactga cgaagccgat ggtcctcaac tcgatgctca tgccgaaacc ccctgtgttc
     2761 gtcccgactc tcgctcaccg ccacttcggg tacagcataa ggcaatagtc caaaaatcca
     2821 acgggagtca gaacagacag tgcgccccg
//
LOCUS       DASTUV010000200         8358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1329571, whole genome shotgun sequence.
ACCESSION   DASTUV010000200 DASTUV010000000
VERSION     DASTUV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329571"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFT03_04050"
     CDS             <1..378
                     /locus_tag="VFT03_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU4847346.1"
                     /translation="LQFVYRHFPMTTVHPHAEQAAEAAEAAGAQRRFWEMHSILFGNQ
                     QHLGTPYLHAYAEALGLDVHRFDREIADHVHADRVREDFLSGVRSGVAGTPTFYINGV
                     RHDDSYDLPVLLTALQRAASLGA"
     gene            500..2191
                     /locus_tag="VFT03_04055"
     CDS             500..2191
                     /locus_tag="VFT03_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847347.1"
                     /translation="MPLDETPDRNGEYPRLSQAQIEALARRGERRRTQPGEVLFREGD
                     KGYDFVVVLDGKVAVIAGYGGEEALIAVHGPRRFLGELSLLTGQAAFFTAVVGEPGEV
                     LAVPADRLRELATQNPALGDLILRAYLLRREMLIGLGAGFRIVGSRYSPDTRRLREFA
                     SRNRLPHRWSDLEADEAAEALLRGLGVAPEETPVVIWRGNEVLRNPSNAELARRLGLR
                     ELRAVEDVCDLLVVGAGPAGLAATVYGASEGLVTVTLDAVATGGQAGTSSRIENYPGF
                     PSGLSGAELAERAEIQAEKFGARINVPAETTALDQRDGYHVLELDDGTTVSARTVLIA
                     TGARYRKLSVPRIEEFEGTSVFYAATLMEAQLCRNALVAVVGGGNSAAQAALFLADHT
                     QNVWLLIRHSGLGRDMSRYLADRIERNPDIEVLLHTEVRELIGDEALEALIVEDNQTG
                     ERRRLEARDLFVFIGAEPYTRWLEGQLALDDRGFILTGHDAARSARQGAPQQLGYEPY
                     LLETSRPGVFAAGDVRSGSIKRVASAVGEGAMVVRLVHARLGDRGHLEQNPGQNT"
     gene            complement(2336..3445)
                     /locus_tag="VFT03_04060"
     CDS             complement(2336..3445)
                     /locus_tag="VFT03_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4847348.1"
                     /translation="MKQLDGGASRVAPARPRSRPTRIRISRRTRNAILVLGLAAVVWL
                     MWAAPTVPVVLLGGFALAMALSFPVRWFSRLMPRGLAILFTFAILVGIGALALLFLVP
                     ILIDQLASLISAAPDVARQARKIPRGVLEPLTRLGLWHGTPDQLMSRLGQDLVNLAQN
                     LAQQVLGGLVRFISITFGIALTLFGVIFVAVYLLANVRKLKAAYLRAAPKRYRSDARE
                     LWDAFAYSLTRYLSGLGLDMFAQGALSAVGLFLLGVPYALLLGTWVALTAVIPYLGAW
                     LGAVPGVVVALTISPTKALLTALLFLAIQQLDGNVLQPTIQGRVLNLPSILIFLAVIA
                     GGEIAGLLGVIFAVPTLAVLKVLFDFFRTRLETEV"
     gene            complement(3507..3788)
                     /locus_tag="VFT03_04065"
     CDS             complement(3507..3788)
                     /locus_tag="VFT03_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847349.1"
                     /translation="MAGKEQSFWDRIFSIHHHSDREEKVLEYIIHRLGDGADLDEVAR
                     EEYVRRHASPDEVQEILDNPRLVESIRKKMEEDFEELGEVLARARGADA"
     gene            complement(3798..4244)
                     /locus_tag="VFT03_04070"
     CDS             complement(3798..4244)
                     /locus_tag="VFT03_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 family protein"
                     /protein_id="HEU4847350.1"
                     /translation="MPIGVSNFERFFRAAASLDVDKDDLRRYEDFVNRKVRDLLLISR
                     AAARANGRDIIEPWDLPITKGLQETIHDFREMDEAAEVRPMLEQIVAIPPQELAYSEE
                     TEGRLPEIAGGLSLALARAFKIIEPRLKNPQTEHWERAYRVFDLLL"
     gene            complement(4275..4967)
                     /locus_tag="VFT03_04075"
     CDS             complement(4275..4967)
                     /locus_tag="VFT03_04075"
                     /inference="COORDINATES: protein motif:HMM:NF025933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4847351.1"
                     /translation="MSEHLTRIVASCEPRGHEKEVGRLIEAARESGADALALLGDLGE
                     GGDVREYARVLRTVGRGANVPVFYVPGPGDAPFGTFLREAHNTEVVFPSMHGVHGTFA
                     FAPGFHVVFAGMGGEVADDPHHEREERERLSYPGWEVEYWLKVLKETKDYQKVFLFHT
                     PPAHKGLQERGSEVLAELVKTYAPRLVLTGGEEFRQERLGTSLVVAPGRLSGGEYALV
                     DIHEREAERKKL"
     gene            complement(5009..6388)
                     /locus_tag="VFT03_04080"
     CDS             complement(5009..6388)
                     /locus_tag="VFT03_04080"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4847352.1"
                     /translation="MNRLAITGLVAGGAVAAAGLLRLALKPRPRYEPSERPPYGEFPN
                     RVLVVGGGFAGYTAAETLCELTKDRDDVGVLVLSRENYFTFWPMVPGIVGSEVDIDNV
                     AQPLRRSLIDAGASFRRAELQSIDFERKVAVADGGKEFPYDHLILALGSQPNFFGVPG
                     VREHSLALGGLADALRIRNRVIERFEQATLDPVEPPDPKLTFVVIGGGSTGVETAAEI
                     HALIHEALAPDFPNIDVDEVEIFVIEGGPEILAELDPALRRAAGAELAARGIEVITGV
                     RATEVTADRVKLHDGREIETENVVWTAGIRPNAKLEDLDLPRTERDGVVVDERLRVVD
                     RPDVWAIGDSAAIPQVLGGLAPPTAQAAVQEGRVAARNVLAAIDGRQEDMVRFEYRPL
                     GVLVELGSRFAVNDVMGVRFSGLVAALFWRAVYLSKLESPQNKVRVAADWILGLFFRP
                     AATEIRDLA"
     gene            complement(6429..6968)
                     /locus_tag="VFT03_04085"
     CDS             complement(6429..6968)
                     /locus_tag="VFT03_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEU4847353.1"
                     /translation="MNDGGSRVEPKREARVPYYAEPEHTDVHDRGSRLMELLQDVVVV
                     LLNLTLLGMAFVYLYRVWVEIFAFGNLQEGLSNIIFVVITIELYRLSVHYLRYHRVDL
                     SILVEVGVSAIIQKVILIGVDKLSMQQLVGISLILAALGAILWVHMRARYFGSPLSFG
                     RRTRGLRRAENDEGSGATS"
     gene            complement(6965..>8358)
                     /locus_tag="VFT03_04090"
     CDS             complement(6965..>8358)
                     /locus_tag="VFT03_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4847354.1"
                     /translation="DDTIDILRGLRDRYEAHHRVKITEEAIIAAAELSDRYIPDRFLP
                     DKAIDLMDQAAARVRLRSRTKPEDTRALEDEVRRLNREKDEAVANEDFERARNLRDGI
                     ARLEADLEEVKGGRRRPVPEVTAEDIAEVVSRATGIPVSQLTEEERSRLLRLEQQLHE
                     RVVGQDEAVEAVAEAIRRARAGLADPNRPIGSFLFLGPTGVGKTELARTLAEALFGEE
                     AAMIRIDMSEFQERHTVSRLVGAPPGYVGYEEAGQLTEAVRRRPYSVLLLDEIEKAHP
                     DVFNILLQILDDGRLTDAQGRTVDFKQTVIIMTSNMGSERIQAHSSMDESFEDLKEDM
                     MQVVRTHLRPEFVNRIDEIIVFRELTKEQIAEIARLMLDRTKRRLRAQDIEVEFTEAA
                     VELIAEEGFDPEFGARPLRRAIQRRVDNEISRMVLGSVLNPGDRVVVGAEEGRLTFEV
                     LEGAAAAVAGETE"
BASE COUNT         1338 a         2817 c         2753 g         1450 t
ORIGIN      
        1 ctccagttcg tgtaccgcca cttccccatg acgaccgtgc atccccacgc cgagcaggca
       61 gccgaggctg cggaggcggc cggtgctcaa cggagatttt gggagatgca ctcaatattg
      121 ttcgggaacc agcaacacct cggcaccccc tacctccacg cctacgccga agctctcggc
      181 ctggacgtcc accgattcga cagggagatc gcggatcatg tccacgccga ccgcgtccgc
      241 gaggacttcc tgagcggggt gcgcagcggg gtggcaggca cgccgacgtt ctacatcaac
      301 ggggtgcgcc acgatgactc ttacgacctt ccggtcctgt tgaccgccct tcaacgggcg
      361 gcgagcctgg gagcgtgagg aagcacgctc gccgacaccc gcgacgatat acaaggaggt
      421 ttgccgtgct cgcaacctac agagacccgc ggcgtcgata tcttcccggc tagtggcggc
      481 tgtggacgct agagcgccgg tgccgctcga tgagacaccc gaccgcaacg gagagtatcc
      541 gcggctaagc caggcgcaga tcgaggctct cgccagacgt ggcgagcggc ggcgcacgca
      601 gccaggcgag gtgctgtttc gggaaggcga caaaggatac gacttcgtcg tggtcctgga
      661 tgggaaggta gctgtcatag cgggctacgg gggcgaggag gccctgatcg ccgtacacgg
      721 ccctcgccgc tttctcgggg aactcagcct gctcaccggc caggctgcct tcttcacggc
      781 ggttgtgggc gagccgggtg aggtgctcgc ggtgcccgcg gatcgccttc gcgagctggc
      841 cacccagaac cccgcgctcg gggatctcat cctgcgcgcc tacctcctcc gccgcgagat
      901 gctcatcgga ctcggcgccg gattcaggat cgtaggctcg cgctactcgc cggacacgcg
      961 gaggcttcgc gagttcgcct cccgcaaccg gctgccgcac cgctggagcg acctggaggc
     1021 ggacgaggcg gcggaggccc tgctgcgcgg gcttggcgta gcgcccgagg agacgccggt
     1081 cgtgatctgg cgcggcaacg aggtgctgcg caacccgagc aacgcggagc tggcgcgcag
     1141 gctcggcctg cgggagctgc gcgccgtgga ggacgtctgc gatctcctgg tcgtcggggc
     1201 tggtcccgcg gggctcgccg ccaccgtcta cggagcctcg gagggcctcg tgacagtcac
     1261 actcgacgcg gtcgcgacgg gcggccaggc cggcacctct tcgcggatcg agaactatcc
     1321 cggcttccct tcagggctct ctggcgcgga gctcgcggag cgggcggaga tccaggcgga
     1381 gaaattcggc gcgcgcatca acgtgccggc ggagacgacc gctctcgacc agcgcgacgg
     1441 gtaccacgtc ctcgagctcg acgacggaac gaccgtctca gcccggacgg ttctgatcgc
     1501 gacgggcgcc cgctaccgca agctatccgt gcctcgcatc gaagagttcg agggaacgtc
     1561 cgtcttctac gccgcgacct tgatggaagc gcagctgtgc cgcaacgcgc tggtagcggt
     1621 cgtgggaggt ggcaactcgg ccgcgcaggc cgctttgttc ctggccgatc acacgcagaa
     1681 cgtctggctg ctgatccggc acagcggcct ggggagggat atgtctcgct acctggctga
     1741 ccgaatcgag cgaaacccgg acatcgaggt gctactccac acggaggtgc gagagctgat
     1801 cggggatgaa gctctcgagg cgctcatcgt ggaggacaac cagacagggg agcgccgcag
     1861 gctcgaggca cgggatctct tcgtcttcat cggcgccgag ccgtatacgc gctggctcga
     1921 aggccagctc gccctcgatg atcgtggttt catacttacc gggcacgacg ccgcccgctc
     1981 ggcgaggcag ggcgctccac agcaactcgg gtacgagccg tacctgctcg agaccagcag
     2041 acccggcgtg ttcgcggcgg gagacgtaag aagtggatcg atcaaacgcg tggcttcggc
     2101 cgtgggcgaa ggtgccatgg tcgtccgcct cgtgcacgcg cggctcgggg accgcgggca
     2161 tctcgagcaa aaccctggac agaatacctg aggagcacct ggggacctcg ctagtctcgc
     2221 tcgatacgcc cgggggcctc tcttacagtc gtttctccgc tctctatcct tgtctgcgct
     2281 ttcctcacga gagctctgcc ctgcgggtat ggagtctgct ctggaaggtg catgatcaga
     2341 cctccgtctc cagacgggtc cggaagaagt cgaagaggac tttcaggacc gcgagcgtgg
     2401 gcacggcgaa gatcaccccg agaagccccg ctatctcccc gcccgctatg acggccagga
     2461 agatgaggat cgagggcaaa ttgaggaccc gcccctgtat ggtcggctgc aagacgttgc
     2521 cgtcgagctg ctgtatcgcg aggaagagga gggcggtaag cagcgccttt gttggcgaga
     2581 tggtgagagc gaccaccacc ccgggtacgg cgccgagcca cgctcccagg taggggatga
     2641 cggccgtgag cgcgacccag gtcccgagca gcaacgcgta gggaacgccg agcaggaaga
     2701 gccctaccgc agagagtgcg ccctgcgcga acatgtcgag gcccagcccg ctcaggtatc
     2761 gcgtgagcga gtaggcgaag gcgtcccaca gctcgcgggc gtcggacctg tagcgtttcg
     2821 gggcagctct gaggtaggcc gccttgagct tgcgcacgtt ggctagcagg tagacggcga
     2881 cgaagatcac cccgaagaga gtgagcgcga tgccgaaggt gatcgagatg aagcgcacca
     2941 ggccgccgag cacctgctgc gcgaggttct gggcgaggtt cacgaggtcc tggcccagcc
     3001 tgctcatgag ctggtccggc gttccgtgcc agagcccgag ccgggtgagg ggctcgagca
     3061 cgccgcgcgg gatcttcctg gcctgacgcg cgacatcggg ggcggcgctt atgagggagg
     3121 cgagctgatc tatgaggatg ggcacgagga agaggagggc gagcgctccg atgccgacca
     3181 ggatcgcgaa ggtgaacagg atagccagcc ctcgaggcat caggcgggag aaccaccgca
     3241 caggaaagga gagcgccatc gccagcgcga aaccgccgag cagcacgacc ggaaccgtcg
     3301 gggccgccca catgagccac accacggcgg cgagcccgag cacaaggatc gcgttccggg
     3361 tcctcctgga gatcctgatc cgggtgggcc gggaccgggg ccgtgccgga gctacccggc
     3421 tggctccccc gtccagctgc ttcatcggtc tccaccctcg agccgcttcg aacggggaaa
     3481 ttctgatcct cgcgccgcgg ccaccgtcac gcgtccgcgc cccgcgccct ggccaagacc
     3541 tcgccgagtt cctcgaagtc ttcttccatc ttcttgcgga tcgactcgac gagcctgggg
     3601 ttgtccagta tctcctgaac ctcgtccggg gaggcgtggc ggcgaacgta ctcttcccgg
     3661 gccacctcgt cgaggtcggc cccgtccccc aggcggtgga tgatgtactc gaggaccttc
     3721 tcctcccggt ccgagtggtg gtggatgctg aagatcctgt cccagaagga ctgttctttc
     3781 ccggccacgg tccctcctta caggagcagg tcgaagaccc tgtaggcccg ctcccagtgc
     3841 tcggtctgcg ggttcttgag cctgggctca atgatcttga aggcgcgagc gagggcgagg
     3901 ctgaggcccc cggcgatctc gggcagcctg ccctcggtct cctcgctgta ggccagctcc
     3961 tgcggaggta tggcgacgat ctgctccagc atcgggcgca cctcggccgc ctcgtccatc
     4021 tccctgaagt cgtggatggt ctcctggagc cctttggtga tgggtaggtc ccagggctct
     4081 atgatgtcgc gtccgttggc cctggccgcg gcgcggctga tcaagagcag gtcgcggacc
     4141 ttgcggttga caaagtcctc gtagcgtcgc aggtcgtcct tgtcaacgtc caggctcgcg
     4201 gccgccctga agaatcgttc gaagttgctt accccgattg gcaccttgct gctccctccc
     4261 gtaagtaggc gtcgctagag ttttttccgt tccgcctcgc gctcgtggat atcgacgagg
     4321 gcgtactctc ccccgctcaa cctccccggt gcgaccacca gcgaggtccc gagacgctcc
     4381 tggcggaatt cctccccgcc ggtcagcacc aggcgaggag cgtacgtctt gacgagctcg
     4441 gctaggacct cgcttcccct ttcctgcaat cccttgtggg cgggaggggt gtgaaagagg
     4501 aacaccttct ggtagtcctt cgtctctttg agtaccttga gccagtactc gacctcccag
     4561 ccggggtagc tgagccgctc ccgctcctcg cgctcgtggt gcggatcgtc cgccacctcg
     4621 ccgcccatcc cggcgaagac gacgtggaac ccgggcgcga aggcgaaggt cccgtggacg
     4681 ccgtgcatcg aggggaagac cacctccgtg ttgtgcgcct ccctcaggaa ggtgccgaac
     4741 ggggcgtcgc ccgggcccgg gacgtagaaa acgggaacgt tggcccctcg ccccaccgtc
     4801 ctgagcaccc tcgcgtactc gcgcacgtcc ccgccttcgc cgaggtcacc cagcagggcc
     4861 agcgcgtcgg ccccgctctc ccgggccgcc tcgatcagcc gccccacctc cttctcgtgc
     4921 ccgcgcggct cgcaactcgc gacaatcctc gtaaggtgct cgctcaaggt ttcttacctc
     4981 ctgttcacag gtgtcttcgt gttctcacct aggcaaggtc ccggatctcc gtcgccgcgg
     5041 ggcggaagaa gaggcccagg atccagtccg ccgcgaccct caccttgttc tggggactct
     5101 ccagcttgga gaggtagacg gcccgccaga agagggcggc cacgagcccg gagaagcgca
     5161 cacccatcac gtcgttcacg gcgaaccggc tgccgagctc gacgagcacg cccagcggcc
     5221 ggtactcgaa cctgaccata tcctcctgcc tgccgtcgat ggcggcgagg acgttcctgg
     5281 ccgcgacgcg tccctcctgg accgcggcct gggcggtcgg cggggccagg ccacccagta
     5341 cctgggggat ggccgcggag tcgcctatcg cccagacgtc gggacggtcc accacacgca
     5401 gccgttcgtc gaccacgacc ccgtcgcgct cggtgcgggg taggtcgagg tcctcgagtt
     5461 tggcgttggg ccggatgccg gcggtccaca caacgttctc ggtctctatc tcccgcccgt
     5521 cgtggagctt cacgcgatcg gccgtcacct cggtcgcgcg gacgccggtg atcacctcga
     5581 tgcccctggc cgcgagctcc gcgccggccg cacggcgcag cgcggggtcg agctcggcga
     5641 ggatctccgg cccgccctcg atgacgaaga tctcgacctc gtctacgtcg atgttcggga
     5701 agtcgggggc cagcgcttcg tggatgagag cgtggatctc ggccgctgtc tcgacccccg
     5761 tggacccgcc gcctatgacg acgaaggtga gcttcgggtc gggtggctct acggggtcga
     5821 gggtggcctg ctcgaagcgc tctatgacgc ggttgcggat cctcaaggcg tccgcgagcc
     5881 cgcccagcgc gaggctgtgc tccctgacgc cggggacgcc gaagaagttc ggctggctgc
     5941 ccagcgccag gattaggtgg tcgtagggaa actctttgcc gccgtcggcg acagcgacct
     6001 tgcgttcgaa atcgatgctc tgaagctcgg cccgccggaa gctcgccccc gcatctatga
     6061 gggacctgcg caggggctgg gcgacgttgt cgatgtccac ctcgctgccg acgatccctg
     6121 gcaccatcgg ccagaaggtg aagtagttct ctctggagag aaccaacacc ccgacgtcgt
     6181 ctctgtcctt cgtgagctcg cataaagtct ccgccgccgt gtacccggcg aacccgccac
     6241 ccacgacgag caccctgttg gggaactcgc cgtagggtgg cctctccgag ggctcgtacc
     6301 tcggcctcgg cttcagggcc agcctcaaga ggccggccgc ggcgaccgcg ccgcctgcca
     6361 ccaggccggt tatcgcgagc cggttcataa gatccttccg taacgtctgc catcgagcgt
     6421 accggtcact agctcgtcgc ccccgatcct tcgtcgttct ctgcccgccg gagcccgcgg
     6481 gtcctgcgcc cgaaggagag ggggctgccg aagtatctcg cgcgcatgtg cacccacaat
     6541 atggcgccta gggcggcgag gatcagggag atccccacga gttgctgcat ggagagtttg
     6601 tctaccccga tcaggatcac cttctggatg attgccgaga cgccgacctc gacgaggatg
     6661 ctcaggtcca ccctgtggta tctcaggtag tggaccgaga gccggtagag ctcgatcgtg
     6721 atcaccacga agataatgtt cgagaggccc tcctgaagat ttccgaaggc gaagatctct
     6781 acccacaccc tgtagaggta cacgaaggcc ataccgagca gcgtcaggtt gagcaggacg
     6841 accacgacgt cctgcaggag ctccatgaga cggctccccc tgtcgtggac gtcggtgtgc
     6901 tcgggctccg cgtagtacgg aacccgagct tccctttttg gctcgaccct cgagcccccg
     6961 tcgttcactc ggtctcaccg gcgacggcgg ctgccgcccc ctcgagaacc tcgaaggtga
     7021 gcctcccttc ctcagcgccg acaaccactc tgtcgcccgg gttcaggacg ctgccgagca
     7081 ccatgcggga gatctcgttg tccaccctgc gctggatggc gcggcgcaaa ggccgtgccc
     7141 cgaactcggg gtcgaagccc tcctcggcga tgagctcgac cgcagcctcg gtgaactcca
     7201 cctcgatgtc ctgggcgcgc aggcggcgct tggtgcggtc cagcatcagc cgcgctatct
     7261 cggctatctg ctccttcgtc agctccctga agacgatgat ctcgtctatg cggttcacga
     7321 actcgggcct gaggtgggtc cgcacgacct gcatcatgtc ttccttgagg tcctcgaagg
     7381 actcgtccat ggaggagtgg gcctggatcc tctcggaccc catgttggag gtcatgatga
     7441 tgacggtctg cttgaagtcc accgtgcggc cctgggcatc cgtcaggcgc ccgtcgtcga
     7501 ggatctggag caggatgttg aagacgtcgg gatgcgcctt ctcgatctcg tcgaggagta
     7561 gcaccgagta cgggcgccgc cgcacggcct ctgtgagctg gcctgcctcc tcgtagccga
     7621 cgtagccggg aggggcgccg acgagccggc tcacggtgtg ccgctcctgg aactcgctca
     7681 tgtcgatgcg gatcatcgcc gcctcttccc cgaagagcgc ctcggctaga gtgcgcgcga
     7741 gctcggtctt cccgacgccc gtaggaccga ggaacaggaa gctgcctatc ggacggttgg
     7801 ggtccgctag gccggccctg gcgcgcctga tggcttcggc gaccgcctcg acggcctcgt
     7861 cctgtccgac aacccgctcg tgcagctgct gctcgagccg gaggaggcgt gagcgctcct
     7921 cttcggtaag ctgcgagacc gggatgcccg tggccctgct gacgacctcg gctatgtcct
     7981 ctgccgtgac ctcaggtacg ggcctcctgc gtccgccctt cacctcttcg aggtctgcct
     8041 ccagccgggc gatcccgtcc ctgaggttcc gagccctctc gaagtcctcg ttggccaccg
     8101 cctcatcttt ctcgcggttg aggcgcctca cctcgtcctc cagagccctc gtgtcctcgg
     8161 gcttggtcct cgagcgcagc ctgacccgcg cggccgcctg gtccatgagg tctatggcct
     8221 tatcagggag gaagcggtcg gggatgtagc ggtcggagag ctcggccgcc gcgattatcg
     8281 cttcttctgt tatcttcacc ctgtggtgag cctcgtagcg gtcccgcaag ccacgcagga
     8341 tgtctatggt gtcgtcga
//
LOCUS       DASTUV010000201         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_762045, whole genome shotgun sequence.
ACCESSION   DASTUV010000201 DASTUV010000000
VERSION     DASTUV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762045"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFT03_04095"
     CDS             <1..423
                     /locus_tag="VFT03_04095"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847355.1"
                     /translation="ESFLDELLPQGRELQVVEAIREDMRLLSDAPRLVRDLLGPVDPG
                     AFAGELPESSDEVFARAAGALETRRLDSLEDAQDLVGELRAWAKERGMRSRDLLHPLR
                     LALTGRNRGPEMARLFMVLGAQESQERIERAREARLRT"
     gene            420..1838
                     /gene="cysS"
                     /locus_tag="VFT03_04100"
     CDS             420..1838
                     /gene="cysS"
                     /locus_tag="VFT03_04100"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU4847356.1"
                     /translation="MSVHVRDTLSGGLVEIGREGEIGLYVCGPTVYNHIHIGNARAPL
                     FWDVAVRYLKSRGYDVTYVQNITDVEDKIINKANEEGISWEEVVRRYTDSFHERLEML
                     GVGLPNEEPRATEYIPEMISLIQELIEEGHAYAPSEANGDVYYDVESFPSYGKLSHQR
                     PEEMRETEKGQTGFKKSPLDFALWKASKPGEPSWESPWGPGRPGWHIECSAMVEKHLP
                     SGADVHGGGTDLLFPHHENELAQSCGAHPDHTFVRAWVHHGMVNFVGDKMAKSIGNVV
                     DVAEAVERHGSNAIRMWLLQSHYSQPIDYSDEILEEKRRSYERLIRLYRQIAGSTDSS
                     ELSDRLAAGLLERFDAAMRDDLNTPEAIAAVFEVAGRAGQEISTRPEAAREFSSLSEA
                     LLEVLTIFGFDLAEELAADVDGVRVRYSEEPGEGVLRLVAARELARRERDWPVADRLR
                     GELANEGWAVEDTPEGPILSRR"
     gene            1842..>2340
                     /locus_tag="VFT03_04105"
     CDS             1842..>2340
                     /locus_tag="VFT03_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU4847357.1"
                     /translation="MPETIYGVRPVTEALRGGRREVSEVLDSTGNPEVVQAAKAAGVP
                     VKKVSQARVGELARGGVHQGVAARVGPYPYVDLKDLLAASDPLVVVLDGVTDPHNLGA
                     VLRVAEGAGASGVVIPKDRAVGVTAAAVKASAGASEHVPVARVTNIRRAIDRMKEAGL
                     WVYAAE"
BASE COUNT          419 a          731 c          834 g          356 t
ORIGIN      
        1 gagtcgttcc tcgacgagct cctgccgcag ggccgcgagc tccaggtggt ggaagccatc
       61 agggaggaca tgcggctcct ctccgacgcg ccgcgcctgg tgcgcgacct cctaggtccg
      121 gttgaccctg gcgccttcgc cggggagctg ccggagtcga gcgacgaggt cttcgcgcgt
      181 gcggccggag ctctcgagac tcgccggctg gatagtctgg aggacgcgca ggacctcgtc
      241 ggagagctgc gcgcgtgggc taaggagcgt gggatgagat caagggatct gctccacccc
      301 ctgcgcctgg cgcttacggg gcggaacagg gggccggaga tggcgcgcct tttcatggtg
      361 ctcggggcgc aggagtcgca ggagaggata gagagggcga gagaggctag actacgcaca
      421 tgagcgtgca cgtgcgggac actttgagcg gcgggctggt cgagatcggt cgggaagggg
      481 agatcgggct ctacgtctgc gggcctacgg tctacaacca catccacatt ggtaatgcgc
      541 gggcgccgct gttctgggac gtggccgtga gatacctcaa gagccggggc tacgacgtta
      601 cctacgtcca gaacataacg gatgtcgagg acaagatcat taacaaggca aacgaggagg
      661 ggatttcctg ggaagaagta gtgcgccgct acaccgactc cttccacgag cgtctcgaga
      721 tgctcggcgt cggcctcccc aacgaggagc cgcgcgctac agagtacata ccggagatga
      781 tctctctcat tcaggagctc atagaggagg gccacgccta cgctcccagc gaagctaacg
      841 gggatgtcta ctacgacgta gagagctttc cgagctacgg caagctttcc caccagcgtc
      901 ccgaggagat gcgcgagaca gagaagggcc agacgggctt caagaaaagc ccgctggact
      961 tcgccctgtg gaaagcgtcg aagccgggcg agccctcgtg ggagagcccg tgggggccgg
     1021 ggcgcccggg ctggcacatc gagtgctcgg cgatggtcga gaagcacctg ccctccggcg
     1081 cggacgtgca cggtggcggc accgacctcc tcttccccca ccacgagaac gagctcgccc
     1141 agagctgcgg ggcgcatccg gaccacacct tcgtccgcgc ctgggtccac cacggcatgg
     1201 tcaacttcgt cggggacaag atggcaaagt ccatcggcaa cgtcgtggac gtcgcggagg
     1261 ccgtggagcg ccacggcagt aacgctatcc ggatgtggct cctgcagagc cactactcgc
     1321 agcccataga ctactccgac gagatactgg aggagaagcg ccgctcttac gagcgcctca
     1381 tacgcctgta ccgtcagatc gccggctcga cagactcatc cgaactatcc gacaggctcg
     1441 ccgccgggct tttggagagg ttcgacgcgg cgatgcgcga cgacctcaac acccctgagg
     1501 ccatcgccgc ggtcttcgaa gtcgccggca gggcggggca ggagatctcc acccgccccg
     1561 aggccgctcg cgagttctcc tcgctatccg aggctctcct ggaagttctg acgatcttcg
     1621 ggttcgacct cgctgaggag ctcgccgccg acgtcgacgg ggtgcgtgtc cggtactccg
     1681 aagagccggg ggagggcgtt ctgcgcctgg tcgccgccag ggagcttgcg cggcgcgaga
     1741 gggattggcc ggttgccgac aggttgcgcg gcgagctggc caacgagggc tgggcggtcg
     1801 aagatacccc cgaaggtccc atcctcagcc gccggtagcc cgtgccggag accatctacg
     1861 gcgttcgccc tgtgaccgag gcgctgcgcg gcgggcgccg ggaggtctcc gaggtcctgg
     1921 actcaacggg caaccccgag gtcgtgcagg ctgcaaaagc ggccggcgtg ccggtgaaga
     1981 aagtctccca ggctcgggtc ggagagctcg cccgcggcgg ggtacaccag ggggtggcgg
     2041 cgcgggttgg accgtacccc tacgtcgatc tgaaagacct gctggcggcc tcggatcccc
     2101 tcgtagtggt cctcgacggc gtgaccgacc cgcacaacct cggggcggtg ttgagggtcg
     2161 cagagggggc gggggcgagc ggcgtcgtca tcccgaagga cagggccgtg ggcgtcacgg
     2221 ccgccgcagt caaggcgagc gcgggcgcta gcgagcacgt tcccgttgca agggtcacaa
     2281 acatccgaag ggccatagat cgcatgaagg aggccggcct ctgggtctac gccgccgagg
//
LOCUS       DASTUV010000202         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_763772, whole genome shotgun sequence.
ACCESSION   DASTUV010000202 DASTUV010000000
VERSION     DASTUV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763772"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2312)
                     /gene="ppdK"
                     /locus_tag="VFT03_04110"
     CDS             complement(<1..2312)
                     /gene="ppdK"
                     /locus_tag="VFT03_04110"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEU4847358.1"
                     /translation="MIGRGKLVYGFYEGSGEMRDLLGGKGAGLAEMTRLGLPVPPGFT
                     ITTEACRAYTEAGRLSAGLMESVTEYLEVLEEVTGKRLGDSDNPLLVSVRSGAAVSMP
                     GMMDTVLNLGLNDAAVAGLARRTGDERFAYDSYRRFIQMFGDIVLKIPHERFEEALEI
                     LKEERGVEEDPQLEAADLKELVNVYKEIVEAEADRPFPEDPAEQLELAIRAVFDSWDN
                     ERAIAYRREFGIPDCLGTAVTVQAMVFGNMGEDSATGVAFTRDPSTGEQGIFGEFLLN
                     AQGEDVVAGIRTPRPLEEMIEAMPHAFSELLETMQRLEHEYRDMQDVEFTVERDALYM
                     LQTRSGKRTAAAAIKIARDMAEEGFIRKDEALLRIVPGQLEQLLHPRMDPVAEVEVLC
                     TGLGASPGAATGKIVLTAQEAAERGGAGEAVILVRKETDPDDVEGMMRAKGVLTALGG
                     MTSHAAVVARGMGKPAVTGCSALKIDSHAGAFSVNGRVFEAGDVITIEGTSGRVIAGE
                     VPLVEPEITEDFETILRWADRVRALGVRANADTPEDARRAREFGAEGIGLCRTEHMFM
                     EEGRLRIVREMILSEDERALDAALEKLEPLQKEDFEKIFKAMDGLPVTVRLLDPPLHE
                     FLPDSKDLATQVAEAEARGENAGELVKQLRVAERLEEVNPMLGLRGVRLGVLRPEVYR
                     MQARAIAAAARTVAEEGYVPVVEIMVPLVGFPSELAMIKAYVEEEVGETPVKIGTMIE
                     LPRACAVAGEIAEDADFFSFGTNDLTQMVCG"
     gene            2573..>3250
                     /locus_tag="VFT03_04115"
     CDS             2573..>3250
                     /locus_tag="VFT03_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847359.1"
                     /translation="MGVSVLDPSPVSGPLLAVAAAPKGLHRAALHLLDLPKPAFLDSS
                     LTDGHGGRYSFFTADPFLVLRSRGRRVELIGPAGRAVTEEDPWVLLQLLLRRYPVDRV
                     AGLPLFLGGAVGYFGYDLGRTLERLPATAADEGLPELDVGFYDWVLAADHLSGESWIV
                     ATGLPTGQEADARARVAGIQERLNDTPSSSSNAASLMAPRLHSNFRRVDYLQAIERAR
                     DYVAAGDI"
BASE COUNT          549 a         1121 c          938 g          642 t
ORIGIN      
        1 ccgcatacca tctgggtgag atcgttggtg ccgaatgaaa agaagtccgc gtcttcggct
       61 atctcgccgg cgacagcaca tgccctgggc agctcgatca tggtcccaat cttcacgggc
      121 gtctccccca cctcctcctc aacgtaggcc tttatcatcg ccagctcgga ggggaaaccg
      181 accagcggca ccatgatctc caccaccggg acgtatcctt cctcggctac cgttctagcg
      241 gcggcagcga tggcccgcgc ctgcatccgg tagacctccg gccgcagtac tccaaggcgg
      301 accccccgca gcccaagcat cgggttgacc tcttcgagtc gttcggcaac ccgcagttgt
      361 ttcacgagct cgccggcatt ctctccgcgg gcctcggcct ccgctacctg cgtcgccagg
      421 tccttggagt cggggaggaa ctcgtgcagc ggcgggtcca ggaggcgtac ggtcaccggc
      481 agcccgtcca tggccttgaa tatcttctcg aagtcttcct tttggagggg ttctagcttc
      541 tccagtgccg cgtccaaggc tctctcatcc tcggagagga tcatctcccg cactatccgg
      601 aggcgtccct cctccatgaa catgtgctcg gtgcggcaca gtccgatccc ttcggcgccg
      661 aactcgcggg cgcgccgggc gtcctccgga gtgtcggcgt tcgcccggac cccgagtgcg
      721 cgtactctgt ctgcccagcg gagaattgtc tcgaagtcct cggtgatctc gggctccaca
      781 agcgggactt cacccgcgat caccctgccg ctcgtaccct cgatggtgat gacgtccccg
      841 gcctcgaaaa ccctgccgtt cacgctgaac gcgccggcgt gggagtctat cttcagagcc
      901 gaacacccgg tgaccgccgg tttgcccata ccccgcgcta ccaccgccgc gtggctggtc
      961 atgccgccca gggccgtaag cacgccttta gcgcgcatca tgccctccac gtcatcgggg
     1021 tcggtctctt tacgtaccag gatcaccgct tccccggccc cgccgcgctc ggcggcctcc
     1081 tgggccgtca ggacaatctt gcccgtggcc gctccggggg acgctccaag gcccgtgcac
     1141 aggacctcga cttcggctac ggggtccatc cgcgggtgga gcagctgctc cagctggccg
     1201 ggaactatgc gcagtagagc ctcatccttg cggatgaacc cctcctcggc catgtcccgg
     1261 gcgatcttga tcgccgccgc agcagtcctc ttgccgctcc tcgtttggag catgtagagt
     1321 gcatcccgct ccacggtgaa ctccacatcc tgcatgtcac ggtattcgtg ctcaagtctc
     1381 tgcatcgtct cgagaagttc ggagaaggcg tggggcatcg cctctatcat ctcctccagg
     1441 ggccggggcg tacggatacc cgccacgacg tcttcgccct gggcgttgag caaaaactcc
     1501 ccgaagatcc cctgctcccc ggttgacggg tcgcgcgtga atgctacccc agtggccgag
     1561 tcctcgccca tgttgccgaa gaccatagcc tgcaccgtga cggcggtgcc caggcagtcc
     1621 ggaatgccga attccctgcg gtaggctatg gccctttcgt tgtcccagga gtcgaagact
     1681 gcccgaatcg cgagttcgag ctgctctgcg ggatcctctg gaaacggacg gtcggcctcc
     1741 gcctcgacga tctccttgta aacgttcacc aactccttga gatccgcggc ttccaactgc
     1801 gggtcctcct cgaccccgcg ctcttctttg aggatctcga gggcctcctc gaacctttcg
     1861 tgcgggattt tgagcacgat gtccccgaac atctggatga agcgccggta ggagtcgtac
     1921 gcgaagcgct cgtccccggt tcggcgcgcg agcccggcca cggcggcatc gttgaggccg
     1981 aggttcaata ccgtgtccat catgcccggc atggagaccg ccgctccgct acgtaccgag
     2041 acgagcagag gattatcaga atcccctaaa cgcttgccgg tgacctcctc aagaacctct
     2101 aggtactcgg tcacggactc catgagaccc gcagagagcc tcccagcttc cgtgtacgct
     2161 cggcaagcct ccgtggtgat cgtaaacccc ggaggaaccg gcaggccgag gcgggtcatc
     2221 tcagccagtc ctgctccctt gccaccgagt agatccctca tctcccccga cccctcgtag
     2281 aagccataga ccaatttgcc cctacctatc attggatgcg cccctttacg cgctcttcgc
     2341 aagcagtccg tccggttacc gagcgtccgc gaagccccac cacatcacct caattttgtt
     2401 gcgctatttg ctaatgttgc tcataatgga acagatacca taaagttctg cactgagtca
     2461 agggggatca gttttgaaac ctctttcttt aggagacttg aagacgcgaa gctacccccg
     2521 acaggaggtc gttaagtttc gcaacgacgc ctcggaagag gtggtgagaa ggttgggtgt
     2581 aagtgtactg gatccgtcgc cggtgagcgg tcctctcctc gccgtcgctg cggcgcccaa
     2641 aggcctgcac cgggcagcac tccaccttct cgacctgccg aagccggcgt tcctggacag
     2701 cagcctgacc gacggccacg gcgggcgtta ttcattcttc acggccgatc ccttcctggt
     2761 tttacgaagc cggggtcgcc gcgtcgagct tatcggtccg gcgggacggg cggtcacgga
     2821 ggaggatccc tgggtccttc tccaactcct gctgcgccgc taccccgtgg atcgggtggc
     2881 aggactgcca ctgtttctgg gcggagccgt tggctatttc ggctacgatc taggccgcac
     2941 gctggaacgg ctgcccgcca ccgccgccga tgaaggacta ccggagttgg acgtcggttt
     3001 ctacgattgg gtactggccg ccgaccacct gtccggtgag agctggatcg tcgccacggg
     3061 gcttccgaca ggccaggagg ccgacgcacg cgctcgggtc gccgggatcc aagagcgact
     3121 gaacgatact ccatcctcgt cgagcaacgc cgcaagccta atggccccgc gactgcactc
     3181 gaactttcgg cgcgtcgact acctccaggc catcgagaga gccagagact acgttgccgc
     3241 gggcgacatc
//
LOCUS       DASTUV010000203         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_579214, whole genome shotgun sequence.
ACCESSION   DASTUV010000203 DASTUV010000000
VERSION     DASTUV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579214"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VFT03_04120"
     CDS             <1..1551
                     /locus_tag="VFT03_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4847360.1"
                     /translation="YLVRRLRPGRRLCVVLTWRGEQIPNRLRGLVTEGRAATVLRLGR
                     LDRAAVEELVGSVVGGTEGLGQRLYEETEGLPLFVGEYLAAIAKGSLAAGDESWSLPG
                     GVRDLLHGRLGTVGETGVQLLNAAAVIGRSFDFDIVREASGRSEEEAVAALEELTDKG
                     LVEEVQGVGERSPAYDFGHEKLRALVYEETSLARRRLLHRRVAEALVRRARRRREIGP
                     LAGQIAHHYRSAGRDAEAAEYFELAGQHSRALYANREALGHFSAALALGHPDAAALHQ
                     AIGDLHTLLGEYPAALASYEAAAALGDGSGLASVERKLGSVYQRLGEWDLAESHFEAA
                     LAQGDAEPAWVRARLHADLGLVAQRQGRDELALEQASRALELAEAAGDGRALTQAHNV
                     LGVLASGSGNLDAARQHLERSLELAEAAGDPAARVAALNNLALAYRNGGEPERALERA
                     EAALALCVSIGDRHREAALHNNLADLLHEAGRSQEAMRHLTQAVTIFAEVGEDDKLQP
                     EIWKLVEW"
     gene            complement(1564..1989)
                     /locus_tag="VFT03_04125"
     CDS             complement(1564..1989)
                     /locus_tag="VFT03_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847361.1"
                     /translation="MSLEYERGETQMKLSTVMQWGGPVAIVSGVFTMVSDLLGLTIYV
                     PGLGEAANTGYQAVGAGVILFALMLLIVGMAGLYAGQPKPGDPRVIEIGYPVPEGMEA
                     GQRTNSPELRFTRRAAAGPRRGHRTSPAHQASFEGYRRA"
     gene            complement(2147..3610)
                     /locus_tag="VFT03_04130"
     CDS             complement(2147..3610)
                     /locus_tag="VFT03_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4847362.1"
                     /translation="MGTRPGPAPHHLMAGSGAYDAVVVGAGPNGLAAAVELARNGCSV
                     AVLEAEETPGGGTRSAEITLPGFVHDLGSAIHPLGYASPFFSELPLEEYGLEWVHPPA
                     PLAHPFDDGTAAVMERSVQETAGPLGSDAPAYRRLMESVAEDVPRLASYFVGSPRLIR
                     HPIALGASALRALRPARSLAESAFEGEKARGLFAGNAAHSFLPLEKSPSALFGLLLGT
                     LGHAFGWPFPKGGSQRIADALTSYLLDLGGEVYTGFRVDSVEEVPRTRAVLLDVTPRQ
                     LLDIAGEHFTERYRHALKDYRYGPGVFKVDFALNGPAPWKAEECLRAGTVHLGGTLDE
                     ISAGEAAVWRGEHPERPFVLLAQQSLFDRTRAPEGKHTVWAYCHVPNGSTFDMTGRIE
                     AQIERFAPGFGERVLAKTASGPADLERQNANLVGGDINGGVPELRQLFTRPVLKPNPY
                     STPAQGIFVCSSSTPPGGGVHGMCGYFAARVALRYLS"
     gene            complement(3574..4341)
                     /locus_tag="VFT03_04135"
     CDS             complement(3574..4341)
                     /locus_tag="VFT03_04135"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HEU4847363.1"
                     /translation="MTEATPPPEHRDEKQPRDAAFWAQRVERLEVSDVPVGAANVNVQ
                     GRREVGALQGFGQLWQKTYRVRLNGVEVSPEEVVRIWKERFPEYQPPNSRFYPSMAGV
                     APGEVLFISASVGGMPVHTGARVIYADDESFTVMTPEGHPESGWNTFSAYSDEEGVAV
                     AQVQSLARANDPIYELGFRVVGSTEQERIWTHVLKSLAAHFGVNEPVTLEKACVDPKV
                     QWSQAKNVWYNAGARSMIYTMAGPARWVRGLVRRRTT"
BASE COUNT          758 a         1487 c         1582 g          731 t
ORIGIN      
        1 tacctcgtgc ggcgcctcag acccgggcga aggttgtgcg tcgtgctcac ctggcgcggc
       61 gagcagatcc ccaaccgcct gcgggggctc gtgacggagg gccgcgcggc gacggtgctc
      121 cgtttggggc gcctggacag agccgccgta gaggagctgg tcgggtcggt cgtcgggggc
      181 acggaggggc tgggacagcg cctgtacgag gagacggagg ggttgccgct cttcgttgga
      241 gagtacctcg cggcgatcgc gaagggctct ctggccgcgg gtgacgaatc ctggtcgctg
      301 ccgggcggag tgcgcgacct cctgcacggg cgcctgggaa cggtcggcga gacgggggta
      361 cagttgctca acgccgccgc ggttatcggc aggtccttcg acttcgacat cgtgcgcgag
      421 gcgagcggtc gcagcgagga agaggcggtc gcggcactcg aggagctcac ggacaaaggt
      481 ctggtcgagg aggtgcaggg ggtcggagag cggtcgccag cctacgactt cggccacgag
      541 aagctgcgcg ccctcgtcta cgaggagacc agtctggcga ggagacggct attgcaccga
      601 agggtggccg aagcgctagt gcggcgcgcg cggcggcggc gcgagattgg accgctggcg
      661 ggccagatcg cgcaccacta ccggtcggct ggcagggacg cggaggcggc agagtacttc
      721 gagctggcgg gccagcactc cagggcgttg tacgctaatc gcgaggcgct cggtcacttc
      781 agtgcggcgc tcgcgctggg gcacccggat gccgccgcac tccatcaggc tatcggcgac
      841 ttgcataccc tgctcggcga atacccagcg gccctcgcga gctacgaggc agcggccgcg
      901 ctcggtgacg ggagtggcct cgccagcgtc gagcgcaagc ttggcagcgt ctaccagcgt
      961 ctgggggagt gggatctcgc agagagccac ttcgaggctg cgctggcgca gggagacgcc
     1021 gagccggcct gggttcgcgc caggctccac gccgacctcg gtctggtggc tcagcgccag
     1081 ggacgggacg aactggcgct ggagcaggca agccgggcgc tcgagctcgc cgaagccgcc
     1141 ggagatgggc gggcgctgac tcaggcccac aacgtcctcg gcgtgctagc gagcggcagc
     1201 ggcaacctcg atgctgctcg ccagcacctg gaacgcagcc tcgagcttgc tgaggccgcc
     1261 ggggaccctg cagctcgagt cgccgcactg aacaacctgg cactggccta ccggaacggc
     1321 ggtgagccag aacgagccct ggaacgcgcc gaggcagccc tggctctatg cgtgtctata
     1381 ggggatcgcc atcgcgaggc tgcgcttcac aacaacctgg ccgacctcct ccacgaggcc
     1441 ggacggtcgc aagaggccat gcgccacctc acgcaggccg tgaccatctt cgccgaggtc
     1501 ggcgaagatg acaagctgca acccgagatc tggaagctgg tggagtggtg agggtatccg
     1561 ccgtcaggct ctcctatatc cctcgaagga agcctggtga gccggagagg tcctgtgccc
     1621 gcggcgaggt cccgcagcgg cgcgccgggt gaaccgtagt tctggagagt tggtcctttg
     1681 cccggcttcc atgccttcgg gtacagggta gcctatctcg atcaccctcg ggtcgccggg
     1741 cttcggctgg cccgcgtaca gtccggccat gcccactatc aggagcatca gggcgaagag
     1801 gatcacgccc gccccgacgg cctggtagcc cgtattggcc gcttccccga ggccggggac
     1861 gtagatcgtc aggccgagga ggtcggagac cattgtgaac acaccactaa ctatggcgac
     1921 gggaccgccc cactgcataa ccgtcgagag tttcatctgc gtctctcctc tctcgtattc
     1981 caggctcatg cacatactct aacgggcgcc cgttacagga ccgttacgga aagcgttacg
     2041 aaaagacgct cacgaaaatt tctttcggtt tactaccttc tggtggatct ccgccacaac
     2101 gagacgtgca ccggctcccg caccaccgca ggctcctacg ggctcgtcaa ctcaggtagc
     2161 gcagggctac gcgggcggcg aagtagccgc acatgccgtg cacgccccct ccgggcggcg
     2221 tcgaggagga gcagacgaag atcccctgcg ccggcgtcga gtaggggttg ggcttcaaga
     2281 ccgggcgggt gaagagctgc cggagctcgg gaacgccgcc gttgatgtcg cctccgacca
     2341 ggttggcgtt ctgccgctcg aggtcggcgg gtcccgaggc ggtcttcgcg aggacacgct
     2401 ccccgaagcc gggggcgaag cgctcgatct gggcctctat ccgccctgtc atgtcgaagg
     2461 tcgagccgtt ggggacgtgg cagtaggccc agacggtatg cttgccctca ggcgcccgcg
     2521 tccggtcgaa gagactctgc tgcgcgagca acacgaacgg cctctcggga tgctcgccgc
     2581 gccacacggc ggcttccccg gcggagatct catccagagt tccgccgagg tggacggtcc
     2641 cggcccgcag gcactcctcc gccttccagg gggccgggcc gttgagcgcg aagtccacct
     2701 tgaagacacc cggtccgtag cggtaatcct tgagggcgtg ccggtagcgc tcggtgaagt
     2761 gctcccccgc gatgtcgagc agctggcggg gcgtgacgtc gaggaggacc gcgcgcgtgc
     2821 gcggaacctc ctcgacggag tccaccctga agccggtgta gacctcgccg cctaagtcga
     2881 gcaggtacga ggtcagcgcg tcggctatcc tctgcgagcc gcccttcggg aacggccagc
     2941 cgaaggcgtg tcccagggtg cccaggagga gcccgaagag ggcactcggg gacttctcca
     3001 gcggcaggaa cgagtgggcc gcgtttccgg cgaagaggcc gcgggccttc tccccctcga
     3061 acgcgctctc ggccaggctg cgggccgggc ggagcgcacg cagcgccgag gcacccaggg
     3121 cgatggggtg acggatcagc cgtggcgagc cgacaaagta ggatgctagc cttgggacgt
     3181 cttctgccac gctctccatg agcctccggt aagctggcgc gtcggagcct agagggccgg
     3241 ccgtctcctg cacggagcgc tccatgacag cggccgtgcc atcgtcgaac gggtgggcga
     3301 gcggagcagg ggggtggacc cactcgaggc cgtactcctc gagcgggagc tcgctgaaga
     3361 aaggcgaagc gtaccccagg gggtggatgg ccgagccgag gtcgtgaacg aagccgggaa
     3421 gtgtgatctc cgccgagcgc gtgcccccgc cgggagtctc ctcggcctcg agcacggcca
     3481 cggagcaccc gtttctagcc agttccacgg cggcggcgag accgttcggc cccgcgccca
     3541 cgaccaccgc gtcgtacgcg ccgcttccgg ccatcaggtg gtgcggcgcc ggaccaggcc
     3601 gcgtacccaa cgggccgggc cggccatcgt gtagatcatg gagcgggcac cggcgttata
     3661 ccagacgttc ttcgcttgcg accactgaac cttcgggtcc acgcaggcct tctccagggt
     3721 taccggctcg ttcacgccaa agtgcgcagc aagcgacttg aggacgtgcg tccagatccg
     3781 ctcctgctcc gtggagccca ccaccctaaa gcctagctcg tagatcgggt cgttggcgcg
     3841 agccagcgac tggacctggg caacagcaac accctcctcg tcgctgtaag ccgagaaggt
     3901 gttccagcca gactcaggat gcccttctgg ggtcataacc gtgaaggact catcgtcggc
     3961 gtagatcaca cgcgctcccg tgtgtacggg catccctccg acggaagcac ttatgaacaa
     4021 aacctctcca ggggcgactc cggccatgga agggtagaag cggctgtttg ggggctgata
     4081 ctctgggaag cgctccttcc agatccgcac cacctcctcg ggactcacct cgaccccatt
     4141 caggcgcacc ctgtaggtct tctgccagag ctgaccgaag ccctgcaaag cccctacctc
     4201 acgacggccc tgcacgttga cgttggcggc tcccacaggc acgtcggaga cctccaagcg
     4261 ctctaccctc tgtgcccaga aggctgcgtc gcggggttgc ttctcatctc tgtgctccgg
     4321 tggtggggtc gcttcggtca tgaggttgct cctttcggtc ggaaagccgg tcagactgtt
     4381 ggttctagca gtctctaacg cactctcgcg caactttgac ttcatttcga accgttcact
     4441 cgagcgaacc gcctctccgc cgttcgtggc tggctggctt gcggtgcgca ggccgcgaga
     4501 acgcatagag cgcggagagg atgagggcgt tgtgtctgat gttcgtgtac cggcgcca
//
LOCUS       DASTUV010000204         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1186861, whole genome shotgun sequence.
ACCESSION   DASTUV010000204 DASTUV010000000
VERSION     DASTUV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186861"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFT03_04140"
     CDS             complement(<1..668)
                     /locus_tag="VFT03_04140"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU4847364.1"
                     /translation="MGGPMRVGIIADDLTGAADTGVQLARAGYRTAVAFRDAPIPPAD
                     DLDAVALDTDSRAMPAGFAAKRVMEAGLTVREAYIVYKKLDSTLRGPIAAELAAALGA
                     SGRDRAVVAPAFPAAGRTTVEGVQLVRGVPVDETEAKDDPRTPVHEGHVPTLLAAAFP
                     SVVSLSAEDLADPATVRRALEEARCVVADAARDEDLEALVRAVPDPSEVLWAGSAGLA
                     VALGR"
     gene            complement(894..1646)
                     /locus_tag="VFT03_04145"
     CDS             complement(894..1646)
                     /locus_tag="VFT03_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4847365.1"
                     /translation="MSENRSRLAVVTGASSGIGAATARALAGGGFEVALGARRVHRIE
                     KLAAEIGGQARFLDVTDSGSIEEFASWTESLGGASVLVNNAGGAKGLEPVAEMVEENW
                     RWMFETNVLSVGLMTRALLPQIRRSANGHVVIIGSAAGVEAYPGGSGYNAAKFAAHAI
                     KENLRLELLGEDVRVTEILPGAAETEFGLVRFGDDEERAEAPYKGITPLTADDVAECV
                     RWAVTLPPHVNVDRIDVKPVRQAGVARFVRDA"
     gene            complement(1804..>2340)
                     /locus_tag="VFT03_04150"
     CDS             complement(1804..>2340)
                     /locus_tag="VFT03_04150"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEU4847366.1"
                     /translation="ERDLRWGVYVTFKAPTDYAARCFSEYGLVTDDSGFYTALYRPYH
                     LIGLELGISVAHAALRGEATGSATGFRGDVAATAKRDLAAGEELDGEGGYAVYGRLMP
                     AEDSLALGALPIGLAHGIELRNAVATGEFVRWSDVAIDEGQEPVRLRREMEQWSRTRG
                     MGEPSGTASMSSTGRLVE"
BASE COUNT          374 a          838 c          757 g          371 t
ORIGIN      
        1 cggccgaggg cgacggcgag gcctgccgac ccggcccaca gcacctccga ggggtccggc
       61 acagcccgca cgagggcctc caagtcctcg tcgcgcgccg cgtcggcgac gacgcaccgg
      121 gcttcctcta gcgcccggcg gaccgtagcg gggtcggcga ggtcttcggc gctcagggag
      181 acgaccgagg ggaacgccgc ggcgagcagg gtcgggacgt gtccctcatg gaccggggtg
      241 cgcgggtcgt ctttcgcctc ggtctcgtct acgggcacgc cgcgcacgag ctgcacacct
      301 tccactgtgg tgcgccccgc cgcggggaaa gctggcgcga cgaccgcgcg gtcgcggccg
      361 gaggcgccta aagccgcggc gagctccgcg gcgatgggac cccgcagtgt ggagtcgagc
      421 ttcttgtaga cgatgtaggc ctcgcgcacg gtgagtccgg cctccatcac ccttttcgcg
      481 gcgaagccgg ccggcatggc cctggagtcc gtgtcgagcg ccacagcgtc gaggtcatcc
      541 gccggtggta tcggggcgtc gcggaaagcg accgcggtcc ggtagccggc gcgggcgagc
      601 tgcaccccgg tatccgcggc gccggtaagg tcatctgcga ttatccccac gcgcatgggc
      661 ccacccaata agctcgctcc tctggctaaa aatagcatct tttcaggccc agcaggtaga
      721 gaccaggctc tgatttgacc cgatgcaagc atcgtaataa catacgatac aaggttgggg
      781 aaaacctggg ggtatcgaag gaggacgaaa ccaatggccg aagccacgga acgacggggc
      841 gaactcgccg tcgagtcgtc cggggtagcc ggcgtcttat cgctcgggga ccttcacgcg
      901 tcacggacaa accgcgcgac gcctgcttga cggacgggct tgacgtcaat gcggtccacg
      961 ttgacgtgcg ggggcagggt gacggcccac ctcacgcact cggcgacgtc gtccgcggtg
     1021 agtggcgtga tgcccttgta cggcgcctcg gccctctcct cgtcgtcccc aaagcgcacc
     1081 agtccaaact cggtttcggc agcccccggg aggatctccg tgacccgcac gtcctccccg
     1141 aggagctcca agcgcaggtt ctccttgatg gcatgggcgg cgaacttggc cgcgttgtag
     1201 cccgagccgc cggggtacgc ctcgaccccc gcggccgagc cgatgatcac gacgtgcccg
     1261 ttggcggagc gccggatctg gggcaggagc gccctcgtca tcagcccgac gctcagcacg
     1321 ttcgtctcga acatccagcg ccagttctcc tcgaccatct ccgccaccgg ctccagtccc
     1381 ttcgcacccc ccgcgttgtt gacgagcacc gaggcgccgc ccaaggactc cgtccacgag
     1441 gcgaattcct caatggagcc cgaatcagtg acgtcaagga acctggcctg ccccccgatc
     1501 tcggcggcca gcttctcgat ccggtggacc cgccgggcgc ccagggccac ctcgaagcct
     1561 cccccggcca aggcccgcgc ggtggcggct ccgatcccgg acgaggcccc ggtcacgacg
     1621 gcgaggcgag atctgttctc ggacacgtat cctcctttgt gtgcgcggct taggcgttga
     1681 agaccgatct ctgaccgttt accaggatgt tcgagcccga ccctctggat caaactcttt
     1741 tcatccaacc gacgattagg ggcctctgca gcgggcagcg ccacggcgta gcggccgaag
     1801 aagctactcg acgagccttc ctgtgctcga catgctagcg gtgcccgagg gttcacccat
     1861 gcccctggtt cgggaccatt gctccatctc gcggcgaagc cttaccggct cttgaccctc
     1921 gtcgatggcg acatcggacc acctcacgaa ctccccggtc gccaccgcgt tgcgcagctc
     1981 gataccgtgg gcaagcccta tgggtagcgc gcctagcgcc agggagtctt cagccggcat
     2041 gagacgcccg tacacagcgt agcctccctc cccgtccagc tcttctccgg cggccaggtc
     2101 cctcttggcc gtcgccgcta catcgccccg gaacccggtg gcggaccccg tcgcctcccc
     2161 tcgaagggcc gcgtgggcga cgctgatgcc gagctcgagc ccgatcaggt ggtacggccg
     2221 gtagagcgcc gtgtagaacc cggagtcgtc ggtcacgagc ccgtactccg agaagcagcg
     2281 cgccgcgtag tccgtcggcg ccttgaacgt cacgtacacc ccccagcgca ggtcgcgttc
//
LOCUS       DASTUV010000205         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_42567, whole genome shotgun sequence.
ACCESSION   DASTUV010000205 DASTUV010000000
VERSION     DASTUV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42567"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1528)
                     /locus_tag="VFT03_04155"
     CDS             complement(203..1528)
                     /locus_tag="VFT03_04155"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU4847367.1"
                     /translation="MLTQTDKALDLAERLGGERDGEFAPSGIPVSEIAREYGTPFYLY
                     HGETVVERVRRVRGALGTEVSYSVKANPSLGVCQLIAREKAAGAEVASSGELAVARAA
                     GFEPEDIVFAGPGKTDDELKRVVQEGIFADNVESLGEIERLAQIAAGMGRKIGVGLRI
                     NPAAQLMGSQMRMGGTVGQFGMDQAELEEAVEKTLSHPDLILRGVHVYTATQVFEVEP
                     LLEHCRNIFEIALEAADYAGHPLEMIDFGGGFGVPYFEKMSEFDLERFGEGFRELLST
                     YRSDPRLEGCRFLFELGRYLVADAGVYVTRAVDVKRTRGKTFVVTDGGMNHHLTATGN
                     MGQVFRKSYPLLNLTRMGGVPEEGIAVAGPCCTPLDMFGSNIPLADPEVGDLIGVFYS
                     GAYGFSASNLGFLSHPAPAEVLLWQGEAHQLRAGGKPEDVLDGQEPLES"
     gene            complement(1509..>3062)
                     /locus_tag="VFT03_04160"
     CDS             complement(1509..>3062)
                     /locus_tag="VFT03_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU4847368.1"
                     /translation="DLLHNAVERDPGGVAVVDGSTEYTYEDLERASNSLGAALIESGV
                     EKGDRVGVYMEKSWEAIVAMLAASRVGAAYVNINPLFKPPQVAYVAQDCDVRVMIGDS
                     PKLDDLEPGTVRGTAFYRGERPGEESAGHLTDLAEVFRNADAPRAESQVSEIDLLTIL
                     YTSGSTGMPKGVATSQRNVVVGAQIVSSYLENTAEDRILSALPLNFDAGMSQFTTSLR
                     VGATLVLQRSRLPGDLSRALRRHEITGVTGVPPLWSLLLRSAKAIEAEPLESLRYIAN
                     TGGRIPQANLDQLRRLLEPSGTRIYLMYGLTEAFRSTYLPPEEVDRGSTCIGKAIPNT
                     EILVIDRHGRECAPDEPGELVHRGPTVAMGYWGNEEATLKAYRPNPLAPPELLDVERV
                     VYSGDTVRRDEEGFLYFIGREDAMIKSQGYRLSPEEVENLLIGSGLVHEACAFGVEDP
                     EVGQLVMAVVSLRDGEHKGAVEDIREHVIKNGPPYMVPKEIFVLDELPKTGSGKIDRK
                     GISNAYADG"
BASE COUNT          506 a         1024 c          984 g          548 t
ORIGIN      
        1 tcacgtacta cacgaggacg aacaacggca aggtcgacta caaggaccag tgggagaccg
       61 agtacggcgc cctaatagac gacaagggga agaagatccc cactcccaag gtgcccgtgg
      121 cggagatcga cggcacctac ctcgggccta gcttctagaa agcttctggt tatttgctga
      181 agcagaaccc gatacctgaa gcctaactct ccagcggctc ctggccgtcg agaacgtcct
      241 cgggcttgcc tcctgcgcgc agctggtgcg cctcgccctg ccagaggagc acttcggctg
      301 gggcggggtg gctcaggaaa ccgaggttgc tcgcggagaa accgtaggcg ccgctgtaga
      361 agacgccgat gaggtccccg acctcggggt cggcgagtgg gatgttggag ccgaacatat
      421 cgagcggcgt gcagcagggg cccgccaccg cgatgccctc ctcgggcacc ccgcccatgc
      481 gcgtaaggtt cagcaggggg taggacttgc ggaagacctg gcccatgttg cccgtcgcgg
      541 tcaggtggtg gttcatgccg ccgtcggtga cgacgaaggt cttgccccgc gtccgtttca
      601 cgtccaccgc ccgcgtgacg tagacccccg cgtcggcaac gagatagcgg ccaagctcga
      661 agaggaagcg gcacccttcg aggcgcgggt ccgagcggta ggttgagagc agctcccgga
      721 agccctcgcc gaatcgctcc aggtcgaact cggacatctt ctcgaagtag ggcaccccga
      781 acccgccgcc gaagtcgatc atctcgagag ggtggccggc gtagtcggct gcctcgaggg
      841 ctatctcgaa gatgttgcgg cagtgctcga ggagcggctc gacctcgaag acctgggtcg
      901 cggtgtagac gtggacgccg cgcaggatca gatccggatg agacagggtc ttctccacag
      961 cctcctcgag ctcggcctgg tccatgccga actgacctac cgtcccaccc atgcgcatct
     1021 gcgagcccat gagctgggcc gcagggttga tgcggagtcc aaccccgatc ttccgtccca
     1081 tacctgcggc tatctgcgcg agacgctcga tctcgcccag cgactcgacg ttgtcggcga
     1141 agatgccttc ctggacgacc cgctttagct cgtcgtcggt cttgcccggg ccggcgaaga
     1201 cgatgtcttc gggttcgaag ccagcggccc gcgccacggc gagctcgccg gaggaggcaa
     1261 cctctgcgcc agccgccttc tcgcgggcta tgagctggca caccccgagg ctcgggttcg
     1321 ccttgaccga gtaggagacc tcggtgccca gagcgcctct gacgcggcgg acccgctcta
     1381 cgaccgtctc cccgtggtag aggtagaagg gagtgccgta ctctcgcgct atctcggaga
     1441 cgggtatgcc agagggggcg aactccccgt cgcgctcccc gccgagacgc tccgccaggt
     1501 cgagtgcttt atccgtctgc gtaagcattg ctaattccct tgcggtcgat cttgcctgaa
     1561 ccggtcttgg gcagctcgtc gaggacgaag atctccttcg gcaccatgta cggcggcccg
     1621 ttctttatga cgtgctcgcg gatatcttcg acggcgccct tgtgctcgcc gtccctgagc
     1681 gacacgacag ccatgacgag ctggcccacc tcgggatcct ctaccccgaa ggcgcacgcc
     1741 tcgtgcacga gaccgctgcc aatgaggagg ttctccacct cctcggggct caggcggtac
     1801 ccctggctct tgatcatagc gtcctcgcgg ccgatgaagt agagaaagcc ctcctcgtcc
     1861 cgccggaccg tgtctcctga atagacgacc cgctccacgt cgaggagctc gggcggggcg
     1921 agggggttcg ggcggtacgc cttcagggta gcctcctcgt tgccccagta gcccatcgcc
     1981 accgtcgggc cgcggtggac cagctctccc ggctcgtcgg gcgcgcactc ccggccatgc
     2041 ctgtctatca ccaggatctc ggtgttcggg atggccttgc ctatacaggt ggagccgcga
     2101 tccacctcct ccggcggcag gtacgtggag cggaacgcct ctgtgagccc gtacatcagg
     2161 tagatcctcg tccccgacgg ctccagaagg cgcctcagct ggtccaggtt ggcctgaggt
     2221 atgcgcccgc cggtgttggc tatgtagcgc aagctctcga gaggctctgc ttcgatggcc
     2281 tttgcgctcc tgagcagcag cgaccacagc ggcggcacgc cggtgacgcc cgtgatctcg
     2341 tgccgcctga gcgcccgcga aaggtcgcca ggcagccggg agcgctgcaa caccagtgtc
     2401 gctcctaccc gcaaagaggt ggtgaactgg ctcatcccgg cgtcgaagtt gagcgggagc
     2461 gcagagagga tgcggtcttc tgcggtgttc tccagatacg acgagacgat ctgggctccg
     2521 accacgacgt tacgctggct cgtcgccacg cccttgggca tccccgtcga gcccgacgta
     2581 tagaggatcg tcaacaggtc tatctccgag acctgactct ccgcccgtgg cgcgtctgcg
     2641 ttccggaaca cctctgcgag gtccgtgaga tgccctgcgc tctcctcgcc ggggcgctcg
     2701 cctctgtaga aggccgtccc cctgacagtc cccggctcca ggtcgtcgag cttgggggag
     2761 tcaccgatca tgacgcgcac gtcgcagtct tgcgccacgt aggcgacctg aggcggcttg
     2821 aagagcgggt ttatgtttac gtaggcggca cctacccgcg aggcggcgag catcgccact
     2881 atggcttccc aggacttctc catgtagacg ccgacgcggt cgcctttctc gacccccgac
     2941 tcgatgagcg cggcgcccag ggagttgctc gcgcgctcga ggtcctcgta ggtgtattcg
     3001 gtgctgccat ctacgacagc tacccctccg gggtcgcgct ccacggcgtt gtggagcagg
     3061 tc
//
LOCUS       DASTUV010000206         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_877554, whole genome shotgun sequence.
ACCESSION   DASTUV010000206 DASTUV010000000
VERSION     DASTUV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877554"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..530
                     /gene="def"
                     /locus_tag="VFT03_04165"
     CDS             36..530
                     /gene="def"
                     /locus_tag="VFT03_04165"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4847369.1"
                     /translation="MALDVRTFGDPVLKTRAAPVESFDDALAHLADEMLATMREHEGV
                     GLAANQIGRLKRILVAATEDEEYVIVNPVIEALAETTEKELEGCLSIPGIQVEVERPT
                     GLTLSGQDASGSPLRLETSGILARIFQHEIDHLDGVLILDRTDRESRKVAMREMRQRL
                     IAQS"
     gene            544..1464
                     /gene="fmt"
                     /locus_tag="VFT03_04170"
     CDS             544..1464
                     /gene="fmt"
                     /locus_tag="VFT03_04170"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU4847370.1"
                     /translation="MKIAFAGTPDFAATVLRGLISSGHEVGLVVSQPDARKGRGRRAQ
                     PTPVAQLARSLDLPLRQPARMSEVAGEISSNDALVVAAYGQILRPDTLYAANEGAWNV
                     HASLLPKYRGAAPIERSIMAGEPESGVTIIRMDEGLDTGPMALQRPLPIPPDMTGGEL
                     SELLACLGAEAVVEVLNQIESGTVNLIEQDSLHATYAPKLLDEDLEIRWDRSVEDVAN
                     LVRALSPHVGARAFNSGVEGPVKIWRAEVFDGEVPTLKAGAIRTENGRILVGCGVGVL
                     EVLELQMPGGKRLAARDFLRGNTLEGAFDR"
     gene            1647..2261
                     /locus_tag="VFT03_04175"
     CDS             1647..2261
                     /locus_tag="VFT03_04175"
                     /inference="COORDINATES: protein motif:HMM:NF036822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU4847371.1"
                     /translation="MQRIDLDEEIRVAIVGAGGYGRVALDVLLASGLGDRVLGFYDDA
                     HAALSGEVRGVPILGDIGMLKSMLSVEPVHVVVAITDNGTRLRVANSLRALGARFITT
                     LHPDAYLSGAALVGDGCVLAAGAVVHPDAAVGSHCFVGPGAVVDRDAEVGAGTWLSAG
                     CVVGPGARVGARVVLGQNSSVGRKAVIVGDAKVGALRHVDREDA"
     gene            2287..>2857
                     /gene="pilM"
                     /locus_tag="VFT03_04180"
     CDS             2287..>2857
                     /gene="pilM"
                     /locus_tag="VFT03_04180"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEU4847372.1"
                     /translation="MSGFDPGGVGVMDRLGGLGRAGARTIGLDVDRGALKAVQISGSA
                     GELTLRHVGYHRLPPGAVVDGEVADVEVLGAEIREFWSSHSFKGKSVNLGVANGRVVV
                     RLLDFPRMEAEDLKSAITFEAQDHIPMPLDEAVLDYVVLGPQSEGSDLDRILVVAAHK
                     DMIGGYTAALRAGGLKPAGIDVKALALTRS"
BASE COUNT          502 a          890 c          980 g          485 t
ORIGIN      
        1 tgcgtgtgga cgtagacccc gaggaggtgt agatcatggc gctagatgta agaacgttcg
       61 gagacccggt gctcaagaca cgtgccgcgc cggtcgagag cttcgacgac gcgctggcgc
      121 acctcgccga tgagatgctg gccaccatgc gcgagcacga gggtgtgggg ctcgccgcca
      181 accagatagg tcgcctcaaa cgcatcctcg tcgcggctac cgaggacgag gagtacgtca
      241 tcgtcaaccc tgtcatcgaa gcactcgccg agactaccga gaaggaactc gaaggatgcc
      301 tctcgatacc gggcatccag gtcgaggtcg aaaggccgac gggcctgacg ctctcgggtc
      361 aggacgcctc gggttccccc ctgaggctgg agacctcggg cattctcgcg cggatcttcc
      421 aacacgagat cgaccacctc gacggcgtct tgatcctgga ccgcaccgac cgcgagtccc
      481 gcaaggtcgc tatgcgcgag atgcgccagc gccttatcgc ccagagctaa tccccgccat
      541 cccttgaaga tagccttcgc aggcactccc gacttcgccg ccacagtcct gcgcggcctc
      601 atctcctccg gccacgaggt aggcctcgtc gtctcccaac ccgacgccag gaaaggtagg
      661 ggccgccgtg cgcaaccgac gcccgtggcg cagctcgccc gctcccttga cctccccttg
      721 cgccagcccg cccgcatgtc cgaggtggcc ggggagatat cctctaacga cgccctcgtc
      781 gtcgccgcct acggccagat actgcgcccc gatactctgt acgcggccaa cgaaggcgcc
      841 tggaacgtcc acgcttctct cctgccgaag taccgcggcg ccgccccgat cgaacggtcc
      901 ataatggcag gcgagcccga gagcggcgtc accataatcc gtatggacga gggactggat
      961 accggcccca tggccctgca gcgcccactc cccatccctc cagacatgac cggcggcgaa
     1021 ctctccgagc tcctcgcctg cctgggagct gaagctgtag ttgaggttct aaatcagatc
     1081 gaatctggca cggtaaacct catcgagcag gacagtcttc atgcaactta tgcacctaaa
     1141 ctactggatg aggatctgga gatccggtgg gacaggagtg tggaggacgt agcgaatttg
     1201 gtgcgtgcgc tctcgccaca cgtaggggcg agggcgttca attccggggt agaggggcct
     1261 gtcaagatct ggcgggccga ggtctttgat ggagaggtac ccaccctgaa agcgggcgca
     1321 atacggacgg agaacggccg gatcctcgtg ggctgcggcg tcggggtcct cgaggtgctg
     1381 gagttgcaga tgcccggagg gaagcggctc gcggcccgcg acttcctgcg aggcaatacc
     1441 ctcgaaggcg cgttcgaccg ctagggcctc gccggcaagc gcgcccggcc aggctgcggc
     1501 caggctatgc cacagatgca gaaatgagcg caatatgcca gaatgggtgc taaactgccg
     1561 ggagtaatac ggaaggatct tacggggaga agatcgacat aagagaaccc gaggcagcca
     1621 cttctacgcc tgaaccggaa gtcgagatgc agcgtatcga cctcgacgaa gaaataaggg
     1681 tagctatagt cggcgctgga ggatacgggc gggttgcttt ggacgtcttg ctggctagcg
     1741 ggctcggcga cagggttctg gggttctacg acgacgctca cgccgcactt tcgggggagg
     1801 tgcggggcgt tccgatactt ggtgacatcg gcatgctcaa gagcatgctc tccgtcgagc
     1861 ctgtgcacgt cgtcgtggcg ataacggaca acgggacgcg gctacgggtc gccaactcgc
     1921 tgcgggctct gggcgcgcgg tttatcacca cgctccaccc tgacgcgtac ctctccggtg
     1981 cggccttggt tggcgacggg tgcgtgctgg cggctggggc tgtcgtgcac ccggacgcgg
     2041 ctgtggggag ccactgtttc gtcgggccgg gggctgttgt ggaccgggac gcggaggtgg
     2101 gggcggggac ctggctctcc gccgggtgcg tcgtggggcc gggagcccgc gtcggggcgc
     2161 gggtcgtcct cggccagaac tccagcgtcg gccgcaaggc ggtcatcgtt ggcgacgcaa
     2221 aggtcggtgc gttgcggcac gttgatcggg aggacgcttg accttaccgg cgcagctccg
     2281 aggagcttgt ccgggttcga tccggggggt gtgggggtca tggaccgcct gggcggctta
     2341 ggacgagcgg gcgcccggac catcgggctg gacgtagacc gcggcgccct caaggcggtc
     2401 cagatctcgg ggagcgctgg tgagctcacg ttgcggcacg tcgggtacca caggctgccc
     2461 cccggtgcgg tagtcgacgg cgaggtcgcc gatgtagagg tcctcggtgc ggagatccgg
     2521 gagttctggt cctcgcactc tttcaagggg aagtcggtca acctcggcgt cgcgaacggc
     2581 agggtggtcg tgcggctgct cgacttcccg cgcatggaag cagaagatct caagagcgcc
     2641 ataaccttcg aggcccagga tcacatcccc atgcctctgg atgaggctgt cctcgactac
     2701 gttgtgctcg gtccgcagtc cgagggttcc gacctggatc gcatactcgt cgtggccgcg
     2761 cacaaggaca tgatcggggg ctacacggcc gcgttgcggg cgggcgggct caagcccgca
     2821 gggatagatg tcaaggcgct cgcgctaacg cgctccg
//
LOCUS       DASTUV010000207         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_261250, whole genome shotgun sequence.
ACCESSION   DASTUV010000207 DASTUV010000000
VERSION     DASTUV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261250"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1560)
                     /gene="murA"
                     /locus_tag="VFT03_04185"
     CDS             complement(271..1560)
                     /gene="murA"
                     /locus_tag="VFT03_04185"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4847373.1"
                     /translation="MERFLVEGGARLHGEVEISGAKNAALKLIAASLLAPGTTTLRNV
                     PCIKDVDTMLAVLEGLGADAYFHGHTLEIDASDIDSYCAPYELVRQMRASIVVLGPLA
                     ARFGEAEVSAPGGCNLGLRKFNFHLDGLRALGAEVELDHGFIKATAPGGLRGAEVNFE
                     YPSVTATENVMMAAVLADGITIIENGAREPEVVDLAEFLNSMGASVNGAGTGRIVIEG
                     VEELEPVEWSVMPDRIEAGTFIMATAATGGDVEIKTARRDHLKMELEKLREMGVEITD
                     LDDPAGGVRVRVDDPADLSGVDVSTLPFPGFATDLQAQMMVALTQATGAGIITENVYE
                     NRLQVAEELNRMNAGIDLFGGHRALVKGPRTLSGTIVQAPDLRGGAALVLAGLVAEGT
                     TIVDGARHILRGYEDFEEKLSRIGATVAFEAGTPAVP"
     gene            complement(1615..2133)
                     /gene="atpC"
                     /locus_tag="VFT03_04190"
     CDS             complement(1615..2133)
                     /gene="atpC"
                     /locus_tag="VFT03_04190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEU4847374.1"
                     /translation="MTEAREERREGRQLFCRIITPERPVFDGEADLVITRIADGDLGV
                     LVDHAPTVSTVEIGEVRIHQGEERSIYATSDGFFKVSENLVQILVEEAVPPEEIDVED
                     AENRVEEAERELAEVSDDDERRKEVMRRQRVAENLVRVARRYEGEGSPGRLRGCNANV
                     MRLLPLRNLLQS"
     gene            complement(2130..>3512)
                     /gene="atpD"
                     /locus_tag="VFT03_04195"
     CDS             complement(2130..>3512)
                     /gene="atpD"
                     /locus_tag="VFT03_04195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU4847375.1"
                     /translation="DVQDTGSPITVPVGEQVLGRVLDVLGNPVDEQGPIEDPEGHWSI
                     HRPSPRFEDLSTQAEQFITGIKVMDLLCPVRRGGKVGLFGGAGVGKTVVIQELINNIA
                     LQYEGTSVFAGVGERTREGNDLYGEFQEAGILGNVALVFGQMNEPPGARFRVGLTGVT
                     VSEYFRDEQGQDVLFFMDNVFRFVQAGNEVSALMGRMPSEVGYQPTLATEMGALQERI
                     TSTNKGSITSFQAVYVPADDFTDPAPFTVFAHLDSTVELARNLAEQGIYPAVDPLTSS
                     STILDPSVVGEEHYEVAQQVKNILQRYKELQDIIAILGIDELSEEDKVIVGRARRLQR
                     FLSQPFFVAEQFTGMEGKFVELEDTIRSFKEVVEGKHDELPEQAFYLVGNIDEAIEKA
                     RGMSGEEEEEGEEEGESEDQSEGEDESGEEPEGEEGSEEEDSGEEEEEAQSEDESEGD
                     GESEEGDERR"
BASE COUNT          564 a         1196 c         1081 g          671 t
ORIGIN      
        1 catctgtcag ccagacccct cgtactatag tagacggcac ctctttcggc ggaggaagcg
       61 agggtttttg gcagcaatct cctcgagcgc actcccttct cactctgtgt tggggcggag
      121 cgaaaagaat tcgcgccgta cgacgttaca ggagcagcat gcgccagggg gagggagagc
      181 acatacccca aatggatgtt ctttgccgtt agcagagagc tgcttgcgtg ttacgaatcg
      241 tctgcaagat cgaccgacgg ttgccggttc tcaggggacg gcaggggtgc cggcctcgaa
      301 ggctacggtc gccccgatcc gggagagctt ctcctcgaaa tcctcgtagc cgcgcagaat
      361 gtggcgagct ccgtccacta tcgtggtgcc ctcggcgacc aggccagcca gcaccagcgc
      421 agccccgccc cgcaggtccg gggcctgcac aatggttccg gagagagttc tgggaccctt
      481 gacgagggcg cggtggccgc cgaagaggtc tatgcccgcg ttcatccggt tgagctcttc
      541 ggccacctgg agcctgttct cgtagacgtt ctcggtgatg atccccgccc ccgtagcctg
      601 ggtgagggcg accatcatct gagcctgcag gtccgtcgcg aagccgggga acgggagggt
      661 cgagacgtct accccgctga ggtcagcggg gtcatccacg cgcacgcgga ccccgcccgc
      721 ggggtcgtcc aggtcggtga tctccacacc catctcacgc agcttctcca gctccatctt
      781 gagatgatcc cggcgggccg tcttgatctc cacgtcccca cctgtggcgg cggtcgccat
      841 gatgaaggtg ccggcctcga tgcggtccgg catcaccgac cattcgacgg gctccagctc
      901 ctcgacgccc tcgatgacga tgcggcccgt ccccgccccg ttgacgctcg cgcccatcga
      961 gttaaggaac tccgccaggt cgacgacctc cggctcgcgc gcgccgttct cgatgatcgt
     1021 gatcccatct gccaggacag cagccatcat cacgttctcc gtggcggtga cgctcgggta
     1081 ctcgaagttc acctccgcgc ccctgagccc ccccggcgcc gtcgccttga tgaagccgtg
     1141 atccaactcg acctccgcac ccagagccct gaggccatcg aggtggaagt tgaacttgcg
     1201 caggcccagg ttgcacccgc ccggcgcgga gacctcggcc tcgccgaagc gggcggcgag
     1261 cggtccgagg accacgatgg aggcccgcat ctggcgcacc agctcgtagg gagcgcagta
     1321 ggagtcgata tccgaggcgt ctatctcgag ggtgtgcccg tgaaagtagg cgtcggcccc
     1381 caggccttcg aggaccgcga gcatcgtgtc gacgtccttg atgcaaggca cgttgcgcag
     1441 ggtagtcgtg cctggcgcca atagagacgc ggcgatgagc ttcagggccg cgttcttcgc
     1501 cccggagatc tccacctcgc cgtggagccg tgctcctcct tcgacgagaa aacgctccat
     1561 ttccaccctc cccgatacag catcgataca gaccagatcg gttgtgcaaa gatactatga
     1621 ctgaagcagg ttacgcaacg gtaacagcct cataacgttt gcgttacagc cgcgaaggcg
     1681 gcctggcgaa ccctctccct cgtacctccg cgcaacacgg accaggttct cggccacgcg
     1741 ctggcggcgc atgacctctt ttcgcctctc gtcgtcgtcc gagacctctg ctagctccct
     1801 ctcagcctcc tcgacgcggt tctcggcgtc ctcgacatct atctcctccg gcggaacggc
     1861 ctcctcgacc aggatctgga ccaggttctc ggagaccttg aagaaaccgt cggaggtggc
     1921 gtagatgctc cgttcctcgc cctggtggat gcggacctcc ccgatctcga ccgtcgagac
     1981 ggtcggggcg tggtcgacca ggaccccgag gtcgccatcc gctatcctcg tgatgacgag
     2041 gtcggcctcg ccgtcgaaaa caggccgctc aggcgtgatg atccggcaga agagctgccg
     2101 gccctcgcgc cgctcctcgc gggcctcagt caccgcctct cgtcgccttc ctcagactcg
     2161 ccgtctcctt cagattcgtc ttcggactgg gcctcctctt cttcctcgcc ggagtcctct
     2221 tcctcagagc cctcttcccc ttcgggctcc tcgccggact cgtcctcccc ttcggactgg
     2281 tcttcggact cgccctcttc ttctccttct tcctcttcct cgccgctcat cccgcgggcc
     2341 ttctctatag cctcgtcgat gttaccgacg aggtagaagg cctgctccgg cagctcgtcg
     2401 tgcttacctt cgacgacctc cttgaacgac cggatcgtgt cttcgagctc gacgaacttg
     2461 ccctccatgc cggtgaactg ttcggcgacg aagaacggct gcgagaggaa gcgctggagc
     2521 ctcctcgccc ggcccacgat gaccttgtcc tcctcggaga gctcgtctat gccgaggatg
     2581 gcgatgatat cctggagctc cttgtagcgc tggagaatat tcttgacctg ttgggcgacc
     2641 tcgtagtgct cttcgcctac gaccgagggg tcgaggatcg tcgaggatga ggtcagcggg
     2701 tcgaccgccg ggtagatgcc ctgctcggcc aggttgcgcg cgagctcgac cgtcgagtcg
     2761 aggtgggcga acacggtgaa cggggccggg tcggtgaagt cgtcggcggg gacgtagacg
     2821 gcctggaagc tcgttatcga gcccttattg gttgaggtga tgcgctcctg gagcgccccc
     2881 atctcggtgg cgagggtggg ctggtagccg acctccgacg gcatcctgcc cattagcgcc
     2941 gagacctcgt tgcccgcctg cacgaagcgg aagacgttgt ccataaagaa cagcacgtcc
     3001 tgcccctgct cgtcgcggaa gtactccgat acggttacgc cggtgaggcc gacgcggaag
     3061 cgggcgcccg gcggctcgtt catctggccg aagacgagcg caacgttacc gaggatgccg
     3121 gcctcctgga actcgccata gagatcgttc ccctcgcgcg tgcgctcgcc gacgccagcg
     3181 aagacgctcg tgccctcgta ctgcagggcg atgttgttga tcaactcctg tataacgacg
     3241 gtcttgccga cgccggcgcc gccgaagagg ccgaccttgc cgccgcgcct gaccgggcag
     3301 agcaggtcca tgaccttgat gccggtgatg aactgctccg cctgggtcga gaggtcctcg
     3361 aaccgtggcg aggggcggtg gatcgaccag tggccttccg ggtcctcgat agggccctgc
     3421 tcgtccaccg ggttgccgag gacgtcgaga actcgcccga ggacctgctc gcccacgggc
     3481 accgttatcg gactgcccgt gtcctgcacg tc
//
LOCUS       DASTUV010000208         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_58591, whole genome shotgun sequence.
ACCESSION   DASTUV010000208 DASTUV010000000
VERSION     DASTUV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58591"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /gene="aroC"
                     /locus_tag="VFT03_04200"
     CDS             <1..1160
                     /gene="aroC"
                     /locus_tag="VFT03_04200"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU4847376.1"
                     /translation="FSTAGESHGPGGVVIVHGVPAGLGLVAGDVDRDLARRQKGYGRG
                     GRQKIEKDEVEFLGGVRHGYTLGSPVAMLIRNDDYANWRDRMSPEPVEKAPEPITLPR
                     PGHADLAGMQKYAFDDLRNVLERSSARETAARVAAGGVAKKLLAEFGVRVESAVYRIG
                     SVAYPKERAALEAAKADESEVRCPDPEMSERMKAEIDAARHARDALGGEFVVVAEGCP
                     PGLGSYVDWRDKLDARLAAAVMSINAIKGVEFGMGFDLAAHRSSEVQDEILPGEDHPA
                     RASNRLGGIEGGMTNGEPVVVAAAMKPISTISRALRTVDLSTGEPARAFKERADSCAV
                     PAAAVIGESMVAVVLAEAFLEKFGADTMTDIRAAYDLYTERIKATGRGECS"
     gene            1165..1668
                     /locus_tag="VFT03_04205"
     CDS             1165..1668
                     /locus_tag="VFT03_04205"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU4847377.1"
                     /translation="MDGPVAIVGYMGSGKTTIGRVLARSLGWEFVDLDREISHRSRRA
                     IPEIFETSGEEAFRDMEHLALLAALDGTPERIVACGGGIVERPENRERLAKVPTVFLR
                     EEVGILFGRTRDARRPLRAASREGFDRRYERRLPLYEEVANLEIVLNSRPPQAVAEEI
                     ERWISDA"
     gene            1650..>2515
                     /locus_tag="VFT03_04210"
     CDS             1650..>2515
                     /locus_tag="VFT03_04210"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEU4847378.1"
                     /translation="MDLGRVEVPVAPPYPVVVGAGLDLAEVVSTELEPGLAAIITDSN
                     VGPLHAPQVSGALEDAGWRVAEVLQVLAGEASKGLAAYAEVAGRLARAGMTRDGTLFA
                     LGGGVVGDLGGFVAATYMRGIGLVALPTSLLAMVDSSVGGKVGVDLPEGKNLLGSFLR
                     PRLVAADLDWLETLPGRELSRGLAEVIKMGLLSGGRYFEDLDGVGAARSRDPDALRAL
                     ILDSVRFKAQVVAEDELEAGRRAILNYGHTIGHGLEAATGYALPHGEAVAAGMVAAAH
                     IARERLSVDLVSL"
BASE COUNT          397 a          823 c          910 g          385 t
ORIGIN      
        1 gcttttcgac ggcgggtgag tcgcacggcc ccggcggggt ggtcatcgtg cacggggtgc
       61 cggctggcct cgggctcgtc gccggggacg tggacaggga cctggcccgc aggcagaagg
      121 gctacggtcg gggcggccgg cagaagatag agaaggacga ggtggagttc cttggcggcg
      181 tgcgccacgg ctacactctg ggctctcccg tcgccatgct catccgcaac gacgattacg
      241 cgaactggcg ggacaggatg tcgccggagc cggtcgagaa ggcgccagaa cccatcacgc
      301 tgccgcgtcc gggtcacgcc gacctggccg ggatgcagaa gtacgccttc gacgacctgc
      361 gcaacgtcct cgagcgctcc agcgcccgcg agaccgcggc gagggtcgcg gcaggcgggg
      421 tcgcgaagaa gctcctcgcc gagttcggcg tgagggtaga gagcgcggtc taccgcatag
      481 ggagcgtcgc gtatcccaaa gagcgcgctg cgctcgaggc cgccaaggcc gacgagtcgg
      541 aggtgcgctg tcccgacccc gagatgtccg agaggatgaa ggctgagata gacgcggccc
      601 gtcacgcccg tgacgccctg ggtggagagt tcgtcgtcgt agccgagggg tgcccgccgg
      661 ggctgggctc ttacgtcgac tggcgcgaca agctcgacgc gaggctcgcc gccgccgtga
      721 tgtccataaa cgccataaag ggcgtcgagt tcgggatggg cttcgacctg gcagcccatc
      781 gctccagcga ggtgcaggat gagatcctcc ccggcgaaga ccaccccgcg cgcgccagca
      841 accgcctcgg cgggatcgag ggcggtatga ccaacggaga gcccgtcgtc gtggcggctg
      901 cgatgaagcc gatctcgacc atctctagag cattgcgcac cgtcgacctc tcgaccggcg
      961 agcccgcgcg ggccttcaaa gagcgggcgg attcttgcgc cgtgccggct gcggccgtga
     1021 tcggggagtc catggtcgcc gtagtcctcg ccgaggcctt cctggagaag ttcggcgcgg
     1081 acaccatgac ggacatccgc gccgcctacg acctctacac cgagcgtata aaagcgaccg
     1141 gccgcgggga gtgcagctga aagcttggat ggacccgtcg ctatcgtcgg ctacatgggt
     1201 agtggtaaga ccacgatcgg acgcgtgctc gcccgctccc tcggctggga gttcgttgac
     1261 ctcgaccgcg agatctcaca ccgctcccgg cgcgcgatac cggagatctt cgagacttcc
     1321 ggcgaggagg cgtttcgtga tatggagcac ctggcgctcc tcgcggccct cgacgggacg
     1381 cccgagcgga tcgtggcctg cggtggcggg atcgtcgagc gccccgaaaa ccgcgagcgg
     1441 ctcgcgaaag tcccgacggt ctttctgcgg gaggaggttg gtatcctgtt cggtaggacc
     1501 agggacgcca ggcgccccct cagggcggcg agcagggagg ggttcgaccg tcgctacgag
     1561 cggcgtctcc ctctctacga ggaggtcgcg aacctcgaga tcgttctcaa cagtcgcccg
     1621 ccgcaagcgg tggcggagga gatagagcgg tggatctcgg acgcgtagag gtccccgtcg
     1681 ccccacccta ccctgtcgtc gtcggcgcgg gtctcgacct cgccgaagtc gtctccacgg
     1741 agctggagcc cggcctcgcg gcgatcatca ccgactccaa cgtcgggccg ctgcacgctc
     1801 cacaggtatc tggagccctc gaggacgctg gctggcgcgt ggcggaggtg cttcaagtcc
     1861 tggccgggga ggcatcgaag ggtctggctg cgtacgcgga ggttgccggg aggctcgccc
     1921 gcgcgggcat gacgcgcgat gggacgctct tcgcgctcgg gggcggtgtg gtgggagatc
     1981 tcggcggctt cgtggcggct acctacatgc gcgggatagg cctggtcgcg cttcccacct
     2041 cgcttctggc catggtggac agctccgtgg gcgggaaggt cggcgtcgac ctccccgagg
     2101 gcaagaacct tctagggagc ttcctgcggc cccgcctggt cgcggccgac ctcgactggc
     2161 tcgagacgct cccaggacgt gagctctccc gcggactagc cgaggtgatc aagatgggtc
     2221 tactctccgg cggacgatac ttcgaggacc tcgacggtgt cggggcggcg aggtccaggg
     2281 acccggacgc cctgcgcgcc ctgatcctgg actccgtccg cttcaaggcg caggtcgtgg
     2341 ccgaagacga gctggaagcg ggacggcgcg ccatcctgaa ctacggccac accatcggtc
     2401 acggcctgga ggccgctaca ggctacgccc tgccacacgg tgaggctgtc gccgcgggga
     2461 tggtcgccgc cgcccacatc gcccgcgaga ggctgagcgt cgacctcgta agcct
//
LOCUS       DASTUV010000209         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1722373, whole genome shotgun sequence.
ACCESSION   DASTUV010000209 DASTUV010000000
VERSION     DASTUV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722373"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1691)
                     /locus_tag="VFT03_04215"
     CDS             complement(240..1691)
                     /locus_tag="VFT03_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4847379.1"
                     /translation="MSTETAQSYPAIIGGEKVDAAQTFEDLDPSTGQPCAQVARCGEG
                     EIDQAVEAALRTHEDTWRRTIPAERARILHRIAELLRRGHEEIARLESMDTGKPLTQA
                     RADATVAARYFEFYANTIESFYGDTIPALEGTFVYTLREPYGVTAHIIPWNYPIQISS
                     RSVAPALAAGNCCVVKPAEEAPLTAIRLAELALEAGLPPGALNVVPGYGEEAGAALAA
                     HPGIDHLAFTGSREVGQIVSKAAADNVIPVTLELGGKSPNVVFADADVESAAPVIVNS
                     IIQNAGQTCSAGSRLLVQKEIHDQVVETITELFGRITIGPGLDDPDLGPIISGDQRER
                     VQGYVDRGKEEARLVFGGYVPQDEGLRGGFFFEPTLFDDVSPDAVIAQKEVFGPVLVA
                     STFDDLGEATAVANGTDYGLIAAVWTRDVGKAHWLANELRAGQVYVNTYGAGGGVELP
                     FGGFKKSGHGREKGYEALYEYSQVKTVALKYGP"
     gene            complement(1704..2804)
                     /locus_tag="VFT03_04220"
     CDS             complement(1704..2804)
                     /locus_tag="VFT03_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU4847380.1"
                     /translation="MGRVKGKVAALVGPAEVAVKEFEVPEPEPGAVLVKIRRANVCGS
                     EVHIYHFRHPLIRECVLGHEFVGEVLALGEGVTTDYAGNPVEVGDRVIAPYFLTCRRC
                     PACLRGDFNLCQKAYTFWSRPPEDDPHFHGAFATHYYVHPEQYFYKVPDSIPDNTVAG
                     ANCGLSQVLFGLHQTGLSTGDSLLIQGAGGLGLYAAAIGRDMGAQVIVVEGIRQRIDL
                     ARRFGAEEIVNMKEHPEAGDRVRQVHSLTDGYGADVVLEVAGVPAAFVEALQLVRPGG
                     RVIEIGNVSVGKEHEVSLAPGLITRKMIRVQGFVRYQPWYLHRALGFLERKHDAHPFD
                     ELTDEEYALKDVGRAIERAEAKKVARPAVVPG"
     gene            3102..>3402
                     /locus_tag="VFT03_04225"
     CDS             3102..>3402
                     /locus_tag="VFT03_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU4847381.1"
                     /translation="MPYIRVATFDGPGEPPVIREVPKPEVPSKAALIRIAACGVCGTD
                     LHILKGHWPRPLPWPFTLGHELVGVVEEIGEELTEDFMGQPLEIGSKVMLPPLMPC"
BASE COUNT          587 a         1152 c         1003 g          660 t
ORIGIN      
        1 ctgttaccta tacataccta cccacaaacc taccccacct ggatgaagtc tatggcctcg
       61 gatgcttcga gtctgtcggt gtactgggcg aagttgacgg tccgcgacag tcttcgagct
      121 cgaacgaagg cgcgacccca ccagaacggt gccctgctgc cgtctgggcg agcgtgactc
      181 gtcggtcaga cgctaccccg cggacggttc actacgacta tccgctcggt gcaggtcagt
      241 tacggaccgt atttgagcgc tacggtcttt acctggctat actcgtagag ggcctcgtat
      301 cccttctccc gaccgtgccc ggactttttg aaccccccga acggaagctc aacacctccg
      361 cccgccccgt aggtgttgac gtacacctgg ccggcgcgta gctcgttggc gagccagtga
      421 gccttgccta cgtcgcgcgt ccagactgcg gcgataaggc catagtccgt gccgttggct
      481 accgcggtgg cttcgccgag gtcgtcaaag gtagaggcga cgagcacagg gccgaagacc
      541 tccttctgag ctatcaccgc gtccggcgaa acgtcgtcga agagggtggg ctcgaagaag
      601 aagccgcctc tcaagccttc gtcttgaggc acgtagccgc cgaacaccag ccgcgcctcc
      661 tccttgccac ggtcgacgta cccctgcacg cgctcccgct ggtctccgga gatgatcgga
      721 ccgaggtccg ggtcgtcgag gccgggacca atggtgatcc gcccgaacag ctcggttatc
      781 gtctctacta cctggtcgtg gatctccttc tgcacgagga gccgcgagcc ggcggagcag
      841 gtctgccccg cgttctggat gatggagttg acgataacgg gagcggcgct ctcaacgtcg
      901 gcatccgcga agacgacgtt cggcgacttg ccgccaagct ccagggtcac ggggatgacg
      961 ttatcggcgg cagccttact tacgatctgt cctacctccc gcgaacccgt gaaggcgagg
     1021 tggtctatac cagggtgcgc ggccagcgcc gcgcccgcct cctcgccgta gccgggcacg
     1081 acgttcagcg ctcccggggg caggccggcc tccagcgcga gctcggcgag cctaatggcg
     1141 gtgagcggcg cctcctcggc cggcttcacc acgcagcagt tgcccgctgc cagggcagga
     1201 gcaacggagc ggctgctgat ctggatcggg tagttccatg ggatgatatg ggccgtgacg
     1261 ccgtagggct cgcgtagggt gtacacgaac gtcccctcca gggccggtat cgtatcccca
     1321 tagaacgact cgatggtgtt ggcgtagaac tcgaagtacc gggcggcaac cgtggcgtcg
     1381 gcccgcgcct gcgtgagcgg cttgccggtg tccatgctct cgagccgggc aatctcctcg
     1441 tggccccgcc gcaacaactc ggcgattctg tgcaggatcc tggcccgctc agccggaatg
     1501 gtgcggcgcc aggtatcctc gtgcgttctc agtgccgcct ctactgcttg gtcgatctcc
     1561 ccctcgccac accgcgctac ctgcgcgcag ggctgcccgg tcgatgggtc cagatcctcg
     1621 aacgtctggg ccgcgtccac tttctcccca ccgataatgg cgggatagct ctgtgctgtt
     1681 tcggtgctca tctgctctcc tctctaaccc ggcacgaccg ccggacgcgc cactttcttc
     1741 gcctccgccc gctcgatggc cctaccgaca tccttcaggg cgtattcctc atcggtcagt
     1801 tcgtcgaagg gatgggcgtc gtgcttgcgt tccaggaacc ccagagccct gtgcagatac
     1861 cacggttggt agcgtacgaa accctggacg cgtatcatct tccgggttat gaggcccggg
     1921 gccagggata cctcgtgttc cttgccgacg cttacgttgc cgatctcgat gacccttccg
     1981 ccgggccgta cgagttgcaa ggcctccacg aatgccgccg gaacgcccgc aacttccagg
     2041 acaacgtccg cgccgtagcc gtcggtcagc gagtgcacct gccgcaccct atctccggcc
     2101 tctggatgct ctttcatatt tacgatctct tcagccccga accgccgggc gagatcgatg
     2161 cgttgccgta tcccctcgac tactataacc tgagccccca tatccctacc gatcgccgcc
     2221 gcgtagagac cgaggccacc ggcgccctgg atcaggaggc tgtcaccggt tgagagccct
     2281 gtctggtgca gcccgaacag gacctgcgag aggccgcagt tcgcccccgc tactgtgttg
     2341 tccggaatgc tatccggtac cttgtagaag tattgctccg ggtgcacata gtagtgcgtg
     2401 gcgaaagccc cgtggaagtg tggatcatct tccgggggcc tggaccagaa cgtgtatgcc
     2461 ttctggcaga gattgaagtc gcctcgcagg cacgccggac atctccgaca cgtcaggaag
     2521 tacggcgcga tcacccggtc gcctacctcc accgggttcc ccgcgtagtc cgtggttacg
     2581 ccttcgccaa gggctagtac ttccccgacg aactcgtgcc ccaggacgca ctcgcggatg
     2641 agcgggtgac ggaagtgata gatgtgcacc tctgacccgc aaacgttcgc ccggcggatc
     2701 ttcacgagca ccgccccggg ctccggttcc ggtacctcga actccttgac cgcaacctcc
     2761 gccggcccca ccagtgccgc aaccttccct ttcaccctac ccatctcctt ccccataact
     2821 tcatccgtgc gaaggatgca tagaataata catctctacc gtttgcatcc cgagccttag
     2881 tgacaatgga tctccgaacg cacccaaagg taggtgtgac ccgatcctgg accagatgct
     2941 cgaagcacac ttctcgcgac tcatcgtcgc caggcaggac atacagcacc gaagcagacc
     3001 gcttgaccgg gttgtaaccg atgtgtattt tccttcgtat agccgtagcc gctaggggca
     3061 tgacagccga tgcgcccttg cgcttgccat taggaggacc catgccgtac atccgcgtcg
     3121 ccaccttcga cggccccggt gagccgcccg ttatacgtga ggtgccaaag cccgaggtgc
     3181 cgtccaaggc tgcgctcatc cgcatcgcgg cctgcggggt ctgcggcacc gacctccaca
     3241 ttctcaaggg ccactggcct cggccgctac cctggccgtt cacactaggg cacgaattgg
     3301 taggggttgt ggaagagatc ggggaggagc tgaccgagga cttcatgggc cagccgctcg
     3361 aaatagggtc caaggtcatg ctgccgccgc ttatgccctg cg
//
LOCUS       DASTUV010000210         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_614545, whole genome shotgun sequence.
ACCESSION   DASTUV010000210 DASTUV010000000
VERSION     DASTUV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614545"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFT03_04230"
     CDS             <1..1191
                     /locus_tag="VFT03_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEU4847382.1"
                     /translation="LERTHNGRRAVYFDGPGGSQVAKSAIDAVSGYMSRGGANLHGPF
                     PTSVETEEILRDTRQAMADFLGAGPDEVAFGANMTTLTFAVSRALARNWDRNSEVVVT
                     ELDHRANVDPWLLAAAERGAAVRWVRVNPETLTLEEDDIERAINDRTRLVAVGLASNA
                     VGTVNDVAAIAKMAHGAGALVAVDAVHAAPHIAVDRDAIGADVLTCSAYKFFGPHVGV
                     TAVRRNLLETLDVYKVEPAPDYIPDKLETGTQNHEGIAGAKGAIDFISSLGHGTSLQD
                     KLVSGMRAIEEYEADLAERFRAALHEIPEVKLYAAPDGVLKTPTVAFRVEGHTPREVT
                     ERLAQEGFFVADGHFYASTLARKLGIHDKGGWIRAGLAPYNTEEEVEGFIESLKSLVK
                     HNRS"
     gene            1563..1766
                     /locus_tag="VFT03_04235"
     CDS             1563..1766
                     /locus_tag="VFT03_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU4847383.1"
                     /translation="MAQGTVKWFSDEKGYGFISPDDGGEDLFVHHTGIAGSGFKSLEE
                     GQKVTYEATQGRKGMQAENVTPA"
BASE COUNT          447 a          661 c          684 g          359 t
ORIGIN      
        1 cttgagagga cccacaacgg gcgcagagcc gtatacttcg acggacccgg cggctcacag
       61 gttgcgaagt cagccatcga cgcggtatcg ggctacatga gccgtggcgg agccaacctg
      121 cacggcccct tccctacgag cgtcgagacc gaggagatcc tgcgcgacac ccgtcaggct
      181 atggccgact tcctgggcgc agggccggac gaggtggcct tcggggcgaa catgaccacg
      241 ctcacgttcg cggtatcgcg cgcgctggct cgcaattggg acaggaattc cgaggtcgtg
      301 gtgaccgagc tcgaccaccg cgcgaacgtg gacccctggc tcctcgccgc cgccgagaga
      361 ggagccgcgg tgcgctgggt cagggtcaac cccgagacgc tcaccctgga agaggacgac
      421 atcgagcgtg cgatcaacga taggaccagg ctcgtagccg tcgggctcgc ctccaatgcc
      481 gtcggcacgg tcaacgacgt ggccgcgata gcgaagatgg cgcacggggc cggcgcgctc
      541 gtcgcggtgg acgccgtgca cgccgctccc cacatcgctg tagaccgcga cgccataggc
      601 gccgacgtac tcacctgctc cgcctacaaa ttcttcggcc cgcacgtcgg cgtgacggcc
      661 gtaaggcgta acctcctcga aaccctggac gtttacaagg tcgagcccgc gccggactac
      721 ataccggaca agctcgagac cggcacccag aaccacgagg gcatagccgg cgcgaagggc
      781 gcgatagatt tcatctcctc tctgggtcat ggaacatctc tacaagacaa actcgtctcc
      841 gggatgcggg ccatcgagga gtacgaggcg gatctggccg agaggttccg cgccgcgctg
      901 cacgagatcc ccgaggtaaa gctctacgct gcccctgacg gcgtcctcaa gaccccgaca
      961 gtcgcgttcc gggtagaagg acacaccccg agggaggtaa cagagcgctt ggcgcaggag
     1021 gggttcttcg tcgccgacgg ccacttctac gcctcgacgc tggcgaggaa actggggatc
     1081 cacgacaagg gcggttggat ccgcgcgggc ctcgccccgt acaacaccga agaagaggtc
     1141 gaagggttca tcgagtcgtt gaagagcctg gtgaagcaca accgctcctg agcctgtgcc
     1201 ggaccagaga tccaggagaa tcctggcacc ccgttacgtc cggatcggga ggtgccgccg
     1261 ttgggctgta tctcgcgggc gagagtcttg ccgttgccgc aatccacgtt tgcgacatag
     1321 gtaggttgga tcacgactct gttagaaagg cactagaggt cactgatgta actctctaac
     1381 catggaaaag ggggcacggc ccattctcag cgacattagc gagcccgagt tgcgcctcgc
     1441 cgtaagatct ctgttatact gaagtcgcaa gttaaggtgg gttacagcag acggagcgcc
     1501 acccagcaag agagggtggc gtttatttgt tgggcccagg aacacgaaag gggctccagt
     1561 atatggccca gggaacagtg aagtggttca gcgacgagaa gggctacggc tttatctccc
     1621 cagacgatgg gggtgaggat ctcttcgtgc accacacggg tatagccggc agcggcttca
     1681 agtccctcga ggaaggtcag aaggtgacct acgaggcgac ccagggcagg aaggggatgc
     1741 aggccgagaa tgtaactcct gcttagagat ccagcaagca agaccgcgga agccggggct
     1801 cgttgccccg gcttcttttt tgcgacgaat tctacgaacc ctgacccggc agcaggtaaa
     1861 agcagcgcct tctgggagtg tagccggttc gaggccgcga gcctcagcag ctaatggtta
     1921 ccgcgtgcct ggtcaacttc gccaagctac gggacaaatt cactactgcc ccttcgcaac
     1981 atccgcttct aatattggct acaggtgcta gcggaaagta ggattgttcc gatgccggac
     2041 gtgtgggcca cctttaccga gctagatact gccatgcagg aacgcctcgc cgacgtcctc
     2101 gagacgcgag gtgcccaccc gcgccagcag gcgatgcgcc gcgccttcct c
//
LOCUS       DASTUV010000211         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_733240, whole genome shotgun sequence.
ACCESSION   DASTUV010000211 DASTUV010000000
VERSION     DASTUV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733240"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFT03_04240"
     CDS             <1..619
                     /locus_tag="VFT03_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU4847384.1"
                     /translation="IVGGSMGIACGAAWAFKQRKEDRVAVCFFGDGAVQEGIFNETLN
                     MAAIWKLPVVFVCENNQYAMSLSVKRSFATERISDRASGYGMPGETVDGMELDQVREA
                     ASEAVDLARSGGGPTLIEAVTYRYLGHSKSDANLYRTRDEISEWRERDPIGRFATRLE
                     EEGAIEQDAWREIERRTRERVEAAFEKAAQEPEPEPETALEDVYA"
     gene            612..1625
                     /locus_tag="VFT03_04245"
     CDS             612..1625
                     /locus_tag="VFT03_04245"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU4847385.1"
                     /translation="MPREITYREAVREAMVQAMREDDSVFLMGEDVGVYGGAFGVSRG
                     MVEEFGEDRVVDTPLSEAGFVGLCVGAALMGMRPIAEIQFSDFITHSMDQLVNQAAKL
                     RYMFGGEGRVPVVVRTPGGAGTGAAAQHSQSLEAWFIHVPGLKVVMPSTPYDAKGLLR
                     AALDDPNPVIFYEHKLLYNRKGEVPEEPYRVPLGEAAVSREGGDVTLVAAGLVHRFAL
                     EAADSLADEGIEAEVVDLRTLSPMDHEAVARSIQKTGKLVVVEEDVKTAGWGAEVVAR
                     IVESESFYALDRAPARVAGADVPIPYNKNLEAYVRPSPEKVVEAVRSLLDARVGVYSE
                     VYS"
     gene            1626..2819
                     /locus_tag="VFT03_04250"
     CDS             1626..2819
                     /locus_tag="VFT03_04250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU4847386.1"
                     /translation="MATELKVPTLGMDMEEATIVRWLVEEGAEVEKGDPVLEIDTDKT
                     SFEIEAPSDGAIHALRGEAGDTLPVGATLAYVTAPGEDFLEPQEESHTEEVKPERTAE
                     VPKNQTVPVSADSEDGRKVRASPAARRAAADMGVSIESVSGSGPYGRVYLSDVLEMET
                     EATAEQAEQAPIDAARREPLSRIRSLGAERTARSFSEVPHFYLTRDMEADRLVELLER
                     LKTKMDPAPSVTELLVLAVSRTLRDHPRLNGRYAGGELEVHDDVNVRVAVATDEGLVA
                     PAIAKADTLSLRELVPKVKDLMRRARDHELSPEELSGGTFTISNLGMMGIDSFDAIIN
                     TPEAAILAVGRVRTVPEWRDGWWAPQRVISATLSVDHRVADGADGARFLADLQSVLSD
                     WELLL"
     gene            2833..3183
                     /locus_tag="VFT03_04255"
     CDS             2833..3183
                     /locus_tag="VFT03_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847387.1"
                     /translation="MKVEKDAILTCSTCGIEGPHELLYLSQHLSASRCVNCGTTRVYS
                     DHIYSEYARDLAERTTHLPGRVAWDVLRHPTRIVKWPFKAIRKPFGLLREVNQVTAFE
                     QSRHKAPTPGRLIR"
BASE COUNT          611 a         1058 c         1099 g          491 t
ORIGIN      
        1 catcgtcggc ggcagcatgg gcatagcctg cggggccgcc tgggccttca agcagcgcaa
       61 ggaggacagg gtggctgtct gcttcttcgg ggacggagcg gtccaggaag gcatcttcaa
      121 cgagacgctc aacatggccg ccatttggaa gctgcccgtc gtcttcgtgt gcgagaacaa
      181 ccagtacgcg atgtccctta gcgtcaagcg cagcttcgcc acagagcgca tctcggaccg
      241 tgcctccggc tacggcatgc ctggcgagac ggtagacggc atggagctgg accaggtccg
      301 cgaagccgcc tcagaagcgg tggacctcgc ccggagcggc ggaggaccta cgctcatcga
      361 ggccgtaaca taccgctacc tcgggcactc caagagcgac gcgaacctct accggacgcg
      421 agacgagatc tccgagtggc gcgagcggga ccccatagga cggttcgcca cgcggctcga
      481 agaggaaggc gccatcgagc aggacgcctg gcgggagata gagcggcgta ccagagagag
      541 agtcgaggcc gccttcgaga aggctgcaca ggagcccgag cccgagcccg agaccgcgct
      601 ggaggacgtc tatgcctagg gagatcacct accgcgaggc cgtccgcgag gcgatggtcc
      661 aggcgatgcg cgaggacgac tccgtcttcc tcatgggcga ggacgtcggg gtctacggcg
      721 gggcattcgg cgtgagccgc gggatggtcg aggagttcgg cgaggaccgc gtggtagaca
      781 cgcctctctc ggaggccggc ttcgtcggcc tgtgcgtcgg agcagccctc atgggcatgc
      841 gcccgatagc agagatccag ttctctgatt tcatcaccca ctcaatggac cagctcgtga
      901 accaggcggc caagctgcgc tacatgttcg gcggagaagg ccgggtgccc gtggtggttc
      961 gcactcccgg tggcgctggc accggcgccg cagcccagca ctcccagtcc ctcgaagcct
     1021 ggttcatcca cgtgcccggc ctcaaggtcg tcatgccgtc aaccccgtac gacgccaagg
     1081 ggctcctgcg cgctgccctg gacgacccca acccggtcat cttctacgag cacaagctcc
     1141 tctacaaccg caagggcgaa gtcccggagg agccgtaccg ggtaccgctc ggggaggccg
     1201 cggtgagccg cgagggcggg gacgtgacgc tcgttgctgc ggggctcgtc caccgcttcg
     1261 cgctcgaggc ggctgactct ctggcggacg aggggatcga ggccgaggtc gttgacctga
     1321 ggaccctctc acccatggac cacgaagcgg tcgcgcgttc gatccaaaag acaggcaagc
     1381 tcgtagtggt cgaagaagac gtcaagaccg cgggctgggg cgccgaggtg gtagcccgga
     1441 tcgtggagag cgagagcttc tacgcgctcg acagagcccc cgcccgggtc gccggcgccg
     1501 acgtcccgat cccgtacaac aagaacctcg aggcgtacgt ccggcctagc cccgagaagg
     1561 tggtcgaggc tgtacgctcg ctgctggacg cccgcgtggg cgtctactcc gaggtgtact
     1621 cctagatggc taccgagctc aaggtcccta cgctgggcat ggacatggag gaggccacga
     1681 tcgtccgctg gctggtggag gagggcgcag aggtagagaa gggggacccc gtcctcgaga
     1741 tagacacgga caagacctcc ttcgagatag aggctccctc cgacggggcc atccacgccc
     1801 tccgcggaga ggcaggcgac accctgcccg ttggcgccac gctcgcctac gtcaccgccc
     1861 ccggagagga tttcctcgag cctcaagagg aatcccacac tgaagaggtc aagccggaaa
     1921 ggacggcgga ggtcccgaag aaccagaccg tcccggtatc ggcggactct gaggacgggc
     1981 ggaaggtcag ggcctcgccg gccgcccggc gggcagcggc tgatatggga gtctctatcg
     2041 agagcgtctc cggctcgggg ccgtacggca gggtctacct ctcggacgtc ctggagatgg
     2101 aaaccgaagc taccgctgaa caggccgaac aagcgcctat cgatgcggcc cgccgcgagc
     2161 cgctctcgcg cataagaagt ctcggggccg aacgcacggc gaggagcttc tccgaggtgc
     2221 cgcacttcta tcttacgcgg gacatggagg cggacagact ggtggagttg ctcgagcgcc
     2281 tcaagaccaa gatggacccc gcgccctcag tgacggagtt gctcgtcctg gcggtctccc
     2341 ggacgctcag ggatcacccg cgcctcaacg ggcgctacgc cggcggcgag ctcgaggtcc
     2401 acgacgacgt gaacgtcagg gtagccgtcg ccacggacga ggggctcgtg gcgccagcta
     2461 tcgcgaaggc tgataccctc tctctgcgtg aactggttcc gaaggtcaaa gacctgatga
     2521 ggcgggcccg agatcacgag ctctcccccg aggagctctc gggcggcacc ttcaccatct
     2581 ccaacctcgg gatgatgggc atagactcct tcgacgccat cataaacacc cccgaggcgg
     2641 ccatactggc cgtcgggcgc gtcaggacgg tgccggagtg gcgggacggc tggtgggctc
     2701 cgcagcgcgt gatctcggct acactatccg tagatcacag ggtagctgac ggggccgacg
     2761 gggcccggtt cctggccgat ctgcagtccg tgctctccga ctgggagctc ttgctgtgaa
     2821 gggggcgacg ctttgaaggt cgagaaagac gcaatactca cgtgctcgac gtgcgggatc
     2881 gagggccccc acgagctgct ctacctctcc cagcacctca gcgccagccg gtgcgtcaac
     2941 tgcggcacga ccagagtcta ctcggatcac atctactccg agtacgccag ggatctcgcc
     3001 gagcgcacca cgcacctgcc gggcagggtg gcctgggacg tgctgcgcca ccctaccagg
     3061 atagtgaagt ggcccttcaa ggccatccgt aaacccttcg gcctcctcag agaagtaaac
     3121 caggtcaccg ccttcgagca gagccgtcac aaagcgccaa cgccagggcg gctgattcgt
     3181 tagcacgctc aggcttcgcc ttcgcttgtc agccagtcag tgcagcgaga agctgagaag
     3241 ccgcctggcg aagccaggt
//
LOCUS       DASTUV010000212         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1568896, whole genome shotgun sequence.
ACCESSION   DASTUV010000212 DASTUV010000000
VERSION     DASTUV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568896"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFT03_04260"
     CDS             <1..477
                     /locus_tag="VFT03_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847388.1"
                     /translation="AFMSTPGLAEASAGAQLVDTLEHLFLPALTFTLGLIGEYALIMR
                     SSVIEAMNEDYVTTARAKGLRETLVLRKHVVPNALLPTVTLAALNLGFIVSGAITVET
                     VFSWPGLGQLTFRAINGGPDYPLLQGLFLLFSVAVIFANLAADLLLGVLDPRIGRA"
     gene            474..1379
                     /locus_tag="VFT03_04265"
     CDS             474..1379
                     /locus_tag="VFT03_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847389.1"
                     /translation="MSAGAAPSARAVRRTRRVRALKSSARLFAKNRMGVAGLAILTVF
                     VVLALFADVLVPEARLSVTEAPGAPLEPPSSQFWLGTDELGRSVLDLVIQGSQISLLV
                     GFLATAISMVIGSLVGISAGYFGGGIGALLMRFTDWFLVIPFLPLAIVLGAILGRSLF
                     VIALVIGLVTWAGTARVVRAQVLALKERPYVERARALGASNRQIIARHILPNVFPLIF
                     ANTILVVAVAILTETTLSFLGLGDPFSVSWGSILESAYAEGAISLGAWWYLVPPGVCV
                     VLVVLGFTMCGYAFDEILDPRLRDR"
     gene            1376..2365
                     /locus_tag="VFT03_04270"
     CDS             1376..2365
                     /locus_tag="VFT03_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4847390.1"
                     /translation="MSETLTPTGTLLEIKDLRVLYEVRGVMAPAVDGVNLSLRPGDAL
                     GLAGESGCGKSTLALACLGLLPRNATVEGSIRFQGTEVIGAKPGRLRALRWAGISMVF
                     QGAMNAFNPVKRVGDQVVEAILLHESVSEGEARRRMEELFEHVGIASRRANDYPHEFS
                     GGMRQRVMIAMALSCNPPLVIADEPTTALDVMIQAQILNLLARLREERNIALVVITHD
                     LSTLAQVTDRVAIMYAGRIVEEGSASELLTTPRHPYSKALVGAFPVVGDPASRGIPAG
                     LPGDPPDPAARPPGCAFHPRCPSAFEPCDHVDPPLEMHGSRLAACHLVAREEA"
BASE COUNT          337 a          850 c          770 g          425 t
ORIGIN      
        1 gcgttcatgt ccaccccggg gctggctgag gcctcggccg gcgcgcagct cgtggatacc
       61 ctcgagcacc tcttcttgcc cgcgctcacc ttcacgctgg gcctgatcgg ggagtacgct
      121 ctgatcatgc gctccagcgt catcgaggcg atgaacgagg actacgtcac gaccgctagg
      181 gccaaggggt tgcgggaaac gctcgtcctg cgcaagcacg tcgtcccgaa cgcgctcctg
      241 ccgaccgtga cgctcgccgc cctgaacctc gggttcatcg tctcgggagc catcacggtg
      301 gagaccgtgt tctcgtggcc gggactgggc cagctcacct tccgcgccat aaatgggggt
      361 ccggactacc cgctcttgca ggggctcttc ctgctgttct cggtggcggt catcttcgcc
      421 aacctcgcgg cggacctcct gctgggggtg ctcgacccaa ggatcggtcg cgcatgagcg
      481 caggggcagc gcccagcgcc cgcgcggtcc ggcgcacgcg ccgggtcagg gcgctcaagt
      541 cgtcggcaag gctgttcgcc aagaacagga tgggcgtcgc ggggctcgcg atcctcacgg
      601 tgttcgtcgt gctggccctc tttgccgacg tgctggtccc cgaggcgcgc ctctcggtga
      661 cggaggcgcc cggggctcca ctcgagccgc cctcgtcgca gttctggctc ggaaccgacg
      721 agctcgggcg ctcggtgctc gacctcgtca tccaggggtc ccagatctcc ctgctcgtcg
      781 ggtttttggc caccgcgatc tccatggtca tcggctcgct cgtggggatc tccgccggct
      841 acttcggggg cgggatcggg gccctgctca tgcgcttcac cgactggttc ctggtcatcc
      901 cgtttctgcc tcttgctatc gtgctcggtg ctatcctggg ccgcagcctc ttcgtcatcg
      961 cgctcgtcat cgggctcgtc acctgggcgg ggacggcgcg cgtggtgcgt gctcaggtgc
     1021 tggccctcaa ggaacgcccc tacgtcgagc gcgccagggc cctcggagcc agcaaccgcc
     1081 agatcatcgc ccgccacatc ctccccaacg tcttccccct catcttcgcc aataccatcc
     1141 tcgtcgtggc agtcgccatc ctgaccgaga ccacgctctc cttcctgggc ctgggcgacc
     1201 ccttcagcgt ctcctggggc agcatcctcg agtctgcata cgcggaaggc gcgatctcgc
     1261 tcggcgcctg gtggtacctg gtcccgcctg gtgtctgcgt cgtgctggtg gtgctcggct
     1321 tcacgatgtg cggctacgcc ttcgacgaga tcctggaccc gaggctgcgc gatagatgag
     1381 cgagacactc actcccacag gaaccttgct cgagatcaag gacctccgcg tcctgtatga
     1441 ggtgcggggc gtgatggccc ctgcggtgga cggcgtgaac ctctcgctcc ggccgggaga
     1501 cgcgctggga ctagcgggtg agtcgggctg cggcaagagc acgctcgcgc tggcgtgcct
     1561 cggcttgctg ccgcgcaacg cgacggtgga gggctcgatc cgcttccagg gcacggaggt
     1621 tatcggcgcc aagccgggtc gtctgcgcgc gctgcgctgg gcgggtatct cgatggtctt
     1681 ccagggggcg atgaacgcgt tcaaccctgt gaagcgcgtc ggggaccagg tagtagaggc
     1741 gatcctgctc cacgagagcg tctccgaggg cgaggctcgc cgcaggatgg aggagctctt
     1801 cgagcacgtc ggcatcgcgt cgcgcagggc gaacgactac ccgcatgagt tctccggggg
     1861 gatgcgccag cgggtcatga tcgcgatggc gctctcgtgc aacccgccgc tggtgatcgc
     1921 cgacgagcca accacggcgc ttgacgtcat gatccaggcc cagatcctca acctcctggc
     1981 acgcctgcgc gaggagcgaa acatcgcact ggtagtcatc acccacgacc tctcgaccct
     2041 cgcccaggta accgacaggg tggcaataat gtatgccgga cgcatcgtcg aggaaggttc
     2101 cgcctccgag ctcctcacca caccccgcca cccatactcg aaagcactgg tcggcgcctt
     2161 ccccgtggtc ggagacccgg cttcccgggg catccccgcc ggcctgcccg gcgacccgcc
     2221 cgaccccgca gcacgccccc ccggctgcgc cttccatccc cgctgcccca gcgcattcga
     2281 gccttgcgac cacgtggacc cgcccctgga gatgcatggc tcacgcctcg ccgcctgcca
     2341 cctcgtcgcc agggaggaag catgacggag gcaagcggta aa
//
LOCUS       DASTUV010000213         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_989591, whole genome shotgun sequence.
ACCESSION   DASTUV010000213 DASTUV010000000
VERSION     DASTUV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989591"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFT03_04275"
     CDS             <1..290
                     /locus_tag="VFT03_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847391.1"
                     /translation="LSERRTRERDEHRHVGMVVDDRRAVRRALREMDVEILPGRGLDF
                     LDPWGNRWQVLDYSEIQFTKAPNVLSGMGLGSLGKSESALEELRKKGLAPD"
     gene            442..2394
                     /locus_tag="VFT03_04280"
     CDS             442..2394
                     /locus_tag="VFT03_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4847392.1"
                     /translation="MGIAGDIALILVAALLGGLVARRLGLPLILGYIVAGVAVGPNTG
                     GPTVSSVHDIELLAEIGVALLLFTIGLHFSLDELVPVRRVALFGTAAQMALTVAFGYG
                     LGRLLGFGWQEAAWFGALLSLSSTAVVLKSLSEQGVMGTLSSRVIIGMLVAQDLAVVP
                     LIIVLPELGSLGQGLSELGVAALRATAFVAVMVVFGRRVLPWLMARVAAWNSRELFLI
                     SVVAIGLGVGYATYLFGLSFAFGAFVAGLVLSQSDYSHQALADVEPLRDVFAMLFFVS
                     VGMLLDPAFLIQNAGTVALVVALVFAVKGLVFAGVVRAFGYGNIIPFAVGLGLFQVGE
                     FSFVIARVGVEEGAISESSYSIVLSTAVITMSLTPFAMRLAPLLYGRWRERFPKEAMS
                     TFNLPERGLRDHVVVAGYGRVGSFVTRLLARLDQPFVVVDPNPARADEARDSSYPVVY
                     GDAGAEPVLEAAGVRRARLVIVTVPDPVGARLVVERVRSINPNVHVVARTTTVEQLEE
                     LGRLGIYEAVHPESEAGLELGRQALSHLDVPAGDIQRFADEVRRELYAPITRQGMEGD
                     LLAQLERASRQIETEWVRLPENSSLEGRTIGDLGVRTQTGASIVAIVRDDLVLAGPGP
                     DARFEPGDLVGVLGTRDQRAAFVALT"
     gene            2496..>2741
                     /locus_tag="VFT03_04285"
     CDS             2496..>2741
                     /locus_tag="VFT03_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847393.1"
                     /translation="MSAKLGDLAAAVGKGLFAGAAGTVAMTASSTIEMKLRGRAPSSA
                     PAQAAVKVLGVRPVDEKAEARFSNIVHWGYGTAWGGFR"
BASE COUNT          393 a          851 c         1005 g          492 t
ORIGIN      
        1 ccctctcgga gaggcggacg cgggaacgcg acgaacatcg ccacgtcggg atggtggttg
       61 acgacaggcg ggctgtgagg cgggcgctca gagagatgga cgtggagata ctgccaggta
      121 ggggcctgga tttcctcgac ccgtggggca accgctggca ggtcttggat tactccgaga
      181 tccagttcac caaagctcca aacgttttga gcggcatggg gctcggcagc ctggggaaga
      241 gcgagagcgc cctggaggaa ctgcggaaga agggcctggc tccggactag ggggctctct
      301 atacgggcgg ggtgaggtcc gagatagagg agatcgtcgg ccgcgagacg agggcctgga
      361 ataccgagga cgtggacctc ctgctactcg agtaccggta ggagccgcga gcgatgcccg
      421 ggtgaggtag tgtagaggcg cgtgggcatc gcaggggaca tagcgctcat actcgtggct
      481 gccctcctcg gcgggctcgt cgcccggcgg ctcgggctgc ccctgatcct tggctacatc
      541 gtggccggcg tcgctgtcgg gcccaacacg ggtggtccga ccgtcagctc ggtccacgac
      601 atcgagctgc tcgcggagat cggggtggcg ctgctcctgt tcaccatcgg gctgcacttc
      661 tcgctggacg agctcgtccc tgtgcgccgc gtcgctctct tcgggacagc ggcccagatg
      721 gcgctcaccg tagccttcgg ctacgggttg gggcggctgc tcggcttcgg gtggcaggag
      781 gcggcgtggt tcggggcgct gctctcgctc tccagcacag ccgtagtcct caagtcgctc
      841 tcggagcaag gcgtgatggg gaccctgtcc agccgggtca tcatcgggat gctcgtcgcg
      901 caggatctgg ctgttgtgcc cctcataata gtgctgccag agctcgggag tctgggccag
      961 ggtctctccg agctcggcgt agcggcgctg cgggctaccg ccttcgtcgc ggtgatggtg
     1021 gtgttcggga ggcgggttct gccgtggcta atggcccgtg tggccgcctg gaactcgcgc
     1081 gagctgttcc tgatctccgt cgtcgccata gggctcgggg tgggttatgc gacctatctc
     1141 ttcggcctct cgttcgcctt cggcgctttc gtggccgggc tcgtgctcag ccagtcggac
     1201 tacagccacc aggcgctcgc cgacgtggag cccttgaggg acgtcttcgc gatgcttttc
     1261 ttcgtttcgg tagggatgct cctcgacccg gctttcctca tccagaacgc ggggaccgtg
     1321 gcgctggtgg tcgcgctcgt cttcgcggtc aaggggctcg tgttcgctgg cgtggtgcgg
     1381 gcgttcgggt acgggaacat catcccgttc gcggtggggc tcgggctctt ccaggtcggg
     1441 gagttctcgt tcgtgatcgc gcgcgtcggc gtggaggagg gggccatctc cgagagcagt
     1501 tactccatcg tgctgagcac ggcggtgatc acgatgtccc tgaccccatt cgccatgcgc
     1561 ctggcgccgc tactctacgg ccgctggcgc gagcggttcc caaaggaagc gatgagcact
     1621 ttcaacctgc ccgagcgggg ccttcgcgac cacgtggtcg tcgcgggcta cggcagggtc
     1681 ggctccttcg tgacccggct tctcgcgcgc ctggatcagc cgttcgtcgt cgtagatccg
     1741 aaccccgctc gggccgacga agcgagggac tcaagctacc ccgtagtcta cggcgacgcg
     1801 ggagccgagc cggtgctcga ggccgccggg gtgaggcggg cgcggctggt gatcgtcact
     1861 gtgcctgacc ccgtgggggc gcgcctggtt gtcgagcgcg tacgctccat caacccgaac
     1921 gtgcacgtcg tcgcccgcac cacgacggtg gagcaactgg aggagttggg gcggctcgga
     1981 atctacgagg ccgtgcaccc cgagtcggag gcggggctgg agctcgggcg ccaggccctc
     2041 tcgcacctcg acgtgccggc cggggacatc caacggttcg ccgacgaggt gcgccgcgag
     2101 ctgtacgccc ctatcacccg tcaggggatg gagggcgacc tactcgcgca actcgagcgg
     2161 gcctcgcggc agatcgagac cgagtgggtc aggctgcccg agaacagctc gctagagggg
     2221 cgcacgatcg gggatctcgg ggtgcgcacc cagaccggcg cctcgatagt ggcgatagtg
     2281 cgcgacgacc tggtgctcgc cggacccgga cccgacgccc gcttcgaacc cggcgacctg
     2341 gtaggggttc tcggtacccg ggatcagcgc gccgccttcg tggcgctgac ctagcatggc
     2401 cacgcgccag cgttacctgt ccgccggttc agggtaacta ccggacgtcc ggcagacggg
     2461 tcgagatatc tctcccggat cagaaggaga tcattatgag tgcgaaactc ggagacttgg
     2521 cggctgcggt gggcaagggg ttgttcgccg gcgccgccgg gaccgttgcc atgacggcgt
     2581 cgagcacgat cgagatgaag ctgcgcggcc gggccccgag ctcggcccct gcgcaggcgg
     2641 cagtgaaggt actcggcgtc cggcccgtag acgagaaggc cgaggcgcgt ttttccaaca
     2701 tcgttcactg ggggtacggg acggcgtggg gaggcttccg g
//
LOCUS       DASTUV010000214         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_539802, whole genome shotgun sequence.
ACCESSION   DASTUV010000214 DASTUV010000000
VERSION     DASTUV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539802"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFT03_04290"
     CDS             complement(<1..717)
                     /locus_tag="VFT03_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU4847394.1"
                     /translation="MERDRLIRTMQSILGPEGVISEREQLRSYECDGLMNYRVIPDLV
                     VLPESAEQVQRIVRTCYEEGIPFVARGSGTGLSGGALPVEAGILIVMSRMRKILEVDI
                     PNQRVVVEPGVINVWVSQEVADEGYYYAPDPASQIVSSIGGNMAENSGGVHCLKYGFT
                     TNHVMGAEVVLPDGSVAHIGGGKAPDAPGYDLIGAFVGSEGTLGIATRITLRIVRKPE
                     SVRTLLAAFKDTEEAGGAVSG"
     gene            1036..2253
                     /locus_tag="VFT03_04295"
     CDS             1036..2253
                     /locus_tag="VFT03_04295"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEU4847395.1"
                     /translation="MPVTVRDVLQMECLSQARVVAGERGLDREVYWITVGEEPDLPEW
                     VFGGELILSTLFAVEPERRDEYVKGLSDRGVAGLMLKPERFLGTIPQSVLETADRESF
                     PVAEVPIGVLWSRVLAGFYRHLLAEQTEFIRVETEMRLRGGFFDELLSGQISGEEILR
                     RAALLGCDLAGDAVMLVFDLADMDGVARKLDDLQVQYLKTLLYESVNGAVQEVHRNCI
                     CIPRPDGVLLLLGSPVDDREMVAKHILRRCRERLKNMPVHAGLGEPCESPEQITRSCR
                     EADSALRVGQRLQSARDVEGRIHSFSALGVQRILFALSQDSPETLRGFEESTVGPVMD
                     YDREHGTQLLETLSTYMKCDGHILEVAALLHVHKHTVRYRLRRVTELTGLDVTKFDDA
                     AQLYLAVRTTELR"
BASE COUNT          453 a          780 c          767 g          454 t
ORIGIN      
        1 ccccgagacg gcgccgcccg cctcctcggt gtccttgaac gccgcaagca gcgtcctcac
       61 cgactcgggc ttgcgcacta tacggagggt aatcctggtc gcaatcccca gcgttccttc
      121 cgagcccacg aacgccccga tcaggtcgta cccaggcgcg tcgggagcct tgccgcctcc
      181 gatatgcgcc accgagccgt cgggcaatac gacctcagcc cccatgacgt ggttggtagt
      241 gaagccgtat ttcaaacaat gcacgccgcc gctattctcc gccatgttcc cgccgatcga
      301 ggagacgatc tggctcgccg ggtccggcgc gtagtagtaa ccttcgtcgg cgacctcctg
      361 agagacccac acgttgatca cgccgggctc gacgaccacc cgctggttgg ggatgtctac
      421 ctcgaggatc ttgcgcatcc tcgacattac tatgagtatc cccgcctcca cgggcaaggc
      481 cccgccggag agaccggtcc ccgaacccct cgccacgaac gggatgcctt cctcgtagca
      541 ggtcctgacg atccgctgca cctgctccgc actctcgggc aagacgacga ggtcggggat
      601 gaccctgtag ttcatcaggc cgtcgcactc gtaagatcgg agctgctcgc gctcggagat
      661 gacgccctcc ggcccgagaa tagactgcat cgtccttatg agcctgtccc gctccatgtg
      721 cgcctcctcc cggagaccac ctctcgccag aaccttcgtg cgaaggtcaa gttggtcgag
      781 tgttctccgt cgcccttccc cttcgatgtc tttatcacct ggtcaacgtc cactgcggac
      841 gctgtctccg aatcctccgc aattctatcc caaagccggg ctcgaagttg cgtagcgaga
      901 actcgttgcc gttcgcaccg tttatggtgg gtaccggctt cgcagctttc aatggcacgg
      961 tgagcttcat gctatgatcg tttctaaagg tcggagccgg tccagagatt ggcctggtgg
     1021 ggtaaggaaa acagcatgcc ggtcacagtc agggatgtat tgcagatgga gtgcttgagc
     1081 caggcgcggg tcgtggccgg ggagcggggc cttgaccgcg aggtgtactg gatcactgtt
     1141 ggagaggagc cggatctccc ggagtgggtc ttcggggggg agttgatcct gagcacgctc
     1201 ttcgccgtgg agcccgagcg gcgcgacgag tacgtcaaag gcctctcgga ccggggagtg
     1261 gcggggctga tgctcaagcc cgagaggttt ctcggcacca taccccagag cgtgctcgag
     1321 accgcagacc gggagtcctt tccggtggcc gaggtcccga tcggcgtcct ctggtcccgc
     1381 gtgctggccg gcttctacag gcacctcctc gccgagcaga ccgagttcat cagggtggag
     1441 accgagatgc gcctgcgcgg gggcttcttc gacgagctcc tctctggtca gatatcgggg
     1501 gaggagatcc tccgccgggc ggcgctcctt gggtgcgacc tcgccggcga cgcggtgatg
     1561 ctcgtcttcg acctggctga catggacggc gtagccagga agctcgacga cctgcaggta
     1621 cagtatctga agaccttgct ctacgaaagc gtcaacggag cggtgcagga ggtccatcgg
     1681 aactgcatct gcatcccgcg cccggacggc gtgttgctgc tgctcggaag cccggtcgac
     1741 gaccgcgaga tggtcgcgaa gcacatcctg aggcggtgcc gggaacgcct gaaaaacatg
     1801 cctgtgcacg cggggttggg tgaaccatgc gagagccccg agcagataac ccgttcgtgc
     1861 cgggaggcgg attccgcctt gcgcgtcggg caacgcctgc aaagcgcaag agacgtagag
     1921 ggcaggatcc actccttctc ggccctgggc gtgcagagga tacttttcgc gctcagccag
     1981 gacagccccg aaacccttcg aggcttcgaa gagagcaccg tggggccggt catggactac
     2041 gacagagaac acggtacgca actactagag accctgagca cctacatgaa gtgtgacggc
     2101 cacatcctgg aggtcgccgc ccttctccac gtccacaagc acaccgtccg ctacaggctc
     2161 cgcagggtga cggagctcac cgggctcgac gtgacgaagt tcgatgacgc ggcccagctc
     2221 tacctggccg tcaggacgac cgagttgcgc tgaagcgcct atttgtccag gagaacaaac
     2281 ttggcgggag cttctttagt ctcgcgtaca tagactccgg gtccgcctcg tgactagtat
     2341 ttctgctaaa gggtgtgtcg gcaaagggga aagggatatg gaagcgagca acgcgcctac
     2401 gggatacagc gaccgtctct acaacgagga cctcgctccg gtcacggagc gcac
//
LOCUS       DASTUV010000215         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_685406, whole genome shotgun sequence.
ACCESSION   DASTUV010000215 DASTUV010000000
VERSION     DASTUV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685406"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            881..1450
                     /locus_tag="VFT03_04300"
     CDS             881..1450
                     /locus_tag="VFT03_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU4847396.1"
                     /translation="MELLRVGVLEEDEVYRRGLIAVLNEDDCICIVLGGDRETSPEAA
                     DAEDRLDVAVVCSRLLERVRLSCPLVCLAIPELQGAESCHSSPNVKATLRHDGLTAEE
                     LVASVRAAAAGLQVESPGLSRVNGGVMDSRRIEILKLLAGGADTRTIAADLQIAERTV
                     KTLVHDIQLTLGVSTRAQAVAQAIRLGLL"
     gene            1601..2215
                     /locus_tag="VFT03_04305"
     CDS             1601..2215
                     /locus_tag="VFT03_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847397.1"
                     /translation="MAKTGSQAAFSTDSSDQEVGLEGAGERADLGRRRKRMRTSSKAA
                     VVAVVALATLLVGASSALADSAKFDRRNTFVVNGVQDDGSLMVHFRETGLGNTGDPVE
                     YRLSADANAVYACQNNGGNFPSDPKKTAVSAAVSTDAEFSPDNGNVEADLSIFPPPNT
                     ALTCPGGQHPVLVSITYTNVKITDLDHGVSFNFAGTFSRTFFTI"
     gene            2278..2478
                     /locus_tag="VFT03_04310"
     CDS             2278..2478
                     /locus_tag="VFT03_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847398.1"
                     /translation="MSVHEASMRREASARLLSANASPMPGPPIGSYWGNPKTRTFAEL
                     LIDCEEDRVLRAVLVGMLREAN"
BASE COUNT          491 a          843 c          883 g          496 t
ORIGIN      
        1 ggcgatcccg ttgaggccgc tggcgcgctc accggctgag ccgtgtccgg tcgcgtccta
       61 ccttgccatg ggcagcagcc gcaggtccta tccctggagg tgcgggccgg acccctatcc
      121 gtcggtcgtg tgcctcccta ccgcgaccac gtcagaagtg cttgagacgt ttgggcgttg
      181 gcctctccta acccgcaaga ctatcggggc gtggcagttc cccgaacacc tggtacgcga
      241 gggagacagg tccgttccac gcgtagtgga tatcagggat gtccacgggg gtatcctcga
      301 cttgcctccg cccggcgcaa caggaagtgt ggtcggcgcc cactccttca cttcaataga
      361 ctgtggccgc cttctcgatt ctcggatgat gttcctatcc gtagacccgg gtacgactcg
      421 acgcctcttg cgccggggcg cgaggccacc ttcacccgac atgttggctg tccccctcaa
      481 gcgtggacca gcgcgccgtt cttcctgact gcgctacccc accgtcgcta tcggcgcact
      541 ccgctccaag agacgtacag agggtcagtc ggggaaagat gctcttccca ccaccggtac
      601 ccatgacctg ggaagatgca tcacctgcta acgcggttgc taaccgcagt gggtgagtgt
      661 cacccctccg cgtcggtgag cccctcaggt tgtcgtgtcc ccggggcgtt cgcccggttc
      721 gtgactatga agaaatcaca tcacgatgac ctgggaccgc tgcaagcccg gctgcgcgtg
      781 caccgactgc gttcttgata tgtgacaaga atcacatcgt acgatcgccc tacgggggct
      841 agcgtaggtg ccgctatcca ctgcaatgga gggatgcgag atggaactcc ttcgcgtggg
      901 ggtcctcgag gaagacgagg tttaccgtcg cggcctgata gcggtcctca acgaagacga
      961 ctgtatatgc atcgttctcg gaggtgatcg agagacgtcc ccggaagcgg ccgacgcgga
     1021 agacaggctc gacgtggcgg tcgtgtgttc gaggttgctc gaacgggtgc ggctgtcgtg
     1081 cccactcgtc tgcctggcca tcccggaact ccaaggcgcg gagagctgtc actcgtcgcc
     1141 gaacgtgaag gcgacgctgc gccacgacgg tctgacggcc gaggagctcg tcgcgagcgt
     1201 tcgcgccgct gcagccgggc tgcaggtcga gtcgccgggc ctgagccggg tgaacggtgg
     1261 cgtcatggat tcccgcagga ttgagattct gaagctcctg gcagggggcg ctgatacgcg
     1321 gacgatcgcc gcggacctcc aaatcgccga gcggaccgtc aagacgctgg tccacgacat
     1381 ccagctgacc ttgggagtga gcacgcgggc acaggccgtc gctcaggcca tccgtctagg
     1441 actcctctga ggcacactgg gcgcccaaag gggctgcccg gtcgcccaac ggggcagtat
     1501 caggtctcca ataggtactt ttgtgcgttc gttcatgtcg cccgagccat cggaaggggc
     1561 ccgaaggtcc gtcgtgatgc tggtgtgagg agatcgagca gtggctaaga ctggaagcca
     1621 agccgctttc tcgacggact cgagcgatca ggaggtgggg ttagaaggcg ccggggagcg
     1681 ggcggatcta ggaagaagga ggaagcgcat gaggacatcc tcgaaggccg cagtcgttgc
     1741 ggtcgttgcg ctcgccacgc tgcttgtcgg ggcgagctcg gcactggccg acagcgcgaa
     1801 gttcgacagg aggaacacgt tcgtcgtcaa cggagtccaa gatgacggct cgctgatggt
     1861 gcacttcagg gagacgggtc tgggcaatac gggcgacccc gtcgagtacc ggctctcggc
     1921 cgacgccaat gccgtatacg cgtgtcagaa caatgggggc aacttcccca gtgacccaaa
     1981 gaagactgct gtatcggcgg cggtctcaac cgacgcggag ttcagtcccg ataacggcaa
     2041 cgtcgaggca gacttgtcga tcttcccgcc accgaacaca gcgctaacgt gtcccggcgg
     2101 ccagcacccg gtcctggtca gcatcacgta cacgaacgtc aagatcactg acctcgacca
     2161 cggtgtgtct ttcaacttcg cgggaacgtt ctcacggacg ttcttcacga tctagccact
     2221 cagcaaggcc aaatacccag gagccggggc aacccggctc ctgggttatg gacgagagtg
     2281 agcgtccacg aggcttccat gaggcgggag gctagcgcca ggcttctcag cgccaacgct
     2341 tcgcctatgc ctggacctcc catcggctcc tattggggga acccgaagac ccgcaccttc
     2401 gccgagctcc tgatcgactg cgaggaggat cgggtcctcc gggctgtgct cgtggggatg
     2461 ctgcgggaag cgaactgagt cctttcggcc gctgggcgca gcggaagccc tcaccgcaca
     2521 gcgagccagc ggtgatttga acccgccact cagttttggg ctggcaggga gcgggtacta
     2581 caggggtctc ggcaagaagg gagacgcccg cctcgcccct cgcaggtggg cgtcttccgt
     2641 agggagtgcg ccatgcggat acttgaaccg tttacgtcca acaccaggct cgtcgcgttg
     2701 tgctctcggt gtc
//
LOCUS       DASTUV010000216         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_6539, whole genome shotgun sequence.
ACCESSION   DASTUV010000216 DASTUV010000000
VERSION     DASTUV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6539"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFT03_04315"
     CDS             <1..318
                     /locus_tag="VFT03_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847399.1"
                     /translation="AVLMGSQGTAIYGQSAKDPATSVLSVMRNLTTKPPKITAIRSDI
                     EGIPEERVYEALDAVSASFRSGKGAADPYKNFVWNLKRPNRTRRNGGDASRILSGLDR
                     MSD"
     gene            373..840
                     /locus_tag="VFT03_04320"
     CDS             373..840
                     /locus_tag="VFT03_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU4847400.1"
                     /translation="MDDNRVEAYWQGYLETLPADSPVRDEQYIAEGWGDSPEMADELG
                     ALILAGTKTATCSALWEYEAEGESLPETGLKTIVLDGNGDPMCIVETTEVEVWPYDEV
                     DARFAREEGEGDRSLEYWREAHWRFFSRTLPNIGKEPATDMPLVCERFRVVYR"
     gene            982..2121
                     /locus_tag="VFT03_04325"
     CDS             982..2121
                     /locus_tag="VFT03_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4847401.1"
                     /translation="MSDTHLGYTRYAKLHPETGRNQREVDVQEAYARAVDAILEREVD
                     LVIHSGDVFDSVRPATHVIIGFLKQTARITARADIPYLVAAGNHETPRLRTTTAALEY
                     ANLVNAYSAHGFEIDYHPIEVGDVTVGVTLVPHGAVATAGAVTPEHDADINILVTHGL
                     VPNLEARQHEMGEANLHSGMLEGAFDYIALGHYHDFHEHKENSYYSGATERFSFGEVA
                     SEPGFAIVEFDGGEVSVEHVPIETRPMIDLPFIRARNLDAQELTEEVRRQTENVDLDG
                     AIVRLRAYDVRRGVASGVDREILRDLQRRCLNFSLEIHAEESPDTTNGGPATAVFGPL
                     QEEFAAFVKGRRESGDLEKAFAEEFLEKGRGYLAQAASEEPGGVA"
     gene            2118..4976
                     /locus_tag="VFT03_04330"
     CDS             2118..4976
                     /locus_tag="VFT03_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEU4847402.1"
                     /translation="MNLERLHIENYKQLRDPVELFPPEGAIGVVGPNGAGKSTLFESI
                     LWALFGSKGAGPRFANESIPWSGGSTKDPTVVEVTLTTGNGPYTVRRQLKSNATTAEA
                     RDPGGKTVVTGAADVTRWVEEDLLKMDRTTFEATFYAKQKELRFFAHDDGISRVRRIS
                     KMLGISGVEVAQQFLRADRNELRSEARVIEARLAEADLESLQSELEEAKAEIRRLEAD
                     LEKVLTEYEEAEGELKAARETREKLDATYREHTRLTGELREAEGEKRRAEDRTAEAGK
                     DLEELAKAREELGRLGPELEKLPELEEELEKLEEDRRRAERRDAARRELRQAQSRVAA
                     IESEVWDKLEGLDGDEPLPGWSALFELDGPELLEEAVAVLEGAVGELALAEERYEALR
                     ELAASHEDFELVEKELREAEGRHEEAVVEVRKILEELEDVSDGEDLEKWERELREEEE
                     KLREVSATHRGRAAAHEREAKNVDRAREAIDSGADDHCPTCHRGFEEEEQVEISDTLR
                     RQAAALRRLAARENEEAEKLSEAAAATEKKLQGVSAKLARWREVREALARAEDRAAER
                     LEALERAREHRRELGARLESSVAPTQEDLAQARARYEELRSLRDARPGVVSLAREHAD
                     KTRRAAEISEELEDLAGVSYDPEDHQEKRQEKARLDESRGRAAELERRLASRPEVETA
                     LESARRLAGEADKKAGDLRREISALGFDEAAYVAVTERVAAVEERAGRLRDKREHLGG
                     EWKDADHRIERANTELGRLDADRKLANTRASEAARMDEMDALFTLFFKSLTARARPML
                     EAEASALVRELTDNRYERMEFDENYRVRLLDRFDDSYAIDRFSGGEADVASLSARVAL
                     SKIIASRGGATLGFLILDEVFGSLDADRRNNVLLALERLKRFFGQIFIISHVAEVQES
                     ALVDEVWMLEEDEDGKSTVHRVDQSSTVPVELLQGQEQ"
     gene            complement(5100..>5526)
                     /locus_tag="VFT03_04335"
     CDS             complement(5100..>5526)
                     /locus_tag="VFT03_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU4847403.1"
                     /translation="ALPKRALRRYVFRSTLPFLVLISAAALLSSLVFDLAAWVFAALL
                     LLLPAALFGWLRYRDAGWTLEEDRLVVRSRLLGRTTAIAPRRRLQSRTTLRSPFQRRV
                     SLATFEAQVASGGGGAELRVTDLGADAADALIENLGPGA"
BASE COUNT         1108 a         1702 c         1988 g          728 t
ORIGIN      
        1 gctgtcctaa tgggctcgca gggcaccgcc atctacggcc agagcgccaa ggaccctgcg
       61 acctccgtcc tctccgtcat gcgcaacctc accaccaagc cccccaagat aaccgccatc
      121 cgctccgaca tagaaggcat ccctgaggag cgcgtctacg aggctctcga cgccgtgagc
      181 gcttccttcc gctccggcaa gggcgccgcc gacccgtaca agaacttcgt ctggaacctc
      241 aagcgtccca accgaacccg ccgcaacggc ggagacgcct cgcgcatcct ctccgggctc
      301 gacaggatga gcgattagcc ccggccgggg tttcgtacgt gtcgcgcacc cgatttcgct
      361 tcggagccac tagtggacga caaccgggtt gaggcatatt ggcagggcta cctggagacg
      421 ctaccagcag attcgccggt gcgcgatgaa cagtacatcg cggaaggttg gggcgactct
      481 cccgagatgg cggacgagct cggagcactg atcttggccg ggaccaagac ggcgacctgc
      541 tcggcgctct gggagtacga agccgagggt gagtctttac ccgagacagg gttgaagacc
      601 atcgtccttg acgggaacgg cgatcctatg tgcatagtcg agacgaccga ggtagaggta
      661 tggccttacg acgaggtcga cgcccggttc gcccgcgaag agggagaggg agatcgctcg
      721 ctggaatact ggcgggaggc gcactggcgc ttcttctcgc gcacgttgcc gaatataggc
      781 aaggaaccgg caacagacat gccgttggtg tgcgagcgat ttcgtgtggt ttacagatag
      841 aacaaggtta tgcgggagag ctccgaagcg gcgcaagagc gaggtccgac ggggagaggt
      901 tagaggaaaa atggcacact ccagctagaa tagctctggc ttagaagaaa gaaggtcgtc
      961 agcatagtac gcatagccca catctcggat acgcacctag ggtacacaag gtacgcgaag
     1021 ctgcaccccg agacggggcg aaaccagcgg gaggttgacg tacaggaggc gtacgcgagg
     1081 gcggttgacg ctatcctgga gcgggaagtg gacctggtga tccactccgg cgacgtattc
     1141 gactctgtga ggccggcgac gcacgtcatt atcggcttcc tgaagcaaac cgcccgcatc
     1201 accgcccgcg cggacatccc atacctggta gccgcgggca accacgagac gccgcgcctg
     1261 cgcaccacca cagccgcgct cgagtacgcg aacctggtca acgcctactc ggcccacggg
     1321 ttcgagatcg actaccatcc tatagaggtc ggagacgtaa cggtcggcgt gaccctggtg
     1381 ccgcacgggg cggtcgccac cgcgggcgcc gtcacgcctg agcatgacgc ggacatcaac
     1441 atcctggtca cgcacgggct ggtgccgaac ctcgaggcga ggcagcacga gatgggcgag
     1501 gcgaacctgc actcggggat gctggagggc gccttcgact acatcgcgct cggccactac
     1561 cacgacttcc acgagcacaa ggagaactcc tactactcgg gtgcgacaga gcggttcagc
     1621 ttcggcgagg tcgcgtcaga gccggggttc gctatagtcg agttcgacgg cggcgaggtc
     1681 tcggtcgagc acgtgccgat agagacgcgt ccgatgatag atctcccgtt catccgggcc
     1741 cggaacctcg acgcgcagga gctgacggag gaggtccgga ggcagaccga gaacgtcgac
     1801 ctggacggcg cgatcgttcg ccttcgcgcc tacgacgtga ggcgcggggt ggcgagcggg
     1861 gtggacaggg agattctccg ggacctgcaa cgccgctgcc tgaacttcag cctggagatc
     1921 cacgccgaag agagccccga cactacgaac ggcggccccg cgacggcggt cttcggcccc
     1981 ttgcaagagg agttcgcggc gttcgtgaag gggcgaaggg agagcgggga cctcgaaaag
     2041 gcattcgcag aggagttcct ggagaagggg cgcgggtacc tggcgcaggc cgcgagcgag
     2101 gaaccggggg gcgtggcttg aacctggagc ggctccacat cgagaactac aagcagctcc
     2161 gggacccggt cgagctcttc ccgccggagg gcgccatcgg ggtcgttgga cccaacggtg
     2221 cgggcaagtc caccctcttc gagtcgatcc tgtgggctct cttcggctcc aagggcgccg
     2281 gcccccgctt cgccaacgag tccatcccct ggagcggcgg ctcgacgaag gacccgacag
     2341 ttgtcgaggt taccttgacg accggcaatg gcccctacac cgtcaggcgt cagctcaaga
     2401 gcaacgccac gaccgccgag gcgcgcgacc ctggcggcaa gaccgtcgtg acgggcgcag
     2461 cagacgtcac ccgctgggtc gaggaagacc tgttgaagat ggaccgcacg acgttcgagg
     2521 ccaccttcta cgccaagcag aaggagctaa ggttcttcgc gcacgacgac ggtatcagcc
     2581 gcgtgcgccg catcagcaag atgctcggca tcagcggcgt cgaggtcgcc cagcagttcc
     2641 tgcgcgccga ccgcaacgag ctgcgctccg aggcccgcgt catcgaagcc cgcctcgccg
     2701 aggccgacct ggaatccctc cagagcgagc tcgaggaggc gaaggcggag atccggcgcc
     2761 tcgaagctga tctggagaag gtcttgaccg agtacgaaga agcggaaggg gagctgaagg
     2821 ccgcccgcga gactcgcgag aagctagacg ccacctaccg cgagcataca cgacttacag
     2881 gggaactccg ggaggccgag ggcgagaagc gccgggcaga ggacaggacg gcggaggccg
     2941 ggaaggacct cgaagaactc gcgaaagcca gggaggagct cgggcgcctc ggacccgagc
     3001 tggagaagct gccggagctc gaggaggagc tggagaagct cgaggaggac aggcggcggg
     3061 cggagaggcg ggacgcggcg cggcgtgagc tgcggcaggc gcagtccagg gtcgccgcca
     3121 tagagtccga ggtctgggac aagctggagg gtcttgacgg cgacgaaccg ctccccggtt
     3181 ggagcgcgct cttcgagctc gacgggccag agttgctcga agaggccgtc gctgtgctgg
     3241 agggggcggt cggggaactc gcgctggccg aggagcggta cgaagcgctg cgggagctcg
     3301 cggcctcgca cgaggacttc gagctcgtgg agaaggagct tcgcgaggca gagggccggc
     3361 acgaggaggc ggtcgtcgag gtaaggaaga tcctggagga gctagaggac gtctcggacg
     3421 gcgaggacct ggagaagtgg gagcgcgagc ttcgcgagga ggaagagaaa ctgagggagg
     3481 tctccgccac acaccggggc cgggccgccg cccacgagcg cgaggcgaag aacgtggaca
     3541 gggcccgcga ggccatagac agcggggccg acgatcactg ccccacctgc caccgcggct
     3601 tcgaggaaga ggaacaggtt gagatctccg acaccctgcg ccgccaggcc gccgccctca
     3661 ggcgcctggc cgccagggag aacgaggaag cggagaagct ctcggaggcc gcggcggcca
     3721 cagagaagaa gctgcaaggg gtctcggcga agctcgcgcg ctggcgagag gtcagagaag
     3781 ccctggcccg cgccgaggac cgcgccgccg agaggctcga agccctcgag cgagccaggg
     3841 agcacaggcg cgagctgggg gcgcgcctcg agagctccgt agccccgaca caagaggatc
     3901 ttgcgcaggc ccgcgcccgc tacgaggagc tccgctctct gcgcgacgcc cgcccgggcg
     3961 ttgtgagtct ggccagggaa cacgccgaca agaccaggcg cgccgcggag atctcggagg
     4021 agctcgagga cctggcaggc gtctcttacg accccgaaga ccaccaggag aagaggcagg
     4081 agaaggcccg gctggacgag tcgaggggac gagccgcgga gctggagcgc cgtctcgcat
     4141 cccggcctga ggtggagacg gcgctcgaaa gcgcccggcg gctggcgggc gaggcagata
     4201 aaaaggcggg cgacttgcgg cgagagatat ccgccctggg cttcgacgag gcggcgtacg
     4261 tggcggtcac cgagagggtg gcggccgtgg aagagagggc gggcaggctg cgggacaagc
     4321 gcgagcacct tggcggtgag tggaaggacg cggaccacag gatcgagagg gccaatacgg
     4381 agctcgggcg gctggacgcg gatcgcaagc tggccaacac gagggcgtcc gaggcggcca
     4441 ggatggacga gatggatgcg ctcttcaccc tctttttcaa gagcctcacg gcgcgggcgc
     4501 ggcctatgct cgaggccgag gcgtccgctc tggtgcggga gctcacggat aaccgctacg
     4561 agcgcatgga gttcgacgag aactacaggg tgaggctgct cgaccgattc gacgattctt
     4621 acgccataga ccgcttctcg ggcggggaag cagacgtggc gagcctctcg gccagggtcg
     4681 ccctctccaa gatcatcgcc tcccgcggcg gcgcgactct ggggttcctg atcctggacg
     4741 aggtcttcgg gagcctggac gcggaccgcc gcaacaacgt gctcctagcg ctcgagcgcc
     4801 tcaagcgctt tttcggccag atcttcatca tctcccacgt cgccgaggtg caggagtccg
     4861 ccctcgtgga cgaggtctgg atgctcgagg aggacgagga cggcaagagc acagtccacc
     4921 gcgtcgacca gagctcgacc gtacccgtcg agctgctcca gggccaggag cagtaagggc
     4981 gcccttcaac gtcgaggagc aggccctcca ggttcagatt accgacgccg cctgagctac
     5041 gatcgcgaag agagaaccaa accacatggg gctggtgcac tcgccagagc gttactccgt
     5101 caggcccccg gccccagatt ctctatcaag gcgtcggcgg cgtcggcgcc gaggtcggtg
     5161 acgcgcagct ccgctccgcc gccgccagag gctacctgcg cctcgaaggt ggcgagactg
     5221 acgcgccgct ggaaagggct ccggagcgtg gtgcgcgact gcagccggcg ccgaggcgcg
     5281 atagcggtgg tacggcccag gagccgcgag cggacgacga ggcggtcctc ctcgagcgtc
     5341 catccggcgt cccggtagcg aagccagccg aagagggcgg ccggtagaag tagtagcagc
     5401 gccgcgaata cccaggcggc caggtcgaag acgagggaag agaggagcgc ggccgcggag
     5461 atcaggacca gaaaaggcaa ggtggacctg aaaacgtagc gccggagggc tcgcttcggg
     5521 agggcg
//
LOCUS       DASTUV010000217         4949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_614314, whole genome shotgun sequence.
ACCESSION   DASTUV010000217 DASTUV010000000
VERSION     DASTUV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614314"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1302)
                     /locus_tag="VFT03_04340"
     CDS             complement(145..1302)
                     /locus_tag="VFT03_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4847404.1"
                     /translation="MDVERQEIGVGLVGYRFMGRAHSNAYRQVGRFFDLELSPRMRAV
                     CGRDEAGVKGAAALLGWESYETDYRRLLERDDVDLVDVATPGNTHHEIAIAALEAGKH
                     VLCEKPLANTLEEAREMLETSRRAGTVNMVCFNYRRAPAVQLAKQLIEEGRLGEIRHW
                     RAVYLQDFILDPEMPMIWRFQREVAGSGALGDIGAHIIDLAHFLVGPITEVVGATETF
                     IRERPLEAPDGGTGEVTVDDAVAFLARFENGAMGTFEATRLAPGRRNHNSFEINGSKG
                     SVLFDLERMNELSAYFEDDEPHVKGFRTVLVTEPDHPFMGAWWPPGHIIGYEHTFVHT
                     IKDLLDGIAAGESPAPSFEDGYRCQAVLDAVERSVASRSWTQPAGSDATVD"
     gene            complement(1293..2255)
                     /locus_tag="VFT03_04345"
     CDS             complement(1293..2255)
                     /locus_tag="VFT03_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU4847405.1"
                     /translation="MKVGVFTVLLADRPLAEALDYVREAGCEAVEIGTGGYPGDAHCK
                     PRELLGDSGALDEFRKAVESRDLEISALSCHGNPLHPDGEMAREHDELFRDTVRLAAE
                     LGVGSVITFSGCPGDSEGSKRPNWVTCPWPPDFLETLEWQWREKVEPYWQEAASFAGE
                     HGVKVAIELHPGFVAYNTDSFWRLREIGGETIGVNFDPSHLFWQQMDPLVCLRELGDA
                     IFHVHAKDAWVDPQNVRRNGVLDTKPYTDEMNRSWIFRTVGYGHGPEFWRALVSELRL
                     AGYDGVISIEHEDSLMSVNEGFTKAVSFLKDTVITEQPGEAWWT"
     gene            complement(2357..4354)
                     /gene="uvrB"
                     /locus_tag="VFT03_04350"
     CDS             complement(2357..4354)
                     /gene="uvrB"
                     /locus_tag="VFT03_04350"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU4847406.1"
                     /translation="METRSNFKVNSEYRPTGDQPQAIERLASGLDAGMRAQTLLGVTG
                     SGKTHTMARVIEKAGRPALVIAHNKTLAAQLASEFSEFFPNNAVEYFVSYYDYYQPEA
                     YVPRTDTFIEKDAQINEDIDRLRHSTTSSLFTRRDVIVVASVSAIYGLGSPDEYRSKM
                     VLLERGGFYDLDDVLRDLVRIQYTRNDFQLARGTFRVRGDVLEVHPAYQDTIYRISLF
                     GDEVENMTEVDPLTGEILRELDTLTVYPATHFVTDEERLTLAVRNIEAELEERTTELE
                     REGKVLEAYRLRQRTRYDLEMMREVGYCSGIENYSRHLDGRAAGSTPYTLLDYFPDDY
                     LTFVDESHITLPQVRGMYTGDRSRKTTLVEFGFRLPSALDNRPLMWDEFLLKTNQMVF
                     VSATPGPYELENSEQIVEQIIRPTGLVDPEVEVRSTQNQIDDLLNEVAKRTERNERVL
                     ITTLTIKMAEDLTDYLLEHGVKARYMHANIETLDRIQIIRGLRTGEFDVLVGINLLRE
                     GLDLPEVTLVAILDADKEGFLRGERALIQTIGRAARNVNGRVIMYADKETEAMRIAIG
                     ETNRRREIQVAYNEQNDIEPRTIVKGVSDILIAAEAKGLYKADKRSARETPRDPDELQ
                     NLIADLQAEMLEAAEELKFEYAAKLRDEIKDLERQLQEVAS"
     gene            complement(4338..>4949)
                     /locus_tag="VFT03_04355"
     CDS             complement(4338..>4949)
                     /locus_tag="VFT03_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU4847407.1"
                     /translation="YSSKKSRYGRSRARRRLLVLVLLVLAAGIAALLAVLPLIRQAPD
                     AVRRTVYPLRYEETIRDVSAEYGLEPTFVAAVVYTESRFRPDAKSHQGAYGLMQLLPE
                     TARFIQERSGIEGRYRDPKVNLRMGAWYLSYLDGRYMGDERLVLAAYNSGEGRVDAWI
                     SDKGFDVGEDIPFKETHHYVQNVLEAQSVYEELYGRNLYRNSE"
BASE COUNT          803 a         1578 c         1551 g         1017 t
ORIGIN      
        1 ctccaggtgc ctgaggtgtt ggatcccctg ttccatcagg tacgatgttg gatcttcgcg
       61 cgcttcagcc ggtctatccg gtcggatatc tcttcggcag agccaccgga gatgacgtac
      121 gagtagcgaa ggcgtgccac cggtctagtc tacggtggcg tcgctccccg caggctgtgt
      181 ccagctcctg ctcgcgacgc tgcgctccac ggcgtcgagt acggcctggc agcggtagcc
      241 gtcttcgaag ctcggcgcag ggctctctcc agcggctatc ccatccagca agtcctttat
      301 cgtgtgcacg aaggtatgct cgtagccgat gatgtgcccc ggaggccacc aggctcccat
      361 gaacgggtga tcgggctcgg ttacgagtac ggtccgaaaa cctttgacgt gcggctcgtc
      421 gtcttcgaag tacgcggaca gctcgttcat gcgctccagg tcgaagagga cgcttccctt
      481 ggagccgttg atctcgaagg agttgtggtt cctgcggcct ggagcgagcc tcgtggcctc
      541 gaaggtcccc atcgccccgt tctcgaagcg tgccagaaaa gccacggcgt cgtccacggt
      601 gacctctccg gttccgccgt cgggtgcctc gagcggccgc tccctgataa acgtttccgt
      661 ggcgccgacg acctctgtta tcgggccgac gaggaagtga gccagatcga tgatgtgggc
      721 gcctatatcc ccgagggcgc ccgacccggc cacctccctc tggaagcgcc agatcatggg
      781 catctccggg tcgaggatga agtcctgcag gtaaacagcc cgccagtgcc tgatctcgcc
      841 gagccgtccc tcctcgatga gctgcttcgc gagctggacg gcgggcgccc gccggtagtt
      901 gaagcagacc atgttcacgg tgccagccct tcgggaggtt tcgagcatct cacgtgcctc
      961 ctccagggtg tttgccagcg gtttctcgca gaggacgtgc ttgccggcct cgagcgcggc
     1021 gatagcgatc tcgtggtgag tgttccccgg cgtagcgaca tcaacgaggt ctacgtcgtc
     1081 gcgctccagg aggcggcggt agtccgtctc atagctctcc caccccagaa gtgcggcggc
     1141 gcccttcacg cccgcttcgt cgcgcccgca gacggcgcgc atcctcgggg agagctcgag
     1201 gtcgaagaag cgcccgacct ggcggtaggc gttggagtgg gcgcgcccca tgaacctgta
     1261 cccgacgaga ccgaccccga tctcttgacg ctctacgtcc accacgcctc ccctggctgc
     1321 tcggtgatga ccgtgtcttt gaggaagctc accgctttcg taaacccctc gttcacgctc
     1381 atgagggagt cctcgtgctc gatggagatc acgccgtcgt acccggcgag ccgcaattcg
     1441 ctcacgaggg cccgccagaa ctccggtccg tgaccgtagc cgacagtacg gaagatccag
     1501 gagcgattca tttcgtcggt gtacggcttg gtgtcgagaa ccccgtttcg tcttacgttc
     1561 tgcggatcga cccacgcgtc cttggcgtga acgtggaaga ttgcgtctcc caattctcta
     1621 aggcacacca gggggtccat ctgctgccag aagaggtgcg aggggtcgaa gttcaccccg
     1681 atagtctcgc cgcctatctc cctcagccgc cagaaagaat ccgtgttgta ggcgacgaaa
     1741 ccgggatgaa gctcgatagc gactttgacc ccgtgctcac cagcgaaaga cgcagcctcc
     1801 tgccagtacg gctcgacttt ctcgcgccac tgccactcga gggtctcgag gaagtcgggc
     1861 ggccaggggc aagtgaccca gttcggacgc ttcgagccct cggaatcgcc cgggcagccg
     1921 gagaaggtta tgacgctccc gacccctagc tcggcggcga gcctcaccgt gtcgcggaag
     1981 agttcgtcgt gctcccgggc catctccccg tcggggtgga gcgggttgcc gtggcacgag
     2041 agggcgctta tctccagatc cctgctctcg acggccttcc gaaactcgtc gagggctccg
     2101 gagtctccca gaagctccct cggtttgcag tgggcatccc cggggtagcc tccggttccg
     2161 atctcgaccg cctcgcagcc tgcctccctg acgtagtcga gcgcttctgc gagcggacgg
     2221 tccgccagaa gcaccgtgaa aaccccgacc ttcattctcc ctccctcgtt atgttccgtc
     2281 tcgagtcact cctggcccat cagagcccgc aggtgccgct ctgcgggttc gagtcgagct
     2341 tctcaatctg agattactac gaagccactt cctgtagctg gcgctcgagg tctttgatct
     2401 cgtccctgag ctttgcggcg tactcgaact tgagctcctc ggctgcttcg agcatctcgg
     2461 cctggaggtc ggcgatgagg ttctgcagct cgtcggggtc gcgaggggtc tcgcgggcgc
     2521 tcctcttgtc agccttgtag agacccttgg cttcggcggc gatcaggatg tcggagacgc
     2581 ccttcactat ggtgcgcggc tctatatcgt tctgctcgtt gtaggcgacc tggatctcgc
     2641 gcctgcggtt agtctcccct atggcgatcc gcatcgcctc ggtctcttta tcggcgtaca
     2701 tgatgacccg cccgttcacg ttgcgggcgg cgcggccgat ggtctggatg agcgcccgct
     2761 ccccacgcag gaaaccctcc ttgtcagcat caagtatggc gacgagggtt acctctggaa
     2821 ggtcgagacc ttcacgcagg aggttaatgc ctaccagtac gtcgaactcg ccggtacgga
     2881 ggccgcggat gatctggatg cggtccagcg tctcgatgtt ggcgtgcatg tagcgcgcct
     2941 tgaccccgtg ctcgagcagg taatcggtaa ggtcctcggc catctttatg gtgagcgtgg
     3001 tgatgaggac ccgctcgttt cgctcggtgc gcttcgcaac ctcgttcagc agatcgtcga
     3061 tctggttctg cgtcgaacgt acctcgacct cggggtccac caggccggtt gggcggatga
     3121 tctgctcgac tatctgctcg gagttctcga gctcgtaggg tcccggcgtc gccgagacga
     3181 agaccatctg gttagtcttg aggaggaact catcccacat gagcggcctg ttgtccaggg
     3241 cgctcggcag acggaaaccg aactcgacga gggtggtctt gcgcgagcgg tcgcctgtgt
     3301 acattccccg cacctggggc agcgtgatgt gcgattcgtc cacgaaggtg aggtagtcgt
     3361 cggggaagta gtcgagcagc gtgtacggcg tcgagcccgc agcgcgcccg tcgaggtggc
     3421 gcgagtagtt ctcgatgccg gagcagtagc caacctcacg catcatctcg aggtcgtacc
     3481 gggtgcgctg cctgagacgg taggcttcga ggaccttgcc ttcccgctcc agctcggtgg
     3541 tgcgctcctc gagctcggcc tcgatattcc tcacggcgag cgtgagccgc tcctcgtccg
     3601 tgacgaagtg ggtagccggg tagacggtta gagtgtccag ctcgcgcagg atctcaccgg
     3661 taagcgggtc cacctcggtc atgttctcga cctcgtctcc gaagagagag atcctgtaaa
     3721 tggtgtcctg gtatgccggg tggacctcga gcacgtcccc gcgaactctg aaggtgccgc
     3781 gggcgagctg gaagtcgttc ctggtgtact ggatacgtac caagtccctc aaaacgtcgt
     3841 cgaggtcgta gaagccgccc ctctcgagga ggaccatctt ggagcggtac tcatcagggc
     3901 tgccgaggcc gtagatcgcc gagacgctgg cgacgactat gacgtcgcgt ctggtgaaga
     3961 ggctgctcgt cgtggagtgc ctaagacggt ctatgtcctc gttgatctgg gcgtccttct
     4021 cgataaacgt gtcggtccgc gggacgtagg cctctggctg gtagtagtcg tagtacgaga
     4081 cgaagtactc gacggcgttg ttcgggaaga actcggagaa ctcgctcgcg agctgcgcgg
     4141 cgagcgtctt gttgtgcgcg atgacgagcg cgggacggcc cgccttctcg atgacacgcg
     4201 ccatcgtgtg ggtcttgccg ctccccgtaa cgccgagcag ggtctgggcg cgcatcccgg
     4261 cgtccaggcc cgaggcgagc ctctctatcg cctgcggctg gtcgccggta ggccggtatt
     4321 cgctgtttac cttgaagtta ctccgagttt cgatacaggt tccttccgta caattcttcg
     4381 tatacgctct gggcctctag gacgttctgg acgtagtggt gagtctcctt gaaggggatg
     4441 tcctcgccga cgtcgaagcc tttgtcggag atccaggcgt ccacccggcc ctcccctgaa
     4501 ttatacgccg cgagcacgag cctctcatcg cccatatacc ggccatcgag ataactcagg
     4561 taccaggccc ccatcctgag gttcaccttg gggtcgcgat accggccctc tatgccgctc
     4621 cgctcctgga tgaacctggc cgtctccggc aacagctgca tcaagccata ggccccctgg
     4681 tgcgacttgg cgtcaggcct gaagcggctc tcggtgtaga ccaccgcggc gacgaaggta
     4741 ggctccagac cgtactcggc gctcacatcc ctgatcgtct cctcgtagcg cagcgggtag
     4801 acggtccgcc ggacggcgtc gggggcctgc cggatcaggg gcagcaccgc gagcagcgcc
     4861 gcgatccctg cagccagcac tagcagtacg aggaccagaa gcctgcgccg ggcccgtgac
     4921 ctgccgtacc tactcttctt gctcgaata
//
LOCUS       DASTUV010000218         9663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_184655, whole genome shotgun sequence.
ACCESSION   DASTUV010000218 DASTUV010000000
VERSION     DASTUV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184655"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFT03_04360"
     CDS             complement(<1..510)
                     /locus_tag="VFT03_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847408.1"
                     /translation="MPPAGERERAPRTPRDPASEYRAKVAHELSRLSRLIKESGHPAP
                     LGDPASGVMLVVEQPVGPRVTQALQRSLSAVGLPEAYVTYASTGLLAQEILAAEPHAL
                     VAVGPGAARDVDALEHPLAQYPFSTAESGTWFAWTKGTAGLSLPALAPALEDDAAKRR
                     FWRAFLTLRD"
     gene            complement(494..1096)
                     /locus_tag="VFT03_04365"
     CDS             complement(494..1096)
                     /locus_tag="VFT03_04365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU4847409.1"
                     /translation="MSLPHREPREPFSLKDALRQLLHPLVRLLSALRVRPDTLTVLGW
                     TLSVGSAVLFGLGYTRIAGVVMLLGGLFDALDGAVARESNRMSDFGAFLDSTLDRLSE
                     ATIFVGIIFFYAAAARPYEALLAGVAMTFSLLTSYARARAEGLGMECQVGLLERAGRV
                     VILSAFSIAGLLTVGLGLVAAGALITTAQRILHVRRATRG"
     gene            complement(1093..2286)
                     /locus_tag="VFT03_04370"
     CDS             complement(1093..2286)
                     /locus_tag="VFT03_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4847410.1"
                     /translation="MPAKKLVVSTYLQYILIAVGFFLLVVFVRQLSGVLLTFLMAAIL
                     AYILNPVVRRLEQLGVPRIVAVLGVFFVLLLAVAAALLVLIIPAIGQGQTLVQNPDAV
                     IRRANALLGRAHEVPYVGERISELDEQEITQFLQSNAPSAGQVLRGATGFIGGIFGVF
                     GTVLNLVLMLIISIYLLLDRERVTRATLQAVPEMVRDHAVELFHAVESTLIRYLKGQF
                     ILCAIMGVIGWAIAFFVIGRYSLVLGLWVGLTEIIPVLGAFLGAVPAVLVALIVPGGG
                     FVKALIVAGLFLVAQQLEGNILVPRIQGGNVGVHPLWVLFATLAGTALYGLVGAIFAV
                     PLVAIIAATLRYLRGTVIFERWGKPPVVPVAEEREVLAVETSPSASVATGGSEEREPA
                     KGMEP"
     gene            2405..3232
                     /locus_tag="VFT03_04375"
     CDS             2405..3232
                     /locus_tag="VFT03_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847411.1"
                     /translation="MQRSRPVVRQRREAAPSSRKRTVPPQRDGRRAVALVERREARKA
                     NKAGVYRRRRVMAAGLLLSLVLALVFAVLAGTPEAAERAVPIDPGNAAPDTVLAEAAG
                     VEISTPIRPESLTGLGYHPEGESLVEMAPRGENLSANALIGLFADGSTPEDIHYHVMD
                     AADRTGPRTGALDVGAQAGARVYAPVTGMITAIRPDPMVQGANVVEIKPDTNANVRVT
                     VSLVQSDEANAGVTSRVTAGMTELGTVADSAEILDPQLSSYISDAGNHVTISVPRVG"
     gene            3269..3907
                     /locus_tag="VFT03_04380"
     CDS             3269..3907
                     /locus_tag="VFT03_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4847412.1"
                     /translation="MFLAIFQQSPAAAVALLLGLVVGVVVHEAAHAYSAYLLGDDTAY
                     RAGRVTLNPAAHLDLLGSMMFLMAGFGWGKPTPVVTSKLRGGVLGPVAVAAAGPLSNL
                     LIVAVCAALYLLPPFQSGYLQVIVLLAAFGNALLFVFNLIPIPPLDGAKVIFPFLPRS
                     LSGFVDFMNQYGPMILLVLVLLTFLPMLPSPLLYILVLVRPVLSAFGFPPIL"
     gene            complement(4162..5988)
                     /locus_tag="VFT03_04385"
     CDS             complement(4162..5988)
                     /locus_tag="VFT03_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4847413.1"
                     /translation="MFSARKRTFGEKPTPWRKMRRRLSSALKTGFKPRIGMRIWLTAM
                     FVMVTAFAAVVAYETVRPILEDTLDRASQARFEEVAQQFENEIEKNQNFSLQRIRTFA
                     DIRGLQWGIVSAKTGTKLRGDRDLDEWHQGVVQNAVKTQSPKMKIEPVRSGRRTGQIR
                     ATYAVPINAEAELGVDGTAVVFSEFFTESDIENVQKALESIQRIALLAGALALALAGV
                     VGYLAASLISRRISRLGVAAERLAAGNFDERIETRVEDEVGSLGATFNTMAASLKGAF
                     EQIRHEKERGSAILNGMTDAVVGVDKDLNAVFLNPRARELLESSDLDFQIRLQEVLAK
                     TRYSGPITEPEAMAGDRIIEIRAAPLEDGALAILRDVTEERRVQRAKAEFIANASHEL
                     KTPLTALSGYLEMLEDEQDERVRSEFLKDMRSQTDRLQSLARTLLDLSRLDANATTFR
                     TEEVDLEDILHELRRDFGYTGRPMSIHADDVPPVVTDPIQLHRMLAILLDNALKYSSE
                     DTPIDLDLSRDDGHAVISVTDRGCGIPESEIPHIFDRFYRAQGSSRADGTGLGLALAR
                     EITDHLGGNIQVQSHPGTGSTFSVALPLTADSRDGSRSSSLS"
     gene            complement(5975..6652)
                     /locus_tag="VFT03_04390"
     CDS             complement(5975..6652)
                     /locus_tag="VFT03_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4847414.1"
                     /translation="MTRRILVVDDEPSLQKMLAHALEREGFQVQTVGDGEEALEAFKS
                     YEPHLIILDIMLPKLDGTEVCRRIRAQSDVPIIMLTAKDDEIDRVVGLELGADDYVTK
                     PFAVRELVARVRAIMRRAAVPAGQRPDELNYDGLRINLPSRRVSVANEEVDLTYTEFE
                     LLVTLASSPGRVFSRSALLRRVWGDEFRDERTVDVHIRHLREKIERDPRNPEFIHTAR
                     GVGYVFR"
     gene            complement(6727..8529)
                     /locus_tag="VFT03_04395"
     CDS             complement(6727..8529)
                     /locus_tag="VFT03_04395"
                     /inference="COORDINATES: protein motif:HMM:NF005906.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEU4847415.1"
                     /translation="MSPSLYGFVRGREPVSSEEVAAEFLSLPNPNGDARARVEALIGE
                     DPRFLWEGEALVTADPASLALDEAPYVVFDIETSGSSAAKGGAITELGALKVVRGEVV
                     EEFATLVNPGRKIEPFVARLTGITDRMVAKAPPISEVMPRFERFVEGSVLVGHNVHFD
                     CSFVTAARGLPLPNPVLDTLKLARCLVPGLRRYRLGSLASHFGVLAMPNHRALSDAAA
                     TAGVFLKLLKLLRSAGVLSVGEAATLRGGGRVKPQKQHLAEGIPSTPGVYYFIDKHGT
                     TLYVGKAKNLRARVRTYFNGGDGRRKIGRLVEEVAEVRVRETESELQALILEAREIKR
                     LLPRYNSAGRDERAGWFIRLDVSEPYPVPERVPANGREEGAVHLGPYRSAGMLDACIE
                     AMGRIFPLKRCSGRNGVCFYGQLGRCGPCIGMNEEDYRRLVVDEVVALLRGEGGEEHL
                     AALVRERERLAAALEFEAAARLRDLIAGIERIRLTRSLVSAEGLQAVVAASTEPEIVE
                     VFALSEGHLVAHRGFGVGDLAGLSSFAEEVLAGCEEVGGGGEATRTADEARIVAAYLR
                     RRSAAMEAVRLGEARDLLEAVSRVVGDGATSSSC"
     gene            complement(8519..9553)
                     /locus_tag="VFT03_04400"
     CDS             complement(8519..9553)
                     /locus_tag="VFT03_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4847416.1"
                     /translation="MFESEFEDNTFRRRLMKEVAPGVPVHFSFYSPSVKVQVQPFAEH
                     LPSANGRYVGDAVTIEFVPEDAAAVERVREFVWCWEEHNAFTGVRKVNYLRRGVCPES
                     VQGMLERVRGVDKRAAAKAAEAVGYEDLRDLIAEKDAKTLARLPGLGERRARAVVEFY
                     KNEESDRLFLYAANRNDRFRGRPVVTELDLDGDLEDQVFDHAARARALGDPDPLGSAE
                     PPGHTTVRNANLLHPLPMTPSLMSRGVFYPEQIELFADTCRRVLVEGERLSGVGSLRI
                     QRGKLFHTTRMPGVGSKTRARILASGLLDDEYTYENLAAIQGVTEAQARTIAEAARIE
                     AGARTEVRVP"
BASE COUNT         1600 a         3259 c         3203 g         1601 t
ORIGIN      
        1 atcccgcagg gtaaggaagg cccgccagaa gcgtcgtttg gcagcgtcgt cttcgagggc
       61 gggggcgagg gcgggcaggc tgagtcctgc ggtgcccttt gtccaggcga accacgtgcc
      121 gctctcggcg gtcgagaagg ggtattgtgc gagtgggtgt tcgagcgcgt ctacgtcgcg
      181 ggccgcgccg ggaccgacgg cgacgagggc gtggggctcg gcggcgagga tctcctgggc
      241 gagcaggccc gtggaggcgt aggtgacgta agcttcgggg agtccgacgg cactcaggct
      301 gcgctggagg gcttgcgtga cccgggggcc gaccggttgc tcgacgacca gcatcacccc
      361 tgatgctgga tctccgagcg gggccgggtg gcctgactcc ttgatcagcc gggacaggcg
      421 ggagagctcg tgcgcgacct tggcgcggta ctcgctggcc gggtcgcgtg gcgtgcgggg
      481 ggcacgctcc ctctcacccg cgggtggcac gacggacgtg gagtatgcgc tgggctgtag
      541 ttatcagggc accggcggcg accaggccga ggccgaccgt cagcagcccc gcgatcgaga
      601 aggccgagag gatcacgacc ctgccggctc gctcgagcag gcccacctgg cactccatac
      661 ccagtccttc ggcgcgcgcg cgggcgtatg aggtgagcag ggagaaggtc atggcgacgc
      721 cggcgaggag ggcctcgtag ggtcgggcag cggcggcgta gaagaagatt atgccgacga
      781 agatggttgc ctcggagagc cggtcaaggg tcgagtcgag gaaggcgccg aagtcgctca
      841 tccggttcga ctcgcgggcg accgcgccgt cgagggcgtc gaagaggccc ccgaggagca
      901 tcaccacgcc cgcgattcgg gtgtatccga gcccgaacag cacggcggag ccgaccgaga
      961 gcgtccagcc caggacggtg agcgtgtcag ggcgcactcg cagggcggag agcagccgga
     1021 cgagcgggtg caagagctgg cgtagggcgt ctttaaggga gaacggttcg cgcggctcgc
     1081 ggtgcggaag actcaaggct ccattccttt ggccggttcc cgctcctccg aaccaccggt
     1141 agccaccgac gcagagggcg aagtctcgac cgccagtacc tccctctcct cggccacagg
     1201 gacgacggga ggcttgcccc agcgctcgaa gatcacggtg ccgcgcaggt agcggagcgt
     1261 agcggcgatg atggcgacca gggggacggc gaagatcgcg ccgacgaggc cgtagagggc
     1321 tgtccccgcg agggtggcga agaggaccca cagagggtgc acgcccacgt taccgccctg
     1381 gatcctcggc accaggatat taccttcaag ctgctgggcg acgaggaaga ggccggcgac
     1441 tattagagcc ttgacgaaac cccctcctgg cacgatgagc gcgaccagaa ccgccgggac
     1501 ggcgccgagg aaggccccga ggacggggat gatctccgtc aggccgaccc acagcccgag
     1561 cacgagcgag taccgtccga tgacgaagaa agctatggcc cagcctatga cgcccattat
     1621 cgcgcagagg atgaactgtc ccttgaggta ccggatcagg gtactctcga cggcgtggaa
     1681 gagctcgacg gcgtggtcgc gcaccatctc cgggaccgct tgaagcgtgg cacgggtgac
     1741 cctctcccgg tcgaggagga ggtagatgga gattatgagc atcagcacca ggttcaacac
     1801 ggtgccgaag acgccgaaga tgccgcctat aaagccagtt gctccccgga gcacctgccc
     1861 ggcggaggga gcgttgctct ggaggaactg cgtgatctcc tgctcgtcca actcggagat
     1921 gcgctcgccg acgtagggca cctcatgagc gcgccccagc agcgcgttcg cccgccgtat
     1981 gacggcgtcg ggattctgga ccagggtctg tccctgtccg atggcgggga tgatgagcac
     2041 gaggagcgcg gcggcgacgg cgagaaggag cacgaagaac acgcccagca ccgccacgat
     2101 cctgggaacg ccaagctgct cgagtcgacg caccacgggg ttgaggatgt aggccaggat
     2161 ggcggccatg aggaacgtca gcagaacacc gctgagctgc cggacgaaga caaccagcaa
     2221 gaagaagccc acggcgatca ggatgtactg taggtacgtc gagacgacga gctttttggc
     2281 gggcaccctg gtctccgatc gcggactgta cagcccaaat ataccagtcc ctggcgccgg
     2341 ggcggcaagt ttacagccca cgagcccctc tgtataatcg tcccccagta tgcgaggggg
     2401 taacatgcag aggtctcgac ccgtcgttag gcagaggcgt gaggcagccc cgtcgagccg
     2461 caagcgcaca gttccaccgc agcgggacgg ccggcgcgca gtcgccctcg tcgagcgccg
     2521 cgaggcccgg aaagcgaaca aagccggcgt ctaccgcaga aggcgcgtca tggccgcggg
     2581 cctgttgctc tccttggttc tggcgctcgt cttcgcggtg ctcgcaggga ccccggaggc
     2641 ggcggagcgc gcagttccca tagacccagg caacgccgcc cccgacacgg tgctggccga
     2701 ggccgctggc gtcgagatct ccactcccat caggcccgag agcctcaccg gcctgggcta
     2761 ccatccggaa ggcgagagcc tggtcgagat ggcgccccgc ggcgagaacc tctccgcgaa
     2821 cgcgctcatt ggcctcttcg cggacggatc tacgcccgag gacatccact accacgtcat
     2881 ggacgcagca gaccgtacag gcccccggac cggcgccctc gacgtcggag cccaggcggg
     2941 ggcgagggtc tacgctcctg taaccggcat gatcacggcc atacgccccg accccatggt
     3001 gcagggggcc aacgtcgtcg agatcaaacc cgacaccaac gcgaacgtcc gagtcacggt
     3061 ctcactcgtg cagagcgacg aggcaaacgc gggcgtcacg tcccgcgtga cggcagggat
     3121 gaccgagctc ggcaccgtgg ccgactcggc ggagatactg gacccccagc tctcctccta
     3181 tatctccgac gcgggcaacc acgtcaccat ctcggtcccg cgggtcggct agagtccagt
     3241 cccccggcgg ggcgtgtatc ataagcgggt gttccttgcg atcttccaac agagccccgc
     3301 cgccgcggtg gccctcctgc tcggcctcgt cgtcggcgtc gtggtgcacg aggcggcgca
     3361 cgcctactcc gcctacctcc tcggcgacga caccgcttac cgcgccggca gggtgacgct
     3421 caaccccgcc gcccacctcg acctgctcgg gagcatgatg ttcctcatgg ccggcttcgg
     3481 ctggggcaag ccgacacccg tggtcacctc caaactcagg ggaggcgtgc tcggccccgt
     3541 cgccgtagcc gccgccggac ctctctcgaa cctcctgatc gtcgccgtct gcgccgccct
     3601 ctatctgctg ccgcccttcc agagcggata cttgcaggtc atcgtgctgc tggcggcgtt
     3661 cggcaacgct ctgctcttcg tcttcaacct gatccccatc cccccactcg acggcgcgaa
     3721 ggtgatcttc ccgttcctgc cgcgctccct gagcggcttc gtggacttca tgaaccagta
     3781 cggaccgatg atactgctgg tgctcgtgct cctcacgttc ctgcccatgc tcccctctcc
     3841 cctcctctac atactggtcc tcgtgcgacc ggtgctatcc gcgttcggct ttcccccgat
     3901 tctctaatgg gctacaggta tcaggtggtg cttcctctgc gagataaccc gtcgtgggtg
     3961 gggaaagtca tctataaatt cgtatcagta gcggaaccac gatctgccga gcaggaaaaa
     4021 gctgaagcct gagacctgac ccagaaataa ataggggcag cgacggtccg cagggagggg
     4081 ggagaatcta cgaacctacg ctaccccagc ccaattctac cgccagaaat ccagaaacgt
     4141 agattgaacc tcaagtacag cctaagacag gctcgaggag cggctaccgt ccctggaatc
     4201 cgccgtcagg gggagggcga ctgagaaagt gctacccgtt cctgggtggc tctggacctg
     4261 gatattgcct cccaggtggt ccgtgatctc gcgcgccagg gcgaggccca gccccgtgcc
     4321 gtcggcgcgg gaggagccct gagcgcggta gaagcgatcg aagatgtggg gtatctcgct
     4381 ctcggggatg ccgcagccgc ggtcggtgac gctgatgacg gcgtggccgt cgtcgcggga
     4441 gaggtcgagg tctatagggg tatcctcgct agagtacttg agggcgttgt caagcaggat
     4501 ggcgagcatc ctatgtagct ggatcgggtc ggtcacgacg ggcgggacgt cgtctgcgtg
     4561 gatgctcatg gggcggcctg tatagccgaa gtcccggcgc agctcgtgga ggatgtcctc
     4621 gaggtcgacc tcttcggtgc ggaaggttgt ggcgttcgcg tccaggcggg agaggtcgag
     4681 caacgtgcga gccaggctct ggagcctgtc ggtctgggag cgcatgtcct tgaggaactc
     4741 ggacctgacc cgctcgtcct gctcgtcctc gagcatctcc aggtagccgg agagggcggt
     4801 caagggcgtc ttgagctcgt gggaggcgtt ggcgatgaac tcggccttcg cgcgctgcac
     4861 gcggcgctcc tccgtcacgt cgcgcaggat ggccagagcg ccgtcctcga gaggggcggc
     4921 ccggatctcg atgatcctgt cccccgccat tgcctcgggc tcggtgattg ggcctgaata
     4981 gcgcgtcttg gcgaggacct cttgaagccg gatctggaag tccaggtccg aggactcgag
     5041 gagctcacgc gcgcgcgggt tcaggaagac ggcgttgagg tccttgtcca cgccgacgac
     5101 agcgtctgtc ataccgttga gtatcgcgct cccgcgctcc ttctcgtgcc tgatctgctc
     5161 gaaggcgcct tttagggagg cggccatcgt gttgaacgtc gcgcccaaag agcccacctc
     5221 gtcctcgacg cgggtctcta ttcgctcgtc gaagttgcct gccgccagcc tctcggcggc
     5281 caccccaagc cggctgatac ggcgcgatat caggctagcc gccaggtacc ccacgacccc
     5341 ggcgagcgcc agcgccaggg cgccggcgag cagggctatg cgctgtatgc tctccagcgc
     5401 cttctgcacg ttctcgatgt cgctctcggt aaagaactcg ctgaagacca ccgccgtccc
     5461 gtccacgccg agttccgcct ccgcgtttat cggaacggcg taggtggccc ggatctggcc
     5521 cgtgcgacga cccgacctga ccggctcgat cttcatcttc gggctctggg tcttgaccgc
     5581 gttctgcacg accccctggt gccactcatc gaggtcccgg tcgcccctta gcttggtgcc
     5641 ggtcttcgcg ctcactatgc cccactgcag gccccggata tcggcgaagg tccggatacg
     5701 ctgcagcgaa aagttctggt tcttctcgat ctcgttctcg aactgctggg ctacctcctc
     5761 gaagcgggcc tggctcgccc ggtcgagcgt atcctcgagg atgggacgga cggtctcgta
     5821 ggccacgacc gccgcgaagg cggtcaccat gacgaacatg gccgtgagcc agatgcgcat
     5881 gccgatgcgc ggcttgaacc ctgtcttcaa agccgaggag aggcgcctcc tcatcttgcg
     5941 ccagggagtg ggcttctccc cgaaggtcct cttcctagcg gaaaacatac cccacgcccc
     6001 gcgcggtgtg gatgaactcg gggttgcggg ggtcgcgctc gatcttctcg cgcaggtggc
     6061 ggatgtggac gtcgaccgtg cgctcgtcgc ggaactcgtc gccccacacc ctcctcaaga
     6121 gcgcgctgcg cgagaacacc ctgccggggc tcgacgcgag cgtcacgagg agctcgaact
     6181 cggtgtaggt gaggtccacc tcctcgtttg ccactgacac ccggcggctc gggaggttga
     6241 tgcgcagccc gtcgtagttg agctcgtccg ggcgctggcc cgccggtacc gcggcccggc
     6301 gcatgatcgc ccgcaccctc gccaccagtt cccgcacggc gaacggcttg gtgacgtagt
     6361 cgtcggcccc gagctccagc cccacgacgc ggtcgatctc gtcgtccttg gccgtgagca
     6421 tgatgatggg cacgtcgctc tgcgccctga tgcgcctgca cacctccgtc ccgtccagct
     6481 tggggagcat gatgtccagg atgatgaggt gcggctcgta gctcttgaac gcctcgaggg
     6541 cctcctcgcc gtcgccaacc gtctggacct ggaagccttc ccgctccagg gcgtgggcca
     6601 gcatcttctg tagcgacggt tcgtcgtcta ccacgagaat cctgcgggtc acgctctgta
     6661 aaccttgcac catactctca tgccctcttt gcatgtctgg atatcgccaa cgcgcttggg
     6721 cttattttag catgatgaag aggttgcacc gtctcccacg actctgctca cggcctcgag
     6781 gagatcccgc gcctctccca gccgcacggc ctccatggca gccgagcggc gcctgaggta
     6841 cgctgctacg atccgcgcct cgtcggcggt acgggtagcc tcccccccgc cgcctacctc
     6901 ctcgcatcca gccaggacct cttcggcgaa gctcgagaga ccggcgaggt ccccgacccc
     6961 gaacccccgg tgggcgacga gatggccctc cgagagggcg aatacctcga ctatctcagg
     7021 ctcggttgag gctgccacta cggcctgcaa cccctcggcg ctcacgaggg agcgcgtcag
     7081 gcgtatccgc tctatcccgg cgatgaggtc cctgaggcgc gcggcggcct cgaactcgag
     7141 cgccgctgcg agacgctccc gctcccggac cagggccgcc aggtgctcct cgccgccctc
     7201 gccgcgcagc agagcgacca cctcgtccac gaccaggcgg cgataatctt cctcgttcat
     7261 gccgatgcac ggcccgcagc ggccaagctg gccgtagaag cagacgccat tccgcccgga
     7321 gcatctcttg agggggaaga tcctgcccat agcctctatg caggcgtcga gcattccggc
     7381 gctccggtac ggcccgaggt ggacagcgcc ctcctcgcgc ccgttcgcgg gcacccgctc
     7441 ggggacggga tatggttcgc tcacgtcgag ccttatgaac cagccggccc tttcgtctcg
     7501 gcccgcggag ttgtagcggg ggagcaggcg ctttatctcc ctggcctcga ggatgagggc
     7561 ctgcagctcg ctctcggtct cccgcacgcg cacctcggcg acctcctcga ccaggcggcc
     7621 tatcttgcgg cgcccatcac cgccgttgaa gtacgtgcgg acccgcgccc gcaggttctt
     7681 ggccttcccg acgtagagcg tggtgccgtg cttgtcgatg aagtagtaga cgccgggcgt
     7741 ggaagggatg ccctcggcga ggtgctgctt ctgcggcttg acgcgtcctc cgcccctcaa
     7801 ggtcgccgcc tctcccacgc tgagcacccc ggcggagcgc agcagcttca ggagcttcaa
     7861 gaatactccg gctgtcgccg ccgcgtccga gagggcgcgg tggttaggca tggccaacac
     7921 gccgaagtgg gaggcgagcg atccgagacg gtagcgcctg aggcccggca ccaggcagcg
     7981 cgcgagcttt agggtgtcga gcaccgggtt cgggagcggg aggccgcggg cggcggtgac
     8041 gaaggagcag tcgaagtgga cgttgtgccc gacgaggacc gagccctcga cgaaccgctc
     8101 gaaacggggc atgacctccg agatgggcgg cgccttggcc accattcggt ccgtgatgcc
     8161 ggtcaggcgg gcgacgaagg gctctatctt gcggccgggg ttgaccaggg tggcgaactc
     8221 ctccaccacc tcccctcgca ccactttcag ggcgccgagc tccgtgatgg cgccaccctt
     8281 cgccgccgac gaccccgagg tctcgatgtc gaagacgacg tagggggcct cgtccagcgc
     8341 gagagaggcc gggtcagccg tcaccagggc ttccccttcc cagaggaagc gtgggtcttc
     8401 accgatcagc gcctcgaccc gggcccgcgc gtcgccgttc gggttgggga gggagaggaa
     8461 ctcggcagcg acctcctcgg agctcacggg ctcgcggccc ctgacaaacc cgtagaggct
     8521 aggggacacg gacctccgtc cgcgcgcccg cctctatgcg cgccgcctcg gcgatggtcc
     8581 gggcctgcgc ctccgtcacg ccctggatgg cggccaggtt ctcgtaggtg tactcgtcgt
     8641 cgagcagacc gctcgccagg atgcgcgccc gcgtcttcga gccgacgccg ggcatcctgg
     8701 tagtgtggaa gagcttgccg cgctggatgc gtaaagagcc gaccccagat agccgctcgc
     8761 cttcaacgag gacgcgccgg cacgtgtcgg cgaagagctc gatctgctcc gggtagaaga
     8821 cgccccggct catgagggag ggcgtcatgg gcagcgggtg caagaggttg gcgttgcgta
     8881 ccgtcgtgtg tccgggcggc tcggccgaac caagggggtc agggtcgcca agagcgcgcg
     8941 cccgcgccgc gtggtcgaag acctgatcct cgagatctcc gtccaggtcc agctccgtga
     9001 ccacgggcct gccgcggaag cggtcgttgc ggttggccgc gtagaggaag agcctgtcgc
     9061 tctcctcgtt cttgtagaac tcgaccacag cgcgggctcg cctctcgccg agacccggca
     9121 accgcgccag cgtcttggca tccttctccg ctatgaggtc tcgcaggtct tcgtagccca
     9181 ccgcctcggc cgccttggcc gcggcccgct tgtccacgcc gcgcaccctc tcgagcatac
     9241 cctgcacgct ctcagggcag acgccgcgcc tgaggtaatt gaccttgcgc acccccgtga
     9301 acgcgttgtg ctcctcccag caccagacga actcccgcac ccgctcgacc gccgcggcgt
     9361 cctcggggac gaactcgatc gtcaccgcgt cgcccacgta gcggccgttg gcgctgggca
     9421 ggtgttcggc gaagggctga acctgcactt tcaccgaagg cgagtagaac gagaagtgca
     9481 ccgggacgcc cggcgcgacc tccttcatca agcgcctgcg gaaagtgttg tcttcgaatt
     9541 ctgactcaaa catcgggacc gtattctacc accgccaccc cctgaaccat cggcactctg
     9601 tgaccttcga tcacaggatc acaccttctc gcaacacgat ggcctctacc gtctccccag
     9661 ccc
//
LOCUS       DASTUV010000219         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_28690, whole genome shotgun sequence.
ACCESSION   DASTUV010000219 DASTUV010000000
VERSION     DASTUV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28690"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /gene="rho"
                     /locus_tag="VFT03_04405"
     CDS             <1..1404
                     /gene="rho"
                     /locus_tag="VFT03_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU4847417.1"
                     /translation="EQYRKYRKPELAQMIYKVATEQAAEQAADREVGAVATATEPQTT
                     NGETGVATIPESAADDTAFGSAVDAAVEAPAEQRQPRQRQEPRQRQERGDRGEELIQS
                     GVLDVLPDGFGFVRTNGYSQSKGDVYVSTSQIKRFNLRRGDYIVGQIRPARDGEKYPA
                     MVRIETINGKEPQKGRWRPNFDDLTPLYPMERLRLEWKPNDIAPRVIDLVAPIGKGQR
                     GMVVSPPKAGKTTVLKQIAQSIAANYPETKLFVVLADERPEEVTDWERSVEEAEVIAS
                     TFDQPADNHIAVAELVLERVKRLVEEGEDVVVLLDGITRLSRAYNLAAPASGRILSGG
                     VDSAALYPPKKFFGAARNIENGGSLTILATALVETGSRMDEVIFEEFKGTGNMELRLD
                     RKLANRRLYPAINIEDSSTRKEELLMEPAEAQRVWQVRSILNTLEPSAKLELLIAKLK
                     ETRTNAEFLRELQRVRG"
     gene            1501..1758
                     /locus_tag="VFT03_04410"
     CDS             1501..1758
                     /locus_tag="VFT03_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015650714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU4847418.1"
                     /translation="MKKGIHPEYRPVVFRDGSADFAILTRSTIETERTIEWEDGNTYP
                     LVELDISSASHPFYTGRQRIVDTGGRVQRFENRRQSRQKRQ"
     gene            1768..>2509
                     /locus_tag="VFT03_04415"
     CDS             1768..>2509
                     /locus_tag="VFT03_04415"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEU4847419.1"
                     /translation="MRVGGQAIMEGVLMRSPNFWGIAVRTPTGELDLRAEGFRSITRR
                     SRLFRLPIIRGALSLGETLWLGMKALTLSTNIALGEEEDLSKREIAFTLGFAAVLGFG
                     LFLVAPVLGTKGIGALLGNSIEHPVLFNLVEGAIRIAIFVAYLIGITLISKDVKRFFA
                     YHGAEHKAIKVYERGEELVPENARGLDTSHVRCGTSFVLYVLVLSILVFSLLGVEGWL
                     FMVLSRLIVIPLVAGIAFEFIMWSARHQE"
BASE COUNT          500 a          789 c          827 g          393 t
ORIGIN      
        1 gagcagtacc gcaagtaccg caagcccgag ttggcgcaga tgatctacaa ggtagccacg
       61 gaacaggccg ccgagcaggc ggccgacaga gaggttggag cggtagcgac cgcgaccgag
      121 cctcagacga ccaacgggga aacgggcgtt gccaccatcc ccgagtccgc cgcggacgac
      181 accgcctttg gcagcgccgt ggacgccgcg gtcgaagccc ccgccgagca acggcagccg
      241 cgccagcgtc aggagccgcg ccagcgtcag gagcgcggtg acaggggtga ggagctgatc
      301 cagtccggcg tcctcgacgt gctgccggac gggttcgggt tcgtccggac caacggctat
      361 agccagagca agggggatgt ctacgtctcg acgagccaga tcaaacgctt caacctcagg
      421 cgcggagact acatagtcgg ccagatacgg cccgctcgcg acggagagaa gtaccctgcg
      481 atggtgcgca tcgagaccat caacggcaag gagccccaga agggccgctg gcgtccgaac
      541 ttcgacgacc tgacgccgct ttacccgatg gagaggctcc gcctcgagtg gaagccgaac
      601 gacatcgccc cgcgcgtcat agacctcgtc gccccaatag gcaagggcca gcgcggcatg
      661 gtggtctcgc cgcccaaagc cggcaagacc acagttctca agcagatagc ccagtccatc
      721 gcagccaact accccgagac caagctattc gtggtgctcg ccgacgagcg ccccgaggag
      781 gtcacggact gggagcggag cgtggaggag gccgaggtca tagcctcgac cttcgaccag
      841 cccgcagaca accacattgc ggtggctgag cttgtcctcg agcgcgtcaa gcgcctcgtc
      901 gaggagggcg aggacgtggt cgtgctgctc gatggcataa ccaggctctc gagggcgtac
      961 aacctcgccg cgccggcgag cggcaggata ctctcgggtg gtgttgactc cgccgctctg
     1021 tatccgccca agaagttctt cggagcggcg cgcaacatcg agaacggcgg ctcgctcacg
     1081 atcctcgcca ccgcgctggt cgagaccggc agcaggatgg acgaagtcat cttcgaggag
     1141 ttcaaaggca cgggtaacat ggagctcagg ctcgatagga agctggcgaa ccgcaggctc
     1201 taccccgcca tcaacatcga ggactcctcg actcgcaagg aagagctcct catggagccg
     1261 gccgaggccc agagggtctg gcaggtccgc agcatcctca acacgctcga gccctcggcg
     1321 aagctggagc tcctcatagc caaactaaag gagacccgca ccaacgccga gttcctgcgc
     1381 gagctgcagc gcgtccgggg ctagcaagag ccatcctggc ggggcggcga tccgggccat
     1441 cccgccttta cagacgtatt agactagtat ggtggcgttt cattctagat ggaggaacgg
     1501 atgaagaagg gcatacaccc tgagtacagg cccgtggtct ttcgcgacgg gagcgcggac
     1561 ttcgcgatcc tgacccgctc gacgatagag acggagagga ccatagagtg ggaggatggg
     1621 aacacctacc cgctggtcga gctcgacatc tcgagcgcga gccacccctt ctacacgggt
     1681 aggcagcgca tcgtggacac cggcggccgg gtgcagcggt tcgagaacag gcgccagtcc
     1741 cgccagaaac ggcagtagga cccggccgtg agggtcgggg ggcaggccat aatggagggc
     1801 gtgctgatgc gctcccccaa cttctgggga atagccgtcc gcacgccgac gggagagctc
     1861 gatctcaggg ccgagggctt ccgctcgatc acgcgccgga gcaggctctt caggctcccg
     1921 ataatccgcg gcgctctctc gctgggcgag accctctggc tcggcatgaa ggcgctgacg
     1981 ttgtctacta acatcgcgct tggcgaggaa gaagacctct ccaagcggga gatcgcgttt
     2041 accctcgggt tcgcagccgt gctcggcttc gggctcttcc tggtggcgcc tgtgctcggg
     2101 accaaaggga taggcgcgct tctgggcaac agcatcgagc atcccgtgct cttcaacctg
     2161 gtcgagggcg cgatccggat cgctatcttc gtcgcctacc tgatcggtat aaccctgatc
     2221 agcaaggacg tgaagcgctt tttcgcctac cacggagccg agcacaaggc cataaaggtc
     2281 tacgagcgcg gtgaggagct cgtccccgag aacgctcgcg ggctggacac cagccacgtc
     2341 cgctgcggga ctagcttcgt actgtacgtg ctggtcttga gcatcctcgt attctcgctg
     2401 ctcggggtcg aggggtggct gttcatggtg ctgagcaggc tcatcgtgat accgctggtg
     2461 gcgggtatcg ccttcgagtt catcatgtgg agcgcgaggc accaggaga
//
LOCUS       DASTUV010000220         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_538651, whole genome shotgun sequence.
ACCESSION   DASTUV010000220 DASTUV010000000
VERSION     DASTUV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538651"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFT03_04420"
     CDS             complement(<1..217)
                     /locus_tag="VFT03_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847420.1"
                     /translation="MAIREEVPAESRNLVEMSWDPITRIVGNLGIYTKIDFENREVVE
                     CKSTSSIFRGYSVFMKGKDPRDAHFITS"
     gene            complement(230..1288)
                     /locus_tag="VFT03_04425"
     CDS             complement(230..1288)
                     /locus_tag="VFT03_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847421.1"
                     /translation="MTETQATSYGPGVEEVHILWISEGMSCDGDSVSVTAASLPSIED
                     VLLGNIPGLPQVHLHNKVLDPSLGGEDFLQVYRQAANDELDVPFVLVIEGSIPNENIN
                     GDGYWTSFGNDLATGEPLTLNWWIDQLAPRAWAVVAVGTCATYGGIHAMAGNPTGCMG
                     LADYLGWDFRSQAGIPIVNIPGCPVQPDNFMETLTWLLYQAAGMLPMIPLDEQLRPTW
                     LFGKTVHEGCDRAAYYEQGDFALDYNSPKCQVKIGCWGPVVNCNVTKRGWMDGVGGCP
                     NVGGICIGCTMPGFPDKFMPFMDEPPGSTFSTSLVMVYGAMIRRLRSITNETVNKEPK
                     WRHNKPELTTGYDPRWRA"
     gene            1585..1815
                     /locus_tag="VFT03_04430"
     CDS             1585..1815
                     /locus_tag="VFT03_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HEU4847422.1"
                     /translation="MANSVELYVTAGCPYSASAREDLEWRGVDFVEYDVEQDRDAYER
                     MLELTGGNRTVPVIVEEGKPVQVGWMGQGCFV"
     gene            1903..2370
                     /locus_tag="VFT03_04435"
     CDS             1903..2370
                     /locus_tag="VFT03_04435"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU4847423.1"
                     /translation="MEDRGRSAAGREAPETKDRYPHLLAVPTRWIDNDVYGHVNNVVY
                     YSYFDTVVNGYLISSGALDIEKSSVIGLVVETQCRYFKPITFPDAVHAGLRVARLGKS
                     SVRYEIGIFRNDEETAAAQGHFVHVYVDRENRRPTTLPPVMREALERVLLPEG"
     gene            complement(2377..2871)
                     /locus_tag="VFT03_04440"
     CDS             complement(2377..2871)
                     /locus_tag="VFT03_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4847424.1"
                     /translation="MRAQSMNSRDRSKLRAEVEALVASDAPDDEVLEEVVRLVHEAHP
                     RWDWSGIYLLVDGTLVVGPFAGSEEPPEHSRIPVGDGVCGTAVAQDRNQLVDDVRELD
                     NYLACSLSTRSELVVLIREDGRIVGQFDIDSDERGAFTQEDEALLEELATVVAPRVAS
                     LAAP"
     gene            complement(2935..>3152)
                     /locus_tag="VFT03_04445"
     CDS             complement(2935..>3152)
                     /locus_tag="VFT03_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847425.1"
                     /translation="ATDPDVLNIGRALAQMPARVRPDQIIGPPGWTSALGYGRQKGWI
                     LERDQLELHEDHLHLGYMDEQGTRNVR"
BASE COUNT          541 a         1040 c          970 g          601 t
ORIGIN      
        1 ggctggtgat gaagtgcgcg tcgcgcgggt ccttgccctt catgaagacg ctgtagccgc
       61 ggaagatgga agaggtgctc ttgcactcga ccacctcccg gttctcgaaa tctatcttgg
      121 tatagatccc gaggttcccg acgatgcggg tgatcgggtc ccatgacatc tccaccaggt
      181 tcctggattc tgccggaact tcctctcgga tagccactta tcctcctctc tacgcccgcc
      241 agcgcgggtc gtagccggtt gtgagctccg gcttgttgtg gcgccacttg ggttccttgt
      301 tgaccgtctc gttggttatg ctcctgagcc tccggatcat ggcgccgtaa accatgacca
      361 ggctcgtgga gaacgtggag cctggcggct cgtccatgaa cggcatgaac ttgtcaggga
      421 agccaggcat cgtgcagccg atacagatgc cgccaacgtt cgggcatccg ccgaccccgt
      481 ccatccagcc cctcttggtc acgttgcagt tgaccacggg tccccagcag ccgatcttga
      541 cctggcactt gggcgagttg tagtcgagcg cgaagtcgcc ctgctcgtag taggcggccc
      601 tatcacagcc ttcgtgcacg gtcttcccga agagccaggt cggcctgagc tgctcgtcca
      661 gcgggatcat cggcaacatg ccggccgcct ggtagagcag ccatgtcaaa gtctccatga
      721 agttgtccgg ctgcacggga caacccggga tgttcactat cggtatcccg gcctgagagc
      781 ggaagtccca tcccaggtag tctgccaacc ccatgcaacc ggtgggattt cccgccatcg
      841 cgtggatccc gccgtaggtc gcgcaggtcc cgactgccac cactgcccac gccctcggcg
      901 cgagctggtc gatccaccag ttgagggtga ggggctcgcc ggtagccaga tcgttcccga
      961 acgacgtcca gtacccgtcc ccgtttatgt tctcgtttgg gatggagcct tcgatcacca
     1021 ggacgaacgg gacgtcgagc tcgtcgttgg ccgcctggcg atagacctgc aggaagtcct
     1081 ccccgccgag cgaggggtca agcaccttgt tgtgcaggtg tacctggggc agccccggta
     1141 tgtttcccaa cagcacgtcc tcgatgctgg gcagcgaggc cgccgtgacg gagaccgagt
     1201 ccccatcgca gctcatgccc tcggagatcc agagtatgtg aacctcctct actcccggcc
     1261 catagctagt ggcttgagtc tcagtcacga actactcctc cttcccacct cgtggccccg
     1321 gaaagaacgc ccgtcttgct agctgcgccc gcatagccca gacgaacgaa gatcttccct
     1381 gcttggactc cgtgaggaaa ctactacggt gtccgagagc gcaacctacc tttcccatac
     1441 ccgagtcggg ttcacccgtt ttcggcgctc gggtgctttc ccaccaccgc ggaacgccga
     1501 agctagctca aaacgttatt ataattacgg gcacaaatca aggtaagatt tcggagtgag
     1561 tcggtctcga gcctggggga tggcatggcc aattcggtgg agctctacgt cacggcgggc
     1621 tgcccttaca gcgcctccgc ccgcgaggat ctggagtggc gcggggtgga cttcgtagag
     1681 tacgacgtcg agcaggaccg cgacgcctac gagcggatgc tcgagcttac gggcggtaac
     1741 cgcacggtcc ccgtgatagt ggaggagggt aagcccgtcc aggtaggctg gatgggccag
     1801 ggttgcttcg tgtagggggc gctttgcgaa ccaccccaaa cgatcgtcct cgtatcctgt
     1861 agcatactgc gggtccgtcg ggaggcccag tcaggagcga ggttggaaga tcggggtcgg
     1921 agcgccgccg gcagagaggc gccggagacg aaggatcggt acccgcacct cctcgcggtc
     1981 ccgacccgct ggatcgacaa cgacgtctat ggccacgtca acaacgtcgt ctactactcg
     2041 tacttcgaca cggtggtaaa cgggtatttg atctcctccg gcgccctcga catcgagaag
     2101 agctccgtca tcgggctcgt cgtcgagacg cagtgccgct acttcaagcc cataaccttc
     2161 ccagacgccg tgcacgctgg cctgcgcgta gcccgtctcg gcaagagcag cgtccggtac
     2221 gagatcggga tcttccgcaa cgacgaggag accgcagcgg cccaggggca cttcgtccac
     2281 gtctacgtcg accgcgaaaa ccgccggccc acaacgctcc caccagtgat gagggaggcg
     2341 ctggaacggg tactcctccc ggaggggtag ccgggcctac ggcgcggcga gggaggcgac
     2401 gcgcggcgcg accaccgtcg cgagctcctc gagcagcgcc tcgtcctcct gcgtgaaggc
     2461 gccgcgctcg tcgctgtcta tgtcgaactg gccgacgatc cgcccgtcct cgcggatgag
     2521 gaccacgagc tccgagcggg tcgagagcga gcaggccagg tagttgtcga gctcgcgcac
     2581 gtcatcgacg agctggttcc tgtcctgggc gaccgcggtg ccgcagaccc catcgcctac
     2641 cggtatgcgc gagtgctccg gtggctcctc cgatccggcg aagggcccga ccacgagcgt
     2701 accgtcaacg agcaggtata ttccgctcca gtcccagcgc gggtgcgcct catggacgag
     2761 ccgcacgacc tcctccagta cctcgtcgtc cggtgcgtcc gaggctacca gcgcctccac
     2821 ctcggcccgc agcttcgagc ggtcccggct gttcatgctc tgtgctctca tcggtggtct
     2881 cctcgtcgtc gcgaaaggat tcccggatat gatagcgtct gaccccactt cggctcaccg
     2941 cacgttgcgg gtgccctgtt cgtccatgta gccgaggtgt agatgatcct cgtggagctc
     3001 gagctggtcg cgctccagga tccatccctt ctgacgcccg tagcccagag cgctcgtcca
     3061 gccaggcggg cctatgatct ggtccggcct gacccgcgcg ggcatctgcg ccagtgccct
     3121 tccgatgttc aagacatccg ggtccgtggc gt
//
LOCUS       DASTUV010000221         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_291541, whole genome shotgun sequence.
ACCESSION   DASTUV010000221 DASTUV010000000
VERSION     DASTUV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291541"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1621
                     /locus_tag="VFT03_04450"
     CDS             122..1621
                     /locus_tag="VFT03_04450"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4847426.1"
                     /translation="MAQAFIHGGYTDLPGLDRMSIVDPSSGEEVDTVPSSGEAEVDQA
                     VRSACEAFPAWRDTPAAKRGEILGEAAEAVLATREELAPLLTSEQGKPLREARLEIRR
                     FVHTLEHYVGLAKNIRGGYVPDLDEDTYGLIIRRPLGVIGAIVPWNFPTTLLGNKLGP
                     ALITGNVVVAKPADTTPLTTLRIAEIMHQAGLPAGVFNVITGTGEGAGQALAEHPRVR
                     KIAFTGSTPTGKKLMAIAAGDLKRTTLELGGSDPMIVCDDANLDKAASAASVGRFFNC
                     GQACLAIKRLYVFESVADEFIEKLVGKVQKLRVGPGTAEGVMLGPLHSARQRDLLEEQ
                     VADAVDSGAEVLHGAKRPEGDGFEKGNFYEPTLLLEPARDSRVAKEEVFGPALPVWRV
                     SGMEEALELANGSIYGLGSSIWTADLNRASDAAERLEAGYTWINSPQIVYDELPFGGW
                     KQSGYGKEHGIEALDYYTETKSVVVKRAVATGDRAASQQLGGSESVSQR"
     gene            1628..3208
                     /locus_tag="VFT03_04455"
     CDS             1628..3208
                     /locus_tag="VFT03_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU4847427.1"
                     /translation="MIETPEWYNASVLVDRNLEAGRADKPAIYCGDEQVTYGELARRI
                     NRFGHALRGLGVDREDRVLLMLNDTPSFPTAFFAAMRVGAVPIPANTLLQPADYRFFV
                     ENSRARVVVCDEMHHETIQNALEGYDEEMVEIIVTNGRAEGAHVLEDLLEAGEDELDP
                     VATHRDDPAFWLYSSGSTGKPKGAVHLHHDIIYTCETCARHVLEVSEEDVCFSASKLF
                     HAYGLGNNLSFPYWAGASTVLFPGKPMPDAILGTAQRFEPTLFFSVPTLYNAMLNHEG
                     ASDYDLSSIRLCVSAAEALPAGIWRRWKEMFGLVILDGIGSTEMLHFFISNTPEEIKP
                     GSSGKPVPGYESKILDEEGYPVEPGEAGYLSVKGDSAAAYYWRNHEKTKKTMQGEWLF
                     TGDWYRMDEDGFYWYEGRSDDMIKVSGLWVSPVEVESTLGDHPAVIEVAAVGVPVDGL
                     TRVRAHIVLRQEYEPSEELVTELQNWCKERLKRYQYPHLVEFVDELPKTVTGKIQRFK
                     LREPETGVQMPRQEDELA"
     gene            3218..3400
                     /locus_tag="VFT03_04460"
     CDS             3218..3400
                     /locus_tag="VFT03_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847428.1"
                     /translation="MTRIKEACIVGAVRTLIGRHGALGFVRPDDLGALAAGLSENGAG
                     VLLEAGSREVGEAQIT"
     gene            3429..4313
                     /locus_tag="VFT03_04465"
     CDS             3429..4313
                     /locus_tag="VFT03_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4847429.1"
                     /translation="MLDGIPLIDAHVHAARLPTLKLEWREWAQNFGKDVPLDDLYDEE
                     GTMMPARFDEYMAGEGVDVAVLFCEYSPKSTGIQPVEDLVPLLAHNPARFKLMANLNP
                     HLHFPLVAELERQIELGAVGLKVHPVHGSFAPNDRMLYPAYAYCERESFPVVFHCGTS
                     VFPGSTNRYADPALIEDVARDFPDLTIVLAHGGRGWWYDAAAFITLMRPNVWIEISGL
                     PPKRLPDYYRNFDFERLAQKMIFGTDWPGVPGPRSNAQDVADLGLDLKTLEKIFYKNA
                     LNVYKRMGAGKLTAIDID"
     gene            4360..>4570
                     /locus_tag="VFT03_04470"
     CDS             4360..>4570
                     /locus_tag="VFT03_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HEU4847430.1"
                     /translation="MTAVTEEILLDRIKSGRLIEDSSQATERYIEGLKRTLIVSADTE
                     LISAPAYMNATKDFSTLPSINNYITV"
BASE COUNT          906 a         1456 c         1469 g          739 t
ORIGIN      
        1 agtgaagtcg gggtgaatac aagctgaggt gtttcccgct ggctgcccaa gcctgtatgg
       61 gattcacgct gaacggcatc ctggcgtcgc gacgtaggcc attaggtagg aggaggcctt
      121 catggcacaa gcgttcattc acggcggcta taccgacctt ccggggctcg atcggatgtc
      181 catcgtcgat ccgtcgagcg gggaggaggt ggataccgta ccctcgagtg gggaggcaga
      241 ggtggaccag gcggtcagga gcgcctgcga agccttcccc gcctggcgcg acaccccggc
      301 ggcgaagcga ggcgagattc tcggtgaggc ggcggaggcc gtcctcgcca cgagggagga
      361 gctggcgccg ctgcttacct ccgagcaggg aaagcctcta cgcgaggcgc gcctggagat
      421 tcggcgcttc gtgcacacgc tagagcacta cgtggggctt gcgaagaaca tcaggggcgg
      481 gtacgtgccc gacctcgacg aagacaccta cggcctgata atcaggcgtc cgttgggagt
      541 aataggggcg atagtcccct ggaacttccc tacgacgctt ctcggcaaca agctaggtcc
      601 cgccctgatc accggcaacg tcgtcgtggc gaagcccgcc gacaccaccc cgctcaccac
      661 cttgaggatc gcggagatca tgcaccaggc tggcctgccg gcgggcgtgt tcaacgtcat
      721 caccggcacg ggcgaggggg ccgggcaggc gctcgcggag catccccggg tccgcaagat
      781 cgcctttacg ggctctacgc caacgggcaa gaagctgatg gccatagcgg cgggcgacct
      841 caagcgcacc acgctagagc ttgggggctc ggatcccatg atcgtctgcg acgatgcaaa
      901 ccttgacaaa gccgcgagcg cagcgagcgt ggggaggttc ttcaactgcg gtcaggcgtg
      961 tctggccata aagcgtctct acgtcttcga gagcgtggcc gacgagttca tcgagaagct
     1021 cgtgggcaag gtccaaaagc tcagggttgg tccagggaca gcggaggggg taatgctcgg
     1081 gccgctgcat tctgcacggc agcgagacct cctggaggag caggtcgccg acgccgtgga
     1141 ctccggagcc gaggtgctgc acggggcgaa gaggcccgag ggcgacggct tcgagaaggg
     1201 caacttctac gagccaaccc ttctgctgga gcccgctcga gactcgcggg tggcgaagga
     1261 ggaggtcttc ggacccgcgc tacccgtctg gcgagtctcg ggcatggagg aggcgctcga
     1321 gttggcgaac ggctcgatct acgggctggg atcttcgatc tggacggccg acctcaaccg
     1381 ggcttccgac gccgccgagc gcctggaggc cgggtacacc tggatcaatt ctccccagat
     1441 cgtctacgac gagctgccct tcggcggctg gaagcagtcg ggctacggca aggagcacgg
     1501 catagaggcc ctcgactact acacggagac caagtcggtc gtggtcaaga gggcggtggc
     1561 tacaggcgat agggccgcga gccagcaact cggcggctcc gaatcggtat cccagaggtg
     1621 atgcagcatg atcgagacac cggagtggta caacgctagc gttctcgtcg accggaacct
     1681 ggaggccggg cgcgcagaca agcccgccat atactgcggc gacgagcagg tcacctacgg
     1741 ggagctcgcc cgccgcatca acaggttcgg acacgccctg cgggggttgg gcgtagaccg
     1801 ggaggacagg gtactcctga tgctcaacga tacgccgtct ttcccaacgg ctttcttcgc
     1861 cgctatgcgc gtgggcgcgg ttccgatccc ggccaacacc ctactccagc ccgcggacta
     1921 ccgcttcttc gtggagaaca gccgcgcacg cgtcgtggtc tgcgacgaga tgcaccacga
     1981 gactatccag aatgccctcg aagggtacga cgaggagatg gtggaaatca tcgtcacgaa
     2041 cggtcgagcc gaaggagctc acgtactgga ggacctgctg gaggcggggg aggacgagct
     2101 cgacccggtc gcgacgcaca gggacgatcc ggcgttctgg ctctacagct cgggctctac
     2161 cggaaagccc aagggcgcgg tccaccttca ccacgacatc atctatacct gcgagacctg
     2221 cgcccggcac gtgctggagg tctccgagga ggacgtatgc ttctcggcct caaagctctt
     2281 ccacgcttac ggcctgggta acaacctctc cttcccgtac tgggcagggg cctcgacggt
     2341 gctcttcccc ggcaaaccca tgccagacgc gatcctgggc accgcccagc gcttcgagcc
     2401 gacactcttt ttctcggtgc cgacactcta caatgccatg ctcaatcacg agggcgccag
     2461 cgactacgac ctgtcctcca tccgcctgtg cgtctcggcg gcggaggccc tccccgccgg
     2521 catctggcgc cgctggaaag agatgttcgg cctggttatc ctcgacggca tcggctcgac
     2581 ggagatgctg cacttcttca tctccaacac gccagaggag ataaagcccg gctccagcgg
     2641 caagccagtg cccggctacg agtccaagat cctggacgag gaaggatatc ccgtcgagcc
     2701 aggcgaggcg ggatacctct cggtcaaggg tgattccgcc gcggcgtact actggcgcaa
     2761 ccacgagaag acgaagaaga ccatgcaggg cgagtggctc ttcaccggtg actggtacag
     2821 gatggacgag gacggcttct actggtacga gggtcgctcg gacgacatga tcaaggtcag
     2881 cggcctgtgg gtctcccccg tcgaggtcga gagcaccctc ggagatcatc cggcggtgat
     2941 agaggtcgcc gccgtcggcg tcccggtaga cggattgacg cgggtgaggg ctcacatcgt
     3001 cctgcgccag gaatacgagc catccgaaga gctggtcacc gaactacaga actggtgcaa
     3061 ggagcgcctc aagcgctacc agtacccgca tctggtggag ttcgtggacg agcttccgaa
     3121 gacggtgacg ggcaagatcc agcgcttcaa gctgcgcgag ccggagacag gggtccagat
     3181 gccccggcaa gaggacgagc tggcctagag gaggatcgtg acacggatca aggaagcctg
     3241 tatcgtcggc gcggtccgca ccctcatagg ccgccacggc gctcttggct tcgtgcgccc
     3301 cgacgatctg ggcgctctgg cggcgggact gagcgagaac ggcgcagggg tgctgctgga
     3361 ggccggctcc cgagaggtcg gcgaggctca gatcacgtga tcccggctca accgacgaac
     3421 ggaaaaccgt gctagacggc atccccctca tagacgccca cgtccacgcg gcgcgattgc
     3481 ccaccctcaa gctcgagtgg cgcgagtggg cgcagaactt cggcaaggac gtgcccctcg
     3541 acgacctcta cgacgaagag gggaccatga tgcccgcccg cttcgacgaa tatatggccg
     3601 gggagggcgt agacgtcgcc gtcctcttct gcgagtacag cccgaagtcc accggcatac
     3661 agcccgtgga agatctagtc cctctcctcg ctcataaccc tgcccgcttc aagctgatgg
     3721 ccaacctcaa cccgcacctg cacttcccgc tggtcgcaga gctggagcgc cagatcgagc
     3781 tcggcgccgt cggcctgaaa gtccatcccg tccacggctc tttcgccccc aacgaccgga
     3841 tgctctaccc agcctacgcc tactgcgaaa gagagagctt ccccgtcgtc ttccactgcg
     3901 gcacgagcgt cttcccgggc tcgacgaacc gctacgccga cccggccctc atcgaggacg
     3961 tcgcccgcga ctttcccgac ctcaccatag tcctcgccca cggcgggcgc ggctggtggt
     4021 acgacgccgc agcctttatc acgctcatgc gccccaacgt ctggatcgag atctccggcc
     4081 tcccccccaa gaggctccca gactactaca ggaacttcga cttcgagcgg ctggcccaga
     4141 agatgatctt cggcaccgac tggcccggcg tgccaggccc gagatccaac gcccaggacg
     4201 tcgccgacct cggcctcgat ctcaagacac tggagaagat cttctacaaa aacgccctca
     4261 acgtttacaa aagaatgggg gcgggtaaac tgacagcgat tgatattgac tgaccggaca
     4321 gttattatgc tgcggccggg tcagacggag gaggaaggca tgacggcggt aactgaggag
     4381 atactcctgg atcgcatcaa gagcgggagg ctcatcgagg actcttcgca ggcgacggag
     4441 aggtacatcg agggtttgaa gcgtaccctg atagtctcgg ctgacaccga gctgataagc
     4501 gctccggcgt acatgaacgc gacgaaggac ttctccacgc tccccagcat caacaactac
     4561 atcaccgtca
//
LOCUS       DASTUV010000222         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1327954, whole genome shotgun sequence.
ACCESSION   DASTUV010000222 DASTUV010000000
VERSION     DASTUV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327954"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1514
                     /locus_tag="VFT03_04475"
     CDS             180..1514
                     /locus_tag="VFT03_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU4847431.1"
                     /translation="MNVIVVILDSLRKDHVRIYGNDRVRTPSLDALAGQSLRFTRAYP
                     ESIPTLCARRAIHTGLRTWPFRNWDPPEGEDFMPAGWQQIPEEQTTISEILEEQSFNT
                     VLLSDTHHMFKASMNFQRGFKVFDWIRGQERDGYRPIMGVSQEKVDQMTVPGNSRSME
                     DKVRQYLANTKDRKSEEDWFAPRVFSMASDFLRTADKGQPFFLVVDCFDPHEPWDPPD
                     RYVSLYDDSYDGPEPTVPEYSDAGWIDERELERMRALYAGEVTMADRWFGRFVDTLDS
                     SDFAKETLLVVLSDHGVALGEHDATGKPFWALYPELTDIPFMIRHPEGTRGGETSDYY
                     ASTHDVAPTILGFLDMEPRSPMGGQDLSVIFGGGQPSERNHFTLGYDEYVWARDDRYV
                     LLSRNDRAQTKLYDLSADPDMNTDIADANPDVARRMFDDYVIGDAGGPLPRY"
     gene            1516..>3672
                     /locus_tag="VFT03_04480"
     CDS             1516..>3672
                     /locus_tag="VFT03_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEU4847432.1"
                     /translation="MALRAFSFLLRWTGLLCVPGGVLWALSPLGVHISDVRFHTPNVF
                     WKLFPSAPLLLLVGLLGLHFLVSGRSGWLEKAGFFMALLGLVMILVGDVGEFWLGLDD
                     RYIMAAPAYRALRLGLLAFGVGSILFGVATGRDRTLPVWGALPFAITSLCGLISFSRD
                     LGQFGTALWILFGLGWAWLGFTLLVDGLARFWQKRQAAPRSGDGGLQASAPSEGPPEA
                     AGDAPSGVPEVAATDAIGGDRDDTYVARVARGAGISTAGQGAGRVLGYLTQVMIARLY
                     GPAAFGFYSLGVAAVNGAQILSRFGMENGVVRYVAHHQAREDTPRVRGTIIQAIGVAT
                     ALSVVISAVMFFGAGFAAEWYYNKPFMETVLKAFSLTLPFFTFMMMVLWATQGFQTVT
                     YASYVQQMIRPALFLALVPVFYLLGAGIIGTVAAYGVSMLLGSLLAVYYLRKLFPPLF
                     DNKVPARFETKELFAVSVPMSITAGAQYLNTWSAVWVMGAFAAAGPVGIFTAAARTAT
                     LSTIVRFAFSGIFSPIISSFYARDEIEDLGRLYKDVSRWIFTGAFAIFLPILLLSREI
                     LAIFGPAFTSGWTALIIVAAAQLYSSSVGPTPRMLAMTGNQNVAMVATSVAALVGVAV
                     SFALVPSLGMLGAALGMASAITTENTGTMLAVKRRLGFWPYSWAWLKPLAAGILAAAA
                     AFLAELVLPLPGFLVTIAVVGAVFGAGYLILLLLLGL"
BASE COUNT          592 a         1229 c         1166 g          685 t
ORIGIN      
        1 aggggctgag gaagactctg gactcgttcg tggggcgctc gaaccggccc gaagcctcgc
       61 ccacggggcg ttgaggggcg ctacccgcgg attattccat tagaatatgg cgctgcgcgg
      121 gcggcgacga taggtcccgc cggcccgaag gtaggttacc ttccgagtga gaggacgcaa
      181 tgaacgtcat agtggtgatc ctggacagcc tgaggaagga ccacgtccgg atctacggca
      241 acgacagggt caggaccccg agcctcgacg cgctggccgg acagagcctg cgcttcaccc
      301 gggcataccc cgagtccatc ccgaccctct gcgcgcggcg cgccatacac acaggcctca
      361 ggacctggcc tttcaggaac tgggacccgc ccgaagggga ggacttcatg ccggcgggct
      421 ggcagcagat ccccgaggag cagaccacca tctccgagat cctcgaagaa cagagtttca
      481 acaccgtcct gctctcggat acccaccaca tgttcaaggc ttcgatgaac ttccagcggg
      541 ggttcaaggt gttcgactgg atcaggggcc aggagcggga cggttaccgc ccgatcatgg
      601 gggtctcgca ggaaaaggtg gatcagatga ccgtccccgg caactcccgg agcatggagg
      661 acaaggtccg ccagtacctg gccaacacca aggaccggaa atctgaggag gactggttcg
      721 ctcctagagt gttcagcatg gcctcagact ttcttaggac cgccgacaag ggccagcctt
      781 tcttcctggt tgtcgactgc ttcgatccgc acgagccgtg ggatccgccg gacaggtacg
      841 tctccctcta cgacgatagc tacgacggcc ccgagccgac cgtgccggag tacagcgacg
      901 ccggctggat cgacgagcgg gagctcgagc ggatgcgcgc cctctacgcc ggtgaggtca
      961 ccatggccga ccgctggttc ggccgctttg tcgacacgct cgactcctcg gacttcgcga
     1021 aggagaccct gctcgtggtc ctctccgacc acggcgtggc tctcggcgaa cacgacgcca
     1081 caggcaaacc cttctgggcg ctctaccccg agctcaccga tatcccgttc atgatccgtc
     1141 atccggaggg cacgagaggt ggagagacca gcgactacta tgcctccacc cacgacgtgg
     1201 cacccacgat cctgggcttt ctcgacatgg agccgagatc cccgatgggc ggccaggacc
     1261 tctccgtgat cttcggcggt ggccagccgt ccgagcggaa ccacttcact ttaggctacg
     1321 acgagtacgt gtgggcgcgc gatgaccgct acgtcttgtt gagccgcaac gaccgagcgc
     1381 agaccaagct atacgacctg agcgcggacc ccgacatgaa cacggacata gctgacgcca
     1441 acccagacgt ggccaggcgg atgttcgatg actacgtcat cggcgacgcg ggcggcccgc
     1501 tgccgaggta ttgagttggc gctcagggcg ttctcgtttc tccttcggtg gaccggactg
     1561 ctgtgcgtgc caggaggcgt gctgtgggcg ctatctccct tgggcgtcca tatctccgat
     1621 gtgaggttcc acaccccgaa cgtcttctgg aagctcttcc cctcggcgcc gctgctgttg
     1681 ctggtcggcc tcctcgggtt gcacttcctc gtctccgggc gctccgggtg gctcgagaag
     1741 gcgggtttct tcatggcgct tctcgggctg gtcatgatcc tggtcggcga cgttggcgag
     1801 ttctggctcg ggctggacga ccgctacatc atggcggctc ccgcctaccg cgccctccgg
     1861 ctcgggctgc tcgcatttgg tgtgggctcc atacttttcg gcgtcgccac aggaagggac
     1921 aggacgctgc cggtctgggg cgcgttgccg ttcgccatca cgtcgctctg cgggctaatc
     1981 tccttctcca gggacctggg acagtttggg acggcgctct ggatcctgtt cggcctcggg
     2041 tgggcctggc tgggcttcac cctcctagta gatgggctcg cgcgcttctg gcagaagcgg
     2101 caggcggcgc cgaggtcagg agatggagga ctccaggcga gcgctccctc tgaagggccg
     2161 ccagaggcgg cgggcgacgc gccctccggc gtaccggagg tggccgcgac cgacgctata
     2221 ggcggcgaca gggacgacac ctacgtggcc cgcgtagcac ggggagccgg catcagcacc
     2281 gccgggcagg gagcggggcg ggtgctcggg tacctgacgc aggtcatgat cgcccggctg
     2341 tatggcccgg cggcgttcgg gttctactct ctgggtgtcg ccgccgtgaa cggggcccag
     2401 atactcagca ggttcgggat ggagaacggc gtggtgcgct acgtagctca ccaccaggcc
     2461 agggaggaca ccccgcgggt gcggggcacc ataatccagg ccatcggggt ggcgaccgct
     2521 ctgagcgtgg tcatctccgc cgtgatgttc ttcggggcgg ggttcgcagc ggagtggtac
     2581 tacaacaagc ccttcatgga gaccgtcctg aaggccttct cccttacgct gcccttcttc
     2641 actttcatga tgatggtcct gtgggccacc cagggcttcc agaccgttac ctacgccagc
     2701 tacgtgcagc agatgatacg ccccgccctg ttcctggcgc tcgtccccgt cttctacctg
     2761 ctaggggccg ggatcatcgg taccgtggcc gcctacggcg tctcgatgct cctcggctcg
     2821 ctcctcgccg tgtactacct aaggaagctc ttcccgccgc tgttcgacaa caaggtgccg
     2881 gccaggttcg agacaaaaga gctcttcgcc gtctcggtgc cgatgagcat cacagcaggg
     2941 gcgcagtacc tcaacacctg gtcggcggtc tgggtcatgg gcgccttcgc cgccgcgggg
     3001 ccggtaggca tcttcaccgc agcagcccgc accgcaacgc tctcgaccat agtgcgcttc
     3061 gccttcagcg gtatcttctc gccgataatc tcgagcttct acgccagaga cgagatagag
     3121 gacctcggaa gactttacaa ggacgtgtcg cgctggatct tcaccggcgc tttcgccata
     3181 ttcctgccca tattgctcct ctccagggag atactggcta tcttcggccc cgccttcacg
     3241 tccgggtgga cggccctgat catcgtcgcc gccgcccagc tctatagctc ctcggtcggt
     3301 cctacgccgc gcatgctcgc gatgaccggt aaccagaacg ttgccatggt cgccacatcc
     3361 gtcgccgccc tcgtgggcgt ggccgtgagc ttcgccctcg tcccgagcct cgggatgctc
     3421 ggcgcggccc tcggcatggc ctccgctata actaccgaga acacggggac aatgctcgcc
     3481 gtgaagcggc gcctcggttt ctggccatac agctgggcct ggctcaagcc cctcgccgcc
     3541 ggcatcctcg ccgccgccgc ggccttcctc gccgagctgg tgctgcccct cccaggcttc
     3601 ctggtgacca tcgccgtcgt aggcgcagtc ttcggcgcgg ggtacctgat cctgctcctc
     3661 ctcctcggtc tc
//
LOCUS       DASTUV010000223        10669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_875925, whole genome shotgun sequence.
ACCESSION   DASTUV010000223 DASTUV010000000
VERSION     DASTUV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875925"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..916
                     /gene="uppP"
                     /locus_tag="VFT03_04485"
     CDS             92..916
                     /gene="uppP"
                     /locus_tag="VFT03_04485"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEU4847433.1"
                     /translation="MLEVLEAILLGVVQGLTEFLPVSSSGHLLLGQYFLGLDQDRFGL
                     AFDVALHLGTLVAVVSFFWRDLVRMAGAFTRSLTPGGRDFSDPDQRLAYLILASTIPA
                     ALIGFFLEDFFETSVRNPWVVVFNLVLVGVLFIVGEAVGSKTRGADKLRFSEAIGIGL
                     AQAAALVPGVSRSGATITLGLFLGLRREEAARFSFLMSVPIIAGAGALQLTQAVSEGM
                     GRSEALMFAAGFVSSAVVGYLAIKFFLRYVVHHSLRAFAYYRFGLAAVVAVILLIP"
     gene            1091..2251
                     /gene="pheA"
                     /locus_tag="VFT03_04490"
     CDS             1091..2251
                     /gene="pheA"
                     /locus_tag="VFT03_04490"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU4847434.1"
                     /translation="MFPSDASNENTAESVVKQDLRSQKRTKDEMEALRAGIDEVDEKI
                     VRLLDQRARLARRIGEIKQENGLEAYAPARERAVLDRVTSLGAGDYPMRGLQAVFREI
                     ISGSISLEARLKVAYLGPESTFTHEAALRSFGASVELEPQTTVAEVFTRVERGEAEHG
                     IVPVENSMEGAVTHTLDELMNSPLKICGEVYLPVSQNLISSEPALDRVRVVCSHPMAL
                     SQAAAWLRHHLPGARLEEVESTAQAARRAAGEPGVAAVGSALAAEAHHLNVLARSIQD
                     ARANATRFIVLGRAWAGRTGTDKTSVVFSVKDRPGVLRDALAAFAEEGINLTRIESRP
                     SRKRAWTYVFFADFVGHPEDERVQRALEALEEHCPYVSLIGAYPAIPPEMIY"
     gene            2258..2482
                     /locus_tag="VFT03_04495"
     CDS             2258..2482
                     /locus_tag="VFT03_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU4847435.1"
                     /translation="MDFKAAAREVLKEMGHPLHYSDITEIALESGYLRSVGRTPQNTM
                     RARLSVDVRDNPESPFFQSAPGVYGLKGSL"
     gene            2543..3565
                     /locus_tag="VFT03_04500"
     CDS             2543..3565
                     /locus_tag="VFT03_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847436.1"
                     /translation="MVEVANRAVEAAWKIRNLEDYDRRLERAAARADELAREHETAAL
                     VIYEAALATSGRPREEVRELVRSFRDTFEDEEEPLTIPRTSGIMNVEDDSWFFGDEHL
                     RVLTGRLLGQFQHRVAQYNYIDEREVLRRWADGYDTRLFIRRRIFETEPVVGVVERFD
                     LRQVQYLRLIAGGNTLVPSEDAARALEALGYEAGADDYAVLARAEELALYLELPAPVV
                     GEMLEDLGREGYTDFPEPPREAVRPEEPAEGAEEDGVASEGGEVSGGAAAEASEEKPS
                     PGERAARRKVREDAGKGEEPTRVQDPQARDTPGVEQESGSKKDPDAPETGRGGASGSS
                     DRKADS"
     gene            3682..4317
                     /locus_tag="VFT03_04505"
     CDS             3682..4317
                     /locus_tag="VFT03_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU4847437.1"
                     /translation="MADTKKRIALKPHMEEIREWVEDGRSDDWIADALGTSASSVQSF
                     RSRNDIYRRERGPREPESVFEGVLDHGERDGWGLWLDPAIAEDPIWKEHWAGVEAVTV
                     KVAEDSIVLEADTEGTAAASTGGIRPVVQVPLDGQAENSDGPPRERGRVKWFDAEKGY
                     GFLVRPTGEDLFVHHSEVQGDPSSLSPNDEVEYEVGQNDRGPNARRVQPTN"
     gene            complement(4328..4981)
                     /locus_tag="VFT03_04510"
     CDS             complement(4328..4981)
                     /locus_tag="VFT03_04510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4847438.1"
                     /translation="MPEQEIFLIRHGQSTANASGVWQGQLEFPLSEEGRLQSRLAANA
                     ITGQPFDAIYSSPLGRAFETAEIIAREAGFTGEIVPIAGLMERHGGSLEGTTHAERET
                     RNPELTRKLLGLPEEERWSLVGAETDEEVLARFEEAISDIRARHDAGARLVVVSHGGA
                     MRAFLRDHFGSGVLPGTVRAANASITRIGWAKNGEPRLLELASTRHLSSTPRSSVTE"
     gene            5075..5872
                     /locus_tag="VFT03_04515"
     CDS             5075..5872
                     /locus_tag="VFT03_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847439.1"
                     /translation="MQWSNGGDTPEEVPVGRIQLERDAPRPATILKVATELEERGAEI
                     RELFKEVRAPWGEVALPIHLVRGGRDFFIEVETGPWEPGTTEQAVNKAAVLRDSDYAG
                     AGLEILSAYPVPREVGFFFGRSPAALLQLALVRVTADRPEEAAELFRETASVQWSVDL
                     DYEQEYLPLVEELVMAALDEDEEPPILDALVVGLGCFVGEIIRRNANPPGAWRPDESW
                     GEGPVIEAGWLVLDPIGKSRAFLLEGADDSIAFYADYVLEQLTTGPE"
     gene            complement(5892..6143)
                     /locus_tag="VFT03_04520"
     CDS             complement(5892..6143)
                     /locus_tag="VFT03_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEU4847440.1"
                     /translation="MDATLCKTAEIKLYTGAYCPYCRIVQRELERLGLDYEVVDADVD
                     GRDEVIRLSGQRAIPILTIDDEVLVDSSHIVRELRRRCA"
     gene            complement(6247..6714)
                     /locus_tag="VFT03_04525"
     CDS             complement(6247..6714)
                     /locus_tag="VFT03_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU4847441.1"
                     /translation="MSPDPESRLRELGLELPAVPEPAGAYVSANRTASLIFTAGQLPF
                     ENGELRITGKVGDAVSADEAYEAARLCALNGLAAAAAEAGSLNGIGRVVKVTGFVASA
                     PGFNAQPRVVNGASDLIGELFGEAGLHARSAVGVAELPLDSPVEVELVVELEA"
     gene            complement(6715..7209)
                     /locus_tag="VFT03_04530"
     CDS             complement(6715..7209)
                     /locus_tag="VFT03_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU4847442.1"
                     /translation="MDSEPHDRGSILGAMRNLTHGVYVLGTRRGRQSNAMTASWVMHA
                     SERPPCVAVAVREDRYSHDLILESSTFALSALRNDQVDVATHFSDTSGEYHDKLQGVP
                     YGLTPGGSPFLLDCLAYLDCRVMDTLRSGDHTVIVGEVTAGETLADGYPLIYDPTEYE
                     EALR"
     gene            7303..8292
                     /locus_tag="VFT03_04535"
     CDS             7303..8292
                     /locus_tag="VFT03_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU4847443.1"
                     /translation="MELSASLVWFGVLQTLIYLLFIRTIDLYEREPLRYVLSVFIWGF
                     AVATTISLVFNTIAQITFDALVSRQEANFLTAVFVAPVVEESSKGLALFIIFVIAYLA
                     SRRRHLIEFAGVMDGIVYGSAVGFGFALAEDILYGLQFGAETFLVRRILGGFGHAAFT
                     SLTGIGIGLIPFVNNRALRVLPPLLGLLGAILLHATFNFLATTMGPVAYLFMFVVVLL
                     YVMLIIVWLTIERRWIRDELRGEVDKGIISPEEYAVLPTAFRRTGYYVRLILRGHPAE
                     WSSARKVHAAAVDLAFAKRLARRWPTPTRQQRVEALRLKILDLKAQAAIRVRT"
     gene            8316..8888
                     /locus_tag="VFT03_04540"
     CDS             8316..8888
                     /locus_tag="VFT03_04540"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU4847444.1"
                     /translation="MILAGGKSRRMGRDKLLLEVGGEPLLNRVHDVLARRCAEVILVG
                     GGPARLEGVRQVQDERIGGLGPLAGIEAGLAAAEYQLVFVAAADLPFLPAELVGFALE
                     RLEERGALAVVPRHGGTAHPLCAAYDRRILPRVRSALDGGVRSVREFLDSLDRVDYVE
                     RELRRFGNPELFLMNVNSPEDLDRARAGLH"
     gene            8885..9481
                     /locus_tag="VFT03_04545"
     CDS             8885..9481
                     /locus_tag="VFT03_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU4847445.1"
                     /translation="MKPPSSRGVLEWIRTLVDVYRGGAPPPGDAPAEIQVAVVLGTEV
                     LSGGRPSSTLAARVRHAAGLYERGRVRLIIATGGLGKHPPAEADVMGRVLGEEGVPDH
                     AVMLEDRALNTWDSARFVTGMAKRLGVSDVLVVTDPLHCVRTVAAFERAGLGAWAEPV
                     YGSPMWRGSWARLGQFVRELGALVWYRIRYGVGTRSRQ"
     gene            9436..10326
                     /locus_tag="VFT03_04550"
     CDS             9436..10326
                     /locus_tag="VFT03_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4847446.1"
                     /translation="MVQDKVRGRYALPAVAVGVLAVSAAAIFIRLAEAPAIAVAFWRC
                     ALGAALLLPPALVRRDRFPRGRDLWVGIASGIALGAHFGFWISSLDHTSVAASVVLVS
                     TQPVFVAILAYLIFGERTSPLSFAGILAALVGTAIIAGDDTVGSAALLGNALALAGAV
                     TVAVYVLIGRYSRTGGIGVLPYSVVVYSAAALALLPVALVLDVRLWGYSGETWFWLWA
                     ITLGPQLMGHTVFNWALRYVEASVISGTILAEPVISSLLAWLILSETPGLPTILGGCI
                     VLIGLFLLLRGRKVPAEPIG"
BASE COUNT         1756 a         3337 c         3667 g         1909 t
ORIGIN      
        1 gaccctggct gacaagcgga ggccgcaggc cgaagcgtgc tgacaagccg ccgaccgaaa
       61 ggaggcgtgc taagaaggga ggcgtactta agtgcttgag gtcctggagg ctatactgct
      121 cggcgtggtg caggggctca cggagttctt gcccgtctcc agctcggggc acctgcttct
      181 cgggcagtac ttcctggggc tggaccagga ccgcttcggg cttgccttcg acgtcgcgct
      241 gcacctcggc acgctcgtcg ccgtggtctc ctttttctgg cgtgatctgg ttcgtatggc
      301 cggggcgttt acgcgctcgc tcacgcccgg cgggagggac ttctccgatc cggaccagcg
      361 gctggcgtat ctcattctgg cctcgaccat tcctgcggcc ctgatcgggt tcttcctgga
      421 ggacttcttc gagacgtccg tgcgcaaccc ttgggttgtg gtcttcaatc tggtgctggt
      481 cggtgtgctg ttcatcgttg gtgaggccgt gggaagcaag acccgagggg cagacaagct
      541 gaggttctcg gaggctatag gtatcgggct cgcgcaggcg gcggcgctcg tccccggagt
      601 ctcgcgctcc ggggcaacga tcacgctcgg gctcttcctg ggtctgcggc gcgaggaggc
      661 ggcccgcttc tcgttcttga tgagcgtccc catcatcgcg ggggccggcg cgctgcagct
      721 cacccaggcg gtgtcggagg ggatgggacg ttccgaggcg ctcatgttcg cggcgggctt
      781 cgtctcttct gctgtggtcg ggtacctggc gataaagttc ttcctgcgct acgtggtcca
      841 ccactctctg cgtgccttcg cttactaccg cttcgggctc gccgccgtcg ttgcggtgat
      901 attgctgatc ccgtgactgc gcaatttttt tgacatccac cgcgtacttt tggtactttt
      961 acactgttat gcgggttgcg gtctcggctt ttgggcgatt taccggggag gggcgtgccc
     1021 atcccggatg cgcggccacg ctcctcctac gctgaacgtc gcgtagactc ccaccgaaag
     1081 gccgtactag atgtttccct ccgacgcatc gaacgagaac acagccgaga gtgtggtgaa
     1141 gcaggatctc cgctcccaga aaaggaccaa ggacgagatg gaggcgctgc gagccgggat
     1201 agacgaggtg gacgagaaga tcgtacgtct gctagatcag cgggctcggt tggcgcgcag
     1261 gatcggggag atcaaacagg agaacgggct cgaggcctat gcgccagcca gggagcgggc
     1321 ggtgctcgac cgcgtgacgt ccctcggggc aggcgactac ccgatgcgcg ggctccaggc
     1381 ggtcttccgc gagatcatct ccggctccat ctcgctcgag gcacgcctga aggtcgccta
     1441 cctcggtccc gagtccactt ttacccacga ggcggcgctg cgctcgttcg gggcgagcgt
     1501 ggagctggag ccgcagacga cggttgcaga ggtcttcacc cgcgtggagc gcggcgaggc
     1561 agagcacggc atcgtcccgg tcgagaactc gatggagggc gccgttaccc acaccctgga
     1621 cgagctgatg aacagccccc tgaaaatctg cggcgaggtc tacctgcccg tctcgcagaa
     1681 cctcatctca tccgagcctg ccctggatcg cgtacgagtt gtctgttcgc atccgatggc
     1741 cctctcccag gcggcggcct ggctgcgcca ccatctcccc ggcgcgcgcc tggaggaggt
     1801 cgagtcgacc gcgcaggcgg cccgccgcgc cgccggagag ccgggagtgg ccgcggtcgg
     1861 tagcgctctg gcagccgaag cgcaccacct gaacgtgcta gcccgcagca tccaggacgc
     1921 ccgcgccaac gccacgcgct ttatcgtgct cggccgcgcc tgggccggga ggacgggtac
     1981 ggacaagacg agcgtcgtat tctctgtcaa ggaccgcccc ggcgtgctgc gcgacgcgct
     2041 cgcggccttt gcggaggaag gtatcaacct gacgcgcata gagagccgcc cgagccgcaa
     2101 gcgggcctgg acctatgtgt tcttcgcgga cttcgttggg caccccgagg acgagcgggt
     2161 gcagcgggcg ctcgaggcgc tcgaggagca ttgcccttac gtgagcctca tcggagcata
     2221 ccccgcgatt cctcctgaga tgatctacta gagaaggatg gacttcaagg ccgccgcgcg
     2281 ggaggttcta aaggagatgg gccatccgct ccactactcc gacataaccg agatcgcgct
     2341 cgagagcggc tacctgagga gcgtggggcg tacgccacag aacacgatga gagcccggct
     2401 ctccgtggac gtccgggaca accccgagag cccgttcttc cagtctgctc ctggcgtcta
     2461 cggcctcaaa ggttcgcttt gagcgccccg gagatgctgt atattggacg ttcggagggt
     2521 caaaactcat cgaggagaga ctatggttga ggtagcaaac agggccgtcg aagccgcctg
     2581 gaagataagg aacctggagg attacgacag gaggctcgag cgggccgccg cgagggcaga
     2641 cgagctcgcc agagagcacg agacggctgc gctcgtgatc tatgaggcgg ccctggctac
     2701 gagcgggcgt ccgcgcgagg aggtgcgcga gctggtacgc agcttccgcg acaccttcga
     2761 ggacgaggaa gagccgctta cgatcccgcg tactagcggc atcatgaacg tcgaggacga
     2821 ttcgtggttc ttcggcgacg agcacttgcg ggtactgacg gggcgcctgc tcggccagtt
     2881 ccagcacaga gtggcccagt acaactatat cgacgagcgc gaggtcctga ggcgctgggc
     2941 cgatggctac gacacgcggc tcttcatccg gcggcgcatc ttcgagaccg agccggtcgt
     3001 aggggtggtt gagcgttttg atctccggca ggtccagtac ctgcgcctga tcgccggagg
     3061 gaacaccctc gttccctccg aggacgccgc acgagccctc gaggccctcg gctacgaagc
     3121 tggcgcggac gactacgcgg tcctggcccg tgccgaggag ctcgcgctct acctcgagct
     3181 tccggcccct gtggtggggg agatgctcga agatctcggt cgcgagggct acacggactt
     3241 ccccgagccg ccgcgcgaag cggtacggcc cgaagagccg gcggaaggcg cagaagagga
     3301 tggcgtcgca tcggagggcg gagaagtgag cggaggcgcc gcagctgaag cgagcgagga
     3361 gaagccgagc cctggggaga gggcagcccg ccgcaaagtc cgtgaggacg ccggcaaggg
     3421 cgaggaaccc accagggtgc aggatccgca ggccagggac actccagggg tcgagcagga
     3481 atccggtagc aagaaagacc ccgacgcacc cgagacggga agaggcgggg cgtcgggctc
     3541 ctcggatcgg aaggccgact cctgaattag tcgccaagat gttagaatgc gcatcaacat
     3601 cggtgatagg tcgtgaatcg aacgcttaac acatcgcatc aagacgaata tccgagacgt
     3661 taataaggag cgggcggcag catggcagat accaaaaagc gtatagcgtt aaaacctcac
     3721 atggaggaga tcagggagtg ggtggaggac ggcaggtcgg acgactggat cgccgacgcc
     3781 cttggtacaa gcgcctcgtc ggtgcagtcg ttccgctcgc gcaacgacat ctaccgccgc
     3841 gagcgaggtc cccgcgagcc agagagcgtc ttcgagggcg tcctcgacca cggggaacgc
     3901 gacggctggg ggctgtggct tgacccggcc atcgccgaag accccatctg gaaagagcat
     3961 tgggctggcg tggaggcggt gaccgtcaag gtggccgagg actcgatagt gctcgaggcc
     4021 gacacggagg gaaccgcggc ggcatccacc ggtgggatca ggccggtcgt ccaggttccc
     4081 ctcgacggcc aggcggaaaa ctccgacggg ccgccacgag agcgcgggcg cgtcaaatgg
     4141 ttcgacgcgg agaagggcta cgggttcctc gtccggccga cgggcgaaga cctcttcgtc
     4201 caccactcgg aggtccaggg tgacccctcg tctctctcgc ccaacgatga ggtggagtac
     4261 gaggtagggc agaacgaccg tggtccgaac gcgcgtcgcg tccaacccac gaactaaccc
     4321 cgactcctca ctccgttacg gaggatcgcg gggtggaaga gaggtgccgc gtagaagcga
     4381 gctccagcag ccggggctcg ccgttctttg cccatccaat gcgcgttatc gaggcgttcg
     4441 ccgcccgcac ggtgccgggc aataccccgg acccgaagtg gtccctcaag aatgcccgca
     4501 ttgcgccgcc gtgggagacg accacaaggc gcgcacccgc atcgtggcgg gcccgaatgt
     4561 cggagatcgc ctcctcgaac cgcgccagga cctcctcgtc tgtctcggcc cccaccagcg
     4621 accagcgctc ctcctccggc aacccgagca gcttcctcgt tagctcgggg ttgcgcgtct
     4681 cccgctccgc gtgagtggtc ccttcgagcg agcccccgtg ccgctccatt aggcccgcta
     4741 tgggaacgat ttctcccgtg aaacctgcct cgcgggcgat tatctcggcg gtctcgaaag
     4801 ccctgcccag cgggctggag tagatggcgt cgaacggctg gccggtaatc gcattcgccg
     4861 cgaggcgcga ctgcagacga ccttcctcgg agagaggaaa ctccaactgc ccttgccaga
     4921 cgccgctagc gtttgccgtc gactggccat ggcggatcag gaaaatctct tgttcaggca
     4981 tggcggtaag cttagtatat cgctcccgct ttcggcaact tcctccggtt gctagaatct
     5041 gcgggttcga ccgcgacagg gaaggctggc ccggatgcag tggagcaacg ggggcgacac
     5101 gccagaggag gttcccgtcg gacgcatcca actggagcgc gatgcgccgc gtccggcgac
     5161 catcctcaag gtagccaccg agctggagga gcgcggcgcc gagatacgcg agctgttcaa
     5221 ggaggtccgg gctccctggg gggaggtggc gttgccgata cacctggtgc gcggcgggcg
     5281 ggacttcttc atcgaggtcg agacaggtcc ctgggagccc ggaacgaccg aacaggcggt
     5341 aaacaaggcg gccgttctgc gggactcgga ctacgcgggc gctgggctgg agatactgag
     5401 cgcttacccc gtaccgcggg aggtcgggtt tttcttcggc cggtcgcccg ccgccctgct
     5461 ccagctagcc ctcgtccgcg tgaccgctga caggcccgag gaagcggccg agctgttccg
     5521 ggaaaccgcg agcgtccagt ggagtgtgga cctcgactac gagcaggagt atctgccgct
     5581 ggtcgaggag ctggtgatgg cagctctgga cgaggacgag gaaccgccga tcctcgacgc
     5641 tctcgtcgta gggttgggct gcttcgtggg ggagatcatc cgccgcaacg ccaacccccc
     5701 aggcgcctgg cgccccgacg aaagttgggg cgagggcccg gtgatcgaag ccggctggct
     5761 ggtcctggac ccaataggca agtcacgcgc tttcctcctc gaaggcgcgg acgactcgat
     5821 agccttctac gccgactacg ttctcgagca gctcacaacc ggcccggaat agcaggcacc
     5881 tgtgtaagtg ctcaagcgca gcggcggcgc agctcgcgga caatgtgaga ggagtccacg
     5941 agaacctcgt cgtctatggt caggatcggg atcgctcgct gcccggagag gcgtatcacc
     6001 tcatcccgcc catcaacgtc ggcgtccaca acctcgtaat ccaagcccaa cctctcgagc
     6061 tctctctgta ctatccgaca gtatggacag taagcacccg tgtatagctt tatctccgcg
     6121 gtcttgcaca gcgtcgcatc catttttcat tccctccacg ttgtacccga cgattattat
     6181 atccgatcaa acagattgta aaccttgacg ggcggcgttc aggaggacga agatccgtgg
     6241 tgtgctctag gcttcgagct cgactacgag ctccacctcg actggcgagt cgagcggcaa
     6301 ctctgcgacg cccacggcgg agcgagcgtg caagccggcc tctccgaata gctcgcctat
     6361 aagatccgag gctccgttga ctacacgcgg ctgggcgttg aagccggggg ccgaggcgac
     6421 gaagcctgtc acctttacga cgcggcctat cccgttcagg cttccggcct cggcagcggc
     6481 ggcggcgagg ccgtttagcg cgcagaggcg ggcggcttcg taggcttcgt cggccgaaac
     6541 tgcgtcgccg accttacccg ttatgcggag ctccccgttc tcgaagggga gttggcctgc
     6601 ggtgaagatg aggctcgcgg ttcggtttgc ggagacgtag gcgccggccg gctcgggtac
     6661 ggcgggcagc tcgaggccga gttcgcggag gcgggattcc gggtcggggg acacctagcg
     6721 cagggcctcc tcgtattcgg tgggatcgta gataagaggg tagccgtcgg ccagggtctc
     6781 gcctgcggtg acctcgccga cgatgaccgt gtggtccccg gagcggagtg tgtccatgac
     6841 cctgcagtcg aggtaggcca gacagtcaag gaggaatggc gagccccccg gagtcaggcc
     6901 gtaagggacg ccctggagct tgtcatggta ctcgccactt gtgtccgaga agtgggttgc
     6961 gacgtcgacc tggtcgttgc gcagggcgct cagggcgaag gtcgagctct ccaggatcag
     7021 gtcgtgagag tagcggtctt cgcggacggc gacggccacg cacgggggtc gttcggacgc
     7081 atgcatcacc caggaggcgg tcatcgcgtt ggactggcgg ccgcgcctgg tgccgagcac
     7141 gtagaccccg tgggtcagat tgcgcatcgc gccgagtata gagccgcggt cgtgaggttc
     7201 gctatccacc tttctcctct tccggggagt caacttggag gtattctatc aacatgagtg
     7261 ggtatagatt cttgctgcgt cgctctccgg aggtgtgtcg atgtggaatt gagcgcttcg
     7321 ctcgtctggt tcggggtcct gcagaccctc atctacctgc tctttatcag gaccatcgac
     7381 ctctacgagc gcgagccgct gcgatacgtg ttgtccgtgt tcatctgggg cttcgcggtg
     7441 gctacgacca tctcgctggt cttcaacacc atcgcccaga tcacgttcga tgcgctcgtc
     7501 agcaggcagg aggcgaactt cctgacggcg gtcttcgtgg cgcccgtggt cgaggagtcc
     7561 agcaagggac tcgcgctgtt catcatcttc gtcatcgcct acctggcgtc gcgcaggcgc
     7621 cacctcatcg agttcgcggg tgtgatggac ggcatcgtgt acggttcggc tgtcggtttc
     7681 ggcttcgctc tcgccgagga catactctac gggttgcagt tcggcgcgga gacgttcctg
     7741 gtgcgccgga tcctcggcgg cttcggccac gccgcgttca cctcgttgac gggcatcggg
     7801 atcggcctta ttcccttcgt caacaacaga gcgctgaggg tccttccgcc gttgctcggg
     7861 ctccttggcg caatcctcct gcacgcgacc ttcaacttcc tggccacgac gatgggtcct
     7921 gtagcctacc tatttatgtt tgtcgtcgtg ctcctgtacg tgatgctcat catcgtgtgg
     7981 ctgacaatcg agcggcgctg gatacgggac gagctgcgag gggaggtcga taaaggcatc
     8041 ataagtccgg aggagtacgc cgtcctcccg accgccttcc gcaggactgg atactacgtc
     8101 cggctcatcc tcaggggaca cccggccgag tggtccagcg cgcggaaggt tcacgcggcc
     8161 gccgtggacc tcgccttcgc caaacgcctc gcccgccgct ggcccacacc cacccgccag
     8221 cagcgggtcg aggctctgcg actgaagatc ctggacctga aggcccaggc agcgatccgg
     8281 gtgcgcacct gaaccctcgc accgacgcat ccggggtgat actcgccggc gggaagagcc
     8341 ggaggatggg ccgcgacaag ctcctcctcg aggtgggagg cgagcccctt ctcaaccgcg
     8401 tccacgacgt cctcgccagg cggtgcgcgg aggtgatact ggtaggaggc ggccccgcgc
     8461 ggctcgaagg ggtcaggcag gtgcaggacg agcggatagg cggcctgggg ccgctggccg
     8521 ggatcgaggc gggcctcgcc gccgcagagt accaactcgt cttcgtcgcc gccgccgacc
     8581 tgcctttctt gcccgctgaa ctggtcggct tcgcgctcga gcgtctcgag gaacgcgggg
     8641 cccttgccgt ggtgccccgg cacgggggca ctgcgcaccc gctgtgcgcg gcctacgatc
     8701 gccgtatact gccgcgcgtg cggtcggcgc tcgatggcgg ggtgcgctcc gtacgcgagt
     8761 tcctcgacag tctggaccgg gtagattacg tggaaagaga gttgcggcgg tttggaaatc
     8821 ccgagctatt cctgatgaac gtaaactcgc cggaagacct ggaccgggcg cgggcgggcc
     8881 tacattgaag ccgccgtctt ccaggggcgt tctggagtgg atccgaacgc tggtcgacgt
     8941 ctaccggggt ggcgcgcctc ccccgggcga tgcgcccgcg gagatccagg tcgccgtagt
     9001 gctcggtacg gaggtgctct cgggtgggcg gccgagcagc acgctagcgg cgagggtcag
     9061 gcacgcggcc gggctctacg agcggggcag ggtgcgtctg atcatcgcca ccggcggcct
     9121 cgggaagcat ccgcctgcgg aggccgatgt aatgggacgc gtgctcgggg aggagggggt
     9181 gccggaccac gccgtgatgc tcgaagatag ggctctcaac acgtgggact cggcccggtt
     9241 cgttacgggc atggcgaaga ggctcggcgt aagcgacgtg ctggtggtca ccgacccgct
     9301 gcactgcgtc cggacggtgg cggccttcga gcgggctggg cttggggcgt gggcggagcc
     9361 ggtgtacggc agcccgatgt ggcgcggcag ctgggcgcgg ctggggcagt tcgtcaggga
     9421 gctgggagcg ctggtttggt acaggataag gtacggggta ggtacgcgct cccggcagta
     9481 gcggtcgggg tgctcgccgt ctcggcggcg gccatcttta tccggctcgc cgaggcgccg
     9541 gccatcgccg tggccttctg gcgctgcgcg ctcggggcgg cgctgctgtt gccgcccgcg
     9601 ctcgtgcggc gggatcggtt cccgaggggg cgggacctgt gggtggggat agcgtctggc
     9661 atcgcgctgg gggcgcactt cgggttctgg atctcctccc tcgaccatac gagcgtggcg
     9721 gcgagcgtcg tcctcgtctc cacacagccg gtgttcgtgg cgatcctggc gtatttgatc
     9781 ttcggggagc gtacctcgcc gctctccttc gccggcatcc tggccgccct ggtgggtacg
     9841 gccatcatcg cgggtgacga taccgtgggg tcggcggccc tccttggcaa cgcgctcgcc
     9901 ctcgccgggg cggtaacggt ggcggtctac gtcctcatcg ggcgctattc gcgcaccggg
     9961 ggcattgggg tcctgcccta ttccgtggtg gtgtactccg ccgccgctct cgctctcctg
    10021 cctgtggcgc tcgtcctcga cgtccggctc tggggatact cgggcgagac ctggttctgg
    10081 ctctgggcga taacgctggg cccccagctc atgggccaca ccgtcttcaa ctgggcgctc
    10141 cgctacgtcg aggcgtcggt gatctccggc acgatcctcg ccgagcccgt catctcgtcg
    10201 ctcctcgcct ggctcatact ctccgagacg cccggcctcc cgactatcct cggcggctgc
    10261 atcgtgctca tcggtctctt cctgctcctc cggggacgca aggttcctgc ggagccaata
    10321 ggttaggcag cacgcttcgg ccctttacta aaggccctcg cttctcagca tttcagcttc
    10381 tccgccgttc ggttgacgcc cggtcacgct tggagagacg cttctgggca tatccttgga
    10441 ctgacgcagg gaagatcatg tcgcgtcctc cttgctttta gctgagaagc cgcctgaggc
    10501 gaagccaaag gctgcgtgct gagagcgagg ccctaaaagg gccgaagcgt gctgacaagc
    10561 tgcctgaaag gcagcgtgct gacaaggcgc gaagcgccgt gctgagaagc ggaagccgaa
    10621 ggctggagcg tgctgacaag cggaggcgaa gccggagcgt gctaggtgg
//
LOCUS       DASTUV010000224         6890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_986512, whole genome shotgun sequence.
ACCESSION   DASTUV010000224 DASTUV010000000
VERSION     DASTUV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986512"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFT03_04555"
     CDS             <1..654
                     /locus_tag="VFT03_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU4847447.1"
                     /translation="LIPAARAHGAWVHVDGAFGMWAATSPALRYLTEGMEQADSWATD
                     AHKWLNVPYDCGLVFCARPAAHQAAMGVHASYLVQSEDRERDEMDWNPDFSRRARGFP
                     VYAALRSLGRSGVTDLVDRCCAHARRFAEALAQEPGVEVLNEVVLNQVLVRFLDPGGD
                     HDARTRTVVKAVQEDGTCWLGGTLWQGKAAMRISVSNWSTTAEDVDRSVAAILRAAN"
     gene            856..3618
                     /locus_tag="VFT03_04560"
     CDS             856..3618
                     /locus_tag="VFT03_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU4847448.1"
                     /translation="MTYTAWFDEIRKDDIALAGGKGASLGELSHAGLPVPPGFVLTTR
                     AYDAFVESNDIGEEIVGRASAARAEDPAAFEEVAEGIRALFSSGKVPEEMAHEIRAAY
                     EEMSEDAATPVAVRSSATAEDLPGASFAGQQDTYLNVRGAEALLEAIKSSWASLWTAR
                     AMAYRARQGIDPATVSLAVVVQRMVESESAGVMFTANPANGRRNEVAISAAWGLGESV
                     VSGSVTPDSIVVEKESGRVISREISNKEVLTVYTESGTEERPVPESRRRQPVLDDEMA
                     AELARFGARIEELYGSPQDIEWALAGGELFILQARPITALPEPEADPPTDWSVPDPKA
                     MYVRASIVEQMPDPLSPLFADLADGSVTRSLRTLMNEILGSGTLREGDLRFPTINGYA
                     YYYYKISALLRISAKSLPALPLLYGSGKASGVKRWREYSHPRYATIVEEWETKPPEAL
                     PAVRLLAGARELLDAGTEYYTSVQTVIPLAASSEALFTAFYNRLVRREGDPPAQTFLL
                     GFDSMPIQAEKSLYDLGVWSREHPDLARTLAGTPSDRILDLLNAEEPPSGVDSEVWRE
                     WRSRFQTHLDRYGHAVYNLDFVNPVPADDPAPLVDTLKFYLRGDVTSPHERQRRTAAR
                     REEATRSVLERLDAMRRKLFSRLLDWAQNVAPVREDALADVGLAWPVMRRMLLELGGR
                     LVEAGAVEKPADVFWLRLDELDDMAASLDAGEPRLGNHAEAVEERKMLWRGQRRVTPP
                     QLLPKDSWFMVFEGLMPARSEEQTGDTIKGTAGSGGQVTAPARVLSGPEDFGQMQPGE
                     VLVAAITTPAWTSLFAMAAAVVTDVGGPLSHSSIVAREYGIPAVLGTGVATRRIQSGQ
                     SIRVDGDAGTVTLLDRRHESDAVQPPPERVSRAHSRGARTVALIALAAGAVVAGAFWW
                     RKRSRS"
     gene            3826..4377
                     /locus_tag="VFT03_04565"
     CDS             3826..4377
                     /locus_tag="VFT03_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847449.1"
                     /translation="MTTQAVAAIYGSLVGGVLAMIGVLAGMALERRLQSRGKLRCIVS
                     DWELTDVGSLGQAFCSFEVYLFNERILATGLRDVSVVFYGDGEQGWGYLLKDRASSES
                     LRVIDLPPRQWVHVRPYALFEGKVVQELTGLRQVVLAGHLPDGSEFRQRILAREDFVA
                     TRSEPVSARKDYSRPWWRRMLGR"
     gene            4494..4808
                     /locus_tag="VFT03_04570"
     CDS             4494..4808
                     /locus_tag="VFT03_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847450.1"
                     /translation="MLTITPAAKLRFQTITDVDIPEGWALRLDTPGMNHIGDTPGIAM
                     YVGEPQEDDRPVQHEGETLLYVSGCVAEAYAGFVVDVEEHPEGLRFGIGLPDVGSMEE
                     PG"
     gene            4973..5269
                     /locus_tag="VFT03_04575"
     CDS             4973..5269
                     /locus_tag="VFT03_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847451.1"
                     /translation="MVWPFVDPLPIEQGKTREQKGVTEMYEPRRNAATLLDAQLRFAE
                     MVVDACAQGAKMYWAIWGPMGGMAIDAVEALESTQHRYLERLRDAVEDGNAVRS"
     gene            5369..5566
                     /locus_tag="VFT03_04580"
     CDS             5369..5566
                     /locus_tag="VFT03_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847452.1"
                     /translation="MSYTPEDRIREEQVRRLSADEDPRGSQHRDYSYTVDLKPKKAKT
                     RDREKEERVRARLLAHEQASD"
     gene            5969..>6890
                     /locus_tag="VFT03_04585"
     CDS             5969..>6890
                     /locus_tag="VFT03_04585"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU4847453.1"
                     /translation="MSLFPAHPESGTAILPSEGRLPPFEGATGWLNSEPLTPEGLRGR
                     VVAVQFWTYTCVNWLRTLPYVREWAKKYHDHGLSVIGVHTPEFGFERDVENVKAAALE
                     MRVDYPIALDSNYAVWRAFANHYWPALYIADEQGVIRYHHFGEGEYAMSEMVIQQLLG
                     EAGKEGFDPAFVFVEPQGTEVAADWTNVRTPETYLAYGRSYGFASPDGEYFEEPHDYP
                     EPSSPLSLNEWAPSGLWTLAEHAAVLGEAPGRIAIRFHARDVNLVMGPAERGTSVRFR
                     VLLDGEVPGDAHGTDVDEQGKGTLSEQRLYQ"
BASE COUNT         1302 a         2105 c         2318 g         1165 t
ORIGIN      
        1 ctgatccccg ctgcccgagc gcatggagct tgggtccatg tggacggggc gttcgggatg
       61 tgggcggcca ccagcccggc cttgcggtat ctcacggaag gtatggagca ggccgactcc
      121 tgggccaccg acgcacacaa gtggcttaac gtcccctacg actgtggtct cgtcttctgc
      181 gctcgcccgg ctgcgcacca ggcggcgatg ggcgtccatg cgagctacct ggttcaatca
      241 gaggaccgcg agcgggacga gatggactgg aaccccgact tctcgcgtcg ggctcggggc
      301 ttccccgtgt acgcggcact gcgctcgctc ggccggtcgg gcgtcacgga tctcgtcgat
      361 cgctgctgcg ctcacgcccg acgctttgcc gaggccctcg cccaagagcc gggggtcgag
      421 gtgctcaacg aggtagtcct gaaccaggtg ctcgtgcgct tcctcgatcc aggtggggac
      481 cacgacgccc gcacccgcac cgtcgtgaag gccgtgcagg aggacggaac ctgctggcta
      541 ggaggcaccc tctggcaagg gaaagcggcc atgaggatct cggtgtccaa ctggtccact
      601 actgcagagg atgtcgaccg ctccgtcgca gcgatcctgc gcgctgccaa ctaggaggct
      661 gttccagcag ggagctcgtc tcctgacaat gcgctcgtat cgcggcgaag aattcccgcg
      721 ggagtgggat ccctatgata ggtaagttgg agctacgtcg ccggcggcgc gggacatacg
      781 ccgtggatga gccataagaa agagtatctc tccgtcatca acgagttcct ggaggagtag
      841 gaaggggcag gcgtcatgac ctacacggcc tggttcgacg agatacgcaa agacgacatc
      901 gctctggcgg gcggcaaggg cgccagcctg ggcgagttga gccacgcggg actacccgta
      961 ccgcccggct tcgtcctcac caccagggcg tacgacgcat tcgtcgagtc aaacgacatc
     1021 ggagaggaga tcgtcgggcg cgcctcggca gcccgggcgg aggatccggc cgcgttcgag
     1081 gaggtagcgg aggggatccg tgcgcttttc tccagcggca aggttcccga ggaaatggca
     1141 cacgagatac gcgccgccta cgaagagatg agcgaagacg cagcgacgcc cgtcgccgtc
     1201 cgatcttctg ccaccgccga ggacctgcca ggcgcgagct tcgccggcca gcaggacacg
     1261 tacctcaacg tgcgcggagc agaggcgctg ctcgaggcga tcaagagctc gtgggcttct
     1321 ctgtggacgg cacgagcgat ggcttaccgg gcacgccagg gcatagatcc tgccaccgtg
     1381 agcctcgccg tggtggtcca gaggatggtc gagtccgagt cggctggcgt catgttcacg
     1441 gccaaccccg cgaacggtcg ccggaacgag gtagcgatca gcgccgcctg gggcctgggc
     1501 gagtcggtgg tgagcggctc ggtgacgccg gactccatag tcgtggagaa agagagcggg
     1561 cgcgtgatct cgcgcgagat ctccaacaaa gaggttttga ccgtatacac cgagagcggc
     1621 actgaggagc gacccgtgcc tgagtcacgg cgccgccagc cggtgctcga cgacgagatg
     1681 gcggcggagc tggcgcgctt tggagcgcgc atcgaggagc tgtacggatc tccgcaggac
     1741 atcgagtggg cgctcgcggg cggggagctc ttcatcctcc aggcacggcc catcaccgcc
     1801 ttgccggagc cggaggccga tcccccgacc gactggagcg tgcctgaccc gaaggccatg
     1861 tacgtgcgtg ccagtatcgt cgagcagatg cccgacccgc tctcaccctt gttcgccgac
     1921 ctcgcggacg gatcggtcac ccggtccctg cgaacgctca tgaacgagat cctcggttcc
     1981 ggcaccctca gggagggcga cctgcgtttc ccgaccatca acggctacgc ctactattac
     2041 tacaagatct cggccctcct gaggatcagc gccaagtccc tcccggccct cccgttactg
     2101 tacggaagtg gaaaggcaag cggcgtaaaa cgctggcgcg agtactctca cccccgctac
     2161 gcgacgattg tcgaagagtg ggaaacgaag ccgccagaag ccctgccagc agtccggctc
     2221 cttgcgggcg cgcgggagct actggacgcg gggaccgagt actacacctc ggtgcagacg
     2281 gtcatcccgc ttgccgcttc gagcgaggcg ctgttcaccg cgttctataa ccggctcgtc
     2341 aggcgcgaag gggatccccc ggcacagacc ttcctgctgg gtttcgacag catgcccatc
     2401 caggccgaga agtccctcta cgacctgggt gtctggtccc gcgagcaccc cgacctcgcc
     2461 cggacgctgg ccggcacacc gtcggaccgg atcctcgatt tgctcaacgc tgaagagccg
     2521 ccctccgggg tggacagcga agtgtggcgc gagtggaggt ctcgcttcca gactcacctc
     2581 gaccgctacg ggcacgccgt ctacaacctg gacttcgtga accccgttcc cgcagacgac
     2641 cccgcgcccc tcgtcgacac gctcaagttc tacctccggg gcgatgtaac gagtccccac
     2701 gagcgtcaga ggaggacagc cgcgcgccgc gaggaggcga caaggtcggt cctggagagg
     2761 ttggacgcca tgcggcgaaa gctctttagc aggttgctcg actgggcgca gaacgtagca
     2821 cccgtgcgag aggacgcgct cgccgacgtg gggctggcct ggcccgttat gcggcgtatg
     2881 ctcctcgagc tgggcggccg cctggtcgag gcgggcgccg tggagaaacc cgccgacgtg
     2941 ttctggctgc gcctggacga gctagatgac atggccgcgt ccctggacgc gggcgagcca
     3001 cgcctcggca atcacgctga ggccgtggag gagcgcaaga tgctctggcg gggacagagg
     3061 cgcgtcacgc ctccgcagct actccctaaa gactcctggt tcatggtctt cgaaggactg
     3121 atgccggcga gatcggagga gcagaccggc gacaccatca agggcacagc cggtagtgga
     3181 ggccaggtca cggccccggc gcgcgtactc tctggcccgg aggacttcgg ccagatgcaa
     3241 cctggggagg tcttggtggc ggccatcacc accccggcct ggacctcgct cttcgccatg
     3301 gccgcagcgg tcgtcaccga cgtcggcgga cctttgagcc acagctcgat cgtggcacgc
     3361 gagtacggca tcccggccgt gctcgggacc ggcgtggcga cccgccgcat ccagagcgga
     3421 cagagcatcc gcgtcgatgg tgacgccggc acggtaacgc tgctcgaccg gaggcatgag
     3481 tcggacgccg tgcagccacc gccggagcgc gtctccaggg cacacagccg gggcgcaagg
     3541 acggttgcac tcatcgcact ggctgccgga gcagtggtag ctggtgcctt ctggtggaga
     3601 aaacggagcc ggagctagac ggcagaagtc ctgacgtcgg tactcgttct tcccctgatt
     3661 atgcgcaccg ctgcggcgct tcaatgcccg gctggccagg atatggtgcc cttgcgctcg
     3721 gcctcttgca gtgaggagta acataccggc tatggaggac aggagtctgc gctccgatgg
     3781 tgagctcgag cgggagaacg agtatccgag ggggtcgatg agcggatgac cacccaggct
     3841 gtcgcggcaa tctacggtag cctggtaggg ggggtgctcg cgatgatcgg cgtactggcg
     3901 ggcatggctc tggagaggcg tctgcaaagt cgcgggaagc tgcgctgcat agtatctgat
     3961 tgggaattga cggatgtggg ttcgttgggg caggccttct gttcgtttga ggtctacctc
     4021 ttcaacgaga ggatcctggc aacggggctc cgcgacgtct ccgtggtgtt ctatggggac
     4081 ggcgaacagg gttggggtta ccttctcaag gatcgggctt cctcggaaag cttgagggtg
     4141 atcgacttgc cgccccggca gtgggtgcac gtgagaccgt acgccctgtt cgagggcaaa
     4201 gtagtgcaag agctcaccgg gctccggcag gttgtactgg ccggtcacct acccgatggc
     4261 agtgaattca ggcagaggat cctggctcgc gaggacttcg tcgctacccg aagcgaaccg
     4321 gtgagtgccc gcaaggacta ctctcggccc tggtggcgca ggatgctagg aagatagggt
     4381 aagtccgctc gaaaataact ccagtactgg gacatatgcc ccatgtacct tccactcact
     4441 cgtagtagaa agagacttcc ctggaggaaa agaggggagg acccagacac cgtatgctca
     4501 cgatcactcc ggcggccaag cttcgctttc agaccataac ggacgtagat atccctgaag
     4561 ggtgggcact ccgcctggat acgcccggta tgaaccatat cggcgacaca ccgggcatcg
     4621 ccatgtacgt aggagagccc caggaagatg acaggcccgt ccagcatgaa ggagaaacgc
     4681 tgttgtacgt ctccggctgc gtggccgagg cctacgccgg gttcgtcgtc gacgtcgagg
     4741 agcaccccga gggcctacgc ttcggtattg ggctgcccga cgtcggctcc atggaggagc
     4801 caggctgaag tagcgctcat cggcgcgagg gtgcgtgctc gcctcctgaa ggtcgggccc
     4861 cagaggctat cacaggaagg tgaggccacc tagattacat ggccctggac gtgctcctgg
     4921 acgatggcag cacgaactcg aggaggtcga gtaggaggat gtcgagaagc tcatggtctg
     4981 gcccttcgtc gaccccttac caatcgagca aggaaaaacc cgcgaacaga aaggagtgac
     5041 ggagatgtac gagccccgca gaaacgcagc gacactgctc gacgcacagc tgcgtttcgc
     5101 cgagatggtg gtcgacgcct gtgcccaggg cgcgaagatg tactgggcta tctgggggcc
     5161 tatgggcggt atggcaatcg atgccgtgga agcgctggaa agcacgcagc atcgatacct
     5221 cgagcgactg agggacgccg tggaggacgg gaacgcggtt cggtcctgat ccgctgctcg
     5281 ccggtgttat cgtagccggc actcgaggta cgtagttcct gacgacagac actggaagcg
     5341 tcgtgaccga aggaggagcg agaagctcat gtcctacacg cccgaggacc gcatccgcga
     5401 ggaacaggtc cgcaggctca gcgccgacga ggatcctcgt ggcagccagc atcgggacta
     5461 tagctatacg gtcgacctca agccaaagaa ggcgaagacc cgcgaccggg agaaggagga
     5521 gagagtgcgc gccaggctgc tggcccacga acaggccagc gactgatcaa ttcgccaaat
     5581 cctgctcctt aacgcttcgc agcaccgctg gcgtgagaga ggaacctgcc tggctgcatc
     5641 ctatcgcctc gcacctcagg gagagcttcg gtagaggagt gccgctctaa gataccttct
     5701 tcccaaggat agctaatgtg ccccatgaca ctttgtgctg tgtggaacac acaccctctc
     5761 tacaaaggag tatggtgcgg tgtctgtggt acggatgcgc ggttcaggga aaggagagcg
     5821 ccatgaccag tccagttggg gaaggggtcg cagtccgccc cttccgcacc gacgtgtcgg
     5881 aagaggaact cgtcgacctt cgccgacgca tcgcggcggc gctgacggag ttcctggtcc
     5941 cgtaccgcga aagcggaggt ggcttgcgat gagcctcttc cccgcgcatc cggagagcgg
     6001 aacggccatc ttgccgagcg agggccgcct gccgcccttc gagggcgcga ccggatggct
     6061 caactccgag ccgctcacac ccgaggggct ccgaggtcgc gtcgtcgcgg tgcagttctg
     6121 gacctacacg tgcgtcaact ggcttagaac gctgccctac gtgcgtgagt gggccaagaa
     6181 ataccacgat cacggcctga gcgtgatcgg ggttcacacg cccgagttcg gcttcgagag
     6241 agatgtcgag aacgtgaagg cggccgccct ggagatgagg gtagattacc cgattgccct
     6301 agatagcaac tacgccgtct ggagagcctt cgccaatcac tactggccgg cgctctacat
     6361 cgccgatgag cagggagtga tccgctacca ccacttcggc gagggtgagt acgccatgtc
     6421 tgagatggtt attcaacagc ttcttggtga agcgggcaag gaaggcttcg atccggcgtt
     6481 cgtgttcgtt gagccccagg gcacggaggt ggcggcggat tggaccaatg tgaggacgcc
     6541 cgagacatac cttgcctatg ggcgctcata cggcttcgct tctccggacg gtgagtactt
     6601 cgaggagcct cacgactatc cggagccatc gtcgcctctg agcttgaatg agtgggcccc
     6661 ctcaggcctc tggacgcttg ccgagcacgc ggcagtgcta ggcgaggcgc ccggacggat
     6721 tgctattcgg ttccatgcgc gcgatgtgaa cctcgttatg ggacctgcag agcgaggaac
     6781 ttccgtgcga tttcgcgtgc tcctcgatgg agaggtgccg ggagatgctc acggaactga
     6841 tgttgacgag cagggcaagg gcacgctcag tgaacagcgg ctgtatcagc
//
LOCUS       DASTUV010000225         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1006432, whole genome shotgun sequence.
ACCESSION   DASTUV010000225 DASTUV010000000
VERSION     DASTUV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006432"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFT03_04590"
     CDS             complement(<1..289)
                     /locus_tag="VFT03_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4847454.1"
                     /translation="MPEFDPIIGRYAYLEVSGARNRVYFEEAGEGRPLLCLHTAGADS
                     RQWRHLLCDTEVTGRWRVLAFDMPYHGRSTPPAGWWEKEYLLTTGTYARMIM"
     gene            complement(282..731)
                     /locus_tag="VFT03_04595"
     CDS             complement(282..731)
                     /locus_tag="VFT03_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847455.1"
                     /translation="MGRESSTEARTGPGPALARAVFPERSWFEALGEAVAADAEMAVI
                     GRWCTLDLALVADEEMVLLRLRAGRISEVVPNPGISYSWSVTLRGTRKDWLTFLQPEP
                     PPFYNDLLAMNGRVPSFSIEGDRAAFVRHLRTLSRLFRLAQLMGSHA"
     gene            complement(724..1665)
                     /locus_tag="VFT03_04600"
     CDS             complement(724..1665)
                     /locus_tag="VFT03_04600"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4847456.1"
                     /translation="MEPLCRSKYHRRGTMTPKLGCVGLGNIGEPVARHLLLAGYEVLV
                     HDTSAEAVSRLDDTPARPVGDLKALASAADAVLLSLPNSTVVEEVVLGRGGLVEGLSA
                     GKILIDTSSSRPSSTRTIAKKLAEEGADMLDAPVSGGVLRAKEGTLSVMVGGKQEVFE
                     RCYPILEAFGGKIFYVGDHGAGHLVKSLNNLLSATTLASAAEALILAQRAGVSPETFV
                     EVINASNGRSYSTEVKLPRYVLDASFDDGFALGLMVKDLKIALESAEEIGLPMFVGST
                     LGQIWQAANAQGYGAEGHTAIYAFLEELAVPGRSRNG"
BASE COUNT          394 a          726 c          656 g          386 t
ORIGIN      
        1 ccattatcat gcgcgcgtag gtcccggtcg tgagcaggta ttccttctcc caccaacccg
       61 ccggcggcgt cgaccgcccg tggtagggca tgtcgaaggc gaggactcgc cacctaccgg
      121 tcacctccgt gtcgcagagc aggtgccgcc actggcgcga gtcggccccc gctgtgtgga
      181 ggcacaggag cggacggccc tccccagctt cctcgaagta gacgcggttg cgggcgcccg
      241 agacctccag atacgcgtat cggcctatga tcgggtcaaa ctcaggcatg gctacccatc
      301 agctgcgcga gacggaacaa cctgctcaag gtgcgcagat gtcgcacgaa agccgcgcgg
      361 tcgccctcta tggagaacga tgggacgcgg ccgttcatgg cgagcaggtc gttgtagaag
      421 gggggaggct ccggctgcaa gaaggtcagc cagtcctttc tcgtgccgcg tagcgtcacg
      481 ctccaggagt agcttatgcc ggggttcggg acgacctcgg agatacggcc tgcccgcagg
      541 cgcagcagga ccatctcctc gtcggccacc agggcaagat cgagggtgca ccaccgccct
      601 atcactgcca tctcggcatc cgccgcgacc gcctcgccca gagcctcgaa ccagctccgc
      661 tccggaaaca cagctcgggc gagcgccgga cctggccccg tcctagcttc ggtggacgac
      721 tctctaccca ttacgactcc ttcctggtac agcgagttcc tcgaggaagg cgtagatcgc
      781 cgtatggccc tccgcaccgt acccctgcgc gttggcggcc tgccagatct gtcccagcgt
      841 ggagcccacg aacatcggta gcccgatctc ctcggcgctc tcgagggcga tcttgaggtc
      901 cttgaccatg agcccaaggg caaagccgtc gtcgaaggat gcgtcaagaa cgtagcgggg
      961 tagtttcacc tccgtcgagt agctgcgccc gttgctcgcg tttatcacct ccacgaacgt
     1021 ctcaggggag acaccggccc tctgggccag gatcagcgcc tccgcggcgc tggcgagggt
     1081 ggttgccgag agcaggttgt tgagtgactt gaccaggtgc ccggccccgt ggtcgcccac
     1141 gtagaagatc ttgccgccaa acgcctcgag gatcgggtag catcgttcga agacctcctg
     1201 cttgcccccg accatcaccg acaaagttcc ctctttggcc cgcagcacgc caccgctcac
     1261 cggggcgtcg agcatgtcag cgccctcctc tgcgagcttc ttcgcaatgg tccgcgtcga
     1321 ggacggtctc gagctcgacg tgtcgattag gatcttgccg gcggagagcc cctccaccag
     1381 gccacccctg ccgaggacga cctcctccac gacggtcgag ttcgggagtg agagcagaac
     1441 ggcgtcggcg gcggaagcaa gagcctttaa gtctccaacc ggcctggcgg gggtgtcgtc
     1501 gagcctggag acagcttccg cgcttgtatc gtggaccagc acctcgtacc ctgccaggag
     1561 cagatgtctt gctactggct cgcctatgtt gccgagcccg acacaaccta gctttggtgt
     1621 catagttccc ctccgatggt atttcgagcg gcagagcggc tccacctgac cggggctgca
     1681 gcgggatcga agagacagcc gagatctctt atgatggcct ctagagccgg aggggctcgg
     1741 gtgaaagacg gttgtctgtc ttctcgcgcg atcaatactc ccgcgatatc caatagcgag
     1801 aagtagtcgg tcgcctccgg caacccgtcg tcgaccacca tgatcgtcat cctccctcta
     1861 caaaagatgt accgctatct ctggtgacga cgacgaagac ccgcaccttc tcgctcacgg
     1921 caacagacct ccctcttcct aagcggcggc attaccccga gaaatgccag gtgctacgcg
     1981 ccaggccgct cgcaccaagt tccagtcagg ggattgaggc ctctctgcgg agcgatctgg
     2041 cccgcagatc tcaggcgtcc atgccacgca gaggagcacc cttcctgatg ggagtccgct
     2101 acaccagtgg gatcggcatc caggaactcg gtcgagtctc tggctcgcga accaagggac
     2161 gc
//
LOCUS       DASTUV010000226         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_765364, whole genome shotgun sequence.
ACCESSION   DASTUV010000226 DASTUV010000000
VERSION     DASTUV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765364"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1196)
                     /locus_tag="VFT03_04605"
                     /pseudo
     CDS             complement(1..1196)
                     /locus_tag="VFT03_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
     gene            complement(1346..>2058)
                     /locus_tag="VFT03_04610"
     CDS             complement(1346..>2058)
                     /locus_tag="VFT03_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU4847457.1"
                     /translation="PEGMIRAFDDRHGLYVLHAWGMTETTPLGTICNLSSQMDEEPED
                     ERYRIRATQGLPGAFIEVRARGDEGFVAWDGESMGELEIRGPWVSGAYFNTEEGDDKF
                     TEDGWFRTGDIVTIEPSGYVEIQDRTKDLIKSGGEWISSVALENSLMAHDAVAEAAVI
                     AIPDEQWGERPLGVVVLKEGEEATEEELLSHLASDHPKWSLPDAIEFVEEIPHTATGK
                     ALKMALRERYSDYTSAKS"
BASE COUNT          307 a          716 c          658 g          377 t
ORIGIN      
        1 gtggggacgg ggtccctcgg gtcgtagtcg tactcgtccg gctcgccccg ctccggcgct
       61 tcgcgggcca ggcgcccgcc gccgtgcagg tgccatcgtt cttcgtacgc gccggggacg
      121 ggccactcct cgtagccgcg ccagcggttc tcgcccatca cgaaaacctc gaccggcagc
      181 ctctcttcca gtgaccgctc atcaccttta agcgtcgcgt cgaaccagcg gaggtgggcc
      241 tctgtgagat cgccgcggta gttcaggaac gcgccggagc tgcctatccc gaagtccagg
      301 tcgccgatgg tgctcccgaa ctccgcgtgc gtccagggcc ccacgagcag acgcgacggg
      361 cgcgtgcctg ctgccttcgc ccgctccttc atcacctcct actgcctcag agtctccccg
      421 ataaagcagt cgtaccagcc cccaatgtgg aaggtcggcg cctcgacgcg gtcgtacctg
      481 ccgtcgatgt tgaggtactc ccagctctcg tcgtccgcgc cgcgcgcgaa gccgccgcgc
      541 acgaacggcg tgaggtcgtc ggggtcgggg agctgcgtga gcgggagcgc atcgtagccg
      601 ccgccagcga gcagcgtatc tatggagcca accagctcgg gcaacttctc tgcaatctcc
      661 cgaggctcgc cgcggtactt gcggaagagg accccgagcg cgagggcgcc ctgcgcccag
      721 tactgtatga ggcccagctc ccgcgctccg ccgcgcatct gggcgccgtt caggtggttg
      781 ccccatgtct ggccgggggc catgcttttc aaggagggcg gctgcatgac ggccgcgtgc
      841 cactgggcct tgccgaggta ggaaagccct atcatcccca cgctgccgga ggagccgggt
      901 agcttcgcgg cccactcgac ggagtcgtag ccgtcctcga actcgcgcac gaaaggggtg
      961 aatttaccct ccgaggcgta gcggccgcgg acgtcctgga tcgcgacgat gtagccgcgc
     1021 cggacggccc tcacgggatc gaggtaggag gagtcgaagg ggaggtcttt gccgtatggc
     1081 atgcgggcca ggagaacagg gtacgggccg ccctcagccg ggcggtagac gttggacatc
     1141 agggtcgtgc cgtcgcgcat ccgcgccggt acgtccgtct ggatcgtcac ctccatcgtg
     1201 gggtccaagc gtcctccgtc aggcttccga tctacgatcc ggcgcgtctg ccgagaagcg
     1261 cgaaggctgt cgcggctaaa ctctcgagtg ccacctttaa cgacagcgtg ccggttcgtc
     1321 cgaagccagc ctctatgacc ttcattcagc tcttcgcgga cgtgtagtcg ctgtagcgct
     1381 cgcgcagcgc catcttgagc gccttgccgg tggcagtgtg cggtatctcc tcgacgaact
     1441 cgatcgcgtc cggcagcgac cacttcgggt ggtcggatgc caggtgagag aggagctctt
     1501 cctccgtcgc ctcttcaccc tccttgagga ccacaacccc gagaggccgc tcgccccact
     1561 gctcgtcggg gatggcgatg acagcggcct cggcgacggc gtcgtgcgcc atcagggagt
     1621 tttcgagggc cacggaggag atccactccc cgccgctctt gatcaggtcc ttggtcctgt
     1681 cctgtatctc gacgtagccg ctcggctcga tggtgacgat gtcgccggtc ctgaaccagc
     1741 cgtcctcggt gaacttgtcg tcgccctcct cggtgttgaa gtacgccccc gagacccagg
     1801 gacccctgat ctccagctcg cccatcgact cgccgtccca cgcgacgaag ccctcgtcgc
     1861 cgcgcgccct tacctcgata aatgccccgg gtaggccctg ggtggctctt atcctgtacc
     1921 gctcgtcctc cggctcttca tccatctggc tgctcagatt gcagatggtc cccagaggag
     1981 tagtctcggt catgccccag gcgtggagca cgtacagccc gtggcgatcg tcgaaggccc
     2041 ggatcatgcc ctctggag
//
LOCUS       DASTUV010000227         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_14621, whole genome shotgun sequence.
ACCESSION   DASTUV010000227 DASTUV010000000
VERSION     DASTUV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14621"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFT03_04615"
     CDS             <1..270
                     /locus_tag="VFT03_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847458.1"
                     /translation="RVGRVKSYESIVKGENIPEPGVPASFKVLLKEMQSLGLSVKPVY
                     NAEAAAEDQDRWDWDEAARALGINLSRDEPTGNLDDAPDFSRGGI"
     gene            274..>3928
                     /locus_tag="VFT03_04620"
     CDS             274..>3928
                     /locus_tag="VFT03_04620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU4847459.1"
                     /translation="MQGLERDIDINKLEKISIGLASAEEIREWSRGEVTKPETINYRT
                     LRPEKDGLFDERIFGPSKDWECYCGKYKRIRFKGIICERCGVEVTRARVRRDRMGHIE
                     LAAPIAHVWFVKGVPSRMGYLLDISPKELDRVLYFASSIVTWVDREERANDIDLLRGR
                     VEEEIRQLEEERDEEVDQAQALRTKRESVINGDSSPDELTEEYADLPDEEREAAIARV
                     RKEAEEAIADIQRSVQEQIELIRRAWEEFQTLEPRQIVDDEELFREMKDRFGDEYGYG
                     VYFRGGMGAEAIRDLITQVDLEDEAQELRDTVNESRGQKRQKAIKRLKVVDAFRTSGN
                     RPEWMIMDAIPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLRRLLDLGAPDV
                     IVNNEKRMLQEAVDALFDNGRRGRAVTGAGNRALKSLSDMLKGKQGRFRQNLLGKRVD
                     YSGRSVIMVGPGLKMHQCGLPRLMAVELFKPFVMKVLVDRALAPNIRSAKQMVERLRP
                     EVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQVHPLVCAAFNADFDGD
                     QMAVHVPLSPEAQAEARVLMLSTQNILLPANGFPIAVPSQDMVLGLYYITYADDDFEE
                     AQPKAYLSSYDEAEAAYKEGTLKIHDKVLYRRAGGRVEATLGRVLFNENIEQTLRDYL
                     GEAYEPEGYEFVNHVLKKGEIKDLVQHYVERYPTELVSRLLDTLKRVGFHTATRAGIS
                     VGKNDIVVPEQKDEILARYQAMVDEVEDQWDQGFISDDERLERVITLWTEAKNEVEDA
                     MKANLWRLNPIYMMANSGARGNYSQITQLAGMRGLMTNTKGEIIDEPVKSNFMEGLSV
                     LEYFTSTHGARKGQADTALRTADSGYLTRRLVDVAQDVVIHDEDCGTEGYVDLPLYLD
                     GGRGDANDSIAARFLARDLVNPETGEVVAEAGTDITPRLLASWREELPRETIVPVRTP
                     SKCESPQGVCQYCYGRALSTYRPVDVGEAVGIIAAQSIGEPGTQLTMRTFHTGGIAGE
                     DITAGLPRVVELFEARHPKAEAVLAEIDGRVSLEEADSDRMIKVVITSPDGEESKQYT
                     VERQLLRPEFVEGAEVRANTPITEGAFYPHAILENDLRYGRGTTTTERYLVDEVQKVY
                     RAQGVDIHDKHIEVIVRQMLRKVVVDDPGDTNLLPEQVADRFTVLAENERVEAEEGKP
                     ATYHTVLMGITKAS"
BASE COUNT          794 a         1259 c         1293 g          582 t
ORIGIN      
        1 cgggtcggta gggtcaagag ctacgagtcg attgtcaagg gcgagaacat acccgagcca
       61 ggggtaccgg caagcttcaa ggtgctcttg aaggagatgc agtccttggg gctctcggtc
      121 aagccggtct acaacgccga ggcggcagca gaggatcagg accgctggga ttgggacgag
      181 gcagcccggg cgcttgggat aaacctcagc cgcgacgagc ctacgggcaa tttggacgac
      241 gcaccggact tctccagagg agggatatag gcgttgcagg gactagagcg cgacatagac
      301 ataaacaagc tggagaagat ctccatcggc ctcgcttcgg ccgaggagat ccgggagtgg
      361 tcccgcggcg aggtcaccaa gcccgagacc atcaactata gaactctgcg tccggagaag
      421 gacggcctct tcgacgagcg catcttcggt ccttcgaagg actgggagtg ctactgcggc
      481 aagtacaaga ggatccgctt caagggcatc atctgtgaga ggtgcggggt cgaggtaacc
      541 cgcgcgcgtg tcaggcgcga caggatgggt cacatcgagc tcgcggcccc catagcccac
      601 gtgtggtttg tgaagggcgt tccgagccgg atgggctacc tgctggacat cagcccgaag
      661 gagctcgata gggtcctcta cttcgctagc tccatcgtca cgtgggtcga ccgtgaggag
      721 cgggccaacg acatagacct gctgcgcggc agggtagagg aggagatccg ccagctcgaa
      781 gaggagcgcg acgaggaggt cgaccaggcc caggcgctca ggaccaagcg cgagagcgtc
      841 ataaacggtg acagctcccc tgacgagctg accgaggagt acgccgacct ccccgacgag
      901 gagcgggagg ccgcgatagc tcgcgtccgc aaggaggccg aggaggccat cgcggacatc
      961 cagcgcagcg tgcaggagca gatagagctc atccggcgcg cctgggagga gttccagacg
     1021 ctcgagccgc gtcagatcgt cgacgacgag gagctcttcc gcgagatgaa ggaccgcttc
     1081 ggcgacgagt acggctacgg cgtgtacttc cgcggcggca tgggcgcgga ggccataagg
     1141 gacctcatta cccaggtcga cctcgaggat gaggcccagg agctgcgcga cacggtcaac
     1201 gagtcgcgcg gccagaagcg ccagaaggcc atcaagcgcc tgaaggtcgt ggacgccttc
     1261 cgcaccagcg gcaaccgtcc cgagtggatg atcatggacg ccatccccgt cctgcccccc
     1321 gacctgcggc cgatggtcca gctcgacggc gggcgcttcg ccacgagcga cctgaatgat
     1381 ctctacaggc gcgtcataaa caggaacaac aggctccgcc gcctgctcga cctcggggct
     1441 ccggacgtga tcgtgaacaa cgagaagcgg atgctccagg aggccgtcga cgccctcttc
     1501 gacaacggga gaaggggtcg ggccgtaacg ggcgccggaa accgcgccct caagagcctc
     1561 tccgacatgc tcaagggcaa gcagggccgc ttccggcaga acctgcttgg aaagcgcgtg
     1621 gattactcgg gccgctcggt catcatggtc ggtcccggcc tcaagatgca ccagtgcggt
     1681 ctgccgcgcc tcatggcggt cgagctcttc aagccgttcg tcatgaaggt cctggtggac
     1741 agggcgctgg ccccgaacat ccggagcgcc aagcagatgg tcgagaggtt gcgccccgag
     1801 gtctgggacg tgctcgagga cgtcatcaaa gagcacccgg tccttctgaa ccgcgctcct
     1861 accctccacc gtctgggcat ccaggctttc gagcctgtgc tcgtcgaggg caaggcgatt
     1921 caggtccacc ccctggtatg cgcggcgttc aacgcggact tcgacggcga ccagatggcg
     1981 gtgcacgttc ccctgagccc ggaggcgcag gccgaggccc gcgtcctgat gctctcgacg
     2041 cagaacatcc tgctcccggc gaacgggttc ccgatcgcgg tcccctcgca ggacatggtg
     2101 ctcgggctct actacatcac ctacgccgac gacgacttcg aggaggcgca gcccaaggct
     2161 tacctctcct cctacgacga ggccgaggcg gcctacaagg agggcaccct caagatccac
     2221 gacaaggtcc tgtaccgccg cgccggcggg agggtcgagg ctaccctcgg gcgtgttctc
     2281 ttcaacgaga acatcgagca gaccttgcga gactacctcg gcgaggccta cgaaccggag
     2341 ggctacgagt tcgtcaacca cgtgttgaag aagggtgaga tcaaggacct cgtccagcac
     2401 tacgtggagc gctacccgac cgagctcgtt agccggcttc tcgacaccct caagagggta
     2461 gggttccaca ctgccacgcg ggcaggcatc tccgtcggca agaacgacat agtcgtgccg
     2521 gagcagaagg acgagatcct cgcgcgctac caggccatgg tcgatgaggt cgaggatcag
     2581 tgggatcagg gcttcatctc cgacgacgag aggctggagc gcgtcatcac cctgtggacc
     2641 gaggccaaga acgaggtcga ggacgccatg aaggcgaacc tctggcgcct gaacccgatc
     2701 tacatgatgg ccaactccgg cgctcgtggt aactactccc agatcacgca gctcgccggc
     2761 atgcgcggcc tgatgacgaa cacgaagggc gagatcatcg acgagcccgt gaagtccaac
     2821 ttcatggagg gcctctcggt actggagtac ttcacctcca cccacggcgc ccggaagggc
     2881 caggccgata ccgctctgcg taccgccgac tcgggctacc tcacgcgacg cctcgtggac
     2941 gtagcccagg atgtggtgat ccacgacgag gactgcggca cggaagggta cgtcgacctg
     3001 ccgctctacc tcgacggtgg ccgcggcgac gccaacgact ctatcgcggc ccgcttcctc
     3061 gcccgcgacc tagtgaaccc cgaaacaggg gaggtcgtcg ccgaggccgg caccgacatc
     3121 acgccgcgcc tgctcgcctc ctggcgcgag gagctgccgc gggagaccat agtccccgtc
     3181 aggacaccct cgaagtgcga gagcccgcag ggcgtgtgcc agtactgcta cggtagggcc
     3241 ctctcgacgt acaggcccgt ggacgtgggc gaggccgtcg gtatcatcgc ggcccaatcc
     3301 atcggcgagc cgggaaccca gctcacgatg cgcaccttcc acaccggcgg catcgccggc
     3361 gaggacatca cggcgggcct tccacgggtt gtcgagctct tcgaggccag gcaccccaag
     3421 gccgaggcgg tgctcgcgga gatagacggg cgcgtgagcc tcgaggaggc cgactcggac
     3481 cgcatgatca aggtggtcat cacctccccc gacggtgagg agagcaagca gtacacggtc
     3541 gagcgccagc tgcttcggcc cgaattcgtc gagggcgccg aggtccgggc gaacaccccg
     3601 atcacagagg gtgccttcta tccacacgcg atcctcgaga acgacctgcg ctacggccgc
     3661 ggaaccacga ccaccgagcg ctacctggtg gacgaggtgc agaaggtcta cagggcccag
     3721 ggcgtggata tccacgacaa gcacatcgag gtcatcgtcc ggcagatgct ccgtaaggta
     3781 gtcgtggacg accccggcga tacgaacctg ctccccgagc aggtcgccga ccgtttcacg
     3841 gttctcgccg agaacgagcg cgtcgaagcg gaggaaggca agcccgctac ctaccatacg
     3901 gttctgatgg gcatcaccaa ggcctcgc
//
LOCUS       DASTUV010000228         4620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_174561, whole genome shotgun sequence.
ACCESSION   DASTUV010000228 DASTUV010000000
VERSION     DASTUV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174561"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2116)
                     /gene="ftsH"
                     /locus_tag="VFT03_04625"
     CDS             complement(71..2116)
                     /gene="ftsH"
                     /locus_tag="VFT03_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU4847460.1"
                     /translation="MTTPQDSTKTTERDPRPSEGSPDEPRRAPGAGEQSTMPPRRTWL
                     WFALILLANVLVVRFLIPGPEAPLEVPYTFFKEQVRAGNVEAIYSQGDSVEGRFEEPV
                     TYPPKGEGGSEPGSTPSRTSKHFTTVLPSFVDPGLEELLIDNGVEISAKPIQEGGNPL
                     LTLLFAFGPALLLIGGYIWLYRRAQQQGGGVGGALTGVGRSRARRYDQESETRVTFDD
                     VAGIDEAENELVEIVDFLKDPEKYTRLGGAAPKGVLLVGAPGTGKTLLARAVAGEAGV
                     PFFSLSASEFVELFVGVGASRVRDLFKQARENAPAIIFIDELDSIGRARGQAAIGGSS
                     EQEQTLNQILTEMDGFSSKEGVIVLAATNQPDVLDKALLRPGRFDRRVVVNLPDKVGR
                     EAILKVHTRSVPLADDVDLGVLAASTPGLSGADLRNLVNEAALLAARREQDEVRQKDF
                     LDALEKIVLGPERPLLMSPEERERVAYHEGGHAILGLVVPGADPVNRVTIVPRGQALG
                     VTYQRPEDDRYNYPEAYLRARIVGALGGRAAEEIVYGTKTTGAESDIEQATDLARRMV
                     TRWGMSEKLGTVQLAPRENVYLGGPGGYGGEKPFSEETARAIDEEVSRIIDESHEEAR
                     RLLTKYRRELDALAQALLERETLDEQEILEVTGLPPAPPLENTKIAASSSNSRRDGG"
     gene            complement(2312..3514)
                     /locus_tag="VFT03_04630"
     CDS             complement(2312..3514)
                     /locus_tag="VFT03_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEU4847461.1"
                     /translation="MTSQAGEEAAVRPFSIDVPEEELAELRQRITATRWPSKELVEDR
                     SQGVQLATLQELARYWATAYDWRMVEARLNALPQFTTEIDGVEIHFIHVRSRHENALP
                     LIMTHGWPGSVIELLETVGPLTDPTAHGGNPEDAFHLVLPSLPGYGFSGEPTELGWES
                     GRIARAWAELMDRLDYTRYVAQGGDVGAAVTDAMGRQAPEGLVGIHINLLVASLGLED
                     QLPAESEQERAAHEAVNTFTTDGFGYFLEQSTRPQTIGYSLLDSPVALAAWLLDHDTD
                     SYYKISRAFVDGDPVGNLTRDNIVDNITLYWLTGTSASAARWYWETGRAQAAAQAAGQ
                     APPAVSVPVGFTTFPGEIFAAPRSWVEVVYPELSYFNEVDRGGHFAAWEEPELFSEEI
                     RAAFRSLR"
     gene            3882..4070
                     /locus_tag="VFT03_04635"
     CDS             3882..4070
                     /locus_tag="VFT03_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847462.1"
                     /translation="MVGWRYKPGVAEQRKAETAAARAAHGGKGKAGTEIRGVHFWVNL
                     ARKDKGVEPSAQILPRYR"
     gene            complement(4125..4388)
                     /locus_tag="VFT03_04640"
     CDS             complement(4125..4388)
                     /locus_tag="VFT03_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847463.1"
                     /translation="MKRAIFLATAALLVMLVLAPVALAQDTTQMGAETQMGGATKAGG
                     ATQAGGGTAGKPLPPSGGPEILLPAAALLLGSGVLTYAVLRRR"
BASE COUNT          792 a         1518 c         1444 g          866 t
ORIGIN      
        1 acgaacatgg cagccatcgc gcagaagatc agcagccgcg cagagatcgc ctccgctgcc
       61 gtgagatcgc ctatccaccg tctcgccggg agtttgagct cgaggctgca atcttcgtgt
      121 tctccagcgg cggcgcgggg gggagccccg tgacctccag gatctcctgc tcgtccagcg
      181 tctcccgctc cagcagagct tgggcgagcg cgtcgagctc tcgacggtat ttcgtgagta
      241 accgccgggc ttcttcgtgg ctctcatcga tgatgcgcga cacctcttcg tctatggcgc
      301 gggcggtctc ctcgctgaaa ggtttctctc cgccgtaacc gcccggtccg ccaaggtaga
      361 cgttctcgcg cggagctagc tgcaccgtcc ccagcttctc gctcatcccc cagcgggtga
      421 ccatcctcct cgcaaggtcg gtggcttgct cgatgtcgct ttcggcccca gtggtcttcg
      481 tgccgtagac gatctcctcc gctgcccttc cccccagggc gccgacgatc ctcgctctca
      541 agtaagcttc cgggtagttg tagcggtcgt cctcggggcg ctggtaggtg acccctaaag
      601 cctgcccccg cggtacgatc gtcacccggt tgacggggtc ggctccgggc acgacgaggc
      661 ccaggatggc gtgaccgccc tcgtggtagg cgacgcgctc gcgctcctcc ggactcatca
      721 agagcgggcg ctcgggtccg agcacgatct tctccaaagc gtccaggaag tccttctggc
      781 ggacctcgtc ctgctcgcga cgcgccgcca ggagcgccgc ctcatttacg aggttcctca
      841 ggtcggcgcc cgagagccct ggtgtcgaag ccgcgagcac ccccaggtcc acgtcgtccg
      901 cgagcggcac gcttcttgtg tgtaccttga ggatagcctc gcggccaacc ttgtccggga
      961 ggttgaccac cacgcgccgg tcgaagcgcc cgggcctgag aagagccttg tcgagcacgt
     1021 cgggctggtt ggtcgcggcc aaaacgatca caccctcctt actggagaag ccgtccatct
     1081 cggtgaggat ctggttgagg gtctgctcct gctcgctcga tcccccgata gccgcctggc
     1141 cgcgggcgcg tcctatggag tcaagctcgt cgatgaagat gatcgccggg gcgttctcgc
     1201 gggcctgctt gaagaggtcc ctaacgcggc ttgcgcctac ccccacgaac aactcgacga
     1261 actcggaggc gctcaaggag aagaacggaa cccccgcctc tcccgcgacc gcccgcgcca
     1321 ggagcgtctt gcccgttccc ggcgctccca ccagcagcac gcccttcggg gccgctccgc
     1381 ccaggcgggt gtacttctcg gggtccttga ggaagtccac gatctcgacc agctcattct
     1441 cggcctcgtc tatgccggcg acgtcgtcga acgtgacccg cgtctcgctc tcctggtcgt
     1501 agcgcctggc gcggctccgg ccgacgcccg tgagggcgcc gcccacgccg ccaccttgct
     1561 gctgcgcgcg cctgtagagc cagatgtagc ccccgatcag gagcagcgca ggcccgaatg
     1621 cgaagagcag cgtcagcaac gggttaccgc cctcttgtat cggcttggcg ctgatctcga
     1681 ccccgttgtc gatcaagagc tcctcgagcc ctgggtcaac gaaggaaggt agcacggtgg
     1741 tgaagtgttt ggaggtgcgc gaaggcgtcg agccgggctc cgagcctccc tctcctttag
     1801 gcgggtaggt taccggctcc tcgaagcggc cctcgacgga gtccccctgg ctgtagatcg
     1861 cctcgacgtt acccgccctg acctgctcct tgaagaaggt gtagggcacc tcgaggggcg
     1921 cttcggggcc tggaatgagg aacctgacaa cgaggacgtt cgcgagcagt atgagcgcga
     1981 accacagcca tgtccttctt ggcggcatgg tgctctgttc ccctgctccg ggcgcgcgcc
     2041 gcggttcgtc cggagaaccc tcggaaggac ggggatccct ctcggtcgtc ttggtgctgt
     2101 cttgtggcgt ggtcatcgga cacctccttc ggcgtgccgg gtggggatgg taatgacggc
     2161 tgtttctaac acctggtaac gctctggcag cccatcttca acgtcatcac tattcccact
     2221 tccatagagc gatgttggga tggtgatgat tggttttcgg aggtatgcca gattcgacaa
     2281 agctgggaat cgcgccaccc cttctctgca gctagcgcag tgatctgaac gccgcgcgga
     2341 tctcctcgga gaacagctcc ggctcttccc aggccgcgaa gtggccaccc ctgtcgacct
     2401 cgttgaagta gctgagctcg gggtagacca cctcgaccca gctgcgcgga gcagcgaaga
     2461 tctcgccggg aaaagtcgtg aagccgaccg gaaccgagac cgccggaggc gcctggccgg
     2521 ccgcctgggc tgcagcctga gcccgtccgg tctcccagta ccaccgggcg gccgaggcgc
     2581 tggtacccgt cagccagtag agcgtgatgt tgtcgacgat gttgtctcgg gtgagattgc
     2641 ccacagggtc gccgtcgacg aacgcgcggg agatcttgta gtagctgtcc gtgtcgtggt
     2701 cgagcaacca ggctgcaagg gcgacgggtg aatccagcag ggagtagccg atcgtctgcg
     2761 gccgggtgga ctgctccagg aagtagccga agccgtccgt cgtgaacgtg ttgaccgcct
     2821 cgtgcgccgc gcgttcctgc tcggactccg ccggcagttg gtcctcgaga cccagcgacg
     2881 ccacgagcaa gttgatgtgg atgcctacca gcccctcggg tgcttggcgg cccatcgcgt
     2941 ccgtgacggc ggcgcccacg tcgcccccct gggcgacgta gcgcgtgtag tcgaggcggt
     3001 ccatcagctc cgcccacgcg cgtgcgatgc ggccggactc ccagccgagc tcggtcggct
     3061 cgcccgagaa gccgtagcca ggcaaggacg gcagcaccag gtggaatgcg tcttcggggt
     3121 tgccgccgtg agcggtcggg tcggtgagcg ggccgaccgt ctccagcagc tcgatgaccg
     3181 agccgggcca gccatgcgtc atgatcagcg gcaacgcgtt ctcatgccgc gacctgacgt
     3241 ggatgaagtg gatctcgact ccgtcgatct cggtcgtgaa ctgcggcagc gcgttcagtc
     3301 tcgcctccac catgcgccaa tcgtaagccg tcgcccaata gcgcgcgagc tcctgcagcg
     3361 tcgccaactg cacgccctgc gaccgatctt cgacgagctc cttgctgggc cagcgtgtcg
     3421 cagtgatgcg ttggcgcagc tcagcgagct cctcttctgg aacgtcgatg ctgaaggggc
     3481 gtactgcagc ctcttctcca gcttgactgg tcatagtggt ctcctttccc tggattacac
     3541 atcaatccat gcaaacccta cccatgaagt gaacatcagt caatgggata tccgccctag
     3601 tcccgtatgt atcaagagag agcgtcttcc aacgactgag caggtgcttt tccagggtga
     3661 gttttttgcg tgtggcagtg ggaagccgag acgcaaaaag tccctcaagg ccattgtaga
     3721 aagcctaacc gatgcgctct gttagacggc caaatgacta aagtcgagct ctaggcactc
     3781 gggggaatcg ccccttttcg gacgcctcag acctcactgg ctggcattat gggctagcgt
     3841 gaaaggcggg gtagcaacgc agggaggcgg ccttattcgt cgtggtaggc tggaggtaca
     3901 agcctggcgt agcagagcag cgtaaggccg agaccgcagc ggctcgcgca gcgcatggtg
     3961 ggaagggcaa ggccggaacc gagatacgcg gcgtccactt ctgggtgaac ttggcgcgca
     4021 aggacaaggg agtcgagccg agcgcgcaga tcctcccccg ctacagataa taaaatcccg
     4081 cgccccctgt acggggacac gggacaccta gattctggtc gatgctacct gcggcgcaga
     4141 acggcgtacg tcagaacgcc cgagccgagc agcaacgcgg ccgccggcag gaggatctcc
     4201 gggccaccgc tcggcggcag cggcttaccc gccgtcccgc cacccgcctg ggtcgccccg
     4261 cccgccttgg tcgccccacc catctgggtt tccgcgccca tctgggtggt gtcctgcgcc
     4321 aacgcaacgg gcgccaggac gagcatgacc aggagagcgg ccgtagccag gaatatcgcc
     4381 ctcttcatcc agtcctcctc ttctacgtcg ttttccaatc agcctgcatc tgcccctcct
     4441 ccagcagaga aggtacccac agtgtacaga acctaaacgc aaaagtctcc attattttaa
     4501 taatttttaa tgaaaatatc gcaataagac cgttgaaatt gccgacacgg gctggaagcg
     4561 ggggtagtag gattcgaacc tacgggtgtt ttaccacctc tggttttcaa gaccagcgca
//
LOCUS       DASTUV010000229         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_832438, whole genome shotgun sequence.
ACCESSION   DASTUV010000229 DASTUV010000000
VERSION     DASTUV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832438"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFT03_04645"
     CDS             <1..243
                     /locus_tag="VFT03_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847464.1"
                     /translation="TEEVGAVTGDLELFTRPSAEGSVDVLVRYAGADEWYTVSGSPAQ
                     MSGGDLRATHEALLARLTTPGPYEGDNEMPVDLLGR"
     gene            complement(301..1206)
                     /locus_tag="VFT03_04650"
     CDS             complement(301..1206)
                     /locus_tag="VFT03_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4847465.1"
                     /translation="MGSDVWTAERVPGEVELAIWRSGSGEDPIVCLHGITAQHRAFNT
                     SARHLAPSRSLVGVDLRGRGDSDKPGSGYGLEAHARDVIRVLDNLDLEDAVICGHSMG
                     GFVALRTALSYPGRARALVLLDSGWPRMEADPEEMSEEQKQEAAAIEEGLERAFRRLD
                     MTFESPEDYLDFWFPNQDLAPGDLPADIADYYLYDLERVEGGYRPKASRAAAEEDSSS
                     VSSAAPTLEELRGVGCPVALVRASQGFFPGADPLVSDDVRDAMAGALDLRSDNRIEGA
                     NHYTMLWPEYVPKWAGILRDDTWCR"
     gene            1282..2286
                     /locus_tag="VFT03_04655"
     CDS             1282..2286
                     /locus_tag="VFT03_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU4847466.1"
                     /translation="MDYYEDILDAVGRTPLVRLGRIEKGEGIRAHLLAKVEYLNPGGS
                     VKDRPAVKMLEVAEKEGLLKPGGTVVEPTSGNTGAGLALAAAVKGYHCICVMPDKTSK
                     EKQYLLRALGAEVIVTPTGLTPDDPESYYAVAERLAREMPGGFKPGQYENPANSLAHY
                     ETTGPEVWEQTDGRITHFVAGMGTCGTITGNGHYLKEKNPQIKIIGADPEGSIFSDPS
                     NVHTYAVEGVGEDFYPGNYDPEVVDEVIQVDDRESFLMTRQLMREEGLFVGGSCGMAV
                     VAAVRAARGLPEDAVMVVLLPDTGRNYVSKVFDDQWVLENSEATSEDLGRPYRVFEEA
                     "
     gene            2287..>2781
                     /locus_tag="VFT03_04660"
     CDS             2287..>2781
                     /locus_tag="VFT03_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU4847467.1"
                     /translation="MRFATKAIHVGQEPDPTTGSTIVPIYQTSTYTQEAPGQHKGYEY
                     SRTANPTRTALEECVASLEGGEYGLAFASGLAATVATMSLLSPGDHVVAGDDLYGGTY
                     RLFDKVLARSGGLEFTYADTTHPEAVGKALRPETKLLWIETPTNPLLTISDIEELSET
                     ARERG"
BASE COUNT          531 a          878 c          935 g          437 t
ORIGIN      
        1 accgaggaag tcggcgccgt aacgggcgat ctggaactct tcacccgccc gtccgctgaa
       61 gggtcagtgg acgtcctggt caggtacgcc ggcgcagatg agtggtacac cgtatcgggc
      121 tcgcccgcgc agatgagcgg aggggacctt cgcgctacgc acgaggcgct gctggcgcgg
      181 ctcacgacac caggtcccta tgaaggcgac aacgagatgc ccgtcgacct gcttggacgg
      241 tagcatccgt tcgggagcaa gtgagaagat ctacgagaac tattcctgaa acgtatttcc
      301 tcaacggcac caggtatcgt cacggaggat gccggcccac ttcggtacgt actccggcca
      361 cagcatcgtg tagtggttcg ccccctctat tcggttgtcc gagcggaggt cgagcgcccc
      421 ggccatcgcg tcccggacgt cgtcggagac tagggggtcg gcgccgggga aaaatccttg
      481 cgaggcccta accagggcaa ccgggcaccc gacgccgcgc agttcctcca gggttggggc
      541 tgccgaggag accgaggacg agtcctcctc ggcggcggca cgggaggcct tgggcctgta
      601 gccaccttcg actctttcga gatcgtagag gtagtagtcg gcgatgtcgg ccgggaggtc
      661 gcccggcgca aggtcctggt tcgggaacca gaagtcaagg taatcctcag ggctctcgaa
      721 ggtcatgtct agacgcctga aggcccgctc gagaccttcc tcgatggccg cagcctcttg
      781 cttctgctct tcggacatct cttcggggtc ggcttccatg cggggccagc cgctgtcgag
      841 aaggacgaga gcgcgggcgc gaccagggta ggagagtgcg gtccggaggg cgacgaagcc
      901 gcccatggag tggccgcaga tcacggcgtc ttccagatca aggttgtcga gaacgcggat
      961 cacgtcccga gcgtgcgcct cgagcccgta gcccgatccg ggcttgtccg agtctccccg
     1021 gccccgcagg tctacgccga cgagggaacg cgatggtgcg agatgccttg ccgaggtatt
     1081 gaacgcgcgg tgctgggcgg tgatgccgtg caggcagacg atagggtcct cgccgctccc
     1141 ggagcgccag atcgccagct cgacctctcc gggcaccctc tccgcggtcc agacgtcact
     1201 ccccatgtcc gagcagtata cgcgagggcg agttttcgcg aggacgcgtg atggtaatct
     1261 acggatcggg aggtctcgaa catggactac tacgaggaca ttttggacgc cgtcgggaga
     1321 acgcccctcg tccggctcgg gcgcatcgag aagggcgagg gcattcgcgc ccacctgctg
     1381 gcgaaggtgg agtacctgaa tccgggcggc tccgtgaagg acaggccggc cgtgaagatg
     1441 ctcgaggtcg cagagaagga ggggctgcta aagcccggcg gtaccgtcgt cgagccgacg
     1501 agcggcaaca cgggagccgg tctagcactc gcggcggcag tcaagggcta tcactgcatc
     1561 tgcgtgatgc cggacaagac gagcaaggag aagcagtatc ttctaagggc tctaggcgcc
     1621 gaggtgatcg taactcccac cggtcttacc ccagacgacc cggagagcta ctacgctgtc
     1681 gcggagaggc tggcgcgcga gatgccgggc ggtttcaaac cgggccagta cgagaacccg
     1741 gcgaactctc tggcccacta tgagacgacg gggcccgagg tgtgggagca gacggacggg
     1801 cgcatcaccc acttcgtcgc cgggatgggc acctgcggca ccatcaccgg caacggacac
     1861 tacctcaagg agaagaatcc acagataaag ataatcgggg cggacccgga gggctctatc
     1921 ttctccgacc cgagcaacgt gcacacgtac gctgtcgaag gcgtcggcga ggacttctac
     1981 ccaggaaact acgaccccga ggtggtcgac gaggtgatcc aggtcgacga ccgcgagtcc
     2041 ttcctcatga cgcggcagct catgcgggag gagggccttt tcgtcggggg ctcgtgcggt
     2101 atggccgtcg tcgccgcggt ccgggcggcc agagggttgc cggaggacgc cgtgatggtc
     2161 gtgttgctgc ccgacacagg gcgcaactac gtctccaagg tgttcgacga tcagtgggtc
     2221 ctggagaact ccgaggcgac ctcggaggac ctgggcagac cttaccgcgt cttcgaggag
     2281 gcatagatga ggttcgccac gaaggccata cacgtaggcc aggagccgga cccgacgacc
     2341 ggctccacga tagtcccgat ctaccagaca tccacctaca ctcaggaagc gccgggccag
     2401 cacaagggct acgagtacag ccgtaccgcc aaccctaccc gaacagccct cgaagagtgc
     2461 gtagcttccc tcgaaggcgg cgagtatggc ctcgcctttg cctccgggct cgccgcgacc
     2521 gtcgcaacga tgagcctcct ctctcccggg gaccacgtgg tggccggcga cgacctctac
     2581 ggcggcacct accgcctctt cgacaaggtg ctcgcccgca gcggcggcct cgagttcaca
     2641 tacgccgaca ccacgcaccc ggaggcagtg ggaaaggccc tcaggcccga gacgaagctg
     2701 ctctggatcg agacgcccac caacccgttg ctcaccatct cggacataga ggagctctcg
     2761 gagacggcgc gcgagcgggg g
//
LOCUS       DASTUV010000230         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1115248, whole genome shotgun sequence.
ACCESSION   DASTUV010000230 DASTUV010000000
VERSION     DASTUV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115248"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="VFT03_04665"
     CDS             complement(<1..90)
                     /locus_tag="VFT03_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU4847468.1"
                     /translation="MKFKPLGDRALVKLVEREETTPSGIVLPDT"
     gene            complement(208..519)
                     /gene="trxA"
                     /locus_tag="VFT03_04670"
     CDS             complement(208..519)
                     /gene="trxA"
                     /locus_tag="VFT03_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4847469.1"
                     /translation="MAVAEVTDATFESDVLKSEKPVIVDFWAPWCVPCRRVSPILEEM
                     SEERDDVRFYKLNVDDNPSTAMNYSISSIPTIARFEDGQVTQQAIGALPRNQLSAQLG
                     L"
     gene            complement(646..1605)
                     /locus_tag="VFT03_04675"
     CDS             complement(646..1605)
                     /locus_tag="VFT03_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEU4847470.1"
                     /translation="MILTVTLNAAVDRTLVVPSLTLGHRHRAPESVTLAGGKGINVAR
                     SLRTLGVPVLATGFVGGRNGDAIRDGLSAAAIPFDLVEIEGYSRTSTAIIDPMAGTQT
                     EINEYGPEVSPSEFREFTRRLDHLMEYATAIVFAGSLPPNLDESFFVGLVQRSRERGL
                     YTVVDSTPSVLRAVLKGRPSLVTPNQHEAESVVGFDFIEEGDFIRSLSRLVELGALGA
                     CITSPEGHSYLISDAGIVSGRAPKVETISTIGSGDAYLAGLLAGLLHRNLEPVDAVRL
                     AAGCAAANAETLGAGLFEARRAEELAESVQIDLLSRSFEESRA"
BASE COUNT          354 a          688 c          641 g          388 t
ORIGIN      
        1 cgtgtcgggc aagactatgc cggagggtgt agtctcctca cgctccacga gcttaaccag
       61 agctctgtcg ccgagtggct tgaacttcat cccgagtacc tccttggaac ttcggactta
      121 cacggttaac ggcgcgatcc cgttagaccc atccggcctc cgggatcacc cgcccgagat
      181 tgtaaccttg ggcgttactt gctgcaatta caggccgagc tgtgcggaga gctggttcct
      241 tggcagagcg ccgatggcct gctgggtgac ctggccgtcc tcgaagcgag cgatggtagg
      301 gatgctggag atgctgtagt tcatcgcggt cgaggggttg tcgtctacgt tgagcttgta
      361 gaaccgcacg tcgtcgcgct cctcggacat ctcctcgagt attggggaga cgcgccggca
      421 cggcacgcac cacggcgccc agaagtccac tattaccggc ttctccgact tgagaacgtc
      481 gctctcgaac gtcgcgtcag ttacttcggc aaccgccata aatgcacaac ctccgtcgct
      541 ctggacaacc ggcttttgac ccggctattc tagcacgcaa ggcctcaggt tcagccgtac
      601 gtgagggcgg gcagctcgcg aattgccttt acgcgaaaac tgcctctacg cccggctctc
      661 ctcgaagctg cgggacagga gatctatctg cacggactcg gctagctcct cggcccggcg
      721 cgcctcgaac agcccggcgc ctagagtctc ggcgttagcg gcggcgcacc cggccgccag
      781 gcgcacggcg tccacgggct ccaggttccg gtgcaggagc cctgcgagga gtcccgcgag
      841 gtaggcatcg ccgctcccga tagtcgagat ggtctccacc ttgggcgcgc gacccgaaac
      901 gatgccggcg tcggagatca ggtacgagtg cccttcgggc gacgtgatgc acgcccccag
      961 agcgccgagc tccaccaggc gggagaggct gcgaatgaag tcgccctcct cgataaaatc
     1021 gaagccaacg acgctctcgg cctcgtgttg gttcggggta acgagcgagg gacgcccctt
     1081 gagcactgcc cgcaggacgg acggcgtcga gtcgacgacc gtgtagagac cgcgctcccg
     1141 cgagcgctgc acgaggccga cgaagaagct ctcgtccaga ttcggcggca ggctcccggc
     1201 gaagacgatc gcggtcgcgt actccatcag gtgatctagg cgccgggtga actccctgaa
     1261 ctccgaaggg ctgacctcgg gtccgtactc gttgatctcc gtctgggtcc cggccatcgg
     1321 gtctatgatg gccgtcgagg tccgcgagta tccctctatc tcgaccaagt cgaagggtat
     1381 ggccgcggca gacagaccgt cgcggatcgc gtcgccgttg cgcccgccga cgaagcccgt
     1441 ggcgagaacc ggtacgccaa gggttctcag actgcgggcg acgtttatgc ccttgccgcc
     1501 cgcgagcgtg acgctctcgg gcgcgcggtg gcggtgcccg agggtcaggc tggggacgac
     1561 cagcgtgcgg tctacggcgg cgttcagggt gacggtcagg atcatcggca acgtgcctcc
     1621 tctacgctac tcctccaggc gaaggtcccc cgcctgtata ccacagccca gcaccgtact
     1681 cctctcgccg ctcgggtaag aggtctccgg tgtcccacat tgggccaacc tcctgtgtat
     1741 cggccccgaa tcaagaaacc gtcgaacagc tctgcggctc tccgcctcat tccggcagca
     1801 cgggcgcgta gcgctcgagc gtcatcgcag ggtctgcgtg acggagtata tccgtcacga
     1861 gcctcgattt cagcacgctc cagccttcgg cttccgcttg tcagcacgct ccagccttcg
     1921 gcttccgctt ctcagcacgc ttcggccctt ttagggcctc gctctcagca cgccgcctgg
     1981 cttcgccagg cggcttctca gattctcttt gcgctgacag cggtactcat aaaggttgat
     2041 cctgaggcca ataaccagcg tgagcgaacc a
//
LOCUS       DASTUV010000231         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1722626, whole genome shotgun sequence.
ACCESSION   DASTUV010000231 DASTUV010000000
VERSION     DASTUV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722626"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VFT03_04680"
     CDS             <1..1245
                     /locus_tag="VFT03_04680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU4847471.1"
                     /translation="LKSEGDEVSEGDPIAEIETDKVTLELEAEDSGTLAQLIADEGQD
                     IPVGEAIAFIAGEGEEVPERAAGGGAEEAEEEGGEGEAQAQTATETEAPEEEGGEEAA
                     AQADGRTDGHFRASPIVRRLAQENNLDLSRIEGSGPAGRIVERDVRAAMESGTAQKTD
                     GKAEVEAPEREAAPQVAAAPEPTRAPGTELVEPSRMRRVIGERMTQSKQQVPHYYATV
                     EVEMNDLLALRKQLNEQLEEQGIKLSINDFVMKAVALALKDFPNLNALWTSEGIELHQ
                     QVDLAMAVALEEGLITPVIRDAANKTLSAISAASKDLAKRAREGALKPEEYQGGTVTV
                     SNMGMFGVESFAAIINPPQAAIVAVSSIMQRAMFKDDEVVPVSIMKLTLSADHRISNG
                     AEGGRYLAEVKRLLERPMTLVV"
     gene            complement(1388..2167)
                     /locus_tag="VFT03_04685"
     CDS             complement(1388..2167)
                     /locus_tag="VFT03_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4847472.1"
                     /translation="METLRNLFDPLFNLLGATLTTFHGWGVPWWLAIIMLTAVVRTLL
                     FPLTVRQAKSMRRMQELKPDMDEIRARYKDDPKKQQQEMMKLYGERKVNPLGGCLPLV
                     VQLPIFIALYYTIKQFDKLESFVHGGLFWFQDLTVADPYFILPIAYVLTMMASQELTI
                     RRTAPQQKQLMRIMPIAFGIFLVRFPAALFVYWISSNTITFVQNYLIYHRSPKKDPAE
                     DLATPDEEKTRTEPDPQPSQSASGNRPARKRKRRKKRKKGK"
     gene            complement(2242..>2833)
                     /locus_tag="VFT03_04690"
     CDS             complement(2242..>2833)
                     /locus_tag="VFT03_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847473.1"
                     /translation="IRQTFRINSGSAATRPSFPRPKLIPRTPAGGLAYRKVGLRAASP
                     PEVLERPFEAFDECYYPVVSESGTHPARRSRFDGLQQTALFAIWLLLVDVSPQQDVYA
                     NPDYGLSLLAALGTAGCVGYLLTAIFRLYRAPFGSQGVLSRSSSVEAFTSDGRHGGEN
                     WPLLVALLFGSFLVLFSGLGGWVDAYPRVTRPALPA"
BASE COUNT          568 a          834 c          973 g          458 t
ORIGIN      
        1 ctcaagagcg agggtgacga ggtttccgag ggcgacccga tagccgagat cgagaccgac
       61 aaggtgacgc tggagctcga agccgaagac tccggcacgc tcgcccagct catcgccgac
      121 gagggccagg atatccccgt gggcgaggcg atagccttta tcgccggcga gggcgaggag
      181 gtgccggagc gggccgccgg cggcggagcc gaagaagccg aggaagaggg cggcgagggc
      241 gaagcccagg cccagaccgc cacggagacc gaggcgcccg aggaagaagg tggagaggag
      301 gctgccgctc aggccgacgg ccgtacagac ggccacttcc gagcctctcc gatagtgaga
      361 cgcctggccc aggagaacaa cctggacctc tccaggatag agggttcggg accggccggt
      421 cgcatcgtcg agcgcgacgt gcgggcggca atggagagcg gcacagccca gaagaccgac
      481 gggaaggccg aagtcgaggc tccagaacgg gaggccgcac cgcaggtggc tgccgcgccc
      541 gaaccgaccc gggcgccggg gaccgagctc gtcgaaccga gccggatgcg ccgggtcatc
      601 ggcgagcgga tgacgcagtc caagcagcag gtcccccact actacgcgac cgtcgaggtc
      661 gagatgaacg acctgctggc gctgcgcaag cagctcaacg agcagctcga ggagcagggg
      721 atcaagctct cgatcaacga cttcgtgatg aaggccgtcg cgctggccct caaggacttc
      781 ccgaacctca acgctctgtg gacctccgag ggtatcgagc tgcatcagca ggtagacctg
      841 gcgatggcgg tcgccctcga ggagggcctc ataacccccg tcatcaggga cgcggccaac
      901 aagaccctct ccgccatctc cgcggcctcg aaggacctgg ccaagcgcgc ccgcgaggga
      961 gcgttgaagc ccgaggagta ccaggggggc acggtcaccg tctccaacat gggcatgttc
     1021 ggcgtcgaga gcttcgccgc catcatcaac ccgccgcagg ccgcgatagt cgccgtgagc
     1081 agcatcatgc agcgcgccat gttcaaggat gacgaagtcg tgccggtgag cataatgaag
     1141 ctcaccctct ctgcggacca caggatctcc aacggcgccg aaggaggccg gtacctggcc
     1201 gaggtcaagc gcctcctcga gcgcccgatg acgctcgtgg tctgaagcca gtcagccctt
     1261 caaccgacag tacggcgccc ccggctctcg ccgggggcgt tttttactgt ttccgagaaa
     1321 tgatgatcca ggcaagaacc cgggagagcc caagcctcca gaacaaaagc tgaagtgctc
     1381 acctccttca cttccctttc ttgcgcttct ttcttctttt tctcttgcga gccggtctgt
     1441 tcccgctggc gctctgtgaa ggttgaggat cgggctccgt cctggtcttc tcttcgtccg
     1501 gggtcgcgag gtcttcggca ggatccttct tgggcgagcg atggtagatg aggtagttct
     1561 ggacgaaggt tatggtgttc gaagagatcc agtagacgaa gagcgccgcc ggaaagcgca
     1621 cgaggaagat gccgaaggcg atgggcatga tgcgcatgag ctgcttctgc tggggagcgg
     1681 tgcgcctgat ggtgagctcc tgggaggcca tcatggtgag gacgtaggcg atagggagga
     1741 tgaagtaggg gtctgcgacg gtgaggtcct gaaaccagaa caggccgccg tggacgaagc
     1801 tctcgagctt gtcgaactgc tttatcgtgt agtagagcgc gatgaagata gggagctgga
     1861 cgacgagggg cagacaacct cccagagggt tgaccttccg ctcgccgtag agcttcatca
     1921 tttcctgttg ttgtttcttc gggtcgtctt tgtacctggc gcggatctca tccatgtcgg
     1981 gcttgagctc ctgcatcctg cgcatgctct tggcctgccg cacggtcagc gggaagagta
     2041 aggttcgtac cacggcggtg agcattatga tggcgagcca ccatgggacc ccccaaccat
     2101 ggaaggtcgt cagcgttgcg ccgagcaggt tgaagagcgg gtcgaagagg tttctcaggg
     2161 tttccatcgt gcgcgttcct tctagtgtcg cctgggagct gtcgggcatg cgcgcggtat
     2221 gccccgctcc ttcgccggtc gctaggctgg caaggcgggg cgcgtcacgc gaggataggc
     2281 atcgacccaa ccgccgaggc cggagaagag caccaggaag gacccgaaaa gtagcgcgac
     2341 aagcaagggc cagttttctc cgccgtggcg gccgtcgctc gtgaacgcct cgaccgaaga
     2401 ggagcgtgag agcactccct gagagccgaa cggagcgcgg tacaggcgga agattgcggt
     2461 aaggaggtag ccgacacacc ctgcggtgcc gagggcagcg agcagcgaga ggccgtagtc
     2521 cgggttcgcg tacacgtctt gctggggcga aacgtcgacc aggagcagcc agatagcaaa
     2581 cagcgcagtc tgctgcagac cgtcaaaacg gctgcgccgc gcgggatgag ttccggactc
     2641 gctcacgacg ggataataac actcatcgaa agcttcgaag ggtctctcca gaacctccgg
     2701 cggggaggct gcgcggaggc cgaccttgcg ataagctaaa ccccccgccg gggtgcgggg
     2761 tatcagtttg ggtcgaggaa aggagggtct cgtggcggca gagccggagt tgatccgaaa
     2821 cgtttgcctg atc
//
LOCUS       DASTUV010000232         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_538981, whole genome shotgun sequence.
ACCESSION   DASTUV010000232 DASTUV010000000
VERSION     DASTUV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538981"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="dhaK"
                     /locus_tag="VFT03_04695"
     CDS             <1..966
                     /gene="dhaK"
                     /locus_tag="VFT03_04695"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU4847474.1"
                     /translation="EDMLRGMELAHGGLIRVIPESNVVVRADAPVEGKVALVSGGGSG
                     HEPTHGGFVGPGMLDAACAGAVFTSPTPDQMFEASKAVDGGAGVFYVVKNYTGDVLNF
                     EMAGELAEAEGVQTDYVVTNDDVAVEDSTYTSGRRGIAGTIFVHKICGAAADDGKDLA
                     GIKELAERVNSNVRSMGMALTSCTPPESGEPIFDIGEDEMEIGIGIHGEPGIERKKIE
                     PADQIVDELLARILGDSVDFSGSEVAVMVNGMGGTPLMELYVAYAHVAEVLDREGIQV
                     WRMPYVGDYMTSLEMAGFSITLLKLDDEMKGYLAAPCDVAAGRPF"
     gene            983..1690
                     /locus_tag="VFT03_04700"
     CDS             983..1690
                     /locus_tag="VFT03_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEU4847475.1"
                     /translation="MEAYLGEFIGTMLLIILGDGVVAGVLLRNSKAENSGWIVITLGW
                     GMAVAVAVYCTVNISGAFINPAVVLGFAVTGDLEWALVPGYIIAEFLGAFVGAVIVWL
                     AYLPHWEETADPGLKLGVFSTAPAIYNTPANIICEIIGTFVLVFGVFGILANDLATGL
                     FPLLVGFLVLGIGLSLGGPTGYAINPARDLGPRIAHAVLPIAGKGGSDWGYAWIPVVA
                     PIIGGILGALAFTLLFG"
     gene            1842..>2731
                     /locus_tag="VFT03_04705"
     CDS             1842..>2731
                     /locus_tag="VFT03_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU4847476.1"
                     /translation="MSPTEHVPRGHLSGRQTAEARPASIAEAFDSNALSAPEKLCLRF
                     EDEDWSYGRLRGRAGAFAAALGSWGLQPGDRIALFLGNHPDFLAAYLGAHLAGAVVVP
                     VNRSYRRAELRHIFGDAGVRLCVTDGEGRPELERVRGDLPALERVVERGKDLDIFLGE
                     GGSGELAMPRGEDLALIAYTSGTTGRSKGAMLLHRNLLGNAEAICAAWKWTAEDHLLL
                     TLPLFHSHGLMVGAHGTLLTGASAELRRGFDAAGVYDALLSGNFTMFFGVPTMYTRLL
                     REAERRSERPKPLRLYVSGSA"
BASE COUNT          460 a          899 c          918 g          454 t
ORIGIN      
        1 gaggacatgc tcagggggat ggagcttgcc cacggcggcc tgatccgggt catccccgag
       61 tccaacgtcg tcgtaagggc cgacgccccc gtagagggca aggtcgccct cgtctccggc
      121 ggcggctcgg gccacgagcc gacccacggc ggtttcgtcg gacccggtat gctcgacgcc
      181 gcctgcgccg gagccgtatt cacgagcccg actccagacc agatgttcga ggctagcaag
      241 gcagtggacg gtggggccgg tgtcttctac gtcgtcaaga actacacggg cgacgtcctc
      301 aacttcgaga tggccggaga gctcgccgag gccgagggcg tacagaccga ctacgtcgta
      361 accaacgacg acgtggcggt cgaggactcg acgtacacca gcgggcggcg cggcatcgca
      421 ggcaccatct tcgtgcacaa gatctgcggc gcagcggccg acgacggcaa ggacctcgca
      481 ggcataaagg agctcgcgga aagggtcaac tccaacgtgc gctcgatggg gatggccctg
      541 acgagctgca ccccgccaga gagcggcgag cccatcttcg acatcggcga agacgagatg
      601 gagatcggga tcggcatcca cggcgagccc ggcatcgagc gcaagaagat agagccagcc
      661 gaccagatag tggacgagct cctggccagg atcctgggcg attcggtgga tttcagcggc
      721 tccgaggtcg ccgttatggt caacggcatg ggcggcacgc ccctcatgga gctctacgtc
      781 gcctacgccc acgtcgccga ggtcctcgat cgcgaaggca tacaggtctg gcggatgccc
      841 tacgtcgggg actacatgac ctcgctcgag atggctggct tctccatcac gttgctcaag
      901 ctcgacgacg agatgaaggg ctacctcgcg gcgccttgcg acgtggcggc cggacgaccg
      961 ttctagaaag gagaaacaag ggatggaagc atacctaggg gagttcatcg gtacgatgct
     1021 cctcatcatc ctcggtgacg gggtggtcgc cggcgtcctg ctcaggaact ccaaggctga
     1081 gaactcaggc tggatcgtca tcaccctggg gtggggaatg gccgtcgccg tggcggtcta
     1141 ctgcaccgtc aacatcagcg gcgcgttcat caaccccgcc gtcgtcctcg gtttcgccgt
     1201 aaccggagat ctagagtggg cgctggttcc cggctacatc atcgccgagt tcctcggagc
     1261 cttcgtgggc gcggtgatcg tgtggctggc ctatctgccc cactgggaag agaccgctga
     1321 tcccggtctc aagctcggcg tgttctcgac cgcgccggcg atctacaaca ccccggccaa
     1381 catcatctgc gagatcatcg ggaccttcgt gctggtcttt ggcgttttcg gcatactcgc
     1441 aaacgatctc gccacaggtc tctttccgct gctcgtcggg tttcttgtgc tcggcatcgg
     1501 gctgtcgctc ggcggaccaa cgggctacgc cataaacccg gcccgcgacc tcggcccgcg
     1561 catcgcccac gccgtcctcc ccatagcagg caaaggaggc tcggactggg ggtacgcctg
     1621 gatccctgtc gtcgccccca tcatcggcgg cattctcggc gccttggcgt ttactctgct
     1681 cttcggataa cggccggaca cgtgatcggg aggccggcct actgccggcc tcctttttcc
     1741 tggcgggatt gccgggcgcc cgccgcggat gtaaccttgc ccctggcgga ctaccgtcgg
     1801 gcgcgtagga agaagatagg tttgaacgaa gaaaggggcc tatgagccct acggagcatg
     1861 ttccccgcgg ccacctctct ggaaggcaga cggcggaggc gcgaccggcc tctatagccg
     1921 aggccttcga tagcaacgcg ctaagcgcgc cggagaagct ctgcctgcgc ttcgaggacg
     1981 aggactggag ctacgggcgg ctgcgcgggc gcgccggggc tttcgccgca gcgcttggga
     2041 gctggggcct gcaaccaggc gaccggatcg ctctcttcct gggcaaccac cctgacttcc
     2101 tcgcggccta cctcggcgcc catctggctg gtgctgtcgt ggttccggtg aacaggagct
     2161 accggcgggc cgagctgcgc cacatcttcg gcgacgccgg agtgcgactc tgcgtcacgg
     2221 acggggaggg gcggcccgag ctcgagcggg tgcggggaga cctcccggcc ctggagaggg
     2281 tggttgagcg cggcaaggac ctagacatct tcctgggaga aggcgggagc ggcgagctcg
     2341 cgatgccgcg cggcgaggac ctcgccctga tcgcctacac ctcggggacg accggccgga
     2401 gcaagggcgc gatgctgctg caccgcaacc tgctcggcaa cgccgaagcc atctgcgcag
     2461 cctggaagtg gacggccgag gatcacctcc tgctcacctt gcccctgttc cactcgcacg
     2521 gcctcatggt cggcgcgcac ggcacgctcc tcaccggggc tagcgccgag ctgcggcgcg
     2581 gcttcgacgc cgccggggta tacgacgccc tcctctcggg aaacttcacg atgttcttcg
     2641 gcgtgccgac gatgtacacg cggctgctgc gggaggccga gcgccgctcg gagcggccga
     2701 agcccctgag gctgtacgtc tccggcagcg c
//
LOCUS       DASTUV010000233         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_433750, whole genome shotgun sequence.
ACCESSION   DASTUV010000233 DASTUV010000000
VERSION     DASTUV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433750"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFT03_04710"
     CDS             complement(<1..471)
                     /locus_tag="VFT03_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEU4847477.1"
                     /translation="MEAKNYGTADEVVEERDAAQTAEHAEELAGDRSEERFEERGAVA
                     MGTQEERTWSALAHLSVFLNLFTGFLGPVAAGIIWLVYKDRSPQVAFHALQSAAYQVA
                     WIVLLGAGWALTGLLTLILIGFLLIPVMAIATVVPFVHMTYAAYRVSKGDDYRYP"
     gene            585..1094
                     /gene="lspA"
                     /locus_tag="VFT03_04715"
     CDS             585..1094
                     /gene="lspA"
                     /locus_tag="VFT03_04715"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU4847478.1"
                     /translation="MCYDPAVEHTPHHGEDAGVSRKPELLVALVLALAVFVLDQGLKA
                     VVQGAMSPGESIPVIPGFLSITYIRNAGGAFGFFGGSQVLLLIGSAVAVGVVLWMLIA
                     GQRSRLATLACGLILGGAAGNLLDRLTTGEVTDYVHFSFWYVFNAADAAITIGVATLL
                     LSTFRMQEK"
     gene            complement(1239..3260)
                     /locus_tag="VFT03_04720"
     CDS             complement(1239..3260)
                     /locus_tag="VFT03_04720"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU4847479.1"
                     /translation="MAENLVTRILKDHMVSGSLEPGEEVGLRIDQTLLQDATGTMACL
                     QFERMGVERVQVDFAVQYVDHNIIQLDHKNPDDHRFLQAFAAKYGMYYSRPGNGICHY
                     LHVERFARPGATLIGADSHTTSSGALSSIAIGAGGLDVALVMAGQPFETPAPKVVGVE
                     LTGELQDWVTPKDVILELLRRRGVRGGLGRVFEFYGPGVANIDVTGRTTICNMIAELG
                     ATAGIFPSDERTREWLEMQQRPDDFVELSADEGAEYDEHEHIELDRLEPLIAKPHSPG
                     NVVPVSEVAGTKAAQVCIGSSVNSGFQDLAVAAAVLHDETVYPGLVMTVTPGSRQILD
                     SIAQAGVYSDLVRAGARMLEPACGPCVGMGQAPPSEAVSVRTFNRNFPGRSGTLTDQV
                     YLTSPTTAAATALRGVITDPRELGEYPELPEAPANPAVDDRQILEPAPEDEASGIEIP
                     RGPNIKPPPEAPELPDSLEATVTIVVDDDISTGDLAPDGVEVMSFRSNVPEIARFTFR
                     RFDPEYHKKAKEMGPGFIVGGHNYGQGSSREHAALAPLHLGVRAVIAKSFARIHRRNL
                     ISQGILPLRFKDEADYDKFEQGQKWELPDLRKRLENGDEEIPARMQDGTEVTLLAEYS
                     PRERDILLAGGILRQLRQETAPSAAGKHEPQEAEDSDAGTHPSPEVPRG"
     gene            complement(3332..3913)
                     /locus_tag="VFT03_04725"
     CDS             complement(3332..3913)
                     /locus_tag="VFT03_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847480.1"
                     /translation="MSSTTLIIIVVVAVIVLLLLAVYFSMRRRRVQRSERQRERTREE
                     FGSEYERTAEARGSEEEAERELRERRGRVERQVEPLSEESRGRYEEQWDEVERVFVDN
                     PSRSIELADRTVMEVLEERNFVSDPSQSDEETERSLAVMHPDVADDYREARRIRADVV
                     GRFSKESSEEETTEELRQGIQKYRVVYRRIVSG"
BASE COUNT          670 a         1415 c         1334 g          756 t
ORIGIN      
        1 gggatagcgg tagtcgtcgc ccttgctcac cctgtaggcg gcgtaggtca tgtgcacgaa
       61 cggcacgact gtagcgatgg ccatcacagg gatgaggagg aagccgatca ggatcagggt
      121 cagtagcccc gtgagcgccc agccggcgcc gagcagcacg atccacgcca cctggtacgc
      181 agccgactgc agcgcgtgga acgccacctg cggcgagcgg tccttgtaaa ccagccagat
      241 gatacctgcc gccaccggac caaggaaccc ggtaaacagg ttcaggaaca cgctcaggtg
      301 cgcgagggcc gaccacgtcc gctcttcctg cgtgcccatg gcgaccgcgc cacgctcctc
      361 gaagcgctcc tcggagcgat ctcccgcgag ttcttctgca tgctccgcgg tctgtgccgc
      421 gtcacgctcc tcgactacct cgtcggctgt cccgtagttc ttcgcctcca cgatctactc
      481 ctctctgttg ttgacgtcct acggcataaa gttacgctcg aactggcaac ccggcatcgg
      541 accggagacc gaatgttgat ggtcctcagg gaccagatgc ctgcgtgtgc tacgatcctg
      601 ccgtggagca cacgccgcat catggagagg acgccggagt gagccgcaag cccgagttgc
      661 tggtcgccct ggtactcgcg ctggcggtct tcgtcctcga tcagggcctg aaggccgtcg
      721 tgcagggcgc gatgagcccg ggggaaagca tccccgttat accgggcttc ctgagcatta
      781 cctacatcag gaacgccggc ggcgcgttcg gcttctttgg aggaagccag gtgctgctac
      841 tgatcggtag cgccgtggcg gtcggggtcg tcctgtggat gctcatcgcc gggcagcgct
      901 ccaggctggc gactctggcg tgcggcctga tcctgggcgg cgcggccggt aacctcctgg
      961 accgactgac cacgggcgag gtgaccgact acgtccactt ctccttctgg tacgtcttca
     1021 acgccgccga cgccgcaata accatcggcg tagcaaccct gctcctctcc accttcagga
     1081 tgcaagagaa gtaggggtgg ctcgctgggt acgggcccgg cgccatgaga cggtcgagag
     1141 catcgacgtc gagccgcaag cgggcgagga gccactcgcg ctaggcccgc aggactcgct
     1201 cgaccgcgga caaaaccggc gtccaacgag tccgaaggct aacctctcgg cacctcgggg
     1261 ctagggtggg taccggcgtc gctgtcctcc gcctcctggg gctcgtgctt gcccgccgcg
     1321 gaaggagccg tttcctggcg gagctggcgc aggatgccgc cggcgagcaa gatgtcccgc
     1381 tcgcgcggcg agtattcggc gagtagcgtg acctcggtgc cgtcctgcat gcgagccggg
     1441 atctcctcgt ctccattctc cagccgcttc ctgaggtcag gcagctccca cttctgaccc
     1501 tgctcgaatt tgtcgtagtc ggcttcgtct ttgaaacgaa gcggcaatat gccctgggat
     1561 atcaggttcc ggcggtggat gcgggcgaag ctcttggcga tcacggcgcg gacgccgagg
     1621 tgcagcggcg cgagggcggc gtgctcgcgg ctggagccct gcccgtagtt gtgcccgccg
     1681 acgatgaagc caggccccat ctctttagcc ttcttgtggt actcggggtc gaagcggcgg
     1741 aaggtgaacc tggcgatctc cgggacgttc gaccggaacg acatgacctc caccccgtca
     1801 ggagcgaggt caccggtcga gatgtcgtca tccaccacta tggtgaccgt agcctcgagc
     1861 gagtccggaa gctcgggggc ctcgggcgga ggcttgatgt tcgggccgcg cgggatctcg
     1921 atgcccgaag cctcatcctc cggcgcgggc tcgaggatct gccggtcatc caccgccggg
     1981 ttagctgggg cctcgggcag ctcagggtac tctccgagct cgcgtgggtc ggtgatgacg
     2041 ccgcgcagag ccgtcgccgc cgccgtcgtc gggctcgtga ggtagacctg gtccgtcagt
     2101 gtcccggaac gtcccgggaa gttcctgtta aaagtcctca cgctcacggc ctccgagggc
     2161 ggcgcctggc ccatcccgac gcacggaccg cacgccggct cgagcatccg cgccccggcc
     2221 cgcacgaggt cggagtacac gccggcctgg gcgatggagt ccaggatctg gcggctcccc
     2281 ggcgtcacgg tcatgacaag ccccggatac accgtctcgt cgtgcaagac cgcagccgcg
     2341 accgcgagat cctggaagcc ggagttgaca gagctcccga tgcagacctg cgccgccttc
     2401 gtgcccgcga cctcgctcac cggcaccacg ttgccaggcg agtgcggctt ggcgatgagc
     2461 ggctccagcc tgtccagctc gatgtgctcg tgctcgtcgt attctgcgcc ctcatcggcg
     2521 gagagctcga cgaagtcgtc gggtcgctgc tgcatctcga gccactcgcg ggtacgctcg
     2581 tcggagggga agatccctgc tgtcgcgccg agctcggcga tcatgttgca gatcgtagta
     2641 cgtcccgtca cgtctatgtt ggcgactcca ggcccgtaga actcgaagac gcgtccgagg
     2701 ccgccacgca ccccgcgacg ccgcaggagc tctaggatca cgtccttcgg cgtcacccag
     2761 tcctgtagct cccctgttag ttcgacgcca acgaccttgg gggctggagt ctcgaagggc
     2821 tggccggcca tgacgagcgc cacgtcgagc ccgccggccc cgatggcgat ggagcttaga
     2881 gcccccgacg aggtggtgtg ggagtcggct ccgatcaggg tcgcccccgg cctggcgaag
     2941 cgctcgacgt gcaggtagtg gcagatgccg ttgcccggcc gcgagtagta catgccgtac
     3001 ttcgctgcga atgcctgcaa gaagcggtgg tcgtcggggt tcttgtggtc gagctggatg
     3061 atgttgtggt ccacgtactg gacggcgaag tctacctgta ccctctccac ccccatccgc
     3121 tcgaactgca ggcaggccat cgtgcctgtc gcgtcctgca acagcgtctg gtctatcctg
     3181 agccctacct cctcgccagg ctctagcgaa ccactcacca tgtggtcctt gaggatcctg
     3241 gtgaccagat tctccgccat agcgcacctc tccctgaaga ctcgtactct cccattctac
     3301 acagcgggta gtggcggttc gcgagccgcc actacccgct cacgattcgc ctgtagacta
     3361 cgcggtactt ctggatgccc tggcgtagct cctccgtggt ctcctcctcg gagctctctt
     3421 tcgagaaccg gcccacgacg tcggcgcgga tgcgccgggc ctccctgtag tcgtcggcga
     3481 cgtcggggtg catgacggcg aggctgcgct cggtctcctc gtcgctctgc gatggatcgg
     3541 agacgaagtt gcgctcctca aggacctcca tgacggtccg gtcggcaagc tctatggacc
     3601 tgctcgggtt gtccacgaag acccgctcga cctcgtccca ttgctcctcg tagcgccccc
     3661 ggctctcctc ggaaagcggc tccacctgcc gctctacgcg cccgcggcgc tcccttagct
     3721 ccctctctgc ttcctcctct gagccccgag cctcggctgt tcgctcgtac tcgctcccga
     3781 actcctccct cgtcctctca cgctgccgct cgctccgctg cacacgcctc cggcgcattg
     3841 aaaagtagac agcgagcagc aggagcacga tcaccgccac cacgacgatg atgatcaagg
     3901 ttgtactact catgcctcct cctcgcgcag gattgtctaa gccgcatcat tacccaaaag
     3961 gcgtcattcg taacgccgcc cggcgcgcgg gtagtgccgg gccgagacag ggagaggcgc
     4021 gcaacggtgc cggcatgaga acgccggcgg cagtgtaagt caaggcttcg gcatcgaggg
     4081 agagacgaga cggtgaccgc gcagggtcgg agcgacccgg acacggcgcc tcctcgccct
     4141 acgttgggcg agtaaagatc agcgcgatag ggaag
//
LOCUS       DASTUV010000234         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_689320, whole genome shotgun sequence.
ACCESSION   DASTUV010000234 DASTUV010000000
VERSION     DASTUV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689320"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="gcvPB"
                     /locus_tag="VFT03_04730"
     CDS             complement(<1..937)
                     /gene="gcvPB"
                     /locus_tag="VFT03_04730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEU4847481.1"
                     /translation="MLGNLIRDKGRDGRRGYTLPKLDVEETSLDEVLPKKARRKEKPR
                     LPETDEPTVIRHYTNLSRQNWGIDTNFYPLGSCTMKHNPRANEAAAAVPGFAGLHPLQ
                     TEDQAQGALRVMHEVQGFLAKISGLPAVSLQPMAGAQGELASILMIRKYFEDAGDHGR
                     TAVIVPETAHGTNPATATMANFKAVEVGLDEHGCVDVDELREMVDETTAALMITNPNT
                     CGVYERNIKEISEVLHEAGAFLYMDGANMNANMGRMRASDAGVDIMHFNLHKTYSTPH
                     GGGGPGSGAIASSEDLAPFRPDPVVEKQDDKYTLVR"
     gene            complement(927..>2036)
                     /gene="gcvPA"
                     /locus_tag="VFT03_04735"
     CDS             complement(927..>2036)
                     /gene="gcvPA"
                     /locus_tag="VFT03_04735"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEU4847482.1"
                     /translation="NVAELPIFVGAGAYDRIVPSAISSITQRGEFLTSYTPYQPEISQ
                     GHLQVVFEFQSVISELTGLEISNASVYDGSNAVAEAALMTARLTKRDPRVAVSEGLNP
                     RYRQVIETYGVEVVELPFEEGTTNYSDVPDDVSGVFVQSPNFYGIVEDIEAASNAAHE
                     VDALAVAVCDPISLAVLESPGALGADVAVGEAQPLGMPLLFGGPYAGYMATHEKFVRQ
                     LPGRIAGETVDQDGRLAYVLTLRGREQDIRRAKANSNICTNQALTALAATVYAALMGP
                     EGMREVAELSVSKAHYLADRLQASGFRLRYPDAPFLWEFAVEMPDVARANEALLEAGI
                     VGGLDLGDGAMLVAVTEKRTRQELDAFVEVVSNAG"
BASE COUNT          325 a          661 c          663 g          387 t
ORIGIN      
        1 gccggacgag ggtgtattta tcgtcctgct tctcgactac gggatctggc ctgaaaggcg
       61 ccaggtcttc ggaggaggcg atggcgcccg agccgggacc tccgccgccg tggggagtcg
      121 agtaggtctt gtggaggttg aagtgcatga tgtccacgcc ggcgtcggag gcgcgcatcc
      181 ggcccatgtt ggcgttcatg ttggccccgt ccatgtagag gaaggcgccg gcctcgtgca
      241 ggacctccga tatctccttt atgttccgct cgtagacgcc gcaagtgttg gggttggtga
      301 tcatgagcgc agccgtcgtc tcgtccacca tctcgcgcaa ctcatcgacg tccacgcagc
      361 catgctcgtc gagcccgacc tcgacggctt tgaagttggc catagtggcc gtcgcagggt
      421 tggtgccgtg ggcggtctcc gggacgatga ccgccgtcct cccgtggtcc ccggcgtcct
      481 cgaagtactt ccggatcatg agtatgctcg cgagctcgcc ctgcgcgccg gccatcggtt
      541 gcaaggagac cgcggggagg ccggagatct tcgccaggaa gccctggacc tcgtgcatga
      601 cccgcagggc tccctgcgcc tgatcttcgg tctgcaacgg gtggagcccg gcgaagcccg
      661 ggaccgctgc cgccgcctcg ttggcacgcg ggttgtgctt catcgtgcag gagccgaggg
      721 ggtagaagtt cgtgtctatc ccccagttct ggcgcgagag attggtgtag tgacggatca
      781 cggtcggctc gtcagtctcc ggaagcctcg gcttctcctt gcgccgcgcc ttcttcggaa
      841 gcacctcgtc gaggctcgtc tcctcaacgt cgagcttcgg gagggtgtag ccacgcctgc
      901 cgtcgcggcc tttgtcccgg atcaggttac ccagcattcg aaaccacctc cacgaaggcg
      961 tcaagctctt gtctggtccg cttctccgtc accgcgacga gcatcgcccc gtcgcccagg
     1021 tccagccctc cgacgatccc ggcctcgagc aaagcctcgt tggctctcgc cacgtcgggc
     1081 atctcgaccg cgaactccca caagaacggc gcgtcagggt accgtaacct gaagcccgaa
     1141 gcctgaagcc tatctgccag ataatgagcc ttcgagacgg agagctcggc gacctcgcgc
     1201 atcccctcgg ggcccatgag ggcggcgtag acggtcgcgg ctaacgccgt gagcgcctgg
     1261 ttcgtgcaga tgttcgagtt ggccttcgcg cgccggatgt cctgctcgcg gccccgcagg
     1321 gtgaggacgt aggcgagcct gccgtcctgg tcaaccgtct ccccggcgat gcgcccgggg
     1381 agttgcctca cgaacttctc gtgcgtcgcc atgtatccgg cgtaagggcc gccgaagagg
     1441 agcggcatcc cgagcggctg ggcctccccc acagcgacgt ccgcgccaag agctccgggg
     1501 ctctccagca cggcgaggga gataggatcg cacaccgcaa ccgccagagc gtccacctca
     1561 tgagccgcat tcgatgccgc ctctatgtcc tcaactattc catagaaatt cgggctctgg
     1621 acgaagacgc cggagacgtc gtcggggacg tcggagtagt ttgtggtccc ctcctcgaac
     1681 ggtagctcca cgacctcgac cccgtaggtc tcgatgactt gccggtagcg tggattcagt
     1741 ccctcggata cggcgaccct gggatcccgc ttcgtcaggc gggccgtcat gagagcggcc
     1801 tcggcgacgg cgttggaacc gtcgtagacc gaagcgttgg agatctcgag tcccgtgagc
     1861 tcggagatca cggactggaa ctcgaagacg acctgcaggt gcccctggct gatctccggc
     1921 tggtacggag tatacgaggt gaggaactcc ccgcgttgcg ttatggagct gatggcggaa
     1981 ggcacgatcc tgtcgtaggc tcccgcgccg acgaatatcg ggagttcggc tacgtt
//
LOCUS       DASTUV010000235         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1570757, whole genome shotgun sequence.
ACCESSION   DASTUV010000235 DASTUV010000000
VERSION     DASTUV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570757"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..587
                     /locus_tag="VFT03_04740"
     CDS             90..587
                     /locus_tag="VFT03_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4847483.1"
                     /translation="MIIRKDQPPADRGTDERTERLQYSDAGGLTQFGAYVQTLQPGPR
                     SSDRHWHEEEDEFLYMLSGEATVIEEDGTHLLHPGDAACWPAGTANAHQVVNRSNAPC
                     TYLIFGTRMAYDTVHYPDVGKILYDEAGTWRLHRTDDGSLIRGGTIEQLRPLTRWDPK
                     TNQAP"
     gene            886..2394
                     /locus_tag="VFT03_04745"
     CDS             886..2394
                     /locus_tag="VFT03_04745"
                     /inference="COORDINATES: protein motif:HMM:NF011663.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HEU4847484.1"
                     /translation="MAQASAASGDGGSGGTSLRVKVQRFGAFLSGMVMPNIGAFIAWG
                     LITAIFIPDGWYPNERLAELVEPMIFYLLPLLIAYTGGRIVYGARGGVVGAIATIGAI
                     VGSDVPMFLGALIMGPLGGYLIMKFDDLVAHRVRQGFEMLVSTFSAGIIGGTLAIIGV
                     LAAGPIITSISNTLGAAVDFIVALGLLPLVSIIVEPAKVLFLNNAINHGILAPLAIDQ
                     AQEAGKSILFMVETNPGPGVGILLAYSIFGTGLAKSSAPGAAVIQFFGGIHEIYFPYV
                     LMKPALILAAIAGGASGIFTATVFNAGLVSTPAPGSIFAYIAMTPRGGFVGVMSAVFV
                     AAVVSFLIASVIIRASADTDEDDEQSLGMAAAQMEAMKGSKSSVAGTLTGDGKTEDGK
                     TEGEATPKFDQVKSIVFACDAGMGSSAMGASLLRNKVKEAGIEDVSVTNASINNLSED
                     TDLVITHKDLTDRARAKVPNAIHVSVDNFLGSPKYDEVVTELKESREAQLQE"
     gene            2488..>3314
                     /locus_tag="VFT03_04750"
     CDS             2488..>3314
                     /locus_tag="VFT03_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU4847485.1"
                     /translation="MIEVLLRHPEGLTAGDIASRLGVSARTVHRDLQPASEFLGSRGL
                     TLEKQSGRGLRVEGSAEAREQTLEALREAGTSVLTPEGRRLSLLRTLLGADEPIKLRA
                     LASGLKVSVGTVSRDLDEVETWLADFRLSLLRKRGYGVEVLGTEADRRLAMSRLIQEN
                     LDEAALLPLAEAPGDIPDGLVADHVSDRLMGMIDEERLRVVEALTGEEVGRLPYAIAD
                     SAFVGLSVHVALMVERLLQGGKVEVDDDILQRLRKTAEYEHARSLARAIEDAFQIDMP
                     "
BASE COUNT          642 a         1012 c         1080 g          580 t
ORIGIN      
        1 tagaacctgc tcaccttgca cggtacttcg agggaggcag agccaggttc acccaaaggt
       61 agtacggggc acacctatgg aggaccgaga tgatcattcg gaaagatcag ccaccggcag
      121 atcgggggac cgatgaacgg acggagcggc tgcaatattc cgacgcgggc gggttgaccc
      181 agttcggcgc ctacgtccag acgctgcaac cggggccgcg ctcttccgac cggcactggc
      241 acgaagagga agacgagttc ctctacatgc tttctggcga agcgaccgtg atcgaggagg
      301 atggcactca cctgcttcat cccggcgacg cggcctgctg gccggcgggc acggcgaacg
      361 cccatcaggt cgtgaaccgt tcgaatgcac cctgcaccta cctcatcttc ggcacccgga
      421 tggcgtacga caccgtccat tacccagacg tagggaagat cctgtacgac gaggccggca
      481 cctggcggct gcaccgtacc gacgacggat ccctcataag gggaggaaca atcgaacaac
      541 tccgtcccct tacgcggtgg gaccccaaaa ccaaccaggc gccatagcac cgactgccct
      601 gcgcccggta cctcggcgcc ggcgcactcg agcgccggcg gacgtctcgt ctccccctct
      661 tctcgacacc gctttgacct ctgtgtggac taccaggttc gtgggtggtg gtcgatcact
      721 ctctccatac cccgccagat gtcgtaccgt cctcactatt ccacttttgt caacaactcg
      781 tgtgtccgga tggtactaag tacatattgc acaagcgcgt cgacggttct aggattaggg
      841 tgggagagta ggctaaatct cggtgaacgg aaagggagaa gaataatggc acaagcaagt
      901 gctgcgagcg gtgacggcgg gtcgggaggc accagcctca gggtgaaggt ccagaggttc
      961 ggggccttcc ttagcgggat ggtcatgccg aacatcggcg cgttcatcgc gtggggtttg
     1021 atcacggcga tattcatacc cgatgggtgg tacccgaacg agcggctggc cgagctggtg
     1081 gagcccatga tcttctacct gctgccgctc ttgatcgcct acacaggtgg ccggatagtc
     1141 tacggtgcgc gaggcggcgt ggtcggcgcg atagccacca tcggcgccat agtcgggtcc
     1201 gacgtaccga tgttcctcgg cgcgctcatc atggggcccc tcggtggata cctgatcatg
     1261 aagttcgacg acctcgtcgc acacagggta cgccagggct tcgagatgct ggtgagcacc
     1321 ttttccgccg gtatcattgg cggcacactc gccatcatcg gtgtcctcgc cgccggtcct
     1381 atcatcacct ccataagcaa cacactcggg gccgccgtgg acttcatcgt ggccctcggg
     1441 ctcctgccgc tcgtgagcat catcgtcgag ccggcgaagg tgctcttcct gaacaatgcg
     1501 ataaaccacg gcatcctggc cccgctcgcc atagatcagg cccaggaggc cggcaagtcg
     1561 attcttttca tggtcgagac gaatcctggt ccgggggtcg gcatcctgct ggcgtacagc
     1621 atcttcggaa ccggcctcgc caagtcctcg gcaccgggag ccgcggtcat ccagttcttc
     1681 ggcggaatac acgagattta cttcccctac gtcctgatga agccggccct gatcctcgcg
     1741 gccatcgccg gcggggcgag cggcatcttt acggccaccg tcttcaacgc tggcctcgtc
     1801 tcgacgccag ctccgggtag catcttcgcc tacatagcca tgacgccgcg cggcggcttt
     1861 gtcggcgtga tgtcggccgt attcgtagcg gccgtcgtat cgttcctcat agcctccgtg
     1921 ataatccgcg cttctgccga cactgacgag gatgacgagc agagcctggg catggctgcc
     1981 gcgcagatgg aagcgatgaa gggcagcaag agcagcgtgg ccggcacgct gaccggggac
     2041 ggaaagaccg aggacggaaa gaccgaaggc gaggctaccc ccaagttcga ccaggtgaaa
     2101 tccatcgtgt tcgcctgcga cgcaggaatg ggctccagcg cgatgggagc gtcgctgctc
     2161 aggaacaagg tcaaggaggc cggcatagag gacgtctccg tgaccaacgc ttccatcaac
     2221 aacctctccg aggatacgga cctcgtcatc acccacaagg acctcaccga ccgtgccagg
     2281 gcgaaagtgc cgaacgcgat tcacgtctcg gtggacaact tcctcggcag ccccaagtac
     2341 gacgaggtgg tgaccgaact gaaggaaagc cgggaagcgc agctccagga atagacaaga
     2401 agagagacaa gaagaggtga tcgcgggaac agcggccacg cgggttcagg gcctggcgtg
     2461 atggaactct cggcgcgaga gatacaggtg atcgaggttc tgttgcgcca cccggaagga
     2521 ctcaccgccg gcgacatcgc cagccggctc ggcgtgagcg cgaggaccgt tcatcgcgat
     2581 ctgcagccag cctcggagtt cctcggttcg cgcggcctca cgctcgagaa gcagtccggg
     2641 aggggcttga gggtcgaggg gtcggccgag gcgcgggagc agacgctgga agccttgcgc
     2701 gaggcgggta cttcggtgct cacgccggaa gggaggcggc tctctctgct gcgcacgctc
     2761 ctcggggcgg acgaaccgat caagctgcga gcgctggcga gcgggttgaa agtctcggtc
     2821 gggacggtca gccgggacct ggacgaggtg gagacctggc tggcggactt ccgtctctcg
     2881 ctcttacgta aacgcggtta cggggtcgag gttctcggga cggaggccga tcgtcgcctg
     2941 gcgatgagcc gcctgatcca ggagaacctc gacgaggcgg cgctccttcc ccttgcggag
     3001 gcgcccggag atatacccga tggtctggta gcggaccatg tctccgaccg gttgatgggg
     3061 atgatcgacg aggaacggct gcgggtggtc gaggccctga ctggagagga ggtggggcgc
     3121 ctgccgtacg ctatcgccga cagcgccttc gtgggcctga gcgtccacgt cgcgctgatg
     3181 gtcgagcgtc tcttgcaggg cggaaaggtc gaggtggatg acgatatcct gcaaaggctc
     3241 cggaagacgg ccgagtacga gcacgctcgg agtctggccc gggccatcga agatgccttt
     3301 caaatagaca tgcc
//
LOCUS       DASTUV010000236         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_590330, whole genome shotgun sequence.
ACCESSION   DASTUV010000236 DASTUV010000000
VERSION     DASTUV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590330"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFT03_04755"
     CDS             <1..1117
                     /locus_tag="VFT03_04755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEU4847486.1"
                     /translation="GLASVALLSALMIMACAHPHSQLGLRLLGGQPLRWIGQRSYGIY
                     LWHWPVFMITRPQLDVPLEGFPVLVIRVGLTVVLAELSYRFVETPIRRGALGRAWREL
                     RQAQGYRRRQLSLLWAGAAMSVVAICVALGVAVSQAQQPERPSYLPSVNAIHTKSPPE
                     TSGTAERAAADKSEGEQAVADTSEGEHQMVTRRIAAVRKAEENPPREKRAGAEAPVGP
                     VSAVGDSVMLGAAGQLEKDIPNLTVIDAEVGMQAYTAVDVLNARRAAGELGQVVVVHI
                     GNNGTYTEEEFDQTMQALEGVRRVVFVNVNVPRVWEEPNNEVISEGVGRYPNAVLADW
                     YSASVDHPEYFVEDGVHLQIKGQKVYAALIAEKIKAP"
     gene            1270..2232
                     /locus_tag="VFT03_04760"
     CDS             1270..2232
                     /locus_tag="VFT03_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4847487.1"
                     /translation="MIEFRDVSKTYPGSDTSVVNHLSFEVPEGEICVLVGPSGCGKTT
                     TMRMVNRLIEPTDGEILIEGEPNTAVSGTQLRRKIGYAIQQIGLFPHRTIATNIATVP
                     SLLGWDRKRIGERVDELLELVGLAPDDYRDRYPAELSGGQQQRVGVARAMAADPPIML
                     MDEPFGAVDPITRERLQDEFLNIQQNIKKTIVFVTHDIDEAIKMGDKIAILKQGGFLA
                     QYDTPENILSNPNSEFVASFVGNDRILKRLSLSKVGDMDLEPANGGAGDLPRISGNLT
                     VKDALSELIGSGHSEAVVEDDGKKRLLTIDAIEELSRAGGRSRG"
     gene            2225..2941
                     /locus_tag="VFT03_04765"
     CDS             2225..2941
                     /locus_tag="VFT03_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847488.1"
                     /translation="MADPGAFGLALLQDLSSIEEPKLIEWDWIRENFWEDIVPAIQGH
                     IFLSFVSVAIALAISLPTGILVSRYRKAYPPVTFITGLLYSIPSLALFGILISIPGIT
                     IGPTPVIIALVAYSLLILIRNIVAGLDAVPAETIDAARGMGLTQMQVLFKVELPLALP
                     VIVAGIRIATVTIIGIATIGAYIAGGGLGKMIFDGINRSFPTMIITGAVLATALAIVA
                     DLSLLALERFLTPWARRRRA"
     gene            2941..>3582
                     /locus_tag="VFT03_04770"
     CDS             2941..>3582
                     /locus_tag="VFT03_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847489.1"
                     /translation="MDAILDVLERPDFFELLRQHILLSAVALLIGIVIALPVAIAIRN
                     TPLAAALAVNIGNIGRAVPSLAILALCQPFLGFGFDTALVALTALAIPPILINASTGL
                     REVSRDVIDAARGMGLSGGQILTSIQLPIAAPVIFAGIRTSAVQVVASATLATFIGGG
                     GLGELIVEGTQLNDTTILLAGSLSVAVLAIITELGFGVLERGMTPKGLKIAQKR"
BASE COUNT          656 a         1185 c         1109 g          632 t
ORIGIN      
        1 cggcctggcc tccgttgcgc tccttagcgc actgatgatc atggcgtgcg cccaccccca
       61 ctctcagctt ggcttgagac tgctcggagg ccaaccgctg cgctggatcg ggcagcgctc
      121 ctacggcatc tacctctggc actggcctgt attcatgatc acccgccccc agctcgacgt
      181 gcctctagag ggctttcctg tgttggtgat acgagtgggg cttacggtgg tgcttgctga
      241 gctctcctac cgctttgtgg agacccctat caggaggggt gctcttgggc gagcctggcg
      301 cgagttgcgc caggcgcaag ggtacaggcg caggcaactc tctctgctgt gggccggagc
      361 cgccatgtcc gtcgtggcga tctgcgtagc actaggcgtg gccgtctcgc aggcccaaca
      421 gccggagcgg ccgtcctacc tcccgtccgt caacgcgatc cataccaaga gtccgccaga
      481 gacgtccggg accgcagaga gagcagccgc cgacaagtcc gagggtgagc aagcggtcgc
      541 cgacacatcc gagggcgagc accagatggt cacgcggcga atcgccgcgg tccggaaggc
      601 agaggagaac ccacctaggg agaagagggc gggcgcggag gcgccggtcg gtccggtaag
      661 cgcggtcggc gactcggtga tgctcggggc agccgggcag ctcgagaagg acataccgaa
      721 cctcacggtt atcgatgcgg aggtcggtat gcaggcctac acggccgtgg acgtcctcaa
      781 cgcacgccgg gccgccggcg agctcggcca ggtcgtcgtc gtccacatcg gcaacaacgg
      841 cacatacacc gaggaggagt tcgaccagac aatgcaggcg ctcgagggcg tgcgccgggt
      901 cgtgttcgtg aacgtcaatg tcccccgcgt ctgggaggag cccaacaacg aggtcatttc
      961 ggagggcgtg gggcgttacc cgaacgccgt gctcgctgac tggtactcgg cgagcgtaga
     1021 tcacccagag tacttcgtcg aggatggggt gcacctccag ataaagggtc agaaggtcta
     1081 cgcagctctg atcgccgaga agatcaaagc cccctagggc ctagcggaac cgggtgaact
     1141 atcctgtgga gattgccgta tacttctgca aatcgttccc cgtatagctt ggaggagtcg
     1201 tggtcggtct ttcgagatcc gttcttgcct ggcgccagcg gtactatccc tgggcgcaca
     1261 ggagatgcaa tgatcgagtt tagggacgtg agcaagacct atccaggctc cgacacatcc
     1321 gtcgtcaacc atctctcctt cgaggttcca gaaggagaga tttgcgttct ggttggtcct
     1381 tcggggtgcg gcaagacaac gaccatgcgg atggttaacc ggctcatcga gcccacggac
     1441 ggcgagatcc tcatagaagg cgagccgaac accgccgtga gcggcaccca gctgcggcgc
     1501 aagatcggct acgccatcca gcagataggc ctcttccccc accgcaccat cgcgacgaac
     1561 atcgccaccg tcccgagcct cctcggttgg gacaggaagc gcataggaga gcgcgttgac
     1621 gagctcctcg agctcgtcgg cctcgcccct gacgactaca gggaccgcta tcctgcggag
     1681 ctctcgggcg gtcagcaaca gagggtcggt gtagcgcggg cgatggcggc ggaccccccg
     1741 atcatgctca tggacgagcc cttcggcgct gtcgacccta tcacgcgcga gcggctccag
     1801 gacgagttcc tcaacatcca gcagaacata aagaagacca tcgtattcgt cacgcacgac
     1861 atagacgagg ccataaagat gggcgacaag atcgccatcc tcaagcaggg tggcttcctc
     1921 gcccagtacg acaccccgga gaacatcctc tcgaacccga actccgagtt cgtcgcctcc
     1981 ttcgtcggca acgaccgcat cctcaagcga ctctcgctga gcaaagtcgg ggacatggat
     2041 ctggagcccg cgaacggcgg cgccggggac ctcccccgca taagcgggaa cctgaccgtc
     2101 aaggacgcgc tctccgagct gatagggtcc ggacacagcg aggccgtcgt ggaggacgac
     2161 ggcaagaaga ggctgctgac aatagacgcc atagaggagc tctcccgcgc cgggggccgc
     2221 tcgcgtggct gaccccggcg ccttcggcct ggcgctcctc caggacctct cttcgatcga
     2281 agagccgaag ctaatagagt gggattggat ccgcgagaac ttctgggagg acatcgtgcc
     2341 cgcgatacag ggccatatct tcctctcgtt cgtctcggtg gcgatagcgc tcgcgatctc
     2401 gctccccacc ggcatcctgg tctcccgcta tcgcaaggcc tacccgccgg tgacgttcat
     2461 taccgggttg ctctactcca taccgagcct ggccctcttc ggcatcctca tctccatccc
     2521 cggcattacg atagggccca cgcccgtcat catcgcgctg gtggcctact cgctcctcat
     2581 cctgatccgc aacatcgtcg ccgggctcga cgcggtcccg gccgagacca tagacgccgc
     2641 gcgcgggatg ggccttaccc agatgcaggt cctcttcaag gtcgagctac ctctggcgct
     2701 tccggtgatc gtagccggca ttcgcatagc gacggtgacg ataatcggga tcgcgaccat
     2761 aggcgcctac atcgccggcg gcggcctggg gaagatgatc ttcgacggca tcaaccgcag
     2821 cttccccacc atgatcatca ccggcgccgt tctcgcgaca gccctcgcca tcgtcgccga
     2881 cctctcgctc ctcgccctgg agcgcttcct gacgccgtgg gcacgcagga ggagggcgta
     2941 gtggacgcga ttctcgacgt cctcgaacgc ccggacttct tcgagctgct ccggcagcac
     3001 atccttctct cggcggttgc gctactcatc ggcatcgtca tcgcgctgcc cgtagcgatc
     3061 gcgataagga acacccctct ggccgcagcg ctcgccgtca acatcggcaa catcgggcgc
     3121 gccgttccct cactagccat cctggcgctc tgccagccct tcctcggctt cggcttcgac
     3181 acggcgctag tcgcgctcac ggctttagcg atacctccga tcctcatcaa cgcctccacg
     3241 ggcttgaggg aggtgagccg ggacgtcata gacgccgcgc gcgggatggg gctctccggc
     3301 gggcagatcc ttaccagcat acagctcccg atagccgcgc cggtgatctt cgccggcatc
     3361 cgcacctcgg ccgtgcaggt ggtagcgagc gctactcttg cgacgtttat cggaggcggg
     3421 ggccttgggg agctgatcgt agagggcacc cagctcaacg acaccaccat acttctcgcg
     3481 ggctcgctct cggtggcagt cctggcgatc atcacggagc tcggtttcgg cgtgctggag
     3541 agaggcatga cccctaaagg tttgaagatc gcgcagaagc gc
//
LOCUS       DASTUV010000237         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_726484, whole genome shotgun sequence.
ACCESSION   DASTUV010000237 DASTUV010000000
VERSION     DASTUV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726484"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFT03_04775"
     CDS             <1..1152
                     /locus_tag="VFT03_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU4847490.1"
                     /translation="DVKPHNILLDENGRPKISDFGIARALDATYATRTGSYLGTALYS
                     PPEQLKGEKVTPKSDVYSLGATLYHAATGQPPFAGAPIEIASQHVSREPTPPRELNDA
                     VSPALEALILDCMKKDPDARPSADEVRLGLLEAGRGVHATEIYPEPQATGPTRTESAG
                     PTAAPRRGTAQSPPGGARWGRRDRRGGILVALAVVAVLAVIGALAVPGLIGGGKDQAG
                     GEPPQNEAGQNPSGGGQNAQAGGGGQQAASQPASASAGQAGGDQAQASGGSSEAQAAE
                     RTVQEFYTSSSAGNYDRSAQLLSESWRQSTFPNRATFEGTFDKVESVEFIEGPTAERS
                     GDTATVTGRTRATLTDRVELNEGTWSLVKENGEWKIDGWTVNNIDSRPA"
     gene            1204..2337
                     /locus_tag="VFT03_04780"
     CDS             1204..2337
                     /locus_tag="VFT03_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4847491.1"
                     /translation="MAAIVGGLVTALFIFVGITGLDDEPQDVTIREASPPVESASGDV
                     AGAPVREVYSRTGPGVVSVDVASSERGPAGGSGFVLDEQGHIVTNQHVVEGAEDVSVR
                     FARGVREVAEVVGEDPSTDVAVLKVDAPEEILEPLTLGDSDSVGVGEPVIAIGNPLNV
                     GISVTTGIVSGTERPIDAPNGYTIDGAVQTDAAINPGNSGGPLLDSRGTVIGVNAQIA
                     SESGGFEGLGFAVPIDTVKGVVEQLITTGEVVHGYIGVRMFTIGVDELAAYTGLSEEQ
                     LADEYGLPGNGAIVSEVSAGGPAAKAGIEGGEEVEIEGMPEGVPIGDVITEVEGEPVA
                     SPEDVIEAVNAAKPGDKLTLTVVTPGEEARRVGVTVGVQPEGT"
     gene            2334..3047
                     /gene="rpiA"
                     /locus_tag="VFT03_04785"
     CDS             2334..3047
                     /gene="rpiA"
                     /locus_tag="VFT03_04785"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU4847492.1"
                     /translation="MSGVVRAGDSEGLKRDAGYAAVDRYVESGTTVGLGSGTTAAFVV
                     LRIGELLSSGTLRDVRGIPTSERTAALAREVGVPLATLSEARPSLTIDGADEIGPDLT
                     VIKGRGGALLREKIVAAASHRGLVIVADGSKPVDTLGAGPLPVEIEPFGWETTLEALG
                     ALGCEPRLRVDSGDPQHPFVTDGGHYTADCLFPRIPDPASLETRIKRIPGALECGLFV
                     GLAHAAVVARASGVEIIEV"
     gene            complement(3191..3457)
                     /locus_tag="VFT03_04790"
     CDS             complement(3191..3457)
                     /locus_tag="VFT03_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847493.1"
                     /translation="MDRALIQKVVDEARSEEANLVIWDRRDTFTVEQDDLADLEVGDD
                     HLRVKMQEGKATVYVDLDQIYKLVVERERSRRSGSRAGFGAASS"
     gene            3570..>3826
                     /locus_tag="VFT03_04795"
     CDS             3570..>3826
                     /locus_tag="VFT03_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847494.1"
                     /translation="MKNLARKIKDLDRALFRAIFRHKWPPMTTLMRAFTVAGTAGALW
                     GFIAAGLFLITGLRLSHLLVPWVAIAASWTLAEGAKYLFNRA"
BASE COUNT          682 a         1230 c         1344 g          570 t
ORIGIN      
        1 gacgtaaagc cgcacaacat cctcctcgac gagaacggcc gcccgaagat ctcggacttc
       61 ggcatcgccc gcgcgctcga cgcgacgtac gccacccgga cggggtctta cctcggcacc
      121 gcgctctact ccccccctga gcagctgaag ggcgagaagg taacgccaaa gagcgacgtc
      181 tactcgttag gcgcgaccct ctaccacgcg gcgacgggcc agccgccctt cgccggagcc
      241 ccgatagaga tcgccagcca gcacgtctcc agggagccga ctccgccgcg cgagctcaac
      301 gacgcggtga gcccggcgct ggaggccctg atcctagact gcatgaagaa agaccccgac
      361 gcccgcccga gcgccgacga ggtgcggctg ggtctgctgg aggccggccg cggcgtccac
      421 gccaccgaaa tctatcccga gccccaggcg acgggtccca ccagaacaga gtccgccggt
      481 cccaccgcgg caccgcgacg gggcaccgcg caaagtccgc ccggtggcgc aaggtgggga
      541 cgtcgggaca ggcggggagg gatcctggtc gccttggcgg tcgtggccgt gctcgccgtc
      601 atcggcgccc tcgccgtccc cgggctcatc ggcggaggta aagatcaggc tggaggcgaa
      661 cccccgcaga acgaggcggg acagaatccg tccggaggag ggcagaacgc gcaggcagga
      721 ggcggtggcc agcaggccgc ctcgcagcca gcgtcggctt cggcagggca ggcgggagga
      781 gatcaggcac aggcgagcgg cggttccagc gaggcgcaag ccgccgagag gacggtgcag
      841 gagttctata cctcgtcgtc cgcaggtaat tacgacaggt cagcccagct tctcagcgag
      901 agctggcggc agagcacgtt tccgaaccgg gccaccttcg aaggcacgtt cgacaaggtg
      961 gagagcgtag agtttatcga ggggccgacc gccgagcgat ccggtgacac ggcgaccgtc
     1021 accggccgca ccagggccac gctgaccgac cgggtcgagc tgaacgaggg tacctggagc
     1081 ctcgtgaagg agaacggcga gtggaagata gacgggtgga ccgtcaacaa catcgactcc
     1141 cgacccgcgt gataggcgag agctgaggaa gagcgggtcc tgagaaccgt actcacagcg
     1201 ttcgtggcgg cgatcgtcgg tgggctcgtc accgcgcttt ttatcttcgt cgggataaca
     1261 ggcctcgatg acgaaccgca agacgtgacg atcagggagg cttctccgcc ggtggagagc
     1321 gcgtcgggag atgtggccgg cgcgccggtg cgcgaggtgt actcgcggac cgggcccggt
     1381 gtggttagcg tggacgtggc ctcgtccgag cgcgggcccg cagggggctc cgggttcgtc
     1441 ctcgacgagc agggtcacat cgtcaccaac cagcacgtcg tggagggagc cgaagacgtc
     1501 tcggtcaggt tcgcacgcgg cgttagggag gtcgcggaag tggtgggcga ggacccctcg
     1561 accgacgtgg cggtcctgaa ggttgacgcg ccggaggaga tcctcgagcc gctgacgctg
     1621 ggcgattcgg acagcgtcgg ggtcggggag ccggtcatcg ccatcggcaa cccgcttaac
     1681 gtggggataa gcgtgacgac cggcatcgtt agcggcacgg agaggcctat agacgcgccg
     1741 aacggctaca cgatagatgg cgcagtccaa accgacgcgg cgataaaccc cggcaactcc
     1801 ggcggaccgc tgctcgactc gcgcgggacg gtcatcgggg tgaacgccca gatcgcctcg
     1861 gagtcaggcg ggttcgaggg cctcggcttc gccgtcccga tagataccgt gaagggcgtg
     1921 gtcgagcagc ttataaccac aggggaggtg gtgcacggct acatcggggt acgcatgttc
     1981 acgatcggcg tggacgagct cgccgcctac actggcctct cggaagagca actcgccgac
     2041 gagtacggcc tgcccggtaa cggtgcgata gtgagcgagg tgagtgccgg cggccccgcg
     2101 gcgaaagccg gcatcgaggg cggcgaagaa gtggagatag agggtatgcc agaaggggtg
     2161 cccatcgggg acgtgataac cgaggtggag ggagagcctg tcgcctcccc agaagacgtg
     2221 atagaggccg tcaacgcggc aaagccaggc gacaagctca cgctgaccgt cgtgaccccc
     2281 ggtgaggagg cgcgacgggt cggggtgaca gtcggcgtgc agccggaggg cacgtgagcg
     2341 gcgtggtgag ggctggagac tcggaggggc tcaagcgcga tgcaggctac gcggcggtgg
     2401 accgctacgt cgagagcggc acgaccgtgg ggctcgggag cggaaccact gccgccttcg
     2461 tagtgctcag gatcggggag cttcttagca gcgggacgct gcgggacgtg cgcgggatac
     2521 cgacctcgga gcggactgcg gcgctcgccc gggaggtcgg tgtcccgctg gcgacgctct
     2581 cggaggcccg gcccagcctc acgatagacg gcgcagacga gatcgggccc gatctcacgg
     2641 tcatcaaagg caggggaggg gctctcctgc gcgagaagat cgtggccgcc gcctcacata
     2701 ggggcctcgt catcgtagcc gacggctcga agccggtcga tactctaggc gccggcccgc
     2761 tgccagtcga gatagagccc ttcggctggg agacgaccct ggaggcgctc ggcgctcttg
     2821 gctgtgagcc ccggctgcgg gtggactcgg gagacccgca gcaccccttc gtcacggacg
     2881 ggggccacta cacggccgac tgcctcttcc cgcggatccc cgatccagcc tccctggaga
     2941 cccggataaa gcgcatcccg ggcgccctgg agtgcgggct cttcgtgggg ctcgcccacg
     3001 ccgcggtcgt cgcgcgcgcg agcggcgtgg aaattatcga ggtttagtag caattcagca
     3061 cgcctgcttc gcaggcttct cagcacgctc cggctaacgc ctccacttgt cagcacgccg
     3121 cctggcgagg gccttctaaa ggcccgagcg tgctgagaag cggaagccga aggctggagc
     3181 gtgctgaagt tcagctgctc gcggcgccga atccggcgcg tgacccggat ctgcgggagc
     3241 gctccctctc caccacgagc ttgtatatct ggtcgagatc cacgtagacg gtggccttgc
     3301 cctcctgcat ctttacccgc aggtggtcgt ccccgacctc gaggtccgcc aggtcgtcct
     3361 gctcgacggt gaacgtgtcg cggcggtccc agatcacgag gttggcctcc tcgctgcgcg
     3421 cctcgtccac taccttctgg atcagtgctc tgtccacgtt tcgaacttcc cttctaaaac
     3481 atctcctgaa acatccgagc tattctactt tacttaccct tgcccggacg cccgggccga
     3541 agacgggtac aatacccggc gatccgagat tgaagaacct cgcacgcaaa ataaaggacc
     3601 tcgacagggc gcttttcagg gcgatcttcc gccacaagtg gcctcccatg acgaccctga
     3661 tgcgcgcctt cacggtcgcg gggaccgcgg gagcgctctg gggtttcatc gccgccgggc
     3721 ttttcctgat tacgggtctg cgtctctcgc acctgctcgt gccctgggta gccatcgcgg
     3781 cctcctggac gctcgccgag ggcgcgaagt acctcttcaa ccgggc
//
LOCUS       DASTUV010000238         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1580429, whole genome shotgun sequence.
ACCESSION   DASTUV010000238 DASTUV010000000
VERSION     DASTUV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580429"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFT03_04800"
     CDS             complement(<1..225)
                     /locus_tag="VFT03_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847495.1"
                     /translation="MPDSAPIEGVLTEREALNRRPVLLAVAVATATVLPAFLTGGLAV
                     QVRGEFHFGAAALGLAVAAFFVTASVASAVM"
     gene            complement(280..1791)
                     /gene="xylB"
                     /locus_tag="VFT03_04805"
     CDS             complement(280..1791)
                     /gene="xylB"
                     /locus_tag="VFT03_04805"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEU4847496.1"
                     /translation="MNVLLGLDVGTGGARAVAVDESGEVVAEASSEYALHSPHPGWTE
                     QDPEDWWGGAKEALGKVAAEVREAGDEVVGLGLTGQMHGSVFLDASGEVIRRALLWND
                     QRTEAQCREITGAVGEERLIQITGNPALTGFQAPKVLWLRDEEPENYSRVARVLLPKD
                     YIRLRLTGEYATDASDAAGTLLLDARERDWSAEILAALEIPAEWMPAVFEGPERTGEL
                     GRSVAGELGLPPGLPVAAGGGDNAAAAVGVGVIDAGLLSSSIGTSGVLFAPADEFAPD
                     PSGRIHAFCHAVPGEYHLMGVTLSAGGSLRWWRETLGKGYDELVEAASEIEPGAEGLI
                     FLPYLSGERTPHLDPRARGSFLGLTARHGVAHMTRAVMEGVVLSLRDSLEIMRRLDLS
                     VRQIRATGGGARSALWRELQADVYGVPIQRTTADEGPAYGAALLAGVAAGVYSDVEAA
                     CSTVRLRGEVTEPDPERTRIYDQLYEVYRSIYPATREAMHRLAELAADSAEPG"
     gene            complement(1794..>2717)
                     /locus_tag="VFT03_04810"
     CDS             complement(1794..>2717)
                     /locus_tag="VFT03_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU4847497.1"
                     /translation="YPGEVNEENVEEVLRVLREYGMDLPVIAAGLHTDPTYALGAFVN
                     PDDRLRRQGIDTLKHGVDLCAQVGASFIIWPGAEGYNYTFQRPYAQVWRGFVEGIAEV
                     TDHANEQGVKVFLEHKNSEPAMKILMKNIGMTLFTIQKVNALGVDTSNLLVNMDWQHL
                     IMNGESLAEYADLLAMESKLGHQHGNDGWGTFDDDNVVGTNFFMQTLELAQTLQDTGY
                     GESGELVGFDLYPYTEDQVAAVRRAILQWEFIWDLARKIDRDTLREAKSRADALAGQR
                     AVYRALGLDEDFEAEVVRRRRESRAARSEEG"
BASE COUNT          421 a          947 c          873 g          476 t
ORIGIN      
        1 catgaccgcc gaggccaccg acgccgtgac gaagaacgcc gcgaccgcga ggccgagagc
       61 cgccgcgccg aagtggaact cgccccgcac ctgcaccgcg agcccaccgg tcaggaaggc
      121 agggagcacc gtggcggtag cgaccgccac agcgagcaga acgggacggc gattcaaggc
      181 ttcgcgctct gtcaggacgc cttctatggg cgcggagtca ggcacgtggc agataatagc
      241 acgcgctctt tgcgctcccc gcagctggag gtgatcgcgt taacccggct ccgccgaatc
      301 ggcggcgagc tcggccaggc ggtgcatcgc ctccctcgtc gcggggtaga tcgagcggta
      361 gacttcgtag agctggtcgt agatcctggt tcgttcggga tccggctcgg taacctcgcc
      421 acgcagccgc accgtcgagc aggccgcttc aacgtcgctg tagacccctg cggccacccc
      481 tgcgagtagc gccgcgccgt aggccggccc ttcgtctgcg gtcgtgcgtt gaatggggac
      541 gccgtacacg tcggcctgca actccctcca gagcgcgctc ctcgccccgc cgcccgtggc
      601 gcgtatctgc cgcacggaca ggtcgagccg gcgcatgatc tccagcgagt ccctcaaaga
      661 caagaccacg ccctccatga cggcacgggt catgtgcgcg acgccatgcc tggcggtgag
      721 cccgaggaag gagcctcgcg cccgcgggtc gaggtgaggc gtccgttccc cgctcaggta
      781 cggcaagaag atgagtccct ccgcgccggg ttcgatctcc gaggccgcct ccacgagctc
      841 gtcgtagcct ttgccgaggg tttcacgcca ccaccgcagg gagccgcccg cggagagagt
      901 aacgcccatc aggtggtact cgccggggac ggcgtggcag aaggcgtgaa tgcgccccga
      961 agggtcgggc gcgaactcat cggcaggggc aaagagtact ccgctcgtcc ctatcgagga
     1021 gctcaggagg ccggcgtcga tgacgcccac ccccaccgcg gcggccgcgt tgtccccacc
     1081 tcctgctgca acggggagac cgggcggcag gccgagctcg cctgcgacgc tccggccgag
     1141 ctcgccggtc ctctccggtc cctcgaacac ggcgggcatc cattcggcgg ggatctcgag
     1201 cgccgccagg atctccgccg accagtccct ctctctggcg tccagcagca gcgtgccggc
     1261 ggcgtcggag gcgtcagtgg cgtactcgcc ggtgagccgg agcctgatgt agtccttggg
     1321 gagcaacact cgggcgaccc tcgagtagtt ctccggctcc tcatccctta gccacagtac
     1381 tttaggcgcc tggaagcctg tgagggcggg attcccggtt atctgtatca ggcgttcttc
     1441 tcctacggca ccggtgatct cgcggcactg cgcctccgtg cgctggtcgt tccacaggag
     1501 cgcgcgccgg atcacctctc ccgaagcgtc gaggaacacg gatccgtgca tctggcccgt
     1561 gagtcccaac ccaacgactt cgtcgcctgc ctcacgtacc tcggcggcga ccttcccgag
     1621 cgcctccttc gcgccccccc accagtcctc agggtcctgc tcggtccagc cggggtgcgg
     1681 gctgtgaaga gcgtattccg aggaagcctc cgctaccacc tctcccgact cgtcgacggc
     1741 gacggccctg gcgccgccgg tgccgacgtc aaggccgagg agtacgttca cggctatcct
     1801 tcctcgctgc gcgccgcgcg cgactcccgg cgtcttctca ccacctcagc ctcgaagtcc
     1861 tcgtccagcc ccagagctct gtagactgcc ctctggccgg cgagggcgtc ggcccggctc
     1921 ttcgcctcac gcaaagtgtc gcggtctatc ttgcgggcga gatcccagat aaactcccac
     1981 tgcagaatgg cgcgcctgac ggcggcgacc tggtcctcgg tgtagggata gaggtcgaag
     2041 cctactagct cgccgctctc cccgtagcca gtgtcctgga gggtctgggc aagctcgagg
     2101 gtctgcatga agaagttggt gccgacgacg ttgtcgtcgt cgaaggtgcc ccagccgtcg
     2161 ttgccgtgct ggtggccgag cttgctctcc atggcgagga ggtcggcgta ctcggctaga
     2221 gattcgccgt tcatgatcag gtgctgccag tccatgttga cgagaaggtt ggaggtgtca
     2281 acgccaagag cgttcacctt ctggatggtg aaaagcgtca tcccgatgtt cttcatcagg
     2341 atcttcatcg cgggctcgct gttcttgtgc tcgaggaaga ccttgacgcc ctgctcgttg
     2401 gcgtggtctg tcacctcggc tatcccctcg acgaagccgc gccacacctg cgcgtatggc
     2461 cgctggaagg tgtagttgta cccctccgcc cccggccaga tgatgaagct tgcccctacc
     2521 tgagcgcaca ggtccacgcc atgcttgagg gtgtcgatgc cctgtctgcg cagccgatcg
     2581 tccgggttga cgaaggcacc caaggcgtat gtcgggtcgg tgtggagtcc ggcggctatc
     2641 acgggcaggt ccatcccgta ctctctcagg acccggagga cctcctcgac gttctcctcg
     2701 ttcacctcgc ccgggta
//
LOCUS       DASTUV010000239         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1349376, whole genome shotgun sequence.
ACCESSION   DASTUV010000239 DASTUV010000000
VERSION     DASTUV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349376"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFT03_04815"
     CDS             complement(<1..610)
                     /locus_tag="VFT03_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU4847498.1"
                     /translation="MMRAPRSVLAVPASNLGMAEKALASQADAVFLDLEDAVAPDEKA
                     EARGKIVQALQELDWGDRSTLYRSNALDTPYFYRDVIEVVEEAGDRLDSILIPKVQRP
                     EDLHVAATLLYQTELSGGLEPGKIKIEGQIESARGLVRVDGIASATGRLMALHFGPGD
                     FAASVRMPQTSIGVIDEWDEVYPGHRFHYAMQRIVVAARAADL"
     gene            complement(607..2112)
                     /locus_tag="VFT03_04820"
     CDS             complement(607..2112)
                     /locus_tag="VFT03_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU4847499.1"
                     /translation="MTERDGEGRYEATGATERRDGEGTHREEAAAEAIGAGSPEDVGQ
                     TTFAATTDSLQQTVGRDRPESGSYHFPAFDDHHPPDKKLIDECVHCGFCLPTCPTYVL
                     FGEEMDSPRGRIYLMNKGLDEEAMDDTMVRHWDLCLGCMACVTACPSGVQYDKLIEAT
                     RAQIERRYERRSDDRAFREMIFQLFPYRNRLRLAAGPLRLYQRFGIGERLRKAGVMDR
                     LPARMRAMEALMPKLGREETIPEFTGPAGEKRRRVGVLLGCVQQVFFSRVNSATVRVL
                     AAEGCEVVAPKSQGCCGALSTHAGREEESLDFARKTIDTFERLDLDNVIVNAAGCGST
                     MKEYGHLLRDDPEYSDRARAFSEKVMDVMEFLAEIGPAAERQPLPLSVAYHDACHLGH
                     AQGIRKQPRTVLKGIPGVEIREIKEAEICCGSAGIYNMVEPEPAAELGERKANNVLKT
                     GAQMLVTSNPGCILQIQASLKKTGNDIPTAHPMEVLDASIRGEPVETLLAR"
     gene            complement(2112..>3036)
                     /locus_tag="VFT03_04825"
     CDS             complement(2112..>3036)
                     /locus_tag="VFT03_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4847500.1"
                     /translation="LSGSDQMLAIDPPERGATIGGIIAANASGPRRYRYGTIRDLIIG
                     ITVVLHDGTVAKAGSKVVKNVAGYDLSKLFTGSLGTLGIIVTANFRLHPIPQVSRTVA
                     VEVESPEAAAGAAQAMMHSQVEPTAVELHYGEDARLLTVLLESIPAGVEEKVEAATFL
                     LNPFGEVRTLSDEETGHLDPSTAAEDETVIKLSAPPADLADVLGTVLGAAKRRGLDQP
                     RITGHAASGVTFVGFSGGDEDARAGFVEELREIWLRRGGSVTLQKAPLSLKRRVGAWD
                     QGGDYLGLIRRVKEKFDPRGGMNPGRFVGGV"
BASE COUNT          452 a         1015 c         1000 g          569 t
ORIGIN      
        1 gaaggtccgc agcgcgggcg gcgacgacta tgcgctgcat ggcgtagtgg aagcggtgac
       61 ccggatatac ctcgtcccac tcatctatca cgcctatgct cgtctggggc atccttacgc
      121 tcgccgcgaa gtcgccgggc ccgaagtgga gggccatgag gcgcccggtg gcgctggcaa
      181 ttccgtccac cctgacgagc cctctggcgc tctctatctg accttcgatc tttatcttgc
      241 ccggttcgag gccgccagaa agctcggtct gatacagcag cgtggcggcg acgtggaggt
      301 cttcggggcg ctggaccttg gggatgagga tgctatccag cctgtccccg gcctcctcga
      361 cgacctcgat cacgtcccgg tagaagtaag gggtgtcgag ggcgttggac cggtagaggg
      421 tcgatctatc cccccagtcg agctcctgaa gagcctggac aatcttcccc cgcgcctcgg
      481 ccttctcgtc gggagctacg gcgtcttcga ggtcgaggaa gacggcgtcg gcctgggagg
      541 cgagggcttt ttccgccatc ccgaggttcg aggcggggac ggccaggaca gagcgcggtg
      601 ctctcatcat ctggcgagca gcgtctccac gggttcgccg cggatggagg cgtcgaggac
      661 ctccatcggg tgggccgtag gaatatcgtt accggtcttc ttgaggctgg cctgtatctg
      721 gaggatgcag ccggggttgg aggtgacgag catctgcgcg ccggtcttga ggacgttgtt
      781 cgccttgcgc tcgccgagct cggcggcggg ctcgggctcg accatattgt agataccggc
      841 ggagccgcag cagatctcgg cttcctttat ctccctgatc tcgacccccg gtatcccttt
      901 cagaaccgtg cgcggctgct tgcggatgcc ctgcgcgtgc ccgaggtggc aggcgtcgtg
      961 gtaggccacg gagaggggca gcggttggcg ctcggctgcg ggtccgatct cggccaggaa
     1021 ctccataacg tccatgacct tctcggagaa ggccctggcg cggtcggagt actcgggatc
     1081 gtcgcgcagg aggtgaccat actccttcat cgtcgagccg cagccggcgg cgttgacgat
     1141 cacgttgtcc agatccagtc tctcgaacgt gtctatggtc ttcctcgcga aatccaggga
     1201 ctcctcctcg cgcccggcgt gggtactgag agcgccgcag cagccctggc tcttgggcgc
     1261 gacgacctcg cagccctccg cggcgagcac ccggaccgtc gcggagttta cccgggagaa
     1321 gaagacctgt tgcacgcatc ccaggaggac gccgacccgc ctgcgcttct cgccggccgg
     1381 tccggtgaac tcggggatcg tctcctccct ccccagcttc ggcatgaggg cctccatcgc
     1441 ccgcatgcgc gcagggagcc gatccatgac ccccgctttt cgaagcctct cgccgatacc
     1501 gaagcgctgg tagagccgca ggggacccgc cgccaggcgc agacggttgc ggtaggggaa
     1561 cagctggaag atcatctccc ggaacgctct gtcgtcggag cggcgctcgt agcggcgctc
     1621 gatctgtgcc cgcgtggcct cgatgagctt gtcgtactgc acgccggagg ggcaggcggt
     1681 gacgcaggcc atgcatccga gacacaggtc ccagtgcctg accatcgtgt cgtccatcgc
     1741 ctcctcgtcg aggcccttgt tcatcaggta aatgcgcccc cttggcgagt ccatctcctc
     1801 gccgaagagc acgtaggtcg ggcaggtcgg caggcagaag ccgcagtgga cgcactcgtc
     1861 tatgagcttc ttgtccggcg gatggtggtc gtcgaaagcc gggaaatggt agctgccgct
     1921 ctcgggccgg tcccttccca cggtctgctg gagcgagtcg gtagtggccg cgaaggtggt
     1981 ctgccccacg tcctccgggc tgccggcgcc gatggcctcg gccgccgctt cctcgcggtg
     2041 cgtaccctcg ccgtcgcgcc tttcggtggc gccggtcgcc tcgtagcgac cctcgccgtc
     2101 acgctccgtc actagacacc tcccacaaac ctgccggggt tcatgccgcc ccggggatca
     2161 aacttctcct ttacccgcct tatgaggcca aggtagtcgc caccctggtc ccacgcgccg
     2221 accctgcgct tcagagacag cggggccttc tgtaacgtta cgctgccccc tctgcggagc
     2281 cagatctcgc gcaactcctc gacgaacccg gcccgggcgt cctcgtctcc gcccgagaac
     2341 ccgacgaagg ttacgccgct ggcggcgtgg cccgtgatgc ggggctggtc gagccctctg
     2401 cgcttcgccg ccccgaggac cgttcccagc acgtccgcga ggtcagcagg tggggcggag
     2461 agcttgatga cggtctcgtc ctcagcggcc gttgatgggt cgagatgacc cgtctcctcg
     2521 tcggagaggg tccggacctc tccgaagggg ttgagcaaaa aggtagcggc ttcgaccttc
     2581 tcctcgacgc cggccggtat gctctcgagg aggaccgtga gcagcctggc gtcctcgccg
     2641 tagtgcagct cgacggccgt cggctcgacc tgggagtgca tcatggcctg ggctgccccc
     2701 gccgccgcct cgggactctc gacctcgact gcgaccgtcc tcgacacctg ggggatcgga
     2761 tgcagcctga agttggcggt cacgatgatg cctaaagtgc ccagagaacc tgtgaatagc
     2821 ttggagaggt cgtagccggc cacgttcttg acgaccttgc tgccggcctt cgccacggtt
     2881 ccgtcgtgca gaacgacggt tatgccgatg ataaggtccc tgatggtccc gtacctgtac
     2941 ctcctcggcc ccgaggcgtt ggccgcgatg atgcccccga tcgtagcccc cctctccggc
     3001 ggatctatgg cgagcatctg gtccgagccg gagagc
//
LOCUS       DASTUV010000240         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1077457, whole genome shotgun sequence.
ACCESSION   DASTUV010000240 DASTUV010000000
VERSION     DASTUV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077457"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFT03_04830"
     CDS             <1..748
                     /locus_tag="VFT03_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU4847501.1"
                     /translation="HRMDPRIKLAATFVMLVASFVIDDFLGFAVALALLALIQIVSRI
                     PLGYLLRGSGFFLGFLLFILAFQVLFYPGEVSDYLWRWGILSVSTEGLRAAGILGLRV
                     IFLYYVTTMLMLTTSLVDLTNGLELIFGPLQRLRVPVNELVLVFVIAIKFVPIFIEEV
                     ERLARAQTARGVPFDEGGAIKRARRLGRLLVPIFIGGFARADVLTTAMNTRCYRGGRY
                     RTKFRQMNTTPSDWLILSLVMIWMIAAWLV"
     gene            complement(800..1906)
                     /locus_tag="VFT03_04835"
     CDS             complement(800..1906)
                     /locus_tag="VFT03_04835"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU4847502.1"
                     /translation="MQVFEKLAEYRYEQLVFCHDKATGLRAVIAIHDTTLGPALGGCR
                     MYPYDSEDEAVVDVLRLARGMTYKAAASGLNLGGGKSVIIGDPERHKSEELFRSFGRY
                     LETLGGRYIVAEDVGTSTEDMENIRIETSHVVGVDVTHGGSGDPSPFTALGVVQGMKA
                     CAEEVFGDASLEGRTVAVQGLGHVGWHLCELLHEEGAGLFVSDLREEVVEQAQQELGA
                     KPIEPDEILSIPCDVFAPCALGAVVNDETLHNFRCSIVAGSANNFLLEPRHGEALVKR
                     CILYAPDYVINAGGLINVADELEGYNEKRATKRVMRIYDSMKSIIAIAKRDGVPTHVA
                     ADTLALERIDAISSIERLHTGHPYGTERRRRETL"
     gene            2010..>2755
                     /locus_tag="VFT03_04840"
     CDS             2010..>2755
                     /locus_tag="VFT03_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU4847503.1"
                     /translation="MQGERLGEGTTAGKIEGLERLREAAANPAPKQAVERQRERGKLT
                     ARERIELLLDPDTFVELDRYRVHRSHNFGLEENKPLGDGVITGYGEVRGRKVCVFSQD
                     FTVFGGSLGEVYAQKICKVMDLALSTGCPIIGINDSGGARIQEGVVSLAGYADIFHRN
                     VLASGVIPQISVVMGPCAGGAVYSPAITDFVFMVEETSHMFITGPDVIKSVTGEEVEF
                     EELGGAMSHNTKSGVAHFASEDEDQCLEDCR"
BASE COUNT          444 a          889 c          894 g          528 t
ORIGIN      
        1 ccaccgcatg gacccccgca taaagctcgc cgccacgttc gtgatgctcg tggcctcctt
       61 cgttatagac gacttcctgg gcttcgcggt ggcgctggcg ctgctggcgc tgatccagat
      121 cgtctcccgc atcccactcg gctacctcct gcggggctcc ggctttttcc tcggcttcct
      181 gctcttcatc ctggccttcc aggtcctctt ctatcctggt gaggtctccg attacctgtg
      241 gcgctggggg atactctcgg tctctaccga ggggctcaga gccgccggaa tactcggcct
      301 gcgcgtgatc ttcctctact acgtcacgac catgctgatg ctgacgacct cgctggtaga
      361 tttgacgaac gggctggagc tcatcttcgg accgttgcag cggctcagag ttccggtcaa
      421 cgagctcgtc ctcgtcttcg tcatcgcgat caagttcgtc ccgatcttta tcgaggaggt
      481 cgagcgcctc gcccgcgccc agacggcccg cggcgtcccc ttcgacgagg gcggcgcgat
      541 caagcgcgcc cggcgtctcg ggcgcctgct cgtcccgatc tttatcggcg gcttcgcccg
      601 cgccgacgtc ctgaccaccg ccatgaacac ccgctgctac cgcggcggcc gataccgcac
      661 caagttccgc cagatgaaca ccacaccctc ggactggctg atactcagct tggtcatgat
      721 ctggatgatc gcagcgtggc tggtttagca gtcagccatc agctatcaac caaaaactag
      781 aaaaaccgac cgctgatagc tacaacgtct ccctgcggcg gcgttcggtg ccgtaggggt
      841 ggccggtgtg gaggcgctct atggagctta tggcgtctat gcgctccagg gctagggtgt
      901 cggctgcgac gtgggtgggc acgccgtcgc gtttggcgat ggcgatgatg ctcttcatgg
      961 agtcgtagat gcgcatgacg cgcttggtag cccgcttctc gttgtagcct tcgagctcgt
     1021 cggcgacgtt tatgagccct ccggcgttaa tgacgtagtc gggcgcgtag aggatgcagc
     1081 gcttgacgag cgcctcgccg tggcgcggct cgaggaggaa gttgttcgcg ctgccggcga
     1141 cgatggagca ccggaagtta tgcagggtct cgtcgttgac gacggccccg agggcgcagg
     1201 gggcgaacac gtcgcacggg atggagagga tctcgtcagg ctcgattggc ttcgccccga
     1261 gctcctgctg cgcctgctcg acgacctcct cgcgcaggtc gctcacgaag agacccgccc
     1321 cttcttcgtg gagtagctcg cacaggtgcc agccgacgtg cccgagcccc tgcaccgcca
     1381 ccgtccggcc ctccagggag gcgtcgccga atacctcctc ggcgcaggcc ttcatccctt
     1441 gcacgacgcc cagagcggtg aagggcgagg ggtcgcccga gccgccgtgg gtcacgtcca
     1501 cgccgacgac gtgggaggtc tcgatgcgga tgttctccat atcttcggtc gaggtgccga
     1561 cgtcttcggc gacgatgtat cggcctccca aagtctcgag gtagcggccg aaggagcgga
     1621 agagctcctc ggacttgtgg cgctcggggt cgccgatgat gaccgacttc ccgcccccga
     1681 ggttgaggcc gctcgcggcg gctttgtagg tcatgccgcg cgccagcctt agcacgtcca
     1741 cgacggcctc gtcctccgag tcgtaggggt acatccggca gccgccaagc gcaggcccga
     1801 gcgtggtgtc gtggatcgcg atcacggccc gcagccccgt ggccttgtcg tggcagaaga
     1861 cgagctgctc gtagcggtac tcggcgagct tctcgaagac ctgcacgccg tctcctttcc
     1921 ctcgtccgga ggtccctata ttttactacc ttcttcctcg ttaccagcgg aaaaccggcg
     1981 gttttccgcg tgctagaatg cctgctggta tgcagggtga gaggcttgga gaaggcacga
     2041 ccgcggggaa gatcgagggc ctcgaacgcc tgcgcgaggc ggcggcgaac ccggctccca
     2101 agcaggccgt cgagcgccag cgcgagcgcg gaaagctcac cgcgcgggag cgcatcgagc
     2161 tgctgctcga cccagacacc ttcgtagagc tcgaccgcta cagggtacac cgctcgcaca
     2221 acttcgggct cgaggagaac aagcctctgg gcgacggtgt gatcaccggc tacggggagg
     2281 tgagggggag gaaagtctgt gtcttctccc aggacttcac ggtgttcggc ggctctctgg
     2341 gcgaggtgta cgcgcagaag atctgcaagg tcatggacct ggccctgagt accggatgcc
     2401 cgattatcgg gatcaacgac tccggcgggg cgaggattca ggaaggcgtt gtctcgctgg
     2461 ccggctacgc cgacatcttc cacaggaacg tgctggcttc gggggtcata cctcagatct
     2521 ccgtcgtcat gggaccctgc gccgggggcg cggtctactc ccccgcgata acggacttcg
     2581 tcttcatggt cgaggagacg agccacatgt tcataaccgg ccccgacgtc ataaagagcg
     2641 tcacgggcga ggaggtcgag ttcgaggagc tgggcggcgc gatgtcgcac aacacgaagt
     2701 cgggcgtcgc ccacttcgcc tccgaggacg aggaccagtg cctggaggac tgccg
//
LOCUS       DASTUV010000241         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1315316, whole genome shotgun sequence.
ACCESSION   DASTUV010000241 DASTUV010000000
VERSION     DASTUV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315316"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="VFT03_04845"
     CDS             complement(<1..1889)
                     /locus_tag="VFT03_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU4847504.1"
                     /translation="MANRLANETSPYLLQHKDNPVDWYPWGEEALERARNEDRPILLS
                     VGYSACHWCHVMERESFEDEATAQIMNEHFVNIKVDREERPDIDSIYMSAVQALTRGG
                     GWPMTVFLTPDGAPFYGGTYFPPAPRGGLPSFQQVLLSLADAYENNRDEVMKSAEGVR
                     EYLKAATGAAVPRSVLGTGLLDNAAASLSGELDRRFGGFGGAPKFPQAMNLEVLLRHH
                     HRTGDRDALSGVEVTLRAMANGGIYDQLGGGFARYSVDRYWLVPHFEKMLYDNALLGR
                     LYLEAYQATGDSFYRRIAEETLDYVVRDMTSPEGGFYSAEDADSEGEEGKFYVWTPEE
                     IRGVLDPEDAGLAERFWDVTERGNFEGRNILHVPRPPEVVAAEFGLSPEELWERVEGI
                     RSRLFAAREERARPGRDEKVLASWNGLMLHSFAFAASVLDREDYREVAERNAAFLLEK
                     LRVDGRLKRSYKDGRARFNGYLEDYAMVADGLVTLYEATFEPRWLAAAQDLVDAVMEL
                     FWDEERRTFYDTPADHEELVTRPRDVYDSAAPSGNSVATDVLLRLALLLDREDYRARA
                     EAALEELSGGMEKLPGGFGRLLAALDFYLSRPREVVIVGDPASPDTQALVDAVYASYL
                     PNKVVA"
     gene            2183..2326
                     /locus_tag="VFT03_04850"
     CDS             2183..2326
                     /locus_tag="VFT03_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847505.1"
                     /translation="MCYSYREYRMEAEARRQRQEEERRRREEQAKKAEKERAAREREL
                     VRA"
     gene            2426..3451
                     /locus_tag="VFT03_04855"
     CDS             2426..3451
                     /locus_tag="VFT03_04855"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEU4847506.1"
                     /translation="MNLQELEKTARALVAPGKGILAADESFGTIGKRFEAVGIESSED
                     SRRAYREMLFTTPNIGEYLSGVILFEETLRQKASDGRPLAKVLEDQGIIPGIKVDKST
                     VPMPLSPNEKFTQGLDGLHDRLSEYREMGARFTKWRAVITIGSGIPTKSCVEANAEAL
                     ALYASFAQDHDLVPIVEPEVLIDGDHSIEQSYEATLWTLHETFDEIHEHGVELSALLL
                     KPNMVISGKEASDQANVEEVARYTVECLLRTVPPAVPGIVFLSGGQTDLQATQHLNAM
                     NTIYEGLPWELSFSYARALQGQPMEIWGGKAENVERAQKIFHHRARMNSAARDGRYSE
                     EMEQEAA"
BASE COUNT          682 a         1190 c         1229 g          592 t
ORIGIN      
        1 gcgacgacct tgttgggcag gtagctggcg tagacggcgt ctaccagggc ctgggtgtcg
       61 ggcgaggccg ggtcaccgac aatcacgacc tcgcgcgggc gggagaggta gaaatcgagg
      121 gcggccagga ggcggccgaa accgcccggc agcttctcca tgccgccgct cagctcctcg
      181 agtgcggcct cggcgcgggc gcggtagtct tccctgtcga ggaggagcgc gagcctgagg
      241 aggacgtcgg tggcgacgga gttgcccgag ggcgcggcgc tgtcgtagac gtcgcgcggg
      301 cgtgtcacga gctcctcgtg atccgccggc gtgtcgtaga aggtcctccg ctcctcgtcc
      361 cagaacagct ccatgacggc gtcgacgagg tcctgggccg ccgcgagcca ccgcggctcg
      421 aacgtcgcct catacagcgt aactagaccg tcggcgacca tcgcgtagtc ctcgaggtag
      481 ccgttgaagc gggcccggcc gtccttgtac gagcgcttga gacgcccgtc cacgcgcagt
      541 ttttcgagca ggaaggcggc gtttctctcg gcgacctcgc ggtagtcctc gcggtccagg
      601 acgctggcgg cgaaggcgaa ggagtggagc atcaggccgt tccaggaggc cagaaccttc
      661 tcgtcgcgtc cgggcctggc gcgctcttcg cgggcggcga agagcctgga ccggatccct
      721 tcgacgcgct cccacagctc ctcgggggag agcccgaact ccgcggcgac cacctccggc
      781 ggtcgcggca cgtggagaat gttcctgccc tcgaagttgc cccgttcggt aacgtcccag
      841 aagcgctccg cgaggccggc gtcctcgggg tcgaggactc cccggatctc ctccggcgtc
      901 cacacgtaga acttgccctc ctcgccctcg gagtcggcgt cctcggcgga gtagaagcct
      961 ccctcggggc tcgtcatgtc gcgaacgacg tagtcgaggg tctcctcggc gatgcgccgg
     1021 tagaaggagt cgccggttgc ctggtatgcc tccaggtaga ggcggccgag gagggcgttg
     1081 tcgtagagca tcttttcgaa gtgcggcacc agccagtacc ggtccaccga gtagcgggcg
     1141 aagccgccgc cgagctggtc gtagatgcca ccgttcgcca tcgccctcaa tgtgacctcg
     1201 acgccagaga gcgcgtcccg atctccggtg cggtggtggt ggcggagcag gacctccagg
     1261 ttcatcgcct gcgggaactt cggcgccccg ccgaagcctc cgaagcgccg gtccagctcc
     1321 cccgagaggg acgcggcggc gttgtccagc agcccggttc cgagcacgct cctggggacg
     1381 gcggcgcctg tggccgcctt gaggtactcg cggacgccct cggcgctctt catgacctcg
     1441 tcacggttgt tctcgtaggc gtcggcgagg ctcaggagga cctgctggaa ggagggcagg
     1501 ccgccccgag gggcgggagg aaaatacgtg ccgccgtaga acggggcgcc gtcgggggtg
     1561 aggaagacgg tcatcggcca gccgccgcct cttgtgaggg cctggacggc ggacatgtag
     1621 atcgagtcta tatcaggccg ctcctcgcgg tccactttga tgtttacgaa gtgctcgttc
     1681 attatctgcg ccgtcgcctc gtcttcgaag gactcgcgct ccatcacgtg gcaccagtgg
     1741 cacgccgagt aaccgacgct caagaggatc ggcctgtcct cgttccgagc cctctcgagg
     1801 gcctcctcgc cccacgggta ccagtccacc gggttgtcct tgtgttgcag gaggtaaggg
     1861 ctcgtctcgt tcgccagcct gttggccata tcgtcttacc tcccgcggcg ccggcacggc
     1921 gcccctcgaa acctctgcgc cacaacgtac cacataagag ccgccgttcg gagatttttc
     1981 cgcagtacac gcataattca aagcgaattc atcgccgtct gctctcatgg gtcaaacacc
     2041 ggaaggaaag gagaaagcaa atgtcaacct ctcccgcatc taccgcgacg atccgcccaa
     2101 acgtcgcgag cccgacgttt ggtacgggcg gcggcgggta gaattcattt caaggtaaag
     2161 cgcgagcgag gaggcgaagc ctatgtgcta ctcctacagg gaatacagga tggaggccga
     2221 ggcccgcaga cagcggcagg aagaggagcg caggcgccgc gaggagcagg cgaaaaaggc
     2281 cgagaaagag cgcgcggcca gggagcgcga gctggtaagg gcctgacggg gcctcccaga
     2341 gcccagcgag agcgaaggtc ccgggctgcg gttcggggcc tttgttatat ttgatccggt
     2401 aagaggcaga cggaaggagc ctgacgtgaa cctgcaagag ctcgagaaga cagcccgcgc
     2461 gctagtggcg ccagggaagg gcatcctcgc cgccgacgag agcttcggca ccatcggcaa
     2521 gcgcttcgag gcggtcggca tcgagtcgag cgaggacagc cggcgcgcct acagggagat
     2581 gctcttcaca acccccaaca taggcgagta cctatcgggt gtgatcctct tcgaggagac
     2641 cctcaggcaa aaagcttcgg acggcaggcc gctcgcgaag gtgctcgagg accagggcat
     2701 catccccggc ataaaggtgg acaagagtac cgtgcccatg cccctctccc ctaacgagaa
     2761 gttcacccag gggctcgacg ggttgcacga caggctctcg gagtacaggg agatgggcgc
     2821 acgcttcacc aagtggcggg ctgtgatcac catcggtagc ggcatcccca ccaagagctg
     2881 tgtcgaggcc aacgccgagg cgctcgccct ctacgcgtcg ttcgcccagg accacgacct
     2941 cgtccctatc gtcgagcccg aggtcctcat agacggagac cactccatcg agcagtccta
     3001 cgaggccacg ttgtggacac tccacgagac cttcgacgag atccacgagc acggcgtcga
     3061 gctctcggcg ctgctcctga agccgaacat ggtcatctcg ggtaaggaag cctccgatca
     3121 ggccaacgtc gaagaggtag cccgctacac ggtcgagtgc ctgcttcgca ccgtgccgcc
     3181 tgccgtgccc ggtatcgtct tcctctcggg aggccagacc gacctgcagg ccacgcagca
     3241 cctgaacgcg atgaacacca tttacgaggg cctgccctgg gagctctcct tctcctacgc
     3301 ccgcgcctta caggggcagc cgatggagat ctggggcggc aaggccgaga acgtcgagcg
     3361 ggcccagaag atcttccacc acagggcgag gatgaacagc gcagcccgcg acggcagata
     3421 ctccgaggag atggagcagg aagcggcgta aaatagcacg cctcctccct tcggtcggct
     3481 tctcagcacg ccctcttcga gggcttgtca gccaggatgg cgcacttcgt cgctccgcag
     3541 cgcttgctag agctattgcc agaaggccgt ctcgtaacac gaaactgggc atgaggcgag
     3601 cggcaataag ctgaaatgct gacaagcgga ggccgccagg ccggagcgtg ctgacaagct
     3661 gcctgaaagg cagcgtgctg acaagcctgc aaa
//
LOCUS       DASTUV010000242         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1590480, whole genome shotgun sequence.
ACCESSION   DASTUV010000242 DASTUV010000000
VERSION     DASTUV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590480"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1211)
                     /locus_tag="VFT03_04860"
     CDS             complement(312..1211)
                     /locus_tag="VFT03_04860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU4847507.1"
                     /translation="MKIGVQIPNNSANAQEIGLTKMGRLAEDAGADSLWVNDHLLMVE
                     GGRSPYPFTPDGAIPWPMDMPWYECLTAASFLAATTSRCRIGTSVLILPQRNVLELAK
                     TAATLDALSGGRFVFGLGAGWFAEEIEALGYSFRTRGQRTDEMIEVLRRCWTGRPRPF
                     EGKEIVVPEGLVFEPRPAQPGGPPILVGGMSRAALKRAALRGDGWLAFQRGELDPEKL
                     AELLETLENLRGEFGRLDQPFSSVMRLHTPPEMAPRLSEFAIQVQELGFDELIITPSW
                     DDLAEVQRLINQVKGTVSASGAS"
     gene            1615..1788
                     /locus_tag="VFT03_04865"
     CDS             1615..1788
                     /locus_tag="VFT03_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847508.1"
                     /translation="MLQYTNFKLALLAAADTEPGTVYSHPFEEGLYVVPETHAEEQRL
                     VDAGVVLAEVEPV"
     gene            complement(1845..>2178)
                     /locus_tag="VFT03_04870"
     CDS             complement(1845..>2178)
                     /locus_tag="VFT03_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU4847509.1"
                     /translation="ERYSPCHPGSRGLLRRCPPGELRRVMVVKFWLHFGVDAFDQEEM
                     LAVTYPLPDGLGWEHVEALLRPLVQSRRLVGLSVADFVPDKDPDGRYARRLVELIAGL
                     LAPQPEKT"
BASE COUNT          435 a          656 c          681 g          406 t
ORIGIN      
        1 aggggcacat gagcgacgac gatctagccg aagcccttgc cgagctcgat ggaactcgcg
       61 ccactgcaga gagggagctg aaatccatac ggggacgtaa ggcaacgctg gaggagcttg
      121 agcgggaccg ggacgcgctt ctcgaatcct acgccggaat gatacccgag gcgctcgatt
      181 ccttggctcc agaggagcgc tctcagatat atgggatgct caggctcaat gttgatgtat
      241 ttgcggacgg tacgatgcag gcacaagggg ttctgggcgt agtgccacct gtttgtgata
      301 acggacttgc atcaggatgc gccgctagcc gaaaccgtac ctttgacctg gtttatcagt
      361 ctctgcactt cggcgaggtc atcccaggag ggagttatta tcagctcgtc gaaaccgagt
      421 tcttgcacct ggatggcgaa ctccgacagt cggggtgcca tctcgggagg agtgtgcaag
      481 cgcatgacgc tgctgaacgg ttggtcgagt cgcccgaatt ccccgcgaag gttctcgagt
      541 gtctcgagca gctcggcgag tttttcaggg tcgagctctc ctctttggaa ggcgagccag
      601 ccatcgccgc ggagcgcagc ccgctttagt gccgcccggc tcatcccgcc gacgaggatg
      661 ggaggaccgc ccggctgggc gggtcgcggc tcgaacacta gaccctccgg cacgacgatc
      721 tccttaccct cgaagggccg gggtcttccc gtccagcacc gtctcaggac ctcgatcatc
      781 tcgtcggtac gttgcccgcg ggtccggaag ctgtagccga gcgcctcgat ctcctcggca
      841 aaccatccgg cacctaaccc aaagacaaag cgccctccgc tcaaagcgtc caaggtggcg
      901 gccgtcttcg ccagctccaa gacgttgcgc tggggaagga taaggaccga ggtgccgatc
      961 ctgcacctcg aggtggtggc ggccaggaag gaagcagcag taaggcattc gtaccagggc
     1021 atatccattg gccacgggat agcgccgtcc ggggtgaacg ggtagggcga gcgtccacct
     1081 tcgaccatca gaaggtggtc gttgacccag aggctgtcgg cgccggcgtc ttctgccagc
     1141 cgccccatct tcgtgagacc tatctcttgg gcattagcac tgttgttggg gatctgcaca
     1201 ccaatcttca tggagatacc tcagtgtcgc ccacctgccg actgccttcg ctagccgaga
     1261 tgccattcta ctcctctcat ggctggcggg actttctcag ttcgccgcat acacacacac
     1321 agacgagttt ggccttagtg atccctaaca cgtaagagta ggcgggcctt tatgcctcgt
     1381 cttgatagcc tcgaacatga ttcaaggacc gctactcgcc ggtgagcgca aaaattggtt
     1441 tcaagttgcg caggatcact gggtaggcaa ggggccaggg cagcgcattg gtttgaacga
     1501 catttctatg cgacatgcgt acccggctgg gtggggcgtg gagaaacaac ccgggccgcc
     1561 ggacgtatcc gtacctatcc aggcgtttgg acaccgacgc agagaggaga gacgatgctg
     1621 cagtacacca acttcaaact ggccctcttg gccgccgcgg ataccgagcc cgggacggtg
     1681 tacagccatc cgttcgagga gggcctctac gtcgtcccag agacgcatgc cgaggaacag
     1741 cggctggtag acgccggcgt agtgctcgcc gaggtggagc ccgtttagcg gcggtcctat
     1801 tacctcgaag ggggcgaccg gccagttaga ggatgcgagc ccactcacgt cttttccggc
     1861 tgtggtgcga gcaggccggc gatgagctcg actaggcggc gcgcgtacct cccgtccggg
     1921 tctttgtcag ggacgaagtc ggcgaccgag aggccaacga gcctccgaga ctgtaccagc
     1981 ggtcgcaaca gtgcctccac gtgctcccaa ccgagcccgt cgggcaacgg gtaggtgact
     2041 gccagcatct cttcttggtc gaaggcgtcc acgccgaaat gcaaccagaa cttgacgacc
     2101 atcacgcgcc tcagctcgcc aggaggacac cgccgaagca gaccgcgaga cccaggatgg
     2161 caaggagagt aacgctct
//
LOCUS       DASTUV010000243         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_191184, whole genome shotgun sequence.
ACCESSION   DASTUV010000243 DASTUV010000000
VERSION     DASTUV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191184"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..842)
                     /locus_tag="VFT03_04875"
     CDS             complement(135..842)
                     /locus_tag="VFT03_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU4847510.1"
                     /translation="MADNVDLQARPREERGKNAARRLRASGMLPAVLYGDGDGVSTAL
                     AVPDRVVDYTLQHIGDNALYDIDLGAGGSTARIVDVHRDPVSGRLLHVDFAPVDMQRR
                     IEVTVPLHVVGEAPGTEEGGVLQQVAYEVQIETLPGDIPQELILDVSSLGMNENLTLA
                     DLRLPEGITLVSDPEEVAAIVTAPTEITEEELEAAGVVEEEPEEVVEEGEEAPEAAEG
                     EEEPEEGEEPENQNQQA"
     gene            complement(867..1865)
                     /locus_tag="VFT03_04880"
     CDS             complement(867..1865)
                     /locus_tag="VFT03_04880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU4847511.1"
                     /translation="MATRNGHLTQTTEKRLMLFSGEGYPDLADRIADRLDIELGGVEL
                     RQFSAGEVYARYLDSVRGADVFIVQTLGQPVNRNLMQLLVMIDAAKRASAKRITAVIP
                     WYAYSRQDRKTKPREPITARLVANMIEVAGAERVMTMDLHVGQIEGFFSFPVDHLTAM
                     HTFVDHFVEEGFRDADDVVVVAPDTGEVKVAKRLADHLGLPWAIVNKMRREVQQSEVT
                     HVIGDVRDKRVIMIDDLIDTAGTLCNAAEKLIEEGATEVYAAATHAVFSGPAYKRIEA
                     SPLKEVVVTDSLPLKEDEPRSKIRTLTIAPILASTIRNVFTDESVSAVFMGENQLF"
     gene            complement(1870..3267)
                     /gene="glmU"
                     /locus_tag="VFT03_04885"
     CDS             complement(1870..3267)
                     /gene="glmU"
                     /locus_tag="VFT03_04885"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU4847512.1"
                     /translation="MSHAETLPQTFAVVLAAGKGTRMKSNRAKVLHTLCGVPMVNYVI
                     GALKSLAPERLIVVVGHQADLVRGVLPGDAEAVLQREQLGTGDAVRVALEALEGEEGV
                     LLVVNGDGPLISEMTLGELLERHRSAGVGATVLVAELEEPRGLGRVVEEAGVVRIVEE
                     RDATDEERAIRLVNLGLYAFELSEIRDAIGKVGVDNGQGELYLTDVLEIIGRRSRAVT
                     YRLKDLEEANLVNDRSQLARAEEILRRRILDAHMREGVTVRDPVSTHIEASVQIGRDT
                     VILPGSFLRGTTKIGSDCVIGPSADLLDTRVGDGARVEHSVGRGAEVGEGAAVGPYAF
                     LRPGTVLGPDAKVGAYCEVKNTRVGRGSKVPHLSYVGDAEIGEDANLGAGTITANYDG
                     VNKHRTTIGDGAFTGVNTNLIAPVTIGDGAYLGAASVVNKDIPPGKLAVGMPARVIRD
                     APGRTKEGPRDETEG"
     gene            complement(3313..3383)
                     /locus_tag="VFT03_04890"
     tRNA            complement(3313..3383)
                     /locus_tag="VFT03_04890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3349..3351),aa:Gln,seq:ttg)
     gene            complement(3541..3942)
                     /locus_tag="VFT03_04895"
     CDS             complement(3541..3942)
                     /locus_tag="VFT03_04895"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU4847513.1"
                     /translation="MNDSFDSDTLRLIDGTREVRIETSRDEDAPTHRTTIWVVTVDGT
                     VFVRSVRGGKGRWYTEVSANPAAALHVGDERLPVRAVPVTDDATIAAVSEAYREKYGR
                     TSPGSTRAMMQPETLPTTLGLEPAREGASPN"
     gene            complement(4026..>4349)
                     /locus_tag="VFT03_04900"
     CDS             complement(4026..>4349)
                     /locus_tag="VFT03_04900"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847514.1"
                     /translation="AYDERDSVVIACADTVVAALHSRSLTELAEALGNDLEPVTTGLV
                     PEVAAHKREMLRAGALGAAMTGTGTAIYGIFDAESGDSPAAVASLRAPFLGEFEPAGA
                     GVSML"
BASE COUNT          661 a         1522 c         1328 g          838 t
ORIGIN      
        1 acgaggaagc cgacgttgtg gcgggtgcgc tcgtaggagc ggccagggtt gccgaggcca
       61 acgacgatgg gggaccgtga aggtcccccg tcgcctcgac ctctaagcct gtcgaagata
      121 gagcgtatgg gttcctacgc ctgctggttc tggttctcgg gctcctcgcc ctcttcgggc
      181 tcctcttcgc cctcggcagc ttcgggagct tcctcgccct cctcgacaac ctcctcgggc
      241 tcctcctcga cgacgccggc ggcttcgagt tcctcctcgg tgatctccgt cggcgccgtg
      301 acgatggcgg cgacctcctc ggggtcggag acgagggtaa tgccttcggg gagcctcagg
      361 tccgccaggg tgaggttctc gttcatcccc agggaggaga cgtcgaggat gagctcctgc
      421 ggtatgtcgc cggggagcgt ctcgatctgg acctcgtagg cgacctgctg cagcacgccg
      481 ccctcctcgg tgccgggcgc ctcgccgacg acgtggagcg gcacggtgac ctcgatccgc
      541 cgctgcatgt caacaggggc gaagtccacg tggaggaggc ggcccgaaac ggggtcgcga
      601 tgcacgtcca cgatgcgcgc cgtcgagccg cccgccccga gatctatgtc gtagagcgcg
      661 ttgtccccaa tgtgctggag ggtatagtcc acgaccctgt cagggacggc gagcgccgtg
      721 ctcactccgt ccccgtcgcc gtagaggacg gccgggagca tcccggaagc cctcaagcgc
      781 cgggccgcgt tcttgccgcg ctcctcgcgt ggtcttgcct gcaggtctac gttgtctgcc
      841 atcttcttat ctcccagaga gtcttgctag aaaagctggt tctcgcccat gaagaccgcg
      901 ctcaccgatt cgtcagtaaa gacgttcctt atagtgctcg cgagtatcgg cgcgatcgta
      961 agcgtgcgga ttttagaccg cggctcgtcc tccttcaagg gtaacgagtc ggtgacgacg
     1021 acctccttga ggggtgaggc ctcgatccgc ttgtaggccg gacccgagaa taccgcgtgc
     1081 gtcgcagccg cgtagacctc cgtcgcgccc tcctctatca gcttctccgc ggcgttacac
     1141 agagttccgg cggtgtctat caggtcgtct atcatgatca cacgcttgtc cctcacgtcg
     1201 ccgatgacgt gggtgacctc ggattgctgt acctcgcggc gcatcttgtt cacgatggcc
     1261 cacggcagcc caaggtggtc agccagcctc ttcgctacct tgacctcgcc cgtgtcgggc
     1321 gcgacgacca cgacgtcgtc ggcgtcgcgg aagccctcct cgacgaagtg gtcgacgaag
     1381 gtgtgcatgg cggtcaggtg gtcaacaggg aagctgaaga aaccctcgat ctggccgacg
     1441 tgcaggtcca tcgtcatcac ccgctctgcg cctgcaacct cgatcatgtt cgccacaaga
     1501 cgcgccgtta tgggctcgcg cggcttggtt ttcctgtcct ggcgagagta cgcgtaccac
     1561 ggtatgaccg ccgtgatcct cttggccgag gccctcttgg cagcgtcgat catgacgagg
     1621 agttgcatca ggttgcggtt aaccggctgg cccagcgtct gcacgataaa cacgtcggct
     1681 ccccgcacgg agtcgaggta gcgggcgtac acctcaccgg cggagaactg cctcagctcc
     1741 acgccaccga gctctatatc cagccggtcg gctatccggt cggcgagatc tgggtagccc
     1801 tcccctgaga agagcattag ccgcttctcg gttgtctgtg tcaggtggcc gttccgcgtc
     1861 gccaaaattc tagccctccg tctcgtcgcg tggtccttcc ttcgtcctgc cgggagcgtc
     1921 gcggatcacg cgcgccggca tcccgaccgc gagcttgcca ggcgggatgt ccttgttgac
     1981 gacgctcgct gctcccagat atgcgccgtc ccctattgta accggtgcga taaggttcgt
     2041 gttcacgccg gtgaacgccc catcccctat ggttgtcctg tgcttgttta cgccatcgta
     2101 gttggcggtg atggtgccgg cgcccaggtt cgcgtcctcc ccgatctccg cgtcgcctac
     2161 gtaggagagg tgggggacct tgctccccct ccctacgcgc gtgttcttga cctcgcagta
     2221 tgcgcccact ttggcatcag ggccaagcac cgttccaggc cgcaagaaag cgtagggtcc
     2281 gacggccgca ccctctccaa cctcggcgcc ccggcctaca gagtgctcga cgcgcgcccc
     2341 gtcgcccacc cgtgtgtcca ggaggtcggc ggagggtcct atcacgcagt ccgagcctat
     2401 cttcgtggtt ccgcgcaaga agctgccggg caggatcacc gtgtcgcgcc cgatctgtac
     2461 gctagcctcg atatgtgtac tcaccgggtc ccgcaccgtg accccttctc tcatgtgggc
     2521 atccaggatg cgacgcctca ggatctcctc cgcccgcgcg agctgcgagc ggtcgttgac
     2581 caggttggcc tcctccaggt ccttgagcct gtaggtcacg gcccggctcc tgcgaccgat
     2641 gatctccagc acgtccgtaa ggtacagttc gccctggccg ttgtccacgc ccaccttccc
     2701 tatggcgtcc cggatctccg agagctcgaa cgcgtacagg cccaggttga ccagtcttat
     2761 ggcccgctcc tcgtcggtcg cgtcgcgctc ctcgacgatc ctcaccacgc cggcttcctc
     2821 gacgacccgc cccaggcctc ggggttcctc gagctccgcg accagcacgg tcgcccccac
     2881 cccggcagac cggtgcctct cgagcagctc tcccagcgtc atctccgaga tgagcggccc
     2941 gtcgccgttg accactagga gcacaccctc ctcgccttcg agagcctcca gcgccacgcg
     3001 cactgcgtcc cccgtgccga gctgctcgcg ctggagtacg gcctccgcgt ctccgggaag
     3061 cacacccctg acgaggtcgg cctggtggcc cacgacgacg atgagcctct ccggcgccag
     3121 ggacttcagg gcccctatca cgtagttgac catcgggacg ccgcagagcg tgtgcagtac
     3181 cttcgcccgg ttcgacttca tcctggtacc cttgccagcc gccagaacca cggcgaaagt
     3241 ctgcggcaga gtttctgcgt gggacatggg ctctcagggt ctccttggga tagcttcgct
     3301 cacgcctgcg ggctggggcg gcaggattcg aacctgcgat cccgggacca aaacccggtg
     3361 ccttgccact tggccacgcc ccagtatcct cttatgcccg cgccgccgcg attataccgg
     3421 aggctcccgc gactatcagg tctgcgaaac catccgattg accagccagt tgccttcacg
     3481 cgtccgaaag atggcgtagt aatgttcctg gatcacgaag cgcggcaacg gttacggccc
     3541 tcagttgggc gaagcccctt ctcgagcggg ctcgagcccc agtgtggtcg gcagggtttc
     3601 gggctgcatc atcgcccggg tcgagcccgg cgaggtacgt ccgtacttct ccctgtaggc
     3661 ctcgctgacc gccgcgatag tcgcatcatc cgtcaccgga acggcgcgca cagggaggcg
     3721 ctcgtcgcca acgtgcagcg cggccgccgg attcgccgac acttctgtgt accagcgacc
     3781 cttcccgccg cgcaccgacc gcacgaagac ggtcccgtcc acggtcacca cccagatcgt
     3841 cgtgcggtgc gtcggggcgt cctcgtcccg gctggtctcg atccggacct ccctggtccc
     3901 gtcgatcagc cgcaaggtat cggagtcgaa ggaatcgttc accgcaggct cctttttcgg
     3961 cgctctatcg ttccatccta ttctaccgcc gaagatgccg ggaactacga gcactcgcgg
     4021 ttcggctaga gcatcgatac cccggccccg gcgggctcga actcacccag gaaaggagcg
     4081 cggagcgacg ctacggcggc cggtgaatca ccactctcgg cgtcgaagat cccgtatatg
     4141 gcggtgcccg tgcccgtcat cgcggcgccg agcgcccccg ctcgcagcat ctcccgcttg
     4201 tgagctgcca cctcgggcac gagccccgtg gtgaccggct cgaggtcatt gccgagggcc
     4261 tcggcgagct ctgtgaggct gcgcgaatgg agcgccgcaa cgaccgtatc tgcgcaggcg
     4321 attactactg agtcgcgctc gtcgtaggc
//
LOCUS       DASTUV010000244         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_708653, whole genome shotgun sequence.
ACCESSION   DASTUV010000244 DASTUV010000000
VERSION     DASTUV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708653"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFT03_04905"
     CDS             <1..270
                     /locus_tag="VFT03_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847515.1"
                     /translation="QERRSVIREGRYRTPDRRGQTQMREVIRKRLKECPVRVQRSEVE
                     AMGAEIGLDGVAAARLFDRLKGVSWRGDYIRSDNGWVAAWVREVN"
     gene            408..1793
                     /locus_tag="VFT03_04910"
     CDS             408..1793
                     /locus_tag="VFT03_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU4847516.1"
                     /translation="MCSRARAVEGGEGLEASVGTLLGGRYRIQSTLGSGGMAVVYKAE
                     DAILGRTVALKTLHRRYAEVPSFRQRFKQEARAMACLDHENVVKVYDISHEGEVPFIV
                     AECVAGRDIGKLLAGHRSGTLNEKFANRVAAQLLRALSYAHRRGVIHRDIKPSNILVT
                     AGGTVKVADFGIARIVEEEDGVGSPGEIVGSARYMAPEQLMGKEATPRSDIYSVGVLL
                     YHCLTGRLPFSGGLRSLARQHIHEDPTPPRKLNPKISPQTEAVILKALAKDPDHRFPS
                     AVAMLDAIDVEPLPRIERTAEVPRSGKSRRWLVLASTLVVLVALAGGGALASGLGPAG
                     LPLLDRSGGSDALSRAEHPEAGEPPAAPESSREPGSEGSASRDSARSSAPARRDLVRV
                     PDVRAFYDYFAEETLVGMGFKVRFVYGYQEGYASRGVTWATDPAIGTLAPRGSKITVY
                     ATPEDYRQPQL"
     gene            complement(1790..2236)
                     /locus_tag="VFT03_04915"
     CDS             complement(1790..2236)
                     /locus_tag="VFT03_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEU4847517.1"
                     /translation="MHEDQNYGRLSDDTTGGSYPSGGSMSPQDERTWCVLAHLSVLAG
                     LVGLMPFGALIIWLLFKDRSQRVGFHALQALWYQVAWLIILVAGWFVTFVLSFVLVGL
                     LFIPVMLVASLVPFVHGCYAAYRVSQGEDYRYPFIADRIEGAGRTL"
     gene            complement(2266..2820)
                     /locus_tag="VFT03_04920"
     CDS             complement(2266..2820)
                     /locus_tag="VFT03_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847518.1"
                     /translation="MGRMKPAPQDPTLAPWMSLQERLAGYLAARVRGLLASDFVLSGR
                     EGEELGRLRVYGRRGADLEAGIITAAIERGAGSRYTMVDSDGTVILEGTAGSGDVSEV
                     RRGDRVYEVWLALLRNAAVVRSSGGAEVARVAGGLTNRSYEAFFDAGEEGALPLAVFL
                     LYCIIALRRRAFLMGTERGDASSA"
BASE COUNT          559 a         1009 c         1013 g          460 t
ORIGIN      
        1 caggaaagga gatcggtgat cagagaggga aggtaccgga ctcccgacag gcggggccag
       61 acccagatgc gcgaggtcat caggaagagg ctcaaagagt gtccggtccg cgtccagcgg
      121 agcgaagtgg aggcgatggg ggcggagata gggctcgacg gcgtcgcggc cgcccggctc
      181 ttcgaccggc tcaagggcgt ctcctggcgg ggcgactaca tcaggtccga caacggttgg
      241 gtcgccgcct gggtcaggga agtcaactag acccgccccg gcgagctgtt tcgccgccga
      301 acccggacac aacatgtggc gcccggtttc ggtgggctcc atcatgttgg gcgccatcac
      361 gctcgcacag cggcgagctt acctggggcg ccttcggggc tatataattg tgttctaggg
      421 cacgcgcggt cgaaggtggt gagggactgg aagcctcggt cgggaccctc ctggggggac
      481 gttacaggat acagagcacg ctgggctccg gcgggatggc ggtcgtctac aaagcggagg
      541 acgccatcct cgggcgtacc gtcgcgctca agacgcttca caggcgctac gccgaggtac
      601 cctccttccg ccagcgcttc aagcaggagg cgcgggctat ggcctgcctc gaccacgaga
      661 acgtcgtcaa ggtctatgac atctcccatg agggcgaggt gcctttcatc gtggccgaat
      721 gcgtcgccgg cagggacatc ggcaagctcc tcgccgggca ccgcagcgga accctcaacg
      781 agaagttcgc caacagggtg gctgcgcagc tcctgagggc gctctcctac gcccacaggc
      841 ggggcgtcat ccacagggac ataaagccat ccaacatcct ggtaacggcg ggcgggaccg
      901 tcaaggtggc cgacttcggc atcgcccgca tcgtcgaaga ggaagacggg gttggaagtc
      961 cgggcgaaat cgtgggcagc gcccgctaca tggccccgga gcagctaatg ggcaaggagg
     1021 cgacgccgcg cagcgacatc tactccgtcg gcgtcctgct ctaccactgc ctcacgggca
     1081 ggctgccctt ctcgggcggc ttacgttccc tcgcccggca gcacatccac gaagatccca
     1141 cgccaccgcg caagctaaac ccgaagattt cgcctcagac ggaggcggta atcctgaagg
     1201 cgctggccaa ggatccggac caccgcttcc cctcggcggt cgcgatgctc gacgccatag
     1261 acgtcgagcc tctcccgagg atcgagagga cggccgaggt tcctcgctcg ggcaagagcc
     1321 gaaggtggct cgtgctcgcc tcgacgctcg tcgtgctggt ggcgctggcc ggcggtggcg
     1381 cccttgcctc cggcctgggg cccgcggggc tgccgttgct ggatcggtcc ggagggtcgg
     1441 atgccctgag ccgggccgag cacccagagg ccggagaacc cccggcggcg cccgagtcgt
     1501 cccgcgaacc agggtctgag gggtcggcct cccgagactc cgccaggtcg tcggcgcctg
     1561 cccgccgcga cctcgtccgc gtgccggacg tgcgggcctt ctacgactac ttcgccgagg
     1621 agaccctggt tggaatgggc tttaaagtca ggttcgtcta cggctaccag gagggctacg
     1681 ccagccgcgg cgtgacgtgg gccacggatc cagccatcgg aaccctcgcg ccgcggggat
     1741 ccaagatcac ggtctacgcc acccccgagg actatcgcca gccgcagctt tagagggtcc
     1801 tgccggcgcc ttctatcctg tcggcgatga atgggtaacg gtagtcctcg ccctggctca
     1861 ccctgtaggc cgcgtagcac ccgtggacga aaggcaccag ggaggcgacg agcatcaccg
     1921 gtatgaaaag cagcccgacc agcacgaaac tcaggacgaa cgtcacgaac cagcccgcga
     1981 cgaggatgat gagccaggcc acctggtacc agagcgcttg cagggcgtga aagcctaccc
     2041 gctgcgacct gtctttgaag agcaaccaga tgatgagggc gccgaacggc ataagaccaa
     2101 cgagacccgc gagcacgctc aggtgcgcca gcacgcacca agtgcgctcg tcctggggac
     2161 tcatcgagcc gccggagggg taggacccgc ccgtcgtgtc gtccgagagc cttccgtagt
     2221 tctgatcttc gtgcacaatc tctccttctc tgggacgttg ctcccctaag cgctcgaggc
     2281 atcgcccctc tccgtgccca taaggaaagc ccggcggcgt agtgcgatga tacagtagag
     2341 gaggaatacg gccaggggca gcgccccttc ctccccggcg tcgaagaagg cctcgtagct
     2401 ccggttggtg aggccgcccg cgacgcgcgc cacctcagca cctcctgagg agcggacgac
     2461 ggcggcgttg cgcaagaggg cgagccagac ctcgtagacg cggtccccgc gcctcacctc
     2521 cgagacatca cccgatccag cggtcccctc taggatgacc gtcccgtcgc tgtccaccat
     2581 cgtgtagcgc gaacccgcac cacgctcgat tgccgccgtt ataatcccgg cttcgaggtc
     2641 cgcgcctcga cgcccgtaca cgcgcagcct gccgagctcc tcgccttccc ggcccgagag
     2701 aacgaaatcc gacgcgagga gccctcggac ccgggccgcg aggtagccgg cgaggcgctc
     2761 ctggaggctc atccagggag cgagcgtggg gtcctgcggc gcgggcttca tcctacccat
     2821 aattacgcgc cggaagcggt ggagtttcgt aggtcgagcg ggtaaattac gtgtgatgca
     2881 acgaagagag cttggagcgt catggagcta aaagacacag ggcagctcag aaacgagatc
     2941 ctgcagacct ggctcaagag cgcctgggtc tatcccgtgc agggacccga ggagaggacc
     3001 taccttcgcc tgacccccgg cggccggctc aagatgcgcc g
//
LOCUS       DASTUV010000245         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_939752, whole genome shotgun sequence.
ACCESSION   DASTUV010000245 DASTUV010000000
VERSION     DASTUV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939752"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFT03_04925"
     CDS             <1..729
                     /locus_tag="VFT03_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU4847519.1"
                     /translation="IANGGRRVEPTMISRVVSDEGKEGEKVLYEAPDHPEGKQVIDPE
                     IAHEATEIMIGDVTEGIAEDASLGNRPVAGKTGTSENFFDAWFVGFTPQLLTGIWMGY
                     ADGGQTLEYDLEYARELNGLSGGITPAQIWKTYMEQMLEGQPIEQFEGVEMPTQEHTT
                     PTDAVTPGTEPSGAAGPRVVEPLPAGVLGDRPTNVEPSSASPSSAAPSSVPPSSAAPS
                     SAAPSSASPSSANPSRRPGGRGLR"
     gene            complement(689..1327)
                     /locus_tag="VFT03_04930"
     CDS             complement(689..1327)
                     /locus_tag="VFT03_04930"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU4847520.1"
                     /translation="MRPHRRAGVAKDILKRDFYDRDPRQVAVDLLGCVLSHRTSKGLA
                     SGIIVEAEAYRPEDPACHAYNGPTMRNRTMFGVPGLAYVYLSYGIHRLLNVVCEGEGV
                     GSAVLIRALRPREGRELMARRRGRDSSLCNGPGRLTQALGIDLTRDGHDLTGGDLRIS
                     WGDTPADIVATTRIGITRGTGLPWRYLVYGDENVSVRPRTIEGRGLPASWRG"
     gene            complement(1285..1644)
                     /locus_tag="VFT03_04935"
     CDS             complement(1285..1644)
                     /locus_tag="VFT03_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847521.1"
                     /translation="MKVVPDRIVPLGFGKYVRADRIVGLIPIEEDRGSGHRTLVYVEG
                     LPEPLTAGRTEATILRDMVGTESGSVELLRELQQQIGQVRPLLKSSIRSEAGLDLDEL
                     SRRISDLTGEPESPRIF"
     gene            complement(1756..2478)
                     /locus_tag="VFT03_04940"
     CDS             complement(1756..2478)
                     /locus_tag="VFT03_04940"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEU4847522.1"
                     /translation="MGGLILVQRLVPMSVRQRQNDVAGFIYAVLGVAYAVLLGLMLVA
                     VWEQWEAAKIIADDEANEVAEIFWIAHQLPQPEGRRIQELARSYSRVVVEEEWPLMEK
                     GKASPRAWTTLHDLRDSVQSFHPTTDAQLALYEEALDRVHELSDARRERLLAADEGLP
                     VILWVVLIIGGVLVVGFTYLFGMENNLTHILMVATLALIISLVLFTVAALNYPFRGDV
                     HIGPEAFEQVLGSFESSDLSDL"
     gene            2886..3434
                     /locus_tag="VFT03_04945"
     CDS             2886..3434
                     /locus_tag="VFT03_04945"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU4847523.1"
                     /translation="MKEKRLVGERVELRRHSPVNYRLYGAWYADPEIWRLTSWSAAPL
                     SPATVERLFEGREHSPTDDSFAIHLKDEREPIGVLSLMNISEANASAELSIIVGHPED
                     RHHGYGAEAIATLLDYAFGERGLNRVGLSVFEFNEDAISAYAKLGFREEGRLRRALKR
                     DGAFHDAILMSVLKDEWVPRGP"
     gene            complement(3449..>3964)
                     /locus_tag="VFT03_04950"
     CDS             complement(3449..>3964)
                     /locus_tag="VFT03_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847524.1"
                     /translation="GFFEAKGFVERLVPGARFEPAERPFLHPGRSAVVLVEGREVGWV
                     GELHPEVAERFELGGWPVAALELDLTPVEPDPEPSFEAFVNVPAVSRDLAVVVASSVP
                     VGEMLAAVESLGSPILAETRVFDVYEGRQVPEGNKSVALGFTFQAEETLTDEAVNDEL
                     RRIATRLEDEF"
BASE COUNT          753 a         1328 c         1225 g          658 t
ORIGIN      
        1 atagccaacg gcgggcgccg ggtggagccg accatgatct ccagagtggt aagcgacgag
       61 ggtaaagagg gagagaaggt tctctacgag gcgccagacc atccagaggg gaagcaggta
      121 atagatcccg agatagccca cgaggctacc gagataatga ttggcgacgt caccgaaggc
      181 atagccgagg acgcctcgct cggcaaccgg cccgtcgccg gcaagaccgg gacgagcgag
      241 aacttcttcg acgcgtggtt cgtcggcttc accccgcaac tgctaaccgg catctggatg
      301 gggtacgccg atggcggcca gaccctcgag tacgatctgg agtacgcccg cgagctgaac
      361 ggcctctccg gcggtatcac cccggctcag atctggaaga cgtacatgga gcagatgctc
      421 gaaggtcaac ccatcgagca gttcgagggc gtggagatgc ccacacagga gcacaccacc
      481 cccacagacg ccgtaacacc gggaaccgaa ccatccggcg ccgccggtcc gagggtcgtc
      541 gaacctctcc ccgccggagt tctgggcgac cgccccacga acgtcgaacc gtcgagcgcg
      601 agtccttcca gcgccgcccc ttcgagcgtg cctccttcga gtgccgcgcc atcgagcgcc
      661 gcgccatcga gcgcgagtcc ttcgagcgct aacccctcca ggaggccggg aggccgcggc
      721 cttcgatagt cctgggccgc acggagacgt tctcgtcccc gtaaaccagg taacgccagg
      781 gtagcccggt gccgcgcgtg atccctatgc gggtggtggc cacgatgtcc gcaggggtat
      841 cgccccacga tatcctgaga tcaccacccg taaggtcgtg gccgtccctg gtgaggtcga
      901 tgccgagagc ctgggtcagc ctccccggtc cgttgcacag gctcgagtcc ctgcctcgtc
      961 gccgggccat gagctcgcgg ccctcgcgcg ggcgcagcgc ccggatcagg acggcgctcc
     1021 ctaccccctc gccctcgcac actacgttga gcaaacgatg gatcccgtac gacaggtaga
     1081 cgtaggcgag acccggcacg ccgaacatgg tgcggttgcg catcgtcggg ccgttgtagg
     1141 cgtggcaggc ggggtcctcg ggccggtagg cttcggcctc cacgatgata cccgaggcga
     1201 ggcccttgga ggttctgtga gagagcacgc agccgaggag gtcgaccgcg acctgcctgg
     1261 ggtcccggtc gtagaaatct ctcttcagaa tatccttggc gactccggct cgccggtgag
     1321 gtcggatatg cgccgagaga gctcgtccag gtcgagcccg gcctcggagc ggatcgagga
     1381 cttgagaagg ggacggacct ggcctatctg ctgctgcaac tcgcgcagca gctcgaccga
     1441 accactctcc gtgcctacca tgtcccgcag gatggtggcc tccgtccggc ccgcggtcag
     1501 cggctcgggc agaccctcga cgtagacgag ggtgcggtgg ccggagcccc ggtcctcttc
     1561 tatggggatg aggccaacta tcctgtcggc gcggacgtac ttgccgaacc cgagcggaac
     1621 gatgcgatca ggcacgactt tcatagtgat aaaagctcca tttcacaaac atttataaca
     1681 tccccgctta ctcgtagcga cccgtgtctc gcccggtctg ctccggctgc ggtcagcatg
     1741 gcggaaagcc cgctgtcaga ggtcgctgag atcgctgctc tcgaaacttc ctagtacctg
     1801 ctcgaaggcc tccggaccga tatgcacatc gcccctgaac gggtagttga gggcggccac
     1861 cgtaaagagc accagggaga tgatcagcgc cagtgtcgcc accatcaata tgtgggttag
     1921 attgttctcc atcccgaaga ggtaggtaaa gcctacgacc aatactcccc cgattatcag
     1981 caccacccat aggatcacgg gcaagccctc gtcggcggcc agaagtcgtt ccctcctagc
     2041 atcggacagc tcgtgcaccc gatcgagcgc ctcctcgtag agcgccagct gggcatcggt
     2101 ggtcggatgg aagctctgga cgctgtccct gaggtcgtgg agcgtggtcc atgccctcgg
     2161 gctcgccttg cccttctcca tcagcggcca ctcctcctct accaccaccc gcgagtagga
     2221 gcgggcgagc tcctggatac gacgaccctc gggttggggc agctggtggg cgatccagaa
     2281 aatctctgct acctcgttgg cctcgtcgtc cgcgatgatc ttggccgcct cccactgttc
     2341 ccatacagcg acgagcatga gcccgagcag cacggcgtat gcgacgccca ggacagcgta
     2401 gatgaatccc gccacgtcgt tttgccgttg cctgacgctc atgggcacca ggcgctggac
     2461 caggatcagc ccacccaccg cgaacaccac gaacagacat atgagtaaga caccactgag
     2521 gatagagatc ggcgcacctc catctgttcg cttatgcaag ccggtaggat caagcatacg
     2581 ctgcaaggag gatcgtatgc aaacgccgcc acagtctcgc catgcatccg ttagcggcga
     2641 ggttctgagc ctgacttctc gtatacaacc cacctgcctt tgcgcaagtc cgccgccctc
     2701 caccgtggaa tgcccgccgc tgaattcccg cgtcctatgc ccgcttcact tccaaccgct
     2761 cgatgccatc ccgggtcagt acagcgcgga accggagtga gccggtggta tttagagata
     2821 acaaattgca tggtacgcat atatcgtcat agcggcactc ttgggaagcg gctatattct
     2881 ctttcgtgaa agagaagagg ctggtcggcg agagggtcga gctgcggcgg cactcgcccg
     2941 taaactaccg gctatacggc gcgtggtacg ccgacccgga gatctggcgc ctgacgagct
     3001 ggtcagcggc tcccttgagc cctgccaccg tcgagcggct cttcgagggc agggaacact
     3061 ccccgacgga cgactccttc gccatccacc tcaaggacga gcgggagccc ataggcgtac
     3121 tcagcctcat gaacatcagc gaggcgaacg cctcggcgga gctctccatc atagtggggc
     3181 atccggagga caggcaccac ggctacggag ccgaggcaat cgccacgctc ctcgactacg
     3241 ccttcgggga gcgcggcctg aaccgcgtgg gcctgagcgt cttcgaattc aacgaagacg
     3301 ccatctccgc ctacgcgaag ctcgggtttc gtgaggaggg acgcctccgc cgggcgctca
     3361 agcgcgacgg cgcgttccac gacgccatcc tgatgagcgt gctcaaagac gagtgggttc
     3421 ctaggggtcc ctaggccctg atccttgcct agaactcatc ctcgagccgg gtcgcgatgc
     3481 gccggagctc gtcgttcacg gcctcgtcgg tcagggtctc ctcggcctgg aaggtgaagc
     3541 ccagcgcgac gctcttgttg ccctcgggga cctgccggcc ctcgtagacg tcgaagactc
     3601 gggtctcggc gaggatgggg ctcccgagcg actccaccgc cgcgagcatc tcgccgacgg
     3661 ggaccgagga agccaccacg accgccagat cccggctcac ggcggggacg ttgacgaaag
     3721 cttcgaagct aggctccggg tccggctcga ccggggttag atccagctcc agggccgcta
     3781 ccggccagcc gcccagctcg aacctctctg ccacctcggg gtggagctca ccgacccagc
     3841 ccacctctct gccttccaca agcaccaccg cggagcggcc gggatgcaag aacggtcgct
     3901 ccgccggctc gaagcgggcg ccgggaacaa gccgctcgac gaaacccttg gcctcgaaga
     3961 aacc
//
LOCUS       DASTUV010000246         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1274942, whole genome shotgun sequence.
ACCESSION   DASTUV010000246 DASTUV010000000
VERSION     DASTUV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274942"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFT03_04955"
     CDS             <1..556
                     /locus_tag="VFT03_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847525.1"
                     /translation="VSQSGERNVRLVARLVEHGLNTPPTSSAGRLFDAVAALAGVPGT
                     QRTTYEGQAAIELELAAHGPVNGGYPFRLRPEGDGWVVETRGIIGGVVDDLLAGREIR
                     EISSRFHRTMAEVVAAGCEEIRDAGGVSSVALSGGTFQNLLLMEQVVELLAGKGFAVY
                     RHRRVPTNDGGISLGQAVLADRGT"
     gene            567..845
                     /locus_tag="VFT03_04960"
     CDS             567..845
                     /locus_tag="VFT03_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEU4847526.1"
                     /translation="MCLGIPGRIVEILDEDLMLAKASVGGVKRNINIGLVHNEDDKVE
                     VGDWVLIHVGFAMSRLNEAEAHSTLQALEEIGEAYEQELEELKASQIE"
     gene            848..1090
                     /locus_tag="VFT03_04965"
     CDS             848..1090
                     /locus_tag="VFT03_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEU4847527.1"
                     /translation="MSEIDLTLPGSCTLDHDGCVVCSDAGIPVRVISIEGDDALCEDR
                     AGNRAEIAVELVTPVEIGEVLLTHGGVAIGKVEPDA"
     gene            1109..2236
                     /gene="hypD"
                     /locus_tag="VFT03_04970"
     CDS             1109..2236
                     /gene="hypD"
                     /locus_tag="VFT03_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEU4847528.1"
                     /translation="MKYVDEFRNAEIARAVAAEIASVTDPDRHYKLMEVCGGHTHTIY
                     RHGIKDLLPDQVELVHGPGCPVCVLPMGRVDEGITIAERPEVIFTCFGDMMRVPGSKG
                     NLLEVKARGADIRMVYSPLDALRIARENPDREVVFYAIGFETTAPSTALTLQRARKEG
                     LGNFSVFCNHVTIVPPVRALLDSPDLRLDAFIGPGHVSTVIGCRPYEFMPKDYGKPVA
                     VAGFEPLDILQSVLMLMRQLDEGRCEVENQYGRVVLWEGNLAALKSMAEVFELRPYFE
                     WRGLGFIAQSALKLSPAYADFDAELRYSTPGVRVADPKACQCGEVLKGVLKPHECRVF
                     GTACTPDHPLGALMVSSEGACAAYYNFGRKSFEKVEIGSKE"
     gene            2350..3312
                     /gene="hypE"
                     /locus_tag="VFT03_04975"
                     /pseudo
     CDS             2350..3312
                     /gene="hypE"
                     /locus_tag="VFT03_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
BASE COUNT          648 a         1123 c         1191 g          559 t
ORIGIN      
        1 agtaagccag tcgggggagc ggaacgtgcg gcttgtcgcc cggctcgttg agcacgggct
       61 gaacaccccg ccgacctcaa gcgcgggccg gctcttcgac gccgtagcag ccctggccgg
      121 tgtgcccggg acccagcgga ccacctacga gggacaggcc gccattgagc tggagctcgc
      181 cgcgcacggt cctgtgaacg gaggataccc attccggttg cggccggagg gagacggctg
      241 ggtggtggag acccggggaa tcatcggcgg ggtggtggac gacctcctgg cgggacgcga
      301 gatccgggaa atatcttcca ggttccaccg cacgatggcc gaggtagtcg ccgcggggtg
      361 cgaggagatc cgggacgccg gaggggtgag cagcgtcgcg ctctccgggg gcactttcca
      421 gaatttattg ctcatggagc aggtggtaga gttactggca gggaaaggct ttgcggtcta
      481 ccggcaccgg cgagtgccca ccaacgacgg cgggatctct ttgggacagg cggtgttagc
      541 ggacagaggc acgtgaggag gcacgaatgt gtttgggcat accgggccgg atagttgaga
      601 tcctcgacga ggatttgatg cttgccaagg cgagcgtggg cggggtcaag aggaacatta
      661 acatcgggct tgtacacaac gaggacgata aggtagaggt cggggactgg gtcctgatcc
      721 acgtgggctt cgcgatgtcc aggctcaacg aagccgaggc gcactccacc ctgcaggccc
      781 tcgaggagat aggcgaggcc tacgagcagg agctggagga gctcaaagcc tcccagatag
      841 agtgaccatg agcgagatag acctcacgct gccaggctcc tgcaccctcg accacgacgg
      901 gtgcgtcgtc tgctctgacg ccggtatccc cgtgcgggtg atctccatag aaggcgacga
      961 cgccctctgc gaggacaggg cgggcaaccg ggcagagatc gccgtggagc tggtgacccc
     1021 ggtcgagatc ggagaggtcc tgctaaccca cggcggcgtg gccatcggca aggtagaacc
     1081 tgatgcctga gcgaccggag agagcgacat gaaatacgta gacgagttca ggaacgccga
     1141 gatcgcgcgg gcggtggcgg cggagatcgc ctcggtcacc gacccggacc ggcactacaa
     1201 gctcatggag gtctgcgggg gacacaccca caccatctac cgccacggga tcaaagacct
     1261 cttgccggac caggtagagc tggtacacgg gccgggctgc cccgtctgcg tgctgcccat
     1321 gggccgcgta gacgagggca taaccatcgc cgagcggccc gaggtcatct tcacctgctt
     1381 cggcgacatg atgcgcgtgc ccggctccaa aggaaacctg ctggaggtga aagctcgagg
     1441 cgcggacatc cggatggtct actcacccct ggacgccctg cggatcgcgc gtgagaaccc
     1501 ggaccgcgag gtagtcttct acgccatagg cttcgagacg acggcgccat ccaccgcgct
     1561 aaccctgcag cgggccagga aggagggcct cgggaacttc tccgtcttct gcaaccacgt
     1621 caccatcgtg ccgccggtgc gggcgctcct cgactcgccg gatcttcgtc tcgacgcctt
     1681 catagggccg gggcacgtct cgacggtgat cggatgccgc ccctacgagt tcatgcccaa
     1741 ggactacggc aagccggtcg cggtagcagg cttcgagccg ctggacatcc tgcagtccgt
     1801 cctgatgctc atgcgccagc tcgacgaggg ccgttgcgag gtcgagaacc agtacggccg
     1861 ggtcgtcctc tgggagggga acctcgccgc cctcaagagc atggccgaag tcttcgaact
     1921 tcgtccgtac ttcgagtggc ggggcctcgg ctttatcgcc cagtccgcgc tcaagctctc
     1981 tccagcttac gcggatttcg acgccgagct gcgctactcc acgcctggcg tgcgggtggc
     2041 tgaccccaag gcctgccagt gcggcgaggt cttgaagggc gtgctcaagc cccacgagtg
     2101 cagggtcttc ggcacggcct gcacgcctga ccaccctcta ggcgctttga tggtctcctc
     2161 cgaaggcgcc tgcgccgcgt actacaactt cggccgcaag tctttcgaga aggtcgagat
     2221 aggctccaag gaatgagcga acaacgagag cgccgcgtcc tcgacgcgct cgaacagaac
     2281 cgccgccggc cgccgaagtt cacagccccg cgcatataca tgagccacgg ggcatgaggt
     2341 aaagcgaccc acaagctcat agagggcctc ctgttgccgg cgctgaacaa ccccgctctc
     2401 gcgccgctct ccgacgcggc gttgctctcg ttcggcgaaa cccaactcgc catgaccacc
     2461 gattcattcg tggtgcgtcc gctcctcttc cccggcgggt ccatcgggga gctagcggtc
     2521 aacgggaccg tcaacgacct ggcagtctcg ggagcaaccc cgatcgggct ctccgcggcc
     2581 ctcatcgtcg aggaggggct cgagaccgag gtgctcgccc gcgaggtgga ggcgattgcg
     2641 aacgccgccg tgcgggccgg ggtctccatc gccacgggag acaccaaggt agtcgagcgc
     2701 gggaaggccg acgggctcta cgtcataacc accggcgtag gagccgcgga cccggagctc
     2761 tccctctccc cagcctccgt gcggccggga gacaggatcc tctgttccgg tactctaggc
     2821 gaccatggag cggccatcct gatcgcccgc ggcgacctgg agctcgaggc agacgtgctc
     2881 tcggataccc gtccgctgac ctctctcgta gcggcgctca aagacgcagc gggaccgggg
     2941 ctccggttca tgcgagaccc cacgcgcggc ggggtgggaa cggttctcaa cgagctcgcc
     3001 cgcgactctg gatacggggt cgcgctctgg gaagacaggc tcccgatccg cgacgtcgtc
     3061 aatggggcgt gcgagatcct cggcatagac ccgctgtacg tggcgaacga aggcaagcta
     3121 ctcgccgtcg tagcacccga gctggctgac gccgcgctcg aggctttgcg cggcgtcgag
     3181 ggcggcgagg aggcccagat catcggcgag gtgcgcgagg agcccgcgcg catggtcata
     3241 atgcacacag ggttcggcgg cacccgcatg atagacatgc tcgtcggcga cccgctgccc
     3301 agaatctgtt gaggacaacc ggaggatcga agcggtgaag ttcctggtga tgtgggacct
     3361 ggctctctcc cggccgagca caggggttat acacgccgtc ctgcgcgaac aggaagagcg
     3421 atgacggtcg acacaaacgt cctcgccgtc cggagcaggg aggagttgct ccgccgtaac
     3481 gagatcttcg actccttctt caagtccgaa gccccgcgac t
//
LOCUS       DASTUV010000247         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1549558, whole genome shotgun sequence.
ACCESSION   DASTUV010000247 DASTUV010000000
VERSION     DASTUV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549558"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFT03_04980"
     CDS             <1..675
                     /locus_tag="VFT03_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEU4847529.1"
                     /translation="GASPGVGTYALPGLLERFTAAYPRVSVSVRTGHSEDILEMVLRE
                     EVQLGLTRAMRHPEVESLRLYEDEMVLVVDPGHRFTAKGVADLAEIGTEQLILFDNSS
                     SYYEQTMALFRNAGIRELRTLEVDNIEAAKRMVEHRLGVAFLPRTAIIRSVSAGNLAL
                     ISVQENPGMTRSIVALKRRDAPTSGPVDAFLEVASTLGEPQDRAELSPALATEFENLQ
                     LIDLFT"
     gene            851..1927
                     /locus_tag="VFT03_04985"
     CDS             851..1927
                     /locus_tag="VFT03_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU4847530.1"
                     /translation="MDRKTIVVMEGDQTGQELLEESLRVLDPGVTGLDLEFQRYDLSL
                     ENRRATGNQVVHEAAAAMKEAGFGIKAATVTPEGSDDVGSPNAILREEINGTVIVRTG
                     RRIPGVNPLPGIHAPISVVRMAVDDAYGAEEWREGEGVDEVAFRTERISRGVCRGVAE
                     FAFIQARKMRATVFGGPKWTVSPVYEGMLKEEMDRAAENNRDVRYDPQLIDATYALLL
                     TTHGEPLVIPTLNRDGDCLSDMVLQMFGSIAGAESLLIALDEDENPQTVMAEAPHGTA
                     PSLEGKNVANPIAMILAGAALLTFFHDEDASRASRAIYEATFEAVLDGIRTADLGGSA
                     GTTEFTDEVIRHVRTKLEVWSALA"
     gene            1952..2425
                     /locus_tag="VFT03_04990"
     CDS             1952..2425
                     /locus_tag="VFT03_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEU4847531.1"
                     /translation="MKDSNLALRAAGLGAIAGLRSMAAPAALSRAAAAGRVGGLRDTP
                     FAVLGSSKVSALLTLFEAGELIVDKLPMTPSRTSLPPLLGRAASGAVVGGALFTSERH
                     RTVPGAAFGAASAVAAAYAGERLRALASEKTGIPDPVVALAEDTVVLVGSRRLLR"
     gene            complement(2430..3305)
                     /locus_tag="VFT03_04995"
     CDS             complement(2430..3305)
                     /locus_tag="VFT03_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEU4847532.1"
                     /translation="MVEGRPSSDLLARGVEAHLGERLMSARPMGGGCIGEVYRVELED
                     GTPLVAKVDREGESHLEREAYMLRYLREKSDLPVPEVFHGSEELLLMEFVEGDSRFST
                     GAERHAAELLAALHGVTWDSYGHERDTLIGSLDQPNPPTKSWVEFFRDHRLLYLAGVA
                     HEANRLQSEDLKKIENLAERLEDYLDEPERPSLIHGDVWSANVLAKGDRITAFLDPAI
                     YYADPEIELSFISLFDSFGDAFFERYAEIRSIRPGFFEERRDLYNLYPLLVHVYFFGG
                     GYLSSVQSTLSRFSV"
BASE COUNT          632 a         1059 c         1167 g          552 t
ORIGIN      
        1 ggagcgtcgc cgggggtcgg gacctatgcg ttgcccgggc tactggagag gttcacggcg
       61 gcgtatccgc gggtctcggt ctcggtgcgg accgggcact cggaggacat cctggagatg
      121 gtgttgaggg aagaggtaca gctcgggctg acgcgggcga tgcggcatcc cgaggtagag
      181 agtttgcggc tatatgagga cgagatggtg ctggtcgtgg acccggggca ccgcttcaca
      241 gcgaaggggg tagctgacct ggcggagatc gggacggagc agctcatcct cttcgacaac
      301 agctccagct actacgaaca gacgatggcg ctgttcagga acgcggggat cagggagcta
      361 agaacgctcg aggtggacaa catcgaggcg gccaagagga tggtcgagca ccggcttggg
      421 gtggcgttcc tgcctcggac ggcgataata aggagcgtct cggcggggaa cctggccctg
      481 atctcggtcc aggagaaccc cggtatgacg cgatccatcg tcgccctcaa gaggagggat
      541 gcgccgacca gcggacccgt cgacgctttc ctggaggtag cgagcaccct gggcgagcct
      601 caggacaggg ccgagctctc ccccgccctc gccacggagt tcgagaacct ccagctcatc
      661 gacctgttta cataagacac ctgccgccgg atatgagcgt gaggcagtag gcaggcacct
      721 cgaggctgca accgaggtct tcaggcgcca cgcgcgaggg tagcgcgggc cagcctcggg
      781 ccatccgggg agcgcggggt cgtatacttg atcggaaaga acaattatct agaccagagg
      841 tgcgactaga gtggaccgca agaccatcgt agtaatggag ggggaccaga cgggccagga
      901 gctcctggag gaatctctgc gggtgcttga ccccggcgtg acgggccttg acctcgagtt
      961 tcagcgttac gacctgtcgc tggagaaccg cagggcgacc ggtaaccagg tcgtgcacga
     1021 ggcggcggcg gcgatgaagg aggcggggtt cgggatcaaa gccgccaccg tcacgccgga
     1081 gggttctgat gacgtgggat ctccgaacgc catcctcagg gaggagataa acgggaccgt
     1141 gatcgtgcgc acgggcaggc gcatccccgg cgtcaacccg ctgcccggca tccatgctcc
     1201 catctcggtg gtgaggatgg cggtggacga cgcttacggg gccgaggagt ggcgcgaggg
     1261 cgagggagta gacgaggttg cgttccggac cgagaggatc tcgcgcgggg tatgccgcgg
     1321 cgtcgccgag ttcgccttca tccaggcgcg caagatgcgg gcgacagtct tcggcgggcc
     1381 gaagtggacc gtatctccgg tctacgaggg gatgctgaaa gaggagatgg accgggccgc
     1441 agagaacaac agggacgtcc gctacgaccc ccagctaata gacgcgacct acgccctgct
     1501 cctcacgacc cacggcgagc ccctcgtcat cccgacgctc aaccgcgacg gcgactgcct
     1561 ctcggacatg gtcttgcaga tgttcggctc catagcagga gcagagtcgc tcctcatcgc
     1621 gctagacgag gacgagaacc cgcagaccgt catggcagaa gccccgcacg gcacggcacc
     1681 ctcgctcgag ggcaagaacg tcgcgaatcc catagctatg atcctggccg gagccgcgct
     1741 cctcaccttc ttccacgacg aggatgcaag ccgcgcatcg agggcgatct acgaagcgac
     1801 cttcgaggcc gtgctggatg ggatacggac cgccgacctc ggcggctcgg caggcaccac
     1861 agagttcacc gacgaggtca tccggcacgt caggacgaag ctggaagtat ggagcgcgct
     1921 cgcctagagc gaacaacaag aaggggtatg cgtgaaagat tcgaacctcg cactaagggc
     1981 tgctggtctc ggcgccatag ccgggctccg ctcgatggcc gcgcccgcgg ccctgagccg
     2041 ggctgccgcg gccggccgcg ttggaggctt gcgggacacg ccgttcgcgg tgctcggctc
     2101 ctctaaggtc tcggcgctgc tgacgttgtt cgaggcggga gagctgatcg tggacaagct
     2161 ccccatgacg ccgagccgta cctcgctacc tccactcctg ggacgggcag cctcgggcgc
     2221 cgtcgtgggc ggcgcgctgt tcacctcgga gagacaccga accgtgccgg gcgcggcctt
     2281 cggggcagcc tcggcagttg cggcggcgta tgccggggag cggctgcgcg cgctcgcgag
     2341 cgagaagacc gggatccctg accctgtcgt cgccctcgcg gaggacacgg tcgtgcttgt
     2401 gggctccagg cgactgctgc ggtaagagct cagacgctaa agcggctcag ggtactctgc
     2461 accgagctca ggtaaccgcc gccgaagaag tagacgtgca cgagcagcgg gtagaggttg
     2521 tagaggtcgc ggcgttcctc gaagaagccg ggtcttatgc tccggatctc cgcgtagcgc
     2581 tcgaaaaaag cgtcgccgaa agagtcgaag aggcttatga acgagagctc gatctcgggg
     2641 tcggcgtaat agattgccgg gtcgaggaag gcggttatgc gatcaccctt cgccagcacg
     2701 ttcgcgctcc acacgtcgcc gtggatgagg gagggccgtt cgggctcgtc gagatagtcc
     2761 tcgagccgct cagccaggtt ttctatcttc ttcagatcct cggattgcag acgattggcc
     2821 tcgtgagcta ccccagccag atagagtagc cggtgatccc ggaagaactc gacccagctc
     2881 ttggtggggg ggttcggctg gtcgaggctc cctataagcg tgtcccgctc gtggccgtac
     2941 gagtcccacg tgacgccgtg caacgccgcc agcagctcgg cggcgtgccg ctcggctcct
     3001 gtggagaagc gactatcccc ttcgacgaac tccatcaaca agagctcttc cgagccatgg
     3061 aagacttcgg ggaccggaag gtcgctcttc tcgcgcaggt agcgcagcat gtacgcctcg
     3121 cgttcgaggt gtgactcgcc ctccctgtcc accttcgcga ccagcggcgt gccgtcttcg
     3181 agctctaccc tgtagacctc accgatgcat ccgccgccca tcggcctggc gctcattagg
     3241 cgctcaccga ggtgagcttc gacgccccgc gccagcagat cggatgacgg ccgcccctcc
     3301 accactacgg gcgtccctcc ccaccatgtc cgtcggcaga gtataaggat tgccagtgac
     3361 ggtcatctac cggcatcgtg accaccgccc gcaccggcga caggtccgcg
//
LOCUS       DASTUV010000248         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_13868, whole genome shotgun sequence.
ACCESSION   DASTUV010000248 DASTUV010000000
VERSION     DASTUV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13868"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..472
                     /gene="rimP"
                     /locus_tag="VFT03_05000"
     CDS             23..472
                     /gene="rimP"
                     /locus_tag="VFT03_05000"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU4847533.1"
                     /translation="MRETARLEILAQVVEGSLPEGSELVEAKFSAGPLLTLLVDREDG
                     PVDHEFTSQVVSAVLPALEAEGYDGMLEVSSPGIERPLTRPEHFRRFVGHEAKVRLSE
                     PVEGRRNFTGVIERVGERDFALKLLEGGAEVELPFGSVSRAHLKEDI"
     gene            490..1611
                     /gene="nusA"
                     /locus_tag="VFT03_05005"
     CDS             490..1611
                     /gene="nusA"
                     /locus_tag="VFT03_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU4847534.1"
                     /translation="MSALHEIETDKGIPFETVKGVLEESLLAAYENREGAEEGARVVL
                     DRETGDLRVMKDGEDITPHDFTRIAAQVMRQTFYQRLNEVHNAQLLEEYGERMGDVVT
                     GIVQQAHRRMTIIDLGRVEAILPASEQVPSERYENGQRLKVYLLDIREPGRGPNLVVS
                     RRHEGLLKGLFHLEVPEIYDGLVEIKAVAREAGLRSKVGVWSKEPGIDPVGACVGPRG
                     SRVRAVVSELRNEKIDIIQWDPEPARFIAKALSPARVREVYLDEDEKQAEVIVPDDQL
                     SLAIGREGQNARLAVKLTDWKIDIKPESQAVEFEDEEEDWEPDEDSAMHRCRAVLSNG
                     RRCANMALPGSLFCGIPSHQQQAAEFEGMVEGSQGVTDE"
     gene            1604..>2386
                     /locus_tag="VFT03_05010"
     CDS             1604..>2386
                     /locus_tag="VFT03_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU4847535.1"
                     /translation="MSKRVYEIARELDLESKEVISRLREAGVEVKSHSSSVEDPVYER
                     VFGANGRSEAQEAEAREARAEAERSGHGATVAAAPAEEPVSAPPRRKERKRRRVVIDA
                     SATNRPTRQTAAPAVPKETEQAPPVEEKTPEEEAAAGVRVEPGATVKDLGDAIGVPPT
                     QIIKVLMGLGEMKTLTQTLSTEEIELIAVELGVDVEIGAVEEPAPAEVAPEDRPEDLV
                     EKPPVVTVMGHVDHGKTSLLDRIRSANVQSGEAGGITQHIGAY"
BASE COUNT          480 a          697 c          866 g          343 t
ORIGIN      
        1 gcttttttaa cgtcaaaaga aggtgagaga gacggcgagg ctggaaatat tggcgcaggt
       61 cgtagagggt tcgctccccg agggcagcga gctcgtggag gcgaagttct ccgctggccc
      121 cctgcttaca ctgctcgtgg accgggagga cggccccgtg gaccacgagt tcacctcgca
      181 ggtcgtctcg gctgtcttgc cggcgctcga ggccgagggc tacgatggaa tgctcgaggt
      241 atcttcgcct ggtatagagc gcccgctgac cagaccggag cacttccggc ggttcgtggg
      301 acacgaggcg aaggtccgcc tctcggagcc cgtggagggg cggcgcaact tcaccggcgt
      361 catagagcgg gtcggcgaga gagattttgc tttaaaactc ttagaggggg gagcggaggt
      421 agagctgccg ttcggctcgg tctcccgcgc gcacctcaag gaggacatat aactatgaat
      481 acggcattgt tgtcggcatt acacgagata gagacggaca agggcatccc tttcgagacg
      541 gtaaagggcg tgctcgaaga gtccctgctc gcggcatacg agaaccgcga gggggcggaa
      601 gagggggcca gggtggtcct cgaccgggag accggagacc tcagggtgat gaaggacggg
      661 gaggacataa caccccacga cttcacgcgc atagcggccc aggtgatgcg ccagaccttc
      721 taccagcgcc tcaacgaggt acacaacgca cagcttctgg aggagtacgg cgagcgtatg
      781 ggcgacgtcg ttaccggcat cgtccagcaa gcccaccgcc gcatgacgat catagacctg
      841 ggtagggtcg aggcgatcct gccagcgagc gagcaggtgc ccagcgagcg ctacgagaac
      901 ggccagcggc tcaaggtcta cttgctagat atccgcgagc caggacgcgg gccgaacctg
      961 gtggtgagcc gcaggcacga ggggctcctg aaggggctct tccacctgga ggtgccggag
     1021 atatacgacg ggctggtgga gatcaaggcc gtagcccgcg aggctgggct gcgctcgaag
     1081 gtcggggtat ggtcgaagga gccggggata gacccggttg gggcctgcgt cggcccgcgg
     1141 gggagccgcg tgcgggctgt agtctccgag ctcaggaacg agaagataga catcatccag
     1201 tgggaccccg agcccgcgcg cttcatagct aaggctctga gccctgcgcg cgttcgcgag
     1261 gtctacctgg acgaggatga gaagcaggcc gaggtgatcg tgcctgacga ccagctctcg
     1321 ctggctatcg gtcgcgaggg tcagaacgcc cgcctcgccg tcaagctgac agactggaaa
     1381 atagacataa agcccgagag ccaggcagtc gagttcgagg acgaggaaga ggactgggag
     1441 cccgacgagg actctgctat gcatcgctgc cgcgcggtcc tgtccaacgg ccggcggtgt
     1501 gcgaacatgg cgttgcccgg ctcgctattc tgcggcatcc cgtcgcacca gcagcaggcc
     1561 gcggagttcg aaggcatggt cgagggctcg cagggggtaa cggatgagta agcgtgtata
     1621 cgagatcgca agagagctgg acctggagag caaagaggtc atcagccgcc tgcgggaggc
     1681 cggggtagag gtcaagagcc actcttcgtc tgtggaagac cccgtgtacg agcgggtctt
     1741 cggtgcgaac ggccgctccg aggcacagga ggccgaggcc cgcgaggcgc gagccgaggc
     1801 ggagcgttcc ggccacggcg ctaccgtcgc ggccgcgccg gcagaggagc cggtctccgc
     1861 gccgccgaga aggaaggaga gaaagcggcg cagggtcgtc atcgacgcga gcgcgaccaa
     1921 ccgcccaacg cgccagacgg ccgctcccgc cgttcctaaa gagaccgagc aggcgccgcc
     1981 ggtcgaggag aagacaccgg aggaggaggc ggcggccggc gtgcgcgttg agccgggggc
     2041 gacggtcaag gacctgggtg acgccatcgg cgtgcctccg acccagatca taaaggtact
     2101 catgggcctc ggggagatga agacgctcac ccagaccctc tcgacagagg agatcgagct
     2161 catcgccgtg gagctcgggg tggacgtcga gatcggggcg gtggaggagc ccgctcccgc
     2221 cgaggtagcg cccgaggaca ggcctgagga cctcgtcgaa aagcccccgg tcgtcacggt
     2281 gatggggcac gtcgaccacg gcaagacgag ccttcttgac cggatccgca gcgcgaacgt
     2341 ccagtctggc gaagccggcg gcatcaccca gcacatcggc gcctac
//
LOCUS       DASTUV010000249         5661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_351451, whole genome shotgun sequence.
ACCESSION   DASTUV010000249 DASTUV010000000
VERSION     DASTUV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351451"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFT03_05015"
     CDS             <1..667
                     /locus_tag="VFT03_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4847536.1"
                     /translation="FVRAFECEAILFDLDGVLVDSTAVVVRTWREWAEDRGLDAERLL
                     EVAHGRRTAETVRLFAPDLDADSEARELERIEANDLDGVLEIEGARELLSSLPADGWT
                     VVTSGTRELASKRMEHVGLPLPERFVSADDVENGKPHPEAYLKGAEILGVRPEACAVI
                     EDAPSGVSSARSAGMRVIAVATTYGEEDLEEADAVATSLASIRATHQPESRARFELRV
                     MTG"
     gene            complement(748..1032)
                     /locus_tag="VFT03_05020"
     CDS             complement(748..1032)
                     /locus_tag="VFT03_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847537.1"
                     /translation="MSSELGVPDEKILALEEYATSPLYDEQERAALEYADRMTITGQD
                     VDDELFSRLRRFFDEDALVELTAAIAWENSSSKFNRAVRVPSQRLWKASE"
     gene            complement(1051..1293)
                     /locus_tag="VFT03_05025"
     CDS             complement(1051..1293)
                     /locus_tag="VFT03_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847538.1"
                     /translation="MARIEGVDPDRAQGQIRAVFEAQTRKWGAPLLNHLLYARRPTIF
                     RGVRAMWTGLDSSGLIDGRLVALVNRRVATINGCEF"
     gene            complement(1517..2323)
                     /locus_tag="VFT03_05030"
     CDS             complement(1517..2323)
                     /locus_tag="VFT03_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847539.1"
                     /translation="MKRILDCGPADFRQMDGAVLLEAVRRSEGRTVLCEVLWSAPPLV
                     DGVSNAEVVVAFGADLVLLNMFDGEDLAGLKDRLGRPVGVNVEPSEDVPAHRRASREN
                     LRRVADADFVVVTANPDAEVGVEEMLSAVALVRETLPGAPVFAGKMHGSGGRGMVGEG
                     EISRLAETSDAVLIAAPGTVPGSREPLVAALVDAAHASGALVVATVGTSQEGADAETV
                     RELALAAKRAGADVLHLGDAGVSGITDPMNVLAASIAIRGRRHAYRRMAM"
     gene            complement(2320..2769)
                     /locus_tag="VFT03_05035"
     CDS             complement(2320..2769)
                     /locus_tag="VFT03_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU4847540.1"
                     /translation="MEVLAPVSGRAVPLSEVPDEVFAEGMAGEGGAIVPAQSGEAVAP
                     VSGTLVKLFEGGHAFGIEADGGVELIVHVGLDTIELRGAGFEKLATEGDRVEAGQPIV
                     RFDLEEIRRGGYDPVTPVVVTNADEHPVSNLETGEVSTGDPLFEVAT"
     gene            complement(2769..4355)
                     /locus_tag="VFT03_05040"
     CDS             complement(2769..4355)
                     /locus_tag="VFT03_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU4847541.1"
                     /translation="MAQATSTPAAGGSSGWDRALGALQSVGRSLMLPIAVLPAAALLL
                     RLGQDDLLGIPWMAAAGQAIFDNLAPLFAVGIAVGLTGGAGAAGLAGLVGYFVFAGVF
                     NAVAPQLGDGETDPVFYMGVLSGIVMGLASAGLYRRFSDIRLPDYLAFFGGRRFVPII
                     TAFAALLLGIVFGLIWPPIGDAINAVGQAIVGLGALGTGLYGFINRMLIPVGLHHVIN
                     TYVWFQLGTYQGPDGPVHGEITRYFAGDPTAGYFLSGFFPIMMFGLPAACLAMIRHAR
                     VPKVASGILLSAALASFLTGITEPIEFAFLFVAPVLYLIHAILTGTALAVCTLLGIKI
                     GFGFSAGLIDYVLNFSKDNTTRPILLLGVGLVYFVIYYFVFSFFIKRFDLATPGRGED
                     STGLSADWILPESQRGPAPGDEAEREAAREAEREAESRGEREAAPAGTATAERDRDDV
                     LAENVLEALGGQENVESVEGCITRLRLFVDDPERIDEDRLKQLGASGVVKRGKVAQVV
                     MGTQSDRIAARIERLLKGEG"
     gene            4567..5514
                     /locus_tag="VFT03_05045"
     CDS             4567..5514
                     /locus_tag="VFT03_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4847542.1"
                     /translation="MLAKRRSDAAGVRDDSGAEAAAGKGGRKTSPLFEASLLLTAVFL
                     GTNPVAVKYAVGYIPPMPFVTLRFVLAGLVLWGILRLFDPKGRLRRNDLRAMAGLGLV
                     GIALNNILFTTGVSMTSASNTALVVATAPLWGMLLAFVLGWERPKPKGIIGVCLALLG
                     VGVIVYRGLGDSGASLQGDLLVMGAAACWGSYAVLSIPLLERYSPLAVASYSMLFGGL
                     AVLPLASLQLPGVDWGAVSPGGWGAIAYSALLVAAFGFTFWQRGISRIGANRMLVYQY
                     LVTLVGVIAGVVFFGESFGIDKVLGAAILLGGVYLARRQ"
BASE COUNT          939 a         1916 c         1877 g          929 t
ORIGIN      
        1 gtttgtgagg gccttcgagt gtgaggcgat cctcttcgac cttgacggcg tgctggtgga
       61 ctcaaccgcg gtcgtggtgc gtacctggcg cgagtgggcg gaggatcggg gcctcgacgc
      121 cgaacgcctc ctcgaggtcg cccacgggcg gaggactgcg gagacagtcc ggctctttgc
      181 ccctgacctg gacgccgact cggaggcgcg cgagctggag cgcatcgagg ccaacgacct
      241 cgacggagta ctcgagatag aaggagctcg cgagctgctc tcgtcgctac ctgcggacgg
      301 ctggaccgtg gtcacctcgg gcacgaggga gctcgcgtcg aagaggatgg aacacgtcgg
      361 cctgccgctg ccggagcgtt tcgtgagcgc cgacgacgtg gagaacggca agcctcatcc
      421 cgaagcctac ctgaaaggcg ccgagatcct tggcgtgcgt ccggaggcgt gcgcggttat
      481 cgaagacgca ccctcgggcg tgagctcggc caggtccgcc ggcatgcgcg tcatcgcggt
      541 cgccaccacc tacggcgaag aggatctcga ggaggccgac gcggtagcaa cctcccttgc
      601 aagcatccgc gctacccacc agcccgagtc ccgcgcgcgc ttcgagctga gagttatgac
      661 gggctagcgc ctcgtcagca cgccgccttg ggctccgcca ggcggcttgt cagcttgtgt
      721 ggcgggggct tggcgcggat actaccgtta ctctgaggct ttccacaggc gctgggacgg
      781 tacgcgcacg gcgcgattga acttgctcga ggagttctcc caggcgatgg cggcggtgag
      841 ctctaccagg gcgtcctcgt cgaagaaccg gcgcaatcgg gagaagagct cgtcgtccac
      901 gtcctgaccg gttatggtca tgcggtcggc gtactccaga gcagcgcgct cctgctcatc
      961 gtagaggggg ctcgtggcgt actcctccag cgcgagtatc ttctcgtcgg gaacgcccag
     1021 ctcgctgctc acggcagcgt tcaggtcctg tcagaactcg cagccgttta tagtcgcgac
     1081 ccgcctgttg accagggcga cgagcctgcc gtcgatgaga ccggaggagt ccagcccggt
     1141 ccacatcgcc cgcacacccc ggaagatggt cgggcgcctc gcgtacaaca gatggttcag
     1201 gagcggcgcc ccccacttcc tggtctgggc ttcgaagacg gccctgatct gtccctgcgc
     1261 ccggtccgga tccacaccct cgatcctcgc cacaacccta cctcctcgaa cacgctcccc
     1321 taaacgaaca gaagatagcc gctcacaagc cccgcaacaa cacgctgaat tgctgacaag
     1381 ctgcctgaaa ggcagcgtgc tgaaagcgaa ggcgaagccg gagcgtgctg acaagcggag
     1441 ccctaaaagg gccgaagcgt gctgacaagc cgaccgaagg gaggcgtgct gcaagcctgc
     1501 ttagcaggct tgctcatcac atcgccatgc ggcggtaggc gtgtctgcga ccccggatgg
     1561 cgatcgacgc cgccagcacg ttcattggat cggtgatccc tgagacgccc gcgtcgccaa
     1621 gatgaagcac gtccgccccg gcccgcttgg ccgcgagcgc cagctccctg acggtctccg
     1681 cgtccgctcc ctcctgcgag gtccccaccg tcgcgacaac cagcgcaccc gaggcgtgag
     1741 cggcgtcaac gagcgccgcc acgagcggct cccggctacc cgggacggta ccgggggcgg
     1801 ctatcaggac ggcgtccgag gtctcggcca ggcgtgagat ctccccctcc ccgaccatcc
     1861 cccttccgcc gctgccgtgc atcttgcccg cgaagacggg ggctccgggc agcgtctccc
     1921 tgaccagcgc gacggcggag agcatctcct cgacaccaac ctcggcgtcg gggttggcgg
     1981 tgacgacgac gaagtccgcg tccgcgacgc gccgcaggtt ctcgcggctc gctcgccggt
     2041 gcgcgggtac gtcttcggaa ggctcgacgt tcacgccgac gggccgtccg aggcggtcct
     2101 tgaggccggc caggtcctcg ccgtcgaaca tgttgaggag gacgaggtca gcaccgaagg
     2161 cgactacgac ttcggcgttc gagactccgt ccacgagtgg aggagcggac cagaggacct
     2221 cgcagaggac ggtacgtccc tcggagcgcc tgacggcctc gaggaggacc gcgccgtcca
     2281 tctggcggaa gtcagcgggg ccgcagtcga ggatgcgctt caagtggcga cctcgaagag
     2341 ggggtctccg gtgctcacct cccctgtctc gaggttgctg acggggtgct cgtcggcgtt
     2401 ggtcacgacg acgggggtga cggggtcgta gcctccgcgc cggatctcct ccaggtcgaa
     2461 gcgcacgatg ggctggcccg cctccaccct gtccccctcg gtggcgagct tctcgaagcc
     2521 agcgccgcgc agctcgatgg tgtcgaggcc gacgtggact atcagctcca ccccgccgtc
     2581 agcctctatc ccgaaggcgt gcccgccctc gaagagcttc acgagcgtcc ccgagacggg
     2641 agcgacagcc tcgccgctct gcgcgggcac gatggcccct ccctcccccg ccatcccctc
     2701 ggcgaagacc tcgtccggga cctcgctgag ggggactgcc ctgcctgaga cgggcgccag
     2761 cacctccatc aaccttcacc cttcaggaga cgctcgatgc gcgcggcgat acggtccgac
     2821 tgggtgccca tgaccacctg ggccaccttc ccgcgcttga ccacgcctga ggcgccgagc
     2881 tgcttgagcc tgtcctcgtc gatgcgctcg gggtcgtcca cgaagagcct gaggcgcgtg
     2941 atgcacccct cgacgctctc gacgttctcc tgtcctccga gcgcctcgag cacgttctcc
     3001 gccagcacgt cgtcgcggtc gcgctcggcc gtggcggtcc ctgcgggcgc ggcttcgcgc
     3061 tcgcccctgc tctcggcctc gcgttccgcc tcgcgcgcgg cttcgcgttc ggcctcgtcg
     3121 cccggcgcgg ggccgcgctg gctttcgggt aggatccaat ccgccgagag ccccgtcgag
     3181 tcctcgcccc ggccaggggt agccaggtcg aagcgcttga tgaagaagct gaagacgaag
     3241 tagtagatga cgaagtagac gaggccgacg ccgagcagca gtatgggcct ggtcgtgttg
     3301 tccttgctga agttcaggac gtaatctatg agaccggccg agaagccaaa ccctatcttg
     3361 atgccgagca gcgtgcacac cgcaagcgcc gtccccgtga ggatggcgtg gatgaggtag
     3421 aggacgggcg ccacgaagag gaaggcgaac tcgataggct cggttatgcc cgtcaggaac
     3481 gaggcgaggg ctgcggagag gaggatgccc gaggccacct tcgggaccct ggcgtgccgg
     3541 atcatcgcga ggcacgccgc ggggagtccg aacatcatga tcgggaagaa gccggaaagg
     3601 aagtagccgg cggtggggtc tccggcgaag tagcgggtga tctcaccgtg caccggaccg
     3661 tcaggcccct ggtaggtccc tagctggaac cagacgtagg tgttgatgac gtggtgcaac
     3721 cctacgggga tgagcatgcg gttgatgaac ccgtagagcc cggtcccaag tgcgccaagg
     3781 cccacgatag cctggccgac ggcgttgatg gcgtcgccta tgggaggcca gatcagaccg
     3841 aatacaatgc ccagtagcag cgcggcgaac gccgtgatta tgggcacgaa cctcctgccc
     3901 ccgaagaacg caaggtagtc aggcagccgg atgtcggaga accgcctgta caggccggct
     3961 gacgcgagcc ccatgacgat ccccgagagc acccccatgt agaacacggg gtccgtctcc
     4021 ccgtcgccga gctggggcgc cacggcgttg aagaccccgg cgaagacgaa gtacccaacg
     4081 agccctgcga gccccgcggc cccggccccg cccgtgagcc ctaccgcgat gccgaccgcg
     4141 aagagcggcg ccaggttgtc gaagatagcc tgccccgcag ccgccatcca cggtattccc
     4201 aggaggtcgt cctgcccaag cctcaacaac agcgccgctg ccggtagcac cgctatgggg
     4261 agcatcagcg agcgtcccac gctctgcaaa gccccaagag ccctgtccca cccgctggac
     4321 ccaccggccg ccggagtgct cgtcgcctga gccattacgt ccctccctct cctgctcgac
     4381 gctcgagacg cgccgatcac ccactctcta tcgttgactc taccagcgtc gtcggcgcgg
     4441 agcaaagccg ttagattatt caccagacgc ccttgcccgc tcgcccaacg atgatacgtt
     4501 ttatatatat gcgcacatca cattccccaa accagaggtc cggaaccacg gcggaagtgg
     4561 acggcgttgc tggctaagcg gaggagcgat gccgcgggcg tccgcgacga ttcgggagcg
     4621 gaggctgccg ccgggaaggg tggcaggaag acgtcgccgc tcttcgaggc ctccctgctc
     4681 ctgacagccg tcttcctggg gaccaacccc gtggcggtga agtacgcggt gggctacata
     4741 ccgccgatgc ccttcgtgac gcttcgattc gtcctggccg ggctcgtgct ctgggggata
     4801 ctgcgcctct tcgacccgaa aggcaggctc aggaggaacg acctcagggc gatggccggt
     4861 ctggggctgg tgggcatagc gctcaacaac atactcttca ccacgggcgt gagcatgacc
     4921 agcgcctcca acaccgccct cgtggtagcc acggcccctc tgtgggggat gctactggct
     4981 ttcgtgctcg gctgggaacg tccgaagccc aagggcatca tcggggtgtg cctggcattg
     5041 ctcggcgtgg gcgtgatcgt ctaccggggg ttgggggaca gcggggcgag cctgcagggg
     5101 gacctgctgg tgatgggagc tgccgcgtgc tggggctcct acgccgtgct ctcgataccc
     5161 ctgctcgagc gctactcccc tctcgccgtg gcctcctact cgatgctctt cggaggtctg
     5221 gccgtcctcc cgctcgcttc cctgcagctt ccgggcgtgg actggggggc cgtgagtccg
     5281 ggagggtggg gggccatagc ctactccgcc ttgctggtcg cagccttcgg gttcacgttc
     5341 tggcagaggg gtatcagccg catcggcgcg aaccgcatgc tcgtctacca gtacctggtc
     5401 accctggtcg gcgtcatcgc gggcgtcgtg ttcttcggag agagcttcgg gatagacaag
     5461 gtgcttggcg cggcgatcct gctcggcggt gtgtatctcg cccgccggca gtgatctcag
     5521 cacgctacgc ccttacgggc ttagcttgtc agcacgccgc cttggctacg ccaggcggct
     5581 tgtcagctag aaagcaagga ggacgcgaca tggtgcttcc ctgcgccagt ccaagcttat
     5641 gggtctctcc aagcgtgacc g
//
LOCUS       DASTUV010000250         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_82235, whole genome shotgun sequence.
ACCESSION   DASTUV010000250 DASTUV010000000
VERSION     DASTUV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82235"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFT03_05050"
     CDS             complement(<1..742)
                     /locus_tag="VFT03_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU4847543.1"
                     /translation="MRVIAGTARGTRLAPVPRGVRPTSDRVRESVFNSLGQFFEGDVL
                     DLYAGTGALGIEALSRGSERGVFVEKDRRALGTIRENLRRAKVADRAEVLAGDVGRVL
                     DRMLTDGRQFNLIFADPPYRIAATEVGGLMSRLGALLAPGGRVVIESGEAPAAGMVGK
                     KGVTRRYGGTYVTILERSEITMIVAVCPGSFDPITAGHLDIIRRASKVFDHVVVAVGA
                     NLRKESRLPAVERARLIEKVTAEMENVSV"
     gene            complement(739..>2628)
                     /gene="recG"
                     /locus_tag="VFT03_05055"
     CDS             complement(739..>2628)
                     /gene="recG"
                     /locus_tag="VFT03_05055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU4847544.1"
                     /translation="VSTRPVGRPVRGRSPGFSAQLYDGTGYLPATVWGRGWLLNQLLP
                     DTRVVVSGEVQRRYGIQIVAKSIEVIEDDPDGENGPHAGRFVPVYPVNKGIQARRMRT
                     LIHRALDAAGRVLDPLPAEVLLRHNLPNLHDAIHEVHFPTERARLTAAKRRLIFHELF
                     LIQTGLAARKARLDATEVGARHQGDGSLVNPFFKELPFGLTAAQERVVGEVLADMRSD
                     KPMRRLLQGDVGSGKTVVAVSALLTAVEAGGQGAIMAPTEVLAEQHFLSVSRSLAHLP
                     VNVALLTGSLSATERRATLDAIKSGEANIVVGTHALIQKGVEFDDLSIVVVDEQHRFG
                     VGQRTSFKEKGRTRPDTLIMTATPIPRTLSLTLYGDLEVSIIDELPPGRKPVETRLVS
                     LAERHEAYDAVRRELDTGRQAYVICPLVEESEALEDVRAAEELHEELEAEIFPDRRVG
                     LLHGRMKAADKRDVMAAFREGEIEVLVATVVVEVGVDVPNASAIVIEGSERFGLSQLH
                     QLRGRVIRGHHPPKCFLVADPRTQDAEQRLEALTRYQDGFKLSEVDLAIRGEGTLFGS
                     RQSGMPDLKVAKLLRDMDVLVEARREAFALVAGDPTLKRPDHRPLRREVKDLLGADVD
                     WLFRE"
BASE COUNT          418 a          928 c          827 g          455 t
ORIGIN      
        1 cgaccgagac gttctccatc tccgcggtga ccttctctat gagccgcgcc cgctcgaccg
       61 ccggaagacg cgactccttg cgcaggtttg ccccaactgc cacaacgacg tggtcgaata
      121 ccttcgacgc ccgccggatg atgtcgaggt gccctgccgt aatggggtcg aagctcccgg
      181 gacagaccgc cactatcatt gtgatctccg accgctcaag tatcgtcacg tacgtgccgc
      241 cgtaacggcg cgtgaccccc ttcttgccta ccatacccgc cgccggagct tcgccgctct
      301 cgatcaccac gcgacccccg ggcgcgagca gggcgccaag ccgggacatc aagcccccga
      361 cctccgtcgc cgcgattcta tacggcggat cggcgaatat caaattgaat tgtcgtccgt
      421 ctgtaagcat ccgatccagc acccgcccca cgtcacccgc caagacctca gccctatcgg
      481 cgaccttcgc ccgccgcagg ttctccctga tggtccccag ggcgcggcgg tccttctcca
      541 cgaacactcc cctctcgctc ccgcggctca gcgcctcgat gccaagagct ccggtccccg
      601 cgtacaggtc gaggacgtcg ccttcgaaga actggcccaa agagttgaag acgctctcgc
      661 gaacgcggtc cgaggtcggg cgcacgcctc tggggaccgg tgcgagccgc gtcccgcgcg
      721 ccgtaccggc gatgaccctc actcgcggaa gagccagtcc acgtccgctc cgagaaggtc
      781 cttcacctcg cgtctcaagg gcctgtgatc cggtctcttg agcgtgggat cgcctgcgac
      841 cagggcgaag gcttcacggc gggcctcgac cagaacatcc atgtcccgca atagcttcgc
      901 gaccttgagg tcgggcatcc cgctctggcg gctcccgaag agcgtgccct cgccccggat
      961 cgcgaggtcc acctccgaga gcttgaagcc gtcctggtag cgggtcagag cctcgaggcg
     1021 ttgctccgca tcctgcgtcc tcgggtcggc tacgaggaag cacttcggcg ggtgatggcc
     1081 ccggatgacg cgtccacgca gctggtgaag ctgagagagg ccaaagcgtt cggagccttc
     1141 gatcacgatg gcactcgcgt tcggcacgtc aacgccgacc tcgacgacga ccgtggcgac
     1201 gagaacctcg atctccccct cccggaaagc agccatgacg tcgcgcttgt ccgccgcctt
     1261 catcctgccg tgcaggagcc cgacgcgcct gtcggggaag atctccgcct cgagttcctc
     1321 gtggagctcc tccgctgcgc gaacgtcttc gagcgcctcc gactcttcga cgagcgggca
     1381 gatcacgtaa gcctgccggc ctgtatcgag ctcgcgccgc accgcgtcgt aggcctcgtg
     1441 acgttcggcg aggctcacga gccgggtctc gaccggcttg cgccctggcg gcagctcgtc
     1501 gatgatcgag acctcaaggt ccccgtagag cgtaagggag agcgtcctcg gtatgggggt
     1561 agccgtcatg atgagcgtat ccggcctcgt ccgccccttc tccttgaacg aggtcctctg
     1621 gcccacgccg aagcggtgct gctcgtccac caccacgatc gagaggtcgt cgaactccac
     1681 ccccttctgt atgagcgcgt gggtcccgac cacgatattc gcctccccgc tctttatggc
     1741 atcgagcgtc gcgcggcgct ctgtggcgct cagcgatccg gtgaggagtg ccacgttcac
     1801 cgggaggtgg gccagagagc gcgataccga gaggaagtgt tgttcggcga ggacctccgt
     1861 tggggccatg atggcgccct gtcccccggc ctcgacggcg gtgaggagcg cggacacggc
     1921 gacgaccgtc ttgccgctgc ccacgtcgcc ctgcaagagg cggcgcatcg gcttatctga
     1981 gcgcatgtcg gcgaggactt cgcctaccac ccgctcctgc gcggccgtga gcccgaaagg
     2041 cagctccttg aaaaaggggt tcaccaggct gccgtctccc tggtgtctgg cgcctacttc
     2101 ggtcgcatcg agacgtgctt tacgggcggc gagtccggtc tggatcagga agagctcgtg
     2161 gaagatcaga cgccgctttg cggccgtaag gcgcgccctt tcggtcggga agtggacctc
     2221 gtggatagcg tcgtgcagat tcgggaggtt gtgcctgagc agaacctcgg ccggcagcgg
     2281 gtcgaggacg cggcccgccg cgtcgagggc ccggtggatg agcgtccgca tcctcctggc
     2341 ctgtatgccc ttgttgacgg ggtatacggg cacgaaccgg ccggcgtgcg gaccgttttc
     2401 cccgtccggg tcgtcctcga tcacctcgat gctcttcgcg acgatctgaa tcccgtagcg
     2461 ccgctgaacc tctcccgaga cgacgaccct ggtatcgggc agcagctggt tcaggagcca
     2521 gccccggccc cagaccgtcg ccggcaaata cccggtgccg tcgtagagct gggcggagaa
     2581 gccgggagag cgcccgcgca ccggcctgcc caccggacgg gtgctcac
//
LOCUS       DASTUV010000251         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1585612, whole genome shotgun sequence.
ACCESSION   DASTUV010000251 DASTUV010000000
VERSION     DASTUV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585612"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1570
                     /locus_tag="VFT03_05060"
     CDS             155..1570
                     /locus_tag="VFT03_05060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847545.1"
                     /translation="MAVGVEEKSARSRDYARYSFWLETAGEELVPRPSLEGPMEADVA
                     VLGAGFTGLWTAYYLLRNEPSLRVVVLEGEVAGFGASGRNGAWCSPGFPVTPGELARR
                     YGREAARELILEMHNAVAEIGRVAEAEAIDAQYHRGGLMRVARGSSQLPGLRAAHETY
                     RALGLDGDYRMLDAEETSRRVRITGARGALYSPHCATVHPARLVRGLARAVERLGGVI
                     FERSPVTGYETGGAPRLVTARGEVRARTVVLAGEAYLTRFRELRRQLLPVYSLIVLTE
                     PLSETRWAEIGWEGRECVSSNRYTVDYLSRTADGRILFGGRGAPYHFGSRVKDRYDSH
                     GPTHEMLRRTAKEWFPALEGARFTHAWGGPLGVPRDWMPTMAYDAESGLATARGYTGQ
                     GVATANLSGRTLADLILDRDTTITRLPTVNHGSRPWEPEPLRWLGARYVQRGLMKVDD
                     RAERTGEPPIGKTLAERLARH"
     gene            1621..2748
                     /locus_tag="VFT03_05065"
     CDS             1621..2748
                     /locus_tag="VFT03_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4847546.1"
                     /translation="MEEKKTTERPQEVATEPGVRKTAVDLAGVTKSFGEFVAVDDLTL
                     EICEGEFFSLLGPSGCGKTTTLRMIAGFEEPTEGRISVAGEPVQGVPPYRRPVNTVFQ
                     SYAIFPHLNVFDNVAFGLRRTGVKGEELRNRVTEACAMVQLEGFEKRKSTALSGGQQQ
                     RVALARALVNRPKVLLLDEPLGALDLKLRKEMQLELKSLQHEVGITFVYVTHDQEEAL
                     TMSDRIAVMNEGRVQQVAEPATLYELPKNRFVANFIGQTNIFSGPVESVNGDRVTLRT
                     RAGASIEAMAPEGGVEVGDEAHATVRPEKVRFGDAGDNVVYVRIRQVVYLGVSTQYIA
                     ELADGGTLVLYQQNVHEGSGPREGDEVSVVWDTRNCRVLGG"
     gene            2751..>3599
                     /locus_tag="VFT03_05070"
     CDS             2751..>3599
                     /locus_tag="VFT03_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4847547.1"
                     /translation="MGKKISRGRFLKAMGAGAVAGSTLSVLACQPNTNPQQSGGGGGG
                     GPEEKELNFYNWADYVAKTTIPGFEKQTGIQVTQDYFSSNEELLAKLRAGGTGYDVIV
                     PSDYMVAVMIKSDVLQPLDKSKIPNFKNIGEKFRGLPYDQSNEYSVPYQWGTTGILYN
                     KKEIGQQEDSWEPVWNPEFSGKIGMLNDERETPGAALYKLGYSVNATSDEQLGEAEAE
                     LIKQKPLLRGYFDSTQNSPLVLNGDLLLGHVFSGDAFLALSQDDDLDYIIPKPAATRW
                     TDNMAIP"
BASE COUNT          700 a         1131 c         1238 g          530 t
ORIGIN      
        1 gatacagttc tcccccgcgc tcgtagccga caaagaggtt ataggcagga tcgcggaggt
       61 caccgacgcg gctctggccg agctggagcg cgagctcggc taccggagct aggatgaaaa
      121 ccgagtgcgc cagcgcatca gcaggagaca ggggttggcc gtcggcgtag aggagaagag
      181 cgctcggtcc agagactacg ctcgttacag cttctggctg gagactgcgg gcgaagagct
      241 cgtcccgagg ccgtcgctgg agggtccgat ggaagccgac gtggcggtcc tcggcgccgg
      301 tttcacgggg ctctggacgg cctattacct gctccgtaac gaaccttcgc tgcgggtggt
      361 ggtgctcgag ggcgaggtcg cgggcttcgg tgcgtcgggg cgcaacggcg cgtggtgtag
      421 tcctgggttc ccggtgacgc cgggggagct cgcgcggcgc tacggtcggg aggcggcgcg
      481 ggagctgata ctcgagatgc acaacgccgt cgcggagatc gggagggtcg ccgaggccga
      541 ggccatagac gctcagtacc accgcggcgg gctcatgcgc gtcgcccgtg gatccagcca
      601 gttgcctggc ttgcgggccg ctcacgagac ctaccgcgcg ctcggtctag acggcgacta
      661 ccggatgctg gacgccgagg agacgagcag gcgcgtccgg atcacgggcg caagaggagc
      721 gctctacagc ccgcactgcg ccaccgtgca cccggcaagg ctcgtgcgag gcctcgcccg
      781 ggccgtcgag cgcctgggtg gcgttatctt cgagcggtct cccgtcacag gctacgagac
      841 cggcggagca cctcgcctgg tgaccgcccg cggcgaggtg cgcgcgcgga cggtcgtgct
      901 cgcaggagag gcatacctga cgcggttccg cgagctgcgc cgccagctgc tacccgtgta
      961 ctccctcatc gtcctgaccg agccgctctc cgaaacccgg tgggccgaga tagggtggga
     1021 agggcgcgag tgcgtctcgt ccaaccgcta caccgtggac tacctctcgc gtaccgcaga
     1081 cgggcgcata ctgttcgggg gtcgcggcgc gccctaccac ttcggatctc gcgtaaagga
     1141 tcgatacgac agccacgggc cgacacacga gatgctgcga cgcacggcga aggagtggtt
     1201 ccccgccctc gagggcgcgc ggttcaccca cgcctgggga ggacctctgg gcgtgccccg
     1261 cgactggatg ccgacgatgg cttacgacgc tgaaagcggc ctcgccaccg cgcgcggcta
     1321 cacgggccag ggcgtggcga ccgcgaacct ctccggcagg acgctggcag acctgatcct
     1381 ggatcgtgac acgaccataa cccggctgcc aaccgtcaac cacgggtcgc gtccctggga
     1441 gcccgagccg ctgcgctggc tcggcgcgcg gtacgtgcag cgcggcctga tgaaggtaga
     1501 cgaccgcgcc gagcgcaccg gcgagccgcc aatcggcaag acgctggccg agcggctcgc
     1561 ccggcactga atacccgggc ctccgaaggt atagaatctc gaggcagaag gagtaagggg
     1621 ttggaagaga aaaaaaccac ggagcggccg caggaagtgg cgacggaacc cggagtccgg
     1681 aaaacggccg tcgatctggc cggggtaacc aagagcttcg gggagttcgt ggccgtcgat
     1741 gatctcacgc tcgagatctg cgagggcgaa ttcttctcgc tgctcggtcc gagcgggtgc
     1801 ggcaagacga ccaccttgcg catgatcgcc ggcttcgagg agcccaccga gggcaggatc
     1861 tccgtagccg gggagccggt gcagggggtt ccaccataca ggcgtcccgt caacaccgtc
     1921 ttccagtcct acgccatctt cccccacctg aacgtcttcg acaacgtagc cttcgggctc
     1981 agaaggactg gcgtcaaggg ggaggagctc aggaatcggg tcaccgaggc ctgcgcgatg
     2041 gtgcaactcg agggcttcga gaagcgcaag tcaaccgcgc tctccggcgg ccagcaacag
     2101 agggtcgcgc tggccagggc gctcgtcaac cgtccgaagg tactgctgct cgatgagccg
     2161 ttgggggctc tggatctcaa gttacgcaag gagatgcagc tcgagctcaa gagcttgcag
     2221 cacgaggttg ggatcacctt cgtctacgtc acccacgacc aggaggaggc gctcacgatg
     2281 agcgaccgga tcgcggtgat gaacgagggc agggtccagc aggttgccga acccgcaacc
     2341 ctctacgagc tcccgaagaa ccgcttcgtc gccaacttta tcggccagac caacatcttc
     2401 tccgggccgg tcgagtcggt caacggagat agggtgacgc tgcgcacccg cgcgggtgct
     2461 agcatcgagg cgatggcgcc agaaggaggc gtagaggtcg gcgacgaggc ccatgcgacg
     2521 gtcaggccgg agaaggtcag gttcggcgac gccggcgaca acgtcgtcta cgtccggatc
     2581 aggcaggtcg tctacctcgg ggtcagcacg cagtacatcg ccgagctcgc cgatggaggg
     2641 acgctcgtgc tctaccagca gaacgttcac gagggatccg gcccacgcga gggcgacgag
     2701 gtctccgtag tctgggacac ccggaactgc cgggtgctag gaggttagga gtgggaaaga
     2761 agatatcccg tggcaggttc ctcaaggcca tgggagccgg agcggtggca ggctcgacgc
     2821 tctccgtcct ggcctgccag cccaacacca acccgcaaca gagcggcgga gggggcggag
     2881 gcggtccgga ggagaaggag ctcaacttct acaactgggc ggactacgta gccaagacta
     2941 cgatccccgg cttcgagaaa cagacaggca tacaggtcac gcaggactac ttctcctcca
     3001 acgaggagct tctggccaag ctgcgggcgg gcggcaccgg ctacgacgtc atcgtgccga
     3061 gcgactacat ggtcgcggtc atgatcaaga gcgacgtcct ccagccgctc gacaagtcaa
     3121 agatccccaa cttcaagaac atcggggaga aattcagggg actcccctac gaccagagca
     3181 acgagtacag cgttccctac cagtggggga ccacgggcat cctctacaac aagaaggaga
     3241 tcggtcagca ggaggacagc tgggagcccg tgtggaaccc ggagttcagc gggaagatcg
     3301 ggatgctcaa cgacgagcgg gagaccccgg gcgcagccct ctacaagctc ggttactcgg
     3361 tgaacgcgac cagcgacgaa cagttaggag aggccgaggc cgagctaata aagcagaagc
     3421 cactcctgcg cggctacttc gactcgacgc agaacagtcc actggtcctg aatggcgacc
     3481 ttctgctcgg gcatgttttc tcaggtgacg ccttcctcgc gctgtcccag gacgacgacc
     3541 tcgactacat catccccaag ccggcggcga cccgctggac ggacaacatg gctattccg
//
LOCUS       DASTUV010000252         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_866547, whole genome shotgun sequence.
ACCESSION   DASTUV010000252 DASTUV010000000
VERSION     DASTUV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866547"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..>2166
                     /locus_tag="VFT03_05075"
     CDS             226..>2166
                     /locus_tag="VFT03_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU4847548.1"
                     /translation="MIDVNTSTVTFLFAEAGGSFSWEENRLTEMRGALSWRDRILRRA
                     VEDNGGSVYRSLDETFCAAFTSVRQALEAAVAAQRDLSAKRNGHTGEQVKMALHTGVA
                     EERDGNYFGSPVERVARLLSAARGGQVLVSAATSELVRDTSGFSELGAELRDLGEHEL
                     EASGSSERIFQLVVPELPEVAPPPIPPGSSFDERYERKELIGEGGMAAVYLAHDRELD
                     RDVAFKKLRQQYAHDEDVVERFEREAKSAASLSHSNIVSVYDRGKTGDGSYYIVMEHV
                     PGGNLKERILEEGPLPPAEAAAIALQVARALRAAHKGGVVHRDVKPQNVLLTESGEAK
                     VADFGIARAVAASTATKTGFVVGTAHYLSPEQALGHPATARSDLYSLGVVLYEMLTGE
                     LPHDAETQVGIVMKHVSGRLLRPRDVNPEVPEELDAVAARLLARDPEDRYRDADELIE
                     DLERARRGETPAFIDARHQDTSAATSPLEAREDDENGRRRYWIPLALVLFLVLLGGTA
                     YALVPDAAPEAVVPHLAGASSIEEAREMAGDRFEVVEGSRVEGTEAVGTIVAQSPYAG
                     EMAEEGSEISVDVVGTRIADVPDLQGKTREEAERILEEAGFEVEVKTTKSSAEDENLV
                     TGQNPRGDAGKTARVGSAVEITV"
BASE COUNT          425 a          649 c          744 g          348 t
ORIGIN      
        1 ggcatgttct cccttccaaa attcaccgct agtggtgcca tctcctttga agtaacgtgc
       61 gtataaagta cagtggaggc tatcgggagc cgggaatacg gtccgccata agaggggggg
      121 cgttccggcg gcccgatgtc gtgtcgctat cggctccagg ggcgttcaaa ccggacgcga
      181 ccggagcagg ctacggattg aagcgagcaa gggacgagaa agcggatgat cgacgtaaat
      241 accagcacag tcacgtttct cttcgcagag gcgggcggct cgttctcctg ggaggagaac
      301 cgtctaacgg agatgcgtgg cgccttgtcc tggcgcgacc ggatcctgcg cagggccgtc
      361 gaggacaacg gtggctccgt atacaggtcg ctcgacgaga ccttctgcgc ggccttcacc
      421 agcgtcaggc aggctctgga ggcggccgtg gcggcacagc gggacctctc cgctaaacgg
      481 aacgggcaca ctggagaaca ggtaaagatg gctctccaca cgggggtggc tgaggagcgc
      541 gacggaaact acttcggttc tccggtggag agggttgcgc gcctgctctc ggctgcacgg
      601 ggcgggcagg tacttgtctc ggccgccacc tccgagcttg tgcgcgacac ctcgggcttc
      661 tctgagctcg gggcggagct cagggacctc ggcgaacacg agctggaggc ttcggggagt
      721 tccgagagga tcttccagct cgtcgttccc gagttgccag aagtggcgcc cccgccgata
      781 cctcccgggt cgtctttcga cgagcgctac gagcgcaagg aactcatagg tgaggggggg
      841 atggccgcgg tctaccttgc ccacgaccgg gagctagacc gcgacgtggc cttcaagaag
      901 ctcaggcagc agtacgctca cgacgaagat gtagtcgagc gcttcgagcg cgaggccaag
      961 agcgccgcct cgctctccca ctcgaacata gtctccgtct atgaccgggg taaaaccggg
     1021 gacggctcct actacatcgt catggagcac gtcccgggcg gcaacctgaa ggagcgtatc
     1081 ctggaggagg gcccgctccc tcccgccgag gccgccgcga tagccttgca ggtggcgcga
     1141 gccctgaggg ccgcccacaa gggtggtgtg gtccaccgcg acgtgaagcc ccagaacgtg
     1201 ctgctcaccg agtcggggga ggcgaaggta gcggacttcg gcatcgcacg agcagtcgct
     1261 gcctcgacgg cgaccaagac cggtttcgtc gtgggcacag cccactacct ctccccagag
     1321 caggcacttg ggcatcctgc caccgcccgg agcgatctgt actccctggg tgtggtcctc
     1381 tacgagatgc taaccgggga gttgccccac gacgcagaga cccaggtagg gatagtgatg
     1441 aagcacgtca gtgggcggct tctacgcccg agagacgtga acccggaggt gcctgaggaa
     1501 ttagatgccg tagccgcacg ccttctggcc cgcgaccccg aggaccgcta ccgggacgca
     1561 gacgagctca tcgaagatct cgagcgggcg cgaaggggag agactcctgc gtttattgac
     1621 gctcgacacc aggacacgag cgcggcaact tcgccgcttg aagcgcgtga ggacgacgaa
     1681 aatgggaggc ggcgatactg gattccgttg gcgctagtac tctttctcgt gttgctcggc
     1741 ggcaccgcct acgctctcgt tccggatgcg gccccggaag ccgtggtccc tcacctcgcc
     1801 ggagccagct ccatcgaaga ggcgcgagaa atggctggcg atcggttcga ggttgtcgaa
     1861 ggcagccgcg tagagggcac agaagcagtt ggcaccatcg tggcgcagag cccgtacgca
     1921 ggtgagatgg ccgaagaggg gtcggagatc tccgtggacg tcgttggcac ccggatcgcg
     1981 gacgtgcccg acttacaagg aaaaacgcgc gaggaggccg agcgaatcct cgaggaagcg
     2041 ggtttcgagg tcgaggtcaa gaccacaaag agctccgcgg aagacgagaa cctggtaacc
     2101 gggcagaatc cgcgtggcga cgcaggcaag accgccaggg tcggctcggc cgtagagatc
     2161 acggtc
//
LOCUS       DASTUV010000253         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1108425, whole genome shotgun sequence.
ACCESSION   DASTUV010000253 DASTUV010000000
VERSION     DASTUV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108425"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFT03_05080"
     CDS             complement(<1..526)
                     /locus_tag="VFT03_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847549.1"
                     /translation="MSETITGEVKTQTIEPREKRWAVLWRRFRRNKLAVLGLVIVLVM
                     VFTAIFAPLIAPSDPKAQDYAMTLRPPGEGGLMGTDSLGSDVFSRVVYGARISLEASL
                     ISVGLAIGVGVPLGLVSGYFRGFWDEWIVMRAVDSIQAFPFLILALVIAAVLGGGLGF
                     AMIAIGIAFIPNFVR"
     gene            complement(529..1476)
                     /locus_tag="VFT03_05085"
     CDS             complement(529..1476)
                     /locus_tag="VFT03_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847550.1"
                     /translation="MAFLIRRLLLTLPILFIVSVACFSLINLIPGDPATVILGPEASE
                     HAKEQMREQLGLNKPIVVQYVDWLGGVLHGDLGKSLVDGTPVSQLILQRLPVTLELAL
                     GTFLVSLTIAVVAGILSASKRGTWVDYVSTGFALGGISIPHFWLGMMFIIIFAVYLGW
                     LPASGYVPFFEDPAANIAVMILPVLATGLRESAELMRMLRSSLLEELGSDYVRTAFSK
                     GLSRRVVVIRHAVRNALIPFVTASGLQIAALLGGLVVTEQVFQLPGVGRLIVESILER
                     DYTVVQGAVLTVTIIVILINVLVDLLYAVIDPRIALSEE"
     gene            complement(1499..3040)
                     /locus_tag="VFT03_05090"
     CDS             complement(1499..3040)
                     /locus_tag="VFT03_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4847551.1"
                     /translation="MIRGFRFGVFALFALVALVGCGGGGAPQSNTPPNTLYVALEAEP
                     PELDPNLSSAYVDRQVMASLYDKLVDIDENGEIVPMLAKSYDVSDDGKVYTFHLRDGI
                     KFHDGTEFNAEAVKYNLDRYQEEDSVRSTEVEPIESVEAVDEYTVRVTLSEPFAPFLA
                     VLTDRAGIMASPKAIEENNGRISKDPVGTGPFKFVERVRGDHITVEKNPDYWREGLPK
                     IDKIEYRGIEDENVQYQNLQSGELDIIDSIPLVEFKELQESGDYNVSIEPGLGYQGVF
                     LNVTQPPFDNKQLRQAVYRLVDRDAIVKAVLRGVGGEAGNSPFSEQSWAYSEKSDSYP
                     EPSVEEAKKLLEEAGKPDGFSFTLKTDPSPINQQIGQVIQNNLKPAGIDVKLEQEEFG
                     TLLDDSTNGNFQALFLGWSGRIDPDLNIYDFTVTNGDFNDSGYSNPEVDKLLNEARTT
                     SDRARRKELYGQVMEILHEDVPYVYLFHNNQTTDFAMQPTVQGFEPYPDGILRLAGVS
                     KEQQE"
     gene            complement(3173..3316)
                     /locus_tag="VFT03_05095"
     CDS             complement(3173..3316)
                     /locus_tag="VFT03_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU4847552.1"
                     /translation="MPSPVNPPSGCRFRTRCPYVTDACGREDPVWRDVGNDHWVLACH
                     CIP"
BASE COUNT          616 a         1061 c         1017 g          622 t
ORIGIN      
        1 tgcgcacgaa gttaggaatg aacgcgatgc cgatggcgat catcgcgaaa cccaaacccc
       61 cgcccaggac cgccgctatc acgagcgcca ggataaggaa aggaaaagcc tggatgctgt
      121 ccacggcgcg catcacgatc cactcgtccc agaaccccct gaagtacccg ctcaccaggc
      181 cgagcggcac ccctaccccg atcgcgagcc cgacggagat cagggacgcc tctagcgata
      241 tcctcgcccc gtagacgacg cgggagaaga cgtcgctgcc aagggagtcc gtgcccatga
      301 gcccgccctc gccaggcggc ctgagggtca tggcgtagtc ctgggccttg gggtcggacg
      361 gcgcgatgag gggcgcgaag atggcggtga acaccatgac caacactatc acgagcccca
      421 agacggcgag tttgttgcgc cggaagcgcc gccagaggac cgcccatcgc ttctcccgtg
      481 gctcgatggt ctgcgtctta acttcgcccg ttatcgtctc gctcacgact actcctcgct
      541 cagggcaatg cgggggtcga tcacggcgta gagcaggtcg accagcacgt ttatgagtat
      601 gactatgatg gtcaccgtga gcaccgcgcc ctgcacgacc gtatagtccc tctcgaggat
      661 cgactccacg atgagacgtc cgactcccgg aagctggaag acctgttcgg tcacgacgag
      721 cccgcccagg agagcagcga tttgcaggcc gcttgcggta acgaagggaa taagcgcgtt
      781 cctgacggcg tggcggatta cgaccacgcg ccgggataga cctttggaga acgccgtcct
      841 cacgtagtcg gagccgagct cctcgagcag gctgctgcgg agcatccgca tcagttccgc
      901 ggactccctc aaacccgtgg ccaggacggg taggatcatc accgcgatgt tcgccgccgg
      961 gtcctcgaag aaaggtacgt agccagaagc gggcagccac ccgaggtaca cggcgaagat
     1021 gatgatgaac atcatcccga gccagaagtg ggggatcgag atccccccta gcgcgaaccc
     1081 tgtgctcacg taatcaaccc aggtgcccct tttgctcgcc gacaggatgc ccgctacgac
     1141 cgctatcgtg aggctaacca ggaaggtccc caaggccagc tccagcgtca ccggcagccg
     1201 ctgcaggatc agctgagata ccggcgtccc gtcgaccagc gacttcccga ggtctccatg
     1261 cagcacgccg ccaagccagt ccacgtactg caccactatc ggcttgttca ggcccagctg
     1321 ctcgcgcatc tgctccttgg cgtgctccga agcctcaggt ccgaggatta ccgtcgccgg
     1381 atctcccggg atgaggttta tcagcgagaa gcacgccacg ctgacgatga agagtatcgg
     1441 cagcgtcagg agcagacgac ggatcaggaa cgccacgcca gggaacctcc tacctgtcct
     1501 actcctgctg ctccttgctc acgccagcga gcctcaggat gccgtccggg tagggctcga
     1561 agccctggac tgtgggctgc atggcgaagt ccgtggtctg gttgttgtgg aagaggtaga
     1621 cgtagggcac gtcctcgtgc aggatctcca tgacctggcc gtagagctcc ttgcgcctgg
     1681 ccctgtcgct ggtggtgcgc gcttcgttga ggagcttgtc cacctccggg ttgctgtagc
     1741 ccgagtcgtt gaagtcgccg ttggtcaccg tgaagtcgta gatgttcagg tcagggtcga
     1801 tgcggccgct ccagccgagg aacagggcct ggaagttgcc gttcgtggag tcatcgagca
     1861 gcgtcccgaa ctcctcctgc tccagcttga cgtcgatgcc cgcgggcttg aggttgttct
     1921 ggatgacctg cccgatctgc tggttgatcg gcgagggatc tgtcttcaga gtgaacgaga
     1981 acccgtcagg cttgcccgcc tcttcgagca atttcttggc ctcctcgacg ctgggctccg
     2041 ggtacgagtc gctcttctcg ctgtaagccc aggactgctc gctgaaaggc gagttccccg
     2101 cttcgccgcc gacgccccgg agcacggcct tgacgatggc gtcgcggtca acgagccggt
     2161 acacagcctg gcggagctgc ttgttgtcga acggaggctg cgtgacgttg aggaagaccc
     2221 cctggtagcc gagcccgggc tcgatggaga cgttgtagtc tccgctctcc tgcaactcct
     2281 tgaactcgac gagcgggatc gagtctatta tgtcgagctc tccgctctgc aggttctggt
     2341 actggacgtt ctcgtcttct attcccctgt actctatctt gtcgatcttg ggcagaccct
     2401 cccgccagta atcagggttc ttctccacgg ttatgtggtc ccccctgacc cgctcgacga
     2461 acttgaaggg ccccgtgccg accgggtcct tcgagatgcg gccattgttc tcctcaatgg
     2521 ccttcgggga ggccatgatg ccggcgcggt cggtaaggac ggcgaggaac ggggcgaacg
     2581 gctcgctcaa ggtcacccgg accgtgtact cgtctacggc ctcgaccgac tcgatcggtt
     2641 cgacctcggt gctccggacg gagtcctcct cctggtagcg gtccaggttg tacttcacgg
     2701 cctcggcgtt gaactccgtg ccgtcgtgga acttgatgcc atcccgcaaa tggaaagtgt
     2761 agacctttcc gtcgtcagag acgtcgtagc tcttggcgag catcggcacg atctcgccgt
     2821 tctcgtctat gtcgacgagc ttgtcgtaga ggctcgccat cacctgccgg tccacgtagg
     2881 cgctcgagag gttggggtca agctccggcg gctcggcctc cagagctacg tagagggtat
     2941 tcggcggcgt gttcgactgc ggcgcgcctc ccccgccaca acccaccagg gccacgagtg
     3001 cgaataatgc aaacacccca aagcggaaac ccctgatcat cgcagcgcca cccccacctt
     3061 cctgtttccc tctaccttat gaaacgcaag ctaccagtaa ctccggcaat ggtcaagtca
     3121 tcggaccggc catcgtgagg attggagata gattatgaga cgccgccggg cgttacggga
     3181 tgcagtgaca ggcgaggacc cagtgatcgt tacccacgtc gcgccacacg gggtcttccc
     3241 ggccgcaggc gtcagtcaca taggggcacc gggtacggaa acggcagccg gaaggggggt
     3301 tcacggggct tggcac
//
LOCUS       DASTUV010000254         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_4713, whole genome shotgun sequence.
ACCESSION   DASTUV010000254 DASTUV010000000
VERSION     DASTUV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4713"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFT03_05100"
     CDS             <1..764
                     /locus_tag="VFT03_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEU4847553.1"
                     /translation="LPEARETLEEFKRLSSVVYLTNKPLEMPADYAAKLTRLGVKTRP
                     EEVISSTDALLLYLARHAPDARLFVVGERPLIQLLTGAGYEVVQEAADVDVVVVSFDR
                     TFDYRKLKISFDAVRGGARIVATNPDAYCPIPGGGGLPDCAAMLAAIEASTGARAEAI
                     VGKPSPHMAATLLDRLGVPARDTLLTGDRLATDIRMANEAGMASALVLTGATGLDEAL
                     HSEDRPDYVIGHLGELLPWGDGSRAVARDPDRGNE"
     gene            768..1961
                     /locus_tag="VFT03_05105"
     CDS             768..1961
                     /locus_tag="VFT03_05105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847554.1"
                     /translation="MIPGFGAPSGGAGPEGRTPMVGGLVLPVLLGAFGVFGLFWGTFA
                     VLLADLSGALDLTPGPLGVALFAGAASSILAMALLGWTSDRLGRRLFLVVSGSLMGLG
                     IAALAFVGGYAALLGALVVLYAASGLYDVGINAAAVDLEKATERRFMALLHATFSAGG
                     VAGALSAGALLSAGVDYRHVYLALLPPLGTVVLAVAATRLPPPGGPREDATRDTGLYR
                     NLPLLLIAVIATLALLSEGEMEHWSGIYLRQTLGLSALLGGSAVAVFYGSMAVGRLGA
                     AFVIDRLGNRRTLQGAGLLAAVGMALALATREPLLVVAGFLVVGLALSAVVPIAFSVA
                     GDLAPERAGGAVSVVTTLGYGGFLIGPVIVGGLAELFSLRIALGIIGMAGALIFVLAS
                     RLKIR"
     gene            complement(2177..3898)
                     /gene="metG"
                     /locus_tag="VFT03_05110"
     CDS             complement(2177..3898)
                     /gene="metG"
                     /locus_tag="VFT03_05110"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU4847555.1"
                     /translation="MDEATRERYLVTSALPYANGPLHVGQIVGAYLPADVFVRYLRAR
                     GHDVVYVCGTDEHGVPITLTAEREGKSPREIVDYWWAHMKETFERFDISFDNFSRTST
                     ELHARNTQLFYRKLKEGGYISEEESKQMYSPTEGRFLPDRYVEGTCPVCGYKEARGDQ
                     CENCGSWYEAYELIEPHSKITGGPVEVRETTHLYLDLPKFSERLRDYVGGQEHWRGSV
                     RNFILGIIDAGLEKRPITRDISWGVPVPEPGYEDKRFYVWFDACIGYVSSTVEWAENE
                     GEPDRWREYWQEPDTRLIHFIGKDNTVFHALVWPAQLMGVGEDGEEPYVLPYDIPANE
                     FMNLEVVVDGERREVQMSTSRNLAVWLHEALDSFPADPLRFYLASILPETSDVAFSWR
                     EFQARVNNDLIGNLGNYINRTLSFTERYFDGDLPRPEEFSEEAEEVFADFRELERRYE
                     ERMLAIRPREAFAELLAMGRRANRYFDAQAPWKSRKDNPDEARGSLYAGAVILGSIAY
                     HAAPYVPEAVERLQGFFEGPIARISDLEKLPEVYRTEGAKPLFARIEDEEVAAAEEQL
                     SRAVRGE"
BASE COUNT          670 a         1403 c         1421 g          813 t
ORIGIN      
        1 tgttgccgga agcgagagag acgctcgagg agttcaagcg cctctccagc gtcgtctacc
       61 tcaccaacaa gccgctcgag atgcccgcgg actacgcggc caagctcaca cgcctcgggg
      121 tgaagacccg cccggaggag gtgatctctt cgacagacgc cttgctcctc tacctcgcgc
      181 ggcacgcgcc agatgcgcgt ctgttcgtcg tcggcgagcg gcccctgatc cagctcctca
      241 ccggcgccgg ttacgaggtg gtgcaggagg ccgcagacgt agacgtcgtg gtcgtctcgt
      301 tcgaccgcac cttcgactac cgcaagctga agatctcctt cgatgccgtg cgagggggcg
      361 cgaggatcgt ggccaccaac ccggacgcct actgcccgat ccccggcggc gggggtctcc
      421 cggactgcgc ggccatgctc gccgccatcg aagcctcgac cggggcccgt gcagaggcta
      481 ttgtcggaaa gccttcgccg cacatggcgg caacgctgct ggaccggctg ggggtcccgg
      541 ccagggacac gctgctcacc ggcgaccgcc tcgctaccga tatacgtatg gccaacgagg
      601 ccggcatggc cagcgccctg gtactgaccg gcgcgaccgg cctcgatgaa gctctccact
      661 ccgaggacag acccgactac gtcatcggcc acctcggcga actactgccc tggggagatg
      721 gatcgcgcgc agtggcgcgc gatccggata gagggaacga ataggcgatg atcccgggct
      781 tcggcgcgcc ctcgggaggg gcaggtcccg agggacgcac tccgatggtg ggagggctcg
      841 tcctgcccgt actgctgggg gctttcggcg tcttcggcct cttctgggga accttcgcgg
      901 tgctgctcgc cgacctgagc ggggcgctcg acctcacacc gggcccgctc ggggtagccc
      961 tcttcgcggg ggctgcgtcc tccatcctgg cgatggcgct tctcgggtgg acctcggacc
     1021 ggttggggcg gcgcctcttc ctcgttgttt caggctcgct catgggcctc ggtatcgccg
     1081 cgctggcctt cgtcggcggc tacgcggccc tgctcggcgc tctggtcgtg ctctacgccg
     1141 catcggggct gtacgacgtc gggatcaacg ccgccgccgt cgacctggag aaggctactg
     1201 agcgccgctt catggcgttg ctgcacgcca ccttctccgc cggcggggtg gccggtgcgc
     1261 tctctgcggg cgcgctgctc tccgccggcg tggactaccg gcacgtctac ctcgcgctgc
     1321 tgccaccgct ggggacggtg gtcctcgccg tggcggctac ccggctcccc ccgcccggtg
     1381 gaccacggga ggacgcaacg agggataccg ggctctaccg gaacctccct ctcctcctca
     1441 tagccgtgat cgccacgctg gcgctgctct cggaggggga gatggagcac tggtcaggga
     1501 tctacctgcg gcagacgctc gggctctccg cgctcctggg cggctcggcg gtcgcggtct
     1561 tctacggctc tatggccgtc ggacgcctgg gcgccgcctt cgtaatagat cgcctcggca
     1621 accggcgaac gctgcaggga gcagggctgc ttgcggccgt tgggatggcc ctcgccctgg
     1681 ctacccgcga gccgctcctc gtcgtagccg ggttcctggt ggtagggctc gccctctctg
     1741 ctgtggtgcc catagcgttc tccgtcgccg gtgacctcgc gccggagaga gcagggggcg
     1801 ccgtctccgt ggtcaccacc cttggttacg gtgggttcct gataggaccg gtgatcgtcg
     1861 gaggcctggc ggaactgttc agcctgcgga tagccctggg gatcataggg atggcgggag
     1921 ctctgatctt cgtcctcgcc tcgcgtctga aaatccggtg aacaatagta cgtatctgta
     1981 gccgtacgtg ttttctaccg tccctgtatg atcgacgttg gacacgagcg tctgcacgaa
     2041 ggatgcgact aagatccgca taaatcgcca aggaggagct ccctcacggt gcgctcccac
     2101 ctttgcgagg aggtcttcgt agtgcaggag ggcgttgctg ctgagaaggc ccgcgcgtgc
     2161 tgacaatcga cgcgctttat tccccgcgca ccgcgcggga gagttgctcc tcggccgccg
     2221 cgacctcctc gtcttcgatc ctggcaaaaa ggggcttcgc gccctccgtc cggtagactt
     2281 cggggagctt ctcgaggtcc gagatgcgcg ctataggacc ttcgaagaag ccttgcagcc
     2341 gctcgactgc ttcggggacg taaggggcgg cgtggtaggc gatggaaccc agaatgaccg
     2401 cgccggcgta aagtgatcct ctggcttcat ccgggttatc cttgcggctc ttccacggcg
     2461 cctgcgcgtc gaagtagcgg ttggcgcgcc ggcccatcgc caggagctcg gcgaacgcct
     2521 cgcgaggccg gatcgcgagc atccgctcct cgtagcgtcg ctccagctct ctgaagtccg
     2581 cgaagacttc ctcggcctcc tcggaaaact cttccgggcg aggaaggtct ccgtcgaagt
     2641 agcgctcggt gaaggagagg gtgcggttga tgtagttacc gaggttgccg atcaggtcgt
     2701 tgttcaccct cgcctggaac tcgcgccagg agaaggcgac gtcggatgtc tccggcagta
     2761 tggaggccag gtagaaccgc agagggtctg ccgggaaact gtcgagggcc tcgtgcagcc
     2821 acacggcgag gttgcggctc gtggacatct gcacctcgcg ccgctcgccg tcaaccacga
     2881 cctccaggtt catgaattcg ttggccggga tgtcgtaggg gagcacgtaa ggctcctcgc
     2941 cgtcctcgcc gacgcccatg agctgggcgg gccacaccag ggcgtggaag acggtgttgt
     3001 ccttcccgat gaagtggatg agcctggtat ccggctcctg ccagtactcc ctccacctgt
     3061 caggctcccc ttcgttctcg gcccactcga ccgtcgagga gacgtagccg atgcacgcgt
     3121 cgaaccagac gtagaagcgc ttgtcctcgt agcccggctc gggcacgggg acgccccagc
     3181 taatgtcgcg ggttataggg cgcttctcca ggccggcgtc gatgataccc aggatgaagt
     3241 tcctgaccga gccgcgccag tgctcctgac ccccgacgta gtctctgaga cgctcggaga
     3301 acttgggcag gtcgaggtag aggtgggttg tctcgcgcac ctcgacgggt ccgccggtga
     3361 tcttggagtg cggctctatg agctcgtagg cctcgtacca gcttccgcag ttttcgcact
     3421 ggtcgcccct ggcctccttg tagccgcaca cgggacacgt tccctcgacg tagcggtctg
     3481 gcaggaaacg tccctctgtc gggctgtaca tctgcttgct ctcttcctcc gagatgtagc
     3541 cgccctcctt gagcttgcgg tagaagagct gggtgttcct ggcgtgcaac tcggtggagg
     3601 tgcgcgagaa gttatcgaag gatatgtcga agcgctcgaa ggtctccttc atgtgcgccc
     3661 accagtaatc gacgatctcc cgcggcgact tgccctcgcg ctcggcggta agggtgatgg
     3721 gtacgccgtg ctcgtcggtg ccgcaaacgt agaccacgtc gtggccccgc gccctcaagt
     3781 accgcacgaa cacgtccgca ggcaggtacg ctccgactat ctgcccgaca tgcaagggac
     3841 cgtttgcgta gggcagggcc gacgtgacca gataacgctc tcttgtggct tcgtccatga
     3901 ctaaagaata gcacgctgcc ttcggcagct tgtcagctaa aagcaagaag gacgcgacat
     3961 ggtgcttccc tgcgccagtc caaggatttg cccaaagaat gtctctccaa gcgtgacagg
     4021 gcaagtgcct agcggcagtg aaagctgaaa tgctgagaag cgagggcctt ctaaaggccc
     4081 gagcgtgctg acaagctgaa atgctaatat tgcgctccgt atgttcctga aaaagctttg
     4141 ctccgttctc caggcgcgac cgcgccaccg gccgtaatgc gggtcccttt ccggcggctc
     4201 catccggagg cgtggatgcc tgccagggcg cacgcagggg acgcgggcta cgacctcagg
     4261 tcggtggatg aggcgagctt gccgcctggc gggcgggctc tgatccg
//
LOCUS       DASTUV010000255         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_609380, whole genome shotgun sequence.
ACCESSION   DASTUV010000255 DASTUV010000000
VERSION     DASTUV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609380"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VFT03_05115"
     CDS             <1..929
                     /locus_tag="VFT03_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4847556.1"
                     /translation="KPIFANPKAREILDLSAERPPKQLPDYFGDCDLGEMIARCATSR
                     ACEERWVRGDDTLFRVKFDHLPKFENHRGGVLIMIQDLSEERRLEMNQQRFLANAAHE
                     LKTPITTIIGSAELLLTGADKDAAARRRFLEHILTEGRRMQRLSDTLLRLARIGAARR
                     EPDLQSVDPEAVARRAAERIEPLAQSAEVELQFDVRRGSRVRSDPEQLEQVLLALLSN
                     AVQHSERGGSVRLGVDGVTIIVEDEGSGIDPEDLPHLFERFYRGKGSAGGFGLGLSIC
                     RDLVEGMGGRISLRPREGAGTVAEIRLPEVDA"
     gene            922..1629
                     /locus_tag="VFT03_05120"
     CDS             922..1629
                     /locus_tag="VFT03_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4847557.1"
                     /translation="MPKILIVEDDPAVRDVVDYALSREGMETEAVSSGEAATARLFSD
                     AAFDLVILDLMLPDTDGVTLCRELRSADCASRDVPVIMLTARDDETSVVVGLEVGADD
                     YVTKPFSPRQLVSRVRAHLRRQRLSSRASAMEQQKLEFPGLTIDLLRREVLAGGRPVE
                     LTAKEFEILALLASSPGRVYDREQVMAHLWGGDFFGEVRAADVHVQHIRQKIEPDPKN
                     PRYVQTVRGAGYRFAQL"
     gene            2154..2363
                     /locus_tag="VFT03_05125"
     CDS             2154..2363
                     /locus_tag="VFT03_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847558.1"
                     /translation="MIEQRHGRYFAQELVLDQNANAREQIQDALDAGEIQEWHLVGVS
                     DVLPERGVILFWDTARPSFGRSSRG"
     gene            2478..2909
                     /locus_tag="VFT03_05130"
     CDS             2478..2909
                     /locus_tag="VFT03_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEU4847559.1"
                     /translation="MPIFLYRLDLGRILGHRFLLLVHRGRESGLLRETVLEVLLYEPA
                     TKESVVLSAWGEKADWYRNVGATPALEVRSGGERYVPEQRFLEPQENHAVISDYGRRH
                     PLAFRVFARVFGYPLDGTEAARREFANSLRLVAFRPRHLES"
     gene            complement(3080..3541)
                     /locus_tag="VFT03_05135"
     CDS             complement(3080..3541)
                     /locus_tag="VFT03_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847560.1"
                     /translation="MFLSNYLGLLHQSELELAKCFRKVAEGHAAEADIYHTCNTLAKQ
                     CDAHAGKLKPFVDRYGEKREEEPERLYHELFDEIRSGSFGLLRDLHDLYVMANFCDIT
                     WTMIGQAAQGAGDVELYETVNSLEGQTSTQIKWLETRMKQAAPQTLLVASS"
     gene            complement(3544..>4607)
                     /locus_tag="VFT03_05140"
     CDS             complement(3544..>4607)
                     /locus_tag="VFT03_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU4847561.1"
                     /translation="FLVVQDIFLTETAQYADVVLPAATWGEKLGTFTNADRTVHISEK
                     AVEPPGEAKPDFEIILDYARRMDFRDKDGEPLIKWETPEECFEAWKECSRGRPCDYTG
                     ITYEKLRGGSGIQWPCNDEHPGGTERLYAEGDFNTQTDYCENYGHDLLTGATFSEADH
                     KALGPNGRAILHAAEYLPPHEEPGEEYPFRLVTGRTIYHFHTRTKTARAPQLQRAAPD
                     AWVEVHPADAGNLGIAEGDVVRVESPRGGMEAKVRICGIREGVVFAPFHYGYWDQPGG
                     HSPDGHPRAANELTITEWDPVSKQPLFKVGAVRVTKVADANGESSPTTTASAPVSAGT
                     VPATVGGEGAEVTEIVRES"
BASE COUNT          818 a         1416 c         1477 g          896 t
ORIGIN      
        1 gcaagccgat cttcgccaac ccgaaggctc gcgagattct ggacctgagt gccgagcgcc
       61 cgcccaagca gttgcctgac tacttcggcg attgcgatct aggagagatg atcgcccggt
      121 gcgccacgag ccgtgcgtgc gaggagaggt gggtgcgcgg tgacgatacc ctgtttcggg
      181 tgaagttcga ccatctaccc aagttcgaaa accaccgggg cggagtactc atcatgatcc
      241 aggacctctc cgaagaaaga cgcctggaga tgaaccagca acgcttcctg gcaaacgccg
      301 cccacgagtt gaagacgccc atcaccacca tcatcggttc cgcagagctg ctgctcacag
      361 gcgccgacaa agacgccgcg gcgaggcgcc gctttctgga gcacatcctc accgaaggga
      421 ggcggatgca acggctctcg gacaccctat tgcgtctggc gaggatcggc gccgcaaggc
      481 gggaaccgga cctgcagagc gtggacccgg aggcggtggc gcggagagcc gccgaaagga
      541 tagaaccgct tgcgcaaagc gcggaggtag agcttcaatt tgacgtgcgc cgcggctcca
      601 gagttcgttc cgatcccgag cagctagagc aggtcctcct ggccctcttg agcaacgctg
      661 tgcagcactc cgagcgcgga ggcagcgtac ggctgggggt ggacggcgtt acgatcatcg
      721 tcgaggacga agggagcggc atagatcccg aagacctccc ccacctcttc gagcgcttct
      781 accgaggcaa aggctctgca ggaggtttcg gcctcgggct gtccatctgt cgcgacctcg
      841 tcgaggggat gggaggaaga atctctctcc gtccccgcga aggagctggc acggtcgccg
      901 agatacggct gccagaggtc gatgcctaag atactcatag tcgaggacga tccggcggtg
      961 cgcgacgtgg tggattatgc cctctcgcgc gaagggatgg agacggaggc cgtctcgagc
     1021 ggcgaggcgg cgaccgcgcg cctcttctcc gacgctgctt tcgacctggt gatcctcgac
     1081 ctcatgctcc cggacaccga cggggtgacc ctctgccggg agctccggtc ggcggactgt
     1141 gcttcacgcg atgtgccggt gatcatgctc accgcccgag acgacgagac aagcgtcgtt
     1201 gtagggttgg aggttggggc cgatgactac gtcacaaagc ccttctctcc caggcaactt
     1261 gttagccgcg tacgcgcgca cctgcggcgg cagcgcctca gctctcgagc cagtgcgatg
     1321 gagcagcaaa agctcgagtt cccgggcctc actatagacc tcctgcgccg ggaggtgttg
     1381 gccggaggac ggcccgtcga gctgaccgcc aaggagtttg aaatcctcgc gctcttggcc
     1441 tcctcgccgg ggcgggtcta cgatcgcgag caggtcatgg ctcacctctg gggcggcgac
     1501 ttcttcggcg aggtccgtgc cgcagacgta cacgtccagc acatccgtca gaagatagag
     1561 ccagacccca agaacccccg ctacgtccag accgtacgtg gtgcgggtta tagattcgcg
     1621 cagttgtgag cctgcagagg cttcggggga catatcctcg cgtgatcatc aacgtttgca
     1681 ggaaactcga agtcgtggcg aaggcggcgt aaggacctcc ttccgtacac gcctgaggca
     1741 tcatggtgcc ggccgtcgaa gccagctctt ctgcgaattc ttatacgcac ctgagtgaac
     1801 aggcttgtct ttacagttac ttaaccgaag ctttacccgg ttcttaccgc ccgccatcgg
     1861 ggggtgctac tctcgtattg tgaggaggta catatgtgaa ccgtgacgaa ttattggtgg
     1921 gcttggtcgc cctcgtcgcc tgggcgcttg gtatagcggc cctggtcgtg gaatgaacgc
     1981 cgcaacggcc actggcgtca gtgacatacg ctacgttccc gaaagatgcc gtggtgttgg
     2041 atgaacaact actcacggaa ttggcactct ggtcgtgggt ggtgctcgtg ctctgggcga
     2101 cgctgccgga ttacaggtga gaacgttgcg aggcggagcg gaaggaggat catatgatcg
     2161 agcaacggca cggacggtac ttcgcccagg agttggtatt ggaccagaac gcgaatgcca
     2221 gggagcagat ccaggacgcc ctcgatgccg gcgaaatcca ggagtggcac ttggtagggg
     2281 tctctgacgt gctgcctgag cgtggcgtga tcctattctg ggacactgct aggcccagct
     2341 tcggcaggtc ctccagagga tagcctcacg acgctcaagg cggtgaggtg aaagccgctc
     2401 ctgcctctag gacccgaggc ccgaacctcc actggctcag gtcgaagccg accggagcct
     2461 cgaggcttgc gtttaggttg ccgatattcc tctaccgcct cgacttgggc cggattctcg
     2521 gacaccggtt tcttctgctc gtccaccgtg ggcgcgagag cggcctgctc cgcgagacgg
     2581 tgctagaggt actcctctac gagccggcca ccaaagagag cgtggtgctc tctgcgtggg
     2641 gggagaaggc tgactggtac cgcaacgtag gggcgacccc ggcgctggag gtaaggagcg
     2701 gaggtgaacg ttacgtgccg gagcagcggt tcttggaacc gcaagagaac cacgccgtca
     2761 tatcagacta cgggcggcgc cacccgctag ccttccgagt cttcgctagg gtgttcggtt
     2821 acccgttgga tggcaccgaa gccgcgcggc gagagttcgc aaattcctta cgcctggtgg
     2881 cctttcgccc caggcaccta gagagctaga ggacagctgg acttatagaa gactggactt
     2941 ggattcggtg gacttacccc ggttcggtag acgacgtttc cgtcacgcag cgtcttcgtc
     3001 tcccatgtac agagcgtcac cggccctgtc gtaacgttcc acgagcttcc cgtcactccg
     3061 tagtcgcggt tcttcgccat tacgaggaag ccactagaag tgtttggggg gccgcctgct
     3121 tcatccttgt ctcgagccat ttgatctgag ttgaggtctg cccttccagc gagttaaccg
     3181 tctcgtagag ttccacgtcc ccggctccct gcgcggcctg gccgatcatg gtccaggtga
     3241 tgtcgcagaa gttcgccatt acgtacaggt cgtgcaggtc gcgtaacagg ccgaagctgc
     3301 cacttctgat ctcgtcgaaa agctcatggt agagacgctc gggctcctcc tccctcttct
     3361 cgccgtagcg gtctacgaaa ggcttgagct tcccggcatg ggcgtcgcac tgcttcgcca
     3421 acgtgttaca ggtatggtag atgtcggctt cagccgcatg gccctcggcg accttcctga
     3481 aacacttggc cagttcgagc tcagactggt gcaggaggcc caggtagttc gagagaaaca
     3541 tcctcaactc tcccttacga tctcggtaac ctcggcgccc tcgccgccta cggtcgcagg
     3601 cacggtgccg gcggagacgg gcgcggaggc ggtggtcgtg ggtgaggatt caccgtttgc
     3661 gtcggcaacc ttggtgaccc tcaccgctcc caccttgaag aggggctgct tggagaccgg
     3721 gtcccactcg gttatcgtca actcgttagc cgcgcgcgga tgtccgtcgg ggctgtgtcc
     3781 cccaggctga tcccagtacc cgtagtggaa gggagcgaag acgacaccct ccctgatccc
     3841 gcatattcga accttggcct ccatacctcc gcgcggcgac tcgacgcgca cgacgtcgcc
     3901 ttccgctatg ccgaggttgc cggcatcggc cggatgcacc tcgacccacg cgtcgggtgc
     3961 cgctctctgc aactgcgggg cgcgggcggt cttggtccgg gtgtggaagt ggtagattgt
     4021 ccgcccggta acgagccgga aggggtactc ctcgcccggc tcctcgtgcg gcgggaggta
     4081 ctcggcggcg tggaggatcg ccctcccgtt tgggcccaaa gccttgtggt ccgcctctga
     4141 gaacgtcgca cccgtgagca ggtcgtggcc gtagttctca cagtaatccg tctgcgtgtt
     4201 gaagtcgccc tcggcataca gcctctcggt gccacccggg tgctcgtcgt tgcagggcca
     4261 ctgtatcccg gagccgcccc ttaacttctc gtaggtgatg ccggtgtagt cgcaggggcg
     4321 gccccgcgaa cactccttcc acgcctcgaa gcactcctcc ggcgtctccc acttgatgag
     4381 gggctcgccg tccttgtcgc ggaagtccat gcgcctcgcg tagtcgagga tgatctcgaa
     4441 gtcgggtttg gcctcgccgg ggggctcgac ggccttctcc gagatgtgga cggtgcggtc
     4501 ggcgtttgtg aacgtcccta gcttctcgcc ccacgtggcg gcgggcagaa ccacgtctgc
     4561 gtactgcgct gtctccgtga ggaagatgtc ctgcacgacc aggaaca
//
LOCUS       DASTUV010000256         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_696195, whole genome shotgun sequence.
ACCESSION   DASTUV010000256 DASTUV010000000
VERSION     DASTUV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696195"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFT03_05145"
     CDS             complement(<1..923)
                     /locus_tag="VFT03_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU4847562.1"
                     /translation="METRIPGATQNLWDPQRAVGLSELEALAYRSNLLGSDRAVANYG
                     GGNTSTKARESDHTGREIDVLWVKGSGSDLATIRAEQFTGLKLDEVLPLEEREEMSDE
                     EMVAYLAACQLSPTMPRGSIETLLHAFVPHPHVDHTHPDAINMICCSEGGEELAAECF
                     GDEAVWIPYIRPGFTLSKQVGEAVRSNPNAKIILLAKHGLVTWGETHEESYSRTIEAI
                     NRAAAFVANRAGEAFGGQEIEPLASESREALLAEVLPALRGALSSGAEEASHKILRVD
                     HTSEDVIEFVCGRASKELSQVGAACPDHLVRT"
     gene            complement(1002..2213)
                     /gene="rhaI"
                     /locus_tag="VFT03_05150"
     CDS             complement(1002..2213)
                     /gene="rhaI"
                     /locus_tag="VFT03_05150"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEU4847563.1"
                     /translation="MESTNERAYDVLAELLSNRGVDVASVEGRLRTQRVETPSWGYGN
                     SGTRFKVFPQLGVPRDPFEKLADAAEVHRLTGICPSVAIHIPWDRVDDYGELAQYAGS
                     LNMRIGAVNPNLFQEEDYRLGSVCNPNETVRRKATDHLIECVEIGRELGSKALSLWFA
                     DGTNYAGQDSFVERRHRMLECLSETYEAMPESMRMLLEYKPFEPAFYHTDLSDWGAAL
                     TMCQKLGPRAEVLVDLGHHLQGANIEHIVAILLDEGRLGGFHFNNRKYADDDLIVGSV
                     NPFELFLIYVELVGSEETTRIDYMIDQAHNIEPRIEAMILSVMNLQGAYAKALLVDRA
                     KLDGARRSGDVLLAHRLLLEAYNTDVRPLCAKVREDLGASADPISAFRESGYLEKVVD
                     ERLEGAGAGWG"
BASE COUNT          365 a          744 c          723 g          461 t
ORIGIN      
        1 gtccgtacca ggtggtctgg gcaggccgcc cctacctggg agagctcctt cgaggcgcgt
       61 ccgcagacga actcgatgac gtcctcggag gtgtggtcga cgcgcaggat cttgtgtgat
      121 gcctcctctg cccccgagga gagcgcaccg cgcagcgcgg gcaggacctc ggccagcaac
      181 gcctctctgc tctcggaggc gagcggctcg atctcctgcc ctccaaaggc ctctccggcc
      241 ctgttagcta cgaacgcggc ggcgcggttg atggcctcga tagtcctgga gtagctctcc
      301 tcgtgcgtct ctccccaggt gaccagaccg tgcttggcga gcaggatgat cttcgcgttc
      361 gggttgctac gcacggcctc gccgacctgc ttggagagcg tgaagcccgg ccgtatgtag
      421 gggatccaga ccgcctcgtc gccgaagcac tcggccgcga gctcctcgcc gccctccgag
      481 cagcagatca tgttgatagc gtcagggtgc gtgtggtcca cgtgcgggtg gggcacgaag
      541 gcgtgcagca gcgtctctat ggagccgcgg ggcatggtgg gcgagagctg gcaggcagcg
      601 aggtaggcga ccatctcctc gtcgctcatc tcctcgcgct cctcgagcgg caggacttcg
      661 tcgagcttga ggccggtgaa ctgctcggcc cgtatggtcg cgaggtcgct ccctgagccc
      721 ttgacccaga gaacgtctat ctctctcccc gtgtggtcgc tctccctggc tttggtcgag
      781 gtgttacccc ctccgtagtt ggccaccgcg cggtcgctgc ccagaaggtt ggagcggtag
      841 gcaagggcct ccaactcgga gagacccacg gctctctgcg ggtcccagag gttctgggtg
      901 gctcccggta tcctagtctc catcgcttct ccgaaccttc ctttcccggt cagctctttc
      961 gtgcaacgtc cggccttcgc cttcctggct ttcgcctcgt tctatcccca gccagctcct
     1021 gctccctcaa gtcgctcgtc taccaccttc tcgaggtagc cgctctccct gaaagcggag
     1081 atgggatccg ccgaagcgcc gaggtcttcc ctgaccttcg cgcacagggg ccgcacgtcg
     1141 gtattgtagg cttcgagcag aagccggtgc gccagaagca cgtcgccgga gcggcgcgcc
     1201 ccgtcgagct tcgcgcggtc cacaaggagg gccttcgcgt aggcgccttg caggttcatg
     1261 acggaaagga tcatggcctc gatcctgggc tcgatgttgt gggcctggtc gatcatgtag
     1321 tcgatgcgcg tcgtctcctc agacccgacg agctcgacgt agatcaggaa caactcgaag
     1381 gggtttacag atcccacgat gaggtcgtcg tcggcgtact tgcggttgtt gaagtgaaag
     1441 ccacccagcc gcccctcgtc gagcaagatc gcaacgatgt gttctatgtt ggcgccctgg
     1501 aggtggtggc cgaggtctac cagaacctcc gctctcggcc cgagcttctg gcacatggtg
     1561 agcgccgcgc cccagtcgga gagatccgta tggtagaaag caggttcgaa aggcttgtac
     1621 tccagcagca tgcgcatact ttcgggcatg gcctcgtagg tctcggagag gcactcgagc
     1681 atgcgatgac gccgctctac gaaggagtcc tggccagcgt agttggtgcc gtcggcgaac
     1741 cagagggaga gggccttcga gcccaactcg cgtcctatct cgacgcactc gatgaggtgg
     1801 tcggtagcct tgcggcgcac ggtttcgtta gggttgcaga cgcttccgag cctgtagtct
     1861 tcctcctgga agagattcgg gtttacggcc ccgatcctca tattcagcga gcccgcgtac
     1921 tgcgctagct ccccgtagtc atcgacccta tcccagggga tgtggatagc gacggaaggg
     1981 cagatgcctg tgagccggtg gacctcggcg gcatccgcca gcttctcgaa ggggtcgcgg
     2041 gggacgccaa gctgcgggaa gaccttgaag cgggtccccg agttgccgta gccccacgac
     2101 ggggtctcta ctctctgggt acgcagccga ccttccacgc tcgccacgtc cacaccacga
     2161 ttcgatagaa gctcggccag tacgtcgtag gcccgctcgt tcgtgctctc caagatccta
     2221 ccttccttct gaccgctaca tctcgaagca ctggcagctc ggacgacgcg ccctcatcgg
     2281 tgactgtgct cgt
//
LOCUS       DASTUV010000257         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1507248, whole genome shotgun sequence.
ACCESSION   DASTUV010000257 DASTUV010000000
VERSION     DASTUV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507248"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFT03_05155"
     CDS             complement(<1..461)
                     /locus_tag="VFT03_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4847564.1"
                     /translation="MSGKERPDPARRARITWLGHSTVLVELDGTRLLTDPVLRDRVAH
                     LRRTKPADAGMLYALDGVLVSHLHYDHLDYPSLERLGRSVPLVVPRGAGGLLRRRRFE
                     RVIEVDAGEEVRIGDLAVRATPADHEGGRGFLGTEVAALGYLIRGSHRIYFA"
     gene            complement(474..2537)
                     /locus_tag="VFT03_05160"
     CDS             complement(474..2537)
                     /locus_tag="VFT03_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU4847565.1"
                     /translation="MGLADRHAVLRVFVVWVVTAVTLFVLSALIPEIEIPGWGSAFAI
                     AAVIGLLNAVIWPLLVRFALPITVLTLGVAALLLNGVIVGIASRIVDGFGVGMLWGIV
                     VALVLTVVNTLVTSLLGIDDDDFYYRNVIRRAATRQGATSSEVPGVVFLEIDGLAHEV
                     VVRALRDGNMPLVARWLREGSHQLIRWETDWSSQTGACQAGLLHGNSDDWPAFRWWEK
                     EHNRPIVTNHPKDAAELEHRHSNGRGLLHEDGASRANILSGDAKHSLLTMSTVLRRDR
                     PGRIGQDYFAYFANPYNITRTVVFALADIAQELYYAAQQRRRDIRPRIKRTLPYALVR
                     SWATVIQRDLQVSAIIGDLYAGRPVAYTTFLAYDEVAHHSGIERPDTLSVLRKIDRQF
                     ARIEQAAKEAPRPYHLVVLSDHGQSQGSTFLERYGASLEDIVREACATDSIHVDEGGS
                     NEASGYLGASLTEAGSADNAVGRTIRVATKGKRVDGAVQLGEKSREETVRMREAAQDG
                     QLPELSVMASGCLGLISFPREPGRLTLERIERLYPELVPALRDHPGIGFMLVRSERHG
                     AVAIGAHGTHYLDEDRVEGKDPLAPFGPNAARHVRRADGFEHAADVMVNSTYFADTDE
                     VAAFEELVGSHGGMGGSQSFPFVVMPADWRLPEQSIVGPDHMHRHMRRWLADLGHERY
                     RDGAT"
     gene            2888..3403
                     /locus_tag="VFT03_05165"
     CDS             2888..3403
                     /locus_tag="VFT03_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847566.1"
                     /translation="MGPVQLVAIGFPPGANFEGRIMDELSRLEQHETIRILDLLFVGK
                     DEETGDLLALDYQGEDLGAIVGALLGFEFEDNGERQAESAQGLQSHAFGLSQREIEEI
                     AASLAPGSSAGFLLVEHVWARDLKRAIRDAGGFPLGEGFLTPEAIAAVAVELVAMSEA
                     LNELEMEEAGA"
     gene            3454..3747
                     /locus_tag="VFT03_05170"
     CDS             3454..3747
                     /locus_tag="VFT03_05170"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU4847567.1"
                     /translation="MGLMAERAAGRMQARQTYRTMARMERRRSFVQSKVGMRQDFGRE
                     DQPAEATEAAPAPAATSSEPGYVGELERLAQLRDQGILTEEEFEAKKKQLLGI"
BASE COUNT          767 a         1282 c         1338 g          695 t
ORIGIN      
        1 gcgaagtaga tccggtgcga gccgcggatc aggtaaccca gggctgccac ctccgttccc
       61 agaaatcctc gtcctccttc gtggtcggcg ggggttgcgc gcacggcgag atccccgatc
      121 cgaacctcct cgccggcatc gacctcgatg acccgttcga agcgacgccg gcggagcagc
      181 ccgccggcgc ctcgcgggac gacgagaggt acagatcgac cgaggcgctc gagtgaggga
      241 tagtcaaggt gatcgtagtg gaggtgggag acgaggactc cgtcgagagc gtagagcatc
      301 ccagcgtctg ccggttttgt acgccggagg tgcgccaccc ggtcgcggag caccgggtcg
      361 gtcaggagtc gcgtgccgtc taactcgacc aggaccgtgc tgtggccgag ccaggttatg
      421 cgcgcccggc gcgctgggtc gggacgctcc ttaccactca ccgcgaccac tgctcaggtg
      481 gcgccgtccc ggtaccgctc gtgaccgagg tcggcgagcc aacggcgcat gtgccggtgc
      541 atatggtcgg ggccgacgat ggattgctcg ggcagacgcc agtccgcggg catgacgacg
      601 aacgggaacg actgggaacc gcccatgccg ccgtgtgatc ccacgagctc ctcgaaggcg
      661 gcgacctcgt ccgtatccgc gaagtaggtg ctgttaacca tgacgtcggc ggcgtgctcg
      721 aagccgtccg cgcgcctgac gtgccgggcg gcgttcgggc cgaagggtgc caaaggatcc
      781 ttcccttcca cccggtcttc gtccaggtaa tgcgttccgt gagcacctat cgccaccgcc
      841 ccatgccgct cggagcgcac cagcatgaag ccgatgccgg ggtggtcgcg cagcgccggg
      901 acgagctctg ggtagagacg ctcgatgcgc tcgagcgtaa gccggcctgg ctcgcgcgga
      961 aacgagatga gccccaggca gcccgaggcc atcaccgaca gttcgggcaa ttggccgtcc
     1021 tgcgccgctt cgcgcatcct tacggtctct tcacgagatt tctcgccgag ttgcacagca
     1081 ccgtctaccc gcttgccctt cgtcgccacg cggatcgtgc ggccgactgc gttgtctgct
     1141 gacccagctt cggtcagcga cgcgccaagg tatcctgacg cctcgttgct tcccccttcg
     1201 tccacgtgga tgctgtcggt ggcgcacgcc tcgcgcacga tatcctccag cgacgcgccg
     1261 tagcgctcga ggaaggtgga gccctgcgac tgaccgtggt cggagaggac cacgagatgg
     1321 tacgggcgtg gggcctcctt cgccgcctgc tcgatccgcg cgaactgccg atcgatcttg
     1381 cgcagcaccg acagagtatc cggtcgctcg atgccagagt ggtgggcgac ctcgtcgtag
     1441 gcaagaaagg tcgtgtaggc gaccggccgg cctgcgtaga ggtcgcctat gatcgccgac
     1501 acctggaggt cgcgctggat caccgtagcc cacgagcgca cgagcgcgta gggtagggtg
     1561 cgcttgatcc gtgggcggat gtcgcgccgg cgctgctggg cagcgtagta gagctcctga
     1621 gcaatatcgg ccaaggcgaa cacgactgtt ctggtgatgt tgtaagggtt ggcgaagtaa
     1681 gcgaagtagt cctgaccgat gcgcccgggt cgatcgcgcc tcagcaccgt gctcatcgta
     1741 agcaggctgt gtttcgcgtc gcccgagagg atgttcgccc ggctggcacc gtcttcgtgc
     1801 aacaggccgc gtccgttcga atgacgatgc tccaactcgg cggcgtcctt gggatggttc
     1861 gtgacgatcg gtcggttatg ctccttctcc caccagcgaa acgccggcca gtcgtcgctg
     1921 ttgccgtgca gcagtccggc ctggcaagcg cccgtctgcg acgaccagtc cgtctcccac
     1981 cggatgagct ggtgcgatcc ttcgcgcagc cagcgggcaa ccagcggcat gttgccgtca
     2041 cgtagcgcgc gcacaacgac ctcgtgcgca aggccgtcga tctcgaggaa tacaacgcca
     2101 ggtacctcgg acgaagtagc tccttgacgc gtggcggctc gccggatgac gttgcggtag
     2161 tagaagtcgt cgtcgtcgat tccaaggaga gaggtgacga gcgtgttcac taccgtgagg
     2221 accagggcta cgacgatccc ccagagcatc ccgactccga aaccatctac gatgcgcgat
     2281 gctatcccca cgatgacgcc gttcaggagt agcgcggcga ctcccaaggt caacacggtg
     2341 ataggcagcg cgaacctgac caacagtggc cagatcacgg cgttgaggag accgataacg
     2401 gccgcgatag cgaaggcaga gccccagccc gggatctcta tctcagggat gagagcggag
     2461 aggacgaaga gcgttacagc agtgaccacc cacacgacaa acacccggag cacagcgtgg
     2521 cggtcggcca agcccatcac gagtatcctc gactgggcgc cgtccggtcc gaagcgcaga
     2581 taccgttagt gcgttcctct gcaatatcga gcaagggctt cctccggtca ccagggcgca
     2641 tccacagcag agaccgccct tcggggtagc gatccatcgt gccatctttt cgagccgctt
     2701 gccgctactc tgcatgacgc ttccccaaca tacgccccga tttggcgatg agtcatccca
     2761 cgcaggcgaa tttcccctgg gggatatgcg tccctgggtg aagacgccgc actgaccgcc
     2821 acgtacgatg tggccccagg atggagaacc gcgattacaa cgacggaggt ggatgatgag
     2881 tggagagatg ggaccggttc aactcgtagc gattggcttt ccccctgggg cgaacttcga
     2941 gggcaggatc atggacgagc tctcaaggct cgaacaacac gagactatcc gcatcctcga
     3001 cttactcttt gtgggcaaag acgaagagac tggagacctt ctcgccctcg attaccaggg
     3061 cgaggatctg ggtgcgatcg tgggcgctct gctcggcttc gagttcgagg acaatggtga
     3121 gcggcaagcc gaatctgcgc aggggcttca gagccacgcc ttcgggctct cgcagaggga
     3181 aattgaggag atagcggcct cgctcgctcc cggcagctct gcaggcttcc tgctcgtcga
     3241 gcacgtgtgg gctcgcgatc tgaagcgcgc gatccgcgat gcgggcggtt ttccgctcgg
     3301 agagggcttc ctcacccccg aggcaatcgc cgccgtagcc gtcgaactcg tcgccatgtc
     3361 agaggccctc aacgagctcg agatggagga ggcgggtgcc taggcaagct gataagggtt
     3421 gaacgaacgt agagaagaaa ggagatcgca gagatgggac tcatggcgga acgggcggca
     3481 ggcaggatgc aggcgcggca gacctaccgg accatggcgc gcatggagcg acgccgctcg
     3541 ttcgtccaga gcaaggtggg tatgaggcag gacttcggcc gtgaggatca gcccgcggag
     3601 gccacggagg ctgctcctgc tcccgccgcc acgtcatccg agcccgggta cgtgggcgag
     3661 ctggagcgcc tcgcgcagct tcgtgaccag ggcatcctca ccgaagagga gttcgaggcc
     3721 aagaagaaac agctacttgg gatctgagag ccaagcgggc gcgaggctag tgcagacccg
     3781 atgcagtcca accccggtaa gggcggtagc ttcatcggct cccgggagtg cctcgaggac
     3841 gcgatgagga tcagcagaga atttctgcgt gcgtttcggc tcaagacaac tgctggagga
     3901 gaaagagagc caaaggagcc agagtagtaa tgagcctagc gatcctcccc ctcgccatca
     3961 ccatgaacgc agggccacag atcatgtcgg ccctcatctt catcaccgcc tcaaagcccc
     4021 tcaagctctc tgccgccttc ctcgctggag ttgtcattgc cgtaagcgtg ggcctcacca
     4081 tc
//
LOCUS       DASTUV010000258         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1109965, whole genome shotgun sequence.
ACCESSION   DASTUV010000258 DASTUV010000000
VERSION     DASTUV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109965"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..2010
                     /locus_tag="VFT03_05175"
     CDS             229..2010
                     /locus_tag="VFT03_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU4847568.1"
                     /translation="MAYLPIEDHGIIGDLYTAALVGTDGTIDWLCLPDFDSPSVFCSI
                     LDDEKGGYFKIRPVQDFHSYQIYLPETNVLLTRFLSREGVAEVLDFMPIEDASETRHD
                     LVRDVRVIRGRMTVEVECRPAFDYARREHEIEPARGGVIFRSAGTALNLACEVPLEEG
                     PGRGVRGRFELKTGESASFVLQLLEEGEGPREVLKRAECQDLLQQTMDYWRRWIAGSA
                     YRGRWRDMVNRSTLALKLMTYEPTGALVTAPTMGLPQTIGGAQNRDYRYTWLRDAAFT
                     MFVLLRIGFKDEAHRFMGWLQQRCQGSGGLLNPLYGLDGRKEVVEEELSHLSGYRGSR
                     PVTLGNGANRLLQFDLYGAVLDAAYLHNKYGAPLDYDTWQSLRTLLSWLSENWRRPDA
                     GTWGDRDHLDQFVSSKVFSWVALDRAIRLAYQRGLPGEEELWIGQRDAIYQEVMQKGW
                     NAEKGSFVEHYGSGTLDASLLLMPVTQFVGPTDPRWLATLDRIQNELAHDTLVDRYEA
                     ADADGSATEEGTFNVCSFWLVECLTRAGRLDEARLALEKMFSYANHLGLYAEKLGSSG
                     EALGNFPHAFTHLSLITASVRLDRALS"
BASE COUNT          402 a          712 c          733 g          396 t
ORIGIN      
        1 gatagcttcc tggactcgct gctggatgcc ggtcacggtc gctccccttt cgacttcttc
       61 atgctcatgt aatctcgagg cagcacttgg ggctgggcgg gcttctgact cgaccacggc
      121 gccggcgtca tccgggcaag ctcctcccgt acccatcggg gtctttgccg tgcgcggtcc
      181 gtgggggcac aatgtaggag gtactgtcgg cactggtggg gaagacgaat ggcgtacctc
      241 cctatagagg accacgggat cataggggac ctgtacaccg ccgccctggt gggcacagac
      301 gggaccatag actggctgtg tctgccggac ttcgactccc cgagcgtctt ctgttccata
      361 ctcgacgacg agaagggcgg ttacttcaag atccgcccgg tccaggactt ccacagctac
      421 cagatctacc ttccagagac caacgtgctt ctcacccgct tcctctcccg ggaaggcgtg
      481 gcggaggtac tggacttcat gcccatcgag gatgcgagcg agacgcgcca cgacctcgtc
      541 cgcgacgtgc gggtcatcag ggggaggatg accgtcgagg tagagtgccg cccggccttc
      601 gactacgccc gccgggagca cgagatcgaa cccgccaggg gaggtgtgat cttcaggagc
      661 gccggcacgg ccttgaacct ggcctgcgag gtgccgctcg aagaagggcc gggccggggc
      721 gttcgcggac ggttcgagct gaagactggc gagagcgcca gtttcgtcct gcaactgctc
      781 gaagaaggcg aggggccaag ggaggtcctg aagcgggcgg agtgccagga tctgctgcag
      841 caaacgatgg actactggcg gcggtggatc gcggggagcg cctaccgcgg gcggtggcgg
      901 gatatggtga accgctccac tctcgctctc aagctgatga cctacgagcc gaccggggca
      961 ctggttaccg cgccaactat ggggctgccg cagaccatag ggggagcaca gaaccgggac
     1021 taccgctaca cctggttgcg cgacgcggcc ttcactatgt tcgtcctcct caggatcggc
     1081 ttcaaagacg aggcgcacag gttcatgggc tggctccagc agcgctgcca gggctcgggc
     1141 ggccttctca atccgctcta cggcctggac ggtaggaaag aggtcgtgga agaagagctc
     1201 tcccacctct ccgggtaccg tggctcccgt cccgtgacgc tgggaaacgg cgcgaacagg
     1261 ttgctgcagt tcgacctcta cggtgccgtt ctggacgcgg cctacctgca caacaagtac
     1321 ggcgcgcccc tcgactacga tacctggcag agcctgcgca ccctcctgag ctggctgagc
     1381 gagaactggc ggcggccgga cgccggaacg tggggggatc gggatcacct cgaccagttc
     1441 gtatcgtcga aggtgttcag ctgggtggcg ctcgaccggg cgatacgact ggcctaccag
     1501 cggggactcc ccggcgagga ggagctctgg atcggtcagc gagacgccat ctaccaggag
     1561 gtcatgcaga agggttggaa cgcggagaag gggagcttcg tcgagcacta cggctccggc
     1621 accctggacg cctcgctgct gctcatgccc gtgacgcagt tcgtcgggcc gacggacccc
     1681 cgctggctcg ccaccctcga tcgcatacag aacgagctgg cccacgacac gctggtggac
     1741 cgctacgagg cggcggacgc cgatggctca gcgaccgaag aggggacctt caacgtctgc
     1801 tcgttctggc tggtggagtg cctcaccagg gccgggcgcc tggatgaggc aaggctcgct
     1861 ctggagaaga tgttctccta cgccaaccac ctggggctgt acgcagaaaa gctaggatct
     1921 tccggagagg ctcttggcaa cttcccgcac gccttcaccc acctttccct gatcaccgcc
     1981 tccgtgcgcc tggatcgggc cctgagctag agatctccgt ccagctagaa ggcgagcggc
     2041 ctggtctctt gcccccgcgc tcttgcaaca ggtgcgagcc taccgccggc actgtgtcgg
     2101 tccactgctc cgggaggttt gacacttttc caaaggcacc caatcagcga gacttgcatc
     2161 gagctagcgg cctgcttcca ttggtgtcat tggttgcccc aaattccgcc cactactcgc
     2221 taaggaagcg ctcaaggaac tcg
//
LOCUS       DASTUV010000259         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1467211, whole genome shotgun sequence.
ACCESSION   DASTUV010000259 DASTUV010000000
VERSION     DASTUV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467211"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /gene="rplF"
                     /locus_tag="VFT03_05180"
     CDS             complement(<1..335)
                     /gene="rplF"
                     /locus_tag="VFT03_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU4847569.1"
                     /translation="MSRIGKAPVEIPGTVSVELSGRSVKVNGPKGELTVPVGHGIDVR
                     QEDGNVIVERAGDTPEHRAMHGLTRSLIYNAVTGVTEGFTKTLNIAGVGYRAALQGSD
                     INLQVGYSHP"
     gene            complement(350..748)
                     /gene="rpsH"
                     /locus_tag="VFT03_05185"
     CDS             complement(350..748)
                     /gene="rpsH"
                     /locus_tag="VFT03_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4847570.1"
                     /translation="MTVTDPIADFLTRIRNAHMVKQETVEIPHSKMKAEISRILLEEG
                     YVQDVSERGSGVEKRLVIALKYGPDGSRGIAGLRRMSRPGRRVYRKQTNIPRVLDGLG
                     VAILSTSQGILTDHQARRRGVGGEVLCFVY"
     gene            complement(752..937)
                     /locus_tag="VFT03_05190"
     CDS             complement(752..937)
                     /locus_tag="VFT03_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU4847571.1"
                     /translation="MTRKALLVKQQKKQKYAVREYNRCQRCGRAHGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            complement(948..1490)
                     /gene="rplE"
                     /locus_tag="VFT03_05195"
     CDS             complement(948..1490)
                     /gene="rplE"
                     /locus_tag="VFT03_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4847572.1"
                     /translation="MARLKERYHEEVVPDLEERFGIENPMRMPGLEKIVVNMGVGEAV
                     VNSRALDGAMEDLARITGQKAQLRRARKSIAGFKIREGMPVGARVTLRGERMWEFLDR
                     FISIALPRVRDFRGVSPASFDGRGNYAIGLREQVIFPEISYDSIDATRGLDVAIVTTT
                     ESDEEARELLRLLGMPFRQS"
     gene            complement(1483..1794)
                     /gene="rplX"
                     /locus_tag="VFT03_05200"
     CDS             complement(1483..1794)
                     /gene="rplX"
                     /locus_tag="VFT03_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU4847573.1"
                     /translation="MKLKRGDTVVVISGKEKGKRGEIERVIPKENRVVVGGVNVRTRH
                     ARPSQQNQQGLYHFEAPIDVSNVMLVDPNSGEPTRVGYRFTDSGEKLRISKKSGKDID
                     G"
     gene            complement(1803..2171)
                     /gene="rplN"
                     /locus_tag="VFT03_05205"
     CDS             complement(1803..2171)
                     /gene="rplN"
                     /locus_tag="VFT03_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU4847574.1"
                     /translation="MIQQESRLRVADNSGARSILTIRVLGGSKRRSAGVGDVIVATVK
                     EATPGSAVKKGDVVRAVVVRTKKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELREKGYTRIISLAPEVL"
     gene            complement(2171..2437)
                     /gene="rpsQ"
                     /locus_tag="VFT03_05210"
     CDS             complement(2171..2437)
                     /gene="rpsQ"
                     /locus_tag="VFT03_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU4847575.1"
                     /translation="MQTDTERNRRKERVGLIVSDRADKTVTVSVEALVRHPMYKKRVR
                     RSRKFMVHDERNEARVGDTVRIVETRPLSARKRWRLANIISRAE"
     gene            complement(2585..2776)
                     /gene="rpmC"
                     /locus_tag="VFT03_05215"
     CDS             complement(2585..2776)
                     /gene="rpmC"
                     /locus_tag="VFT03_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU4847576.1"
                     /translation="MKAAEVRELDVEELEQRLAATRRELFNLRFQHATGQLENTGQLK
                     EVRKNIARILTVLNQKAGE"
     gene            complement(2773..3192)
                     /gene="rplP"
                     /locus_tag="VFT03_05220"
     CDS             complement(2773..3192)
                     /gene="rplP"
                     /locus_tag="VFT03_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4847577.1"
                     /translation="MLVPKKLKYRKPHRGRMRGKAKGGTDVQFGEYGLQALEPAWITN
                     RQIEAARIAMTRKIRRGGKVWINVFPHKPVTKKPAETRMGSGKGSPEAYVAVVKPGRV
                     MFEMSGVDETLAREAMHLAAQKLPIKTRFLTRGGGQQ"
     gene            complement(3196..3864)
                     /gene="rpsC"
                     /locus_tag="VFT03_05225"
     CDS             complement(3196..3864)
                     /gene="rpsC"
                     /locus_tag="VFT03_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU4847578.1"
                     /translation="MGQKVHPEGFRLGVQRKGEKVDFKSSWYSDRDYAELLGEDLKIR
                     DHIEKKLNRAGIADIQIRKAAEQGEVAVDIHTARPGIVIGKGGSEVDALRGDLERLTG
                     KKVQVNVREVSRPELNASLVAESIAEQLVGRAAFRRTMKRALTSAIRSGAEGARIQLG
                     GRLGGIEMSRSETISEGRVPLHTLDADIDYGFREAKTQMGQIGVKVWINHGVHDEQPE
                     AIRR"
     gene            complement(3865..4230)
                     /gene="rplV"
                     /locus_tag="VFT03_05230"
     CDS             complement(3865..4230)
                     /gene="rplV"
                     /locus_tag="VFT03_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU4847579.1"
                     /translation="MKAKAKYVRIAPRKARLVADEVRGKSYPEAVSLLRFTNKRAAKI
                     IGEIVQSAAANAEHNDDADPDELFIREIRVDDGPTIKRYRARAMGRATMIRKRTSHIS
                     VELGAPAGVLMGTPGDEEE"
     gene            complement(4227..>4371)
                     /locus_tag="VFT03_05235"
     CDS             complement(4227..>4371)
                     /locus_tag="VFT03_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HEU4847580.1"
                     /translation="HTIAVHDGRKHVPVYCTESMVGHKLGEFAPTRTFRTHLKGDRTV
                     RRR"
BASE COUNT          625 a         1529 c         1240 g          977 t
ORIGIN      
        1 ggatgggagt agccgacctg cagattgatg tcgcttccct gcagggccgc tctgtagccg
       61 acgccggcga tgttcagcgt cttggtgaac ccttccgtca cgcctgttac ggcgttgtag
      121 atcagggatc gcgtaagccc gtgcatcgcc ctgtgctcgg gcgtgtcccc ggcccgctcg
      181 actattacgt taccgtcttc ctgccttaca tcgataccgt gccccacggg caccgtgagc
      241 tcgcccttcg ggccgttcac ctttacgcta cggcccgaga gctccacgct gaccgtgcca
      301 gggatctcga caggagcctt cccgattctc gacactcctc aatacctccc tagtagacga
      361 agcaaaggac ttcgccgccg acgcccctgc gcctggcctg gtggtctgtc aggatgccct
      421 gggaggtcga gagtatcgcc accccgagcc cgtcgagcac gcgggggatg ttggtctgct
      481 tgcggtagac ccggcgtccg gggcggctca tccgcctcag gcctgcgatg ccgcggctac
      541 cgtcggggcc gtacttcaag gctattacga gcctcttttc gaccccgctc ccgcgctcgg
      601 agacgtcctg aacgtagcct tcctccagaa ggattcggga gatctccgcc ttcatcttcg
      661 agtgcggtat ctcgaccgtc tcctgcttga ccatgtgcgc gttgcggata cgcgtcaaga
      721 aatccgctat aggatcggtg accgtcatct tctaccaact cgctttcgtc acgccaggga
      781 tcttcccctc gtgcgccagc tccctgaggc agatccggca aagcccgaac ttcctgtagt
      841 aaccgtgcgc ccggccgcac ctctggcaac ggttgtactc cctgacggcg tacttctgct
      901 ttttctgctg ctttaccagc aacgcctttc tcgtcatttc ctacctctca actctgcctg
      961 aacggcatcc cgaggagccg caagagctcc cgcgcctctt cgtcgctctc cgtcgtcgtc
     1021 acgatcgcca cgtcgagtcc gcgggtcgcg tctatagagt cgtaggatat ctcggggaag
     1081 ataacctgct ccctgaggcc tatcgcatag tttccacgcc cgtcgaagga ggcggggcta
     1141 acacccctga agtccctgac acgcggtagg gctatggaga taaagcggtc gaggaactcc
     1201 cacatgcgct cgccgcgcag cgtgaccctg gccccgacgg gcatgccctc cctgatcttg
     1261 aacccggcga tactcttgcg cgcccgcctg agctgggcct tctggcccgt gatcctggcg
     1321 aggtcctcca tggcgccgtc gagcgcgcgg ctgttcacca cggcttcccc gacgcccatg
     1381 ttcaccacga tcttctcgag accaggcatc cgcatggggt tctcgatgcc gaacctctcc
     1441 tcgagatccg ggaccacctc ttcgtggtac ctttccttca acctagccat cgatatcctt
     1501 ccctgacttc ttcgagatcc ggagcttctc ccccgagtcc gtaaagcggt agcccaccct
     1561 cgtcggctcg cccgagttcg ggtcgacgag catcacgttg gagacgtcga tgggagcctc
     1621 gaagtgatag agcccttgct gattctgctg gctcggcctc gcgtggcgcg tgcggacgtt
     1681 gaccccgccg acgacgaccc tgttctcttt cgggatgact cgttcgatct cgccgcgttt
     1741 gcccttctct ttgcccgaga tcacaacgac ggtatctccg cgctttagtt tcagacccat
     1801 gattacaaca cctccggggc gagcgagatg atcctggtgt aacccttctc cctgagctcg
     1861 cgcgcaacgg ggccgaagat acgcgtcccg cggggagcgc catcggggtt gatgatcacg
     1921 ccggcgttct ccccgaagcg gatgtatgac ccgtcgtcgc ggcgcgtctc tttcttggtc
     1981 cgcaccacga cggcccttac cacgtcgccc ttcttgacgg cgctgccagg cgtggcctcc
     2041 ttgacggtgg ccacgatcac gtccccgacg ccggcactgc gccgcttgct tcccccgagc
     2101 acgcgaatgg taaggatact cctggccccc gagttatctg cgactctgag cctcgattcc
     2161 tgctgtatca ctactcggcc ctcgatatga tgttcgcgag ccgccacctc ttgcgggccg
     2221 agagcggcct ggtctcaacg atccgcaccg tatcgccaac gcgggcctcg ttgcgctcat
     2281 cgtgcaccat gaacttcctg gaacggcgca cccgcttctt gtacatcggg tggcgcacca
     2341 gcgcctcaac cgagacggtc accgtcttat ctgccctgtc cgagacgatg agcccgaccc
     2401 gctccttgcg cctgttccgc tcggtgtcgg tctggatctg gccctcgtct tggtccagcg
     2461 cggggccctt ggcaggaccg gcatcgaggc cggttactcc agcctcgatc aggtcctgtg
     2521 gcgtctcggc cacgggctcc tcggccttcc caggagtctg gtttttctct tcctctacca
     2581 ctacctactc tcctgctttc tggttcagga ccgtcaggat cctggcgata ttcttcctga
     2641 cctctttgag ctgtcccgtg ttctccaact gcccggtcgc gtgctggaag cgcaggttga
     2701 agagctcgcg ccttgtggcg gcgaggcgct gctcgagttc ctcgacgtca agctcgcgca
     2761 cttcggctgc cttcactgct gaccaccccc tcgagtaagg aagcgcgtct ttatggggag
     2821 cttctgggcg gcgaggtgca tggcctcgcg ggccagcgtc tcgtccacac cgctcatctc
     2881 gaacatcaca cgccccggct tgacgacggc gacgtacgcc tccgggctgc ccttgccact
     2941 acccatcctg gtctcggccg gcttcttggt cacgggcttg tgggggaaga cgttgatcca
     3001 gaccttcccc ccgcggcgaa tcttcctggt catcgcgata cgggccgcct ctatctggcg
     3061 gttggtgatc caggccggct ccagggcctg cagcccgtac tcgccgaact gcacatcggt
     3121 accgcccttg gccttgcccc gcatcctgcc cctgtggggc ttcctgtact tgagcttttt
     3181 gggtactaac atagcctacc gcctgatcgc ttccggctgc tcgtcgtgta cgccgtggtt
     3241 gatccagacc ttgaccccga tctgtcccat ctgggtcttg gcctccctga aaccgtagtc
     3301 tatgtccgca tccagcgtgt gcagaggcac tcttccttct gagattgtct cggaccggct
     3361 catctcgatg ccgccgagcc tcccgcccaa ctggatcctg gcgccttcgg cgccgctgcg
     3421 gatagcgctc gtgagtgcgc gtttcatggt ccgccggaac gcggcgcgcc cgacgagctg
     3481 ttcggcgatg gactcggcca cgaggctcgc attgagctcg ggacgggaga cctcgcgtac
     3541 gttgacctgc accttcttcc cggtgagcct ctcgaggtcg cccctcaggg cgtccacctc
     3601 gctgccgccc ttgccgatta cgatccctgg ccttgccgtg tggatgtcca cagcgacctc
     3661 accctgctcg gcggccttcc ttatctggat gtctgctata ccagccctgt tcagcttctt
     3721 ctcgatgtga tcccggatct tgaggtcctc gccgagcagc tcggcatagt ccctgtccga
     3781 ataccaactg ctcttgaaat ctaccttctc gcccttgcgc tggacgccga ggcggaagcc
     3841 ttcaggatgt actttctggc ccatctattc ctcctcgtct ccgggcgtgc ccatgagtac
     3901 gccggcaggg gcgccgagct cgacgctgat gtggctggta cgcttgcgga tcatggtcgc
     3961 ccggcccatc gccctcgcgc ggtaacgctt tatggtcggc ccgtcgtcca ccctgatctc
     4021 cctgataaac agctcgtcag ggtccgcatc gtcgttgtgc tctgcgttgg ctgcggcgct
     4081 ctggactatc tcgccgatga tcttcgccgc ccgcttgttg gtaaagcgca agagcgagac
     4141 cgcctcgggg tagctcttgc ccctgacctc gtctgccacg aggcgcgcct tgcggggggc
     4201 gatacggacg tatttggcct tcgccttcat cggcgcctca ccgtcctgtc gcccttgagg
     4261 tgcgtgcgga acgtccgggt cggggcgaac tcgcctagct tgtggcctac catcgactcc
     4321 gtgcagtaca cgggcacgtg cttgcgcccg tcgtggacgg cgatggtgtg g
//
LOCUS       DASTUV010000260         7503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1295316, whole genome shotgun sequence.
ACCESSION   DASTUV010000260 DASTUV010000000
VERSION     DASTUV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295316"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..920)
                     /locus_tag="VFT03_05240"
     CDS             complement(294..920)
                     /locus_tag="VFT03_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU4847581.1"
                     /translation="MAVAGRQPLSRRRVLEEAVRFADREGLEALTMRKLGAELGVEAM
                     SLYNHVPNKGALLDGMVEVLLGELEIPLRDQDWEERIREGYRAFRRLAHEHPHVFPLL
                     VNRPPETMDGVWLVEEFLQTLRDAGFGREEALHAFRVLSSYTFGYAMAEIRGFALEPD
                     GSRPGARALSPEEFPNLSELVPRLERVDHDAEFEFGLDLLLSGLRDRL"
     gene            1121..2878
                     /locus_tag="VFT03_05245"
     CDS             1121..2878
                     /locus_tag="VFT03_05245"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEU4847582.1"
                     /translation="MDDLGQESLDERLERLERDVEELRREVRGRVATVTSGRGTESGG
                     GHDFSGFTVSESGTIRRFRLPFELRQIRDLSSGEWWLNKLGIGLLLFGVAFLFIYSVE
                     RGWIGPWTRVAFGVAIGAVLLVVGLHVYGRRRAFGQVLLGGGVGALYITVFAAFQLYS
                     LVPYLAAFGFMVAVTLLAFVLSLRQDGASISLIGALGGLGTPFLLYTDSGSLGGLVLY
                     TCLILAGTGAVYYRKGWVSLLAVSFVGGWLVLLIGYADSAFLVVEPSLADRRVLQVGV
                     AFAWLLLWLVPVVRGITADEHRVSTQLFVISTPIVSFAFTAAIWDLPASEFGWIALSA
                     AVLYALAAFVLRRLEGSSRNLSYIHALVALMLLTLAAILMLEGDALIFTLAASAAILH
                     YVARRFSDGFISVEAHLIFFAVAVWLGARLVVPEGSFEAAGRAVLDTSALVDLLVIGL
                     AFAASLVVMPLSLGRIYRIAAHAAVLAWLWRELSALPGGEAYVTIAWGLYAVVLLVAG
                     LRLDRSALIRVAMATLFLVAGKLFLVDLAEVEALWRVLLFLGFGVLFLSLSYYLQALW
                     RPGRSTAEESGPHEGGERV"
     gene            2856..3974
                     /gene="lgt"
                     /locus_tag="VFT03_05250"
     CDS             2856..3974
                     /gene="lgt"
                     /locus_tag="VFT03_05250"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU4847583.1"
                     /translation="MKVGSGYEPGAVFEGGSRASGYRGVADIVGARRRAAFRVTGCGA
                     VDPVLADVGPVRLWYYGLAYAVGLLTVDLWVWLKRDSLGFESRDVAGFSVFFAAGVLL
                     GGRLFDVVLYEWFYYENHIWQVPDLSQGGMATHGVLLGAVMGSLLFCRLSGKSFLAVA
                     DEIVFPGAVLMALGRIGNHINGEVYGSVTDVAWAMEFPYAEGCRHPVALYDGLKNLLL
                     VPILLNVRSINLRRSGYLPRGLMLGHFVLWYGLLRVLVDFFRDYDSYWFGIGRGQYFN
                     LVMAMVGLCIVVVTRARSESGPVRRSAGMRDPAFWCKKVAFYALVALCFTIPSGWTQQ
                     VLDEFQARRDVASPVVTICGSERSYLTRRRRPSASGAQ"
     gene            complement(3934..4215)
                     /locus_tag="VFT03_05255"
     CDS             complement(3934..4215)
                     /locus_tag="VFT03_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847584.1"
                     /translation="MEPPITQATAHGNNVVAVYADRVELRSGWQGQHVEPVSIKDVSA
                     VRIRGLINCTLTILDNAGRIYELERMALPDARGVKSAIERQKQKAGVYE"
     gene            complement(4349..5731)
                     /locus_tag="VFT03_05260"
     CDS             complement(4349..5731)
                     /locus_tag="VFT03_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU4847585.1"
                     /translation="MLVLALPGLALAQDEPTVKALSLAMDTVWVLVAAVLVLFMQAGF
                     AMLEVGFSRMKNVGSVVAKILVNLAIAALLFWAVGFAIAFGTGNAIMGTQGWFLNVPA
                     SAVNDVYAGLSWTQVPLSAKFLFQVAFCAVSLAIVWGTMLERTKFAVYVIFAVVFAGL
                     IYPLVGHWIWGGGWLTELGMQDFAGSTVVHLSGAMAALAGTLLLGPRLGKYDDEGRPV
                     TIPGHNMPLAVLGVLILWVGWYGFNPGSTMAATPQIADIALTTTLAAAAGVLGAMGMS
                     YVYRRNVDVGMGGNGAIAALVAITAPCAFVAPWASIVIGFVAGVVMYATLVYVDRIGV
                     DDPLGAIAAHGMGGIWGTLSAGLFTTPELAAIGRPGLFYGGGLYQLGIQALGIVAAGG
                     FVFLVSLTVFAILKATIGIRVKPDQELDGLDIHEHGVYGYPDLVATDHQNGNGSSISD
                     PVQRPAGGAA"
     gene            complement(6012..>7503)
                     /locus_tag="VFT03_05265"
     CDS             complement(6012..>7503)
                     /locus_tag="VFT03_05265"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4847586.1"
                     /translation="IVWRSGGEPVPEAAQLAAALGLGLFVVPPEVPLRRLLSAFVAGD
                     GLLLLSHGVGRTLLESAGAEDTIGDLTARISALLGRPIVVEDPVGRLISSSPPADSEE
                     PNALRALLEKHNVRASKGSGVEETRRERRDRYARLPDGYLSVPVERWRMADEELHWSP
                     VGTGAPAGYLWMDLTEKSLRPEDVVLLYWARRVLETELGKERIRLETQLGVRGDFVDD
                     LINNQYGSVELLLQRAGYLDADLSEGALVLVVDIDDFARYLERRKPKEPVIQELKRRL
                     ADAVRLQTRQLFSNFLLGPRSDNVILFLGPSDTETPEELPELAQRLARNVQRYAKGLL
                     PDLTVSVGIGRYKSNPALLPDAYSEAEVALEIGHRINGPSSVSTFEGTGTYKLLFRVL
                     QENPEELEAFYGETLEPVVRYDSRYGTELVHTLTTYLDNDASTVKTATDLFAHRHTIR
                     YRLDRVGELTGLDVDKSEDREQLTLGIKAMQLLGRVPKRPSSFSDR"
BASE COUNT         1201 a         2376 c         2489 g         1437 t
ORIGIN      
        1 agccgctggg atgagtccgg cgccgagctt cgaagatggc taccgctgcc aggccgtgct
       61 cgacgctgtg gagcgcagcg tcgagagcag gagctggaca cagcctgcgg gcagcgacgc
      121 cacggtctag gctagaccgg tggcacgctt tcgctactcg tacgtcatct ccggtggctc
      181 tgccgaagag atatccgacc ggatagaccg gctgacgagc ctgggctacg acgccgtcga
      241 gcttggggcc gagttggcgc tggcagaggc gggcgaggcc agagacccct ctgtcagagc
      301 cggtcccgca ggccggacag taggaggtcc aggccgaact cgaactccgc gtcgtggtca
      361 accctctcta gccggggtac cagctcgcta aggttgggga actcctcagg cgagagcgcc
      421 cgcgcgccgg ggcgggaacc gtcgggctcg agcgcgaagc cccggatctc cgccatcgcg
      481 tacccgaagg tgtagctcga gagcacacgg aaggcatgca gcgcctcctc ccgcccgaaa
      541 ccggcatctc gcagcgtctg caagaactct tccacgagcc ataccccgtc catcgtctcg
      601 ggcggccggt tgacgagcag cggaaagacg tgggggtgct cgtgcgcgag ccgccggaac
      661 gcccggtacc cctcccggat gcgctcctcc caatcctgat cgcgcagcgg aatctccaac
      721 tcgcccaaca gaacctccac catcccatcg agcagcgcgc ccttgttcgg gacgtggttg
      781 tagagcgaca tcgcctctac cccgagctcc gctcccaact tcctcatcgt tagcgcctca
      841 agcccctcgc ggtccgcgaa ccgtacagcc tcctctaaaa cccgtcgccg actcaaaggt
      901 tgccgccccg ctacggccat ccgccaacct ccctcgtaaa tccacttgac aagcttacag
      961 tataagtgtt aaccttacgt tgtaaactta cagtgtaaat atacctatgc gcatggaggc
     1021 gaatggtctg aggattcgtg tataagcggc ttggggatct cggtttccgg ggcgggcagg
     1081 cagagggtag agggatcgtc ggcgtcaagg agtgaagagg atggatgatc tcggacaaga
     1141 gtcgctcgat gagcgtctgg agcgtctgga gagggatgtc gaggaattga ggcgcgaggt
     1201 gcgcggtcgc gtggctacgg tgacctccgg ccggggtacg gagagtggtg ggggtcacga
     1261 tttctccggc ttcaccgttt ccgagtctgg aacgattcgg cgattccggt tgcccttcga
     1321 gctgcggcag atcagggatc tgagcagtgg ggagtggtgg ctcaacaagc ttggcatcgg
     1381 gctcctgttg ttcggcgtcg ccttcctgtt catctattcc gtggagcggg gttggatcgg
     1441 tccgtggacg cgggtcgcgt tcggcgttgc gatcggggca gtgctgctgg tcgtggggct
     1501 acacgtctac gggcgacggc gggcctttgg ccaggtgctg ctcgggggtg gggtcggggc
     1561 tctgtacata accgtcttcg ctgctttcca gctctactca ctcgtcccgt atctggccgc
     1621 cttcggtttc atggtggctg tcacgctcct cgcgttcgta ctctccctgc ggcaggatgg
     1681 ggcatctatc tcgctcatcg gagccctcgg gggtctcggc acgccgtttc tcctgtacac
     1741 cgattccggg agcctcgggg gtctagtcct ctacacctgc cttatcctcg cgggcacggg
     1801 ggccgtttac tatcgaaaag gttgggtctc gctccttgcc gtctccttcg tgggcgggtg
     1861 gttggtctta ctgatcggct atgctgacag cgcctttctc gtagtggaac cctctctcgc
     1921 cgaccggcgg gtcctgcagg tcggcgtggc cttcgcgtgg cttctcctgt ggctcgtgcc
     1981 ggtcgtgcgc gggatcacgg cggacgagca tcgcgtatcg acccaactgt tcgtgatctc
     2041 gacgccgata gtgtccttcg cgttcaccgc ggcgatctgg gatctgccgg cctctgaatt
     2101 cggctggatc gcgctctctg cggcggtcct ctacgcgctc gccgccttcg tgctgcgaag
     2161 gctggaggga tcctcccgaa acctctcgta cattcatgcc ctcgtggctc tgatgctcct
     2221 cacgctcgcc gcgatcctga tgctcgaggg cgatgcgctt atctttaccc tcgccgcctc
     2281 ggcggccatt ctccactacg tcgccagacg attctccgat gggttcatct ctgttgaggc
     2341 gcatctcatc ttcttcgccg tcgccgtctg gctcggcgcc cggctcgttg ttccggaggg
     2401 ctccttcgag gctgccggac gggcggtgct cgacacctct gcgctagtgg acctgctggt
     2461 catagggttg gccttcgccg cgtcgctggt ggttatgccg ctgagtctgg gcaggatcta
     2521 ccggatagcg gcacacgccg ccgttctcgc gtggctctgg cgcgagctct cggcgctgcc
     2581 gggcggcgaa gcatacgtca ctatagcctg ggggctatac gccgtcgtgc tccttgtcgc
     2641 cgggctccgg ctcgatcgga gcgcgctcat cagggtagcc atggcgacgc tattccttgt
     2701 ggctggcaag ctgttcctcg tagatctcgc ggaggtagag gcgctctggc gcgtacttct
     2761 ctttctcggg tttggcgtcc tcttcctctc cttgagttac tacctgcaag ccctctggcg
     2821 tccaggacga agcactgcgg aggagtccgg tcctcatgaa ggtggggagc gggtatgaac
     2881 cgggagcggt gtttgagggg ggcagccggg catccggcta ccgcggcgtg gccgatatcg
     2941 tcggcgctcg cagacgcgca gcgttccgcg taacggggtg cggagccgtg gatccagtgc
     3001 tcgcggatgt cgggccggtc aggctctggt attacgggct ggcgtacgcc gtcgggctcc
     3061 tgaccgtaga cctctgggtg tggctgaagc gggacagcct ggggttcgag agccgtgacg
     3121 tcgccgggtt ctcggtgttt ttcgccgcgg gcgtgttgct cgggggcagg ctctttgacg
     3181 tggtcctcta cgagtggttc tactacgaga accacatctg gcaggttccg gacctctcgc
     3241 aggggggcat ggcgacccac ggggtcttgc ttggggccgt catggggtcg ctgctcttct
     3301 gccggctaag cggcaagagc ttcctcgcgg tcgcggacga gatagtgttc cccggcgccg
     3361 ttctcatggc gcttggacgg atagggaacc acattaacgg agaggtctac ggatcggtta
     3421 ccgacgtggc ctgggcgatg gagttcccct acgccgaggg gtgcagacac cccgtcgccc
     3481 tctacgatgg gctcaagaac ctactgctcg taccaatcct gctcaacgtc cggagcatca
     3541 atctgcgccg gagcgggtac cttccgcgag ggctcatgtt agggcacttc gtgctctggt
     3601 acggccttct gcgggtgctc gtggattttt tccgggacta cgattcctac tggttcggca
     3661 tcggcagggg ccagtacttc aacctggtca tggccatggt gggactttgc atcgttgtcg
     3721 tcacccgcgc caggtcggag tcggggccgg tgcgacggag cgcggggatg cgagacccgg
     3781 cgttctggtg caagaaggtg gccttctacg cgctggtggc tctctgcttc accatcccga
     3841 gcggatggac gcagcaggtc ctcgatgagt tccaggcgag gagagatgtt gcttccccgg
     3901 tagtaacgat atgtggtagc gagcggagct atctcactcg tagacgccgg ccttctgctt
     3961 ctggcgctca atagcggatt ttacgccgcg ggcgtcgggg agggccatgc gttccagctc
     4021 gtagatgcgg cctgcgttgt ccaggatagt gagggtgcag ttgatcaggc cccgtatcct
     4081 gaccgccgag acgtccttga tgctcacagg ctcgacgtgt tgcccctgcc acccgctgcg
     4141 caactctacc ctgtcggcgt agacggcgac gacgttattc ccgtgcgccg tcgcctgggt
     4201 tatcggtggt tccaaaggtt ctccgtcctg ttcgtgccgc ctccgaaatc cttcacgcgt
     4261 acgcggaaga accccgaaga cgtgttcccc ggggccttcc gctgcaaaca ccggccgggt
     4321 tacccggccg gtgtggttgt ctttcgtcct acgccgcgcc ccctgccggt ctctgcaccg
     4381 ggtccgatat actgctgccg ttcccgttct ggtggtcggt ggcgacgagg tcggggtaac
     4441 cgtagacccc gtgctcgtga atgtccaggc cgtcgagctc ctggtcgggc ttgacccgga
     4501 tgccaatggt cgccttgaga atcgcgaaca ccgtcagcga aacgaggaag acgaagcctc
     4561 ccgcggccac gataccgagg gcctgaatgc cgagctggta gagcccgccg ccgtagaaga
     4621 ggccgggccg gccgatagcc gcgagctccg gggtggtgaa gaggccggcc gatagcgtgc
     4681 cccagatgcc gcccatcccg tgggccgcga tggctccgag cgggtcgtcg actcctatcc
     4741 tgtctacgta gacgagcgtc gcgtacatca cgacgccggc gacaaagccg attacgatag
     4801 acgcccacgg ggcgacgaag gcgcagggcg cggtgatggc gacgagcgcc gcgatcgcgc
     4861 cgttgccgcc catcccgacg tcgacgttgc gcctatatac gtagctcatg cccatcgcgc
     4921 cgagcacgcc ggctgcggcc gccagggtgg tcgtgagggc gatgtccgcg atctgtggag
     4981 tcgccgccat cgtcgagccg gggttgaagc cgtaccagcc gacccacagg atcaggacgc
     5041 cgagcacggc gagcggcatg ttgtgaccgg ggatggtcac ggggcgtccc tcatcgtcgt
     5101 acttgccgag gcgaggtccg agaagcaggg tccccgccag cgccgccatc gcgcccgaga
     5161 gatggacgac cgtcgagccg gcgaagtcct gcattccgag ctcggtcagc cagccaccac
     5221 cccagatcca atgccccacc agcgggtaga tgaggcccgc gaagactacc gcgaagatca
     5281 cgtacaccgc gaacttggtc cgctcgagca tcgtgcccca gacgatcgcc agggacacag
     5341 cgcagaaagc tacctggaag aggaacttgg ccgagagcgg tacctgggtc cacgagagcc
     5401 cggcatacac gtcgttgacc gcgcttgccg gcacgttgag gaaccagccc tgcgtcccca
     5461 tgatggcgtt gccggtgccg aaggcgatcg cgaaccccac agcccagaac aacagggcgg
     5521 cgatcgcgag gttcacgagt atcttcgcga ccacgctccc gacgttcttc atccgcgaga
     5581 agccgacctc gagcatcgcg aaccccgcct gcatgaacag caccaggacc gccgctacta
     5641 gaacccacac ggtgtccatc gccagcgaga gagccttgac cgtaggctcg tcctgcgcga
     5701 gcgccaggcc aggtaacgcc agcaccagca tgagaaccat agctgttgat aaaccaagcc
     5761 tccgcaaaag cgttctcctt cgaagtcggg tacttactct acgaacatag tctaggttcc
     5821 ccctccccaa agggtcttta gccgagtgta caaagatggc cgtgcgcccg gggcggccct
     5881 tgctctgctt gtacaaagaa ggtttcaggc atcaggatct ttcagcggta ctgcgcgccg
     5941 tgatccagga ctacgatggc gggggcaacg cacgacgcac ggtcttgggt ctatccaatc
     6001 cctgctgaag cctacctatc cgagaagctc gaaggtcgct tggggacgcg gccaaggagc
     6061 tgcatggcct tgatcccgag tgtcagttgt tcgcggtctt cgctcttgtc cacgtcgagc
     6121 cctgtgagct cgccgacgcg gtcgaggcgg tagcggatgg tgtggcggtg ggcgaagagg
     6181 tctgtcgcgg tcttgacggt cgaggcgtcg ttatccaggt aggtggtgag ggtgtggacg
     6241 agctccgtgc cgtagcgcga gtcgtagcgg actactggct ccagggtctc gccgtagaac
     6301 gcctcgagct cctcagggtt ctcctgcagg accctgaaga ggagcttgta ggtgcccgta
     6361 ccctcgaagg tcgagaccga ggaagggccg ttgatgcggt gccctatctc cagggccacc
     6421 tccgcttcgg agtacgcatc tggaagcagg gccgggttgc tcttgtagcg cccgatcccg
     6481 acggaaaccg ttaggtcagg cagcagcccc ttggcgtagc gttgcacgtt cctcgccagc
     6541 cgctgcgcca gctcggggag ctcctcaggc gtctcggtgt cggaagggcc gaggaagagt
     6601 atgacgttat cggagcgggg accaaggagg aagttagaga agagctggcg ggtctggagg
     6661 cgcacggcgt ctgccaggcg ccgcttgagc tcctgtataa ctggctcctt cggcttgcgg
     6721 cgctccaggt agcgggcgaa gtcgtctatg tcgaccacga ggacgagcgc gccctccgag
     6781 aggtcggcgt cgaggtaccc ggcgcgctgg agcaggagct cgaccgagcc atattggttg
     6841 ttgatcaggt catccacgaa atcgccccgg acgccgagct gggtctccag gcgtatccgc
     6901 tccttgccga gctcggtctc gagcacgcgc ctcgcccagt acaggaggac gacgtcctcc
     6961 ggtctcaggg acttctctgt gaggtccatc cacaggtagc ccgcaggggc gccggtcccg
     7021 accgggctcc agtggagctc ctcgtccgcc atccgccagc gctcgaccgg cacagagagg
     7081 tagccgtcgg gaagccgggc gtagcggtcc ctccgctcgc ggcgcgtctc ctccaccccc
     7141 gaccccttcg aggcccgcac gttgtgcttc tcgaggagcg ctcgcagggc gttcggctcc
     7201 tctgaatccg cgggcgggga actagagatc agacgcccca cagggtcctc gacgacgatg
     7261 ggacggccga gcagcgcgga gatgcgggcc gtgaggtccc cgatggtatc ttcagcgcca
     7321 gcgctctcga ggagggtccg gcctactccg tgggagagga gcaagagtcc gtcgccggcc
     7381 acgaaggccg agagcaaccg ccgcaacggc acctccggcg ggacgacgaa gagcccgagc
     7441 cctagtgccg ccgcgagctg cgcggcctcg ggcaccggct cgccgccgct gcgccacacg
     7501 atg
//
LOCUS       DASTUV010000261         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_945144, whole genome shotgun sequence.
ACCESSION   DASTUV010000261 DASTUV010000000
VERSION     DASTUV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945144"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFT03_05270"
     CDS             <1..1243
                     /locus_tag="VFT03_05270"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEU4847587.1"
                     /translation="SGVAMSTFWHSVLGLDRDGRPTTPILTWADRRAAECVPELRERL
                     DEKATHRRTGCVPHSSYWPAKLLWSSCVMPEAFEKTDRWVSPADYFYARFFGEPYQVG
                     TSMASGTGLFDQNRRRWDHETLEALPAEEAQLSAISDEPRRGLAGEWTERWPALRETP
                     WLPAVGDGACSNVGSGCTGSDRLALMVGTSGAMRVLWKAGSVEIPDGPWCYRADAERY
                     VMGGALSDGGNLIEWLRNTLRLPHPEKTEELLSDMEPDSHGLTFLPLLAGERGPGWAD
                     RANGTVSGLSMTSKPVEILRAAMEAVAFRFAIIAEMIETASPGEKEVVASGGGLLNSP
                     TWTRIMADTLGRPVTLSGVKEASSRGAALISLEALGGPEIETAQAPLGETFEPDPSSH
                     EIYMRALERQRRLYDAVLGGS"
     gene            complement(1430..>2146)
                     /locus_tag="VFT03_05275"
     CDS             complement(1430..>2146)
                     /locus_tag="VFT03_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU4847588.1"
                     /translation="DNDLSYAGNFLAMLFKMAEPKYEPDPRLEKALDVLFILHADHEQ
                     NCSAAAVRVVGSSRVDPFSAITAGCAGLYGPLHGGANVAVLRMLQRVGSIDNIPAFLQ
                     GVKQGDERLMGFGHRVYKNYDPRARIIRGHVDEVLEATNKESPLLDIAIELEKQALDD
                     DYFTSRKLYPNVDFYSGLIYEALGIPTDAFTVMFAIPRTSGWMAQWMEMIDDPELKIA
                     RPRQIYTGPRETDYVPIDER"
BASE COUNT          382 a          657 c          723 g          384 t
ORIGIN      
        1 ctccggcgta gccatgagca ccttctggca ctcggttctc ggcctcgacc gggacgggcg
       61 tcctacaacc ccgatcctta cctgggccga ccgccgcgcg gcagagtgcg taccagaatt
      121 gcgcgaacgc ctggacgaga aggccaccca ccgtcgaacg ggttgcgtgc cccactccag
      181 ctactggcct gccaagctgc tgtggtcgag ctgcgttatg cccgaggctt tcgagaagac
      241 ggaccgctgg gtctcccccg ctgactactt ctacgcccgt ttcttcgggg agccgtatca
      301 ggtgggtacg tcgatggcct cgggaacagg cctcttcgac cagaaccggc ggaggtggga
      361 ccacgagacg ctggaagcct tgccagccga agaggcgcaa ctctccgcca tctcggatga
      421 gccccgaagg ggtctggcag gggagtggac agagcggtgg ccggccctgc gggagacacc
      481 ctggctcccc gcggtgggcg acggtgcctg ctcgaacgtc ggaagcggat gcacgggtag
      541 tgaccggctg gcgttgatgg tcggcaccag cggcgcgatg cgcgtactgt ggaaagcggg
      601 gtctgtcgag atacccgatg gtccctggtg ctacagggcc gacgcggaac gctacgtcat
      661 gggcggcgcg ctctcagatg gcggaaacct gatcgaatgg ctgcgcaaca cgctacgcct
      721 tccacatccg gagaagaccg aggaactgct gtccgacatg gagccggact cccacggcct
      781 cacgttcctg ccgctgctcg ccggagagcg tggaccggga tgggccgaca gggccaacgg
      841 gaccgtgtcc gggctctcga tgaccagcaa gccagtcgag atactgcggg ccgcgatgga
      901 ggctgtggcc ttcaggttcg ccattatcgc tgagatgatc gaaaccgctt cccccggtga
      961 gaaggaggtc gttgccagcg gcgggggcct cctcaactca ccgacgtgga ccaggatcat
     1021 ggcagacact ctaggcagac cggtcacgct ctctggtgta aaggaggctt ccagcagggg
     1081 cgcagccctg atctccctgg aagcgctggg agggccggag atcgagaccg cacaagcgcc
     1141 actcggcgag accttcgaac cggacccgtc cagccacgag atctacatga gggcgctcga
     1201 acgccagcgc cgactctacg acgccgtgct cggcggctcg taattttcag catttcagca
     1261 cgcgccctgt cgggcgctcg tcagcacggc gcctgttcca ggcgacttgt cagcatgtca
     1321 gctttcgcgt gcgcgagctc ttgctgactg gcagagaagg ggcgcagccg ccgtgctgac
     1381 aagtggaggc gtagccggag cgtgttgaaa tgccgccgat agacgagcgc tagcgctcgt
     1441 ctatcggcac gtagtctgtt tcgcgggggc cggtgtaaat ctggcgcgga cgggcgatct
     1501 tgagctctgg gtcgtctatc atctccatcc actgggccat ccaacccgag gtgcgtggta
     1561 tggcgaacat gactgtgaaa gcatcagtcg gtatcccgag ggcttcgtag atgaggcccg
     1621 agtagaaatc gacgttggga tagagcttgc ggctcgtgaa gtagtcatcg tcgagcgcct
     1681 gcttttcgag ttcgatggcg atgtcgagca gcgggctctc cttgttcgtg gcctctagaa
     1741 cctcgtcgac gtggccgcgg atgatcctgg cccgggggtc gtagttcttg tagaccctgt
     1801 gcccgaagcc catgagacgc tcgtcaccct gcttgacgcc ttggaggaag gccgggatgt
     1861 tatcgatgct tccgacgcgc tggagcatcc tgaggaccgc aacgttggcc ccgccgtgca
     1921 acgggccgta gagcccggcg cacccggcgg tgatggccga gaaggggtcg accctcgaag
     1981 acccgacgac ccttaccgca gcagcggaac agttctgctc atggtcggca tgcaatatga
     2041 agagcacgtc gagcgctttc tccaggcgcg ggtcgggctc gtacttgggc tctgccatct
     2101 tgaagagcat ggccaggaag ttgcccgcgt aggagaggtc gttgtc
//
LOCUS       DASTUV010000262         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1573962, whole genome shotgun sequence.
ACCESSION   DASTUV010000262 DASTUV010000000
VERSION     DASTUV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573962"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1500)
                     /gene="menE"
                     /locus_tag="VFT03_05280"
     CDS             complement(49..1500)
                     /gene="menE"
                     /locus_tag="VFT03_05280"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEU4847589.1"
                     /translation="MDDGAHVPCPLRSAARRSPDAPAIVGAGGSLAYGELDQRVSVAA
                     AKVRELGYGAGDRVALYLPKDERYLVLLLALIRTGCVACLLSTRLPPRGAAPLPERTA
                     CRALISTSEEILEAPGVRTLRPEDLLSGGTPKRDGSQTSKELWLALDRPATVVFTSGS
                     AGVPKAALHTFRNHYFSARGSNTNIALAPGDRWLHSLPLYHVGGLSIVFRCLLAEATV
                     VLPEPDVPLGEAVASATHVSLVSTQLLRLLREGGLEAGRLEAILLGGGPLPTSLVDEA
                     ADLGLPVHTSYGLTEMASQVTTTPPGASRKELHTSGRPLPHREVGISGDGEILVRGET
                     LFAGYVEGDAVDRPLDADGWFHTGDIGDLSENGYLRVLGRKDNLFVSGGENIQPEEIE
                     EALSSLAGVEVAVVVPFPDPEFGARPVAFVRMADGEVESETLARALEKVLPRFKIPVV
                     FHDWPEEAGLGEMKVDRAFFQERASQSRREVGG"
     gene            complement(1516..2367)
                     /gene="menB"
                     /locus_tag="VFT03_05285"
     CDS             complement(1516..2367)
                     /gene="menB"
                     /locus_tag="VFT03_05285"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEU4847590.1"
                     /translation="MTIIDWQTAGEFTDIKYHKAGGIAKITINRPEVRNAFRPLTVIE
                     MQRALEDAREDHEVGVIILTGEGTEAFSSGGDQKVRGDAGYLEAPDDPQRTPGGASVG
                     RFHVTDLHVQIRRCPKPVVAMVAGYAVGGGHVLHVICDLTIAADNARFGQVGPKVGSF
                     DGGFGASVLTQLVGPKKAKELWFLCRLYSAEEALEMGLVNEVVPLAELEAETVRWCRE
                     MLERSPFALRLLKASFHAHEDGFAGIQQLAHDANLLFYGSEEAREGRDAYKEKRKPDF
                     SQFPHRP"
     gene            2482..2901
                     /locus_tag="VFT03_05290"
     CDS             2482..2901
                     /locus_tag="VFT03_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4847591.1"
                     /translation="MHHQTYRSLANARDFMRHAYQHPINLPDISAQANLSPYHFLRVH
                     KLAYGETPHEFLTRLRIDRAKTLLARGSHNVTEACFEVGFSSLGSFSALFNQRVGLSP
                     SEYRRYVRSSVAVPRTVRPLFVPSCFLAMLRGPAKAR"
     gene            2978..>3181
                     /locus_tag="VFT03_05295"
     CDS             2978..>3181
                     /locus_tag="VFT03_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4847592.1"
                     /translation="MIQRQSHSTIFVTDQQEALEFYRDKLGFEVRTDMPMTDEGDIRW
                     LTLSPKGQPDLEIILAPLTPGPMF"
BASE COUNT          553 a         1069 c          996 g          563 t
ORIGIN      
        1 agaggaagct cccacagctt cttgagccca cgaggcggga ccttcgtgtc agcctcctac
       61 ctcgcggcgc gactggctcg cgcgttcctg gaagaatgca cggtccacct tcatctctcc
      121 gagccccgcc tcctcgggcc agtcatggaa tacaaccggg atcttgaacc tcggcaagac
      181 cttctcaagc gcgcgcgcca gcgtctcgct ctccacttca ccgtcggcca tccgcacgaa
      241 ggcgacggga cgggcaccga actccggatc ggggaagggc accaccacgg ccacttcgac
      301 gcccgcgagg ctcgagagag cttcctctat ctcctcgggc tggatgttct ccccaccgga
      361 gacgaagagg ttgtccttgc gacccagtac acgcaggtaa ccgttctcgc tcagatcccc
      421 tatatcccct gtgtggaacc agccgtccgc gtctagcggc cggtctaccg cgtcgccctc
      481 gacgtagccg gcgaagagcg tctctcctct caccagtatc tcgccgtcgc cggagatccc
      541 gacctccctg tgcggcaaag ggcggcccga cgtgtgcagc tcttttcgcg aagcgccggg
      601 cggcgtggtt gtgacctgcg aggccatctc tgtaaggccg tagctggtgt ggaccgggag
      661 gccgaggtcc gctgcctcgt ccacgaggga ggtggggagc ggtcctcctc cgagcaggat
      721 cgcctcgagc ctccccgcct ctaaacctcc ttctcgcagc aggcgcaata gctgggtgga
      781 gaccagggag acgtgcgtgg cgcttgcgac tgcctcaccc agcggcacat cgggctccgg
      841 caacaccacg gtcgcctccg cgagcaggca ccggaagacg atggagagcc ccccgacgtg
      901 atagagcggc agcgagtgga gccagcggtc gccgggcgcg agcgcgatgt tggtattcga
      961 gcctcgcgcg ctgaagtagt ggtttctgaa ggtgtgcagc gcggccttcg ggacacccgc
     1021 actgcccgag gtgaagacga cggtcgcggg ccgatccagc gcgagccaga gctccttgga
     1081 agtttgcgat ccgtcccgct ttggcgtccc gccggagagc agatcttcgg gacggagcgt
     1141 ccggacgccg ggtgcttcga gtatctcttc ggaggtggag atcagggccc ggcaggcggt
     1201 cctctccggc agcggcgcgg caccccgcgg cggaaggcgt gtgctgagga ggcaggcgac
     1261 gcagccggtc cggatcaggg cgagcagcaa tacgaggtag cgctcgtcct tgggaagata
     1321 gagcgcgacg cgatcccccg ccccgtagcc aagctcccgc accttcgccg ccgcgaccga
     1381 cacgcgttgg tcgagctctc cgtacgccag cgaaccaccc gcgccgacta tagccggagc
     1441 gtcgggcgaa cgccgcgccg cggaccgcag gggacagggt acgtgtgccc catcgtccat
     1501 cacttcacgc cgccttcagg ggcggtgggg gaactgcgag aagtccggtt tgcgcttctc
     1561 tttgtaggca tcgcggcctt cccgggcctc ctcgctcccg tagaagagga ggttcgcgtc
     1621 gtgggcgagc tgctgtatgc cggcgaaccc gtcctcgtgg gcgtggaagc ttgccttcag
     1681 cagccgcagc gcgaacggcg agcgttccag catctcccgg caccagcgga cggtctcggc
     1741 ctccagctcg gcgagcggta cgacttcgtt gacgagcccc atctccagcg cctcctctgc
     1801 cgagtacagc cggcagagga accataactc cttcgccttc ttcggcccga cgagctgggt
     1861 gaggacggag gcgccgaagc cgccgtcgaa gctgccgacc ttcggaccga cctgtccgaa
     1921 tcgggcgttg tcggcagcta tggtcaggtc gcaaatcacg tgcaacacgt ggcccccgcc
     1981 gaccgcgtat ccggcgacca tcgccaccac gggcttgggg caacgccgga tctgcacgtg
     2041 gaggtccgtg acgtgaaagc gccctaccga cgcgccgccg ggtgtgcgtt gcgggtcgtc
     2101 gggagcttcg aggtacccgg cgtcgccacg caccttctgg tctccacctg aggagaaagc
     2161 ctccgtaccc tctccggtaa ggatgatgac gcctacctcg tggtcttcgc gggcatcttc
     2221 gagagccctt tgcatctcga tgaccgtaag tggtctgaag gcgtttctga cctcggggcg
     2281 gttaatggtg atcttcgcga tcccccccgc cttgtgatat ttgatgtccg taaactctcc
     2341 ggctgtctgc caatctatga tagtcatggt aggaagatta tagggttcga agaagtcgtg
     2401 cagtcaatgc ggatacgaac gtcgcgcacc gccaaaaacc gtctttgatt cgatgggacc
     2461 cggctgtact cttgcttcga cgtgcatcat caaacttaca ggagcctggc caacgccagg
     2521 gacttcatgc gccacgctta ccagcaccct atcaacctgc cggacatttc ggcgcaggcg
     2581 aacctctccc cctaccactt cctgcgcgtc cacaagctgg catacgggga gacgccgcac
     2641 gagttcctga cgcggctgcg tatagacagg gcgaagaccc tgctggcgag ggggagccac
     2701 aacgtcaccg aggcgtgctt cgaggtcggc ttctccagcc tgggcagctt cagcgccctg
     2761 ttcaaccagc gcgtagggtt atctccctcc gagtaccggc gttatgtcag gtcgagcgtc
     2821 gcggtcccac gaacggtccg tcctctgttc gtcccctcat gcttcctcgc catgctccgc
     2881 gggcctgcca aagcgagatg agagcaattt tcgagaagca aaccgtatgc cgccggtgtt
     2941 atcttcaaag cgaagccacg acacagggag gtcagccgtg atccaacgcc agagccactc
     3001 gacgatcttc gtgacggatc agcaagaggc gctcgagttc taccgggaca agctcggctt
     3061 cgaggtgcgt accgacatgc ccatgaccga cgagggcgat atccgctggc tgacgctgag
     3121 cccaaagggg cagcctgacc tcgagatcat cctcgccccg ctgactcccg gacccatgtt
     3181 c
//
LOCUS       DASTUV010000263         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1682871, whole genome shotgun sequence.
ACCESSION   DASTUV010000263 DASTUV010000000
VERSION     DASTUV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682871"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFT03_05300"
     CDS             <1..450
                     /locus_tag="VFT03_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4847593.1"
                     /translation="RLPPLTVEESNALATTPVEELLSLPFLQPDQVTDSLHAYQLAKR
                     GNSLRVMAEAVRWGERDARVNTISPGIVMTPLAKDELGGPRGAGYRRMIEGSAAGRAG
                     TPDEVGTVGALLMGPDGGFITGSDFLMDGGVTAAYWYGDLAEVRAQQ"
     gene            complement(692..1483)
                     /locus_tag="VFT03_05305"
     CDS             complement(692..1483)
                     /locus_tag="VFT03_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847594.1"
                     /translation="MGRDRDADRGRPVEPGESRAHGNDPDAVEVRRASDREEQLRAEG
                     LGPDIPRRSEPASDRTRYEEVGVSGGTADHGARRLVSGHDDEGRAAARRDSIRWGPIW
                     AGLITALTVFLLLQLLAIGLGLIDIGPGAGGGGWASAIIGLIAFFIGGSVAGMTSAVR
                     GEATGLLNGFLVWALGTVLILLLSALGLGQIFGALGHVVGQLGVLQNLQGANVPDINP
                     ARIAQAVRSGAIGAFFGLLLSALASMLGGFLGGRSSDPLGRMARE"
     gene            complement(1571..>2450)
                     /locus_tag="VFT03_05310"
     CDS             complement(1571..>2450)
                     /locus_tag="VFT03_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEU4847595.1"
                     /translation="MEREQRTDRFTTLEDRLAGYEVYDPSGEKIGKVDDLFVDENDQP
                     EYIGVKMGFLGTSSTLIPVGIATIDSDRGFIEVSQPQSTVKDGPAFDDDREVTPEYEE
                     QVRSYYGLEGTQTSEKQGGYGAHYGDSQGEHPGEVGPGMMMGDTESGEFRGHGREQEG
                     LRQPGSDLEDEDELRVQRTEEELRAGTRERDAGALNVRKRVVTERERMEVPTRREEVT
                     VDRVPVEGEATEAEIGDDEVRVPVIEEEVVVEKRPVAKEEIRIRKDVVEDTEVVEEDV
                     RREEVEIDDQTTGRTR"
BASE COUNT          403 a          877 c          740 g          430 t
ORIGIN      
        1 cgccttccac cgctcaccgt cgaggagagc aatgcgttgg ccacgacgcc cgtggaagag
       61 ttgctcagcc ttccgttcct tcagcccgac caagtgacgg actccctgca cgcgtaccag
      121 ctcgcgaagc gcggcaactc gctgcgcgtg atggccgagg cggtgcgctg gggcgagcgc
      181 gacgcgcggg tcaacacgat cagcccgggc atcgttatga ccccgctggc caaggacgaa
      241 ctcggcggtc cgcgcggagc agggtaccgg cgcatgatcg aggggtccgc ggccgggcgc
      301 gccggcactc cagacgaggt gggaacggtc ggcgcgctgc tgatgggccc ggacgggggg
      361 ttcatcaccg gcagcgactt cctcatggac ggcggggtga ccgccgccta ctggtacggc
      421 gacctcgccg aagtgcgcgc gcagcaatga gcgtccaaca gaccgggtgg gcacggctgg
      481 ccgggaggac gatcgagacg cccggagagt aaccagcacc cgcgtcgcaa gcggagagca
      541 tcggcatcaa cttcgcagaa ctccgtcatg gcgaagttcg cagaatttcc ctttccacgc
      601 actcgggtga ataagggtaa gaagagaaag ggccggaatc ctatccgtaa gggctcaccc
      661 ctatggcagc cgagttaccg gccagtgact ctcactcgcg ggccatgcga cctagggggt
      721 cactactcct accccccagg aagcccccga gcatagaggc cagcgccgag agcagcaagc
      781 cgaagaacgc ccctatggcc ccgctcctaa cggcctgcgc tatccgggcg gggttgatgt
      841 ccggcacgtt cgcgccctgt agattctgga ggacacccaa ctgcccgacc acgtggccta
      901 aagcgccgaa gatctggccg agccccaggg ccgagagcag cagtatcagc acggtgccca
      961 aagcccatac caggaagccg ttcaagagcc ccgtagcctc gcccctcacc gccgaggtca
     1021 tgccggccac cgacccaccg atgaagaagg cgatcaggcc tatgatggcc gaggcccaac
     1081 cgccaccgcc ggcccccgga ccgatgtcga tcaggccgag ccctatcgct aagagctgca
     1141 gcagcaggaa gacggtaaga gccgtgatca gcccggccca gatcggcccc cagcggatgc
     1201 tgtcacgccg cgccgcggcg cggccctcgt cgtcgtggcc gctgacgagc cgccgggcgc
     1261 cgtgatccgc cgtcccacca gagacgccga cctcctcgta gcgcgtccga tcgctcgcag
     1321 gctcagagcg cctgggtatg tcgggcccca agccctccgc ccgaagctgc tcctcgcgat
     1381 cggacgcgcg gcgtacctcc acggcgtccg ggtcgttgcc gtgcgcccgg gactccccag
     1441 gctccaccgg cctgccccgg tctgcatctc tgtctctgcc catatcttct ctcctaaaaa
     1501 gatgagctcg gccgaaagcc gggcccaccg gttggtaaca gtgttgaggc ttgcggtatg
     1561 gtgctatccg ttaccgtgtg cgcccggtgg tctggtcgtc tatctcgacc tcctcgcgcc
     1621 ttacgtcctc ctccacgacc tcggtgtcct ctaccacgtc cttgcggatc ctgatctctt
     1681 ccttggccac gggccgcttc tccaccacga cctcctcctc gattacaggc acccgcacct
     1741 cgtcgtcgcc tatctcggct tctgtggcct caccctcaac agggacgcgg tctacggtta
     1801 cctcttcgcg acgggttgga acctccatcc tctcgcgctc ggttacgacg cgcttgcgga
     1861 cgttcagagc cccggcatcg cgctcgcggg taccggcccg aagctcttcc tcggtcctct
     1921 ggaccctcag ttcgtcttcg tcctcgaggt cgcttccggg ctggcgcaga ccctcttgct
     1981 cgcgcccgtg cccgcggaac tcaccgctct cggtgtcgcc catcatcatg ccgggaccca
     2041 cctcgccggg atgctctccc tgtgaatccc cgtagtgcgc gccgtagcct ccctgtttct
     2101 cggaggtctg agtgccttcg aggccgtagt agctacgaac ctgctcctcg tactcgggcg
     2161 ttacctcccg gtcgtcgtcg aaggccgggc cgtccttgac cgtgctctga ggctgggaga
     2221 cctcgataaa gcctcggtcg ctgtctatcg tggcgatgcc caccgggatc aaggtggaac
     2281 tggtgccgag aaagcccatt ttcacaccaa tatactccgg ctggtcgttc tcgtcgacga
     2341 agaggtcgtc gaccttgccg atcttctcgc cgcttgggtc gtagacctcg taaccggcta
     2401 accggtcctc aagagtcgta aatctgtccg tgcgctgctc gcgttccatc
//
LOCUS       DASTUV010000264         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_252008, whole genome shotgun sequence.
ACCESSION   DASTUV010000264 DASTUV010000000
VERSION     DASTUV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252008"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VFT03_05315"
     CDS             complement(<1..1415)
                     /locus_tag="VFT03_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU4847596.1"
                     /translation="MIRLPVREEKKARPAKKRPDTPPLTKGNMQVRVVTLAVVVAVVF
                     IVLAARLWYLQILTGTEYSVAAQATQTLEVKDPAQRGVIRDRDGQVLANNVPGLNVTV
                     TPSNISREKVKELAKILGADPKTVLESYDSAYDWGTQYSPMLVKENADRDGVTYVMER
                     TEEFPGVVVNDDYVRNYPAGDMAAHILGYTGAITQQELKLNTFKGLSNDSVVGKSGVE
                     LYYEKVLRGEAGEKVYKVDALGRIVPDGSRIDSLGRFVDENGEPMAVDPSKELPDRVK
                     DPVAGDDLNLTIDLDLQRVAEDELEAAIERARGAGYAATGGAVVAIDPRNGEILALAS
                     RPNFDPELFVGGISGTREMRTFQYLNSEAASYPFTDRAITGGQPAASTFKPFTGLAGL
                     TFGVIDAYTTVTDTGECWQPTGSDWGCWQSWRENSPNYAYLGPHGTQNFVEAIKDSND
                     KFFYQVADWIWQATDDQNWLP"
     gene            complement(1412..2170)
                     /locus_tag="VFT03_05320"
     CDS             complement(1412..2170)
                     /locus_tag="VFT03_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEU4847597.1"
                     /translation="MRSRCRNSEYSGRKPIARVRDMILNVMSLLLVAVGLALTATFFV
                     GSPFTNPAPAVESGALERGEPALPAIKPPSGQRAQGGLSEEQRQERREERKIVVPEDK
                     TLRVTVPAMKRVHDATIPYAGGFDEAAFKNHAGVHLRGTGSPWQKVANVYIAGHRLGY
                     MGTPSWLGFWDLNNVETGDKVFVTDSMGRRYVYRVFKEFIVDPEAVHVTQPLEGKNVL
                     TLQTCTLPDYSQRLIVQAERVEGPEPPIAQGDSS"
     gene            complement(2236..2805)
                     /locus_tag="VFT03_05325"
     CDS             complement(2236..2805)
                     /locus_tag="VFT03_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU4847598.1"
                     /translation="MRPRFVLFAALFYGLLTASAALWAALRGFDLRLVGDSLALSVAL
                     GAATAVVTVFLGLLAYRLVPALRQIAGELAPRLVDGADRGSLILISVFSGVGEETFFR
                     GAVQQEFGIVVASLLFGLVHVGPDRRFLLWTLWAVLAGFLFGGLYDLTGGLLAPILAH
                     AAHNAATLLLWKRSRKGVTERDPHEPEGS"
     gene            2898..4259
                     /locus_tag="VFT03_05330"
     CDS             2898..4259
                     /locus_tag="VFT03_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU4847599.1"
                     /translation="MIDESIFKAYDIRGVYPDSLNEDVARDIGRAFVAYLGLSGSRVI
                     VARDMRLSGEALETAFIEGVTLSGADVLDLGPVSTDALYFAVGHLEEPGGAMITASHN
                     PKEYNGLKLCREDAIALSGEHGISQIRDLIISGKLPEPAEYPGSVEESDITEDYAKHC
                     LSFINTDGLRRLKIVVDAGNGMAGKMLPPIFEKLAFIEYVPMYFELDGSFPNHPPNPI
                     EPENMKDLQERVKTEGADFGAAFDGDADRVFAVNEKGETISGDILAALVAKNVLEKEP
                     GATILYSAVCSKALPELIESEGGRAIRTKAGHSIIKPLMREHNAAFGAEHSGHYYFRD
                     NYFADSGIVAMLTVAELVARQDGPISELIAPIDNYVRSGEINLEVEDQEATLRKVEEH
                     CAQRDDPRIDHLDGLTVDYGDWWFNLRPSNTEPLLRLNVEASDRETMERERDALLDLI
                     RSS"
BASE COUNT          833 a         1430 c         1409 g          777 t
ORIGIN      
        1 tggggcaacc agttctggtc gtcggtagcc tgccagatcc agtccgccac ctggtagaag
       61 aacttgtcgt tggagtcctt gatggcctcg acgaagttct gggtgccgtg aggtccgaga
      121 tacgcgtagt tggggctgtt ctcgcgccag ctctgccagc agccccagtc cgagcctgta
      181 ggctgccagc actcgcctgt gtcggtgacg gtggtgtagg cgtctatgac gccgaaggtg
      241 agacccgcca gcccagtgaa gggcttgaag gtcgatgccg ccggctggcc gccggttatc
      301 gccctgtcgg tgaacggata gctcgcggcc tcggagttca ggtactggaa cgtccgcatc
      361 tccctggtgc ccgagatacc gccaacgaag agctctgggt cgaagttggg gcggctggcg
      421 agcgccagga tctcgccgtt gcgcgggtct atcgcgacga cggcgccccc ggtcgcggcg
      481 tagcccgcgc cccgggcgcg ctcgatggcg gcttcgagct cgtcctccgc aacccgttgc
      541 aggtcgaggt ctatggtgag gttcaggtca tcgcccgcca cggggtcctt gacgcggtcg
      601 ggcagctcct tgctgggatc gaccgccatc ggctcgccgt tctcgtccac gaaccggccc
      661 aaagagtcga tccggctacc gtcaggcacg atccggccga gcgcgtccac cttgtatacc
      721 ttctcgccgg cctcgccgcg caacaccttc tcgtagtaga gctcgacccc gctctttccg
      781 acgaccgagt cgttggagag gcccttgaag gtgtttagct tgagctcctg ctgggtgata
      841 gcgccggtgt acccgaggat gtgagcggcc atatcccctg ccgggtagtt gcggacgtag
      901 tcgtcgttaa cgacgacgcc tgggaactct tcggtgcgct ccatgacgta ggtaacgcca
      961 tcgcggtcgg cgttctcctt caccagcatg gggctatact gggtgcccca gtcgtaagct
     1021 gagtcgtagc tctcgagaac ggtcttcggg tcggccccga ggatcttggc cagctccttg
     1081 accttctcgc gcgagatgtt cgacggggtc acggtgacgt tgaggccggg gacgttgttc
     1141 gcgaggacct ggccgtcgcg gtcgcggatg acgccgcgct gggccgggtc cttgacctcg
     1201 agtgtctggg tcgcctgcgc ggcgaccgag tactcggtgc ccgtgaggat ctggaggtac
     1261 cacaaccggg cagcgaggac tatgaatacc accgcgacga cgactgcgag ggtgaccacc
     1321 ctgacttgca tgttgccctt ggtcaagggc ggcgtgtcgg gccgcttctt cgcgggccgc
     1381 gccttcttct cctcgcgcac gggaagccgg atcaactgct gtccccttgc gctatcggtg
     1441 gctccggccc ttccaccctc tcggcctgga cgatgaggcg ctgcgagtag tcgggcaggg
     1501 tgcaggtctg gagggtgaga acgttcttgc cctcgagggg ctgcgtcacg tgaacggcct
     1561 ccggatcgac gatgaattcc ttgaatactc tgtagacgta gcgccgcccc atcgagtcgg
     1621 tgacgaatac cttgtccccc gtctcgacgt tgttcaggtc ccagaagcca agccagctag
     1681 gtgtccccat atagccaagc cggtggcccg cgatgtatac gtttgccacc ttctgccagg
     1741 gtgagcccgt ccctcgcagg tggactccgg catggttctt gaaggcagcc tcgtcgaagc
     1801 cgcctgcgta ggggatcgtc gcatcgtgta cccgcttcat cgccggtacc gtgacccgga
     1861 gcgtcttgtc ctcgggaacg actatcttac gttcctctcg ccgctcctgc ctctgttcct
     1921 cagagagtcc tccctgcgct ctctgcccgg aaggcggttt gatggccggc agggcgggct
     1981 cacctctttc gagtgccccg ctctccacgg cgggggcagg gttggtgaat ggggagccca
     2041 cgaagaaggt cgccgtcagg gcgagcccca ccgctaccag gagcaggctc atcacgttca
     2101 agatcatgtc cctgacgcgg gctatgggct tcctccccga gtactctgag tttctacacc
     2161 tagatcgcaa cccattgtat acaaaaagcc ggcgactact aggccttgtc gcgaatgtag
     2221 tttttcaccg ccggactacg agccttcagg ctcgtgtgga tcacgctccg tcacgccctt
     2281 tctcgaccgc ttccagagca agagcgtcgc ggcgttgtgc gcggcgtggg cgaggatcgg
     2341 ggctagaagc ccgccggtga ggtcgtagag gcctccgaac aggaagccgg ccaggactgc
     2401 ccagagggtc cagagcagga agcgcctgtc gggcccgaca tgcacgagcc cgaaaaggag
     2461 agaggccacc acgataccga actcctgttg cacagcgccc cggaaaaacg tctcctcccc
     2521 cacccccgag aagacggaga tgaggatcag gcttccacga tctgcgccgt ccacgaggcg
     2581 cggggcgagt tccccggcta tctgccgcag cgcgggcacg aggcggtaag cgagtagccc
     2641 caggaatacc gtgacgacag cggttgcggc acccagagcc acgctcaggg cgagcgagtc
     2701 tcccacgagc ctaaggtcga acccccgcag ggccgcccag agcgccgccg aagcagtaag
     2761 gagcccgtag aagagggccg cgaaaaggac gaatcgtggt ctcaccgggc gctatccggc
     2821 ctggtctgca cgaggggcca cgagaagtta ttgtatcgga ggcgaaagac gggcgatccg
     2881 agaggtagaa ggcgaagatg atcgacgagt cgatattcaa ggcctacgac atccgcggcg
     2941 tgtaccccga cagcctgaac gaggacgtgg cccgagacat cggccgcgcc ttcgtcgcat
     3001 acctcggcct ctccggctcg agggtcatcg tcgcccgcga catgcgcctg agcggcgagg
     3061 ctctcgagac ggcgttcata gagggcgtca ccctgtcagg cgctgacgtc ctcgacctcg
     3121 ggcccgtctc gacagacgcg ctctacttcg ctgtcgggca cctggaggaa ccgggcggag
     3181 ccatgatcac ggcttcccac aaccccaagg agtacaacgg cttgaagctc tgcagggagg
     3241 acgccatagc cctctccggg gagcacggca tcagccaaat acgggacctc atcatctcgg
     3301 gcaagctgcc cgagccagcc gagtatccag gctccgtgga ggagagcgac atcacggagg
     3361 actacgcaaa gcactgcctg agcttcatca acaccgatgg gctgcgccgg ctcaaaatcg
     3421 tggtggacgc aggaaacggg atggcaggca agatgctccc tcccatcttc gagaaactgg
     3481 cgttcatcga atatgtcccc atgtacttcg agctcgacgg ttccttcccc aaccatccgc
     3541 ccaacccgat cgagccggag aacatgaagg acctgcagga gcgcgtaaag accgaaggag
     3601 cggacttcgg cgccgccttc gacggggacg ccgacagggt cttcgcggta aacgaaaaag
     3661 gcgagacgat ctccggcgac attctcgccg cgctcgtcgc aaagaacgta ctcgaaaagg
     3721 agccaggcgc caccatcctc tacagcgctg tgtgttccaa ggctctcccc gaactcatcg
     3781 agagcgaggg cggaagggcg atccggacga aagccggaca ctccatcatc aaacccctga
     3841 tgcgcgagca caacgccgct ttcggcgccg agcactcggg gcactactac ttccgggaca
     3901 actacttcgc cgactccggc atagtcgcca tgctcacggt cgccgagctc gtcgcgcgcc
     3961 aggacggccc gatctccgag ctgatagcac ccatagacaa ctacgtccgg tcaggggaga
     4021 tcaacctcga ggtcgaggac caggaggcga cgctgcgaaa ggtcgaggaa cactgcgcgc
     4081 agcgcgacga tcccaggata gaccacctcg acggcctcac cgtcgactac ggagactggt
     4141 ggttcaacct ccgcccctcg aacaccgagc ccctgctgcg gctcaacgtc gaggctagcg
     4201 accgcgagac catggagcgc gagcgcgacg cgctgctaga cctcataagg agtagttagc
     4261 caacactcca tatttgaacc tgtatagaaa accccgcgca agaagaagcg cccctgcgaa
     4321 tggcgggggc gccacttaag tggttggttg aaaccgacgg acgaataggc tgagaggcta
     4381 ccgaaccgcc gggcgacgcc tgaggcggcg tgctgactac tcgccagcta ttatcaggag
     4441 gttcctcac
//
LOCUS       DASTUV010000265         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1077045, whole genome shotgun sequence.
ACCESSION   DASTUV010000265 DASTUV010000000
VERSION     DASTUV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077045"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VFT03_05335"
     CDS             complement(<1..138)
                     /locus_tag="VFT03_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4847600.1"
                     /translation="MPLLEIEDMHSYYGHIEALRGISLTVDEGEVVTLIGSNGAGKTT
                     TL"
     gene            complement(145..921)
                     /locus_tag="VFT03_05340"
     CDS             complement(145..921)
                     /locus_tag="VFT03_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4847601.1"
                     /translation="MNDENILEARGITKVFGGLIAVSEVDFDIPERSIVSLIGPNGAG
                     KTTFFNMVSGLYEPSAGTFVFQGRDITKYRAHKRAQMGIGRTFQNIRLFDTMSAGENV
                     LAGMHTRLKGRILGSIFSTPGTKREEREGRERAAELLDFVGISERETFAKNLSYGDQR
                     RLEIARALASEPRLLLLDEPTAGMNPQETARLTDLIGRLREERGIAILLIEHEMRVVM
                     SISDRVTVLDHGEKISEGTPREVRDDPRVIEAYLGTAKTG"
     gene            complement(914..2086)
                     /locus_tag="VFT03_05345"
     CDS             complement(914..2086)
                     /locus_tag="VFT03_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU4847602.1"
                     /translation="MALLRKYGLPALLIVAAFVFPIVDSTFELDLIFPVIIIAIYILL
                     ALGLNIVVGFAGLLDLGFVAFYALGAYACGWLASSQFKQLSFSLGSTATSLTGEALPG
                     IHISFWIILFVAAAFAGLCGVIIGAPTLRLRGDYLAIVTLGFGEIIPRFFQNADDLGG
                     FNLTNGTIGIKAIDSPGLQFLPDTLDAWQRFGTLYLHPWYYTILILVLITVYVNVRLR
                     DSRLGRAWISVREDETAAAAMGINPVTTKLWAYALGAVFGGIAGAFYGAFIKNIFPDA
                     FSFNISILVLCMVILGGMGNIYGVILGAVILQGLNFYLLPQINVWVHAIGDAVGSPAL
                     SQADIPKYNFLLFGLLLVLMMLLRPEGIIPNRQRAEELHSGEGGDVGDALGGSPRE"
     gene            complement(2091..3179)
                     /locus_tag="VFT03_05350"
     CDS             complement(2091..3179)
                     /locus_tag="VFT03_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU4847603.1"
                     /translation="MTTTTEATKSRSDELLRSIRSSSWGSRIAVVLISALVVVLAVQA
                     AREPAQFASQLVIGVTNGAIIALVALGYTLVYGIIELINFAHGDNFMIGSFVGLTVLS
                     GTVLGIGLFVPITDDSGTLLRLAGVLIAMAVAMIACGLLGVGIERVAYKPLRNSPRLA
                     VLITAIGMSFILQNVGLIWKGPAQIPFPELISNANILPTDAIIFRWKDLFVIGVTIPL
                     LVALSYFISNTKQGKAMRAVAQDKEAAAMVGINVNRTISVCFLLGGILAGASGVMYGL
                     FNGITVFNVGFQQGLYAFTAAVLGGIGNLTGAVVGGFLIGIIGSMADQYIGVRWTAVV
                     IFAVLIMVLVFRPTGLLGDRSAVQGGGE"
     gene            complement(3243..>4347)
                     /locus_tag="VFT03_05355"
     CDS             complement(3243..>4347)
                     /locus_tag="VFT03_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4847604.1"
                     /translation="QTTTMNNAIKLALQERDGKAGDINIEFEEMDDATAQAGQWDAAK
                     CAENAQSAAQDEEVIGWIGPFNSGCAAVEIPINNEAGLAMISPANTYIGLTKEGGESD
                     EPEKYYPTGERNYARVIVADDKQGAAGAKLMEEEGMESVFILDDKETYGKGLADQFQR
                     AAEDSGIEVVGREGIDGSAPNYRSLMNKIATENPDAIYFGGIIENNAAQLIKDKVGAG
                     MSNDEVAFIGPDGIFVDTLLSQAGDSAEGIWVTFGGLPESELSQKGKEFVDKYESAYD
                     DAVQPYTVYAYEAANVMLDAIERASKEAGGEVPDREAVVKQVFATEDYNGALGTWSFD
                     EDGDTTLTELSIQRVENGEFVYQQTVDVRDLVQ"
BASE COUNT          772 a         1404 c         1369 g          802 t
ORIGIN      
        1 cagagtcgtg gtcttgccgg ccccgttcga accgatgagg gtaacgacct cgccttcgtc
       61 tacggtgagc gagatcccgc gcagagcctc gatgtgcccg tagtagctgt gcatgtcctc
      121 tatctcgagc agcggcatag aggcttaccc tgtcttcgcc gtgccgaggt aggcttcgat
      181 gacgcgcggg tcgtcgcgca cctcgcgcgg cgtgccctcg gagatcttct ccccgtggtc
      241 gagcaccgtg acccggtcgg agatgctcat gaccaccctc atctcgtgct cgatgaggag
      301 gatggcgata cccctctctt ccctcaggcg cccgatgagg tccgtcaggc gcgccgtctc
      361 ctgcgggttc attcccgcag tcggctcgtc caggaggagg agcctgggct ccgaagccag
      421 ggcgcgcgct atctccaggc gccgctggtc cccgtaagag aggttcttgg cgaacgtctc
      481 gcgctcgctg atgccgacga agtcgagcag ctccgcggcc ctctcgcgcc cctcccgctc
      541 ctcgcgcttg gtccccggcg tgctaaagat cgagcccagg attctgcctt taagacgcgt
      601 gtgcatcccg gcgagcacgt tctccccggc gctcatggtg tcgaagagcc ggatgttctg
      661 gaaggtgcgc ccgatgccca tctgggcgcg tttgtgcgcc cggtacttcg ttatgtcgcg
      721 gccctggaac acgaaggtcc cggcagaggg ctcgtagagc ccgctcacca tgttgaagaa
      781 cgtcgtcttg cccgcgccgt tcggcccgat caggcttact atcgagcgct cggggatgtc
      841 gaagtccacc tcgctgaccg cgatgagccc gccgaagacc ttggtgatcc cacgcgcctc
      901 gaggatgttc tcatcattca cggggcgagc cccccagcgc gtcgccgacg tcgccgcctt
      961 cgccgctatg caactcctcg gcgcgctggc ggttgggaat gatgccttcg ggccgcagga
     1021 gcatcatgag gacaaggagg aggccgaaca gcaagaagtt gtacttcggg atgtccgcct
     1081 ggctgagcgc ggggctccct acggcgtcgc caatggcgtg gacccacacg ttgatctgcg
     1141 gcagcaggta gaagttgagc ccctgcagga tgacggcgcc gaggatcacg ccgtagatgt
     1201 tccccatgcc accgaggatg accatacaca ggaccagtat cgagatgttg aacgagaagg
     1261 cgtcggggaa gatgttcttt atgaacgcgc cgtagaaggc gcccgcgatg ccgccgaaga
     1321 ccgcgccaag ggcgtaagcc cagagcttcg tcgtaaccgg gttgatgccc atcgcggcgg
     1381 ctgcggtctc gtcctcgcgg accgagatcc aggcccgccc gagcctcgag tccctgaggc
     1441 gcacgttgac gtagaccgtg atcaggacca gaatcagtat cgtgtagtac caggggtgca
     1501 ggtacagagt cccgaagcgt tgccaggcgt ctaaggtatc aggcaagaac tgcaaccccg
     1561 gcgagtctat ggccttgatg ccaatggtgc cgtttgtcag gttgaagccg cccaggtcgt
     1621 ccgcgttctg gaaaaagcgt gggatgatct ctccgaaccc cagggtcact atcgcgaggt
     1681 agtcgccccg caacctcagg gtaggcgcgc cgatgatcac gccgcacagg ccggcgaagg
     1741 ccgcggctac gaagaggatg atccagaacg agatgtgtat cccaggcaac gcctcgccgg
     1801 taaggctcgt cgcggtggac cccaacgaga agctcaactg cttgaattgg ctcgaggcga
     1861 gccagccgca agcgtaggct cccagggcgt agaacgccac gaagccgagg tcgagcaggc
     1921 cggcgaatcc gacgacgatg ttcaggccga gcgcaagcaa gatatagatg gcgatgatga
     1981 tgacggggaa gatcagatcg agctcgaaag tgctgtcgac gatcgggaag acgaaagccg
     2041 ccacgatgag cagcgcgggc agcccgtact tcctcagcag ggccacgttg ctactccccg
     2101 cccccctgca cagccgagcg gtccccgagc aggcccgtag gccggaacac gagcaccatt
     2161 atcaggacgg cgaagatcac gaccgcggtc catctgacgc ctatgtactg gtcggccatg
     2221 gacccgatga tgccgatgag gaaccccccg accacggccc cggtcaggtt gcctatgccc
     2281 ccaaggaccg ctgccgtgaa cgcgtagagt ccctgctgga accccacgtt gaagaccgtg
     2341 atgccgttga agagcccgta catcacgcca gaggccccgg cgaggatgcc gccgaggagg
     2401 aaacagacgg agatagtgcg gttcacgttg atgccgacca tcgctgccgc ctccttgtcc
     2461 tgcgccacgg cccgcatcgc ctttccctgc ttggtgttgc ttatgaagta ggaaagtgcg
     2521 acgagcagcg gaatcgtcac cccgatcaca aagaggtcct tccagcggaa gatgatcgcg
     2581 tccgtcggca ggatgttggc gttggagatg agctcgggga acgggatctg ggcggggcct
     2641 ttccagatga ggcccacgtt ctggaggata aacgacattc cgatcgccgt tataaggacc
     2701 gccagccgcg gcgaattcct caaaggcttg tacgcgacgc gttcgatccc cacgccgagc
     2761 agcccgcagg cgatcatggc cacggccatg gctatgagga ccccggccag cctgagcagg
     2821 gttccagagt cgtctgttat cggaacgaag agacctatcc ccaggaccgt tccgctaaga
     2881 acggtcagcc ccacgaagga gccgatcatg aagttgtcgc cgtgggcgaa gttgatgagc
     2941 tcgatgatcc cgtagaccag cgtgtagccg agggctacga gcgcgatgat ggccccgttg
     3001 gtcacaccga tgacgagctg cgaggcgaac tgcgccggct ccctagccgc ctgcacggca
     3061 agcaccacca cgagcgcgga gatgaggacc acggcgatgc gcgaccccca gctcgaggac
     3121 ctgatgctcc gtaggagttc atcggagcga gacttcgtgg cctcagtggt ggtggtcatt
     3181 ccacaccctc tttcgaaaaa ggggaccggc catcatcatg ccggtcccct ggctccgtgc
     3241 tcctactgga cgagatcccg gacgtcgacg gtctgctggt acacgaactc accgttctcg
     3301 acccgctgga tggagagctc cgtgagcgtt gtgtcgccgt cctcgtcgaa gctccaggtg
     3361 ccgagggcac cgttgtagtc ctcggtagcg aagacctgct tgaccaccgc ctcgcgatcg
     3421 ggcacctcgc cgccggcctc cttggaggcc cgctcgatgg cgtcgagcat cacgttagcc
     3481 gcctcgtagg cgtagacggt gtaaggttgg accgcgtcgt cgtacgcgct ctcgtacttg
     3541 tcgacgaact ccttgccttt ttgggagagt tcgctctccg gcagcccgcc gaaggtgacc
     3601 cagatgccct ctgcggagtc gcccgcctgg ctgaggagcg tatccacgaa gatcccgtcg
     3661 ggcccgataa aggcgacctc gtcgttgctc atgccggcgc cgaccttgtc cttgatgagc
     3721 tgagccgcgt tgttctctat tatgccgccg aagtagatgg cgtcggggtt ctccgtcgcg
     3781 atcttgttca tcagcgaacg gtagttggga gcggagccgt cgatgccctc gcgccccaca
     3841 acctctatgc cgctgtcctc cgcagcccgc tggaactggt cggcgaggcc cttgccgtag
     3901 gtctccttgt cgtcgaggat gaagacggac tccatgccct cttcctccat cagcttcgcg
     3961 cccgcggcgc cctgcttgtc gtcggccacg atgacgcgcg cgtagttgcg ctcgcccgtc
     4021 gggtagtact tctccggctc gtccgactca ccgccctcct tggtcagacc gatgtacgtg
     4081 ttggccgggc tgatcatggc gagtcccgcc tcattgttga tggggatctc caccgcggcg
     4141 cacccggagt tgaacggccc gatccagccg atgacctcct cgtcctgcgc ggccgactgg
     4201 gcgttctcgg cgcacttggc cgcgtcccac tggcctgcct gcgccgtcgc gtcatccatc
     4261 tcctcgaact cgatgttgat gtcgccagcc ttgccgtccc gctcttgcag cgccagcttg
     4321 atggcgttgt tcatagtagt ggtctgg
//
LOCUS       DASTUV010000266         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_443810, whole genome shotgun sequence.
ACCESSION   DASTUV010000266 DASTUV010000000
VERSION     DASTUV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443810"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..429)
                     /locus_tag="VFT03_05360"
     CDS             complement(139..429)
                     /locus_tag="VFT03_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847605.1"
                     /translation="MRRSAGSTIDPGVFWVSLGISMLFVVWGVFFTDNLSAIIFAAPF
                     VLVMICMCYSLFKELREERVARPAPEPERAAGRMTGAPAPQQSSSLEREAGG"
     gene            complement(707..1978)
                     /locus_tag="VFT03_05365"
     CDS             complement(707..1978)
                     /locus_tag="VFT03_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU4847606.1"
                     /translation="MADVADGRPAEAPRTLGSRLRLVGPGIVIAVTGVGAGDMVSSLV
                     AGTDFGMVLIWAVVLGALLKWFLTEGIGRWYMASGQTILQGWHSLGRGATYYFIVYLF
                     IVTFVFGAAVTSTAALAVDAAFPDTLPLWLWAALHGVAAFVIVGIGRYGLFELIMKTF
                     AGLKFGIVILLAILLAPNLGELALGFVPRIPEGALINVLAIIGGVGGTYSLAAYNYWV
                     RERGWRQSSWIPMMRTDVTVGYALTALFMVSMLVIGAELLFGTGTSISDDEGLVALVN
                     PLQDRFGVLASWAFLIGFWAVATGAMIGAWNGGAYLFADTIRTIREVPDDEAEEYLSE
                     KSLYFRGFLAWMTFPPMVLLALGQPVALVIVYASLGALFLPFLAITLLLLLKSNRVAP
                     EYRNRIVSKVFLVASVIPFVVVGVQEVLGSL"
     gene            complement(2068..>2475)
                     /locus_tag="VFT03_05370"
     CDS             complement(2068..>2475)
                     /locus_tag="VFT03_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847607.1"
                     /translation="VAQGLTLGPLIRALRLKVEGSQGTLMELRARLEGARAALERLER
                     LCDDERVPQGAQEQMREHYEGRIRRYEDGIEAGGVTEEYSESSAAWRNWRRDLIGAER
                     EAILSLRDSGDISPEVMRRIERDLDLEESRIGG"
BASE COUNT          489 a          830 c          757 g          399 t
ORIGIN      
        1 aggtccgccc tccggtcgag ctcctcgtag gtgagccggg ttcgcgtatc ctctatggct
       61 ggcgccgacg gggcacgccg cgcctgttcc tcgaagagct cgtgcaggca cagatccgtc
      121 tgtcgtgtgc tcgcttcgct atccaccggc ttcgcgctcc aggctgctgc tctgctgggg
      181 cgcgggcgct cccgtcatgc ggcccgccgc gcgctccggc tccggcgcgg gacgagcaac
      241 cctctcctcg cgcagctctt taaagagcga gtagcacatg cagatcatga ccagtacgaa
      301 cggcgcagcg aagatgatgg ccgagaggtt gtccgtgaag aatacgcccc acaccacgaa
      361 gagcatggag atgcctaaag agacccagaa cacccccgga tctatcgtag atcccgcaga
      421 cctgcgcaaa gttgctcacc ttccccttac gttcgctcaa agtaatccag cactacgtaa
      481 accgtaatcg gtgcgacaga acgtcgcggt agaagcgttg caggccacgc tcaggtcgag
      541 gaccgggagc gaagcagccc ggaaacgttg atgaacatac tacccgcacc cggcgcccgc
      601 ggaacctgtt atttcgaaga ttcctttaag acggtgttaa atttcgttaa gagatggggt
      661 gcgggggtgg ggggcacgcg gacgacgtgc tccccgaggt cctatctcag aggctaccca
      721 gaacctcctg cacgccgacg acgacaaacg ggatgaccga ggctacgagg aagactttag
      781 atacgatccg gttacgatac tcgggagcga cgcggttgga tttcaggagc agaagcagtg
      841 tgatcgcgag gaacggcagg aacagggcgc cgagcgaggc gtagacgatc accagcgcca
      901 ccggctgtcc taaggcgagc aggaccatcg gcgggaaggt catccaggcc aggaaacccc
      961 ggaagtacag gctcttctcc gagaggtact cttctgcctc gtcgtcgggc acctcgcgga
     1021 tggtccggat ggtatcggcg aagaggtagg cgccgccgtt ccaggccccg atcatggccc
     1081 cggtagcgac ggcccagaag ccgatgagga atgcccagct cgccaggacg ccgaagcggt
     1141 cttgcagggg attgaccagc gccaccaggc cctcgtcgtc gctgatcgag gtacccgtgc
     1201 cgaagagcag ctcggcaccg atgaccagca tcgacaccat aaacagggcc gtcagcgcgt
     1261 agcctaccgt cacgtcggtc cgcatcattg ggatccagga cgattgccgc cagccgcgct
     1321 cacgcaccca gtagttgtaa gcagccaggg agtacgtccc gcccacgccg ccgatgatgg
     1381 cgaggacgtt gatcagcgcg ccctccggta tccgcgggac gaacccgagg gcaagctcgc
     1441 caaggttcgg ggccaacagg atcgccagca gtatgacaat accgaacttc aggccggcga
     1501 aggtcttcat gatcagctcg aagagcccgt agcgtcctat gccgacgatc acgaaggccg
     1561 caacgccgtg cagagcggcc cacagccaca gtggcagcgt gtcggggaag gcggcgtcaa
     1621 cagccagcgc ggcggtcgag gtcaccgccg ccccgaagac gaaggtcacg atgaacaggt
     1681 aaacgatgaa gtagtaggtc gctccacgtc cgagcgagtg ccagccctgc aagatggtct
     1741 gacccgaggc catgtaccag cgccctatcc cttctgtcag gaaccacttc aggagcgcgc
     1801 cgagcacgac ggcccagatc agaaccatcc cgaagtccgt cccggccacc agggacgaga
     1861 ccatgtcccc ggcgcccacg ccggtcaccg cgatgactat gccaggcccg acgagtctca
     1921 gccggctacc taaggtcctc ggagcctccg ccgggcgtcc atctgccacg tcagccatca
     1981 gtcccctccc cactctcgcc ttccctcgat acattagaca tcattcatgt ctcggacaca
     2041 atggtccgac caggggaatt ctgtatgtca gccgccgatc cgggactcct cgaggtccag
     2101 gtcgcgctcg atgcggcgca tgacctcggg gctgatatcg ccgctatcac gcaacgaaag
     2161 gatggcctcc ctctcggccc cgatcaggtc tcgccgccag ttgcgccagg ccgcggagct
     2221 ctccgagtac tcctctgtga cgccgcccgc ctcgatgccg tcctcgtagc gccggatgcg
     2281 gccttcgtag tgctcgcgca tctgctcctg ggcaccctgt gggaccctct cgtcgtcgca
     2341 gaggcgctcc agccgctcga gggcggcgcg ggctccttcg aggcgggccc tgagctccat
     2401 gagcgtaccc tggctccctt cgaccttcag gcgcaacgcc ctgatgagcg gtcccagcgt
     2461 cagcccttgc gcgac
//
LOCUS       DASTUV010000267         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_55074, whole genome shotgun sequence.
ACCESSION   DASTUV010000267 DASTUV010000000
VERSION     DASTUV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55074"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFT03_05375"
     CDS             complement(<1..352)
                     /locus_tag="VFT03_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847608.1"
                     /translation="MSLGGGGEPRREPGETPGASSPERGAWREGRFRLSPLLLLFAAL
                     LLALLAVGGLLLWRSLGDNLSGTSVARDSIDSGPEPRVRLTNAAGQVRVEGVEGLDSV
                     EYEVTKYAMGPDPAA"
     gene            complement(381..743)
                     /locus_tag="VFT03_05380"
     CDS             complement(381..743)
                     /locus_tag="VFT03_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEU4847609.1"
                     /translation="MDQRPPSVKVERPWGNFEQYTHNLTSTVKVITVVPGGVLSRQYH
                     HKRDELWVVLDAGACVELDGEVLHPEPGEKLFIPRETVHRLSSVGEREARVLEISFGE
                     FDEDDIVRIDDVYGRAGP"
     gene            860..1225
                     /locus_tag="VFT03_05385"
     CDS             860..1225
                     /locus_tag="VFT03_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4847610.1"
                     /translation="MPRLEVEGAGTFDVEDGKRLVLAIEEDASVDILHRCGSYARCTT
                     CRIEYLEGEPEKMTKAEVEVLESRDLLGQVRLSCQALCDHDMKVRVLMTVSSTGLDGP
                     GPKPERHITPEPEWVDKPY"
     gene            complement(1427..2392)
                     /locus_tag="VFT03_05390"
     CDS             complement(1427..2392)
                     /locus_tag="VFT03_05390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU4847611.1"
                     /translation="MAVDFGVFVPQGWRMDLVEIEDPFEQYEAMTRVAKVAEGTGAYD
                     SIWVYDHFHTVPEPTRETTFECWTITAGLSRDTQEIKIGQMVTCNGYRNPALLAKISS
                     TVDVMSNGRLLFGLGAGWYEHEWRAYGYGFPEVPVRMRMFKEGCEIVHRMWTEDDVVF
                     KGEFYQIDKPINEPRSLQSPHPPFWIGGSGEKVTLRLVARWGDACNVNGDPERLRHKF
                     GVLRGHCEDLGRDYEEITRSTSINVFLLEEGDDPVEATTLARGEISYDKYSNQFMVGT
                     AEEIVERLRPKVEAGVNYFITYMPRVAYEPEMVERFAREVIPELS"
     gene            complement(2472..2996)
                     /locus_tag="VFT03_05395"
     CDS             complement(2472..2996)
                     /locus_tag="VFT03_05395"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU4847612.1"
                     /translation="MGERNPPVSTGRGDDGTTTLLGSGRMGKNAPRIIVLGDLDEASS
                     FLGLARAEADGDIGELLLRLQRLLYRVMGDVAMPKEPNAVGEEDLRFVDEALEEWRGK
                     TEISREFVVPGESRLCALLDVARTVVRRSERSLVAAGYETEHPYALRAVNRLSDLLFV
                     VARNADGRRTLSKD"
BASE COUNT          521 a         1044 c         1021 g          586 t
ORIGIN      
        1 ccgcggccgg gtcggggccc atagcgtatt tagtcacctc atactcgacg gagtcgagcc
       61 cctcgacgcc ctccaccctc acctgtcccg cggcgttggt gaggcgcacg cgcggctctg
      121 gccctgagtc tatggagtcc cgcgcgacgc tggtgcccga gaggttatcg cccagggacc
      181 tccacaggag caggcccccc accgcgagga gcgccaggag cagagcggcg aagagcagga
      241 gcagggggga gaggcggaac cggccttctc gccacgcacc cctctccggg gaagaggcgc
      301 cgggcgtctc ccccggctcg cggcgcggct cccctccacc cccgaggctc acccggagag
      361 cgcgggacgc ggagggtcca tcacggcccc gcgcggccgt agacgtcgtc gatgcggact
      421 atgtcgtcct cgtcgaactc gccgaaggag atctcgagca cccgcgcctc cctctctccg
      481 accgaagaga gcctgtgcac cgtctccctg gggatgaaga gcttctcacc cggttcgggg
      541 tggagtacct ctccgtccag ctcgacgcaa gcgcccgcgt cgagcaccac ccacagctcg
      601 tcgcgcttgt ggtggtactg gcgcgacagg acgccgccgg ggacgaccgt tatgaccttg
      661 accgtggagg tcaaattgtg ggtgtactgc tcgaagttgc cccagggcct ctctaccttg
      721 accgaaggcg gcctttgatc caatcccaac cccctaactc gtgcgcgggg gaagtttaga
      781 ctatctgttc cctgttgtgg agagcccccc ttgtggcaat ctgctacgct cttgacgata
      841 tccaagagag gagggaccga tgccgaggct tgaggtcgag ggcgcgggga ctttcgacgt
      901 agaagatggt aagaggctgg tgctcgcgat cgaggaggac gctagtgtgg acatcctgca
      961 ccgctgcggc tcgtatgcaa ggtgcaccac ctgccgcatc gagtacctcg aaggcgagcc
     1021 cgagaagatg acgaaggcgg aggtcgaggt gctcgagagc cgggatctgc tcgggcaggt
     1081 caggctctcc tgccaggctt tgtgcgacca cgacatgaag gtgcgcgtcc tgatgaccgt
     1141 gagctctact ggcctcgacg gcccgggacc gaagccggag cgccacatca cacccgagcc
     1201 cgagtgggtg gacaaaccgt actgaatcgc tatgggtagt ggctcgcccc tcccaatagc
     1261 gctttagtgc tccggggcct tcccgaaggc tttcgtccgg acctcggcct gcccggcgaa
     1321 ggggaagcgt gagggcgtct actacttcct cgccgccctc acgcttgatc tcgctttcag
     1381 gcgcgcacga ggtacctctc gaggtgccgc ccggacaagc ctcctctcag gaaagctcgg
     1441 ggatgacctc cctggcgaag cgctcgacca tctcgggctc gtaggcgacg cgcggcatgt
     1501 aggtgatgaa gtagttgacc cccgcctcga ccttcgggcg cagccgctcg actatctcct
     1561 ccgccgtgcc gaccatgaac tggttcgagt acttgtcgta gctaatctcg ccgcgggcga
     1621 gggtcgtggc ctccacgggg tcgtctccct cttcgagcag gaagacgttt atggaggtgg
     1681 agcgtgtgat ctcctcgtag tcgcgcccca ggtcctcgca gtgccctcgc agcacgccga
     1741 acttgtgcct tagcctctcg gggtcgccgt tgacgttgca ggcgtcaccc cagcgggcga
     1801 cgagcctcaa cgtaaccttc tcgccgctgc ctccgatcca gaacggaggg tggggcgact
     1861 gtaagctcct gggctcgttt atgggtttgt ctatctggta gaactcgcct ttgaagacga
     1921 cgtcgtcctc ggtccacatg cggtggacga tctcgcaacc ttccttgaac atccgcatcc
     1981 gcacggggac ctcggggaag ccgtagccgt aggcgcgcca ctcgtgctcg taccagccgg
     2041 cgcccaggcc gaagaggagc cgcccgttgc tcatcacgtc tacggttgag gaaatcttgg
     2101 ccaggagcgc ggggttgcgg tagccgttgc aggtgaccat ctgtccgatc ttgatctctt
     2161 gcgtgtcgcg gctcaggccg gctgtgatcg tccagcactc gaaggtggtc tcacgggtag
     2221 gctccgggac ggtgtggaag tggtcgtaga cccagataga atcgtaggcg ccggtcccct
     2281 cggcgacctt cgccacccgt gtcatcgctt cgtactgctc aaaggggtct tctatctcga
     2341 ccaggtccat ccgccacccc tgcgggacga agacgccgaa gtcaaccgcc attgactacc
     2401 tccttggagg gatcgtgctc gctgtgcgga tctatacccg gatttgcaac ccgcaatcga
     2461 agtaccggcg ttcagtcctt cgagagcgtc ctccgcccgt cggcgttgcg ggcgactacg
     2521 aagagcaggt cagatagccg gtttacggct ctgagggcgt atgggtgctc cgtttcgtag
     2581 ccggctgcga ccaggctccg ctccgagcgc ctgacgacgg tgcgggcgac gtcgaggaga
     2641 gcgcagagcc ggctctcgcc ggggacgacg aactccctgg agatctccgt tttcccccgc
     2701 cactcctcga gggcttcgtc cacgaacctc agatcctcct ctcccacggc gttcggctcc
     2761 ttgggcatcg ccacgtcgcc catgacgcgg tagaggagtc gctgcagccg gagcagtagt
     2821 tcgccgatgt cgccatcggc ttcggcacgg gcgaggccga ggaaggagct cgcctcgtcc
     2881 agatctccga gcacgatgat gcgcggggcg ttcttgccca tccggcccga ccctagcagg
     2941 gtcgtggtcc cgtcgtcgcc gcggccggtc gagaccggcg ggttgcgctc gcccatcgtg
     3001 gtctcctcgt ctcaaagata gagaggtctc cggcacggta gcgcggaagg ggcgtcctcg
     3061 caagcgtccg atgaaggtga gacgtgcctc ctggctacca catcaggctg gatgccgagc
     3121 tcttcacgct ccgtcgagaa gatgatgatc ctatagttgc cccttcgcag cg
//
LOCUS       DASTUV010000268         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1467171, whole genome shotgun sequence.
ACCESSION   DASTUV010000268 DASTUV010000000
VERSION     DASTUV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467171"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFT03_05400"
     CDS             <1..982
                     /locus_tag="VFT03_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847613.1"
                     /translation="KRMLGRLSDRGYRTLAAYEAEFYVWDAEGPLDRTPYAGSYALTP
                     AADFVAELAQTLEEMGIRPEQCHAEVGQGNLELSVGEDEALAAADKRVMVLEAIRGVA
                     HRMGLGTTMAPKPYLEGAGNGHHLHISLYEDEAPVLFDASGALSGPGLGFVAGILEHL
                     PAVMAFTAPSPNSYQRLAPGMWASAYAAYGLDNREAAVRLASPVAGRESATANVELKP
                     VDVTSNPYLALAAVLAAGMDGIDRGLDPGEPTMVDPATLSDEERASRGILPLPASPDE
                     ALDALEEDHALIEALGEPLVHTHVAVARAQAKIARELPPEEVASTAAMLY"
     gene            1140..2279
                     /locus_tag="VFT03_05405"
     CDS             1140..2279
                     /locus_tag="VFT03_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4847614.1"
                     /translation="MQTVDLTQIPVVDNHCHGILRDQTFDDLASWRQSFTESTEPQMA
                     RDHVASTTFYRRLIRTLADFLGCEPEEEVVFAARTGRDGRELTGALLRAANVDTLLID
                     TGFPPPEEVFPVPELGDLGGCRVEPMLRLEILMESLLAEHDSLEDVKEALAAELEDAR
                     GRGYVALKSIAAYRTGLDVREWPSEEAEESFLEYRRADGPGPARLVHKPLLDTLLHVA
                     FAQAARQEIPLQFHVGYGDADTDLLLGNPLYLRPVLQRPDYRGMPVVLLHECYPYTRQ
                     GGYLAAVYQNVYLDLSYGIPLLGYGEMLSFTRQAFGVAPSSKLLYSSDGIGVPELHWI
                     SAIDGRRVIGEALGELVSRGELDLAAAEAAGEDVLRANATRLYGL"
     gene            2384..>2670
                     /locus_tag="VFT03_05410"
     CDS             2384..>2670
                     /locus_tag="VFT03_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847615.1"
                     /translation="MYRQTRVLGVLLILQVGAMVGIGVWLFASIDWDTIVNLSNKPGQ
                     IRLQATDKAEVKDLKRAILFAVYFFAPVVLLLLAGLGFVLLRRRGWMLASVA"
BASE COUNT          481 a          835 c          884 g          470 t
ORIGIN      
        1 caagcggatg cttggacggc tttcggatag agggtacagg acgctcgccg cctacgaggc
       61 cgagttctac gtctgggatg ccgagggtcc gctggacagg acgccttatg cggggagcta
      121 cgcgctgacc ccagcggcgg acttcgtcgc ggagctggca cagacgctgg aggagatggg
      181 tatccggccg gagcagtgcc acgccgaggt cgggcaggga aacctcgagc tctccgtcgg
      241 ggaggacgaa gcgttagctg cggcggacaa gcgcgtgatg gtacttgagg cgatcagggg
      301 tgtggcccac cggatgggac tcgggaccac gatggccccg aagccgtatc tcgaaggagc
      361 aggcaacggc caccacttgc acatcagcct ctacgaagac gaggccccgg tcctcttcga
      421 tgcctcagga gccctgagtg gccctggctt ggggttcgtg gccggtatac tggagcatct
      481 tcccgccgtc atggcgttca ccgccccgag ccccaactcc tatcagcgcc tcgctcctgg
      541 catgtgggcc tcggcttacg ccgcctacgg cctggataac agggaggcag ccgtgcggct
      601 cgcctccccc gtggccggca gagagtccgc gaccgcaaac gtcgagctca agccggtcga
      661 cgtcacgtcg aacccctacc tggccctggc cgcggtcctg gccgccggga tggacggtat
      721 tgatcgcggc ctcgaccccg gggagccgac gatggtggac ccggccacgc tcagcgacga
      781 ggagcgcgcc tccaggggta tcctgccact ccccgcatcc ccggacgagg ccctggacgc
      841 gctggaagaa gatcatgccc tcatagaggc gctgggtgaa ccactcgtgc acacccacgt
      901 ggccgtggcc cgggcgcagg ccaaaatagc aagagagctt ccaccggaag aagtagcgtc
      961 cacagccgcg atgctctact agaagacagg ttacaggtat tttgccgata cctgtaacct
     1021 ccggtctgat gcctgcaaga aaggaagcgt gatcatatgg ccgccctcgc ggccatgtta
     1081 cgggccgcag gacgaatcga aggatctcta ccacgtgatg ctttgaacgg aggtggcgta
     1141 tgcaaactgt agacctcacc cagatccccg tcgttgacaa tcactgccac ggcatattgc
     1201 gggaccagac gttcgatgac ctcgcgagct ggcggcagtc cttcaccgag tctacagagc
     1261 ctcaaatggc gcgagaccac gtcgcgagca cgaccttcta ccggcgtctc atccggacgc
     1321 tggccgattt cctgggttgc gagccggaag aagaggtcgt cttcgcagcc aggaccggga
     1381 gggacggacg ggagctcaca ggcgcgttgt tgcgcgccgc gaacgtcgat accttgctca
     1441 ttgacacggg attcccgccg cccgaagagg tgtttcccgt acctgagttg ggagatctcg
     1501 gaggctgccg tgtggagccc atgctgaggc tcgagatcct gatggagagt ttgctcgcgg
     1561 aacacgactc tctggaggat gtgaaagagg cgctcgccgc cgaactggaa gacgcgcggg
     1621 gcaggggcta cgtggcgctc aagagcatcg ccgcttacag gactggcctg gacgttcggg
     1681 agtggccaag cgaggaggcc gaagaatcgt tcctggagta caggcgggct gacggtcctg
     1741 gccctgcgcg cctcgtacac aagccactcc tggataccct gctgcatgtc gcgttcgctc
     1801 aggcggccag gcaggagatt ccgctccagt tccacgtcgg ctacggcgac gccgacaccg
     1861 accttctgct cggcaacccg ctctacctgc gcccggtgct tcagcggcca gactatcgcg
     1921 gcatgcccgt cgtgctgctg cacgaatgct acccgtacac caggcagggc ggctacctgg
     1981 ccgccgtcta ccagaacgtc taccttgatc tctcctacgg catcccattg ctcggctatg
     2041 gcgagatgct ctcctttacg cggcaggcgt tcggcgtcgc cccgagctcc aagctcctgt
     2101 acagctctga cggcataggc gtgcccgagc tacattggat cagcgccata gacggcaggc
     2161 gcgtgatcgg cgaggcactc ggagagctcg tgtctcgagg agagctagac ctcgccgcgg
     2221 ccgaggccgc cggtgaggac gtgctgcgtg cgaacgcgac acgtctgtac gggctctagc
     2281 tcgcgagagc cagggtaggg caggaggtgc gtgagcgtgc gcaaggggtt atcctacccg
     2341 ccagagcctg atcgaggctc ctaaccgaac acggagggga gctttgtacc gacagacacg
     2401 ggtgcttggt gtgctgctca ttcttcaggt gggcgcgatg gtggggatcg gcgtctggtt
     2461 gttcgccagc atcgactggg acacgatagt gaatctttcc aacaagccgg gccagattcg
     2521 gctccaagcc actgacaagg ccgaggtgaa ggatctcaaa cgggcgatcc tgttcgcggt
     2581 ctattttttc gcgcccgtgg tcctgttgct cctggcgggc ctcggcttcg tgctgctcag
     2641 gcgccgcggg tggatgctgg cttcggtcgc
//
LOCUS       DASTUV010000269         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_381623, whole genome shotgun sequence.
ACCESSION   DASTUV010000269 DASTUV010000000
VERSION     DASTUV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381623"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="VFT03_05415"
     CDS             complement(<1..1854)
                     /locus_tag="VFT03_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847616.1"
                     /translation="MAHQRHNVLLDLIRALLVVVAVYLLPGWFWARLLRASADRAEQA
                     AFSVALSMALVPAALVVPTSLFGMGITLAIAVAAPLLVFTAGLVAYLRFGAAKGSDVP
                     LAPPPAPLGTPALALLAAALALAFGVVIGVLPGVPIVPLITVGVVPEMGVLISIIILI
                     VFAGIAQVVESRREPVSPTAETGRDVAWSGTPFALARRLVLPVVLMLALVRGYSGPIL
                     HDWPFMRGVDHYSHAVMAELMMKEGRIEPYLIYPPGFHSMTAELSRLSGLDPLELFPV
                     VAPLLLLLPALALYVVANRLWGWECGVVAALFSVVLGGTYYYYNDAMYPNLVTSQFLM
                     VLAIAALLSMYASAPSVRGGLLLALVGSSVVLYHPVGSMYLAMLLAVVGLVFVPYLYL
                     RDRPKGVALILSLALLGLLSVVYAWGTYDLGEEITALVGGSSASSSTGTAISMAVGTQ
                     LPYSLNDLMGNMVSQPVAWLGLLGVFLVTGELLWRRTTAPQALAYLTLLFWAVLLFVG
                     SRTPLTGFPQRFGRDLCVPLAVFAALALVLVLRSLSDTRRQAVAVFVASLAVVMAGTL
                     VGVRTAASLQIASEPSVQMTITPEISAAGEWLREHNQGGNIMVSPHANQVPS"
     gene            2029..3078
                     /locus_tag="VFT03_05420"
     CDS             2029..3078
                     /locus_tag="VFT03_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HEU4847617.1"
                     /translation="MTAEHVRFAGVIVGALFAAYGIFMYRRGGWSRFGLLLSLLIAVG
                     VALVSVLPQVGEVFTHLFGLENRGFALLSFATLLLFALFLYLLNQVQEARRRSGEIVT
                     GLAVREYGEKYVAPEERPSERREILIVVPAYNEEGGIKEVLRRVPAEILGYEVKIVVV
                     DDGSTDATAAIAHQEGFPIVTHVVNRGQGDALRTGFEIAWREGADIVINLDADGQYKP
                     EEIERLVEPIIKDRADFVLGSRFMGFYEEAGSVRHVGVVFFSKLISLLTGTRISDCTN
                     GFRAIRVSELHKLDLREDRFNATEIILEGLKNKLRFEQVPVTMLSRAAGETKKPPKLA
                     YPLGVFRVIIQTWLR"
     gene            3153..>3373
                     /locus_tag="VFT03_05425"
     CDS             3153..>3373
                     /locus_tag="VFT03_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847618.1"
                     /translation="MRAGSAYVAGSFFISGLLTYVFQGLSARYLGKAGYGDLVMLWSM
                     TFLVVQVLWIGVTQTLGRFVSERLSRGEDP"
BASE COUNT          676 a         1059 c         1097 g          541 t
ORIGIN      
        1 gcttggcacc tggttggcgt gggggctcac catgatgttt ccgccctggt tgtgttccct
       61 gagccactct cccgcggccg atatctccgg cgttatcgtc atctggacgc tcggttcgct
      121 ggcgatctgg aggctggcgg ccgtcctcac acccacgagc gtacccgcca tgaccactgc
      181 cagagaggcg acgaagaccg caactgcctg cctgcgagta tccgacagcg accgcaggac
      241 caataccaag gcgagggccg cgaagacggc gagcggtacg cacagatcgc gcccgaagcg
      301 ctgcgggaag ccggtgagcg gcgtgcggct gcccacgaac agcagcacgg cccagaagag
      361 tagggtgagg taggcgagcg cctgcggagc ggtcgtcctc ctccacagca gctcacctgt
      421 cacgaggaat acccccagca accccagcca cgccaccggc tgcgagacca tgttccccat
      481 caggtcgttg agggagtagg ggagttgggt gcccaccgcc atgctaatgg cggtgcccgt
      541 ggagctcgag gccgaagagc cgccgacgag cgcggtgatc tcctctccca ggtcataggt
      601 gccccaggcg tagacgacgg agagcagccc caaaagtgca agcgagagaa tcaaagccac
      661 gcctttgggc ctgtcgcgca ggtacagata tggtacgaac actagcccca ccacggcgag
      721 cagcatcgcc aggtacatgc tccctacggg atggtagagc accactgagg agcctaccag
      781 ggcgagcagc aacccgccgc gcaccgacgg agcagaggcg tacatactca gcagggccgc
      841 tatggctaga accatcagga actgcgaggt caccaggttc gggtacatcg cgtcgttgta
      901 gtaatagtag gtgccaccaa gcaccacgga gaaaagcgct gccacaacac cacactccca
      961 gccccagagc ctgttggcca ctacgtagag ggcgagcgca ggcagcagca gtagcaaggg
     1021 tgccacaaca gggaagagct cgagcggatc gagaccggat aggcgtgaga gctctgctgt
     1081 catcgagtgg aagccgggag gatagatcag gtagggttcg atcctcccct ccttcatcat
     1141 aagctctgcc atcaccgcgt gggagtagtg gtccacaccg cgcataaaag gccagtcgtg
     1201 gaggattggt cccgagtagc cgcgcacgag cgccagcatg agcacaacgg gcagcaccaa
     1261 ccggcgagcc agcgcgaacg gagttccgct ccaggcgacg tcccgtccgg tttcggcggt
     1321 aggtgagacg ggttcacggc gagactcgac tacctgcgcg atccccgcga agacgatcag
     1381 gatgatgatc gagatcagaa cccccatctc gggcactacc ccgaccgtga tcaagggcac
     1441 tatcggcacc ccgggcaaca ccccgataac taccccgaac gcgagggcca gcgccgcggc
     1501 gaggagcgcg agagctggcg tgcccagtgg agcgggcgga ggggcgagag ggacgtcaga
     1561 gcccttggct gctccaaagc gtagatatgc tacaaggccc gccgtgaata caagtagcgg
     1621 ggctgcgacg gctatggcga gggttattcc catgccgaac aggctcgtcg gtacgacgag
     1681 cgcggcgggg acgagcgcca tcgatagcgc caccgaaaaa gcggcctgct cggcgcggtc
     1741 cgccgatgcg cgcaggagcc gtgcccagaa ccacccgggg aggaggtaga ccgcgaccac
     1801 caccagtaga gcccggatca ggtcgagcag cacgttgtgc ctctgatgtg ccacctcctg
     1861 cgcacgccga tagcttaacc agtatcctct gatgctgcca tcctcacgcc gccacgattt
     1921 acattggagc gaccgtttta tacaatcccg ccgtcacgca cattgcgtga cgaagatcgt
     1981 ggacccgacg gaggcaacct tggttagagc gaccgtggag gatgaaaggt gacggcggaa
     2041 cacgtgcgct ttgcgggcgt gatagtcggc gcgctcttcg cagcgtatgg catcttcatg
     2101 taccgccgcg gaggatggag caggttcggc ctgctcctca gcctgctcat agccgtcggt
     2161 gtggccctgg tctcggtcct cccgcaggtg ggcgaggtct tcacccacct gttcggcctg
     2221 gagaaccgcg gtttcgcgct cctgtcgttc gctaccttgc tgctcttcgc gctgttcctg
     2281 tacctgctca accaggtgca ggaggcgagg cggcgcagcg gcgagatcgt caccggcctc
     2341 gccgtacgcg agtacgggga gaagtacgtg gccccggagg agcgaccctc tgagaggcgc
     2401 gagatactga tcgtcgtccc cgcctacaac gaggaggggg ggataaagga ggtgctccgc
     2461 agggtccccg cagagatcct tggttacgag gtgaagatcg tggtggtaga cgacggctcg
     2521 accgacgcaa cggcagccat cgcccaccag gagggcttcc ccatagtcac gcacgtcgtc
     2581 aaccgggggc agggcgacgc gcttcgcacc ggcttcgaga tagcctggag agagggcgcc
     2641 gacatcgtga tcaacctcga cgccgacggc cagtacaagc cggaggagat cgagcgcctc
     2701 gtcgagccga ttatcaagga cagggccgac ttcgtgctcg gctcgcgctt catgggtttc
     2761 tacgaggagg cgggcagcgt tcgacacgtc ggcgtggtct tcttctccaa gcttatatcg
     2821 ctcctcacgg gcaccaggat cagcgactgc acgaacggct tcagggcgat aagggtctcc
     2881 gagctgcaca agctggacct gcgcgaggac cggttcaacg ccacggagat catcctcgaa
     2941 ggcctcaaga acaagctgcg cttcgagcag gtgcccgtca ccatgctgag ccgcgccgca
     3001 ggcgaaacga agaagccccc gaagctcgcc tacccgctag gggtcttccg ggtgataatc
     3061 cagacgtggc tacgctaggc ttcaggtcta tttgctgata cctgaagcct gaaatctctc
     3121 tgatgaccag tctcctccaa gcacggcagg gggtgcgagc cggcagcgcc tacgtcgcgg
     3181 ggtcgttctt catctccggc ctgctgacct acgtcttcca gggcctctcg gcccgctatc
     3241 tggggaaggc gggctatggc gacctggtca tgctctggtc gatgacgttc ctcgtggtgc
     3301 aggttctctg gatcggggtg acacaaacgc tggggcgctt cgtctcggag aggctctcgc
     3361 gcggcgagga ccc
//
LOCUS       DASTUV010000270         4882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_681222, whole genome shotgun sequence.
ACCESSION   DASTUV010000270 DASTUV010000000
VERSION     DASTUV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681222"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VFT03_05430"
     CDS             <1..1226
                     /locus_tag="VFT03_05430"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU4847619.1"
                     /translation="RYADDGFVVDEELAGWLGSEYSAFPANAKAIYGRGGRPLGAGDL
                     LVQEGLADSLRTIAGGGPGTVYGGRLGQAMVSEVQRGGGFLTLQDLQDNRAQWRETIG
                     IDHMGYRVVTASSPASSWGVLVRLGILGEFDLKPSDHNSVPYVHALTEVSKRTTSHAI
                     EFVDPQTGEARLDKVLSEGFWRREAARIDFSRASPYEPPVSSDSALTCSPTNYTPTSP
                     DTQQHTTHFVVADGEGNVVSATETLGNVFGSKVMPEGTGIWLNDEVAWARFEPAGNPF
                     DAAPGRQIPYALGPTLVLREGRPVIAIGTPGGRTILQITPQMLTNVIDFDMDIQRAIS
                     APRFGFVIPDLLLVEPGMPASVRSGLSAMGHNVYEEPELGNAHALMIEYGPDGKPVRF
                     TGAADPRGGGAAIGY"
     gene            complement(1228..1593)
                     /locus_tag="VFT03_05435"
     CDS             complement(1228..1593)
                     /locus_tag="VFT03_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847620.1"
                     /translation="MASNEMPRPPYVLLFEDVVPGARPLAILVDDRGAVPLFGSEARA
                     QDFLAATDLGSDFEPVEVSRAGLIRALESVADEVEYVALDPPPAAEGGMRVRMGSLRE
                     LVEALETSREDDLFGLGSG"
     gene            complement(1598..1918)
                     /locus_tag="VFT03_05440"
     CDS             complement(1598..1918)
                     /locus_tag="VFT03_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4847621.1"
                     /translation="MESKRVDAEISFSSEKMKKNSLFDSPHLFYDTYCLLPGQSQKVH
                     AHEGSDKVYYVLRGAGRFTVGDEEEDLGEGHAVIARAGIPHGVRNETDENQVLLVTMA
                     PRPS"
     gene            complement(1951..3120)
                     /locus_tag="VFT03_05445"
     CDS             complement(1951..3120)
                     /locus_tag="VFT03_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU4847622.1"
                     /translation="MDSNDERTLLVISAPQHDAAAYHLSGFLAPDPVICLRAAGKKYL
                     AVSSLEYGRAEKEAPVDELISFDELELVKLAQELKSGSRAYAAAAANLIERAGAEGSP
                     VTVPSHFGVAYADELRARGVSVQPDGKLFSELRRVKTEEEISHIEETQRAVERACAHA
                     VGILEEAEISDDGTLRWRGETLTSELLRSEIDIELLRQGCTADEGTIAAGGPQAADPH
                     ESGSGPLRAGETIILDIFPRNQRSRYYADMTRTFVKGEPDEGLQDMYDAVLEAQEAAL
                     SMIRAGVKGRDVHDKVSDILHERGYKTGKHDQMPGEPLTEGFFHGTGHGVGLEVHEAP
                     RISTADEELKAGDVVTVEPGLYEPGVCGVRIEDLVVVTQDGCRNLTSFPKEFRLG"
     gene            3216..3458
                     /locus_tag="VFT03_05450"
     CDS             3216..3458
                     /locus_tag="VFT03_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU4847623.1"
                     /translation="MTEKRDMQSRVEEALDKVRPAMRADGGDARVVACDEDSGSVSIQ
                     MIGACKGCPLSQLDFAYAIESLIKREVPEVREIAAV"
     gene            3776..4513
                     /locus_tag="VFT03_05455"
     CDS             3776..4513
                     /locus_tag="VFT03_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU4847624.1"
                     /translation="MDGTTAVLKLSYPDDSGFHMEAEALRMFAGRGAALLLELDKGRC
                     AMLLERLEPGVPLTTVGDDEEATSIAAGVMRRLWRPVPSDHQFPTVSDWARGFDRLRR
                     SFGGGTGPMPVTLVEEAEGLFSDLLASEEEPVLLHGDLHHHNVLAARREPWLAIDPKG
                     VVGEAAFDTAALLHNPVEVLGKPRPGKVMERRIDLLSEELGLDRERVRGWGLAGAVLA
                     AYWGLEDSGRVWEEALVFARLLSELRG"
     gene            complement(4535..>4882)
                     /locus_tag="VFT03_05460"
     CDS             complement(4535..>4882)
                     /locus_tag="VFT03_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847625.1"
                     /translation="RTISPEEIADSGPPPPAEDSPVKLSRSIVERAVEIRRTLAEDPS
                     EIRVEMCTIGYKACVNDMITLTHLTNENLPNAGVLKGMQLRRARKKATDALAEARKAL
                     PPGALRATRQEQQ"
BASE COUNT          744 a         1602 c         1659 g          877 t
ORIGIN      
        1 taaggtacgc cgacgatggc ttcgtcgtcg acgaggagct ggcaggctgg ctcggttccg
       61 agtacagcgc gttcccggcg aacgcaaaag ctatctatgg caggggcggg aggccgctcg
      121 gggctggaga tctcctcgtg caggagggcc tcgccgactc cttgcggacc atagccggag
      181 gaggtccggg gaccgtttac ggcggccggc tgggccaggc tatggtctcg gaggtccaga
      241 ggggaggcgg tttcctgacg cttcaggacc tgcaggataa cagggcgcag tggcgggaga
      301 ccatcggcat agaccacatg ggctacaggg tcgtgacggc gtcgtccccg gcctcatcgt
      361 ggggcgtgct ggtcaggctc gggatactgg gcgagttcga cctgaagcct tcagaccaca
      421 acagcgtccc ttacgtgcac gctctcacgg aggtgtcaaa gcggacgacc tcgcacgcca
      481 tcgaattcgt ggacccgcag accggagagg cgcggctgga caaggtgctc tccgagggtt
      541 tctggcggag agaggcggcg aggatagact tctcgcgggc ttcgccctac gagccacccg
      601 tctcgtccga ttcggccctt acctgctcgc cgacaaacta cacgcccacc tcccctgata
      661 cgcagcagca cacgacacac ttcgtcgtgg ccgacgggga gggaaacgtg gtgagcgcga
      721 ccgagacgct gggcaacgtc ttcgggagca aggtaatgcc cgaagggaca ggtatctggc
      781 tcaacgacga ggtcgcgtgg gctcgcttcg agcctgcggg caaccccttc gatgccgcgc
      841 cggggcgtca gatcccctac gcgttaggcc cgacgctcgt gctgcgcgaa gggcggccgg
      901 tgatcgccat agggacaccc ggcggccgca ctatcttgca gattacgccc cagatgctca
      961 ccaacgtcat cgacttcgac atggacatac agcgggctat ctccgcgccc cgctttggct
     1021 tcgtcatacc ggaccttctc ctggtcgaac ccgggatgcc cgcgtcggtt cgctccgggc
     1081 tctccgcgat gggtcataac gtctacgaag agcccgagct cggcaacgcc cacgccctca
     1141 tgatcgagta cggccccgac ggtaagcccg tcaggttcac cggcgccgcc gacccgcgcg
     1201 gcgggggcgc cgcgataggc tactgagtta gcccgagccg agcccgaaca ggtcgtcttc
     1261 ccggctcgtc tcgagcgctt ccactaactc gcgcagggag cccatccgca ccctcatgcc
     1321 gccctctgcg gctggcggcg ggtcgagggc gacgtattcg acctcgtccg caaccgactc
     1381 gagcgcccgg atcaggcccg ctctcgaaac ctcgaccggc tcgaagtcgg atccgagatc
     1441 ggtcgccgcc aggaagtcct gcgccctcgc ttccgagccg aagagcggga cggcaccgcg
     1501 gtcgtctacc aggatggcga gtggcctggc gccggggacc acatcctcga agaggagcac
     1561 gtagggggga cgcggcatct cgttcgaagc cacgagctca ggagggtctt ggggccatcg
     1621 tgacgaggag gacctggttc tcatccgtct cgttgcggac gccgtggggg atgcccgcgc
     1681 gggcgataac tgcgtgccct tcgccgaggt cctcctcctc gtcgccgacg gtgaaacgac
     1741 cggcgcccct gaggacgtaa tacaccttgt ccgagccctc gtgggcgtgg accttctggg
     1801 actggcctgg caggaggcag taggtatcgt agaagaggtg gggcgagtcg aagaggctgt
     1861 tcttcttcat cttctccgag gagaaggaga tctccgcgtc aacgcgcttg ctctccacag
     1921 ttatcctccg atccctgggt gtctgtcccg ctacccgagc ctgaactcct tggggaagct
     1981 cgtcaggttg cggcagccgt cctgggtgac gacgacgaga tcctcgatgc ggacgccgca
     2041 cactcccggc tcgtagagac ccggctcgac cgtgacgacg tcgccggcct tcagttcctc
     2101 gtcggcggtc gagatgcggg gggcttcgtg gacctccaga cccacgccgt gcccggtgcc
     2161 gtggaagaag ccctcggtca gcggctcgcc cggcatctgg tcgtgcttgc cggtcttgta
     2221 acccctctcg tgcagtatgt ccgagacttt gtcgtggacg tcgcggccct tcaccccggc
     2281 ccttatcatc gagagcgccg cctcctgggc ctcgagcacg gcgtcgtaca tatcttgcag
     2341 tccttcgtcc ggctcgccct tgacgaaggt gcgggtcatg tcagcgtagt agcggcttct
     2401 ctggttccgg gggaagatgt cgaggatgat cgtctctccc gctctcaagg gcccactgcc
     2461 gctctcgtgc gggtcggcgg cctgcggccc tcccgcggct atggtgccct cgtcggcggt
     2521 gcatccctgc cgcaggagct cgatgtctat ctcggagcgc agcagctcgc tcgtcagggt
     2581 ctccccgcgc catctcagag tgccgtcgtc cgagatctcc gcttcttcga gtatcccgac
     2641 ggcgtgggcg caggcccttt cgaccgccct ctgggtctcc tcgatgtgag agatctcctc
     2701 ttccgtcttg acgcgccgca gctcggagaa cagcttgccg tccggctgca cactgacccc
     2761 gcgagccctc agctcgtcgg cgtaggctac cccgaagtgc gacggtacgg taaccggcga
     2821 gccctcagcc ccggccctct cgatgaggtt ggccgcggcc gcggcgtagg cgcgagagcc
     2881 gctcttcaac tcctgcgcga gcttcacaag ctccagctcg tcgaaggaga tcagctcgtc
     2941 caccggcgcc tccttctcgg ctcgcccgta ctccagggac gagacggcga ggtacttctt
     3001 gcctgccgcc cggaggcaga tcacggggtc cggcgccagg aacccagaga ggtggtaggc
     3061 cgccgcgtcg tgttgcggcg cgcttatgac gaggagggtt cgctcgtcgt tgctatccat
     3121 gcggctcctt ccagaggaag cttccgggag cccccattct actaggctca ccgcgcgcaa
     3181 tccgtttcgt ggcctcgtat ataatcggtt cgagcatgac cgagaagcgc gacatgcaga
     3241 gccgggtcga ggaggcgctg gacaaggtgc gcccggcgat gagggccgac gggggtgacg
     3301 ccagggtcgt cgcgtgcgac gaggatagcg gttcggtcag catccagatg atcggggcct
     3361 gcaaggggtg cccgctctcg cagctcgact tcgcctacgc tatagagagc ctgatcaagc
     3421 gcgaggtccc cgaggtacgg gagatcgccg cagtctagag acggttcccg tttgcgccgc
     3481 ctgcattcga aggctatcgt tggggccatg atctgacgca cgatggcgcc ccgttctcct
     3541 ccgctgaaga caccctgtcc aagtcgtcat tgccgagagg agatccagca cgcgcgttcc
     3601 gccttccatc cccgacgagc tggcccgcac caccatcgag ctgcgtgggg cggcgggcgt
     3661 cgagtggctc gagcgactgc cggcgctagt tgccgcgtgc ggggagcgct ggtcactcga
     3721 ggtaggccct ccgttctcgc aactctcgtt caactacgcg gctcctgccc ggcgcatcga
     3781 cggcaccacc gccgtcctca agctctctta cccggatgat tccggcttcc acatggaggc
     3841 agaggcactc cgtatgttcg cagggcgtgg ggccgccctg ctgctcgaac tagacaaggg
     3901 gcggtgcgcg atgctgctcg agcgcctcga gcccggcgtg cccctcacga ccgtagggga
     3961 tgacgaggag gcgacctcca tcgccgcggg cgtgatgagg cggctgtggc ggccggtacc
     4021 gagcgatcac cagttcccga cggtgtccga ctgggcgcgg ggcttcgacc gtctgaggcg
     4081 tagctttggt ggagggaccg gtcccatgcc cgtgacgctt gtcgaggagg ccgagggtct
     4141 cttctcagac ctccttgctt ccgaggagga gccggttctg ctgcacggcg accttcacca
     4201 ccacaacgtc ctcgcggcgc ggcgcgagcc gtggctcgcg atcgacccga agggcgtcgt
     4261 gggcgaggcg gccttcgaca cggccgcgtt gctgcataac cccgtggagg tgttggggaa
     4321 gccccggccc ggcaaggtca tggaacggcg catagatctt ctctcggaag agctcggtct
     4381 cgaccgcgag cgggtgcgcg ggtgggggct cgccggggcg gtgctggcgg cctactgggg
     4441 cctcgaggac agcggacggg tgtgggagga ggcgctggtc ttcgcgcggc tgctttccga
     4501 gctcaggggc tagcgccgga gatcccgcgt aaggctactg ctgttcctgg cgcgtggcgc
     4561 gcagggcgcc cggaggcagg gctttcctgg cctcggcgag agcgtccgtg gccttcttgc
     4621 gggcccttcg cagctgcatc cccttgagga cgcccgcgtt aggtaggttc tcgttggtga
     4681 ggtgggtgag cgtgatcatg tcgttcacgc acgccttgta gcctattgtg cacatctcga
     4741 cccttatctc cgaggggtct tcggctagag tgcgccggat ctcgaccgcc cgttcgacga
     4801 tggagcggga gagctttacg ggcgagtcct cggccggggg cgggggtccg gagtcggcga
     4861 tctcctcggg agagatcgtg cg
//
LOCUS       DASTUV010000271         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_861066, whole genome shotgun sequence.
ACCESSION   DASTUV010000271 DASTUV010000000
VERSION     DASTUV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861066"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /gene="pdxA"
                     /locus_tag="VFT03_05465"
     CDS             <1..736
                     /gene="pdxA"
                     /locus_tag="VFT03_05465"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU4847626.1"
                     /translation="DAAFRYLERATGLASAGRVAAIATAPLNKEAMHLAGHRYPGHTE
                     ILADLTGTENYAMMLVTDELKVIHVSTHVSLREAIHRVQPERELAVIRLAHASMRRLG
                     VENPKVAVAGLNPHAGENGLFGTEDAERIAPAILEARTEGIEASGPHPPDTVLMRARR
                     GAFDIVVVQYHDQGHIPVKLMGFDTGVNVTVGLPFFRTSVDHGTAFDIAGTGEADHAS
                     MRAALDLARTLAETEAEAEERAEERS"
     gene            741..2060
                     /locus_tag="VFT03_05470"
     CDS             741..2060
                     /locus_tag="VFT03_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEU4847627.1"
                     /translation="MRFPKVVKVRQDFPRPRVDDVGEALREQCAKEEIRRRIEPGAEV
                     AITAGSRGVAGIDDVLRSLVRILKEAGASPFIVPSMGSHGGATAEGQVEILRSLGVTE
                     ESVGAPIRSSMDVVEIGETGSGVPVYMDRIASEADGVVIVGRIKQHTDFRSGVESGLL
                     KMASIGLGKHAQALALHAHGVKGIRDYMVEAGKVVYESGKVLFGVAIVENAYEETAII
                     EAIPPERVAEREAELLGESAKLMPKLPVSDIDVLFVDELGKNFSGTGMDTNVIGRFKI
                     LGVEEPGSPDVKYLIVGDVSEASHGNALGIGLADFTTRRLFEKIDYEAMNQNVLTSTF
                     VERAKIPMVMENDRDALEAAVRCNWGVEPEDTRFVRIPNTLHLRYAYLSENLLDEALA
                     NGNVEVVEEAADLAFDESEDLVPFDAGAEEQAVAVSSNRDDGYYGGD"
BASE COUNT          425 a          680 c          737 g          316 t
ORIGIN      
        1 cgacgcggcg ttccgctacc tggagagggc gaccggactg gccagcgcag ggcgtgtggc
       61 cgcgatagcg accgctcccc tgaacaagga ggccatgcac ctcgcggggc acaggtaccc
      121 ggggcacacg gagatcctgg ctgacctcac gggcacggag aactacgcga tgatgctcgt
      181 caccgacgag ctcaaggtca tacacgtcag cacccacgtc tctttaagag aggccataca
      241 tagggtgcag cccgagcggg agctagccgt gatcaggctc gcccacgcct cgatgcgcag
      301 gctcggcgtc gagaacccga aggtagccgt agccggcctg aacccccacg ccggcgagaa
      361 cggcctgttc ggcaccgagg acgcggagcg catagccccc gccattttgg aagcgagaac
      421 ggaggggata gaggcctccg gcccccatcc gcccgacacg gtcctgatgc gggcgcgtag
      481 aggcgccttc gacatcgtgg tggtgcagta ccacgaccag ggacacatac ctgtgaagct
      541 gatgggcttc gacaccggcg tgaacgtcac tgtgggcctg ccgttcttcc gcaccagcgt
      601 ggatcacggc acggccttcg acatcgccgg cacaggcgag gccgaccacg cgagcatgcg
      661 cgccgccctc gacctcgccc gaacgcttgc ggaaactgag gctgaggccg aggagcgcgc
      721 cgaggaaagg agctagggag atgcggtttc cgaaggtcgt caaggtcagg caggacttcc
      781 cgaggccccg cgtggatgac gtcggggagg cgctgcggga gcagtgcgcg aaggaggaga
      841 taaggcgccg gatcgagccc ggtgctgagg tcgctataac agccgggagc cgaggcgtcg
      901 cggggataga cgacgtcctg cgctcgctgg tccggatcct gaaggaagcc ggagcttcac
      961 cgtttatcgt cccgtctatg ggtagccacg gcggggcgac ggcggagggg caggtggaga
     1021 tcctgcgcag cctcggggtc acggaggagt cggtcggggc gcccatccgc tcctcgatgg
     1081 acgtggtcga gatcggggag accgggagcg gggtacccgt gtacatggac cgcatagcct
     1141 cggaggcgga cggggtcgtg atcgtcggca ggatcaagca gcacaccgac ttccgctccg
     1201 gcgtggagag cggcctcctg aagatggcct ccatcggcct cggcaagcac gcccaggccc
     1261 tcgccctgca cgctcacggc gtcaagggca taagagacta catggtcgag gcgggcaagg
     1321 tggtctacga gtccggcaag gtgctcttcg gcgtagcgat agtagagaac gcctacgagg
     1381 agaccgcgat catagaggct ataccccccg agcgcgtcgc cgaacgcgag gcggagctgc
     1441 taggagagtc cgcgaagctc atgccgaagc tccccgtctc cgacatcgac gtcctcttcg
     1501 tggacgagct cggcaagaac ttctcgggca ccggcatgga caccaacgtc atcggccgct
     1561 tcaagatcct cggggtagag gagcccggga gccctgacgt gaagtacctc atcgtcggcg
     1621 acgtcagcga ggcgtcgcac ggcaacgctc tgggtatcgg tcttgccgat ttcacgacgc
     1681 gaaggctctt cgagaagata gattacgagg ccatgaacca gaacgtcctc acgagcacgt
     1741 tcgtcgagcg ggccaagatc ccgatggtca tggagaacga ccgggatgca ctggaggccg
     1801 ctgttcgctg caactggggc gtcgagccgg aagacacccg cttcgtccgc atccccaaca
     1861 ccctccacct ccgctacgcc tacctctccg agaacctcct cgacgaggcc ctcgcgaacg
     1921 gcaacgtgga ggtcgtcgaa gaggcggccg atctggcttt cgacgagagc gaggatcttg
     1981 tgcccttcga tgccggggcc gaagaacagg ccgtggccgt ctcctccaac cgggacgacg
     2041 ggtactacgg aggcgactag cgtcccggga gaccactcac cgacactagt taggaacgag
     2101 catgcaactt ttcgagaagc tcatagagca ccccgaggcc gtgcggctgg tgctcgag
//
LOCUS       DASTUV010000272         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1561426, whole genome shotgun sequence.
ACCESSION   DASTUV010000272 DASTUV010000000
VERSION     DASTUV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561426"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1258
                     /locus_tag="VFT03_05475"
     CDS             356..1258
                     /locus_tag="VFT03_05475"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU4847628.1"
                     /translation="MIPTKEGARATATKAVIIGNPNSGSAGDEGYLERFAEALSAGGL
                     EVEVLKTERPDHATELASMSGDQLVVAAGGDGTVNEVLNGLHKDATLGILPLGTANVL
                     ARELGLPLKPEDACERILSGTAFRMDVGVATAEEGTERRFACMAGIGFDANVVREVTP
                     RFKRYLRSLAFPLVALKVYLRGDLPELRLVTGQTTHVGQLAVVANGHYYGGDFKVAEE
                     ASLTSGELEVVLVEKVGRLLRADILARVLAKRPLDRDAKSFPARDIRAESSGGRVPVQ
                     IDGELWGNLPMFFRIEPAAIRVIR"
     gene            1783..>2806
                     /locus_tag="VFT03_05480"
     CDS             1783..>2806
                     /locus_tag="VFT03_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4847629.1"
                     /translation="MYLKTKTVATLSIDTIRALAMDAVQKAASGHPGTPMSMAPVAYT
                     IYTKFLRHNPENPDWKDRDRFVLSAGHASMLQYALLHLTGYDLTMEDIKHFRQWESRT
                     PGHPEHFLTPGVETTTGPLGQGFANGVGMALAERFLAGRYNRIGHEIVDHHIYAICSD
                     GDLMEGVAHEAASIAGQNGLGKLIYVYDDNRITIDGTTALSFDAEEKGQRFEAYGWHV
                     QRVEDSEDLEALEDALANARDEEERPSLVIVRSHIAYPAPHAQDTAAAHGSALGEEEV
                     RATKERMGLDPDEHFYVSDEVYEHMNMVGRGRDLEDEWNGRFEAWREEFPELVEEWER
                     AWSGRPE"
BASE COUNT          567 a          858 c          907 g          474 t
ORIGIN      
        1 tagaacacgc ttacgcccac agtcagatgc aaaccctagc ctatttggag gcggtcatgg
       61 aagtcgtgga gttcgtgatt gagatggccg agtggaggaa ggctctcacc gttgggtagg
      121 gggtgcctgt tctggatagc tgtcagcatc atcttgagcg tcggcctaac tattatcctt
      181 aaccttggcc tcttcgcgct cggcgggtag cggatgatac gttgggggag gggcaatagc
      241 cgggggtgct gcgtggtcgt gcccatcggg tgtctgctgt ccgtgacact gatcttggcg
      301 ttctccggac taaccgtcac cctactactc tagaagagag tcgtaagacc ctaagatgat
      361 acctacgaag gagggggctc gcgccacggc aacgaaggcg gtcatcatcg gcaaccccaa
      421 ctcggggagt gcgggcgacg agggttacct ggagcgcttt gccgaagccc tgagcgccgg
      481 cggccttgag gtcgaggtgc taaaaaccga gcgcccggac cacgccaccg agctcgcctc
      541 catgtccggc gaccagctcg tcgtcgccgc ggggggcgac gggacggtga acgaggtctt
      601 gaacggcctc cacaaggacg ccaccctggg catcctgccg ctcggcacgg cgaacgtgct
      661 cgctcgcgag ctcgggctgc cgctgaagcc cgaggacgcc tgcgagagga tcctgagcgg
      721 caccgccttc cggatggacg tcggggtggc cacagccgaa gaaggaacgg agcggcgctt
      781 cgcctgcatg gccggcatcg gcttcgacgc caacgtcgtc cgcgaggtga cgccgcgctt
      841 caaacgctac ctgaggtcgc tcgccttccc gctcgtcgcg ctcaaggtgt acctccgggg
      901 cgacctgccg gagctgcgcc tcgtaaccgg gcaaacgacc cacgtggggc agctggccgt
      961 cgtcgccaac gggcattact acgggggcga cttcaaggta gccgaagaag cctccctcac
     1021 gagcggcgag ctggaggtcg tcctggtcga gaaggtaggt cgtctcctgc gggccgacat
     1081 ccttgcccgc gtgctggcga agaggccact ggaccgggac gcgaagtcct tcccggctcg
     1141 ggatatccgg gcggagtctt cgggcgggcg tgtcccggtg cagatagacg gcgagctctg
     1201 gggcaatctg ccgatgttct tccgcataga acccgcggcc ataagggtga tcaggtagct
     1261 gagaacatct gcaagaacca ggattgctcg tggcccggtg ttcgagatac cagggaggca
     1321 ggcggccctt ggtcacctac ggaacggctc ctgcgcgagc cccgctagtc gcttactgtg
     1381 cccggccgga tgaggctttt ctaggcgcca tcacctgcta ccaaaaaagg agtgctagca
     1441 aggccataac tacctatagg atgatttcgt cagcaccgag cgcgagcaga gtcgccgcac
     1501 tcttggcgta cctgggaacc acggcgacga atccgccgca gtgacgtttg aggagcatcg
     1561 cgagctgaaa agccgatttg gccgaaccac ccggcgattc gatgactaga gcaatcttct
     1621 cctttggcag actgtcttgc gctcgaaaga actgagtagc aagaaaatag attaatccct
     1681 gccggaagaa gggtatatga gagctagtta gcatggatct atccacctca gctaccgtgg
     1741 caaacataac gggaggctgc cgagcgctaa gaaaggaacg acgtgtacct caaaaccaaa
     1801 accgtcgcga cgctatcaat agacaccatc cgagcgctgg cgatggatgc ggtgcagaag
     1861 gcggcctctg gccacccggg gacgccgatg tcgatggcgc cagtagccta caccatctac
     1921 accaagttcc tgcgccacaa ccccgagaac ccggactgga aggatagaga ccgtttcgtt
     1981 ctctccgccg ggcacgcgtc catgctccag tacgccttgc tccatctcac gggctacgac
     2041 ctcacgatgg aggacatcaa gcacttcagg cagtgggaat cgaggacgcc gggccacccg
     2101 gagcacttcc tcacccccgg cgtcgagacg acgaccggtc ccctgggaca aggcttcgcc
     2161 aacggcgtcg ggatggcgct ggctgagcgt ttccttgcgg gccgatacaa taggattggg
     2221 cacgagatag tcgatcacca catctacgcc atctgctccg acggcgactt aatggagggc
     2281 gtcgcccacg aagcggcctc catcgcgggt cagaacggcc tcggcaagct catctacgtc
     2341 tacgacgaca accgcattac catcgacggc accaccgccc tctccttcga cgccgaggag
     2401 aagggccagc gcttcgaggc ctacggctgg cacgtccagc gcgtagagga ttcagaggat
     2461 ctggaagctc tcgaagacgc attggctaac gcccgcgacg aggaggagcg gccgtcctta
     2521 gtgatcgtga ggagccacat cgcctacccc gccccgcacg cccaagacac cgccgcggcc
     2581 cacggctcgg ccctgggcga ggaggaggtt cgggcgacca aggagcggat gggcttggac
     2641 cccgacgagc acttctacgt ctcggacgaa gtctacgagc acatgaacat ggtcgggcga
     2701 ggtcgcgacc tggaggacga gtggaacggg cgctttgagg cttggcgcga agagttcccc
     2761 gagctggtcg aggagtggga gcgggcatgg agtggtcgtc ccgagc
//
LOCUS       DASTUV010000273         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_913476, whole genome shotgun sequence.
ACCESSION   DASTUV010000273 DASTUV010000000
VERSION     DASTUV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913476"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VFT03_05485"
     CDS             complement(<1..922)
                     /locus_tag="VFT03_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4847630.1"
                     /translation="MEFRAELDPLAPYKPPRMSQEEAAERGTDGYVKLTSNELSFGPL
                     PEAEAALAEALPRANRYPDRHASSLREAVASANPGADSRKNVLVGNGSSEVLLNLLQL
                     VRRPGEVVFPWPSFGLYVSISVTLGLSPKKVQLTGRHEAKPEALLSAVTAKTRAVILS
                     NPNNPTGTYLTLDEVRGLADALPESVFLILDEAYHEFVSDPAYHGSHELALERENVVC
                     VRTFSKAHGLAGFRVGYGIATERVADYVERVRFPFSVNLAGQVAATASMRASEKIRER
                     AGFVIRERERVQDAFREAGFDYVQSQGNFVL"
     gene            1107..1403
                     /locus_tag="VFT03_05490"
     CDS             1107..1403
                     /locus_tag="VFT03_05490"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU4847631.1"
                     /translation="MEEAALQRQVRELRARVDEVDRELIRALNERARIVQEIMALKSE
                     AGAPVYDPKREEEILRKVVEMNTGPIYDSSMRDIFELIMHRIRDLEIQRGEFSR"
     gene            1406..>2118
                     /gene="aroF"
                     /locus_tag="VFT03_05495"
     CDS             1406..>2118
                     /gene="aroF"
                     /locus_tag="VFT03_05495"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4847632.1"
                     /translation="MDVSQSVKTDVSTANGMMLPGTFRVVAGPCTVESYEQFLAGAKA
                     VKQAGFEYVRGGAFKPRTSPYSFQGLGEEGLKIMDDVSRDLGLKVVTEVMDPYDIEMV
                     MEHAQVLQIGARNMANFSLLKRVGAAIAGTDHGVVLKRGLSATVEEWLLAAEYVTSSG
                     GDNVVLCERGIRTFETSTRFTLDLSAVIVAKRLTDLPVIVDPSHAAGRRDLVVPLSKA
                     AVAAEADGLIVESHHDPSEA"
BASE COUNT          369 a          647 c          721 g          381 t
ORIGIN      
        1 cgagaacgaa gttgccctgc gactggacgt agtcgaaccc cgcctctcgg aaagcatctt
       61 gcacgcgctc gcgctcccgg atcacgaacc cggcgcgctc cctgatcttc tcgctggcgc
      121 gcatcgaggc ggtggcggcg acctggccgg cgaggtttac ggagaacggg aacctgaccc
      181 tctccacgta gtcggcaacc cgctcggtag ctatcccgta gccgaccctg aagccagcta
      241 gcccgtgcgc cttcgagaac gtgcgcacgc ataccacgtt ctccctttcg agggcgagct
      301 cgtgcgagcc gtgataggca gggtccgaga cgaactcgtg gtaggcctcg tccaggatga
      361 ggaagacgct ctcgggaagc gcgtcggcta ggccgcgcac ttcatccagg gtcaggtacg
      421 tgcccgtggg gttgttcggg ttcgagagga tgacggcccg cgtcttcgcc gttactgcgg
      481 agaggagtgc ctcaggcttc gcctcgtgcc gccccgtgag ctggaccttc ttcggtgaga
      541 gccccagcgt aacgctgatg ctcacgtaca ggccgaacga tggccaggga aagactacct
      601 cgcccggacg ccggacgagc tggaggaggt tgaggagcac ctcgctggac ccgttgccca
      661 cgagcacgtt cttccgggag tcggcgccgg ggttggctga ggcgacagcc tcgcgtaaag
      721 agctggcgtg gcggtctggg tagcggttgg cccggggcag ggcttcggcg agcgcggcct
      781 cggcctcggg gagcgggccg aaggagagct cgttcgaggt cagcttcacg tagccatccg
      841 tcccgcgctc cgccgcctcc tcctgcgaca tgcgcggcgg cttgtatggg gccagcgggt
      901 ccagctccgc tctgaactcc atgcgctcct ttcaagctgg tcagcacatt gtttcgccga
      961 tcgaggtcgc taaagactat tcctctttaa ttggcctttc aacgggcgtt ggtgcgatcg
     1021 ggtgagtttc ggctctgctt cttgctgacg agctcgaagc cgctggtttg ccttatgtgc
     1081 cgtgtacaga tagaatcgca ggttctatgg aagaggcagc gttgcagagg caggtgcggg
     1141 agcttagagc gagggtcgac gaggtcgacc gggagctcat ccgggccctg aacgaacggg
     1201 cgcgcatcgt gcaggagatc atggcgctga agtcggaggc gggggccccg gtctacgacc
     1261 cgaagcgtga ggaggagatc ctgcgcaagg tcgtggagat gaacacgggt cccatctacg
     1321 actcctccat gcgcgacatc ttcgagctga taatgcaccg catcagggac ctcgagatcc
     1381 aacgcgggga gttttccagg tagaaatgga cgtttcacag agcgtaaaga ccgacgtaag
     1441 caccgccaac ggcatgatgc tccctggtac gttccgggtc gtcgccggcc cctgcacagt
     1501 cgagagctac gagcagtttc tggccggggc gaaggccgta aagcaggcgg gcttcgagta
     1561 cgtccgcggc ggcgccttca aaccccgcac ctcaccttac tccttccagg gcctcggcga
     1621 ggagggcctg aagatcatgg acgatgtgag ccgggacctg ggactcaagg tcgtcaccga
     1681 ggttatggac ccctacgaca tagagatggt catggagcac gcgcaggtcc tgcagatcgg
     1741 ggcccgcaac atggcgaact tctcgctgct gaagcgggtc ggcgctgcta tcgcgggcac
     1801 ggaccacggc gtcgttttga agagggggct ctcggcgacg gtcgaggagt ggctgctcgc
     1861 cgcggagtac gtgacctcct ccggcgggga caacgttgtc ctttgcgagc ggggcatccg
     1921 tacgttcgag acgagcacgc gctttacgct ggatctctct gccgtcatcg tcgccaagag
     1981 gcttacggac ctgccggtga tcgtggaccc ctcgcacgcc gcgggacgta gggacctggt
     2041 cgtgccgctg tccaaggccg ccgtagccgc ggaggctgac ggcctgatag tcgagagcca
     2101 ccacgatccc agcgaggc
//
LOCUS       DASTUV010000274         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1396303, whole genome shotgun sequence.
ACCESSION   DASTUV010000274 DASTUV010000000
VERSION     DASTUV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396303"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFT03_05500"
     CDS             <1..523
                     /locus_tag="VFT03_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847633.1"
                     /translation="VDQFPDAVNGVWLALVLIATSTAIGIAVLKYRLYDIDVVINRTL
                     VYGPLTATLALVYLGGVISLQYAFRALTGQGSQFAIVASTLAIAALFNPLRRRIQALV
                     DRRFYRRKYDARKTLEGFSARLRDETDLAALNAELVSVVRQTVQPAEVSLWLRPDPAA
                     KSGGGEETGGQHH"
     gene            556..870
                     /locus_tag="VFT03_05505"
     CDS             556..870
                     /locus_tag="VFT03_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847634.1"
                     /translation="MYEELTSGNKVVDLSPPQALDRAESFLVGQGYVVVQRTATTLTV
                     EREGSRSAAGQEGAPNVVVMAVPQLDGGVRIKVRGSDREGLQERQGLWNLWAENLPKR
                     QH"
     gene            complement(929..1420)
                     /locus_tag="VFT03_05510"
     CDS             complement(929..1420)
                     /locus_tag="VFT03_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEU4847635.1"
                     /translation="MTEEQRNYGVEPEPGGPGRSEPDGSRLRSTHSNSTISPSDERTW
                     SVLAHLSVFLNLFTGILGPVAALVIWLMYKDKSSRVSFHALQSLWYQLAWMVILAIGW
                     TVAGILTVVLIGFLLFPVMIVATLVPFVHMGYAAYKVSQGVDYRYPFVADLIDENRPA
                     NTV"
     gene            complement(1620..>2644)
                     /locus_tag="VFT03_05515"
     CDS             complement(1620..>2644)
                     /locus_tag="VFT03_05515"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU4847636.1"
                     /translation="TYAVLVGSSPVVAIYALAFGTTLGVAVMSLVLVPPAWRLGYRPQ
                     VAFGHPALLATLRLAAPVLVFTAATVGVQMALNLFGSRFGGVEKLYYAFVVFQLPYGV
                     FVVAIATALVPELSERFTRGDPDGFREHLSFGLRMTAFIAVPASVMLVVLAEPIVGLL
                     YEHGSFTRQDTAEVAALVAAFGVGLLGYAFSFVLARSFYSRQNARTPALLNTGLLAMY
                     VALAFTLSRTMELTGVALAFSIAYAVLALALLAAMRGEIKRLDGRYLLWSLVKIFAAG
                     AAMYGVARGGLWLMGPGDGLVDSAVVLIFVGGAALATYSVTSLLLKVEELGSAATLLR
                     KRSASP"
BASE COUNT          549 a          793 c          860 g          442 t
ORIGIN      
        1 ggtggaccaa ttcccggatg ccgtcaatgg agtgtggctc gctctggtgc tcatcgccac
       61 atccacggca atcggaatcg cggtgctgaa gtatcgcctc tacgatatag acgtcgtaat
      121 aaaccgcacc ctcgtctacg gtcctctcac tgcgacgctc gccctcgtct acctcggtgg
      181 cgtgatctcc ttacagtacg ccttccgcgc gctcaccgga caggggtctc agttcgccat
      241 cgtcgcctcc actctggcca tagccgccct gttcaacccg ctgcgaaggc gcattcaagc
      301 gttagtggat cggcgcttct accgccgcaa gtacgatgcg aggaagacgc tcgaaggctt
      361 ctcggccagg ctcagagacg agaccgacct ggctgccttg aacgccgagt tagtatcagt
      421 agtgaggcaa acggtgcagc ctgcggaagt gtcgctgtgg ttgcggcccg atcccgccgc
      481 aaagagcggt ggaggcgagg agacaggagg acagcaccat taaatttggc gtgctgcgat
      541 tcggggaagg ggcgtgtgta cgaagagctg acctccggca acaaagtcgt tgacctttcg
      601 ccgccacagg cgctagacag agcagagtcc ttcctggtgg ggcagggcta cgtcgtagtg
      661 cagcgcactg ccaccacgct cacggtcgag cgggaaggtt cccggagcgc agctgggcaa
      721 gaaggcgcgc cgaatgtggt ggtcatggcc gtgcctcagc tcgacggtgg ggtgaggata
      781 aaggtgaggg gcagcgaccg cgagggcctg caagagcggc aaggtctgtg gaacctgtgg
      841 gcggagaacc tgccgaaaag acaacactaa tgcgctgtgc aaatggagta ctccatttgc
      901 acagcacacc gcctccttgt gatttggcct atacggtgtt ggccgggcgg ttctcgtcga
      961 tcagatccgc cacgaagggg tagcgatagt ccactccctg gctgaccttg taggcggcat
     1021 atcccatatg cacgaagggc accagggttg ccactatcat caccgggaag agcaggaagc
     1081 cgatcagcac gactgtcagg atgcccgcca ccgtccagcc tatggccaga atcaccatcc
     1141 aggcgagctg gtaccacaag gactgcaggg cgtggaagga gaccctggaa gatttgtcct
     1201 tgtacatcag ccagatcacg agcgccgcca ccggacccag aattccggtg aagaggttca
     1261 agaacaccga gagatgggcc aggacagacc aggtcctctc gtcgctcggg ctaatggtcg
     1321 agttggagtg ggtgctcctc agcctcgatc cgtcgggctc cgaccttccc ggccctccgg
     1381 gctccggctc gacgccgtag tttctctgct cttcggtcat cgggttactc ctctcgtcgt
     1441 gtctatccta atctacgtac tgagggttct ggggttgcgc cgccgctatg agtatatggt
     1501 gctcgaaagc cccagggccg aatgcgcagt aagagggggg tagcctccag gctactgaga
     1561 gagaggcctg atccactcca ccaagtgtgc ctacttcttg gaccacactc gtagatcttc
     1621 taagggctcg ccgaacgctt gcgcagtagc gtggcggcag aaccgagctc ttcgaccttg
     1681 agcagaagcg acgttacgga gtaggtcgcg agcgcggcac caccgacgaa gatcaaaact
     1741 accgcgctat ctacgagccc gtcccctggc cccatgagcc agagacctcc tcgggcgacg
     1801 ccgtacatgg ctgctccggc cgcgaagatc tttaccaacg accacagtag gtatcgccca
     1861 tccagccgct ttatctctcc tcgcatagcc gcgagcaggg cgagggcgag cacggcgtag
     1921 gctatggaga acgcgagcgc gacgccggtt agttccatcg ttcgcgacag cgtgaaggcg
     1981 agcgctacgt acatcgccag gagcccggtg ttcaggagcg cgggcgtcct cgcattctgg
     2041 cgcgagtaga aagagcgggc gagcacgaag gagaatgcgt aaccgagcag gccgacgccg
     2101 aacgcggcaa ctagagcggc gacctccgca gtgtcctggc gagtgaagct gccgtgctca
     2161 tagagcaggc cgactatggg ctccgcgagg acgacgagca tgacggaggc cgggaccgcg
     2221 atgaaggcag tcatcctgag cccgaaggag aggtgctcgc ggaaaccgtc agggtcgccg
     2281 cgggtgaagc gctccgagag ctcgggtacg agggctgtgg cgatcgcgac gacgaagacg
     2341 ccgtaaggga gctggaagac gacgaaagcg tagtagagct tctcgacgcc gccgaagcgg
     2401 gatccaaaga ggttgagagc catctgcacg cccaccgtcg ccgccgtgaa gacgagcacg
     2461 ggagcggcaa gccgtagcgt cgcgagcagg gcgggatgcc cgaaagccac ctggggcctg
     2521 tagccgagcc gccaggcagg cgggacgagc acgagcgaca tcaccgcgac cccgagcgtc
     2581 gtcccgaagg cgagagcgta gatcgctacc acagggctgc tcccgacgag caccgcgtag
     2641 gttg
//
LOCUS       DASTUV010000275         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1314345, whole genome shotgun sequence.
ACCESSION   DASTUV010000275 DASTUV010000000
VERSION     DASTUV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314345"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFT03_05520"
     CDS             complement(<1..199)
                     /locus_tag="VFT03_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HEU4847637.1"
                     /translation="MQDVDQTQQDETRREPLPFEEVERLVDLVRRSGVGEIRVRRGDV
                     EISVKARDESAAATGAPRGAAQ"
     gene            467..2251
                     /gene="mutL"
                     /locus_tag="VFT03_05525"
     CDS             467..2251
                     /gene="mutL"
                     /locus_tag="VFT03_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU4847638.1"
                     /translation="MSIRVLDPRVAQQVAAGEVVDRPASVVKELAENALDAGASRVEV
                     ELDDGGTSRILVRDDGSGMPPDDARLSVLRHATSKIRAVKDIEAVTTLGFRGEALPSI
                     ASVSSFTLTTSTGEGSGTRVVIEGGAEAEVSPASHPKGTTVVVDRLFYNVPARRAFLR
                     GPRAERAAISEVLTHLSIAHPNVAFRLTEKGRDYLSLPAAGDLLERLAQILGVAKARA
                     MRKVEYESGAFAVSGYAALPSLTEGSRAHQTISVNGRFVRAENLHRGLDDAYRATVPG
                     GRYPPVALEISVDHRRVDVNVHPTKQVVRFSEERAARDAVAAAVRNAIEWRAPATVTA
                     PRPSERTFTQERLSPRPSYAPREQASRTAEDRPAYPARDLREARERLAEASRPLAEER
                     GTYGELPERGALPALEDLRVIGQLAAGYILVEEPDEAMWVVDQHVAHERAILDRLHDS
                     DSPATMQSLLVPEVVELSPSEAEIAAASLDELSVYGFEAEPFGPRSIRVRAVISTLAD
                     RDIAGAFKDALSAITGTDPGHNREDRILATIACHSAVKMGDRLSQPEMEALIKDWLTS
                     RLPATCPHGRSICFRLGLHEVALKLDRH"
BASE COUNT          377 a          857 c          711 g          354 t
ORIGIN      
        1 gttgcgccgc acctctgggg gcgccagttg ccgcagcgct ctcgtccctg gccttgaccg
       61 agatctcgac gtcaccccgg cggacccgga tctcgcctac cccgctccgg cgcacgaggt
      121 ccacgagccg ctcgacttcc tcgaacggca gcggttctcg acgcgtctcg tcctgctgtg
      181 tctgatccac gtcctgcata gcctgggatt ctacacgtcc gaccgtggga aagtgcgccc
      241 ccgcatcttc aatgaggatc tcccttctac gcactcgggt gaatagagtc gttcggacta
      301 gccatcggcc agggtcttgg accagccgat ctgcgccaac agctcgagta aaccccaata
      361 ctgacgctcg gctacaaagt gcccgccgcg cctgtaaaat cgccacttgt gggtcttgcc
      421 gacccatagg agaatcaatg ccataagctt ccatgatcga ggtcccatga gcatccgagt
      481 ccttgacccc cgtgtcgccc agcaggtcgc cgccggggag gtcgtcgacc gcccggcctc
      541 cgtagtgaag gagctcgcgg agaacgccct cgacgccggc gcctcccgcg tcgaggtgga
      601 gctcgacgac ggcgggacct cccgcatcct ggtgcgcgac gacggctccg gcatgccgcc
      661 ggacgacgcc cgcctctcgg tcctgcgcca cgcgacgagc aagatccggg ccgtcaaaga
      721 catcgaggcc gttaccactt tgggcttccg cggcgaggct ctgccctcca tagcgagcgt
      781 ctcctccttc accctcacca cctcgaccgg cgaagggagc gggacgcgcg tcgtcatcga
      841 aggcggcgct gaggccgagg tctcccccgc ctcgcacccg aaagggacca ccgtcgtcgt
      901 tgaccgtctt ttctacaacg tcccggcaag gcgggccttc ctgaggggcc cgcgggcaga
      961 gcgggcggcg atctccgagg tcctcaccca cctctccatc gcccacccga acgtcgcctt
     1021 ccgcctcacg gagaagggca gggactatct atctctgccc gccgccggtg acctcctgga
     1081 gcggctcgcc cagatattag gcgtggcgaa ggcacgggcc atgcgcaagg tcgagtacga
     1141 gtcgggggcg ttcgctgtct ccggctacgc cgccctcccg agcctaactg agggtagccg
     1201 cgcccaccag acgatctccg tgaacggccg cttcgtgagg gcggagaacc tccaccgcgg
     1261 cctcgacgac gcctacaggg cgaccgtgcc gggcggccgc tacccgcccg tggctctcga
     1321 gatcagcgtc gaccaccgca gggtggacgt gaacgtccac cccacgaaac aggtggtgcg
     1381 cttctccgag gagcgggccg cccgcgacgc agtcgccgca gccgtccgca acgccatcga
     1441 gtggcgcgct ccggccacag taaccgcccc gcgcccttca gaacggacct tcacccagga
     1501 acgcctctcg ccgcgacctt cgtacgcgcc gcgggagcaa gcctcccgca ccgccgagga
     1561 ccggcccgcc tatcccgccc gggacctgcg cgaggctcgc gagcgcctcg ccgaggcctc
     1621 gcgcccgctc gccgaggagc gcggtacgta cggggagttg ccggagcgcg gcgcgctgcc
     1681 ggcgctcgag gacctgcggg ttataggcca gctcgcggcg ggctacatcc tcgtggagga
     1741 gcccgatgaa gccatgtggg tcgtggacca gcacgtcgcc cacgagcggg cgatactgga
     1801 ccggctccac gactccgaca gccccgccac tatgcagagc ctgctggttc ccgaggtggt
     1861 cgagctctcg ccctcggagg cggagatcgc cgccgcgagc ctcgacgagc tctccgtcta
     1921 cggcttcgag gcagagccct tcggaccgag atctatccgc gtgagggcgg taatctctac
     1981 gctggcggac agagacatcg cgggcgcctt caaggacgcc ctctcggcaa taaccggcac
     2041 cgacccgggc cacaacaggg aagatcgcat cctggcgacc atagcttgcc actcggccgt
     2101 caagatgggc gacagacttt cccagcccga gatggaggcc ctcattaaag actggctcac
     2161 cagccgcctc cccgccacct gcccgcacgg acgctcgatc tgcttccgcc tcggactgca
     2221 cgaggtcgcc ctcaagctag accggcacta ggatgagcaa tttcctgttc ctgggggccg
     2281 tctcctgcgc cctagccag
//
LOCUS       DASTUV010000276         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1072953, whole genome shotgun sequence.
ACCESSION   DASTUV010000276 DASTUV010000000
VERSION     DASTUV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072953"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /gene="fusA"
                     /locus_tag="VFT03_05530"
     CDS             <1..2002
                     /gene="fusA"
                     /locus_tag="VFT03_05530"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4847639.1"
                     /translation="HRGTGKTTIAETMLGLASGRSGPAGHHALDYTEEESERGMTLGM
                     NVATLEWKGRQVNVLDTPGDGGFVADAFVAQRVADCAILVVHAQDPIQVVTERVWRRG
                     EREGIPHVIVVNHLDRERTDFGAVVEQLRARFGQAVVPLNLPIGREHDLKGVYGLLTG
                     TAFVDGEQTEEIPEGMEDEVDEAKIQLFEAIAESDDTLLEKYLEGEELSTEEMFEGIR
                     KGIADGIIIPVLAASAERNIGMDRILDVIAGSAPSPVDRSRWIDEKGDDVPCDPEGPF
                     AAYVFKTYQDPYAGRLSVLRVVSGRCRSDEALTNPRTGSSERLGGIAHLVGKERLPVD
                     EAVAGDIIAVAKLKDTNTFDTLSKPDRQMLFEPVELPEPTSAFAVGARARGEEEKVFD
                     AIRRVVDEDPSLKLERSEATGEDILAGLAQMHVELALERISRRYGVEVASRAPKVPFK
                     ETITGSAQAQGRYKKQTGGRGQFGDARIEVSPLPRGEGFEFEDGIVGGAIPRQFIPAV
                     EKGIVESMQQGSIAGYPVVDVKVRLYDGAFHSVDSSEMAFKVAGSMAFKNAVEQAHPV
                     LLEPYMKVEVLAPTEMVGDIMGDLSGRRGRPMGIEQRGERQVIQAEVPQIEMLTYARD
                     LRSITGGRANFHVESGHYEEVPPNLVDRVLAANDREEEKAVS"
BASE COUNT          419 a          669 c          745 g          306 t
ORIGIN      
        1 ccacaggggc accggaaaga ccaccatcgc cgagacgatg ctcgggctgg catcggggag
       61 gagcggccct gcggggcacc acgctctcga ctacaccgag gaggagtccg agcgcgggat
      121 gacgctcggg atgaacgtag ccaccctcga gtggaagggg cgccaggtca atgtgctcga
      181 cacccctggc gacggggggt tcgtcgctga cgccttcgtc gcacagaggg tggccgactg
      241 cgcgatcctg gtagtgcacg cccaggaccc gatccaggtc gtcaccgaac gcgtgtggag
      301 gcgcggggag agggagggca tcccgcacgt catcgtcgtc aaccacctgg acagggagcg
      361 cacggacttc ggcgccgtcg tcgagcaact ccgcgccagg ttcgggcagg cggtcgtgcc
      421 gctcaattta cccataggcc gcgagcacga cctcaaggga gtctacgggc tcctgaccgg
      481 gacggccttc gttgacgggg agcagactga agagatcccc gaaggcatgg aggacgaggt
      541 agacgaggcc aagattcagc tcttcgaggc tatcgcagag agcgacgaca cgcttctcga
      601 gaagtacctc gaaggcgagg agctctcgac cgaggagatg ttcgagggga taaggaaagg
      661 gatagccgac ggcatcatca tcccggtcct cgccgcgagc gcggagcgca acatcgggat
      721 ggaccggatc ctggacgtca tcgccggcag cgccccgagc cccgtggacc gctcgcgctg
      781 gatagacgag aagggagacg atgttccctg cgacccggaa ggacccttcg ccgcctacgt
      841 gttcaagacc taccaggacc cttacgcggg acgcctgagc gtcttgaggg tcgtgagcgg
      901 caggtgccgt tcggacgagg cgctcaccaa tccgcgcacg ggctcctcag agaggctcgg
      961 cggcatcgct cacctcgtgg gcaaggagcg tttaccggtc gacgaggcgg tcgcgggcga
     1021 catcatagcg gtggccaagc tcaaggacac caacaccttc gacactctgt ccaagccgga
     1081 taggcagatg ctattcgagc cggtagagtt gccggagcct acctcggcct tcgcggtcgg
     1141 ggcgagggcg cgcggcgagg aggagaaggt cttcgacgcg atacggcgcg tggtggacga
     1201 ggacccctcg ctcaagctcg agcgctccga agcgaccgga gaggacatcc tcgcagggct
     1261 ggcgcagatg cacgtcgagc tcgcgctgga gcgcataagc aggcgctacg gggtcgaggt
     1321 cgcctcgagg gcgcccaagg tcccgttcaa ggagacgatc accggctcag ctcaggccca
     1381 gggccgctac aagaagcaga ccggcgggcg cgggcagttc ggggacgcca ggatcgaggt
     1441 ctccccgctc ccacgcggcg agggcttcga gttcgaggac ggcatagtcg gaggggcgat
     1501 cccgcgccag ttcatccccg cggtcgagaa gggaatagtg gagtcgatgc agcagggctc
     1561 gatcgccggc taccccgtcg tcgacgtaaa agttcgtctc tacgacggcg cattccactc
     1621 tgtggactcc tcggagatgg cgttcaaggt cgcagggtcg atggcgttca agaacgccgt
     1681 cgagcaggcc caccccgtcc tcctcgagcc gtacatgaag gtggaggtgc tcgcgccgac
     1741 ggagatggtc ggggacatca tgggcgacct ctcgggcagg cgcgggcgcc ccatgggcat
     1801 cgagcagcgc ggcgagcgcc aggtcatcca ggccgaggtg ccccagatcg agatgctcac
     1861 ctacgcccgc gacctgcgat ccatcacagg cggccgcgcc aacttccacg tcgagtcggg
     1921 ccactacgaa gaagtcccgc cgaacctggt ggacagggtc ctcgccgcca acgataggga
     1981 ggaagagaaa gcggtcagct aattagcacg cggcccggct tcgccaggca gcttgtcagc
     2041 acgccgcctt tggctccgcc aggcggcttc tcagcttctc tcggcacaaa aagagagagt
     2101 aagctgaaat gctgagaagc tagcctcccg cgtagctgg
//
LOCUS       DASTUV010000277         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_20599, whole genome shotgun sequence.
ACCESSION   DASTUV010000277 DASTUV010000000
VERSION     DASTUV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20599"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..881)
                     /locus_tag="VFT03_05535"
     CDS             complement(57..881)
                     /locus_tag="VFT03_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU4847640.1"
                     /translation="MDGRKIRAAAIQMSSTPDKEENKVTAEALIRGAVSAGADLVALP
                     ELWSCHGLDEVYRENAEPVPGPTTELMGDLARELGVYLLGGSILEGERGARRLSNTST
                     LFDPSGEMTAVYRKIHLFDVRVSDREYLESANIAPGNEVVTAKAGAATLGLSVCYDVR
                     FPELYRLLALRGAEILAVPAAFTLQTGKDHWELLLRARAVENQAFVVAPAQWGQKADG
                     RWTYGRSMIVDPWGTVLATCPDHDGHALATLDLDFLDRFRAEFPALANRRPEAYDW"
     gene            complement(931..1827)
                     /gene="ftcD"
                     /locus_tag="VFT03_05540"
     CDS             complement(931..1827)
                     /gene="ftcD"
                     /locus_tag="VFT03_05540"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEU4847641.1"
                     /translation="MKPPLFEAVPNFSEGRDEDRIARIVAAVRPVEDIRILGLHSDPD
                     HNRSVLTFAGEEAAVLEAAVALAEACAREIDLTSQTGEHPRMGSLDVLPFVPLEGATL
                     DEATRLARQVGERLGALGFLVYLYEAAATAPHRRNLADVRRSGYDGLAARLEDPLWQP
                     DYGPRELDPRKGAVAVGARPLLVAFNAYLDTDDVEVARAIALEVRERDGGLPGVKALG
                     LEVGGRAQVSMNLTDLQKTSMPLALEAVKAAALEHGASVESTELVGLAPLEAVLQTAR
                     HYLGLRELDREDVLDVALWDTP"
     gene            complement(1835..>2078)
                     /locus_tag="VFT03_05545"
     CDS             complement(1835..>2078)
                     /locus_tag="VFT03_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU4847642.1"
                     /translation="GDEGSFSLCSFWLVECLTQADRLDEARLALEKMFSYSNHLGLYA
                     EEIGAGGEALGNFPQALTHLSLISSAVHLDRALGAT"
BASE COUNT          342 a          682 c          694 g          360 t
ORIGIN      
        1 ctaaggcgtt tcgcgagagg tttcaggcgc cggcatactt acctgaggcc ttctggttac
       61 cagtcgtagg cttcggggcg gcggttagcg agcgctggga actcggcgcg gaagcggtcg
      121 aggaagtcaa ggtcgagagt cgccagggcg tgaccgtcat ggtcggggca cgtggcgagt
      181 accgttcccc aggggtcaac gatcatgctc cgcccgtacg tccagcgccc gtcggccttc
      241 tgcccccact gggctggcgc tacgacgaaa gcctggttct ctacggcccg agcgcgaagt
      301 agcagctccc agtggtcctt gccggtctgc agggtgaagg cagcggggac ggccagtatc
      361 tccgcgccgc gcaatgcgag gaggcggtag agctcgggga agcgcacgtc gtagcagacc
      421 gagagcccca gggttgcggc gccggccttg gcggtcacta cctcgttgcc cggcgcgatg
      481 ttcgcgctct ccaggtactc cctgtccgag actctcacgt cgaagaggtg gatcttgcgg
      541 tacaccgccg tcatctcccc tgagggatcg aataacgttg aggtgttgga caaccgccgc
      601 gcgccgcgct cgccctccag gatcgaaccg ccgagcaggt acacgccgag ctcgcgagcc
      661 aggtccccca taagctccgt cgtcggaccg gggaccggct ctgcgttctc acgatagacc
      721 tcatcgaggc cgtggcagct ccagagctcc ggcagcgcca cgagatcggc gcccgcggag
      781 acggctcctc tgataagcgc ctccgccgtt accttgttct cctctttgtc aggcgtcgag
      841 gacatctgga tcgccgccgc cctgatcttc cgaccgtcca tcaagctcct ccttctagca
      901 cagcctcaga ctagcacgcc tgaccgctcg ttacggtgtg tcccagagcg caacatccag
      961 gacgtcctct ctatcgagct cccgcagccc caggtagtgc cgggccgtct gcaagacggc
     1021 ttccagaggc gcgagcccga ctagctccgt ggactcaacc gatgccccgt gttcgagcgc
     1081 cgctgccttc acggcttcga gagctagtgg catcgaggtc ttctgaaggt ccgtcaggtt
     1141 catcgagacc tgcgccctgc cgccaacctc cagcccgagc gccttgacgc caggcaaccc
     1201 gccatcgcgc tcccgcacct ccagggcgat ggctctggcc acctctacgt cgtcggtgtc
     1261 gaggtaggcg ttgaaggcga ctaggagggg acgagcgccg acggctactg ctcccttgcg
     1321 ggggtcgagc tctcgcgggc cgtagtccgg ttgccagaga gggtcttcca ggcgcgcggc
     1381 gagaccgtcg tagccgctcc gccgcacgtc agcaagattg cgccggtgtg gggcggtggc
     1441 cgcggcttca tagaggtaga cgaggaaccc gagggccccg agcctctccc ccacctggcg
     1501 tgccaggcga gtggcctcat cgagcgtagc cccttccagc ggcacgaacg gcagcacgtc
     1561 gagcgagccc atcctcgggt gctcacctgt ctgcgaggtg agatctatct cccttgcgca
     1621 ggcttcggcc agcgcgacgg ctgcctccag gacggctgcc tcctcgccgg cgaaagtaag
     1681 gacgctgcgg ttgtggtcgg ggtcagagtg aaggccgagg atccggatgt cctctacggg
     1741 ccgaacggcg gcgacgatac gagcgattct gtcttcgtcg cgcccctcgc tgaagttggg
     1801 gactgcttcg aagagcggtg gtttcacgga ggatctaggt cgcgcccagg gcacggtcga
     1861 ggtggacggc ggagcttata agggagaggt gggtaagggc ctgggggaag ttgcccaggg
     1921 cctcgccgcc ggccccgatc tcctcggcgt agaggccgag atggttggag taagagaaca
     1981 tcttctccag agcgaggcgg gcctcgtcga gacggtcagc ctgtgtcaga cactcgacga
     2041 gccagaagga gcagagactg aagctgcctt catcgccc
//
LOCUS       DASTUV010000278         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_782847, whole genome shotgun sequence.
ACCESSION   DASTUV010000278 DASTUV010000000
VERSION     DASTUV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782847"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..2489
                     /locus_tag="VFT03_05550"
     CDS             495..2489
                     /locus_tag="VFT03_05550"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU4847643.1"
                     /translation="MAENLVTKILREHMVDGSLDPGEEVGLRIDQTLLQDATGTMACL
                     QFERMGEDRVRVPLALQYVDHNVIQLDFKNPDDHRFLQAFAAKYGILYSRPGNGICHY
                     LHVERFARPGATLIRADSHTTSSGALGSIAIGAGGLDVALVMAGQPFQTPAPKVVGVE
                     LTGTLSEWVTPKDVILELLRRRGVRGGLGRIFEFFGPGVASIDVTGRTTICNMIAELG
                     ATTGVFSSDERTREWLEMQQRPEDFVRLSADEGADYDEREQIELDALEPLIAQPHSPG
                     NVGPVSEVAGTKVAQVCIGSSVNSGFDDLAIAAAVLRGETVYPGLVMTVTPGSRQILD
                     AIARSGVYSDLVRAGARMLEPVCGPCVGMGQAPPSDSVSVRTFNRNFPGRSGTITDQV
                     YLTSPTTAAATALRGVITDPRELGEYPDLPEAPANPAVDDRQILDPAPAEEARGIEVP
                     RGPNIKPPPEAPELPGSLECTVTIVVGDDVSTGDLAPDGVEVMSFRSNVPEIARFTFR
                     RFDPEYYKKAKEMAPGFIVGGHNYGQGSSREHAALAPLHLGIRAVIAKSFARIHRRNL
                     ISQGILPLQFTDQSDYEKFEQGNKWELPDLRKRLENGDEEVPLRGRDEEVTLLAEFSP
                     RERSILLAGGILRQLREKEGAPASVSKGESAEPEHRGRDY"
BASE COUNT          607 a          936 c         1025 g          529 t
ORIGIN      
        1 cgcacctctt cggcatcctc gaccgcataa acggtttagg attactgctc ctgagcgtgc
       61 aggccctgcc agaagacgcc caccccagtg ctaaaggaaa tcgatcgcca agccattaac
      121 cgggacccta accacctcac cggcgaggct tattcacccg agtgcgtgga aggggtgttc
      181 tcagaagtcc gtgtgcgaga tcctggttag aatgcgccgc gggccgggta gctgctcgtc
      241 ttcgcccgct taggcgcgat ggcgctatca caatgtgttg cagcggtgca tagacttcca
      301 cgagggtaga tgctcacctt cgggtcgggg atcggctcgg gttctccgca ttatggatga
      361 accgctcgtc cgatcaaccg gcgcgacctg aagtttctgc ccagcggatc aaccgcagta
      421 gccgtattgg gtttgcgggt cgccgtgtag aatgtggtca gggcttcggt tagcgagggc
      481 ataggggtgc ggcgatggcg gagaatctgg tcaccaagat cctcagggaa cacatggtcg
      541 atgggtcgct cgatcccggc gaggaggtcg ggctaaggat agaccagacg ctgttgcagg
      601 atgcgaccgg cacgatggct tgcctgcagt tcgagcggat gggggaggac agggttcggg
      661 tgcccctggc actccagtat gtagaccaca acgtcattca gctggacttc aagaaccccg
      721 acgaccaccg cttcttgcag gccttcgccg caaaatacgg catcctctac tcccggcccg
      781 gtaacggcat ctgccactac ttgcatgtcg agcggttcgc gaggcccggc gcgacactca
      841 tcagggcgga ttcccacacc acttcctcgg gcgccctggg ctccatcgcc ataggggccg
      901 gcggactcga cgtggcgctg gtcatggccg gccagccctt ccagaccccg gcgccgaagg
      961 tcgtcggcgt agagctcaca ggcacgctct cggaatgggt gacgccgaag gacgtcatcc
     1021 tggagctgct gaggaggcga ggcgtcaggg gcggcctcgg gcgcatcttc gagtttttcg
     1081 gccccggggt ggcgagcata gacgtcacag gccgcaccac gatctgcaac atgatcgctg
     1141 agctcggcgc caccactggc gtcttctcct cagacgagag gaccagggag tggctcgaga
     1201 tgcagcagcg gcctgaagac ttcgtcaggc tgtccgccga cgagggagcg gactacgacg
     1261 agcgcgagca gatcgagctg gacgctctgg agcctctgat cgcgcaaccc cactctccgg
     1321 gcaacgtggg cccggtgagc gaggtcgccg gcacgaaggt cgctcaggtg tgcattggca
     1381 gctcggtcaa ctcgggcttc gacgacctgg cgatcgcggc ggcggtcttg cggggggaga
     1441 cggtctaccc gggcctcgtc atgaccgtga cgccgggaag ccgccagatc ctcgacgcta
     1501 tcgcccggag cggcgtatac tcggacctcg tgcgggcagg cgccaggatg cttgaaccgg
     1561 tgtgcggccc gtgcgttggg atgggccagg cgcccccctc cgactcggtg agcgtcagga
     1621 ccttcaaccg caacttcccc gggcgctccg gcacgatcac cgatcaggtg tacctcacga
     1681 gcccgaccac cgcggcggcg acggcgctcc ggggggtcat caccgacccg cgcgagctgg
     1741 gcgagtaccc ggacctaccg gaggccccgg ccaacccagc ggtggacgac cgccagatcc
     1801 tagaccccgc gccggcggag gaggctcgcg gcatcgaggt accgcgcggc cccaacataa
     1861 agccgccacc cgaggccccg gagctgccgg gctcactcga gtgcaccgtg accatcgtcg
     1921 tcggggacga cgtctcgact ggagacctcg cgccagatgg ggtggaggta atgtccttcc
     1981 gctcgaacgt gcccgagatc gcccgtttca ccttccgccg cttcgaccct gagtactaca
     2041 agaaggccaa ggagatggcg ccgggcttta tcgtgggcgg gcacaactac ggtcagggta
     2101 gtagccgcga gcacgcggcc ttggccccgc tacacctcgg catccgagcg gtgatagcta
     2161 agagcttcgc ccgcatccac cggcggaatc tgatctccca gggcattctc cccctgcaat
     2221 tcacagacca atcggactac gagaagttcg agcagggcaa caagtgggaa cttccggacc
     2281 tgcgcaaacg cctggagaac ggtgacgagg aggtcccact gaggggacga gacgaagagg
     2341 taacactcct cgccgagttc tcgccacggg agaggagcat tctgctcgcg ggcggcatac
     2401 tgcgccaact tcgcgagaag gagggtgcgc cggcgtcggt aagcaagggc gagtccgcgg
     2461 agccggagca tcgcgggagg gactactaga tccaacggct tccagcttcg gtgcgagata
     2521 acctgcctcc ggtgaagggc acaattgcac tttaggattg tttacgcggc aatcgagggc
     2581 aacgatgagt ctttgcggct gacgtgcgac gacacagagg agatggaaga gacctctgtc
     2641 ataaggccta gacaggcgcg ctgtagccac gctgtagcca cagtgcatga agaagccgtg
     2701 agcgtcctga gtgttggagg agtgctggag taggacgatg gagctgcata agctcgacgt
     2761 agtagtcgca gggcattatt cacccgagtg cgtggaagtg gaattctgcg aacttcgccc
     2821 aaacggagtt ctcggaagta cgtatccagc atgttgaata gatccggccg gttagcggtg
     2881 ccgaaggctt ctgcttcttg gcgcatcgtg ctttccgcat actggttcaa tcgccatgaa
     2941 acaggggata tgtacaaacg acgaaaggtg gggttatgga ggactcgaag gtaatggcac
     3001 gggtgatctg caacccggcg tccagcggag ggggctacga tcccgaagag ctgcccagag
     3061 agctagaggg ctacgaccta gagtggatca ctaccga
//
LOCUS       DASTUV010000279         5925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_834361, whole genome shotgun sequence.
ACCESSION   DASTUV010000279 DASTUV010000000
VERSION     DASTUV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834361"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1275)
                     /locus_tag="VFT03_05555"
     CDS             complement(151..1275)
                     /locus_tag="VFT03_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU4847644.1"
                     /translation="MLCLALAAACSGASQENADVEARPQASSAGVQRERLAEVERMSV
                     RSLVGQMFMISMGSTEPDYYVEKMIRERNIGGVLLFGYNMESEAQTRALTGALQRLSM
                     RTEPAIPLFIAVDQEGGEVSSAPWVLPQPAAARVGARGDPGQAREIAEQMGGELLRAG
                     VNTDLAPVVDTGSGAAIGTRSYGEDPRLVAKMGAAAVEGFESAGVVSAAKHFPNHGPA
                     TTDSHASLPVVDHDRETLRSYDLPPFEAAVKAGVPMVMVGHLLYPAIDAHRPASLSPE
                     AVKLLRGELGFEGVVVTDDLAMAGASGGRSPARAAVEAVEAGADLLIISSPPQQQANA
                     YDAVVEAVESGEISRERVESSVGRLLQVKEKYSLDKAGVR"
     gene            1390..2751
                     /locus_tag="VFT03_05560"
     CDS             1390..2751
                     /locus_tag="VFT03_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU4847645.1"
                     /translation="MIRLLYESGTIVASGASGQDLPGSFVWDARTRQWRAPASAYREA
                     ILSLREAGTPHEDLASSFEKLSLESRVALAPRPYQREALAAWRRGELRGVVVLPTGAG
                     KTAVAIQGIERVGRSALVVVPTLALLKQWYSVLFDSFGASVTVGLLGGGYHEVTPLTV
                     TTYDSAYIHAERYGDRFALVVYDEVHHLPAPKNAVISKMLIAPYSLGLTATPERPDGG
                     HELLPELAGPVVYRRSPEDLAGVYLAPFELVRIPVELTAHERFEYAQADAVYRDFLAK
                     HRLPMRSPEDWQRFILVAATSHSGGREALLAARRRREIQEGAMRKVATLETLLRRHWD
                     DRVIVFAKSVEEVYSLSRRFLIPGVTYETPARERKEILDRFREGRYRAIIASDVLNEG
                     VDVPDAGVAIILAGSASRREYVQRLGRILRPREGKRATLYELVTAETGEVHAARRRRE
                     ALL"
     gene            2751..4025
                     /locus_tag="VFT03_05565"
     CDS             2751..4025
                     /locus_tag="VFT03_05565"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEU4847646.1"
                     /translation="MLRSEHVIARLSRGRILPYRLAHDDPHVLEVAHELCEAYAGHVG
                     GPRSHLEEALATREEELGPRLDARRGFRIVRGLSKLLEERAAWASPTAADPYTIRTRV
                     FELAAALPEPPAEEAGLLGAPTRDDVLSRVAAETGMDDPATLMYADRQGAQILEDFDE
                     PPPEELIARYNVAQVQGVLYAARDLVVDLREGADARLVFHYVKLQGLIYALEPVPRGY
                     RLRLDGPLSIFGATRKYGLRLARFLPGLLLTSPWRLTATVEWRDREAYLELDSETSGL
                     RSHYLGPRSEQEADPVREAFVRAWERAKDTGGWELEPGAGILSFPDRKAALVPDFTLR
                     HTGTGERVHLEVLGFWSERNLVERAALLREAGERGHRVLVAVSEKLGASPEALSEATK
                     TGVITFKERLATKAVLAALGEPGEASSGADPI"
     gene            4033..4485
                     /locus_tag="VFT03_05570"
     CDS             4033..4485
                     /locus_tag="VFT03_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847647.1"
                     /translation="MERSLGYLVALTVGCTFAAALFGFGAQVFAWKSAYEGDFGREQL
                     ILLTRLLVFLVLAVTLVLRGGWRGVLAAVAMVVGATLLEWALFPLAQEWAAISDPEGY
                     ERVVGEEVSRPTYGQWATYDIIGVAISAALAQGLRIMAHVNPRAPRGG"
     gene            complement(4457..4726)
                     /locus_tag="VFT03_05575"
     CDS             complement(4457..4726)
                     /locus_tag="VFT03_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847648.1"
                     /translation="MRTVTIRYTTFRECPNCEGRGDDGSSWRPFVCRRCQGRGRLVTG
                     VEVEDSVEGVLPRSRYDFNSLGEVYLLDFDEAEEIEVIRPAVPWG"
     gene            complement(4723..5754)
                     /locus_tag="VFT03_05580"
     CDS             complement(4723..5754)
                     /locus_tag="VFT03_05580"
                     /inference="COORDINATES: protein motif:HMM:NF039924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5937 family protein"
                     /protein_id="HEU4847649.1"
                     /translation="MIRIHVSAEDLTQTRFALSPLWETVESYRAILDPGRHALHLPWI
                     SWAREEIGGWELGPLDALVRARGYMPDFLTPPPTTPFPEFLTEVELLRATPPERVREE
                     VKRVAGEDTPALRAYLKDPEGSVERLAELLVLYHRRVLAPHWPRLHTLLEADVFKRAR
                     TLAFHGPEALFGGLHRSVGYRSGVVELYKDFEAEVRPGGRGVLLIPVAFAWPDVHVII
                     DAPWQPTLVYSPRGVARLWETGRPPTDEALTAALGAGRASVLKSLVVPSTTTELARSL
                     AISPSAVSQQLARLRRAGLVEPHRRGRRVYYRLSLCGEQLLDLFGELDDEAPPDRRLA
                     PFSAGSIGP"
BASE COUNT          934 a         2130 c         1928 g          933 t
ORIGIN      
        1 tagatcttag tcacgttttc catcgtgact tcagccatca ccgtctcctt cccttcatcc
       61 ggctatgtcc ggcgtaccgt ttatcccctg tttgcagatc acttccccca gtgattagag
      121 ccattctacc ccaactccag acttcgcaac ctagcgcacg cccgccttgt caagcgagta
      181 cttctctttg acctggagca atcgcccgac ggaggattcc acccgctccc tcgatatctc
      241 ccctgactcg acggcctcga cgacggcatc gtaagcgttg gcctgctgct ggggcggact
      301 ggagattatg agcaggtccg ccccggcctc taccgcctct accgccgccc gcgcaggcga
      361 tcggccgccc gacgcacctg ccattgccag gtcgtccgtg accaccaccc cttcgaaacc
      421 gagctccccg cgcagcaact ttacggcctc gggcgagagg cttgccggcc tgtgtgcgtc
      481 tatggccggg tagagcaggt gcccgaccat caccattggc accccggcct ttaccgccgc
      541 ctcgaaaggc ggcaggtcgt aggaacgcag cgtctcgcgg tcgtggtcca cgacgggcag
      601 gctcgcgtga gagtcggtgg tcgccgggcc gtggttcggg aagtgcttgg ccgccgagac
      661 gacgcccgcg ctctcgaagc cctcgacggc cgcggcgccc atcttcgcga ccagccgcgg
      721 gtcttccccg tacgagcggg taccgatcgc ggcgccggag cccgtgtcca ctacgggagc
      781 gaggtccgtg ttgacccccg cccgcaggag ctcgccgccc atctgctcgg cgatctcgcg
      841 cgcctgcccg gggtccccgc gcgcgccgac ccgcgcggct gccggctgcg gaaggaccca
      901 gggcgcggag gagacctcac ccccctcctg gtccacggct atgaagaggg gtatggcggg
      961 ctccgtcctc atggatagcc gctgcagcgc gccggtgagc gccctcgtct gcgcctcgct
     1021 ctccatgttg tagccgaaga gcagcacgcc cccgatgttc cgctcccgga tcatcttctc
     1081 gacgtagtag tccggctcgg tggagcccat gctgatcatg aacatctgcc ccaccaggct
     1141 gcgcacgctc atcctctcga cctccgccag tcgctctcgc tgcaccccgg ctgaagaagc
     1201 ctgcgggcgc gcctcgacgt cggcgttctc ctgcgaagcc ccggagcacg ccgcagccag
     1261 cgccaggcag agcacgccga ggacgcttac cagccgggtt cttttcgcga agcctcgcag
     1321 atccctcctc ctggccgccg cgaccagttt accgctgagg agagcgggaa tctcgggtat
     1381 ccttgcggcg tgatccggct tctctacgag tcaggcacca tcgtcgcgtc cggggcctcg
     1441 ggccaggatc tccccgggtc cttcgtctgg gacgcccgca cccggcagtg gcgcgccccg
     1501 gcgagcgcct accgcgaggc gatactgagc ctgcgcgagg cggggacgcc acacgaagac
     1561 ctggcctcga gcttcgagaa gctctcgctc gagtccaggg tcgcgctcgc gccccgccct
     1621 taccagaggg aggccctcgc cgcctggcgc aggggagagc tgcgcggcgt cgtggtcctg
     1681 ccgaccggag cggggaagac cgcggtcgcc atacagggga tagagcgcgt cgggcgcagc
     1741 gcgctcgtag tcgtcccgac gctcgcgctg ctcaagcaat ggtactcggt cctgttcgac
     1801 tccttcggag cctcggtcac ggtcggcctg ctgggcggcg gctatcacga agtcacgcct
     1861 ctcaccgtca cgacatacga ctctgcctac atccacgccg agcgctacgg cgaccgcttc
     1921 gcgctcgtgg tctacgacga ggtccaccac ctccccgcgc ccaagaacgc cgtcatctcg
     1981 aagatgctca tcgcccccta ctctcttggg ctgaccgcca ccccggagcg ccctgacggc
     2041 ggccacgagc tactccccga gctggccgga cccgtcgtct accggcgctc gcccgaggac
     2101 ctcgcggggg tctacctcgc gcccttcgag ctggtgcgca tccccgtcga gctcacagcc
     2161 cacgagcgct tcgagtacgc ccaggcagac gccgtataca gggacttcct cgccaagcac
     2221 cgtctcccga tgcgctcgcc ggaggactgg caacgcttta tcctcgtcgc cgcgacgagc
     2281 cactcgggag ggcgagaggc gctactggct gcgcggcgca ggcgagagat ccaggagggc
     2341 gccatgcgga aggtggcgac cctcgagacc ctgctgcgcc gccactggga cgaccgcgta
     2401 atagtcttcg ccaagagcgt cgaggaagtg tactcgctaa gccggagatt cctgatcccc
     2461 ggcgtcacct acgagacccc ggccagagag cggaaggaga tcctggaccg cttccgggag
     2521 ggccgctacc gcgccatcat cgcctcagac gtcctgaacg agggcgtcga cgtccccgac
     2581 gcgggcgttg cgatcatcct cgccggcagc gcctcccgcc gcgagtacgt tcaaaggttg
     2641 ggccgcatcc tccgcccgcg cgagggcaaa cgggccaccc tctacgagct cgtcacggct
     2701 gagacgggcg aggtgcacgc cgcgaggcgc cgcagggaag ccctcctcta gtgctccgta
     2761 gcgaacacgt gatcgcgcgc ctcagccgcg gcaggatcct tccctaccgg ctcgcgcacg
     2821 acgacccgca cgtccttgag gtggcacacg agctctgcga agcttacgcc ggacacgttg
     2881 gcggcccgag atcacacctc gaagaggccc tggcgacccg cgaggaggag ctgggtcctc
     2941 ggctcgacgc ccgccgggga ttcaggatcg tgcgcgggct ctccaagcta ctcgaggagc
     3001 gtgcggcgtg ggcctccccc accgcggccg acccgtacac tatacgcacc cgcgtcttcg
     3061 agctcgccgc cgccctgccg gagccgccgg ccgaggaggc cggcctgctc ggggccccta
     3121 cccgcgacga cgtgctgtcc cgggtcgccg cggagaccgg gatggatgac ccggccacgc
     3181 tcatgtacgc agacaggcag ggagcccaga tcctggagga cttcgatgag cctcccccgg
     3241 aagagctcat agcccgctac aacgtagccc aggtgcaggg cgtgctctac gccgcccgcg
     3301 acctcgtcgt ggacttgcgt gaaggggccg acgccaggct cgtcttccac tacgtcaagc
     3361 tgcaaggtct gatctacgcg ctcgagcccg tcccccgcgg ctaccggctc cgcctcgacg
     3421 gcccgctctc gatcttcggc gcaacccgca agtacgggct caggctggcc aggttcttgc
     3481 cgggcctcct cctcacctca ccgtggcgcc tcacggcgac agtcgagtgg agggaccggg
     3541 aggcttacct cgagctcgac tcggagacga gcggcctccg gagccactac ctcggacccc
     3601 gcagcgagca agaggccgac cccgtgcgcg aagccttcgt ccgcgcgtgg gagcgcgcga
     3661 aggacaccgg cggctgggag ctggaaccgg gggccggtat cctctccttc ccggatagaa
     3721 aggccgcgct agtgccggac ttcaccttga ggcataccgg gaccggggag cgagtccacc
     3781 tggaggtgct cggcttctgg tccgagcgca acctcgtgga gcgggccgcg ctgctgcggg
     3841 aagccggcga gcggggccac cgcgtgctgg tcgccgtctc ggaaaagctc ggcgcttccc
     3901 ccgaggcgct ctccgaggcc accaagaccg gtgtgatcac cttcaaggaa cgcctggcga
     3961 cgaaggccgt gctcgcggcg ctcggggagc ctggggaagc ttcgtcaggc gcggacccaa
     4021 tataatcggg gaatggagcg gagccttgga tacctcgtcg cgctcacggt gggttgcacc
     4081 ttcgccgccg ctctcttcgg gtttggcgcc caggtcttcg cctggaagag cgcctacgag
     4141 ggcgacttcg ggcgggaaca gctgatcctg ctcacccgtc tactcgtctt cctggtgctg
     4201 gccgtcaccc tggtcctccg gggcggctgg cggggggtgc ttgcggcggt cgcgatggtc
     4261 gtcggagcca cgctcctgga gtgggcgctc ttcccgctgg cccaagagtg ggcagccata
     4321 agcgacccgg agggctacga gcgggtggtc ggcgaggagg tcagcaggcc cacctacggg
     4381 cagtgggcca catacgacat aataggcgtc gcgatctcag ctgccctcgc gcaggggttg
     4441 cgaatcatgg cgcacgtcaa ccccagggca ccgcggggcg gatgacctcg atctcctccg
     4501 cttcgtcgaa gtccagcaag tacacctccc ccagagagtt gaaatcgtag cgcgagcgcg
     4561 gcaagacacc ctccaccgaa tcctcgacct cgacgccggt tacgagcctg cctcgcccct
     4621 gacacctccg gcacacgaag ggccgccagg aggagccgtc gtccccgcgc ccctcgcagt
     4681 tggggcactc cctgaacgtc gtgtagcgga tcgtcacggt tctcatggcc cgatactacc
     4741 agcagagaac ggggccagcc gtcgatccgg cggggcctcg tcgtccagtt cgccgaagag
     4801 gtcgagcagt tgctccccgc acaagctcag gcggtagtag accctcctcc ccctgcggtg
     4861 aggctccacc agcccggcgc ggcgcaaccg cgccagttgc tgagagaccg cgctggggct
     4921 gatcgcgagc gacctggcca gctcggtggt ggttgacggc accaccaggc tcttgagcac
     4981 cgaagcgcgc cctgcaccga gcgcagccgt aagggcctcg tccgtggggg ggcgacccgt
     5041 ctcccagagc ctcgccaccc cgcgcgggct gtaaacgagc gtcggctgcc acggcgcatc
     5101 gatgatcacg tggacgtccg gccaggcgaa ggccacaggg atcaggagca cccccctgcc
     5161 tcctggcctc acctcggcct cgaagtcctt gtagagctcg acgaccccgc tgcggtagcc
     5221 tacggaacgg tgcaacccgc cgaagagtgc ctcgggcccg tggaaggcga gcgtacgagc
     5281 gcgcttgaac acgtcggcct cgagcagggt gtgcagccgg ggccagtgcg gggccagaac
     5341 gcgtctgtgg tacaggacga gtaactccgc caggcgctcc acggagccct cgggatcttt
     5401 caggtaggcc cggagcgccg gggtatcctc gccggcaaca cgcttcacct cttctcgcac
     5461 cctctcgggc ggggtggccc gcagcagctc cacctcggtg aggaactccg ggaatggcgt
     5521 ggtcggaggc ggggtgagga agtcgggcat gtagccccgc gctcgcacca gggcgtcgag
     5581 ggggccgagt tcccaccccc ctatctcctc ccgcgcccag gagatccagg gcaggtgcag
     5641 cgcgtgccgg ccagggtcga ggatcgcccg gtagctctca accgtctccc aaagcgggct
     5701 gagggcaaag cgggtctgcg tgagatcttc ggcggagacg tggatccgga tcattcgcgg
     5761 agatcctcct tcttctcgac cgcccacgct attgtagcca cagtttagaa aaaaccgaat
     5821 gttttccctt acggcggggg gcagcgtact ctcagatcag agtgcaggat ctcgggaggt
     5881 gaaccggaga tgtacgggtg gttggactat cgggctcgcc atcac
//
LOCUS       DASTUV010000280         8369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_643820, whole genome shotgun sequence.
ACCESSION   DASTUV010000280 DASTUV010000000
VERSION     DASTUV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643820"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFT03_05585"
     CDS             complement(<1..386)
                     /locus_tag="VFT03_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847650.1"
                     /translation="MEPEHRTSDGEGRAPSRSGDLRARGSTAKREVASWVNWQSALYL
                     IASGWICVVLAPLTSFWWLAMVFASVVPIALSVLDRPGLETSDERKVKEGELLHTLAE
                     RREITPATAAMWTSLTVDEASTMLDEL"
     gene            complement(509..1576)
                     /locus_tag="VFT03_05590"
     CDS             complement(509..1576)
                     /locus_tag="VFT03_05590"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU4847651.1"
                     /translation="MTNIPVLDGHNDTLLKLRFPERGEERNFFERSDVGHIDLPRARE
                     GGFGGGFFACYVRNPENDGWTEESALTLTKTGYEVADAPPLDPVHARRKADELVGVLF
                     DLEAESEDKLEVVRTAREIESCLSEGVLAAILHFEGAENLGPDPGALEDLYEVGLRSL
                     GLVWSRPNAYAHGVPFRFPSPPDTGPGLTDAGKELVRVCNRLGVLLDLSHLNERGFWD
                     VASITETPLVATHSNAHALCPTSRNLTDRQLDAIRDSDGMVGVNFAVAFLREDGAEEE
                     DTPIEDVVRHVDYLVERVGVERVGFGSDFDGAKVPLGIRDASGLPNLLAELRERGYDD
                     AALKMLAHENWVRVLRETWGQ"
     gene            complement(1666..2850)
                     /gene="moeB"
                     /locus_tag="VFT03_05595"
     CDS             complement(1666..2850)
                     /gene="moeB"
                     /locus_tag="VFT03_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU4847652.1"
                     /translation="MQTRKPLVESPNGSVDLSNEEIARYSRHLIMPEVALEGQKQLKQ
                     ARVLTIGAGGLGSPLAMYLAAAGIGTIGVVDFDVVDESNLQRQIIHGTSDLGRPKLES
                     ARETIEDINPNVNVETFGEPLSSENALEIFEDFDVIVDGTDNFPTRYLVNDASVLTGK
                     PNVYGSIFRFEGQASVFYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGAIGTIQ
                     ATETVKLILGIGEPLIGRLLLYDALGMRFREMKLRKDPNCPVCGENPTVTELIDYQEF
                     CGIPQANAQAQADEVPEITVQELEGRLQNGSKVSVLDVREPHEYEVANIGARLIPLAE
                     LPERLVELDRDETLAVHCKSGGRSARAVKLLKEAGFQNVYNVRGGIDAWSEEIDSSVP
                     KY"
     gene            complement(2908..3201)
                     /locus_tag="VFT03_05600"
     CDS             complement(2908..3201)
                     /locus_tag="VFT03_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEU4847653.1"
                     /translation="MPKIKIPTPLRQYTGGDSEVEVGGETAGETLGNLASEYPDLRQH
                     LYTGDGKLRSFVNVYKGDEDIRYLDGADTEVSEGDELSIIPSIAGGCAGRRCR"
     gene            complement(3221..3640)
                     /locus_tag="VFT03_05605"
     CDS             complement(3221..3640)
                     /locus_tag="VFT03_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEU4847654.1"
                     /translation="MPLKIGRGDVDHIHEHARESYPEECAGAMVGMNLDGMKIVVDVW
                     RAENTHEDERSRRFLIEPLKIKEFEERAAERDMDVLGFYHSHPDHPAEPSRYDREHAW
                     PFYSYVIASVGENGVEDLRSWVLRDDRSGYEEEPIVS"
     gene            complement(3647..4570)
                     /locus_tag="VFT03_05610"
     CDS             complement(3647..4570)
                     /locus_tag="VFT03_05610"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEU4847655.1"
                     /translation="MITRINEPTGTKVTELVGNTPLLEFPSISADVPGVRILGKAEWY
                     NPGGSVKDRPALWMIRDGEKSGALTPEKTILDATSGNTGIAYAWICAALGYKVRLCMP
                     SNASQERKKILRAYGVEFILTDPGEGSDGAIREARRLYAEEPEKYFYPDQYSNPANPR
                     SHYESTAPEIWEQTGGEITHFVAGLGTSGTFVGTARRLKEYNPDIKVISFEPESPFHG
                     LEGMKHMESAIVPPIYDPSVADENRRASTEDAYEMVKRVAREEGVLIGISAGAAVATA
                     LEVAREIENGVVVTILCDGADKYLSERFWED"
     gene            complement(4741..5463)
                     /locus_tag="VFT03_05615"
     CDS             complement(4741..5463)
                     /locus_tag="VFT03_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU4847656.1"
                     /translation="MSETRTQQQAAQEESLPAQYVNYVFFKLDPLWRRMGDEERERGK
                     KEFLEAIEEHSQDMLLRSFSLMGLRSDADFMLWRIGYDLDAFEKMTSALMKTGLGRYL
                     TVTYSYFALSRRSIYVGEHTPGFENRRYIVPGEGDYLFVYPFVKTRAWYQLPLEERQR
                     MMDEHMKIGRKHYPVKNNTAYCFGIDDYEFILSFEAESPEKFQNLMMELRESEASSYT
                     ELDTPIFTCRRRTLPEILESLA"
     gene            complement(5586..6593)
                     /locus_tag="VFT03_05620"
     CDS             complement(5586..6593)
                     /locus_tag="VFT03_05620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4847657.1"
                     /translation="MRYRNLAGTDIRISEVGFGVWTVSTGWWGEVDDERSVRLLRRAF
                     EKGINYFDTADTYGSGKGETLLADAFGGMRDEVVISTKIGYDFYNHTARRGQQERPQD
                     WSEDFIRFALEQSLKRLGTDYIDFLQLHNTKMDAIENDRLFELMNEFVEEGKIRSYGV
                     ALGPKIGWLEEGEKAMRERDLAGVQMIYNLLEQDPGQSLIKTALETDTSLIVRVPHSS
                     GMLEGKYTEDTTFAKNDHRRHRSKEWLLDGLKKVEQLSFLTESGERTLGQAALKFILV
                     SPQIVSTLPNIYDDEQLDEFAAAPDTPDLSQDELERVAELYENNFGLEASDEKASGIR
                     H"
     gene            6794..7510
                     /locus_tag="VFT03_05625"
     CDS             6794..7510
                     /locus_tag="VFT03_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU4847658.1"
                     /translation="MSEKKTSMIARYAGGERGARAAARAGSAVVVVDAFRASTTIAVL
                     VTKGARVVPVASIEEARSYVGADLRAGERGSAMVAGFDFGNSPTEILASDVPVGSTVV
                     LSTTNGTRVVEAARGAPAILTGAFVNASALADELAARGYNSLVVVGCGWEGRRASEDE
                     AAAGAILQRLACRGAELDERARRVVEAYSSRPAGRLRNNSAARRLKRLGYERDLDFCL
                     AEDAVPVVPRLVNGAFVGGR"
     gene            7653..7865
                     /locus_tag="VFT03_05630"
     CDS             7653..7865
                     /locus_tag="VFT03_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847659.1"
                     /translation="MIRQVTLTISHEKTREITREEVDRAIDVIERHLEKEARTRNLLD
                     EQAIMRPKDLCKQQGLDMVAELRGMM"
BASE COUNT         1429 a         2632 c         2620 g         1688 t
ORIGIN      
        1 agctcatcga gcatcgtcga tgcttcatct acggtaagcg aggtccacat ggcggccgta
       61 gcgggggtga tctcgcgccg ttcggcgagc gtgtgcaaaa gctcgccttc tttgaccttt
      121 ctttcatccg aagtctcgag gccgggtctg tccagcactg agagcgcgat cggcacgaca
      181 gaggcgaaga ccatggcgag ccaccagaag ctcgttaggg gagccagaac gacgcatatc
      241 cagcctgagg cgatcaggta caacgcggac tgccagttga cccacgatgc aacttcccgt
      301 ttcgctgtcg agcctcgcgc tctgagatcc ccgctcctgc tcggagcgcg gccttcacca
      361 tcgctcgtgc ggtgttccgg ttccacgatc tcacatacgt gtccgggcgc cgcttgtttc
      421 acgaccgaat gcattcgtcc actagtggag acccggggga ggccggacta cggaccttcc
      481 gaccggcatc tccagaggtg gtgcgaacct actggcccca ggtctcgcgc agcacgcgga
      541 cccagttctc gtgcgccagc atcttcagcg ccgcgtcgtc gtagccgcgt tcgcgcagct
      601 ccgcgagcag attcgggagc cctgaggcgt cgcggatccc gagcgggacc ttcgccccgt
      661 caaagtccga gccgaagccg acgcgctcga cgccgaccct ctcaaccagg tagtccacgt
      721 ggcgcaccac gtcctcgatg ggcgtgtctt cctcctccgc gccgtcctcg cgcaggaagg
      781 cgacggcgaa gttcacgccc accatcccgt cagagtcgcg tatcgcgtcc agctgtctat
      841 cggtgaggtt gcgcgaggtc ggacacaggg cgtgagcgtt cgagtgggtc gcgacgagcg
      901 gtgtctccgt tatacttgct acgtcccaga agccgcgctc gttcaggtgc gagaggtcga
      961 gcaagacgcc aagccggttg cacacgcgga cgagctcctt ccccgcatct gtcagccctg
     1021 ggcccgtgtc cggaggagac gggaaccgga aggggacgcc gtgagcgtag gcgttgggcc
     1081 ggctccacac aaggccgagc gagcggaggc ccacctcgta gaggtcttcc agagcgccgg
     1141 ggtccgggcc gaggttctcg gcgccttcga agtggaggat cgccgcgagc acgccttcgc
     1201 tcaggcaaga ctcgatctct cgagcagtcc gaactacttc gagcttgtct tcagactcgg
     1261 cctccaggtc gaacagaacc ccaaccagtt cgtctgcctt tctcctggca tggaccgggt
     1321 caagcggcgg cgcatccgcg acttcgtagc cggtcttggt cagggtgagc gcagactcct
     1381 cggtccagcc atcattctcc gggttccgaa cgtagcaggc gaagaagccg ccgccgaagc
     1441 ccccctccct ggcgcgcggc aggtcgatgt gcccgacgtc gctccgctcg aagaagttcc
     1501 gctcctctcc gcgctccggg aagcggagct tgagcagcgt gtcattgtgg ccgtccagta
     1561 caggtatatt cgtcatccgg tcgatgatac aggcccatcg aaggcaggaa aaaggggccg
     1621 cagagcggcc cctctatcta tcgcggcgat gacccgaagc ctcctctaat acttcgggac
     1681 gctcgagtct atctcctcgc tccaggcgtc gataccgccc ctgacgttgt aaacgttctg
     1741 gaagccggcc tccttgagga gcttcacggc cctcgcgctg cggccgccgc tcttgcagtg
     1801 gacggccagc gtctcgtccc ggtcgagctc gaccaggcgc tcaggcagct cggcgagggg
     1861 gatgagcctc gcgccgatgt tcgctacctc gtactcgtgc ggctcgcgca cgtcgaggac
     1921 ggagaccttc gagccgttct gcaggcgtcc ctcgagctcc tggaccgtga tctccggcac
     1981 ttcgtcggcc tgcgcctggg cgttggcctg ggggatgccg cagaactcct ggtagtcgat
     2041 gagctccgtg acggtcgggt tctcgccgca gaccgggcag ttcgggtcct tgcgcagctt
     2101 catctcgcgg aagcgcatgc ccagggcgtc gtagagcagg aggcggccta tcaggggctc
     2161 gccgatgccg agtatgagct tgacggtctc cgtggcctgg atggtgccga tcgcgcccgg
     2221 cagtatcccg aggaccccgc cctcggcgca gctcgggacc aggccgggag gcggcggctc
     2281 ggggtacagg cagcggtagc acgggccctc ttcggcgtag aagacgctcg cctggccctc
     2341 gaagcggaag atagaaccgt acacgttagg cttgccggtt aggacgctcg cgtcgttcac
     2401 taggtagcgg gtcgggaagt tgtccgtgcc gtccacgatc acgtcgaagt cctcgaagat
     2461 ctccagcgcg ttctcgctgc taaggggctc tccaaaggtc tcgacgttca cgttcgggtt
     2521 tatgtcctct atcgtctcgc gggcgctctc cagcttggga cgccccaggt cggaggtgcc
     2581 gtggatgatc tgccgctgca ggtttgactc gtccacgacg tcgaagtcca cgacgcctat
     2641 cgtcccgatc ccggccgccg cgaggtacat cgcgagcggg ctccccagcc ccccggcccc
     2701 gatggtcaag acccttgcct gcttgagctg cttctgccct tcgagggcaa cctcgggcat
     2761 gatcaggtgc cgactgtagc gcgcgatctc ttcattcgag agatcgaccg acccgttggg
     2821 cgactctacc aatggctttc ttgtctgcat tttgtgggaa ggctccttac gaaatgaatg
     2881 tataggtgcg aaacctgcgt ctataaacta cctacatcgc cttccggcac agcctccggc
     2941 gatggagggg atgatcgaga gctcgtcgcc ctcggagacc tctgtgtcgg cgccgtcgag
     3001 gtagcggatg tcctcgtcgc ccttgtatac gttgacgaag gagcggagct tgccgtcgcc
     3061 ggtgtagagg tgctgcctga ggtctgggta ctcggaggcc aggtttccga gcgtctcgcc
     3121 cgcggtctcg ccgccaacct ccacctcgga gtctcctccg gtgtactgtc tcagcggcgt
     3181 tgggatcttg atcttcggca tcaaaggctc ctttgcaatt ctaacttact atcggctctt
     3241 cttcgtaccc cgagcggtcg tctcttaata cccaggagcg caggtcctcg accccgttct
     3301 cacctacgga ggcgatgacg taggagtaga agggccacgc gtgctccctg tcgtatcgag
     3361 agggctcggc agggtggtcg gggtgggagt ggtagaagcc gagcacgtcc atgtccctct
     3421 cggcggcccg ctcctcgaac tccttgatct tgagcggctc gatcagaaac ctgcggctgc
     3481 gctcgtcctc gtgagtgttc tctgcgcgcc agacgtcgac cacgatcttc atcccatcga
     3541 ggttcatccc gaccatggcg cctgcgcact cctccgggta tgactctctg gcgtgctcgt
     3601 ggatgtggtc aacatctccc cgtcctatct tgagcggcac gtcgtcctaa tcctcccaga
     3661 acctctcgga gaggtacttg tccgccccgt cgcacaggat ggtgacgacg acaccgttct
     3721 cgatctcgcg cgccacctcg agcgccgtcg cgaccgcggc cccggctgat atgccgatca
     3781 ggaccccctc ctcgcgcgcc acgcgcttga ccatctcgta ggcgtcctcc gtcgaggccc
     3841 gcctgttctc gtcggctacg ctcgggtcgt aaatgggtgg cacgatcgcg ctctccatgt
     3901 gcttcatgcc ctcgagcccg tggaacggcg actccggctc gaacgagatg acctttatgt
     3961 ccgggttgta ctccttgagg cgcctcgcgg tccccacgaa ggtcccgctc gtacccaacc
     4021 ccgcgacgaa gtgcgtgatc tcaccgcccg tctgctccca gatctccggc gccgtggact
     4081 cgtagtgcga cctcggattg gccgggttgc tgtactgatc agggtagaag tacttctccg
     4141 gctcctcggc gtagagccgc ctcgcctctc tgatcgctcc gtcggagccc tcgccagggt
     4201 ccgtcaagat gaattccacc ccgtaggcgc gcaggatctt ctttcgctcc tggctggcgt
     4261 tactgggcat gcacagcctg actttgtagc cgagggcggc acagatccag gcgtacgcta
     4321 ttcccgtgtt cccgctcgtc gcgtcaagga tggtcttctc tggcgtgaga gcgccgctct
     4381 tctcgccgtc tcggatcatc cacagcgcgg ggcggtcctt gaccgaacca ccagggttgt
     4441 accactcggc cttgccaagg atcctgaccc cgggcacgtc agcagagatg ctggggaact
     4501 ctagcagcgg cgtgttcccg accagctccg ttactttagt gcctgttggc tcattaatcc
     4561 gagtaatcat gtcgggatta ttatacaagg ctggcctatt gcgtccagcg aacctcgaga
     4621 gagatacccg accaacgtga tagccggaag gctccctctc cggccctccg gctatggttg
     4681 gtgcgctgtg aaagattata ctcgagtcgc gccaccttcc gctacggcga aaatcgccga
     4741 ttatgccaaa gattcaagaa tttcggggag cgttctgcga cggcaggtaa agatgggagt
     4801 gtcgagctcg gtgtacgagg aggcttcgct ctccctgagc tccatcatca ggttctggaa
     4861 cttctccggc gactcggcct cgaacgagag gatgaactcg tagtcgtcta tcccgaagca
     4921 gtaagcggtg ttgttcttca cggggtaatg tttgcggcct atcttcatgt gctcgtccat
     4981 catacgctgg cgctcttcga gggggagttg gtaccaggca cgggtcttga cgaaagggta
     5041 gacgaaaagg tagtcgccct caccggggac gatgtagcgt ctgttctcga agccgggcgt
     5101 gtgctcgccg acgtagatcg aacgcctgct gagggcgaag taggagtacg tcaccgtcag
     5161 atacctgccc agtcccgtct tcatgagcgc gctcgtcatc ttctcgaagg cgtctagatc
     5221 gtagcctatg cgccagagca tgaagtcagc gtcgcttctc agacccatga gcgagaacga
     5281 gcgcaggagc atgtcctggc tgtgttcctc gatcgcttcc aggaactcct ttttaccccg
     5341 ctctcgctcc tcgtccccca tacgccgcca gagaggatcc agcttgaaga agacatagtt
     5401 gacgtactgc gccgggagac tctcctcctg ggcagcttgc tgctgtgtcc tggtctcgct
     5461 cactggtcct cccttcggtc ggtcataggt ttcagacttc aggcttttgc cggcgagagt
     5521 ctcgacgcgt ggtctatatt cgtgtgcgca aaaggtgcgt tcctcctcgc cctctcccct
     5581 gatgcctaat gcctgatgcc tgatgccttc tcgtctgaag cctccagccc gaagttgttc
     5641 tcgtaaagct cggcgacgcg ctcgagctca tcctgtgaga gatcaggggt atcgggggct
     5701 gctgcgaact cgtcgagttg ctcatcgtcg tagatgtttg gtaacgtcga gacgatctgt
     5761 ggtgagacga ggatgaactt cagggcagcc tgacctagag tacgttcgcc ggactcggtt
     5821 aggaaagaga gttgctcgac cttcttcaag ccgtcgagga gccactcctt cgagcggtgg
     5881 cggcggtggt cgttcttcgc gaaggtcgta tcttcggtgt acttcccctc gagcatgcca
     5941 gaggagtggg ggacgcggac gatcaggctc gtgtccgtct caagggcggt ttttatcaag
     6001 gattgaccgg ggtcctgttc gaggaggttg tatatcatct gcacgcccgc gaggtcgcgc
     6061 tcgcgcatag ccttctcacc ctcctcgagc cagccgatct ttggcccaag ggcgacgccg
     6121 taggagcgga tcttgccttc ctctacgaac tcgttcatga gctcgaagag ccggtcgttc
     6181 tctatggcgt ccatcttggt gttgtggagc tggaggaagt ctatgtagtc ggtgccgagc
     6241 cgtttgagac tctgctccag ggcgaaacgg atgaaatcct ccgaccagtc ctgtggacgc
     6301 tcctgctggc cacggcgggc ggtatgattg tagaagtcgt agccgatctt ggtggagatc
     6361 acgacctcgt cccgcatccc gccgaaggcg tcggccagaa gcgtctcgcc cttgcccgag
     6421 ccgtaggtgt cggcggtgtc gaagtagttg atccccttct cgaaggcgcg cctgagcagg
     6481 cgcaccgacc gctcgtcgtc cacctccccc caccacccgg tagagaccgt ccacacgccg
     6541 aaaccgacct cggagatccg tatatccgtg ccggcaaggt tgcgatatcg catcctggat
     6601 ccctcgtctc ctagtaagaa ctcacgacaa agagttacta cattctatcg ccgttcgctg
     6661 tggagatgtt ctcaagcggg ttttccgtat tgagcgagag agaagccgga gcttgcgccc
     6721 cggcttctct ctgttgccgt cgcgagccgg ctgccagctc cacggatgcc gcttcgtcca
     6781 tataatgcgc cgggtgtcgg agaagaagac gagcatgatc gcccgctacg ccggcggcga
     6841 gcgcggggcg cgggctgcgg cgcgcgccgg ctcggcggtg gtcgtggttg acgccttcag
     6901 ggcgagcacg acgatagcgg tcctcgtaac gaagggcgcc cgcgtggtgc ctgtcgcctc
     6961 gatcgaagag gcccgctcgt atgtgggggc ggacctccgc gcgggggagc gtgggagcgc
     7021 gatggttgcc ggcttcgact tcggcaactc tccgacggaa atcctggcgt ccgacgtgcc
     7081 cgtcggctcc accgtcgtcc tctcgaccac gaacggcacc cgtgtcgtcg aggcggccag
     7141 gggagcgccc gccatcctga caggcgcctt cgtcaacgca tccgctctgg ccgacgaact
     7201 ggccgccaga ggctacaact ccctggtggt ggtcggttgc ggctgggagg gccggcgcgc
     7261 ctccgaggac gaggctgcgg cgggggcgat cctgcaacga ctggcctgca ggggagccga
     7321 gctggacgag cgcgcccggc gcgtcgtcga ggcttatagc tcgcgtccgg ccgggcgcct
     7381 gcggaataac agcgcggcga gaagactcaa gcgcctcggg tacgaacggg acctggactt
     7441 ctgcctcgcg gaagacgccg tgcccgtcgt gccgcgcctc gtgaacgggg cgtttgtggg
     7501 gggtagatag ggaggctcga aaacttcggc cccggggcca atgcggaaag cgaaaattcg
     7561 tcttgacaag ggattggcgc cccagtactc tggggttagc cagggcggac caaacgttac
     7621 aaaggttcgc tcgactccac gatgggaagg aggtgatccg acaagtgact ctcaccatct
     7681 cgcacgaaaa gacgcgcgaa atcacccgcg aggaggtgga tcgggccata gacgtcatcg
     7741 agcgtcacct cgagaaggag gcccgcaccc gcaacctgct ggacgagcag gcgatcatgc
     7801 gcccgaagga cctgtgcaag cagcagggcc tcgatatggt cgccgagctg cgcggcatga
     7861 tgtaacccct agtggagccg ccgcgctagg cgaagccaag agcgcggtcg agggcctacg
     7921 aagggggatg cacagtgaaa gcgtcccggg tctccacggg aggcccagcg ggctgtccat
     7981 cccccttcgc ataggtcaat gaagagggtc gggaaaaacc cggccctctt tctctgtgtt
     8041 cgtttccggg cggtatccag gaccttcttc aacaggtgct accgggcgta cttcttgatc
     8101 tcgcagcgcg taagatggtc gaagggatct gcgagaacct tcctgccctg ccagccgtgt
     8161 ctgggaacga gtcggttctc atcggtttta cctctcctga tcgtagcgag agggcaggat
     8221 ggccctcccc aatgaacgtt acaatagtat cattactctc aagaaggata gcgtgaagcg
     8281 atcagacagc cgggttgttt aggagcgaaa tgctcggttc gtgaggaggg gacgctttga
     8341 gacaaggaga agatcttgag cgcgaaggt
//
LOCUS       DASTUV010000281         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_825427, whole genome shotgun sequence.
ACCESSION   DASTUV010000281 DASTUV010000000
VERSION     DASTUV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825427"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VFT03_05635"
     CDS             complement(<1..166)
                     /locus_tag="VFT03_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEU4847660.1"
                     /translation="MCGRYTLASPTERLAEEFGVDASSVELAPNYNVAPTQGVAAVLE
                     EGGQRRLEVLR"
     gene            complement(244..723)
                     /locus_tag="VFT03_05640"
     CDS             complement(244..723)
                     /locus_tag="VFT03_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4847661.1"
                     /translation="MADRRRRVELVIFVGLQASGKSTFFRERFAATHEHVSKDLFRNN
                     RDRNRRQAQLIEAALGKGVSVVVDNTNPTVEDRRSLIRLGRRFGAEVVGYYFASTVRQ
                     SIGRNRQRAGADRVPDVAVYATAKKLERPAYREGFDELFSVRIYGDSAFDVRPEPRG"
     gene            900..1847
                     /locus_tag="VFT03_05645"
     CDS             900..1847
                     /locus_tag="VFT03_05645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU4847662.1"
                     /translation="MAKIGYFLSCEEWGPLELIEQARMAEEAGFEALWISDHYHPWND
                     EQGNSPLVWSVIGGLSQSTSLPVTTGVTCPTMRIHPAIIAQAAATSKVMLEGRFSLGV
                     GTGENLNEHILGQRWPRTSVRLEMLEEAIEVMRLLWEGGTRSHYGKHYTVEKARVYTM
                     PDEPPDVLISGFGPKSTRLAGRVGDGYCHTAPDAEMLSLFRESGGGNKPAHAGTKVCW
                     SEDVATARKTAHRLWPNELLPGELAQELATPRHFEQASSLVTEEMMAVPCGPDPEEHL
                     ESIRQYVEAGFDEIYVQQIGPEQEGFFRFYEREILPKLR"
     gene            1905..2108
                     /locus_tag="VFT03_05650"
     CDS             1905..2108
                     /locus_tag="VFT03_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEU4847663.1"
                     /translation="MPYDNSSDLPDSVRNNLPKHAQEIYRAAYNSAEDQYGEEERAHR
                     VAWSAVENKYEKNDQGNWVQKND"
     gene            complement(2190..>2778)
                     /locus_tag="VFT03_05655"
     CDS             complement(2190..>2778)
                     /locus_tag="VFT03_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU4847664.1"
                     /translation="DTTRAGALTVDVAILGELDAPPVLRSGARAGDLLAVTGELGASA
                     AGLLALESGASGPKRLVEKHLRPTPRVEAGRAAARIGVGAMIDLSDGLASDASRVCER
                     SGVGCRVDLDLLPVAEDTREFVESLGQDPEVLAATGGEDYELLISASEPAHEALAESV
                     EVPVTRIGEVTDGDGVVFWRGGEVVEGLSGWDHFG"
BASE COUNT          523 a          951 c          858 g          446 t
ORIGIN      
        1 atctgagcac ctccagccgc ctctgcccgc cttcctccag taccgccgcg actccctgcg
       61 taggagcgac gttgtagttc ggcgcaagct cgaccgaaga ggcgtctacg ccgaactcct
      121 cagcaagtct ctccgtcggg gacgcgagtg tgtatctacc gcacataatt caccttccga
      181 acgccagatc ctccaggcta attgtacccg gcttccggct ccctcccatc ccccaactgg
      241 cgctcaaccg cgaggctccg gccggacatc gaaggcggag tcgccgtaaa tgcgcacgct
      301 gaagagctcg tcgaacccct cccggtaagc aggacgctcg agcttcttgg ccgtcgcgta
      361 tacggcgacg tcgggcaccc ggtccgcacc ggcgcgctgc cggttgcgcc cgatcgattg
      421 gcgcaccgtc gaggcgaaat agtagccgac gacctccgcc ccgaaacggc ggccgagacg
      481 gatcagggac cgccgatcct ctaccgtggg gttcgtgttg tccacgacta ccgagacgcc
      541 tttcccgaga gcagcttcga tcagttgcgc ctgcctcctg ttccggtctc tgttgttgcg
      601 gaacagatcc ttgctgacgt gctcgtgggt cgcggcgaac cgctcgcgga agaacgtgct
      661 cttccccgaa gcctgcaggc caacgaagat caccagttcc acacgcctcc tgcgatcggc
      721 cattacgagg tgccataatg gtactcgtcg accgcatgga accatagtcc ttcgtgtggg
      781 tggcgtcgag cctcgaaacg tagcgcgcat cgaagacgga gtaggtatct tcggcggggg
      841 gtataccagg acggcgacgc cacagtgatg aactcgaaac ccgcgaagga ggttcgcgcg
      901 tggctaagat aggctacttt ctgtcctgcg aggagtgggg gccgctcgag ttgatcgagc
      961 aggccaggat ggccgaggag gccggcttcg aggcgttgtg gatctcggac cactaccacc
     1021 cctggaacga cgagcagggc aacagcccgc tggtgtggag cgtgatcggg ggcctgtccc
     1081 agtccacctc gctcccggtc acgacaggcg tgacctgtcc cacgatgcgt atccacccgg
     1141 cgatcatcgc ccaggccgcg gcgacctcga aggtgatgct cgaaggacgc ttctctctgg
     1201 gcgtcgggac gggcgagaac ctgaacgagc acattctcgg gcagcgctgg cctaggacga
     1261 gcgtaaggct ggagatgctc gaggaggcca tcgaggtcat gcgcctcctg tgggagggag
     1321 gcaccaggag ccactacggc aagcactaca ccgtggagaa ggcgcgcgtc tataccatgc
     1381 ccgacgagcc gccggacgtt ttgatctccg gtttcggccc gaagtcgacg cggctcgcgg
     1441 gtcgcgtcgg cgacggctac tgccataccg cgccagacgc cgagatgctt tcgctcttca
     1501 gggagtcagg cggcgggaac aagcccgccc acgcgggcac gaaggtctgc tggagcgagg
     1561 acgtggcgac ggctcgcaag accgcgcacc ggctctggcc caacgagctg ctccccggcg
     1621 aactcgccca ggagcttgct accccgagac acttcgagca ggcgtcctcg cttgtcaccg
     1681 aggagatgat ggccgtcccc tgcgggcccg atcccgagga gcacctcgag tccatccgcc
     1741 agtacgtcga agcgggcttc gatgagatct acgtgcagca gataggcccc gaacaggagg
     1801 gtttcttcag gttctacgag agagagatcc ttccgaagct ccgataagcg aagcatcgtc
     1861 tcttcgggta taaggtccgg gcaatacaac gaaagagagg taccgtgccc tacgataact
     1921 cgagcgatct gccggacagc gtcaggaaca acctccccaa gcacgcccag gagatctacc
     1981 gggcggccta caactccgcc gaggaccagt acggcgagga ggaacgcgcc catcgcgtcg
     2041 cctggagcgc cgtcgagaac aagtacgaga agaacgacca gggcaactgg gtccagaaga
     2101 acgactgagc ttcgccaggc tacggccctt ctcgaatcga cgagccccgc tgccacccac
     2161 tattccggga gagcaataaa ccccgatacc taaccaaaat gatcccagcc cgagagaccc
     2221 tcgacgacct ctcctccgcg ccagaagacg acgccatctc cgtccgtgac ctcgccgatc
     2281 ctggtgacgg gcacctcgac gctttcggcc agcgcttcgt gcgctggctc ggaggcagag
     2341 atcaggagct cgtagtcttc cccaccggta gccgccagca cctccgggtc ttgtccgagg
     2401 gactccacga actcccgcgt gtcttcagcc actggtagaa gatccaggtc cacgcggcac
     2461 cccaccccgc tccgctcgca gacgcggctc gcgtccgagg cgaggccatc cgagaggtct
     2521 atcatcgccc caactccgat ccgggccgcc gcgcggcccg cctctactcg aggggtcggc
     2581 cgcaggtgct tttcgacgag acgtttcggg ccggaggccc cgctttccag ggccagcagg
     2641 cccgccgccg aagcgccgag ctctcctgtc accgccagca gatcccccgc tctcgcgccc
     2701 gagcgcagga ccggcggcgc gtcgagctcc ccgagtatcg ccacgtccac cgtaagggcg
     2761 ccggcgcggg tcgtgtcg
//
LOCUS       DASTUV010000282         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_149112, whole genome shotgun sequence.
ACCESSION   DASTUV010000282 DASTUV010000000
VERSION     DASTUV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149112"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFT03_05660"
     CDS             complement(<1..1166)
                     /locus_tag="VFT03_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847665.1"
                     /translation="MSNGGTATHAVESRIPARMDRLPWARWHWLVVFALGITWILDGL
                     EVTIVGNIAGRLVEPASGLNLTEGQIGLAAGIYIAGACTGSLFFSYLTDKYGRKRLFL
                     ITLGVYLVFSFLTGFSWNFWSFVFFRFMAGTGIGGEYSAIYSAVDELIPARFRGQVAL
                     AISGSYWIGTILGSGAAILLLNENIINQYYGWRLAFWLGALLGICVLLIRRYVPESPR
                     WLLTHGRTEEAEETTDAIEEEVRSETGRDLPPAEGPPLVVEQRESIGFSVVFKAMFKM
                     YPKRSVLGLTLMGSQAFLYNAIFFTYALVLTKFYNIDPSHVPYYIFPFAIGNVLGPWL
                     LGRLFDTVGRVPMIAGCYFISGLLLAGTGYLFYLDVLNAVTQTALWCVIFFFASA"
     gene            1388..1867
                     /locus_tag="VFT03_05665"
     CDS             1388..1867
                     /locus_tag="VFT03_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4847666.1"
                     /translation="MSEFPAKILVATDGSEDAMLAARAAISLAHDTGAELHVVHVGPA
                     HVYPPRAAGPALPTGTTEELRKEAQAVLDWQVDEITKAGGEIAEAHLRMGHPADEILH
                     LSEQIVPGLIVVGNQGLGSRFSRLRRFLMGNVSEKVSRYARCSVMVVRKDMYEQPQV"
     gene            complement(1887..>2577)
                     /locus_tag="VFT03_05670"
     CDS             complement(1887..>2577)
                     /locus_tag="VFT03_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4847667.1"
                     /translation="LLEDLGPDLILTQGLCEVCAVSTSLVERAVAGMRKEPEILTLNP
                     TSLRAVLDDSVRIGEALGRGDETRQKVAALEGRLASVREAVAELSRPRVSCIEWLDPP
                     FSAGHWVPEMVDIAGGEEVLAGPGERSARLSWDEVFEAAPEVIVLMPCGFDTDRAVEE
                     AGRVLPGLPGWGDLPAVREGQVWAVDANSHFSRPAPRLVEGVEILARILHPEASLGEP
                     EIALPFSRVAG"
BASE COUNT          486 a          849 c          822 g          420 t
ORIGIN      
        1 gcggaggcga agaagaaaat gacgcaccac agggccgtct gcgtgacggc gttcaggacg
       61 tcgaggtaga acaggtagcc cgtcccagcc agcagcaggc ccgagataaa gtagcaaccc
      121 gctatcatcg gcacccggcc cacggtgtcg aagaggcgcc cgaggagcca cgggcccagc
      181 acgttcccga tagcgaacgg gaatatgtag taagggacat ggctcgggtc tatgttgtag
      241 aacttcgtca gaaccagcgc gtaggtaaag aagatcgcgt tgtagaggaa ggcctggcta
      301 cccatgagcg tcagcccgag gacgctccgc ttcgggtaca tcttgaacat ggccttgaag
      361 accacgctga accctatgct ctcccgctgt tctactacga gcggcgggcc ttcggccgga
      421 gggaggtcgc gtccggtctc acttcgcacc tcctcctcta tggcatcggt ggtctcctcc
      481 gcctcttccg tgcggccgtg ggtgaggagc cagcgcgggc tctcagggac gtagcgccgg
      541 atcaggagca cgcaaatgcc gaggagtgcc cccagccaga atgccagcct ccagccgtag
      601 tactggttga tgatgttctc gttgagcagc agaatcgcgg ccccgctacc aaggatcgtc
      661 ccgatccagt agctgccgct gatggcgagc gcgacctgcc ccctgaagcg cgccgggata
      721 agctcgtcca ccgcggagta gatcgccgag tactccccgc cgatgcccgt gccggccatg
      781 aagcggaaga aaacgaagct ccagaagttc cacgagaaac cggtgaggaa gctgaagacc
      841 aggtacacac caagcgtgat gaggaagagc ctcttgcgcc cgtacttgtc ggtcaggtag
      901 gagaagaaga gcgaccccgt gcatgccccc gcgatgtaga tgccggcggc gagcccgatc
      961 tggccctccg tgaggttgag acccgaggcc ggctccacga gcctgccggc tatgttgccg
     1021 acgatcgtca cctcgagccc gtcgaggatc caggtgatgc ccagagcgaa gaccaccagc
     1081 cagtgccacc tggcccacgg caaccggtcc atcctcgcag ggatgcgact ctcgaccgcg
     1141 tgcgtcgctg ttcctccgtt gctcacccca cgactcctct ctgctcggtc gctacggtcc
     1201 accgcggatg cgtgatatgc cgcatctacc ctctgcgtgc gatctttaat ccccgtttca
     1261 cattcactcg ttcgtatctc tgtttgccaa tcgtcgccgg gaggtatcaa tggtggttac
     1321 ccgctggggc gtcccgacga cgccttctga ggaccggcac ggtgaggtta ttgacaagga
     1381 ggctgagatg agcgagttcc cggcgaagat cctggtcgcg accgacggct ccgaggacgc
     1441 gatgctggcc gccagggccg ccatctcgct cgcgcacgac accggcgcgg agttgcatgt
     1501 cgtccacgtc ggcccggcac acgtctatcc ccctcgcgcc gccggccccg ccttgcccac
     1561 ggggaccaca gaggagctca gaaaagaagc ccaggcggtg ctcgactggc aggtggacga
     1621 gataacgaag gcgggaggag agatcgcgga ggctcacctg cgaatgggcc atcccgccga
     1681 cgagatcctg caccttagcg aacagatagt ccccggtctc atagtcgtcg ggaaccaggg
     1741 gctgggcagt aggttcagca ggttgaggcg ttttctaatg gggaacgtct ccgagaaggt
     1801 ctcgcggtac gcgcgctgct cggtgatggt ggtgcgcaaa gacatgtacg aacaacccca
     1861 ggtgtaggaa cccggccggc tccagatcac ccggcgacgc gcgagaaggg cagcgctatc
     1921 tccggctccc caagggatgc ctctggatgc agtatgcgcg ccaggatctc gaccccctcg
     1981 acgagccgcg gcgcagggcg gctgaagtga gagttcgcgt ccacggccca cacctgcccc
     2041 tccctaacgg ccggcaaatc accccagcct ggtagccccg gcagcacgcg gccggcctct
     2101 tcgacggccc ggtcggtgtc gaagccgcag ggcatcagga cgatcacctc cggggcggcc
     2161 tcgaagacct cgtcccacga gaggcgcgcg gagcgctctc ccggcccggc gaggacctcc
     2221 tctccgccag cgatatctac catctcgggt acccagtgcc cggctgagaa gggaggatcg
     2281 agccactcta tacaactgac gcggggacgc gagagctcgg cgacggcttc tcttacgctg
     2341 gcgagcctcc cctcgagcgc ggcgaccttc tgcctggtct cgtcgccacg acccagggcc
     2401 tcgcctatcc tgacggagtc gtccagtacg gcgcgcaacg aggtcgggtt gagggtgagg
     2461 atctcgggtt ccttcctcat cccggctacc gccctctcga cgaggctcgt ggagacggcg
     2521 cagacctcgc agaggccctg ggtgaggatc aggtccggcc cgaggtcctc gagcagc
//
LOCUS       DASTUV010000283         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_351172, whole genome shotgun sequence.
ACCESSION   DASTUV010000283 DASTUV010000000
VERSION     DASTUV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351172"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFT03_05675"
     CDS             <1..457
                     /locus_tag="VFT03_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847668.1"
                     /translation="ITVSEGSSGRLGAVRTAARHLKRGGALLTFPGGEIEPDPAVLPG
                     AQEALGRWSESLDLFARLVPGLTVVPAVVSGVLSPTALRNPLIFVRRQPRDREWLAAT
                     LQMLTPALRDVTTRVAFGEPVCAEEVTDGNVRQAVLGETRRLIERCSAR"
     gene            442..1713
                     /locus_tag="VFT03_05680"
     CDS             442..1713
                     /locus_tag="VFT03_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847669.1"
                     /translation="MLGKVASGRRRGWSPLAFIFATVFVDMLGYGLVIPLLPFYAREY
                     AAGAALVGLLGSLYAAMQFVGGPFLGGLSDRTGRRPVLVVCLLGASLAYLLLGLAETI
                     FLLFAAVVLAGAAGGTQATAQAYIADSTSPERRARGLGLIGAAFGLGLMAGPALGGLL
                     SLYSLHAPAFTAAALALANATFGFFVLPESLPAKLRTPTPLRRLNPLSQLRGILGIRS
                     IRWYLLAVFLLNLSFAGLLTNFPLFSNARFGWDAPSNAFFFAFVGACAVLTQGVLIGQ
                     LQPRFGEARLLIGGLSVMSAGLGLVAIVPYGPLLYPVVGVLAVGVGLAIPSLTALISR
                     QVSGREQGRVMGGLQAVISVTLIVGPVVAGLAFDHLGVPTPYWIGALLAALALLAASA
                     AVKPGHNSAPTKQSFAKRPAVEADALRRHGE"
     gene            1703..2023
                     /locus_tag="VFT03_05685"
     CDS             1703..2023
                     /locus_tag="VFT03_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847670.1"
                     /translation="MENNVLRIGIVVLTLATALIHLQLNFPDPVFILNGLGYLTLLAA
                     LFLPIPRIARYRNVVRFVLAGYAALTIFLWILIGARTPIGYIDKVIEIVLISLLLLDA
                     RRSN"
     gene            complement(2068..>3064)
                     /locus_tag="VFT03_05690"
     CDS             complement(2068..>3064)
                     /locus_tag="VFT03_05690"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEU4847671.1"
                     /translation="WFSEIATKGYTDGAPASTAFFPLYPMLVRLGTVFGGGPALWGVL
                     ISLTATLFALFFLYRIAETLHGTRSARAATLALAFFPTAFFLNAAYSEALFLALSTGA
                     VWAAIVRRNLLLAGLFGALAAATRNLGVVLILPLFFEWLRYRREFGVRGLAGIALVPA
                     GFLGYAGFLWARFGDPLVSARQQDEHWGRELANPFATLGDAWQAAGDGMNYLHHPQIL
                     FFSSSATPTLEASNTLNLAFLALFLILLVVGSFVLPPGLSLYAFVVVLLPVLTPAPSF
                     PLMSLPRFMLGAFPIFLVLGYLLSRCRPALVLWLLLSAGTGVALTALFTTWRWVA"
BASE COUNT          483 a         1036 c         1043 g          502 t
ORIGIN      
        1 gatcaccgtg tccgaggggt cctcggggcg gctcggggcg gtgcggaccg ccgcgcggca
       61 cctgaagcgt ggcggggctc tgctcacctt ccccggcggg gagatcgagc ccgaccccgc
      121 ggtgctgccc ggggcgcaag aggcgctcgg gcgctggtcc gagagcctcg acctcttcgc
      181 gcggctggta cctggcctca cggtggtccc ggccgtcgtc agcggggtcc tctcccccac
      241 cgctctccgc aaccccctga tcttcgtccg tcgccagccg cgggaccgcg aatggctcgc
      301 ggcgacgctg cagatgctga cgcccgcgct gcgagacgta accacgcggg tagccttcgg
      361 agagcccgtg tgcgccgaag aagtcaccga cgggaacgtc aggcaggcgg tgctgggaga
      421 gacgcggcgc ctgatcgagc gatgctcggc aaggtagcat ccggccgcag gcggggctgg
      481 tcgccgctgg cgttcatctt cgccacggtc ttcgtggaca tgctcgggta cgggctcgtg
      541 ataccgctcc tcccgttcta cgcccgggag tacgccgccg gggcggcgct cgtcggcctg
      601 ctcggctcgc tctacgccgc aatgcagttc gtcggcggac ccttcctcgg gggcctctcc
      661 gaccgcacgg ggcgccgccc ggtcctcgtg gtctgcctgc tcggcgcgtc cctggcctac
      721 ctcctgctcg gcctggccga gacgatcttc ttgctcttcg cggcggtcgt gcttgccgga
      781 gccgcgggcg gcacccaggc gaccgctcag gcctacatag cggacagcac ctctcccgag
      841 cgccgcgcga ggggactcgg cctgatcgga gccgccttcg gcctcgggct gatggccggc
      901 ccggccctgg gcggcctcct gagcctctac tcgctccacg ccccagcctt caccgccgcc
      961 gcgctggccc tggccaacgc gacgttcggc ttcttcgtcc tgccagagtc cctgcccgcc
     1021 aagctgcgca cgccgacgcc gctgcgccgc ctcaaccccc tgtcgcagtt gcgtgggatc
     1081 ctcggcatcc ggagcatccg gtggtacttg ctcgccgtct tcttgctgaa cctctcgttc
     1141 gcagggctgc tgaccaactt cccgctcttc tccaacgccc gcttcgggtg ggatgctcct
     1201 tccaacgctt tcttcttcgc gttcgtcggc gcctgcgccg tgctcacgca gggagtgctc
     1261 atcggacagc tgcagccacg cttcggagaa gcgaggctcc tcatcggcgg gctctccgtc
     1321 atgtcagccg gtctggggct ggtggcgatc gtcccatacg gcccgctcct ctaccctgta
     1381 gtgggtgtgc tggctgtcgg agtcgggctc gccatcccgt cgctcacggc gctcatctcg
     1441 cgccaggtgt cggggaggga gcagggcagg gtcatgggag ggttgcaggc cgtgatcagc
     1501 gtcaccctga tcgtcgggcc ggtagtcgcg ggcctcgcct tcgaccatct aggcgtaccg
     1561 acgccgtatt ggatcggtgc cttgctcgcg gcgctggcgt tgcttgcggc atcggcagcc
     1621 gtgaagccgg gacacaactc cgcgcccaca aagcagagct tcgcgaagcg gccagccgtc
     1681 gaagccgacg cacttcggag acatggagaa taacgtgctc cggatcggca tcgtcgtact
     1741 gaccctggca acggccctga tccacctcca gctcaacttc ccggatcccg tgttcatcct
     1801 caacggcctg gggtacctca cgctcctggc ggcgctgttc ctgccgatac cacgaattgc
     1861 acgttaccgg aacgtagtgc ggttcgttct cgccgggtac gcagcgctca cgatcttcct
     1921 ctggatcctc atcggagcgc gtaccccgat cgggtacata gacaaggtca tcgaaatcgt
     1981 cctgatctcg cttctcctgc tcgacgcccg ccgctcgaat tagcaggctt caggagattt
     2041 acctggtgcc tacggcctga agcctgctta cgctacccac ctccacgtcg tgaacagcgc
     2101 ggttagcgcc actcctgtgc cggcgcttag gagcagccag aggacgagcg cgggtctgca
     2161 gcgcgagagg aggtagccga ggactaggaa aatggggaat gccccgagca tgaagcgcgg
     2221 caggctcatg agcgggaagg agggcgcggg cgtcaggacc ggcagcaaaa ccacgacgaa
     2281 ggcgtagagc gagagccccg gcggcagcac gaaggaccca acgaccagca ggatcaggaa
     2341 tagcgccagg aacgcgaggt tgagggtgtt cgaggcttcg agcgtcggcg tggcggagga
     2401 gctgaagaag agtatctgag ggtggtgcag gtagttcatt ccgtcaccgg ccgcttgcca
     2461 ggcatctccc agggtcgcga aggggttcgc gagctcgcgc ccccagtgct cgtcctgctg
     2521 gcgggctgag acaagcgggt ccccgaagcg cgcccacagg aagccggcgt accccaggaa
     2581 gccggccggg accagggcta taccggcgag gccgcgcacg ccgaactccc tccggtagcg
     2641 gagccactcg aagaagagcg ggaggataag gaccaccccg aggttccggg tcgcagcagc
     2701 tagagcgccg aagaggcccg cgagcaacag gttgcggcgc acgatggcgg cccagaccgc
     2761 tcccgtggag agggcgagga agagcgcctc ggagtaggcg gcgttaagga agaaagccgt
     2821 cgggaagaag gcgagggcga gcgtggcggc cctggcggag cgtgtgccgt gcagcgtctc
     2881 ggcgatgcgg tagaggaaga acagcgcgaa gagagtggct gtgagggaga tcaagacccc
     2941 ccacaacgct ggccctccac cgaagaccgt ccctaatctg acgagcatcg gatacagtgg
     3001 gaagaaagcg gttgaggccg gagccccgtc tgtgtagccc tttgtggcta tctccgagaa
     3061 ccag
//
LOCUS       DASTUV010000284         6715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_115369, whole genome shotgun sequence.
ACCESSION   DASTUV010000284 DASTUV010000000
VERSION     DASTUV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115369"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFT03_05695"
     CDS             <1..243
                     /locus_tag="VFT03_05695"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4847672.1"
                     /translation="SVNEACAVGVAEGARVDPGPILAALEGMPNGFRSSMQKDVAAGH
                     PPEVDAIAGPILRGGREHGIDVSATRALVDRIVAPE"
     gene            complement(379..1083)
                     /locus_tag="VFT03_05700"
     CDS             complement(379..1083)
                     /locus_tag="VFT03_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU4847673.1"
                     /translation="MRPAEPRGEERLVRVAAGPVTLEGNLSLPERARGIVLFAHGSGS
                     SRHSPRNRYVARVLNEAKLATLLVDLLTPDEEAVDLRTAHLRFDIRLLAGRLVGVTDW
                     LTQYPDTRELRIGYFGASTGAAAALVAAARRPDAVGAIVSRGGRPDLAGPALARVRAP
                     TLLIVGGNDFEVVELNRRAFALLRCEKQLEIVPGATHLFEEPGALEEVARLACEWFER
                     YLTPTEAGSGIGKPAT"
     gene            1260..2294
                     /locus_tag="VFT03_05705"
     CDS             1260..2294
                     /locus_tag="VFT03_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU4847674.1"
                     /translation="MDGSKMLKEIREQPEVLGRVLDEGWGEVSAAARELRSRGFRSAM
                     LAARGTSDNAALYAKYLFEVILGIPTALASPSTFTLYETEMKLDDVLVIGISQSGEST
                     DVLETVRRSGELGASTLSVTNDEGSSMAAAAEFHFSLRAGEEKSVAATKSYTAELLIL
                     YLLVRALNGEEEPGAEVGRLPEAAREVLEASWKGTERYRYADHLVVTSRGYNFATAKE
                     AALKLMETTYVLAEAFSAADLRHGPIAMIGQDFPVIAIVPPGKVRQGMVSLVESLWER
                     GAEVAVISDDETLVDRAGVGFVVPASCPEELSSVLYAIPPQMLAHDLARLKRLDPDAP
                     RGLSKVTETW"
     gene            2374..3216
                     /locus_tag="VFT03_05710"
     CDS             2374..3216
                     /locus_tag="VFT03_05710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4847675.1"
                     /translation="MENESEKNGLPFELGVFDLDGTVLRRDLEISERTRAAMDELRRR
                     GVRLVVATGRRFEGAREHSGRLGFAGKDPLICFGGSMVRRMNGETLLHRTLPGDLGVE
                     VLEWAAGRNLHARVFLDERVITSPDTPAAFKHLRRYEQPGLSVVEEPADWLRNAGVEP
                     TKLVLVDYPDEVEGWLEEAQDAFRGRLFVTRSLPHYVEVGSIDGTKSRALRFLCERWG
                     VDPARALAFGDADNDIDMLRFAGHGVAVGGMTAGVRDAADAVAPPVHEDGVALYLEKL
                     LGDL"
     gene            3216..4037
                     /gene="mutM"
                     /locus_tag="VFT03_05715"
     CDS             3216..4037
                     /gene="mutM"
                     /locus_tag="VFT03_05715"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU4847676.1"
                     /translation="MPELPEVTVISEDIRSLAGGREVLRAGVFRPDVTNVEPEEFTRR
                     LVGRTLRGTGRRGKITLLDFGDVIGVIHLVISGRVLRLPAWTEPDRMNTAVLEFEGGL
                     VLAFTRLWLGYFHLYEPGTIDEHPLISRLGPDPFSEGFTPEYLAKAFERKASVKGLLL
                     DQSVVAGLGNIYVDEVLFAAGVHPTRKANTLGPEEILAIHAATRDILGRAIELRGTTF
                     DSYHDAFGESGEFQYQLEVFTRAGEPCPVCGTKVVKSRVAGRQTYTCPVCQHAPA"
     gene            complement(4061..4639)
                     /locus_tag="VFT03_05720"
     CDS             complement(4061..4639)
                     /locus_tag="VFT03_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847677.1"
                     /translation="MLIVVISFSALLLGGCSGVLGQTPREQANDAIVEANQSIAEHNK
                     LFEKARDTYAEVKQQIESGEDPSAERDRITEAKNTLEEARANLDDARESLATVQDLDV
                     DKDIKQYARLLSQAMDAQLAAEAKEVEFYDLLEEDPALENNRDKALDLLDEIGAGYEE
                     AEKAYGEAQDLADSKPKLIEAAPKSGNAPDNG"
     gene            4632..5075
                     /locus_tag="VFT03_05725"
     CDS             4632..5075
                     /locus_tag="VFT03_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847678.1"
                     /translation="MSTRGYIICAMDQQSLPFFTHLALRSFPGTLKRALGRFFKRRRG
                     EDAGAPDDPTLRSRAASAIREYAEAHSTLFERAERLRAKAERLERAGTPSESARNRAE
                     RARREVEAGLAALRASFAGGRDGHRAFDREVTLRYPAFEVSGENH"
     gene            complement(5106..5561)
                     /locus_tag="VFT03_05730"
     CDS             complement(5106..5561)
                     /locus_tag="VFT03_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847679.1"
                     /translation="MFGRKSRAERLKEQAEGSRLIPVATLAAAYAGARPVIERLLYDD
                     DLRDNLRVFITSARNILDELSGEEPSEIITKLWDDDKLRKQVEAAAEAAQEGSKRVRG
                     EKVRSGGGGRKLLLLLLVAAVGFLFFSPKTGPQVRKFAMEAYGSLTSGE"
     gene            complement(5631..>6715)
                     /locus_tag="VFT03_05735"
     CDS             complement(5631..>6715)
                     /locus_tag="VFT03_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEU4847680.1"
                     /translation="GKTIRTVTERLDAYDVTAAGRAIGDFVDELSNWYVRRSRRRFWK
                     GEDDLDKKAAHSTLYECLVAVTKLTAPFTPFVAESIYQNLVVNVDDDAPESVHLADWP
                     EAEDFMLDEALLSRMAAARRVVALGRAARNAAAIKTRQPLREVVVAPDFGDARFEESV
                     SSLQDIVLDELNVKELAFAEPGDVLSYDLKPNLVVVGPKYGRLVPGIRRALSEVPPEA
                     GERAAAGEAVTVDVEGQEITLPPEELLVEARGKEGYALERDRTLAVAVATELDEELAE
                     EGLVRELVHKVQNLRREKGFEIEEGIRVALSGSPRITALLDGRWGDYFRAEVLARELD
                     LENGAPDGESLSVDGEVIRVRLEPAG"
BASE COUNT         1072 a         2228 c         2231 g         1184 t
ORIGIN      
        1 agcgtcaacg aggcctgcgc cgtcggggtc gcagagggtg ccagggtcga ccccgggccg
       61 atcctcgcag cgctcgaagg gatgcccaac ggtttccgaa gctcgatgca gaaagacgtg
      121 gctgcggggc atccgccgga ggtcgatgcg atcgccggac ctatactccg tggcggtaga
      181 gagcacggca tcgacgtgtc cgccactcgt gccctcgtgg accggatcgt agcgccagag
      241 taaagaccag tgaaccatca caccaaccct cgaaacgatt ggtcctggca ggaagtcgag
      301 cgaggacgcg tctccgaaac aaaaagttga aatcctgaaa agcgagcgcg cgcaggatgc
      361 tgtatcgatc cgcattcgtc aggtcgcggg ttttcctatt ccggaaccgg cctccgtcgg
      421 cgtgaggtag cgttcgaacc actcgcacgc cagccgcgcc acctcttcca gagcgccggg
      481 ctcttcgaag aggtgcgtcg ctccggggac gatctcgagc tgcttttcgc agcggagcag
      541 agcgaacgcc ctccggttca gctcgacgac ctcgaagtcg tttccgccga cgatgagtag
      601 cgtgggcgcc cggacgcgtg ccagagctgg cccggccaga tcggggcgcc cgccgcgcga
      661 gacgatcgcg cccaccgcgt cggggcgccg cgccgccgct acgagcgccg cagcggcgcc
      721 ggtgctggcg ccgaagtagc caatacggag ctcgcgcgta tccgggtact gcgtgagcca
      781 gtccgtgacg ccaacgagcc gaccggcgag caggcggata tcgaagcgca ggtgagccgt
      841 tcgcagatcg accgcctctt cgtccggggt gagcaggtct accagcaggg tcgcgagctt
      901 cgcttcgttg agcacccgcg ccacgtagcg attgcggggg ctgtgcctgc tgcttccgct
      961 gccgtgggcg aagagcacga tcccacgggc cctctcgggc aggctcaggt tgccctcgag
     1021 cgtgaccgga cccgccgcga cgcggaccag ccgctcttcg cctctcggtt ccgctggtct
     1081 cattggagtt gccttctatg tgccggcttg aacccgtgtc tcggggtcgt ctacagctta
     1141 cactccgaac gggaacatcg agagtgcgcc ggtccaggct gacgcgctcg caaagacccg
     1201 gggccgcgtg gtaatgtcga gggccacgta caatgcggtg ggcgggagga tggcgagtca
     1261 tggatggtag caagatgctc aaggagattc gcgagcagcc ggaggtcttg ggacgggtgc
     1321 tcgacgaggg gtggggggag gttagcgccg cggcgcgcga gttgcgatcg cggggtttcc
     1381 gctccgcgat gctggccgcg cggggcacct cggataacgc tgctctctat gccaagtacc
     1441 ttttcgaggt gatcctgggc atcccgacgg ctctggcctc gccttcgacc ttcaccctgt
     1501 acgagaccga gatgaagctc gacgatgtcc tggtgatagg catcagccag tctggcgaga
     1561 gcacggacgt cctcgagacg gtccgaaggt cgggggagct gggtgccagt accctctccg
     1621 tgaccaacga cgaaggttct tcgatggccg ccgccgcaga gttccatttc tcactgcgcg
     1681 cgggcgagga gaagagcgtc gcggcgacca agtcctacac cgcggagctg ctcattctct
     1741 acctgctcgt gcgcgccctg aacggagagg aagagcctgg cgccgaggtt gggcgattgc
     1801 ccgaggcggc gcgggaggtg ctggaggcaa gctggaaagg gacggagcgc taccggtacg
     1861 ccgaccacct cgtggtcacc tcgcgcggct acaatttcgc gaccgccaag gaggcggccc
     1921 tcaagcttat ggagacgacc tacgtgctcg ccgaagcatt ctcggcagcc gacctcaggc
     1981 acgggcccat agccatgatc ggccaggact tccccgtgat cgccatagtt cctccaggca
     2041 aggtgcgcca ggggatggtc tccctcgtcg agagcctctg ggagaggggg gccgaggtgg
     2101 ccgtcataag cgacgacgag acgctggtcg acagggcggg ggtaggcttc gtagtgccgg
     2161 cttcgtgccc cgaagagctc tcgtccgtac tgtacgcgat cccaccccag atgctcgcgc
     2221 acgacctggc gcgcctgaag cggctcgacc ccgacgcccc gaggggcctg agcaaggtga
     2281 ccgagacctg gtaaacgagc ggttcatccg cgcggcgaag gctgagtgtg caaaatctct
     2341 aacctgatgg tgccggcaag gtaagatctt ggagtggaga acgaaagcga aaagaacggt
     2401 ctgcccttcg agttgggcgt cttcgacctc gacgggaccg tcttgcggcg ggacctcgag
     2461 atctcggaaa ggaccagggc cgccatggac gagctgcggc ggcgcggggt gcggctcgtc
     2521 gtggcgacgg ggcggcgttt cgagggagct cgcgagcact cagggaggct cggcttcgca
     2581 ggtaaagatc ccctgatctg cttcggcgga tcgatggtcc gccgcatgaa cggcgagacc
     2641 ctcctgcacc gcacgttgcc aggggacctt ggcgtcgagg tgctcgagtg ggcggcgggg
     2701 aggaacctgc acgcccgcgt tttcttggac gaacgcgtta tcacctctcc ggatactccg
     2761 gcggctttca aacacctgcg ccgttacgaa cagcccgggc tctctgtggt cgaggagccg
     2821 gcagactggc tccggaacgc cggggtggag ccgaccaagc tcgtgctcgt ggattaccca
     2881 gatgaagtcg aaggctggct cgaggaggcg caagatgcgt ttcgtggcag gctcttcgtc
     2941 acccgcagcc tgcctcatta cgtagaggtc gggagcatcg atggcaccaa gtccagagct
     3001 ttgcgcttcc tctgcgagcg ctggggggta gacccggcgc gcgcgctagc tttcggtgac
     3061 gcggacaacg acatagatat gctgcgcttc gccgggcacg gggtcgcggt gggggggatg
     3121 acggccgggg tgcgcgacgc cgccgacgcc gtcgcgccgc cggtgcacga ggacggcgtc
     3181 gccctctacc tcgagaagtt actgggtgac ctctagtgcc cgagctccct gaggtaacgg
     3241 tcatctcgga ggacatcagg tctctggcgg gcggacgcga ggtcttgcgg gccggggtct
     3301 tccgccctga cgtaaccaac gtggagccgg aggagttcac gaggaggctc gtcggacgca
     3361 cgctgcgcgg gacggggagg cgggggaaga tcacgctcct cgacttcggg gacgtgatcg
     3421 gggtcatcca cctggtcatc tccggacggg tcctgcgcct cccggcctgg acggagccgg
     3481 acaggatgaa caccgccgtc ctcgagttcg agggagggct cgtcctggcc ttcaccaggc
     3541 tctggctggg ctacttccac ctctacgagc cagggacgat agacgagcac cccctgatct
     3601 cacgcctcgg tcccgaccct ttctcggaag gcttcacccc agagtacctt gcgaaggctt
     3661 tcgaaagaaa ggcctctgtc aaagggcttc tcctcgacca gtcggtggtt gccggcctcg
     3721 gcaacatcta cgtggacgag gtgctattcg ccgccggcgt acatcccacc cgcaaggcca
     3781 acaccctggg tccggaagag atactcgcca tccacgccgc gacgcgcgac attctcggcc
     3841 gcgccatcga gctgcggggt acgaccttcg actcctacca cgacgcgttc ggcgagagcg
     3901 gcgagttcca gtaccagctc gaggtcttca cccgcgcggg cgagccctgc ccggtctgcg
     3961 gcacgaaagt agtcaaatca cgcgtcgcgg gccgccagac ctacacctgc cccgtttgtc
     4021 agcacgctcc ggcctagccc gctaggccgg agcgtgctat tcatccgtta tccggggcgt
     4081 ttccgctttt gggtgctgcc tcgatcagtt tcggcttgga gtcggcgaga tcctgggcct
     4141 ctccgtaggc cttctccgcc tcctcgtagc cggccccgat ctcatcgagg aggtcgaggg
     4201 ccttgtccct gttgttctcg agggcggggt cctcttcgag gaggtcgtag aactctactt
     4261 ccttggcttc ggcggcaagc tgggcgtcca tcgcctgtga gagcagtctc gcgtactgct
     4321 ttatgtcctt gtcgacgtcg aggtcctgca cggttgcgag ggattccctc gcgtcgtcga
     4381 ggttggcgcg cgcctcctcg agtgtgttct tggcctcggt aatgcggtcc ctctccgccg
     4441 aagggtcttc ccccgactct atctgctgct tgacctccgc gtaggtgtcg cgggccttct
     4501 cgaagagctt gttgtgctcg gctatcgact ggttcgcctc gacgatggca tcgttggcct
     4561 gctcgcgggg ggtctgaccg agcacgcccg agcaccctcc gaggaggagc gccgagaaag
     4621 agatcacgac gatgagcacg cgagggtata ttatctgtgc catggaccag caaagtttac
     4681 cgtttttcac gcaccttgcg ctgcgctcct tcccgggcac cctcaagcgc gcgctcggcc
     4741 gcttcttcaa gcgccgccgc ggggaggatg ccggagcgcc tgatgacccc acgctgcgct
     4801 cgcgggccgc ctcggcgatc cgcgagtacg ccgaagccca ctccacgctc ttcgagcggg
     4861 ccgagcgctt gagggcgaag gccgaacgcc tggagagggc agggacgccg agcgagagcg
     4921 cccgcaaccg cgcggagcgg gcgaggagag aggtcgaggc cggcctcgcc gccctccggg
     4981 cctcgttcgc cggcgggcgc gatggacatc gcgccttcga ccgggaggtc acgctcaggt
     5041 acccggcctt cgaggtctcc ggcgagaatc actgagcccg gcgtgcggca ggtgtccggc
     5101 cctgcctact cgcccgaggt caggctcccg taggcctcca tggcgaactt gcggacctgc
     5161 ggtccggtct tcgggctgaa gaagaggaag cccacggcgg ccaccagtag caagagcagg
     5221 agctttctgc cgccgccacc gctgcgcacc ttctcgccgc gcacccgctt gctgccctcc
     5281 tgggcggctt cggccgcggc ctcgacctgc ttgcggagct tgtcgtcgtc ccagagcttg
     5341 gtgatgatct ccgatggttc ttcgcctgag agctcgtcga gtatgttgcg ggccgacgtg
     5401 atgaagacac ggaggttgtc ccgaaggtcg tcgtcgtaga gcaggcgctc tatgaccggc
     5461 ctggccccgg cgtaggccgc cgccagcgtc gccacgggga tgagtcgcga cccctcggcc
     5521 tgctctttca gtctttccgc ccggctcttt ctcccaaaca ccgcaaaacc tccctgtcag
     5581 taggggtcgc ttttcgaggt tcctacatca tacccaactt cgccacgctt ctaccctgcc
     5641 ggctctagcc tgaccctgat gacctcaccg tcgacgctaa ggctctcgcc gtccggggcg
     5701 ccgttctcga ggtcgagctc ccgcgccagc acctccgccc tgaagtagtc gccccaccgt
     5761 ccatcgagca gagccgtgat gcgcgggctc cccgagagag ccaccctgat accctcctct
     5821 atctcgaagc ctttctcgcg cctgaggttc tgcaccttgt ggaccagctc ccgcaccagg
     5881 ccctcttccg cgagctcctc gtccagctcc gtggctaccg cgaccgcgag cgtgcgatct
     5941 cgctccagcg cgtacccctc cttgcccctg gcttcgacga gcaactcctc cggggggagc
     6001 gtgatctcct gcccttccac gtcaaccgtg accgcctcgc ccgcggcggc gcgctcgccc
     6061 gcctcaggcg ggacctcgga taaagccctg cggatgccgg gcacgaggcg gccgtacttc
     6121 gggcccacca cgacgaggtt gggcttgagg tcgtaggaga ggacgtctcc gggctcggcg
     6181 aaggcgagtt ccttgacgtt caactcgtcg agcacgatgt cctggaggct cgagacgctc
     6241 tcctcgaagc gcgcgtcgcc gaagtccggg gccacgacga cctcccgcag gggctggcga
     6301 gtcttgatcg ccgccgcgtt ccttgccgcc ctcccaagcg ctacgacccg gcgcgcagcc
     6361 gccatccgcg aaagcagcgc ctcgtcgagc atgaagtcct ccgcctccgg ccagtccgcg
     6421 aggtgtacgc tttcgggagc gtcatcgtcc acgttcacga cgaggttctg gtagatggac
     6481 tcggcgacga acggggtgaa aggggcggtg agcttcgtga cggccaccag gcactcgtac
     6541 agcgtggagt gggccgcctt cttgtctagg tcgtcctcgc ccttccagaa ccggcggcgg
     6601 ctgcgcctca cgtaccagtt cgatagctcg tccacgaagt ccccgatggc cctcccagcc
     6661 gccgtcacgt cgtaggcgtc gagcctctcc gtgacggtcc gtatcgtctt gccga
//
LOCUS       DASTUV010000285         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1038108, whole genome shotgun sequence.
ACCESSION   DASTUV010000285 DASTUV010000000
VERSION     DASTUV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038108"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFT03_05740"
     CDS             complement(<1..1075)
                     /locus_tag="VFT03_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4847681.1"
                     /translation="MTTAQTDHGTDGDGRTPGRPDPHDFLNVDAHLSEEEKEVRAGVR
                     AFVDDRIRPNIRDWFERAHFPRELVPEMGALGLLGMHLKGYGCAGKSAVVYGLACMEL
                     EAGDSGLRTFVSVQGSLAMSAIHKFGSEEQKERWLPPMARGEAIGCFGLTEPTAGSDP
                     ASMKSFARREGSEWILNGSKRWIGMGTIADVAVVWARTDEENNPVRGFLVPTDAPGFA
                     ARDITGKLSMRASVQCDLSFEDVRLPAGAMLPGAEGLKGPFACLNEARYGIIWGSMGA
                     ARDSFECALEYASEREQFGKPVAAFQLTQEKLVNMMLEVEKGTLLALHLGRMKDAGTL
                     RPEQISFGKLNNVREAIKIAREAR"
     gene            1153..1500
                     /locus_tag="VFT03_05745"
     CDS             1153..1500
                     /locus_tag="VFT03_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847682.1"
                     /translation="MDEAKNVPFGDMEWNDDAPGILAREAKVDGARWAIVRYGEGARR
                     EEWCEEGHRGYVVEGEIEYEFDDGREPLRASGGEAFLLPPATLGGGAHRGRNLAPGPT
                     RLFLIDDHPDKQA"
     gene            1497..2657
                     /locus_tag="VFT03_05750"
     CDS             1497..2657
                     /locus_tag="VFT03_05750"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU4847683.1"
                     /translation="MKLPLEGVRILDLSRVLAGPYTTMVLGDLGADVIKVEHPERGDD
                     TRHWGPPFTGGESAYYLSVNRNKRSISADLKEPEELEKIKQLAAGADVVIENMKRGAL
                     EKFGLGYEELREANPGLVYCSITGFGPGKDEQRPGYDFLVQARAGIMGITGFPEPEGQ
                     PTKVGVAIADIVCGLYAATAILAALRRRDATGEGARIEVPLFESTLGWLANRGQEYLV
                     SGEDTGRMGNGHPTIVPYQTFDASDKQIAVAVGNDAQFERLCEAIGREDLAQDERYAT
                     NPGRVANRQELVEALQQELSERTAEEWVEKIRGVGIPCGSVNTLAEALADEHLASTDM
                     LQEIEHPLAGVLEMLASPVLLDDERPPIRRPPPTLGQHNDEVDHNEWNGRNH"
     gene            complement(3009..>3197)
                     /locus_tag="VFT03_05755"
     CDS             complement(3009..>3197)
                     /locus_tag="VFT03_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847684.1"
                     /translation="GVFKSVCVSVTVAAIVGLRLEVDDPGAGARSVIVGAVMSTLKEY
                     EAACPMLPAESVAFTLKV"
BASE COUNT          614 a         1059 c          997 g          527 t
ORIGIN      
        1 tgcgcgcttc gcgcgcgatc ttgatcgcct cgcgcacgtt gttgagcttg ccgaaggaga
       61 tctgctccgg cctcaacgtc cctgcgtctt tcatgcgccc gaggtgcagc gccaggagcg
      121 tgcccttctc gacctcgagc atcatgttga cgagcttctc ctgggtgagc tggaaggcgg
      181 ccacgggctt gccgaattgc tcgcgctcac tcgcatactc taatgcgcac tcgaacgagt
      241 ctctcgccgc gcccatcgag ccccagatga taccgtagcg cgcctcgttg aggcacgcga
      301 aaggaccctt gagtccctcg gcgccgggca gcatcgcccc ggcggggaga cggacgtcct
      361 cgaaggagag gtcgcactgc accgaggctc gcatggacag cttgccggtt atgtctctcg
      421 ccgcgaagcc gggggcgtcg gttggaacga ggaagccgcg gacgggattg ttctcctcat
      481 cggtcctggc ccacacgacg gctacgtcgg cgatggttcc catcccgatc cagcgcttgg
      541 agccgttgag gatccactcc gagccttctc tgcgagcgaa ggacttcatg ctggcggggt
      601 cggagcctgc ggtaggctcg gtaaggccga agcagcctat ggcttcgcca cgcgccatgg
      661 gcgggagcca gcgctccttc tgctcctctg agccgaactt gtggatcgcg ctcatcgcca
      721 gcgacccctg caccgagacg aaggttctca accccgagtc tccggcctcc agctccatgc
      781 acgccagacc gtagaccaca gcgctcttgc ctgcgcagcc gtagcctttc aggtgcatcc
      841 cgagcagccc cagagcgccc atctcgggta cgagctcgcg cgggaagtga gccctctcga
      901 accagtctct tatgttgggc cgtatcctgt catcgacgaa ggcgcggacc ccggcccgga
      961 cctccttctc ctcctccgag aggtgggcgt ctacgttcag gaagtcgtga gggtccgggc
     1021 gtcccggcgt ccgcccgtcc ccgtccgtcc cgtggtccgt ctgcgctgtg gtcatggctt
     1081 tagcctctcc ttgtatccgc tgcgcgactg aattttcaca caaatacgaa gccgggtcgc
     1141 taaactacgg tcatggatga ggcgaagaac gtgccgtttg gcgatatgga gtggaacgac
     1201 gacgcgcccg gtatcctggc cagggaggcc aaggtggatg gggcgcgctg ggcgatagtc
     1261 aggtacggcg aaggagcgag gcgcgaggag tggtgcgaag agggccaccg cggctacgtc
     1321 gttgagggtg agatcgagta cgagttcgac gatggccgcg aacctttgcg ggcctccgga
     1381 ggcgaagcgt tcctgctccc gcctgccact ctgggcggcg gggcgcaccg gggtcgcaat
     1441 ctggcaccgg gtccgacgcg gctcttcctc atagacgacc atccggacaa gcaggcgtga
     1501 agctacccct cgaaggcgtg cggatcctgg atctctcccg ggttctggcc gggccgtaca
     1561 caacgatggt gcttggggac ctcggggcgg acgtgatcaa ggtcgagcac cccgagcgcg
     1621 gcgacgatac ccgccactgg ggaccgccct tcacgggcgg cgagtctgct tactacctgt
     1681 ccgtcaaccg taacaagcgt tcgatctccg ctgacctcaa agaaccagag gagctggaga
     1741 agataaagca gcttgctgcc ggtgcggacg tcgtgatcga gaacatgaag cgtggagcgc
     1801 tggaaaagtt cggtctcggc tatgaggagc tgagggaggc aaaccccggc ctcgtctact
     1861 gttccatcac cggcttcggg ccagggaaag acgagcaacg gcccggctac gacttcctgg
     1921 tccaagcgcg cgccggcatc atggggatca ccggcttccc cgagccggag ggccagccga
     1981 cgaaggttgg tgtcgcgatc gcggacatcg tctgcggcct ctacgccgcg acagcgatac
     2041 tggcggcgtt gcgccggcgc gacgctacgg gagaaggcgc gcgaatcgag gtgccgctct
     2101 tcgagagcac gctcggttgg ctcgccaaca ggggccaaga atacctggtc tcgggcgagg
     2161 acacaggccg catgggcaac ggccacccga cgatagtccc ctaccagacc ttcgacgcct
     2221 cggataagca gatcgcagtc gccgtcggca acgacgccca gttcgagaga ctatgcgaag
     2281 ctatcggccg agaagacctg gcgcaggacg agcgctacgc cacgaacccg ggccgggtcg
     2341 cgaaccggca ggagctggtc gaggcactgc agcaagagct ctccgaacgg acggcggaag
     2401 agtgggtcga gaagatccgg ggcgtaggta tcccctgcgg gtccgtaaac acgctcgccg
     2461 aagcgctcgc ggacgagcac ctggcgagca cggacatgct gcaagagatc gagcatcccc
     2521 tggccggcgt actcgaaatg ctcgcctcgc ccgtgctcct ggacgacgaa cggccgccga
     2581 tacggcgtcc tccaccgacg ctgggccaac ataatgacga agtcgaccat aacgaatgga
     2641 atggtcgaaa ccattgaatt ggtcagcatc gagatcttcg ccaaaaagct gctccatcac
     2701 cgtgctcgca caaactcctg aagagcgcac gggacgatca ccacaggcgt caaggacaag
     2761 acgggcaacg cgctcgcgac cgataaggtc tgggccttca ccatcgcaat actggctgac
     2821 accacgccac cgaccgtaac catcgactcc aagcctgccg ccatcacgaa caaccagaag
     2881 ccgatattcg gcttctccgc caacgagtcc aactcgacgt acgagtgctc gctctcgagc
     2941 gggactaatc ccgacgtctt tacgccctgc acctcgccga agacctacgg ccccctcgcc
     3001 gagggtacct acaccttcaa ggtgaaggcc accgactccg ccggcaacat cgggcaggcg
     3061 gcttcgtact ccttcaaggt cgacatcacc gcaccaacga tcaccgacct cgcgcccgcg
     3121 cctgggtcgt ccacctccaa ccgcagacca actatcgctg ccactgttac cgacacgcag
     3181 accgacttga acacacc
//
LOCUS       DASTUV010000286         4382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_827157, whole genome shotgun sequence.
ACCESSION   DASTUV010000286 DASTUV010000000
VERSION     DASTUV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827157"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFT03_05760"
     CDS             <1..814
                     /locus_tag="VFT03_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4847685.1"
                     /translation="QEVRHVYTPGTPFEVEALRGVSLAVEPDEVLGIVGGTGSGKSTL
                     VQHLNLLLEPTGGRVLVDGVDARTLRRSELRRRVGLVFQFPEAALFAPTVAEDVAFAP
                     RRLGMDEDEVRQRVRQTLELLGAGHLEQRSPFALSGGEKRRVAIAGVLAMAPEVLVLD
                     EPAAGLDPATREDLLGVVRALGDGGTSVVLVSHDLDEVAEVADRVYVLEEGRVRAVGT
                     PEEVFYAHLEASQAPTSVRVVAPLKKEYPEIGSPVRFEETLAALKGLLEGRR"
     gene            811..1602
                     /locus_tag="VFT03_05765"
     CDS             811..1602
                     /locus_tag="VFT03_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU4847686.1"
                     /translation="MTAGRTLGQFYPVGSPLHELDARAKILATGILAVGLFLVDSATG
                     LLLIAAAVVSLVVASRVPPGIFLDTLKPVALIVALTLLFQVFFSREGATLLEWGFLQV
                     HEGGLFRGLFLASRILLLVSTAALLTATTAPVELADGIEDLLSPLERLRFPAHELAMM
                     ITIALRFIPTLYEEAQKITRAQAARGADFAEGGPIRRARAMLPVLVPLTIGAFRRADE
                     LAEAMESRGYRGGEGRTRYRESRFRARDAIALATAVLVVVGGILL"
     gene            1599..2366
                     /gene="truA"
                     /locus_tag="VFT03_05770"
     CDS             1599..2366
                     /gene="truA"
                     /locus_tag="VFT03_05770"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU4847687.1"
                     /translation="MKKYAGLVEYDGSEFAGWAAQPGMRTVEGTFSEGLRTVLRHPVK
                     LSVAGRTDAGVHASGQVVSFETEGDLDPPQVAYKATAVLPTDLALRRCLETYEGFDAR
                     RDAKSRSYEYRIVNDPVRSPLKRRRATYVARELDFGLLKSAAELVRGTHDFRALTPAR
                     TYHVRFERIVTESAWEKSDDLLVYRVTADSFLYGMVRTLVGTMLEVADGRREMASFER
                     LLAGGERREAGQAVPSKGLTLVGVGYDNLDFRGGGIG"
     gene            2359..3036
                     /locus_tag="VFT03_05775"
     CDS             2359..3036
                     /locus_tag="VFT03_05775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU4847688.1"
                     /translation="MAETGNLGALLARIDDYIEERFAPQDEALEAALRESRRAGLPQI
                     QVSPNEAKLLQVLAEMVGARRILEVGTLGGYSAIHFGRALPEDGTLISLEIDERHAEV
                     ARKNVERAGLSSKVEIRVGDARELLARITENAEGPFDVVFIDADKEGYPEYLEWALKL
                     ARPGSLILGDNTIRSGSILDPQDDSARATSEFNERIAGDPRLSAILLPILRERVDGLT
                     IARVREG"
     gene            3112..3546
                     /gene="rplM"
                     /locus_tag="VFT03_05780"
     CDS             3112..3546
                     /gene="rplM"
                     /locus_tag="VFT03_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU4847689.1"
                     /translation="MMKSYMARPLEVERKWYVVDAEGQTLGRLATEIATILRGKNKPQ
                     YTPHVDTGDFVVVVNAEKVVVTGRKAEQKVYRRHSGYPGGLKETSYERMMERRPTEIL
                     RRAVKGMMPKNRLARQQLRKLKIYAGPEHPHAAQNPQPYEVR"
     gene            3549..3941
                     /gene="rpsI"
                     /locus_tag="VFT03_05785"
     CDS             3549..3941
                     /gene="rpsI"
                     /locus_tag="VFT03_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU4847690.1"
                     /translation="MAETLYRGTGRRKTARARVRLLPGDGRITVNGRDLDEYFTRPAY
                     RTAVRSPLELLGQANRYDVIAKIEGGGPTGQADALRHGIARALAEESQQARSELKSAG
                     LLTRDARAVERKKYGLKKARKRPQFSKR"
     gene            4030..>4382
                     /locus_tag="VFT03_05790"
     CDS             4030..>4382
                     /locus_tag="VFT03_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU4847691.1"
                     /translation="MQRRPITFGTDGARGVANEGLIPEDALRLGLAAARLFGGPILIC
                     RDTRLSGGMLSSALAAGAASGGARVIDAGVLPTPGVAALAPRLGAAAAGVVSASHNPY
                     PDNGIKFFSGDGRKLS"
BASE COUNT          727 a         1420 c         1548 g          687 t
ORIGIN      
        1 ccaggaggtg cggcacgtct acacgccggg gacccccttc gaggtcgagg cgttgcgggg
       61 cgtctcgctc gcggtcgagc ctgacgaggt gctcggcata gtcggcggga cgggctcggg
      121 gaagagcacg cttgtccagc acctgaacct gctcctcgag cccacgggcg gccgcgtcct
      181 cgtggacggc gtggatgcgc ggacgctcag gcggagcgag ctgcgccgca gggtgggcct
      241 cgtcttccag ttccccgagg ccgcgctctt cgccccgacc gtcgcagagg acgtggcctt
      301 cgccccgcgc cgcctcggaa tggatgaaga cgaggtacgg cagcgggtac gccagacgct
      361 ggagctgctc ggagccggac acctggagca gagatctccg ttcgcgctct ccggcggcga
      421 gaagcggcgc gtcgcgatag cgggcgtgct cgcaatggct cccgaggtac tcgtcctcga
      481 cgagccggct gccgggctcg accccgccac ccgcgaggac ctgctcggcg tcgtgcgcgc
      541 cctcggggat ggtggcacct cggtcgtgct cgtctctcat gacctcgatg aggtcgccga
      601 ggtcgccgac agggtgtacg tgttggagga gggtagagta cgggcggtag ggacgccgga
      661 ggaggtcttc tacgctcacc tcgaagcttc gcaggctccg acgagcgtgc gggtggttgc
      721 gcctttgaag aaggagtacc ccgagatcgg gagcccggtc cgtttcgaag agacgctggc
      781 ggcgttgaag ggtctgctgg agggtaggag atgacggcgg gccggaccct cggccagttc
      841 tatccggtgg gctcgccgct ccacgagctg gacgcgcggg ccaagatcct ggcaaccggg
      901 atacttgctg tcggcctctt cctcgtcgac tccgcgaccg gattacttct gatcgccgcg
      961 gcggtcgtct ccctcgtggt ggcgagccgg gtgccgccgg ggatcttcct cgacactctg
     1021 aaacccgtgg cgctcatcgt ggcgctcacg ctgctttttc aggtcttttt ctcccgggag
     1081 ggagcgacgc tcttggagtg gggcttcctc caggttcacg agggcggcct cttcaggggc
     1141 ctctttctcg cttcgaggat actgctgcta gtctcgacgg cggcgttgct cacggcgacg
     1201 acggcgcctg tggagctggc tgacgggata gaggatctgc tctccccgct cgagcgactc
     1261 cgtttcccgg cgcacgagct ggcgatgatg atcacgattg cgctccgctt catccccacg
     1321 ctctacgagg aggcgcagaa gatcacccgc gcccaggcgg cccgcggcgc ggacttcgct
     1381 gagggcggcc ccattagacg cgcccgcgcg atgctgcccg tcctcgtacc gctcaccata
     1441 ggcgccttcc gcagggccga cgagctagcg gaggcgatgg agagtcgcgg ttacaggggc
     1501 ggcgagggcc ggacgcgcta ccgcgagtcg cgcttccgtg cccgcgacgc catcgccctc
     1561 gccaccgccg tgctcgttgt ggtagggggg atcctgcttt gaagaagtac gccggcctcg
     1621 tggagtacga cggctccgag ttcgcaggct gggccgccca gcccggcatg aggacggtcg
     1681 agggcacgtt ctccgagggg ctgcgcaccg tgctccgcca ccccgtgaag ctcagcgtag
     1741 cagggcgcac cgacgccggc gttcacgcca gcggacaggt agtctccttc gagaccgaag
     1801 gggacctgga cccgccgcag gtcgcgtaca aggccacagc ggtcctcccc acggacctcg
     1861 ccctcaggcg ctgccttgag acctacgagg ggttcgacgc ccgaagagat gcgaagagca
     1921 ggagctacga gtaccgtatc gtcaacgacc ccgtccgctc ccccctgaag aggcggcggg
     1981 ccacctacgt cgcaagggag ctcgacttcg gcctcctgaa gagcgccgcg gagctcgtcc
     2041 ggggcaccca cgacttccgg gccctcaccc cggcgaggac ctaccacgtc cgcttcgagc
     2101 ggatcgttac cgagtccgcc tgggagaaga gcgacgacct gctcgtctac agggtgacgg
     2161 ccgactcctt cctgtacggc atggtccgca cgctggtcgg cacgatgctc gaagtggcgg
     2221 acggaaggcg cgagatggca tccttcgagc ggctgctcgc cggaggggag cggcgcgagg
     2281 ccggccaggc ggtcccgagc aagggcctca cgctggtggg cgtgggatac gacaacctgg
     2341 acttcagagg aggcggaatt ggctgagacc gggaacctgg gcgcgttgct cgcccggata
     2401 gacgactaca tcgaggagag gttcgctccg caggacgagg cgctcgaggc cgccctgcgg
     2461 gagtcgcggc gcgccgggct gccgcagata caggtctctc ccaacgaggc gaagctcttg
     2521 caggtgctcg ccgagatggt gggagcgagg cgtatcctcg aggtggggac tctgggcggc
     2581 tacagcgcca tacacttcgg gcgcgcgctg cccgaggatg gaaccctgat ctccctcgag
     2641 atagacgagc gccacgcgga ggtcgctcgc aaaaacgtcg agcgcgcagg gctgagctcg
     2701 aaggtcgaga tccgggtcgg cgacgccagg gagctcctgg cgcggatcac cgagaacgcc
     2761 gaaggaccct tcgacgtcgt cttcatagac gcagacaaag agggctaccc cgagtatctt
     2821 gaatgggctc tgaagctcgc gcgccccggc tcgctcatcc tcggggacaa cactatcagg
     2881 agcggcagca tcctcgaccc ccaggatgac tcagcgcggg cgacaagcga gttcaacgag
     2941 cgtatagccg gagatccacg gctatcggcg atcctcttgc ccatactccg cgagcgcgta
     3001 gacgggctca ccatagcgag ggttcgggag ggctgagaag ctgttacttt ggggtatgta
     3061 tgctaacatc ttacggtttg atacttgttt agctgttaat cgttccggag gatgatgaag
     3121 agttacatgg cgcggcccct ggaggtcgag cggaagtggt acgtcgtaga cgccgagggg
     3181 cagaccctcg ggcggctcgc gacggagatc gccacgattt tgcgcggcaa gaacaagccg
     3241 cagtacacgc cgcacgtcga caccggcgac ttcgtggtcg tggtgaacgc cgagaaggtg
     3301 gtagtcacgg gcaggaaggc cgagcagaag gtctacaggc ggcactccgg ctaccctggc
     3361 gggctcaaag agacgagcta cgagcggatg atggagcgca ggcccaccga gatcctgcgc
     3421 agggccgtca aggggatgat gccgaagaac cggctcgccc gccagcagtt gaggaagttg
     3481 aagatctacg cgggacccga gcatccgcac gctgctcaga acccgcagcc ctacgaggtg
     3541 aggtgagcat ggcggagact ctgtacaggg ggaccggacg gcgcaagacg gcgcgcgcgc
     3601 gtgtgaggct gctcccaggg gacggcagga tcacggttaa cggccgtgac ctcgacgagt
     3661 actttacccg cccggcttac cggacggcag ttcgctcgcc gctcgagttg ctcggccagg
     3721 ccaaccgcta cgacgtgatc gccaagatcg agggcggcgg tcccacgggg caggccgacg
     3781 cgttgcggca cggcatcgcg cgggcactgg ccgaggagtc gcagcaggcg cgcagcgagc
     3841 tcaagagcgc ggggctcctc acccgggacg cccgggccgt cgagcgcaag aagtacggtc
     3901 tcaagaaggc ccgcaagcgc ccgcagttct ccaagcgcta ggacggcaaa ccttttacag
     3961 gagacgggcc cctttcgccg cttcttcggg cggctcgaga ggggcccgtc tcgcgccgag
     4021 gaggtctatt tgcaaagaag accgatcact ttcggcaccg acggggcgcg tggggtcgcg
     4081 aacgagggcc ttatccccga ggacgcgctg aggcttggcc tcgccgccgc aaggctcttc
     4141 ggaggcccta tcctgatctg ccgcgacacc cgcctctccg gggggatgct ttcttccgcc
     4201 ctcgccgcag gcgcggcgag cggcggggcg cgggtgatcg acgccggggt tctgcctacc
     4261 cccggcgtcg ccgccctcgc gccccggctc ggagcggccg cagcgggcgt ggtgagcgcc
     4321 tcgcacaacc cctacccgga caacggcata aagttctttt ccggcgatgg ccggaagctc
     4381 tc
//
LOCUS       DASTUV010000287         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1013989, whole genome shotgun sequence.
ACCESSION   DASTUV010000287 DASTUV010000000
VERSION     DASTUV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013989"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1248
                     /locus_tag="VFT03_05795"
     CDS             169..1248
                     /locus_tag="VFT03_05795"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU4847692.1"
                     /translation="MADFAQSPSRNRIAAIGGDGIGPEVIESGIRVLEAATEGDSASP
                     LSFTRFPWGCEYYLQHGQMMPEDALETLAGFDAIYLGAVGWPTVPDHVSLWGLLIPIR
                     RGFDQYVNLRPARLLRGVESPLAKPGSLDVTVVRENTEGEYSDSGGRLFSGTPHEIAV
                     QDSVFTRRGVERILRYAFEEAKGRRGLLTGATKSNGISITMPFFDEIFREVGEDYPDV
                     EASLMHADALAARLVLDPERFDVVVGSNLLGDILSEITAAVSGAIGIAPSANLNPERK
                     YPSLFEPIHGSAPDIAGEGVANPAGAIWAGALMLEHLGHPKLGERVLSALEETLASGT
                     KTRDLGGSASTAEVTEPVIERLQSR"
     gene            1410..2141
                     /locus_tag="VFT03_05800"
     CDS             1410..2141
                     /locus_tag="VFT03_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU4847693.1"
                     /translation="MGGIAGLTIFLGLPLARLRFESLRYLSLLNAFAIGILLFLFIEV
                     MEHAIAPVEEAIEEGEPGFLLLLVVVAGGFAVGLLSLVYYAQHFLRGESSAQRLAILI
                     AAGIGLHNFSEGLAIGSSARAGEMALALTLVVGFGLHNATEGFGIAAPLASEKRVSWG
                     FLAVCALIAGGPTFLGTAVGYSYTSELMSVLFLALSGGAIIYVVNELFGVGRKLGAPA
                     WAGWGITAGILLGFVTEFLLEALGG"
     gene            complement(2159..>2618)
                     /gene="folE"
                     /locus_tag="VFT03_05805"
     CDS             complement(2159..>2618)
                     /gene="folE"
                     /locus_tag="VFT03_05805"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU4847694.1"
                     /translation="YAYLFAGLSEDPTRHLDVGFMESARDTVLVRDIPLASLCEHHLL
                     PFIGKAHLGYAPDGRVVGLSKLARLVEGYARRPQLQERLTAQIADALYDSLGSRGSIV
                     VIEADHSCMTMRGVQKPGSITVTSAVRGIYEEDHSLRAETMALISRGSIP"
BASE COUNT          449 a          820 c          841 g          508 t
ORIGIN      
        1 gagatcaaga aggatcgggg cgaagcagcc ccgatccgat accccctcgg aaccacacgg
       61 ctagttcgat atcattcggc ctcctggccc ggcttgacat tggatcgcgc cacgccgggt
      121 tgctagcatg gatccaggag gatggatcac cgaccaacta ggagagcttt ggcagacttc
      181 gcacagtcac ccagcaggaa caggatcgcc gccatcggcg gggatgggat cggacccgag
      241 gtcattgaat ccggcatccg ggtcctcgag gccgcgacgg agggagacag cgcttcacct
      301 ttgagcttca ctcggttccc gtgggggtgc gagtactacc tgcaacacgg acagatgatg
      361 ccggaagacg ccctggagac cctcgcgggt ttcgacgcga tctacctcgg agccgtgggc
      421 tggccgacgg tccccgatca cgtctccctt tggggcctcc tcatccccat caggcgtgga
      481 ttcgaccagt acgtcaacct gcgcccggcc aggcttctgc gcggcgtcga gagcccgctg
      541 gcgaagccgg gctcgctaga cgttaccgtg gtaagagaga acacggaggg tgagtactcc
      601 gactcgggcg ggcgtctgtt cagtggcacg ccgcacgaga tagccgtcca ggacagcgtc
      661 ttcacccgcc gtggggtcga gcggatcttg cgctacgcct tcgaggaggc aaagggccgc
      721 cgcgggctcc tcacgggggc gacgaagtcc aacggcatca gcataacgat gccgttcttc
      781 gacgagatct tccgcgaagt cggcgaggac tacccggacg tcgaggcctc cctcatgcac
      841 gccgacgccc tggccgcccg gctcgtgctc gaccccgagc gcttcgacgt ggtcgtcggc
      901 tccaacctcc tcggcgacat cctctcggag atcacggcag ccgtctccgg ggccatcggg
      961 atagcccctt ctgcgaacct gaaccccgag aggaaatacc cttcgctctt cgagccgatc
     1021 cacggttccg cccctgacat cgccggcgag ggcgtcgcca accccgcggg tgcgatctgg
     1081 gccggcgccc tgatgctcga acacctcggc cacccgaagc tcggagaacg cgtgctctcg
     1141 gcgctcgaag agacgctcgc atcgggaacc aagacccgcg acctcggcgg ctccgccagt
     1201 acagcagagg tgacagaacc tgtgatcgaa cgcctccaga gccgctgaac ttcgcggtat
     1261 ttgatctagc tcacaaattc ctcacctacg ccttgactcg cactcgccgc ctaccttact
     1321 ctacgtagtt actgggaatg attgtcattt gtaggaagcg atagttagca gggggtgacg
     1381 gcactttgtc gtttttgcag acggtgctct tgggcggtat agctggtctc accatctttc
     1441 ttgggctgcc gctggcgcgg ctcaggttcg agtccctgcg gtatctgtcg ctcctcaatg
     1501 cattcgccat agggattttg ctgtttcttt tcatcgaggt gatggagcat gctatcgcgc
     1561 ctgtcgagga ggcgatagag gaaggtgagc cggggttcct gctgctgttg gtggtggtag
     1621 cggggggttt cgctgtgggc ctgttgagcc tcgtgtatta cgcgcagcat tttctgcgag
     1681 gtgagagctc ggcgcagcgg ctagcgattc tgatcgccgc tggcataggg ctacacaatt
     1741 tttccgaggg tctcgccata ggcagctcgg cgcgggcagg tgagatggcg ctggcgctga
     1801 cgctggtcgt gggcttcggg ttgcacaacg ccacggaggg attcggcatc gccgctcccc
     1861 tggcctcgga gaagagggtg agctggggct ttctcgccgt ctgcgcgctc atcgccgggg
     1921 ggccgacctt tctgggtacc gccgtgggtt attcttacac ttcggagctt atgtcggttc
     1981 tcttcctcgc cctctccggc ggtgcgatca tctacgtcgt caacgagctg ttcggggtgg
     2041 ggcgcaagct cggcgcgccc gcctgggccg gctggggcat aaccgcaggg atactgctcg
     2101 gtttcgtcac ggagtttttg ctggaggccc tcggcggcta accgttcggg taccccggct
     2161 aaggtatgga accgcgtgag atcagggcca tagtctccgc gcggaggcta tgatcctcct
     2221 cgtagatccc gcgcaccgcc gaggtgaccg tgatactgcc cggtttctgg acgccgcgca
     2281 tcgtcataca cgagtggtcg gcctcgatca cgacgatgga gccacgagag cccagactat
     2341 cgtagagggc gtcggcgatc tgggccgtga gacgttcctg gagctgaggc cgtcgcgcat
     2401 acccctccac gagccgcgcc agcttgctga gaccgacgac ccgtccgtcc ggagcgtacc
     2461 ccagatgagc cttgccaata aaaggcagga gatgatgctc gcacaggctc gccagcggta
     2521 tgtcgcgcac caggaccgta tctcgcgcgg actccatgaa acccacatcc aggtggcggg
     2581 tgggatcttc gctcaggccc gcaaaaaggt acgcgtag
//
LOCUS       DASTUV010000288         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_790567, whole genome shotgun sequence.
ACCESSION   DASTUV010000288 DASTUV010000000
VERSION     DASTUV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790567"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFT03_05810"
     CDS             complement(<1..755)
                     /locus_tag="VFT03_05810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU4847695.1"
                     /translation="MSEKNCSFEHLEGHRYARLTTYRKSGEPVPITVWFAEVGVPDNR
                     VYAFTDALSGKAKRIRNDPRVTLAPSTFRGRPRGEELRAEARVLDRAEHEVADRVLRE
                     KYGWQYRLALAVIRLLGLSARQAFLEMRPAASGETAPVRPTRYSGITVHDSNPVKSYL
                     QRRRLRDALSVLDDLGEDFAGRFLDFGGGSGELSRILAGRFPRAKVFCYEPSPVLLKE
                     ARQNLSSFKNVVVLPSLPRLKNKKFRYVFCLEV"
     gene            complement(752..1312)
                     /gene="rfbC"
                     /locus_tag="VFT03_05815"
     CDS             complement(752..1312)
                     /gene="rfbC"
                     /locus_tag="VFT03_05815"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEU4847696.1"
                     /translation="MNVFETGLPGVVIIEPDVFGDDRGFFMETWNGRRYEEAGLPGRF
                     VQDNLSYSARGVLRGLHFQNPQPQGKLVSVLRGEVFDVAVDIRVGSPTFGEWTGVTLS
                     AENKRQFYVPPEFAHGFLVTGEDALFFYKCTDYYAPSAEGIVLWNDPEIGIEWPADAP
                     TLSGRDSEAPPLREMPKGILPRYQSG"
     gene            complement(1309..2190)
                     /gene="rfbA"
                     /locus_tag="VFT03_05820"
     CDS             complement(1309..2190)
                     /gene="rfbA"
                     /locus_tag="VFT03_05820"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU4847697.1"
                     /translation="MRGIVLAGGSGTRLYPLTLAVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRDILVISTPHDLPRFEELLGDGSQWGVNFAYAEQPQPEGLAQAFVIGREFVGADNV
                     SLVLGDNIFYGQGFGALLESAVSRKEGATVFGYYVKDPERYGVVEFDGDGTVLSIEEK
                     PKSPRSHYAVTGLYFYDNQILDIASSLEPSGRGELEITDANLAYLYRDQLRVEVMGRG
                     MAWLDAGTHESLMQSANFIETLELRQGLKIGCPEEIAYSKGYIDAGELQRLAHPMSKN
                     SYGQYLLDLLRNPETPV"
     gene            complement(2187..>2730)
                     /locus_tag="VFT03_05825"
     CDS             complement(2187..>2730)
                     /locus_tag="VFT03_05825"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU4847698.1"
                     /translation="TYGLPTLTTNCSNNYGPYQFPEKLIPLMIYNALRGEPLPVYGDG
                     RQVRDWLYVKDHCRAVVRVLEAGTVGEVYNVGGHNERTNLDVVKTICGLLDELVPDSP
                     HAPHESLITFVEDRPGHDRRYAIDAGKIERELGWTPDETFESGMEKTVRWYLRNRVWC
                     EGVLSGSYRGERLGLGGRRS"
BASE COUNT          462 a          894 c          874 g          500 t
ORIGIN      
        1 aagacttcca gacagaacac atagcggaac ttcttgttct tcagcctcgg cagcgaggga
       61 aggacgacca cgttcttgaa gctcgagagg ttctgcctcg cttctttgag cagaaccggg
      121 gacggctcgt aacagaacac tttggcacgc gggaaccgtc cggcgaggat cctgctcagc
      181 tcgccactcc cgcccccgaa gtccaggaac ctgcccgcga aatcctcgcc gaggtcgtcg
      241 agcacagata gagcgtcacg caggcgccgc cgctgcagat agctcttgac ggggttactg
      301 tcgtggacgg tgatacccga gtagcgcgtg ggccgaaccg gtgcggtctc cccgctcgcg
      361 gcgggccgca tctcgaggaa agcctgccgc gcggagaggc ccagcagccg gatcaccgcc
      421 agggccagcc ggtactgcca cccatacttc tcgcgcagca ccctgtcggc cacctcgtgc
      481 tcggcccggt cgagaacgcg agcttcggcc ctgagctcct cgccccgagg acgtcccctg
      541 aacgtactcg gggcgagggt cacccgcggg tcattgcgga tgcgcttcgc cttcccggac
      601 agggcatccg tgaaggcata gacgcggttg tcaggtaccc cgacctccgc gaaccagacc
      661 gtgataggaa cgggttcccc gctcttgcgg taggtggtca ggcgcgcgta ccggtggcct
      721 tcgaggtgct cgaaggagca gtttttctca ctcatcccga ctggtagcgg gggaggatgc
      781 ccttgggcat ctcgcgcaac ggtggagcct cgctatctct cccggagagc gtcggcgcgt
      841 cggcaggcca ctcgatccct atctccggat cgttccagag gacgatccct tcggcggagg
      901 gggcgtagta gtccgtgcac ttgtagaaaa agagtgcgtc ctcgcccgtt acgaggaagc
      961 cgtgggcgaa ttcagggggc acgtagaact ggcgcttgtt ctccgcagag agcgtcacgc
     1021 ccgtccactc tccgaacgtg ggggagccga ccctgatgtc taccgcgacg tcgaagacct
     1081 ccccccgcag gacggagacg agcttgccct ggggctgggg gttctggaag tggaggccgc
     1141 gcagcacgcc gcgggcggag taggagaggt tgtcctgcac gaagcggccg ggcaggccgg
     1201 cctcctcgta gcgcctcccg ttccaggtct ccatgaagaa gccgcggtcg tctccgaaca
     1261 cgtcaggctc gatgatcacg acgccaggga ggccagtctc gaagacgttc ataccggtgt
     1321 ctccgggttc ctgagcaggt cgagaaggta ctgcccgtag ctgttcttgc tcatcggatg
     1381 cgccagcctc tggagctctc cggcatctat gtagcccttg ctgtaggcga tctcctcggg
     1441 gcaccctatc ttcaggccct gcctgagctc aagggtctcg atgaagttcg ccgactgcat
     1501 cagggattcg tgggtccccg cgtctagcca cgccatgccc ctccccatga cctcgacgcg
     1561 gagctggtcg cggtagaggt aggccaggtt ggcgtcggtg atctccagct ccccccttcc
     1621 cgagggctcg aggctcgagg ctatgtccag gatctggttg tcgtagaagt agaggcccgt
     1681 gaccgcgtag tgggagcgag gactctttgg tttctcctcg atgctcaaga ccgtgccgtc
     1741 cccgtcgaac tcgacgaccc cgtatcgctc ggggtccttg acgtagtagc cgaagacggt
     1801 agcgccctcc ttccggctca ccgcgctctc gagtagcgcc ccgaagccct gtccgtagaa
     1861 gatgttgtcc ccgaggacca gagagacgtt gtccgccccc acgaactcac gcccgatgac
     1921 gaaggcctgc gccaggccct ccggctgtgg ctgctcggcg taggcgaagt tgacgcccca
     1981 ctgggagccg tcgccaagga gctcctcgaa gcgcgggagg tcgtggggtg tggagataac
     2041 cagtatgtcc ctgatgccgg cgagcatgag ggtggagaga gggtagtaga tcatcggctt
     2101 gtcgtagacc ggcaggagct gcttgctgac ggccagagtg agcgggtaga gccgggtccc
     2161 cgacccgccc gcgagcacga tccccctcac gagcgccgcc ctcccaggcc caacctctcg
     2221 cctcgatagc tacccgagag cacgccctcg caccacaccc gattcctcag gtaccagcgc
     2281 acggtcttct ccatgccgct ctcgaaggtc tcgtccgggg tccagccgag ctcgcgttct
     2341 atcttgcccg cgtctatggc gtagcgcctg tcgtggccgg gccggtcctc gacgaaggta
     2401 attaatgact cgtgcggcgc gtgcggggag tccggaacca gctcgtccaa caacccacat
     2461 atagtcttca ccacatcgag gttcgtccgc tcgttgtggc cgccgacgtt gtacacctcg
     2521 cccaccgtcc ctgcctcgag cacccgcacc acggcccggc agtggtcttt gacgtagagc
     2581 cagtccctga cctggcgccc gtcgccatag acaggaagcg gctcgccgcg cagggcgttg
     2641 tagatcatta gcggtatcaa cttctctggg aactggtagg gtccgtagtt gttggagcag
     2701 ttggtcgtga gggtcggtag cccgtaggtg
//
LOCUS       DASTUV010000289         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_783769, whole genome shotgun sequence.
ACCESSION   DASTUV010000289 DASTUV010000000
VERSION     DASTUV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783769"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..2172)
                     /gene="fusA"
                     /locus_tag="VFT03_05830"
     CDS             complement(25..2172)
                     /gene="fusA"
                     /locus_tag="VFT03_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4847699.1"
                     /translation="MAVQVVKKTPLRRVRNIGIMAHIDAGKTTTTERILLYTGLTHRL
                     GEVHDGNAVMDWMSQERERGITITSAATTVFWGGIEGSEKNGKGTKEGLHSRIPEQHR
                     INIIDTPGHVDFTVEVERSLRVLDGAIALFDSVAGVEPQSETVWRQADKYGVPRIAFV
                     NKMDRIGADFYRAVETMHERLGANAVPVQLPIGAESEFEGIVDLVEMKAIIYKDDLGA
                     EWEETEIPAGMRDLAEEYRENLLEAIADQDEDLMVMYLEGEEIEPDQIRAAIRKATLE
                     IEMTPVFVGSAFKNKGVQPLLDGVIDYLPSPLEVPPIQGITPDGEEVTREADENGPLA
                     ALAFKVQSDPHVGKLTYMRVYSGTLKAGSYVMNTTKDRRERVGRLLQLHANSREQRDE
                     VYAGELVAAIGLSNTGTGDTLISADDDERIVLEEMVFPEPVISQAIEPRTKADQEKLS
                     TALQRLAEEDPTFTVRGDEETGQTIIAGMGELHLEIILDRLLREFRVDANIGRPQVAY
                     RETVRRRAEDVEGRFVRQTGGRGQYGHVVINMEHNEEGGYEFENKIVGGVIPREYIPS
                     VDKGILEALDSGVVAGYPVVDVRVELVDGSYHEVDSSEMAFQIAGSMAAREALKRANP
                     TLLEPIMDVEVVVPEEFMGDVMGDLSARRGHIQGMDSRGGGQVIRAMVPLSEMFGYAT
                     TVRSKTQGRATFTMQFDHYAEVPKSIAAEISEG"
     gene            complement(2186..2656)
                     /gene="rpsG"
                     /locus_tag="VFT03_05835"
     CDS             complement(2186..2656)
                     /gene="rpsG"
                     /locus_tag="VFT03_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU4847700.1"
                     /translation="MPRRAAPPKRKIPADPVYGSTMVQQLINKIMLDGKKGTAEKIVY
                     DALSFVEERTGSNPVTVLNNALDNIRPRLEVRSRRVGGATYQVPVEVPPRRANTLALR
                     WMVGFARARREKTMGRRLAGEILDAKDNIGSSVKRKEDTHRMAEANRAFAHYRW"
BASE COUNT          400 a          917 c          784 g          558 t
ORIGIN      
        1 aatactcctc ttgacataag gtctctatcc ctccgatatc tcggccgcga tgctcttggg
       61 cacctcggcg tagtggtcga actgcatggt gaaggtggcg cggccctggg tcttggagcg
      121 cacggtggtc gcgtacccga acatctcgga gagcggcacc atcgcgcgga tgacttgccc
      181 acctcccctc gagtccatac cctggatgtg gccacgccgc gccgagaggt cgcccatgac
      241 gtcacccatg aactcctcgg gcacgactac ctcgacgtcc attatcggct cgagcagggt
      301 cgggttggcc cgcttgaggg cctccctggc cgccatgctc cccgctatct ggaaggccat
      361 ctccgaggag tcgacctcgt ggtaggagcc gtcgaccagc tctaccctga cgtcgactac
      421 cggataaccg gccacgacgc ccgagtcgag cgcctccagg atgcccttgt ccacgctcgg
      481 gatgtactcc ctggggataa cgccgccgac gatcttgttc tcgaactcgt agccgccctc
      541 ttcgttgtgc tccatgttga tcactacatg gccgtactgg ccgcggccgc cggtctgcct
      601 gacgaagcgt ccctcgacgt cctctgcccg ccgcctgacg gtctcgcggt aggctacctg
      661 cggcctgccg atgttcgcgt ctacacggaa ctcgcgcagc agcctgtcga gtatgatctc
      721 caggtgcagc tcgcccatcc cggcgatgat cgtctgcccc gtctcctcgt cgccccgcac
      781 ggtgaaggtc ggatcctcct cggcgaggcg ctgcaaggcc gtggatagct tctcctgatc
      841 tgccttggtc ctcggctcga tggcctgcga gatcaccggc tccgggaaga ccatctcctc
      901 gagcacgatc cgctcgtcgt cgtcggccga gatcagagta tcgccggtgc ccgtgttcga
      961 gagcccgatg gccgcgacca gctcgcccgc atagacctcg tcgcgctgct cccggctgtt
     1021 ggcgtggagc tgcaggagcc tgccgacgcg ctcgcggcgg tccttggtag tgttcatcac
     1081 gtaggagcca gccttgagcg tgcccgagta caccctcatg tacgtcagct tgccgacgtg
     1141 cgggtcggac tggaccttga aggccagagc cgccagaggc ccgttctcgt cggcctcgcg
     1201 ggtgacctcc tcgccgtccg gcgtgatgcc ctggatcgga ggcacctcca gcgggctcgg
     1261 caggtagtcg atgacgccgt caagcagcgg ctggacgccc ttgttcttga acgccgaccc
     1321 gacgaagaca ggagtcatct cgatctcgag cgtcgctttg cggatggccg cgcggatctg
     1381 gtcgggctcg atctcctcgc cctccagata catgaccatg aggtcctcgt cctggtcggc
     1441 gatagcctcg agcaggttct ccctgtactc ctcggcgaga tcccgcatcc cggccggtat
     1501 ctcggtctcc tcccactcgg ccccgaggtc gtccttgtag atgatcgcct tcatctccac
     1561 aaggtccaca atgccctcga actcggactc ggcccctatc ggcaactgca caggaaccgc
     1621 gttggccccg agcctctcgt gcatggtctc tacggccctg tagaagtcag ccccgatcct
     1681 gtccatcttg ttgacgaacg cgatcctggg taccccatac ttgtcagcct gccgccagac
     1741 cgtctcactc tgcggctcga ctccagcaac cgagtcgaac agcgctatcg caccgtcgag
     1801 cacccgcagg ctccgctcca cctccacagt gaagtccacg tgcccgggcg tgtctatgat
     1861 attgatacgg tgttgttcgg ggatacggct atgaaggccc tccttggtgc ctttgccgtt
     1921 cttctccgag ccctctatgc cgccccagaa cacggttgtg gcggcggaag taatagttat
     1981 gccgcgctcg cgctcctggg acatccagtc cataacggcg ttaccgtcgt ggacctcgcc
     2041 cagcctgtgg gtaaggcctg tatagaggag gatccgttcg gttgtcgtcg tcttccccgc
     2101 gtcgatatga gccatgatcc cgatgttccg gacccggcgt agcggggtct ttttaactac
     2161 ctgtacagcc attgaacttt cctcgtcacc atctgtagtg tgcaaacgcc cggttggcct
     2221 ctgccatccg gtgggtatct tccttgcgct tcacgctaga gccgatgttg tccttcgcgt
     2281 cgaggatctc gcccgcgagc ctgcggccca tcgtcttttc gcgtcgggcg cgggcgaagc
     2341 cgaccatcca gcgcagggcg agggtattcg ctctccgcgg gggaacctcg accggcacct
     2401 ggtaggtcgc gccgccgacc cggcggctcc gcacctcgag gcgcggccgg atattgtcga
     2461 gggcgttgtt caggaccgtc accgggttcg agccggtgcg ctcctcgacg aaggaaaggg
     2521 cgtcgtagac tatcttctcc gccgtgccct tcttaccgtc gagcatgatc ttgttgatca
     2581 gctgctgcac catcgtgctc ccgtagacgg gatcggcggg aatcttcctc ttcggcggcg
     2641 ctgctcgcct cggcatcta
//
LOCUS       DASTUV010000290         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_977003, whole genome shotgun sequence.
ACCESSION   DASTUV010000290 DASTUV010000000
VERSION     DASTUV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977003"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VFT03_05840"
     CDS             <1..1267
                     /locus_tag="VFT03_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847701.1"
                     /translation="TGPAIVTFPGVWEEYLRRWDGLAGSDGEAPEIAGLRLKRLPEVG
                     TYYHGGAVCSLPVPEGHPWREAWERFAGVHGGLGPEITRLLTTDWHEPELRPALVRLL
                     RLYGTRPTTRSYLDSLGWLPDRLREILAIHTLNAGIGPSRTPALYASMPAVMATEGVW
                     VPEGGVYEIARALERLARSSGVELKTGEPVLEIRPGKVFTSEEQYTADAVVSGLDPER
                     LSRLLEGGWKRPAGRLSCSGIAMYAAIRGELPARLSTHGVVLPSDPAALYASLEAVEE
                     PDETMAFVDYYRPGGPYPNRGHVLALGLTAPANGREYGLEDAFVAREVERVSRVIGLP
                     KPATEYFGEHEILHPRYFGAWGSAGGALYGAVKPFWMTGPLHRPRYSDRKRPWLWRVG
                     ASVHPGGGIPAVLGGAMISSSKLLGFLGK"
     gene            1302..2441
                     /locus_tag="VFT03_05845"
     CDS             1302..2441
                     /locus_tag="VFT03_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU4847702.1"
                     /translation="MNLGEQRDLFEIPEEISYLNCAYMSPQLRSAREVGERAVARKSR
                     PWEITPEDFFEDAEKSRELFARLAGGDADGVALVPSVSSGIAVAAANVPVREWQKILL
                     LEDQFPSNVYSWRELAQRSGADLVTVPRPADHGWTSAVLDHLDENTAVVAVPNCHWTD
                     GSLLDLVSIRERAREAGAALVVDAIQSLGAHPFDARAVRPDFLVASAYKWLLGPYGVG
                     FMYAAEEWRDGTPIEHNWINRRGTEDFSQLVEYEDSFQPGARRYDVGERSNFALLPMA
                     NEALRQILDWGVENVSETIGGLTDLIEREAGDLGIEAVPAKSRARHMVGLKLGSAAPE
                     DLAARLAAEGVFVSVRGNSMRVSPHVYNTEEDVERLFEVIGRTIA"
     gene            2577..>3370
                     /locus_tag="VFT03_05850"
     CDS             2577..>3370
                     /locus_tag="VFT03_05850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU4847703.1"
                     /translation="MLAEIGRTADESGFASLWVMDHFFQIRGVGEPGEPMLESYSALS
                     FLAGMTQRAKLGTLVTGVIYRHPGILVKTVTALDVLSGGRAYLGIGTGWNEREALGLG
                     VPFPSTRERFERLEETLRISKQMWSGEVAPYEGKHYRLEEPLNSPQALSRPHPPIMIG
                     GMGERRTLRLVAHYADACNLFAHGGADLIRHKLSVLRGHCEDVGRDYEEIERTALGTV
                     KLGRDGMTEDEVIGTCRELNEAGIQHLIFNMPNVHEIRPLEAFGER"
BASE COUNT          581 a         1057 c         1209 g          523 t
ORIGIN      
        1 cacgggcccc gccatcgtca ccttcccggg ggtctgggaa gagtacctga ggcgctggga
       61 cgggcttgcc ggaagtgacg gcgaagcccc tgagatagcg ggcctcaggc tgaagcggtt
      121 gcccgaggtg ggcacctact accacggggg cgctgtctgc tcgctgcccg tgcccgaggg
      181 acatccgtgg cgggaagcgt gggagcggtt cgccggagtc cacggggggt tggggccgga
      241 gatcactcgc ctcctgacga cggactggca cgaaccagag ctccgtcctg cgctcgtgcg
      301 cctgctccgt ctctacggga cgagaccgac aaccaggagc tacctggaca gcctcgggtg
      361 gctgcctgat aggttgaggg agatcctcgc catccacacc ctcaacgcgg gtataggacc
      421 ttctcgaacc cccgcgctct acgcaagcat gccggcggtc atggcaacgg aaggggtgtg
      481 ggtgcctgag gggggagtat acgagatcgc gcgggcgctc gagaggctcg cccgttcgag
      541 cggcgtggaa ctgaagacgg gggagcctgt tctcgagatc aggcccggga aagtgttcac
      601 ctcggaagag caatacaccg ccgacgcggt agtgagcggg ctcgaccccg agcggctgag
      661 caggctcctc gaaggcggct ggaagagacc ggctgggagg ctctcctgct cggggatagc
      721 tatgtacgcg gccatccggg gcgagttgcc tgcgcgtctc tcgactcacg gcgtcgtgct
      781 cccctcggac cccgccgcgc tgtacgcgag cctggaggcg gtggaggaac ccgacgagac
      841 tatggctttc gtggactact accggcccgg cgggccgtac ccgaaccggg ggcacgtgct
      901 ggcgctgggg ctgaccgccc cggcaaacgg ccgggagtac gggctcgagg acgccttcgt
      961 cgcgcgggag gtagagcggg tgtcgcgtgt gatcggactc ccgaagccgg ccacggagta
     1021 ttttggcgaa catgagatcc tgcacccgcg gtacttcggc gcctggggaa gcgccggcgg
     1081 ggcgctctac ggtgcggtca aacccttctg gatgaccggg ccgctgcacc gcccgcggta
     1141 ctcggatcgc aagaggccct ggctgtggcg ggtcggcgct tcggtacacc ctggcggtgg
     1201 gataccagcg gtccttggcg gcgccatgat ctcttccagc aagctgctcg ggtttctggg
     1261 taaataagag ggcaggcgag cacggaaagg aaccagtcgg catgaacctc ggggagcagc
     1321 gcgacctgtt cgaaataccg gaggagatct cctacctcaa ctgcgcctac atgtcgcccc
     1381 agcttcgctc ggcgcgagag gtcggcgagc gggcggtggc gcgcaagtcc cggccctggg
     1441 agataacgcc cgaggacttc ttcgaggacg ccgagaagtc cagggagctg ttcgcgcggc
     1501 tcgccggcgg ggatgcggac ggcgtcgccc tcgtcccgtc cgtcagctcc ggtatcgccg
     1561 tcgccgcggc taacgtcccc gtgcgggaat ggcagaagat actgttgctg gaggaccagt
     1621 tcccctcgaa cgtctactcg tggcgcgagc tcgcgcagcg cagcggcgcg gacctggtga
     1681 cggtcccccg ccccgccgat cacggctgga cctcggccgt gctcgaccat ctcgacgaga
     1741 acaccgccgt cgtcgccgtg ccgaactgcc actggacgga cggatcgctg ctcgacctcg
     1801 tcagcattag ggagcgcgct agggaggccg gggctgcgct cgtggtggac gccatacagt
     1861 cgctcggggc tcacccgttc gacgcgcgcg cggtacggcc ggacttcctg gtcgcctccg
     1921 cgtacaagtg gctgctcggg ccgtacgggg tcggcttcat gtacgccgcg gaggagtggc
     1981 gggacgggac gcccatagag cacaactgga tcaaccgccg cggcaccgag gacttctcgc
     2041 agcttgtgga atacgaggac tccttccagc ccggcgcccg acgctacgac gtcggcgagc
     2101 ggagcaactt cgccctgctc ccgatggcga acgaggcgct gcgccagatc ctcgactggg
     2161 gcgtcgagaa cgtctccgag actatcggag ggctgaccga cctcatcgag agagaggccg
     2221 gggatctcgg catcgaagcg gtaccggcga agagtcgggc gcggcacatg gtcgggctca
     2281 agctcggctc ggccgccccc gaagacctcg ctgcccggct cgccgcagag ggcgtcttcg
     2341 tgagcgtgcg cggcaacagc atgcgcgtct cgccacacgt ctacaacacc gaggaggacg
     2401 tagagcgcct cttcgaggtt atagggcgta cgatagcttg acgctcgttc ctttctcgta
     2461 ccggaagata gaatctgccg gtcgggcgtt gtgggagcga gaggggagag ggagtgcgta
     2521 tcgggctaca gattccgagc ttcacctggc cggacggagt cggggggata ggtcccatgc
     2581 tggccgagat cggcaggacc gcggacgaat ccggtttcgc gagcctctgg gtcatggacc
     2641 acttcttcca gataagaggc gtcggcgagc ccggggaacc gatgctcgaa agctacagcg
     2701 ccctgagctt tctcgcaggg atgacgcaaa gggcgaagct cggcacgctt gtgaccggag
     2761 tcatctaccg ccaccccggc atcctggtca agacggtcac cgcgctcgac gtgctctcgg
     2821 gtggaagggc gtatctcggc atcggcactg gctggaacga gcgtgaggca ctgggtctgg
     2881 gcgtcccgtt tccgtccacg agggagcgct tcgagcgcct ggaggagacg ctccggatct
     2941 cgaagcagat gtggtcgggg gaagtcgcgc cctacgaagg caagcactac cggctggagg
     3001 agccgctgaa cagcccgcag gctttgagcc gtccgcaccc gccgatcatg atcgggggga
     3061 tgggcgagag gcggacgctg cggctcgtgg cgcactacgc cgacgcctgt aacctcttcg
     3121 cgcacggcgg cgcggacctg atccgccaca agctcagcgt cctgcgggga cactgcgagg
     3181 acgtcggacg cgactacgag gagatcgaac gcaccgctct gggcacggtg aaactcggga
     3241 gggacggcat gacggaggac gaggtgatcg ggacctgccg cgagctgaac gaagcgggca
     3301 tacagcacct catcttcaac atgcccaacg tccacgagat aaggccgctc gaggcgttcg
     3361 gagagaggat
//
LOCUS       DASTUV010000291         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_388925, whole genome shotgun sequence.
ACCESSION   DASTUV010000291 DASTUV010000000
VERSION     DASTUV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388925"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1043)
                     /gene="trpD"
                     /locus_tag="VFT03_05855"
     CDS             complement(18..1043)
                     /gene="trpD"
                     /locus_tag="VFT03_05855"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU4847704.1"
                     /translation="MLRDALRKAAAGEHLTEGEAERALETIMEGTVPPAATAALLTAL
                     RVRGEAVSEIVGFARAMRRFSARVEAPEDVVDTCGTGGDAKGTINVSTAAAFVARGAG
                     VTIAKHGNRAATSQAGSADVLEALGAEIELGPEQVGECIEESGIGFMFARTHHPAMRF
                     VAPVRAELPFRTVFNLLGPLTNPAGARRQVVGVFGGAYVRPVAEALRGLGAEKALVVH
                     GMDGMDEITVTDRTLVAEVEDGDIEEYEIAPEDFGLARHAPDGLLGGDAHLNARILRD
                     VLSGEEIGAARDVVLLNAGAAVYVSGKAKTVEQGVRLSETSIASGKALAALEDFVHTT
                     RRIGGIG"
     gene            complement(1049..1651)
                     /locus_tag="VFT03_05860"
     CDS             complement(1049..1651)
                     /locus_tag="VFT03_05860"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU4847705.1"
                     /translation="MLGCGLSAKILDIMRVLVIDNYDSFTYNLVQYLGELGAEVLVRR
                     NDEVTPEEVLELHPDRIVVSPGPCTPKEAGVSVELIEKVSGQIPILGVCLGHQAIGQA
                     FGAEIVRGEPVHGKTAKILHDGEGVYRGVQQGFEATRYHSLVIEPESLPEYLLVTSRT
                     EDGTIMGVRHRDFPVEGVQFHPESVLTRSGHELLKNFLEV"
     gene            1664..2614
                     /locus_tag="VFT03_05865"
     CDS             1664..2614
                     /locus_tag="VFT03_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEU4847706.1"
                     /translation="MTLILTESEVGELLDLPSTLDAVEAVLRDQAAGKATNRARRRVA
                     LPKSSLNYMAAGAPGIGALGLKAYSVARTGARFYTMLFDPESGELLSILQSDRLGQMR
                     TGAASGVATKHLAREDATTLGLYGAGWQAESQLEAIAAVRDLTRVIVHSRSEDSRKQF
                     AEKMSERLGMEVETTHSADEPAAQDIVVTATSAKEPVLLGEWLRPGAHVNAAGSNFLF
                     KSEIDREVVRRASFVCVDSREELGLEAGDLLNPLETGLILPEAIYELGQVIAGQVGGR
                     EGPEDITLFASQGLALEDLAAARVVYDRAVERDVGREIEF"
BASE COUNT          478 a          964 c          889 g          448 t
ORIGIN      
        1 gaggacggtg gcgcggctca ccctatgccc ccgatacgtc gtgtcgtgtg aacgaagtcc
       61 tcgagcgccg cgagagcctt gcctgacgcg atggacgtct ccgaaaggcg cacgccctgt
      121 tcgactgtct tcgctttccc cgagacgtaa acggcggcgc cggcgttaag gaggaccaca
      181 tcccgggcgg ccccgatctc ctcgccggag aggacgtcgc gcaggatacg tgcgttcagg
      241 tgggcatcgc cgccgaggag gccgtcgggg gcgtggcggg cgaggccgaa gtcttcgggg
      301 gctatctcat actcctcgat gtccccatcc tccacctcgg cgactagggt cctgtcggtg
      361 accgtgatct catccatccc gtccatcccg tgcaccacga gggccttctc tgctccaagc
      421 ccccgcagcg cctcggcaac aggccggacg taggcaccgc cgaagacgcc gacgacctgg
      481 cgcctcgccc ctgcggggtt ggtgagcggc ccgagcaggt tgaagaccgt gcggaaaggc
      541 agctcggccc tcacgggggc tacgaacctc atcgccgggt ggtgtgtgcg ggcgaacatg
      601 aatccgatgc ctgactcttc tatacattcg ccaacctgct ccggccccaa ctctatctcg
      661 gcgccgagcg cttcgaggac gtctgcgctg ccggcctggc tcgtggcggc tctgttgccg
      721 tgcttggcta tggttacgcc cgcaccgcgc gccacgaaag cggcggccgt ggagacgttg
      781 atcgtaccct tcgcgtcacc acccgtgccg caggtgtcaa cgacatcctc gggcgcctcc
      841 accctggcgg aaaagcgtcg catcgcgcgg gcgaagccga ctatctccga gaccgcctca
      901 ccgcgcaccc tcaaagccgt gaggagcgcc gccgtcgcgg ccggaggcac tgtcccctcc
      961 atgatagtct cgagcgcccg ctcggcctca ccctcggtca ggtgctcccc ggccgcagcc
     1021 ttgcgcaggg catcgcgcag catccccgct agacctcgag gaagttcttc aacagctcgt
     1081 gcccgctgcg ggtcagcacg ctctcgggat ggaactggac gccttcgact gggaagtcgc
     1141 gatgcctgac gcccatgatc gtgccgtcct cagtgcgaga ggtgaccagt aggtactctg
     1201 gcaaggactc gggctcgatg acgagcgagt ggtagcgggt cgcctcgaag ccctgctgga
     1261 cccctctgta gacgccctcg ccgtcgtgca ggatcttcgc cgtcttgccg tgcacgggct
     1321 cgccgcgcac tatctcggcc ccgaatgcct gtccgatcgc ctggtggccc aggcagacgc
     1381 caaggatagg gatctggccg gagaccttct cgatcaactc caccgagacg ccggcctcct
     1441 tcggcgtgca cggaccgggc gagaccacta tcctgtcagg gtggagctcg aggacctctt
     1501 ccggcgtgac ctcgtcgttg cgccggacga gtacctccgc gcccagctcc ccgaggtact
     1561 ggaccaggtt gtacgtgaag gagtcgtagt tgtctatgac caggacccgc atgatgtcga
     1621 ggattttagc cgataacccg caccccaaca tcgaggaggc gccttgaccc tgatcctgac
     1681 cgaaagcgag gtcggggagc tactcgacct gccctcgacc ctcgacgccg tcgaagcggt
     1741 cctgcgcgac caggccgcag ggaaagcgac taaccgcgcc cgccgcaggg tcgccctacc
     1801 gaagagcagc ctgaactaca tggccgccgg agctcctggc atcggggctc tcgggctcaa
     1861 agcctactcc gtcgcccgca ccggcgcccg tttctacacg atgctcttcg atcccgagag
     1921 cggggagctg ctctcgatcc tccagtcgga caggctcggc cagatgcgaa ccggcgccgc
     1981 gagcggcgta gccaccaagc acctcgcccg cgaggacgcg acgactctgg gtctctacgg
     2041 ggcggggtgg caggcagaga gccagctcga agcgatagcc gcggtcaggg accttacgcg
     2101 cgtcatcgtc cacagccgca gcgaggactc gcgcaagcaa ttcgcagaga agatgagcga
     2161 gaggctcggc atggaggtag agaccaccca ctcggccgac gagccagcag cccaggacat
     2221 cgtcgtcacc gcgacctcgg cgaaggagcc ggtcctgctc ggcgaatggc taaggccggg
     2281 agcgcacgtc aacgccgcag gatccaactt cctgttcaag agcgagatcg accgcgaggt
     2341 cgtcaggcgc gcgagcttcg tctgcgtcga ctcgcgcgag gagctcgggc tcgaagccgg
     2401 cgacctcctc aaccccctcg agacaggtct cattctcccc gaggccatct acgagctcgg
     2461 ccaggtcata gcgggacagg tcggaggccg cgagggaccc gaggacataa ccctcttcgc
     2521 cagccagggc cttgccctcg aggacctggc tgcagcccgc gtggtctacg accgggccgt
     2581 cgagcgcgac gtaggccgcg aaatagagtt ctgagaagta gcttgtcagc acgctccggc
     2641 ttcgcctccg cttctcagca cgccgccttg ggcttcgcct caggcggctt ctcagcttat
     2701 tctctctgtg cacagagaga ataagctgag aagccgcctg aggcgaagcc caaggcggcg
     2761 tgctgagaag cggaggcga
//
LOCUS       DASTUV010000292         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1777276, whole genome shotgun sequence.
ACCESSION   DASTUV010000292 DASTUV010000000
VERSION     DASTUV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777276"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..387
                     /locus_tag="VFT03_05870"
     CDS             166..387
                     /locus_tag="VFT03_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847707.1"
                     /translation="MFNDWHYVLSQDREAREQDFAERVAERGRYARSPGRPSLRGRVA
                     RRLFALAIAAESEETWRVVWERLEARGRL"
     gene            429..1223
                     /locus_tag="VFT03_05875"
     CDS             429..1223
                     /locus_tag="VFT03_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4847708.1"
                     /translation="MKALELRGVEKSFKKRRGEVVRALDSVSLSVGKGEVVGILGPNG
                     SGKSTLVRVISTLVVPDRGEVEVFGIDATRHPKRVQRSMNRVSVEASFFKKLSAMENL
                     LYGAKLYGVTSAEARPRIKEILDNIGFARKRATEPMEHLSRGMQQKIALARALLTSPV
                     VMLLDEPTTGLDPRSKKDVQEFIRKIREAHDSSILLCTHDMGEAEELCDRVGIMMDGK
                     ILALEESEELKRRYTTNGRVPTMEETFMAATGYSVEEASAEGDQDA"
     gene            1216..2058
                     /locus_tag="VFT03_05880"
     CDS             1216..2058
                     /locus_tag="VFT03_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847709.1"
                     /translation="MHNMLKPRWLVEVIAVWGYVQRNYFLTKRYFMWELVWLTFTTVN
                     AMSITFIGAGVELVGGGEGTDTERLMTFLLIGALIWSYLSMIFDVLSETVQWERWEGT
                     IEYTFMSPTSRVTHLLGMGVYAVVYGIARTAVVLGVAMLAFDLDLSEANYWAAMVILA
                     VCSISLVGFGVVAAVMPLLSPEKGQQVTYIVSALLLLVSGVYYPVKVLPDWMQAIAQY
                     SPVTYALEGSRAALQDGVGLSHLWGSIWPLLVMGAVFVPMGLYVFHLGESYAKRTGKL
                     KRSG"
BASE COUNT          421 a          643 c          718 g          390 t
ORIGIN      
        1 ttctagctcg actacacttc tcaggagagg cccccggacc gagcggaccg ggggcctcga
       61 gctttgctcc gagagagctg gcgaaggtct ttggctccga tcccaacacg gacggtatca
      121 gcccttccag ggcgcgacga agagacgaga gaaggagaca gagagatgtt caacgactgg
      181 cactacgtgt tgtcacagga ccgggaagcc cgtgagcaag acttcgccga aagggtggcc
      241 gagcgcggga gatacgctcg gtcccctgga cggccctccc taaggggaag ggtagcgcgc
      301 aggctcttcg ccctggcgat agccgccgag agcgaggaga cctggcgcgt cgtctgggag
      361 aggttggagg ccagggggcg cctgtagagc gggcgcccct cagggcggga tttctacagg
      421 aggttcctat gaaggctctg gagttaagag gagtagagaa gagtttcaag aagcggcgtg
      481 gggaggtggt acgcgccctc gactcggtct cgctctcggt cgggaaaggg gaggtcgtcg
      541 gcatcctcgg gcccaacggg agcggaaaga gcacgctggt gcgagttatc tccacgctcg
      601 tcgtccctga caggggggag gtcgaggtct tcgggatcga cgccacgcgc catcccaagc
      661 gggtgcagcg cagcatgaac cgggtaagcg tcgaggccag cttcttcaaa aagctctcgg
      721 ccatggagaa tctgctctac ggggccaagc tctacggcgt caccagtgcc gaggcgaggc
      781 ccaggataaa ggagatcctc gacaatatcg gcttcgcccg caagcgtgcc accgagccga
      841 tggagcactt gagccgaggg atgcagcaga agattgcgct cgccagggcg ctgctcacca
      901 gccccgtcgt gatgctcctc gacgagccga cgaccggcct cgaccccagg agcaagaagg
      961 atgtgcagga gtttatccgc aagatacgcg aggcccacga ctcctcgatc ttgctctgca
     1021 cccacgacat gggcgaggcc gaggagctct gcgaccgcgt cggtatcatg atggacggga
     1081 agatactggc cctcgaagag tcagaagagc tcaaacgccg ctacaccacg aacgggcggg
     1141 tgcccacgat ggaggagacg tttatggccg cgacgggcta ctccgtcgaa gaggccagtg
     1201 cggaaggaga tcaggatgca taacatgctc aagccccgat ggctggtcga ggtaatcgcc
     1261 gtctggggct acgtacaacg caactacttc ctcaccaagc gctactttat gtgggagttg
     1321 gtgtggctga cgttcaccac cgtcaacgcc atgtccatca ccttcatagg ggctggtgtc
     1381 gaactggtcg gcggcggtga agggacagac accgagcgtc tgatgacctt cctgctcatc
     1441 ggcgcgctga tctggagcta cctctcgatg atcttcgacg tgctctcgga gacggtgcag
     1501 tgggagcggt gggagggaac gatagagtac acgttcatga gcccgacgag ccgggtcacc
     1561 caccttctcg gcatgggcgt ctacgccgtc gtctacggca tcgcccggac cgcagtggtc
     1621 ctcggcgtcg ccatgctcgc cttcgatctc gacctctcgg aggccaacta ctgggccgcc
     1681 atggtcatcc tcgccgtgtg cagcatctcg ctcgttggtt tcggggtcgt agccgccgtg
     1741 atgccgctgc tctcgccgga gaagggccag caggtgacgt acatcgtctc ggccctcctg
     1801 ctcctcgtct cgggcgtcta ctaccccgtc aaggtgctcc ccgattggat gcaggcgata
     1861 gcgcagtact caccggttac ttacgcgctc gaaggatccc gcgcagcgct ccaggacggt
     1921 gtcggcctct cacacctgtg gggcagcatc tggccgctac tcgtcatggg cgcggtattc
     1981 gtgcccatgg gactctacgt cttccacctc ggcgagagct acgccaagcg gaccggaaag
     2041 ctcaagagga gcgggtgata gaggtgtcag gttacaggct tcaggtgtca gctaattgct
     2101 ggtggctgat gcctgtggcc ttgttacaga ccctcgtaga tctccgccag ggatagatct
     2161 gtctccggac ag
//
LOCUS       DASTUV010000293         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1553559, whole genome shotgun sequence.
ACCESSION   DASTUV010000293 DASTUV010000000
VERSION     DASTUV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553559"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..808
                     /locus_tag="VFT03_05885"
     CDS             191..808
                     /locus_tag="VFT03_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847710.1"
                     /translation="MRDRYRRPDGTRWNGQQLQDATGGVVTRSYVSMMRKGRIENPGF
                     DKLRAMAKAMGFPPESWFEESDGNRMAARLDPEDAKRDVASRLNHLFEIVRNERTGEP
                     YSSADIARMSLGDLTEDQIEEIRTGTLENPAISQVIALAEAFGVHPSYFLETGRRPHL
                     LGEQEMNALGDSRARAILTKSLNLSDREKDMVLDMIQHLGNLHDR"
     gene            1103..1402
                     /locus_tag="VFT03_05890"
     CDS             1103..1402
                     /locus_tag="VFT03_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847711.1"
                     /translation="MTVMVTHPPLEDISLEEAPDLFVEEVLSGERTAPDIYEVPVAEV
                     EHLERETESYLAWVVVLGFVAYAIVLYWAHKCERRGGDASIKYSILKGFKVRCVK"
     gene            1481..2251
                     /locus_tag="VFT03_05895"
     CDS             1481..2251
                     /locus_tag="VFT03_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4847712.1"
                     /translation="MSGLRVSGATKRYGGKTVVSGLSLTVEPGMVAGLVGPNGCGKTT
                     TLGMIAGLITPDEGRISVSGYDTVVSALDARRSLGFVPDTPSGFEHLSVREYLELYRN
                     LYSQPPDRYEERAGVILEAFGLLEYGEASLGELSHGIRRKVATTAALSLDVPVILIDE
                     PTAGLDPEGVVVLRRLVRHIGVAGSAVLLATQDLAFAERVCDQLYLLYEGGLVADGSP
                     DLLRERYRASSIEETFLAVTGMERRIDDFERRLDAFSR"
     gene            2265..3644
                     /locus_tag="VFT03_05900"
     CDS             2265..3644
                     /locus_tag="VFT03_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847713.1"
                     /translation="MRRLRPVARSSPVLALLAPAGLIAGLAWAGWRGAASMAASNDGA
                     STAVVLTVGIVFAIVGFNVQHVSSAGRSLDAQVRSAPLSRLGLFMGTVGIPFAICCLV
                     VSILSLALFVPLIYAAEAPLYAPIDLALFQAAVFYAAGALGEVLKRVTRRQPAALLAG
                     PPLVLAWVASGHATGGGAWPGIARPLGHTVLNTTTEPALRLTGALLLLLLAGVGVWIS
                     VAAFLGLPEQQTFSHVGRQLRAPGSGSGVVSSVALKRMVRDRSVQRHTLFVALVAGTL
                     SCLTSALLPDVAQAAVGGLLVLAALSVAIVPLATYGANRDSSWFWRSTPVSFAAYVLG
                     MVGAGICAGTVVVVAPTAAALPFFWVGREPPELAVVVVVVVVILLVATGAGFLVPCSL
                     ENTSEQILSYAAFGASLAGVFAAGSWVAPRLTVLAIPESLVVVGLTLVVAGLVVAAAF
                     LRERERRKV"
     gene            3641..4510
                     /locus_tag="VFT03_05905"
     CDS             3641..4510
                     /locus_tag="VFT03_05905"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEU4847714.1"
                     /translation="MIFDVEVPAFPQAVLLQRTHLLDTVTGDRVSLNETAYFMLSLVD
                     GRRTAKDIGEAVAAVYGLAPRQVTSDFLQIAALLNEKCLLNFDTPLRSRCATFPRTVR
                     LFLFDVALGQLRFPWRRKRLDFCNESRRIGLLSVVRHLSLPAAMMGAPLSLAVWLLLG
                     EVMSSFWIAAGIALGFAVSLILHEAAHAVALAPVPAFLSLYGPVFLVGHPEIPPGKGL
                     LVSAAGPAIAGLTGLLLMLASCVLRSEPLVFAGEILSMNLLGMTTVAADGRKALRNLA
                     RVLDPPERRSAKC"
BASE COUNT          745 a         1411 c         1513 g          925 t
ORIGIN      
        1 tatagccatc gtagtagcct cctcaatact tcccaacgaa cagacgcctc cgagcggatt
       61 gtactcgaaa cgcccacctt ctcgtctatt cgggtggtgt tgattaaacg gtacggaaca
      121 tggtatcgta tttacaacaa agtggtgaga ggcaggtctg cggttgcgca tctcccagaa
      181 atttgaacag ttgcgggacc ggtaccgccg gccggacggc accagatgga acggccagca
      241 gctccaggac gccacgggcg gggtggtgac gcgctcgtac gtgagcatga tgcgcaaggg
      301 taggatagag aaccccggct tcgacaagct gcgggccatg gcgaaggcca tgggcttccc
      361 gcccgagtcc tggttcgaag agtcggatgg gaacaggatg gccgcgaggt tggatcccga
      421 ggatgcaaag cgagacgtcg ccagccggct gaaccacctc ttcgagatcg tcaggaacga
      481 acgcaccggc gagccctact ccagcgccga catcgcccgc atgagcctcg gggaccttac
      541 cgaggaccag atcgaggaga tcagaaccgg gacgctcgag aaccccgcca taagccaggt
      601 aatcgccctc gcggaggcgt tcggcgtcca cccttcctac ttcctcgaga cgggcaggag
      661 gccgcacttg ctcggggaac aggagatgaa cgcgcttggc gacagcaggg cgcgcgccat
      721 cctcaccaag agcctgaacc tctcggaccg ggagaaggac atggtgctcg acatgatcca
      781 acacctcggt aacctgcacg accggtagta gatccacacc tcaaatcccc accggaacct
      841 ccgctacgcc ccaagcacgc tcgacctctg gctcacccct gaacacgaga tgtacgtaaa
      901 gtaatctgtc cttacgtaca gtaaatttga caaagcttat gttttggcgt aacgtgtcgt
      961 tctgagttgg gaggttgtcg cggtgggcag agacggttag ttcgatgatg ggatcgggtg
     1021 acgcgtcgtg gaaggctgtt cgggctcctc ctcgatctgg agtgtacgag gggtgatcgg
     1081 gaacgggaag gaggtaggcg gtttgacggt aatggttact catcctcctc tggaggacat
     1141 ttcgctggag gaagcgccgg atctcttcgt agaggaggtt ctctccggag agcgcacggc
     1201 acccgacatc tacgaggtcc ctgtcgcaga ggtcgaacac ttagagcgcg agaccgagtc
     1261 ttaccttgcc tgggtcgtcg tactgggttt cgtcgcctat gcgatcgtgc tgtactgggc
     1321 gcacaagtgc gagaggagag gtggagacgc cagcataaag tacagcatcc tcaagggctt
     1381 caaagtcagg tgcgtcaagt agcggcgttg gcagtgcgaa tacacatctc cggtccgcgc
     1441 gtttacgcgc ggaccggggg gcggtagagg gggcaggccc ttgagcggcc ttcgggtcag
     1501 cggcgcgacg aagcgatacg gaggcaagac ggtcgtgagc gggctctccc tgaccgtcga
     1561 gccggggatg gtcgccggcc tcgtcgggcc gaacgggtgt ggcaagacca ccaccttagg
     1621 tatgatcgcc ggtctcataa caccggacga ggggaggata agcgtctcgg gttacgatac
     1681 cgttgtttcc gctctggacg cgaggcgcag cctcggcttc gtccccgata ctccgagcgg
     1741 tttcgagcat ctcagcgtgc gcgagtacct ggagctctac cggaacctgt atagccagcc
     1801 gcctgaccgg tacgaggaac gggccggggt catactcgag gccttcggac ttctggagta
     1861 cggcgaggcc agcctcggcg agctctctca cggcatccgt cgcaaggtcg ccacgaccgc
     1921 cgccctgtcg ttggacgtgc cggtgatcct gatcgacgaa cccacggcgg gtctggaccc
     1981 cgaaggggtg gtggtactgc ggcgtctcgt gagacacatc ggagttgccg gttccgcggt
     2041 tttgctcgcg acccaggatc tcgccttcgc cgagcgcgtg tgcgaccagc tgtatctcct
     2101 ctacgagggt gggctcgttg cggacggaag ccctgatctg ttgcgtgagc gttacagggc
     2161 gagcagcatc gaagagacgt tcctggctgt gaccggtatg gagaggcgga tcgatgattt
     2221 cgagcggcgc ctggacgctt tttcgcgcta acgcgttgtg tctgatgcgg cggctgcggc
     2281 cggtagcccg gagtagcccc gtactcgcgc tcctcgctcc cgccgggctc atagccggcc
     2341 tggcctgggc cggctggcga ggagcggcga gcatggctgc ttcgaacgac ggcgcttcga
     2401 cggccgtagt cctcacggta ggcatcgttt tcgccatcgt gggcttcaac gtgcagcatg
     2461 tgtcatcggc gggcaggtcg ctggacgccc aggtcagatc cgcgccgctc tctaggttgg
     2521 ggctgttcat gggcaccgta ggcatacctt ttgcaatctg ttgcctcgtc gtctccatat
     2581 tgagcctggc gttgttcgtg ccccttatct acgcggcaga ggctccactt tacgcaccaa
     2641 tcgacctcgc gctcttccaa gccgcggttt tctacgcggc gggagcgtta ggagaggtcc
     2701 tcaagcgcgt gacgcgccgg caacccgcgg cgctgctcgc cgggccgccg ctggtgctgg
     2761 cgtgggtggc aagcggccac gcgacaggtg gcggagcatg gcccggcatc gctcgaccgc
     2821 tcggccacac ggtcctgaac accacaaccg agccggcgct gcggctcacg ggcgcgttgc
     2881 tactcctgct cctcgccgga gtgggtgttt ggatctctgt ggccgctttc ctgggcttgc
     2941 cggagcagca gaccttctcg cacgtaggcc gccagctccg cgctccgggc tccggatccg
     3001 gcgtcgtctc gtcagtggcg ttgaagcgta tggtgcgcga caggtcggtg caaaggcaca
     3061 cgttgtttgt cgccctcgta gcagggacgc tgtcctgcct gacctccgct ttgcttcccg
     3121 acgtcgcgca ggccgcagtc gggggtctcc tcgtgctggc cgcgctgagt gtggcgatcg
     3181 tgcccctggc gacctatgga gcgaaccggg attcgagctg gttctggagg tccacgcccg
     3241 tctccttcgc cgcttacgtt ctcggcatgg tcggcgctgg catttgcgct ggcaccgtgg
     3301 tggtcgtggc gccgacggcc gcggcgctgc ctttcttctg ggtaggacgg gaaccgccgg
     3361 agctcgcggt ggtcgtcgtc gtggtggtgg tcatcttgct cgtggccaca ggagcggggt
     3421 tcctcgtgcc ttgcagcctg gagaacacat ccgagcagat cctctcctac gccgcgttcg
     3481 gcgcgtcgct ggccggcgtt ttcgcggccg ggtcgtgggt tgcgccgcgg ctcacggtcc
     3541 tcgccatccc ggagtcgctc gtcgtggttg gcctcaccct cgtcgtggcc ggcctcgtgg
     3601 tcgccgctgc cttcttgagg gagcgcgagc ggaggaaggt gtgatcttcg atgtcgaggt
     3661 ccctgccttc cctcaggccg ttctactcca gcgtacccat ctcctggata ccgtgactgg
     3721 cgatagggtc tcgctgaacg aaaccgcgta cttcatgctc tcgctggtcg acggcaggcg
     3781 cacagcgaag gacataggcg aagcggtggc ggcagtctac ggtctcgccc cccggcaggt
     3841 aaccagcgac ttcctacaga tcgctgctct actgaacgag aagtgcttgc tgaacttcga
     3901 caccccgcta aggagtcggt gcgccacctt ccccaggacc gtcaggctct tcctcttcga
     3961 cgtcgccctg ggtcagctac ggtttccttg gcgtagaaag cgcctggact tttgcaacga
     4021 aagcagacgg atcggtttgc tatcggtcgt taggcacctg agcctgccgg ctgcgatgat
     4081 gggagcgccg ctctctctag cggtgtggct gctgcttggc gaagttatgt cgtccttctg
     4141 gatcgcggct ggcatcgcgc tcggctttgc ggtgagcctg attctccacg aggcggccca
     4201 cgccgtggcc ctggctccgg tccccgcttt tcttagcctg tacggtccgg tttttctggt
     4261 aggccatccg gagatccctc ccggcaaggg attgctggtc tctgccgcgg gacccgcgat
     4321 agcagggctc acgggcttgc tcctcatgct ggcgagctgt gtcttgcgct cggagcccct
     4381 cgtgttcgcc ggggagatcc tgtcaatgaa cctgctcggc atgacaaccg tcgccgcgga
     4441 cgggcgcaaa gcgctgcgca acctcgcgcg cgtgctcgat cctcccgaga gaaggagcgc
     4501 taaatgttga gggtagcagc ggcgtccttc gtgttcggat tcctcgcggt cggcttcctg
     4561 ctcatctgct ccgctttcgg gctcgccata atcg
//
LOCUS       DASTUV010000294         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_921726, whole genome shotgun sequence.
ACCESSION   DASTUV010000294 DASTUV010000000
VERSION     DASTUV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921726"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFT03_05910"
     CDS             complement(<1..590)
                     /locus_tag="VFT03_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU4847715.1"
                     /translation="MNCPHCQTANPEGARFCLNCGNQLEAPARVDGERKYVTVLFADV
                     VDSTVVAERLDPEQFAEIMNGAFAFLNASVKKFGGTVVRLLGDAVLAFFGAPVAHEDD
                     AERAVRAGLAIQAAAKEYAELIRRSYGVDFDVRVGINTGLAVLATVGDEIRTEYTAMG
                     DTTNVAARMQNAAMPGTVLISADTYHLVKQLFEFKPR"
     gene            complement(701..2389)
                     /locus_tag="VFT03_05915"
     CDS             complement(701..2389)
                     /locus_tag="VFT03_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847716.1"
                     /translation="MVDTSQKAERPVILAVDDEPAVLSAVARDLRREYGEHYRVMRAD
                     SGISALELVRQLKLRNTPVALFLVDQRMPLMSGVDFLAEAKELFPDARRVLLTAYADT
                     QAAISAINEVALNHYLMKPWDPPEEHLYPVLTDLLDDWQASFAPPFEGIRVVGHQLSA
                     QSHQIKDFLSRNLVPYQWLDVEIDEEARDLLEIAGSDGSRLPLVLFPEGPPLTQPSNV
                     EIAEKLGLRTRPGMPFYDLVIVGAGPAGLAAAVYGASEGLSTILVESEAPGGQAGTSS
                     WIENYLGFPGGLSGGDLSRRAVAQASRFGAEILAPLEAKRIRAQDPYRIVDLTSGAEI
                     SCHALLIATGVSYRKLDAPGVERLTGAGVYYGAAITEAMACKGKDVFIVGAGNSAGQA
                     ALYLSRYASSVTLVVRGESLARNMSHYLVSQIESHEKITVLTRTTVAEVKGDARLEEI
                     TLADATTGETQTVPAFALFIFIGARPHTDWVADVVERDPQGFIVTGRSLVRGGRRPKG
                     WHLDRDPFFLETSVAGIFAAGDVRHRSVKRVASAVGEGAMAVQFVHQYLSRVPE"
     gene            complement(2732..3505)
                     /locus_tag="VFT03_05920"
     CDS             complement(2732..3505)
                     /locus_tag="VFT03_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4847717.1"
                     /translation="MGRLTFLGTGDSLNPERAQTSVAVPLAGDETMLIDTSSGTVLLG
                     RLEAAGIPLESVRHVFVSHRHFDHVGGLAPLLTALAALPEASLTVHAAPETLRALRGY
                     LGLTIPGAEGWLGERLGWHELVPGKLIVVGETEITPFLMDHGLECVGFRIARADSVAV
                     FAVDTRPCPEIVEYAGGAGLLLHDTYGPQSASEQAHAMGHSTATDAGEVARAAGVPRL
                     VLTHLRASRLVDPEALVAEAESVFGGPVKAAEDLDIVEF"
     gene            complement(3677..4063)
                     /locus_tag="VFT03_05925"
     CDS             complement(3677..4063)
                     /locus_tag="VFT03_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4847718.1"
                     /translation="MLDLEVVHGYLSRSYWAAGVPEDVVRRSIENSLCFGVYRGEEQA
                     GFARVVTDRATFAYLADVFVLDEHRGQGIGKWLLEVILSHPDLQGLRRWMLATRDAHD
                     LYRRYAFTELARPGIFMERKDASYGG"
     gene            complement(4111..4791)
                     /locus_tag="VFT03_05930"
     CDS             complement(4111..4791)
                     /locus_tag="VFT03_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU4847719.1"
                     /translation="MSDHATIQPPSVSVGDYVKAIWEVAGSGVASTKDVAERLSIAPP
                     SVTNMFVRLREMGYVEYERYRGVSLTERGREEALRLVRRHRLIETFLLEHLGYSWGEL
                     HEEAERLEHAVSDEFTERLSEFLGHPGQDPHGDPIPAADGTMQPDHSFPLSEAAPGQR
                     VCISKVGDKPSTLDYLGEHGLVPGRLLRVREVRDLDGVVIVEDEDGGFHALGEPLARA
                     VFVRAAAG"
BASE COUNT          853 a         1646 c         1505 g          879 t
ORIGIN      
        1 cgcggcttga attcgaagag ctgctttacg agatgatagg tgtcggcgct gatgagcacg
       61 gtaccgggca tggcagcgtt ctgcatccgg gcagcgacgt tcgtcgtgtc gcccatcgcc
      121 gtgtattccg tcctgatctc gtcgccgacg gtggcgagca ccgcgagccc ggtgttgatg
      181 cctacgcgca cgtcgaagtc gaccccatag ctccgcctga tcaattccgc gtattctttt
      241 gccgctgcct ggatagccag acccgctcgt accgcgcgtt ccgcgtcgtc ttcgtgtgcg
      301 accggtgcgc cgaagaacgc gaggaccgca tctccaagca acctcacgac cgtcccgccg
      361 aatttcttta ccgacgcgtt gaggaaggcg aacgcgccgt tcatgatctc cgcgaactgc
      421 tcgggatcta gccgctccgc cactacggtc gagtcgacca cgtccgcgaa gagcaccgtc
      481 acgtatttgc gttcgccgtc cacgcgagcg ggggcttcga gctggttgcc gcagttcaga
      541 cagaacctcg cgccctcggg attggcggtc tgacagtggg ggcagttcat ctcaaggcct
      601 cgctcgaaat aacgtagata ctctcggccc gatcataact acccctacaa ggcaattcaa
      661 ctgctctggc ttcgccgcga gttttggcgt gtaccgtact ctactccggc accctgctga
      721 gatactggtg gacgaactgc acggccatcg cgccctcccc gaccgcggag gcgacgcgct
      781 tcaccgaccg gtggcgcaca tctccggccg cgaagatgcc ggccacgctg gtctccagaa
      841 agaaggggtc ccggtccaga tgccacccct tcggacgccg gcccccgcgc accaggctac
      901 gcccggttac gatgaagccc tgcgggtctc gttccacgac gtcggcgacc cagtcggtat
      961 gcggcctcgc gccgataaag atgaagaggg cgaacgccgg caccgtctga gtctcgccgg
     1021 tcgtggcatc ggcgagggtg atctcctcga gcctggcatc tcccttgact tcggcgacgg
     1081 tagtgcgcgt cagcacggtg atcttctcgt gtgattctat ctgtgacacg aggtaatggg
     1141 acatgttcct tgcgagtgat tcacccctca ctacaagggt caccgagctg gcgtacctgg
     1201 agaggtacag ggcggcctgc ccggcggagt tccccgcgcc gacgataaat acgtccttac
     1261 ctttacaggc catcgcttcg gtgatcgccg caccgtagta cacccctgcg cccgtgagtc
     1321 gttcgacgcc gggcgcgtcg agcttgcggt acgacacgcc agtcgcgatg agcagcgcgt
     1381 ggcagctgat ctcggcaccg ctggtaaggt cgacgatacg ataggggtcc tgcgcccgaa
     1441 tgcgcttcgc ctccagaggc gcgagtatct ccgcgccgaa cctgctggcc tgagccacgg
     1501 ctcgcctcga cagatctcct ccgctcaagc cgcccgggaa ccccaggtag ttctcgatcc
     1561 aggagctcgt accggcctgt ccaccgggcg cctcactttc gacgaggatc gtactgagcc
     1621 cttcggaggc tccgtacacc gccgccgcca gtcccgccgg accagcgcca acgatgacca
     1681 ggtcgtaaaa cggcataccg gggcgcgtcc tcaggccgag cttctccgcg atctctacgt
     1741 tggacggttg cgtcagggga ggcccttcgg ggaagagcac caggggcaac cgcgagccgt
     1801 cggagccggc gatctccagc aggtcgcggg cttcctcgtc gatctcgacg tcgagccact
     1861 gatagggcac gaggttcctc gagaggaagt ctttgatctg gtgtgattgc gccgacaact
     1921 ggtgaccgac cacgcgaatg ccttcgaagg gtggagcgaa ggatgcctgc cagtcgtcta
     1981 gcaggtccgt gagcacggga tagaggtgtt cttcgggcgg gtcccacggc ttcatgaggt
     2041 agtggttgag ggcgacctcg ttgatcgcgc tgatggcggc ctgggtgtcc gcgtacgcgg
     2101 tgagcagaac gcgcctggcg tcggggaaaa gctccttagc ctccgcgagg aaatctacgc
     2161 cggacatgag cggcatccgc tgatctacca ggaacagggc gacaggcgta ttgcgcaatt
     2221 tcaactgccg caccagctcc agcgccgaga tgccggaatc cgccctcatc acccggtagt
     2281 gctcgccgta ttcgcgccgc aggtcgcgcg cgacggcgct caacaccgcc ggctcgtcgt
     2341 cgacggcgag gatgactggc ctctctgcct tttgcgaggt atctaccaca gcttgtccgc
     2401 cacacgctcg atctggatgc gggccgtgcc cggcctgccc cactatagca gggctcgcct
     2461 ccgtgttgcg gctaggttcg cccctctatg acctggccgc atcttccagc gcgtaggcga
     2521 cagcatcatc gagcggcatg gcccgtcctt cggccagcgc cgcctcccac ttcgcctcct
     2581 cgagccggga gcgagcggcg tctcggaatg gttcccgcag agcctcggcc gcgccgaaca
     2641 gcctggcagt ccgttcttct cctcttggtg aacaggaagg ttagggtgtg cggctccgcc
     2701 atccgctttg cgtcctccta taccccgaga atcaaaattc gactatgtcg agatcctccg
     2761 ccgccttcac cggtcctcca aacacggatt cggcctcggc gaccagcgcc tccggatcga
     2821 ccaaccggct tgcgcgcaag tgggtgagga cgagccgcgg cacaccggca gcgcgggcaa
     2881 cttcgccggc atcggtcgcg gtcgaatgtc ccatggcgtg cgcctgttcc gatgcgctct
     2941 gcggaccgta tgtatcgtgg aggagaaggc ctgctccccc agcgtactcg actatctcgg
     3001 ggcaagggcg cgtatcgacg gcgaagaccg caacagagtc cgccctggcg atgcggaagc
     3061 ccacgcattc gaggccgtgg tccatcagga aaggggtgat ctccgtctct cctaccacga
     3121 taagcttgcc gggaacgagc tcgtgccagc caaggcgctc ccccaaccag ccttctgcgc
     3181 cggggatcgt caggccgagg tagcctcgca gcgctcgaag cgtctcgggt gcggcgtgga
     3241 cggtgagaga agcctcggga agggccgcga gggctgtgag caagggagcc agcccgccga
     3301 cgtggtcgaa gtggcggtgg ctgacgaaca cgtggcgcac ggattcgagc gggatgccgg
     3361 cggcctcgag gcgacctagc agtacggtgc cgctggaggt gtcgatcagc atcgtctcat
     3421 cacccgcgag cggcacggcc acactggtct gagcccgctc cgggttcaga gagtctccgg
     3481 tcccgaggaa ggttagacgg cccacctcga tttctaccga tctccccacc gctgcggccg
     3541 gttttctcgt tcgcgaacga tgataacgat gctagtgtac gggttttcga tcatttaccc
     3601 ctcgcatata acacaagacg gtgtcaggcg cgagcatagg catcgaaaga ccgagcgcgc
     3661 ggaggctggg agagcttcaa cctccgtagg aagcgtcttt tctctccatg aagatcccag
     3721 gccgcgcgag ctctgtgaac gcgtacctcc ggtagaggtc gtgggcgtcc ctggtggcga
     3781 gcatccatct tcgcaaccct tgcaggtccg ggtgcgagag gatcacctcg aggagccact
     3841 tcccgatgcc ttgccccctg tgctcatcca gcacgaagac gtcggcgagg taggcgaagg
     3901 tcgcgcggtc cgtcaccacc cgcgcgaagc cggcctgctc ctcgcccctg tacaccccga
     3961 agcacagcga gttctcgatc gaccgcctca ctacatcctc cggtacgccc gcagcccagt
     4021 acgacctgct caagtagccg tggacgacct cgagatccag catcgatgcg tccgtgctga
     4081 tcagataccc gtcccgccgc cacccgcgcg tcatcccgcg gcggcccgga cgaagaccgc
     4141 gcgcgcgagc ggttcgccga gggcgtggaa ccccccgtcc tcgtcctcca cgatgacgac
     4201 gccgtcgagg tcgcgcacct ccctgaccct cagcaggcgg ccggggacga gcccgtgctc
     4261 tccgaggtag tcgagcgtcg agggtttgtc tccgaccttg gagatgcaga cccgctggcc
     4321 cggcgccgcc tcgctcaagg ggaaggagtg gtccggctgc atcgtgccgt ccgcggcggg
     4381 gatggggtcc ccgtgggggt cctggcccgg atgccccagg aactccgaca ggcgctccgt
     4441 gaactcgtct gagaccgcgt gctcgagacg ctcggcctcc tcgtgtagct caccccagga
     4501 gtacccgagg tgctcgagca ggaaggtctc gatcaggcgg tgccgccgta ccagccgtaa
     4561 ggcctcctca cggccacgtt ccgtcagcga aaccccccga tagcgctcgt actcgacata
     4621 gcccatctcc cgtaaacgca cgaacatgtt ggtcacagaa ggcggcgcga tcgacaacct
     4681 ctcggcaacg tctttggtcg aggctacccc agagccggcg acctcccata tcgcctttac
     4741 gtaatcccct accgataccg atgggggctg gatcgtcgcg tgatcgctca tcttctcgcc
     4801 aatcctagcc caccataacc tctatatcaa ggaaggctaa acgctatctt ggagtagcaa
     4861 aatacttgac aacaaccagc tca
//
LOCUS       DASTUV010000295         4611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1675819, whole genome shotgun sequence.
ACCESSION   DASTUV010000295 DASTUV010000000
VERSION     DASTUV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675819"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFT03_05935"
     CDS             complement(<1..543)
                     /locus_tag="VFT03_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847720.1"
                     /translation="MPSKVAYATTVLLTAGVVAAELLHAPILLIFAVSALALVGLAWV
                     LGQATESLGHHAGPRVGGILNATFGNAAELIITIFALAAGLTTVVKASIIGSIIGNVL
                     LVLGASLLLGGLKNGAQTFSATIAGINASMLAIVAAALSLPTIFAATNPGQGPATLSV
                     EVAVVMFVLYVLYLLFYLRSP"
     gene            648..1406
                     /locus_tag="VFT03_05940"
     CDS             648..1406
                     /locus_tag="VFT03_05940"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEU4847721.1"
                     /translation="MIDYHLHVVAHAERPMTVENILAYCEVARERGILQMGITEHDRY
                     LDEIDLAAFQEAREKSRDVELRLGIEIDFVPGDEVRMEHDSNALPYDYVIGSVHRVDG
                     EEVDHPDHREIYEKWDTYDLYEAYYANVRAVALSGGFDVLGHPDLIKIFRQYPDGDIT
                     GMLEETADAVAESGIVVDVNAAGLRKPVGEIYPSRKLLEMFYRRGVPIILSSDAHAPD
                     QVGMGYEKSLPLVRDVGYREVVTFSARERGTLPL"
     gene            1403..2113
                     /locus_tag="VFT03_05945"
     CDS             1403..2113
                     /locus_tag="VFT03_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEU4847722.1"
                     /translation="MTEELFLGDSYLREFEAVVVELSGREVILDRTAFYPGGGGQPPD
                     RGTLGIGPVHASVVDARREGRQVVHVLDNPIPDTVRELEGSLDWERRYGHMRHHTALH
                     VLSGVIWRNFGAKVTGGQMRSDRARMDFSFPEEWTTGVVGEIERLTNEALAEDRPVKV
                     YELPREEALENPDLIRTQVNLVPERVRMIRIVEIEGIDTQADGGTHVANTGEVGEIEI
                     TGHKSKGRQNKRVEFVLR"
     gene            2110..2598
                     /locus_tag="VFT03_05950"
     CDS             2110..2598
                     /locus_tag="VFT03_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU4847723.1"
                     /translation="MRASIIALFLLALLLAGCGSSGAGASASPGAETTGTATELRTVT
                     IDTSGGGKVELRVEIADDTPEQTRGLMGRTALGDDRGMLFVYPDEEVLSFWMKNTLIP
                     LSIAFIASDGTIVDLQDMKPLDDDPPHYVSAEPARYALEVNKGYFEKHGIKVGDRADL
                     SV"
     gene            complement(2595..3710)
                     /locus_tag="VFT03_05955"
     CDS             complement(2595..3710)
                     /locus_tag="VFT03_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847724.1"
                     /translation="MSGTSERRITDLPTLIEALEPEDRAAAGRLFDVVATTGRLDPPE
                     AMRAWIERSFGSLEVVTEQRVIKVTNRATLEGSLFNGLRASRPLDTSVSVDLEREIEA
                     TVGDPFCRPEECTPADVFGRVRGEYSVTASNVAKYDGFHGVVVFDDHNPLHLTPEKVS
                     DYISVALEWCRRALRTDPEAKYPFLMWNCLWRAGGSIIHGHAQVTVTRGAHYPKIEHL
                     RRAAVSYGARHGFDYFEDLCRVHGALGLGIPAEGGARAFASLVPIKEKELVVIGRTPE
                     DEGLQRTVGALLQGYLQTLGVRAFNVAFYMPPLTPSEEDWSGFPTVVHMVDRGSPANR
                     TSDIGAMELYAASVVASDPFRVADLLRNGPLEDTSHG"
     gene            complement(3736..4215)
                     /locus_tag="VFT03_05960"
     CDS             complement(3736..4215)
                     /locus_tag="VFT03_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4847725.1"
                     /translation="MGESVETLRAGSAEEDLRLRLVDEFAGFGPSYMKWVKSRLRDRG
                     LTYARIRLIGALHCGGPQIMSSISEELGVTRRNVTALVDALEEEGLVRRQPHPTDRRA
                     TVIELTEEGERTAGARYDEHREAVAELFGGLSEEDQRDLIRVLGRLRDALRREGFSC"
     gene            4326..>4611
                     /locus_tag="VFT03_05965"
     CDS             4326..>4611
                     /locus_tag="VFT03_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847726.1"
                     /translation="MKRLIVGEVEITALTDIEGPFFRLSQLLPGVGADLWEPYVGRYP
                     WAFADAGTLYGRVGAYVLRSPGSVVLVDTGIGRGAMGRRGRLIEDLERNGA"
BASE COUNT          855 a         1467 c         1509 g          780 t
ORIGIN      
        1 ggggctgcgg aggtagaaga gcaggtagag gacgtagagg acgaacatca ccaccgcaac
       61 ctcgacgctc agggtggcgg gcccctgacc cgggttcgtg gcggcgaaga tcgtgggtag
      121 tgagagcgcg gcggcgacga tagcgagcat cgaggcgttt atcccggcaa tcgtcgccga
      181 gaaggtctga gcgccgttct tgagcccgcc gagaagcaag ctagccccta gcacgagcag
      241 gacgttgccg atgatcgagc cgatgatcga agccttcacg accgtggtca ggcccgcagc
      301 cagcgcgaag atggtgatga tgagctctgc ggcgttcccg aaggtggcgt tgagtatgcc
      361 cccaacccgt ggcccggcgt gatggcctaa agactccgtg gcctgtccca acacccacgc
      421 gagcccgacg agggcgaggg cggagacggc gaagataagc aggatgggcg cgtgcagtag
      481 ctcggcggcc accacgcccg ctgtgagcag gacggtagtc gcgtaggcta ccttactcgg
      541 caacgcgctt ctctcgttag agcaaacaca attaccatct tacccagata aacgcggcgg
      601 ctatcctata agataaacag gttggacgtt gcttgttaag gagacctttg atcgactacc
      661 accttcacgt cgtcgcccac gcagagcggc cgatgacggt cgagaacata ctcgcctact
      721 gcgaggtcgc aagagagcgc ggcatcctcc agatgggcat caccgagcac gaccgctacc
      781 tcgacgagat agatctggcc gccttccagg aggcgcggga gaagtctaga gacgtggagc
      841 tgcgcctggg gatagaaata gacttcgtcc ccggcgatga ggtgcggatg gagcacgact
      901 ctaacgccct cccttacgac tacgtcatcg ggagcgtgca cagggttgac ggggaggagg
      961 tggatcaccc cgaccacaga gagatctacg agaagtggga cacctacgac ctctacgaag
     1021 cctattacgc gaacgtgcgg gcggtcgctc tctccggagg cttcgacgtc ctcggccacc
     1081 ccgacctcat aaagatcttc cgccagtacc cggacggaga catcaccggc atgctcgagg
     1141 agaccgcaga cgccgtggcg gagtcgggca tcgtcgttga cgtgaacgcc gcggggttgc
     1201 gcaagccggt cggtgagatc tacccctcca gaaagcttct cgagatgttc tatcgccgcg
     1261 gcgtgcccat catcctctcc tccgacgccc acgcccccga ccaggtcggg atgggctacg
     1321 agaagagcct cccgctcgtc cgcgacgtcg gctaccgcga ggtcgtgacc ttctccgccc
     1381 gcgagcgcgg aaccctgccg ctgtgaccga agagctcttc ctcggggact cgtacctgcg
     1441 cgagttcgag gccgtggtcg tcgagctctc ggggcgggag gtgatcctgg accgcaccgc
     1501 cttctacccc ggaggcgggg gccagccgcc ggacagggga actctgggta tcggccctgt
     1561 ccacgctagc gtggtggacg cccggcgcga aggccgccag gtcgtccacg tcctcgataa
     1621 ccccatcccc gacacggtgc gggagctcga ggggagcctt gactgggaac ggcgttacgg
     1681 ccacatgcgc caccacaccg ccctgcacgt cctctccggc gtcatctggc ggaacttcgg
     1741 ggcgaaggta acaggaggcc agatgcgctc ggaccgcgcc aggatggact tctccttccc
     1801 agaggagtgg accacgggcg tcgtcgggga gatcgagcgg ctcacgaacg aggctctcgc
     1861 cgaagatcgg cccgtgaagg tttacgagtt gccgcgcgaa gaagccctcg agaaccctga
     1921 cctaatccgc acccaggtca acctggtgcc cgagcgggtg cgcatgatcc ggatcgtcga
     1981 gatcgagggt atagacaccc aggccgacgg cggaacccac gtcgccaaca ccggcgaggt
     2041 gggagagata gagataaccg gccacaagag caagggccgc cagaacaagc gcgtggagtt
     2101 cgtcctacga tgagagcgtc cataatagcc ctcttcctgc tcgcgctgct cctcgccggg
     2161 tgcggatcgt ccggcgcagg ggcgagcgcc tccccgggag cagaaacgac cggcaccgct
     2221 accgagctgc gcaccgtgac catcgacacc tccggcggcg ggaaggtcga gctgagggtg
     2281 gagatcgccg acgatacccc cgagcagacc aggggactca tggggcgtac cgccctcggc
     2341 gatgacaggg gtatgctctt cgtctacccg gacgaggagg tactttcctt ctggatgaag
     2401 aacaccctca tccccctctc catcgccttc atagcctccg atggcaccat cgtggacctc
     2461 caggacatga aacccctgga cgacgacccg ccacactacg tctccgccga gcccgcccgg
     2521 tatgccctcg aggtcaacaa gggctacttc gagaagcacg gcataaaggt gggggacagg
     2581 gcggacctgt cggtttagcc gtgactagtg tcttcgagcg gaccgttgcg tagcaggtcc
     2641 gctaccctga aagggtccga ggcgaccacc gaggccgcgt agagctccat cgcaccgatg
     2701 tcggaggtgc gattggccgg gctcccacgg tccaccatgt ggaccacagt ggggaacccg
     2761 gaccagtctt cctctgacgg agtgagcggg ggcatgtaga acgcgacgtt gaaggcccgg
     2821 accccaaggg tctggaggta gccctggagc agagccccga ccgttcgctg aagaccctcg
     2881 tcctccgggg tccggcctat cacgactagc tccttctcct tgataggaac gaggctcgcg
     2941 aaggctctgg cgcccccttc ggcagggatg cccaggccca gagcgccgtg gacacggcac
     3001 aggtcttcga agtagtcgaa gccatgtctg gctccgtaag acactgcggc ccgccggagg
     3061 tgctcgatct tcgggtagtg ggcgccgcgg gtaactgtta cctgggcgtg gccgtggatg
     3121 atcgagccgc ctgcgcgcca gaggcagttc cacatgagga aggggtactt tgcctcgggg
     3181 tcggtccgga gagccctgcg gcaccactcg agggccacgg aaatgtagtc ggagaccttc
     3241 tcgggggtga ggtggagggg gttgtgatcg tcgaagacga cgacgccgtg gaagccgtcg
     3301 tatttggcga cgttcgaggc ggtcaccgag tactcacccc tcaccctccc gaagacgtcg
     3361 gcgggggtgc actcctccgg gcggcagaac gggtccccga ccgtggcttc tatctcgcgc
     3421 tcgaggtcca cgctcacgct ggtatccagc ggacgtgagg cgcgcagccc gttaaagagg
     3481 cttccctcca gggtcgcgcg gttcgttacc ttgattacgc gttgctctgt caccacctca
     3541 agagagccga aggacctttc gatccaggcg cgcatcgcct cgggcgggtc gaggcggccc
     3601 gttgtggcga ccacgtcgaa gaggcgcccg gcggcggcgc ggtcttcggg ttcgagagcc
     3661 tcgatgagag tggggaggtc ggtgatcctg cgttccgacg ttccgctcat gacctgcgcc
     3721 cggcctggat cgccgctagc aggaaaagcc ctcgcgccgc agcgcgtcgc gcaaccgccc
     3781 gagcacccgg atcaggtctc tctggtcctc ctccgagagc cccccgaaca gctcggcgac
     3841 cgcctccctg tgctcgtcgt acctcgcgcc agcggtccgc tctccctcct cggtcaactc
     3901 gatgacggtg gcccgccggt ccgtcgggtg tggctgccgc cggactagcc cctcctcctc
     3961 cagagcgtcc accagcgccg tgacgttgcg ccgcgtgacg ccgagctcct cgctgatgct
     4021 gctcatgatc tgggggcccc cgcaatgtaa cgctccaatt aacctgatcc gcgcgtaggt
     4081 cagacccctg tcccgtaacc gcgactttac ccacttcatg taggaaggtc cgaagccggc
     4141 gaactcgtca acgagccgca gccgtagatc ctcctcagcc gacccagccc tcagcgtctc
     4201 cacactctca cccacgatgt ccatgttggt aactcgactt gcccagttta ctgttgacgt
     4261 tcattgttgt caatgttatt gtgaacatgt acattatagc cgggtgcagg gatagtaatg
     4321 gcgtcatgaa gaggctcata gttggagagg tcgagatcac ggcgctgacc gacatcgagg
     4381 ggccattttt caggttgagc cagctcttac ctggagtggg ggcggacctg tgggagcctt
     4441 acgtcggacg ctatccgtgg gcgttcgctg acgcgggcac gctctatggg cgcgtcggtg
     4501 cttatgtgtt gagatcgccg gggagtgtgg tgctcgtgga caccgggatc gggcgtggcg
     4561 ccatggggag gcggggcagg ttgatagagg acctcgagag gaacggcgcc t
//
LOCUS       DASTUV010000296         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_380724, whole genome shotgun sequence.
ACCESSION   DASTUV010000296 DASTUV010000000
VERSION     DASTUV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380724"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="thrS"
                     /locus_tag="VFT03_05970"
     CDS             <1..975
                     /gene="thrS"
                     /locus_tag="VFT03_05970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU4847727.1"
                     /translation="RLKPMNCPSHMTLYNSEAHSYRDLPLRYAEFATLYRYEKSGELT
                     GLTRVRALTQDDAHIFCTEEQIQEEFRRALEIIREVLDTYGFTDYRVRLSLRDPDDPK
                     YLAGDERWSRAEDALRTALDASGIDYYTGIGEANFYGPKADFIAKDVLGREWQLSTIQ
                     VDFFQPGRLGCEYVGEDGNTHTPVLLHRAVTGTTERFMAVVIEHYGGAFPVWLSPVQA
                     VVIPVADRHLDYAREVQGRLVEKGLRIEVDDSANSMQKKIRENARQKIPYLLIVGDRE
                     AEQGAVNVRRRAEKQQEALDVEEFAERVLREVASREENPVDTGGGRGV"
     gene            1102..1659
                     /gene="infC"
                     /locus_tag="VFT03_05975"
                     /pseudo
     CDS             1102..1659
                     /gene="infC"
                     /locus_tag="VFT03_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            1762..1959
                     /gene="rpmI"
                     /locus_tag="VFT03_05980"
     CDS             1762..1959
                     /gene="rpmI"
                     /locus_tag="VFT03_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU4847728.1"
                     /translation="MPKMKTHQGAAGRFEVRKNGKLRRRRSGHNHMLEKKSSKRKRRL
                     NTETYINPKDEKRIKRLLGVR"
     gene            1960..>2637
                     /gene="rplT"
                     /locus_tag="VFT03_05985"
     CDS             1960..>2637
                     /gene="rplT"
                     /locus_tag="VFT03_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU4847729.1"
                     /translation="MARTARSLHARKKRRKVLEQAKGYRGTKHTSYKRAKEQVWKSGV
                     YAYEGRKQRKRDFRSLWIQRINAGAREHGLSYSQFVHGLRVAEIDLDRKILADLAATE
                     PEAFAAIVAQAKNALDGKPVERRIEVDRRRAEEAPQARQKPKRSKPEPETTAPEPQED
                     TEVEASGAAERGAEGRVVVGDVEEAAEEEGKVNATEAAGRKAEELGVDLRSVEGTGKD
                     GRITVGDV"
BASE COUNT          578 a          788 c          902 g          369 t
ORIGIN      
        1 cgcctcaagc cgatgaactg cccgagccac atgacgctct acaactcgga ggcgcattcg
       61 taccgtgacc tgcccttgcg ctacgccgag ttcgccacgc tctaccgcta cgagaagagc
      121 ggcgagctta cgggcctcac ccgggtgcgg gccctcactc aggacgacgc ccacatcttc
      181 tgcaccgagg agcagatcca ggaggagttc aggcgggcgc tggagatcat ccgcgaagtc
      241 ctcgacacct acgggttcac ggattatagg gttcgtctca gcctgcgcga cccggacgat
      301 cccaagtacc tcgcgggcga cgagcgctgg tcgcgggccg aggacgcttt acgcaccgcg
      361 ctcgacgcgt ccggcatcga ttactacacc ggcatcggag aggccaactt ctacggcccc
      421 aaggccgact ttatcgccaa ggacgtgctc ggacgggagt ggcagctctc caccatacag
      481 gtggacttct tccagccggg caggctcggg tgcgagtacg tgggcgagga cggcaatacg
      541 cacacgccgg tcttgctgca ccgcgccgtt acagggacga cagagcgttt catggcggtc
      601 gtgatagagc actacggcgg cgccttccct gtgtggctct cgccggtgca ggcggtggtc
      661 atccccgtcg ccgaccggca cctcgactac gctcgcgagg tgcagggaag gctcgtcgag
      721 aagggcttgc gcatcgaggt tgacgactcg gcgaacagca tgcagaagaa gatccgggag
      781 aacgctcgcc agaagatccc ttacctgctc atagtcggcg acagagaggc ggagcagggt
      841 gcggtcaacg tgcgccgccg cgcagagaag cagcaggagg cactcgatgt cgaggaattc
      901 gccgagcgag tgttgcggga ggtcgcctcg cgcgaggaaa accctgtgga cacgggcggc
      961 gggcgaggag tataattgtt ggtgtagtgc tggggcccgc gtccgttcga cgctttagcg
     1021 aaaccaatcg ttgggcgggc tattggcccc gaaattcgtt tttgagagga gtggtgcaca
     1081 gtagcaactg aagaagagcc gaggatcaac ggtcagatac gggcgcgttc cgtgcggttg
     1141 atctcggctg acggggagca attggggata aagcacatcc gcgaggcgac ggactacgcc
     1201 gagcggcagg atctcgacct cgtcgaggtc gccccgaatg cgaatccgcc ggtggtccgc
     1261 ctgatggatt acggcaagta caaataccag aaggagcagg cccgcaaggc cgcccgcaag
     1321 aagcaggtca acatcaacgt gcgggagatc aagctccgtc cgaagatagg cgaccacgat
     1381 ttcaacacca agcggggcca cgtcgagcgt ttcttgcgcg gtggcgacaa ggtgaaagtt
     1441 acgatcatgt tcaggggccg tgaggtgcag catccggagc tcggcgagag gctactcagg
     1501 aggctcgcaa gcgacctcga ggaccttggt cgcatcgaga gccagccgaa cctcgacggc
     1561 cgcaacatgg tcatggtcat ggctccgaga aaggaaaaca aggaggcgca ggcagcccag
     1621 cagaccaacg ggcgtcgcga gagggctgcc cgcaactaaa gcaacagttt gatacaatag
     1681 cgcctcgtcg ggtgcgaaac ggctctctcc cgtgccgcga gccccgtttg taaagctgag
     1741 aacgaaacgc ggaggagatc catgccgaag atgaagaccc accagggcgc cgccgggcgc
     1801 ttcgaggtga ggaagaacgg caagctcagg cgccggaggt ccggccacaa ccacatgctg
     1861 gagaagaaga gcagcaagcg caagcggcgt ctgaacaccg agacttacat caaccccaag
     1921 gacgaaaagc gcatcaagcg ccttctgggg gtgaggtagt tggcacgcac ggcacgcagc
     1981 ctccacgccc gcaagaagcg ccgcaaggtg cttgagcagg ccaaaggcta cagggggacg
     2041 aagcacacct cgtacaagcg ggccaaggaa caggtctgga agagcggcgt ctacgcttac
     2101 gagggccgca agcagcgcaa gcgcgacttc aggtcgctct ggatccagcg catcaacgcc
     2161 ggggcgcgcg agcacggtct ctcttactcg cagttcgtcc acggcctgag agtggccgag
     2221 atagacctcg accgtaagat cctggccgac ctcgccgcga ccgagcccga ggccttcgcc
     2281 gctatcgtgg cccaggcgaa gaacgccctc gacggcaagc cagtagagcg gcgcatcgag
     2341 gtggaccgcc ggagggccga ggaagcgccg caggcgcggc agaagcccaa gaggtcgaag
     2401 cccgaacccg agacaaccgc tccggagcca caggaggaca ccgaggtcga ggctagcggc
     2461 gcagccgagc gcggggcgga gggtcgcgtc gtcgttggcg atgttgagga ggccgccgag
     2521 gaggagggca aggttaacgc cacagaggca gccgggcgca aggcggagga gctcggtgtg
     2581 gatctcagaa gcgtcgaggg cacgggcaag gacggccgca tcaccgtcgg cgacgta
//
LOCUS       DASTUV010000297         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_536760, whole genome shotgun sequence.
ACCESSION   DASTUV010000297 DASTUV010000000
VERSION     DASTUV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536760"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1366)
                     /locus_tag="VFT03_05990"
     CDS             complement(2..1366)
                     /locus_tag="VFT03_05990"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU4847730.1"
                     /translation="MESTQVQGRLDLETLKAEVDAGRIDTVLIAMTDMQGRLQGKRLT
                     ASHFLEEVVEHDAEGCNYLLAVDVDMNTVEGYAMSSWERGYGDFVFKPDLETLRIVPW
                     QEGTAHVACDLMWEDGSAVVASPRQILKRQLERLSERGLEAFVGTELEFLVFMDTYEE
                     AWRKGYRNLEPANYYNVDYSLLGTARIEPLLRRIRNSMAAAGLAVENAKGECNFGQHE
                     INFKFDRALATADGHAIYKNGAKEIAAQEGYSISFMPKFDEREGNSCHIHLSLRGEDG
                     TPVFADGHGFSKTFESFIAGQLACLREFTLFFAPNINSYKRFAVGSFAPTAVAWGKDN
                     RTCSMRVVGHGSSLRVENRLPGGDVNPYLAIAAMIAAGLHGMDNELPLEDEMAGNAYE
                     ADKPHVPTNLREARELFANSEAARDAFGEEVVEHYLNMARVEIEAFEAAVTDWERYRS
                     FERL"
     gene            complement(1382..2845)
                     /gene="eat"
                     /locus_tag="VFT03_05995"
     CDS             complement(1382..2845)
                     /gene="eat"
                     /locus_tag="VFT03_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HEU4847731.1"
                     /translation="MSEQQERRTRQGAGYENVGQDYMEQRQLRKGAAGWVLLAGLGIA
                     YVISGDAAGWHFGLGVGGWGGLLIATLLMATMYTAMVFALAELSAAIPTAGGGYAFAR
                     RAMGPWGGFLTGTAILIEYTLAPAVVATFIGGYMDTLIGFGGPIVYLLCYLVFVGIHL
                     YGVGEALKVMFAITIVAVVAIAAFVIGMIPQFEVANLFDIDPTTAAGASPFLPFGMVG
                     IWAAFPFAIWFFLAIEGVPLAAEETRDPARDMPKGLIAGMAALLLFAILILVFGPGGA
                     GSAVFTSDEMLAAPSVLPLALEEAYGGSNALSTFVNIAGLAALIASFFSIIFAYSRQL
                     FALSRAGYLPRLLSVTGGRRTPWVALVVPAVIGFLLSLTGAGATLLNSAVFGAAISYV
                     LMTLSHIVLRQREPDLDRPYRTPGGVITSGVACVLAVLAVIATFIVDVRAAFIMLGVY
                     ALFIAYFALYSRHHLVAQAPEEEFATIERAESELAGS"
     gene            3152..>3400
                     /locus_tag="VFT03_06000"
     CDS             3152..>3400
                     /locus_tag="VFT03_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4847732.1"
                     /translation="MSGQPLYLQVERRIEDLLLQGRYKAGDRIPPEADLVGSLGVSRV
                     TVRAGLTRLVERGVLERRQGSGTFLVRPPEGARLQSGLE"
BASE COUNT          608 a         1108 c         1106 g          578 t
ORIGIN      
        1 ctcataggcg ctcgaaggat cggtagcgct cccagtctgt gacggcggcc tcgaaggcct
       61 cgatctcgac ccgcgccatg ttcaggtagt gctcgacgac ctcctcgccg aacgcatctc
      121 tggccgcctc gctgttcgcg aagagctccc gtgcttctct gaggttcgtc ggcacgtgcg
      181 gcttgtccgc ctcgtaggcg ttgccggcca tctcgtcttc caggggcagc tcgttgtcca
      241 tgccgtgcag gccagcggcg atcatggcgg cgatggcgag gtacgggttc acgtcccctc
      301 cgggcagccg gttctccacg cgcaaggaac tcccgtggcc gacgacgcgc atggagcagg
      361 tacggttgtc cttgccccag gctacggctg ttggggcgaa ggagccgacg gcgaaccgct
      421 tgtaggagtt tatgttcggg gcgaagaaca gcgtgaactc gcgcaggcac gcgagctgac
      481 ccgctatgaa gctctcgaac gtcttggaga agccgtgacc atcggcaaat acgggcgtgc
      541 cgtcctcgcc gcgcaggctc aggtggatgt ggcaggagtt cccctcgcgc tcgtcgaact
      601 tgggcataaa ggagatggag tatccttcct gagccgctat ctccttcgcg ccgttcttgt
      661 agatcgcgtg cccgtctgcg gtggctaggg cgcggtcgaa cttgaagttg atctcgtgct
      721 gcccgaagtt gcactcgccc ttggcgttct ccacagcgag cccggccgca gccatggagt
      781 tcctgatgcg cctgagcaga ggctcgatcc gcgccgtccc gagcagcgag tagtccacgt
      841 tgtagtagtt ggccggctcg aggttccggt accccttgcg ccacgcttcc tcataggtgt
      901 ccatgaagac gaggaactcg agctcggtcc cgacgaaagc ttccagcccg cgctcggaga
      961 ggcgctcgag ctggcgcttg aggatctggc gcggcgacgc caccaccgcg gagccgtcct
     1021 cccacatcag gtcacacgca acgtgcgccg tcccctcctg ccagggcacg atccggagcg
     1081 tttcgaggtc gggcttgaag acgaagtcgc cgtaaccccg ctcccaggag gacatcgcgt
     1141 agccctccac ggtgttcatg tccacgtcca cggcgagcag gtagttgcac ccctcggcgt
     1201 cgtgctcgac gacctcctcg aggaagtggg aggcggtgag ccgcttgccc tggaggcggc
     1261 cctgcatgtc cgtcatcgct atgaggacgg tgtctatcct tccggcgtcg acctccgctt
     1321 tgagcgtttc gaggtcgagg cggccctgga cctgcgtaga ctccactgtt tttactcctc
     1381 tctagcttcc ggccagctcg gattcggccc gctctatcgt agcgaactcc tcctccggtg
     1441 cctgcgccac gaggtggtgg cggctgtaga gggcgaagta cgcgataaag agcgcgtaga
     1501 cgccgagcat gatgaacgcc gccctgacgt ccacaataaa cgtcgcgatg accgcaagaa
     1561 ccgccagcac gcacgctacg cccgaggtta tgacgccgcc cggcgtgcgg taggggcgat
     1621 ccagatccgg ctcgcgctgg cgcagcacga tgtgcgagag cgtcatcagg acgtaggaga
     1681 tcgccgcgcc gaagactgcg ctgttgagca gggtcgctcc ggcgccggtg agcgagagga
     1741 ggaagccgat gacggcaggg accacgagcg cgacccacgg cgtcctccgg ccgccggtga
     1801 ccgagagtaa cctcggcagg tagccggccc tggacagagc aaagagctgg cgcgagtagg
     1861 cgaagatgat cgagaagaag ctcgcgatca gcgctgcgag ccccgcgatg ttgacgaagg
     1921 tgctgagcgc gttggagccg ccgtaggcct cctccagcgc caggggcagc acagaggggg
     1981 ccgccagcat ttcgtcgctt gtgaacactg ccgaccctgc ccccccaggg ccgaaaacga
     2041 ggatgaggat cgcgaacagg agcagcgccg ccatgccggc gatgagccct ttgggcatgt
     2101 ccctcgctgg atctcgggtc tcctcggcgg cgagtggtac gccctctatg gcgaggaaaa
     2161 accagatcgc gaacgggaac gccgcccaga tgcccaccat gccgaacggc aggaacgggc
     2221 tcgcgccggc cgcggtcgtc gggtctatgt cgaagaggtt ggccacctcg aactgcggga
     2281 tcatgccgat cacgaacgcc gcgatcgcca cgacggccac gatcgtgatc gcgaacatca
     2341 ccttgagcgc ctccccgaca ccgtagaggt ggatgccaac aaagactaag tagcacaaga
     2401 ggtacacgat aggcccacca aatccgataa gcgtatccat gtaccccccg ataaaggtgg
     2461 cgacgaccgc cggggcgagg gtgtactcga tcaggatcgc cgtccccgtg aggaacccgc
     2521 cccacggccc catcgcccgc cgggcgaacg cgtagccccc gcccgcagtt ggaatcgcgg
     2581 ccgaaagctc ggctaaggcg aacaccatcg cggtgtacat cgtggccatg agcagggtcg
     2641 cgatgagcag tccgccccac ccgcccacgc cgagaccgaa atgccagccc gcggcgtccc
     2701 ccgagatgac gtaggctatc ccgagcccag ccagcagcac ccagccagcc gcgcccttcc
     2761 ggagctggcg ttgctccatg tagtcctggc ccacgttctc gtacccggcc ccttggcggg
     2821 tccttctctc ctgttgttcg ctcacctcga actcctttcc ccaaagatct gccggaatcg
     2881 cctacgacaa accaccgcag agacagcgcc tctggcccga taacctggcc acccgcacgg
     2941 tctcgcgcaa actaccaacc catcaccctt tacccgtcgg cccgcttgcc gcgatcgcat
     3001 cataattaat accaatttgt tatacaaggg gttggtatgg tctaccctga cggtggtcgg
     3061 gtaggattca gtgtttgtgg gccgggcggt gaggcggttt cggcgaagaa aggggtcggg
     3121 gatcaggctg gatcgccaca actcgaggtc ggtgagcgga cagccgctct acctgcaggt
     3181 agagcggcgc atagaggacc ttttgctgca gggccgctac aaagccgggg accggatacc
     3241 acccgaggcc gatctggtag gctcgttggg cgtgtcgcgg gtcacggtaa gggcggggtt
     3301 gacgcggctc gttgagcgtg gggtgctgga gcgccgccaa gggagcggga cgttcctggt
     3361 gcggccgccc gagggggctc ggctgcagtc aggactcgag
//
LOCUS       DASTUV010000298         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_905181, whole genome shotgun sequence.
ACCESSION   DASTUV010000298 DASTUV010000000
VERSION     DASTUV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905181"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /locus_tag="VFT03_06005"
     CDS             <1..1912
                     /locus_tag="VFT03_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847733.1"
                     /translation="ENGRVKAVETSRGRIETEYVLSCAGMWGPRVGRMAGAFVPLLTP
                     MQHQYAWTTPVPGLEPVSEESDHVILRHQDHAMYFRQVGERYGIGSYKHRSMPVSPDE
                     IPRKYEPGREMPSIMEFTPEDFEGPWEESRKLLPALQKTKIERGINGLFSFTPDGGSL
                     LGESREVRGFWMAEAIWVTHAAGAARMIAEWMTDGAPSIDPSGLDVHRFDDFQKSPSY
                     VLTRSSQSFQEVYDIIHPQQPMEEPRPLRTSPFYPRQRELGAYFLEASGWERPQWYGA
                     NDSMLAEYDVPEMEEWAGRYWSPIVGAEHQAARERGALIDMASLKKAEVSGPGALGFL
                     QKLNTNQLGKPIGSVTYTLMLDQKAGIRSDITVARLGEEHFQLGLNGPRDIEWMERHL
                     PDDGLVQIRDISGGTCCVGVWGPQARELVQSLSPDDLSNEAFGFFKARHIYVGEVPVV
                     ALRVSYVGELGWELYASADLGLRLWDVLYRAGRPLGMIAAGRGAFSGMRLEKGYRLWG
                     TDMTTEHDPYEAGLGFAVKLDKGEFVGREALLRRKEEGPRRRLSCLLLDDPSVVVMGS
                     EPVYAEGRPVGYVTSAAYGYSIVQSIAYAWLPPGLSEEGAKVEIEYFGKRHGATVAEE
                     PLLDPAMKRMRG"
     gene            1928..>2662
                     /locus_tag="VFT03_06010"
     CDS             1928..>2662
                     /locus_tag="VFT03_06010"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEU4847734.1"
                     /translation="MSTKYASGHRVAVSSVLDGIGDTPLVPVEGIYCKLEYLNPSGSI
                     KARIAKYMVERAEREGLLKPGDTIVEASSGNTGNALSMVAAVKGYRMLVVMPEGMSSE
                     RAVISRAFGAEVLEVGHFHVNEALAKAKKLGRQDGYFCPSQFESEWNVEENRVWLGPE
                     ILSQLPEGTVPDALVMGVGTGGTLVGVGQAFRAVNPDVKLFAVEPDESCTILCGETGK
                     HAIEGISDGFVPGIFERHGGLVDGVLS"
BASE COUNT          499 a          796 c          925 g          442 t
ORIGIN      
        1 cgagaacgga cgcgtcaagg cggtcgagac ctcgcggggc cgcatcgaga cagagtatgt
       61 gttgagctgc gccgggatgt gggggcctcg agtcgggagg atggcggggg ccttcgtgcc
      121 gctcctgacg ccgatgcagc accagtacgc ctggaccacg ccggtgccgg ggcttgaacc
      181 ggtctccgag gagtcagacc acgtcatcct taggcaccag gaccacgcga tgtacttccg
      241 gcaggtcggc gagcgttacg ggatcggctc ctacaagcac cgctcgatgc cagtctcgcc
      301 ggacgagatc ccgaggaagt acgagcccgg gcgggagatg ccctcgatca tggagttcac
      361 cccggaggac ttcgaggggc catgggaaga gtcgcgcaag ctgcttcctg cgctgcagaa
      421 gaccaagata gagcgaggca taaacgggtt gttctctttc acgccggatg gtgggtcgct
      481 gctcggcgag tccagggagg tacgcggctt ctggatggcg gaggccatct gggtgacgca
      541 cgcggcgggc gcggccagga tgatagccga gtggatgaca gacggcgccc cgagcattga
      601 cccgagcggg ctggacgttc accgcttcga cgacttccag aagagcccct cctacgtgct
      661 gacccgcagc tcgcagtcct tccaggaagt ctacgacatc atccaccccc agcaacctat
      721 ggaggagccg cgaccgttgc ggaccagccc cttctaccca cgtcagaggg agctcggggc
      781 gtacttcctg gaagcctccg gctgggagcg cccgcagtgg tacggggcca atgactcgat
      841 gctcgccgaa tacgacgtcc ccgagatgga ggagtgggca gggcggtact ggtcgcccat
      901 cgtcggagct gagcaccagg cggctcgcga gagaggcgct ctcatcgata tggcttcact
      961 aaagaaggcc gaggtcagcg gccctggggc gctcggcttc ctgcagaagc tcaacacgaa
     1021 ccagctcggc aaacccatag gcagcgtcac ctacactctg atgttggacc aaaaggcggg
     1081 tatcaggagc gacattactg tcgcgcggct cggtgaggag cacttccagc tcgggctcaa
     1141 cggaccgcgg gacatcgagt ggatggagcg tcacctgccc gatgacggct tggtgcagat
     1201 tcgggacatc tccggcggca cctgctgcgt tggggtctgg gggccgcagg cccgcgagct
     1261 tgtgcagagc ctgagtcccg acgacctctc caacgaggcg ttcgggttct ttaaggcgag
     1321 gcacatctac gtcggcgagg tgcccgtcgt ggcgctcagg gtctcctacg tcggcgagct
     1381 cggctgggag ctctatgcct ccgctgacct gggcttgcgg ctctgggacg tgctctaccg
     1441 ggcgggacgg ccgctcggca tgatcgccgc aggacgcggg gccttcagcg gcatgaggct
     1501 ggagaaaggt taccggctgt gggggacaga catgaccacc gaacatgacc cctacgaagc
     1561 agggctcggt ttcgcggtga agctcgacaa gggagagttt gtcggcagag aggcgctgct
     1621 gaggcgcaaa gaggaaggcc cgcggcgcag actttcgtgt ctcctcctcg acgacccgag
     1681 cgtggtcgtc atgggcagcg agccggtcta cgccgagggc agaccggtcg gctacgtcac
     1741 cagtgcggct tacgggtact cgatcgtaca gagcatagcc tacgcttggc taccgcccgg
     1801 actctcggag gagggcgcga aggtggagat cgaatacttc ggtaagcgcc atggcgcgac
     1861 cgtcgcggag gagccgctcc ttgacccggc catgaagcgt atgcggggct gaggaggcag
     1921 ggccgtgatg agcaccaagt atgcaagcgg gcatcgggtt gcggtctcca gcgtgctcga
     1981 cggtattggc gatacaccgc tggtgccggt cgagggtatc tactgcaagc tcgagtacct
     2041 gaacccctcg ggctccataa aggcgcgcat cgccaagtac atggtcgaga gggccgagag
     2101 ggagggtctc ctaaaacccg gcgacacgat agtcgaggct tcgagcggca acaccggcaa
     2161 cgccctctcg atggtggccg ccgtaaaggg ctacaggatg ctcgtggtca tgcccgaggg
     2221 gatgtccagc gaacgcgccg tcataagccg cgccttcggc gccgaggtct tagaagttgg
     2281 gcacttccac gtcaacgagg cgctggccaa ggcgaagaag ctcgggcggc aggacggcta
     2341 cttctgtccc tctcagttcg agtcggagtg gaacgtcgag gagaaccgcg tgtggctcgg
     2401 gccagaaatc ctctctcagc tccccgaagg gactgtcccc gacgcactcg tcatgggtgt
     2461 aggcacagga ggcaccctgg tgggcgtcgg ccaggccttc cgcgcggtta acccggacgt
     2521 aaagctcttc gccgtcgaac cggacgagtc gtgcaccatc ttgtgcggcg aaacgggcaa
     2581 acacgccata gaaggcatct ccgatggatt cgtacccggc atcttcgagc ggcatggggg
     2641 attggtggat ggcgtgcttt cc
//
LOCUS       DASTUV010000299         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_468301, whole genome shotgun sequence.
ACCESSION   DASTUV010000299 DASTUV010000000
VERSION     DASTUV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468301"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFT03_06015"
     CDS             <1..785
                     /locus_tag="VFT03_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU4847735.1"
                     /translation="PTGGIKILLLLKRSAGSGNFSPPRGGRTVRRVIILLAVITIAVA
                     AVGGVALAKTIRCHGGNCFGTNRADTIFGTNRHDAIFARGGGDFVTGRERNDNLNGQN
                     GNDSAFGGRANDWVKGGRGNDVVKGDLGNDRLTGGSGHNTIRAGDGMRDLIICGNHSR
                     NFIYFDPRLDRFRNCHFIRRLQVGAKGGSPRTASIIGLGPSQGSGSVSSTSSSVIGAS
                     TRTGSTAFVRTGSLRFGADPLDAGPRQKLVAVKVSAGVAHLK"
     gene            complement(967..1533)
                     /locus_tag="VFT03_06020"
     CDS             complement(967..1533)
                     /locus_tag="VFT03_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847736.1"
                     /translation="MMPTLACDVDSTIWNTGARVCEVVLEVTGDTLDVESVSTWTHLL
                     DAYGEETTTEIFDRVLSPQRIRDRELYPDAADVLRRLQGERGMKVHFVTHGDAVMEPH
                     LDAWLREHFGQGVGITITAGDKLAVLREIEAFGLVDDRSETLERVADAGLWAAARIQP
                     WNRELVAARADVHGFCDWREVPDLLPVL"
     gene            complement(1530..2162)
                     /locus_tag="VFT03_06025"
     CDS             complement(1530..2162)
                     /locus_tag="VFT03_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847737.1"
                     /translation="MSGPELRLDELPRPPLFKLAGEDGEDVFGETPVGGEPSKVGALF
                     SSRDIAEEFTAKAGAFGLEALAGLVPVELADWSAVEVYAASGQDYVLVVSGAGTGLFH
                     AGDLAHRAAEEEVPFPLYVISDEIGESPLISVEREEVLVAPLFSSPERARAFREQASH
                     LDLPDRLGSIEDREGLRRHALVARQAGAGYAVVDPVSGLTEAIPIEELIR"
     gene            complement(2159..2299)
                     /locus_tag="VFT03_06030"
     CDS             complement(2159..2299)
                     /locus_tag="VFT03_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEU4847738.1"
                     /translation="MTKAIVIGGGMGGLAAAAALGRAGVEVAVFERAPELKYEKIARY
                     RT"
BASE COUNT          423 a          807 c          731 g          394 t
ORIGIN      
        1 gtcccaccgg tggtattaag atactcttgt tgcttaagcg gagcgcggga agcggcaact
       61 tctcgcctcc gagaggaggt cggacggtga ggcgagtgat catattgctt gcggtgatca
      121 cgattgcggt ggccgcggtc ggcggggtgg ccctagcaaa aaccatacgt tgtcacggag
      181 gcaattgctt cggcacgaac agggccgaca ccattttcgg cacaaacagg cacgacgcca
      241 tattcgcgag gggtggcggc gactttgtga ccggccggga gaggaacgac aacctgaacg
      301 gccagaacgg caatgatagc gccttcggcg ggcgcgcgaa cgactgggtc aagggcggca
      361 ggggcaatga tgtggtgaaa ggcgacctgg gcaacgacag gttgacggga ggatccggcc
      421 acaacaccat aagggccgga gacggcatga gggacctcat catctgcggc aaccacagca
      481 ggaacttcat ctatttcgac ccgcgtctgg accggttccg caactgccac ttcattcgcc
      541 gtctccaggt aggtgccaag gggggatcgc cgaggacagc ttcgatcatc ggcctgggtc
      601 cctcccaggg ttccgggtcc gtaagctcga cgagttcgtc cgtgataggt gcgtctactc
      661 gaacggggtc gaccgctttc gttcggaccg gctcattacg ctttggcgct gatccgctcg
      721 atgctggacc acggcagaaa ctcgtagccg tgaaagtctc cgctggggtc gcgcacctca
      781 agtagcagac cgttaccgtc caggtcgcac acgtaacccg cgaggtcgtt gccgatcctc
      841 gccgagatcg agacgaagct cccccggctc aggaacccgg ggatctccgg agctccttcg
      901 cacatccctc cgttcctctc gccttccacc gcgcctcctc aaggatctcg ccttccgtgc
      961 aacagattac agcacaggca gcagatcagg aacctcgcgc cagtcgcaga agccgtggac
     1021 gtcggccctc gccgcgacga gctcgcggtt ccacggctgg atcctggccg ccgcccacag
     1081 acctgcgtcg gcgacgcgct cgagggtctc ggaacggtcg tccaccaggc cgaaggcttc
     1141 gatctcccgc aagacggcca gcttgtcccc ggccgtgatc gtgatcccga caccctggcc
     1201 gaagtgctcc ctgagccagg cgtcgaggtg cggctccatg acagcgtcgc cgtgggtcac
     1261 gaagtgaacc ttcatgccac gctcgccctg caaacggcgt aacacatccg cagcgtcggg
     1321 gtaaagctcg cggtcgcgga tcctctgtgg ggagagaacg cggtcgaaga tctccgtcgt
     1381 cgtctcctcg ccgtaggcgt cgaggaggtg cgtccaggtg gagacggatt cgacgtcgag
     1441 cgtatcgccc gtgacttcga gtaccacctc gcataccctg gcgcccgtgt tccagatagt
     1501 cgaatcaacg tcacaggcca acgtcggcat caccggatca gctcctctat cgggatcgcc
     1561 tctgtgaggc ccgacacagg gtccacgacg gcgtagccgg ccccggcctg cctggccacg
     1621 agggcgtgcc gcctgagccc ttcccggtct tctatgctcc cgagacggtc gggcaggtcc
     1681 aggtgggatg cctgctccct gaaagcgcgg gccctctcag gcgagctgaa gagcggggcg
     1741 acgagcacct cctcgcgctc gaccgagatc aggggcgact cccctatctc gtcgcttatg
     1801 acgtaaagag ggaaggggac ttcctcctcc gccgcacgat gcgccaggtc tccggcgtga
     1861 aagagccccg tgcccgcccc ggagaccacg agcacgtagt cctgtccgga ggcggcgtat
     1921 acttcgaccg cgctccagtc cgcgagctct acgggcacca gaccggcgag cgcctccagc
     1981 ccgaaagccc cggccttcgc tgtgaactcc tcggcgatat cccggctcga gaacagggcg
     2041 ccgaccttcg acggctcacc gcctacaggc gtctccccga aaacgtcctc gccatcctct
     2101 ccggccagct tgaagagtgg cggccggggc agctcgtcca gacgcagctc tggcccgctc
     2161 acgtcctgta ccttgcgatc ttctcgtact tcaactccgg cgcccgctcg aacaccgcga
     2221 cctccacgcc cgcgcgcccg agcgcggcgg ccgccgcaag ccctcccata ccgccgccga
     2281 tgactatagc cttcgtcacc agaggtcccc cgcttcgagc accgagccgg ccgggtcagg
     2341 cagcggcatg ccgtg
//
LOCUS       DASTUV010000300         4631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_824373, whole genome shotgun sequence.
ACCESSION   DASTUV010000300 DASTUV010000000
VERSION     DASTUV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824373"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFT03_06035"
     CDS             complement(<1..161)
                     /locus_tag="VFT03_06035"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEU4847739.1"
                     /translation="MENGYRHGRGFTLWFTGLSGAGKTTIADIVERELRLRHGKVEVL
                     DGDIVRTNLS"
     gene            complement(192..1409)
                     /gene="sat"
                     /locus_tag="VFT03_06040"
     CDS             complement(192..1409)
                     /gene="sat"
                     /locus_tag="VFT03_06040"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEU4847740.1"
                     /translation="MNVDIEYTTSIPHGGTLVDRRAPEDEREERTRRAEGLRGIAVGA
                     RTLSDLEMISTGVFSPLTGFMVREDYESVVDSMRLANGLVWSMPITLSVSEDEAREIG
                     EGEEIALATGDGQIVATMVVEDRYSYDKEREALEVYRTTDQDHPGVAALHRQSDTLLG
                     GEVTLLVDEPNPRPFPGYYYEPHELRKTFAQKGWKKVVGFQTRNPVHRAHEYIQKSAL
                     ETVDGLLLNPLVGETKSDDIPADVRMRSYEVILEQYYPTERTMLAVFPAAMRYAGPRE
                     AIFHAICRKNYGCTHFIVGRDHAGVGSYYGTYDAQHIFEEFDEDELGITPLFFEHAFY
                     CKECQGMASAKTCPHDPESHVFFSGTKVREMLGRGEYPPPEFSRPEVVKVLIEGMRDE
                     ERLIKQPRHYQEQ"
     gene            complement(1414..1746)
                     /locus_tag="VFT03_06045"
     CDS             complement(1414..1746)
                     /locus_tag="VFT03_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847741.1"
                     /translation="MDDARERLPLPGEPAGLRAETRALLEESPEEGARVVEDATFVAG
                     LLWEQWGETLERSGMDQARFLEISRGYSAELRLWVVGERPWNHCVSSLAGRVRRRLPA
                     PDGAASRR"
     gene            complement(1747..3054)
                     /locus_tag="VFT03_06050"
     CDS             complement(1747..3054)
                     /locus_tag="VFT03_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEU4847742.1"
                     /translation="MAGFDIRELMNDIEEAPGKVWFWDLERAVIDADRCVQCGVCVAA
                     CPTDSIGIGEDDLPTLVKMCTGCSLCWDFCPRGGLQYEATWKLGSARQQENNHSGAEP
                     GFELDPAHQNGSNGSGTGHDFKITGNGRPIDGIGRVHESFSARVDPMIEGAQDGGVVS
                     ALLISLLEAGEIDGALVARESEEEPWKGEAYLATTPEEVRECAGSFYNQTLALAHVDF
                     KGYELPPNPRIAVVGTPCEIEGIKALQARPWTWGSSQVEAITLTIALLCTKSFNYEKL
                     MLEEVRDKRGVDLDEVGRVDVIRGKLIVQDRDGETIFEEPIRDFHGAALKGCDECADF
                     MGHAADISVGSVGSVDGYSSVLIRSEEGLEAFSHVREKLSTRSLDKPQALDKLDALDK
                     RVAFSSLKRAFDPDAPLFIDYEEHLSNYAGTDRAPVEHEAVRY"
     gene            complement(3057..>4631)
                     /locus_tag="VFT03_06055"
     CDS             complement(3057..>4631)
                     /locus_tag="VFT03_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HEU4847743.1"
                     /translation="AIIDTYSKDLAAMDEVPGEVERLKWVGIYPQRQGGDAFMMRIKV
                     PGGVLKPEQARVIGRIATDFANGPIPNPHFGNNFLDLTTRQDVQMHWIKMGDVPEIWR
                     RLEEAGITTVQACGDSARNVLCCPVSGLGDEEVIDAYPVAQAISNYFTGNREYANLPR
                     KFKMSVTGCLEDCAQAEINDIGMLPARLEDGTVGFNLRVGGGLSDGPRMASDIDVFVR
                     PEDAVEITRAIAQVYGELGNRENRWINRMRYLVQELGPEGFREELEKRVSVELVPAGE
                     DLTKRYRGDHVGVHSQKSRDGFAICPYYVGLNVPVGRMSGEQFEEAGRLAQEYGEEVR
                     LATDQNLIITGVRQERLDDLLAEPLLERYSPSPGAFERGVVACTGSEFCRFGIVETKI
                     RALEWAREMDRRVGDPGQDAVRMHFSGCSASCAQPQIGDIGFRGETAKKDGTLVEGVD
                     IGLGGSLGMDAAFIDWVEGAKPADEIPDALARIFERFKQERREGERFHEWARRQHNVE
                     LREILRGLAAVAGKRN"
BASE COUNT          729 a         1596 c         1388 g          918 t
ORIGIN      
        1 gacaagttgg tgcgtacgat gtctccgtcg aggacctcga ccttgccgtg ccttagacgc
       61 agctccctct cgacgatgtc cgctatggtc gtcttgccag ccccggagag acccgtgaac
      121 cacagcgtga acccgcgccc gtgtctgtat ccgttctcca tctccaccct cctgttcgtt
      181 ttctgcttta tttattgctc ctggtagtgt cgaggctgct tgatgagccg ctcctcgtcc
      241 ctcatgccct cgatgagcac cttgacgacc tccggacgag agaactctgg aggcgggtac
      301 tcgcccctgc caagcatctc cctcaccttc gtccccgaga agaagacgtg ggactccggg
      361 tcgtgcgggc acgtcttcgc ggaagccatc ccctggcact ccttgcagta aaaggcgtgc
      421 tcgaagaaaa gcggggtgat cccgagctca tcctcgtcga actcctcgaa gatgtgctgg
      481 gcatcgtagg tgccgtagta gcttcctacc cctgcgtgat ccctgcctac gatgaagtgc
      541 gtgcagccgt agttcttgcg gcagatggcg tggaagatcg cctcgcgagg gcccgcgtag
      601 cgcatcgcag cagggaacac agcgagcatg gtgcgctccg tggggtaata ctgctccagg
      661 atgacctcgt acgagcgcat gcggacgtcc gctgggatgt cgtcggactt ggtctcacct
      721 accaaggggt tcaagagcag cccgtccact gtctctagag cgctcttctg gatgtactcg
      781 tgggctctat ggacggggtt tctggtctgg aagcccacca ccttcttcca gcccttctgg
      841 gcgaaggtct tcctcagctc gtgtggttcg tagtagtagc cggggaaggg acgtgggttg
      901 ggctcgtcga cgaggagcgt aacctcgcct ccgaggagcg tgtcgctctg acggtgcagg
      961 gcggccacgc ccggatggtc ctggtcggtg gtgcggtaga cctcgagggc ctcgcgctcc
     1021 ttgtcgtaag agtagcggtc ctcgacgacc atggtggcga cgatctgccc gtcacctgtc
     1081 gcaagcgcta tctcctcgcc ctcgcctatc tctctcgcct cgtcttcgga gacggagagc
     1141 gtgatgggca tgctccacac caggccgttg gcgaggcgca tactgtcgac gacagactcg
     1201 tagtcctcgc ggaccataaa acccgtaagc ggcgagaaga cgcctgtgga gatcatctcc
     1261 aggtccgaga gcgtcctcgc gcctaccgcg atcccgcgca gtccctcggc cctccgtgtt
     1321 cgctcctcgc gctcgtcttc cggcgctctt cggtctacga gcgtcccgcc gtgcgggatg
     1381 ctggttgtgt actcgatatc aacgttcatc cgttcacctc cgggaagccg caccatctgg
     1441 ggcggggagc ctgcgccgta cacgtccggc gaggctcgat acgcagtgat tccagggacg
     1501 ctcgcccacg acccagagcc tcagctctgc ggagtagccg cgggagatct cgagaaagcg
     1561 ggcctggtcc ataccggacc gctccaaggt ctcgccccac tgctcccaga gcaggccggc
     1621 gacgaaggtc gcgtcctcta cgacgcgggc gccctcctcg ggggactcct cgagcagggc
     1681 gcgggtctcc gctctcaacc cggcgggctc gccgggcaga ggcagtctct ctcgcgcgtc
     1741 gtccaatcag tacctcaccg cttcgtgttc gaccggggca cggtccgtcc cggcatagtt
     1801 gctcaggtgc tcttcgtagt ctatgaacag cggcgcgtcc ggatcgaagg cccgcttgag
     1861 actgctgaag gccaccctct tgtccaaagc gtcaagctta tccaaagcct gcggcttgtc
     1921 aagtgaacgg gtggacaatt tttcgcgcac gtgcgagaaa gcctcgaggc cctcctcgct
     1981 ccggatcagg acgctcgagt agccgtcgac gctcccgacg ctccccacgg agatatccgc
     2041 cgcgtgtccc atgaagtcgg cgcactcgtc gcagcctttg agggccgcgc cgtggaagtc
     2101 ccgtatcggc tcctcgaaga tcgtctcccc gtctctgtcc tggactatca acttccccct
     2161 gatgacgtcg acccggccga cctcgtccag gtccaccccc ctcttgtcgc gcacctcctc
     2221 tagcatgagc ttctcgtagt tgaagctctt ggtgcacagg agggctatgg tgagcgtgat
     2281 ggcctctacc tgcgaggacc cccacgtcca gggccgcgct tgcagcgcct ttatgccctc
     2341 gatctcacac ggcgtcccga cgaccgcgat gcgggggttg ggcggcaact cgtacccctt
     2401 gaagtccacg tgcgccagcg cgagcgtctg gttgtagaag ctaccggcgc actcgcgcac
     2461 ctcttcgggc gtggtcgcaa ggtacgcctc gcccttccac ggctcctcct cgctctcgcg
     2521 agccacgagc gcaccgtcta tctctcccgc ttcgagcagg gagataagaa gcgcgctcac
     2581 caccccgccg tcctgggcac cctcgatcat ggggtccacg cgcgcggaga agctctcgtg
     2641 gacccgcccg atgccgtcta tggggcggcc gttgcccgtg atcttgaaat catgtccagt
     2701 gcctgagccg ttgctcccgt tctgatgcgc gggatccagt tcgaaaccag gttcagcacc
     2761 tgaatggtta ttctcctgtt gacgtgccga acctagtttc cacgtcgcct cgtactggag
     2821 accgccgcgc gggcagaagt cccagcacag ggagcagccg gtgcacatct tgacgagcgt
     2881 gggcaaatcg tcttcgccga tacctataga gtcggtaggg caggcagcga cgcacacgcc
     2941 gcactgcacg cagcggtcgg cgtctatgac ggcccgctcg aggtcccaga accagacctt
     3001 cccaggggcc tcctcgatgt cgttcatcag ctcccggatg tcgaagccag ccacagctag
     3061 ttcctcttgc ccgctacggc agcgagcccc cgcaggatct cccggagctc cacgttgtgc
     3121 tgcctgcgcg cccactcgtg gaagcgttcg ccctcgcgcc tctcctgctt gaagcgctcg
     3181 aagatccgag cgagggcgtc ggggatctcg tcggccggct tcgcaccctc gacccagtct
     3241 ataaaggctg cgtccatgcc taaagaccca cccagcccga tgtccacgcc ctcgaccagg
     3301 gtgccgtcct tcttcgccgt ctcgcccctg aagcctatgt ccccgatctg gggctgcgcg
     3361 cacgaggccg agcagcccga gaagtgcatc cgcaccgcgt cctgcccggg atctccgacc
     3421 ctgcggtcca tttcgcgagc ccactcgagc gcccggatct tggtctccac tatcccgaag
     3481 cggcagaact cactccccgt gcaagccacg accccgcgct cgaaggcgcc ggggctcgga
     3541 gagtagcgct cgaggagggg ctcggccaac agatcgtcga gccgctcctg gcgcacgccg
     3601 gtgatgatca ggttctggtc ggtggcgagc cgtacctctt ccccgtactc ctgcgcgagg
     3661 cgtccggcct cctcgaactg ctccccgctc atcctcccca ccgggacgtt gagcccaacg
     3721 tagtagggac agatcgcaaa tccgtcccta ctcttctggg aatggacgcc gacgtggtcg
     3781 ccgcggtagc gtttggtgag gtcctcgccc gcgggcacga gctccaccga gacgcgcttc
     3841 tcgagctcct cgcgaaagcc ctccgggcct agctcctgca ccaggtagcg catccggttg
     3901 atccaacggt tctcccggtt tccgagctcg ccatagacct gcgctatggc gcgtgtgatc
     3961 tccaccgcgt cctcgggccg cacgaagacg tctatgtccg aggccatcct cgggccgtcg
     4021 gagagaccgc ccccgacgcg caggttgaag cctaccgtgc cgtcttcgag tctcgcgggc
     4081 agcatcccga tgtcgttgat ctccgcctga gcgcagtcct cgaggcagcc tgtgacgctc
     4141 atcttgaact tgcgcggcaa gtttgcgtac tcgcgattgc cggtgaagta gttcgagatg
     4201 gcctgcgcca cggggtaggc gtctatgacc tcctcatctc cgagccctga gacggggcag
     4261 cataggacgt tgcgcgccga gtcgccgcac gcctggacgg tcgttatgcc tgcctcctcg
     4321 agcctgcgcc agatctccgg tacgtcaccc atcttgatcc agtgcatctg cacgtcctgg
     4381 cgcgtcgtga ggtcgaggaa gttgttgccg aagtgggggt tcggtatggg accgttggcg
     4441 aagtccgtgg cgatgcggcc tatgaccctg gcctgctcgg gcttcaggac cccgcccggc
     4501 acctttatgc gcatcatgaa ggcgtccccg ccctgcctct gcgggtagat gccgacccat
     4561 ttgaggcgct cgacctcgcc cggcacctcg tccatggccg cgaggtcctt cgagtatgtg
     4621 tctattatcg c
//
LOCUS       DASTUV010000301         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1054345, whole genome shotgun sequence.
ACCESSION   DASTUV010000301 DASTUV010000000
VERSION     DASTUV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054345"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1031)
                     /locus_tag="VFT03_06060"
     CDS             complement(135..1031)
                     /locus_tag="VFT03_06060"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU4847744.1"
                     /translation="MGETISQRLVDAVPFLDRIAEAVQPKVREAVDESGTTVRNALDG
                     KWLELPLHPALTDVPLGSWTAALVFDGLDAATDSRAIRNAADASLVMGILGAFGAAVT
                     GLSDWRYLSGGSRRMGVAHGLLNTIGLALNVTSLLLRASGRRNAGRLALLAGYSVSGM
                     AAHLGGELSYDYGLRVNRNAFVPEGPEDYVQVLDEEELPRNGTRKVEVDGVGILLSRS
                     SDGEVCAISSVCGHLGGPLEEGSREGDTVVCPWHGSRYDLCSGKVLESPAVFPQSHYE
                     TRVREGRIEVKAAEENVQAKVM"
     gene            complement(1113..2282)
                     /locus_tag="VFT03_06065"
     CDS             complement(1113..2282)
                     /locus_tag="VFT03_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU4847745.1"
                     /translation="MLTKTRRAEVRDAIVVTAAVWVLALALWLDVRFMGGILGRISTE
                     AMNVHGDFDTFWRSARAFWGGGKVYATGADLENLNPPFWVLLISPFGLLEPLVAYRFF
                     VLITLSITVAYLAWVADELRVRPAWAVIGGVMLIVSSPLLATLALGQIYPILAFGLVV
                     AWMADRRERHRISGIALGLVVAIKPTLVPVLLWPLVRRRWGAFWAAIVAGAAATLVGA
                     VVVGFGATLDWLRLLSESSASPYWDNASLPSAAARLFTDNPYAQHVATWAWTIPVAYA
                     LGIAAIAFTATRTRWDAEAGLWALVAAALLASPIAWHNYLVLLGPGILLLLARGRAAS
                     AFLLLALQAIPGQWPLLWNREGTVAATLALTLYLYILLVHWIVLLPRWKEPAGGG"
     gene            complement(2461..2550)
                     /locus_tag="VFT03_06070"
     tRNA            complement(2461..2550)
                     /locus_tag="VFT03_06070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2514..2516),aa:Ser,seq:cga)
     gene            3015..>3403
                     /locus_tag="VFT03_06075"
     CDS             3015..>3403
                     /locus_tag="VFT03_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU4847746.1"
                     /translation="MGGSPRRYAVLSIVAAVLTIGLKLGAYFLTGSVGLFSDAAESVV
                     NLVAAVAALWALLYASRPPDEEHAFGHDKAEYFSSGLESALILIAAVWIGITAWDRLM
                     DPQPLHNVGIGLSITLVAAAINGIVAL"
BASE COUNT          625 a         1123 c         1056 g          599 t
ORIGIN      
        1 gacgagtacg ccagggcgcg cggcgacctc cagaaggtct tcgagacgga cgccggacga
       61 ggtgtgcgtc gagccgccgc ggaggcgctg gagaccctgg agaagcgcta gctttaaggc
      121 ttcagacctg acgactacat aaccttcgcc tggacgttct cctcggcggc tttcacctcg
      181 atcctgcctt cgcgcaccct tgtctcgtag tgtgactgcg gaaagacggc cgggctctcc
      241 aggactttcc cgctgcacag gtcgtagcgc gacccgtgcc agggacagac caccgtgtcc
      301 ccctcgcggc taccctcctc cagcggcccg ccgaggtggc cgcagacgct ggagatggcg
      361 cagacctcgc cgtccgagga gcggctgagc aggattccca ccccgtctac ttcgaccttg
      421 cgcgtgccgt tccgcggcaa ctcttcttcg tccagtacct gcacgtagtc ctcgggacct
      481 tcgggaacga aggcgttccg attgacgcgc aggccgtagt cgtaggagag ctcacccccg
      541 aggtgcgcgg ccatgccgct gacggagtag ccggcaagca aggcaagacg cccggcgttg
      601 cggcgccctg aagccctgag gaggagcgag gtcacgttga gcgcgagccc gatcgtgttg
      661 aggagtccgt gtgccacgcc catcctcctc gaccctccgc tcaggtagcg ccagtcggag
      721 agccctgtca ccgccgcgcc gaaggcgccc agaataccca tcacgagcga ggcgtcggcc
      781 gcgttcctga tggccctgga atcggtcgcg gcgtcgagcc cgtcgaagac gagggccgca
      841 gtccaggagc ccaggggcac gtcggtgaga gcagggtgca gtggcagctc gagccacttc
      901 ccgtcgagcg cgttgcgcac cgtcgtaccg ctctcatcga cggcctcccg caccttcggt
      961 tggaccgcct cggcgatcct gtccagaaag ggcacagcgt ccacgagacg ctggctgatc
     1021 gtctctccca tctcttccgc ctttccacga ggtgacagta tccctgtacc cgaagaagga
     1081 tcatgccact cggtcccgat cacctagacc ctctacccgc ctccggccgg ctccttccag
     1141 cggggtaata gtacgatcca gtgcacgagg agtatataga ggtagagcgt tagcgccagc
     1201 gtcgccgcca ccgtaccttc ccggttccaa agcagcggcc actgccctgg gatggcctgc
     1261 agggcgagga gcaagaaagc tgaggcggcc cgcccgcgcg ccagcaagag caggatgccg
     1321 ggccctagca ggacgaggta gttgtgccag gcgatgggcg aagccagcag cgccgcggcg
     1381 acgagagccc acagacccgc ctccgcatcc cacctggtcc tcgtagcggt gaacgcgatg
     1441 gccgcaatcc ccagcgcgta agcgaccggt atcgtccatg cccacgtcgc gacgtgctgg
     1501 gcgtaagggt tatccgtgaa cagccgggcc gcagccgagg gaagcgaagc gttgtcccag
     1561 tatgggctgg cagacgactc cgagagcagc ctcaaccagt cgagcgtcgc cccgaatcca
     1621 accacgacgg ccccgacgag cgtcgccgcc gccccagcca ctatcgccgc ccagaacgcg
     1681 ccccaccgcc gccgcacgag cggccaaagg agcacaggga cgagcgtcgg tttgatcgcc
     1741 accacgagcc caagggcgat gccggagatc ctgtgtctct cacgccggtc tgccatccac
     1801 gccacaacca ggccaaaggc gagtatcggg tagatctggc cgagcgccag tgtcgcgagc
     1861 aagggcgatg agacgatcaa catcaccccc ccgatgaccg cccacgcggg gcggacccgc
     1921 aactcgtcgg ccacccaggc cagatatgcg acggtgatgc tcaacgtgat cagtacgaag
     1981 aagcggtaag cgaccagcgg ctccagtagc ccgaagggcg agatgagcag tacccagaaa
     2041 ggcgggttga ggttctcgag gtcggcgccc gtagcgtaga ctttccctcc cccccagaat
     2101 gccctcgccg aacgccagaa ggtatcgaag tccccgtgga cgttcatcgc ctcggtcgag
     2161 atcctgccca ggatcccacc cataaagcgc acatccagcc acagcgccag cgccagcacc
     2221 cataccgccg ccgtgacgac gatagcgtcc cgaacctccg ctctacgggt cttggtcagc
     2281 acacctccct tgttagcacg ctgcctttca ggcagattgt cggcatttca gcttttccct
     2341 ttgctccgag cctagctgag aagccgcctg gcggagccta aggcggcgtc ctgggagcga
     2401 ggccctaaaa gggccgaagc gtgctgacaa gcgacggcgt agccggagcg tgataaatcg
     2461 cggagagggg gggattcgaa cccccgacgc cgggattacc gacgtaacgg ttttcgagac
     2521 cgccgcattc aaccactctg ccacctctcc attactgagt ttatccacta gccctcgacc
     2581 cttcaaccat cccccatcat aggacttcct gttcattgag cttgtgctct acggttatgc
     2641 agccggcgag cgttcgccct ctcgcactgt gcccgggaat gggtctcgct cccacgaaca
     2701 tgagaaaagc ctgtatccaa gccggtttca acgggtttcg gcctcgtctg cgcgtacgat
     2761 aaagccgcac cgtattgggt ttttgtgtaa ccttcatgtt cacggttata tttgaggctg
     2821 tcggattaac gctggtgttg ggtgggacgt agtcggttct tcaaatggtc aagtggggtg
     2881 tcgtcgaggc gggagtgtat actttggcgc cgcgtggtag aaggtgtcta cagtgttctc
     2941 gtggagcaga agcttttggt ggcgagatct atgacgacta ttctttggcg ctcgtgacga
     3001 aagagaccgt tactgtgggt gggtcccccc gccgctacgc cgtgctgtcc atcgtagccg
     3061 cggtgctgac cattgggctc aagctgggag cctattttct tacggggtct gtgggtctgt
     3121 tctccgacgc ggccgagtcg gtggtcaacc tggtcgcggc ggtggcggcg ctgtgggctt
     3181 tgctgtacgc ctcccgtccg ccggacgagg agcacgcctt cgggcacgac aaggcagagt
     3241 acttctcgag cggcctcgag agcgcgctaa tattgatcgc tgcggtctgg atcggcatta
     3301 ccgcctggga ccggctcatg gacccgcagc ctttgcacaa cgtgggtatc gggctctcca
     3361 taaccctcgt cgcagcggcg atcaacggca tcgtggcact gat
//
LOCUS       DASTUV010000302         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_294852, whole genome shotgun sequence.
ACCESSION   DASTUV010000302 DASTUV010000000
VERSION     DASTUV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294852"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFT03_06080"
     CDS             <1..635
                     /locus_tag="VFT03_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4847747.1"
                     /translation="SSAPISVQYPQATGSALAFKMRGEDGVVLACGGEASTSEGDWHE
                     AMNFAGIHDLPIIFLVENNVYAISVPEPLQVAGSIANRAEGYGFPGHRVDGNDVLAVY
                     EVVREAVERARKGDGPTLIEAKTYRMTAHSSDDDDRLYREREEIEEWRQKDPIVRFEK
                     YLMENGLLDEERKEEISAEIKAEVAEASEYAEQAPFADPEEALERVYAEG"
     gene            640..1626
                     /locus_tag="VFT03_06085"
     CDS             640..1626
                     /locus_tag="VFT03_06085"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU4847748.1"
                     /translation="MAEKNLLQAIHEGLAEEMRSDETVMVMGEDVGRAGGVFRVTQGL
                     QEEFGSLRVMDTPLAESLIVGSAIGLSVNGMRPVAEIQFADFIPPAFDQIVSEAARFY
                     YRSNGAWNVPITIRAPYGVVPGGALYHSQSVEAYFCNTPGLKVVAPTFPADAKAMIKS
                     SIRDPNPVLFLEHKRTYRLIKEDVPDDDYEVPLSKARVHREGSDLTVVSYGLTLYMAL
                     QVAQKLSEEEGIESEIIEPRTLYPLDRETILDSAKKTGKFLVVSEANLTGSVSGEIAA
                     IVAREAFEWLDGPVMRLGAPDIPAAAFAKPMMDFFVPSAEKIESAMLELARY"
     gene            1639..>2576
                     /locus_tag="VFT03_06090"
     CDS             1639..>2576
                     /locus_tag="VFT03_06090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU4847749.1"
                     /translation="MATPVTMPQLGESVTEGTITRWLKAEGDEVEKDEPLAEVDTDKV
                     NAELPSPVAGKIEKLLVSEGTTVDVGTEILLVATDGEESGPVEAQPREDAAAEGPTEE
                     FPAADTEAQPAVENGGEKQRAVAEARDSRSEGNGRGTVEDAETLRLQRSSPVVRRLAE
                     ENDVDISEISGTGTGGRVTKKDIESYIEERGAEREVAAEARPEARPDRVEVYDGDRVV
                     ELTSIRRAIANRMAVSKAEIPHAWTLVEVDMTGLAALREKEKEAFSEREGVKLTYLPF
                     IVKAAVEALKEQPVLNSVWDGDRILLRKQINVGVAV"
BASE COUNT          515 a          788 c          898 g          375 t
ORIGIN      
        1 cctcctcggc gcccatctcg gtccagtacc cgcaggcgac cgggagcgct ctggccttca
       61 agatgcgcgg tgaggacggg gtcgtgctcg cctgtggcgg cgaggcctct accagcgagg
      121 gtgactggca cgaggcgatg aacttcgccg gcatccacga tctccccata atcttcctcg
      181 tagagaacaa cgtctacgcc atctcggtcc ccgagccgct gcaggtcgcc ggctccatag
      241 cgaatcgcgc cgagggttac ggcttccctg gccacagggt agacgggaac gacgtgctcg
      301 ccgtctacga ggtcgtcaga gaagccgtcg agcgtgcccg caaaggggat ggtccgacct
      361 tgatcgaggc caagacctac cgcatgaccg cccactcctc ggacgacgac gacaggctct
      421 accgcgagcg cgaggagatc gaggagtggc gccagaagga cccgatcgtg cgcttcgaga
      481 agtatctgat ggagaacggg ctcctcgacg aggagagaaa agaggagata tcggctgaga
      541 taaaggccga ggtcgccgag gcgagcgaat acgcggaaca agcgcccttc gccgatcccg
      601 aagaggcgct cgagcgcgtc tacgccgagg gttaggaaga tggccgagaa gaacctgctg
      661 caggctatcc acgaagggct cgcggaggag atgcgctccg acgaaaccgt gatggtgatg
      721 ggcgaggacg tgggaagggc cggcggggtg ttcagggtaa cccaggggct ccaggaggag
      781 ttcgggtctc tccgggtcat ggacacgcct ctggcagaga gcctgatcgt cggctccgcc
      841 ataggtctct ccgtcaacgg gatgcgcccc gtggccgaga tacagttcgc cgacttcatc
      901 ccgcctgcct tcgatcagat cgttagcgag gcagcccgct tctactaccg ctcgaacggc
      961 gcgtggaacg tgcccataac catccgcgct ccctacggcg tggtgccggg cggcgcgctc
     1021 taccactccc agagcgtcga ggcgtacttc tgcaacaccc ctggcctcaa ggtcgtcgcc
     1081 ccgacgttcc cggcggacgc gaaggccatg atcaagagct ccatccgcga ccccaacccc
     1141 gtcctgttcc tcgagcacaa gcggacctac cgcctcataa aagaggacgt gccggacgac
     1201 gactatgagg tccctctgag caaggcccgc gtccaccgcg aggggagcga cctgacggtg
     1261 gtctcctacg ggctgacgct ctacatggcg ctccaggtgg ctcagaaact ctccgaggaa
     1321 gaggggatcg agtccgagat catcgagcct cggacgctct accctctgga cagggagacg
     1381 atactcgact ctgcgaaaaa gacgggcaag ttcctggtcg tctcggaggc gaacctgacg
     1441 ggatcggtat caggagagat cgcggccatc gtcgcgcggg aggcgttcga gtggctcgat
     1501 gggccagtca tgcgcctcgg ggcgccggac atcccggctg cggcgttcgc caagccgatg
     1561 atggacttct tcgtcccttc ggccgagaag atagagtcgg cgatgctcga gctggcccgc
     1621 tactagagga gagagaaggt ggcgacaccc gtaacgatgc cccaactcgg ggagagcgtg
     1681 acagagggta ctataacccg ctggctcaag gccgaggggg acgaggtcga gaaggacgag
     1741 cccctcgccg aggtggacac ggacaaggtc aacgccgagc tcccatcgcc agttgcgggc
     1801 aagatcgaga agctcctcgt ctccgaagga accaccgtgg acgtcggcac cgagatcctg
     1861 ctcgtcgcca ctgacggcga ggagtcaggg cctgtggagg cccagccccg cgaggacgcg
     1921 gccgccgaag gtccgaccga ggagttcccg gccgcggaca ccgaggcgca gccggccgta
     1981 gagaacggcg gcgagaagca gcgcgctgtc gccgaggcgc gcgactcgcg aagcgaaggg
     2041 aacggccggg ggacggtcga ggacgcagag acgctgaggt tgcagcgttc ctcgccggtc
     2101 gtgaggcggc tcgccgagga gaacgacgtg gatatctcgg agatctccgg cacaggcacg
     2161 ggcggcaggg tgaccaagaa ggacatcgag agctacatcg aggagcgcgg agcggagcgc
     2221 gaggttgctg ccgaagcgcg gcctgaggct cgacccgatc gcgtggaggt ttacgacggc
     2281 gaccgcgtcg tggagctcac gagcatccgg cgcgcaatcg cgaaccgcat ggctgtaagt
     2341 aaggcggaga taccgcacgc ctggacgctg gtcgaggtgg acatgacggg tctcgccgcg
     2401 ctgcgcgaga aggagaagga agcgttctcc gagcgcgagg gagtcaagct gacctacctt
     2461 ccgttcatcg tcaaggccgc ggtggaggct ctcaaggagc agccggtcct caactcggtg
     2521 tgggacgggg acaggatctt gctccgcaag cagatcaacg tgggggtcgc ggtgga
//
LOCUS       DASTUV010000303         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_286163, whole genome shotgun sequence.
ACCESSION   DASTUV010000303 DASTUV010000000
VERSION     DASTUV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286163"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFT03_06095"
     CDS             complement(<1..330)
                     /locus_tag="VFT03_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847750.1"
                     /translation="MSAPMAVGSNAGPDRAPARPPSLAGLIPAAGFLLASFPLGIFWF
                     AALAAPILLFGFLAAGWLAVMALGSLWWTGNKQSRTLGRGLALIAAPLTALSVRGGRI
                     ERRRLSGS"
     gene            complement(384..1430)
                     /locus_tag="VFT03_06100"
     CDS             complement(384..1430)
                     /locus_tag="VFT03_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU4847751.1"
                     /translation="MGRHGYAAGALVGVLITGLALLFITVRTGEPPRIVNPWPLLAAL
                     GACVATWWLQGLIVAVLARPRLERLRLLDTFRVYMAGTFVAGISPVRGAEIPLEVYLL
                     RRLGLSAGEGSAVVGIRVLLDAAVLTPAALCGLALTSTLPGANTPGLLLAGLVILVAL
                     AILIFALGRRSSSVGSGRDWPGWARGGRARISGFLGDARRSVASYRGHRKSLAYAAAL
                     TIVYWAFRLCCGPLALMAVGWSGGWTPVIVAQLLLASFVLPLAPTPGGSGARELGLVA
                     LLSAHVPEGQLLSGIVVYSGLTYYLPVVVGALFAGRQLWLQVFPSKGGRPHIGERALR
                     RRPTVVPARSYNGD"
     gene            complement(1434..1907)
                     /locus_tag="VFT03_06105"
     CDS             complement(1434..1907)
                     /locus_tag="VFT03_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847752.1"
                     /translation="MERNTITRRQILRGAGLVGVGIMLGSSLGFAGARRGSEGGGAGS
                     ERPWYELGIIGEPVMDNQLLWYLSHTGQGTADIGECLDTAGRIDAADANSWPEEWLGT
                     AEEFSPGQAKKLYDALQCPKDYMLFTEEDTGLVHCQAGAPSVASQRMFDWLDEHI"
BASE COUNT          395 a          741 c          701 g          301 t
ORIGIN      
        1 ggaccccgag agacgccgcc gttcgatccg cccaccgcgt accgagagcg ccgtcagggg
       61 agcagcgatc agagctagcc cccggccaag cgtccggctt tgcttattgc cggtccacca
      121 cagcgatccg agggccatca cagcgagcca ccctgcggcc aggaagccga agagcaggat
      181 cggcgcggcc agcgcggcga accagaatat cccgagcggg aagctcgcca gcaggaagcc
      241 tgcggccggt atcagaccag cgagggacgg cggacgggcg ggagcgcggt ccggcccggc
      301 gttggagcca actgccatcg gtgcggacac tggatgcttc ctctctcgac tcacggtcgc
      361 cggggacgcg ccatcccggc agtctagtcg ccattatagg accgcgccgg tacgaccgtg
      421 ggacggcgcc gcagcgcgcg ctccccgatg tgcggccttc cacccttcga agggaagacc
      481 tgcagccaca gctgcctacc cgcgaacagg gcccctacga ccacgggcag gtagtaggtc
      541 agaccggaat agacgacgat gccgctcaac agctgcccct cgggcacgtg ggcggacagc
      601 agggccacga ggcccagctc ccgcgcgccg cttccgcccg gggtaggggc gagcgggagg
      661 acgaaagagg ccagcagcaa ctgcgccacg atcacggggg tccagccacc cgaccagccc
      721 accgccatca gcgccagcgg gccacagcag agacggaacg cccagtagac gatcgttagg
      781 gcagccgcgt atgccaggct ctttcgatgc ccccggtagg aggcaacgct cctccgtgcg
      841 tctccgagga agccggatat cctcgcccgt cctcctctcg cccaaccggg ccaatcacgg
      901 cccgacccga cgctactgga tctcctcccg agcgcgaaga tgaggatggc aagcgccact
      961 aagataacca accctgccag gagaagcccc ggggtattcg ccccgggaag cgtggatgtc
     1021 agggcgaggc cgcacagcgc ggccggggtc aggacggcgg cgtcgagcag aaccctgatg
     1081 ccgaccactg cgctcccctc ccccgccgag aggccgagcc gcctgagcag gtacacctcg
     1141 agagggatct ccgcccctct aacaggtgaa atgccggcta cgaacgtgcc agccatgtag
     1201 actcgaaacg tgtcgagcag ccgaagcctc tcgagccggg gccgcgccag cacggcgacg
     1261 ataagaccct ggagccacca ggtcgccacg caggctccca gggccgcgag gagaggccag
     1321 gggtttacga tccggggtgg ctcccccgtc cttacggtta tgaacagcaa cgccaggccg
     1381 gtgatgagga ctcctaccag tgctccggcg gcgtaaccgt ggcgacccat cggttagatg
     1441 tgctcgtcca gccagtcgaa catccgctgg ctggcgacgc tcggcgcgcc ggcctggcag
     1501 tggacgaggc cggtgtcctc ttcggtgaaa agcatgtagt ccttggggca ctgcaaggcg
     1561 tcgtagagct tcttcgcctg ccccggggag aactcctctg ccgtgcctag ccactcttca
     1621 ggccagctgt ttgcgtccgc cgcgtcgatg cggccggcgg tgtcgaggca ctcgcctatg
     1681 tccgccgtgc cctggcccgt atggctcaga taccagagca gctggttatc catgacgggc
     1741 tcgccgatga ttcccagctc gtaccaggga cgttcggatc cagcgcctcc tccctcactc
     1801 cctcgccgag cgccggcaaa gcccaggctc gagccgagca tgatgcctac cccgaccagt
     1861 cccgcgcccc gcagtatctg gcgcctggtg atggtattgc gttccaattc ttcgcttccc
     1921 tcctgggtga actcgtgatg gcctggacct tcgacgagat cgtaggagac ccgacccgcg
     1981 aggagaatca ggcgggcagg agactttcta gtagggttaa cctcaccttg cccgcgtcac
     2041 tggaccactg cgagggcgca cagggcgagg cagcatccgg ggccagattg tgcgagcatg
     2101 accggagaag acggcgtcgg gatggcggac cgggagga
//
LOCUS       DASTUV010000304         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1322716, whole genome shotgun sequence.
ACCESSION   DASTUV010000304 DASTUV010000000
VERSION     DASTUV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322716"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFT03_06110"
     CDS             complement(<1..435)
                     /locus_tag="VFT03_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4847753.1"
                     /translation="MKEWRGMVRARTLLALVGLVFVLVSGTAAGNAAGAQAKQERPDP
                     PKINAAAWALEDTETGLYLADKNPDKRLPIASTTKIMVALIAFDRGVDLDEEVTVSNQ
                     AERFVGSVYSNVGLISGERLSVRELLQAALIPSGTDAVYALAE"
     gene            648..1169
                     /locus_tag="VFT03_06115"
     CDS             648..1169
                     /locus_tag="VFT03_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847754.1"
                     /translation="MTTSGVEDDAWRLLGAFYDLSGHNPLEPVPIGTPDSPPNESAAK
                     TAGLEPGTPECDVAVKYLLNGRYIRETGTPGECQITVQGIEKVREMRGLAEPSKGGNR
                     MSEETQRRLLTVLAIGIAMVLSRPVDRYIDEHIPERRGIKDDLAEAALQGLVRAAAFF
                     VASLIVRLIAGAR"
     gene            1312..1962
                     /locus_tag="VFT03_06120"
     CDS             1312..1962
                     /locus_tag="VFT03_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4847755.1"
                     /translation="MKVLVAGAHGKTARRLVRMLTEDGHQVRGLVRKEEQMSDVESDG
                     AEPVLVDLEKEGVEGAVGRAVEGCDAVIFAAGAGPGSGAARKETMDYGGAVKLIIAAE
                     EHGARRYLILSSMGAGDPEGGSEAMRPYLRAKARADERLQESGLDYTIIRPGSLTEEE
                     GRGRIEAAEALGRRGEISRDDVARTFAEALEMENTYRKTFEILAGDTPIRDALQKI"
     gene            1966..>2149
                     /locus_tag="VFT03_06125"
     CDS             1966..>2149
                     /locus_tag="VFT03_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847756.1"
                     /translation="MAGRDSKRTRVAAATALLGGLLYAREVEPRRVEVRPVELRLPRL
                     APEFDGYRLVQFGDIHL"
BASE COUNT          391 a          656 c          761 g          341 t
ORIGIN      
        1 ctcggccagg gcgtagacag cgtcggtgcc cgaggggata agagcggcct ggaggagctc
       61 tcttacgcta aggcgctcgc cgcttataag ccctacgttg ctgtagacgg agccgacgaa
      121 gcgctctgcc tgatttgaga cggtcacctc ctcgtcgagg tcaactcccc tatcgaaggc
      181 tatgagggcg accatgatct tggtagtcga ggctatgggc agccgcttgt cggggttctt
      241 gtctgccagg tagagccccg tctccgtatc ctcgagcgcc cacgccgctg cgtttatctt
      301 cggcggatcg gggcgctcct gcttcgcctg cgctccggcc gcgttcccag cagcggtccc
      361 ggatacgagg acgaacacga gcccgaccag cgcaaggagg gtccgcgcgc ggaccatccc
      421 tcgccactcc ttcaccactc ctcgaccggg caagaaccac cccctcgggg ttgaccacgt
      481 gtaccttgaa accactcctc cggcggacct cgcctatttt gcacgaggta ggccggcctt
      541 accaccctca aggaaggcga cagcaacagg cggttacgtc gcgccgctgg cggggtacat
      601 ctgcagcgta cgcattgtga cctttcgcga agtagaggag gtgcggcatg acgaccagcg
      661 gcgtagagga cgacgcgtgg cgcctcctgg gagcgttcta cgacctctct gggcacaatc
      721 ccctcgaacc cgtcccgatc gggacaccag actcgccccc gaacgagagc gcggcgaaga
      781 cggcgggact tgagccgggg acgcccgagt gcgacgtggc ggtcaagtac ctgctcaatg
      841 ggaggtacat aagagagacc ggcacgccgg gggagtgcca gatcaccgtg cagggcatcg
      901 agaaggtaag ggagatgcgc gggctcgccg agccctcgaa aggaggaaac cggatgagcg
      961 aagagacgca gaggcggctc ttgaccgtgc tggcgatagg catagcgatg gtcctgtccc
     1021 gcccggtcga caggtacatc gacgagcata taccggagcg caggggcatc aaagacgacc
     1081 tggccgaggc cgctttgcag gggctggtca gggccgccgc gttcttcgtc gcctcgctca
     1141 tcgtgcgcct gatcgccgga gcacgctaga tcccagcttg tcagcacgcc gcctttcagg
     1201 cagcttgtca gcatttctgg tcctgccggg atcgattgga ggaatcgtgt ctacagtcgc
     1261 ggaatttgcg gggtttcttc gggctacggc attgaggaag ggagaccaac tgtgaaggtt
     1321 cttgttgcgg gggcgcacgg caagacggcg aggcggctgg tcaggatgct gacggaggac
     1381 ggtcaccagg tacgggggtt ggtccgcaag gaggagcaga tgtctgacgt cgagtccgac
     1441 ggcgcggagc ccgtgctggt ggacctcgaa aaggaaggtg tcgagggggc cgtcgggcgg
     1501 gctgtggagg ggtgcgacgc ggtgatcttc gccgccggtg caggtcccgg gagcggagct
     1561 gcccgcaagg agacgatgga ttacgggggc gccgtgaagc tcatcatagc ggcggaggag
     1621 catggcgcac gccgttacct gatactcagc tcgatgggcg ccggcgaccc ggagggaggc
     1681 tccgaggcga tgcggccata cctgcgcgcc aaggcccgcg ccgacgagcg gctgcaggag
     1741 agcgggctcg actacacaat catccgcccc ggtagcctca ccgaagagga gggaaggggc
     1801 aggatcgagg ccgcggaggc tctgggcagg cgcggtgaga tcagccgcga cgacgtcgcc
     1861 cgcaccttcg cggaggcgct cgagatggag aacacctacc gcaagacctt cgagatcctc
     1921 gccggagaca cccccatcag ggacgcttta cagaagatct aggtcgtggc cggtcgtgac
     1981 tcgaagcgga cgcgggtggc cgcggccacc gcgctcctgg gcgggctgct ctacgcgcgg
     2041 gaggttgagc ctcgccgggt ggaggtcagg cccgtggagc tcaggctgcc acggctggca
     2101 cccgagttcg acggctaccg gctggtccag ttcggagaca tccacctcg
//
LOCUS       DASTUV010000305         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_367109, whole genome shotgun sequence.
ACCESSION   DASTUV010000305 DASTUV010000000
VERSION     DASTUV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367109"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFT03_06130"
     CDS             <1..297
                     /locus_tag="VFT03_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847757.1"
                     /translation="ATLGPEADSPAVLLRLEGDPGTTFSGICVAGSKQSVLSGRIPKS
                     YAFDPRGRKLSCRLEKRDRGRGDLKVILVVGNTTRSVQQTDARGNVLKVSYPGN"
     gene            495..1058
                     /locus_tag="VFT03_06135"
     CDS             495..1058
                     /locus_tag="VFT03_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4847758.1"
                     /translation="MNREESWELLCEWTESDSLRKHMLAVEAAMRAYARKFGEDEEKW
                     GATGLLHDMDYEKHPTPAEHPMVGVAELEKRGYPEDVLHAIKGHADYLDVPRDTLMSK
                     TLYAVDELSGFIVAVALMRPERLEGMKAKSVRKKMKQKSFAAAVNRDDIVRGAEELGV
                     DLNEHIEFVAAALKERADALDLAPKGA"
     gene            1112..2167
                     /locus_tag="VFT03_06140"
     CDS             1112..2167
                     /locus_tag="VFT03_06140"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEU4847759.1"
                     /translation="MARVMVVFGGRSGEHEVSLASARAIMDALGGRHEVVPVGITREG
                     GWISSGDPMRELESRSGAMIGAGGASDTGLPAKSGQKIPAALDSADVVFPVLHGPYGE
                     DGTIQGMLELAGVPYVGSGVLASAVGMDKLTMKKIFAYHGLPQVEWLGLTRKDWEEDG
                     DGWVRHVEASLGFPCFVKPANLGSSVGIGKATDAVVLRSALDAAARLDRRLIVEAGVD
                     AREIEVSVLGNEKAEVSVPGEILLKKNEFYDYEAKYAEGGMELVVPAKIPDETADELR
                     RMARTAYEAIDAAGMARVDFFLERATDRLLINEINTIPGFTSTSVYSRLWAATGLPYE
                     ELLDRLVRLAIERHRER"
     gene            complement(2220..>2511)
                     /locus_tag="VFT03_06145"
     CDS             complement(2220..>2511)
                     /locus_tag="VFT03_06145"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU4847760.1"
                     /translation="ASLGTLGTLVYAVLAFAYFTLLEGYLGQTLGKMLLGIKVVREDN
                     GEVPGLGAAAIRTVLRLIDGIFAYLVGFIAIQISSKNQRLGDMAANTLVVRK"
BASE COUNT          509 a          757 c          853 g          392 t
ORIGIN      
        1 gcaactctag gcccggaggc ggattcgccg gccgtcctgc tccgtctcga aggggacccc
       61 gggactacct tctccggcat ctgcgtcgca gggagcaaac agagcgtcct gagcggccgg
      121 atacccaaaa gttacgcctt cgacccgcga ggacgtaagc tctcgtgccg cttagagaag
      181 cgagaccgcg gcaggggcga cctgaaggtg atcctggtcg tcggcaacac gacccgctcg
      241 gtgcaacaga ccgacgcgcg cggcaacgtc ttaaaagtct cctatccagg taactgagga
      301 ctagagcctg acgccgagaa cgacgcggac aacgacggca ccgaagacgc gcgatagcca
      361 gacgatcgga ctcacctagc cagagcgact gatccccgga agaagccggt gtcgtacccc
      421 cggtctctta cttatgggtg cgtgatctat aaaatatgcg gctgtagaga cgatcctttc
      481 ttctggggag ggctatgaac cgcgaagagt cctgggagct gctgtgcgag tggacggaga
      541 gcgactccct gcgaaagcac atgctcgccg tcgaggccgc catgcgcgcc tacgccagaa
      601 agttcggcga ggacgaggag aagtggggcg cgacagggct cctgcatgac atggactacg
      661 agaagcaccc gacgcccgca gagcatccga tggtgggggt cgcggaactc gagaagaggg
      721 gctatcccga ggacgtgctc cacgccataa agggccacgc agactacctg gacgtcccgc
      781 gcgacactct gatgtccaag accctgtacg cggttgacga gctctccggc ttcatcgtag
      841 ccgtggcgct catgcgcccc gagcgtctcg agggcatgaa ggcgaagagc gtgcgcaaga
      901 agatgaagca gaagtccttc gccgcggccg tgaaccggga cgacatcgtc aggggagctg
      961 aggaactcgg cgtagacctg aacgaacaca tagagttcgt ggccgcggcg ctcaaagaga
     1021 gagccgacgc tctggatctc gcgccgaagg gcgcttgaga gcacgggccc tctgctacga
     1081 tggatgcggt ggagaggaga ggagtagcgc attggctcgg gtaatggtcg tcttcggcgg
     1141 tcggagcggg gagcacgagg tctctctggc atccgcgcgc gccattatgg acgctctcgg
     1201 cggtcgccac gaggtagtac ccgtgggcat tacgagggag gggggctgga tctcctccgg
     1261 cgatcccatg cgcgagctgg aatccaggag cggggctatg atcggggcag gaggcgccag
     1321 cgacaccggt ctcccggcga agagcgggca gaagataccg gcggcgctcg actccgcgga
     1381 cgtagtcttc cctgtcctgc acgggcctta tggggaggac ggcacgatcc agggcatgct
     1441 cgagctcgcc ggcgtcccct acgtcgggag cggggtgctg gccagcgcgg tcgggatgga
     1501 caagctgacg atgaagaaga tcttcgccta ccacggcctc ccgcaggtcg agtggctcgg
     1561 cctcacccgc aaagactggg aagaggacgg ggacggctgg gtccggcacg tcgaggcctc
     1621 cctcggattt ccgtgcttcg tcaagcccgc gaacctcggc tccagcgtcg gcatcggcaa
     1681 ggccacggac gccgtggtgt tgagatcagc gctcgatgcg gctgccaggc tggaccggcg
     1741 attgatcgtc gaggccggcg tggacgccag ggagatagag gtctcggtgc tgggcaacga
     1801 gaaggccgag gtctcagtgc cgggcgagat cctcctcaag aagaacgagt tctacgacta
     1861 cgaggcgaag tacgccgagg gcgggatgga gctcgtcgtg ccggcaaaga tccccgacga
     1921 gacagcggac gagctccggc gcatggcccg caccgcctac gaagccatag acgcagcggg
     1981 gatggcccgc gtggacttct tcctcgaacg agccacggat aggctgctca taaacgagat
     2041 caacacgatc cccggcttca cttccaccag cgtctactcg aggctctggg ccgccaccgg
     2101 cctcccctac gaggagcttc tcgaccgcct cgtgcggcta gctatcgaac gccacaggga
     2161 gagatgaaag aggccggggc ggctagcccc agcccgatag tcggtgcttc tttcctggat
     2221 tatttgcgca ctaccagggt gttggcggcc atgtccccga ggcgctggtt cttcgaggag
     2281 atctggatcg caataaagcc gaccaggtac gcgaagatac catcgatgag gcgcagcacg
     2341 gtccttatgg ccgcggcgcc gagacctggc acttcaccat tgtcctcccg caccaccttt
     2401 atgccgagca gcatcttgcc gagcgtctgc ccgaggtagc cctcgagtag ggtgaagtag
     2461 gcaaaggcga gaacggcgta gacgagagtc cccagagtac cgagtgaggc g
//
LOCUS       DASTUV010000306         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_811302, whole genome shotgun sequence.
ACCESSION   DASTUV010000306 DASTUV010000000
VERSION     DASTUV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811302"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFT03_06150"
     CDS             complement(<1..756)
                     /locus_tag="VFT03_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU4847761.1"
                     /translation="MTDMADQKMQEYGFETLALHAGQDVDPHTTARAVPIYQSTSYVF
                     HDTDHAANLFALSEMGNIYTRIMNPTTDVFERRVAALEKGVGALATASGQAAETLAIL
                     NIMGAGDEIVSAAQLYGGTYNLFHYTLPKIGVDVKYVEGIDPEGFRAVISEKTRAVFI
                     ETVGNPALNTMDIEALAQVAHDAGVPLIVDNTLPSPYLVQPLEHGADIVVHSATKFIG
                     GHGTSIGGVIVDGGRFPWDNGKFPGFTEPDPSYH"
     gene            complement(781..1914)
                     /locus_tag="VFT03_06155"
     CDS             complement(781..1914)
                     /locus_tag="VFT03_06155"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU4847762.1"
                     /translation="MTLDPGAGARQAPARQGKLHHCIGYFEPELGGSLEDVALAYETW
                     GELDDTGENAVLIVHALTGDTHAAGEPDSVYRRGGWWSPMIGPGRAIDTGRNFVVCSN
                     VIGGCYGSTGPASPNPESGRPYGMRFPVVTIRDMVRTQKRLLDHLGVRRLRLVIGGSI
                     GGQQALEWAVEFPDFVEKAVPVAATNALGPQGLGMSELGRRAIMADPDWQGGDYYGTG
                     RSPDAGLAIARMAGMMTYQSAPGQWERFGRRPASRPARYEEFGGRFEIESYLHYQGRD
                     LVSRFDANSYLYLSRAMDLYDVAAGYDSEGEAYARIEADVLFVGISSDWLFPPGEVRA
                     TAGRAREAGADADYVEIDSLSGHDSFLKDWDALEAAVRPFLEG"
     regulatory      complement(2001..2119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2183..>2737)
                     /locus_tag="VFT03_06160"
     CDS             complement(2183..>2737)
                     /locus_tag="VFT03_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847763.1"
                     /translation="ASVEGLLPADRREDLETWINAKFAEGSELAAQDTVTVLRRGSSK
                     GPGVRCQVFATQYNGFTRYEVALPHRNLKDKFVARKKALRVIITRKRHRIRPPVKEVG
                     PWNIRDNYWQSRRKRDKFDSLRRCKPEAEAAYFNNFNHGKDQFGRKVLNPAGVDLTPR
                     VAKGLGLRRFQNAWVWVRYPWVGK"
BASE COUNT          441 a          909 c          860 g          527 t
ORIGIN      
        1 gtggtaggag gggtcgggct ccgtgaaacc ggggaacttg ccgttgtccc aaggaaagcg
       61 accgccgtcc acgatcacgc cgcctatgga ggtaccgtgg ccaccgatga acttggtcgc
      121 cgagtggacg acgatatccg ctccgtgctc gaggggttgg accaggtatg gcgacgggag
      181 ggtgttgtcc acgatgagcg ggacgcccgc gtcgtgggct acctgagcca gcgcctcgat
      241 gtccatggtg ttgagcgccg ggtttccgac ggtctcgata aagaccgccc tggtcttctc
      301 ggaaatgacg gcacggaagc cctcggggtc gatgccctcg acgtacttca cgtcgacccc
      361 gatcttggga agggtgtagt ggaagaggtt gtaggttccg ccgtatagct gtgcggccga
      421 gacgatctca tccccggcac ccattatgtt gaggatcgct agcgtctccg cagcctgacc
      481 cgaggcggtc gcgagcgcac ccacgccctt ctcgagtgcg gcgacgcgcc tctcgaagac
      541 gtcggtcgtc gggttcatga tgcgggtgta gatgttgccc atctcggaga gggcgaacag
      601 gttcgctgca tggtccgtat catggaagac gtaggaggta gactggtaga ttggaacggc
      661 ccgcgccgtg gtgtgtgggt ccacgtcctg cccggcgtgc agcgcgaggg tctcgaaccc
      721 gtactcctgc atcttctgat cggccatgtc ggtcatccgt ttctcccttc caacgtttct
      781 ctaaccttcg aggaacggcc tgacggcagc ctccagcgcg tcccagtcct tcaagaacga
      841 gtcgtggccg ctcaaggagt ctatctccac gtaatccgcg tccgccccgg cctctctcgc
      901 ccgaccggcc gtagcccgca cctcgccggg cgggaagagc cagtcgctcg agatcccgac
      961 gaaaaggacg tcggcctcga tccgggcgta ggcctctccc tcggaatcgt agccggccgc
     1021 gacgtcgtag aggtccatcg cgcgcgagag gtagaggtag gagttggcgt cgaagcggct
     1081 cacgaggtcc ctgccctggt aatggaggta gctctcgatt tcgaaccgcc cgccgaactc
     1141 ttcatagcgc gccgggcggc tcgcgggtct ccgcccgaac cgctcccact gcccgggggc
     1201 gctctggtag gtcatcatcc ctgccatgcg ggcgatggcg aggccggcat ccggcgagcg
     1261 ccccgtcccg tagtagtcac cgccctgcca gtcggggtcg gccattattg cgcggcgccc
     1321 gagctcgctc atgccgagtc cctggggtcc taaagcgtta gtcgccgcca cgggtactgc
     1381 cttctcgacg aagtctggga actcgacagc ccactcgagc gcctgctgtc cgccgatgga
     1441 acccccgatc acgagccgca gccgccttac tcctaggtgg tccagaaggc gcttctgcgt
     1501 cctgaccatg tccctgatgg ttaccacggg gaagcgcatg ccgtaagggc gcccgctctc
     1561 ggggttgggg gaggctgggc ccgtgctccc gtagcaccct cctatcacgt tcgagcagac
     1621 gacgaagttc cgccccgtgt ctatggcgcg tcccggaccg atcatcgggc tccaccaccc
     1681 gccgcgcctg tacacggaat ccggctcccc ggcggcgtgg gtgtcgcccg taagggcgtg
     1741 gacgatgaga acagcgttct cgccggtgtc gtcaagctcg ccccaggtct cgtaggcgag
     1801 cgctacgtct tccagcgagc cgccgagttc cggctcgaag tagcctatgc agtggtggag
     1861 tttgccctgt cgggccggag cctgcctagc cccggcccca gggtctagtg tcatactcgg
     1921 tagtaataca gcctccttca ctcgtcgggt gctacgcctt acgcgcaacc cgctacgcta
     1981 gaagaggccg tctgcctctc cccacatctc ccaggacctg gggtcctgct ggacttggca
     2041 cctcacggcg aaaagctagt ctcgccgccg gttgccgcgg tttctcaggg ccagttccct
     2101 ccaccgctct agatgcgagt tggcgctagg ttaaggatgc taaaacaccg ccccgcccgg
     2161 cgtcaagggc gaaaggctcc tcctacttgc caacccaggg gtagcgcacc cacacccagg
     2221 cgttctggaa cctcctgagc ccgagcccct tggcgacccg cggcgtaagg tccacgccag
     2281 ccgggttgag gaccttcctg ccgaactggt ccttgccatg gttgaagttg ttaaagtagg
     2341 cagcctcggc ctcgggcttg cagcgcctga ggctgtcaaa cttgtcccgt ttcctgcgcg
     2401 actgccagta gttgtcccga atgttccagg gcccgacctc cttgaccggg ggcctgatcc
     2461 gatgcctctt gcgggtgatg atgaccctga gagctttctt cctagcgaca aacttgtcct
     2521 tgaggttcct gtgcggcaga gcgacctcgt acctggtgaa gccgttgtac tgggtggcga
     2581 acacctggca cctcacaccg ggtcccttgg aggacccgcg tcgaagaacc gttacggtat
     2641 cctgcgcggc cagctcgctc ccctcggcga acttggcgtt gatccaggtt tcgagatcct
     2701 ccctccggtc cgcgggcaac aagccctcaa cggaagc
//
LOCUS       DASTUV010000307         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_115039, whole genome shotgun sequence.
ACCESSION   DASTUV010000307 DASTUV010000000
VERSION     DASTUV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115039"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFT03_06165"
     CDS             <1..428
                     /locus_tag="VFT03_06165"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847764.1"
                     /translation="EEALLEYRGTMFFISHDRYFLRKLATRVVDLEGRKLTNYLGGYD
                     YYRSHRRLDARDGKRRPRRRVRPGQSTEESVLASRLVAVEGEIDATERRLSRLEKELA
                     TSELYADGKRSREVVLEHRQLKNALEALYADWGELLEET"
     gene            complement(647..720)
                     /locus_tag="VFT03_06170"
     tRNA            complement(647..720)
                     /locus_tag="VFT03_06170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(684..686),aa:Pro,seq:cgg)
     gene            complement(876..1946)
                     /locus_tag="VFT03_06175"
     CDS             complement(876..1946)
                     /locus_tag="VFT03_06175"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU4847765.1"
                     /translation="MRALRRGDRGREVVDLQTRLQALGHDLGGRGIDGVFRAGTELAV
                     KAFQQSLGQLADGLVGPVTWREIVEAGYRPGGRLLYLRQPPFRGADVVALQRMLNDLG
                     FDPGAVNGLFDARTARAVRDFQKNAGLTDDGVVDAGVFKVLDTYAAQTLGTHPFPDKS
                     GGYFPEDLFSGAIVVDAAHGGRDTGYVTAKGFSEANLNLAVAHELAQILPAREVLLTR
                     SDDEEVSAFDRAFLANSSGAKMIVSIHHAAHRSPAACGSVSYYFERLGYHSHRGRMAA
                     IYLQRGISRTLGTCDIGEFGRSYEILRETNIPAVILEPLHLTSPRDFKLASDPYYPAT
                     VAEAIAGALELYAGRDTAFIAV"
     gene            complement(2074..2340)
                     /gene="groES"
                     /locus_tag="VFT03_06180"
     CDS             complement(2074..2340)
                     /gene="groES"
                     /locus_tag="VFT03_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU4847766.1"
                     /translation="MKFKPLGDRALVKLVEREETTPSGIVLPDTAKEKPQTAEVVAIG
                     DGDEVKVSEGDVIVFAKYSGTEIILDGDDYLVLDADDILGVVEE"
     gene            complement(2460..>2760)
                     /gene="trxA"
                     /locus_tag="VFT03_06185"
     CDS             complement(2460..>2760)
                     /gene="trxA"
                     /locus_tag="VFT03_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4847767.1"
                     /translation="EVTDATFESDVLKSEKPVIVDFWAPWCVPCRRVSPILEQISEER
                     NDVSFVKINVDDNPSTAMNYSISSIPTIARFEGGQVTQQAIGALPKNQLSAQLGL"
BASE COUNT          483 a          891 c          881 g          505 t
ORIGIN      
        1 tcgaggaggc cctcctcgag taccgcggca ccatgttctt catctcccac gaccgctact
       61 tcctgcgaaa gcttgccacg cgcgtcgtgg acctggaggg caggaagctc acgaactacc
      121 tcggcggcta cgactactac cgctcgcaca ggcgactcga tgcgagagat ggcaagcgcc
      181 gcccccgccg gcgggtccgt ccgggccaga gcacagaaga gagcgtgctc gcatcacgcc
      241 tcgtcgcggt ggagggcgag atcgacgcca ccgagcgccg cctgagccgc ctggagaagg
      301 agctcgccac ctccgagctc tacgccgacg gtaaacgctc gcgtgaggtc gtactcgagc
      361 accgccagct caagaacgcc ctcgaggcac tctacgcgga ctggggcgag ctactcgaag
      421 aaacctagca cgcctgtgct tcgcaggctt gtcagcacgc tacgcccttc gggcttcgct
      481 tgtcagctta aaagcaagga ggacgcgaca tggtgctccc ctgcgccagt ccaactcaaa
      541 gagtgtcttt ccaagcgtga ccgggcaagt gccgaacggc agagagtaag ctgaaatgct
      601 gacaagcgac agccgaaggc tggagcgtgc tgaattgcta aaaaagtcgg gacgagagga
      661 tttgaacctc cgacctcctc gtcccgaacg aggcgcgcta ccaggctgcg ctacgtcccg
      721 actgtgtcac tgattatatc agctaggttt caggctacag gtttttatga agagtcgcat
      781 tctcgtcggc cgttgcatta ccgataagta ccgggcgtca gctccagacg gcttacaaac
      841 gcggacacgg gaagcgccat gcgcttacct gatgcctaca cggcgatgaa agccgtatcg
      901 cggcccgcgt acagctcgag ggcgccggcg atagcctctg ctacggtggc agggtagtag
      961 gggtcggagg cgagcttgaa gtccctgggg ctggtgaggt gcaagggctc gagtatgacg
     1021 gcggggatgt tcgtctcgcg cagtatctcg taggagcgcc cgaactcccc gatgtcgcag
     1081 gtgcccaggg tgcggctgat gccgcgctgg aggtagatcg ccgccatcct gccccggtgc
     1141 gagtggtagc cgagccgctc gaagtagtag gagacgctcc cgcaggcggc gggcgatcgg
     1201 tgggcggcgt ggtggatcga gacgatcatc ttcgcccccg acgagttcgc caggaaggcc
     1261 ctgtcgaagg ccgagacttc ctcgtcgtcg ctgcgggtca gcaggacctc gcgggcgggt
     1321 agaatctggg cgagctcgtg cgccacggcg aggttgaggt tcgcttcgga gaagcccttc
     1381 gcggtgacgt atccagtgtc ccggccgccg tgcgcggcgt caaccacgat ggccccggag
     1441 aagaggtcct ccgggaagta gcccccgctc ttgtcaggga acgggtgggt gcctagagtc
     1501 tgggcggcgt aggtgtcgag caccttgaac acgcccgcgt ctacgacccc gtcgtcggtc
     1561 aggccggcgt ttttctgaaa gtctcgcacg gcccgtgcgg tgcgggcatc gaagaggccg
     1621 ttcacggcgc cggggtcgaa ccccaggtcg ttgagcatcc tctgcagcgc caccacgtca
     1681 gctcccctga acggtggctg gcggaggtac agcagtcgcc cccctggtct gtagccagcc
     1741 tccacgatct cgcgccaggt tacgggtccg accagcccgt cagccagctg gcccagcgac
     1801 tgctggaagg ccttcacggc gagctcggtg ccggccctga agaccccgtc tatgccccgg
     1861 ccgccgaggt cgtgacccag agcctgcagg cgagtctgga ggtccacgac ctcccttccc
     1921 ctgtctcccc tacgcagcgc gcgcacgtcc ctagcccggc ctcccctggt tcgctacgat
     1981 gagccgcctc gttggatggt catccgctaa aggaggagcg ccgggcgtcg tctaaaggca
     2041 cccggcgctc cgtggaatcc tacggtacgg ttcctactcc tcgacgaccc ccaagatgtc
     2101 gtcggcgtcg agtaccaggt agtcatcgcc gtcgaggatg atctctgtcc ccgagtactt
     2161 ggcgaagact attacatccc cctcgctgac cttgacctcg tcgccatcac ctatggccac
     2221 gacctcagcg gtctgcggct tctccttggc cgtgtcgggc aggactatgc cggagggtgt
     2281 agtctcctca cgctccacga gcttgaccag cgccctgtcg ccgagtggct tgaacttcat
     2341 cccgagtacc tccttggatc ttcggactta cacacgattt acggcgcgat cccgctagac
     2401 tcattcggcc gccgggatca cccgcccgag attgtaaccg cgggcgttac ttgctgcaat
     2461 tacaggccga gctgggcgga gagctggttc ttgggcaggg cgcctatcgc ctgctgggtg
     2521 acctggccgc cctcgaagcg ggcgatggtc gggatgctcg atatgctgta gttcatcgcg
     2581 gtcgaggggt tgtcgtcaac gttgatcttg acgaagctca cgtcgttccg ctcctcggat
     2641 atctgctcga gtatggggga gacgcgccgg cacggcacgc accacggcgc ccagaagtcc
     2701 actatcacgg gcttctcgga cttgagaacg tcgctctcga acgtcgcgtc agttacttcg
//
LOCUS       DASTUV010000308         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1228426, whole genome shotgun sequence.
ACCESSION   DASTUV010000308 DASTUV010000000
VERSION     DASTUV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228426"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFT03_06190"
     CDS             complement(<1..293)
                     /locus_tag="VFT03_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847768.1"
                     /translation="MRALITDFDYEDVDIERRILEDAGFEVVAAQCRTGEDIVEAGRG
                     ASALLTQYAPITARVMDELPELRMVGRYGVGYDVVDVEAAGERGVWVANVPDYG"
     gene            complement(380..1009)
                     /locus_tag="VFT03_06195"
     CDS             complement(380..1009)
                     /locus_tag="VFT03_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4847769.1"
                     /translation="MKKTLRYSLAAVVVLGAGLVLQRRRPMMHPPRLTFLFENPIVNA
                     FVGPERLIQRLDLAPGMRVLDAGCGPGRITIPLARAVGPGGEVVALDGQREMLEKLQK
                     RLEAEGIGNVRPVRAVLGEGALGEGRFDRVVLAMVLGEVRDRREAARELYAALVPGGV
                     LSVTEIFGDPDHRRPSTIRREVEAAGFRLARSFGGFPAFTLNFEKPRTL"
     gene            complement(1425..1841)
                     /locus_tag="VFT03_06200"
     CDS             complement(1425..1841)
                     /locus_tag="VFT03_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847770.1"
                     /translation="MNLTYEYIRHRRGYSFAAGCCWIRIYEGEAGDAPVVVCEELPGV
                     GGAGVAEMAEQLAAEVILEHFTGGLPELPRPLLWVEHRPARRRGPGRYALLTFPSYHP
                     RQMGPGFVRRVTLGAPRREALSPEEVAILTGGEAGR"
     gene            complement(1868..2488)
                     /locus_tag="VFT03_06205"
     CDS             complement(1868..2488)
                     /locus_tag="VFT03_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU4847771.1"
                     /translation="MSVDPRLVVPYVIEQSPRGERSMDIYSRLLNDRIVFLGTPVDDQ
                     VANAIIAQLLHLDSEDPEQDINVYINSPGGSVTAGLAVYDTMRFVNADVATTALGMAA
                     SISAVLLAAGTKGKRNVLPNTRVLLHQPWVQGGPGGQASDVEIQARELVRTKRRMNEI
                     LAEATGQPFEKIERDTDRDYIMGAEEAVEYGVVDRVVGPKEGIAAG"
BASE COUNT          429 a          972 c          835 g          456 t
ORIGIN      
        1 ccgtagtcgg gcacgttggc gacccacacc ccgcgctccc cggcggcctc gacgtcaacg
       61 acgtcgtagc cgaccccgta gcggccgacc atgcgcaact cgggcaactc gtccatgacc
      121 cttgccgtta tcggggcgta ctgcgtcagc agcgctgacg ccccgcgccc ggcctcgaca
      181 atgtcctccc cggtgcggca ctgcgccgcc accacctcga acccggcgtc ctccagtatg
      241 cgacgctcga tgtccacgtc ctcgtagtcg aaatccgtta tgagtgctct caatttccta
      301 tcaccccgac gagccgacac tttccccagg ttcttacccg gaccggggcg aggtttacgc
      361 cagggctttc gagcgccgtc tagagcgtgc ggggcttctc gaagttgagc gtgaaggccg
      421 ggaagccgcc gaagctcctt gcgagcctga agcccgcggc ctcgacctcg cgccggatgg
      481 tggaggggcg cctgtgatcc ggatcgccga agatctcggt cacggagagt acgccgcccg
      541 gcacgagcgc cgcgtagagc tcccgggcgg cctccctgcg gtcgcgcacc tcgccgagca
      601 ccatcgcaag cacgacccgg tcgaaccgcc cctcccccag cgctccttcg ccgaggacgg
      661 cccgcaccgg tctcacgttc ccgatgccct cggcctccag ccgcttctgg agcttctcga
      721 gcatctcgcg ctggccgtcg agggcgacta cctctcctcc gggtcccacg gccctagcga
      781 gggggatggt gatccgccct ggaccgcagc ctgcgtcgag gacgcgcata ccgggggcga
      841 ggtcgagacg ctggatcagt cgctccggac ccacgaaagc gttcacgata gggttctcga
      901 acagaaacgt gagccgggga gggtgcatca tcgggcgccg ccgctgcagg acaagcccgg
      961 cgcctaacac caccacggcg gcgagcgagt aacgcagggt cttcttcatg tccctgagcg
     1021 tatcagttag cggcccatac cagcgagcgc cgcgtcctcg gcggcgtagt cgaaggagtc
     1081 gggatcagcg ttccgcggcg ggtgcgcacg ctctcactct acagtccgcc tcagcgcttc
     1141 ttcgcgaggg agctcctcct tgtagcccag ctcctgacgg tgctctggca ggcggtcgcc
     1201 ggggacggcc acgaccgatc tcgcgcatcc actccgccca cgaagggggg tcctgctccc
     1261 cgacgttgta cacgcggccg gcggcgcgct cgtcgctcac gccaaggact atggcggagg
     1321 cgacgtcagg cccctgtgga acacgacgac ctcgtggccg gctaaggcaa gccgccgcac
     1381 cacgtgcggg ccgatgaagt tcgtgccgcc gacgacgagg acccttatct tccggcctcc
     1441 ccgcccgtca ggatggcgac ctcctcgggg gaaagtgctt cgcggcgcgg ggcgcccaga
     1501 gtgacgcgcc gcacgaagcc cgggcccatt tgcctcggat gataggaggg gaaggtgagg
     1561 agcgcgtagc ggcccggacc ccgcctgcgg gccgggcggt gctcgaccca gagcagaggc
     1621 cgcggcagct ccggcagccc gcccgtgaag tgctccagga tcacctccgc cgcgagctgc
     1681 tccgccatct cggcgacgcc ggctccacct accccgggca actcctcgca gacgaccacg
     1741 ggggcgtcgc ccgcctcgcc ctcgtagatg cggatccagc aacaccccgc cgcgaacgag
     1801 tagcccctcc tgtggcgtat gtactcgtag gtaaggttca cctcaggctt cgctcccgcg
     1861 tcgcgcttta cccggccgcg atgccctcct tcgggcctac gacccggtcc acgacgccgt
     1921 actcgacggc ctcctccgcc cccattatgt agtccctgtc tgtgtcgcgc tcgatcttct
     1981 cgaagggctg tcccgtcgcc tcggccagga tctcgttcat gcgccgcttg gtgcgcacga
     2041 gctccctggc ctgtatctcc acgtccgaag cctgccctcc cggcccaccc tggacccacg
     2101 gctggtgcaa cagaaccctg gtgttcggga gcacgttgcg cttgcccttc gtgcccgccg
     2161 cgagcagaac ggccgagatc gaagccgcca tccctagagc cgtcgtcgcc acgtccgcgt
     2221 ttacgaaacg catcgtgtcg tagacggcga gccccgccgt cacactcccg ccaggcgagt
     2281 tgatgtacac gttgatgtcc tgctcggggt cttcgctgtc cagatgcaat aactgcgcga
     2341 tgatcgcgtt cgccacctgg tcgtcaaccg gcgtcccgag gaagacgatc ctgtcgttca
     2401 acaaccgcga gtaaatgtcc atcgaccgct ccccacgcgg gctctgctct ataacgtagg
     2461 gaaccaccag cctgggatcc acgctcatct cataacctcc tcaccagccg gcacgatata
     2521 cttggatagc gtagcactat tgtagtgttt tagcaagttt tttgtagtgt ttggaggtgg
     2581 cggcgatgga ccttccctgg ttacgggacc tacggcggcg ggctgtgatg agccaggagg
     2641 atctggcggc gaagagtggg gtagcgcggg acactatctc caagctggag ac
//
LOCUS       DASTUV010000309         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_543294, whole genome shotgun sequence.
ACCESSION   DASTUV010000309 DASTUV010000000
VERSION     DASTUV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543294"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFT03_06210"
     CDS             <1..842
                     /locus_tag="VFT03_06210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4847772.1"
                     /translation="DTADMYGPFTNEKLVGKAIQGRRDEVVLATKFGNERREDGSRVG
                     INGRPEYVRRACDASLQRLGVDTIDLYYQHRVDPEVPIEDTVGAMKELVETGKVRYLG
                     LSEAAPETIRRAHAVHPISALQTEYSLFTRDPEDEVLPTLRELGIGFVAYSPLGRGFL
                     TGAWKSMDDLPEDDTRAGRFPRFSEENFRQNLELAERVREIAAEKGATPGQLALAWLL
                     ARGDDIVPIPGTKRRKYLEENAGAVDVTLTGEDLRRIEEAIPRGSAAGERYSEQMMRT
                     VNR"
     gene            854..1453
                     /locus_tag="VFT03_06215"
     CDS             854..1453
                     /locus_tag="VFT03_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565683.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEU4847773.1"
                     /translation="MDDVEHISRKGSAVPLSWFPVPEETELPEDLQGLFRKAREKIGF
                     VPNVFRAYSFRPERLRAWFAHYRQLHEPTPNLDAAEREMIAVAVSMANGCLYCLVAHG
                     AALREALGDPILADRVTLDHRRAGLDERRTAILDFAVKVTERPLECTPQDLEQLEGFG
                     LTEEEVWDVVEIASMYNFTNRMSLACGMIPNEEYHALAR"
     gene            1460..1693
                     /locus_tag="VFT03_06220"
     CDS             1460..1693
                     /locus_tag="VFT03_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847774.1"
                     /translation="MLQAVSVLGALAILMAYAANQFGWISPSRLSYTLANLVGAGILT
                     AVALVERQIGFFLLQGGWTLVSLWGIVRILRAR"
     gene            1725..1946
                     /locus_tag="VFT03_06225"
     CDS             1725..1946
                     /locus_tag="VFT03_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847775.1"
                     /translation="MLILLAFAANQLGRIDTSNLSYQISNLAGSTILTTVAVIEAQLG
                     FILLEGAWALVSLWGTIKVARGESPGGAH"
BASE COUNT          365 a          686 c          719 g          325 t
ORIGIN      
        1 tagacacggc ggacatgtac ggcccgttca ccaacgagaa gctggtagga aaggcgatcc
       61 agggccggcg cgacgaggtc gtgctggcta ccaagttcgg caacgagcgg cgcgaggacg
      121 ggagccgggt tggcatcaac ggcaggcccg agtacgtgag gcgggcgtgc gacgcttccc
      181 tgcagaggct cggcgtggac accatagacc tctactacca gcacagggtt gacccggagg
      241 tgcccatcga ggataccgtc ggggcgatga aggagctggt cgagaccggg aaggtacggt
      301 acctggggct ctccgaggct gcgcccgaga caataaggcg ggcgcacgcg gtgcacccca
      361 taagcgcctt gcagaccgag tactcgctct tcaccaggga tccggaagac gaggtcctgc
      421 ctacgctgcg agagctgggc atcggcttcg tcgcctacag cccgctgggc cggggtttcc
      481 tcaccggcgc ctggaagagc atggacgacc tgccggagga cgatacccgc gcgggccgct
      541 tcccgcgctt ctcggaggag aacttcaggc agaacctgga gctggccgaa agggtgcggg
      601 agatcgccgc cgagaagggc gcgacgccgg ggcagctcgc cctcgcctgg ctactggccc
      661 ggggcgacga catcgttccc atccccggca ccaagcgccg caagtacctc gaggagaacg
      721 cgggggccgt ggacgtaacg ctgaccgggg aggacctgcg gcgcatcgag gaggcgatcc
      781 ccaggggctc ggcggcgggc gagcgctact ccgagcagat gatgcgcacc gtcaaccgct
      841 agaggagccc aggatggacg acgtagagca catctcgcgc aaggggagcg ccgtacccct
      901 gagctggttc cccgtcccgg aagagaccga gctgccggag gatttgcagg gcctcttcag
      961 gaaggcgcgg gagaagatcg ggttcgtccc gaacgtcttc agggcttaca gcttccgccc
     1021 cgagcgcctg cgcgcctggt tcgcccacta caggcagttg cacgagccca ccccgaacct
     1081 cgatgcggcg gagcgggaga tgatcgcggt cgccgtctcg atggcgaacg gctgcctcta
     1141 ctgcctcgtc gcccacggcg ccgcgctgcg cgaggcgttg ggagatccga tactggccga
     1201 cagggtaacc ctggatcatc gtcgcgccgg cctcgacgag cgccgcacgg cgatcctgga
     1261 cttcgccgtc aaggtcacgg agaggcccct cgagtgcacg ccgcaagacc tcgaacagct
     1321 cgagggattc ggcctgacgg aggaggaggt ctgggacgtg gtggagatcg ccagcatgta
     1381 caacttcacg aaccgaatgt cgctagcgtg cggcatgatc cccaacgagg agtatcacgc
     1441 gctggcgcgg taggtgctca tgctgcaggc ggtctcggtc ctcggcgcgc tcgccatcct
     1501 gatggcctac gccgccaacc agttcggctg gatcagccct tcccggctgt cgtacacgct
     1561 cgccaacctg gtaggcgccg gcatcctgac ggccgtcgcg ttagtggaac gccagatcgg
     1621 gtttttcctc ttgcagggcg gatggaccct ggtgagcctg tgggggatcg tcaggatcct
     1681 gcgcgcccgc tagcatgctg caggctgtct cggtcctcgg agccttgctg atactgctgg
     1741 ccttcgcggc caaccagctt ggcaggatcg acacctcgaa cctctcctac cagatctcca
     1801 acctcgccgg ctccaccatc ctcacaaccg tagccgtgat cgaggctcag ctcggcttca
     1861 tcctgctcga aggcgcctgg gcgctggtga gcctgtgggg gaccatcaag gtagcgcggg
     1921 gcgagagccc cggcggggcg cactagcctt gcctcagaca acctcgaggt tgatcttgcc
     1981 tacgccgccc cacacgtcga cgtcgagggt gacgtcggag tcgccgtagg cgtcgttcac
     2041 gtaggcgtcg tcctccatga gcgcgtcggc gccgcccgaa cccggcgtcg acgtc
//
LOCUS       DASTUV010000310         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_716499, whole genome shotgun sequence.
ACCESSION   DASTUV010000310 DASTUV010000000
VERSION     DASTUV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716499"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1523
                     /locus_tag="VFT03_06230"
     CDS             174..1523
                     /locus_tag="VFT03_06230"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4847776.1"
                     /translation="MDLREHVNNERDALLGELEEFLRMPSISARKDDGAGFRECAEWV
                     AGKLEEAGARATIMETEGHPVVYAEAGEGDRTLLSYGHYDVQPPEPLELWESKPFEPT
                     LRDGRLYARGVADDKGDVLARIQALRLYQEEHGPLPFKLKFFIEGEEEVGSPNLAPFV
                     RANAELLAADACLWEGSMKDGSGRPMIYCGTKGIAYVELRAKGASYDLHSMYGNIAPN
                     PSWRLIQALRTLKDEDGNVTLDRLDELADEPSEKDLEAIRNIPFDAEELKKSWGVESF
                     DRDLSGEEALREMLLEPSANIAGFMSGYTGPGSKTIVPSEAFVKMDFRLVAGQSPGQV
                     VELIRSHLRKRGFEDIEVVELHGVEAAKTPVDSPVVQTAVETWRDLGHEDAVVYPTIG
                     GSGPTSLIATELGIPTIMTGSVANTDSRLHSPNEWVRLDDYYEAISYFARFFERFGG"
     gene            1523..2104
                     /locus_tag="VFT03_06235"
     CDS             1523..2104
                     /locus_tag="VFT03_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847777.1"
                     /translation="MERPQGLDLPERVRHNIAAAVASRGVQARVISTRGRDSMRPPQG
                     HIIAAAEREAIDAVRGPARVVRRLEFYKTFYGPVVRLAFSVYPEDRERDLSAGTLLDV
                     SQVSGDAALSGLGRQKSIFVHFYYTEEGDLTYAFSKEVPNADEQRGEAKKVLKMARDA
                     YTETPEERRSFRAAVGLAERQFELPVAAPEEQA"
     gene            2146..3282
                     /locus_tag="VFT03_06240"
     CDS             2146..3282
                     /locus_tag="VFT03_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847778.1"
                     /translation="MGSTANDFVAKISKALTEAERKGIYVTAGAGSCDLEAQHFGRHP
                     SDEEEPMPAGRPHHLETPPGPRDPGSHDGTSRLCYFLDGVQSSREIGRIEMAPVIVAT
                     VAAAIVNRCERRFSRMPLESPPVVVQAVILPRSAGDAAVEAFWALLQEAGFSELGPDE
                     VPSSPHLILDSAQFVAEADPSDYVGMRERARVRVRALRERLEGGMLRQWEGDNRTLED
                     PDAWIAVDGQLENIRESNRRAVGLIKSVARPEFVGRDVGMLLDLEPGMRTTSFVPDWQ
                     LRRSPGDHRTSWYLRMWPPQRGADALGSLMRVEAPRDTKPEQIDEISRWILAERAPLA
                     KPDPRWPAMIYPIHYVEKILKPLVQGSERAYARLERQLAANGRN"
     gene            3283..>3542
                     /locus_tag="VFT03_06245"
     CDS             3283..>3542
                     /locus_tag="VFT03_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847779.1"
                     /translation="MLHPDYLDTGSGPIGRVVTSEEHPATAHEFYFWTAETDAAQSLD
                     IGHIVAAESEDATAIAVLDDPRRYSDLQSFLDDFYAFDGDPSL"
BASE COUNT          668 a         1174 c         1155 g          545 t
ORIGIN      
        1 gcctgtgaat gcggccatcg cggctttgct cggggctgtg atccggtctc ttctccaagg
       61 aggtgcctca ccccagaata taccagcaca tggggcagcg gaccacggtc gtcaatggca
      121 cgaaacaccg aagatgctac cctctcggaa ggtaggagaa aggacttgcg aggatggacc
      181 tgagagagca cgtgaacaac gagagagacg ccctcctcgg cgagctcgag gagttcctga
      241 ggatgccctc gatctccgcc cgaaaggatg acggcgcggg cttccgcgag tgcgccgaat
      301 gggtggcggg caagctcgaa gaggccggcg ccagagctac gatcatggaa accgaaggac
      361 accccgtagt ctacgccgag gccggcgagg gagacaggac cctcctctct tatggccact
      421 acgacgtgca gccccccgag ccgctggagc tctgggagag caagcccttc gagcccacgc
      481 tccgcgacgg taggctgtat gcccgcggcg tcgccgacga caagggcgac gtcctggccc
      541 gcatccaggc tctcaggctc taccaggagg agcatggccc ccttcccttc aagctcaagt
      601 tcttcatcga aggcgaagag gaggttggca gccccaacct ggcccccttc gtgcgtgcca
      661 acgccgagct gctcgcggcg gacgcctgcc tgtgggaggg ctcgatgaag gacgggtcgg
      721 gccggcctat gatctactgc ggcaccaagg gtatcgctta cgtcgagctg cgcgctaaag
      781 gcgcctccta cgacctccac agcatgtacg gcaacatcgc ccccaaccct tcctggcgcc
      841 tcatccaggc cctcaggacg ctaaaggacg aggacggcaa cgttaccctc gacaggctcg
      901 acgagctcgc cgatgaaccc tccgagaagg acctggaggc gataaggaat atcccgttcg
      961 atgcggagga actgaaaaag tcctggggcg tggagtcctt cgaccgcgac ctctcgggcg
     1021 aagaagcatt gagagagatg ctactcgagc cctcggccaa catcgccggc ttcatgtccg
     1081 gctatacagg cccgggctcg aagaccatcg tcccgtcaga ggccttcgtg aaaatggact
     1141 tccggctcgt cgcaggccag agccccggtc aggtggtcga gttgatccgg tcccacctga
     1201 ggaagcgcgg cttcgaggac atagaggtcg tcgaactgca cggggtcgag gcggccaaga
     1261 caccggtgga ttcccctgta gtacagacgg ccgtcgagac gtggcgagac ctcgggcacg
     1321 aggacgcggt cgtctacccc acgatcgggg gtagcgggcc cacctcgctc atcgccacgg
     1381 agctcgggat accgaccatc atgacgggga gcgtcgcgaa caccgacagc cgcctccact
     1441 cgcccaacga gtgggtgagg ctcgacgact actacgaagc gataagctac ttcgcccgct
     1501 tcttcgagag gttcggcggc tgatggagag accccagggg ctcgaccttc cggaacgcgt
     1561 gcggcacaac atcgcggccg ccgtcgcttc acggggcgtg caggcgcggg tgatctccac
     1621 gcgcggccgc gactcgatgc gtcctcctca gggccacata atcgccgccg cagagaggga
     1681 ggccatcgac gcggtccgcg ggccggcccg cgtcgtgcgc cggctcgagt tctacaagac
     1741 cttctacggt cccgtcgtcc gcctcgcctt ctccgtctac cccgaagaca gggagcggga
     1801 cctctcggcc ggcaccctcc tcgacgtctc gcaggtatcc ggcgacgccg cgctctccgg
     1861 cctcggccgc cagaagagca tcttcgtcca cttctactac acggaggaag gcgacctcac
     1921 ctacgccttc tccaaggagg ttcccaacgc cgatgagcag cggggcgagg ccaagaaggt
     1981 gctcaagatg gcccgcgacg cctacacgga gaccccggag gagcgccgca gcttcagagc
     2041 cgccgtcggc ctcgccgaga ggcagttcga gttgcctgta gctgcacccg aagaacaggc
     2101 atagtttctt tccagctatc cgcgtatact ttgttgtcaa tgctgatggg gagcacggct
     2161 aacgatttcg tagcgaagat aagcaaggcc ctcaccgagg ccgagcgcaa gggcatctat
     2221 gtaaccgccg gcgcgggatc ttgcgacctg gaggcccagc acttcggccg tcacccctcg
     2281 gacgaagaag agccgatgcc cgcggggcgt ccgcaccacc tggagacccc tccgggacct
     2341 cgggaccccg gcagccacga cggcacttcg cggctttgct acttcctcga tggcgtgcag
     2401 agctcgcgtg agatcggccg catagagatg gctcctgtca tcgtcgcgac agtagcggcc
     2461 gcgatcgtca accgctgcga gaggcgcttc tcgcgcatgc cgctggagag cccgcccgtc
     2521 gtggtgcaag ccgtgatact gccccgcagc gccggagacg cagcggtcga ggcgttctgg
     2581 gccctcctgc aagaggcggg gttctcggag ctcggaccgg acgaggtacc ctcctccccc
     2641 catttgatac tcgactctgc acagttcgtg gccgaggcgg acccttcgga ctacgtcggt
     2701 atgagggaga gggccagggt gagggtccgc gccctccggg aacggcttga gggcgggatg
     2761 ctcaggcaat gggaagggga caaccggacg ttggaggacc ctgacgcctg gatcgcggtg
     2821 gacgggcagc tcgaaaacat ccgggagtca aaccggcgcg ccgtcggcct cataaagagc
     2881 gtcgcccgtc ccgagttcgt tggccgggac gtggggatgc tgctcgattt ggagccgggg
     2941 atgcgcacga ccagcttcgt cccggattgg cagctcaggc gcagtccggg agatcacaga
     3001 acctcctggt atctgcggat gtggccgccg cagcgcggcg cggacgctct gggctcgctg
     3061 atgcgggtag aggcgccgcg ggatacgaag cctgagcaga tagacgagat ctcgcgctgg
     3121 atcctggcgg agcgggcccc gctggcgaag cccgacccgc gctggccggc catgatctac
     3181 cccatccact acgtggagaa gatcctcaag cccctcgtgc agggctccga gagagcgtac
     3241 gcgaggctgg agcggcaact cgcagcgaac gggaggaact agatgctgca cccggactac
     3301 ctggacaccg gctccggccc catcggaagg gtcgtgacca gcgaggagca ccccgcgaca
     3361 gcccacgagt tctacttctg gacggccgag accgatgcgg cccagagcct ggacatcggg
     3421 cacatcgtcg ccgccgagag cgaggacgcc accgccatcg cggtcctaga cgacccgcgc
     3481 cgctactcgg acctgcagtc cttcctcgac gacttctacg ccttcgacgg cgacccgtct
     3541 ct
//
LOCUS       DASTUV010000311         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1184901, whole genome shotgun sequence.
ACCESSION   DASTUV010000311 DASTUV010000000
VERSION     DASTUV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184901"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFT03_06250"
     CDS             complement(<1..737)
                     /locus_tag="VFT03_06250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU4847780.1"
                     /translation="MGAMLVDSHTHLLRLEVSPERAVRSAAEVGVVAVVNVGTDVEDS
                     QRGAELAAVLPNVYSTAGIHPHNAGTYTAADLEALAELSESPGIVALGEVGLDYYRSE
                     WSEEVQRALFEDVISLANDTRLPLVIHSREAFADTMACLGSARVPIVLHCFEGGAREV
                     REATERGYYVGLAGNVTYRDSETARVLRLIDEEHLLVETDAPYLSPQPVRGRKNAPEY
                     VVHTAGFVAERLGMEDVELATLTTRNAR"
     gene            complement(794..1594)
                     /gene="rsmI"
                     /locus_tag="VFT03_06255"
     CDS             complement(794..1594)
                     /gene="rsmI"
                     /locus_tag="VFT03_06255"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU4847781.1"
                     /translation="MPLTVIPTPIGNLEDITLRALRYLREADLVACEDTRRTGRLLAH
                     YDMKKNLLAYHEHNEERLAPELAERAQTERVALVTDAGTPLVSDPGHRLVRACIEAGV
                     EIEALPGPSALTTALVVSGLPADTVVFTGFPPRKGRGRTELLELVATEPSTFVLFESP
                     HRLGKTLGDLPARTPVAVCRELTKRYEEVFRGTAEEAANYFSGGAKGEIVLVVRGGTA
                     KTEESIEEAIRRARGYMSEGESPSRAAQKAARESVHRRSEIYERLVRG"
     gene            complement(1598..>2201)
                     /locus_tag="VFT03_06260"
     CDS             complement(1598..>2201)
                     /locus_tag="VFT03_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4847782.1"
                     /translation="VGLAADHLVFVALGTGIGGGVITHGILMRGAQGSGGELGHVTIQ
                     ATGPRCACGNHGCLEALASGTAIGRRARELAVEQPGSALGRLATRRQILGEDVTELAR
                     EGDEVALSVLDEAGKWLGVGLAGFVNIFNPEVVAVGGGVMEAGELVLRAARKEVILRA
                     RPPSRDLADVKVATLGPRSGVLGAAALARDASSGEYVLGG"
BASE COUNT          352 a          757 c          697 g          395 t
ORIGIN      
        1 cgggcgttac gggtggtcag ggtggccagt tccacatctt ccatcccaag cctctcggct
       61 acgaagcctg ccgtatgcac gacgtattcg ggcgcgttct tcctgccgcg cacgggctgg
      121 gggctcaggt aaggggcgtc cgtctccacg aggaggtgtt cttcgtctat caggcggaga
      181 accctggccg tctcgctgtc cctgtacgtt acgttgcctg cgaggccgac atagtagccg
      241 cgctccgtcg cctccctgac ctcgcgggca ccaccctcga agcagtgcag gacgatagga
      301 accctggcac tcccgaggca agccatcgta tcggcgaagg cttcgcgaga gtggatcacc
      361 agcggcaggc gcgtatcgtt ggcaagggag atcacatcct cgaacagcgc ccgctgcacc
      421 tcctccgacc actcagagcg gtagtagtcg agccccacct caccaagggc cactatcccc
      481 ggcgactccg agagctcggc cagcgcttcg aggtcggccg ccgtataagt acctgcgttg
      541 tggggatgta tacctgccgt cgagtagacg ttcggcaaca ccgccgcaag ctcggccccc
      601 ctctgcgaat cctcgacgtc ggtcccgacg ttcacaacag ccacgacccc gacctccgca
      661 gcgcttctta ccgctctctc cggcgagacc tcgagccgca aaaggtgcgt gtgcgagtcc
      721 acgagcatag cgcccatcac ctcttcccca gagccatcag cgaggggcat tataaaggcg
      781 ccgggtacct tcttcagccc cttaccagcc gctcgtagat ctcactgcga cgatgtaccg
      841 actcgcgggc ggccttctga gctgcccgcg aagggctctc cccctccgac atgtatcctc
      901 gagctcgcct gattgcctcc tctatgctct cttccgtctt cgccgttccg ccgcgaacca
      961 cgagcacgat ctcacccttt gctccccccg agaaataatt tgcagcctcc tcagccgtcc
     1021 ccctgaagac ctcctcgtaa cgcttcgtaa gctccctgca cacggcgacc ggggtccggg
     1081 caggaaggtc tcccagtgtc ttcccaagcc ggtgcggtga ctcgaagagc acgaaggtag
     1141 aaggctcggt ggcgacgagc tccaatagct ccgtacggcc ccttcccttg cgaggcggga
     1201 agccggtgaa gacgaccgtg tcggcgggta gccccgagac cacgagggcc gtggtgaggg
     1261 ccgatgggcc agggagggct tctatctcga cgccggcctc gatgcaggcg cgcactagcc
     1321 ggtgtcccgg gtcggagacg aggggggtgc ctgcgtcggt gacgagggca acgcgctctg
     1381 tctgcgccct ttcggccagt tcgggggcca ggcgttcctc gttgtgctcg tggtaggcga
     1441 gcaggttctt cttcatgtca tagtgggcga gcaggcgtcc tgtgcgcctg gtgtcctcgc
     1501 aagctacgag gtcggcctcg cgcaagtagc ggagggcgcg gagggtgatg tcctccagat
     1561 tgcctatggg ggttgggatc actgtgaggg gcatctctca gccgccgagc acgtactctc
     1621 cggacgatgc gtcccgggcg agcgccgcgg cgccgaggac gccggatctg ggaccgaggg
     1681 tagcgacttt gacgtcagcc aggtcgcgtg aaggaggcct ggcgcggagg atgacttctt
     1741 tgcgggctgc tcttagtacg agctccccgg cctccatgac ccctccgccg accgcgacca
     1801 cctcggggtt gaagatgttc acgaatccgg caaggccaac gccgagccat ttccctgcct
     1861 cgtcaagtac cgagagcgcc acctcgtccc cttcgcgggc gagctccgtt acgtcctcgc
     1921 ccagtatctg gcgcctcgtg gccaggcgtc ccagcgccga accgggttgc tcgaccgcaa
     1981 gctcgcgggc gcggcgtcct attgcggtgc cagaggcgag ggcctccagg caaccgtggt
     2041 tgccgcaggc gcagcgtggc cctgtggcct ggatggttac gtgcccgagc tcgccgcccg
     2101 agccctgcgc gccgcgcatc aggatgccgt gcgtgatgac gccgcccccg atccctgttc
     2161 ctagcgcgac gaagaccagg tggtcggcag cgaggccgac c
//
LOCUS       DASTUV010000312         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_654885, whole genome shotgun sequence.
ACCESSION   DASTUV010000312 DASTUV010000000
VERSION     DASTUV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654885"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="VFT03_06265"
     CDS             complement(<1..1852)
                     /locus_tag="VFT03_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEU4847783.1"
                     /translation="MTDTTAASKGPADRLKRWLLAEVVEDVPGPQAKEGRSEQHPWWQ
                     VVCLTGVDYFSTLGYIPGIAALAAGVLSPIATLFIVLLTLFGALPMYRRVAEQSPHGQ
                     GSIAMLERLLSFWKGKLLVLVLLGFVATAWLVTITLSSADAAVHIAENPLVPGFLHDQ
                     EVLITLVLLVVLGAIFLKGFKEAIGVAVMIVGVYLLLNVVVVAVGFYEITMHPRSVID
                     WQAALFSTYGNPLLMIVASLLVFPRLALGLSGFETGVGMMPLVRGDPDDDPERPAERI
                     RNTKKMLTIAALIMSFYLVTTSLVTTVLIPAREFEPGGSANGRALAYLAHYYLGDAFG
                     TAYDLSTIAILAFAGASALAGLLNIVPRYLPRYGMAPEWARAIRPLVLVYIAISIIVT
                     IIFRADVDAQAGAYATGVLAMMSSAAFAVTLSARSRGSRTWMLVFGVVTVVFVYALVA
                     NEIQRPDGLVISSFFIVTIIFTSLVSRVYRSLELRQERIELAETVRRFVDEASHEGEI
                     HIVANRRQAGDEREYAEKEKEQREDNHIPEHFPILFLEIDIEDPSEFEDVLEVNGVEV
                     SGHKVLRAKSSVVPNAIAALLLHLRDTTGRTPHCYFGWTEGNPIIYLLRFL"
     regulatory      complement(1923..2073)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(2290..>2576)
                     /locus_tag="VFT03_06270"
     CDS             complement(2290..>2576)
                     /locus_tag="VFT03_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEU4847784.1"
                     /translation="TVLTLATLLVLVGTLALMVSDELSLLPALFEVTSAFGTAGLSLG
                     ITPELSAFAKVLVAAVMLVGRVGPLILLLALSSRQRPINFKYPEEGIALG"
BASE COUNT          518 a          797 c          802 g          459 t
ORIGIN      
        1 gaagaaaacg caacaggtat atgatggggt ttccttcggt ccagccgaag tagcagtggg
       61 gcgtcctgcc tgtggtgtcg cgcaggtgca gcaacagtgc tgctatggcg tttggcacca
      121 ccgagctctt ggcgcgcaac accttgtgcc cgcttacctc cacgccgttg acctccagca
      181 cgtcctcgaa ctccgagggg tcctcgatat cgatctcgag gaagaggata gggaagtgtt
      241 cgggtatgtg gttgtcttcg cgctgctcct tttccttttc agcgtattct cgctcatcgc
      301 ccgcctggcg ccgattggcg acgatgtgga tctctccttc gtggctagct tcgtcgacga
      361 atcgtcggac cgtttcggcc agttcgatcc gctcctgcct gagctcgagc gagcggtaca
      421 cccgcgagac gagagaggtg aagattatcg tcacgatgaa gaacgaggag attacgagcc
      481 catcgggacg ctggatctcg ttcgcgacga gagcgtagac gaacacgacc gtgaccaccc
      541 cgaacaccag catccatgtc ctcgagcccc tgctccgggc ggagagcgta acggcaaagg
      601 cggccgagct catcattgcc aacacgcctg tggcgtaagc tccggcctgg gcgtccacgt
      661 cggcccggaa gatgatagtc acgattatag agatggcgat gtagaccagg actagcggcc
      721 tgatcgcccg cgcccattcg ggagccatgc cgtaacgggg caggtatcgt ggcacgatgt
      781 tgagcagccc ggccagagcg gatgctcccg cgaaggccag gatcgcgatc gtggagagat
      841 cgtaggccgt cccgaacgcg tcccccaggt aatagtgcgc caggtaagcc agcgctctcc
      901 cattggcact accgcccggc tcgaactccc gggcggggat gagcacggtc gtcaccaggc
      961 tcgtcgtgac caggtaaaag ctcatgatga gagcggcgat ggtcagcatc tttttcgtgt
     1021 tgcggatacg ctctgcgggg cgctccgggt cgtcgtcagg gtcgccgcgc acgagcggca
     1081 tcatgcccac gccggtctcg aagcccgaga gccctaaagc cagacgagga aacaccagca
     1141 gtgaggcgac gatcatcagc aaggggttgc cgtaggtgga gaacagtgcg gcctgccaat
     1201 ctatgacgct ccgggggtgc atcgtgattt cgtagaagcc gacggccacc acgacgacgt
     1261 tcaacaacag gtagactccg acgatcatga cggcgacgcc gatggcttcc ttgaaaccct
     1321 tgaggaagat ggcgcccagc acgaccagga ggaccagcgt gataaggacc tcctggtcgt
     1381 gtaagaaacc tggcaccagc ggattctctg caatgtggac cgcggcgtca gctgaggaga
     1441 gcgtgatcgt aaccaaccag gccgtcgcca cgaatcccag aagcaccagc acgagaagct
     1501 tgcccttcca gaaggagagc agcctctcta gcatcgcgat cgagccctgc ccgtgcgggc
     1561 tctgctcggc gacccgcctg tacataggca aggccccgaa gagggtcaga agcacgatga
     1621 aaagggtcgc tatgggcgag agaacaccgg cggcgagcgc ggcgatgcct gggatgtacc
     1681 cgagggtaga gaagtagtca acgcctgtca ggcacactac ctgccaccag gggtgctgct
     1741 ctgaccttcc ctcttttgcc tgaggacccg gcacgtcctc gacgacctct gcgagcaacc
     1801 accgcttcaa tcggtcggca gggcccttcg atgccgccgt cgtgtcggtc accacgattc
     1861 tctcgccggg ggccagtgac cggcggataa acgcgcccgc aaggaccgcc gctcggacat
     1921 tggatctcct tctgttgcgc ctgcggggtt agctgtcggg ttcgggccga aagagtcaag
     1981 ccctacaccg gcggaccggc gattcacccc tggcggtctc taccaccgtt tgggtccccc
     2041 gctctcctcc cggagactcg gcgtcgcggc atttttctgt cactcgacgg tatacgcccc
     2101 ggacagctgg ttgtcaacgg tcgcggacgc tagcacgttc accgaagtcg gcctgtaggg
     2161 tcttctatga ggtccacttc caggaagccg tatagagccg tctccgtgaa ccctctgtcc
     2221 ccggtactca ccagacgaac cccgcgcgcg cctggtggcc cctagtccga gcgtacttcg
     2281 aagacgttac tagccaaggg ctatgccctc ctcgggatac ttgaaattga ttggtctctg
     2341 cctggacgag agggccagga gcagaattag tggacccacg cgcccgacca gcatgactgc
     2401 cgctacaagg accttggcga acgcggagag ctccggcgtg atgccaagcg ataagccggc
     2461 tgtgccgaag gccgaggtga cctcgaagag ggccggtagc agagacaact cgtcggagac
     2521 catgagcgcg agcgtgccga ccaacactag tagcgtcgct agcgtcagca cggtaa
//
LOCUS       DASTUV010000313         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_170732, whole genome shotgun sequence.
ACCESSION   DASTUV010000313 DASTUV010000000
VERSION     DASTUV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170732"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFT03_06275"
     CDS             complement(<1..513)
                     /locus_tag="VFT03_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847785.1"
                     /translation="MVCGPTASGKSEVSDALADALTREHGAIVTTLVVDSMQVYREIP
                     TITNQARRRPAELLGIVSVTEEWTVARHRALAEEIIAQNTTPFVLDAGTGMYLNAILL
                     DIPLAPKVEPGTRKLAQSLSSGATNPRRAARARELELAGAAPRRSIWDGEPRYDTSVI
                     YLRPDKEIHDA"
     gene            complement(545..1903)
                     /locus_tag="VFT03_06280"
     CDS             complement(545..1903)
                     /locus_tag="VFT03_06280"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEU4847786.1"
                     /translation="MSDATATPPTATRVTISRKPGARGARAAPSRSEGTRTACIRTFG
                     CQMNVHDSDRMRRMILDAGYAEVHSYEEADLVILNTCYVRENAVDRVRGHLGELNRLR
                     REGRVKKVALTGCIGASDESTALQRQYNIDLVLGTHNTYELAEFVGLPTMRETYTPDL
                     PGTEGEHSAFVTIMTGCNYQCSYCIVPSVRGRMVCRPLENVVAEVEGLVESGTGYVTL
                     LGQTVDAWRHEGKRFCDLLERVAEVAPRVWFTTSHPSNMEDRTLRLIGEKATIVKRLH
                     LPVQSGSDRMLKVMHRGYKADRYRRKIEVFREAVPDGTLSTDIIVGHPGETEEDHEQT
                     LELVEDCAFDSAYIFKFSPRKGTEAAELGGTVPPEVVQRRFLEVLRAVEQNAFSRNQR
                     KVGSLEDVYIRHGSTEDGKAIGETWSGHAVHVRTDREPGEYVRARIESAGPHVLYAGE
                     PG"
     gene            complement(1896..2234)
                     /locus_tag="VFT03_06285"
     CDS             complement(1896..2234)
                     /locus_tag="VFT03_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847787.1"
                     /translation="MGLGLELSLRTVQRVWRGEVRPAGAVSPARAGDDAGRKKGDRRR
                     IEEGPIEDSPIEEGPVLVVGEEERGAVADILRRAARASFLEVGELQLESRPGEISVRV
                     RVYEPEENYE"
BASE COUNT          336 a          810 c          694 g          477 t
ORIGIN      
        1 ggcgtcgtgt atctctttgt ccggtcgcag gtagatgacg gaggtgtcgt agcggggttc
       61 tccgtcccag atcgaacgcc gtggcgcggc gccggcgagc tcgagctccc tggcccgtgc
      121 cgcgcggcgc gggttggtgg cgccgctact caagctctgg gcgagctttc tcgtgccagg
      181 ctccaccttc ggcgcgaggg gaatgtcgag caatatcgcg ttcaggtaca ttccggtccc
      241 cgcgtccagg acgaacggtg tggtgttctg cgctatgatc tcctccgcaa gcgccctgtg
      301 gcgggcgacg gtccattctt ccgtgacgga gacgatgccg agaagttcgg cgggccggcg
      361 gcgcgcctgg ttggttatcg tagggatctc gcggtacacc tgcatcgagt cgacgacgag
      421 ggtggtaacg atcgccccgt gctcccgcgt gagcgcatcg gcgagggcgt cggagacctc
      481 gctcttcccg ctcgccgtcg gcccgcagac caccagggcc tttttgtttt gctccgccgt
      541 caggctaccc cggctccccg gcgtacagga cgtgcggccc ggcagactct atcctggccc
      601 tgacgtactc tccgggctct ctgtccgtcc tcacgtgcac cgcgtggccg ctccaggtct
      661 cgcctatggc cttgccgtcc tcggtgctcc cgtgccggat gtacacatct tccaggcttc
      721 ccaccttgcg ctggttgcgc gagaaggcgt tctgctcgac cgctcttagc acctccagga
      781 agcgccgctg cacaacctcc ggcggcaccg tgcctccgag ctctgcggcc tccgtgccct
      841 ttctcggaga gaacttgaaa atgtaggctg aatcgaatgc gcagtcctcg accagctcga
      901 gcgtctgctc gtggtcctct tcggtctctc ccgggtggcc gactatgata tcggtgctga
      961 gggtcccgtc cggaacagcc tccctgaaca cttcgatctt gcgcctgtac ctatccgctt
     1021 tgtagccccg gtgcatgacc ttgagcatgc ggtccgagcc ggattgcacg ggcaagtgca
     1081 gcctcttgac tatcgtggcc ttctccccga tcaaacgtag cgtccggtct tccatgttgg
     1141 aagggtggct ggtcgtgaac catacccgcg gagccacctc cgcgaccctc tccagcaggt
     1201 cgcagaaccg cttcccctca tgcctccacg catccaccgt ctgcccgagc aaagtgacgt
     1261 aaccggtccc gctctccacg agaccctcga cctccgcgac gacgttctcc agcggcctac
     1321 acaccatccg ccccctcacc gacggcacga tgcagtagct gcactggtag ttgcaccccg
     1381 tcatgatggt gacgaacgcg ctgtgctcgc cttccgtccc cggtagatcc ggcgtgtatg
     1441 tctcccgcat cgtcggcaac ccgacgaact ccgcaagctc gtacgtatta tgggtgccca
     1501 ggacgagatc tatgttgtac tggcgctgga gggcggtgga ttcgtccgag gcgccgatgc
     1561 agccggtgag ggcgactttc ttgacgcggc cctcgcgcct gaggcggttg agctcgccga
     1621 gatgaccgcg gacacggtcg acggcgttct cgcgcacgta gcaggtgttg aggatgacga
     1681 ggtcggcctc ctcgtaggag tggacctcgg cgtagccggc gtccaggatc atgcgccgca
     1741 tcctgtccga gtcgtggacg ttcatctggc agccaaaggt tcggatgcag gcggtcctcg
     1801 tcccctcgga cctggatggc gccgccctgg caccgcgcgc tcctggcttg cgcgagatcg
     1861 taacccgggt ggcggtcggc ggcgtcgctg tggcgtcact catagttttc ctccggctcg
     1921 tagacgcgga ctctcacgga gatctctccg ggacggctct cgagctggag ctcccctacc
     1981 tcgaggaaac tcgccctcgc cgcccgccta agtatatccg cgacggcgcc cctttcctcc
     2041 tcccccacca caagcaccgg accttcttcg atcggactat cttcgattgg accttcttca
     2101 atcctccgcc ggtccccctt cttcctgcca gcgtcgtcgc ccgctctggc aggagatacg
     2161 gctcccgcag gcctcacctc cccccgccat accctctgca ccgtgcgcag cgataactcg
     2221 agccccagac ccaacgccct cctccttagt tccgccgttg tctcgcatga atgtattatg
     2281 cggttcttta ggagggtagc gaggatacgg gcgggag
//
LOCUS       DASTUV010000314         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1258665, whole genome shotgun sequence.
ACCESSION   DASTUV010000314 DASTUV010000000
VERSION     DASTUV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258665"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..456
                     /locus_tag="VFT03_06290"
     CDS             169..456
                     /locus_tag="VFT03_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU4847788.1"
                     /translation="MSEAASGGRKLLIVGAHSGDFVWRAAGAIAAATSRGGEARVVAL
                     SYGERGESGDLWNEPDQTIEYVKRARYATGEAGVRYAEAFQRTIPQVVRSL"
     gene            453..1094
                     /locus_tag="VFT03_06295"
     CDS             453..1094
                     /locus_tag="VFT03_06295"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEU4847789.1"
                     /translation="MKGEEAGVVVGLRDLGVATVYEASGRRGLVDAPLERVVPGSRAA
                     GPARTVLCGQGDNLAVHAALARVQPGEVLVLTMPEPEPVAIVGEVIAVQAKVRGAAGL
                     LIDGAIRDADELSELGLPVWARFVHARGAAKVEAGELDVPVTVGSVRIKPGDVVVMDG
                     DGAVVVPAGCAEEVLEAGRRREANEAAMMDELRAGALTLDLMGLREKLGADGG"
     gene            1564..1911
                     /locus_tag="VFT03_06300"
     CDS             1564..1911
                     /locus_tag="VFT03_06300"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU4847790.1"
                     /translation="MSELSEMRCTACRGDEPTLTDAEIAEMRPQVPEWQVVQQEGINR
                     LERVFRFRNFAEALAFANRVGEQAEEEGHHPALLTEWGKVTVTWWTHKIGGLHRNDFI
                     MAARTDELSEGRL"
     gene            2109..>2430
                     /locus_tag="VFT03_06305"
     CDS             2109..>2430
                     /locus_tag="VFT03_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU4847791.1"
                     /translation="MEFGLYTFADVGPENDQARRLHNLLEEIELADQLGLDVFGVGEH
                     HRPDYAVSSPAVTLAAAAARTKDIRLTSAVTVLSSDDPVRVFQDFATLDLISNGRAEI
                     MAGRG"
BASE COUNT          465 a          680 c          888 g          397 t
ORIGIN      
        1 gattacgttc gtgctgtgga cgaacggcgg aggacagcct gatgggcggg tgcttatgta
       61 ctttttcaac cgagtgcctg gtactagtat taggtgcata ggggcataat cacgtgcgaa
      121 cgatgctcaa ggagctctag ggacggacga gaaggaaggt gtgggcatat gagcgaggcc
      181 gctagcggcg gcaggaagct gctgatcgtg ggggcacact caggcgactt cgtgtggcgg
      241 gccgcgggcg cgatcgcggc cgcgacctcg cgaggtgggg aggcacgggt ggtggcgctc
      301 tcctacggcg agcgcggcga gtccggggac ctttggaacg agccggacca gaccatcgag
      361 tacgtgaaga gggccaggta cgccaccggc gaggccggtg tgcgctacgc ggaggccttc
      421 cagcgcacga tcccgcaggt ggtgaggtcc ctgtgaaggg ggaggaagcg ggcgtggtcg
      481 tcgggctccg tgacctcggg gtggcgaccg tctacgaggc gtcggggcga agggggctcg
      541 tcgacgcgcc cctcgagcgc gtggtaccgg ggagcagggc cgcggggccg gcccgcaccg
      601 tcctgtgcgg ccagggggac aatctcgcgg tgcacgccgc gctggcgcgc gtccagccgg
      661 gagaggtgct ggtgctgaca atgcccgagc ccgaacccgt ggccattgtt ggagaggtca
      721 tcgcggtcca ggcgaaggtg cgcggggcgg ccggcctgct gatcgacgga gccatccggg
      781 acgcagatga actgagcgag ctggggctcc cggtctgggc caggttcgtc cacgcccgcg
      841 gggctgccaa agtggaagcg ggtgagctcg acgtgcccgt gaccgtgggc agtgtgcgga
      901 taaagcccgg cgacgtggtc gtgatggacg gggacggcgc ggtcgtggtg ccggcgggat
      961 gcgcggagga ggtgctcgag gccggtcgga ggcgggaggc taacgaagcg gcgatgatgg
     1021 atgagctgcg tgcgggggcg ctcaccctgg acctgatggg cctgagggag aagctggggg
     1081 ccgatggtgg ctagggctac gacagaagcg gaacaggtaa gatgcggcac ctcgagcgcc
     1141 gctcccatgg accgctcctc agctgcagcg aaacgttaga acacagccgc accaggaccg
     1201 gcctcggtcg gggaatagag gttggctcgt cgggtgatag atgctgggat ctcgatgagc
     1261 atagtgattt acggcagcct tacatcgtcg gtgctaaatc ctgtgcagca accgtgcggc
     1321 aacacggata gagtagtaag ggcgcagccg gtgtatggga aggaggcggg gagaccgtct
     1381 gggccgtgag cagctaaagc gctcgcatcc ttctcagaga tcatgggcag gtccggccgg
     1441 ctgaggctca tggggatcta atagcctgct actgagtaga tttggaccgc tcgagccgct
     1501 gaggagtcta tccggaggat agacttagca cctaacctac aagctcacag aaaggaggaa
     1561 gacatgagcg agctgtcgga gatgaggtgc accgcctgcc ggggcgacga acctacgcta
     1621 accgacgccg aaattgcgga gatgcgcccg caggtccccg agtggcaagt agtgcagcag
     1681 gaaggcatca atcgcctaga gcgcgtgttc aggttccgta actttgccga ggcactggct
     1741 ttcgccaaca gggtaggcga acaggccgag gaggaggggc accaccctgc actgttgacc
     1801 gagtggggca aggtaacggt cacctggtgg acgcacaaga tcggtggtct gcaccgcaac
     1861 gacttcataa tggcggcgag gacggacgag ctctccgaag gacggttgta gccacgtcta
     1921 tatcgtagag gagctcctac cttttcgcga gctcgaggac caagataata tttgatcagc
     1981 tcttggtcac ctgacagctt accgagtcgg cgcagtggta tgcgatcgct gtgggcgccg
     2041 cctgcttcaa gcgcttcgaa caagctcaga cctacgtcac gacggactcg gcccgaaagg
     2101 aacccaagat ggaatttggc ctctacacct tcgccgacgt cggccccgaa aacgaccagg
     2161 cacggcggct gcacaacctc ctcgaggaga tcgagttggc ggatcagctc ggcctcgacg
     2221 tcttcggagt cggtgagcac catcgtcccg actacgccgt ctcatcgccg gccgtgacgt
     2281 tggcggcggc cgcagcccgt acaaaggaca ttcgccttac gagcgcggtc accgttctga
     2341 gctccgacga tcccgtgcgc gtcttccaag acttcgccac gctcgatctc atctcgaacg
     2401 gtcgcgcgga gatcatggcc ggccgcggct
//
LOCUS       DASTUV010000315         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_507050, whole genome shotgun sequence.
ACCESSION   DASTUV010000315 DASTUV010000000
VERSION     DASTUV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507050"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFT03_06310"
     CDS             complement(<1..257)
                     /locus_tag="VFT03_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847792.1"
                     /translation="MVTTEAKVRENGKANGAREVDTICGFCGVGCGLTLKVENGVIQK
                     VTGEKANPSSKGEACIKGREGWRHLYSDRRLTTPLIRKDGEL"
     gene            complement(483..1334)
                     /gene="fdhD"
                     /locus_tag="VFT03_06315"
     CDS             complement(483..1334)
                     /gene="fdhD"
                     /locus_tag="VFT03_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU4847793.1"
                     /translation="MLKNTSRRTSKTKVRVRVVEDGRARVRPDSLATEEPMEIRLIAG
                     GQRQTVAVTMRTPGADFELAAGFLYGEGIVSSAEDIKKISYCVDPDVDAEQQYNIVNV
                     ELRGGRDYDLRPLERHFYTSSACGVCGKASLEQLELRGCPVVPPGPRIPAEQIYALPE
                     KLREAQGLFEATGGLHAAALFDAEGELVALREDVGRHNATDKLVGWALLERRLPLSDH
                     IVMVSGRSSFEILQKSLIAGAPIVCAISAPSSLAVDVARQFGMTLIGFLRGERFNVYA
                     GEGRIAL"
     gene            1402..1629
                     /locus_tag="VFT03_06320"
     CDS             1402..1629
                     /locus_tag="VFT03_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847794.1"
                     /translation="MLRLMGLPRVERFPEATVTREDEKLVIHFGGLGLEQVMSVPLKY
                     VEGDEEAAELRLLAQLQEIGYRVERVPPQVG"
     gene            complement(1617..>2284)
                     /locus_tag="VFT03_06325"
     CDS             complement(1617..>2284)
                     /locus_tag="VFT03_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847795.1"
                     /translation="FSESDDIFADMKTMCDQYRSGSYMSWQCYHGIGHGLMFYTANDL
                     PRSLKMCEGFDTDFGRSNCANGVFMENFNADQKLHISKFLKESDPFYPCPQQENRYKS
                     HCYLYAPTYFLSLNGSDYDAALEWCNGAEAGFEPSCAYGVGTQAMKENLNDPKLVESV
                     CMNGEDGQTAPCIEGMTSLYINHHGSLEPARELCSKLEKPNRPACYDAIAANASLFRD
                     QPT"
BASE COUNT          369 a          746 c          690 g          479 t
ORIGIN      
        1 agttcgccgt ccttgcggat gaggggcgta gtcagtctcc tgtcgctgta gaggtggcgc
       61 cagccctcgc ggcctttgat gcacgcctcg cccttgctcg acgggtttgc cttctcgccc
      121 gttaccttct ggatgacccc gttctcgacc ttgagggtca gcccgcaacc cacgccgcag
      181 aagccgcaga tcgtgtctac ttcccgcgcg ccattggcct tgccgttctc tctgaccttc
      241 gcctccgtcg taaccatcgc cctcgcctca cctcttgttg taacgaagtt tctttgatcc
      301 ccattctagc ctcgatggcc ggacttgccc accattggcg tccgaaagga accttgagcg
      361 atctgttaac acaagcttgg catcacatcg cctcgccgct ctccagcatg ttggcgcggg
      421 tagacgcctc gcagagcatt gccgcgcacc gaggtccgga actgctgcct gaaacctgag
      481 gcctagagcg ctatccgacc ttctccggca tagacgttga accgctctcc gcgcaggaaa
      541 ccgatcaggg tcatcccgaa ctggcgcgcg acgtcaacgg caaggctgct cggggctgag
      601 atggcgcaca cgatgggggc tccggcgatc agggatttct gcaggatctc gaagctgctg
      661 cgtccgctga ccatgacgat gtggtcgctc aggggcaacc gccgctccag tagtgcccag
      721 cccacgagct tgtctgtggc gttgtggcgc cccacgtctt ccctgagcgc cacgagctcg
      781 ccctccgcgt cgaagagcgc cgccgcgtgc agcccgccag tcgcctcgaa gagcccttga
      841 gcctcgcgca gcttctcggg gagggcgtag atctgctccg ccggtatccg tggccctggc
      901 gggaccacgg gacacccgcg tagctcgagc tgctccaggc tcgccttgcc gcaaacgccg
      961 caggcgctcg aggtgtagaa gtgtcgttcg aggggtctca ggtcgtagtc ccgcccgccc
     1021 cgcaattcga cgttgacgat gttgtattgc tgctcggcgt ccacgtccgg gtccacgcag
     1081 tagctgatct tctttatgtc ctcggccgaa gagacgatgc cctcaccgta gagaaacccg
     1141 gcggcgagct cgaagtccgc acccggcgtc cgcatggtga ctgccacggt ctgccgctgg
     1201 ccgccggcga tcaggcgtat ctccatcggc tcctcagtcg ccagggagtc gggccggacg
     1261 cgcgccctgc cgtcttcgac gacccgcacc cggaccttcg tcttgctcgt gcgcctcgaa
     1321 gtgttcttca gcactctgtt ctccgctcac gctcggaccc tgatgttaag tctagcaccg
     1381 cgccgtgcta gcatattgcc catgctaagg ctcatgggtc tgccgcgtgt agaacgcttc
     1441 ccggaggcga ccgtcaccag ggaggacgag aagctcgtga tccacttcgg cggtctcggg
     1501 ctcgagcagg tcatgagcgt acccctgaag tacgtcgagg gggacgagga agcggccgag
     1561 ctccggctgc tggcgcagct gcaggagatc gggtacaggg tagagcgagt cccgcctcag
     1621 gtgggttgat ccctgaacag gctggcgttc gccgctatcg cgtcgtagca ggctggccgg
     1681 ttcggcttct ccaacttgct acacagctct ctggccggct ccagtgagcc gtgatggttt
     1741 atgtacaggc tcgtcatccc ttcgatgcag ggcgccgtct gtccgtcttc gccgttcatg
     1801 cacacggact cgacgagttt ggggtcgttc aggttctctt tcatggcctg cgtccccacc
     1861 ccgtaagcgc acgacggctc gaaaccggct tccgcgccgt tgcaccactc gagagcagcg
     1921 tcgtaatcgc ttccgttgag gctgagaaag taggtcggag cgtaaaggta gcagtgagat
     1981 ttgtagcggt tctcctgctg cgggcaggga tagaaggggt cgctctcctt caggaacttc
     2041 gagatgtgca gcttctggtc ggcgttgaag ttctccatga acactccgtt ggcgcagttg
     2101 gatctaccga agtcggtgtc gaaaccctcg cacattttca gcgaacgggg caggtcgttc
     2161 gcggtgtaga acatcaggcc gtgcccgatg ccgtggtaac actgccagct catatagctc
     2221 cccgacctgt actggtcgca catcgtcttc atgtccgcga agatgtcgtc gctctcggag
     2281 aaac
//
LOCUS       DASTUV010000316         6328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1252859, whole genome shotgun sequence.
ACCESSION   DASTUV010000316 DASTUV010000000
VERSION     DASTUV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252859"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1646
                     /locus_tag="VFT03_06330"
     CDS             222..1646
                     /locus_tag="VFT03_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEU4847796.1"
                     /translation="MRFERALPGTNEVFLADGRPRRIYDLVLDELRRTGPGKWEEKLR
                     RAHGLLLEEQYSFGILEGDKTHPTDWIPRIVPASDWERLVRGLEQRMRAVNEFLRRLE
                     AGKEEVVPREVIESSILYDVSTPNRFGPVPVRQIAFDVVAVESRRAGETGGWEYLLIE
                     ENAKMPVGLAAMTRRRLMSREVFFPESYAEMPVHSLDGWLGRLGDALRAASPKGGGAT
                     LAVVSGGPADQFYLDHHIYAREMDAVLAETRDLSIDGEGFLVHGPTGRRIDVIYERVD
                     EDRLYAEVPGLLEAHVEGKVHVLFAPNSEIVDDKGVEAFVPEMVRTYLGEEPLIQNAR
                     TWSLSVEEDYRYVMNHFGDLVVKSRGGYGGKEVMIGPEETEESVERFRKMVEENPVEY
                     VAQELIDFSTHVLCETGEEGFLLRDSYADYRMLVLAPDPEDPNVVETVPGSLSRVAAP
                     GKHIVNISSGGKMKDTWVLGGQGR"
     gene            1643..2569
                     /locus_tag="VFT03_06335"
     CDS             1643..2569
                     /locus_tag="VFT03_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847797.1"
                     /translation="MNYGEIITSAFRITLYNRYLWFFGFFVGLGSGGGGGGGGGDFDE
                     QPSAEIASTAAVQQGAFDNALLIVGLVLVGLLLLLVFFALYVVSQGGLTESVAAIDRG
                     EERRFSSTWRAGARNFWPVLWQVLLFVGIVLGLLLAVGIPVALLVAGAFATGSTALRA
                     AAVFVAVLVAIALLVVVFVLLAIVRQFARRELVLGGIDVVGSVGRGYRLLRRNLGRSL
                     LVWLIQLGLMLAAAIALLIALVIVGLLLFLPTILLAVAQYTTAAIVAGVVAGLILLPL
                     LIAALAVLGTFNHSYWTLAYPRLTNQGGTTRP"
     gene            2566..3513
                     /locus_tag="VFT03_06340"
     CDS             2566..3513
                     /locus_tag="VFT03_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847798.1"
                     /translation="MNYGDLLSEAFRLAWRNRFLWFFGFFVAGGGGSFNFPANFSGQE
                     VQNPLGPVAGPLQWISDNLVLFLTVVISVIVVLALIFLVLSMISQGALAESVAALHRG
                     ETRSFGSAFRVGTANFWRVFGLKVLFFLIALGLVLVIFLPVVLGALAAFSLTDSTGLR
                     VLAVVLGVLFVFVALIVVFLPFAIVNQFGLRELVVSRRRITESIAGGFRLFRRNIGRS
                     LLVWLIQLAVMLGLGIALLVALVIFGAILLGPAIALFATDHANAGVVAGVLGGVLFLV
                     PVFVISGAIGAFNHAYWTLAYLRLVEPPEDLEPAQASAA"
     gene            3544..4290
                     /locus_tag="VFT03_06345"
     CDS             3544..4290
                     /locus_tag="VFT03_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4847799.1"
                     /translation="MAKLDGKVAVVTGASSGIGEATAEALAREGAAVVVAARREDRLA
                     GLVERIEGAGGRVLAAVCDVTDESQAHALIRKAVEEFGSVDILVNNAGVMLLSTVGKS
                     LSDQWRQMFEVNVIGLLYTTDAAIGHMKEQGSGHLVNISSVAGRKVTRASSGVYAGSK
                     HAVVAISEGLRQELLDDNIRVTVVEPGAVATELTDHITDEDAREAVSGLHELDILHSE
                     DIAEAIVYAVTQPDRVSVNEVLIRPTQQPV"
     gene            complement(4343..5164)
                     /locus_tag="VFT03_06350"
     CDS             complement(4343..5164)
                     /locus_tag="VFT03_06350"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEU4847800.1"
                     /translation="MAKDAAEPDRKERLVFGGMAHWDGLDLFGPNYMSVAYRRKGEIH
                     VRVEPSKIWKPKNPTVERISRWPIIRSLFFWCRLLLQVVGSFWVLVFFGASLAGLWLF
                     VRLMELGSGAGPLGGVFAFLAEFPILPVLLLFFAAMKFTSIGRYHGAEHKAVAAYEEY
                     GEVTLEGARKSSRVHPRCGTNILGYIVLAAVLDPLVGWWGYAILQFILISEAWFVLGK
                     TRPSISVGNFLQRYFTTTEPGRAELEVAVQSLNKLLQAEEERAAGGDLVQAPARS"
     gene            5247..>6328
                     /locus_tag="VFT03_06355"
     CDS             5247..>6328
                     /locus_tag="VFT03_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4847801.1"
                     /translation="MAEDDQAPPGDFVKGSSDAARKRIIYVSIGLIFALLVASYLVYQ
                     IAVVVLVLLLTMLFSVIISAPVDYLENSGMSRGLGTLVVLGGLVLGIWIAGVALAPTI
                     ADQAVQFWETVPELLENAQDHAGQLREALGLGSSFGLGSLNVVDSARNFLSGGALTTV
                     ANVGAGVASVISYLVVILIATIYSVAQPGPLVNGFVALFPAGRRQEVRRILRELYETI
                     QRWFVGQLASMLLIGVLSTVALYLIGVPFALLLGIFSGLISFIPFVGPLISVIPPLLL
                     ALIGTPIDALWVVLAYAAIQTIESYLIQPLVMSRAVSLHPAVVMFALLIMGTLFGFVG
                     VLIAVPLVAAIAVLLRELWIERMDSLG"
BASE COUNT         1011 a         1985 c         2125 g         1207 t
ORIGIN      
        1 agccttcggc ttccgcttct cagctacgct tcggcccttc gggcctcgct ttcagcacgc
       61 cctcttcgag ggcttgtcag cttctctcag cacttgctag ctgaaatgct gacaagctgc
      121 ctgaaaggca gcgtgctgac aagcggaggc cgcaaggccg gagcgtgctg aaaagccgcc
      181 gaccgtaggg aggaggcgtg ctcctatata attccttgct tgtgaggttc gagcgcgcac
      241 tccctggcac caacgaggtc tttctagctg acgggaggcc gcgccggatc tacgatctcg
      301 tcctcgacga gttgaggcgc acgggtcccg ggaagtggga ggagaagctg cgccgggcgc
      361 acgggttgct gctggaggag cagtattcct tcggaatatt ggaaggtgac aagacccatc
      421 cgacggattg gatcccccgc atcgtcccgg cttcggactg ggagcgcctg gtgcgcggcc
      481 tcgaacagcg gatgcgcgcc gtgaacgagt tcttgaggag gcttgaggcg ggcaaggagg
      541 aggtagtccc gcgggaggtt atcgagtcga gcatcctcta cgacgtctcg acccccaacc
      601 gcttcggccc cgtgccggtg cgccagatcg ccttcgacgt cgtggctgtc gagagcaggc
      661 gggcgggcga gaccggcggc tgggagtacc tcctcatcga ggagaacgcc aagatgccgg
      721 tagggctcgc cgccatgacc cgccggcggc tgatgagccg cgaggtgttc ttcccggaat
      781 cctacgctga gatgcctgtc cactcgctag acggctggct cgggcgcctg ggcgatgcgc
      841 tgcgagccgc ctcccctaaa ggtggcggcg cgacgctcgc cgtcgtcagc ggcggtcccg
      901 ccgaccagtt ctacctggac caccacatct acgccagaga gatggacgcc gtccttgccg
      961 agaccagaga tttgagcata gacggcgagg ggttcctggt gcacgggccg accgggcgca
     1021 ggatagacgt gatctacgag cgcgtggacg aggacaggct ctacgccgag gtgcccggtc
     1081 tcttggaggc ccacgtcgag gggaaggtgc acgtcctctt cgctccgaac tccgagatcg
     1141 tggacgacaa aggcgttgag gccttcgtcc ccgagatggt gaggacatac ctcggggagg
     1201 agccgctcat ccagaacgcc aggacctggt cgctctcggt cgaggaagac tacaggtacg
     1261 tcatgaacca cttcggggac ctggtcgtca agagccgggg cggctacggg ggcaaggagg
     1321 tcatgatcgg gccggaggag acagaggagt ccgtagagag gttccggaag atggtggagg
     1381 agaaccctgt cgagtacgtg gcgcaggagc tgatagattt ctctacccac gtgctctgcg
     1441 aaaccggcga agaaggcttc ctccttcgcg actcctacgc cgactaccgg atgctcgtcc
     1501 tcgctcccga ccccgaagat cccaacgtcg tcgagacggt ccccggctcc ctgagccggg
     1561 tcgcagcgcc gggcaaacac atcgtcaata tctccagcgg cggcaagatg aaggacacct
     1621 gggtgctcgg aggccaaggg cgatgaacta tggcgagatc ataacgagcg ctttccggat
     1681 cacgctctac aaccgctacc tctggttctt cgggttcttc gtgggcctgg gctcgggcgg
     1741 tggtggcggc ggaggtggcg gtgacttcga cgaacagcct tctgccgaga tcgcctccac
     1801 cgcagcggtc cagcagggag cattcgacaa tgcgctcctc atagtcggcc tggtcctggt
     1861 ggggctactg ctcctcctcg tcttcttcgc gctttacgtg gtctctcagg gagggctaac
     1921 cgagagcgtg gcggcgatag acaggggcga ggaacgtcgc ttctcgtcca cctggagggc
     1981 tggagcccgc aacttctggc ctgtgctgtg gcaggtactg ctttttgtgg ggatcgtgct
     2041 cggtctgctg ctggcggtcg ggataccggt cgcgcttctg gtcgcggggg ctttcgccac
     2101 aggctcgacc gcgctgcggg cggctgccgt cttcgtggcc gtgctcgtcg cgatcgcgct
     2161 gctggtcgtg gtcttcgtgc tactcgccat agtcaggcag ttcgcccggc gggagctggt
     2221 gctgggcggg atagacgtcg tcgggtccgt cgggaggggc taccggctcc tccggcgcaa
     2281 cctcggcagg agcctcctgg tgtggttgat ccagctcggc ctgatgctcg cggccgcgat
     2341 agcgctcctc atcgcgctcg tcatcgtcgg gttattgctg ttcttgccga cgatactcct
     2401 cgcggtcgcg cagtacacca ccgccgcgat agtggcgggc gtggtggcgg gcttgatcct
     2461 gcttccgctc ctcatcgcgg ccctcgccgt cctcggcact ttcaaccatt cttactggac
     2521 gctcgcctac ccgcgactga cgaaccaagg aggtacgacc cgaccatgaa ctacggcgat
     2581 ctcctttcgg aggcattccg cctcgcctgg cgcaaccgtt tcctctggtt cttcgggttc
     2641 ttcgtcgcgg ggggcggcgg ctccttcaac ttcccagcca acttcagcgg ccaggaggtg
     2701 cagaacccgc tcggtccggt cgccggtccg ctgcaatgga tctccgacaa cctcgtcctg
     2761 ttcctcaccg tggttatctc ggttatcgtg gtgctcgcgc tgatcttcct cgtgctctcg
     2821 atgatctcgc agggcgccct cgccgagagc gtcgccgcgc tgcaccgcgg ggagacccgc
     2881 agcttcggct cagccttcag ggtcggtacc gccaacttct ggcgcgtgtt cgggctgaag
     2941 gtgttgttct tcctcatagc tctcggtctt gtactcgtga tcttcctccc cgtggtgctc
     3001 ggcgcgctgg ccgccttctc gctcacggac tcgacggggt tgcgagtcct cgcggtcgtg
     3061 ctcggcgtgc tcttcgtctt cgtggccctc atcgtcgtct tcctgccctt cgccatcgtc
     3121 aaccagttcg gactgcggga gctggtcgtg agccggaggc gcatcacgga gtcgatcgcg
     3181 ggcggcttca ggctcttccg ccggaacatc gggcgcagcc tgctcgtgtg gttgatccag
     3241 ctggccgtca tgcttggtct ggggatagcg ctgctcgtgg cgctcgtcat cttcggggcg
     3301 atactgctcg gacccgcgat agccctcttc gcaaccgacc acgcgaacgc cggcgtggtc
     3361 gctggcgtcc tcggtggcgt cctcttcctg gtgcccgtgt tcgtgatctc gggtgctatc
     3421 ggcgccttca accacgccta ctggacgctg gcgtacctgc gtctagtcga gcctcccgaa
     3481 gacctggagc ccgcgcaggc gagcgcagcg tagatgagaa gatccgacga aaggagttct
     3541 gacatggcga aactcgacgg caaggtcgct gtggtgacgg gcgcttcgag cggcatcggc
     3601 gaggcgacgg cggaagcgct ggcgagggag ggtgcggcgg tcgtcgtcgc tgcgaggcgg
     3661 gaggatcggc tcgcgggtct cgtagagcgc attgagggcg ccggcgggcg cgtgctcgcc
     3721 gccgtctgcg acgtcacgga cgagagccag gcgcacgctc tgatccggaa ggcggtcgag
     3781 gagttcggca gcgtggacat cctggtaaac aacgcgggcg taatgctgct ctcgaccgtg
     3841 gggaagagcc tctccgacca gtggcggcag atgttcgagg tgaacgtcat cgggttgctg
     3901 tacaccaccg acgcggcgat cggacacatg aaggagcagg gctcgggcca cctggtgaac
     3961 atctcctcgg tcgccgggcg taaggtgacc cgtgcctcga gcggggtcta cgccgggagc
     4021 aagcacgccg tggtcgccat ctcggaggga ttgcgtcagg agcttctgga tgacaacatc
     4081 agggtgacgg tagtcgagcc tggggccgtc gccaccgaac taacggacca cataaccgac
     4141 gaggacgcca gggaggccgt tagcggcctc cacgagctcg atatcctgca ctcggaggac
     4201 atcgcagagg ccatcgtcta cgcggtcacc cagccggata gggtgagcgt gaacgaggtc
     4261 ctgatccgcc cgacccagca gcctgtctag cttaggaggg gtggttcgcg aaccgcctcg
     4321 cgcacgaaga ggagcgccgc gcctacgaac gggcgggagc ctggacgaga tctccgccgg
     4381 cggcgcgctc ttcctcggcc tggaggagct tgttcaggga ctggacggcg acctcgagtt
     4441 cggcgcggcc gggctccgtg gttgtgaagt acctctggag gaagttgccg acggagatgg
     4501 aaggccgcgt cttgccgagg acgaaccagg cctcggagat aaggatgaac tggaggatgg
     4561 cgtagcccca ccagccgacg agcgggtcga gcactgcggc gaggacgatg tagccaagga
     4621 tgttcgtgcc gcagcgcgga tgcacgcggc tgctcttccg cgccccttcg agcgttacct
     4681 ccccgtactc ctcgtaggcg gctacggcct tgtgctcggc cccgtggtag cgtccgatcg
     4741 aggtgaactt catcgcagcg aaaaagagga gcaagacggg caggatcggg aactcggcca
     4801 ggaaagcgaa gacgccgccc agggggccag ctccgctccc gagctccatg aggcgcacaa
     4861 agagccagag accggcgagc gaggccccga agaagacaag gacccagaag gagccgacga
     4921 cctgcaagag gagccggcac cagaaaaaca acgagcggat tatgggccag cgggagatgc
     4981 gctcgacggt tggattcttc ggcttccaga tcttcgaagg ctcgacgcgg acgtggatct
     5041 caccttttcg gcggtacgcc acgctcatgt agttcgggcc gaagaggtca aggccgtccc
     5101 agtgggccat gccgccgaag accaggcgct ccttcctatc cggctctgcg gcgtctttcg
     5161 ccaaaacgct ccctctattg tccattaccg aagaacaaaa ggttagcacg ccgtgcggtt
     5221 tttgggctcg tgcggcggcg ggtagaatgg cggaggacga tcaagctccc ccgggagatt
     5281 tcgtgaaagg gtcgagcgac gccgcgcgga agcgcataat ctatgtgagc atcgggctca
     5341 tcttcgcgct cctggttgcc agctacctcg tctaccagat agcggtcgtc gtgctcgtgt
     5401 tgctgctgac gatgctgttc tcggtgatca tcagcgcccc cgtggactac ctggagaaca
     5461 gtgggatgag ccggggcctt ggcacgctcg tggtcttggg cggcctcgtg ctcgggatct
     5521 ggatcgccgg cgtcgcgctc gccccgacca tcgcggatca ggccgtccag ttctgggaga
     5581 ctgtccccga gctactcgaa aacgcgcagg accatgcagg gcagttgcgg gaagcccttg
     5641 gcctcgggag ctcgttcggg ctcggctcgc taaacgtcgt ggattcggcg cgcaactttc
     5701 tctcgggcgg cgcgctgacg acggtggcca acgtcggcgc gggcgtggcg agcgtcatct
     5761 cctacctcgt cgtgatcctc atagccacca tctactccgt cgcgcagccc gggccgctcg
     5821 tcaacgggtt cgtcgccctc tttccagccg ggcggaggca ggaggtgcgg cggatcctcc
     5881 gcgaactgta cgagacgatc cagcggtggt tcgtcggcca gctcgcctcg atgctcctca
     5941 tcggagtcct ttcgaccgta gcgctttacc tgatcggggt gcccttcgcg ctgttgctcg
     6001 ggatcttctc cggcctcatc tccttcatac cgttcgtggg gccactgatc tcggtgatcc
     6061 cgccgttact cctggcgctg atcggcactc ctatagacgc cctctgggtg gtccttgcct
     6121 acgcggcgat ccagactatc gagtcatacc tgatacagcc gctcgtgatg agccgggccg
     6181 tctccctcca ccccgcggta gtgatgttcg cgctgctgat aatggggacc ctcttcggct
     6241 tcgtcggcgt cctgatcgcc gtcccgctcg tggccgcgat cgccgtcctg ctgcgcgagc
     6301 tctggatcga gcgcatggac tctctagg
//
LOCUS       DASTUV010000317         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1289646, whole genome shotgun sequence.
ACCESSION   DASTUV010000317 DASTUV010000000
VERSION     DASTUV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289646"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFT03_06360"
     CDS             <1..531
                     /locus_tag="VFT03_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEU4847802.1"
                     /translation="VGAGTACNDDPRLTVRLVSGDDPLRVVVDSTFRTPLSAAVLAHG
                     AAKRTILAVTDRAPGERCEEARSLGATVLRLPASAAGRVNLGALLAELYSMGVRSVMV
                     EGGAALITSFLYDRLVDRLAVCIAPKILGSGIEAVGDLGIGDLTDSIILTDTSVTPYG
                     VDLVLDSRVEYPDGNR"
     gene            534..1559
                     /locus_tag="VFT03_06365"
     CDS             534..1559
                     /locus_tag="VFT03_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEU4847803.1"
                     /translation="MELSLEARAVWFGGPRTAELRREGVPSPGPGDVRVRSIISAISH
                     GTEMLVYRGEVPPDLPLDLPTLAGDFSFPIKHGYATVGRVAESGTAVERLSPGDLVFV
                     HHPHQDTFTVPAAMPVRLPDGTDPAAGVFFANLETALNVVHDTPLHLGETALVFGQGV
                     VGLLVTQLLKRAGAERVLAVEPRERRRELALQMGADASFEPDEDLLERVLEATGGRGA
                     DVAVEVSSSGAALQAAVDAVAAEGTVVVASWFGTKPVTLSLGGHFHRGRVKVRSSQVG
                     GIPSELGPRWDRARRTEAVLDLLPRLRLAELVSHKVPFEEAPRAYRLVDQAPGETVQV
                     VLTYEGG"
     gene            1561..1938
                     /locus_tag="VFT03_06370"
     CDS             1561..1938
                     /locus_tag="VFT03_06370"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU4847804.1"
                     /translation="MYEVYVAAQFEAAHRLVGDFGPATRMHGHTYRLEVIVRGKRLSG
                     DGTLYDIGRLRPAVEDLASSLHYRDLDEVSGLESVNTTAEAVADYCWERLALTLRDGG
                     LDSLTVRVWESPQVYAARDDSLV"
     gene            1946..3004
                     /locus_tag="VFT03_06375"
     CDS             1946..3004
                     /locus_tag="VFT03_06375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4847805.1"
                     /translation="MRVAFVTVGDTERLTGGYLYNARVLLGLRENGVEIEEIVPCGAS
                     LEEQEGSASRLGTALEPRHFDVIVVDALARIVIAPHLDRWRKERPLVAMVHELPSVAA
                     PKAVLDREREYEEPLLRAESLISVSPHGASILEERGVPAARIRVVPPGFDRLPKGYKT
                     GLPGRNDETVRALCVAQWIPRKGILDLVRAWTLHERSGAVLEFVGETDADPVYAASVR
                     AAIADTPEGSITVSGAVDDATLAVAYATADLFVLPSRFEGYGVVYAEALAHGLPVIAC
                     NVGPVPEVVGEEATLLVPPGDVEALCGALDLLLNDAGLRNRMSAAARRRARELPRWED
                     TAAGFLRVLRELVTERWP"
     gene            3034..>3240
                     /locus_tag="VFT03_06380"
     CDS             3034..>3240
                     /locus_tag="VFT03_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847806.1"
                     /translation="MERKHLREQNRFSWNGAVGAHDSHRGDLAGFLRAGGSTLFPEEL
                     ELLGDLEGKALAHLQCNSGGDSLSL"
BASE COUNT          490 a         1091 c         1117 g          542 t
ORIGIN      
        1 gtcggcgcag gcaccgcgtg caacgacgac ccgcgcctga ccgtccgcct ggtctccgga
       61 gacgaccccc tgcgcgtagt cgtggacagc acttttcgta cccccctctc cgcagcggtg
      121 ctcgcgcacg gggcggcgaa gcgaaccatc ctggccgtca cggaccgcgc cccgggtgaa
      181 cgatgcgagg aggcccggtc tctgggcgca accgttctca ggttgccggc gagcgccgcg
      241 gggcgggtga atctcggcgc gcttctggca gagctttatt ctatgggcgt ccgctcggtc
      301 atggtggagg gaggggcggc cctgatcacc tctttcctct acgaccggct ggtggaccgc
      361 ctcgccgtct gcatcgcgcc gaagatcctc ggcagcggca tcgaggccgt gggcgacctc
      421 ggcatcggcg accttaccga ctccatcatc ctcaccgaca cctccgtcac cccctacggc
      481 gttgacctcg tcctcgacag ccgcgtcgag tacccggacg gtaatcgctg agcgtggagc
      541 tctccctcga agcccgcgcc gtctggttcg gcggtccgag gaccgccgag ctccgccggg
      601 aaggggtgcc gtcgcccgga ccgggcgatg tccgcgtccg ctccattatc tctgcgatca
      661 gccatgggac cgagatgctc gtctaccggg gcgaggtccc acccgacctc ccgctcgacc
      721 tgcccaccct cgccggcgac ttctccttcc cgatcaaaca cggctacgcg accgtcgggc
      781 gagtcgcgga atcgggcacc gccgtcgagc gcctctcccc aggagacctg gtcttcgttc
      841 accacccgca ccaggacact ttcaccgtgc cggcggccat gccggtgcgt ctccccgacg
      901 gcaccgatcc cgccgccggt gtcttcttcg ccaacctgga gaccgcgctg aacgtcgtcc
      961 acgacacccc gctgcacctc ggcgagacgg cgctggtctt cgggcagggc gtggtcggcc
     1021 tcctggtcac gcagcttctc aagcgcgccg gggccgagcg ggtgctggcc gtggagccca
     1081 gagagaggcg aagggagctg gccctccaga tgggagcgga cgcgtccttc gagccggacg
     1141 aggacctgct cgagcgcgtt ctggaggcga ccggcggcag gggcgcggac gtggcggtcg
     1201 aggtgagctc ttcgggcgcg gcgctgcaag ccgccgtgga cgccgtcgcc gccgagggga
     1261 ccgtggtcgt cgcctcctgg ttcggcacga agccggtaac cctctctttg gggggacact
     1321 ttcacagggg gagggtaaag gtgcgctctt cgcaggtagg cgggatccct tcggagctcg
     1381 gtccccgctg ggaccgcgcc cgcaggacgg aggccgtcct cgatctactc cccaggctgc
     1441 gcctcgcgga gctagtctcg cacaaggtcc ctttcgaaga ggcgccacga gcataccggc
     1501 tcgtggacca agctccaggc gagacggtcc aggtggtcct gacctacgaa ggaggatgag
     1561 atgtacgagg tctacgtcgc ggcgcagttc gaggcggcgc accggctcgt cggggacttc
     1621 ggacctgcaa cgaggatgca cggtcacacc tacaggctgg aggtgatagt gcgcggcaag
     1681 agactgtccg gggacggaac cctctacgac ataggccggc tccgccccgc tgtggaagac
     1741 ctcgcctcct cgctccacta ccgcgatctg gacgaggtgt ctggcctcga gagcgtcaac
     1801 acgaccgccg aggcggtggc ggattactgc tgggagaggc tcgcgctgac gctgcgcgat
     1861 ggcggcctcg actcgctcac cgtgcgcgtc tgggagtcgc cgcaggtcta cgctgcccgc
     1921 gacgattctc tcgtctgaac agaccgtgcg cgtcgccttc gtcacggtgg gggatacgga
     1981 gcgtctgacc ggcggttacc tctacaacgc cagggtactc ctcgggttac gagagaacgg
     2041 ggttgagatc gaggagatcg tgccctgcgg cgcttccctc gaggagcaag aagggtcggc
     2101 gagtcgcctc ggaacggctc tggagccacg acacttcgac gttatcgtgg tggacgccct
     2161 ggccaggata gtcattgcac cccacctcga ccgctggcgc aaggagcgtc ccctcgtggc
     2221 gatggtccac gagcttccga gcgtcgccgc tccgaaggcg gtgctggatc gggaacgaga
     2281 atacgaggag ccactgttgc gagccgaaag cttgatctcc gtgagccctc acggagcttc
     2341 gatcctcgaa gagaggggtg tgcccgccgc gcgcatccgc gttgtcccgc ctggcttcga
     2401 ccgccttccg aagggttata agacggggtt gcctggccgt aatgacgaga cggttcgcgc
     2461 cctctgcgtg gcgcagtgga taccgcgcaa ggggatcctg gatctggttc gagcctggac
     2521 gctccacgag cgctcgggcg cggtgctcga gttcgtcggc gagacggacg cagaccccgt
     2581 ctacgcggcc tccgtgcgcg ccgccatcgc ggatacaccc gagggctcga ttaccgtgag
     2641 tggtgcggtg gacgatgcga cgctcgcggt cgcctacgcc accgcggacc tcttcgtgct
     2701 gccttctcga ttcgagggtt acggcgtcgt ctacgccgag gcgctcgccc atggactgcc
     2761 ggtaatcgcg tgtaacgtgg gtcctgtccc cgaggtggtc ggcgaagagg ccaccttgct
     2821 ggtgccgccg ggcgacgtgg aggcgctctg cggggctttg gatctcctcc tgaacgacgc
     2881 cggattgcgg aaccggatgt ccgccgccgc ccgccgccgc gctagagaac tgccccgctg
     2941 ggaggacacc gcagccggct tcctgcgcgt cctgcgagag cttgtaacgg aacggtggcc
     3001 ttgagcgcgc gggctgcggt gtagaatccg gccgtggaac gcaagcatct gagggaacag
     3061 aacaggttct cgtggaacgg ggcggtaggg gcgcacgaca gccaccgcgg ggatctcgcc
     3121 ggcttcctgc gcgcgggtgg gagcactctc ttcccagagg aactggagct tctcggagac
     3181 cttgaaggga aggcgcttgc tcaccttcag tgcaactctg ggggcgactc gttgagcctg
//
LOCUS       DASTUV010000318         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_396403, whole genome shotgun sequence.
ACCESSION   DASTUV010000318 DASTUV010000000
VERSION     DASTUV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396403"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1439)
                     /locus_tag="VFT03_06385"
     CDS             complement(207..1439)
                     /locus_tag="VFT03_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEU4847807.1"
                     /translation="MIDGERLWRRISDLGEIGSQDEGGVTRLSFTDEERAAKDKVVSY
                     MKEAGLSVREDAAGNLFGRREGRNPDSPSVLIGSHVDSVYNGGNFDGPLGVLAGIEVL
                     HAMEEQGIETEHPVEVVAFTDEEGARFSFGMIGSRALAGRLTPEDLTEHEDAGGVSIA
                     EAMRAYGLDPERIGEAARPAGSVKAYVELHIEQGRVLENEDLPAGVVTGIAGPVWLRF
                     SIEGETGHAGATPMSLRRDALAASAEIMGLIEAEASGTGTSVGTVGQLGLEPGGINII
                     PGRVEFSLDLRDIDGEVRDGVETRILEGAKEICRRRGVELEIEPLQRLAPAPCSELVR
                     DAVARACERVGIRAHALSSGAGHDGMQLTELCSMGMIFVRSKNGVSHSPDEWSTKEDC
                     AAGSNVLYWTVLDLAGGG"
     gene            complement(1441..>2169)
                     /locus_tag="VFT03_06390"
     CDS             complement(1441..>2169)
                     /locus_tag="VFT03_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU4847808.1"
                     /translation="DGTLNDINGQHVRAEHLREALADARTGPVTEGSVGGGTGMICHE
                     FKGGIGTASRLTAEEGGWTVGALVQANYGRRHMLRVDGAPVGRALSTERVPSPYEEGD
                     TPAAGTGSIIVILATDAPLLPTQCDRLAQRASIGLGRMGGGCDDASGDIFLAFATGNS
                     GLSGSGEPDPPASIPLTTIPNKAMTPLFYAAAEATEAAILNALLAAGTLTGRDGITAH
                     GLTSDLLLGALDEARSLCARTQGG"
BASE COUNT          324 a          759 c          698 g          388 t
ORIGIN      
        1 tccttgtgct gccgatcgtc ggaagggatg aaaagggaat tagtcaccta ccggcgcagc
       61 acctcctggt aatcagggcc gcgcagctcc tcgaggaaac tggtgatggc agcccagacc
      121 cccgagggca gtaccacgga cgcatccagc atgacgacag cggaggtgag ctctggatag
      181 cgggcggcga ggtcctccct cctgaactag cccccgcctg ccagatctag caccgtccaa
      241 tatagaacgt tgctaccggc agcgcaatct tctttggtgc tccactcgtc ggggctgtgg
      301 ctgacgccgt tcttggagcg cacgaagatc atgcccatcg agcacagctc cgtgagctgc
      361 atcccgtcgt gccccgcgcc gctggagagc gcgtgagctc tgatgccaac cctctcgcaa
      421 gcccgagcca cggcgtcacg gacgagttcc gagcacggcg cgggcgcgag ccgttgcagc
      481 ggctcgatct cgagttccac accgcgcctg cggcagatct cctttgcccc ttcgaggatg
      541 cgggtctcga ccccatccct gacttccccg tctatgtccc tgaggtcgag cgagaactcg
      601 acccggccag ggatgatgtt gatgccaccc ggctcgaggc ccaactggcc cacggtgccc
      661 acgctcgtgc cggtacccga ggcctcggcc tcgatgaggc ccatgatctc cgccgacgcc
      721 gcgagcgcgt cgcgccggag gctcatcggc gtcgcacccg cgtgcccggt ctctccctcg
      781 atcgaaaacc ggagccatac cgggcctgct atgccggtaa ccaccccggc agggaggtcc
      841 tcgttctcca ggaccctgcc ctgttcgatg tgcagctcga cgtaggcttt cacagaccct
      901 gcgggacggg ccgcctcgcc gatgcgctcg gggtcgagcc cgtacgcgcg catcgcctcc
      961 gctatggaga cgcctcctgc gtcctcgtgc tccgtcaggt cttccggggt gagcctgcct
     1021 gccagagcgc ggctcccgat catgccgaat gagaagcgcg caccctcctc gtccgtgaag
     1081 gcgacgacct cgacgggatg ctcggtctct attccctgct cttccatcgc gtgcaggacc
     1141 tcgatgcccg ccagcacgcc cagaggaccg tcgaagttcc cgccgttgta cacggagtct
     1201 acgtgggagc cgatgaggac ggaaggtgaa tccgggttcc ttccctcccg cctgccgaac
     1261 aggttgcccg cggcgtcctc gcggaccgag aggccggctt ccttcatata cgagacgact
     1321 ttgtccttcg cggcccgctc ctcgtccgtg aaggacaacc gggtcacgcc gccctcatcc
     1381 tggctgccta tctcgccgag gtcggatatc ctgcgccaca gccgctcacc gtcaatcaca
     1441 tcatcctcct tgtgtcctgg cgcacagaga gcgggcctcg tcgagcgcgc cgagcaggag
     1501 gtcggaggtg agcccgtgcg ccgtgatgcc atctcgccct gtcagggtgc cggcggcgag
     1561 gagggcgttc aggatcgccg cctcggtcgc ttcggcggca gcgtaaaaga gcggggtcat
     1621 ggccttgttt ggaatggtgg taaggggaat gcttgccggt gggtccggtt cgccggaccc
     1681 ggagagaccg ctgttgcccg tggcgaaggc gaggaagatg tcgccgctcg cgtcgtcgca
     1741 gccgccaccc atccggccga ggccgatgct cgcgcgttgg gcgagccggt cgcattgtgt
     1801 cggtagcagc ggcgcgtccg tggcgaggat cacgatgatc gagcctgttc cggctgccgg
     1861 cgtatcccct tcttcgtatg gcgaggggac gcgctcggta ctcagggcgc gtccaacggg
     1921 cgcgccgtcc acgcgcagca tgtgccggcg gccgtagttc gcctggacga gggctccgac
     1981 ggtccagccg ccctcctccg cggtcagccg ggacgccgtg ccgatgccac ccttgaactc
     2041 gtggcagatc atgccggtcc ctccgcctac cgagccttca gtcaccggtc cggtacgcgc
     2101 gtccgccagc gcttcgcgca ggtgttcggc tctgacgtgc tggccgttta tgtcgttgag
     2161 ggtgccgtc
//
LOCUS       DASTUV010000319         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_185752, whole genome shotgun sequence.
ACCESSION   DASTUV010000319 DASTUV010000000
VERSION     DASTUV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185752"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VFT03_06395"
     CDS             complement(<1..1301)
                     /locus_tag="VFT03_06395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4847809.1"
                     /translation="MKLIMTHGYMLSDTGSNVYVQSLCRALAREGHEVHLLCQEPEPL
                     SYDFVDEWAVVDEGSIDKRGEQQTPCPGRCTVFRPEIGDLLPVYVYDDYPGWRVKTFL
                     DLTDDELENYVEKNIGAVRTVLAASGAEAVVTNHAVPGPLIARRALEGADVPYSSIIH
                     GSALQYVSRKSEKYMRLTREGLDKAKNILALSSHSAGTIAEDFPDFAGKTSALPGGVD
                     TDLFRPDALDLSILKKLSGGPGRGPEQRAALKEAIGGSASADELLGTLRGISASYDAR
                     SHDEDAGERLERFISGDAPLVVYVGKLIHSKGVHSLLSAFARVRIETGARLLVIGFGT
                     FREGLEALALALSTGDEKTVERLAELGKLLEGGQAGPLEHFEVSQELLQDAAGMEDDV
                     EFIGPLRHEELTRLLPAAELGVVPSIFPETFGLVAAELAASG"
     gene            1420..>2029
                     /locus_tag="VFT03_06400"
     CDS             1420..>2029
                     /locus_tag="VFT03_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEU4847810.1"
                     /translation="MRARRSPESRLEPPRALEGWDAVPVAAPLADAAEAARIRRSWTS
                     IRVPSHWQLEDAFAAYEGLVLYRCRFAWRSDEQEMMFALRFGGVYYSARVWLNGTYLG
                     GHEGYLSPFELDVTDLLVAGENELLVEVHSPEEREENERATIGGVWARWDGLGPHINP
                     GGIFRAVELLSSGEVRIRSLGAAVDGSGYGRAHVEMYARRGVL"
BASE COUNT          340 a          627 c          703 g          359 t
ORIGIN      
        1 ccgctcgctg cgagctccgc agccacgagc ccgaaggtct cggggaagat ggagggcacg
       61 accccgagtt cggccgccgg caggagcctg gtgagctcct cgtgacgcag cgggccgatg
      121 aactctacat catcctccat cccggccgcg tcctgcagca gctcctggga cacctcgaag
      181 tgctcgagcg gtcccgcctg gcctccttcg agcagcttgc ccagctccgc cagcctctcg
      241 acagtcttct cgtcacccgt gctcagggca agggcaaggg cttcgaggcc ctcgcggaag
      301 gtgccgaagc cgatcacgag tagacgcgcg cccgtctcta tgcgcacccg cgcaaaggcc
      361 gagaggagac tgtggacgcc cttggagtgg atgagcttgc caacgtagac cacgagcggg
      421 gcgtcgcctg agataaagcg ctcgagccgt tcgccggcat cttcgtcgtg cgagcgggcg
      481 tcgtaggagg ctgagatccc gcggagcgtt cccagcagct cgtcggcgct cgccgagccc
      541 ccgatggcct ccttcagggc tgctcgttgt tcgggaccgc gcccgggacc gcccgagagt
      601 ttcttcagga tggagaggtc gagcgcgtcc ggtctgaaga gatccgtgtc cacgccgccg
      661 ggcagcgccg aggtcttgcc ggcgaagtcg gggaagtctt cggcgatggt gcccgcgctg
      721 tgggaggaga gggccaggat atttttcgct ttatccagtc cttcgcgggt gagcctcatg
      781 tacttctcgc tcttgcggga gacgtactgc agggcgctgc cgtggatgat gctcgagtag
      841 ggaacatccg cgccttcgag ggcgcggcgc gcgatcaggg gccccggcac ggcgtggttg
      901 gtgacgaccg cctccgcgcc ggaagccgcg agcaccgtcc gtaccgcgcc gatgttcttc
      961 tcgacgtagt tctcgagctc gtcatcggtc aggtcgagga aagtcttgac cctccagccg
     1021 gggtagtcgt cgtagacgta gacgggcaac aggtcaccga tctccggccg gaagacggtg
     1081 cagcgccccg gacagggcgt ctgttgctcg cctcgcttat cgatgctgcc ctcgtccacc
     1141 acagcccact cgtctacgaa gtcgtaggag agcggctcgg gttcctggca caggaggtgg
     1201 acctcgtggc cctcgcgtgc cagggcgcgg cagaggctct gcacatagac gttgctcccg
     1261 gtatccgaga gcatgtagcc gtgggtcatg atcagcttca cgataccccc tcagggcgac
     1321 cgaaccagag ccaccattct cgcacagagg gaccgaacta tggccatgag tttactcgag
     1381 gggccggcga gctagattcc gtgctacggt agcagcatcg tgagagcgag gagatcaccg
     1441 gagagcaggc ttgaacctcc gcgagcactc gagggctggg acgccgtgcc cgtcgccgcg
     1501 ccgctcgccg acgcggccga agcggcgaga ataagacgct cctggacgtc catacgcgtt
     1561 ccgagtcact ggcagctcga agacgccttc gcggcgtacg aggggctcgt cctgtacagg
     1621 tgccggttcg cgtggcggtc cgatgagcag gagatgatgt tcgctttgcg gttcgggggc
     1681 gtatattact ctgcgcgggt ctggttgaac gggacttatc tgggagggca cgaaggttat
     1741 ctctctccct tcgagctcga cgtcaccgat ctcctcgtag ccggcgagaa cgagctcctg
     1801 gttgaggtgc acagtcccga ggagcgcgag gagaacgaga gggcgaccat cgggggggtg
     1861 tgggctcgct gggatggatt gggaccccac ataaaccctg gcggcatctt cagggcggta
     1921 gagcttctct cgagcgggga ggtgcgcatc agatctctgg gagccgccgt ggatggttcg
     1981 ggatacggaa gggcgcacgt cgagatgtat gcccgccggg gagtactga
//
LOCUS       DASTUV010000320         8330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1718602, whole genome shotgun sequence.
ACCESSION   DASTUV010000320 DASTUV010000000
VERSION     DASTUV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718602"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VFT03_06405"
     CDS             <1..1114
                     /locus_tag="VFT03_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU4847811.1"
                     /translation="ELSSTTSTSINLPFITADQNGPKHMDLTLTRAQFNNLTADLVEA
                     TAGPVRQAMEDAGLQSGQVDQVILVGGSTRIPAVQEQVKEITGKEPHKGINPDEVVAI
                     GAAIQAGVLAGEVKDVLLLDVTPLSLGIETKGGVFTKLIERNTTIPTRKAEVFTTADD
                     NQNSVEIKVYQGEREIAAYNKLIGNFQLVGIPPAPRGVPQIEVSFDIDANGIVNVGAK
                     DLGTGKEQRITITASSGLSESEIEQMVRDAESHAEEDQRRREEADVRNNADNLVYSIE
                     RSLKDVDGKVDPNTKLEIEQAIKEAKEALAGGDIEEIKAKQEALVAASHKLSEVLYSQ
                     AQEQQQASSSGATDDDDVVEDAEIVDDDEEEDDQRK"
     gene            1182..1796
                     /locus_tag="VFT03_06410"
     CDS             1182..1796
                     /locus_tag="VFT03_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU4847812.1"
                     /translation="MSSEENRREPEAREEPEALEGIDEAQGSPGEPVEEDPGTPEEPE
                     GPPVEEATEEELAALSAELEVTRRERDEYLDALRRLKAEFDNSRKRLERERSRILQAA
                     SERLVVELLPVLDNLDRALEAEGDIREGVYATRDHLVEVLQSEGLVPVASDGQPFDPN
                     VHEAVMGQPSEDHEEGTILQTFQRGYVLNGKPIRVAKVVVARQG"
     gene            1800..2906
                     /gene="dnaJ"
                     /locus_tag="VFT03_06415"
     CDS             1800..2906
                     /gene="dnaJ"
                     /locus_tag="VFT03_06415"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU4847813.1"
                     /translation="METGDLYKVLGVTREASQEEIRRSYRKLAREHHPDANRDDPKAE
                     ERFKEIQHAYEILSNPERRRQYDEGPRAFFGGPGQGTAGSTRVDDLSDLFGGFGGLGD
                     IFGRSTRATTASRKGQNITATVNLSFKDALNGVTTRVNVPVEEACGDCGGMGAAPGTS
                     PRTCPECGGRGVRSRDQGFFAFSEPCVRCGGEGTIVEKPCRTCSGSGRVRTARQVTVR
                     IPAGAKDGMKIRVPGRGSAGQRGGPAGDLYVVTRVGEHPVFKRRGDDFVVEVPVSFVE
                     AALGAQIQVPRPGGGTVKLRLPAGTQDGKQFRVRGAGAPKARARSGERGDLIVRARLV
                     VPRKLKRREREILEALAEERDEDVREDLFKKVGS"
     gene            2903..3298
                     /locus_tag="VFT03_06420"
     CDS             2903..3298
                     /locus_tag="VFT03_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4847814.1"
                     /translation="MKLRKRPVFMIGIASELIGVHPQTLRMYEQKGLLRPRKSIKKTR
                     LYSEEDVELGRYIQKLTQEMGMNLAGVKKVLDLERRIATLEAEKSQLREELERREQEH
                     SRVVAEVHRSYRRELVLMPRGDLARGGGS"
     gene            3298..3783
                     /locus_tag="VFT03_06425"
     CDS             3298..3783
                     /locus_tag="VFT03_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847815.1"
                     /translation="MSETMHCYRHPARETRVSCATCGRPICTECMVPTDVGIKCPDDA
                     RLPRGARAGVMKGDQIARSLLAGAAVVLLGAVIVYFVLPQIGFGRVILSGLAGYFAGM
                     FVHRAGGRNGGPIAMAISGVSVALAFVPLLAQAVLNGNPPLYLLLPALIAVGFAVYNS
                     R"
     gene            3800..6232
                     /locus_tag="VFT03_06430"
     CDS             3800..6232
                     /locus_tag="VFT03_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4847816.1"
                     /translation="MDLNRFSQNAREVLDVATAVVRRGRSNQLGTEHVLLGLLAQQGG
                     VVHRVFEELGLDLGLAQAKTNDAIRRNDLSRSRIAAERVQTTYNARNALRLAGEEAEK
                     SASDHIGTEHLLLGLVLVEEGTAAGVLREVGLTETDLRRALGRVRGEASLGEDPDSLL
                     GRHGRNITRLAREGKLDPVVGREAEIKRMLQILARRTKNNPALIGEPGVGKTAIVEGL
                     AQKMAKGYVPEALKDKVLVSLDLGSLVAGTKFRGEFEERLKGVIDEVKSSYGRIILFV
                     DELHNLAGAGAAEGSIDAGEILKPALARGEVQVIGATTIDEYRRHIEPDAALERRFQP
                     ILVEEPTEEETVEILRGIRDKYESHHGVEISEEALRAAASYSHRYIADRFLPDKAVDL
                     LDEAASQLRIETTMLPDDLRELELKLQEITHEGAAAVEEHDYELAAELRSEADVLQAG
                     YREARERWLEESGVKDATVGREEIARVVSARTGIPVTRMLKDEAERLLSMEKSLHERV
                     VGQDAAVVAVSEAVRKARAGLANPSRPIGSFIFLGPTGVGKTELARALAEYLFDDEDA
                     MIRLDMSEYMERHTVSRLVGAPPGYVGYDEGGQLTEAVRRRPYSVVLLDEIEKAHPDV
                     FNMLLQLLEDGRLTDNKGRTVSFDNTVVIMTSNAGAHLIPGSEEMRGREEEIREQLMR
                     ELQTFFRPEFLNRVDEIIVFHTLGGEELKKIAALLLENLRRTAKDQDIDLRFSDAVIE
                     RIAQEGQGSRYGARPLRRAVGKLIESPLSKEIIAGRVNPGEIVHIDLDPTGHTTFEPA
                     FPATQPTGVETA"
     gene            6477..7016
                     /locus_tag="VFT03_06435"
     CDS             6477..7016
                     /locus_tag="VFT03_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847817.1"
                     /translation="MREDMQYISGVLDELDAIVRDASGVPIRKGRAVVDRSDLLVMLD
                     ELRGSLPPELAEAEEIRRECGAIVAAAEEEAERIIENAHHRADAMVPGTELCRRADRR
                     AREILEGAESYAEEVADGSEVYRDRVMGQLEGWFQDSLVSVGESRQELNGVPVPRPTR
                     TEETEDNEDNGRGWQVTSA"
     gene            complement(7234..8004)
                     /locus_tag="VFT03_06440"
     CDS             complement(7234..8004)
                     /locus_tag="VFT03_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847818.1"
                     /translation="MEPERRDLDRSTPRPLSSAERGRVVRAAALRPLNVVVLVIGAGI
                     FATTLAWWMVPLTLVTYALLVFFASRDPLFARRVLYGREEAGLSRPTADRDVSPERRA
                     RWLPRGETREKVEAALVVYRKVVDAIEESDEVTRAVLDDAVPKLHAAANRLVDVAQRR
                     EKAAEVLGDLDLHTAASSAREDDSEALRERLRAADAEISETFDKLLALRSRVVRVSID
                     SGSPEGAAALNASLDELNARLEALSETMAPPEPPSQRR"
     gene            complement(7994..>8330)
                     /locus_tag="VFT03_06445"
     CDS             complement(7994..>8330)
                     /locus_tag="VFT03_06445"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HEU4847819.1"
                     /translation="PGGEEVGDGEAVTAHIISVDRYGNARLSISQEEWGLEYGATVGA
                     DVGEGEMDVRYLETFGSARTGELVLVPDSHWRLSLAINKGNAARALGLKLGGKVRIGL
                     REGHAGDGA"
BASE COUNT         1602 a         2613 c         2843 g         1272 t
ORIGIN      
        1 ggagctctcg tcgaccacaa gtacctcgat aaacctgcct ttcattacgg cagaccagaa
       61 cggtccgaag catatggacc tgacgctcac gagggcgcag ttcaacaact tgaccgctga
      121 cctcgtcgaa gccacggccg gtcctgttcg ccaggcgatg gaggacgcgg ggttgcagag
      181 cgggcaggta gatcaggtca tcctggttgg cggctcaacg cgcattccgg cggtccagga
      241 gcaggtcaag gagataacgg gcaaggagcc ccacaagggc atcaaccccg atgaggtcgt
      301 cgcgataggc gccgccattc aggctggcgt gctcgctggc gaggtcaagg acgtgctgct
      361 cctcgacgtt acgcccttga gcctcgggat cgagaccaag ggcggagtgt tcaccaagct
      421 catcgagcgc aacacgacca tacccacgcg caaggccgag gtcttcacga ccgcggacga
      481 caaccagaac tcggtcgaga tcaaggtcta ccagggcgag cgcgagatag cagcctacaa
      541 caagctcatc ggcaacttcc agctcgtagg catcccaccg gctccgcgcg gcgtgcccca
      601 gatcgaggtc tctttcgaca tagacgccaa cggcatcgtc aacgtcgggg ccaaggacct
      661 cggtacaggc aaggagcaga ggatcactat caccgcctcc agcggcctct cggagagcga
      721 gatcgagcag atggtccgcg acgccgagtc gcacgccgag gaggaccagc gccgccgcga
      781 ggaggccgac gtccgcaaca acgccgacaa cctggtctac tcgatcgagc ggtccctgaa
      841 ggacgtggac ggaaaggtag atccgaacac caagctcgag atcgagcagg ccatcaagga
      901 ggctaaggag gcgctggccg gcggcgatat agaggagatc aaagccaagc aggaggcgct
      961 cgtggccgcc tcccacaagc tctcggaggt tctctacagc caggcccagg agcagcagca
     1021 ggcgagctcc tccggtgcca ccgacgacga cgacgtcgtc gaggacgccg agatcgtcga
     1081 cgacgacgag gaggaggacg accagcgcaa gtagcgcatc ccggaacggc cggggcacca
     1141 ccccggccgc tcctagcttc aacgggagga agtcgagagc attgagcagc gaagagaaca
     1201 ggcgcgagcc ggaagcccgc gaagaaccgg aggccctcga ggggatcgat gaggcccagg
     1261 ggtctcccgg agagcccgtc gaagaagacc cgggcacgcc ggaagaaccc gaaggcccac
     1321 cggtagagga ggccaccgag gaggagctgg ccgcgctgag cgcggagctc gaggtgaccc
     1381 gccgcgagcg tgacgagtac ctcgacgccc tccgccgcct gaaggccgag ttcgataact
     1441 ctcgcaagcg cctggagcgc gagagatccc gcatcctcca ggcagcctcc gagaggctag
     1501 tcgtcgagct cctacccgtc ctcgataacc tggaccgcgc gctcgaggcc gagggcgata
     1561 tccgcgaggg cgtctacgcc acccgcgacc atctcgtcga agtgctccaa agcgagggcc
     1621 tcgttcccgt agcctccgat ggccagccct tcgaccccaa cgtccacgaa gccgttatgg
     1681 gccagccttc cgaagaccac gaggagggca ccatcctcca gaccttccag cgggggtacg
     1741 tactcaacgg caagcctatc cgcgtcgcga aggttgtagt tgcgagacag ggttagagaa
     1801 tggagacggg ggatctctac aaggttctag gggtaaccag ggaggcgtcg caggaggaga
     1861 tcaggcgctc ctaccgcaag ctggcgcgcg agcaccaccc tgacgctaac cgcgacgacc
     1921 ccaaagccga agagcgcttc aaggagatac agcacgccta cgagatcctc tctaaccccg
     1981 aaagaaggcg gcaatacgac gaaggaccac gcgccttctt cggcggcccg ggacagggaa
     2041 ccgccggcag cacccgcgtg gacgacctct ccgacctttt cggcggcttc ggcggcctgg
     2101 gagacatctt cggacgctcc acccgcgcta ctacagcttc gcgcaaaggc cagaacataa
     2161 ctgcaaccgt caacctcagc ttcaaagatg ctcttaacgg cgtcacgacg cgggtgaacg
     2221 tgccggtcga ggaggcgtgt ggggactgtg gggggatggg tgcggcgcct ggcacgtcgc
     2281 cgcggacttg tccggagtgt ggggggcgtg gggtacggag tcgtgaccag gggttctttg
     2341 ctttctcgga gccgtgcgtg aggtgtggtg gggaaggtac gatagtcgag aagccgtgtc
     2401 ggacgtgttc ggggagtggc agggtacgca cggcgcggca ggtgacggtc aggatccctg
     2461 ctggggcgaa ggatgggatg aagatccggg ttccggggag gggcagcgct ggccagaggg
     2521 gtggtcctgc gggggacctg tacgtggtaa cgcgtgtagg ggagcacccg gtcttcaagc
     2581 ggcgcgggga cgacttcgtt gtcgaggtgc cggtcagctt cgtggaggcg gctctcggcg
     2641 ctcagataca ggtaccgcgt ccggggggtg gaacggtgaa gttgaggctg ccggcgggga
     2701 cgcaggacgg caagcagttc agggtgcggg gtgcgggggc tccgaaggcc agggcgcgaa
     2761 gcggggagcg tggggatctc atcgtgcgcg cgcggctcgt agtgcccagg aagctaaaga
     2821 gacgggaacg ggagatactc gaggccctcg cggaggagag ggacgaggac gtcagagagg
     2881 acctgttcaa gaaggtggga tcgtgaagtt gcgcaagagg ccagtgttca tgatcgggat
     2941 cgcctctgag ctcatcgggg tgcacccgca gactttgagg atgtacgagc agaagggtct
     3001 gctccgcccg cgcaagagca taaagaaaac gcggttgtac tcggaggagg atgtcgaact
     3061 cggacgctac atccagaagc tgacgcagga gatggggatg aacctcgccg gggtcaagaa
     3121 ggtgctggac ctcgagcggc ggatagccac gctcgaggcc gagaaaagtc agctgcgcga
     3181 ggagctcgag aggcgtgagc aggagcacag cagggtagtt gccgaggttc accgcagcta
     3241 ccggcgggag ctggtcctga tgccgcgcgg cgatctcgcg cgcggaggag gatcctgatg
     3301 tccgagacca tgcactgcta ccggcacccg gccagggaga ccagggtctc gtgcgcgacg
     3361 tgcgggcgtc ccatatgcac ggagtgcatg gttcccacgg acgtcgggat caagtgcccc
     3421 gacgacgcaa ggctcccgcg cggcgcgcgg gccggggtca tgaaggggga tcagatcgcc
     3481 aggagcctgc tcgccggagc cgcggtcgtg ctgcttggcg ctgtaatcgt gtatttcgtg
     3541 ctcccgcaga tcggcttcgg gcgggtgatt ctctctgggc tggccggcta cttcgccggg
     3601 atgttcgtcc atcgggccgg tggacgtaac ggcggtccga tcgcgatggc gatctccggt
     3661 gtctcggtcg ccctggcctt cgtcccgctc ctggcgcagg ccgtcctgaa cggcaacccg
     3721 ccgctctact tgctgcttcc ggcgctcata gccgtcggct tcgccgtcta caacagcaga
     3781 tgaaggtggg aggcgccgcg tggacctgaa cagattttcc cagaacgccc gcgaagtgct
     3841 ggacgtggcg accgcggtcg tgcgccgggg gaggtccaac cagcttggca cggagcacgt
     3901 gctacttggg ctcctggcac agcagggggg cgtggttcac cgcgtcttcg aggagttggg
     3961 cctggatctc ggcctcgccc aggccaagac gaacgacgcc ataaggcgca acgacctctc
     4021 gcgctcgcgc atcgcagcgg agagggtgca gacgacctac aacgcgagga acgctctcag
     4081 gctcgccggc gaggaggccg aaaagagcgc cagcgaccat atcggtacgg agcatctgct
     4141 gctgggtctg gtcctggtgg aggagggcac ggccgccggc gtgctgcgcg aggtgggcct
     4201 caccgagacg gacctccgac gcgcgctggg ccgggtgagg ggggaggctt ctctaggcga
     4261 agaccccgac agcctgctcg gcaggcacgg ccgcaacatc acccggctcg cccgcgaggg
     4321 caagctcgac cccgtcgtcg gtcgagaggc ggagataaag cggatgctcc agatcctggc
     4381 tcgccgcaca aagaataacc ctgctctcat cggcgagccc ggggtaggca agaccgccat
     4441 cgtcgagggg ctcgcgcaga agatggccaa gggctacgtc cccgaggcgc tcaaggacaa
     4501 ggtcctggtc tcgctcgacc tcggctcgct ggtggcgggc accaagttcc ggggcgagtt
     4561 cgaggagcgc ctcaaaggcg tcatagacga ggtaaaaagc tcctacggcc gcatcatcct
     4621 gttcgtggac gagctgcaca acctggccgg cgccggcgcc gccgagggct ccatagacgc
     4681 gggcgagatc ctgaagccag ccctcgcccg cggcgaggtg caggtgatcg gagcgaccac
     4741 catagacgag taccgccgtc acatagagcc cgacgccgcc ctcgaacgcc gcttccagcc
     4801 tatcctcgtg gaagagccca ccgaggagga gaccgtcgag atattgcgcg gtatccggga
     4861 caagtacgag tctcaccacg gcgtggagat ctccgaggag gcgctgcgcg ccgccgcgag
     4921 ctactcacac cgctacatag ccgaccggtt cctccccgac aaggctgttg acctcctcga
     4981 cgaggccgcg agccagctcc gcatcgagac gacgatgctc cccgatgacc tgagagaact
     5041 cgagctgaag ctccaggaaa taacccacga gggggcggcg gcggtcgagg agcacgacta
     5101 cgagctcgca gcggagctgc gctccgaggc cgacgtgctg caggccgggt acagagaggc
     5161 gcgcgagcgc tggctggaag agagcggcgt caaggacgcg acggtgggga gggaagagat
     5221 cgcgcgcgtc gtcagcgccc ggaccggcat ccccgtcacc aggatgctca aagatgaggc
     5281 cgagcgtctc ctctcgatgg agaagtctct ccacgaacgc gtggtcggcc aggacgcggc
     5341 ggtcgtcgcc gtctcggagg ccgtcaggaa ggcgcgcgcc ggcctggcga acccaagccg
     5401 gcccataggc agctttatct tcctcggccc gacgggcgta ggcaagaccg agctcgccag
     5461 ggctctcgcc gagtacctct tcgatgacga ggacgccatg atccggctcg acatgtccga
     5521 gtacatggag cgccacacgg tgtctcgctt ggtaggcgcc cctccaggct acgtcggcta
     5581 cgacgagggc ggccagctca cagaggccgt gcgacgtcgt ccctattcgg tggtgctcct
     5641 cgacgagata gagaaggccc acccggacgt cttcaacatg ctcctccaac tcctggagga
     5701 tgggcgcctg acggacaaca aaggccggac ggtctccttc gacaacacag tggtgatcat
     5761 gacgtccaac gccggagctc acctcatccc cggctccgag gagatgcgcg gtcgggagga
     5821 ggagatccgc gagcaactca tgcgcgagct gcagaccttc ttccggcccg agttcctgaa
     5881 ccgggtcgac gagatcatag tcttccacac cctcggcggc gaggagctga agaagatagc
     5941 agccctcctg ctagagaacc ttcgccgcac cgcgaaagac caggacatag acctccgctt
     6001 ctcggacgcc gtgatcgaac gcattgccca ggaaggccaa ggttcccgct acggcgccag
     6061 gcccctccgc cgcgccgtcg ggaagctgat cgagtctccc ctcagcaagg agatcatcgc
     6121 cggccgcgtc aacccgggag agatcgtcca catagacctc gaccccaccg gacataccac
     6181 tttcgagcct gccttccccg cgacacagcc gacaggggtc gaaactgctt gataccccct
     6241 tacctgctac aaaggttaag cctgctcgct cgaaaatccc ctgtggataa ctctcaaaac
     6301 cctttatata aagcgcaaat agccggcgcc gaaatcacga gtcgcagcct ctgtacactc
     6361 cagcaggctt agacttgacc ttaagattat agtgaacctt aacttgaggt aacgcatcga
     6421 aaaatacttt cagaaaacct agcgtagcct gatgaggcgt gctagggttc gtagtcatga
     6481 gagaggacat gcagtacata agcggggttt tggacgagct ggatgcgatc gttcgcgacg
     6541 cttctggcgt accgatacgt aaaggtcggg cggtggttga tcggtccgat ctgcttgtga
     6601 tgctggacga gctaagggga tcgttgcccc ctgagttggc ggaggccgag gagatccgcc
     6661 gggagtgtgg ggccattgtg gcggcggccg aggaggaggc cgagcgcatc atcgagaacg
     6721 cgcaccatcg cgcggacgcc atggttcctg gtaccgagtt gtgccggcgt gcggatcgcc
     6781 gggcgcgcga gatcctggaa ggggcggaga gctacgccga ggaggtggcc gacggttcgg
     6841 aggtgtaccg ggaccgcgtc atgggccagc tcgagggttg gttccaggac tcactcgtct
     6901 ccgtcggaga gtcgcggcag gagctgaacg gcgtccccgt gccacgtcct actcgcaccg
     6961 aggagaccga ggataacgag gacaacggcc gcggctggca ggtgacctcg gcctagagcc
     7021 ggtaccggcc agatccagcg ccgacccgtc cgggtggcgc cggaatgtct ctatgcgagc
     7081 tctctcgctt ctgacctcgc gccctgcgca actacgaccg gcactcattt ctaccttccg
     7141 attcgccctg ctaacgtagc ggcgctggac agctggcgtg ctctctacag gggtgatcta
     7201 tgaggagagg gcttgcgggc cctgtggcgg gcgtcagcgg cgctgtgagg ggggctctgg
     7261 aggggccatg gtttcgctca gggcttcgag ccgggcgttc agctcgtcga gcgaggcgtt
     7321 gagtgccgcc gccccttcag ggctcccgct gtctatggag acccgtacga cccgggatct
     7381 aagggccagc agcttgtcga aggtctcgga gatctcggcg tccgccgcgc ggagcctctc
     7441 tcgcagagcc tcggagtcat cctcccgggc gctcgaggca gcggtgtgca gatccaggtc
     7501 tcccaggacc tcggctgctt tctcccttct ctgggcgacg tccacgagcc tgttggcggc
     7561 ggcgtggagc ttcgggacgg cgtcgtcgag gacggcgcgc gttacctcgt cggactcctc
     7621 gatggcgtcg acgaccttgc ggtagaccac gagcgccgcc tcaaccttct ccctggtctc
     7681 tccccgcggc aaccagcggg cgcggcgctc gggggagacg tcccggtccg cggtgggcct
     7741 cgacaagccc gcctcttcgc gcccgtagag gacgcggcga gcaaataacg ggtccctcga
     7801 ggcgaagaag acgagcagcg cgtacgtcac gagggtgaga gggaccatcc accacgcgag
     7861 ggtggtcgcg aagatgccgg ccccgatcac cagcacgaca acgtttaggg gccgcagggc
     7921 tgctgcgcgc acgacccggc cccgctcggc agatgagagg ggccggggcg tgctgcgatc
     7981 caggtcccgg cgctcaggct ccatcgcctg cgtgaccttc ccgcagcccg atacgaacct
     8041 tcccgcccaa cttgagcccg agcgcacggg cggcgttgcc cttgtttatg gctaggctca
     8101 accgccagtg cgagtccggg acgagcacga gttcgcctgt cctggccgac ccgaaggtct
     8161 ccaggtagcg tacatccatc tcgccttcgc cgacgtccgc ccccacggtg gcgccgtact
     8221 cgagccccca ttcctcctgg ctgatggaca gccgggcgtt gccgtaacgg tccacggaga
     8281 tgatgtgagc cgttacggct tcgccgtccc caacctcctc gcctcctggc
//
LOCUS       DASTUV010000321         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1761758, whole genome shotgun sequence.
ACCESSION   DASTUV010000321 DASTUV010000000
VERSION     DASTUV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761758"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /gene="tig"
                     /locus_tag="VFT03_06450"
     CDS             complement(<1..1209)
                     /gene="tig"
                     /locus_tag="VFT03_06450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU4847820.1"
                     /translation="MAANVTKLEDNKVRLDVEVPPEAVRAGVEAKVRELGRQVRVPGF
                     RPGKAPRRVIENHLGRDYIYYEALQERLPTWYSEAVVETDLRPIDRPEIHFDEPLDEE
                     EGFKFSATVAVRPEASLGEYRGVEVPRREAEVSEEQVDEQLEQMRGQFATLAAVEDRP
                     VGDGDFVIIDFRGERMTTGEPLEGGEAEDYMLEVGRGELLPDFEENLIGMKPGERKQF
                     GVTFPADYAEESLRNQPVLFHVHVKEIKTRDLPPLDDEFAKEASEFETLEELRSAVRE
                     QLEEAAERRAEGEFRTRVLDAVAENVDVEVPDSMVEEKVDEMLGSFERSLQAQGMEPG
                     QYYQLAGTSAEDVKERVRPDAADSVRKELALDAVVAAEGIEADEHEVMHQIEHLAEDS
                     DRDPQEIVEVM"
     gene            complement(1260..1340)
                     /locus_tag="VFT03_06455"
     tRNA            complement(1260..1340)
                     /locus_tag="VFT03_06455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1305..1307),aa:Leu,seq:tag)
     gene            complement(1497..>2045)
                     /locus_tag="VFT03_06460"
     CDS             complement(1497..>2045)
                     /locus_tag="VFT03_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU4847821.1"
                     /translation="INAAYSYGGIEETMGALQGYAQVPIDHYAVVDFEGFRDVINAMG
                     GVEIDVEDEIPPKYGIKDGIQTLNGHQALFYARYRGTAGGDMDRIEHQQRLIAALRSK
                     AFGWDTVTKLPHIARVMNRNVETNLDFDEALSLGRLLISREHAQMTASQLKGTPKTLP
                     NGEEVLVPDQEANEAILAQFRY"
BASE COUNT          316 a          714 c          598 g          417 t
ORIGIN      
        1 catgacctcg acgatctcct gagggtccct gtccgagtcc tcggcgaggt gctcgatctg
       61 gtgcataacc tcgtgctcgt cggcctctat gccctcggcg gcgacgaccg catcgagcgc
      121 gagctccttc ctgacgctgt ccgcagcgtc tggccgcacc cgctccttga cgtcttcggc
      181 ggaggtgccg gcgagctggt agtactgacc tggctccatg ccctgggcct gcaggctgcg
      241 ctcgaagctc ccgagcatct cgtctacttt ctcctcgacc atggagtcgg ggacctcgac
      301 gtccacattc tcggctacgg catcgagcac gcgggtacgg aactcgcctt cggcgcgccg
      361 ctcggcggct tcctcgagct gctcacgcac cgcggagcgc agctcctcca gcgtctcgaa
      421 ctcgctagcc tccttggcaa actcgtcgtc gagaggcggc aagtcacggg tctttatctc
      481 cttgacgtgc acgtggaaca ggacaggctg gttcctgagc gactcctctg cgtagtccgc
      541 gggaaatgtg accccgaact gcttgcgctc tcctggcttc atcccgatga ggttctcttc
      601 gaagtcaggg agaagctcgc cgcggccgac ctcgagcatg tagtcctcgg cctcgccgcc
      661 ctcgagcggc tcgcccgtgg tcatcctctc gccgcggaag tcaatgatga cgaagtcgcc
      721 gtcccccacg ggccggtcct cgaccgccgc gagcgtcgcg aactgtccgc gcatctgctc
      781 gagctgctcg tccacctgct cttccgacac ctcggcctcg cgtctgggca cctcgacgcc
      841 cctgtactcg ccgagcgatg cctcgggccg gacggccacc gtggccgaga acttgaagcc
      901 ttcctcctcg tccaacggct cgtcgaagtg gatctcgggc ctgtctatgg gcctcaggtc
      961 cgtctcgacg accgcttccg agtaccaggt cggcagccgc tcctggagag cctcgtagta
     1021 gatgtagtcg cggccaaggt ggttctcgat aacgcgccgc ggcgccttgc caggcctgaa
     1081 gccgggtacc ctgacctgcc gacccagctc gcgcaccttg gcttccaccc ccgcccggac
     1141 cgcctcgggg ggcacctcga cgtcgaggcg aaccttgttg tcctcgagct tcgttacgtt
     1201 agccgccata cactcctagt tctatcttcg tcggcgcgcg accggcacaa caagcgaggt
     1261 gcgagaggcg ggatttgaac ccgcacgccc gaaggcacta gatcctaaat ctagcgtgtc
     1321 taccattcca ccactctcgc tctcgccgaa acctgtcgcg attataacag ccgcgttaat
     1381 cgcgcccgcc cctccaaagg ccctttacgc tacgggcgca aggttacaac gccgcgcccg
     1441 aaccacgcga agctcggggg cgatttcgcc ccaagacgcg ggcattcccg agctccctag
     1501 tagcgaaatt gcgccaggat ggcttcgttg gcctcctggt ccggcacgag gacctcttcc
     1561 ccgttcggca aggtcttcgg cgttcctttg agctgggagg cggtcatctg ggcgtgttcg
     1621 cgacttatca gaaggcggcc aagcgagagc gcctcgtcga aatcgaggtt ggtctcgacg
     1681 tttctgttca tgaccctcgc gatgtggggc agcttggtca cggtgtccca cccgaatgcc
     1741 ttgctgcgca gggcggcgat caggcgctgt tgatgctcta tgcggtccat atctcccccc
     1801 gccgtgcccc tgtagcgagc gtagaagagc gcctggtggc cgttaagggt ctgaatccca
     1861 tcctttatgc cgtacttcgg cgggatctcg tcctccacgt ctatctccac cccgcccatg
     1921 gcgttgatga cgtcacggaa cccctcgaag tcaacgacgg cgtaatgatc tattggcact
     1981 tgggcgtagc cttgcagcgc ccccatggtc tcctctatac cgccgtagga gtaggcggcg
     2041 ttgat
//
LOCUS       DASTUV010000322         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1050349, whole genome shotgun sequence.
ACCESSION   DASTUV010000322 DASTUV010000000
VERSION     DASTUV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050349"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1374
                     /locus_tag="VFT03_06465"
     CDS             157..1374
                     /locus_tag="VFT03_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU4847822.1"
                     /translation="MSDRPLVIKFGGTSVGSGAAFARAAAIAAEAARERSVAVVVSAM
                     SGTTDTLLGYADATGGRKGRTLTGATREGSVAELHRSLAERHLRAAREVVSPEHLPAV
                     EERLRALLIELTRAFDAPAGNPAARRAGIAVHGERLSTEILAGALKSLDVPAAAVAHD
                     PIVTDAAFDGAEVDAAETRARCSRFVAPLLEGGTVAVVPGYVGRSPEGLATTLGRGGS
                     DLSATVLGRALGAEEVWIMSDVDGVLDADPRLVPDASRLPRLSYREASLFAALGAKVL
                     HPKTMEPASEAGIEVLVRNTFNPESPGTRVSGLAPEEGIRCLALRRRFKAEIPCAPGH
                     KREAAAVICIGSPARGDLERGQRCLREGGIAPLHAEDGTAGLVFIVAADVAEEALRVL
                     HGGLLSPAEEAVA"
     gene            1371..2456
                     /locus_tag="VFT03_06470"
     CDS             1371..2456
                     /locus_tag="VFT03_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847823.1"
                     /translation="MKRLEIVQIGLGHVGRAVAQIVLEERKHWRERYGLELVYRAVSD
                     TSGALVGEALLPQAIRTKEDGGKLSELGVEPLEEILVSEPAPGYTRVVVDVAVHGGTY
                     DLDLLGIQNGSCLVLSNKGPLSESTERYEKLTRMLPDRLWHEATVGAGMPIISTLDGL
                     LASGDEILEIQASPSGTLGFVMSAVEAGRRFSEAVKEAVALAYAEPDPRDDLSGLDVA
                     RKAIILARKMGRTIEPEEIPYRSLVPEGLEDVSLEEFMERLPEHDEAFAERLLAVEEH
                     HMLRYLARIPREGPVTVGLVDEPVESPFGPISGPENVFDFRTRRYSDVTLTIRGPGAG
                     PERTGSGVVFDLLDIAHKVVKDDREEL"
     gene            2458..>3168
                     /locus_tag="VFT03_06475"
     CDS             2458..>3168
                     /locus_tag="VFT03_06475"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU4847824.1"
                     /translation="MREGYTVAVVGAGLVGERLVAELRRREFPLKELRVLARSARRAV
                     LAGETFEVGVAEPEAFEGVDFAFFAGTEGEKGAALQLAQEAIARGAIVIDNGSDFRLD
                     PEVPLVVPEVNPGALKDHKGLIANANCSTIQMVVTLAPLIREFGIQKVVVSTYQAVSG
                     SGRSGVEALENGRADVYPKPIAGNAIPLIGSVGEDGLTTEETKMREESRKIMGLPDLE
                     VHATAVRIPVHTGHSESVY"
BASE COUNT          531 a         1068 c         1086 g          483 t
ORIGIN      
        1 tcatacaccc ggcttcgacc acccattccc agctctccgt tgacgagcag atgtcgaccg
       61 gggtcacccc tgactacgtc cggctctcgg tcggtctcga gtccatcgac gacatcctgg
      121 gcgatcttga ccaggcacta agtggagcgt gacggcatga gcgacagacc cctcgttatc
      181 aagttcgggg gcacctcggt cgggagtggc gcggcattcg cccgcgccgc cgcgatcgcc
      241 gccgaggccg cccgggagcg ctctgtggcc gtcgtcgtct cggcgatgag cggtaccacc
      301 gatactctgc tcggctacgc cgacgccacc ggcgggcgca agggccgaac cctgaccggc
      361 gcgacgcgcg aggggtccgt cgccgagctc caccgctcgc tcgcggagag gcacctgcgg
      421 gcggcccgcg aggtagtctc ccccgagcac ctccccgcgg tcgaggaacg tctgcgggcc
      481 ctgctcatcg agctcacgag ggccttcgac gcgccggccg ggaacccggc cgccaggcgc
      541 gccgggatcg ccgtccacgg tgagcggctg tctacggaga tcctggccgg agccctaaag
      601 agcctggatg tgcctgccgc cgccgtagcg cacgacccga tagtcaccga tgccgccttc
      661 gacggggcgg aggtagacgc cgccgagacg cgggcgcgct gttcgcgctt cgtagcgccg
      721 ctactcgaag gaggtaccgt cgcggtcgtg cccggctacg tcgggcgctc acccgagggg
      781 ctggcgacca cccttggcag gggcggctcg gacctctccg ccacggtcct cgggcgggct
      841 ctgggggccg aagaggtctg gatcatgtcc gacgtggacg gcgtgctgga cgccgacccc
      901 aggctggtgc ctgacgcctc caggctgccc cggctctcgt acagggaggc aagcctgttc
      961 gccgcgctcg gcgccaaggt cctgcacccg aagacgatgg agccggcctc agaggctggt
     1021 atcgaggtcc tggtgcgcaa caccttcaac cccgagagcc cgggtactcg cgtgtccggc
     1081 ctcgcgccgg aagagggtat tcgctgcctc gcgctgcgcc ggcgcttcaa ggccgagatc
     1141 ccctgcgccc cgggccacaa gcgggaggct gcggctgtga tctgcatcgg gtcgcctgcc
     1201 cgcggtgacc tcgagcgtgg ccagaggtgc ctgcgcgagg gggggatagc gccgctgcac
     1261 gccgaggacg gcaccgccgg gctcgtgttc atcgtcgccg ccgacgtggc cgaagaggcg
     1321 ctgcgcgtcc tgcacggggg tctgctctcg cccgcagaag aggcggtcgc gtgaagcggc
     1381 tcgagatagt tcagatcggg ctcggccacg tcggacgggc tgtcgcccag atcgtgctgg
     1441 aggagcgcaa gcactggcgc gagcgttacg gcctcgagtt agtctacagg gccgtctccg
     1501 atacttcggg cgcgctcgtg ggcgaggccc tgctgccaca ggcgatacgg accaaagagg
     1561 acggcggcaa gctctccgag ctcggcgtcg agcccctgga ggaaatcctc gtatccgagc
     1621 ctgcgccagg gtacaccaga gtagtagttg acgtcgcggt gcacggcggt acctacgatc
     1681 tagacctgct cggaatccag aacggctctt gcctggtgct ctccaacaag ggcccgctct
     1741 cggagagcac cgagcgctac gagaagctca cgaggatgct gccggacagg ctctggcacg
     1801 aagccacggt cggagccggg atgcccataa tctcgaccct cgacggactg ctcgcgagcg
     1861 gagacgagat actcgagatc caggctagcc cgagcggcac tctgggattc gtaatgagcg
     1921 ccgtcgaggc cggccgccgc ttctccgagg ccgtcaaaga agcggtcgcc ctcgcctacg
     1981 ccgagcccga cccgcgcgac gacctctccg gtctcgacgt cgcgcgcaag gctataatcc
     2041 tggcccgcaa gatgggccgc acgatagagc ccgaggagat accttacaga tccctcgtcc
     2101 ccgaaggtct cgaagacgtc tctctggagg agttcatgga gagattgcct gagcacgatg
     2161 aggcgttcgc cgagcggctg ctcgccgtcg aggagcacca catgctccgc tacctcgccc
     2221 gcatccccag ggaggggccg gtgaccgtgg ggctggtgga cgagcccgtc gagagcccct
     2281 tcggccctat aagcggcccc gagaacgtct tcgacttccg aacgcgccgc tactctgatg
     2341 ttaccctcac gatacgcggc cccggcgcgg ggccagagcg caccgggagc ggcgtcgtct
     2401 tcgacctgct cgacatagcg cacaaggttg tcaaggacga tagagaggag ctctgatatg
     2461 cgcgagggat acaccgtggc ggtcgtggga gctgggctcg tcggcgagcg cctggtagcg
     2521 gagctcaggc gccgggagtt tcccttgaag gagctgagag ttctcgctcg cagcgcccgc
     2581 agggctgtcc tcgctggaga gactttcgag gtaggggtag cagagccaga ggcattcgag
     2641 ggcgttgact tcgccttctt cgccgggacg gagggtgaga agggcgcagc cctgcagctc
     2701 gcgcaggagg caatagctcg gggtgctatc gtcatagaca acggcagcga cttccggctc
     2761 gaccccgagg tgccgctcgt cgtgccagag gtgaacccgg gcgccttaaa ggaccacaag
     2821 ggcctcatcg ccaacgccaa ctgctcgacc atacagatgg tcgtcaccct cgcaccgcta
     2881 attcgagagt tcggcatcca gaaggtcgtc gtctcgacct accaggcagt ctccggctcg
     2941 ggacgctcgg gagtcgaggc cctggagaac ggcagggcgg acgtctaccc caagcccata
     3001 gctgggaacg ccatcccgct cataggcagt gtgggggagg acggcctcac gaccgaggag
     3061 accaagatgc gagaggagtc gcgcaagatc atgggtctcc ccgacctgga agtgcacgcg
     3121 accgccgtgc gcatccccgt ccacaccggt cactccgaga gcgtctac
//
LOCUS       DASTUV010000323         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_709709, whole genome shotgun sequence.
ACCESSION   DASTUV010000323 DASTUV010000000
VERSION     DASTUV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709709"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFT03_06480"
     CDS             <1..665
                     /locus_tag="VFT03_06480"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU4847825.1"
                     /translation="NAWVMKAINLYFAVAEMETIEVGPFEYHDKIPTKKKLAEAGVRV
                     VPPGTIRYGAFVEPGVVVMPGYVNIGAYVGSGTMVDTWATVGSGAQIGRNVHLAGGVG
                     IGGVLEPPGAMPVVIEDGAFVGSRAVVVEGVRVEEEAVLGANVVLTSSTQIIDVTGEE
                     EAVYRGRVPARSVVVAGTRTKEFPAGCYQLQTALIIGKRRESTDQKTSLNEALRQFGV
                     SV"
     gene            662..1726
                     /gene="dapE"
                     /locus_tag="VFT03_06485"
     CDS             662..1726
                     /gene="dapE"
                     /locus_tag="VFT03_06485"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEU4847826.1"
                     /translation="MRERLLEGLLWFLERPSVTGEEKPLCDDLEARVSRLAGWETQRV
                     ANNLVVYRAEPDGSRTRLVFAGHLDTVPVPQGGLSVRVEDGRVYGRGASDMKAGDAVM
                     LALVEDFSWEDSWAEPLFVFYEREEGPYAENGLELVFAEIPDVLDADLTLVLEPTAGA
                     LEVGCVGTAQVEVTFKGKSAHSARPWQGNNAITAAASFLAEMHERQVEEVVVEGLTFY
                     EVLVPTLARGGRAKNVVPDEFWINVNYRFSPSKGIDDVRSVFEGLLRGDARFDIVDYA
                     PSGPVELDNPLLRRLIEAELEVRPKQAWTDVARFAQRGVAAANFGPGLPAQAHQESEY
                     AEIPLLERCYERLASFLMHN"
     gene            complement(1770..2930)
                     /locus_tag="VFT03_06490"
     CDS             complement(1770..2930)
                     /locus_tag="VFT03_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4847827.1"
                     /translation="MKLDRWSVRADKTGRRGMGRRTRTPTPIFVSPRVAVVLSVVGAA
                     ALAYVLYAAPSILVVALGGTALAVVLSFPVRALSHVMPRGPAILLTFLGLLGMVGLAL
                     GYLVPLLVQQMSRLIVRTPDIANSANRLLIDAIAFLDRRELVPGSDPEAFGRELVTDL
                     FDRAQSVTENLLRDLIVFIPQVFNFGIILFAVLFVAAYLLVDVRKVKAVYLKAAPKRY
                     RQDARDLWDAFGYSLSRYLGGLIFVAAVQGVLAGVALWILDVPFALLLGVWISITAII
                     PYLGAFLGAIPAVVLAFFQSPTTGVLAVVAYILIQQLEGNFLTPRIQGQVLHVHPILV
                     LLAVIGGGQLAGLAGVIFAVPTLAVLRVFFDFFRARIRTSPDAGPEGPALPD"
BASE COUNT          567 a          943 c         1104 g          470 t
ORIGIN      
        1 cgaacgcgtg ggtgatgaag gcgatcaacc tgtacttcgc ggtggccgag atggagacga
       61 tcgaggttgg gccgttcgag taccacgaca agatccctac caagaagaaa ctggccgaag
      121 cgggcgtgcg cgtcgtaccc cccggcacta tccgctacgg agcgttcgtc gagccgggcg
      181 tagtcgtgat gccagggtat gtgaacatcg gagcctatgt ggggagcggg acgatggtgg
      241 acacctgggc gacggtcggc tccggggccc agatcgggcg caacgtgcac ctggctggag
      301 gcgtgggcat cggcggcgtc ctcgagccgc cgggggcgat gccggtcgtg atcgaagacg
      361 gagccttcgt cggctcgcgg gccgtcgtcg tcgagggggt tcgcgtcgag gaggaggcgg
      421 tcctcggggc gaacgtggtc ctaacgagct cgacacagat catcgacgtc acgggcgaag
      481 aagaagccgt ctaccgcggc cgcgtcccgg cacgctccgt cgtcgtcgcc ggcacacgaa
      541 ccaaggaatt tccggccggc tgctaccagc tccagacagc cctcatcatc ggcaagcggc
      601 gggagtccac cgaccagaag acctccctga acgaggccct ccggcagttt ggggttagcg
      661 tttgagggag cgcctgctcg agggcctgct ctggttcctc gagcgcccga gcgtcacggg
      721 cgaagagaag cccctctgcg acgacctgga ggcgcgcgtg tcgcggttgg ccggctggga
      781 gacgcagcgc gtcgcgaaca acctcgtcgt ctacagggcg gagcccgacg gttcgagaac
      841 tcgtctcgtc ttcgccggac acctcgatac tgtgccggtg ccacagggcg gtctgtctgt
      901 gcgcgtcgag gacgggaggg tctacggtcg cggcgcctcg gacatgaagg ccggagacgc
      961 cgtgatgctc gcgctggttg aagacttctc gtgggaagac tcatgggctg agcccctctt
     1021 cgtcttctac gagcgggagg aaggacccta cgcggagaac ggcctcgagc tcgtcttcgc
     1081 ggagattcca gatgtactcg acgcggacct caccctggta ctggagccga cggcgggggc
     1141 actcgaggtc gggtgcgtcg gcacggcgca ggtcgaggtt accttcaagg gcaaatccgc
     1201 ccactccgcc cggccgtggc aggggaataa cgccattacg gcggctgcct cttttctggc
     1261 ggagatgcac gaaaggcagg tggaagaggt cgtcgtggag ggactgacct tctacgaggt
     1321 gctagtgccg acgctcgcac gaggcgggcg ggccaagaac gtcgtcccag acgagttctg
     1381 gatcaacgtc aactaccgtt tctcgccaag caaaggtatc gacgacgtgc gcagcgtctt
     1441 cgagggacta ctcagaggag atgcccgctt cgatattgtg gactacgctc cgagcggacc
     1501 cgtcgagctg gacaacccgc tgctgcgacg gcttatcgag gcggagctgg aggtgcgccc
     1561 caagcaggcg tggacggacg tggcccgctt cgctcagcgg ggagtggcgg cggcgaactt
     1621 cggacctggc ctgccggcgc aggcgcacca ggagtccgaa tacgcggaga tcccgctgct
     1681 cgagaggtgc tacgagcgcc tggcctcttt cctgatgcat aactagggac cgaagagcca
     1741 gcggggaagg cgcagcagat cgtcgagggt cagtcgggca gggccgggcc ttcgggacct
     1801 gcgtcggggc tcgtcctgat tcgggcccgg aagaagtcga aaaagacgcg cagtacggcg
     1861 agggtcggga cggcgaagat cacgccagcc agccctgcga gctggccgcc gccgatcact
     1921 gccagtagca cgaggatcgg gtgcacgtgc aggacctggc cctggatgcg cggtgtgagg
     1981 aagttgcctt cgagctgctg gatcaggata tatgcgacca cggcgagcac gccggtcgtc
     2041 ggcgactgga agaaggcgag cacgaccgcc ggtatcgccc cgaggaaggc cccgaggtag
     2101 ggaattatcg ccgtgatgga tatccacacg ccgagtaaca gggcgaaggg cacgtcgagg
     2161 atccagagcg ccacgccggc gagcacgccc tggacggcgg ccacgaagat gagaccgccg
     2221 aggtagcgcg agagcgagta cccgaaggcg tcccacaggt ccctggcgtc ctgcctgtag
     2281 cgtttcgggg ccgccttgag gtagaccgcc ttgaccttgc gaacgtccac cagcaggtac
     2341 gccgccacga agagcaccgc gaagaggatt atgccgaagt tgaacacctg ggggataaag
     2401 acgatcaggt ccctgagcag gttttcggtg acgctctggg cgcggtcgaa gaggtcggta
     2461 acaagctccc tgccgaacgc ctcggggtcc gaaccgggca ccagctcgcg cctgtcgagg
     2521 aacgctatgg cgtcgatgag gagcctgttg gcgctgttcg cgatgtccgg cgtccggact
     2581 atcaggcggc tcatctgctg cacgagcagc ggcaccagat aaccgagcgc gagccccacc
     2641 atcccgagaa ggccgaggaa ggtcaggagt atagcagggc cgcgcggcat gacgtgcgag
     2701 agggcacgca ccgggaaaga cagcaccacg gcgagggccg tgcccccgag cgctacgacg
     2761 aggatgctgg gggcggcgta caggacgtag gcgagcgccg cggccccgac caccgagaga
     2821 acaacggcga cgcgcggcga gacgaagatg ggcgtggggg ttctcgtccg cctccccata
     2881 ccccgccggc cggtcttgtc ggcccggacg gaccacctgt ctagcttcat cgccccttgc
     2941 cttctcgatg cataacctga ccatattcta cgccgaaggc tgccgggacg gtgcgcatgc
     3001 tgctcgttga gcggcttcta cgcgccgatc aggtgcgcgc cgccgttgct gcgggccagc
     3061 tcgagcgtcc gctcatcgag gggg
//
LOCUS       DASTUV010000324         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_469912, whole genome shotgun sequence.
ACCESSION   DASTUV010000324 DASTUV010000000
VERSION     DASTUV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469912"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFT03_06495"
     CDS             complement(<1..370)
                     /locus_tag="VFT03_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU4847828.1"
                     /translation="MRLDGKKIVVLVAEGFEDLEYWVTVMRLREEGAEVISVGTSLDP
                     VYGKNALQAQPDATAEEVDASELDGIVIPGGWAPDKLRRYPEITGLVRAVYQAGRTVG
                     IICHGGLVAISAGIVDGVRAT"
     gene            complement(435..>2238)
                     /locus_tag="VFT03_06500"
     CDS             complement(435..>2238)
                     /locus_tag="VFT03_06500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEU4847829.1"
                     /translation="RMLYVAPERLRSLEFVLSLRRAGVGLFVVDEAHCISEWGHDFRP
                     DYLFLPRAVRDLGSPPVLALTATATPRVREDILRSLKMRSPEVVVTSFNRPNLTYRVL
                     SAKEKKHKLPLILDVIRNAAPPGIVYATTRKECEELAAHLRRSGVDAAAYHAGMGPAE
                     RSDVQERFMTDELSVVVATIAFGMGVDKPNVRFVVHSSVPGSLPAYMQESGRAGRDGE
                     SSECVILYRSADLGRRKRLVTLGTSGEEEVGSLFHALAGLERDGRVNVQPSALPALGG
                     VDPDSAWIVLGSLEESGLIHRGYDLWAEVEVRRLEEEREGLREEVAAVHAALPGGGTI
                     SLPELSLRAGVRPAVAQGALYRMMVDGVAEIIPRGSVVDVRLKASVLDARSRRIITGR
                     LKSRAKAAYAQIRNVEAYTNLSSCRREHLLRHFGDTEPVAPCGACDVCLGEAEIPGSE
                     PPRAAPETLLSGTAVRKDGSISPELFERLKRWRGEQARRQQVPAYVVLHNSHLEEIAA
                     RKPQTIHELGSIKGIGLRRAARYGEDLLALVRGEEAPDQDGDTAGYRPHLEAADRLIR
                     SGQGAEAVSELARALELGGDAAKEAVNELLKPTS"
BASE COUNT          335 a          790 c          735 g          378 t
ORIGIN      
        1 ccgtcgccct gacgccgtcc actatcccgg ctgagatcgc gacgagcccg ccgtggcaga
       61 taatgccgac ggtcctgccg gcctggtaca ccgccctgac gaggccggtg atctccgggt
      121 agcgtcgtag cttgtccggt gcccagccgc cgggtatgac tataccgtcg agctcggagg
      181 catccacctc ctccgccgtc gcatccggtt gggcctgcaa ggcgttcttg ccgtagaccg
      241 ggtcaaggct cgtcccgacc gagatgacct ccgccccctc ctcccgcagc cgcatcaccg
      301 tgacccagta ctccaggtct tcgaatccct cggctaccag caccacgatc ttcttcccgt
      361 caagcctcat ccccatctcc tttctattag catttcagca cgctacgccc ttttagggct
      421 tcgctttcag caattcagct agtcggcttc aggagttcgt ttacggcctc ctttgctgcg
      481 tcgcctccga gctccagggc gcgagccagt tcggagacgg cctcggcgcc ctgtccggaa
      541 cgaatgaggc ggtcggcggc ttccagatgt gggcggtagc ccgccgtgtc accgtcctga
      601 tcaggcgcct cctcaccgcg gacgagcgcg agcagatcct cgccgtagcg ggcggcgcgt
      661 ctgaggccga taccctttat ggagccgagc tcgtggatgg tctgcggctt gcgggccgct
      721 atctcctcca ggtgcgagtt gtggagcacc acgtatgcgg gaacctgttg ccgccgcgcc
      781 tgctcccccc tccagcgctt gagccgctcg aagagctccg gggaaatcga tccgtccttc
      841 cgcacggcgg tcccactcaa gagcgtctcg ggcgcagcgc gcggcggctc gctaccgggt
      901 atctcggctt cgccgaggca cacgtcgcag gcgccacagg gcgccaccgg ctccgtgtca
      961 ccgaagtgcc ttaggaggtg ctcgcggcgg cagctgctca ggttcgtgta ggcctctaca
     1021 ttccggatct gggcgtaggc cgctttcgcg cggctcttga gcctgcccgt gatgatgcgg
     1081 cggctgcgcg cgtcgagcac cgaagccttg agcctcacgt ccacgacgga cccgcgaggg
     1141 atgatctccg ccactccgtc taccatcatc cgatacaggg cgccctgcgc tacggcgggg
     1201 cgaacgcccg cgcggaggga gagctcggga aggctaatgg tgcctccgcc gggcagcgcc
     1261 gcgtgcacgg ctgccacttc ctcgcgcagc ccctcccgct cctcttccag acggcgcacc
     1321 tcaacctcgg cccagaggtc gtagccgcgg tggatcagcc ccgactcctc caggctgcca
     1381 aggacgatcc aggccgagtc cgggtccacc cccccgagcg cgggaagggc gctgggctgg
     1441 acgttgaccc tcccgtcacg ctcgagccct gcgagcgcgt ggaagagcga accgacttcc
     1501 tcttcacccg aggtacctag cgtcaccagc cgcttgcgac gcccgaggtc ggcgctgcgg
     1561 tagaggatga cgcactccga gctctctccg tcgcgcccgg cgcggcctga ctcctgcatg
     1621 tacgcgggca ggctccccgg gacgctcgag tggaccacga accggacatt cggcttgtcc
     1681 acccccatcc cgaaggctat cgtggcgacg accacggaga gctcgtccgt catgaagcgc
     1741 tcctgcacgt ccgagcgctc cgcaggcccc atacccgcgt ggtaggcggc ggcgtccaca
     1801 ccggagcggc gcaggtgggc ggccagctcc tcgcactcct tgcgggtcgt cgcgtacacg
     1861 atgccgggag gcgcggcgtt gcgtattacg tccaggatca ggggcagctt gtgcttcttc
     1921 tccttcgcgg agaggaccct gtaggtgagg ttcgggcggt tgaagctcgt aacgacgact
     1981 tcgggcgagc gcatcttgag cgagcggaga atgtcctcgc ggacgcgcgg ggtagcggtg
     2041 gcggtgaggg cgaggacggg cgggctaccc aggtccctca cggcgcgggg caggaagagg
     2101 tagtcggggc ggaagtcgtg gccccattcg gagatgcagt gggcctcatc cacgacgaag
     2161 aggccgacgc ccgcgcgccg cagcgagagg acgaactcga gcgagcgcag gcgctccggg
     2221 gcgacgtaga gcatccgg
//
LOCUS       DASTUV010000325         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_31250, whole genome shotgun sequence.
ACCESSION   DASTUV010000325 DASTUV010000000
VERSION     DASTUV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31250"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VFT03_06505"
     CDS             complement(<1..1188)
                     /locus_tag="VFT03_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847830.1"
                     /translation="MSEEPRDQVDELARGIEAAGALIESLEEEVANLRRDLEQASVAL
                     KAAQEEVSARGRALEEQERARAEAERKVGNLRAELSELRNRHSDEQLRLRNQHINELA
                     DARRELEEQRRVEVEAASSEERVSTLKEEFRREREALEQRHEAEIEALKTSSQQWEEK
                     LREGYREQEERHAAELESMRLEVEERKEELELRLREDFQRRYAEERRAADERQEAALH
                     ALRNAAAGRELELQRDYQAVVETQQAEIESLREQVEQQAREAKEARQKELRKVKSQAE
                     SRERELRRTQASKLSETSDTAERRVASLQAQREADNRALRARHTEEIARLRREYEERL
                     AAEDERRKLETWALEERLGEARIQRETEVRAYTARLDELEAARLSQKTSAEEDLERVV
                     ERFG"
     gene            complement(1383..1829)
                     /locus_tag="VFT03_06510"
     CDS             complement(1383..1829)
                     /locus_tag="VFT03_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU4847831.1"
                     /translation="MDTIVEMPGVLEPDIREKFRRSGYTLTTQRRAVLDALNEAGGHP
                     SAEDIYLIVKRKNPRVALGTVYQALSVLEEIGVIGSKHWSESPTRYDLNLEPHLDIRC
                     VQCGAVSEIPGIELGELEESIRRNTPYEVTRTTIVVEGLCPSCKGT"
     gene            1900..>2797
                     /locus_tag="VFT03_06515"
     CDS             1900..>2797
                     /locus_tag="VFT03_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEU4847832.1"
                     /translation="MSVLTDVLVNLWRLLRNAYVRLLRRPPEYVSLEVAGSLPEFESR
                     VGFIRRRMRPGPTGPSLEGLRGILARIAGDGRASGVVLRIRSLDAGWAALEELRREIT
                     AFRERGGRVVAYLLDPVDSRSYYLACAADEIVVTPLATIGVTGLRTSVNFLKEALNRI
                     GVEAEVVAVSPYKSAGDPFVRNDFSRESREQAERLLDRRFEELVGAIARGRDLSLEEA
                     RSAIDGAPYSAPEGLSRGLLDGVCYEDELPERLGTEGSRAKLVEWAAARRSLRVPYRR
                     SPRRRVGLVSLSGTIVRGKSRRL"
BASE COUNT          380 a          955 c          886 g          576 t
ORIGIN      
        1 cccgaatcgc tcgaccacgc gctcgaggtc ctcttctgcc gaggtctttt gtgagagcct
       61 tgcggcctcg agctcgtcca ggcgggcggt ataagcgcgg acctcggtct cgcgctggat
      121 cctggcttcc ccgagccgct cctcgagcgc ccaggtctcc agcttgcggc gctcgtcctc
      181 ggccgcgagc cgctcctcgt actcgcgtct caggcgcgca atctcctcgg tgtggcgtgc
      241 ccgtagcgcc ctgttgtccg cctctctctg ggcctggagg gaggcgaccc ggcgctcggc
      301 ggtatcgctc gtctcagaga gcttggatgc ctgcgtgcgc cgcagctcgc gttccctgct
      361 ctcggcctgc gacttgacct tgcgcagctc tttctgccgc gcttctttag cctctctggc
      421 ctgttgttcc acctgttccc gcagggactc tatctcggct tgctgggtct cgaccaccgc
      481 ctggtaatct ctctggagct caagctctct gccggccgcg gcgttcctga gcgcgtgcag
      541 cgccgcctcc tggcgctcgt cagccgcgcg ccgctcctcg gcgtagcgcc gctggaagtc
      601 ctccctcagg cgaagctcca gctcctcctt ccgctcctcg acctccagcc gcatgctctc
      661 gagctcggct gcgtgacgct cttcctgctc gcggtatccc tcccgcagct tctcttccca
      721 ctgctgtgag gaggtcttga gcgcctcaat ctccgcctcg tggcgctgct ccagcgcctc
      781 ccgctctcgc ctgaactcct ctttgagcgt cgatacgcgc tcctcagagg aagcagcctc
      841 cacttccacg cggcgctgct cctcaagttc gcggcgagca tcggcgagct cgtttatgtg
      901 ctggtttcgg agcctgagct gttcgtctga gtgacggtta cggagctcgg agagctcggc
      961 gcggaggttc ccgaccttcc gctcagcctc ggcgcgggcg cgctcctgct cctcgagggc
     1021 tcggcctctg gccgagacct cctcctgggc ggctttgagc gcgacgctcg cctgctccag
     1081 gtcgcggcgg aggttcgcta cctcctcctc gaggctctcg atcaaggctc ccgccgcctc
     1141 aatccctctg gcgagctcgt ctacctgatc cctcggttcc tcgctcacct cagccctttc
     1201 tggggagttc gccgcgcaac ccgttcctcc tggaatcgcg tgtggtttag agtaaacctt
     1261 gacgcgcttc tggtaaaggg catcgtgacg agcggggccc gctcctcgca cctgccgtgc
     1321 aggcaaatat gggagttgca ttgagcgctg ccggatgagt ggaccctcac gggcccgtga
     1381 gatcaggtcc ccttacagga ggggcagaga ccctcgacga cgatcgttgt gcgggttacc
     1441 tcgtatggag tgttgcgcct gatgctctct tccagctccc caagttcgat gccggggatc
     1501 tcggacaccg cgccgcactg aacgcaccgg atgtccaggt gcggctcgag gttcaggtcg
     1561 tatcgcgtgg gcgactccga ccagtgcttg gagccgatca ccccgatctc ctcgagcacg
     1621 gagagcgcct ggtagacggt gcctaaggct acgcgcgggt tcttcctctt gacgatgagg
     1681 tagatgtctt cggctgaagg atgccccccg gcctcgttga gcgcgtcgag cacggcccga
     1741 cgctgagtgg taagagtgta ccccgaccgt cggaacttct cccggatatc tggctccagc
     1801 acgccgggca tctctacgat ggtgtccaat cagtaaccat tcctattccg gagtctatca
     1861 tacggcatcc accctgcggc ggtgtaacat tagtccagga tgtccgtcct gaccgacgta
     1921 ctggtgaacc tgtggcgcct gttgcgcaac gcctacgtgc gcctcctgag gcggccgccc
     1981 gaatacgtct ctttggaggt ggccgggtcc ttgcctgagt tcgagtccag ggtcggcttc
     2041 atccggcgtc gtatgagacc tggcccaacc ggtccgagtc tggaggggct acggggtatt
     2101 ctcgccagga tcgccggcga cggccgtgcc agcggagtcg tattgcgcat ccggagtttg
     2161 gacgccgggt gggccgcgct ggaggagttg cgccgggaga tcaccgcttt cagagagcgc
     2221 ggcggccggg tagtagcgta cctcctggac cccgtggaca gccgttcgta ctatctggct
     2281 tgcgcggcag acgagatcgt ggtcaccccg ctcgcgacca tcggcgtcac cgggctccga
     2341 accagtgtca acttcctcaa ggaagccctg aaccgtatag gagtcgaggc cgaggtagtc
     2401 gcggtctcgc cgtacaagtc ggctggggac cccttcgtcc gcaacgactt ctccagggag
     2461 tcccgcgagc aggccgagcg cctcctcgac cgcaggttcg aggagctggt tggcgctatc
     2521 gcgcggggga gagacctctc tcttgaggag gcacgctccg ccatagatgg cgccccctac
     2581 tccgcacccg aggggctatc ccgcggcttg ctcgacggtg tgtgctacga ggacgagctg
     2641 ccggagaggc ttgggacaga agggtcacgc gcgaagctcg tggagtgggc cgcggcgcgc
     2701 cgttcgcttc gcgtaccgta ccgcaggagc ccgcgccggc gggtagggct cgtgagcctc
     2761 tcagggacta tcgtccgggg gaagagccga aggttgc
//
LOCUS       DASTUV010000326         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_437354, whole genome shotgun sequence.
ACCESSION   DASTUV010000326 DASTUV010000000
VERSION     DASTUV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437354"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1020)
                     /locus_tag="VFT03_06520"
     CDS             complement(154..1020)
                     /locus_tag="VFT03_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU4847833.1"
                     /translation="MSNQATHPREADPETPALLAGYFARIDTGTLLTHAEEVDLSRRA
                     RTGDKRARQRLIEKNLRLVVSVAKRYRDNGLPFEDLIQEGNIGLMKAVERFDPEKGFR
                     FSTYATWWIRQAVQRAVVDKGRTVRIPVHMSEKVRKTGKARNALSREHGREPTVEEVA
                     AHLGWEVEEVREALRATTHVSSLSRLVGPDGDTELGELVEDERVPDTPEAVIREMEAL
                     GLQTAVARLPQRTRHVLVRRYGLDDRKRATLEDLARELGISRERVRQIQLEAESILRT
                     RENRRMLREVVA"
     gene            1183..1866
                     /locus_tag="VFT03_06525"
     CDS             1183..1866
                     /locus_tag="VFT03_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4847834.1"
                     /translation="MKRILVVEDEERLARLISRVLREEGYAVETTGDGRTGLSRALSE
                     DFDLLILDWMLPDRDGLQIVRGLRAAEIRVPVLMLTARDQIEDRVEGLDAGADDYLSK
                     PFAFPELLARVRALTRRPWAEISDGMALSAGDVTLDPVRHEVRRGGERVELTAKEFAL
                     LATLLQRPGQVFTRSVLLDTVWGGSPEVYANVVDLYVSYLRKKLDHGGESSHIRTVRG
                     VGYTFDPQT"
     gene            1870..3117
                     /locus_tag="VFT03_06530"
     CDS             1870..3117
                     /locus_tag="VFT03_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4847835.1"
                     /translation="MLDRIRRRLTMGYVGILALILILFGCIVVLSFHRQVTVQQNRLL
                     EQRVESLAKVPYYGGGAHILEDPDKYAWVTLTPDGHVRSHNPTASSLGLPSEKLFREC
                     LSELEVVSGTVDRPVENVRVASSPIVQDGKVIGVLQVARSPAVVWGIVGSLVLVLVPI
                     GLGALLLSAVGGLYVSRRAMRPVQDAFERQRTFIADASHELKTPLTLIRADAEVLSSA
                     LTDPDDRELADDLISETDRMNALLSDLLTLARLDAGKLSVERKPFDLANTIAETAERF
                     DARAAAEGKRLEVRLDPADGIPARGDAGRTEQILASLLDNALRFTPPGGLVTVEGYPQ
                     DGKALATVTDTGPGIPHEHLPRVFDRFYRAEAARTRGDAGGGTGLGLSIALDLARAQG
                     GDLTAANAQPDGASFTLTLPAAS"
     gene            3212..>3772
                     /locus_tag="VFT03_06535"
     CDS             3212..>3772
                     /locus_tag="VFT03_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU4847836.1"
                     /translation="MRGDDLETRVEAIRRFNRLFTRRIGVLREGLLHSSYSLTEARVL
                     FELANREELSASELVRELGLDPGYMSRTVGGLERQGLVEKVRSETDGRRRILSLTAEG
                     EEAFKVLDSRSREEVSEMLGGLSEEEQRRLLEAMRAIEGIFEKGLKFSEPFVLRPHEP
                     GDMGWVVHRHGVLYAAEYGWDERFEAL"
BASE COUNT          602 a         1319 c         1182 g          669 t
ORIGIN      
        1 cctgcgccac gggctcgtcg ccgggcacgt ggccaggggc cgccacggct gtcttggcaa
       61 ggccatcggg tccctgctcg gtcgcggagc ggtagacggc ccccgccgcg acgagcagcg
      121 cgagcaccgt cagcaggagc aataaccgcg agctcatgcc accacctccc ggagcatcct
      181 gcggttctct ctggtccgca gtatgctctc ggcctccagc tggatctgcc taacccgctc
      241 ccgcgagatg ccgagctctc tggcgagatc ctcgagggtc gccctcttgc ggtcgtcgag
      301 tccgtagcgc ctgacgagga cgtggcgcgt gcgctggggc agcctcgcta ccgccgtctg
      361 caaccccaga gcctccatct cccggatcac cgcctcaggc gtgtccggta cccgctcgtc
      421 ctcgacgagc tcccccagct cggtgtcccc gtcagggccg acgagcctgc tcaggctgga
      481 gacgtgcgtc gtcgctctca aggcctcccg tacctcctcg acttcccacc cgaggtgcgc
      541 cgcgacctct tccacggtcg gttcgcgacc gtgctccctc gagagcgcgt tccgcgcctt
      601 gccggtcttc cgcaccttct cgctcatgtg gaccgggata cgtacggttc tccccttgtc
      661 cacgactgcc cgctgcacag cctgcctgat ccaccacgtc gcgtaggtgg agaacctgaa
      721 gcctttctcg gggtcgaatc tctctacagc cttcatcaac cctatgtttc cctcctggat
      781 caaatcctcg aagggaagcc cgttgtccct gtacctcttg gcgacgctca caacaagcct
      841 caggttcttc tcgatgagac gctggcgggc tctcttgtcg ccggtgcggg cgcgcctgct
      901 gagatccacc tcctccgcgt gcgtgagcag ggtgcccgtg tctatccggg cgaagtaacc
      961 tgcgaggagc gcgggcgtct ccgggtctgc ttccctaggg tgcgtagctt ggttgctcat
     1021 gcgcctctct cctcgtctgc tagcacgacc ttcttctcct tcgcagcgtc ctggccgtgc
     1081 ccaattctgg acccccagcc tgtgaggacg ctttgaggaa gatgagagtt tgcacagaaa
     1141 acacaccggt gtggcccaac ctctttagaa tgggacgaga agatgaagag gatcctggta
     1201 gttgaggacg aggagaggct ggcgcgtctg atctcccgcg tgttgcgcga ggagggctac
     1261 gcggtcgaga cgaccggcga cgggcgaacc gggctctcgc gcgccctctc cgaggacttc
     1321 gacctcctga tcctcgactg gatgctaccc gaccgcgacg ggctccagat cgtccggggc
     1381 ttgagggcgg cggagatcag ggtcccggtc ctgatgctca ccgcccgcga ccagatcgag
     1441 gaccgcgtcg aaggcctcga cgccggggcc gatgactacc tgtccaaacc gttcgctttc
     1501 cccgagctcc tcgcccgcgt gcgcgcgctc acgcgacggc cttgggccga aataagcgac
     1561 gggatggccc tctccgccgg cgacgtcacg ctggacccgg tgcgtcacga ggtccggcgc
     1621 gggggggagc gcgtcgagtt gacagccaag gagttcgccc tgctcgccac cctcttgcag
     1681 cgcccggggc aggtcttcac ccgctcggtc ctcctcgata ccgtctgggg cggctccccc
     1741 gaggtctacg ccaacgtcgt cgacctctac gtatcttacc tgcgcaagaa gctcgaccac
     1801 ggcggcgagt cctcccacat ccgcacggtc cgcggcgtgg gctatacttt cgacccccag
     1861 acctgaccta tgctggaccg catccgacgc cgcctcacga tgggctacgt gggcatcctc
     1921 gccctgatcc tcatcctctt cggctgcatc gtggtgctca gcttccaccg ccaggtcacc
     1981 gtgcagcaga acaggctcct cgagcagagg gtggagagcc tggctaaagt cccctactac
     2041 gggggcggcg ctcacatcct cgaagacccg gataagtacg cctgggtcac actgaccccc
     2101 gatgggcacg tcaggagcca caaccccacg gcttcgtccc ttggtcttcc atccgagaag
     2161 ctcttccgag agtgcctcag cgagctcgaa gtggtatccg gcacggtcga caggccagtt
     2221 gagaacgtgc gggtcgcgag ctcgcccatc gtgcaggatg ggaaggtgat cggcgtcttg
     2281 caggtcgcgc ggtctcctgc ggtggtctgg gggatagtag gtagcctcgt cctcgtcctc
     2341 gttcccatcg gcctcggcgc cctgctactg tccgcggtcg gagggctcta tgtgtcccgg
     2401 cgcgcgatgc gccccgtcca ggacgctttc gagaggcagc gcacgtttat agctgacgcc
     2461 tcgcacgagc tcaagacgcc gctcaccctg atccgggccg acgccgaggt cctctcctca
     2521 gccctcaccg accccgacga ccgtgagctc gcggacgacc tgatctccga gaccgaccgg
     2581 atgaacgccc tgctctccga tctcctgacc ctggcccgac tcgatgccgg gaagctctcc
     2641 gtcgagcgca agcccttcga cctggcgaac accatcgccg agaccgcgga acgcttcgac
     2701 gccagggccg ccgccgaagg gaagaggctc gaggtccgcc tcgatccagc ggacgggatc
     2761 ccggcccgcg gcgacgccgg gaggacggag cagatactgg cctcactcct cgataacgcc
     2821 ctccgtttta cgcccccggg cggcctcgtc accgtcgaag gctatcctca ggacggaaag
     2881 gccctcgcga ccgtgaccga taccggcccc ggcatacccc acgaacacct tccccgcgtc
     2941 ttcgaccgct tctaccgcgc cgaagccgcc cgcacccgcg gcgatgccgg cggagggacg
     3001 ggcctcggcc tctctatcgc cctcgacctc gcccgcgccc agggcggcga cctcacagcc
     3061 gccaacgcgc aaccagacgg agccagcttc accctcacgc tccccgccgc ttcctgatcc
     3121 ggccgtccgc agaccgccag attcctatct tgctagatac ttgactttag caagtaaata
     3181 atagacaata gcaaatgtac gaggaggtgg tgtgagagga gacgacctcg agacgagggt
     3241 cgaggcgatc aggcgcttca accgcttgtt cacgcgtcgg atcggggttt tgcgcgaggg
     3301 gcttttgcac agcagctact cgctcacgga ggcgagggtt ctattcgagc tggcgaaccg
     3361 cgaagagctg agtgcatccg agctggtgcg cgagctgggg ctggatcctg gatacatgag
     3421 tcgcaccgtg ggcgggctcg agaggcaggg gctcgtcgag aaggtccgtt cggagaccga
     3481 cggccggcgg cgcatactct cgctgactgc cgagggcgag gaggctttca aggtgctgga
     3541 tagccgctcg cgcgaggagg tatccgagat gcttggcggg ctctcggaag aggagcagcg
     3601 gcgtctgctc gaggcaatgc gggcgattga ggggatcttc gagaagggcc tcaagttctc
     3661 ggagcccttc gtcttgcgcc cgcacgagcc gggagacatg ggctgggtcg tccacaggca
     3721 cggcgtactc tacgccgcgg agtacggctg ggacgagcgc ttcgaggcgc tg
//
LOCUS       DASTUV010000327         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_831379, whole genome shotgun sequence.
ACCESSION   DASTUV010000327 DASTUV010000000
VERSION     DASTUV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831379"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFT03_06540"
     CDS             complement(<1..356)
                     /locus_tag="VFT03_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847837.1"
                     /translation="MERWGGANHPLVKAVAGFSVPLFLALNFPTIFLAILAGMLLYAI
                     GLLGVVHAYRRRYRIVGRPELALQLAAKSFSSLKENIGRTMTRGLANHQNRKRLQQER
                     KARLSRSRRERNRRKR"
     gene            603..1793
                     /locus_tag="VFT03_06545"
     CDS             603..1793
                     /locus_tag="VFT03_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU4847838.1"
                     /translation="MGIERAEYAARQAAARELARERGFSALVAWSRGGSTQDHYADVY
                     YLTGFYTHQPFIPDEPGHWRAHGHTALVLPVDGPATLLMDMTELQDPKPAADCVRYTE
                     DLVEALATTVAEKVPSGTDVALLGGEALAWRWGRELAARLPEHRFVEADALAAQLRLI
                     KSAAELDLLRAAGEVGTGAVTAAMEAAVPGATEAEVAAAAISEIVRAGGALYGLGLSS
                     GPWAHTFSPSTPAAYSRRRVSAGDMVRLDLYGSFDGYLFDFGRSRVVGRPPTQEQQEI
                     LDAVRDSVQAGTELLRPGETLGAVARLCEEVLESFAYTRRHGLPASMMGGTWGHSLGL
                     DWGSPWIEPTSSTVIRPGMCFAIERRIEAPGIGGANYENNVIVTHAGPENVTPAPDRY
                     GERP"
BASE COUNT          361 a          693 c          615 g          358 t
ORIGIN      
        1 tgacgtttcc tcctgttgcg ctcgcgtctg gatcgactga gtctcgcctt cctctcttgt
       61 tggaggcgct ttcggttttg gtggttcgcc agtccacgag tcatcgtccg gccgatgttc
      121 tccttgaggc tagaaaagga cttcgccgcg agctgtagtg caagctcggg tcttcctacg
      181 atccgatacc gcctcctgta ggcgtgcacg actcctagca atccgatcgc gtagagtagc
      241 atcccggcca ggatggctag gaagatggtc gggaagttca acgccaggaa cagggggacg
      301 gagaaccccg ccaccgcctt cactagtggg tggttagctc caccccaccg ttccatcggt
      361 ccccctcctt gacgaggtgc ttgcccgcat cctcagcagc cggcaaatac cgtgtcggta
      421 cccatttatg gtgcctagaa ggataatgca tcctagatgt agtggtcaac gcttgagttc
      481 agaatttgct cattgcgaca aaactcctct ccgagacagc tatactccac caggcagatg
      541 tcaccttggg agcgcagcgt catccgcgag tacgacacat caagacagta ataggaagta
      601 ctgtggggat cgagcgagcc gagtacgccg ctcgccaggc ggccgcccgc gagctcgcca
      661 gggagcgtgg cttctccgcc cttgtggcct ggtcccgtgg gggttccacc caggatcact
      721 acgccgacgt gtactacctc acgggcttct acactcacca acccttcatc cccgacgagc
      781 cagggcactg gcgcgcacac ggccacaccg ctctggttct cccagttgac ggaccggcga
      841 cgttgctaat ggacatgacc gagctgcagg atccaaagcc cgctgctgat tgtgtccgct
      901 acacggagga cctggtcgaa gcgctcgcga ctaccgtggc agagaaggtt ccctccggca
      961 ccgacgttgc gctgctcggc ggagaggcgc tcgcctggcg gtggggccgc gagctcgccg
     1021 cgcgccttcc ggagcaccgc ttcgtcgaag ccgacgctct cgccgcgcag ttgcgcctga
     1081 tcaagagcgc ggcggagctc gacctgctgc gtgccgcggg cgaggtcggt accggagcgg
     1141 taaccgccgc catggaggcc gccgtgccgg gtgcaaccga agccgaggta gctgcagcgg
     1201 ccatctccga gatcgtccgc gcgggcggag cactctacgg gctggggctc tcctcgggcc
     1261 catgggctca cacgttcagc ccctccaccc ccgcagccta cagccgacga cgcgtgagcg
     1321 ccggcgatat ggtccgcctc gacctctacg gatcgtttga cggctacctc ttcgacttcg
     1381 gccgctcacg cgtcgtcggg cggccgccaa cgcaagagca gcaggagatc ctcgacgccg
     1441 tccgagacag cgtccaggcg ggcacggagc tgttgcgacc cggcgagacg ctaggcgccg
     1501 tcgcccggct ctgcgaggag gtgctcgagt ccttcgccta cacccgccgt cacggcttgc
     1561 cggcctcaat gatgggcggc acttgggggc acagcctcgg gctcgactgg ggatcgccct
     1621 ggatcgagcc gacctcatcc accgtgatcc gaccgggcat gtgctttgct atcgagcggc
     1681 gcatcgaagc gcctggaatc ggcggcgcca actacgagaa caacgtgatc gtcacccacg
     1741 ccggccccga gaacgtcacc cccgcccccg accgctacgg cgagcggcca tgaggctcgt
     1801 cctgccacaa ccctccgacc ggtcggaggt aacatagcgt gatccacagc ctaatactat
     1861 tggtctgaca ctgatacgac cctcgaagag acggaggttg aagatatggc accagacccg
     1921 gacgagcgcg agccgctcga gcccgaagag cccacccctg acctcgggca ggctctctcc
     1981 accctctcca accgcgagtt gttgagcacg ctggacctta cgctact
//
LOCUS       DASTUV010000328         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1112522, whole genome shotgun sequence.
ACCESSION   DASTUV010000328 DASTUV010000000
VERSION     DASTUV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112522"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1071
                     /locus_tag="VFT03_06550"
     CDS             400..1071
                     /locus_tag="VFT03_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HEU4847839.1"
                     /translation="MKEESDTRLLALMVRTPDEPGMLHALTKVIFDHQANITYVDIAE
                     RREDGSTTYFELENVAAPDELIGDLESLPIVWAVEQAPSFQKVYGKRIIVVGGGAQVG
                     QVVVGAVAEADRHNIRGEKISIDTIPLVGEEELAAATRAVARLPRAVALVLAGALMGG
                     EVARAVYEIREKGIIVLSLNMPGSVPDAADLVVSDPVQCGVMAVMAVSDSASFDIRRQ
                     RGRRY"
     gene            1124..1603
                     /locus_tag="VFT03_06555"
     CDS             1124..1603
                     /locus_tag="VFT03_06555"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEU4847840.1"
                     /translation="MNERVLPERSGSRPRTTPTNPHTQLEQNPEPEVVEELARRVFAL
                     PGVEERPSAISVPGARALWLRGDIAPGPQEAFMIGREFAHIHPMPDGSLHAALPPEVA
                     REAVEKGWAEQHPVARMGYIPQNVVMIYAPRNAEEIEVVAALVVEAYRYAGGADGEG"
     gene            complement(1660..>2325)
                     /locus_tag="VFT03_06560"
     CDS             complement(1660..>2325)
                     /locus_tag="VFT03_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4847841.1"
                     /translation="TEEVASHALSMALSLLRHLPLYDRVVRDGRWHPHSTGPVHRLKN
                     LTLGVVGVGRIGGTFARRASPWFGRTLGCDPYLREDAWTREVEPASLEEVFSESFLVS
                     LHAPLTEETREFVDRGVLELMPEGGYLVNTARGDLVVMDDLLWALEEGPLAGAALDVL
                     PQEPPPRDHPLLSNPNVILTPHSAYYSLEAEKEARTKAAWNVVAWAEEGRPLYPVVEG
                     RQD"
BASE COUNT          418 a          746 c          797 g          364 t
ORIGIN      
        1 ccgacggccg cttcatgatg gcggccagcg aagtaccgcg catcccccaa atgacgtaaa
       61 tttctgcctg gcgccaatcc tacggatagg gtgcagcctc caggaaaggt cattggaccg
      121 cagatgtgca gatctccgag acgccacctg cttgcacccg gttccgtagg ccgggcacga
      181 gcgcgtaaac tctagaccag tgctccttcg atctccgact cccctcccag cgttgcggag
      241 acgcgggtct tcggggagtt gtaagttagt acggttgagg gaaagaagtc agcagggtat
      301 gtcgatagca cagaatcaca agggaaccat gaagatcccg ccgacaggtc gggctcgagc
      361 gagccgtctg gtagcctccg tcttcgagga gtcgcgcccg tgaaggagga aagtgacacc
      421 cgtctcctgg ctctgatggt ccgcacgcca gacgagccgg ggatgctcca cgcgctgacg
      481 aaggtgatct tcgatcacca ggccaacatc acctacgtcg acatcgccga gcggcgcgag
      541 gacggctcga cgacctactt cgaactcgag aacgtcgccg ccccggacga gctcatcggc
      601 gacctcgagt cgctgcccat cgtgtgggcg gtcgaacagg caccgtcgtt ccagaaggtg
      661 tacggcaagc ggatcatcgt ggtcggcggt ggggcgcagg tgggccaggt ggtggtgggg
      721 gcggtcgccg aggcagacag gcacaacatc cgcggcgaga agatctcgat cgacacgata
      781 ccgctggttg gcgaggagga gctggccgcg gcgacgcgcg ccgtagcacg cctcccgcgc
      841 gctgtggcgc tggtcctggc tggcgcgctc atgggcggag aggtcgcccg cgccgtctac
      901 gagatccggg agaaggggat catcgtgctc tcgctgaaca tgccagggag cgtacccgac
      961 gccgccgacc tggtggtgag cgacccggtc cagtgcggcg tgatggccgt gatggccgtc
     1021 tccgattcgg ccagcttcga catcaggcgc cagcggggtc ggcgctattg agtatgatcg
     1081 tggtagaggc gtacgtgacc gcgaaagtca agagggtaga aagatgaacg agcgcgtctt
     1141 gccggagagg agcgggtccc ggcccaggac cacgcctacc aacccgcaca cgcaactgga
     1201 gcagaacccg gagcccgagg tagtagagga actggcccgt agggtcttcg cccttcccgg
     1261 tgtggaggag cgcccgagcg ccatctcggt gcccggggct cgcgccctgt ggctgcgcgg
     1321 agacatcgcg cccggaccgc aagaggcgtt catgatcggg cgggagttcg cccacatcca
     1381 ccctatgccc gacggcagcc tgcacgccgc gctaccgccc gaggtcgcgc gagaagccgt
     1441 ggagaagggc tgggccgagc aacacccggt agcccgcatg ggctacatac cgcaaaacgt
     1501 ggtgatgatc tacgcgccga ggaacgcgga ggagatcgag gtcgtcgccg cgctcgtggt
     1561 ggaggcttac cgctacgccg gtggcgcgga cggagaaggg tagctcagtc agcacgctac
     1621 ggcctagcgg cctccgcttg tcagcacggc gctcccacgc tagtcctgtc tcccttcgac
     1681 gaccgggtag agcggacgcc cttcctcggc ccatgcgacc acgttccagg cagccttggt
     1741 gcgggcttcc ttctccgcct ccagcgagta gtaggcggag tgcggggtca gtatgacgtt
     1801 cgggttgctc aggaggggat gatcccgggg aggcggttcc tgcggcagga cgtcgagcgc
     1861 ggctccggcc agtggccctt cctcgagtgc ccagagcagg tcgtccatta ccaccaggtc
     1921 gccgcgcgcg gtgttgacca ggtagccgcc ctcgggcatc agctcgagca cgccacggtc
     1981 aacgaactcc ctggtctctt ccgtgagcgg cgcgtgcagc gagaccagga agctctcgga
     2041 gaagacctcc tcgaggctcg ccggctccac ctcccgggtc caggcgtctt cccggagata
     2101 cgggtcgcag ccaagggtcc tgccgaacca cgggctggct cgccgcgcga aggtaccgcc
     2161 gatacgcccg actcccacca cgccaagcgt caggttcttg agcctgtgca caggacccgt
     2221 agagtgagga tgccagcggc cgtccctcac gacgcggtcg tagagcggta ggtgcctcag
     2281 cagtgacaga gccatgctca gggcgtgcga cgcgacctcc tcggt
//
LOCUS       DASTUV010000329         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1597742, whole genome shotgun sequence.
ACCESSION   DASTUV010000329 DASTUV010000000
VERSION     DASTUV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597742"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..332)
                     /locus_tag="VFT03_06565"
     CDS             complement(129..332)
                     /locus_tag="VFT03_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEU4847842.1"
                     /translation="MTEISATNPNSFEDAVNEGVTRATSTLRNVEGAWVKDQNVLIEN
                     GNIVGYKVNLAVTFVLEDSGDIS"
     gene            complement(453..1172)
                     /locus_tag="VFT03_06570"
     CDS             complement(453..1172)
                     /locus_tag="VFT03_06570"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU4847843.1"
                     /translation="MNHERFDELKDAYALGALPEQERRELEEYLAAHPERQAEIDELG
                     NVASLLALSPLEQEPPLELRRSIMDVVEAEAQRPPARTRSWLDGVRELLSFKNLALAA
                     AALLVIGLFSWNMLLQDQVQDLQGQVASLQESQETRMVALAGTGAAQQAQVEVILIKD
                     HKAVLTAEDMPSVPENKTYQIWVIEGDVPQPSGLFKPDGGTVAAVVEKPLDEDDVIAI
                     TIEPDGGSQQPTTDPMLTAKV"
     gene            complement(1178..1768)
                     /locus_tag="VFT03_06575"
     CDS             complement(1178..1768)
                     /locus_tag="VFT03_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4847844.1"
                     /translation="MADPRRQYLLLADEDLISLVAAKGSAEAFAALYDRHGSAAYSLA
                     YRMMGEKQAAEDLMQDAFLKVWRNAGSYRSERGSVRTWILSIVHNRGIDQLRSLASRR
                     RTQDRVEASAPVSQPSEAFAQTWRNSQREQVREALGTLPAEQLKILELAYFSGYTHME
                     IAELLELPLGTVKGRMRLGLKKIRDYFDSRDVAVPR"
     gene            1876..>2171
                     /locus_tag="VFT03_06580"
     CDS             1876..>2171
                     /locus_tag="VFT03_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4847845.1"
                     /translation="MRLSVLSSGSSGNATYIESERGGLLVDAGLPCRHIENLLGRIGR
                     RLDDVGAVLVTHGHADHASGVRSLVRECGAEVFAAPGAAERLGAAWVSPGEIFEV"
BASE COUNT          349 a          700 c          678 g          444 t
ORIGIN      
        1 aaccagtcct ggaccgcggc cgtcgctctg gactggctcg cagcggacac ggcgtagaga
       61 tggccttcaa tacgaaacac cccggctacg agccggggcg ctctggttcc gcgaaagtca
      121 gctcctaatt aactaatgtc cccagaatct tcgaggacga aggtgaccgc caggttcacc
      181 ttgtagccga cgatgttgcc gttctcgatc aggacgttct ggtccttgac ccaggcgcct
      241 tccacgttgc gcagcgtgga cgtcgcccga gttacacctt cgttgactgc atcctcgaag
      301 ctattcgggt tggttgcgct tatctcggtc acgcgagcaa cactcataaa tcctccttat
      361 tggtagccaa agctctccct ctcgagacag caatataccc ggcgaagggg aaactaaacg
      421 cgaagcggag gctccgagag cctgcgcggg gctcagacct tggcggtcag catgggatcg
      481 gtcgtcggct gctgcgagcc cccatcgggc tcgatggtta tcgctatgac gtcgtcctcg
      541 tccaggggct tctcgaccac ggcggcgaca gtcccgccgt cgggtttgaa gaggccgctc
      601 ggctgaggga cgtcgccctc tatgacccag atctggtagg tcttgttctc gggcacgctg
      661 ggcatgtcct cggccgtcaa gactgctttg tgatccttga tgaggatcac ctccacctgg
      721 gcttgttgcg cggccccggt tcccgcgaga gccaccatcc gtgtctcctg cgattcttgc
      781 aagctcgcta cctggccctg cagatcctgg acctgatcct ggagcagcat gttccaggag
      841 aagagcccga tcacgagcag ggctgctgcg gccagcgcga gattcttgaa gctcaggagc
      901 tctcttaccc cgtcgagcca ggaccgggtc cttgcgggcg gtcgctgcgc ctcggcctcg
      961 accacgtcca tgatgctcct gcggagctct agcggcggct cctgttccag gggcgagagc
     1021 gcaagcaggc tggccacgtt gcccagttcg tctatctcgg cctgtcgctc gggatgcgcg
     1081 gcgaggtatt cctcgagctc gcggcgttct tgttccggca gagccccgag ggcgtaggcg
     1141 tctttgagtt cgtcgaagcg ctcgtgattc attccgttca cctcggcacc gccacgtctc
     1201 gcgaatcgaa gtaatccctt atcttcttga gccccaggcg catccggccc ttcaccgtcc
     1261 cgaggggcag ttcgagcagc tctgctatct ccatatgggt gtagccggag aaatacgcca
     1321 gctccaggat cttgagctgc tccgcaggga gggtgccaag ggcctcgcgc acctgctcgc
     1381 gctgcgagtt gcgccaggtc tgtgcgaacg cctcgctcgg ctgggatacc ggcgcggatg
     1441 cctcgaccct gtcctgtgtt ctgcggcggc tcgcgagcga gcgcaactgg tctatgccgc
     1501 ggttgtgcac gatagagagg atccaggtcc tgacgctccc tcgctccgac cggtagctcc
     1561 ccgcgtttcg ccagaccttc aggaaagcgt cctgcatcag gtcttcggcg gcctgcttct
     1621 cgcccatcat gcggtacgcc agcgagtaag cggcgctgcc gtggcggtcg tagagcgcgg
     1681 cgaaagcctc tgcgctgccc tttgccgcca cgagggagat gagatcctcg tccgccagca
     1741 gcaggtattg tctccttgga tccgccacac ccgtattcta cgtgagccgg gttgattagg
     1801 atcacagcag gcgacctccg ccgtgctaac atctctgaga gcgagtcgat ttcaccggct
     1861 gaacagagga agagtttgcg gctgagtgtc ctctcgagcg gtagctcggg caacgcgacg
     1921 tacatcgaat cggagagagg cggtctgctc gttgatgccg ggctcccatg tcgacacatc
     1981 gagaacctcc tgggccgcat aggccggcgt ctcgacgacg ttggcgccgt cctcgtgacc
     2041 cacgggcacg cggaccacgc ctccggtgtc cggtcccttg tgcgcgagtg tggcgcggag
     2101 gtcttcgccg ccccaggcgc cgcggagcgc ctgggtgcgg cgtgggtctc acctggcgag
     2161 atcttcgagg t
//
LOCUS       DASTUV010000330         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_644017, whole genome shotgun sequence.
ACCESSION   DASTUV010000330 DASTUV010000000
VERSION     DASTUV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644017"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFT03_06585"
     CDS             complement(<1..372)
                     /locus_tag="VFT03_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4847846.1"
                     /translation="MSVAPSSLGTQPIGETSRVSDGVYQVKLPVPFPLKFVASYLVED
                     CDGWTVIDPGFDYPPARGTWETCAAEVGLDLERDLARIIVTHLHPDHIGLARWLEERS
                     GAPIWMLEGEIENARRVWDPDR"
     gene            complement(397..1032)
                     /locus_tag="VFT03_06590"
     CDS             complement(397..1032)
                     /locus_tag="VFT03_06590"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4847847.1"
                     /translation="MAASDTLIRSSDREVEALAPKQARLIRSAYKVMGEKGLRHLSLQ
                     DVADEAGVSKAILPYYFESKENLILLTMRWVLARVAGRIREAIADASSAEEKVSAMID
                     AIFLSPESNRRFYLVFFDFLGYAARNDRFADVGATFHEICNGLYAEVIKLGQEEGVFR
                     GSDAREGATVVRALVDGLFTQWIQEKDWEKSHAEYREMCKRSVLTYLTADA"
     gene            complement(1089..1859)
                     /locus_tag="VFT03_06595"
     CDS             complement(1089..1859)
                     /locus_tag="VFT03_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU4847848.1"
                     /translation="MAVEYSREGSVGYITLDNPPANSYDLSFMEELGESVRSAAEDEG
                     AGAVILRSGSERFFSAGADIKAFDANSVEENMRMIRQAHEALSEMARTPKVFIAQIGA
                     TALGGGLEMALACDLRFGAEGEYVLGLPEATLGLLPGNGGTQRLPRLIGVSKALDLMV
                     TGRTISPSEAHELGILDRLFPEDALEEETRRYAEKLADGATEAIGSVKRAVYGGIERP
                     LEEGLETERTLVEPLFDSDDAREGISAFMEKRKPEFSK"
     gene            complement(1942..3387)
                     /locus_tag="VFT03_06600"
     CDS             complement(1942..3387)
                     /locus_tag="VFT03_06600"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4847849.1"
                     /translation="MAQALIGGEHVGARSGEETPVVNPATEEEFDAVPRGGPEDVDAA
                     VAAAKEAFSEWSKKDPDERAQLMRAGIAKIQENSKEIAQLLVREQGKPFTEAMGELHH
                     FLHGMNYYADLASKIEGVYANLPSALGRAYGMIIKKPIGVCAAIVPYNFPLTLMGTKV
                     GPALAAGNTIVVKPANTTPLATLRVAELMQEAELPPGVLNVVTGPGREVGEALATHPD
                     VRRVALTGSTQTGRRMMEIAAPKFKHVSAELGGSDPVIVFPDADVDAAVRGVNIGRFF
                     NAGQACLAAKRVYVFEDIYDEFMEGLAKRVSRYELGDGMEKAEKPKIRMGPLNAARHR
                     DLLADQLQDAVDRGAKVAVGGNGHASKGYFFEPTVIENAPHDSRVVREETFGPLLPIF
                     KVNDVDEAIRLANDSPYGLGSSVYTHNVKWIHRAAQEIEAGMTWVNQLHFGYDELPFG
                     GVKDSGIGREHGKEAIEYYLEQKSVVVGGLD"
     gene            complement(3389..3940)
                     /locus_tag="VFT03_06605"
     CDS             complement(3389..3940)
                     /locus_tag="VFT03_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU4847850.1"
                     /translation="MGHVLPFEDKRPLVADGAFIAPTAVLIGDVVVEEGASVWFGAIL
                     RGDVNRIVVGSGTSVQDNSVIHASEDLPTMIGSNVTVGHLSLLEGCEIEDGAVIGMGS
                     IVLNRARVGHRAMLAAGSVVRENQEIPPEVLAAGVPAEVKKDLGGSSSEWVEMAARKY
                     QALRLKYMERPEYSVSDIAKGDA"
BASE COUNT          631 a         1414 c         1187 g          757 t
ORIGIN      
        1 cctgtccggg tcccagacgc ggcgggcgtt ctcgatctcc ccctcgagca tccagatcgg
       61 cgccccgcta cgctcttcca gccagcgggc gagccctatg tgatccgggt gcaggtgcgt
      121 cacgatgatc cgcgccaggt cgcgctccag gtccagacca acctctgcgg cgcaggtttc
      181 ccaggttccg cgggctggcg gatagtcgaa gcctgggtct atgaccgtcc acccgtcgca
      241 gtcctcgacg aggtacgacg cgacgaactt aaggggaaac ggcactggca gcttgacctg
      301 atagacgccg tccgagacgc gcgacgtctc gccgattggc tgcgtgccga gagatgaagg
      361 agcgacgctc acgacaccgc gagtagcggg cggcctctaa gcatcggcgg tcaggtaggt
      421 cagcacggag cgcttgcaca tctctcggta ctccgcgtgg gacttctccc aatccttctc
      481 ctggatccac tgcgtaaaca gcccgtccac gagggctcgc actaccgtcg cgccttctcg
      541 agcgtcgctc ccgcggaata caccctcctc ttgcccgagc ttgatgacct cggcgtagag
      601 gccgttgcag atctcgtgaa acgtcgcgcc cacgtcggcg aaacggtcgt tgcgggccgc
      661 gtagcccagg aagtcgaaga agacgaggta gaacctgcgg ttcgactccg ggctcaagaa
      721 gatggcgtcg atcatcgccg agaccttctc ctccgcggag gacgcatcgg cgatcgcctc
      781 gcggatgcgt cctgcgacgc gggcgagcac ccagcgcatg gtgaggagga tcaggttctc
      841 cttggactcg aagtagtaag ggaggatggc cttgctgacc ccggcctcgt ccgccacgtc
      901 ctgcagggag agatggcgca accccttctc gcccatgacc ttgtaggcgc tgcgtatcag
      961 acgcgcctgc ttgggcgcca gcgcctcgac ttcgcggtct gacgagcgga tcagggtgtc
     1021 ggatgcggcc acgtttctct cacctcctgt gcgccggatt gtaccttaaa ggagcaaact
     1081 gttacagctt acttactaaa ctctggcttg cgcttctcca tgaacgcgga gatgccctct
     1141 ctagcatcgt cgctgtcgaa aagcggctcc accagcgtgc gctccgtctc gaggccttct
     1201 tccagcggcc tctcgatccc accgtacacc gcgcgcttta cggacccgat ggcctcggtc
     1261 gcgccgtccg ccagcttctc cgcgtacctg cgggtctcct cttccagagc gtcttccggg
     1321 aacagccggt cgaggatgcc gagctcgtgg gcctccgagg gcgagatcgt ccttcccgtc
     1381 accatcaggt cgagcgcctt gctcacccct atgaggcgtg gcaggcgctg ggtacctccg
     1441 ttgccgggga gcagaccgag ggtcgcctcg gggagcccca gcacatactc gccctccgcg
     1501 ccgaagcgca ggtcgcaggc cagagccatc tccaaccccc cgccgagggc ggtcgctccg
     1561 atctgggcga tgaacacctt aggggtcctg gccatctcgg agagcgcctc gtgggcctgg
     1621 cggatcatcc gcatgttctc ctcgacgctg ttggcgtcaa acgccttgat gtcagccccg
     1681 gcggagaaga acctctcgct gccgctgcgc aagatcacgg cgcctgcacc ttcatcctct
     1741 gccgcggagc gcacggactc gcctagctcc tccatgaagg agaggtcgta gctgttcgcc
     1801 ggcgggttgt ccagggtgat gtaccctacg ctgccttccc tcgaatactc gacggccatg
     1861 tatcctcctc tagtatctcg aacggctagc cgcaggcatg gggccgcgga cactacagag
     1921 gtccgcgacc cgccgcccct ctcagtccag tcctccgacg actacggact tctgctccag
     1981 gtagtactcg atggcctcct tgccgtgctc gcggcctata cccgagtctt tcaccccgcc
     2041 gaaggggagc tcgtcgtagc cgaagtggag ctggttcacc caggtcatgc ccgcctctat
     2101 ctcctgagct gcgcggtgga tccacttcac gttgtgcgtg tagacggagg agcccaggcc
     2161 gtaaggcgag tcgttcgcca gcctgatcgc ctcgtccaca tcgttcacct tgaagatagg
     2221 caggagtggc ccgaaggtct cctcgcgcac cacgcggctg tcgtgcgggg cgttctcgat
     2281 cacggtcggc tcgaagaagt agcccttgct ggcgtgcccg ttgccgccga cggcgacctt
     2341 cgcgccccta tctacagcgt cctggagctg gtcggcgagc aagtcacggt gcctggcggc
     2401 gttgagggga cccatcctta tcttcggctt ctcggccttc tccatcccgt caccgagctc
     2461 gtagcgggag acccgcttcg cgaggccctc cataaactcg tcgtagatat cctcgaagac
     2521 gtagacccgc ttggcagcga ggcacgcctg gcctgcgttg aagaagcggc ctatgttgac
     2581 gccgcgcacg gccgcgtcca cgtccgcgtc ggggaagacg atcacggggt cggatccgcc
     2641 cagttccgcc gagacgtgct tgaacttggg ggccgctatc tccatcatcc tccgcccggt
     2701 ctgcgtgctg ccggtgaggg ccacccgcct gacgtcaggg tgcgtggcta gcgcctcgcc
     2761 aacctccctg cccgggcccg ttacgacgtt cagcaccccc ggaggaagct ctgcctcctg
     2821 catcagctcg gcgacgcgca gcgtggcgag cggggtagtg ttggcgggtt tcaccacgat
     2881 cgtgttgcca gcggcgagtg ccggcccgac cttggttccc atcagcgtga gcgggaagtt
     2941 gtaaggcacg atcgcggcgc acacgccgat cggcttcttg atgatcatgc cgtaagcccg
     3001 gccgagcgcc gaaggcaggt tggcgtacac tccttcgatc ttgctcgcaa ggtccgcata
     3061 gtagttcatc ccgtgcagga agtggtgaag ctcccccatc gcctcggtga acggcttgcc
     3121 ctgctctcgc accaggagct gagcgatctc cttgctgttc tcctggatct tcgctatccc
     3181 ggcccgcatc agctgggcgc gctcatccgg gtccttcttc gaccactcgg agaacgcttc
     3241 cttcgccgcg gcgaccgccg cgtccacgtc ctcggggccg cccctgggaa ccgcgtcgaa
     3301 ctcctcctcc gtcgccgggt ttactactgg cgtctcctcg ccagacctgg ctcctacatg
     3361 ctctccgcct atcaacgcct gcgccatgct aggcatctcc tttcgctata tcgcttaccg
     3421 aatactccgg tctttccatg tacttgagcc tgagcgcctg gtacttgcgg gcggccatct
     3481 ccacccactc cgacgaggaa ccgccaaggt ccttcttgac ctccgccggc acaccggccg
     3541 caagtacctc gggtgggatc tcctggttct cccgcaccac gctccccgcc gccagcatcg
     3601 cccggtggcc cacgcgagcc cggttcaaga cgatgctccc catcccgatc accgccccgt
     3661 cttcgatttc acacccctcc agcaacgaga ggtggcccac cgtgacgttg gagccgatca
     3721 tggtcggcag gtcctcgctc gcgtgtatga ccgagttgtc ctgcacgcta gtcccagagc
     3781 ccacgacgat ccggttgacg tctcccctca ggatcgcccc gaaccagaca ctcgccccct
     3841 cctcgaccac cacatccccg ataaggaccg cggtcggcgc gataaaggcg ccgtcggcta
     3901 cgaggggcct cttatcctcg aacggaagta catggcccat ctgctacctc cccgtgaacg
     3961 agggcgcgcg ccgcgcgcgg gcggcccgc
//
LOCUS       DASTUV010000331         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_469127, whole genome shotgun sequence.
ACCESSION   DASTUV010000331 DASTUV010000000
VERSION     DASTUV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469127"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFT03_06610"
     CDS             complement(<1..880)
                     /locus_tag="VFT03_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU4847851.1"
                     /translation="MNIQETYGTLGLSDFEYDRILELLGRAPNYLELSLFSVMWSEHC
                     GYKNSRLLLRRFPSSGPGVLQGPGENAGVVDVGDGWALAFKVESHNHPSAVEPYEGAA
                     TGVGGIIRDVLAMGARPVALLDSLRFGPPDEGRNRYLFGEVVRGIGGYGNAVGVPTVG
                     GEVEFAGAYSGNPLVNAMCVGLIRTEDLVRSRATGEGNQVVLLGSKTGRDGIHGATFA
                     SDELTEESESRRSNVQVGDPFAEKMLIECCLRLLEEGLLVSLQDLGAAGITSSASEMA
                     AKGGVGIEIEADRVPLR"
     gene            complement(877..1539)
                     /gene="purQ"
                     /locus_tag="VFT03_06615"
     CDS             complement(877..1539)
                     /gene="purQ"
                     /locus_tag="VFT03_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU4847852.1"
                     /translation="MKIGVTIFPGSNCDRDALHAVERMGAEPVELWHADADLKGSDAV
                     IVPGGFSYGDYLRPGAIARFANVMAPLEDFARSGGPILGVCNGFQVLTEAHLLPGALL
                     RNTGMRFVCRRIRVQVETTGTPWTSSCEPGEELTLPVAHNEGNYFADPATLVRLEDED
                     RVVLRYLENPNGSINDIAGICNEGRNVVGIMPHPERVSDSLLGSEDGMRILRSVLAGA
                     RV"
     gene            complement(1536..1772)
                     /gene="purS"
                     /locus_tag="VFT03_06620"
     CDS             complement(1536..1772)
                     /gene="purS"
                     /locus_tag="VFT03_06620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU4847853.1"
                     /translation="MFETLKVRVLVRPKSGILDPQGEAVRNALPALGFGGVSTVHVGR
                     LIEMEVESADEVDAMCRRLLANPTIEEYEWEVVH"
     gene            complement(1869..>2174)
                     /locus_tag="VFT03_06625"
     CDS             complement(1869..>2174)
                     /locus_tag="VFT03_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU4847854.1"
                     /translation="EDLEFCEELGLKVNEILTPFFEERGVTLVDFKIEVGRDADGKLM
                     LADEISPDTCRFWDKETGEKLDKDRFRRDMGGVEEAYAEMLRRVKASGKDRDTESRG"
BASE COUNT          373 a          759 c          649 g          393 t
ORIGIN      
        1 cccgcaacgg gaccctgtcg gcctctatct cgataccgac gccgcccttc gcggccatct
       61 cggaggccga cgaggttatg ccggccgctc cgagatcctg gagagagacg agcagccctt
      121 cttcgaggag cctcaaacaa cactcgatca gcatcttctc cgcgaacggg tccccgacct
      181 ggacgttcga cctcctggac tcggactctt ccgtcagctc gtccgaggcg aaggtggcgc
      241 cgtgtatgcc gtcgcgcccg gtcttcgagc cgaggaggac tacctggttg ccttctccgg
      301 tagcccggga gcggacgagg tcctcggtgc ggatgagccc gacgcacatc gcgttcacga
      361 gcgggttgcc ggagtacgcc cccgcgaact cgacctcacc cccgaccgtc ggcacgccca
      421 cggcgttccc gtagcccccg ataccgcgca ccacttctcc aaacaggtag cgattccttc
      481 cctcgtccgg cggcccgaac ctcagagaat cgaggagcgc gacaggcctc gcgcccatcg
      541 cgagcacgtc ccggatgatg ccgccgacgc ccgtggccgc cccctcgtag ggctcgacgg
      601 ccgaggggtg gttgtgactc tctaccttga acgcgagcgc ccacccgtcc cccacgtcga
      661 cgacgccagc gttctcgccg gggccttgca acacgcctgg tcccgagctc gggaagcgtc
      721 tcaggagcag gcggctgttc ttgtagccgc agtgctcgct ccacattacg gagaagagcg
      781 agagctccag gtagttcggc gccctcccca aaagctccag gatgcgatcg tactcgaagt
      841 ccgagaggcc cagagtgccg taggtctcct ggatgttcaa accctcgccc ccgcgaggac
      901 cgagcgcagg atcctcatcc catcctcgga gccgagcaag gagtccgaga cccgctcggg
      961 gtgaggcatg atccccacca cgttcctgcc ttcgttgcag atccccgcga tgtcgtttat
     1021 cgagccgttg gggttctcca ggtaacgaag cacgacgcgg tcttcgtcct ccagtcgaac
     1081 gagcgtcgcg gggtccgcaa agtagttacc ctcgttatgc gcgaccggga gcgtgagctc
     1141 ctcacccggc tcgcatgagg aggtccaggg ggtccctgtc gtctccacct gtacgcggat
     1201 gcgccggcag acgaagcgca tgcccgtgtt gcggagaagc gcgcccggca ggagatgagc
     1261 ttcggtcagg acctggaagc cgttgcagac acccaggatg ggaccgcccg agcgggcgaa
     1321 gtcttcgagc ggagccatga cgttggcgaa gcgggcgatg gcgccgggac gcaggtagtc
     1381 gccgtaggag aatccgccgg gcacgataac ggcgtcagag cccttcaggt ccgcgtcggc
     1441 gtgccagagc tcgaccggct cggcacccat ccgctcgacg gcgtgcaggg cgtcgcggtc
     1501 gcagttggag ccggggaata tggttacccc tatcttcaat ggaccacctc ccactcgtac
     1561 tcctctatgg tcgggttggc gagcagacgg cggcacatcg catccacctc gtccgcggac
     1621 tcgacctcca tctctatgag cctacccacg tgtacggtgc tcacaccccc aaaacccaga
     1681 gccggaagcg cgttgcggac ggcctcgccc tggggatcga gtattccaga cttcggtcgg
     1741 acgaggacgc ggaccttcaa ggtttcaaac attaggcctc aggctacagg tttcgggttt
     1801 gaggtatctg ggtctgttcg cgtcgtctcc tagtctatac aacgattgcc gagtgcactc
     1861 tagcgccatc aaccccggct ctccgtgtcc cgatctttac ctgacgcctt gactctgcgg
     1921 agcatctcgg cgtaggcctc ctcgacgccg cccatgtccc gccggaagcg gtccttgtcc
     1981 agcttctcac ccgtctcctt gtcccagaaa cggcatgtgt ccgggctgat ctcatccgcc
     2041 agcatcagct ttccgtccgc gtcgcgcccg acctctatct tgaagtccac gagcgtaacc
     2101 cccctctcct cgaagaaagg cgtgaggatc tcgttgacct tgagtcccag ctcctcgcag
     2161 aactcaaggt cctc
//
LOCUS       DASTUV010000332         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_36628, whole genome shotgun sequence.
ACCESSION   DASTUV010000332 DASTUV010000000
VERSION     DASTUV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36628"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..819)
                     /locus_tag="VFT03_06630"
     CDS             complement(100..819)
                     /locus_tag="VFT03_06630"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEU4847855.1"
                     /translation="MRIHWVRHGKIASHRGNVPLTDEGAAQARERGRLLAEDLSPGEF
                     VHFMYAPTLRTRQTVEEIRTSMAEALGPDGDAYLLEVSEQWAIRNPDLYVAGQRVEMV
                     STAKTLAEQLCAPPVDPETLSEHHFFREFWKSPDRIGYWVRHLDPPGEDALAVARRQM
                     TFAMSLPDKQENRPVRYVLSTHSPVLRAILLCYTGEDPGEPGYLEPIDLILSENGSSE
                     LRFRGVRTLLSGTKVVKGIPR"
     gene            complement(848..1537)
                     /locus_tag="VFT03_06635"
     CDS             complement(848..1537)
                     /locus_tag="VFT03_06635"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU4847856.1"
                     /translation="MKIVDLSPAVPHGFKGPPSTDLGVQMNVRTKNGTGYWQSTQLDL
                     MSLHTGTHVESALHTVEGGEAIDEVALDRVIGEAVVLDLTPTEPTQIIDVADLEKANE
                     RLEAAGESIRSGDILLLRTDWAQRHIGTQKYFRESPGLTAEAARWIVERRPKSVGCDF
                     FEEPAARQPGWTADEFVVHGAILGAGIPLIEGLVNLRDLPPRIRFFAPFYKFSRVESA
                     PARAFALIETE"
     gene            complement(1555..1845)
                     /locus_tag="VFT03_06640"
     CDS             complement(1555..1845)
                     /locus_tag="VFT03_06640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU4847857.1"
                     /translation="MAFVVVAKWTAREGEADKVEEAIRKLTPLSREESGNLFYQAHRD
                     PENPNLFFFYEQYVDEDGYKAHGDSEHFQKYGFGTAIPLLESREREFYVTLD"
     gene            complement(1850..2686)
                     /locus_tag="VFT03_06645"
     CDS             complement(1850..2686)
                     /locus_tag="VFT03_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU4847858.1"
                     /translation="MRLVTYDAGQGPRVGILEGDAILDAGFDGDMIAFIEAGAPVGSQ
                     TPVENAQLLSPLRPRSMRDFLAFEGHLKNAFRRLGRPIPDEWYEVPAFYRGMPDTVIG
                     PEEIIPYPSYTQKLDHELELAAIIGRRGRDIPREEADSYVFGYTIWNDLSARDTQARE
                     LPVGMGPSKAKDWDGSNVLGPCIVTADEVDAGDLTMRVRVNGETWGEDNSSNMHHKFA
                     DMIAYVSQAQTIYPGEVFGSGTAEGGAGIEMDRYLSEGDVVELEIEGIGILRNSIGMK
                     GA"
     gene            complement(2683..>3150)
                     /locus_tag="VFT03_06650"
     CDS             complement(2683..>3150)
                     /locus_tag="VFT03_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU4847859.1"
                     /translation="VRFNVNGEAVEVEARAHEMLLDVLRRELGLTSVRYTCGVGICGA
                     CTVLVDGEPISTCILLALLAEGKEITTVEGLGGEHPVQRAFVEAEAFQCGYCTPGMIL
                     TAKSLLEEDPEPTEEKIRNYMGGNLCRCGCYLKIEDAVNLAASKAAAPIKGGT"
BASE COUNT          547 a         1036 c          916 g          651 t
ORIGIN      
        1 ggggcacgct tcgtccctac cttttcggct acgctgctcc ttttaggacg cggacgggct
       61 cgatgtccat cttggtgata gtcgccgccc acgtaccacc taccgtggta tgcctttgac
      121 caccttagta ccggaaagta acgttcgaac gcctcggaac cgaagctccg acgacccatt
      181 ctctgacaag atcaggtcta tgggctctag gtatcccggc tcgcctgggt cctctcccgt
      241 gtaacaaagt aagatcgccc gcagcacggg agaatgggtt gagagtacgt aacggaccgg
      301 tctgttctcc tgcttgtccg gcaagctcat ggcgaaggtc atctggcgcc gggccaccgc
      361 caacgcgtcc tcgccgggcg ggtcaagatg gcggacccaa tagcctatac gatctggcga
      421 cttccagaac tcgcggaaga aatgatgctc cgagagcgtc tcgggatcca ccggcggcgc
      481 gcagagctgt tccgcgaggg tcttggctgt gctcaccatc tctacccgct ggcccgcgac
      541 gtagaggtcc gggttacgga tggcccactg ctcgctgact tcgagcagat acgcatcgcc
      601 atcgggcccc aaggcttcgg ccatgctcgt ccggatctcc tcgacagtct gccgggttcg
      661 gagggtagga gcgtacatga agtggacgaa ctccccgggg gagaggtcct cggcgagcaa
      721 ccgccccctc tctcgagcct gggccgcccc ttcgtctgtc aaggggacgt ttcccctgtg
      781 agaagcgatc ttgccgtgcc gtacccaatg gatcctcaca gtggataaca gagcggctcc
      841 ccttagctta ctcggtttcg atcagggcga acgcgcgggc aggggcgctt tctaccctcg
      901 agaacttgta gaagggagcg aagaaccgaa tccgcggcgg tagatctctc aggttcacga
      961 gaccctcaat cagcgggatg cctgcgccca ggatcgcgcc atgtacgacg aactcgtccg
     1021 ccgtccagcc tggctgccgg gccgcgggct cttcgaagaa gtcgcagccg acggatttcg
     1081 gcctcctttc gacgatccac ctggcggcct ccgctgtcag gccgggcgac tctctgaaat
     1141 atttctgagt cccgatgtgc ctctgggccc aatcggtcct aaggagcagg atgtcgccgg
     1201 accggatcga ctcgcccgcg gcttctagcc tctcattcgc cttctcgagg tcggccacgt
     1261 ctatgatctg cgtaggctcc gtgggcgtga ggtcgaggac caccgcctcg ccgatcaccc
     1321 gatccagcgc aacctcgtct atggcctcac caccctcgac cgtgtggagg gcggactcca
     1381 cgtgcgttcc ggtgtgcagg ctcatcaggt cgagctgcgt ggattgccag taccctgttc
     1441 cgttcttcgt cctgacgttc atctgcaccc cgagatccgt cgagggtggt cccttgaagc
     1501 catgcgggac cgccggactg aggtctacga tcttcatgga gacgttcatg cctgctagtc
     1561 gagcgtcacg tagaactcgc gctcccggct ctcgaggagc ggtatcgccg tgccgaagcc
     1621 atacttctgg aagtgctcgg agtcgccgtg cgccttgtag ccgtcctcat cgacgtattg
     1681 ttcgtagaag aagaacaggt tcgggttctc cgggtcgcgg tgagcctgat aaaacaggtt
     1741 gcccgattct tcccgtgaga gcggcgtaag cttccttatt gcctcctcga ctttatccgc
     1801 ctcgccctct ctggccgtcc acttcgctac gacaacgaaa gccatatctc tatgctcctt
     1861 tcattccgat cgaattcctc agaataccga tgccctctat ctcgagctcg acgacgtccc
     1921 cctcgctcag gtagcggtcc atctcgatgc ctgcgccgcc ttcggcagta cccgagccga
     1981 aaacttcacc ggggtagatc gtctgcgcct gagaaacgta ggcgatcatg tccgcgaact
     2041 tgtggtgcat gttggacgag ttgtcctcac cccatgtctc gccgttgacg cggacgcgca
     2101 tcgtcaggtc acccgcgtcc acctcgtcgg ctgtcactat gcaggggccc aggacgttcg
     2161 agccgtccca gtccttcgcc ttcgacggac ccatccccac cggcaactcg cgagcctggg
     2221 tatcgcgggc cgagaggtcg ttccagatcg tgtagccgaa gacgtagcta tccgcctctt
     2281 cacgagggat gtccctgccc ctgcgcccga ttatggcggc gagctccagt tcgtggtcca
     2341 gcttctgtgt gtaagacggg taaggtatga tctcctccgg cccgatcacg gtgtcaggca
     2401 tcccccggta gaaggcggga acctcgtacc actcgtccgg tatcggccgc cccagacggc
     2461 ggaaggcgtt cttgaggtgt ccctcaaagg cgaggaagtc cctcatagag cgcggcctca
     2521 agggagagag cagttgagcg ttttctaccg gggtctgcga accgaccggg gcaccggcct
     2581 cgataaaggc gatcatgtcg ccgtcgaaac cggcgtccag gatggcgtcg ccctcaagaa
     2641 tccctaccct gggcccctgc ccggcatcgt aggtaaccag cctcatgtgc ctcccttgat
     2701 cggcgcagcg gctttgcttg ccgccaggtt caccgcgtct tcgatcttga ggtagcaacc
     2761 acagcgacac aggttaccgc ccatgtagtt cctgatcttc tcctccgttg gttccgggtc
     2821 ctcttcgagg aggctcttgg ctgtgaggat catgccagga gtacagtagc cacactgaaa
     2881 ggcctctgcc tcgacgaacg ctctctggac gggatgttcc ccgccgagcc cctcgacggt
     2941 tgtgatctcc ttaccctccg cgagcagggc gagcaggatg cacgtcgaga tcggctcgcc
     3001 gtccaccagc accgtgcacg ccccgcagat ccctacgccg caggtgtaac ggacgctcgt
     3061 cagaccgagc tcgcgtctga gaacgtcgag aagcatctcg tgcgccctgg cttcgacctc
     3121 gacggcctcc ccgtttacgt taaagcggac
//
LOCUS       DASTUV010000333         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_632733, whole genome shotgun sequence.
ACCESSION   DASTUV010000333 DASTUV010000000
VERSION     DASTUV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632733"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..672)
                     /locus_tag="VFT03_06655"
     CDS             complement(10..672)
                     /locus_tag="VFT03_06655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU4847860.1"
                     /translation="MPNPADFIASIPDSPRDILHFVSGLLLAHGYAVIFIGAALDNFG
                     LPASGDVVLFAGGWFANLGRVALPLVMLSGFAGALVSDNTVYWIGRIGGRPLISRVLK
                     MRFLHFLINEGTLEKVERSFEAHGGKMVFVGRFGPGLRSMTPLFAGVSRMKYYKFLPY
                     NLSAAAVWAVAYTLVGYVFGSYWSDLLAVAKSFGYGIVALVILVLGVYLIRHLLNRGE
                     KE"
     regulatory      780..914
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            969..1178
                     /locus_tag="VFT03_06660"
     CDS             969..1178
                     /locus_tag="VFT03_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847861.1"
                     /translation="MERDFRRISLGEVPLWSWLLTLLLLGMLFTLLFASGDLLVPLLG
                     HSARVANYLHEFAHDGRHLLAVPCH"
     gene            1192..1893
                     /locus_tag="VFT03_06665"
     CDS             1192..1893
                     /locus_tag="VFT03_06665"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEU4847862.1"
                     /translation="MIALYLRRGMAAGLLAGLLAGLFAYIIGEPLLDQAIGLEHSAHT
                     HDGEEVFSRSAQKVGLFFATGFTGVFVGGLFGLAFAYFRGRLASESDWTRSLSLAAAI
                     FAGAALIPFLKYPANPPTVGEPATIGARTSAYFAMVGLSLLAVLISWYASGLLRRRGV
                     STPVRHVTVGLGLATVVAALFLILPATADPGHFPAGLLWSFRLSSLGTQLVLWTGLGV
                     LFGALCERAGRRGTL"
     gene            1890..>2468
                     /locus_tag="VFT03_06670"
     CDS             1890..>2468
                     /locus_tag="VFT03_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEU4847863.1"
                     /translation="MRGTLLVAGTHSDAGKSVVVAGICRWLAREGVRVAPFKAQNMAL
                     NSFVTREGAEIGRAQAAQAAAACVQPEAAMNPVLLKPSARDTQVIVRGRPWATASARS
                     YQGMKKDLLPVVLESLEYLENRYDVVVCEGAGSPAEINLRPYDLANMGLARAAEMPVI
                     LVGDIDRGGLFASLYGTLALLSAEDQALVSGFL"
BASE COUNT          401 a          800 c          847 g          420 t
ORIGIN      
        1 tggcgcggcc tactctttct cgcctctatt gaggaggtgg cgtatcaggt agacgcccag
       61 gacgaggatg acgagagcga cgataccgta gccgaaggac ttcgcgactg ccaggaggtc
      121 gctccagtag gagccgaaga cgtagccgac gagcgtgtag gcgacagccc acacggcggc
      181 tgccgagagg ttgtagggta agaatttgta gtacttcatc ctgctcaccc cggcgaacag
      241 gggtgtcatg ctcctgaggc ccggcccgaa gcggccgacg aagaccatct tgccgccgtg
      301 ggcctcgaag gaacgctcga ccttctccag ggttccctcg ttgatcagga agtggaggaa
      361 gcgcatcttg aggacgcggc tgataagggg ccgcccgccg atccgcccga tccagtagac
      421 ggtgttgtcc gaaaccagag cgcccgcgaa gccggagagc atcaccagcg ggagcgcgac
      481 cctcccgagg ttggcgaacc atccccccgc aaagaggacc acgtcgcccg aggccggcag
      541 gccgaagttg tcgagcgccg ccccgataaa gatcacggcg tacccgtggg cgagcagcaa
      601 acccgaaacg aagtgcagga tgtcccgggg cgaatctggg atggatgcga tgaagtctgc
      661 agggttaggc atgaaccgac gttttatacc acgccgcctt acgtcttccc atgcggcgcg
      721 gttgacgcgc gcgggggcca cggctatgct gcgctgctaa gcagggggcg agggggctgc
      781 aggaagccgg tgcgagtccg gcgcggtccc gccactgtaa ccggggagcg aacccgataa
      841 aacgagccac tgccgcggct tcgagccggg cgggaaggcc ggaggcgagc gtagatccgg
      901 gagccaggag actggacctt cgcccgcaaa gatccggccg gggcgaggac cccgagaggg
      961 aggttcttgt ggaaagggat tttcgacgca tctcgcttgg agaggtgccg ctgtggagct
     1021 ggttgctgac gctcctcctc ctggggatgc tcttcaccct tctcttcgcc agcggggacc
     1081 tgctcgtacc gctgctcggg cactctgcga gggtagccaa ctacctgcac gagttcgccc
     1141 acgacgggcg tcacctgctc gcagttccct gccactaaag aggacggaca agtgatagcg
     1201 ctctacctgc gccggggcat ggcggccggg ttgctcgcag gcctccttgc ggggctcttc
     1261 gcctatatta tcggagagcc actgctcgac caggcgatag ggctcgagca ctcggcgcac
     1321 acgcacgatg gcgaggaagt cttcagccgc tctgcccaga aggtcgggct gttcttcgcc
     1381 acggggttca cgggggtgtt cgtcggaggg ttgttcgggc tggcgttcgc gtacttccgc
     1441 ggtcgcctgg cttccgagag cgactggacc cgcagcctct ctctcgcggc ggcgatcttc
     1501 gccggggccg ctttgatccc cttcctcaag tacccggcca atcctcccac ggtcggagag
     1561 cccgccacga tcggagcccg cacgtccgcc tacttcgcga tggtcgggct ctcgctgctg
     1621 gcggtcctga tctcctggta cgcctctggt ctgctccgga gacggggggt gagcaccccc
     1681 gtgcgtcacg taaccgtcgg tctggggctg gcaaccgtcg tagccgcact gttcctgata
     1741 ctccccgcta ccgcagatcc cggacatttc ccggccggcc tgctgtggag cttccggctc
     1801 tcttcactcg gcacccagct cgtcctgtgg acggggctcg gggtgctctt cggcgccctc
     1861 tgcgagcggg ccggccggag gggcacgctt tgagggggac cctgctcgtc gccgggacgc
     1921 actccgacgc ggggaagagc gtcgtcgtcg ccggcatctg ccgttggctc gcccgtgaag
     1981 gggtcagggt cgcgcccttc aaggcgcaga atatggcgct caactccttc gtgacgcgcg
     2041 agggggccga gataggccgc gcccaggccg cccaggccgc cgccgcctgc gtccagcccg
     2101 aggccgcgat gaacccggtg ctcctgaagc cctcggcgcg cgacacccag gtcatcgtgc
     2161 gcggcaggcc ctgggctacg gccagcgcga ggagctatca ggggatgaag aaggacctcc
     2221 tgcctgtcgt tctggagtct ctggaatacc tcgagaaccg ctacgatgtg gtcgtctgcg
     2281 agggcgcggg gagcccggcg gagatcaacc tgcgccccta cgacctcgcg aacatggggc
     2341 tggctcgcgc cgcagagatg cccgtgatcc tggtaggaga tattgacagg ggcgggctct
     2401 ttgcctccct ctacgggacg ctggcgctgc tctcggcgga ggatcaggcg ctcgtcagcg
     2461 gcttcctc
//
LOCUS       DASTUV010000334         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_470277, whole genome shotgun sequence.
ACCESSION   DASTUV010000334 DASTUV010000000
VERSION     DASTUV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470277"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFT03_06675"
     CDS             complement(<1..214)
                     /locus_tag="VFT03_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847864.1"
                     /translation="MFARSRASIALGYVSILALILVLFGVVVVLGFWDAANGLQDKQL
                     KQEAESRRSIVLNGDAYAGGSNDIGWS"
     gene            complement(214..900)
                     /locus_tag="VFT03_06680"
     CDS             complement(214..900)
                     /locus_tag="VFT03_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4847865.1"
                     /translation="MMRILLVEDEAYLARMIARVLGEEAYAAELAEDGRTGLSRALAE
                     DFDLVIVDWMLPDLDGLQVVRRLRAAGMSVPVLMLTARSQVEDRVEGLDAGADDYLTK
                     PFALPELLARVRALTRRPRGEPVDVVIRAGDVVLDPIHHVVRLGDVRVDLTAKEFALL
                     ATLAKRPGQVFTRSVLLDTVWGGTSDVYANVVDLYVSHLRKKLDRAGEPSLIRTVRGV
                     GYSFESRHDL"
     gene            1191..1646
                     /locus_tag="VFT03_06685"
     CDS             1191..1646
                     /locus_tag="VFT03_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847866.1"
                     /translation="MTLTETRAAGSSAVTPSRMLEAGGHLVGDDARPGVMVVFEDQYR
                     SYRETIARALKSLRPHARVSVAGLDALDAEIERVAPDLVICSHPKARAARGALVWVEV
                     PCDPTRPAAVCLEGRCSETPNLTLDELLSIVDAAEAIAIAQTITGDGPP"
     gene            complement(1755..3137)
                     /locus_tag="VFT03_06690"
     CDS             complement(1755..3137)
                     /locus_tag="VFT03_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4847867.1"
                     /translation="MRLVIVGGVAAGTKAASRARRVDPEAEIIIYQEEPEPSISECGL
                     PYFLSGMVEGREELVVRTPEKFAEKGIEVRVRHRVEKIDPEGRRLSVRALDEGEVFED
                     AYDRLIVATGARAVLPPIPGADLEGVFVLRFLTDADEVKGYIRERFPERAAVVGGGYI
                     GLEVAENLCRLGMEISLIEGEDRVALAYGPEVAEKVEAHLAENGVTVYTGTKVEEFGG
                     EGRVESVRFGNREIAADLVVVGVGVRPNVELAGEAGAEIGETGAIRVDRLMRTSLRDV
                     WAAGDCVETVNLVSGKPAWIPLGDTANQMGRVAGTNAASPAGEDTLEFPGVLGTGIFK
                     VFDLGVGKTGLSEEEAEDAGFKTICAAIETIDRARYYPGAQKVFIKLISDRSTGRLIG
                     AEATGYGADKLTDICATAIWGNLAYPDLVNLDLAYAPPFGPTLSPVIQAATVLSGQFE
                     RARRGARVSE"
     gene            complement(3467..4096)
                     /locus_tag="VFT03_06695"
     CDS             complement(3467..4096)
                     /locus_tag="VFT03_06695"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU4847868.1"
                     /translation="MAYELPPLPYPYDALEPNIDEQTMHLHHEKHHQTYVTNLNAALE
                     KHPEVDPGNIEELIANLDAVPEDIRRAVRNNGGQHSNHSTFWQIMGPSGQGGGEPTGE
                     LREAIDSSFGSFESLKEEFGAAGAPGALFGSGWVWLIANPDGSLSIETTPNGDSPLME
                     GKTPVIGLDVWEHAYYLKYQNRRPEYIGNWWNVVNWDEAERRYQAAKNA"
     gene            4223..6370
                     /locus_tag="VFT03_06700"
     CDS             4223..6370
                     /locus_tag="VFT03_06700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4847869.1"
                     /translation="MLVEISPSGVHRGSPLERRSSEELRRLLDRAVAASSNGIVITDP
                     NLPDNPIVYVNPAFEKTTGYTVEEAIGRNCRFLQGEDRDQPAIEELRASIREGQECRV
                     VLRNYRRDGTRFLNELYVSPVHDDAGNLTNFVGVQNDVTDIKRTEEVLKGSEDRLRLA
                     VESTGLGTWDFNPVTGELKWDEQCKAVFGMPPGAAVDHDVFLAGLHPDDRERTDQAVR
                     QALDPEGDGEYNIEYRTIGFTDGIERWVAARGQAYFDEAGEARRFIGTVLDITERKRA
                     EQERDLLLASEHLARVEAVRARRRLALLAAAGTTLSASLDYEATLEGIAGLVVPELAD
                     WCLVDALEEDGSVTQVAADHADPGKKLLLRQLMSHRRFGEDAPGTEAHVLRTGRSILH
                     YEVSDELLAKSATGEEHLDVLRRLEIRSLMSVPLLARGRTLGAVTLVSTDPERRYGEE
                     DLSLAEGLAYRCALAVDNSRLYSERSHIARTLQRSLLPRLAEIPGIEVGVEYLPMGQE
                     NEVGGDFYDLIHTEGERCLAVIGDVCGKGAAAAAVTVLTRYTIRAVAMREDEPSTVLR
                     GLNEAMLRQLGDEQFCTVACARLEPVSGGFELDVARGGHPAPLLVRADGYIEAVVPAG
                     RAIGVFLDPGLAVQRVRLGPGDAAVFYTDGITEARGPDGSFFGERRLRDLLRACVGLD
                     APAIAEKMRDVTLEFGEGNLRDDLALLVLRVLE"
     gene            complement(6397..>7096)
                     /locus_tag="VFT03_06705"
     CDS             complement(6397..>7096)
                     /locus_tag="VFT03_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4847870.1"
                     /translation="IYGTLFLGASALVGLVALVVALAVEESLPAVSERAHLMGASRLR
                     AAFADGPFVALLAAGVLLYYVFTQDWQALPIYAKNFLGVEDGRIGLFLGANGVMVILF
                     QLPISYAIDRTSRIWALLVGAALFAASSATLLLTESFFGILVAFALFFTLAEMILEVA
                     GAALAAELAPVRLRGTYLALFGTCFGTAYGVSPIVAGALLEARLPDFIWQIQLAAAGL
                     AAVALVVLRGFRLQ"
BASE COUNT         1234 a         2310 c         2302 g         1250 t
ORIGIN      
        1 ccgaccagcc gatatcattc gaaccgccgg cgtaggcgtc accattgagg acgatgctcc
       61 tcctgctctc cgcttcttgc ttgagctgct tgtcttgcag gccattggcc gcatcccaga
      121 acccgagcac cacgacgacg ccgaagagca cgaggatgag agcgaggatg ctcacgtacc
      181 ccagggctat gctcgcgcgg ctccgggcga acatcagagg tcatgccggg attcgaagga
      241 gtagcccacc ccgcgcacgg tccgtatgag ggacggctcg cctgcgcggt cgagcttctt
      301 gcgcaggtgc gagacgtaga ggtcgaccac gttcgcgtag acgtcgctcg tcccgcccca
      361 gacggtatcg aggaggaccg agcgggtgaa gacctgcccc gggcgtttcg ccagggtagc
      421 aagcagcgcg aactccttcg cggtgaggtc tacgcgaacg tcgcccagcc ggaccacgtg
      481 gtggatagga tcgagcacta cgtcgccagc cctgatgacc acgtcgacgg gctccccgcg
      541 cgggcgccgt gtgagcgccc gcacgcgggc gaggagctcg ggcagggcga acggcttcgt
      601 gaggtagtca tcggctccgg cgtcgaggcc ctcgaccctg tcctccacct ggctgcgcgc
      661 cgtgagcatc aacacgggca cgctcattcc cgccgcacgc agcctgcgca ctacctgcaa
      721 accgtccagg tcgggaagca tccagtccac gatcaccagg tcaaagtcct cggccagcgc
      781 gcgcgagagg ccggtacggc cgtcctcggc caattcggcc gcgtatgcct cctcgccgag
      841 tacccgggcg atcatccggg cgaggtacgc ttcgtcctcc acaagcagga tccgcatcat
      901 tgctccacca cgccttagtt tacagtaacg gcgggacgcg ctttcctgtc gaaatcttca
      961 tgattccgac aagttaagga cagactcgag gtccagactt acgggcaaat cgtgcgcaga
     1021 ccgatggcaa agggatacag gttgtccggc aagggaccga taccctcacc tgcgccaacg
     1081 gatcgcggcg ggtgatgcgc ccgcgggctc gtaaaggtca ggaggaccag atggtagaga
     1141 gcgcgacacc caccctggtt cggtcgagga accgaggacg tggcgtaccc atgaccctca
     1201 cggagactcg tgccgccgga tcgtcggccg tcactccttc gcgcatgctc gaagcaggag
     1261 gacatctcgt gggagacgac gcgaggcccg gggtcatggt ggttttcgag gaccagtacc
     1321 gatcttacag agagaccata gcgcgcgctc tcaagagtct ccgtccgcac gccagggtta
     1381 gcgtcgccgg gctggacgcc ctcgatgcgg agatcgagag ggtagctccg gacctggtga
     1441 tctgcagcca cccgaaagcc agagccgctc gcggcgcact ggtctgggtc gaggttcctt
     1501 gcgatccgac ccgtcccgca gctgtctgcc tcgaaggacg gtgttccgag acccctaatc
     1561 tcaccctgga cgagttgctc tcgatcgtcg acgcggcgga ggctatcgcc atagcgcaaa
     1621 ccatcaccgg cgacggcccg ccgtgaagac tccagggcct accagtcaac atcgctggta
     1681 gcccgcatgt tcccaagccc ttgatgctgg gcttgccgcg gtgagtcccg gtgccagaga
     1741 gagaaagttc gcctttactc agataccctg gcgcccctgc gagcgcgctc gaactggccc
     1801 gagagcacgg ttgcggcctg gatgacaggg ctgagggtgg ggccgaaggg tggggcgtag
     1861 gcgaggtcga ggttgaccag gtcggggtag gcgaggttgc cccagatggc cgtggcgcag
     1921 atgtccgtga gcttgtccgc gccatagccg gtggcttcgg ccccgatcag ccgcccggtt
     1981 gacctgtccg agatcagctt gatgaacacc ttctgcgcgc cggggtagta gcgcgcacgg
     2041 tcgatggtct ctattgcggc gcaaatggtc ttgaagcctg cgtcctcagc ctcctcttcc
     2101 gagaggccgg tcttcccaac cccgagatcg aagaccttga agatgccggt gccaagcaca
     2161 ccggggaact ccagcgtgtc ctcgccagcg gggcttgcgg cgttggtgcc agcgacgcgg
     2221 cccatctggt tggccgtgtc gcccagggga atccaggccg gcttcccgct caccaggttt
     2281 accgtctcca cgcagtcccc ggctgcccac acgtctcgca aactggtcct catgagccgg
     2341 tccacccgga tcgcgcccgt ctccccgatc tcagcccccg cctcccctgc cagctcgacg
     2401 ttcggcctca cgcctacccc cacgacgacg aggtcagccg cgatctcccg gttcccgaac
     2461 ctcacgctct cgacgcgccc ctcgcccccg aactcctcga ccttcgtccc ggtgtagacg
     2521 gtcacgccgt tctccgcgag gtgagcctcg accttctccg cgacttcggg accgtaggcc
     2581 agcgcgaccc tgtcttcgcc ctcgatcagg gatatctcca tcccgagccg gcacaggttc
     2641 tccgcgacct cgagcccgat gtaccctccg cctacgacgg ccgccctctc cgggaagcgt
     2701 tcccgtatgt accccttcac ctcgtcagcg tccgtgagaa agcggagcac gaagacaccc
     2761 tcgaggtcgg cgcccgggat cggtgggagc acggcccgcg cgccggttgc gacgatgagc
     2821 cggtcgtaag cgtcctcgaa gacctcacct tcatcgagag cgcgcacgga tagcctcctg
     2881 ccctcggggt ctatcttctc gacccggtgg cgtaccctta cctcgatgcc cttctctgcg
     2941 aacttctcgg gcgtccgaac cacgagttct tcccgaccct ccaccatgcc agacaggaag
     3001 tagggcaggc cgcactcgct gatggagggc tcgggctctt cctggtagat aatgatctcc
     3061 gcctccgggt ccaccctgcg agccctcgac gccgccttgg tccccgcagc tacccctcca
     3121 actatcacca agcgcacagc cggacacgac ctttctccag gtttatcact gcgccttagt
     3181 gtaccggcga atcccaagcc gaagggaatt tctatcacca tggccgcgaa agccttttgg
     3241 ataggacgat cgccacgggc gagtcctcgt gcctgcgacc ataaacctgt acaggaaatc
     3301 cgtcctccac ccgatgtgcc cggtcccccc tccgcgttct gatggaggtg tactcgagag
     3361 cgcgaccgaa ggagattaga ggatgtacct ccgcacgata aagttcgccc cccggaagag
     3421 cattccaggg ggcgagcttt tctctacctg ctgtctgtgg agggctctag gcgttcttgg
     3481 cagcctggta tcgcctttct gcctcgtccc agttgaccac gttccaccag ttccctatgt
     3541 actcaggacg gcggttctgg tacttgaggt agtaggcgtg ctcccacacg tccaacccta
     3601 ttacaggagt cttgccctcc atcagcgggc tgtcaccgtt gggggtggtc tctatggaga
     3661 gggacccatc ggggttggct atcagccaca cccacccact gccgaacagt gcccctggtg
     3721 caccagcagc cccgaactcc tctttgaggg actcaaagga tccaaacgaa gagtctattg
     3781 cctcccttag ctcccctgtt ggctctcctc caccctgtcc gctggggccc attatttgcc
     3841 agaacgtgga gtggttggag tgctggcctc cgttgttcct tacagccctt cttatgtctt
     3901 caggcacagc gtcgaggttg gctattagct cttctatgtt gcctgggtca acttctgggt
     3961 gcttctcgag ggctgcgttt aggttggtga cgtaggtttg gtggtgtttt tcgtggtgga
     4021 ggtgcatggt ctgttcgtct atgttgggtt cgagagcgtc gtaggggtat ggtagtggcg
     4081 gcagttcgta agccacttct tctctcctct ccttgctttg cgctacccgc ttaccttacc
     4141 cggttagtct ttatctttaa cgttcggctg cccctcgtcg tactgacgtg gatgtgagcg
     4201 gggatcgtgg gcgctgtaga atatgctggt ggagatttcg ccctcggggg tccatcgtgg
     4261 cagtcctctc gagcgccgga gctcggaaga gttgcggcgt ttgctggacc gggcggtcgc
     4321 ggccagctcc aacggtatcg taataaccga tcccaatctg ccggacaacc ctatcgtcta
     4381 cgtcaacccc gccttcgaga agacgacagg gtacacggtg gaggaagcga tcgggcgcaa
     4441 ctgccgcttc ttgcagggtg aagaccgcga ccagccagcg atcgaagagc tgcgggctag
     4501 catccgtgaa gggcaggagt gccgggtcgt cttgaggaac taccgcaggg acgggacccg
     4561 cttcttgaac gagctctacg tctctccggt ccacgacgat gcgggcaacc tcaccaactt
     4621 cgtcggcgtg cagaacgacg tgaccgatat caagcggacc gaggaggtgc tcaaggggag
     4681 cgaagaccgg ttgcgtctcg cggtcgagtc gacggggctg ggaacatggg acttcaaccc
     4741 cgtcaccggg gagctcaagt gggacgagca gtgcaaggcg gtgttcggga tgccgccagg
     4801 ggccgctgtg gatcacgacg tgttcctggc cgggctgcac ccggacgaca gggagcggac
     4861 ggatcaggcg gtgcggcagg cgctagaccc tgagggcgac ggcgagtaca acatcgagta
     4921 caggaccata gggtttacgg acggaatcga gcgctgggtt gcggcgaggg ggcaggccta
     4981 tttcgacgag gcgggcgagg cccgccggtt tatcgggacg gtccttgaca taaccgagcg
     5041 caagcgtgcc gagcaggagc gggatctgct gctcgccagt gagcacctcg cgcgtgtgga
     5101 ggccgtgagg gcgaggcgcc ggctcgctct gctcgccgcg gctggaacga cgctctcggc
     5161 ctcgctcgac tacgaggcga cgctggaggg gatagcaggc ctcgtggtcc cggagctcgc
     5221 ggactggtgt ctggtggacg cactcgagga agatggctcg gtgacacagg tcgccgccga
     5281 ccacgctgac ccgggaaaaa aactccttct gcgccagcta atgagccacc ggcgcttcgg
     5341 agaggatgcc cccggaaccg aggcgcacgt gctgcgtacg ggccggtcca tcctccacta
     5401 cgaggtctcg gatgagctac tggccaagag cgcgacggga gaggagcacc tcgatgtgct
     5461 ccggaggctg gagatccgct ccttgatgag cgtgccgcta ctggcgcggg ggaggacgct
     5521 cggcgccgtg acgctcgtct ccaccgaccc cgagaggcgc tacggcgagg aggacctctc
     5581 gctggcggag ggcctcgcct accgctgtgc cctggctgtg gacaactccc gcctctacag
     5641 cgagcggagc cacatcgccc gtaccttgca gcggagcctg ctgccgcgcc tcgccgagat
     5701 tccggggatc gaggtcgggg tagagtacct gcccatgggg caggagaacg aggtaggggg
     5761 ggatttctac gacctgatcc acaccgaggg ggagcgctgt ctggccgtga tcggggatgt
     5821 ctgcggcaag ggtgccgccg cggcggcggt gactgtcctg acccgctaca ccataagggc
     5881 ggtcgcaatg cgggaggacg agccctcgac cgtactccgc ggcctgaacg aagccatgct
     5941 gcggcagctt ggcgacgaac agttctgtac ggtggcctgc gcccgcctgg aaccggtgtc
     6001 gggcggtttc gagctggacg tggcgcgcgg cggccacccg gcgccgctcc tggtgcgcgc
     6061 cgacggctac atcgaggcgg tagtacccgc ggggagggcc atcggcgtgt tcctcgaccc
     6121 cgggctcgcg gtccagaggg tccgcctggg gccaggggac gccgccgtct tctacaccga
     6181 cggcataacg gaggcgcgtg ggccggacgg gtccttcttt ggcgagcggc ggctgcggga
     6241 tctcctccgc gcgtgcgtcg gtctcgacgc tcccgccatc gccgagaaga tgagagatgt
     6301 taccctcgag ttcggagagg gtaacctgcg cgacgacctg gccctgctag tcctgcgcgt
     6361 cctcgaatag gtttcaggat ctgggcctcg ggtttattac tgaagcctga aacctctcaa
     6421 aaccacgagc gcgacggcgg ccagcccggc cgcggcgagc tgtatctgcc agatgaagtc
     6481 gggcagccgg gcttcgagga gcgctccggc tacgatgggg ctcacgccgt aggccgttcc
     6541 gaagcaggtg ccgaagagcg cgaggtaggt cccgcgcagc ctcacgggcg cgagctcggc
     6601 cgccagggct gcccccgcga cctccaggat catctccgcc agcgtgaaga acagggcgaa
     6661 ggccacgagg atgccgaaga acgactcggt gaggagcagc gtggccgagg aggccgcgaa
     6721 cagggctgcg ccgacgagca gcgcccagat cctggaggtg cggtctatgg cgtaggagat
     6781 cgggagctgg aacaggatga ccattacccc gttcgctccc aggaacaagc cgatcctacc
     6841 gtcctctaca ccgaggaagt tcttggcgta gatcgggagc gcctgccagt cctgggtgaa
     6901 gacgtagtag agcaggaccc ccgctgcgag gagcgccacg aacgggccgt ccgcaaaagc
     6961 agcccgcagc ctcgacgcac ccatcaggtg cgcccgctcc gaaactgcgg ggagcgactc
     7021 ctcgaccgcc agcgcgacga ccagcgccac cagccccaca agggcggagg cgcccagaaa
     7081 gagcgtcccg tagatc
//
LOCUS       DASTUV010000335         5538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_235512, whole genome shotgun sequence.
ACCESSION   DASTUV010000335 DASTUV010000000
VERSION     DASTUV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235512"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            760..1572
                     /locus_tag="VFT03_06710"
     CDS             760..1572
                     /locus_tag="VFT03_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847871.1"
                     /translation="MSDQLIIAGFHRSGTSATAQLLHRAGLFLGYELLEALPSNPYGH
                     FEDREIVDLHHQIFMDNDMTWLVGEPFLPLVRGPRWQRMRQIIERRNSEQALWGFKDP
                     RASLFLALWKYLLPDARVLLVYRHFSESTYSLSQRHSSNLFTGEGNAYIHERFWEEPD
                     LALRMWLVYNNALLAFARAYPEDTLAVSLDMIRDGFPVVWAIERRWGLGLRDVPVGET
                     YDPRITGRRTRRQPVSDRRLIPKVEAAWRELEDLGKETEKMLVQKEAAVVGE"
     gene            1559..1987
                     /locus_tag="VFT03_06715"
     CDS             1559..1987
                     /locus_tag="VFT03_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847872.1"
                     /translation="MSENDFYTPTDLDMLRMENELLAFEVRFLRARLGWPGRGSGSSP
                     SLTRLAQLEEAESDLVLLVRRMSSSPLGPFLRLRGEFRTLEERYLSPEAQEASSRDRL
                     AYLELAEQDLVLLLQRMGRGPLGRVFRLKREFRTLEERYL"
     gene            2000..3157
                     /locus_tag="VFT03_06720"
     CDS             2000..3157
                     /locus_tag="VFT03_06720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4847873.1"
                     /translation="MAASNVAVGHPVFYAGRFVHRVTGLPEGCELLGDWDVLPAQLAA
                     AMERADTLVVLDPLSFPFEALTGTQQDVPLIVVLPWELDVDILVAVFGEPVFARLGFF
                     DRVATPDSSVWELLCRRYGWAEGQRVRIEGSSPEEAAREIGIVLEAEAEVLEYFGDGS
                     YEANRYWSERGAALAGSMPHRAICSVLLGSSFNKAMHRVQRTALEPQFAAARGARAED
                     IPFDVLEVGAGIGRWAASFDPAGTRFCGVDISEGMVAAARANFPQRSFEQLGPDLGFP
                     YEDESFDLVFSVTVMHHNPTPAKRTLLSEMWRVAKPGGRLLFLENFVTGGWSEKSTVY
                     PMSVLGFVELLLEATAGRVVLEHVESLRYPHDDWVRDGIIAVSKLGVPKKW"
     gene            3151..4311
                     /locus_tag="VFT03_06725"
     CDS             3151..4311
                     /locus_tag="VFT03_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847874.1"
                     /translation="MVDEKVFILSNDVVPSLGMPVAAPGLRAFGLAEGLRANGVKVKT
                     LVTRGFTDRQWLRFGRSVPHPTAPHAEVVSARRLARYLETNAPAVAVLINSNQVDHLR
                     PIRGVRYVLDFFAPKMLETLYQHGEGYPRDELAALRRRKIQAIELADAFIVNGKKKLP
                     YFLAWMLQADRDIRSLPVEVVNMCAPVSFSGEAGPAGSGVRFAVAGYLQSWSTLGSWV
                     KVVERQLERPGMRLDLLLPWHWGGAAERSHESRADLDRLERNGSVTTHGPMTFSEFQA
                     FLSTIDVSIDLFQHNLEREYAMVTRSVISLACGVPVIHPPFTEVSPMIAEYDAGWLID
                     PPDTKALEDVIGGILDDPDLVRRKKENARALASEVLDPAVAVKPLLRIMEGW"
     gene            4311..5357
                     /locus_tag="VFT03_06730"
     CDS             4311..5357
                     /locus_tag="VFT03_06730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4847875.1"
                     /translation="MGEWKRMYFMTPTFRPVGGVVKVFDYVNHARSLGYEPVIACPEP
                     YKPGLPLFEIPRFSGISPDNGVKFTGLEKVTVGPHDLAFLSWPTHYEIVEPRLSRWTR
                     HEQVIFIVQNVRWANPSFTGGYALRLLSRPMARIMTNDVVLEAVKPYLNPSSMSEVIL
                     LGNESSFFAKERRGGLGNPVKVGYTTWKSGVGDEVARILAGDRHFEFRAIREPVGWKE
                     LRELYHWSDVFLATPLVEEGFYMPGLEAMAAGAVVISSDAGGNRAYCRFGENCVGVGF
                     EDAGGYVEALQSLRKGSAQEVERLRGAGYEAVKLHTLEHERERFGEFMERLIHRLDGV
                     VPGSRPLDASPGSR"
BASE COUNT          971 a         1722 c         1809 g         1036 t
ORIGIN      
        1 atgaggagcc ggtccccaaa gcccacgtct tcgcagctaa tgagagctac cgattttcct
       61 tcggcatacc gctgggcaaa tctatacctg atcaggttat cgtaagctct ctctgctttc
      121 gtaggttcca atcttgcgag ctgctggcct atatccatcg cctacctatc tcctcccgca
      181 agcaacggcg atgactcatc acttgtttcc cacgaaccgg cttcaactga tcgctccgag
      241 ctatcggtcc tctcgagttg agctccccgc tgattttggc cgctatgcca ggaaggcctg
      301 ttaacttcct tttgacccca aaagatcgtc caatgatcac aggaccggcg ctccatctca
      361 tcgatattac agtagctcca tgttcaacgt actatagatc ataagatcac ctgcagcgag
      421 ttgctcttcg aggtggcgag ttcgttaaac tgtgtcgctc gtgcgctgtg tcttgaggat
      481 acgctgtgag gacaaactga taagtcgttg attcggggtc cgcctgaagc agatccacga
      541 cctctttcgg gacttgactg ttgtttatct ctatctccgt gtcgaatagt ccaaggcagg
      601 tactaaacga cgtcagggga gcaaggtggg aggagattga tcgcggaatg gtcggcgcgc
      661 tcccggatag atagagcggc gctctggtct acaatatgca gtcgccggct tgaatttgta
      721 tagtgcctgg cgagaccatg tggaagagga cgtaggattt tgagcgacca gttgatcatc
      781 gcgggtttcc accgcagcgg cacgtctgcg accgcccagt tgttgcatcg ggcgggactg
      841 ttcctcggct acgagttgct cgaggcgctc ccctcgaacc cttacgggca cttcgaggac
      901 agggaaatag tggacctcca ccaccagatc ttcatggaca atgacatgac ctggctggtc
      961 ggggagccat tcctaccact ggtgaggggg cctcgctggc agcgcatgcg gcagataatc
     1021 gagcgccgga actcggagca agctttgtgg ggcttcaagg acccgcgagc gagcttgttc
     1081 ctcgccctct ggaagtacct ccttccggac gccagggtcc tgctcgtcta ccgacacttc
     1141 tcggaatcga cgtactcgct gagccagcgt cactcctcca acctctttac gggcgagggg
     1201 aacgcctaca tacacgagcg gttctgggag gagccggatc tagcgctgcg gatgtggctg
     1261 gtgtacaaca acgcgctgct cgccttcgcc cgcgcttacc cggaggacac cctggccgtg
     1321 tcgctggaca tgatccggga cggcttcccc gtcgtctggg ccatagagcg gcgctggggg
     1381 ctgggcctga gagatgtgcc cgtgggagag acctacgatc ctcgcataac cggccgtcgg
     1441 acgaggcggc aaccggtctc ggaccggcgc ctgatcccga aggtggaggc ggcctggcgg
     1501 gagctagagg acctgggcaa agagactgag aagatgctgg tgcagaagga ggcagcggtt
     1561 gtcggagaat gacttctaca caccgaccga cctggacatg cttaggatgg agaacgagct
     1621 gctggccttc gaggtgcgct ttctcagggc ccggctgggc tggccgggga gaggctccgg
     1681 ctcttccccg tccctgacac gcctggccca gctcgaagaa gccgagagcg atctggtgct
     1741 gctggtcagg cgtatgtcgt ccagcccgct cggcccgttc ttgcggctca ggggggagtt
     1801 caggaccctg gaggagcgct acctctcgcc ggaggcgcag gaggcgtcgt cccgggaccg
     1861 gctggcgtac ctggagctgg cggagcagga cctcgtcttg ctgttgcagc ggatggggag
     1921 ggggccgcta ggccgggtgt tcaggctcaa gagggagttc cgaaccctcg aagagcggta
     1981 cctgtaagca ccgatgggcg tggctgcttc aaacgtcgcc gtcggccatc cggtcttcta
     2041 cgccggtcgg ttcgtgcacc gggtaaccgg cctgcccgaa gggtgcgagc tgctcggcga
     2101 ctgggacgtc ctccccgccc aactggccgc cgccatggag cgtgccgaca ccctggtggt
     2161 gcttgacccg ctctcgttcc ctttcgaggc cctgaccggg acccagcagg acgtcccgct
     2221 catcgtggtc cttccttggg agctcgacgt ggacatcctc gtcgcagtct tcggcgagcc
     2281 cgtcttcgcg cgcctgggat tctttgaccg cgtggcgact ccggattcga gcgtgtggga
     2341 gctgctgtgc cggaggtacg gctgggccga gggccagcga gtccggatcg aaggttccag
     2401 ccccgaggaa gcggcacggg agatagggat agtgctagag gcggaagcgg aggtgctgga
     2461 gtacttcggc gacggcagct acgaggcgaa ccggtactgg agcgagcgcg gcgccgcgct
     2521 cgcggggtcg atgccgcacc gcgcgatctg cagcgtactg cttggctcca gcttcaacaa
     2581 ggccatgcac cgggtgcaga ggaccgcgct cgaaccgcag ttcgcagccg cgcgtggcgc
     2641 ccgcgccgaa gacatcccct tcgatgtcct cgaggtcggc gccgggatcg gccgctgggc
     2701 ggcgagcttc gaccccgccg ggacgcgctt ctgcggggtg gacatcagcg agggcatggt
     2761 cgccgccgcc cgcgccaact tcccgcagag gagcttcgag caactcgggc ccgacctcgg
     2821 cttcccctac gaggacgaga gcttcgacct ggtcttctcc gtgaccgtca tgcaccataa
     2881 ccccacgccg gccaagagga ctctgctatc tgaaatgtgg agggtagcga agccgggtgg
     2941 acgcctcctc ttcctcgaga acttcgtcac cgggggatgg tcggagaagt caacggtgta
     3001 tcccatgtcg gtgctcggct tcgtcgagct gctcctcgag gcgacggccg gacgtgtggt
     3061 cctcgagcac gtggagtcgc tccggtaccc gcacgacgac tgggtgaggg atgggatcat
     3121 cgccgtctcc aagctcgggg tgccgaagaa gtggtagacg agaaggtctt tatactctcc
     3181 aacgacgtcg tcccgagcct cgggatgccc gtcgccgcgc cgggcttgcg ggcgttcggg
     3241 ctcgcagagg gcctgcgcgc caacggtgta aaggtgaaga ccctcgtcac ccgcggattc
     3301 accgaccgcc agtggctgcg gttcggccgg tccgtcccgc acccgacggc gccccacgcc
     3361 gaggtcgtca gcgcgcggcg gcttgcccgc tacctcgaga ccaacgcgcc cgccgtcgcg
     3421 gtgctcataa acagcaacca ggtggaccac ctgaggccca tacggggcgt ccggtacgtg
     3481 ctggacttct tcgcgccgaa gatgctggag acgctctacc agcacggcga ggggtacccc
     3541 agggatgagc tcgcggcgct caggcgccgc aagatccagg ccatagagct cgccgacgcc
     3601 tttatcgtta acgggaagaa gaagctccct tacttcctgg cctggatgct ccaggccgac
     3661 cgcgacatac gttctttgcc ggtcgaggta gtcaacatgt gcgcccctgt ctccttctcg
     3721 ggcgaggccg gacctgcggg gagcggcgtt aggttcgccg ttgccggcta cctgcagtcg
     3781 tggagcacac tcgggagctg ggtgaaggtg gtcgagcgcc agctggagcg gcccggcatg
     3841 agactcgacc tcctcctgcc gtggcactgg ggcggggcgg cggagcgctc ccacgagtcc
     3901 agggccgacc tcgaccggct cgagcggaac ggctccgtga ccacccacgg ccccatgacc
     3961 ttctccgagt tccaggcttt tctgtcgacg atcgacgtct ccatagacct cttccagcac
     4021 aacctcgagc gggagtacgc gatggtcacc cgatccgtga tctctctggc gtgcggggta
     4081 cccgttatac acccgccgtt cacggaggtg tctcctatga tcgccgagta tgacgccggc
     4141 tggcttatag accctcccga cacgaaggca ctcgaagacg tgatcggagg catcctcgac
     4201 gacccggacc tcgtgcggcg gaagaaagag aacgccaggg cgctcgcctc ggaggtcctc
     4261 gaccccgcgg tggcggtgaa acccctctta cggatcatgg agggctggta gtgggtgagt
     4321 ggaagcgcat gtacttcatg acgccgacgt tccgtcccgt tggcggggtc gtcaaggtct
     4381 tcgactatgt caaccacgcc cgctctttgg gctacgagcc cgtaatcgcc tgccccgagc
     4441 cgtacaagcc agggctgccg ctcttcgaga tcccgcgctt ctccggcatc tcaccggata
     4501 acggcgtcaa gttcaccggc ctggagaagg tcaccgtcgg gccacacgac ctcgccttcc
     4561 tctcctggcc gacgcactac gagatcgtgg agccgcgcct cagcaggtgg actcggcacg
     4621 agcaggtcat ctttatcgta cagaacgttc gctgggcaaa cccttcattc acgggcggct
     4681 acgccctgcg cctcctctcg cgcccgatgg cccgcatcat gacgaacgac gtggtcctcg
     4741 aggcggtcaa gccgtacctg aacccctcct cgatgagcga ggtgatcctg ctcggcaacg
     4801 agtcgagctt cttcgcgaag gagcgaaggg gcggtctcgg caaccctgtg aaggtcggct
     4861 ataccacctg gaagagcggt gtgggagatg aggtagcgcg gatactcgcg ggcgaccggc
     4921 atttcgagtt cagggcgata agggagcccg tcgggtggaa ggaactgcgg gagctctatc
     4981 actggtcgga cgtctttctc gcgacgcctc tcgtcgagga aggcttctac atgccggggc
     5041 tcgaggcgat ggccgccgga gcggtcgtga tctcctcgga cgcgggcggc aaccgggcct
     5101 actgccgctt cggcgagaat tgcgtcgggg tcgggttcga ggacgccggg ggctatgtcg
     5161 aggcgttgca gagcctgcgt aaaggctctg cgcaggaggt agagcggttg cgcggggcag
     5221 gctacgaggc cgtcaagctg cacaccctgg agcacgagcg ggagcgcttc ggcgaattta
     5281 tggagcggtt aatacatcgc ctagatggcg ttgtgccggg gtcccggccc ctcgatgcgt
     5341 cgcctggcag tcgctaatag agacttgaca agcccggcgc tcgattcgat gatattccag
     5401 ttgcgccggg tcaaagagca ggtctcaaac accccaggag gacgatagat gctcccggac
     5461 ttcatcggaa taggggctca gaaagcgggc acgacgtggc tgcaccgcaa cctccaggcg
     5521 catccccagc tctacatg
//
LOCUS       DASTUV010000336         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_680375, whole genome shotgun sequence.
ACCESSION   DASTUV010000336 DASTUV010000000
VERSION     DASTUV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680375"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..568)
                     /locus_tag="VFT03_06735"
     CDS             complement(47..568)
                     /locus_tag="VFT03_06735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4847876.1"
                     /translation="MTISGDLTLRDGVSVPVREVRAEDAGALRRLVNRSSERSIELRF
                     FGPMKELSEERARSFAEVDGVDRFALVALDPEDEGEIVAVVRYEREAGTDGAEYAALV
                     EDRFQGKGLGIGLTRQLIEAARERGIGSLHALVMRENRVMLGLLRSLELPERQRWENG
                     LEHIEIDLRLEAA"
     gene            complement(672..1103)
                     /locus_tag="VFT03_06740"
     CDS             complement(672..1103)
                     /locus_tag="VFT03_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847877.1"
                     /translation="MAETRDEADRAELRITSEEARHVADLLRYVISLNTTVGLVSPTQ
                     RRVATSTYKAVELAALILEGKTFDEAVEESDKKWTGSLEEDHRRALELLQKNRETLRE
                     AMSSRLTPELLAEYLEVQETQYLEMIKPKTTKERGPEAPGG"
     gene            complement(1107..>2040)
                     /locus_tag="VFT03_06745"
     CDS             complement(1107..>2040)
                     /locus_tag="VFT03_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4847878.1"
                     /translation="HVQSGLAMTAIHELGSEEQKERWLPPMARCEKVGCFGLTEPEAG
                     SDPGSLATTAVARDGGYVLDGEKKWIGNASFADVAVIWARTEDGKIAGFLVEGDNPGY
                     RAEVLSRKGSQRAAWQTHIELKDCRIPEENRLPGAEGLGSTLSVLTHSRYGVGWDGLG
                     QATDCYETALAYAGERVQFGQPIAAFQLVQQKLVMMLNEISLSQLLSVHVGRLKDAGQ
                     LDAPTVSMFKMNNVAKARQIAATARDVLGGNGVLLDYRVMEHMADIEGVYSYEGTNDV
                     NTLIVGQAITGHRAFDPRPPKSGGKAQRAEEKSQ"
BASE COUNT          307 a          679 c          638 g          416 t
ORIGIN      
        1 agccggagcg tgctgacaag cctcccgaag ggaggcgtgc tgaaaatcat gcagcttcca
       61 gccgaaggtc tatctcgatg tgctcgaggc cgttctccca gcgttgccgc tcgggaagct
      121 ccaggctgcg caacaggcca agcatcacgc ggttctcgcg catgaccagc gcatgcaggc
      181 tcccaatgcc cctctcgcgg gcagcctcta tcagctgccg cgtcagcccg atccccagcc
      241 ccttcccctg gaacctgtcc tctaccagcg ccgcgtactc ggccccgtct gtgccagcct
      301 cgcgctcgta gcgcaccacc gctacgatct caccctcgtc ctcggggtcc agcgctacca
      361 gcgcaaagcg gtcgacgcca tcgacctcgg cgaagctgcg ggccctctcc tcggagagct
      421 ccttcatggg gccgaaaaat cgcagctcta tcgaacgctc gctcgaccgg ttcacgagcc
      481 gccgtaaagc cccggcatcc tcagcccgga cctcgcgtac cggaacggaa acgccatccc
      541 ttaacgtcag atccccgcta atcgtcacgc agacctcgat acttcttctc tatgtgcgtt
      601 ctgcacataa aataattgta ccgcggtctg ggcgaggaga aacaccggtg ctgggctttc
      661 ctgttaagga attaaccgcc tggcgcttcg ggcccgcgct ccttcgtggt tttcggcttg
      721 atcatctcca ggtactgggt ttcctggact tcgaggtatt ctgcgagcag ttcgggggta
      781 aggcggcttg acatagcttc tctgagggtt tcgcggttct tctgcaggag ctcgagggct
      841 ctacggtggt cctcttcgag ggagccggtc cacttcttgt cggattcttc gacggcctcg
      901 tcgaaagtct tgccctccag gatcagggcc gcgagctcga cggctttgta tgtggaagtc
      961 gccacgcggc gctgggtggg ggagacgagc ccgacggtgg tgttgaggct gatcacgtat
     1021 cggaggaggt ctgccacgtg ccgggcttcc tcgctcgtga tcctcagctc cgcccggtcg
     1081 gcttcgtctc gggtctctgc catggcttat tgggacttct cttcggcgcg ttgggccttg
     1141 ccgccgctct tcggcgggcg ggggtcgaag gcgcggtggc cggttatggc ctggccgact
     1201 atcagggtgt tcacgtcgtt ggtcccctcg taggagtaga caccctcgat gtcggccatg
     1261 tgctccatca ctcgatagtc gaggaggacg ccattgcccc cgagcacgtc gcgtgccgtg
     1321 gcggcgatct ggcgcgcctt ggctacgttg ttcatcttga acatcgagac ggtcggcgca
     1381 tcgagctggc cggcgtcctt gaggcgtccg acgtggacgg agagtagctg gctaagcgag
     1441 atctcgttca gcatcatgac gagcttctgc tggacgagct ggaaggcggc tatgggctgg
     1501 ccgaactgca ccctctctcc ggcgtacgcc agggccgtct cgtagcaatc cgtggcctgg
     1561 ccgaggccgt cccagccgac cccgtagcgc gagtgcgtca ggaccgagag cgtggagccg
     1621 aggccctccg cccccggcag gcggttctcc tccgggatgc ggcaatcttt gagctctatg
     1681 tgcgtctgcc acgcggcgcg ctgggagccc ttgcgcgaga gtacctcggc gcggtagccg
     1741 gggttgtcgc cctcgacgag gaaacctgct atcttcccgt cttcggtgcg ggcccagatc
     1801 acggccacgt ccgcgaagga ggcgttgccg atccacttct tctcgccgtc cagaacgtag
     1861 ccgccgtccc tggctaccgc cgtagtggcg agggaaccag ggtccgagcc ggcctcgggc
     1921 tctgtgagac cgaagcaccc gaccttctcg cagcgggcca tcggcgggag ccacctctct
     1981 ttttgctcct ctgagccgag ctcgtggatc gccgtcatcg ccagcccgct ctggacgtgc
//
LOCUS       DASTUV010000337         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_240525, whole genome shotgun sequence.
ACCESSION   DASTUV010000337 DASTUV010000000
VERSION     DASTUV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240525"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFT03_06750"
     CDS             complement(<1..453)
                     /locus_tag="VFT03_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4847879.1"
                     /translation="MTQNGRLKVVNPATGEGIEEIETTSREDLDKAAQRARRGFKEWR
                     EVSGLERAELFHEIASNLRGHADELAGIMTKEGGKPFIENRDEVTWTAACFDYYGEIA
                     RDSVGYLPAPIEAQQLALVIKEPVGTVACIVPWNYPLLLAAWKVAPALA"
     gene            complement(457..1275)
                     /locus_tag="VFT03_06755"
     CDS             complement(457..1275)
                     /locus_tag="VFT03_06755"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847880.1"
                     /translation="MGESKGGPVYKGLGKIPDRALLLFAALIYVFLYAPILVLVFFSF
                     NSTKSTQVWTGFSTEWYWELINDQSVLEAFRLSMIVGVTATAIATVIGTLTALAMTRH
                     RFRGKTFADSAIYAATVMPEIVVGVSLLVFFVAAGIQLGIISILIAHVAFTISFVTIV
                     VRARLSGMDRSIEEAAQDLGANPIETFLRVTLPLILPGVMAGALLAFTLSLDDFVITF
                     FVAGVGSSTLPLKIYSMIKFGVSPVINALSTVVLVATMVLVFGGSRFFMRKEKS"
     gene            complement(1280..2146)
                     /locus_tag="VFT03_06760"
     CDS             complement(1280..2146)
                     /locus_tag="VFT03_06760"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4847881.1"
                     /translation="MGEEKGRGWSLGLAGLLTPVTVWLGLFFLVPLLFILAYSFGTSG
                     IYGGITLGFNPGNYLRVFDPLYLEIVGRTLVIALLNTFLCLLLGYPLAYFIAFKGGSR
                     KNLLILLVMIPFWTSLLLRAYAWVVILGGNGIANRALQFLGITDGPVNLIFTPQAVAL
                     GMVYSYLPFMILPLYAALEKFDIRLKEAAQDLGASRWHTFWRVTFPLSMPGVIAGSIL
                     VFIPSAGEFVIPNLLGGARTVLVGNLIQQQFLLARDWAFGSALAMMLAALLVGAIMFY
                     VRKVGTEKLEGV"
BASE COUNT          455 a          693 c          708 g          378 t
ORIGIN      
        1 ggctagcgcc ggggcgacct tccaggccgc taatagaaga gggtagttcc agggaactat
       61 gcacgcgacg gtgccgacgg gctcctttat gacgagcgcg agctgttggg cttcgatggg
      121 ggccgggagg tacccgacgg agtcgcgcgc gatctcgccg tagtagtcga agcaggcagc
      181 cgtccacgtt acctcgtcgc ggttctcgat aaagggcttg ccgccctctt tcgtcataat
      241 ccccgccagc tcgtcggcgt ggccacgcag attactcgct atctcgtgga agagctcggc
      301 ccgctcgagg cccgagacct cccgccactc tttgaagcct cgccgcgctc tttgcgcggc
      361 cttatcaagg tcctcgcgcg aagtggtctc tatctcttct atgccttccc cggtcgccgg
      421 gtttacgact ttcagacggc cgttctgcgt catgctctag gatttctcct tcctcataaa
      481 gaagcggctc ccgccgaaga ccaggaccat ggtggcgacg agcacgacag tcgagagcgc
      541 gttgatgaca gggctgacgc cgaacttgat catggagtag atcttcaagg gcagcgtgct
      601 ggatcccacc cccgcgacga agaacgttat gacgaagtcg tcgagcgaga gcgtgaacgc
      661 gagcagcgcc ccggccatga cgccgggtag gatcaggggt agcgtaaccc gcaaaaaagt
      721 ctctatgggg ttcgccccga ggtcctgggc cgcctcctcg atggagcggt ccatccccga
      781 gaggcgcgcc cgcaccacga tggtgacgaa cgagatcgtg aacgccacgt gcgcgatgag
      841 tatggagatg atgccgagct ggatccccgc ggcgacgaag aacaccagca ggctcacccc
      901 gacgactatc tcgggcatca cggtcgcggc gtagatcgcc gagtcggcga aggtcttgcc
      961 ccggaagcgg tggcgcgtca tggccagcgc ggttagcgtg cctatgacgg tagcgatggc
     1021 ggtggccgtc acccccacga tcatcgagag gcggaaggct tcgagtaccg actgatcgtt
     1081 gatgagctcc cagtaccact ctgtggagaa gccggtccag acctgggtgc tcttggtcga
     1141 gttgaacgag aagaacacga gcaccagtat tggcgcgtag aggaagacgt agatcagggc
     1201 cgcgaatagg agcagcgccc tgtcggggat cttgcccaac cccttgtaga cgggcccacc
     1261 cttcgactcg cccacggtcc tacaccccct ccagtttctc ggtccccact ttacggacgt
     1321 agaacattat cgccccgacc agcagagccg cgagcatcat cgccagcgcg ctgccgaagg
     1381 cccaatcgcg ggccagcagg aactgctgct ggatcaggtt ccctaccagc acggtccgcg
     1441 ctccgcccag cagattgggg atgacgaact cgccggccga ggggatgaag accaggatgc
     1501 tcccggctat gaccccaggc atgctcaagg ggaacgtcac ccgccagaag gtgtgccacc
     1561 ggctcgcccc gaggtcctgg gccgcctcct tcagcctgat gtcgaacttc tcgagggccg
     1621 cgtagagcgg caggatcatg aacggcaggt acgagtagac catccccaaa gccacggcct
     1681 gtggggtgaa gatgaggttc acgggaccat cggtaatacc gaggaactgc agcgcgcggt
     1741 tggcgatgcc gttgccgccc aggatcacca cccaggcgta ggccctcaag agcaggctgg
     1801 tccagaacgg gatcatgaca agcaggatca ggaggttctt gcggctaccg cccttgaagg
     1861 cgatgaagta agccaggggg taaccgagca ggaggcagag gaacgtgttg aggagcgcta
     1921 tgacgagcgt gcggcccacg atctccaggt aaagcgggtc gaagaccctc aggtagttgc
     1981 caggattgaa gccgagcgtg atcccgccgt agatcccgct ggtcccgaac gagtaagcca
     2041 gaatgaagag cagcggcacc aggaagaaca gcccgagcca gaccgtaacg ggggtgagca
     2101 ggcccgccag ccccagagac cagccgcgtc ctttctcttc tcccatgcta ggagctcttt
     2161 acttcggtga acagtttcga gtactcgcgg gtagccgcgc ccacgtcctc gatgatctgc
     2221 agacggtcgt acac
//
LOCUS       DASTUV010000338         6330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_930954, whole genome shotgun sequence.
ACCESSION   DASTUV010000338 DASTUV010000000
VERSION     DASTUV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930954"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFT03_06765"
     CDS             complement(<1..252)
                     /locus_tag="VFT03_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564156.1"
                     /note="catalyzes the phosphorylation of glucose using
                     polyphosphate or ATP as the phosphoryl donor; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4847882.1"
                     /translation="MNVFGIDVGGSGIKGAPVDADTGELLAERVRVKTPHPATSQAIL
                     ETAVEVVRQSGWDGPVGCGFPAVIKGGVVQTAANIDKSVI"
     gene            complement(350..1486)
                     /locus_tag="VFT03_06770"
     CDS             complement(350..1486)
                     /locus_tag="VFT03_06770"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU4847883.1"
                     /translation="MAISGALVVARQRGLSAVRANMEGALARLRVGSWPVVQTAVAAT
                     LAWSAAALVLGHEQPFVAAIAAVISVGAVAGQTLKRAAEWILGVAVGLTVADLIMLAI
                     GTGPVQTGVIVGLAMFAALLIRSGVMFVTEAGVSAVLVAGLDPTTSGVSPDRFLEALV
                     GGGAALAVSAVFPSNPSTRARQAARPVLEDLATALRNAAAGLIGGNLELAEAALSEAR
                     RIDAPVARLREELEGGYQIARLSPPRRRHLGHLGYYVAAADQLDLAARDTRVLARAAV
                     TLVREKGAAPGELAEAILGLALAVEALAGNLEQPGHTLDDARQFALGAAGEATSVLQK
                     SNDLETSALVAQIRSTAIDLLQAAGMESSEALEALREASRPSSG"
     gene            2012..3439
                     /locus_tag="VFT03_06775"
     CDS             2012..3439
                     /locus_tag="VFT03_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEU4847884.1"
                     /translation="MSAGSAGAGTGGQRTFVKVAAAITATGGLLFGYDTGVISGALLF
                     IKEDLGPLSDFVQGLIVSFLLVGAVVGALSGGPLTDRFGRRWTAMLAAIIFAIGALIV
                     ALTPNVGILIFGRFVLGLGVGLASLVVPLYIAEIAPPDTRGALVSLNQLMITIGILVS
                     YLVGVAFTPIEGWRWMFAVAIIPALVLGIGMFFLPETPRWLYENGFVERARAVLRRTL
                     AGDDAEVEREIEEIEKIRRVEEEQERVGYAELLKPFIRPALIIGIGLAIFQQITGINT
                     VIYYAPTTLEAAGFSAGGAIVATSLGVGVVNVGFTILSLYLIDRLGRRPLLLIGVAGM
                     VVSLFLLGLVFKLGADSPSLGILATVCLGLYIASFAISLGPVFWLMISEIYPLNVRGK
                     AMSVASLANWGSNFLVSLTFLLLISAFGQTGTFWLYAAIGVITWLFVYFLVPETKGRT
                     LEEIEADFRGTPVAPSDVAQSEEGA"
     gene            3457..4242
                     /locus_tag="VFT03_06780"
     CDS             3457..4242
                     /locus_tag="VFT03_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU4847885.1"
                     /translation="MGFRGAIFDVDGVLVDSPHEQAWREGLRQLMENDWSDIRDQTSY
                     APEKFTPEVYQRVMSGKPRYSGAQDALEYFGVPDAAIRAREYGDRKQVMIVELIEASQ
                     FHAYPDALRFVLAVKDMGLRIAAASSSKNANAFLERIRLDEFAEEEGLSYDFVRPGYS
                     LLDILDANVSGRDFEQGKPHPEIFLTAAEELGVPPEESFVVEDAANGVQAAKAGNMAA
                     LGLARADDEELLEEAGADIVVTSLDDVSLDALAEGRLERKDTS"
     gene            4412..5380
                     /locus_tag="VFT03_06785"
     CDS             4412..5380
                     /locus_tag="VFT03_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4847886.1"
                     /translation="MNAIGVDVGGTKIAAAVVSPEGKILNEVRYPTQAVPPNRLVETI
                     ARAITEVKDGFEVGGVCVAVPGLILAAENKVIFAPNLHEIENIRLDEELGPRTGLSVT
                     VENDANAAAWGEFRFGAGKDVDHQVFITLGTGVGGGVITHGVLLRGAQGAGGELGHVT
                     LDPTGPRCGCGNRGCLEALASGTAIGRRAREVANEKPRSALGQLAIERQVLGEDVTRL
                     AQEGDEAAISVLEETGRWLGIGLAGFVNIFNPEVIPIGGGASRAGEFILEPAREEVQL
                     RARSPSRDLVEIMLATLGPESGVLGAAALARSEDGEYVLGPSKRVA"
     gene            5395..6207
                     /gene="otsB"
                     /locus_tag="VFT03_06790"
     CDS             5395..6207
                     /gene="otsB"
                     /locus_tag="VFT03_06790"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU4847887.1"
                     /translation="MARHIGDLPHALEDGDELKRRLEGKRPAVFLDYDGTLTPIVDRP
                     EDALISESMRDAVRELAARCPVCVVSGRDRRVVQELMGIDDLIVAGSHGFDIWSPAGG
                     EVQREEGEEFAELLEGVEARLREELGAIDGALVEPKKSSVAAHYRLVSEDQRPEVRRA
                     VEEVLAGHPGELKVTPGKMVYEVQPNVDWDKGKAVLYLLVALDLDRDDVVPIYLGDDI
                     TDEHAFEALEARGIGVFVGRADDPEVGGRSTSADYVLNTFEEVERFLHTLAR"
BASE COUNT         1129 a         1969 c         2144 g         1088 t
ORIGIN      
        1 tatgaccgac ttgtcgatgt tggcagcggt ctgtacaacc ccgcccttga tcacagcagg
       61 gaaaccgcac cccacgggac catcccaccc ggattgcctc actacctcga cggcggtctc
      121 caggatggcc tgtgaagtag ccggatgcgg ggtcttgaca cgcactctct cggcgagcag
      181 ttcgccggta tccgcgtcca cgggcgcgcc ctttatcccg gagccgccga cgtctatgcc
      241 gaatacgttc atgcttcctc cagacccgta acctcgcctc tatcttattc taaaggacgc
      301 agcccccgag gctacccggg ggaggcgagg tctcactcga tggctcgaac tatcccgagc
      361 tggggcgcga ggcttcgcgg agcgcttcca gggcctcgga ggattccatg ccggcggctt
      421 gcagcaggtc tatggccgtc gatcggatct gggcgacgag cgcgctggtc tccaggtcgt
      481 tgctcttctg gagtaccgat gtggcctccc ccgcagcccc gagcgcgaac tggcgggcat
      541 cgtcgagcgt gtgaccaggc tgctccaggt tcccagccag ggcctccacc gcgagagcga
      601 gccccaggat cgcctcggcg agctctccgg gcgcggcccc tttctcgcgc accagcgtga
      661 cggcagcgcg agccagcacc ctggtgtcgc gcgctgcgag atcgagctgg tcggccgcgg
      721 cgacgtagta gccaaggtgc ccgagatgcc gcctgcgcgg cggggagagg cgggctatct
      781 ggtacccgcc ttcgagctcc tcgcgcagcc gcgctacagg agcgtcgatg cgccgcgctt
      841 cggagagcgc cgcttcggcc agctccaggt tcccgccgat caggccggcg gccgcgttcc
      901 gcaatgcggt ggccaggtcc tccagaacgg gccgcgccgc ctgcctcgcg cgcgtgctcg
      961 ggttactcgg gaacaccgcg ctcacggcca gggcagcacc accgcccacg agcgcctcga
     1021 ggaacctgtc cggcgacacc ccggaggtcg tgggatccag gccggccaca agcaccgccg
     1081 agaccccagc ctcggtgacg aacatgaccc cgctccggat caagagcgcg gcgaacatcg
     1141 ccagaccgac tatgaccccg gtctgaaccg gccccgtgcc gatcgcgagc atgatcaggt
     1201 ccgcgaccgt gagccctacg gcgacgccga ggatccattc cgcagcgcgc ttgagcgtct
     1261 gcccggcaac cgcgccgacc gagatcacag cagctatcgc cgccacgaaa ggttgctcat
     1321 gccccaacac aagcgccgcc gcagaccagg ccagcgtcgc cgctaccgcc gtctgcacga
     1381 caggccagct cccgacacgc aaccgcgcta gcgctccctc catgttggcg cgaaccgccg
     1441 acaaccctct ctgcctcgca acgaccagag caccagagat agccatcaca cctcccttcg
     1501 aacctcgctc gcttcgtcta tatgccaacg gcatacacca caccaatcta gcaccacttg
     1561 cgcggcaaac aagaggttcg agcgcgcttt ttgagcgtgt tgtcaaactg ttgctaaatc
     1621 gttaccaaac gttgccaggc tggtcgcgtg aggatcaggg cgtgtccgtt ggcgcggagg
     1681 atggcgctca cttcggcgca ggttctccat gatacggttg actcaaattt gccaccctgc
     1741 tagcgtccct tattacggga cgaagaaatg taccgctgtg caggacggta cgggaaggag
     1801 taatcgggga ttcagaaaaa ctgcggggga gtgccaggtg aagcggctcg atgcatgcag
     1861 gtgatttgcg ttgtatgttt caaaattctc tggtcagggc atacctacac gcacgcgagt
     1921 gggaaggaga agcggtagtc tccgaccagg acgctctgcg gatactgtgt tgggccatca
     1981 tgcggtctaa agaattggga agcgaggaaa tatgtctgcg gggagtgccg gcgcaggaac
     2041 aggtggccag cgaacgttcg taaaggtcgc ggctgcgatc acggcgacgg ggggcttgct
     2101 tttcggctac gacacgggtg tgatctcggg ggctttgctc tttataaagg aggacctcgg
     2161 gccgctctcg gacttcgtac agggtctgat agtcagcttc ttgctggtcg gagctgtggt
     2221 cggcgctctt tcgggcggac cgctcactga ccgattcggc cgcaggtgga ctgccatgct
     2281 ggctgccatt atcttcgcca tcggagccct gatcgtggcc ttgacgccca acgtgggaat
     2341 tctgatcttc ggccgctttg tgctgggttt gggcgtcggc ctggcctcgc tcgtcgtgcc
     2401 gctctacatc gccgagatag ccccgcctga cacgcgtggg gcgctggttt cgctcaacca
     2461 gctcatgatc acgataggga tcctggtctc ctacctggtg ggcgtcgcgt tcactcccat
     2521 cgaggggtgg cgctggatgt tcgcggtggc gataatccca gcgttggtcc tgggcatcgg
     2581 gatgtttttc ttgccggaga ccccacgctg gctttacgaa aacggcttcg tagagagggc
     2641 tcgcgccgtg ctgaggcgga cgctggccgg cgacgatgcc gaagtggaac gggagataga
     2701 ggagatcgag aagatccggc gcgtggagga ggagcaggag cgggtcggct acgcggagct
     2761 tctcaagccc tttattcggc cagccctcat aataggtata ggtctggcca tcttccaaca
     2821 gattaccggc atcaacaccg tcatctacta cgctccgact acgctcgagg ccgccggctt
     2881 ctcggccgga ggcgccatcg tcgccacctc tttaggcgtt ggcgtggtga acgtcgggtt
     2941 cacaatcctc tccctatatc tcatagacag gctgggacgc agaccgctgc tcctgatcgg
     3001 ggtcgctggc atggtcgtga gtctgttctt gctgggattg gtcttcaagc tcggggcaga
     3061 ttcgccctcg ctcgggatcc tggcgacggt gtgcctggga ctctacatcg cctccttcgc
     3121 gataagcctg gggccggtct tctggctgat gatctcggag atttacccct tgaacgtccg
     3181 cggcaaggcc atgagcgtgg cttccctcgc caactggggc tccaacttcc tcgtttccct
     3241 gacgttcctg ctgctcatca gcgccttcgg ccagaccggc acattctggc tgtacgcggc
     3301 catcggagtg attacctggc tgttcgtgta cttcctggtg cctgagacca agggccgaac
     3361 cctcgaggag atagaagccg acttcagggg cacgccggtc gccccgagtg atgtggctca
     3421 gtcagaagag ggagcctaga gcggaaagga gagcccatgg gttttcgtgg cgccatcttc
     3481 gacgtggacg gcgtcctggt cgactcgcca cacgaacagg cgtggcgcga gggtctgcgg
     3541 cagttgatgg agaacgattg gagcgacatc cgggaccaga cgtcttacgc tcccgagaag
     3601 ttcacgcccg aggtctacca gcgggtgatg tccggcaagc cgcgctactc gggcgcgcag
     3661 gacgcgctgg agtacttcgg ggtgcccgac gccgcgatac gcgccaggga gtacggcgac
     3721 cgcaagcagg tgatgatagt agagctgatc gaggcgagcc agttccacgc ctaccccgac
     3781 gctttgcgct tcgtgctggc ggtcaaggac atgggcctgc ggatagcggc ggcttcctcc
     3841 tccaagaacg ccaacgcttt tcttgagagg atccggctgg acgagttcgc cgaggaagag
     3901 ggcctgagct acgacttcgt gcggcccggc tactccctcc tggatatcct ggacgcgaac
     3961 gtcagcgggc gggatttcga gcagggcaag ccgcaccccg aaatcttcct gacggcggcg
     4021 gaggagcttg gcgtcccgcc ggaagagagc ttcgtcgtcg aggacgccgc gaacggcgtg
     4081 caggctgcga aggctggcaa catggcagcc ctcggcctcg cgcgggccga cgacgaggag
     4141 cttctcgagg aggcgggcgc cgacatagtc gtaaccagcc tcgacgacgt ctctctcgat
     4201 gcgctcgcgg aaggtcgcct ggagcggaaa gacaccagct aacggagcga cacatctttc
     4261 ctcgaccgta gcgggagact ctcgcccggg gttaacctga gaagagctct acgatagagg
     4321 cggccggagt gcggcgccgg tatgtccgtt cgggtaacgg gcgacgccga aggggtggag
     4381 caggagctcc ggagggacaa ggaggtaaag gatgaacgcc ataggggtgg acgtgggcgg
     4441 gacgaagatc gccgccgcgg tagtttcccc ggagggcaag atcctgaacg aggtcaggta
     4501 tccgacacag gcggtcccgc cgaaccggtt ggtagagacc atcgcgcggg ctatcacgga
     4561 ggtgaaggac ggcttcgagg ttggcggggt gtgcgttgcc gttcccgggc tcattctggc
     4621 cgccgagaac aaggtcatct tcgcgcccaa cctgcacgag atagagaaca tccgcctgga
     4681 cgaggagctg ggtcccagga ccggcctgtc ggtcacggtc gagaacgacg ccaacgcggc
     4741 ggcctggggc gagttccgct tcggagccgg caaagacgtc gaccaccagg tcttcataac
     4801 tctaggcacc ggggtcggcg gcggggtcat cacccacggg gtgctcctca gaggcgccca
     4861 gggagcgggc ggggagctgg ggcacgtgac cctcgacccc acgggaccgc gctgcggttg
     4921 cggcaaccgc ggctgcctcg aggcgctcgc ctccgggact gccatagggc gcagggcgcg
     4981 ggaggtcgcc aacgagaagc cacgctcggc gctggggcaa ctcgccatcg agcggcaggt
     5041 gctcggggag gatgtgacga ggctcgcgca ggaaggggac gaggcggcca tctcggtcct
     5101 ggaagagaca gggcggtggc tgggcatcgg cctggcgggg ttcgtgaaca tcttcaaccc
     5161 ggaggtgatc cccatcggcg gtggcgcctc gagggcggga gagttcatcc tcgaaccggc
     5221 ccgcgaggag gtgcaactca gggctcgttc cccttcacgc gaccttgtgg agataatgct
     5281 ggccaccctg ggcccggaat ccggcgtgct cggcgccgcg gcgctcgccc ggagcgaaga
     5341 cggggaatac gtgctgggtc cctccaagcg cgtggcgtga aagtggcgta gacggtggcc
     5401 aggcacattg gtgatctgcc gcacgcgctc gaggacggcg acgagctgaa aaggcgcctg
     5461 gagggaaagc gcccggccgt gttcctcgac tacgacggca cgctgacgcc gatcgtcgac
     5521 cggccggaag acgcgctcat ctcggagagc atgcgggacg ccgtccgcga gctggccgca
     5581 cgttgccccg tgtgcgtggt cagcgggcgc gacaggcggg tggtccagga gctcatgggc
     5641 atagacgacc tgatagtggc cggcagccac ggcttcgata tctggagccc cgctggcggc
     5701 gaggtccagc gcgaggaagg agaggagttc gcagaactgc tcgagggagt ggaggcgcgc
     5761 ttgcgcgagg agctgggcgc catcgatggc gccctcgtcg agccgaagaa gtcctccgtc
     5821 gccgcccact accggctcgt ttcggaagac cagcggccag aggtaagacg ggccgtcgag
     5881 gaggtcctcg ccggacaccc gggcgagctt aaggttacgc cgggcaagat ggtctacgag
     5941 gtccagccga acgtcgactg ggacaagggg aaggcagtcc tgtatctgct cgtcgcgctc
     6001 gacctcgacc gcgacgacgt ggtgccgata tacctgggcg acgacataac cgacgagcat
     6061 gcgttcgaag ctctggaagc cagaggcatc ggcgtcttcg tcggccgtgc cgacgacccg
     6121 gaggtaggcg ggcggtccac ctccgccgac tacgtgctca acacgttcga ggaggtggaa
     6181 cggttcctgc atacgctggc acggtgatca taagtatgaa gttctacggg gaggagaaac
     6241 tgaatgacga actggacgct cgcctacgac cgcttcgtgc ccgaggagga gggcttgcgc
     6301 gaggcgttga cctccaccgg caatggctac
//
LOCUS       DASTUV010000339         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_949621, whole genome shotgun sequence.
ACCESSION   DASTUV010000339 DASTUV010000000
VERSION     DASTUV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949621"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /gene="rpsS"
                     /locus_tag="VFT03_06795"
     CDS             <1..251
                     /gene="rpsS"
                     /locus_tag="VFT03_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU4847888.1"
                     /translation="EERLMERILRMNENNEKRMLKTWSRDSVIYPEFVGHTIAVHDGR
                     KHVPVYCTESMVGHRLGEFAPTRTFRTHVKSDRIARRR"
     gene            248..613
                     /gene="rplV"
                     /locus_tag="VFT03_06800"
     CDS             248..613
                     /gene="rplV"
                     /locus_tag="VFT03_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU4847889.1"
                     /translation="MRAKAKYVRIAPRKARLVADEVRGKSYPEAVSILQFTNKKGARL
                     IGDVISSAAANAEHNDGIDPEELFVREIRVDEGPIIRRYRARAMGRATMIRKRTSHIS
                     VELGAPVGVAVGTPEDEEE"
     gene            614..1270
                     /gene="rpsC"
                     /locus_tag="VFT03_06805"
     CDS             614..1270
                     /gene="rpsC"
                     /locus_tag="VFT03_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU4847890.1"
                     /translation="MGQKIHPEGFRLGVRRKGEKVDFKSSWYSDRDFAELLGEDLKIR
                     DHIEKKLSRAGIADIQIRKAAEQGEIAVDIHTARPGIVIGKGGSEVDALRSDLERLTG
                     KKVQVNVREVSRPELNAALVAESIAEQLEGRAAFRRTMKRALTSAMRSGAEGARIRCG
                     GRLGGIEMSREETISEGRVPLHTLDADIDYGFREAKTQMGQIGVKVWINHGVHEEGES
                     "
     gene            1270..1686
                     /gene="rplP"
                     /locus_tag="VFT03_06810"
     CDS             1270..1686
                     /gene="rplP"
                     /locus_tag="VFT03_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4847891.1"
                     /translation="MLVPKKLKYRKPHRGRMRGQAKGGTEVQFGEYGLQALEPAWITN
                     RQIEAARIAMTRKIKRGGKVWINIFPHKPVTKKPAETRMGSGKGSPESYVAVVKPGRV
                     MFEMSGVDEQLARDAMMLAAQKLPIKTRFLVRGGGQ"
     gene            1686..1883
                     /gene="rpmC"
                     /locus_tag="VFT03_06815"
     CDS             1686..1883
                     /gene="rpmC"
                     /locus_tag="VFT03_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU4847892.1"
                     /translation="MKAAEARELDVEELEQRLAETRRELFNLRFQHATGQLENTGQLT
                     EVRKNIARLLTVLNQKRQENG"
     gene            2033..2299
                     /gene="rpsQ"
                     /locus_tag="VFT03_06820"
     CDS             2033..2299
                     /gene="rpsQ"
                     /locus_tag="VFT03_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU4847893.1"
                     /translation="MQSDPDRGSRKERVGLVVSDRPEKTVTVSVETLVRHPMYKKRVR
                     RSKRFMVHDEGNEARVGDTVRIIETRPISARKRWRLANIVSRAE"
     gene            2299..2667
                     /gene="rplN"
                     /locus_tag="VFT03_06825"
     CDS             2299..2667
                     /gene="rplN"
                     /locus_tag="VFT03_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU4847894.1"
                     /translation="MIQQESRLKVADNSGARSLLTIRVLGGSKRRSAGVGDVVVASVK
                     EATPGGAVKKGDVVRAVVVRTKKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELREKGYTRIISLAPEVL"
     gene            2668..2985
                     /gene="rplX"
                     /locus_tag="VFT03_06830"
     CDS             2668..2985
                     /gene="rplX"
                     /locus_tag="VFT03_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU4847895.1"
                     /translation="MGLKIKRGDTVVVISGKEKGKRGEVERVLPRENRVVVGGVNVRT
                     RHARPTQNNQQGLYTFTAPIHVSNVKLVDPSSGQPTRVGYRFAGSGEKVRVAKGSGED
                     IDG"
     gene            2978..3520
                     /gene="rplE"
                     /locus_tag="VFT03_06835"
     CDS             2978..3520
                     /gene="rplE"
                     /locus_tag="VFT03_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4847896.1"
                     /translation="MARLKDRYDEEIAPALKERFGIENPMRIPRLEKIVVNMGVGEAS
                     QNSRALDGAIEDLAKITGQKAQMRRARKSIAGFKIREGMPVGARVTLRGERMWEFLDR
                     LVSVALPRVRDFRGVNPNAFDGHGNYALGLREQLIFPEISYDAIDTTRGLDVAVVTTT
                     ESDEEARELLRLLGMPFRDN"
     gene            3531..3716
                     /locus_tag="VFT03_06840"
     CDS             3531..3716
                     /locus_tag="VFT03_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU4847897.1"
                     /translation="MTRKALLVKQQKKQKYPVREYNRCQRCGRAHGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            3722..>3808
                     /gene="rpsH"
                     /locus_tag="VFT03_06845"
     CDS             3722..>3808
                     /gene="rpsH"
                     /locus_tag="VFT03_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4847898.1"
                     /translation="MSVNDPIADFLTRIRNAQMAKHDTAEIPH"
BASE COUNT          830 a         1105 c         1368 g          505 t
ORIGIN      
        1 tagaggagcg ccttatggag cgcatcctca ggatgaacga gaacaacgag aagcggatgc
       61 tcaagacgtg gagccgggac tcggtgatat atcccgagtt cgtcggccac accatcgccg
      121 tgcacgacgg gcgcaagcac gtccccgtct actgcaccga gagcatggtc ggacatcgcc
      181 ttggcgagtt cgctccgacg cggaccttcc gcacccacgt caagagcgac aggattgcga
      241 ggcgcagatg agggcaaaag ccaagtacgt ccgcatcgcc ccgcgcaagg cccgcctggt
      301 cgcggacgaa gtgcgcggca agagttatcc ggaagcggtc tcgatccttc aattcaccaa
      361 caagaagggg gcgaggctca tcggcgacgt cataagcagc gccgccgcca acgcggagca
      421 caacgacggc atcgacccgg aagagctctt cgtgcgggag atccgggtcg atgaggggcc
      481 gattataaga cgctacaggg cgcgcgccat ggggcgggcg accatgatcc gcaagcgcac
      541 aagccatatt agcgtcgagc tgggcgcgcc cgtgggcgta gcggtaggca cgcccgaaga
      601 cgaggaggaa tagatgggtc agaagataca cccggagggc ttccgcctcg gcgtcaggcg
      661 caagggtgag aaggtcgact tcaagagcag ctggtattcg gaccgggact tcgccgagct
      721 attgggggag gacctcaaga tccgcgacca catcgagaag aagctgagca gggccggcat
      781 agcggatatc cagatccgca aagccgccga gcagggcgag atcgcggtcg acatccatac
      841 cgcgcgcccc ggcatagtca tcggcaaggg cggtagcgag gtcgacgctc tgaggagcga
      901 cctcgagcgt ctcacgggca agaaggtcca ggtcaacgtg cgcgaggtct cgagacccga
      961 gctcaacgcc gcgctcgtgg ccgagtccat agccgagcag ctcgagggcc gggcggcttt
     1021 caggcgtacc atgaagcgcg cgctcacgag cgccatgcgc agcggcgccg agggggccag
     1081 gatcaggtgc ggcggtcgcc tgggcggcat cgagatgagc cgcgaggaga ccatctccga
     1141 ggggagagtc ccgctgcaca cgctcgatgc ggacatagat tacggcttca gggaggccaa
     1201 gacccagatg ggccagatcg gggtaaaggt ctggatcaac cacggggtgc atgaggaagg
     1261 tgagagctag tgctggtacc caagaagctc aagtaccgca agccccaccg gggccggatg
     1321 cggggccagg ccaagggcgg caccgaggtg cagttcggcg agtacgggct gcaggctctc
     1381 gagccggcct ggatcacgaa ccgtcagata gaggcggctc gtatcgcgat gacgcgcaag
     1441 atcaagcgcg gcgggaaggt ctggatcaac atcttcccgc acaagcccgt gaccaagaag
     1501 ccagccgaga cccggatggg cagtggcaag gggagccccg agtcctacgt cgccgtggta
     1561 aagcccggac gcgtgatgtt cgagatgagc ggcgtggacg agcagctggc gcgcgacgcc
     1621 atgatgctcg ccgcccagaa gctccctata aagacccgct tcctggtgcg aggaggtggc
     1681 cagtagtgaa agcggcggag gcacgcgagc tggacgtgga ggagctggag cagcgactgg
     1741 cggagacgcg ccgcgagctc ttcaaccttc gcttccagca cgcgacaggt cagttggaga
     1801 acacgggaca gcttacggag gtccggaaga acatcgcccg gctcctgacg gtcctgaacc
     1861 agaagaggca ggagaacgga tagtggtaga ggaagagagg aacagggagc aggctcccga
     1921 ggagagcccg gaggaggctg tagacgccct cgtcgagggc ggcgagacgg tcgaggccgg
     1981 cctcgacgcg ggaccggcca aaggccccgc gctcgaccag gacgagggca agatccagag
     2041 cgacccagac cgcggcagcc gcaaggagcg ggtcgggctc gtcgtctccg acaggcccga
     2101 gaagaccgtt acggtctccg tagagacgct tgtgcgccat ccgatgtaca agaagcgcgt
     2161 gaggcgctcc aagcgcttca tggtgcacga cgagggcaac gaggcgcgcg tcggggacac
     2221 ggtcaggatc atagagacca ggcccatctc cgcgcgcaag cgctggcggc tcgcgaacat
     2281 agtgtcgagg gccgagtagt gatccagcag gaatcaaggc taaaggtcgc ggacaattcg
     2341 ggggcgagga gcctcctgac catccgcgtg ctcggcggca gcaagcgccg gagtgccggc
     2401 gtcggagacg tggtcgtggc ttccgtcaag gaggctacgc ccggcggcgc ggtgaagaag
     2461 ggcgacgtcg tgcgggccgt ggtcgtgcgc accaagaagg agacgcgccg cgacgacggg
     2521 tcgtacatcc ggttcgggga gaacgcgggg gtaatcatca accccgacgg cgccccgcgc
     2581 gggacgcgca tcttcggtcc tgtcgcgcgc gagctgaggg agaagggcta cacgaggata
     2641 atctcgctgg ccccggaggt gctctagatg ggtctcaaga taaagcgcgg ggacaccgtg
     2701 gtggtgatct ccggcaagga gaagggcaag cgcggggagg tcgagcgggt tctcccgcgg
     2761 gagaaccgcg tggtcgttgg cggcgtcaac gtccgtacgc gccacgccag gccgacccag
     2821 aacaaccagc aggggctcta cacattcacg gcccccatac acgtttcgaa cgtgaagctg
     2881 gtggacccga gctcggggca gccgaccagg gtcgggtacc gcttcgcggg ctcgggggag
     2941 aaggtccggg tcgccaaagg atccggggag gacatagatg gctaggctga aggacagata
     3001 cgacgaggag atcgccccgg ctctcaaaga gaggttcggg atcgagaacc ccatgcggat
     3061 accacgcctg gagaagatcg tggtgaacat gggagtcggc gaggcgtcgc agaacagccg
     3121 cgcgctcgac ggcgcgatag aggacctggc gaagatcacg ggccagaagg cccagatgag
     3181 gcgggctcgc aagagcatcg ccggcttcaa gatccgtgag ggcatgccgg taggggccag
     3241 ggttaccttg cgcggcgaga ggatgtggga gttcctcgac cggcttgtct cggtggcgct
     3301 cccgcgcgtc agggacttca ggggggtcaa cccgaacgcc ttcgacggcc acgggaacta
     3361 cgccctgggc ctcagagagc agctcatctt ccccgagatc tcctacgacg ccatagacac
     3421 aacccgcggc ctcgatgtcg ccgtggtgac gacgacggag agcgacgagg aggcacgcga
     3481 gcttttgcgg ctgctcggga tgccgttcag ggacaattga gaggtagggt atgacgagga
     3541 aggctttgct ggtaaagcag cagaaaaagc agaaataccc ggtacgggag tacaaccggt
     3601 gccagcggtg cggccgggcg cacgggtact accgcaagtt cggactctgc aggatctgcc
     3661 tcagggaact cgcccacgag ggcaagatcc ctggagtaac gaaagcgagt tggtaggaaa
     3721 gatgtcggtt aacgatccca tagcggactt cctcaccagg atccgcaacg cgcagatggc
     3781 gaagcacgac accgccgaga tcccccac
//
LOCUS       DASTUV010000340         7044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1645298, whole genome shotgun sequence.
ACCESSION   DASTUV010000340 DASTUV010000000
VERSION     DASTUV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645298"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFT03_06850"
     CDS             <1..275
                     /locus_tag="VFT03_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847899.1"
                     /translation="EVWQSVRAILSDPERLRADLDAMIEMKRHNLYKSLRIGVLAHAD
                     GSTEIILGNLLSYEEVCTTETTSREPLLPTGDKLVYHRVSWMPGVR"
     gene            364..738
                     /locus_tag="VFT03_06855"
     CDS             364..738
                     /locus_tag="VFT03_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4847900.1"
                     /translation="MLDHVGVPVSDFERSKRYYAQALSPLGYELIMEPSASAAGFGRL
                     GKPDFWIAQGEIGQAFHIAFAAEDHATVDAFYEAALAAGGRDNGGPGLRPHYHASYYG
                     AFVLDPDGNNIEAVCHKPAEEA"
     gene            complement(831..1217)
                     /locus_tag="VFT03_06860"
     CDS             complement(831..1217)
                     /locus_tag="VFT03_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847901.1"
                     /translation="MDAAANHVVIVGGPEAEDELLAIVLDESEEAVPLFDSVEEAEEF
                     LDSIGEFGDDWRAREVSAQELIALLEYQGEEVEYVALSPPPERMEGGMEVQLLYREIL
                     IDLLRRQTATEPRSEERSFWRRLFGR"
     gene            1402..2481
                     /locus_tag="VFT03_06865"
     CDS             1402..2481
                     /locus_tag="VFT03_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847902.1"
                     /translation="MWLVSPFYDLAFIIFSSVLLIVPHLFSQLGGLSNVVVDLVVTAF
                     IGGPHLFATYTMTFMEPHFRERYRRYTWGALLMPVLVVTLAVVNLTLLVTIFFFWASV
                     HVIHQAAYIADSYRFKDPRGRAPDLARWQVVSRVIDYGLLMTSLYPIATFKFTGAPLV
                     IFGHNLSSHGFETGGRALLFPEFLKFEWLGPLAAAGFFAFLIAFIAKTLWEWRAGLLH
                     IPKTLHMSLAGLLFFVTPALPNLDVAFQGLNTWHSFQYLAVVLYLNQVRSERGLIGSQ
                     YVARVSSSGRRLYWMCFLFTLGAAVAYLLVLGANVALGTYGGNFSEQHYFAFYSVVLS
                     ALLIHYYFDHFLFLQVDDVITPRWT"
     gene            2593..3855
                     /locus_tag="VFT03_06870"
     CDS             2593..3855
                     /locus_tag="VFT03_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEU4847903.1"
                     /translation="MPDHTNPNHTMKGLAQKPERVSPGTDDVFDKAFKFTRAEEAMAA
                     GIYPYFKPISEQHGGIVEVEGREMIITGSNDYLGLTQDPRLEKAAVGVFDRYGTSCTG
                     SRSLTGTLALHEELERRLAGFFKAEATLTFTTGFLGMLAVLSALAGRRDILYLDRENH
                     ASLYDGARLSFGTMRKYRHNDLEDLERLLARDADKPGGRMIVTDGVFSMSGHIADLPG
                     IVELARRYGARVVVDDAHATGVLGETGRGTPEHFGLEDEVDLIIGTFSKSFASVGGFV
                     AGPRRVINYIKHHGRPFIFTAALPAMQVAVALEALEIMDTEPEHRERLTRNAEQLREG
                     IGSLGFDTLGSETQIVPVLIGPDELTVMFWKGLWEEGIFTTPALPPGVPRGQSIIRTS
                     VNANHTPEQIGRLLEAFATVGRGLGVIR"
     gene            3874..4989
                     /locus_tag="VFT03_06875"
     CDS             3874..4989
                     /locus_tag="VFT03_06875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4847904.1"
                     /translation="MGLTSIRTARSRADVKRFIRYPYSLYRDDPHWVPPLLIAEREQF
                     DPEKNPFYEHARVELFLAERDGEVVGRVAAIDDDNHNEIHDDNLIFFGFFEAEDQDAA
                     AALFTRVEERARELGRVAVRGPVNPSMNHSAGLLIDAFDSDPYLMMPYNPPEYPRYVE
                     EMGYRKVKDLYAWIFDRDWEVKKIGRLAERIRKRNEALVVRPVDMKRWDGELARVKDL
                     YNRAWERNWGFVRYTEAEFDQLAREFKMILDPELVALAEIDGELIGVTVLIPDANQVF
                     KKMRGRLLPFGVFHLLRRNKIMNRVRLPILGVAPEHRNKGFELVMIHDLYERAMARGY
                     KACEASWTLEDNRAMNHVIEAGGARRYKTYRLYEKEM"
     gene            4993..5958
                     /locus_tag="VFT03_06880"
     CDS             4993..5958
                     /locus_tag="VFT03_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4847905.1"
                     /translation="MPDRVLVTGATGFVGSHIAQAFVEAGYEVRVGARASSNERWISS
                     LDTERVALDLDGSAEDLSRAVHNVDVVVHAAGITRARRPGDYYSVNAGGTRSVATAAL
                     RAGVRRLVLISSLAARGPDGRDHPESDYGRSKLEAESHLRVLSGRMEAVVLRPAAVYG
                     PRDTDLLPLFRMARAGWLTVPSSAALLQPVYVQDAARATLAAAREPVGFGPFPVAENR
                     RYTWQDVTAGLEKALGRTVRAVRLPAGAFVLAGRVAEWAARPLSAVPILDERRSRDLA
                     LNAWTCDVSGTEEALGWRAEVPLFEGLERTARWYGQVGWLSMKSP"
     gene            5955..>7044
                     /locus_tag="VFT03_06885"
     CDS             5955..>7044
                     /locus_tag="VFT03_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4847906.1"
                     /translation="MSAAERRPEILFATIAAGGGHVAVARAMAEAVEHYYPGRFELRV
                     SDYMKEVGPKKVADLDRLHKDIWRFMLRYPILARGSQRLMDAFPRLTIATQRRLVRGF
                     ARAAADDLRRDPPRLIFSNHGLMTAGLAEAKRIGGLEVPVLSFANELVGICSYWADPH
                     ADHIVVPSEEAQRDLMRFGVPESKLSYVGFGYPVRQAFLDAPPRPEARTRLGLEDRFT
                     CLVSSGGEGVGRNQLALVRALLDSDVPLQVVVVAGRNEGLKKKLRALGTGKSLLRIEG
                     YVEDMATYLAASDVFVGKAGPASVYEAVVVGRPALVTGYAGLNEVGVARLVEERGLGR
                     YVRTPGELLQEVRRYALKPALLEEVARRC"
BASE COUNT         1217 a         2368 c         2224 g         1235 t
ORIGIN      
        1 ccgaggtgtg gcagtccgtt cgagcaatcc tctcagaccc tgagcgccta cgcgccgacc
       61 tggacgccat gatagagatg aagcgccaca atctctacaa gtccttgcgg ataggggttc
      121 ttgctcacgc cgacggctcc acggagatta tcctcgggaa cctactcagc tacgaagaag
      181 tttgtacaac ggaaacgaca tccagagaac cactgcttcc aacgggcgat aagctcgtgt
      241 accaccgagt ttcctggatg cctggcgttc gttgaatctc accgttgcgg aggggcgcgt
      301 gaccggggat actcggcttg acagatgtcg gttgggtcct gggggccgtc ggaggtgcga
      361 gcaatgctgg accacgtggg tgtacctgtc tcggatttcg agcggagcaa gcgctactac
      421 gcgcaagccc tctccccgct cggctacgag ctgataatgg agcccagcgc ctccgcggcg
      481 gggttcggta ggctgggcaa gccggacttc tggatcgccc agggcgagat aggccaggcc
      541 ttccacatcg cgttcgccgc cgaggaccac gccaccgttg acgccttcta cgaggcagcc
      601 ctggcagcgg gcgggcgcga caacggtggt cccgggcttc gcccccacta ccacgcgagc
      661 tactatggtg cctttgtcct agatccggac ggcaacaaca tcgaagcggt ctgccataag
      721 ccagcagaag aagcctgagg gtggtctcgc caacccgggg gagcctgttc gcgatgaaca
      781 agacagacgt catgtggcga cgctgcatga aagctcggcg ctcccgtgcc ctagcggccg
      841 aacaaccggc gccagaaact cctctcctcc gagcgtggtt cggtagctgt ctggcggcgc
      901 aggaggtcga tgaggatctc gcggtagagg agctggacct ccattccgcc ctccattctc
      961 tccggtgggg ggctcaaagc gacgtactcg acctcctcgc cctggtactc gaggagcgcg
     1021 atcagctcct gcgccgagac ctcgcgggcg cgccagtcat cgccgaactc accgatggag
     1081 tccaggaact cctcggcctc ctcaaccgag tcgaacagcg gcaccgcctc ttcggactcg
     1141 tcgagcacta tggcgagcag ctcgtcttcg gcctccggcc cgccgactat gaccacgtga
     1201 ttcgccgccg cgtccacacg tcaactctac tagatgtagc cgccaaaagt ccttgcaaag
     1261 gcgcggctgg cgtttataga ataacgcttg atgtgacgtg aaagttgcga gtctagagca
     1321 ctcgacccat tctccaggac ggtaagcgac ttgaccacct tgcctgcatc agcagccgcg
     1381 ccgagaatct cctccaagga aatgtggctg gtctcgccct tctacgacct cgccttcatc
     1441 atcttctcga gcgtgttgct gatagtgccg cacctgttct cccagctcgg cgggctctcg
     1501 aacgtcgtcg tcgacctggt cgtcaccgcc tttatcgggg ggccgcacct gttcgccacc
     1561 tacacgatga ccttcatgga gccgcacttc agggaacgct acaggcgcta cacgtgggga
     1621 gcgttgctca tgccggtgct cgtggtgacg ctcgccgtcg tcaacctgac cttgctggtc
     1681 accatcttct tcttctgggc ctctgtgcac gtcatccacc aggcggcgta catcgccgac
     1741 tcctaccgct tcaaggaccc gcggggccgc gcccccgacc tcgcccggtg gcaagtcgtc
     1801 tcgagggtca tagactacgg gctcctcatg acgagcctct accccatagc caccttcaag
     1861 ttcaccgggg cgccgctcgt gatcttcggc cacaacctct cgagccacgg cttcgagacg
     1921 ggagggaggg cgctcctctt ccccgagttc ctcaagttcg agtggctagg accgctcgcc
     1981 gcagccggat tttttgcgtt cctgatcgcc ttcatagcca agactctctg ggagtggagg
     2041 gcagggctac ttcacatccc caagacgctg cacatgagcc ttgccgggct actcttcttc
     2101 gtcacgccgg cgctgccgaa cctcgacgtg gccttccagg gcctcaacac ctggcactcc
     2161 ttccagtacc tggctgtcgt gctgtatctg aaccaggtgc gttccgagcg gggcctgatc
     2221 ggctcccagt acgtggcgcg ggtctcgagc agcggacgcc gcctctactg gatgtgcttc
     2281 ctgttcactc tgggcgccgc cgtcgcctac ctgctagtcc tcggtgctaa cgtggcgctc
     2341 ggaacctacg gcgggaactt ctcggagcaa cactacttcg cgttctactc cgtggtgctc
     2401 tcggcgctgc tcatccacta ttacttcgac cacttcctct tcctgcaggt ggacgacgtc
     2461 atcaccccgc gctggacctg agtactcggc gcttcctagt atcttctcca tgcgtgttct
     2521 atagttttgt tgtgacgcgc atacctcgtg taccctttat gatagttctg tctagctttg
     2581 tggagggctg gcttgccgga tcacacgaac cctaaccaca cgatgaaagg tctcgcgcag
     2641 aagccggagc gggtttcgcc aggcaccgac gacgtcttcg acaaggcttt caaattcacc
     2701 agggccgagg aggcgatggc cgccggaatc tacccttact tcaagccgat ctccgagcag
     2761 cacggcggca tcgtcgaggt ggaggggcgg gagatgatca tcaccggctc caacgactac
     2821 ctcgggctca cccaggaccc gcgcctcgaa aaagcggcgg tcggcgtctt cgaccgctac
     2881 ggcacgagct gtaccggctc gcgctccctt accggcacgc tcgccctgca cgaggagctc
     2941 gagcggcgcc tggccgggtt cttcaaggcc gaggcgacgc tgaccttcac caccggcttc
     3001 ctcggcatgc tggcggtcct gagcgctcta gccggccgcc gggacatcct ctacctcgac
     3061 cgcgagaacc acgccagcct ctacgacggg gcgagactct ccttcggcac gatgcgcaag
     3121 taccgccaca acgacctgga ggatctcgaa aggttgctcg ccagagacgc ggataagccg
     3181 ggcgggcgca tgatagtcac cgacggcgtc ttctcgatga gcggccacat cgccgacctt
     3241 cccggcatcg tggagctcgc ccgcaggtac ggggcgcgcg tcgtggtcga cgacgcgcac
     3301 gccacaggcg tgctcggcga gacaggcagg ggcacccccg agcacttcgg gctggaggac
     3361 gaggtcgacc tgatcatcgg gactttctcc aagtcgttcg cctccgtggg tggcttcgtg
     3421 gcgggcccga ggcgcgtcat caactatata aagcaccacg ggcgtccctt tatcttcacc
     3481 gcggcgctcc cggcgatgca ggtcgcggtg gcgctcgagg cgctcgagat catggacacg
     3541 gagcccgagc accgcgagcg gcttacccgg aacgccgagc agttgcggga ggggatcggc
     3601 tctctaggct tcgacacgct tggctccgag acccagatcg tgcccgtcct gataggccca
     3661 gacgagctga ccgtgatgtt ctggaagggg ctgtgggagg aaggcatctt cacgaccccg
     3721 gcccttcccc ccggcgtgcc ccgggggcag agcatcatcc gcaccagcgt caacgccaac
     3781 cacacccccg agcagatagg acgcctgctc gaggccttcg ccaccgtcgg ccggggcctt
     3841 ggcgtgatcc gctagaagga cccttcgggc ggcgtgggcc tcacgagtat ccggaccgcc
     3901 agatctcgcg ccgacgtgaa gcgctttatc cggtacccgt actcactcta ccgcgacgac
     3961 ccccactggg tccccccgct gctcatcgcc gagcgggagc agttcgaccc cgaaaagaac
     4021 cccttctacg agcacgcgcg ggtagaactc ttcctggccg agcgcgacgg agaggtcgta
     4081 gggcgcgtcg ccgccattga cgacgacaac cacaacgaga tccacgacga caaccttatc
     4141 ttcttcggct tcttcgaggc agaggaccag gacgccgccg cagccctgtt cacccgcgtc
     4201 gaagagcggg cgcgcgagct gggccgcgtt gcggtccgcg gccccgtcaa cccctcgatg
     4261 aaccactcgg cggggcttct catcgacgcc ttcgactccg acccttacct gatgatgccc
     4321 tacaacccgc cggagtaccc gcgctacgtc gaggagatgg gttaccgcaa ggtcaaggac
     4381 ctgtacgcct ggatcttcga ccgggactgg gaggtgaaga agatcggacg cctcgcggag
     4441 aggatccgca aacgcaacga agccctggtg gtccgacccg ttgacatgaa gcgctgggac
     4501 ggggagctcg ctcgcgtcaa agacctctac aacagggcgt gggagcgcaa ctggggcttc
     4561 gtgagatata ccgaggccga gttcgaccag ctcgccagag agttcaagat gatcctcgac
     4621 cccgagctcg tcgcgctcgc cgagatagac ggcgagctca taggggtcac cgtcctcatc
     4681 cccgacgcca accaggtctt caagaagatg cgcgggcggc tactcccgtt cggcgtcttc
     4741 cacctcctca gacgcaacaa gataatgaac cgggtgcgcc tgccgatact cggcgtcgct
     4801 cccgagcacc gcaacaaggg cttcgagctg gtcatgatcc acgacctcta cgagcgcgcg
     4861 atggccaggg gctacaaagc ttgcgaggcg tcgtggacgc tcgaggacaa cagggcgatg
     4921 aaccacgtca tcgaagcggg cggcgcgagg cgctacaaaa cgtaccgcct ctatgagaaa
     4981 gagatgtaga ccatgccaga ccgggtgctg gtcaccggcg cgaccggctt cgtcggcagc
     5041 cacatcgccc aggcgttcgt ggaggccggc tatgaggtaa gggtcggcgc gcgcgcctcc
     5101 agcaacgagc gttggatctc cagcctcgac accgagcgcg tggcgctgga tctggatggg
     5161 agcgcggagg acctgagccg ggccgttcac aacgtggacg tcgtcgttca cgcagcgggg
     5221 attacgaggg cgaggcggcc gggggactac tactcggtca atgcaggggg tactcgtagc
     5281 gtcgctaccg ccgccctccg ggcgggggtg cggcgcctgg tcctgatcag cagcctcgcc
     5341 gcgcgcggcc ccgacggccg cgaccacccc gagagcgact acggccggag caagctcgag
     5401 gcggagagcc acctccgcgt tttgagcggc cggatggagg ctgtagtcct ccgcccggcg
     5461 gccgtctacg gtccccgaga caccgacctc ctgcccctgt tcaggatggc gcgtgccggc
     5521 tggctgaccg tcccttcgag cgcggctctc ttgcaacccg tctacgtcca ggacgcggcc
     5581 cgcgccacac tcgctgcggc gcgcgagccg gtgggtttcg gtcccttccc ggtcgcggag
     5641 aacagacgct acacctggca ggacgtcacc gccgggctcg agaaggcgct cgggcgtacg
     5701 gtccgcgccg tacgcctgcc cgctggcgcc ttcgtgctcg cgggacgggt ggccgagtgg
     5761 gcggcgagac ctctctcggc cgtccccatc ctcgacgagc gcaggtcccg ggacctggcg
     5821 ctcaacgcct ggacctgcga cgtttccggg accgaggagg cgctcgggtg gcgggcggaa
     5881 gttcctctgt ttgagggtct ggagcgcacc gccaggtggt acgggcaggt ggggtggctt
     5941 agtatgaaat ctccatgagc gcggccgagc gcagacccga gatcctcttc gccacgatcg
     6001 ccgccggggg cgggcacgtg gctgtggcgc gcgccatggc cgaggccgta gaacactact
     6061 accccggccg cttcgagctg cgcgtctcgg actacatgaa agaggtcggc ccgaaaaagg
     6121 tcgccgacct cgaccggctg cacaaggaca tctggcggtt catgctgcgc taccctatcc
     6181 tggcgagagg tagccagcgg ctcatggacg ccttcccgcg cctgaccatc gccactcagc
     6241 ggcgcctcgt gcgaggcttc gcccgcgcag cggcagatga tctgagacgt gatcccccgc
     6301 ggctcatatt ctccaaccac ggtctcatga ccgcgggtct ggccgaagcc aagcgtatcg
     6361 gcggcctcga agtgccagtg ctcagcttcg ccaacgagct ggtcggcatc tgctcctact
     6421 gggccgaccc gcatgccgac cacatcgtcg ttccttcgga ggaggcgcag cgcgacctca
     6481 tgcgcttcgg agtgcccgag agcaagctgt cgtacgtagg cttcggctac cccgtccgcc
     6541 aggctttcct cgacgcaccc cccaggcccg aggcccgtac gcgcctcggg ctcgaagacc
     6601 gcttcacctg cctggtgtcg tccggcggcg agggggtggg gaggaaccag ctggcgctgg
     6661 ttcgggcgtt gctcgactcg gatgtccctt tgcaggtcgt agtcgtagcg ggcaggaacg
     6721 agggattgaa aaagaagctc cgggcgctcg ggacggggaa gagcctgctc aggatcgaag
     6781 gctacgtcga ggacatggct acttatctcg ccgcaagcga cgtcttcgtc ggcaaggcgg
     6841 ggccggcctc ggtctacgag gccgtcgtcg tcggtcgtcc cgcgctcgtc accggctacg
     6901 cggggctcaa cgaggttggt gtcgcgcggc tcgtcgagga gaggggactc ggacgctacg
     6961 tcaggacgcc gggggagctc ctgcaggagg tgcgtcgcta cgccctgaag cccgcgctcc
     7021 tcgaagaggt agcccgacga tgcc
//
LOCUS       DASTUV010000341         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_146678, whole genome shotgun sequence.
ACCESSION   DASTUV010000341 DASTUV010000000
VERSION     DASTUV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146678"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFT03_06890"
     CDS             <1..586
                     /locus_tag="VFT03_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4847907.1"
                     /translation="DPERGFRFSTYATWWIRQAVQRAVADKGRTIRVPVHMTEKIRKV
                     SRAVSELAVELERDPTEDEVAARLGWDPEEVRLTMSAMPDATSLDQPVSSEDTASQLG
                     DFIEDERVSDTPDTVMREMETDQLKEAIERLPDRARYVLVRRYGLDDREPATLAELGD
                     ELDISRERVRQLQREAERILKAGEYGRVLRDAVA"
     gene            674..1318
                     /locus_tag="VFT03_06895"
     CDS             674..1318
                     /locus_tag="VFT03_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4847908.1"
                     /translation="MKRGTQSWFWIYPLAFVLLSAVAGAHLLYPLDSWILRAVQSHAS
                     VTLDAAGTIFSVPGGAEYSGAAIILLTGGLLLAGRRCPAGRLLVAFVVTALLEFAMKT
                     WLPTVPVPDELARSTDPSFIEVSYPYPYPSGHMLRSVILFGAVYLLWPNRLLRAAILV
                     LLLGVAASRVYVGVHWPSDVAGGALLGVAGLAWVFKDPPNKRLQASAKNLKSET"
     gene            1337..2137
                     /locus_tag="VFT03_06900"
     CDS             1337..2137
                     /locus_tag="VFT03_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4847909.1"
                     /translation="MEITVVGSGTVVPRLERRQSCVVVEAGGETLVFDLGSGAVRGML
                     RARLDPFAVDRVFFTHFHPDHTVDLVTLLFTLKYGAPKQRTHPLHLTGPEPFERFWES
                     VTEAWGAWMVGDYPLEVSELPHVCPSPLQLSGTRLSWAPAEHRPESIAYRLDGAGGSF
                     VYTGDTEYSESVVELARGAHTMLVECSFPDESPVQGHMTPAAVARMASESGVRQIVLT
                     HIYPAADALDLPAEVGRGYNGEVVVAQDGSKLSVLNNSRNNDPSKNLE"
     gene            complement(2290..>2525)
                     /locus_tag="VFT03_06905"
     CDS             complement(2290..>2525)
                     /locus_tag="VFT03_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU4847910.1"
                     /translation="HELGIVRKEDFGEGYSRYELATERVHHHARCRECGAVIEFNEEL
                     MEYLALQVERETGFVSDWYEITLHGRCASCGDS"
BASE COUNT          437 a          827 c          813 g          448 t
ORIGIN      
        1 cgaccctgag aggggcttta ggttctcgac gtatgctacg tggtggatcc gtcaggcggt
       61 acagagggct gttgctgaca agggaaggac catccgggtc cccgtacaca tgacggagaa
      121 gatccggaag gtcagccgcg cggtcagcga gctcgccgtg gagctcgagc gtgatccgac
      181 cgaggacgag gtcgcggcgc gcctggggtg ggaccccgag gaggtacgcc tcacgatgag
      241 cgcgatgcct gacgccacca gcctggacca gccggtctcc tccgaggaca cggcttcgca
      301 gctcggcgac ttcatcgaag acgagcgcgt ctccgacaca cctgacacgg tgatgcgcga
      361 gatggagacg gaccagctca aggaggccat cgagcggctt ccggatcggg cgcggtacgt
      421 gctggtcagg cgctacggcc tcgacgatag ggagcccgcc accctcgccg agctgggcga
      481 cgagctcgac atctcgcgcg agcgggtcag gcagctacag cgagaggccg agcgtatcct
      541 caaggccggc gagtacggcc gcgttctgcg tgacgccgtg gcttaaccct tctaccccct
      601 tcgtaaatgt agccgggcct tcgggtccgg ctttttcgtg cccttctcgg cgctctttgc
      661 tagccttcga gacgtgaagc gaggaactca gtcgtggttc tggatctacc ccctggcctt
      721 cgtcctcttg tcggcggtgg ccggggcgca tcttctctac ccccttgact cctggatcct
      781 ccgcgcggta cagagccatg cctccgttac cctcgacgca gcggggacga tcttctcggt
      841 gccgggagga gcggagtact ccggagcggc tattatcctg ctcaccggcg gattgctcct
      901 cgcagggcgg cgatgcccgg cgggacggct cctcgtcgcg ttcgtggtca cggcgctgtt
      961 ggagttcgcg atgaagactt ggttgccgac cgttccggtg ccggacgaat tggcgcgctc
     1021 gaccgaccct tcgttcatcg aagtatctta cccctatccc tatccgagcg ggcacatgct
     1081 ccgttcggtc atcctgttcg gggccgtcta cctcctctgg cctaaccggc tccttcgcgc
     1141 agcgatcctg gtcctcctcc ttggcgtggc cgcgagccgc gtctacgtag gggtgcattg
     1201 gccctcggat gtggccggag gcgctctcct cggagtcgcg gggctggcgt gggtattcaa
     1261 agacccgcca aataaaaggc tccaggcatc agcgaagaac ctgaagtctg aaacctagtt
     1321 acgaagggaa gagtgcatgg agatcacggt cgtcggttcg ggcacggtag tcccccgcct
     1381 cgaaaggcgg cagagctgcg tcgtagtcga agccggcggg gagacactcg tgttcgacct
     1441 cggctcggga gccgtgcgag gtatgctgcg cgcgcgcctc gacccgttcg ctgtggaccg
     1501 cgtcttcttc acgcacttcc atccggatca cacggtcgac ctcgtcaccc tgctgttcac
     1561 cctgaagtac ggcgcgccaa aacaacgcac ccatcccctg cacctcacgg gtcccgagcc
     1621 cttcgagcgt ttctgggaat cggtgaccga ggcgtggggc gcctggatgg tgggtgatta
     1681 ccctctggag gtctccgagc taccccacgt gtgcccctct ccgctccagc tatcggggac
     1741 gcgcctctca tgggccccgg ccgagcacag gcccgagagc atcgcctacc ggctggatgg
     1801 cgcgggcggc tcgttcgtgt acaccggcga taccgagtac agcgagtcgg tcgtggagtt
     1861 ggcgcgcggc gcccacacca tgctcgtcga gtgctccttc ccggacgaga gtcccgtgca
     1921 gggtcacatg acgccggccg ccgtagcgag gatggcctcg gaatccggcg tcaggcagat
     1981 cgtactcaca cacatctacc cggcggcgga cgcgctggac ctccctgccg aggtggggcg
     2041 cgggtacaac ggcgaagtcg tagtggccca ggacgggtcg aagctcagcg tcctcaacaa
     2101 ttctcgaaat aatgatccca gcaagaacct ggaatagcca gcccctcaga gaccaacaag
     2161 ctgacaagtc gtctgaggcg aagccagggc gacgtgctga gaagcgacgc ccgaagggcc
     2221 gaagcgtgct gacaaggcgt ctggcgaagc cagcgccgtg ctgacaagcg aaagcgaagc
     2281 ctgagcgagc taagaatcgc cgcagctcgc gcagcggccg tgaagcgtta tctcatacca
     2341 gtccgagacg aagccagtct cacgctcgac ctgcaacgcc aggtactcca tgagctcctc
     2401 gttgaactct atgaccgccc cgcactcccg gcaacgggcg tgatggtgca cccgctccgt
     2461 cgcgagctcg tagcgcgagt acccctcgcc gaagtcttcc ttgcgcacga tgccaagctc
     2521 gtgca
//
LOCUS       DASTUV010000342         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1324844, whole genome shotgun sequence.
ACCESSION   DASTUV010000342 DASTUV010000000
VERSION     DASTUV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324844"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2467)
                     /locus_tag="VFT03_06910"
     CDS             complement(116..2467)
                     /locus_tag="VFT03_06910"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEU4847911.1"
                     /translation="MAETTRHTMGDRMPRKEDGRFIRGKGNYVDDVQLPGMLHSAILR
                     SPLAHAKINNIDVSAALELPGVHAVITGADLDAQGLAWMPTLAGDTQAVLATDKVRFQ
                     GQEVAFVVADDRYVAQDALELIEVDYEPLDVIVDSRKALEDGAPVIRDDKEGKEDNFI
                     FSWDAGDRDATEEAFQKAEVTVRQEMFYPRIHPAPMETCGAVADYDVSTGEMTVYMTS
                     QAPHAHRTLFAIVSGLPEHQIRIISPDIGGGFGNKVPIYPGYVCAAVASLVLGRPIKW
                     MESRAENLMSTSFARDYHMVGEIAATREGEILGVRSTILADHGAFNAHAQPGEQYPTG
                     FFPIFTSCYDVPAAYAEATGVYSNKAPGGIAYRCSFRVTEAIYLMERTMDVLADELGM
                     DPVELRRKNFVKKEQMPYVSVMGWEYDGGDYEGALQEALRIAGYEELLKEQEEARKEG
                     RIMGIGLSTFTEIVGAGPGKKCHIAGIEMFDSAELRFHPTGKAILKLGVKSQGQGHET
                     TFAQIVAGELGIPAEDVEVREGDTDNTPFGLGTYGSRSTPVCGAATAVVSRKIREKAK
                     KIAAHMLEAAEGDLEWTGERFQVKGSPDRGAGMAEIAFAAYTNVPEGEEAGLEGVTYY
                     DPPEMTYPFGAYICVVEIDEGTGRTEIKRFIAVDDCGVRINPTVVDGQIHGGLAQGIG
                     TALMEVIEFDDEGNHMNSNFMDYLTPTSMEVPNYELGEFVTPSTHHPLGARGVAESPT
                     VGSPAAVVNAVVDALSPYGVRHVDMPLTPNKIWSLLRENGEEA"
     gene            complement(2469..>2928)
                     /locus_tag="VFT03_06915"
     CDS             complement(2469..>2928)
                     /locus_tag="VFT03_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU4847912.1"
                     /translation="VNGEPREADVEPRLLLVHFVREYLGLTGTHWGCDTSNCGACTVH
                     LDGEPVKSCTVLAVRADGCEVTTIEGLWSGTELTPMQEGFHREHGLQCGFCTPGMIMT
                     GTALLEKNPDPTEAEIREALSGNLCRCTGYENIVKAVRWAAEANRTEVSA"
BASE COUNT          431 a          975 c          968 g          554 t
ORIGIN      
        1 gagggctcgg tgcggacgac cgtaacgacg gcgaagggct gctccgcctg cgcgagctcg
       61 ctcgcccgcc gcagcgagtc ggcgcgccgg gagaggtggt tggggtgagt agctttcagg
      121 cttcctcccc gttctcccgc aggaggctcc agatcttgtt cggcgtcagc ggcatgtcca
      181 cgtggcgcac cccgtaagga gagagcgcgt ccacgacggc gttgaccacc gcggcgggag
      241 acccgacagt cggagactcg gcgacgcccc gcgcgcccag gggatggtgc gtggaggggg
      301 tgacgaactc gcccaactcg tagttgggca cctccatcga ggttggcgtg aggtagtcca
      361 tgaagttgct gttcatgtgg ttgccttcat cgtcgaactc gataacctcc atcagggctg
      421 tgccgatgcc ctgcgcgagg ccgccgtgga tctggccgtc caccaccgtc gggttgatgc
      481 gcaccccgca gtcgtccacc gcgatgaaac gcttgatctc cgtcctgccc gtaccttcgt
      541 ctatctcgac gacgcagatg taggcgccaa acgggtatgt catctccggc gggtcgtagt
      601 acgttacgcc ctcgaggccg gcctcctcgc cctcggggac gttggtgtag gcggcgaatg
      661 cgatctcggc catgcccgca ccccggtcag gggagccctt gacctggaag cgctccccag
      721 tccactccag atcgccctcg gcggcctcga gcatatgggc ggctatcttc ttcgccttct
      781 ccctgatctt gcgggagacg acggcggtgg ccgcgccgca gaccggcgtc gagcggctgc
      841 cgtaggtccc gaggccgaag ggcgtgttgt ccgtgtcgcc ctcgcgcacc tcgacgtcct
      901 cggcggggat gccgagctct ccggctacga tctgggcgaa ggtcgtctcg tggccctggc
      961 cctgggactt gaccccgagc ttgaggatgg ctttgcctgt cgggtgaaac cgcagctcgg
     1021 cggagtcgaa catctctatc ccggcgatgt ggcacttctt gcctggaccg gcgcccacta
     1081 tctcggtgaa ggtggagagg ccgatgccca tgatgcggcc ttctttacgg gcctcttcct
     1141 gttccttcag aagctcctcg tagccggcga tccgcagggc ctcctgcagc gcgccctcgt
     1201 agtcgccgcc gtcgtactcc cagcccatga cggaaacgta gggcatctgc tccttcttga
     1261 cgaagttctt gcgccggagc tcgaccgggt ccataccgag ctcgtcggcg agcacgtcca
     1321 tcgtgcgctc catgaggtag atcgcctccg taacccggaa cgagcagcgg taggcgatgc
     1381 cccctggggc cttgttggag tagactccgg tcgcctccgc gtaagcagcg ggaacgtcat
     1441 aacagctcgt gaagatcggg aagaagcctg tggggtactg ctcgcccggc tgggcatggg
     1501 cgttgaaggc cccgtggtcc gcgaggatgg tcgagcgcac cccgagtatc tcaccctccc
     1561 tcgtggcggc gatctctccg accatgtggt agtcacgggc gaaggaggtg ctcatcaggt
     1621 tctcagcgcg cgactccatc cacttgatgg ggcgacccag tacgagtgat gctaccgcgg
     1681 cgcagacgta gccggggtag atcgggacct tgttcccgaa gcccccgccg atgtccggcg
     1741 agattatgcg gatctggtgc tccggcagcc ccgagactat ggcgaagagc gtcctgtggg
     1801 cgtgcggcgc ctgggaggtc atgtagacgg tcatctcacc ggtcgagacg tcgtagtcgg
     1861 cgacagcccc gcaggtctcc atcggggcag ggtggatgcg cggatagaac atctcctggc
     1921 gaaccgtgac ttcggctttc tggaacgcct cctcggtcgc gtctctgtct ccagcgtccc
     1981 aggagaagat gaagttgtct tctttgccct ctttgtcgtc ccggatcacc ggcgcgccgt
     2041 cttcgagcgc cttgcgggag tcaacgatca cgtcgagcgg ctcgtaatct acctctatga
     2101 gctccagggc gtcctgagca acgtaacggt cgtcggcgac gacgaaggcg acctcctggc
     2161 cctggaagcg caccttgtcg gtggcgagca cggcctgggt gtcgcccgcg agcgtcggca
     2221 tccaggcgag cccctgggcg tcgaggtcgg cgccggtgat gacggcgtgg acccctggca
     2281 gctccagcgc cgcggagacg tctatgttgt ttatcttcgc gtgggccagg gggctgcgca
     2341 ggatcgccga gtggagcatc ccggggagct gcacgtcgtc cacgtagttg cccttgcccc
     2401 ggataaagcg cccgtcctcc ttgcgcggca tccggtcgcc catggtgtgc ctcgtggtct
     2461 ctgccatctc aggcgctcac ctccgtcctg ttcgcctccg ccgcccaacg gacagccttg
     2521 acgatgttct cgtagccggt gcagcggcag aggttgccgg agagagcctc ccggatctcc
     2581 gcctccgtcg ggtcggggtt cttctcgagc agcgcggtgc ccgtcatgat catgcccggc
     2641 gtacagaacc cgcactgcag cccgtgctcc ctgtggaacc cctcctgcat tggcgtcagc
     2701 tcggtcccgc tccagagccc ctcgatggtc gtgacctcgc acccgtcggc ccggacggcc
     2761 aggacggtgc acgacttgac cggttcgccg tcgaggtgca cggtacacgc cccgcagttg
     2821 gacgtgtcgc acccccagtg ggtgccggtc aacccgagat actccctgac gaagtggacg
     2881 agcaggagcc gcggctcgac gtcggcctcg cgcggctcgc cgtttacg
//
LOCUS       DASTUV010000343         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_54139, whole genome shotgun sequence.
ACCESSION   DASTUV010000343 DASTUV010000000
VERSION     DASTUV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54139"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFT03_06920"
     CDS             <1..789
                     /locus_tag="VFT03_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847913.1"
                     /translation="LAPERFAADVSTGDLWLADPTEAFVLDPERLRGLAEVLAAVRRG
                     DVDGIVRALPLAGSFVPRDDSALRRELRETLGFLGGPLWREHSLREARDGGLEALRRG
                     DAKLHVEVALMARSLEEAEALGGGTKIVEAAEAAQVSISRYRDPAYVAARAARRLAQP
                     DTFADYPRQIHALLDELKDGEIEVRFRHAGLDDLISKVDILANRLVFGVIIAALILGS
                     SMLGIFVESGAQFLGLSVFGLIGFVIAALLGLLLLFAIIRSGRL"
     gene            1066..1545
                     /locus_tag="VFT03_06925"
     CDS             1066..1545
                     /locus_tag="VFT03_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU4847914.1"
                     /translation="MEDQIRSEILKTIPYGFYITGVVGSDGEVNGFTTCWVSQVSFDP
                     QQVIVAVRKDQHTHDLVEDSGVFSLNFLDTGQEDLARRFTEPLQHEDGLVGGSSYTTG
                     ETGAPLFEEAFAHLECRVSGKMEAGDHTVYLGDVVAANLRRPADILTDLDTPMEYGG"
     gene            complement(1776..1976)
                     /locus_tag="VFT03_06930"
     CDS             complement(1776..1976)
                     /locus_tag="VFT03_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847915.1"
                     /translation="MMHEEFAERFAERLEARADALEHGVRPTGQGATATRANELRTAA
                     AIAREEAAAVTEEEAPPPEAQA"
BASE COUNT          344 a          694 c          666 g          385 t
ORIGIN      
        1 ctcgcgcccg agcgattcgc cgcggacgtc tcgaccggcg atctctggct cgccgacccc
       61 acggaggcgt ttgtcctcga ccccgagagg ctgcgggggc tggcagaggt cctggcggca
      121 gtgcggcgcg gggatgtgga cgggatcgta cgcgccctac cgctcgccgg gagcttcgtc
      181 cctcgcgacg actccgctct ccggcgcgag ctgcgcgaga ctctgggctt cctaggcggg
      241 ccgctgtggc gcgagcactc gctgcgggaa gctcgtgacg gcgggctcga agcccttcgc
      301 agaggggacg cgaagctgca cgtcgaagta gcgctgatgg cgcgttcgct ggaggaggcc
      361 gaggcgctcg gaggcgggac gaagatagtc gaagcggcgg aggcggcgca agtgtccatc
      421 tcgcgttaca gggacccggc ctacgtcgcc gcccgcgcgg cacgccggct cgcccagccg
      481 gacaccttcg cagactaccc gcgtcagata cacgcgctcc tcgacgagct caaagacggc
      541 gagatcgagg tccgcttcag gcacgcgggg ctggacgacc tgatctccaa ggtggatatt
      601 ctcgccaacc ggctcgtctt cggcgtcatc atagctgccc ttatcctcgg ctcctcgatg
      661 ctcggtatct tcgtagagag cggggcccag ttcctgggct tgagcgtctt cggcctgatc
      721 gggttcgtca tcgcagcctt actgggtctc ctgctcctct tcgcgataat ccgctctggc
      781 cggctgtagg taagcacgct ccggcctagc ggcctccgcg tgtcagcacg ctcggccttg
      841 cggcctcgct tgtcagcacg gcgcttcgcg cctttcagca cgccgtctcc ctccggtcgg
      901 cgccttgtca gttttcgcct cgaatggtgc gggatctgtg cctttgagag aatatccagc
      961 taaggtgttg cggcttacgg tgcagagcct ccaccaaact cgtcaagctg aaatgctgac
     1021 aagcgacagc cgaaggctgg agcgtgctga tacaaggagc gcaacgtgga agaccagata
     1081 cgcagcgaga tcctcaagac gatcccttat ggcttctata tcacaggtgt cgtgggcagt
     1141 gacggcgagg tcaacggctt cacgacttgc tgggtttcgc aggtctcctt cgacccgcag
     1201 caggtcatcg tcgcggtgcg caaggaccag cacacgcacg acctcgtcga ggactccggc
     1261 gtattctcgc tgaacttcct cgacaccgga caagaagacc tggcccgcag gttcaccgag
     1321 ccgctgcagc acgaggacgg cctggttggc ggctcatcct acaccaccgg ggagaccggc
     1381 gcgccactct tcgaggaggc ctttgcccac ctggagtgca gggtgtccgg caagatggag
     1441 gccggggacc acaccgtcta tctcggcgac gtcgtcgcag ccaatctcag acgacctgcc
     1501 gacatcctca ccgacctgga taccccgatg gagtacggag gctaaccact cttatgggcg
     1561 ttagacagag cgggctaagc cagtagcgca accggctacc tcttgagtag agaggtcacc
     1621 cttctgaacg cagagcgccg gcccaggttg caagctggca cgaaagagcc gggccgacca
     1681 ggaggggggt aatttggtcg acccggcatg tgttgatcgt tgtgtgtacc ccgtcggtgc
     1741 cggactcaaa cctcggctgc ccaaccccgc ggggcttatg cctgcgcctc gggtggcgga
     1801 gcctcctcct cggtcaccgc cgcggcctcc tcccgcgcta tcgccgcagc cgtccgcaac
     1861 tcattggcgc gggtcgcggt ggcgccttgc cccgtcggtc gtactccgtg ttcgagggcg
     1921 tccgcgcggg cttcgaggcg ttcggcgaac ctctccgcga actcctcgtg catcatggtt
     1981 ccgatacccc cttcgtgtga agaccagtag tccgtgggtc aatcgaccct atctacttcc
     2041 ggttaaccgc cacgacgatg cgtggcgaac tcacacctta accgttcga
//
LOCUS       DASTUV010000344         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_748441, whole genome shotgun sequence.
ACCESSION   DASTUV010000344 DASTUV010000000
VERSION     DASTUV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748441"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1435)
                     /locus_tag="VFT03_06935"
     CDS             complement(257..1435)
                     /locus_tag="VFT03_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU4847916.1"
                     /translation="MLPLSGITVVSLEQAVAAPFATRQLADLGARVVKVERPEVGDFA
                     RGYDRTVKGLASHFVWLNRSKESVTLDLKQDGAKEILNRLIESADVFVQNLAPGATGR
                     LGFGAQTLRERHPSLIVCDVSGYGSSGPYRDKKAYDLLVQCEAGVVWITGTPDTPSKV
                     GVSIADIACGMYAYSGILAALFRRERTGEGAAIEVSLFEALAEWMGFPAYFTMYGGKE
                     PPRTGASHAAIAPYGPFECGDGEVIFLGIQNEREWERFCEAVLEQPALANDERFTSNS
                     ERVENRDDLHQEIEAILEELSSQEAIDRLDNAKIASARMRTVRDFLDHPQLEARGRWR
                     EVGSPVGPLRALLPPATMDGAEPVMAPIPTVGEHTDKILAELGYSDDAVASLRQSAAI
                     "
     gene            complement(1435..2592)
                     /locus_tag="VFT03_06940"
     CDS             complement(1435..2592)
                     /locus_tag="VFT03_06940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4847917.1"
                     /translation="MGRPDQYEGLRQGVREVCADFPDAYWRELDARREYPEAFIEAMT
                     KSGYLGALIPKEYGGLGLTLTETTIILEEINRSGGNAQPAHAQVYVMGTVLKHGSEEQ
                     KREYLPKIASGDLRLQAFGVTEPEAGTDTTSLRTTAVRDGDHYVVNGRKVYISRVLQS
                     DLMVLLARTTPLDRVSRKSEGLSVFLIDLREAIGDGLTVKPRRVMINNETNELFFEDL
                     RVPASSLVGEEGKGFRYILDGMNAERILIAAECIGNGRWFVDKATRRANERVVFGRPI
                     GQNQGIQFPLAQAYINVEAADLMRFRAAELFERGETCGAEANMALLLAAGASWEAANA
                     AMQTFGGYSFDAEYDVERKFRETRLFQVAPISTNLILSYIGEHVLGLPRSF"
     gene            complement(2594..>2782)
                     /locus_tag="VFT03_06945"
     CDS             complement(2594..>2782)
                     /locus_tag="VFT03_06945"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU4847918.1"
                     /translation="TSRVDVPKGDPENTLSREELEEKARRLAAFQGGASEKEMDRVIG
                     RVWDLDREPGVRDLLPEA"
BASE COUNT          468 a          911 c          862 g          541 t
ORIGIN      
        1 ctttcgacca gaggacggca cttggaaggt tgtccaccgg cacgcagacc ccataatcac
       61 ggctcagcct gccgagtcgg tactagagga gtagagctag cgccttagcg accttacacc
      121 caacgtccgg aattgtcatt ccacgagctt cgctggacag gcaggcgcgg acgtacgatc
      181 cccggatcta tctttgccga actcccgtcg aggggataag tgttgattca gatttggtgc
      241 gaagcccgat ggccgctcat atggcagccg actgccgcag ggacgctaca gcgtcatcgc
      301 tgtagccgag ctcggcgagt atcttatctg tgtgctcccc gacagtggga atcggggcca
      361 tgaccggctc cgccccgtcc atggtcgccg gcggaagcag cgcgcgcaaa ggacccaccg
      421 gggatcccac ctccctccac cggccgcggg cttcgagctg ggggtggtcg aggaagtccc
      481 tgacggtcct catccgggcg ctggctatct tcgcgttgtc gaggcgatct atggcctcct
      541 gagaagagag ctcctcgagg atggcttcta tctcctggtg caggtcatcc ctgttttcga
      601 cgcgctccga attgctggtg aagcgctcgt cgttagccag ggcgggctgc tccagcacgg
      661 cctcacagaa cctctcccac tcccgctcgt tctggattcc gaggaagatc acctccccgt
      721 cgccgcactc gaaggggccg taaggagcga tggctgcgtg gctcgccccg gtcctcggcg
      781 gttccttgcc accgtacatg gtgaagtagg cggggaagcc catccattca gcgagcgcct
      841 cgaagagcga cacctcgatg gcagcgcctt cgcccgtccg ctcgcggcgg aacaacgccg
      901 cgagtatgcc tgagtaggcg tacatgccgc aggcgatatc cgctatggag acccctacct
      961 tcgatggtgt atccggggtt ccggttatcc agacgacgcc ggcttcgcac tgcaccagca
     1021 ggtcgtaagc cttcttgtcg cggtacggtc cggaggagcc gtagccggat acgtcgcaga
     1081 cgatcaggga cggatgcctc tcccgcaagg tctgcgcccc gaagccgagt ctccccgtgg
     1141 cgcccggcgc gaggttctgg acgaagacgt cggcgctttc gatgaggcgg ttcagtatct
     1201 ccttggcccc gtcctgcttg agatccagcg taacggactc cttggaacgg ttgagccaga
     1261 cgaagtggct ggcgaggccc ttcacggtcc tgtcgtatcc gcgcgcgaag tcccccactt
     1321 ctgggcgttc gaccttaacg acacgggcgc ctaggtcggc gagctggcgg gtggcgaatg
     1381 gcgccgccac agcctgctcc agggagacga ccgttatgcc gctcagcggc agcatcagaa
     1441 agacctcggc agaccgagga cgtgctcccc tatgtacgag aggatgaggt tggtggagat
     1501 gggcgctacc tggaagagcc gggtctcgcg gaacttccgc tcgacgtcgt actctgcgtc
     1561 gaagctgtag ccgccgaagg tctgcatcgc cgcgttcgcc gcctcccagg aagcgccggc
     1621 cgcgagcagc aaggccatgt tggcttcggc gccgcaggtc tcgcccctct cgaacagctc
     1681 tgcggccctg aagcgcatca ggtctgcagc ctccacgttt atgtaggcct gtgccagagg
     1741 gaactggatg ccctggttct gtccaatcgg acggccgaag acgacgcgct cattggcacg
     1801 gcgggtagcc ttatccacga accaccttcc gttaccgatg cactctgcgg ctatgaggat
     1861 acgttcggca ttcatgccgt cgagtatgta gcgaaagcct ttgccttctt cgccaaccag
     1921 gctgctcgcc gggacgcgca ggtcctcgaa gaagagctcg ttggtctcgt tgttgatcat
     1981 cacgcgccgg ggcttcaccg tcagcccgtc gccaatagct tcgcgcagat ctatgaggaa
     2041 cacggagagc ccttcggact tcctacttac acgatcgagc ggcgtggtac gggccaggag
     2101 gaccatgagg tccgactgca ggactcggga gatgtagacc tttcgcccgt tgacgacgta
     2161 atggtctccg tctcgcacgg cggtcgtcct caaggaggtg gtgtctgtac cggcctccgg
     2221 ctcggtcacg ccgaaagcct gcaacctcag atcgccgcta gctatcttcg gcaggtactc
     2281 ccgcttctgc tcctcggagc cgtgcttgag cacagtgccc atgacgtaca cctgggcgtg
     2341 ggctggctgg gcgttgccgc cggagcggtt gatctcttcg aggatgatcg tcgtctcggt
     2401 gagggtcaga cccaaccctc cgtattcctt cgggatcagg gcgccgaggt agccgctctt
     2461 cgtcatcgcc tcgataaagg cttccgggta ctctcgtctc gcgtccagct cgcgccagta
     2521 ggcgtcaggg aagtccgcgc acacctcgcg gactccctgc cgcaagccct cgtactggtc
     2581 gggtcgtccc acgtcaggcc tccggcagca ggtctcgcac gccgggctcc cgatccaggt
     2641 cccagacgcg cccgatgaca cgatccatct ccttctccga agccccgccc tggaatgcag
     2701 cgagtctccg ggctttctcc tcgagctcct cacggctcaa cgtgttttcg gggtccccct
     2761 tcgggacgtc aacgcgggag gt
//
LOCUS       DASTUV010000345         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_733439, whole genome shotgun sequence.
ACCESSION   DASTUV010000345 DASTUV010000000
VERSION     DASTUV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733439"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..412)
                     /locus_tag="VFT03_06950"
     CDS             complement(119..412)
                     /locus_tag="VFT03_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847919.1"
                     /translation="MADRRTKERKLVALMVNDLGAESETDAAMIEDEAHALSDEELDR
                     TLARRLDLPYPVEEEVLERALARVEEPEELSEQGGFTGAPDMSHDPRPDEREG"
     gene            546..857
                     /locus_tag="VFT03_06955"
     CDS             546..857
                     /locus_tag="VFT03_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847920.1"
                     /translation="MHRAARSKMRSRAGAGSLAGLLSAKSLFSILSFGSVGSMLSIGC
                     VLSIGSAGSILSIGSAGSILSIGSTGSILSIGSVGSILGIGGAGSSPGSEREESEPDD
                     H"
     gene            complement(879..2645)
                     /locus_tag="VFT03_06960"
     CDS             complement(879..2645)
                     /locus_tag="VFT03_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEU4847921.1"
                     /translation="MRILNERREALVERERDLLERFIGFLKDFGAPSEDAELIRRAHA
                     DLEELFLLVIVGEFNSGKSAFINALLGAEIAGEGVTPTTDRITVLRHSDEPVERARRD
                     GILEKGHPNGFLQEVAVVDTPGTNAIIRHHEELSRGFVPRSDLVLFVTSAERPLTESE
                     RGYLELIRDWGKKVVLVVNKADLLRGEEDADEVRSFVESGIRSALGLAPPIFLVSALL
                     ARKAKAATSTIERDALLKASGFVDLERYVSELLDEEGRVRLKLESPLGVAEELARRYR
                     AAVEERLSLLEEDFKTSENVEAQLGLYTEDMRRDFEARLAEIENIIHSLNERGDAWLE
                     ENVRLMNFRELLRQEKVQERFKREVVADTGDLIDERVDELIDWMVDRNLKQWRAIVEY
                     VERRRQARYDDRLIGEVGDRFEYNRGQLLQSVGKNATGVVQHYDRDRESEQLAASIQG
                     AVAQTAALEVGAVGIGAVVVALATTRFLDATGLIAAAIIAGYGLFVLPNRRRKARRDF
                     REKTDSLRKRLGEVISRQFDAELGRSVERMRDAIAPYTRFVRTEHARMTEASSTLSGI
                     DGEIEALKEEIAAPGVGPGRAS"
BASE COUNT          445 a          934 c          890 g          530 t
ORIGIN      
        1 cggggcggac agcccgtaga gcagatacct ttcgcttgag tgtctactcc tcggccagga
       61 tgactctaca ggaggagttt ctccagaacg ttacgaggac cgttcggcgg agcaggaact
      121 agccctctcg ctcgtcgggg cgcgggtcgt gactcatgtc cggggcgcct gtgaagccac
      181 cttgttctga gagctcctcg ggctcctcga cgcgcgcgag ggcgcgttcc agcacctctt
      241 cttcgacagg gtagggcagg tcgagtctcc tggctagcgt cctgtcgagc tcctcgtcgg
      301 agagggcgtg tgcctcgtct tcgatcatcg ccgcgtccgt ttcgctctcg gcccccaggt
      361 cgttgaccat cagggctacc agcttccgct cctttgtcct ccgatcagcc atctctgcac
      421 cccgcggttc gtaagcttcc ggagcccaag tctacaacgc gagccactct ggcgggatgg
      481 gacacccggc tttgctgcta gactggctcg aggagccata atgaccggga agcgaggagg
      541 tcgtcatgca cagggctgca cgctcgaaga tgcgatcgcg cgccggggcg gggagcctcg
      601 cgggcctgtt gagcgcgaag agcctgttca gcatcttgag cttcgggagc gtgggcagca
      661 tgctgagcat cgggtgcgtg ttgagcatcg gcagcgcggg gtccatcctg agcataggaa
      721 gtgcgggttc gatcctgagc ataggcagca caggctccat cctcagcata gggagcgtgg
      781 gctcgatcct gggcatcgga ggcgccggat ccagccccgg ttccgagcgt gaagagtccg
      841 agccggatga tcactagaag gaccttcgag cacggaagct aggaagccct gcccgggccg
      901 acccccggcg cggctatctc ttctttgagg gcttcgatct caccgtctat cccggagagg
      961 gtcgagctag cctcggtcat acgcgcgtgc tccgtgcgta cgaagcgggt gtagggtgct
     1021 atggcatcgc gcatccgctc gacggagcgg cccagctccg cgtcgaactg cctgctaatc
     1081 acctcgccga ggcgtttgcg cagcgagtcg gtcttctcgc ggaaatcccg ccgcgccttg
     1141 cggcgccggt tcgggaggac gaagagaccg tagcccgcga ttatggcggc ggcgatcagg
     1201 cccgtcgcgt ctagaaaccg ggtcgtcgcg agggctacga cgacagcgcc tatgcccacg
     1261 gccccaacct cgagcgccgc cgtctgggcg acggcgccct ggatagaagc ggcgagttgc
     1321 tcggactctc ggtccctgtc gtagtgctgt acgacgcccg tggcgttctt gccgaccgac
     1381 tgcaggagct ggccacgatt gtactcgaag cggtcgccga cctccccgat cagtcggtcg
     1441 tcgtagcgcg cctggcggcg gcgctcgacg tattcgacga tggcgcgcca ctgcttgagg
     1501 ttcctgtcca ccatccagtc tatgagctcg tctacccgct cgtctatgag gtccccggtg
     1561 tcggcgacga cctcccgctt gaagcgctcc tgcaccttct cctgccggag gagctcgcgg
     1621 aagttcatca gccggacgtt ctcctcaagc cacgcgtcgc cccgctcgtt cagggagtgg
     1681 atgatgttct cgatctccgc caggcgcgcc tcgaagtcgc gcctcatgtc ctccgtgtaa
     1741 agcccgagct gagcctccac gttctccgag gtcttgaagt cctcttccag caggctcaaa
     1801 cgctcttcca ccgccgcgcg gtagcgtctg gcaagctcct cggccacccc gagcgggctc
     1861 tccagcttga gccgcactcg tccctcctcg tccagcagct ccgagacgta gcgctcgagg
     1921 tccacgaacc cgctcgcctt caacagggca tcgcgctcga tggtgctcgt ggcggccttc
     1981 gctttgcgtg ccaggagcgc tgagacgagg aagatgggcg gcgcgaggcc gagggcagac
     2041 cggatgccgc tctcgacgaa ggagcggacc tcgtcggcat cctcctcgcc gcgcagcagg
     2101 tcggccttgt ttacgaccag cacgaccttc tttccccagt cccgtatcag ctccaggtaa
     2161 ccgcgctcgc tctcggtcag gggacgctcg gccgaggtga cgaacaatac aaggtcgctc
     2221 ctcggcacga agccccggct cagctcctcg tggtggcgga tgatggcgtt cgtccccggc
     2281 gtgtccacga cggcgacctc ctgcagaaac ccgttcggat gccccttctc caggataccg
     2341 tctcgccgag ccctctcgac cggctcgtcg ctgtggcgca ggacggtgat gcggtccgtc
     2401 gtcggtgtca cgccctcgcc ggctatctcc gcgccgagca gcgcattgat gaaggcggac
     2461 ttccccgagt tgaactcgcc gacgatcacg agcaggaaga gctcctcgag atctgcatgc
     2521 gcccgccgga tgagctccgc gtcctcggac ggcgccccga agtccttcaa gaatcctatg
     2581 aacctctcca ggaggtctcg ctcccgctct accagagcct cccgccgctc gttgaggatc
     2641 ctcatctacc agacctccct tctcggcgca ttatagtagc gcacctccag ggccggtttc
     2701 gcggcaagcg cgtcgtggta aggaattggg agtattcggc aggctttatg agtggaggga
     2761 tgcgtgaact acaggaagct cggcaggacg gggctggag
//
LOCUS       DASTUV010000346         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_471492, whole genome shotgun sequence.
ACCESSION   DASTUV010000346 DASTUV010000000
VERSION     DASTUV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471492"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFT03_06965"
     CDS             complement(<1..614)
                     /locus_tag="VFT03_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847922.1"
                     /translation="MSRHSVIDVGSNTIHLLVGEVDGGAVLPVTGEKVSARLGAGVEK
                     TGRMAEERLSLAADAIALFAGISALNGVSEPVILATSAVRDAENGLELTERVRELTDL
                     KMRLISGEEEAALGFRGAVSAIGTSWEGPVLVVDLGGGSAQIIVGEASSGPLMQVSLP
                     LGSNRTTERFVNNDPAKKKELRALDEHVREMVPGWSLSQRVSVVA"
     gene            580..1119
                     /locus_tag="VFT03_06970"
     CDS             580..1119
                     /locus_tag="VFT03_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU4847923.1"
                     /translation="MDPTSITECRLKRSSFRVFVICVNCYTVHDTSPSIQVVYCAGST
                     PEEAISKKKAKKARARATGALTTLARPRPAAKVRDHDAHVLVCGSGDCKKRGSKEVRK
                     VLKEELRKRGMVGDVRVDSVDCLGLCKHGPNAVVYPGGTWYLGLTRSDVPEVVERHLE
                     GGEPVEHLAAGFRPRKKKR"
     gene            complement(1144..1725)
                     /locus_tag="VFT03_06975"
     CDS             complement(1144..1725)
                     /locus_tag="VFT03_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEU4847924.1"
                     /translation="MKKYKTQRSPAKMLAAVVSPLLIVAGLAVIGYSFMMGDPFATSA
                     LFGAEAPSNSTMKLTVPEMKRVDEVPVYDGGADDEAALHDGTLHVNGTGFPWQDGSNV
                     YIAGHRMGFPGTESYLVFWDLNVLENGDEVILTDANGTRYTYEVFKKTVVAPTDFHVT
                     QPVAGRSVVTLQTCTLPDYSDRLIVQAELTSVA"
     gene            1820..>2712
                     /locus_tag="VFT03_06980"
     CDS             1820..>2712
                     /locus_tag="VFT03_06980"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847925.1"
                     /translation="MVTVAAGEREQALASIISGVKIGVPREIAEGEERVGLVPETVGR
                     LVKEGFEVLVETGAGRDYNLDENYEEAGATIVEDAGKVYGEADLVVKVASPTEEEVGL
                     MQEGQILLCFLNGPQHPGLVKKLADAGVTVFSNEAIPRTSVAQSMDALSSMGSIAGYK
                     CALIGANALGKYVPMLSTAAGTTKAAKILVFGVAVAGLQAIAMTNRLGGEVFAYDIRP
                     ETKEQAESLGASFIDSNDEKEDGAGEDEFVEYKPTGFRKLMSSLGFYSFAEPPRDEYV
                     VEGEEEPEEDSEEEEGWSEEKL"
BASE COUNT          541 a          880 c          834 g          457 t
ORIGIN      
        1 gccacgaccg agacccgctg cgataggctc caccctggca ccatctccct gacgtgctca
       61 tcgagcgccc gtaactcctt tttctttgcc ggatcgttgt tcacgaatcg ctccgtagtg
      121 cggttggagc caagcggcag cgagacctgc atgagcgggc cgcttgacgc ctccccgacg
      181 ataatctgcg ccgatccccc gccgaggtcc accacgagta caggcccctc ccacgaagtc
      241 ccgatcgccg acaccgcccc ccgaaatccg agcgcggcct cctcttcgcc cgagatcagg
      301 cgcatcttca gatccgtaag ctcccgcacc ctctccgtta gctccaggcc attctccgcg
      361 tctcgcaccg cgctcgtggc aaggatcacc ggctcggaaa cgccgttcag cgcagagatc
      421 ccggcgaaga gggcgatggc atccgccgcc agcgatagcc tctcctccgc catgcgcccc
      481 gtcttctcca ctccagcccc gagacgggcc gagaccttct cgccggtgac gggtagaacc
      541 gcgcccccat ccacctcccc aacgaggaga tgtatggtgt tggatcccac gtctatcacc
      601 gagtgccgac tcaagcggtc atccttccgg gtgttcgtga tctgcgtgaa ctgctacacc
      661 gtccatgata catccccatc catccaggtg gtatattgcg ccggatcaac accggaggaa
      721 gccatctcga agaagaaagc gaagaaggcc agagcccgcg cgaccggagc cctgaccacc
      781 ctggcgcggc cgagaccagc agcgaaagtc agggatcacg atgcccacgt gctcgtgtgc
      841 ggcagcggcg actgcaagaa gcgcggctcg aaagaagtcc gcaaggtcct gaaagaagag
      901 ctgcgcaaga ggggtatggt cggggacgtc cgcgtggact ccgtcgattg tctcggcctc
      961 tgcaaacacg gcccgaacgc cgtcgtctac cccggaggca cctggtacct cggcctcacg
     1021 agaagcgacg tgcccgaggt cgtcgaacgc cacctcgagg gcggcgagcc cgttgaacac
     1081 ctcgccgccg ggttccgtcc ccgcaagaag aagaggtagg ggcggttcgc gaaccgctct
     1141 acgctaagcg acactcgtca gctcggcctg gacgatcagg cggtccgagt agtcgggcag
     1201 cgtacacgtc tgcagggtga ccacgctcct gccggcgacc ggctgcgtga cgtggaaatc
     1261 ggttggggct acgacggtct tcttgaagac ctcataggtg tagcgcgtgc cgttggcgtc
     1321 ggtcagaatg acctcatcac cgttctcgag cacgttcaga tcccagaaga ccagataact
     1381 ctcggtgccg gggaaaccca tgcggtggcc cgctatgtag acgttggatc catcctgcca
     1441 cgggaagccc gtgccgttga cgtggagcgt cccgtcgtgg agcgcagcct cgtcgtcagc
     1501 gccgccgtcg tagacgggca cctcgtcgac gcgcttcatc tctgggacgg ttagcttcat
     1561 cgtcgagttg ctcggggcct cggcgccgaa gagggccgag gtcgcgaaag ggtcccccat
     1621 catgaaggaa tacccgatga cagccagccc ggcgacgatg aggagaggcg agacgaccgc
     1681 cgccagcatc ttggccgggc tacgctgcgt cttgtacttc ttcatcgccc tcctcctgcc
     1741 ttctcacaca acgctttcaa ttcctcaaat ataaacatag cccttaaacg taacataccg
     1801 gttacaaaat ggggtcttgt tggtaacggt cgccgccggt gagcgcgaac aagcactcgc
     1861 gtctataatc tccggcgtga agataggggt tcctagggag atagctgaag gcgaggagcg
     1921 ggttgggctc gtacccgaga ccgtcggcag gctcgtcaag gaagggttcg aggtcctcgt
     1981 cgagaccgga gcgggccgcg actacaacct cgacgagaac tacgaggagg ccggagccac
     2041 gattgtcgag gacgccggca aggtatacgg agaggctgac ctcgtcgtca aggtcgccag
     2101 ccccaccgaa gaagaagtgg gtctgatgca agagggccag atccttctct gcttcctaaa
     2161 cgggccgcag catcccgggc tcgtcaagaa gctcgccgac gccggcgtga cggtcttctc
     2221 aaacgaggcg atcccgcgca cgagcgtcgc gcagagcatg gacgcgctct cctcgatggg
     2281 ctccatcgcc ggctacaaat gcgcgcttat cggggccaac gctctgggca agtacgttcc
     2341 catgctctcg accgccgccg ggactacgaa agcggcgaag atcctggtct tcggagtggc
     2401 tgtcgcgggc ttgcaggcta tagcgatgac gaaccgcctc gggggagagg tcttcgccta
     2461 cgacatacgc cccgagacga aggagcaggc cgagtccctg ggcgcttcat tcatagactc
     2521 caacgatgag aaagaggacg gcgccgggga ggacgagttc gtcgagtaca agcctaccgg
     2581 cttccgcaag ctgatgtcct ctctgggctt ctactccttc gccgagccgc ccagagacga
     2641 gtacgtcgtc gagggcgagg aggaacccga ggaagactcc gaagaagagg agggctggtc
     2701 ggaggagaag ct
//
LOCUS       DASTUV010000347         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_850476, whole genome shotgun sequence.
ACCESSION   DASTUV010000347 DASTUV010000000
VERSION     DASTUV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850476"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFT03_06985"
     CDS             complement(<1..1076)
                     /locus_tag="VFT03_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU4847926.1"
                     /translation="MSTLKLPPGPKGRPVLGNMLGYARDPLGFLTRCSREHGDVVRLR
                     FPGTLAYLITHPDDVEQVLVKNNRNFIKDKYTREELSVLGNGLLINEGDFWRRQRRLA
                     QPAFHRRRVEAYGETMVAFTERMLEGWQDGEVRNVHTEMMRLTLEIVAKTLLDADIAG
                     EAEGVGEALGQIMDYFSDQGSGSFLRLLPESVPTPANVRYRRAVRRLDGIIYSIIEER
                     RRSGADTGDLLSMLLHAEDEDGNRMDPKQLRDEVMTVVLAGHETTAIALSWTFYLLGE
                     NPETERNLTAELEEVLDGRAATVRDLSRLRYADAVIKESMRLYPPAWAIGREALGDCE
                     IGGYHVPTKTQMFISQYVTHRDPR"
     gene            complement(1081..1398)
                     /locus_tag="VFT03_06990"
     CDS             complement(1081..1398)
                     /locus_tag="VFT03_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU4847927.1"
                     /translation="MDATQDNEQQSAHGYIKARDKELLQNRLKRIEGQVRGVQRMVDE
                     EAYCVDVLTQIASVVSALEKVGTILLKDHVEHCVRESIENGEDAEEKIEELTAAVERF
                     LRV"
     gene            complement(1415..>2912)
                     /locus_tag="VFT03_06995"
     CDS             complement(1415..>2912)
                     /locus_tag="VFT03_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEU4847928.1"
                     /translation="EKVPVDGVVVSGESAVDEAMISGESMPEVKRVGDEVIGATINTT
                     GSFRFEATKVGKDTVLSQIVKIVEEAQGTKAPMQRLADRISGVFVPVVVAIAALTFVA
                     WLLLGPEPAFALALLNFVSVLIIACPCAMGLATPTSIMVGTGKGAESGILIKGGEALE
                     AAHKLTTVVLDKTGTLTRGEPELTEVVATNGLGEDEVLRLAASAERTSEHPLGEAIVR
                     GAKDRGLSLAEAEEFGATSGGGVEALVEGHAIRVGSRRFLAGSGADQDELGQPGEELA
                     REGKTHVFVTVDGEPAGIVAVADTVRSESREAVARLREMGLEVAMITGDDSRTAGGIA
                     RELDIDRVLAEVLPREKAAEVKRLQKEGEKVAMVGDGINDAPALAQADVGIAIGTGAD
                     VALEAADLTLISGDVRGVARAIKLSKATVRNIKQNLFWAFAYNVALIPVAAGVLYPFF
                     AGGAVPEVLRPVLGEYGFLNPVLAAAAMALSSVTVVSNALRLRRMKVD"
BASE COUNT          441 a         1010 c          900 g          561 t
ORIGIN      
        1 aagcgggggt cgcggtgcgt cacgtactgg ctgatgaaca tctgggtctt cgtagggacg
       61 tggtagcccc cgatctcaca gtctccgaga gcctcccggc cgatggccca ggccggcgga
      121 tagaggcgca tggactcttt gatgaccgca tcggcgtagc gcagccggga caggtcccgg
      181 acggtcgcgg cgcgtccgtc gagcacctct tccagctcgg ccgtgaggtt tctctctgtc
      241 tccgggttct cgccgagcag gtagaacgtc cacgaaaggg ctatggcggt tgtctcgtga
      301 cctgcaagga cgacggtcat gacctcgtcc ctgagctgtt tggggtccat cctgttgccg
      361 tcttcgtctt cggcgtggag cagcatggag aggaggtcgc cggtgtccgc gccgctgcgc
      421 cggcgctctt cgatgatgga gtagatgatc ccgtcgagcc gccgcacagc tctgcggtag
      481 cgcacgttgg ccggggtggg gacgctctcg gggagcaagc gcaagaacga gccgcttccc
      541 tgatctgaga agtagtccat tatctgtccg agagcctctc cgacgccctc ggcctcgccc
      601 gctatgtcgg cgtcgagcag ggtcttggcg acgatctcga gggtgaggcg catcatctcc
      661 gtgtggacgt tgcggacctc gccgtcctgc cagccctcga gcatccgctc ggtaaaggcc
      721 accatcgtct cgccgtaggc ctctacgcgc cggcggtgga aggctggctg ggcgagccgc
      781 ctctggcggc gccagaagtc gccctcgttt atcaggagcc cgttgccgag gacgctcagc
      841 tcctcgcggg tgtatttgtc cttgatgaag ttgcggttgt tcttcaccag cacttgctcg
      901 acgtcgtcgg ggtgggtgat caggtacgcc agcgtacccg ggaagcgcag cctgacgacg
      961 tcgccgtgct cgcgcgagca ccgcgtcaag aacccaagag ggtccctggc gtaacccagc
     1021 atgttgccga gtaccggacg ccccttcggg cccggcggaa gcttcagggt cgacaacgcc
     1081 ctacaccctc aagaagcgct cgacggcggc tgtgagctct tcgatcttct cctcagcgtc
     1141 ctcgccgttc tctatggact ccctgacgca gtgctcgacg tggtccttga gcaggatggt
     1201 gccaaccttc tctaaagccg agacgacgct tgcgatctgg gtcaggacgt ccacgcagta
     1261 ggcttcctcg tctaccatcc gctgcacgcc cctcacctgc ccctcgatcc gcttgaggcg
     1321 gttctggagc aactctttgt cccgcgcctt tatgtagccg tgcgctgatt gctgctcgtt
     1381 gtcctgcgtc gcgtccatct ctctatcctt cctgctagtc aaccttcatc ctgcgcagcc
     1441 tgagggcgtt gctaaccacc gtcacgctcg agagcgccat cgccgccgcc gcgagcacgg
     1501 ggttgaggaa tccgtactcc ccgagaaccg gcctcaacac ctcaggcacc gcgccgcccg
     1561 cgaagaacgg gtagagtacc ccggccgcta ccgggatgag ggcgacgttg taggcgaacg
     1621 cccagaagag gttctgcttg atgttcctca cggtggcctt cgagagcttg atggcccgcg
     1681 cgaccccccg cacgtcgccg gagatcaggg tgaggtcggc agcctcaagc gccacgtccg
     1741 cgccggtgcc gatggcgatc ccgacgtctg cttgggccag cgccggagcg tcgttgatgc
     1801 cgtctccgac catggcaacc ttctcgcctt ctttctgaag ccgcttcacc tcggcagcct
     1861 tctcacgcgg caacacctcg gcgagaactc tgtctatgtc gagctcgcgg gctatgccac
     1921 cggcggtccg gctgtcgtcg ccagtgatca ttgctacctc gagccccatc tcgcgcagcc
     1981 tcgcgaccgc ctcgcgcgat tcgctcctga ctgtgtcggc caccgcgact atgcccgcgg
     2041 gctccccgtc caccgtaacg aatacgtgcg tcttgccctc acgcgcgagc tcctcacccg
     2101 gctggccgag ctcgtcctgg tcggcccccg acccggccag gaacctccgg ctcccgacgc
     2161 ggatagcgtg gccctccacc aaggcttcca caccgccgcc gctcgtggcg ccgaactcct
     2221 cggcctctgc gagggagaga ccccgatcct tcgcacccct cacgatggcc tcgcccagag
     2281 ggtgctcgct ggtcctttcg gctgaagcgg cgaggcgcag gacttcgtct tcgccgagac
     2341 cgttggtggc gaccacctct gtcagctcgg gctcgcccct ggtgagggtg ccggtcttgt
     2401 cgagtacgac tgtggttagc ttgtgggcgg cttcgagagc ttcgccgccc ttgatgagga
     2461 tgccggattc tgcgcccttg cctgtgccga ccattatcga ggtcggggtc gcaagcccca
     2521 tcgcgcacgg gcaggcgatg atcaggaccg agacgaagtt gaggagcgcg agcgcgaagg
     2581 ccggctcggg gccgagaagg agccacgcca cgaacgtgag ggccgcgatc gccaccacca
     2641 ccggcacgaa gacgccgctt atcctgtccg caagccgctg catgggcgct ttcgtgccct
     2701 gcgcctcctc gactatcttc acgatctggg agagaacggt gtccttcccg accttcgtgg
     2761 cctcgaagcg gaacgagccg gtggtgttta tcgtcgcccc gatgacctcg tcgcctaccc
     2821 tctttacttc aggcatggac tcgccggaaa tcatagcctc gtccaccgcc gactcgcccg
     2881 agaccaccac gccatcgacc ggcaccttct cc
//
LOCUS       DASTUV010000348         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_859459, whole genome shotgun sequence.
ACCESSION   DASTUV010000348 DASTUV010000000
VERSION     DASTUV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859459"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(547..927)
                     /gene="gcvH"
                     /locus_tag="VFT03_07000"
     CDS             complement(547..927)
                     /gene="gcvH"
                     /locus_tag="VFT03_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU4847929.1"
                     /translation="MSVPEELQYTRSHEWVRTEGDTATIGITDHAQDELGDIVFVELP
                     ETGATFDAGDSFGTVESVKAVSDLYTPVGGEVVEVNEALSESPEKINEDPYGEGWIVK
                     IQVSGEADLLSASDYEQFLEEEES"
     gene            complement(942..2027)
                     /gene="gcvT"
                     /locus_tag="VFT03_07005"
     CDS             complement(942..2027)
                     /gene="gcvT"
                     /locus_tag="VFT03_07005"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU4847930.1"
                     /translation="MANEAPRRTPLYDEHKALGARLVDFAGWEMPVQYEGIKAEHNAV
                     RNHAGLFDVSHMGEAVFCGPDAEEAVQRLVTRDVSRLDEGQAGYSAVCYEDGGTVDDV
                     IVYRRSDDFLIVVNASNREKDLDHFKNNTDDLDVEVADESDDWALLALQGPEAADILQ
                     DLTDTDLSPLKAFRFVEGEVAGARAIISRTGYTGEDGFELYLSPDDAPRVWREILEAG
                     ATPAGLGARDTLRLEAGMCLYGNELDPETTPLEAGIGFAVHLDKEREFIGQEALKREK
                     EEGLRKKLVGFEVEGKGIARHDYPVKVEEDDVGRVTSGTLSPTLGKAIGLALVSPGVE
                     DRFEVVIRSRPVEAHTVPLPFYKREKK"
     gene            complement(2030..>2527)
                     /locus_tag="VFT03_07010"
     CDS             complement(2030..>2527)
                     /locus_tag="VFT03_07010"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566095.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEU4847931.1"
                     /translation="DDRYTFVRPEKSIGRMAAFHGNFGQVLRAWNYIKMHGPDLKDVT
                     DMAVLNANYVRARLRGTYRIPYDELCKHEVVVQPPKGKKALDIAKGLIDHGFHPPTIY
                     FPLVVKEAMLIEPTETESKETLDDFVGAMLELAESEELEEAPKEAPTRRLDEARAARQ
                     LKARW"
BASE COUNT          418 a          780 c          811 g          518 t
ORIGIN      
        1 ccttctcagc acgctcgggc ctttagaagg ccctcgcttc tcagcatttc agcttactct
       61 ctctgtactg agagaagctg acaagccctc gaagagggcg tgctgagagc gcggccctaa
      121 aagggccgaa gcgtgctgag aagcggaagc cgaaggctgg agcgtgctga caagccgacc
      181 gcagggaggc gtgctgaaaa agagaagaac ccgggacact cttaggggtg ataggtgggg
      241 aaagggaaag gaggagtgcg ccccgggctc tttaattgtg tcggtttagc atcgcaccaa
      301 ccgactgatt agaatgatac ctcgctcgtt acactttgta aaggtcttga aaccacgaat
      361 ttagaaactt tttgcaaata cctgcgaaat gcacatagtt agcgggcttt gcaccaagcg
      421 tccgaacaga agaagggctg gggttgctac gccctggtct gcgaatcggt tcaccggatg
      481 ctatttcacg cagcggcacg ggtaaggggt ggtaaggcgt cgtattcgcg gtaggcctta
      541 cgcgttctaa gactcttcct cttcgaggaa ctgctcgtag tctgaggcgg agaggagatc
      601 ggcctctccg gagacctgga tcttgacgat ccagccttcc ccgtaggggt cttcgttgat
      661 cttctcgggg ctttcgctaa gggcctcgtt gacctcgacg acttcgccgc cgacgggggt
      721 gtagaggtcc gagactgcct tgacggactc gacggtgccg aaggagtccc cggcgtcgaa
      781 ggtggctccc gtctcgggca gctcgacgaa gacgatgtcg ccgagctcgt cctgggcgtg
      841 gtcggtgatg cctatggtgg cggtgtcgcc ctcggtgcgg acccactcgt ggctcctggt
      901 gtactggagc tcctccggta cgctcaagct tcctcccctc gctacttctt ctcgcgcttg
      961 tagaacggca gcggcacggt gtgggcctcg acgggccggg accggatcac cacctcgaac
     1021 ctatcttcta ccccggggga gacgagcgca agccctatgg ccttgcccag cgtcggcgac
     1081 aacgtcccgc tggtcacgcg cccaacgtca tcttcctcca ctttcacggg gtagtcgtga
     1141 cgtgcgatgc ccttcccctc cacctcgaag cctacgagct tcttgcgcag cccctcctcc
     1201 ttctcccttt tcagagcctc ctggccgatg aactcccgct ccttgtccag gtggacggcg
     1261 aagccgatgc cggcctccag cggcgtcgtc tcgggatcga gctcgttgcc gtagaggcac
     1321 ataccggcct ccaaccttaa agtgtcgcgc gctcccagcc ctgccggggt cgcgccggcc
     1381 tcgaggatct cgcgccagac gcgtggggcg tcgtcggggg acaggtagag ctcgaagccg
     1441 tcttccccgg tgtatccggt gcgggagatg atggccctgg cgccagccac ctctccttcc
     1501 acgaaccgga aagctttcag gggagacagg tccgtatcgg tgaggtcctg caggatatcc
     1561 gcggcctcgg ggccttgcag cgccaggagc gcccagtcgt cggactcgtc tgcgacctcg
     1621 acgtcgaggt catccgtgtt gttcttgaag tggtcaaggt ccttctcgcg gttcgaggcg
     1681 ttcacgacga tgagaaagtc atcggagcgc cggtagacga tcacgtcgtc gacggtacca
     1741 ccgtcctcgt agcagaccgc ggagtagccg gcctgtccct cgtccagccg tgacacatcc
     1801 cgggtcacca gccgctggac ggcttcttcg gcgtcggggc cacagaagac ggcctcgccc
     1861 atgtgggaga cgtcgaagag gccggcgtgg ttacggaccg cgttgtgctc ggcctttatg
     1921 ccctcgtact gcacgggcat ctcccaaccc gcgaagtcca cgagccgggc gcccagagcc
     1981 ttgtgctcgt cgtagagcgg ggtgcgccgg ggggcttcgt tggccaccgt taccacctgg
     2041 ccttcagctg gcgggcggcg cgggcctcgt cgaggcggcg ggtgggtgcc tctttcgggg
     2101 cttcctcgag ctcctcgctc tcggcgagct cgagcatcgc gccgacgaag tcgtcgaggg
     2161 tctctttgga ctcggtctcg gttggctcaa tgagcatcgc ctccttgacg acgaggggga
     2221 agtagatcgt cggcgggtgg aagccgtggt ctatgagccc cttggcgatg tcgagcgcct
     2281 tcttcccctt gggcggttgt accacgacct catgcttgca cagctcgtcg taggggatgc
     2341 ggtaggtgcc gcggagcctg gccctcacgt agttggcgtt cagcacggcc atgtccgtca
     2401 cgtccttgag gtcgggtccg tgcatcttga tgtagttcca ggcgcgcaga acctggccga
     2461 agttaccgtg gaaggcagcc atccgcccga tactcttctc gggccggacg aaggtgtatc
     2521 tatcgtc
//
LOCUS       DASTUV010000349         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_897916, whole genome shotgun sequence.
ACCESSION   DASTUV010000349 DASTUV010000000
VERSION     DASTUV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897916"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFT03_07015"
     CDS             <1..562
                     /locus_tag="VFT03_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4847932.1"
                     /translation="ADPSGVRRELLNLKEDDDLPIVLVMGGGLGGGELENAVSFLFEA
                     SAAMHLVVLCGSNNRTRERLERRVAGEGHSATFLAFTDRVRELMAGATALVTKPGGMS
                     CTEALASALPQVLYNPIPGQEEDNAAAMVRYGAGVVVKGMGEILDETMEILTSPEHRR
                     QMVQCARAAHRPHSARSAAELILKGL"
     gene            562..1389
                     /locus_tag="VFT03_07020"
     CDS             562..1389
                     /locus_tag="VFT03_07020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4847933.1"
                     /translation="MKHPRARAWVAAAGISAGGAAIAGLRTATGRPLRSPPTLAAAGV
                     GLAVGGFMVYEWLSPRSWLYGRVFWHARTPERVAALTFDDGPCHPYTEQLLEVLNREG
                     VRATFFQIGNNVRREPNLAAEVASEHVVGNHTYDHPHLVWSRSGTVREELQRGQEAIH
                     DATGTLPDLFRVPHGWYGPQVISTAEELGFRCVGWSVMAWDWDRPPTDVIQRRILHGL
                     SPGGVSLLHDGQDTDAFPKADRSHTIAAVPDIIGSLKASGYRFLTLPELMDLDARTR"
     gene            complement(1346..>3020)
                     /locus_tag="VFT03_07025"
     CDS             complement(1346..>3020)
                     /locus_tag="VFT03_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU4847934.1"
                     /translation="PGGIFRAVELFSSGEVRMRSLGAAVDGSGYGRAHVDLYARRGVL
                     TKLRGRVRPLGFEAPGAGFEREVRLEAGTNRFDVGFHLPQVRRWWTWDRGEQPLYEVE
                     VSCGEEKETVRCGARTVELRDWRIYLNGERVFMRGINYLPTDVYPARASEERLRADAS
                     LVRGAGMNAVRVHVHVAERSFYEACDELGLLVFQDFPLQWTHKREVLEPAIRQAREMA
                     RDLRSYPSVGVYLAHDEPFYVVPPEKWNLPNLLRTGVDVLSPRWALWQRRVLGPAVVR
                     SIEEEDASRPVIDAAGHPLTTNHLYFGWYYGRFRYLERVVKIFPGLSRLPTEYGAQAL
                     PDPESLEEIWPSGTEPDWDALSLNYRLQPQRMLRYVPWKGDRALFARMSQDYQAEVLK
                     HATELFRRRKYHPTGGTFAFMLNDPAPAISWSVVDWRRRPKAAFEVLKTAMAPVLICA
                     EYPEESYEAGAALSLPLFVVNDLARDLGEVSWSWELLLGESTIAREEGEAEIAGDSVV
                     RIGEARAALPAPGDAVLVLRLSRKDASASNRYGFRISESGRRGPSARGG"
BASE COUNT          520 a         1068 c          939 g          493 t
ORIGIN      
        1 cgccgaccca agcggcgttc gtcgggagct actgaatctc aaggaggacg acgacctccc
       61 catagtgctc gtgatgggcg gggggctcgg cggaggagag ctggagaatg ccgtatcctt
      121 cctcttcgaa gcgagcgcgg ccatgcacct cgtcgtgctc tgcggctcga acaatcgtac
      181 ccgcgagcgg ctggagcgga gggttgccgg ggaagggcac tccgcgacct ttctcgcttt
      241 cacggaccgg gtgcgggaac tcatggcggg agccacggcg ctggtcacga agccgggcgg
      301 catgagctgc acggaggccc tcgcctccgc cctgccccag gtcctctaca atccgattcc
      361 cggccaggag gaagataacg ccgctgccat ggtacgctac ggcgctggcg tcgtcgtcaa
      421 agggatgggc gagatccttg atgagactat ggagatcctc accagcccgg agcaccggcg
      481 ccagatggta cagtgcgcca gggctgccca tcgcccgcat tcggccagat ccgccgccga
      541 gctgatcctg aagggccttt aatgaagcac ccgcgggctc gtgcctgggt agccgccgcc
      601 gggatctccg ccggcggcgc ggccatcgcc ggcctgcgaa ccgccactgg acgcccgctc
      661 cgctcgccgc cgaccctggc cgccgcggga gtgggattgg cagtcggcgg gttcatggtc
      721 tacgagtggc tctcgccccg gtcgtggctc tacggccgcg tcttctggca cgcccgcacg
      781 ccagagagag tggccgccct gaccttcgac gacgggccgt gccacccgta caccgaacaa
      841 cttctcgagg tcctgaaccg ggagggagtc agggctacgt tcttccagat cgggaacaac
      901 gtcaggcgcg agcctaacct ggcagccgag gtcgcctccg agcacgtagt cggcaaccac
      961 acctatgacc acccgcacct cgtctggagc cggtctggca cggtcaggga agagctgcag
     1021 cgcggtcagg aagccatcca tgacgctacc ggcacccttc cagacctctt ccgggtaccc
     1081 cacggatggt acgggccgca ggtcatctcg accgccgaag agctaggttt caggtgcgta
     1141 gggtggtcgg taatggcgtg ggactgggac cgtccaccaa ccgatgttat ccagcgccgg
     1201 atactgcacg gcctcagtcc cggcggcgtc agcttgctgc acgacggaca ggacaccgac
     1261 gccttcccga aggcggaccg ctcccacacc atagccgcag tccccgacat catcggcagc
     1321 ctcaaggcga gtggctaccg tttcctcacc ctccccgagc tgatggacct cgacgcccgg
     1381 actcgctgat acggaacccg taccggttgc tggccgaggc atccttcctc gatagacgca
     1441 gcacgagcac ggcgtccccc ggagcaggga gcgcagccct ggcttcccct atcctcacca
     1501 ccgaatctcc tgctatctcc gcctccccct cttcgcgcgc tatggtggac tctccgagga
     1561 gcagctccca gctccagctc acctcgccaa gatctcgtgc gaggtcgttc acgacgaaca
     1621 gcggcaggga gagcgcggcg cccgcctcgt agctctcctc gggatactcc gcgcagataa
     1681 gaaccggggc catggcggtc ttcaggacct cgaaagccgc cttcggcctg cgtctccagt
     1741 ccacaacaga ccacgaaatg gcgggtgccg ggtcgttgag catgaaagcg aacgtccctc
     1801 ccgtagggtg gtacttgcgc cgccggaaca gttccgtggc gtgcttgagc acctcggcct
     1861 ggtagtcctg gctcatccgc gcgaacagcg cccgatcccc cttccagggg acgtagcgca
     1921 gcatccgctg cggctggagg cggtagttaa gcgagagggc gtcccagtcg ggctccgtcc
     1981 cggagggcca gatctcttcc agcgactccg ggtcggggag cgcctgcgcc ccatattctg
     2041 tcggcagccg cgagaggcca gggaagattt tcacaacccg ctcgaggtac ctgaacctcc
     2101 cgtagtacca accgaagtac aggtggttcg tcgtcagggg gtggccagcc gcgtctatca
     2161 cggggcggga tgcatcctcc tcctcgattg agcgcaccac cgcgggaccc agcacccgcc
     2221 gctgccacaa tgcccagcgc ggcgagagca catcgacccc ggtgcgcagg aggttcggca
     2281 gattccactt ttcgggaggc accacgtaga agggctcatc gtgggccagg tagaccccga
     2341 cagaagggta gctgcgcaga tccctggcca tctctctcgc ctgcctgatg gcgggttcca
     2401 ggacctccct tttgtgcgtc cattgcaggg gaaagtcctg gaagacgagg agcccgagct
     2461 cgtcgcaggc ttcgtagaag ctcctctccg ccacgtggac gtgaaccctg acggcgttca
     2521 tgccggcccc gcgcaccaga gaggcatccg cgcgcagccg ctcttccgag gccctggcag
     2581 ggtagacgtc cgtgggcagg taattgatcc cccgcatgaa gacgcgctcg ccgttgagat
     2641 agatccgcca gtcgcgcaac tccacggtac gcgcgccgca gcgcactgtc tccttttcct
     2701 caccgcagct tacttccacc tcgtacagcg gctgctctcc cctgtcccag gtccaccagc
     2761 gacggacctg cggcaggtgg aaacccacgt cgaagcggtt ggttcccgct tccaaccgca
     2821 cctcgcgctc gaacccggcg cccggggctt cgaatcccag gggccgcacc cttcccctga
     2881 gcttcgtcag tactccccgg cgggcgtaca ggtcgacgtg cgccctaccg tatcccgagc
     2941 catccacggc ggctcccaga gatctcatgc gtacctcacc actcgagaag agctccaccg
     3001 ccctgaagat gccgcccggg
//
LOCUS       DASTUV010000350         3609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1076172, whole genome shotgun sequence.
ACCESSION   DASTUV010000350 DASTUV010000000
VERSION     DASTUV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076172"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFT03_07030"
     CDS             <1..1260
                     /locus_tag="VFT03_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4847935.1"
                     /translation="IDTCDFFTGEGRRLYGQTVPSEMPDKQLFTFRMPVGVAAIITAG
                     NFPVAVPSWYMVPALTCGNAVVWKPAEYSPAIGDALAQLFIHAGLPDGVLNVVQAEGP
                     AAFEGLERALDEGLVDKVGFTGSSAVGQKIGELCGHHMQSPCLELGGKNPLVVMPDAN
                     LDLAVEGALFSGFGTAGQRCTSLGTAIVHESVHDEFLTRFSRAAEEAKIGDPTHDVLY
                     GPMLSQRFHDRFLGWLELIESHHAISGSTGMGTITRDNPRSGFVGDPEAGIYCHPTIV
                     DGVTMDDELYRTETFGPIVGVAEFSTFDEAVALSNNHGYGLSAAIYTRDAENALRFRE
                     RNTAGMLSVNNSTSGAEAHLPFGGNGKSGNGSRLSGIWVLDQFTRWQAMNWDYAGKLQ
                     KAQMDIVELPADLDYTLPGAESERDGV"
     gene            1257..2408
                     /gene="solA"
                     /locus_tag="VFT03_07035"
     CDS             1257..2408
                     /gene="solA"
                     /locus_tag="VFT03_07035"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEU4847936.1"
                     /translation="MTHDVIVAGLGGMGSAAAYQLAGRGQRVLGLERFSPAHDRGSSH
                     GRSRIIRQAYFEGSEYVPLLLRAYELWEQLEEETGQRLMTLTGGLMIGREDGELVSGS
                     VRSAEEHGLPYEMLDAAEIRRSFPAYAPGPETVALYEEKAGFVRPEETVKAHLDQASS
                     SGADLRFGEPVLSWEASGDGVRVETPKSTYEAGRLVISPGAWAPQVLADLDLPLEVIR
                     QVMFWYEPKNGLGPFLPERFPIFIWEPEDGNMFYGFPAQDADRGVKAAFFRAGGVPTS
                     PDTIDREVHEEEIGFLRGYLAEHVPELAGRCLDARACMYTNTPDEHFVISAHPEHPQV
                     VIACGFSGHGYKFCSVVGEIVADLAIEGSTRHPIDLFSPARLNRADARG"
     gene            2411..3322
                     /locus_tag="VFT03_07040"
     CDS             2411..3322
                     /locus_tag="VFT03_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HEU4847937.1"
                     /translation="MEILSAEETGTRLPYADLAESIREVALERTSGAVHAPSRMALPL
                     EGGGVLLVMPASGEGIAMTKLVTVHPENARSGLPTIQGEVVVMEAGTGMRLGILDGSV
                     VTARRTAALSLLAARELALRPNGPLLIVGSGTQGRAHLEAFHEGLGTSRVFVNSRTEE
                     SARSLAEHAENLGMEARVVGEPGEALGEASLVVTATTSREPVLPDGVSDGSFVAAVGS
                     FEPGAAELPAGLVARATVVVDTLEGAKEEAGDLIQAEKAGAFSWENVTQLEDVLRNPE
                     RPEGTVVFKSVGHALWDLAAARTVFGG"
BASE COUNT          614 a         1219 c         1208 g          568 t
ORIGIN      
        1 atagacacct gcgacttctt caccggcgag ggacgccgcc tctacggcca gaccgtgccc
       61 tcggagatgc cagacaagca gctcttcacg ttcaggatgc cggtcggtgt cgcggcgatc
      121 atcaccgcgg gcaacttccc cgtcgccgtc ccttcctggt acatggtccc cgccctgacc
      181 tgcggcaacg ctgtggtctg gaagccggca gagtattcgc cggcgatcgg cgacgccctc
      241 gcccagctct ttatccacgc cggcctgccc gacggcgtcc tgaacgtcgt ccaggccgag
      301 ggaccggccg cattcgaggg cctggagagg gcgctcgacg aggggctcgt ggacaaggtc
      361 ggcttcacgg gctcatctgc ggtcggccag aagataggcg agctctgcgg gcatcacatg
      421 cagtcgccgt gcctcgagct cggcggcaag aacccgctcg tcgtcatgcc cgacgccaac
      481 ctcgacctcg ccgtcgaggg cgcgctcttc tccggcttcg gcacagccgg ccagcggtgt
      541 acctcgctcg gcaccgcgat agtccacgag tcggtgcacg acgagttcct cacccgcttc
      601 tcccgggccg ccgaagaggc gaagataggc gacccgacgc atgacgtcct ctacggaccg
      661 atgctgagcc agaggttcca cgaccgcttc ctcggctggc tcgagctcat agagagtcac
      721 cacgccatca gcggctcgac cgggatgggg accataacca gggacaaccc gagatcaggc
      781 ttcgtcgggg atccggaggc cggcatctac tgccacccga ccatcgtgga cggcgtcacc
      841 atggacgacg aactttacag gaccgagacc ttcggtccca tcgtcggcgt cgccgaattc
      901 tcgactttcg acgaggcggt cgccctctcg aacaaccacg gctacgggct ctcggcggcg
      961 atctacacgc gcgacgccga gaacgcgctg cgcttccgcg agaggaacac cgcagggatg
     1021 ctctccgtca acaactcaac cagcggggca gaggcgcacc tgcccttcgg cggcaacggc
     1081 aagagcggca acggcagccg gctctcgggg atctgggtcc tcgaccagtt cacccgctgg
     1141 caggcgatga actgggacta cgcgggcaag ctccagaagg cccagatgga catagtcgag
     1201 ctgcccgccg acctcgacta cacgctgccc ggggcggagt cagagaggga cggggtttga
     1261 cccacgacgt catcgtcgcc ggcctgggcg ggatgggcag cgccgccgcc taccagctcg
     1321 caggccgggg tcagcgtgtc ctcgggctgg agaggttctc cccggcccat gacagaggct
     1381 ccagccacgg ccgctcgcgc atcatccgcc aggcctactt cgaagggtcc gagtacgtcc
     1441 cgctcctgct gcgcgcctac gagctctggg agcaactgga ggaggagaca gggcagcggc
     1501 tcatgacgct cacgggcggg ctgatgatcg ggcgcgagga cggtgagctc gtctccggca
     1561 gcgtccggag cgccgaagag cacggcctgc cctacgagat gctcgacgcc gccgagatcc
     1621 ggcgcagctt cccggcctac gcccctggcc ccgaaaccgt cgccctctac gaggagaagg
     1681 ccggcttcgt ccgccccgaa gagaccgtaa aggcccacct cgaccaggcg agctcttcag
     1741 gcgccgacct gcgtttcggg gagccggttc tctcgtggga ggcgtcgggg gatggagttc
     1801 gggtcgagac gccgaaaagc acctacgagg cggggagact cgtcatctca ccgggcgcct
     1861 gggcccctca agtgctcgcg gacctggacc tcccgctcga ggtgatccgg caggtgatgt
     1921 tctggtacga gccgaagaac ggtctggggc ccttcctgcc tgaacgcttc ccgatcttta
     1981 tctgggagcc tgaagacggc aacatgttct acggcttccc ggcccaggac gctgataggg
     2041 gtgtcaaagc cgccttcttc cgggccggcg gcgtccccac cagccccgac acgatagacc
     2101 gcgaggtgca cgaagaggag atcggcttcc tccgcggtta cctggcagag cacgtccccg
     2161 agctcgcggg acgctgcctc gacgcgcggg cgtgcatgta cacgaacacc ccggacgagc
     2221 acttcgtgat ctccgctcac cccgagcatc cgcaggtagt catagcctgc gggttctcgg
     2281 ggcacggcta caagttctgc agcgtggtgg gcgagatcgt cgctgacctg gctatcgaag
     2341 gctccacccg ccacccgata gatctcttct cccctgcccg cctgaacaga gccgacgcgc
     2401 ggggctagcg gtggagatcc tgagcgcaga agagaccgga acacgtttgc cgtacgcgga
     2461 tctcgccgag tccatccggg aggtcgcgct ggagcgcacg tccggcgcgg tgcacgctcc
     2521 aagccggatg gcgctgcccc tggagggggg tggcgtcctc ctagtcatgc ccgcctcagg
     2581 cgagggaata gccatgacca agctggttac tgtccacccg gagaacgcgc gcagcggcct
     2641 gccgacaata cagggcgaag tcgtggtaat ggaggccggg acgggtatgc ggctggggat
     2701 actggacggc agcgtcgtca ccgccaggcg caccgccgcg ctctcgctgc tcgccgcccg
     2761 cgagctcgcc ctcagaccca acggtccgct cctgatcgtg ggctccggta cccagggccg
     2821 cgcccacctg gaagcgttcc acgagggcct cggcacgtcg agggtattcg tcaactcccg
     2881 caccgaggag agcgccaggt ctctggccga gcacgcggag aaccttggca tggaggcgcg
     2941 ggtggtcggg gaacccggag aggcactcgg tgaggcgagc ctcgtggtca cggccacgac
     3001 tagccgtgag ccggtgctcc ctgacggcgt atccgacggc tccttcgtag ctgcagtggg
     3061 ctcattcgag cccggagcgg cggagcttcc ggctgggctc gtggcgcgcg cgacagtcgt
     3121 ggtcgacact ctagagggtg cgaaggagga ggcaggggat ctgatccagg ccgagaaggc
     3181 cggcgctttc tcctgggaga acgtcaccca actggaagac gtcctccgta atccggaacg
     3241 ccccgaaggg accgtcgtct tcaagagcgt ggggcacgcg ctctgggacc tggccgccgc
     3301 ccggacggtc ttcggcggtt aaaaggggcg aggagctacc gaaagccttc ctcagctcgc
     3361 cttttgctcg tcgcggtcgg gctctgtgtc gccgcatcct tccgacactc cagacgtata
     3421 attggtggac ctggatctcc actaggagga aggatggtcg caaggttcct tctcacggcg
     3481 ctgatgctcg cggtatcggc aagttttcta gcgccgttcg cggcggcgca gggcccggac
     3541 acggtcttcg acgaggcgaa cgtgctcagc gccgccgagg agcgggacgt tcaggcggcc
     3601 tttgatgag
//
LOCUS       DASTUV010000351         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_779916, whole genome shotgun sequence.
ACCESSION   DASTUV010000351 DASTUV010000000
VERSION     DASTUV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779916"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1105)
                     /locus_tag="VFT03_07045"
     CDS             complement(179..1105)
                     /locus_tag="VFT03_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEU4847938.1"
                     /translation="MTLSVGCGGGGGETTATPPATPAASTPAATGTGTTVGIGQAETL
                     KDIPFSLNTDHPVPPDFREAYERRALISVQFYKEGTDPLGYPEGLDVDRKVRATMDRL
                     RTRYPTIEFFNYDIDNPGTADKSEGLERGQYGTLAAQLGVGFTPFVAMLAPSGEQYVI
                     TNLYQGYVSPPVLSQALYDLSSVKVEDNTSDVDVVLEQLDLTESGGGLEYVTIHNRST
                     RPVNLQGFSLRVLDPESAEVDPDAPGVTITDPIEVQPGQSVSIGRVPSVVDANGEKVA
                     GTFEGGESLELSPGDQVALLDSGGAVASTITV"
     gene            1343..>2034
                     /locus_tag="VFT03_07050"
     CDS             1343..>2034
                     /locus_tag="VFT03_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU4847939.1"
                     /translation="MPKEGRNTVNPYRTHNAGQLRAGDVGERVRLAGWVNRRRDHGGL
                     IFIDLRDRWGITQVTFDPEREEVFAAAERLRPEWSVSVEGEVVRRPEGNENPDLPTGE
                     IEVEAAALDVLNESETPPFEIDRETPVDELLRLRYRYLDLRRSRMRDNILFRHEVIKR
                     MRDYLDERDFVEVETPLLTRSTPEGARDYLVPSRLYSGQFYALPQSPQQFKQLLMVAG
                     FERYFQIARALR"
BASE COUNT          373 a          652 c          665 g          344 t
ORIGIN      
        1 tattgccgca aagccgtctc gtaatgcgtg accgggcaag tgcccaacgg cggagaaagc
       61 tgacaagcga aggcgaagcc tgagcgtgct gagaaggcgt ctggcgaagc caggcgccgt
      121 gctgacaagc cgaccgtagg gaggcgtgct gacaagccga ccgcagggag gcgtgctact
      181 acaccgttat cgtggaggct acggcgccgc ccgagtcgag cagggctacc tggtccccgg
      241 gtgagagctc cagcgactcg ccgccctcga aggttccggc caccttttca ccgtttgcgt
      301 cgacgacgct ggggacacgg cctatggaga cgctctggcc gggttggacc tcgataggat
      361 ctgtgatcgt cactcccgga gcgtcgggat ccacctccgc ggactccggg tccaggaccc
      421 tcaaagagaa gccctggagg ttaacgggtc tggtgctgcg gttgtggatg gtcacgtact
      481 cgaggccgcc gccgctctcg gtcaggtcga gctgctccag taccacgtcg acgtcgctcg
      541 tgttgtcctc gaccttgacg gaagagaggt cgtagagcgc ctggctcaat accggcggcg
      601 agacgtaacc ctggtagagg ttcgtgatca cgtactgctc gccgctcggc gccagcatcg
      661 cgacgaaggg cgtgaagccg accccgagct gcgccgctag cgtgccgtac tggccgcgct
      721 cgagcccctc gctcttatcg gccgtccccg ggttgtcaat gtcgtagttg aagaactcga
      781 tcgtcgggta tcgggtccgg agcctgtcca tggtagcgcg caccttccgg tccacatcaa
      841 gcccctccgg atagccgagg gggtccgtcc cctccttgta gaactgcacg gagatcaggg
      901 ctcgtcgctc gtacgcctcc ctgaagtcag gcgggacggg atggtcggtg ttgagcgaga
      961 aaggtatgtc tttgagggtc tccgcctgcc cgatcccgac ggtcgtcccg gtccccgtgg
     1021 ctgccggggt gctggccgcg ggagtcgccg gtggcgtcgc ggtcgtctcg ccgcccccgc
     1081 ccccgcaccc aaccgagagg gtcaagatca atccaataaa cagcccgatt ctggcaggca
     1141 aactcctgac cccgtctact cctataacca tctcccctgc ggcttcccgg ttacccgcga
     1201 gagccgtgct cgagttagag gctaacttag cacgcgcgca aaaagagcgc aacgcgccat
     1261 ctcggacggg atgggctaac atttgcaaac cgcgcgcccg aagacgaacg ggtgcaaggg
     1321 cggcagagag gagagattac ccttgcctaa agaaggcagg aacaccgtca acccatatag
     1381 aacgcacaac gccggccagt tgcgcgcagg tgacgtcggc gagcgcgtta ggctggctgg
     1441 ctgggttaac cgcaggaggg atcatggagg tttgatcttt atcgacctgc gcgaccgctg
     1501 ggggatcacg caggtcacct tcgaccccga gcgcgaggag gtcttcgccg cggctgaacg
     1561 gctcaggcca gagtggtcgg tctcggtcga gggagaggtc gtccggcgcc cggagggcaa
     1621 cgagaaccct gacctgccga caggcgagat cgaggtcgag gcggccgcac tcgatgtgct
     1681 gaacgagtcc gagacgccgc cgttcgagat cgaccgcgag acgcccgtgg acgagcttct
     1741 gcgcctgaga taccgctacc tcgacctcag gcgctcccgg atgcgggaca acatcctctt
     1801 caggcacgag gtcatcaagc gtatgcgcga ctacctcgac gagcgggact tcgtcgaggt
     1861 agagacgccg ctgctgacca ggtcaacccc cgaaggggcc cgcgactacc tggtgccgag
     1921 caggctctac tccggccagt tctacgccct cccgcagtct cctcaacagt tcaagcagct
     1981 gctcatggtc gcgggcttcg agcgctactt ccagatagcg cgcgcgctgc gcga
//
LOCUS       DASTUV010000352         5384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1432483, whole genome shotgun sequence.
ACCESSION   DASTUV010000352 DASTUV010000000
VERSION     DASTUV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432483"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VFT03_07055"
     CDS             complement(<1..126)
                     /locus_tag="VFT03_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU4847940.1"
                     /translation="MARVVGVPKEIKDQEGRVSMQPDGVAELVHHGHEVVVEAGAG"
     gene            288..1097
                     /locus_tag="VFT03_07060"
     CDS             288..1097
                     /locus_tag="VFT03_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4847941.1"
                     /translation="MALGRGSVGFSIGEVSQAIGLAPQTLRLWERERLVRPRRTDRGY
                     RVYTEPDVERLRRIKQLRKVEGLNFAAIRKQLGTADEGTRDNGFAGSPNAMGERLRRL
                     RTRAHKTLKEVSQATGLSISFISALERGGSGASIASLRRLAEAYGVTMRKLFGADLDQ
                     SFPLVRSEDRPVMQWDNGVRFEEMASGEKVMDPSYIRVPAGAGSEGFYSHNGEEFVYV
                     ISGVLFVELKDQGTYRVAPGDTLYFPSTTQHRWWAADEPVEAVYVNTPPTF"
     gene            1201..3033
                     /locus_tag="VFT03_07065"
     CDS             1201..3033
                     /locus_tag="VFT03_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU4847942.1"
                     /translation="MNPDSRLEALLSVPVLHGPQVSPDGRWVAWSWSRLGPAADVFAA
                     PTDGSALPIRLTETADDTLVVSWTPDSRAVVVGQDWDGNERTRLFRVGLEEPGVMEPL
                     TEQSPGYYVWGGQMHGRWLVYGANVDVETGEEIEETWIYRQDLRSGEREVLARPRRGN
                     FYAPQLNTPGDQILYHRNDIDPAGLQVWLVDVEGRDDREILNFGPRVKVYASWFPDGR
                     RVLFVAETETHRRLGVWDEGTVRWLLDDPERNLEYAFVPPNGGPVVAVEVVRSQVQAT
                     LLNPVTGTEERPYLAAGNAIPLSPTGGESWVGLHYDARHPVDLVLYDPTEATKTPASL
                     TGFWERVPLEPGELAAAEDFTWTSVDGLEVQGWLYRTPGRSTGTIVLIHGGPTHHAED
                     RFNAQVQYLVSRGFDVLTPNYRGSTGFGLWFQETIKEDGWGGREQEDIRYGIEALVRA
                     GVAEPGRAGVTGTSYGGYSAWWAITHYPTDLVAAAAPICGMTDLVVDYHATRPDLRPY
                     SEEMMGGSPEEVPDRYRARSPVNFVENIRGELLIVQGLRDPNVPPDNVHAVTKALGKH
                     NIPYELLTFENEGHGIARPENLKVLYPRLADFFERAFSKPSRGQ"
     gene            complement(3103..3549)
                     /locus_tag="VFT03_07070"
     CDS             complement(3103..3549)
                     /locus_tag="VFT03_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847943.1"
                     /translation="MGRMAMVLTAVALMVVLSAGVAVAASITGTEDRDVLDGSGKNET
                     ISGLGGPDTLNGAGGRDTIKGGDGGDEAGGGLGADEVYGGPGGDYLIDAPDNYRDVLH
                     ANSGEDNVQVRDFPAVKDVVYCGSGRDTAYVDRLDVVHDCEVVRLP"
     gene            3825..5180
                     /locus_tag="VFT03_07075"
     CDS             3825..5180
                     /locus_tag="VFT03_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU4847944.1"
                     /translation="MRGLGEGEDRTPLAGVENLLLHFTPYAEDWSKLPVIVSGEGCYV
                     TDDRGNRYIDGLAGLFTTQVGHGRTELGEVAARQMKELGFFPNWSFQHPRSLELARKI
                     SEIAPGDLDSTFFVSSGSEAVETVIKLARQYHKANGEATRYKVISRKIAYHGTTMGAL
                     SVTGLPTFRAPFEPLLQGFMHVPNTQQDPEGAADAIEEAIEFGPPETVAAVILEPVQN
                     AGGCLVPPPDYWRRVREICDRHGVLLVSDAVICAFGRLGEWFGIERFDVVPDMTSFAK
                     GVTSGYLPMGGVVVNEKMSETLKENAPMFLHGSTFGGHPVSAAVALENIRIIERERLL
                     ENVRNLEGYFEGELRRLAEQHPIVKEIRGMGFFWAVEVNAERADGTPLEGEEYQRYFK
                     GVVSRKLLEGGLICRFDDKDEPVIQFSPALVADREVIGRIAEVTDAALAELERELGYR
                     S"
BASE COUNT          956 a         1692 c         1817 g          919 t
ORIGIN      
        1 accggcaccc gcctccacaa ccacctcgtg tccgtggtgg accagctccg ccaccccgtc
       61 cggttgcatg gaaacgcggc cttcctggtc tttgatctcc ttcggaacac ccacgactcg
      121 agccatgctc tctcctcctc gcttctgcct cccccgtacc ttatatcagg ttactataaa
      181 cttgcaacga tactgtcaac ggcttttgcg caggcgctgc cgttgcagca actttgcaag
      241 tatactgtga ggctgatata tgggacgcta acgagacggg aggactgttg gcgcttggca
      301 gaggttcggt cgggttctcc ataggcgagg tttcgcaggc gatagggttg gcgcctcaga
      361 ctttgcggtt atgggagcgg gagcgtctgg tgaggccgcg ccgcacggac aggggctaca
      421 gggtttacac ggagccggat gtagagcggc tgcgtaggat caagcaactc aggaaggtcg
      481 aggggctgaa cttcgccgcc atacgcaagc agcttggcac cgccgacgaa ggcactcggg
      541 acaacggctt cgccgggtct ccgaacgcga tgggagagcg gctgcgacgg ctgcgcacgc
      601 gggcgcacaa gacgctcaag gaggtatcgc aggccacggg gctctccatc tcgttcatct
      661 cggcgctcga gcgcgggggg tcgggagctt cgatcgcctc gctgcggcgg ctggcggagg
      721 cgtatggggt gaccatgcgc aagcttttcg gggcggacct ggaccagagt ttcccgctgg
      781 tgcggagtga ggatcgcccg gtcatgcagt gggacaacgg cgtgcgtttc gaggagatgg
      841 cctcggggga gaaggtaatg gacccttcct acataagggt accggctggc gcggggagcg
      901 agggcttcta ctcgcacaac ggtgaggagt tcgtctacgt aatctccggc gtactgttcg
      961 tggagctcaa ggaccagggc acctatcggg tagcgccagg cgacacgctc tacttccctt
     1021 cgaccaccca gcaccgatgg tgggccgcag acgagcccgt cgaggcggtc tacgtcaaca
     1081 ccccgccgac cttctaaggt ggtttcaggc ttcagggttt cgtctcctgg accgatccta
     1141 tcggcgggcg cggagcccac ggttcccggt cgatcgagaa aaaggtagta gtcctcattg
     1201 gtgaaccccg atagtcggct cgaggctctg ctctccgttc ctgtgctgca cggtccgcag
     1261 gtctccccgg acggccgctg ggtcgcatgg agctggtcgc ggctcggtcc tgcggcggac
     1321 gtgttcgcgg cgcccaccga cggctcggcg cttcccattc gcctaacgga gacagcggac
     1381 gacacgctgg tcgtgtcctg gaccccggac agccgcgccg tcgtggtggg ccaggactgg
     1441 gacgggaacg agcgcacgcg gctcttcagg gtcgggctcg aggagccggg cgttatggag
     1501 ccgctcaccg aacaatcacc cggctactac gtctggggcg ggcagatgca cgggcggtgg
     1561 ctcgtctacg gggcgaacgt ggacgtagag accggggagg agatagagga gacctggatc
     1621 taccgccaag acctccgaag cggcgagcgc gaagtactgg cccgtccgcg gaggggcaac
     1681 ttctacgcgc cccagctcaa cacgccgggt gaccaaattc tctaccaccg caacgacatc
     1741 gacccggcgg gattgcaagt ctggctcgtg gacgtcgaag gccgcgacga ccgcgagatc
     1801 ctcaacttcg gtccccgggt caaggtctat gcctcgtggt tcccggatgg gcggcgggtc
     1861 ctcttcgtgg cggagacgga aacacaccgc aggctgggcg tgtgggacga aggaaccgta
     1921 cgctggctgc tggacgatcc ggagcgcaac ttagagtacg ctttcgtacc ccccaacggc
     1981 ggtcccgtcg tcgcggtgga ggtagtgcgg tcgcaggttc aggcgacgct gctcaaccct
     2041 gtaaccggga cagaagaacg cccgtacttg gccgcgggta atgcgatccc gctctccccg
     2101 accgggggag agagctgggt cgggctgcac tacgacgcca ggcaccccgt agacctcgta
     2161 ctctacgatc ccacagaggc taccaagacc ccggccagtc tcaccggatt ctgggagcgc
     2221 gtcccgctgg agccgggaga gctggccgcg gccgaggact tcacgtggac atccgttgac
     2281 gggctcgagg tgcagggatg gctgtaccgg acaccgggcc ggagcacggg gaccatcgtg
     2341 ctgatccacg gcggtcccac ccaccatgcc gaggaccggt tcaacgccca ggtccagtac
     2401 ctggtctcgc gcggcttcga cgtgctcact cccaactaca gggggagcac cgggttcggg
     2461 ctctggttcc aggagacaat aaaggaggac ggctggggcg gccgcgagca ggaggacatc
     2521 aggtacggca tcgaggcgct cgtccgggcc ggtgtggcgg agccgggtcg cgccggcgtc
     2581 accgggacct cgtacggcgg gtactccgcg tggtgggcga tcacgcacta tccgaccgac
     2641 ctcgtcgccg ccgcggcccc gatctgcggc atgacggacc tcgttgtgga ctaccacgcc
     2701 acccgccccg acctccgccc gtacagcgag gagatgatgg gcggctcccc cgaggaggtc
     2761 ccggaccgct accgcgcgcg ctcgccggtg aactttgtgg agaacatccg cggggagttg
     2821 ctgatcgtac agggtctccg ggacccgaac gtccccccgg ataacgtcca cgccgtcacg
     2881 aaagccctcg gcaaacacaa cattccctac gagctgctca ccttcgaaaa cgaggggcac
     2941 ggcatcgcgc gccccgagaa cctgaaggtc ctctacccgc gcctcgcgga cttcttcgaa
     3001 agggcgttct ccaagccctc ccgaggccag tagcgaaggg tcctcacaaa acgagggggc
     3061 gcggataaat ccgcgccccc gcccatccag ttctgctgtt acttacggca gccgcacgac
     3121 ctcgcagtcg tgaaccacgt ccagcctgtc cacgtaggcc gtgtcgcggc ctgacccgca
     3181 gtagacgacg tccttgactg cggggaagtc ccgtacctga acgttgtcct cgccggaatt
     3241 ggcgtggagc acgtccctgt agttgtcggg ggcgtcgatc aggtagtcgc cgcccggccc
     3301 accatacacc tcgtcggccc cgagcccgcc gcccgcttcg tcgccgccgt cgccgccctt
     3361 gattgtgtcc ctgcccccgg cgccgttgag cgtgtcaggg cctccgaggc cggagatagt
     3421 ctcgttcttg ccgctgccgt cgagcacgtc acggtcctct gtgccggtta tggaggcagc
     3481 cacggccacg cccgcggaga gaactaccat cagagccacc gccgtcaaaa ccatcgccat
     3541 ccgccccatg cccgtctcct cttcctcgcg cccagcctcc ccgagcgtgt acaaacactg
     3601 taagggacgc ggattacagg acggcaacgc ccggataacg cttcggagac actctcataa
     3661 cgcttcggta acggtcgaga gcccgctcgg atcctgcgaa caggagattg tcaggtttct
     3721 atattaccgt agcccgtacg tgtcttgacc cccttacagt aactgtgtag cattcatgca
     3781 aagaatctgg ggggttggtt cgaggaggtt acgggttgga tgtcatgcga ggtcttgggg
     3841 agggtgagga cagaacgccg ctcgctggcg tggagaacct cttgctccat ttcacgcctt
     3901 atgccgagga ctggtcgaag ctaccggtca tcgtctcggg tgaaggttgc tacgtcaccg
     3961 acgatagggg caaccgctac atagacgggc tagcggggct cttcacgacg caggtcgggc
     4021 acggcaggac ggagttgggc gaggtcgcgg ccaggcagat gaaggagctc gggttcttcc
     4081 ccaactggag cttccagcac ccgaggagcc tggagcttgc gcgcaagatc tccgagatag
     4141 cgccggggga tctcgactcg accttcttcg tctcctcggg ctccgaggcc gtcgagacgg
     4201 tcataaagct ggcgcggcag taccacaagg cgaacggcga ggcgacgcgc tacaaggtca
     4261 tctcgcgcaa gatcgcctac cacggcacca cgatgggcgc gctctccgtg acggggttac
     4321 ccactttcag ggccccgttc gagccgctgt tgcagggctt catgcacgtt ccgaacacgc
     4381 agcaggaccc ggagggggcg gctgacgcca tcgaggaggc catcgagttc gggccgccgg
     4441 agacggtagc ggcggtgata ttggagcccg tgcagaacgc gggagggtgc ctcgttccgc
     4501 cgcctgatta ctggaggcgg gtgcgagaga tctgcgacag gcacggcgtg ttgctcgtct
     4561 cggacgcggt gatctgcgcg ttcggacgcc tcggcgagtg gttcggcatc gagcgcttcg
     4621 atgtggtgcc cgacatgacg agcttcgcca agggagtcac gagcgggtat ttgccgatgg
     4681 gcggcgtcgt ggtgaacgag aagatgagcg agacgctcaa agagaacgcg cctatgtttc
     4741 tgcacggctc gaccttcggc gggcacccgg tctcggccgc ggtcgccctg gagaacatcc
     4801 gcatcatcga gcgcgagagg ctcctcgaga acgtccgcaa cctggaaggg tacttcgagg
     4861 gcgagctgag gcggctggcc gagcaacatc ctatcgtcaa ggagatccgg ggcatgggct
     4921 tcttctgggc ggtcgaggtc aacgcggagc gggccgacgg gacgcctctg gagggtgagg
     4981 agtaccagag gtacttcaag ggcgtcgtct cgcgcaagct cctggagggc ggcctcatct
     5041 gccgcttcga cgacaaggat gagccagtga tccagttctc ccccgcactc gtagccgaca
     5101 gggaggtcat aggcaggatc gcggaggtca ccgacgccgc tctggccgag ctggagcgcg
     5161 agctcggcta ccggagctag gatgagaacc gagtgcgcca gcgcatcagc aggagacagg
     5221 ggttggccgt cagcgtagag gagaagagcg ctcgctccag ggactacgct cgttacagct
     5281 tctggctgga gacggcaggc gaagagctcg tcccgaggcc gtcgctggag ggtccgatgg
     5341 aagccgacgt ggcggtcctc ggcgccggtt tcacggggct ctgg
//
LOCUS       DASTUV010000353         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1291605, whole genome shotgun sequence.
ACCESSION   DASTUV010000353 DASTUV010000000
VERSION     DASTUV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291605"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..981)
                     /locus_tag="VFT03_07080"
     CDS             complement(25..981)
                     /locus_tag="VFT03_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU4847945.1"
                     /translation="MKAATHLVFAGCCWFATSAVLDVRYETPAVLAAAATSLLPDADH
                     PGSWLGYQLGSISDDFNRLFGHGSFLHSLLALALATILLGFPPWWTLGSSSPMLAVFV
                     GYGSHLLAEMMTLDGVQLFWPSRLIAVFPGRDEYRVVSGGTSERIFVALALVFALLFY
                     PVSRVGFDGLIFRLGGDDQLYGRVAKVTDGDTITVEIGGRSTPVRLIGVDTPETVAPG
                     QPIGCFGQRASEYTKQILTKRFVRLRIPRIGDSEDAYGRTLAYVYLDTNGDGKYEHLF
                     NKDLLELGLARTTTFSHADRREFERLREDAEERGAGLWDSCP"
     gene            complement(978..1589)
                     /locus_tag="VFT03_07085"
     CDS             complement(978..1589)
                     /locus_tag="VFT03_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847946.1"
                     /translation="MDFDTSSGKGRGERRPPPGGGRPGLGRDLSVSGHRIRDGPEGEE
                     TGGFRIPAGSRKAALVHLALAAWRAGEFESRQVGEVRGREVDEAGLRRALYRSSMGEI
                     EHILGPLLKGRRVVVEGRLAGTGRTALERLGAEVEELRLQDERRYIDMTPAIFVFAAL
                     AMAVRYISLGLDDQDLYILAGIFFALMYGLRPFFFKMQRPRDE"
     gene            1674..1895
                     /locus_tag="VFT03_07090"
     CDS             1674..1895
                     /locus_tag="VFT03_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847947.1"
                     /translation="MAFPADRFEVGVAPVEKHPHEAVLVRQDAPPHASLHGSGKLGEA
                     FQSDAVVERTTARCVYDPYSKSQSPELQV"
     gene            complement(1956..>2484)
                     /locus_tag="VFT03_07095"
     CDS             complement(1956..>2484)
                     /locus_tag="VFT03_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847948.1"
                     /translation="LQRRLVFALALATGSELLLLDEPSAGVDPFARREMLEDISRFMN
                     DGVKTVVLATHVMEEVRRIADYVVLLADGDFLGLYEKDALLEQWKTFWVEREPGEDVP
                     GVVEVEGGSPVRLVSDSASVTAEALSARNVSILRSGALDLEEILSHLVRQSKEGRFAA
                     SSIESRHEGRPGRGG"
BASE COUNT          448 a          812 c          820 g          404 t
ORIGIN      
        1 gagcgtgcta acaagcgaag cgctctaggg acaggagtcc caaagacccg cgccccgctc
       61 ctcggcatcc tctcgcaacc gctcgaactc gcggcggtcg gcgtgggaga aggtagtcgt
      121 gcgagcgagg ccaagctcga ggaggtcctt gttgaagagg tgttcgtact ttccgtcgcc
      181 gttggtgtcg aggtagacgt atgcaagagt acgtccatag gcgtcttcgg agtcgccgat
      241 gcgaggaatc cggagcctca cgaagcgctt cgtgaggatc tgcttcgtat actccgatgc
      301 ccgctgcccg aagcagccga tgggctggcc gggggctacg gtctcggggg tgtcaacgcc
      361 gatcagcctg accggagtgc tccttccccc tatctctacc gtgatggtgt cgccgtccgt
      421 taccttcgcc accctgccat agagctggtc gtctccgccg aggcggaaga tcagaccgtc
      481 gaagccgaca cgcgagaccg ggtagaagag cagagcgaag acgagagcga gggcgacgaa
      541 gatacgctcc gaggtcccgc cggagacgac ccggtactcg tcgcggcccg gaaagacggc
      601 tatcaggcga gagggccaga agagctggac gccgtccaac gtcatcattt cggcgaggag
      661 gtgagagccg tagccgacga agacggcgag catgggggat gagctaccta gggtccacca
      721 cggggggaag ccaagcaaga tggtggcgag cgccagggct agcagcgagt gcaggaagct
      781 accatgcccg aagaggcggt tgaagtcatc agagatcgag cccagctggt agccgagcca
      841 gctcccgggg tgatcggcgt cgggcaggag cgaggtcgca gcggcggcga ggaccgccgg
      901 ggtctcgtag cggacgtcga ggacggccga ggttgcgaac cagcagcacc cggcgaagac
      961 gaggtgtgtc gccgccttca ctcgtccctt ggcctctgca tcttgaagaa gaaagggcgt
     1021 agaccgtaca tgagggcgaa gaagatgccg gcgaggatgt agaggtcctg gtcgtcgagt
     1081 ccgagcgaga tgtaacggac ggccatggcg agggcggcga agacgaagat ggcgggggtc
     1141 atgtcgatgt agcgcctctc gtcctgcagg cgcaactcct cgacctcggc gccgaggcgc
     1201 tcgagggccg ttcgtcccgt gccggcgagg cggccctcga ccacgacgcg gcgccccttg
     1261 agcaggggtc cgaggatgtg ctctatctca cccatcgagg agcggtagag ggcgcgccgc
     1321 aggcccgcct cgtccacctc tctgccgcga acctctccga cctgcctgga ctcgaactcg
     1381 ccggcgcgcc aggccgcgag ggccaggtgc accagcgccg ccttcctgga gccggcaggg
     1441 atcctgaatc ctccggtctc ttcgccctcg gggccatcac ggatccggtg cccggaaacc
     1501 gagagatccc gtcctaaacc gggccgcccg ccgccggggg gtgggcgacg ctcgcctctc
     1561 cccttgcccg atgacgtgtc gaaatccacc agccccttcc tcccgctgcg tgagggaaac
     1621 actcttcata aatggtctct cactcgcgga tacgctctct cttagcgccg gcaatggcct
     1681 tccctgcgga ccgcttcgag gtcggggtcg cgccggtcga gaagcacccg cacgaggccg
     1741 tcctcgttcg ccaggacgcg ccgcctcacg cttcgctcca cggctccggg aagctcgggg
     1801 aggccttcca gagcgatgcc gtagtcgagc ggaccaccgc caggtgcgtc tacgacccgt
     1861 actccaaatc ccagagcccg gagctccagg tatagaggcc gggcaacgtc tgcgtgatct
     1921 ctcagcttct ggcctctccg aagcaccggc gtgtctcagc cgcctctccc cggacgtcct
     1981 tcatgcctgc tctctatgct ggacgccgcg aagcgtcctt ccttgctctg gcgcacgagg
     2041 tgagagagta tctcctccag gtccaacgcc ccgctacgga ggatcgatac gttccgcgcg
     2101 gagagtgctt cggctgtcac actagccgag tcagatacga ggcgtaccgg gctcccaccc
     2161 tcgacttcga cgactcccgg aacgtcttcc cccggctccc tctccaccca gaaggtcttc
     2221 cattgttcga ggagagcatc cttctcgtag aggccgagga agtctccgtc cgcgagaagg
     2281 accacgtagt cggcgatgcg cctcacttct tccatgacgt gggtcgccag tacgaccgtc
     2341 ttcacgccat cgttcatgaa ccgcgagatg tcctcgagca tctccctgcg ggcgaatggg
     2401 tcgacgccag cggacggctc gtcgaggagg aggagctccg agccggtggc cagcgccaga
     2461 gcgaagacga gtcggcgctg caac
//
LOCUS       DASTUV010000354         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_680593, whole genome shotgun sequence.
ACCESSION   DASTUV010000354 DASTUV010000000
VERSION     DASTUV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680593"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFT03_07100"
     CDS             complement(<1..993)
                     /locus_tag="VFT03_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU4847949.1"
                     /translation="MIERIVIFGATGDLTGRYLIPALAKLYEAGRLPSGIKVTGIDRR
                     EWKNGKNGFRRLVLDSLERHAGGVSANARARMAVEVLEYRQADVTDGESVISALGPLT
                     GPIIAYLALPPAVFTPTLEALKKANLPGGSRVAIEKPFGENLESAQKLNRLLHGFLPE
                     QAIFRVDHFLAWQPFPTIMGLRFANGVFEPLWDRNYIERVEIVWDETLALEGRAGYFD
                     HTGAFKDLIQNHLLQLLCLTAMEAPTALHRRNLRDRKVDLLRAIRRFSPEEAQRHTVR
                     ARYGPGRIADRHIPAYADEEGVDPDRGTETFAQVTLMIDNLRWAGVPFLLRGGKA"
     gene            complement(1022..>2682)
                     /locus_tag="VFT03_07105"
     CDS             complement(1022..>2682)
                     /locus_tag="VFT03_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEU4847950.1"
                     /translation="REKEPVHIEKMLAFYTSRDAAINEPLVNAGKAVNRYGTFDEAFE
                     RHVRAWDELWELCDVRVPRDDRVQLLLRLHISHILQVCSPHTADLDAGVPARGLNGEA
                     YRGHIFWDELFIYPFLNYRLPEITRELLMYRYRRMDEARAAAREAGYEGTMFPWQSGS
                     DGQEETQTLHLNPKSGKWEPDLSHNQRHVSADIFYNIWRYYESTGDFEFLLDHGAEMM
                     LEIARFWSSIAHFNPERDRYEIHGVMGPDEFHEKYPDSEEEGLRNNAYTNVMVAWLSD
                     IVLKVLELLPERRREALRARIGLTDEEVERWKEMSRKMFVPFHGDGLISQFEGYEDLE
                     ELDWDHYREKYGNIQRLDRILRAEGDDPDKYKLTKQADAVLLFFLFPEDELKRIFERL
                     GYDYAPEMVRENVEYYDRRTSHGSTLSFIAHAGILADLDPESSWERYMVALESDVGDV
                     QGGTTQEGIHMGVMAGTLDLIQRGYLGTDIRDGALHFDPKPVGNLDGLSFPMQFRGTP
                     LEVELDDGRLTVAAQSDDIGQPIQVRVGDESREIKPGESYTFGA"
BASE COUNT          418 a          863 c          874 g          527 t
ORIGIN      
        1 ggccttgccc ccgcgcagca agaacggcac tcccgcccag cgcaggttgt cgatcatgag
       61 cgtgacctgg gcgaaggtct cggttccccg gtccggatcg actccttcct cgtcggcgta
      121 ggccgggatg tgcctgtccg cgatccttcc gggaccgtag cgggcgcgca cggtatgccg
      181 ttgcgcctcc tcgggggaga agcgccggat ggcccgcaag aggtccacct tgcggtcgcg
      241 caggttgcgc ctgtgcaggg cagtaggggc ctccattgcc gtgaggcaca ggagctggag
      301 gaggtggttc tggatcaggt ccttgaaggc tccggtgtgg tcgaagtagc cggctctgcc
      361 ctccagagcc agggtctcgt cccagacgat ctcgacgcgt tctatatagt tgcggtccca
      421 gagcggctcg aagacgccgt tggcgaagcg cagacccatg atagtgggga acggctgcca
      481 ggcgaggaag tggtccacgc ggaagatcgc ctgctccgga agaaacccgt gcaacagcct
      541 gttgagtttc tgggccgatt cgaggttctc gccgaagggc ttctcgatgg cgaccctgct
      601 acccccaggc aggttggcct tcttcagggc ctcgagcgtc ggggtgaaca ccgcgggcgg
      661 cagggccagg tacgcgatta tcggccccgt cagcggcccc agggccgaga tgacgctctc
      721 gccgtccgtc acgtcggcct ggcggtactc caggacctcg acggccatcc tcgcgcgggc
      781 gttcgcggat acgccgccgg cgtggcgctc caggctgtcg agcaccaggc gcctgaagcc
      841 gttcttgccg ttcttccact ccctcctgtc gatcccggtg accttgatgc cgctcggcag
      901 cctgcccgct tcgtagagct tggccagggc ggggataagg taccggcccg tcaggtcccc
      961 cgtggccccg aagattacta tgcgctctat cacgaacctt cctccattac cggggctgtt
     1021 atcaggcgcc gaaggtgtaa ctttcgccgg gtttgatctc ccggctctcg tcgccgaccc
     1081 ggacctggat aggctggccg atgtcgtcgc tctgagcggc gaccgtgagc ctgccatcat
     1141 cgagctctac ctcgagcggc gtcccgcgaa actgcatggg gaaggacaac ccgtccaggt
     1201 tgccaacggg cttggggtcg aagtggagcg ccccgtcgcg gatgtccgtc cccaggtagc
     1261 cgcgctgtat caggtccagc gtcccggcca tcacgcccat gtggatgcct tcctgggtgg
     1321 tgccgccctg cacgtcgccg acgtcgctct ccagcgccac catgtagcgc tcccacgagc
     1381 tctcggggtc gaggtcggcc aggatgccgg cgtgagcgat gaagctcaag gtggagccgt
     1441 gggaggtcct cctgtcgtag tactcgacgt tctcgcgcac catctcgggc gcgtagtcgt
     1501 aaccgagccg ctcgaagatc cgcttcagtt cgtcttcggg gaaaaggaag aagagcagca
     1561 cggcatcggc ctgcttggtc agcttgtact tgtccgggtc atccccctcg gcgcgcagga
     1621 tgcggtcgag acgctggatg ttgccgtact tctcgcggta gtggtcccag tccagctcct
     1681 ccagatcctc gtaaccctcg aactggctga tgagcccgtc gccgtggaac ggtacgaaca
     1741 tcttgcggct catctccttc cacctctcga cctcctcatc ggtgaggccg atcctggcgc
     1801 gcaaagcctc tcgacggcgt tcggggagca actcgagtac tttcaggacg atatcggaga
     1861 gccaggccac catcacgttg gtgtaagcgt tgttccgcag tccctcttct tccgagtccg
     1921 gatatttctc gtggaactcg tccgggccca tgacgccgtg gatctcgtat cggtcccgtt
     1981 cggggttgaa gtgggcgatg gaggaccaga agcgtgcgat ctccagcatc atctcagcac
     2041 cgtggtcgag caggaactcg aagtcccctg tcgactcgta gtagcgccag atgttgtaga
     2101 agatgtccgc gctcacgtgc cgctggttgt ggctcaggtc cggctcccac ttccccgact
     2161 tggggttgag gtgcaacgtc tgggtctcct cctggccgtc ggagccgctc tgccagggga
     2221 acatcgtccc ctcgtagccg gcctctctgg ccgctgcgcg ggcctcgtcc atgcgacggt
     2281 agcggtacat taagagctcg cgcgttatct ccggcagccg gtagttgagg aagggataga
     2341 tgaagagctc gtcccagaag atgtgtccgc ggtaggcctc accgttcaag ccccgggcgg
     2401 ggacgccggc gtcgaggtcg gcggtgtgcg gcgagcagac ctggagtatg tgcgagatgt
     2461 gcagccggag caggagctgg accctgtcgt ctctggggac gcgcacgtcg cagagctccc
     2521 acaactcgtc ccatgcccgc acatggcgct cgaaggcttc atcgaacgtc ccgtagcggt
     2581 taaccgcctt cccggcgttg accagcggct cgtttatggc ggcgtcgcgc gaggtgtaga
     2641 aggcgagcat cttctcgatg tgcacgggct ccttctctct ca
//
LOCUS       DASTUV010000355         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_576190, whole genome shotgun sequence.
ACCESSION   DASTUV010000355 DASTUV010000000
VERSION     DASTUV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576190"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            838..1197
                     /locus_tag="VFT03_07110"
     CDS             838..1197
                     /locus_tag="VFT03_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847951.1"
                     /translation="MPNRANRDERAREPSLRKLFLGSHVRAEREEKVLRYIIHRVNES
                     APLHDVVQEDYVRRNCTQAEIDEIVNAPELVHACRENLWQTFKSGELDPLQPRHASPR
                     GPSAGREDGGDTLPPGA"
     gene            1427..1843
                     /gene="msrB"
                     /locus_tag="VFT03_07115"
     CDS             1427..1843
                     /gene="msrB"
                     /locus_tag="VFT03_07115"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4847952.1"
                     /translation="MTDTPRKVQKSEEQWQDELTSEQYRVTRMKGTERAFTGEYWDHK
                     GDGVYACVCCGSPLFSSETKYDSGTGWPSFWEPLDENVETEEDRSLFMRRIEVHCAAC
                     GAHQGHVFDDGPAPTGKRYCINSAALRFEPETAEEE"
     gene            complement(1873..>2837)
                     /locus_tag="VFT03_07120"
     CDS             complement(1873..>2837)
                     /locus_tag="VFT03_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU4847953.1"
                     /translation="SDLYSLGVVLYEMLTGEPPYSADNPLATAMKHLDEPPRHPREAN
                     PAVPEGLDALTTKLLAKDPGDRYASAAVLAEDLRRVRDGLPPLAAGAGQQSTAQMPRD
                     TGEAHTTPTLVAPGRGQGAGPRTRRALLPLAVLLLGAVLLGGLAWALARDASTGNTSG
                     AERVEVPGVVGLSVEEARGRLREADLDLGSQSESVSDEAAAGEVIRQTPPAGNQAQRG
                     SDVDVVVGTGPPAVEPTTPSSSASSSASSSASSTATPTAPASSSPANNEAPKQATEEP
                     QRQVEKPAKQRERAQQKAVKQPTENRPSPGNRGKHGKGKKGKGR"
BASE COUNT          441 a          933 c          956 g          507 t
ORIGIN      
        1 cgatcccgcc aagaactcgg gagagcccgc gcctcccaag tcagcacgct acgccctctt
       61 cgagggctcc gctggtcagc acgtcccctt cggggacttg tcagcatgcc gcctggctac
      121 gccagacgcc ttgtcagcac gctcgggcct agcggccctc gcttctcagc atttcagctt
      181 tcactgccgc taggcacttg cccggatcac gcttggagag acattctatg ggcaaatcct
      241 tggactggcg cggggaagca ccatatcgcg tcctccttgc ttttagctga caagcctccg
      301 accgaaggga ggcctgctga caagcgaagg ccgcaggccg gagcgtgctg aaatgctcgc
      361 gtggtttaaa atacagacgt catggggctt tctctatcct ggcggttctt tcgcggcttg
      421 gcgctcgtat cggtcgccgc cctcctgctc ggcgccgggg tctttctttc ctggtccgct
      481 accggaccct gggtcaccac cgtggcgggg tctcatacgg cccagggtgt agacggacac
      541 ccgccgagcg ggagccagct cggcgtgttc ggggcggaca tcgaggaggg cgataaaggc
      601 ccggtgaact cagtcctcct gacggtgctc ctgctcgcgt cctttttcgg tggtacagcc
      661 ggatggcaac tcgcttgtga cctgaggcaa ccggcgcctt gtgtcccagg ccggccttcg
      721 ttcgaggccg ctcgtgagga cgcacccttc cttagcgtgt tccggctcta ggaggctcac
      781 ggccccgccc ggggcttata ggagccagcg caccagggac acgaaaggag cgtccacatg
      841 cccaacaggg ccaacaggga cgagcgcgcc cgtgagccgt cgctgaggaa gctattcctc
      901 ggtagccacg tccgcgccga gcgggaggag aaggtactcc ggtacatcat ccaccgcgtg
      961 aacgagagcg cgccgctgca cgacgtagtg caggaggact acgtacgccg caactgcact
     1021 caggccgaga tagacgagat cgtcaacgcc cccgagctcg tccacgcctg cagggaaaac
     1081 ctctggcaga cgttcaagtc aggggagctg gatccgctcc agccgcgcca cgcctcgccc
     1141 cgtggcccgt cggcgggccg tgaggatggc ggcgatacgc ttccgccggg cgcgtgatga
     1201 cgcctcgatg agcgggccgc aggacagatg cgaccacagt ggtccaaccg aggtcgagcg
     1261 ggcggaagga tgctaccgcg cgcggtgtac ggcttgcggg gagagcgggc cggcgcggaa
     1321 gacagccgaa gccgcccgca aggcgctcgt ggaactgggc gcgcgggatg gaacccgcgg
     1381 aggctcgtgg gtcggaaagc actagacccg tgctagaatc agccgtatga cagatacgcc
     1441 gagaaaggtc cagaagtccg aagagcagtg gcaggacgag ctcacctccg aacagtaccg
     1501 cgtaacccgc atgaagggca ccgaacgggc tttcacaggc gagtactggg accataaagg
     1561 cgatggcgtc tacgcgtgcg tgtgctgcgg atcgccgctc ttcagttccg agaccaagta
     1621 tgactccggg acggggtggc cgagtttctg ggagcccctc gacgagaacg tcgagaccga
     1681 agaggaccgc agcctcttca tgcgccgcat cgaggtccac tgcgcggctt gcggggcgca
     1741 ccagggccac gtcttcgacg acggtcccgc gccgacaggc aagcgctatt gcatcaactc
     1801 ggccgccctg cgcttcgagc cggaaaccgc agaagaggag taacttcgag tacgggggtc
     1861 gcggacccgt tcctatctgc ctttgccttt cttgcctttc ccgtgcttgc ctctgtttcc
     1921 gggagatggc ctgttctctg tcggctgttt taccgctttt tgctgcgccc tctccctttg
     1981 cttcgccggt ttctcaacct gtctctgggg ttcctccgtg gcctgttttg gtgcctcgtt
     2041 gttcgcgggc gaggaggagg ccggcgccgt cggcgtggct gtggacgatg ccgacgacga
     2101 cgctgaagag gaggcggagg atgacggcgt ggtgggctct acggcgggag ggcctgtgcc
     2161 gactacgacg tccacgtctg agccccgctg tgcctggtta cccgcgggtg gggtctgcct
     2221 tatcacctcg ccggcggctg cctcgtcgct gacgctctcc gactgggagc cgagatccag
     2281 gtccgcctct ctgagacgtc ccctggcctc ctccaccgac aggcccacta cgccgggcac
     2341 ctccacccgc tccgcgcccg atgtgttccc ggtcgaggcg tctcgcgcca gcgcccacgc
     2401 gaggccgccg agcagtactg cgcccaggag caggacggcc aggggtagca aggcacgcct
     2461 ggtcctgggt cctgcaccct gtcctctccc tggtgcgacg agggtggggg tcgtgtgggc
     2521 ctcgccggtg tcccggggca tctgcgcggt cgactgttgc cctgctcccg cggcgagggg
     2581 cgggagcccg tcgcgcaccc gcctgaggtc ttcagcgagc actgcggcgc tcgcgtagcg
     2641 gtccccgggg tccttcgcca gcagcttcgt ggtcagggcg tccagcccct ctggtaccgc
     2701 ggggttcgct tcccggggat ggcgcggcgg ctcgtccagg tgcttcatgg ccgtggcgag
     2761 ggggttgtcc gcgctgtagg ggggctcgcc cgttagcatc tcgtagagga ccacgcctaa
     2821 agagtagagg tcgctct
//
LOCUS       DASTUV010000356         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_558498, whole genome shotgun sequence.
ACCESSION   DASTUV010000356 DASTUV010000000
VERSION     DASTUV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558498"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..543
                     /locus_tag="VFT03_07125"
     CDS             358..543
                     /locus_tag="VFT03_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847954.1"
                     /translation="MYRMNEVAFWNGRRHELVREAEDGRLAWRLRAASPKRVARFRSG
                     LFERTPVHLRAQSSGRA"
     gene            560..1987
                     /locus_tag="VFT03_07130"
     CDS             560..1987
                     /locus_tag="VFT03_07130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4847955.1"
                     /translation="MELRVFTSGGAETALENSVIGAFGTRLHGELLRPGDAGYEGARK
                     VWNGMIDRRPALIARPRSVADVINSVNFARENDLLVAVRGGGHNVAGTGTVDGGLLID
                     LSRMKGIKVDPERCTARVEPGVTIGELDRKTQAFGLAAPMGVVSETGIAGLTLGGGLG
                     WLRREHGLSSDNLISVDIVTADGRFLSASEDENPELFWGVRGGGGNFGVVTSFEYRLH
                     PVGPEVMFGFVFYPGDRTGEVLRFMNEYMAQAPDEVSPLSVLGRVPEAEMFPKDWHGE
                     QFVAVLAMYSGEVEEGERVLAPLRELGEPIVDFSGPKPYVEAQAILDEDYPEGGRYYW
                     KSQNVNRLGDEEIEHLIAHAEAAPSEHSTIDVWYQGGAMGRIGAAESAFGDRRAPVLL
                     GIEANWEEPRDDEANVAWARECVSDMRRFSDGGMYLNFPGFMEEGQKLMQEAYGRNYE
                     RLVALKDEHDPTNLFRLNQNIRPTA"
     gene            2055..2723
                     /locus_tag="VFT03_07135"
     CDS             2055..2723
                     /locus_tag="VFT03_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847956.1"
                     /translation="MTTQDSDQTNEAQAPELGATLLHVAWLAVILGLVMEVLLLVLGG
                     AVGEALGLKPFVADLVRNVTWSVFVCAGLAVGTTIVKARAPLMGVLGLVSGPLAFEIS
                     RAFHKGALEALAITGGGGSAVSPVLVAAVKGIEYGCLGLGVGWVSQRRWGGAAAHVAV
                     GLLVGLTFGGVQLALAAGATPPPPTSDLFVAGVNEVLFPVGCSLVLFSAGALSKKTAS
                     LSSR"
     gene            complement(2768..>3247)
                     /locus_tag="VFT03_07140"
     CDS             complement(2768..>3247)
                     /locus_tag="VFT03_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEU4847957.1"
                     /translation="AREVLVVPENRPIDGILDEFQRQELQMAIVIDEWGSFEGLFTLE
                     DIIEEIVGEIRDEFDEEEPAVRQLSDGFYSIDGRIPIGVVNEALGSEFESEDFDTIGG
                     LVLGSLGRVPEVGDEIRFDGYLLHVDEVDGTRVAQVVARELPEEETTSSRKEEDSEG"
BASE COUNT          528 a         1037 c         1113 g          569 t
ORIGIN      
        1 ctcgtaacga ctttggtact agattatcgc cccgccgaag gtccatagca atattcgttc
       61 ctacgcccga tggccgcgga tttaccggct ccgtgctcgg cgagaaccgt cgcgctctgg
      121 gcgctctggc ggccgtgttc ccccaggatg cgctacgagg tgagggatgg cgcctaccag
      181 gtgcgcgcac cgctgcgcga ggtagtggcc tgagctacgc ggcctgcctg gactttagtc
      241 ctatgccgtt actccgacga aggtccccgg tgacaaccgt tctttatccc gatgctcgcg
      301 ccccggttcg gcgatttact cgttgcacag acaaaagacg agcgaaggag gcaggcgatg
      361 taccggatga acgaggtggc gttctggaac ggacgccgcc acgagttggt gcgcgaggcc
      421 gaggatggac gcctggcctg gcgcctgagg gcggcgagcc cgaagcgggt cgcccggttc
      481 cggagcgggc tcttcgagcg aaccccggta cacctccgcg cgcagagcag cggacgggca
      541 tgaggagcgg gagtatctga tggagcttcg cgtattcacc agcggtggag ccgagaccgc
      601 gctggaaaat tcggtgatcg gggcgttcgg gacgcgcctg cacggcgagt tgctgcgccc
      661 cggcgatgcg ggctacgaag gggcccgcaa agtctggaac ggcatgatcg acaggcgtcc
      721 tgcgctcata gcgcggccga ggagcgtagc cgacgtcata aactcggtaa acttcgcccg
      781 cgagaacgac ctgctcgtgg cggtgcgcgg cggcggccac aacgtcgccg gcaccggaac
      841 ggtagacggt ggcctgctga tcgacctctc caggatgaag ggtatcaagg tagaccccga
      901 gcggtgcacg gcccgcgtcg agcccggcgt caccataggc gagctggaca ggaagacgca
      961 ggccttcggc ctcgcggcac ctatgggggt tgtctcggag acggggatcg ccggtctcac
     1021 tttaggcggc ggcctcgggt ggttgaggcg cgagcacggt ctctcctcgg acaacctcat
     1081 ctcggtggac atcgttaccg cggacggcag gttcctgagc gccagcgagg acgagaaccc
     1141 cgagctcttc tggggagttc gcggcggcgg gggcaacttc ggcgtcgtga ccagcttcga
     1201 ataccggctc catcctgtag gacccgaggt gatgttcggc ttcgtcttct accccggcga
     1261 ccggacgggg gaagtcctgc gcttcatgaa cgagtatatg gcgcaggccc ccgacgaggt
     1321 gagccccctg agcgtcctcg gccgggtccc cgaggccgag atgttcccca aggattggca
     1381 cggcgagcag ttcgtcgcgg tcctggctat gtactcgggc gaggtcgagg agggcgagcg
     1441 ggtgctcgcg cccctgcgcg agctcggcga gccgatcgtg gacttcagcg gcccgaagcc
     1501 ctacgtcgag gcgcaggcga tcctcgacga ggactatccc gaaggaggtc gctattactg
     1561 gaagtcgcag aacgtgaacc gtctcggtga cgaggagatc gaacacctca tcgcccacgc
     1621 cgaggcagcg ccgtcggagc actccactat cgatgtctgg taccagggcg gggctatggg
     1681 ccggatcggg gccgcggaga gcgccttcgg cgaccggcgc gcgcccgtac tgctcggcat
     1741 agaggccaac tgggaggagc cgcgcgacga cgaggcgaac gtcgcctggg cgagggagtg
     1801 cgtctccgac atgcggcgct tctccgacgg cggcatgtac ctgaacttcc caggtttcat
     1861 ggaggagggg cagaagctga tgcaggaagc ctacggccgg aactacgagc ggctcgtcgc
     1921 gctcaaggat gagcacgatc ctaccaacct cttccgcctc aaccagaaca tcaggcccac
     1981 cgcctgacgg tcgtatagaa aatcccggct cgctcccgcg ataacgagag gttagacttg
     2041 aggcgagcga cgccatgacc acacaggatt cggatcagac gaacgaagct caggcgcccg
     2101 agcttggagc cacgctcctg cacgtggcgt ggttggcggt catcctcggt ctggtgatgg
     2161 aggtgctgct gctcgtgctc ggcggggccg tgggggaggc gcttggcctc aagcccttcg
     2221 tcgccgatct ggtcaggaac gttacctggt ctgtcttcgt ctgcgcgggt ctcgccgtcg
     2281 ggacgacgat cgtcaaggct cgcgcgccgc tgatgggcgt cctgggcctg gtctccggcc
     2341 cgctcgcgtt cgagatctcg cgcgcgttcc acaagggcgc gctggaagcc ctcgcgatca
     2401 caggtggcgg gggcagcgcc gtctccccgg tgctggtcgc cgctgtcaag gggatcgagt
     2461 acgggtgcct tggcctgggg gtcggctggg tgagccagcg ccggtggggc ggcgcggcag
     2521 cgcacgttgc ggtgggacta ctggttggcc ttaccttcgg gggcgtgcag ctggccctcg
     2581 cggcgggagc cacgccgccc ccgcccacct ccgacctctt cgtcgctggc gtcaacgagg
     2641 tcctcttccc tgtcggctgc tccctggtgc tcttctccgc cggggcactt agtaagaaga
     2701 cggcgagcct gtcaagccgg taataggcga acgtcgcctc ctccaagcga cgcgcccctg
     2761 aagcctctta cccttcggag tcttcttcct tccgggagct tgtcgtctcc tcttcgggca
     2821 gctccctggc gaccacctgg gctacgcggg tgccgtcaac ctcgtccacg tgaagcaggt
     2881 agccgtcgaa gcggatctcg tctccgacct cggggacgcg accgaggctg ccgagcacca
     2941 ggccgccgat ggtatcgaag tcctcgctct cgaactcgga gccgagggcc tcgttcacga
     3001 cccctatggg gattcggccg tctatcgagt agaagccgtc agatagttgg cggaccgccg
     3061 gctcctcctc gtcgaactcg tcccggatct cacccactat ctcctcgatg atgtcctcca
     3121 gggtgaagag cccctcgaag gagccccatt cgtctatgac tatggccatc tgcagctctt
     3181 gcctctgaaa ttcgtcgagg atcccgtcta tggggcggtt ctcggggacg acgaggacct
     3241 cgcgcgc
//
LOCUS       DASTUV010000357         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_553782, whole genome shotgun sequence.
ACCESSION   DASTUV010000357 DASTUV010000000
VERSION     DASTUV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553782"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1069
                     /locus_tag="VFT03_07145"
     CDS             305..1069
                     /locus_tag="VFT03_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEU4847958.1"
                     /translation="MPEERRRRIARLVRESGSVTVSLLESEFGISPMTVRRDLMALEE
                     EGRLRRIHGGAVLPEFARHEDSFWYRLEEEAAAKERLAEVAVGLLEAGDSVFLDCSTT
                     TYYLARRIMREGPRVTLLTNSVPVMELFMKNETPKTDIIGVGGLMRKLTLSFVGPHAV
                     SMISAHSADRAFVSVKGVTRDGYLTDPDLMEAEVKRAMIEHSEEPVLLVDGGKLEKRG
                     LNVITHASGLSRALVAEAKEERLAPLVRAEVEVQLV"
     gene            1074..1436
                     /locus_tag="VFT03_07150"
     CDS             1074..1436
                     /locus_tag="VFT03_07150"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEU4847959.1"
                     /translation="MNEGARVWLVPDGFIPEESSGSQESHEAVCVLNTSGEEAQLSIS
                     FYFEDREPVKGVELIVPPERTRHARTDELDGAEIPRGVPYAMRVESSVPVVVQCSRMD
                     TTQAALSLMTAVAHPVER"
     gene            1433..>2386
                     /locus_tag="VFT03_07155"
     CDS             1433..>2386
                     /locus_tag="VFT03_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEU4847960.1"
                     /translation="MSKRADFLAVDVGAESGRVFLGCFDGQRVDLREVHRFANVPVRV
                     ADGLHWDVLRIMHEVNEGLRRAVREGRELASVGVDTWGVDFALLDRDGALVSNPYHYR
                     DGRTEGMMKRAAERVPAEEVYRTTGIQFIPLNTLYQLLALEDSPLLEAAETLMMMPDL
                     IGYWLTGEKACEYTNATTTQLYDLDAADWAWELARKLGIPERIFPEIIAPGTQLGALL
                     PEVRVETGLGEVPVTATATHDTASAVVAVPADGPFAYISSGTWSLVGVETERPVTTQG
                     ALRHNFTNEGGFGGRVRLLRNVMGLWLLQECRRTWGRAGSDY"
BASE COUNT          452 a          706 c          832 g          396 t
ORIGIN      
        1 ccgagcccca gaaagcctac cgcgtccacg catccggccg ccagagagag catcaccagc
       61 atcgcgtttc gtaccgtcgt gcgcgacgta ccacccaaac ccctcgactc gtcgccgcga
      121 cgctccataa acctccctcg aaagctcatc cattgccgga agatgccaga atacttctcc
      181 atccgctacc aaccccaacc ctcttgcgcg aaagcgtagc ttctgctact actgatagaa
      241 gttgagtgat tgggtgggga aaataggaaa aaactaccag gaaatatcag aaaaacagac
      301 gacgatgcct gaggagaggc gtcggcggat agcgcggctt gtcagggagt cggggagtgt
      361 cacggtctct cttttggagt cggagttcgg gatctcgccg atgacggtca ggcgggatct
      421 catggccctg gaagaggagg gccggctgcg caggattcat ggaggggctg tcctcccgga
      481 gttcgcgagg cacgaggact cgttctggta ccgtctggag gaggaagcgg cggccaagga
      541 gcggctggcc gaggtcgccg tcgggcttct ggaggctggc gactctgtgt tcctggattg
      601 ttccaccacg acgtactacc tggcgcgcag gatcatgaga gagggaccgc gggttacgtt
      661 gttgaccaac tccgtgccgg tgatggagct gttcatgaag aacgaaacgc ccaagacgga
      721 catcatcggc gtaggtgggc tcatgaggaa gctcaccctc tccttcgtgg gaccgcacgc
      781 ggtaagcatg atctcggctc actctgcgga cagggccttc gtcagcgtca agggggttac
      841 ccgcgacggc tacctgacgg acccggatct catggaggcc gaggtcaagc gcgccatgat
      901 cgagcactcc gaggagcccg tcctgctcgt ggacggcggc aagttggaga agcggggact
      961 gaacgtcata acgcacgcct ccgggctctc gagggcgctg gtggcggagg ctaaggagga
     1021 acggctggcg ccgctcgtac gcgctgaggt ggaggtgcaa cttgtctagg tagatgaacg
     1081 agggcgccag ggtgtggctc gtccccgacg ggttcatccc cgaggagagt tcggggtcgc
     1141 aggagagcca cgaggccgtc tgcgtgctca acacctcggg ggaggaggcg cagctctcga
     1201 tctccttcta cttcgaagac agggagcccg tcaaaggtgt ggagctcatc gtcccgccgg
     1261 agcgcacccg gcacgcgagg accgacgagc tggacggggc cgagataccg cgcggcgtgc
     1321 cgtacgcgat gcgggtggag tcgagcgtcc ccgttgtggt ccagtgctcg cggatggaca
     1381 cgacccaggc agccctctcg ctgatgaccg cggtggccca cccggtggag cgatgagcaa
     1441 gagggccgac ttcctcgcgg tggacgtcgg ggcggagagt ggtcgggtat ttctcggttg
     1501 cttcgacggc caacgcgtgg atctccggga ggtccatcgt tttgcgaacg tgccggtacg
     1561 cgtggcggac ggcctgcact gggatgtgct ccggataatg cacgaggtga acgaggggct
     1621 gcgcagggcg gtccgggaag gtagagagct ggcgagcgtc ggggtggata cctggggggt
     1681 ggacttcgcc ctccttgacc gggacggagc gctggtctcc aacccgtacc attaccgcga
     1741 cgggcgcacc gaaggaatga tgaagagggc agcagagcgg gtgccggcgg aggaggtcta
     1801 ccgcacgaca ggcatccagt tcattccgct caacaccctc taccagctcc tcgccctcga
     1861 ggactcgccg ctgctcgaag ccgcggagac gctcatgatg atgcctgacc tcatcgggta
     1921 ctggctcacc ggcgagaagg cctgcgaata cactaacgcc acaacgaccc agctctacga
     1981 cctcgacgcg gcggactggg cctgggagct ggcacggaag ctgggcatcc ccgagcgcat
     2041 cttccccgag atcatcgctc ccgggacgca actcggagcc ctgttgccgg aggtaagagt
     2101 agagacgggg cttggagaag tgccggtgac ggcgaccgcg acgcacgaca ccgcctcggc
     2161 cgtcgtcgct gtacctgccg acggtccctt cgcgtacatc tccagcggca cgtggtcgct
     2221 cgttggggtc gaaaccgaga ggcccgtcac cacgcagggg gcgctgcgcc acaacttcac
     2281 caacgagggc gggttcggtg gtagggtgcg gctgctgagg aacgtgatgg ggctctggct
     2341 cttgcaggag tgtcgcagga catgggggcg ggccggaagc gactac
//
LOCUS       DASTUV010000358         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_466468, whole genome shotgun sequence.
ACCESSION   DASTUV010000358 DASTUV010000000
VERSION     DASTUV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466468"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="cydB"
                     /locus_tag="VFT03_07160"
     CDS             complement(<1..846)
                     /gene="cydB"
                     /locus_tag="VFT03_07160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU4847961.1"
                     /translation="METIWFICVAFMISMYVLLDGFDIGAGAIHPFAAREDRERRTII
                     RAIGPVWDGNEVWLIAAGGTLFFAFPVLYASSFSGFYLPLIIVLWLLMIRGLSIELRG
                     HIESPLWASFWDAMFFIGSALLAVFFGAALGNVVRGVPLNESGYFFQPLWTDFSPFSR
                     TPGILDWYTVLIGLLALTTLVVHGANFIALKTENDLNARSRGISRLFALVTAALTALA
                     TLATFWVSPWMLQSFNDRPYGYVLPLVAIAGLAGTVYFNLRRQDRAAFLSSSAYILGM
                     LTSTVF"
     gene            complement(860..2206)
                     /locus_tag="VFT03_07165"
     CDS             complement(860..2206)
                     /locus_tag="VFT03_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU4847962.1"
                     /translation="MEDALTVSRLQFAFTVTYHYLFPMFTMGLALLIFVLKSVYMRNR
                     DELYNVAARFWGKIFAITFVMGVVTGIPLEFQFGTNWAAFSRATGDIIAQTLAMEGAF
                     AFFLESAFVGLFLFGERRFGQRVHWFSSLMVFVGTWASGYFIITTNAWMQHPVGYRVL
                     ENGTIQLDNYWAVLLNSWMWAQFAHNMGGAVVCGAFVMAGLGAFYLLANRHEEQGRIF
                     VKVGVIAGVLASLWMLFPTGHFSSEQVAEHKPVALAAMEGLFESERAAGIVLVGQPDV
                     EGRSIENPIVVPRALSYLIYQNWNSEVKGLGAFPKKNWPDNIPLLYYSYHVMVGLGTI
                     FITIMVLAAFLLWRGRLYGSRWMLWVLMLSIPFPFIANTAGWFTAELGRQPWLAYGLF
                     RTSEGVSPQVSSGSVLFTLIGFAGMYLIMGLLYIVLMVREVDHGPETLESSEAPTS"
     gene            complement(2315..4045)
                     /gene="cydC"
                     /locus_tag="VFT03_07170"
     CDS             complement(2315..4045)
                     /gene="cydC"
                     /locus_tag="VFT03_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEU4847963.1"
                     /translation="MFLRLLGFLQPYSWQVALAILLGVATVVSNVGLLATAAYLISAA
                     AIVPYLSMLVIPVYLVRLFSVSRAASRYAERLTSHDVTFKLLGNLRTWFYARLEPLAP
                     ARLARYRSGDLLSRVVKDVEELENVYLRVVSPLVVAIVVSLITFLVFHAFGTAMALAA
                     AGFLVVTGVGAPLLVRLLSRGLGRRQLELRAELGARVVDDVRGIQDLLAFGAEERERR
                     ELSTLAGMLDRAQGRTALVTGLQSALADLMAGLALVAILVLAAPLVAAGEVRGVYLAF
                     LALVMVGSFEAVVPLGSALGSLGRSVHAGERLFEVIDAEPEVVDPPEPLKAPCDVTLE
                     FDRVSFCYGEGLPPALEDVSFALRPGCRVAIVGPSGSGKSTLVSLALRFWDPVSGEIR
                     LGGRDVRSYVQEELRARIGFVSQNTHVFNDTLRANLLIADPQATDVALHGALARARLE
                     ELVERLPRGLDSYVGEQGLALSGGERQRLAIARVLLKDAPLLVLDEASANLDTITERE
                     LMAEIKDLMRDRSTLILTHRLVTLETMDEILVLEKGRIIERGTHEELVRAEGLYRRML
                     DVQRGMLAAS"
     gene            complement(4048..>4538)
                     /locus_tag="VFT03_07175"
     CDS             complement(4048..>4538)
                     /locus_tag="VFT03_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4847964.1"
                     /translation="VPQRPYLFYGSLLENIRLARPGASQAEVEEAAELAGAADFIRHL
                     PRAYEAQVGERGLLLSGGEAQRVAIARAFLKDAPLLVLDEPTSSLDPESEALIGAAIE
                     RLARGRTTLVVAHRLNTALAADRIAVLDEGRLEDVGTHAELLARGGTYSRLAGAYGDV
                     PA"
BASE COUNT          886 a         1483 c         1499 g          670 t
ORIGIN      
        1 gaagaccgtg ctggtcagca tcccgaggat gtaggcgctc gaagagagga acgcggccct
       61 gtcctgcctc cgtaggttga agtagaccgt cccggctagg ccggctatgg cgacgagcgg
      121 gaggacgtac ccgtagggcc ggtcgttgaa gctctggagc atccagggac tcacccagaa
      181 ggtggccagg gtcgcgagcg ctgtcaacgc ggcggtcacc aacgcgaaga ggcgggagat
      241 cccgcgcgag cgggcgttca ggtcgttctc ggtctttagg gcgatgaagt tcgcgccgtg
      301 cacgacgagc gtcgtgagag cgaggagacc gatcaggacc gtgtaccagt ccaggatgcc
      361 gggggtccgg gagaaggggc tgaagtccgt ccagagcggc tggaagaagt aaccgctctc
      421 gtttagcggg acgccgcgca cgacgttacc cagagcggcg ccgaagaaga cggcgagcag
      481 ggcgctcccg atgaagaaca tcgcgtccca gaacgaggcc cagagggggc tctctatgtg
      541 cccgcgcagc tcgatggaga ggccccggat catcaggagc cagagcacta tgatcagggg
      601 caggtagaac ccggagaagc tggaagcgta gagtacgggg aaggcgaaga acagggtgcc
      661 gccggccgct atcagccaga cctcgttgcc gtcccacacg ggcccgatgg cgcgtatgat
      721 cgtgcgccgc tccctgtctt ccctggcggc gaacgggtga atagcgcccg cgccgatgtc
      781 gaagccgtcc aggaggacgt acatcgagat catgaacgcc acgcagatga accagatcgt
      841 ctccaccact gcctcctttc tagctcgttg gggcctcgga ggactccagc gtctcggggc
      901 cgtggtccac ctcgcgcacc atcagcacga tgtagaggag gcccatgatc aggtacatcc
      961 ccgcgaaccc tatgagcgtg aacagaacgc tccccgagga gacctggggc gagacgcctt
     1021 cggaggtgcg gaagaggccg taggctagcc agggctggcg tccgagctcc gcggtgaacc
     1081 accccgctgt gttcgcgatg aacgggaagg gtatagagag catcaagacc cagagcatcc
     1141 accgcgagcc gtagagcctg ccccgccaca agaggaacgc cgccaggacc atgatggtta
     1201 tgaagatagt ccccagtcct accatgacgt ggtagctgta gtagaggagc gggatgttgt
     1261 ccggccagtt cttcttggga aaggcgccga gccccttcac ctcggagttc cagttctgat
     1321 agatcaggta gctcaaagcg cgcggaacga cgatcgggtt ttctatgctg cggccttcca
     1381 catcgggctg gccgacgagg actatgccgg ctgcgcgctc ggactcgaag agcccctcca
     1441 tcgcggctag cgccacgggc ttgtgctcgg cgacctgctc gctggagaag tggcccgtcg
     1501 ggaagagcat ccagaggctc gcaagcacgc ccgctatgac gcccaccttg acgaagatcc
     1561 tgccttgctc ctcgtgtctg ttcgccagga ggtagaaggc ccccaggccc gccatcacga
     1621 acgccccgca caccacggcc ccgcccatgt tgtgggcgaa ctgcgcccac atccacgagt
     1681 tgagcaggac ggcccagtaa ttatcgagct ggatggtgcc gttctcgagc acgcggtagc
     1741 ctacggggtg ctgcatccag gcgttggttg tgatgatgaa gtagcccgaa gcccaggtgc
     1801 cgacgaagac catgagcgaa gagaaccagt ggaccctctg tccgaacctg cgctcgccga
     1861 agaggaacag gcccacgaac gcggactcga ggaagaaggc gaaggctccc tccatcgcca
     1921 gcgtctgggc gattatgtcc ccggtggccc tggagaaggc tgcccagttg gtcccgaact
     1981 ggaactcgag cgggatgcca gtgacgaccc ccataacgaa ggtgatggcg aagatcttgc
     2041 cccaaaaccg cgccgcgacg ttgtatagct cgtcgcggtt gcgcatgtag acgctcttca
     2101 acacgaagat gagcagggca agccccatgg tgaacatcgg gaagaggtaa tggtatgtca
     2161 ccgtgaacgc gaactgtagc cgggataccg tcagtgcgtc ttccaaggcc gacctccagc
     2221 tgctctcaca tcaaagtcct caccctacaa gctcgtcagc gcctgcgcct gatcaatttg
     2281 aaccaatctc ggctccatga taaccatttc cacctcacga cgcggcgagc atcccgcgct
     2341 gcacgtcgag catcctgcgg tagagcccct ccgcccgcac cagctcttca tgggtgccgc
     2401 gctcgatgat gcggcccttc tcgaggacca ggatctcgtc catcgtctcc aacgtgacaa
     2461 gccggtgagt gaggatgagc gtgctccggt cgcgcatgag gtcctttatc tccgccatga
     2521 gctcgcgctc ggtgatggtg tccaggttgg cgctggcctc gtccagaacg agcaacggag
     2581 catccttgag caaaaccctg gcgatggcca gtcgctgccg ctcgccgccc gagagcgcga
     2641 ggccctgctc accgacgtag ctatccaacc cccgcggcag ccgctccacc agctcttcca
     2701 gccgggcccg cgcgagggcc ccgtgcagcg cgacgtccgt agcttgcggg tcggcgatca
     2761 acaagttggc gcgcagggtg tcgttgaaga catgggtgtt ctgggagacg aaaccgatcc
     2821 gggcgcgcaa ctcctcctgg acgtaggagc ggacgtcgcg gccgccgagc cggatctcac
     2881 cgctcacagg gtcccagaac cgaagcgcca ggctcaccag cgtgctcttg cctgacccgc
     2941 tcggtcccac gatagcgacc cggcacccag gtctcaaggc gaagctcacg tcctccagag
     3001 ccggaggcag gccctctcca tagcagaagc tcacccgatc gaactccaga gtaacgtcgc
     3061 agggcgcctt gagtggctca ggcgggtcga caacctccgg ctcggcgtct atcacctcga
     3121 agagccgctc cccggcgtgg acggagcggc ccagtgatcc gagagcgctt ccgagaggga
     3181 ccacggcctc gaagctgcca accattacca gcgcgaggaa cgccaggtac acgccgcgca
     3241 cctcccccgc cgcgacgagc ggcgccgcga gaaccaggat cgccacgagc gccaggccag
     3301 ccatcaggtc ggcgagggcg ctttgcagcc ccgtgacgag cgctgtcctc ccctgcgccc
     3361 ggtcgagcat acctgcgagg gtggagagct cgcgccgctc cctctcctcg gcgccgaagg
     3421 ccaggaggtc ttgaatcccg cgaacgtcgt cgacaacgcg cgcgcccaac tcggcccgca
     3481 gctccagttg ccggcgtccg agaccacgag agagcagccg caccagcagc ggcgcgccga
     3541 cgcctgtgac gacgaggaat cccgcggcag ccagggccat agcagtaccg aaagcgtgga
     3601 agacgaggaa ggtgatcagg gacaccacga tcgccacgac gagcggcgag acgacccgaa
     3661 ggtagacgtt ctccagctcc tcgacgtcct ttacgacgcg ggagagaagg tctccgctcc
     3721 tgtagcgggc caggcgggcg ggagcgagcg gctccagccg ggcgtagaac caggtgcgca
     3781 ggttccccag gagcttgaaa gtcacgtcgt gtgaggtcag cctctcggcg tagcgcgagg
     3841 ccgcgcgaga gacgctgaag aggcgcacca ggtagacggg gatcacgagc atcgagaggt
     3901 atggcacgat ggcggcggcg gagatcaagt acgcagccgt cgcgaggagc ccaacgttgc
     3961 taaccacggt ggccacgccc agcaggatgg cgagcgccac ctgccaacta tacggctgga
     4021 ggaagccgag caggcgcagg aacatcctca cgccggcacg tccccgtagg cgccagcgag
     4081 ccgggagtag gtcccgccac gggcgagcag ctctgcgtgg gtgcccacgt cctccaggcg
     4141 gccttcgtcg aggacggcga tccggtccgc ggcgagggcg gtgttgagcc ggtgggcgac
     4201 gacgagcgtc gtgcgaccac gcgccagacg ctcgatcgcg gccccgatta gcgcctcgct
     4261 ctcagggtcc aggctcgagg tcggctcgtc gaggaccagc agcggagcat ccttcaggaa
     4321 ggcgcgcgct atagccaccc tctgggcttc cccgccggag aggagcaggc cccgctcccc
     4381 gacctgggct tcgtaggcgc gggggaggtg gcggatgaag tccgcggccc ccgccagctc
     4441 cgccgcctcc tctacctcag cctggctcgc gccgggacgt gccagccgga tgttctcgag
     4501 caggctcccg tagaacaggt aaggccgctg cgggacca
//
LOCUS       DASTUV010000359         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_227630, whole genome shotgun sequence.
ACCESSION   DASTUV010000359 DASTUV010000000
VERSION     DASTUV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227630"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFT03_07180"
     CDS             complement(<1..1005)
                     /locus_tag="VFT03_07180"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4847965.1"
                     /translation="MNLYSRLRDRAAEGRPIRVGVIGAGKFGSMFLAQARTTPGIHVM
                     AVADLSAPRALQSLRNTGWVEEQISATSFTQALSSGTTHVTEDVEAMIAADGVDVIVE
                     ATGNPGAGIHHALRCFYHGRHLVMVNVEADALVGPLLARRAREAGMVYSLAYGDQPAL
                     ICEMVDWARATGFEVVCAGKGTKYLPEYHASTPDTVWEHYGFTDEQVEAGGLNPRMFN
                     SFIDGTKSAIEMAAVANATGLTPSPEGLGFPPAGADDLAAVCRPREDGGSLHHPGTVE
                     VVSSLERDGRTVERDLRWGVYVTFKAPTDYAARCFSEYGLVTDDSGFYTALYRPYHLI
                     G"
     gene            complement(1419..2924)
                     /locus_tag="VFT03_07185"
     CDS             complement(1419..2924)
                     /locus_tag="VFT03_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU4847966.1"
                     /translation="MDRSGESGTGPEGVGTASTPTQEYAEMAPSRNVPPMPYRDMPEP
                     LSLGKMMGPSVILAGLGVGSGEYILWPFMASNVGLAFMWAAVVGVTLQYFLNMEIERY
                     TLATGETAVVGFARFWKPWGILMCIGAVVPNMFPGWITSGVTIFGYLTGYGEGAVPYI
                     SIIALIALGIALTTSPVVYNTMEKAEFFKVGLTFVFLVIAIFSAITAEAVAKLFQGFA
                     NFGQVPIGVSEAVPIAVFLSGLIFAGAGGANNLVQSNWIRDKGFGMGAYIPRIVSPIT
                     GEEVAAPATGSMIRQDETNISRFRRWWRLANIEQFSTFWFICVFSILTFSMVAYSTVY
                     GKGIAEAADLTFIQAEGEQLANIVGGWFGALFWIFGTLSLVLVALGILDYVSRLVADV
                     LKTLYLRENDRWSESRMYFILVWSLVAIGTIILLAGFDAPLVLLVTSAVISGGVMAVY
                     SVLLIILNRRALPEAIKLKGWRLPVLVIAALFYIFFVGWFIVTQIQGLMGG"
BASE COUNT          648 a         1120 c         1054 g          580 t
ORIGIN      
        1 cccgatcagg tggtacggcc ggtagagcgc cgtgtagaac ccggagtcgt cggtcacgag
       61 cccgtactcc gagaagcagc gcgccgcgta gtccgtcggc gccttgaacg tcacgtacac
      121 cccccagcgc aggtcgcgtt ccacggtgcg gccgtcgcgc tcgaggctgg agacgacctc
      181 tactgtcccc gggtggtgga ggcttccgcc gtcctcgcgg ggccggcaga ccgcagccag
      241 gtcatcggcc ccggcgggcg ggaagccaag gccttcgggg gaaggagtga ggccggtggc
      301 gttggcgacc gcggccatct cgatggccga cttggttccg tcgatgaaag agttgaacat
      361 ccgcgggttg agcccaccgg cctccacctg ctcgtcggtg aacccgtagt gctcccacac
      421 ggtgtccggc gtggaggcgt ggtactcagg caggtacttc gtccccttgc cagcgcagac
      481 gacctcgaag cccgtcgccc tcgcccagtc caccatctcg catatgaggg cgggctggtc
      541 gccgtaggcc agcgagtaca ccattcctgc ctccctcgcg cgccgggcga ggagaggccc
      601 caccagggca tcggcctcga cgttgaccat gacgaggtgt cggccatgat agaaacaccg
      661 aagcgcgtga tggatgcccg ctcccggatt accggtcgcc tccacgatca cgtcgacgcc
      721 atcagcggcg atcatcgctt cgacgtcttc cgtgacgtgt gtggtgcctg agcttagggc
      781 ttgcgtgaac gaggtggccg agatctgctc ctctacccaa cccgtgttcc gtaacgattg
      841 cagagctcgt ggagcggaca gatccgcaac cgccatcacg tgaatgccag gagtcgttcg
      901 ggcttgcgcg aggaacatcg agccaaactt cccggctcct atgacaccga ccctgatcgg
      961 ccgcccctcg gccgcccgat cgcgcaagcg agagtacagg ttcacagctt tccaccacct
     1021 tacgacgcgt tcaacaatcc cctaattaca atgctagcat gaagcttcag tgcctgccgg
     1081 gcctaccagg ctcgcttatg ccagatgata gtcaaccggc aattcaagcc cctgctgcgg
     1141 cgtgtggagt tgccggagac acgctagccc gacctgcggt atctctgctt catgattctg
     1201 cagccgaggg gagagctcgt catggccttc gtggttctca ccaatgctaa gggcgtcttc
     1261 ggggagctcc ccgaagacgc ccttagtagc ctccagaacc gctcgggttg ctcccctcac
     1321 tcggggatga tcccgaagag ttcggccagg gccaacagga ccgcgaagac gacgatgaac
     1381 accatcaggc ggacgagcag gcgacgggcc acgtcccgct agccccccat caggccctgg
     1441 atctgcgtga cgatgaacca gcccacgaag aaaatgtaga agagcgccgc tatcaccaac
     1501 acgggcagcc gccagccttt gagcttgatc gcctcgggca gggccctgcg gttgagtatg
     1561 atgagcagga ccgagtagac ggccatgacg ccgccgctga tgaccgccga ggtcacgagc
     1621 aggaccaacg gcgcgtcgaa gcccgccagg aggatgatcg tcccgatggc gaccagcgac
     1681 cacactaata tgaagtacat ccggctctcg ctccagcggt cgttctccct caggtagagg
     1741 gtcttgagca cgtcggccac aagccgggag acgtagtcga ggatgcccag ggccacgagg
     1801 accagcgaga gggtgccgaa gatccagaag agggccccga accagccgcc gacgatgtta
     1861 gcgagctgct cgccctcggc ctggatgaag gtgaggtcgg ccgcctccgc gatgcccttg
     1921 ccgtacaccg tggagtaggc gaccatcgag aaggtgagga tggagaagac acagatgaac
     1981 cagaaggtgg agaactgctc tatgttggcg agcctccacc acctccggaa ccggctgatg
     2041 ttcgtctcat cctggcgtat catcgagccg gtcgcgggcg ccgccacctc ctcgccggtg
     2101 atgggcgaga ctatcctcgg gatgtaggcc cccatgccga agcccttgtc gcggatccag
     2161 ttgctctgca cgaggttgtt ggccccgccg gcgccggcga agatgagccc gctcaagaag
     2221 accgcgatcg ggaccgcctc ggacacacct atgggaacct ggccgaagtt cgcgaacccc
     2281 tggaagagct tcgccacggc ctccgccgtg atggcggaga agatagcgat gaccaggaac
     2341 acgaacgtca ggccgacctt gaagaactcg gccttctcca tggtgttgta gaccaccggc
     2401 gaggtcgtga gcgctatgcc cagggcgatg agcgcgatga tggagatgta ggggacggcc
     2461 ccctctccgt agccggtcag gtagccgaag atcgtcaccc cgctggtgat ccagcccggg
     2521 aacatgttcg gcaccaccgc cccgatgcac atcaggatgc cccagggctt ccagaaccgg
     2581 gcgaagccga cgactgcggt ctcgcccgtg gctaaagtgt agcgctcgat ctccatgttg
     2641 aggaagtact gtagggtcac cccgaccacg gcggcccaca tgaacgcgag cccgacgttc
     2701 gaggccatga agggccagag gatgtactcg cccgagccta ccccgaggcc cgcgaggatc
     2761 acgctaggac ccatcatctt gcccaacgac aacggctccg gcatgtcgcg gtagggcatg
     2821 ggtggcacgt tgcggctcgg ggccatctcg gcgtactctt gggttggcgt gctggcggtc
     2881 ccgaccccct cggggccagt cccactctct ccgctccgat ccacgacttt ttctcctttc
     2941 cgcgccgtac ggggttcgaa tcttctgcct tacaaaatca ttctcttcct cgggacaaca
     3001 cctcccctca cggcaggaat cccgttgcgg aagcgtagac gaagaggccc cggatagggg
     3061 cggaccccgt caactcggag ctttcccacc gaccccgacc ggacgaggag cctcaggcaa
     3121 gcggcgggca cccgtgggtg caccggaata gccgcgccag aaatgaggct cgacttactc
     3181 gttgctttcc ccttatgtat ccccaacccc tcaatggtgc gttaacaccc gtgcatcatt
     3241 gtacgttctg gcattatcca ccatcgttgc gccacggtcc acagaccgga gttacgggtt
     3301 ctgggcaagc ctagagcgtg cgagccgcct ctctggtagc acggcagttt tcccgctacg
     3361 gactatccag gcggccttcg acatggtgac cgtggcgacg ga
//
LOCUS       DASTUV010000360         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_502256, whole genome shotgun sequence.
ACCESSION   DASTUV010000360 DASTUV010000000
VERSION     DASTUV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502256"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..499)
                     /locus_tag="VFT03_07190"
     CDS             complement(5..499)
                     /locus_tag="VFT03_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847967.1"
                     /translation="MRKVMLLAAVMAMVLAAAAPAFAQATAVGGNVQYADCDQVQAAA
                     TGQYNTDNQQNAAGIAQELGVSQNQVLACLDSIAAGRDVNINASASASATSTATAKMK
                     AAKATSTATAGGGGGGGGGSASAASAAAGGALPSTGGASLLALGAGALLVAGGLLARR
                     IVRQ"
     gene            complement(793..1770)
                     /locus_tag="VFT03_07195"
     CDS             complement(793..1770)
                     /locus_tag="VFT03_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4847968.1"
                     /translation="MAFLSFIGPLGLVGLLFLLSPVEEERVVRAKLPTVKSRLAAPEL
                     DPELVAGNLRAELEQVADRYPASYGVVVFHPSSGETVVMNPNRSFVAGSLNKLPVLLT
                     LYRAAARGEVYLDGEITMLASDVQAYGTGVLYRRPVGTTMTLRECAELMITESDNTAW
                     VMLNRYLGRYNIEAELYSIGALSTAYLVPNTTTASDVLVMLEKVADPSYTSPELSAEM
                     LDIMTNTSFEDRLPQPLPEGARVAHKIGSYGDTFSDAGLVFTEGSQDAYYIVVIAGDT
                     TEGTARSAIQSMSLAAYESLASLAQQSRGNRGGSGTTAGGDTEHEGISR"
     gene            complement(2452..3204)
                     /locus_tag="VFT03_07200"
     CDS             complement(2452..3204)
                     /locus_tag="VFT03_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847969.1"
                     /translation="MEERRIPETLEEAYRLRDAASSLPEKDFYQEQVYRLRREEYRSR
                     GIRTEISSRRRERQSAIEFSREMFFLEVAEIAGHSSGRRGGRGARAAQQEARRYATRL
                     DTHAFLKQLASPHSGAVELYRRNYLGLLRMGKSPAEAYYLSRVIGLANEPEFRRALES
                     VRETGYPTKGRMAYTVEKAAIQIRDLPGGMILILLTFFASLFAFVWASKLWGPPASAG
                     TIFFVSLGLIAAGGLMLISAVFIVTFIRSINK"
     gene            complement(3294..>3570)
                     /locus_tag="VFT03_07205"
     CDS             complement(3294..>3570)
                     /locus_tag="VFT03_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847970.1"
                     /translation="GIAGLSEGEGRAEIPTGNSKERELLGALREYGELSPAQAAMETS
                     LSVTEADRMLKELAEGGHLDVRVRGGGLFYSLWERDEGEDIGRLEGR"
BASE COUNT          675 a         1127 c         1079 g          689 t
ORIGIN      
        1 ttagttactg ccgaacgatc ctgcgggcca gaagaccacc ggccaccagc agggcaccag
       61 cgccaagcgc cagcagcgat gcaccacccg tgctcgggag cgcgccgcca gcagccgccg
      121 aagccgccga agcggagccg ccgccgccgc cgccaccgcc accagccgta gcggtcgaag
      181 tcgccttagc cgccttcatc ttagcggtcg cagtcgacgt agccgaagcc gaagccgaag
      241 cgttgatgtt cacgtcacga ccagcagcga tactgtcgag gcacgcgagc acctggttct
      301 ggctgacgcc aagctcctgc gcgatgccgg ctgcgttctg ctggttatca gtgttgtact
      361 gacccgtagc cgcagcctgg acctggtcac agtccgcgta ctggacgttg ccaccgacgg
      421 ccgtcgcctg cgcgaaagcc ggggcggcgg cagccagtac catggccatg accgctgcta
      481 acaacattac cttcctcaac tcattacctc ctatgcctga ggattacctt acgtgccgca
      541 ttttttcatc cttaactcat aatgtcaaca ccattcaccg tattttttac aattttctta
      601 aaacttctta acaatatagt cgcctgtggg gagtttatcg tggacgggtt ccgagatcta
      661 gggtccacgc ggtcgttctg ggtctgttga ggtgatgtcc ggtagctgcg ctcacgccga
      721 catcatcgag cctgcggcgg aggatgttgg atccagccac gaagttagag ctgagcacca
      781 cggtcgcgtg tctcaacgag atatcccctc gtgctctgta tccccgccgg cggtggttcc
      841 cgagccccct ctatttccgc gggactgctg agcgaggctc gccagggatt cgtaggcggc
      901 cagggacatg ctctggatgg cggagcgcgc ggtaccctcg gtagtgtcgc cagcgatcac
      961 gactatatag taggcgtctt gggagccttc ggtgaagact agcccggcgt cgctgaaggt
     1021 gtcgccgtag gagcctattt tgtgggcgac gcgggctcct tcgggaagcg gctggggcag
     1081 gcggtcctcg aaggaggtgt tcgtcatgat gtcgagcatc tcggccgaga gctcggggct
     1141 cgtgtatgag gggtccgcga ctttctccag catcacgagg acgtcgctcg cggtagtggt
     1201 gttgggcacc aaatacgccg tcgagagagc gccgatactg tagagttcgg cctcgatgtt
     1261 gtacctgccc aggtagcggt tgagcatgac ccaggcggtg ttgtcgctct ccgtgatcat
     1321 cagctcggcg cactcgcgca gggtcatggt ggtgccgacg ggccggcggt acaggacgcc
     1381 cgtgccgtag gcctggacgt cggaggcgag catggtgatc tcgccgtcga gatacacctc
     1441 gccgcgggcg gcggccctgt agagcgtgag caggacgggc aacttgttaa ggctgcctgc
     1501 gacgaagctc cggttcgggt tcatcacgac ggtctcgccg gatgaggggt ggaagacgac
     1561 aaccccgtag gacgcggggt accggtccgc cacctgctcg agctcggccc tcaggttccc
     1621 ggccaccaac tccgggtcaa gctcgggcgc ggcgagccgg gactttaccg tagggagctt
     1681 cgcgcggacg acacgctcct cttctaccgg cgataggagg aaaagaagcc ccacgaggcc
     1741 caggggaccg atgaagctca gaaacgccac ccagcgcagc ccgcgccggt ggccgaagct
     1801 ccagggtttc ctcagttgct ggcgccgttc ccggcggctc agaagcgttt cgggaggggt
     1861 ctgcggttta ggccccggtg gatgtatggg tccccctgta gcccgggttc gcgccccggc
     1921 acgccggggc tacttactag tggacccgat aaaggtaact acaaagacgg cgagggtcaa
     1981 tacctctacc tgagagttaa tcgccaactc tcctaaacta cttgacagtt tggggtgaca
     2041 gtcgagagga caagaggcac ctacccgcgc tgggggcaag gtcccgtacg tccacataag
     2101 ccctcttctg ctatccccac ctactatatc ttgtgccgga taacattctg attgcaacaa
     2161 cctatacaac tttgtgcgag aagtgtaaga taaaaatagt tagccggtac ccatatcctc
     2221 ctttcccatt tacacgatac gggtaccggc tctcaaaaag catacaaagc atagcgcctt
     2281 tctaatgccc cgcgcaaccc tttgttacgt agtctttacg acggataatc gaccttcatg
     2341 ccatggcgtc gcctccgctt acaattatcg acaggttaga gtgcgggagt tgacaggagc
     2401 gaggcaggga aatcgggcta agaggcgcgc tcaggcgcgg gtacgccggg gctacttgtt
     2461 aatagacctt ataaaggtaa ctataaagac ggctgagatc aacataagtc cccctgcggc
     2521 gatcaggccc agagagacga agaagatggt gcccgcgctc gccggaggac cccacagctt
     2581 gctggcccac acgaaggcga agagcgaggc gaagaaggtc aggaggatca ggatcatccc
     2641 tccggggagg tcccggatct ggatcgccgc cttctcgacc gtgtaggcca tgcggccctt
     2701 ggtggggtaa cccgtctccc tcacgctctc gagtgcccgc cggaactcgg gttcattcgc
     2761 gagaccgatg acccgcgaga ggtagtaggc ctctgccggc gacttgccca tcctcagcag
     2821 accgaggtag ttgcgtctat atagctccac ggccccagag tgaggcgagg ctagctgctt
     2881 caaaaaggcg tgggtgtcga ggcgggtggc gtagcggcga gcctcctgct gggctgcgcg
     2941 ggctccgcgg ccgcccctgc gtccggagga gtggcccgct atctccgcga cctcgaggaa
     3001 gaacatctcg cgggagaact cgatggcgct ctggcgctcg cgcctgcgag aggagatctc
     3061 cgttctgatg ccgcgcgagc ggtactcctc gcgcctcagg cggtagacct gctcctggta
     3121 gaaatctttc tccgggaggg aggaggccgc gtcccgcagc ctgtaggcct cctcgagcgt
     3181 ctccgggatc cttcgctctt ccatcgctta agttcttacc ccacgctcac gatctcacgc
     3241 gccgcaaccc taggaccgtg aatgatatca cgagaacgat gttccatacc cctctaccgg
     3301 ccctccagac ggcctatatc ttcgccctca tcgcgctccc acagcgagta gaacaatccg
     3361 ccgccgcgca cccgcacgtc caggtgccct ccctcggcga gctccttgag catgcggtcg
     3421 gcctccgtaa ccgagagcga ggtctccatc gccgcctgag ccggactcaa ctctccatac
     3481 tcccgtaaag cccccagaag ctcccgttcc ttgctgtttc cggtcgggat ctctgcccta
     3541 ccttctccct cagagagccc cgcgataccc
//
LOCUS       DASTUV010000361         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1354641, whole genome shotgun sequence.
ACCESSION   DASTUV010000361 DASTUV010000000
VERSION     DASTUV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354641"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFT03_07210"
     CDS             complement(<1..787)
                     /locus_tag="VFT03_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU4847971.1"
                     /translation="MTTPKDGTQTTLRSLLDGTATYAAGFLDGLPERKVATTATAEEL
                     RKELGGPLPEGPHHPEMVVAELARAADPGIMGTPSGRFFGFVIGGSLPAALAADWLTS
                     AWDQNAGLYAAAPAASVVEEVCGAWLIELFGLPEHVSYGLVTGCQMANFTALAAARHS
                     VLEAAGWDVEADGLVGAPPIRIILGEERHVTIDRALRFLGLGTANLRTVPADGQGRMR
                     ADALEDALTESSGPTIVCAQAGNVNTGAVDPLRAICAAAREVGA"
     gene            complement(888..1700)
                     /locus_tag="VFT03_07215"
     CDS             complement(888..1700)
                     /locus_tag="VFT03_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU4847972.1"
                     /translation="MYLSRHFTARGARWALDGRYLPTAFTLDLLAESPAGDTPTFLGS
                     LPRGEAAEDPLLPPVEPAQEVWASGVTYERSREARMFESKEADIYDKVYDAVRPELFF
                     KAIGWRVAGHEGAVRVREDSNWNVPEPELVLVLNDRMEILGYTAGNDVSSRDIEGENS
                     LYLPQAKIYDGSCSLGPGIVLGGPDSMRDLLIRMRILRDGEAVYEDEVSTSRMKRPFE
                     ELADYLGMELSFPTGAFLMTGTSLVPGEDFTLTPGDRVEISVGDLVLENEVV"
     gene            1820..>2407
                     /locus_tag="VFT03_07220"
     CDS             1820..>2407
                     /locus_tag="VFT03_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4847973.1"
                     /translation="MEILPGIHRIESDLGVRFMCQYLLTGEDHTVLVDTGLAGTPEEV
                     IIPYLEGVGLSLEDIDEVIISHADVDHCGGNRTLKEMHPALWFTCGEPDRAYVENNQV
                     MIAEIYRWSEPYGFGPDEDSLEWIRRELGGDSPVDAGLRGGETMRLGPERRIEILHLP
                     GHTPGHLGIWDPKNRAAIIIDAALETGIYDREGNRL"
BASE COUNT          417 a          796 c          789 g          405 t
ORIGIN      
        1 aagcgccgac ttcccgggca gcggcgcaga tagcgcggag gggatcgaca gcgccggtgt
       61 tcacgttgcc cgcttgcgcg cacactatgg tcggtccaga ggactcggtg agagcatcct
      121 cgagcgcgtc tgcccgcatc ctgccttgac cgtcggccgg gaccgtccgc aggttggcgg
      181 tcccgagacc caggaagcgc aaagcgcggt ctatggtgac gtggcgctcc tcaccgagga
      241 taatccggat cggcggcgcg ccgaccaggc cgtcggcctc tacgtcccaa ccggccgcct
      301 cgagcacgct gtggcgcgcg gcggcgagcg cggtgaagtt cgccatctgg cagccggtca
      361 ccagcccgta tgagacatgc tcgggcagcc cgaacagctc gatcaaccaa gcgccgcaca
      421 cctcctcgac caccgaggcc gcgggggcag ccgcgtacag cccggcgttc tggtcccaag
      481 ccgaggtgag ccagtctgcg gcaagggcag cggggaggct accccctatg acgaagccga
      541 agaaccgacc gctcggcgtg cccatgatcc cgggatcagc cgccctcgcg agctccgcga
      601 cgaccatctc cggatgatgc gggccttcgg gcagcggtcc gcctaactcc ttgcgcaact
      661 cttcggcggt ggcggtagtc gcgaccttgc gctctgggag tccgtccaag aacccggccg
      721 cgtaagtcgc cgtgccgtcc aacaacgaac gcagagtggt ttgcgtaccg tctttagggg
      781 tcgtcatggc tcactcctct ctgcgctgtc ctgcgcatat cctcagagcc caggttgtcc
      841 ctctgcccca tcgctctcgg tactggctcg tatggagggt tgttctatca gacaacctcg
      901 ttttcgagca ccagatcgcc gacggagatc tcgacgcggt ccccgggcgt gagggtgaag
      961 tcttcgccgg gaacgaggct ggtacccgtc atcaggaagg cccccgtcgg gaatgagagc
     1021 tccataccga ggtaatcggc tagctcctcg aaggggcgct tcatccggga cgtgctcacc
     1081 tcgtcctcgt agacggcttc gccgtcgcgc aagatgcgca tccggatgag gaggtcccgc
     1141 atcgaatccg gcccgcccag aactataccg ggcccaagag agcagctgcc gtcgtagatc
     1201 ttggcttgcg gcagatagag agagttttca ccctctatat cccgcgacga gacgtcgttg
     1261 ccggcagtgt agcccaggat ctccatccgg tcgttgagca ccagcaccag ctcgggctcc
     1321 ggcacgttcc agttgctgtc ctcgcggacg cgcacggccc cttcgtgccc cgcgactcgc
     1381 cagccgatgg ccttgaagaa gagctcgggc cgcacggcat cgtagacctt gtcgtagatg
     1441 tcggcttcct tcgattcgaa cattcgagct tcccggctcc tctcgtacgt caccccgctc
     1501 gcccacacct cctgcgcggg ctcgacgggc ggtaggagag gatcttcggc cgcctcgccg
     1561 cggggcaggc ttccgagaaa agtaggggtg tcaccggcgg gcgattccgc caggagatcc
     1621 agggtaaacg ccgtgggcag gtagcgcccg tccagtgccc agcgagcgcc gcgtgccgtg
     1681 aagtgtctgg ataggtacat gtctgttctc gaacctcctt ccccgatccg gggtacaagc
     1741 taacacggct gccgcgtgct tgcgcgggaa ggcgggcacg gttagggtga gccgcgcaat
     1801 ctgaagagag ggggtgagag tggagatact gccgggcatc catcggatcg agtcggacct
     1861 gggggtacgc ttcatgtgcc agtacctcct aaccggggag gaccacacgg tcctcgtcga
     1921 taccggcctt gccgggacgc ccgaggaggt catcatcccc tacctggagg gggtcgggct
     1981 ctctctggag gacatcgacg aggtcatcat cagccacgcc gacgtggacc actgcggcgg
     2041 caaccgcacc ctcaaggaga tgcatcccgc tctgtggttc acgtgcggcg agccagacag
     2101 ggcctacgtc gagaacaacc aggtgatgat cgcggagatc taccgctggt ctgagcccta
     2161 cggcttcggc ccggacgaag actctctaga gtggatccgc agggagctgg gcggcgactc
     2221 ccccgtggac gccgggctgc gcggcgggga gactatgagg ctggggcccg agaggcgtat
     2281 cgagatactg cacctccctg gccacacgcc gggccacctc ggcatctggg acccgaagaa
     2341 cagggcggcg attatcattg acgcggcgct cgagaccggc atctacgacc gcgaggggaa
     2401 ccgtctg
//
LOCUS       DASTUV010000362         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1297137, whole genome shotgun sequence.
ACCESSION   DASTUV010000362 DASTUV010000000
VERSION     DASTUV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297137"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFT03_07225"
     CDS             <1..616
                     /locus_tag="VFT03_07225"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU4847974.1"
                     /translation="GYDTAVSIATEAIDRVRTTARSHERIIVVEVMGRHAGWITWGAG
                     LASAANVTLIPEVEPDLEAIATLFKQRAERGEKWGIVAVSEGVTFSEDFVTQTAEKDE
                     FGHVRLGGVGEGLAGELKERTGIDTRHVVLGHLQRGGTPTAFDRILSTRYGLRAAEAV
                     KNGEWGQMVALRGNDIVTVSLDEATAETKTVPDDLYRAAQTFFG"
     gene            complement(647..1609)
                     /locus_tag="VFT03_07230"
     CDS             complement(647..1609)
                     /locus_tag="VFT03_07230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU4847975.1"
                     /translation="MILEFERPIRDLEERIAELHRLAGPSAGLQAEISRLESTLDSAK
                     RRIYANLSPYQRVQVARHPDRPNFRAYRDELSDDFYELSGDRFFGDDPAVRGGFARLK
                     RGGHNVVLLGHDKGADTRSRVKGNFGMAHPEGYRKVRRLYELAGRFGLPIVVLVDTPG
                     AFAGRTGEERGQAWAISADLMALARAPVPVISMIIGEGGSGGALAMCVADYVGMLENS
                     YLSVIAPEACASIIFRDSSRAAEAAEALKLTARDLKLHGIVDEVLPEPLGGAHKEPKT
                     AIRDVGDALDRVISGLSAAEPDDLIEARHERYRRIGAHVGNEAT"
     gene            complement(1606..2451)
                     /gene="accD"
                     /locus_tag="VFT03_07235"
     CDS             complement(1606..2451)
                     /gene="accD"
                     /locus_tag="VFT03_07235"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU4847976.1"
                     /translation="MRNWLSKRREYTRSAPETESGIGPLDDTGFTKCERCGQPIYEKD
                     LLARFNVCPHCEFHYPLPAPERIRLLTDAGSFEERDRGMAAGDPLEFEGYEERLVHAR
                     KNTGLADAVLSGVGKIGGRTAALAVMDFRFIGGSMGSVVGERVARTIEHASVEGLPLV
                     VVSSSGGARMFEGVYSLMQLAKTSVALSRYMDGSLPYVSVLTDPTFGGVTASFATAAD
                     VIIAEPGARIGFAGARVIEQTTRERLPEGFQTAEFQRDHGMVDRIVHRLALKGDLERL
                     LGFLS"
     gene            complement(2435..3244)
                     /gene="trpA"
                     /locus_tag="VFT03_07240"
     CDS             complement(2435..3244)
                     /gene="trpA"
                     /locus_tag="VFT03_07240"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU4847977.1"
                     /translation="MTGSERLMTAFRDDRVALIPYLTGGYPTLEGAREVGEAYVAAGA
                     DVVEIGVPFSDPLADGPTIQDTTTLAIENGADLKYCLDLASTFSDRVPVVFLLYYNVI
                     FARGIEEFLREAAAAGVSGLVVPDLPVDEAGEFTRLSSEAGVAFCPLVAPTSTDERLA
                     RVGELASGFVYCVSVAGVTGVRDALPSGAVELLRRARAKTDAPVALGFGIGSPEAAVE
                     AAREADGVIIGSKLMRLVGEGGPERAGGWLREVSEALSGVVKSGGRDQELA"
     gene            complement(3234..>3895)
                     /locus_tag="VFT03_07245"
     CDS             complement(3234..>3895)
                     /locus_tag="VFT03_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU4847978.1"
                     /translation="DTHYIIGTVAGPAPYPRIVRDLQAIIGRELEVQARERFGGDPDA
                     VVACVGGGSNAIGAFYPFVGREGVRLVGVEAGGRGLETGEHGASLTGGRPGVLHGNLS
                     YVLQTEEGQIREAHSISAGLDYPSVGPEHAYLKDSGLAEYVSATDEEALEAFILCSRL
                     EGIIPALEPSHAIFYAEKVARELGPGKNLVINLSGRGDKDVEVVAEALGVTAEEVSGD
                     G"
BASE COUNT          650 a         1391 c         1247 g          607 t
ORIGIN      
        1 cggctacgac accgcagtct ccatagccac cgaggccata gatagagtgc ggaccaccgc
       61 caggagccac gagaggatca tcgtcgtcga ggtaatgggg cggcacgccg gttggatcac
      121 ctggggcgca ggcctcgcct ccgccgcgaa cgttactctc attcctgaag tggaaccgga
      181 tcttgaagcg atcgccaccc tcttcaagca gcgcgccgag cgaggcgaga agtggggcat
      241 cgtcgccgtg agcgagggag tgaccttcag cgaggacttc gtgacccaga cggcagagaa
      301 ggacgagttc ggccacgtcc gtctcggcgg cgtgggcgaa ggccttgccg gagaactcaa
      361 ggagcgcaca ggtatagaca cccgccacgt cgtcctcggc cacctgcagc gtggtggcac
      421 cccgacagcc ttcgaccgca tcctctcgac ccgctatgga ttgcgcgcgg cagaggccgt
      481 caagaacggc gagtggggcc agatggtcgc gctcaggggc aacgacatcg ttaccgtctc
      541 cctcgacgag gcaaccgcag agaccaagac cgtccccgac gacctttaca gggcagcaca
      601 gaccttcttc ggctagagat cgcgtccggt tcctctcgag gtgacgctag gtagcttcgt
      661 tcccgacatg cgccccgatg cggcggtagc gctcgtggcg ggcctcgata aggtcatcgg
      721 gctccgcggc ggagaggccg gagataacgc ggtccagcgc gtcgcccacg tcccggattg
      781 cggtcttggg ctccttgtgg gctccgccga ggggctcggg gaggacttcg tcaacgatac
      841 cgtgcagctt caggtccctt gccgtcagct tcagcgcctc ggcggcctct gcggcccggc
      901 tcgaatcgcg aaagatgatc gaagcgcaag cctcgggcgc tatgaccgag aggtatgagt
      961 tctcgagcat cccgacgtag tccgcgacgc acatcgccag agcgccgcca gagcctccct
     1021 cgccaatgat catggagatg accgggacag gcgctctggc gagcgccatg aggtcggcgg
     1081 agatcgccca ggcctggccc ctctcctcgc ccgtgcgccc ggcgaacgcg cccggcgtgt
     1141 ccaccagcac cacgatcggg aggccgaagc gcccggccag ctcgtagagc ctccttacct
     1201 tgcggtagcc ctccgggtgc gccatgccga agttgccctt caccctgctc ctcgtgtccg
     1261 cgcccttgtc gtggccgagg aggaccacgt tgtggccgcc gcgcttgagc ctggcgaagc
     1321 ccccgcgtac cgccggatcg tcgccgaaga accggtcgcc agagagctcg tagaagtcgt
     1381 ccgagagctc gtcgcggtag gcgcggaagt tgggacggtc ggggtggcgc gcgacctgca
     1441 cgcgctggta gggggagagg ttcgcgtaga tccgccgctt ggcggagtcg agcgtgcttt
     1501 cgaggcgtga gatctccgcc tgcaggcccg cgctcgggcc cgcgagccgg tgcagctcgg
     1561 cgatgcgctc ctcgaggtct ctgatcgggc gctcgaactc caggatcacg agaggaaccc
     1621 cagcaatctc tcgaggtcac ctttgagcgc gagcctgtgc acgattcgat cgaccatccc
     1681 gtgatcccgc tggaattctg ccgtctggaa gccctcgggc agcctctcgc gcgtcgtctg
     1741 ctcgatgacc cgcgcacccg cgaacccgat cctcgccccc ggctcggcga tgatcacgtc
     1801 cgcggccgtg gcgaacgaag ccgtcactcc cccaaacgta gggtctgtca ggaccgagac
     1861 gtaaggcaac gagccgtcca tgtacctcga gagcgcgacg ctcgtcttcg ctagctgcat
     1921 gagggaatag acgccttcga acatccgggc tccccccgac gacgagacga ccactagcgg
     1981 cagcccttcg acggaggcgt gctcgatggt ccgggcgacc cgctcgccga cgacgctccc
     2041 catagacccc cctataaacc ggaagtccat gaccgcgaga gccgccgtcc ggcccccgat
     2101 cttccctacc ccgctcaaca cggcatcggc gagacccgtg ttctttctcg cgtgcacgag
     2161 cctctcctcg tagccctcga actccagagg atcccctgcc gccatgcccc ggtcgcgctc
     2221 ctcgaagctc ccggcatcgg tgagaagccg gatccgctcc ggcgctggca gggggtagtg
     2281 gaactcgcag tggggacaaa cgttgaaccg ggcgaggagg tccttctcgt agatcggctg
     2341 cccgcaccgc tcgcacttgg taaagccggt atcgtcgagc gggccgatcc cgctctctgt
     2401 ctcgggggcg ctccgcgtat actcccgccg cttgctaagc cagttcctga tcgcgccccc
     2461 ccgacttcac gacgccggag agcgcctcgc tcacctcgcg cagccacccc ccggccctct
     2521 ccggtccacc ctccccgacc aggcgcatca gcttgctccc gatgatgacg ccgtcagcct
     2581 ccctggcggc ttcgacagcc gcctcgggcg agccgatacc gaaccccagc gcgacaggcg
     2641 catcggtctt cgcacgcgcc cgccgcaaaa gctcgacggc gccggagggc agggcgtcgc
     2701 gcacacccgt tacccctgcg accgagacgc agtagacgaa cccggaggca agctcaccca
     2761 cccgcgcgag ccgctcgtcc gtcgaggttg gcgccacgag cgggcagaag gctacgccgg
     2821 cttccgaaga gagccgcgtg aactctcctg cctcgtccac gggcaggtcg ggaacgacga
     2881 gccccgagac ccccgccgcc gcggcctcgc gcaggaactc ctcgataccg cgggcgaaga
     2941 tcacgttgta atagaggaga aagactaccg ggacgcggtc cgagaaggta gaggcgaggt
     3001 caaggcagta tttaaggtcc gcgccgttct cgatggccag ggtcgtggta tcctggatcg
     3061 tcggtccgtc cgccaggggg tcggagaagg ggaccccgat ctcgaccacg tccgcgccag
     3121 ccgcgacgta ggcctccccc acctcccgcg ccccttccag ggtgggatac ccgcccgtca
     3181 ggtacgggat gagcgcaacc cggtcgtctc gaaacgccgt catgagccgc tcgctacccg
     3241 tcaccggaga cctcttccgc cgtgacaccc agggcttcag ccaccacctc gacgtccttg
     3301 tcgccgcggc ccgagaggtt gatgaccagg ttcttgccgg gtccgagctc tctagccacc
     3361 ttctcggcgt agaagatcgc gtgcgagggt tcgagcgcgg gaatgatgcc ttcgagacgc
     3421 gagcagagga tgaaggcctc gagggcctcc tcgtcggtgg cgctcacgta ctcggccagt
     3481 cccgagtcct tgaggtaggc gtgctcggga cctactgaag ggtaatccag gcccgcggag
     3541 atcgagtgcg cctcccggat ctggccctct tcggtctgta ggacgtagct caggttgccg
     3601 tgtagcacgc cggggcgccc gccggtgagc gaggccccgt gctcccccgt ctcgagcccc
     3661 ctaccgccag cctccacgcc aaccaggcgc accccctcgc gcccgacgaa cgggtagaag
     3721 gccccgatgg cgttcgagcc gccccccacg caggcgacta cggcgtctgg gtcccccccg
     3781 aaccgctcgc gcgcctgcac ctccagctcc ctaccgatga tggcctggag gtcccgcacg
     3841 atacgcgggt agggcgcggg tccagccacg gttccgatga tgtagtgggt gtcct
//
LOCUS       DASTUV010000363         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1238471, whole genome shotgun sequence.
ACCESSION   DASTUV010000363 DASTUV010000000
VERSION     DASTUV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238471"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VFT03_07250"
     CDS             <1..1132
                     /locus_tag="VFT03_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU4847979.1"
                     /translation="AIDEPVETHYYDTAAIMYTSGTTGPSKGVVVPYGVEGIFAQGVV
                     DTAKLTSDDVNYVVHPLFHANAQFMQILPSLLVGARISLWPGFSASRWLDQVRATGAT
                     ITNTLGVMCNFIHSQPVREDDADNPLRAMITLPAPSDIAEDIERRFGVKCLEGYGMTE
                     IGVVTYRRLEEPLRLGSAGRALEWFDVRVVDPETDEPLPPGGVGEIVVRPKATGIMMK
                     EYNNVPAKTVEAWRNLWFHTGDAGEMDEEGYLYFRDRVKDVIRRRGENISSFSIESVA
                     NTHPTVEESAAIAVPAEHGVGAEDEVKLCVVLEHGEELDPAELHAYCAQRMPYFAVPR
                     YIEYVAQIPKTANEKVRKNVLREQGITPDTWDREAAGIRVRK"
     gene            1211..2881
                     /locus_tag="VFT03_07255"
     CDS             1211..2881
                     /locus_tag="VFT03_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU4847980.1"
                     /translation="MLNASDFWEALASRMPRQLDYLSLCEWFAWDLPSNYNIGVDVCD
                     RHAADKGKLAIIHDRGDGTAEKWTFWELKRASDRFANALRTLGVERGDRVAVLLSQSP
                     QLPIAHIATYKLGAVVVPLFTLFGEEALRFRLGDSGAEVIVTDLKHFEVVSTLREELD
                     GLQHVILVDGESSGARTFDGLIQDASSVFEPVATRPEDPAIIIYTSGTTGAPKGALHG
                     HGILLGHLPGVSLPHDLAPREGDLFWTPADWAWIGGLFDVLFPALHWGLPVVSHRMLR
                     FDPERSLALMERWGVRNAFFPPTALKMMRTVPDPERWKLELQTIGCGGEPLGVETFDW
                     AKEELGIVINEFYGQTECNLVLANCNAIMPAKPGSMGRPVPGHRVAVIDEEGNELGPG
                     EQGEVAVRSPDPAMMLGYWNNEAATKDKFVGDWLKTGDLASIDDQGYFHFVGRDDDVI
                     VSAGYRIGPVEIEETLVKHRDVLMAAAVGKADPVRGQVVKAFVVLREESEAGEDLAAE
                     LKGLVKSRLGAHEYPREIEFVDELPMTPTGKIRRNVLRQSEAGEGAKR"
     gene            complement(3045..>3744)
                     /locus_tag="VFT03_07260"
     CDS             complement(3045..>3744)
                     /locus_tag="VFT03_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4847981.1"
                     /translation="AGAAGSLIQRLPRARLFVHRVGAPHMIDPSKLLASATRIFQEDM
                     EQLWGEILPVPEDRVVILDDESTLRIGGRVLKALYTPGHASHHLAYYDAESGVIFTGD
                     VAGARLSEAEYLRPPTLPPEVDLTLWRQSITRLRSLQARRLYLTHGGAFSDPDRHFDL
                     LLSRLFFLAGWTEASLAVEQDTAVLASKLLQRESKEIIAITGNDDLVEHYELVAGSRM
                     SVDGLARHIQKRYR"
BASE COUNT          760 a         1085 c         1209 g          690 t
ORIGIN      
        1 ggctatagac gagcccgtcg agacccacta ctatgatact gcggccatca tgtacacctc
       61 cggaacgacg ggtccttcga aaggcgtcgt ggtgccttac ggggtcgaag gaatcttcgc
      121 tcagggagtc gtcgatacag cgaaactgac tagcgacgat gtgaattatg tggttcatcc
      181 gctgttccat gcgaacgcgc agttcatgca gattctcccg tcgttgctcg tcggagcgcg
      241 catcagcctt tggccgggct ttagcgcttc ccgatggctc gaccaggtgc gggcaactgg
      301 agcaacgata accaacacct taggagtgat gtgcaacttc atacatagtc agcccgtacg
      361 cgaggacgac gctgacaatc cacttcgcgc gatgatcacc ctgccggcac caagcgatat
      421 cgccgaagac atagagaggc gatttggggt caagtgcctg gagggctacg gcatgacgga
      481 aataggtgtc gttacgtatc gacgcctgga ggagcctttg cgtttgggtt cggccggtag
      541 ggctttggag tggttcgacg tgagagtcgt ggaccccgaa acggatgaac cgctgcctcc
      601 gggtggggtg ggcgagatcg tagtgcgccc gaaggctacg gggatcatga tgaaggaata
      661 caataacgtt cctgcgaaaa ccgtagaagc ttggcgcaat ctctggttcc atacagggga
      721 tgcgggtgag atggacgaag aaggctacct ctacttccgc gatcgcgtaa aagacgtcat
      781 ccggcgccgc ggtgagaaca tctcgtcgtt ttctatcgag agcgtggcaa acactcaccc
      841 aaccgttgag gagtctgcag cgattgccgt cccagccgaa cacggcgtgg gggcagagga
      901 cgaggtcaag ctttgcgtcg tactcgagca cggagaagag ctggacccag cggaactcca
      961 cgcgtattgc gcgcagagga tgccttattt tgcagtcccg cgctacatcg agtacgttgc
     1021 acaaataccc aaaacggcca atgagaaggt ccggaagaac gtcctcaggg aacaaggaat
     1081 cacccccgat acgtgggatc gtgaagcggc gggcattcga gtgcgaaagt aaagtgtccc
     1141 gcgttgctga acttcccagt atgtaacgtt ttgacacgga gcctcgaagt ctggtaaggg
     1201 ggaaagaaga gtgctaaacg cttcagattt ctgggaggcg ctggcttccc ggatgcccag
     1261 gcagctcgac tacctctcgc tctgcgagtg gttcgcctgg gatctgccct cgaactacaa
     1321 catcggggtg gacgtctgcg atcgccacgc cgcagacaag ggcaagctcg ccatcatcca
     1381 cgaccgtggg gacggcacgg cggagaagtg gaccttctgg gagctcaaac gggcctcgga
     1441 ccgcttcgcc aacgccttgc gaacgctagg tgtcgagcgc ggggaccggg tggccgtgct
     1501 gctctcgcag tcgcctcagc tccccatagc ccacatcgcc acgtacaagc tgggcgccgt
     1561 cgtcgtccct ctcttcacgc tcttcgggga ggaggccctg cgtttccggc tgggagacag
     1621 cggggcggag gtgatcgtga ccgacctcaa gcacttcgag gtcgtctcga cgttgcgcga
     1681 ggagctggac gggctgcagc acgttatcct ggtcgatggt gagagctccg gggcgcgtac
     1741 tttcgacggc ttgatccagg acgcctcgag cgtcttcgag ccggtcgcca cccggcccga
     1801 ggatccggcg atcatcatct acacttcggg gacgactggt gcgcctaagg gcgccctgca
     1861 cggccacggg atactgctcg gacatctacc aggtgtcagc ctaccgcacg acctggcacc
     1921 ccgcgagggg gacctcttct ggaccccggc ggactgggcc tggatcggtg gtctcttcga
     1981 cgtcctcttc ccggcgttgc actggggtct gccagttgtc tcccaccgga tgctccggtt
     2041 cgacccggag cggtccctcg ccctgatgga gcgatggggg gtccgcaacg cgttcttccc
     2101 accgacagcg ctcaagatga tgcgcaccgt ccccgatcca gagcggtgga agctggagct
     2161 tcaaaccatc ggatgcggcg gggagccctt gggggtagag acgttcgact gggcgaagga
     2221 ggagctcggg atcgtcatca acgagttcta cggccagacc gagtgcaacc tggttctcgc
     2281 caactgcaac gcgatcatgc ccgccaaacc aggttcgatg ggccgtccgg tcccaggcca
     2341 ccgcgtcgcg gtcatagacg aggaggggaa cgagctcggt cccggcgagc agggcgaggt
     2401 cgccgtgcga agtccagacc cggcgatgat gctcggctac tggaataacg aggcggccac
     2461 gaaagacaag ttcgtcggag attggctcaa gaccggtgac ctcgcctcga ttgatgacca
     2521 ggggtacttc cacttcgtcg ggagggatga cgatgtgatc gtctccgccg gctaccgcat
     2581 cggtccggtg gagatcgagg agaccctagt caagcaccgc gacgtcctga tggcggccgc
     2641 ggtcggaaag gcggatcccg tgcggggcca ggtggtcaag gccttcgtcg tcctgcgaga
     2701 ggaaagtgaa gcgggcgagg atctcgcggc ggagctcaag gggctagtca agagtcgcct
     2761 gggcgcgcac gagtacccgc gtgagatcga gttcgtggac gagctgccga tgacccccac
     2821 gggcaagatc cggaggaacg tcctgcgcca gagcgaagcc ggagaaggag caaagcgatg
     2881 agtatgttga tggcgccaac aaaggtgctg tatacgaagc atcctgagga ggtgaggacg
     2941 cactactcga ggtcctccgc gcgcttgaac taatttgttg aagatccccg aggcggccac
     3001 cactgcaagt gccgatgaca ggctactgat ccagggataa ccgtctacct gtaccgcttt
     3061 tgtatgtggc gtgccagacc gtcaacgctc atccgggaac cggcgacaag ctcataatgc
     3121 tcgactagat cgtcattacc agtaatggcg atgatctcct tactttccct ctgaagtaac
     3181 ttgcttgcga gaaccgcagt atcctgctcc actgccaagc tcgcctcggt ccagccggcc
     3241 aagaagaaca gccgggatag tagaagatcg aagtgtcgat ccgggtcact aaaggctccg
     3301 ccgtgggtaa ggtagagccg acgtgcctgt agactcctca ggcgagtgat gctctggcgc
     3361 cagagggtca ggtcgacctc aggcggtagc gtcggcggcc gcagatattc cgcctcgctg
     3421 aggcgtgctc cagcgacgtc gcctgtgaag attacaccgc tctcggcatc gtagtaggcg
     3481 agatgatgcg aggcgtgccc gggtgtgtag agggctttaa ggacgcgtcc gccgatccta
     3541 agcgtggact cgtcgtccag gataacaacc cgatcctccg gcactggcag tatctcgccc
     3601 cagagctgct ccatgtcctc ctggaaaatt cgcgtggcgc ttgccagcag cttagaaggg
     3661 tcgatcatgt gcggggcgcc aactcggtgg acgaacaagc gcgcccgcgg aagtcgctgg
     3721 atcagcgagc ccgcggctcc ggcg
//
LOCUS       DASTUV010000364         6894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_48771, whole genome shotgun sequence.
ACCESSION   DASTUV010000364 DASTUV010000000
VERSION     DASTUV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48771"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFT03_07265"
     CDS             <1..257
                     /locus_tag="VFT03_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEU4847982.1"
                     /translation="ALDKALRQAIEPHYPELKEIHLSNYKVRILDEHRATAATTRVLI
                     DSTDGKRVWGAVGVGENIIEASWQALVDGLEYGVNRTGKD"
     gene            complement(484..1554)
                     /locus_tag="VFT03_07270"
     CDS             complement(484..1554)
                     /locus_tag="VFT03_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4847983.1"
                     /translation="MSGGGSDAPLEIGRTLELARKRRGLSLKQVEEATKIRAVYLADL
                     EWEHFDVLPAVYMQGYLKTYASFLHLDADAMVGELKRRQSLSEEPPYPLYVGPSEDDS
                     LDDILTALGGAAKSRDATRDEEETVPTPRPAGFDARLYLGSAVFLLLCAAVVALALTV
                     AGDERPSAVSQVREPLTSRAPEASPAGAKEHASVQNPQQDDEQKGTNEADKQRSTADE
                     KGTSHSDRSASAAEDKAADPMPQNSASAQATASPAATASAASAREDRKPATTEPSRRN
                     AASIEAPTRQDDTASPSSPSPSSPSPSSPSPSSPPPTREPGGAPSGGNSSPPPRNDGV
                     VNVNVNVGGKDPVQLSGGPFDD"
     gene            complement(1656..2720)
                     /locus_tag="VFT03_07275"
     CDS             complement(1656..2720)
                     /locus_tag="VFT03_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEU4847984.1"
                     /translation="MKDQGPRKSIVGRGAAGNPNNRFERIEVEPDEIEGDEPRPETVY
                     LRDHSRSIIARNYSPDIGFDASINPYRGCSHGCIYCFARPTHEYLGLSAGLDFESRIL
                     VKQDAPELLRKELSSPRWNPQVLSMSGVTDPYQPVERRLRITRRCLEVLAEFRNPVVI
                     VTKNYLVTRDMDLLSELASHDAAAVAFSLTTLDDDLRRIMEPRTSRPTRRLAAIEKLA
                     AGGIPVGVMTAPVIPGLNDHELPNLLSAAAEAGATFAAYVPVRLPYAVAPLFEDWLAH
                     HFPERKDKVLNRIRSMRGGELNDANFGSRMRGEGLFAQHIAQLFSISCRRAGMGEGGF
                     PKLSTAAFRGGGGVQPDLFG"
     gene            complement(2786..3865)
                     /locus_tag="VFT03_07280"
     CDS             complement(2786..3865)
                     /locus_tag="VFT03_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEU4847985.1"
                     /translation="MAERDGSSKRPVSERTVYEPRLAVRRIERTLLALGAIMVIGVVG
                     YIVFEGWSFTDAVYMTVITVTTVGYREVRPLDTTGQLWTMVLLITGVGTLFYAAVSSV
                     ELVVEGAIRGYFGRRRMQAAVGKLNGHYILCGYGRVGRQVAREFARDGVPFVVVDQEP
                     GIIEECLVEGYLALLGEASDDTVLEEAGVRRARGLVAAVDSDADNVFVVLSARKLNPK
                     LNIVARASSDESAAKLEIAGADRTLSPYAVGGRRLASLATQPLVVDFLDIVTRGEKGL
                     EFRLEEFGVPEDSFIAGQTIGELRIGERTGAMILATRNSEGTFDTSPSASDRIRAGDT
                     LIVLGTREQISRLERLMRGEGVSED"
     gene            complement(3902..4378)
                     /locus_tag="VFT03_07285"
     CDS             complement(3902..4378)
                     /locus_tag="VFT03_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU4847986.1"
                     /translation="MSPLAVVIADTHIPRKARALPEGLTPYLERADLVLHAGDLMDLA
                     LLDQLAAYAPVEAVRGNMDLPGLPETLELELGGVRVAMIHDSGPGRGRRARARRRFPD
                     ARVVVFGHSHIPLLEDEDGLMLLNPGSPTDRRRQPRHTFALLRVQEGEVRAEILPL"
     gene            complement(4436..4594)
                     /locus_tag="VFT03_07290"
     CDS             complement(4436..4594)
                     /locus_tag="VFT03_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4847987.1"
                     /translation="MRASIEYRLGHRHDDRSVLRGSVNKKGMDIAAITQFATAVVQFV
                     FVVVIGVS"
     gene            4593..5405
                     /locus_tag="VFT03_07295"
     CDS             4593..5405
                     /locus_tag="VFT03_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU4847988.1"
                     /translation="MRPISFISDYGLTDVLVGTCKGVIISIAPGAPIIDVTHTVPEFD
                     VIRGAETLLHATRYMPEDSVYLAVVDPGDNTRRRALAAEVRSGAYLVGPDNGLLLPAA
                     EALGGIARVVHLTNPRYHVQPVSSTFHGRDVFSPVAAHLAAGADLDDLGERVDPASMV
                     SLDFPGFRREADGSLTAKIINIDRFGNARLSVMQEDLDLQFGTPLEIGVRDEVIQARY
                     VETFGTAEDGDLVVVPDSHWRLSLAVNKGNAARALLLSIGEEVHLKPPAERA"
     gene            complement(5412..6230)
                     /locus_tag="VFT03_07300"
     CDS             complement(5412..6230)
                     /locus_tag="VFT03_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4847989.1"
                     /translation="MERNVGNGRMTICQISDIHCGNARFIPELLDRTIIEINRSNPAT
                     VVVSGDLTDAGYREEFEQAAEYIDRIECESIMVIPGNHDSRNVGYVHFERLFGERQSV
                     IDLDEVILVGVDSSEPDLNDGQVGREHYDFIEKSFSDAVDKLKIFVVHHHLIPLPGTG
                     RERNIIFDAGDILERLIETEVDLVLSGHKHVPYTWRLENMLIVNAGTASTLRLRGNTR
                     PCYNVIEVEDGCVEVYRKYPFKERELAVRFDRNAREYSRFENKIDAEITGHPKK"
     gene            complement(6247..>6894)
                     /locus_tag="VFT03_07305"
     CDS             complement(6247..>6894)
                     /locus_tag="VFT03_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4847990.1"
                     /translation="ATSQAILETAVEVVRQSGWDGPVGCGFPAVIKGGVVQTAANIDK
                     SVIGFDMQGRLEQELGNPVRVINDGDAAGLAEMRWGAGREEDGVVLMLTLGTGIGTSV
                     FVGGRLLPNTELGHIEIRGEDAEHRASDSARKRDDLSWKKYAGRLDEYLHKIEDLLWP
                     DLIVIGGGISKKSEKFFPHLTARTPIVRAQMLNEAGIAGAALAAIPDKSPVQAAE"
BASE COUNT         1200 a         2228 c         2190 g         1276 t
ORIGIN      
        1 acgccctcga caaggccttg cgacaggcca tagagccgca ttacccggag ctgaaggaga
       61 tacacctctc caactacaag gtccgcatcc tcgacgagca cagggcgacc gccgcgacga
      121 cgcgggtcct catagactcg accgacggca agcgtgtctg gggcgccgtg ggagtaggcg
      181 agaacattat cgaagcttcc tggcaggcgc tcgtggacgg gctcgagtac ggtgtcaaca
      241 ggaccgggaa ggactgaagt agcatttcag cacgcccgct tcgcgggctt gtcagctaga
      301 aagcaaggag tacgcgacat gatgcttccc cgcgctaatc caaccttatg cccaaaagca
      361 tctctccaag cgtgatccgg gcaagagccc aacggcagag agaaagctga caagcgaggg
      421 ccttctaaag gcccgagcgt gctgacaagc cgaccgaagg gaggcgtgct agagcgtagc
      481 gcttcagtcg tcgaacggtc cgccgctgag ctggacgggg tccttgccgc cgacgttcac
      541 atttacgttg accacgccat cgttccgagg cggcgggcta ctgttgcccc cagagggcgc
      601 tcctccaggc tctctggtcg ggggagggct cgatggggaa gggctcgatg gggaagggct
      661 cgatggggaa gggctcgacg gggaagctgt gtcatcctga cgggtggggg cctcgatgct
      721 cgcggcattc ctccgggacg gttccgtggt cgcaggcttc ctgtcttccc gggctgaggc
      781 ggctgacgcc gtcgctgcgg gtgatgccgt ggcctgtgcg cttgcggagt tctggggcat
      841 tggatccgcc gccttatcct cggccgcaga ggcggagcgg tcggagtgcg acgtaccttt
      901 ctcgtcggcc gtactccgtt gcttatcggc ctcgttcgta cctttttgct catcgtcctg
      961 ctgcgggttc tggacgctgg cgtgctcctt tgcgcctgcg ggagaggcct cgggcgcccg
     1021 ggaggtcaag ggttcgcgta cctgggagac cgccgacggc cgctcatccc ccgcgacggt
     1081 gagcgcgagg gccaccacgg ccgcgcagag gagcaggaat acagcagagc cgaggtacaa
     1141 gcgcgcgtcg aagcccgcgg gacgcggggt cggcacggtc tcttcttcgt ccctggtcgc
     1201 atcccggctt ttcgcagcac cgcccagggc ggtcaggatg tcgtcgaggg agtcatcctc
     1261 actggggccg acgtacaacg ggtatggggg ctcctcggag agagactgcc ggcgtttgag
     1321 ttccccgacc atcgcgtcgg cgtcaaggtg aaggaagctg gcgtaggtct tcaggtagcc
     1381 ttgcatgtac acggcgggca gcacgtcgaa gtgctcccac tccaggtcag ccaggtacac
     1441 ggcacggatc ttggtagcct cttccacctg tttcaaggag agaccccgcc tcttgcgtgc
     1501 cagctccaga gtcctgccta tctcgagggg cgcgtcgcta cctccgccgc tcatgtccgc
     1561 caaaccagaa gtcttctctc tcgtaggtga tccatgcctc acccatgata cctccgaagc
     1621 gctcaacctc acaccccgca ggaattattt ttccgtcagc cgaagagatc gggctgcact
     1681 ccgccgcctc cgcggaacgc agcggtcgag agcttcggga agcccccctc tcccatgcct
     1741 gccctgcggc agcttatgga gaagagctgg gcgatgtgct gggcgaagag cccctccccc
     1801 ctcatgcgcg atccaaagtt agcgtcgttg agctcgccgc cgcgcatcga gcggatgcgg
     1861 ttcagcacct tgtctttccg ttcggggaag tggtgagcca gccagtcctc aaagagcggt
     1921 gcaacagcgt aagggagccg cacgggaacg taggcggcga aggtcgctcc ggcctccgct
     1981 gcggcggaga ggaggttggg aagctcgtgg tcgtttaggc cgggtatcac gggtgccgtc
     2041 atcacgccaa cggggatgcc gcccgcggcg agcttctcga tggcggccag ccggcgcgtg
     2101 gggcgggagg tcctgggctc catgatccgc cgcaggtcgt cgtccagggt ggtgagcgag
     2161 aaggcgaccg cggcggcatc gtggctggcg agctcggaga ggagatccat gtcgcgcgtg
     2221 acgaggtagt tcttggtaac gatgacgaca gggttgcgaa actccgcgag gacctcgagg
     2281 cagcggcgtg tgatccggag cctcctctcg acgggctggt aggggtcggt aacgccgctc
     2341 atcgagagca cctgtgggtt ccagcgggga gatgacaact cttttcgcag cagctcggga
     2401 gcgtcttgct tcaccaggat cctgctctcg aagtccaggc ccgcggagag acccaggtac
     2461 tcgtgggtgg ggcgagcaaa acaataaata caaccatggc tgcatccacg gtacgggttg
     2521 atgctcgcgt cgaagccgat gtcggggctg tagttgcggg cgatgatgga gcgcgagtga
     2581 tcccgcaggt agacggtctc gggccgcggc tcgtctcctt cgatctcgtc aggctcgacc
     2641 tctatcctct cgaagcgatt attcgggttg ccggcagccc cgcgaccaac tatgctcttc
     2701 cgtggcccct gatccttcac ccaccgattc taatggcgag gaccttcgat accaccgcgc
     2761 gttttgcgct gcgaaacaaa ggctactagt cttcgctcac tccttcgccc ctcataaggc
     2821 gttcgaggcg tgagatctgc tccctggtac cgaggacgat gagggtgtcg ccggcgcgaa
     2881 tgcggtcgct ggcggaaggg ctggtgtcga aggtgccctc actgttgcgg gtggccagga
     2941 tcatggcgcc ggtccgctcc ccgattcgga gctcgccgat ggtttgaccg gcgatgaacg
     3001 agtcctcggg cacgccgaac tcctccagcc tgaactcgag ccctttctcg ccccgggtga
     3061 cgatgtccag gaagtccacg accagaggct gcgtggcgag cgaggcgagt cgcctgccgc
     3121 caactgcgta gggggagagg gtgcggtcgg cgccggctat ctcgagcttg gctgcgctct
     3181 cgtccgagct ggcccgggcg acgatgttga gcttcgggtt gagctttctg gccgagagca
     3241 cgacgaagac gttgtccgcg tcggagtcta cggctgccac cagccccctg gcccgccgga
     3301 cgccggcctc ctcgagtacg gtgtcgtcgg aggcctcgcc gagcagagcg aggtatccct
     3361 ccaccaggca ctcctcgatt atccccggct cctggtcgac cacgacgaag ggcacgccgt
     3421 cgcgggcgaa ctcgcgcgcg acctggcgcc ctaccctacc gtagccacac aggatgtaat
     3481 gtccatttag cttgccgaca gccgcctgca tccgccgcct tccgaaatag cccctgatcg
     3541 cgccctccac aacaagctcc acggaggaga cggccgcgta gaaaagggtg cccaccccgg
     3601 ttatgaggag gaccatggtc cacaactgcc ccgtcgtatc gagggggcgt acctccctgt
     3661 agcctacggt agtcaccgtg ataaccgtca tgtacaccgc gtcggtgaag ctccagccct
     3721 cgaatactat gtagcccacc acgccgatca ccatgatggc gccgagcgcc agaagggtcc
     3781 tctcgatacg cctcacagcc agccgcggct cgtaaaccgt cctctctgag acaggccttt
     3841 tgctcgatcc gtcacgttcc gccatcgcca gctattatag tggggcgctc cggataccgg
     3901 cttataaagg cagtatctcc gcccgcacct ctccttcctg cacgcgcagc agcgcgaagg
     3961 tgtggcgcgg ctggcgccgc ctgtcggtcg ggctaccggg gttgagcagc atgaggccgt
     4021 cctcgtcctc caggagaggg atgtgcgagt ggccgaagac gacgacgcga gcgtccggga
     4081 agcggcgcct cgcgcgagcc cggcgaccgc gtccggggcc agagtcgtgg atcatggcga
     4141 cgcggacgcc gccgagctcg agctccaacg tctccgggag gcccggtaga tccatgttcc
     4201 ctcgtaccgc ctcgacgggc gcgtaggcgg cgagctggtc gaggagcgcc aggtccatca
     4261 ggtcccccgc gtgcagcacc aggtcggccc gctcgaggta cggggtcagg ccctccggca
     4321 gggccctggc cttgcggggg atgtgggtgt cggcgatgac gacggccagt gggctcacac
     4381 tgacagggtc tcacacttca gcacgctccg gctgacacct ccgcttgtca gcacttcagc
     4441 tgactccgat caccaccacg aagacgaact gcacgacggc ggtagcgaac tgggttatcg
     4501 cggcgatatc catgcccttc ttattaaccg agcctcgcaa gacgctccgg tcatcgtgcc
     4561 ggtgtccgag acggtactct atacttgctc gcatgaggcc cataagcttc atatcggact
     4621 acggcctcac agacgtgctg gtggggacct gcaagggcgt aataataagc atcgcgcccg
     4681 gagcccccat aatagacgtg acccacaccg tccccgagtt cgacgtcatc agaggggcgg
     4741 agacgttgtt gcacgccacc cggtacatgc ccgaggacag cgtctatctg gccgtggtag
     4801 acccggggga caacaccagg aggcgagcgc tcgccgccga ggtccgcagc ggcgcctacc
     4861 tggtcggccc ggacaacggg ctcctcctgc ccgcggccga agcccttggg ggcatagcac
     4921 gggtggtgca cctcaccaac ccgcgctatc acgtgcagcc cgtctccagc accttccacg
     4981 gccgggacgt attctccccc gtggcggctc acctggcggc gggagcggat ctcgacgatc
     5041 tgggagaaag ggtggatcca gcctcgatgg tatcactcga cttccccggg ttccggcggg
     5101 aagccgacgg cagtctcacg gccaagataa tcaacatcga tcgttttggc aacgcccgtc
     5161 tctccgtgat gcaggaggac cttgacctcc agttcggcac gcccctggag atcggagttc
     5221 gggacgaggt catccaggcg aggtacgtgg agaccttcgg gaccgcagag gacggtgacc
     5281 tggtagtggt ccccgactct cactggcgcc tgagcctcgc cgtgaacaag ggcaacgccg
     5341 cccgagccct tctgctgtcc atcggagaag aagttcacct caagcccccg gcggagagag
     5401 cctgatctcg ctcacttttt agggtggccg gtgatctcgg cgtctatctt gttctcgaag
     5461 cgcgagtact cccgggcatt ccggtcgaag cgcaccgcga gctcgcgctc cttgaagggg
     5521 tacttgcggt aaacctccac gcacccgtct tcgacctcga tcacgttgta gcacggacgg
     5581 gtgttgcccc tgaggcgcag ggtcgaggcc gtccctgcgt tcacgatcag catgttctct
     5641 aatctccagg tgtacggcac gtgcttgtgc cccgagagaa cgagatccac ctccgtctct
     5701 atgagcctct ccagaatgtc gccggcgtcg aagatgatgt ttcgctcgcg cccggtcccc
     5761 gggagcggga tgagatggtg gtgaacgacg aatatcttga gtttgtctac cgcatcggag
     5821 aatgacttct cgataaaatc gtagtgctcc cggcccacct gcccgtcgtt gaggtccggc
     5881 tccgaggagt ccactcccac caggatcacc tcgtcgagat ctatgaccga ctgacgctcc
     5941 ccgaagaggc gctcgaagtg gacgtagccg acgttgcgcg agtcgtggtt gcccgggatg
     6001 accatgatac tctcgcactc gatacgatcg atgtactctg cggcctgttc gaactcctcc
     6061 ctgtatcccg cgtcggtgag gtcccctgag acgacgaccg tcgccgggtt cgagcggttg
     6121 atctcgatga tcgtccggtc cagaagctcg gggatgaagc gcgcgttgcc gcaatggatg
     6181 tcgctgatct ggcaaatcgt catccggcca ttacccacgt tccgctccaa ttccggcgtc
     6241 ggcctcttac tcggcggcct ggacagggga cttatcgggg atggcggcca gagcagcacc
     6301 cgcaataccc gcttcgttta gcatctgcgc cctgacaatg ggggtgcgcg ccgtgagatg
     6361 cggaaagaac ttctcgctct tcttgctgat ccctcctcct atcactatga ggtcgggcca
     6421 gagcaggtcc tctattttgt gaaggtactc gtccaggcgc ccggcgtact ttttccaact
     6481 caagtcatcg cgcttgcgcg cgctgtcaga ggcgcggtgc tcagcatcct cgcccctgat
     6541 ctcgatgtgt ccaagctccg tgtttggaag gaggcgtccc ccgacgaaca cagaggtgcc
     6601 tatccccgtg ccaagcgtga gcatgagcac cacgccatcc tcctccctcc ccgctcccca
     6661 gcgcatctcg gccagtcccg ccgcgtcgcc gtcgttgatg acacgaacag ggttgccgag
     6721 ctcttgctcg agcctccctt gcatgtcaaa gcctatgacc gacttgtcga tgttggcagc
     6781 ggtctgtaca accccgccct tgatcacagc agggaaaccg caccccacgg gaccatccca
     6841 cccggattgc ctcactacct cgacggcggt ctccaggatg gcctgtgaag tagc
//
LOCUS       DASTUV010000365         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1361755, whole genome shotgun sequence.
ACCESSION   DASTUV010000365 DASTUV010000000
VERSION     DASTUV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361755"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..914)
                     /locus_tag="VFT03_07310"
     CDS             complement(153..914)
                     /locus_tag="VFT03_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU4847991.1"
                     /translation="MLKLTIHHLYADMMNLYGDRGNVISIMKRCEWRGVPVEIVDVGL
                     GESIEPTGCDLFLFGGGQDREQALLAGDLSGSKGSDLRAIVEDGGVVLGVCGGYQLMG
                     HFYETPDGQKLPGVGVFDLYTEPRKPDEERLIGNVLIRVRGPEDEGMREVVGFENHGG
                     RTYLGDGVEPLGEVVVGFGNNGKDGTEGARRLNACGTYLHGSLLPKNPWLTDQLILNA
                     LRRVDDDFELEPLDDAAERNAFESVAARIAGRRTG"
     gene            complement(1040..>2268)
                     /locus_tag="VFT03_07315"
     CDS             complement(1040..>2268)
                     /locus_tag="VFT03_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEU4847992.1"
                     /translation="TNGKTTTTRMISRILQTAGMRAVNNSTGANLITGVTAALISDSN
                     LSGKPASEMGLFEVDEASVPKVASEADLGILAVLNLFRDQLDRYGELAYTGRVVASAF
                     PALPEDGSVVLNADDPLVASLGRHARKPVYYGVDEPSLDTGRLQHIADSKDCPICGTA
                     LAYDAVYMGHVGVYDCKACSFGRPELAYRASSVRLDGARGADFLLSTPTQEAEVRVNL
                     PGLYNVYNALAAATVAAEAGVGIDEISRGLRDFGGAFGRVERVRAGDREAFLLLIKNP
                     VGFNEILRTFVASDDARYILIAINDNHADGRDVSWLWDVDFEMLAEARVEGKTKGASP
                     FMVAGIRAEDMAVRLKYADLPVGTVIPDLKEAIRAALAATPPGQTLYVLPTYTAMLEI
                     RKTLSELGYTHPFWEE"
BASE COUNT          360 a          751 c          749 g          408 t
ORIGIN      
        1 atcgccgaga cctactggca gctccactcc caggacccga cggcctggac gctggagctc
       61 gaccttcgcc ccgccgtgga gaggttctga ggctcgccac ccggagctgg ggctatgaag
      121 aaggccggcc cctcgacgcg caccttgaga cctcagccgg ttcgtcgccc ggctatccgc
      181 gccgcgacgg actcgaaggc gttcctctcc gcggcgtcgt cgaggggctc aagctcgaag
      241 tcgtcgtcca ccctgcgcag ggcattcagg atgagctggt cggtgagcca ggggttcttc
      301 ggtaacagcg agccgtgcag gtaggtgccg caggcgttca ggcgcctggc gccctcggtg
      361 ccgtccttgc cgttgttgcc gaagcctact accacctcgc ctaaaggctc tacgccgtcg
      421 ccgaggtagg tgcggccgcc gtggttctcg aagccgacga cctcgcgcat cccctcgtcc
      481 tcagggccgc gcactcggat cagcacgttg ccgatcagac gctcctcgtc gggcttgcgc
      541 ggctcggtgt agaggtcgaa gacgccgacc cccggcagct tctgaccatc gggtgtctcg
      601 tagaagtggc ccatgagctg gtagccgccg cagaccccca gaacgacgcc cccgtcctcg
      661 acgatagccc gcaagtcgga acccttggag ccggagaggt ccccggcgag gagcgcctgc
      721 tccctgtcct ggccgccgcc gaagaggaag aggtcgcagc ccgtgggctc tatggactca
      781 ccaaggccga catctacgat ctctaccggg acaccgcgcc actcgcagcg cttcattatg
      841 gagatgacgt tcccgcggtc gccgtagagg ttcatcatgt ccgcgtagag gtggtgtatc
      901 gtgagcttta gcacgctgcc tcccttcggt cgtcagcttg tcagcttctc tttgcactgg
      961 caagctgaca aggcgtctgg cgaagccagc gccgtgctga caagcggagc cctaaaaggg
     1021 cgtagcgtgc tcacaaaagc tattcctccc agaacggatg cgtgtagccc agctcgctca
     1081 aggtcttgcg gatctcgagc atcgctgtgt aggtcggcag gacgtagagc gtctggcctg
     1141 gcggggtggc cgcaagggct gctcttatgg cttctttgag gtcggggatg acggtgccga
     1201 ccgggaggtc ggcgtacttc aggcgcacgg ccatgtcctc ggcccggata ccggcgacca
     1261 tgaatgggga ggcgcccttg gtcttgccct cgactcgagc ttcggcgagc atctcgaagt
     1321 ccacgtccca gagccacgag acgtccctgc cgtcggcgtg gttgtcgttt atggctatga
     1381 ggatgtagcg ggcgtcgtcg ctagcgacga aggtgcgcag gatctcattg aagcccacgg
     1441 ggttcttgat gaggagcaag aacgcctccc tgtcccccgc tctcacccgc tcgacccgcc
     1501 cgaacgcccc gccgaaatcc cttaaacccc gggagatctc atcgatcccg acccccgctt
     1561 cggcggcgac cgtagccgca gccagggcgt tgtagacgtt gtatagcccc gggagattga
     1621 cccgcacctc cgcctcctgt gttggcgtcg agagcaggaa gtccgcccct cgcgcgccgt
     1681 ccagccgcac actgctggcc cggtaggcaa gctcggggcg cccgaaggag cacgctttgc
     1741 agtcgtagac gccgacgtgc cccatgtaga cggcgtcgta ggcgagcgcc gtcccgcaga
     1801 tcgggcagtc cttggagtcg gctatgtgct gcaggcggcc ggtgtcgagg gaaggttcgt
     1861 ccaccccgta gtagaccggc tttctggcgt ggcgtccgag gctcgcgacc agcgggtcgt
     1921 cggcgttcag gacgaccgag ccgtcctctg gcagggccgg gaaggccgag gcgacgaccc
     1981 tgcccgtgta ggcgagctcc ccgtagcggt cgagctggtc ccggaacagg ttcaggacgg
     2041 cgaggatgcc gaggtccgcc tccgacgcaa ccttcgggac gctcgcttcg tctacctcga
     2101 agaggcccat ctcggaggcg ggtttcccgg agaggttgga gtccgagatc agggcggccg
     2161 taacacccgt gatcaggttc gcccccgtcg agttgttcac ggcccgcatg ccggctgtct
     2221 gcaagatcct ggagatcatg cgcgtcgtgg tggtcttgcc gttggtgc
//
LOCUS       DASTUV010000366         6855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1336946, whole genome shotgun sequence.
ACCESSION   DASTUV010000366 DASTUV010000000
VERSION     DASTUV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336946"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1301)
                     /locus_tag="VFT03_07320"
     CDS             complement(66..1301)
                     /locus_tag="VFT03_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU4847993.1"
                     /translation="MLALRYSKSVPRYLLMRAAAKRIKNLETSRFSPLKLEDIPEPSL
                     PTPEWVRVKPLLSGICGSDLGTLSSENSPYFSPITSPPFVLGHEVVGVVTEDRGDFRA
                     GDRVVLEPALGCAVRGLDPPCPYCAAGRYALCVNVAKGDIAPGIQTGFCRDTGGGWSD
                     GTLVAHPTQLHRVPEDVSDEAAAAIEPLACAVHAALENEPGPDETALVVGAGPVGLFV
                     VAALKRLTGVGRLICVAKHDRQRKEALRLGADEVVHPKETYTKLPAMLGTEAYRPEIG
                     KPVVMGGADKVFECVGSPGTMEDAVRLARPGGHVALVGMPPARSCLDLTALWYKEVSL
                     TGSYAYGVEEYGEERIKSFQLALRLAPEIQLETMVGPRFRLRDYREAIGAARSAGRNG
                     HIRVVFDYRGVRSQQRHQT"
     gene            complement(1303..3561)
                     /locus_tag="VFT03_07325"
     CDS             complement(1303..3561)
                     /locus_tag="VFT03_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEU4847994.1"
                     /translation="MLAEAYRGRSILLTGGTGFLGTALVEKILRSLPELGCLYLLIRP
                     SRERDAAARFENDVLGSAAFRRLRRELGDAFDEHVSRKVRVLEGDVHAPSLGLGEEDL
                     SELSREVDVVIHSAASVVFDAPLDAAVESNVHGTLDLLELARGWEKRPLFLHVSTAYV
                     AGMREGFAPEEPPGDTSPNGTALDPYEEVYHLEGVVREVEEASRERSLLRRFETEASR
                     ELGMVGEEEEVAERVDQLRRAWMRERLVERGTERARALGWHDVYTFTKSLAERMVVRE
                     RGETPLVIVRPAIIESSHGEPYPGWIQGSRMADPIIMAFAKGIIREFPGDPESLIDIV
                     PVDHVVNATLAAATRRPSAPEVFQVASGDRNPLRYRQLYEHVREYFQENPLRDSGGRP
                     IQPSEWTFPGRRAVERRLKAELVGLDVAGKVLSRMPEGHIVADLRGRIARAEKRARMS
                     LYYSRIYGPYSSATATFSTARTARLFESLPPQDREEFPFDITALDWKAWLQGTHLPAL
                     TTRPSRKKRRRAVAEEPGEVAAIFDVDGTLVGSNVVSYYAWLKMREMPAAARPLWAAA
                     FLSRIPYYWALDKVSRAHFNRAFYKNYAGWKPSRARHLGQESFPGFTLSRIYPDALQC
                     LREHKRMGHRVVLLSGALDFLLDPFKDLADDVLSASLAEEDGVYTGELSGAPVAGEAR
                     ARMLASFARKRGVDLGRSYAYADSISDLPMLEAVGNPVAVNPDRRLAAAAKDRGWQLR
                     HWSKNGSSPDKA"
     gene            complement(3551..4156)
                     /gene="plsY"
                     /locus_tag="VFT03_07330"
     CDS             complement(3551..4156)
                     /gene="plsY"
                     /locus_tag="VFT03_07330"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU4847995.1"
                     /translation="MLGVLLVLCGYLLGSVPTGLLVGRAFGVDVLKEGSGNIGMANVL
                     RSAGKWPAALTMLGDMLKGFVPVVLARALTDDGWVIAAVALAAVIGACWSVFLGFKGG
                     KAVATGAGTTIGLAPPVGLVLFALWWTVALTSRYTSLAAIVLMIVSPFAFLLTGQPLP
                     YVIYTVVGGVVVLWRHRENAKALLAGRERKFGERARKKRAR"
     gene            complement(4320..5840)
                     /gene="trpE"
                     /locus_tag="VFT03_07335"
     CDS             complement(4320..5840)
                     /gene="trpE"
                     /locus_tag="VFT03_07335"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU4847996.1"
                     /translation="MIGTDRLNLTPSLGEARALARDYDVVPLYAEFIGDLETPISAVL
                     KFSGEENVFLLESAEAAERFGRYSFLGFDPKRTLSYRAGVYTIVDADGVREVPAPDPF
                     RGLAEILGKKSVAPLPNLPAFVGGAVGYLSYDAVRYLERLPDAPPDDLDVPEAYFAVT
                     DSLVVFDHLRHKVLVISLVDAARLRDVEGEGFAAAYRRAADDIRRVAERLSAPLVRRA
                     LPSGSEGFGVSSNFTRERYEEAVERAKEYIRAGDAFQIVPSQRFAAEVGDLDPLLLYR
                     GLRTVNPSPYMTYLKLGDLSLVGASPEPLVRAEGRRVMTRPIAGTRPRGATPEEDALL
                     AEELLADGKERAEHVMLVDLGRNDLGRVSEIGSVELVSFMEVERYSHVMHIVSTVEGD
                     LRSDLTALDALAAAFPAGTVSGAPKVRAMEIIDELEPTRRGPYAGATGYYGVDGRLDT
                     CITLRTALLKEGVAYFQAGGGVVADSVPSLEYEETRNKARAMSRALEVARARELWL"
     gene            complement(5824..6453)
                     /gene="hisIE"
                     /locus_tag="VFT03_07340"
     CDS             complement(5824..6453)
                     /gene="hisIE"
                     /locus_tag="VFT03_07340"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEU4847997.1"
                     /translation="MSAVEEIRFDDRGLVPVIAQDATTNAVLTLAYANREAVEKTLSS
                     GEAHYYSRSRGELWRKGETSGNTQRVVEVRLDCDADALLYRVEPRGPACHTGEDTCFF
                     TTVEGTGIEAGGESPDLGWMVDKLAATIAQRHREMPEGSYTAKLLGEGKERVAQKVGE
                     EAVEVVVAALAEERLAEEAADLVYHLLVLLEERGLGTGDVAKVLHDRHG"
     gene            complement(6450..>6855)
                     /locus_tag="VFT03_07345"
     CDS             complement(6450..>6855)
                     /locus_tag="VFT03_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU4847998.1"
                     /translation="ERFGSQCVVLSVDVRRRASAPGGDVYLNGGRVNTEIDALSWLVE
                     GERRGAGEFVLNSMDADGTEGGYDLELISAVAGKTNLPIVASGGAGGPEDMISAVKAG
                     AGAVLAASIFHFGEWSIAEVKQHLREAGIPVR"
BASE COUNT         1089 a         2408 c         2245 g         1113 t
ORIGIN      
        1 gtctttatcg aggatggtga tgaagcctgg acggctcatt ttttctccct ctggtcgaaa
       61 aggcttcagg tctgatgcct ctgctgagac ctgacgcctc tgtagtcgaa gaccactctg
      121 atgtggccgt tgcgtccggc cgagcgcgcc gcgcctatgg cttccctgta gtcgcgcaga
      181 cggaagcgcg ggccgaccat ggtctcgagt tgtatctccg gggcgaggcg gagcgccagc
      241 tggaagctct tgatcctctc ctccccgtac tcttctactc cgtaggcgta ggagccggtc
      301 aggctcacct ccttgtacca cagcgccgtg aggtcgaggc aactgcgcgc gggcggcatc
      361 cctacgaggg cgacatgtcc gccgggccgg gcgagcctca ccgcgtcctc catcgtcccg
      421 ggcgagccca cgcactcgaa gaccttgtcc gcgccaccca tgacgaccgg cttgccgatc
      481 tccggtctgt acgcctccgt gccgagcatg gcgggtagct ttgtataggt ctctttcgga
      541 tgcaccacct cgtccgcgcc gaggcggagg gcttcctttc tctgccggtc gtgcttggcg
      601 acgcagatca gccgccccac gcccgtgagc ctcttcagcg ccgcgacgac gaagagaccg
      661 acgggaccgg cccctaccac gagcgccgtc tcgtcgggtc ccggctcgtt ctctagcgcc
      721 gcgtggaccg cgcaggcgag gggctctatc gccgcggccg cctcgtccga cacgtcttcc
      781 gggaccctgt ggagctgggt agggtgggct acgagagtgc catccgacca gcccccgccc
      841 gtgtcgcggc agaagccggt ctggataccg ggcgcgatgt cgcccttcgc aacgttcacg
      901 cacagagcgt accggccagc ggcgcagtag gggcacggcg ggtctaggcc gcgcacagcg
      961 cagccgaggg ccggctcgag taccacccga tcgccggcac ggaaatctcc tctgtcttcg
     1021 gttacgacgc caacgacctc gtgaccgagg acgaaggggg gggatgtaat cggggagaag
     1081 tacggcgagt tctcggacga gagcgtgccc aggtccgagc cgcagatgcc cgagaggagc
     1141 ggcttcaccc tgacccactc gggagtcggg aggcttggct cagggatatc ctccaacttc
     1201 agcggggaga agcggctggt ctccaggttc tttatgcgct tcgcggcggc ccgcatgagc
     1261 aggtagcggg gcacggactt gctgtagcgg agggcgagca tcctaggcct tgtctggaga
     1321 ggagccgttc ttgctccagt gccggagctg ccagcccctg tccttcgccg ccgcggctag
     1381 cctcctgtcg ggattgacgg caaccgggtt gccgacggcc tcgagcatcg ggaggtcgga
     1441 gatcgagtcc gcgtaagcgt aggagcggcc gaggtcgacg cccctcttgc gggcgaagga
     1501 ggccagcatc cgggcacgcg cctcgcccgc gaccggcgcg cccgagagct cgccggtgta
     1561 cactccgtcc tcctcggcga gcgaggcgga gagcacgtcg tccgcgaggt ccttgaaggg
     1621 atccagcagg aagtccagag cacccgagag caggaccacg cggtggccca tccgcttgtg
     1681 ctcccgcagg cattgcaggg cgtcggggta gatgcggctc agggtgaagc cggggaagct
     1741 ctcctggccg aggtgcctgg cgcgggaggg cttccagccc gcgtaattct tgtagaacgc
     1801 gcggttgaag tgggcgcggc ttactttgtc gagcgcccag tagtagggga tcctggagag
     1861 gaacgccgct gcccagagcg ggcgcgccgc cgccggcatc tcgcgcatct tgagccaggc
     1921 gtagtacgag actacgttcg agccgacgag cgtgccgtcc acgtcgaaga tcgccgccac
     1981 ctcccccggc tcctccgcca ccgccctcct ccgcttcttg cggctcgggc gggtagtgag
     2041 cgccggcagg tgcgtcccct gcagccaggc tttccagtcg agagccgtga tgtcgaacgg
     2101 gaactcctcc ctatcctgcg ggggcaggga ctcgaagagc cgcgcggtgc gggccgtgga
     2161 gaaggtcgcg gtagccgagg aataggggcc gtagatgcga ctgtaataga ggctcatcct
     2221 tgcgcgcttc tcggcgcgcg cgatcctgcc ccgcaggtcc gcgacgatgt gcccctccgg
     2281 catgcgcgag agcaccttgc ccgctacgtc caggccgacg agctcggctt tgagccggcg
     2341 ctccacggcc ctgcggcccg ggaacgtcca ttcgctgggc tgtatcggac gcccccccga
     2401 gtcccgcaat gggttctcct ggaagtactc tctgacgtgc tcgtagagct gccggtagcg
     2461 cagagggttg cgatccccag aggcgacctg gaagacctcg ggcgccgagg gccgtcgcgt
     2521 ggcggcggct agcgtggcgt tcacgacgtg gtccacaggg acgatgtcta tgaggctctc
     2581 ggggtcgccg gggaactccc tgatgatgcc cttggcgaag gccatgatga tggggtcggc
     2641 catcctggaa ccctggatcc agcccgggta aggctccccg tggctgctct cgataatcgc
     2701 cggtcttacg atgacgagcg gcgtctcacc cctctctctc acgaccatgc gctcggctag
     2761 tgacttggtg aaggtgtaga cgtcgtgcca gcctagagcg cgggcccgct ccgtaccccg
     2821 ctcgacgagg cgctcgcgca tccaggcccg cctgagctgg tccaccctct cggccacctc
     2881 ctcctcctcg ccaaccatcc cgagctccct acttgcctcc gtctcgaagc gcctgagcag
     2941 cgagcgctcg cgcgacgcct cctcgacctc ccggaccaca ccctcgaggt ggtacacctc
     3001 ctcgtaagga tccagggccg tcccgttcgg cgacgtgtcc ccgggcggct cctccggcgc
     3061 gaagccctca cgcatgcccg cgacgtaagc ggtcgagacg tgcagaaaga gcggcctctt
     3121 ctcccagccg cgcgcgagct cgagcaggtc cagagtgccg tggacgttcg actcgacagc
     3181 ggcatctaga ggagcgtcga agacgaccga ggcggcggag tggatgacga cgtcaacctc
     3241 gcgcgagagc tcggagagat cctcttcgcc tagccccaac gagggcgcgt ggacgtcccc
     3301 ttcgagcacg cgcaccttcc gtgagacgtg ctcatcgaaa gcgtcgccga gctcccggcg
     3361 gagcctgcgg aaggcggccg agccgagcac gtcgttttcg aacctcgcgg ctgcgtctct
     3421 ctcgcgggag ggacggatca ggaggtaaag gcaacctagc tccggaaggc tgcgcaggat
     3481 cttctcgacg agcgccgtcc ccaggaagcc ggtccccccg gtcagcagga tcgacctccc
     3541 gcggtaggcc tcagcgagca cgtttcttcc tcgccctctc gccgaactta cgctccctgc
     3601 ccgcgagcag ggccttcgca ttctccctgt gccgccacag gaccactact ccgccgacga
     3661 ccgtgtagat gacgtagggc agaggctggc cggtaagaag gaacgcgaat gggctcacga
     3721 tcatcagcac gatggcggcg agcgaggtgt agcggctggt gagcgccacg gtccaccata
     3781 gcgcgaagag caccagcccc acgggcggcg cgagcccgat ggtcgtcccg gctccggttg
     3841 ccacggcctt tccccccttg aaccccagaa acaccgacca gcaggcgccg atgaccgcgg
     3901 cgagggccac ggcagctatg acccacccgt cgtccgttag cgcgcgcgcc agcactacgg
     3961 gcacgaaccc cttgagcata tccccgagca tggtcaacgc ggcgggccac ttgcccgcgc
     4021 tgcgcaggac gttggccatt ccgatgttgc cgctgccctc cttgagcacg tccacaccga
     4081 aggcccgacc caccagtagg ccagtgggca cggaacccag caggtacccg cacaggacga
     4141 ggagaacccc taacatagcg gaaagagtat agcccaacgg aggcacgcgg gcttgcgcgc
     4201 gtgcgccgcc cggaggagct ccgaggcacg ccgagcggcg ccggtagagg aggtttattc
     4261 cgggcagacg ggccgcgcgc cggtacgcca gaacaggtcg agatccagcc tcatcccggc
     4321 tagagccaca gctcgcgggc cctcgcgacc tcgagcgccc gcgacatcgc gcgcgccttg
     4381 ttgcgcgtct cctcgtactc aagggagggt acggagtcgg cgacgacgcc gccgccggcc
     4441 tggaagtagg cgacgccttc tttgaggagc gctgtgcgga gcgttatgca ggtgtcgagg
     4501 cggccgtcca cgccgtagta gcctgtggcg ccagcgtagg ggccgcgccg ggtgggctcg
     4561 agctcgtcta tgatctccat cgcccgcacc ttcggcgccc ccgatacggt gccggccggg
     4621 aaagcggcgg ctagagcgtc gagcgcggtg aggtctgatc tcagatcccc ctccacggtc
     4681 gagactatgt gcatgacgtg cgagtagcgc tctacctcca taaagctcac gagctccacg
     4741 gagccgatct cgctcacccg cccgaggtcg ttgcgcccga ggtcaacgag cattacgtgc
     4801 tcggcccgtt ctttcccgtc ggcgaggagc tcttcggcca gaagggcgtc ctcctccggc
     4861 gtcgcgcccc tggggcgggt gcccgctatg ggacgggtca tcacgcgccg gccctcggcg
     4921 cgcaccagcg gctcagggga agctccgacg agggatagat ctcctagctt caaatacgtc
     4981 atgtacgggg acgggttgac cgtgcgcagg ccccggtaca ggaggagcgg gtcgaggtcc
     5041 ccaacctccg cggcgaacct ctgtgacggc acgatctgga acgcgtcccc tgcccttatg
     5101 tactctttcg ccctctccac agcctcctcg tagcgctccc tggtgaagtt cgaggagact
     5161 ccgaagccct cgctgcccga cggcagcgcc ctgcgtacca gcggggccga gagccgctcc
     5221 gcgacccgcc ggatgtcgtc ggcagcccta cggtaggccg ccgcgaaccc ctccccctcc
     5281 acgtcgcgca accgggctgc gtcgacgagc gagatcacca gcaccttgtg cctgaggtgg
     5341 tcgaagacga cgagcgagtc cgtgaccgcg aagtacgcct ccggaacatc aaggtcgtca
     5401 ggcggcgcgt caggaagccg ctccaggtag cgcacggcgt cgtaggagag gtagccgacg
     5461 gccccgccca cgaaggctgg caggttcggc aacggtgcga cgctcttctt gcccagtatc
     5521 tccgccagcc cgcggaaggg gtcgggagcg ggcacctccc tcaccccgtc ggcgtccacg
     5581 atggtgtaga cccctgcgcg gtacgagagc gtgcgcttgg ggtcgaagcc gaggaaggag
     5641 tagcggccga acctctcggc cgcctccgcg ctctcgagca agaagacgtt ctcctcgccc
     5701 gagaacttga ggacggcgga gatgggcgtc tcgaggtccc cgatgaactc ggcgtatagc
     5761 ggcaccacgt cgtagtccct cgcgagagcg cgggcctccc cgagagaggg cgtcaggttg
     5821 agcctatccg tgcctatcat gtaacacctt cgctacgtcg ccggtcccca agccccgctc
     5881 ctccagcaat accaggagat ggtacacgag gtccgcagcc tcctccgcga gccgctcttc
     5941 cgcgagcgcc gccacgacga cctcgaccgc ctcctctccg accttctggg ccacgcgctc
     6001 cttcccctcc ccgagaagct tcgccgtgta agagccctcc ggcatctcgc ggtggcgctg
     6061 cgcgatcgtc gcggcgagct tgtctaccat ccaaccgaga tccgggctct cgccgccggc
     6121 ctcgataccc gtcccttcca cggtcgtgaa gaagcaggta tcctcgccgg tgtggcaggc
     6181 cgggccgcgc ggctcgaccc tgtagagaag ggcgtcggcg tcgcagtcta gcctcacctc
     6241 gaccaccctc tgggtgttgc cgctcgtctc tcccttgcgc cacagctcgc cgcgggagcg
     6301 cgagtagtag tgagcctctc cgctggagag ggtcttctcg acagcctcgc ggttcgcgta
     6361 ggccagcgtc aggacggcat tcgtggtcgc gtcctgcgct atgacgggta cgaggcccct
     6421 gtcatcgaac cggatctcct ccaccgcgct caccggaccg gtatccccgc ctctcgcagg
     6481 tgctgcttga cctccgctat gctccactcc ccgaagtgga agatgctcgc ggcgagaacc
     6541 gccccagccc cggctttgac cgcggagatc atgtcctccg gcccccccgc cccgccggag
     6601 gcgacgatgg gcaggttggt cttcccggcg acggcggaga tgagctcgag gtcgtagccg
     6661 ccctccgtac cgtcggcgtc catgctgttg aggacgaact cgccagcccc ccgtctctcc
     6721 ccctcgacga gccacgagag agcatctatc tccgtgttca cgcgtccgcc gttcaggtag
     6781 acatccccac caggggcgga cgctcggcgc ctgacgtcca cgcttaggac gacgcactgg
     6841 ctcccgaagc gctcc
//
LOCUS       DASTUV010000367         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_150687, whole genome shotgun sequence.
ACCESSION   DASTUV010000367 DASTUV010000000
VERSION     DASTUV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150687"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFT03_07350"
     CDS             complement(<1..405)
                     /locus_tag="VFT03_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU4847999.1"
                     /translation="MRRPLIHGRYALGDLLGGGGMAQVYLARDEVLGRDVALKILRER
                     YAHDEPFVERFKREARSAAALNHPNIVQVYDQGQTEDGDHYIAMEYVPGGTLAQRIKR
                     EGPLDPREAAGLASRVADALAVAHGHGIIHRDI"
     gene            complement(519..1097)
                     /locus_tag="VFT03_07355"
     CDS             complement(519..1097)
                     /locus_tag="VFT03_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEU4848000.1"
                     /translation="MSVEPVRLETPLEARDLEPLRSGDVVRLYGVLYTARDAAHDRMK
                     WAIEREETLPFDPEGQVVYFTGPAPARPGSALGPAGPTTASRMDPYSPYLIERGLRGM
                     VGKGARSEEVLESMREHGCVYFGAVEGTAALLADRVKEAGVVAYEDLGPEAVRRLLVE
                     DFPVVVINDLHGGDLYREGRERWRRSGGGGTG"
     gene            complement(1094..1936)
                     /locus_tag="VFT03_07360"
     CDS             complement(1094..1936)
                     /locus_tag="VFT03_07360"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU4848001.1"
                     /translation="MREIEAAEITEAVRELCIEANTRLPEDHLAALHRALEEEESPLG
                     REVIGELLENARVARERCIAFCQDTGYAVFFVELGSEARVVGGGIGEAIDEGVRRGYD
                     EGYLRKSIVRGPVDRTNTGDNTPAIVYYDVVPGDGLRLTLLVKGAGCDNMSSLKMLTP
                     AEGVEEMKRFVVETVERAGPNASPPLTVGVGIGGPFERAALLAKKALTRRSGEPNPDP
                     ELAGLEAELLEAINATGIGPAGYGGTVTALAVHIESFPTHIAAFPVAVNLDCHSHRTS
                     SVEL"
     gene            2326..3495
                     /locus_tag="VFT03_07365"
     CDS             2326..3495
                     /locus_tag="VFT03_07365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEU4848002.1"
                     /translation="MTAQTKNKRGFWQNLLRRDGRRTPDETPAVAEVPQQAAPELEIA
                     PNDPILTYLASVSGVVEVEKLDLDSPALAAMKEAGVKLVVPLVSQGELIGILNLGPRL
                     SQQEYSADDRKLLSDLATQTAPAVRVAQLVRQQQQEAQERERIEQELKVARLIQQTLL
                     PKTLPELPGYDVAAYYQPAREVGGDFYDFLDLEDGRMGFVVGDVTDKGVPAAIVMATT
                     RTMLRAAAQRLFSPGEVLKRVNDVLVTDIPPNMFVTCLYAILDPESGRIIYANAGHDL
                     PYRRRAGRNEGAEELRATGMPLGLLPGMGYEEKEIVLEKGESVLFYSDGLVEAHDPRG
                     DMFGFPRLQGLVGAHRSGGSSLVGFLLSELARFTGEGWEQEDDITLVTLERSEEL"
     gene            3492..3953
                     /locus_tag="VFT03_07370"
     CDS             3492..3953
                     /locus_tag="VFT03_07370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4848003.1"
                     /translation="MTAPRSGARRTLAGLRVPSEPGNERRAMEEVAAAVEGLGLPDRT
                     LERLKTAVAEATMNAMEHGNHYRAELPVIIEVAASDAELSVKITDEGSGPPAFHSETP
                     DLEAKLEGMQTPRGWGLFLIKSMVDEMNVTGDEHHHTVELILHLDEREGGR"
     gene            3953..4303
                     /locus_tag="VFT03_07375"
     CDS             3953..4303
                     /locus_tag="VFT03_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4848004.1"
                     /translation="MAVRHLEVEVRREPGGAVLDLRGEINGFAQEALDAAYTEAEKEN
                     PEAIILNFEGVDYINSTGIALIVGLLARARATRRRLVAYGLSDHYVEIFNITRLSDFV
                     SVFPDEESALEPAT"
     gene            4390..4743
                     /locus_tag="VFT03_07380"
     CDS             4390..4743
                     /locus_tag="VFT03_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4848005.1"
                     /translation="MPQAQVTMEVRKVSANVSIIDVKGELTAFAEGVLMDAYGQASDG
                     RVNAIVLNFERLEYMNSSGIGLLVTLLIRVNREKQRLLTYGLSEHYRSIFQITRLDDA
                     IGIYDSEEEAVRAAT"
     gene            4764..>5355
                     /locus_tag="VFT03_07385"
     CDS             4764..>5355
                     /locus_tag="VFT03_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848006.1"
                     /translation="MTERHRDAAHWARYVETLKAPDGIVHRNVEGRRLVSPLQGFGKM
                     WQKTYRVRLEDASPSEVVATWRERFPEISGFEEGFRVPSGGLAPGAVALLGGLSGVMV
                     LYSDETSFTYMTPEGHPFSGWITFSAHRDEDSATVAQAQLLIRANDPLYELMMPLGLH
                     RLEDRTWQRTLHNLAARFGGDGQVETRVVCVDPKRQW"
BASE COUNT          947 a         1746 c         1798 g          864 t
ORIGIN      
        1 tatgtcgcgg tggattatgc cgtgcccgtg cgcgaccgcg agggcgtcgg cgacccgtga
       61 cgccaaccct gcggcctcgc gcgggtcgag ggggccttcg cgtttgatcc tctgggcgag
      121 cgtaccgccg ggcacgtact ccatggcgat gtagtggtcg ccgtcctcgg tctggccctg
      181 gtcgtagacc tgcacgatgt tggggtggtt gagcgccgcg gcgctcctgg cctcgcgctt
      241 gaagcgctcg acgaagggtt cgtcgtgggc gtagcgctcc ctgaggatct tgagcgccac
      301 gtcccggccc agaacctcgt cacgggccag gtacacctgg gccatgcctc cgccgcccag
      361 gagatctccg agggcgtagc ggccgtggat taggggtcgc cgcatgcagc gcatggtatc
      421 atcgccgccc cgttccttgc ggtcggcgat cgcgtttaca tgatggacgt tcgccgggac
      481 ttagactcgc ggagagcgca tgctcggtgg tatgcgggct acccggtgcc accgccaccc
      541 gaccggcgcc acctttcgcg gccctcacgg taaaggtcgc ctccgtgcag atcgtttatc
      601 acgacgacgg ggaaatcctc cacaaggagc ctgcggacgg cttcggggcc gaggtcttcg
      661 taggcgacta ccccggcctc cttcacgcgg tcggccagga gcgctgccgt cccctccacg
      721 gcgccgaagt agacgcaacc gtgctcgcgc atgctctcca gaacctcctc ggagcgcgcg
      781 cccttgccca ccatccccct gaggccgcgc tcgatcaggt aaggcgagta ggggtccatc
      841 ctcgaagctg tcgtcggtcc cgctggcccg agggcgctgc cgggacgcgc cggcgccggg
      901 ccggtgaagt acactacctg cccctccggg tcgaacggca gcgtctcctc cctctcgata
      961 gcccacttca tgcggtcgtg ggccgcgtcg cgcgcggtgt aaaggacccc atagagtcgc
     1021 acgacgtcgc cgctcctcaa cggctccagg tcgcgcgctt cgaggggcgt ctccaggcgg
     1081 actggctcga cgctcacagc tctaccgagg aggtgcggtg ggagtggcaa tccaggttca
     1141 cggcgacggg gaaggcggcg atgtgggtcg ggaagctctc gatgtgtact gcgagcgctg
     1201 ttaccgtacc gccgtagccg gccggcccga tgcctgtggc gttgatggcc tccagtagct
     1261 ctgcttccag gcccgccagc tcggggtcgg ggttgggctc ccccgagcgg cgggtgaggg
     1321 ccttcttggc gagcagggcc gccctctcga aggggccccc gatcccgacg cccacggtaa
     1381 ggggcgggct cgcgttcggg ccagccctct caacagtctc gacgacgaag cgcttcatct
     1441 cctcgacgcc ctcggccggg gtgagcatct tcaaggagct catgttgtcg cagcctgctc
     1501 ccttgacgag aagcgtcaac ctgagcccgt cgccgggcac gacatcgtag tagacgatcg
     1561 ccggagtgtt gtcgccggtg ttggtacggt ccaccggacc tctgacgatg gatttgcgca
     1621 agtaaccctc gtcgtagccg cggcgaacac cttcgtctat cgcttcgccg atcccgcctc
     1681 ccactacacg cgcctcgctt ccgagctcga cgaagaagac agcgtagccc gtgtcctggc
     1741 agaaggcgat gcaccgctcg cgggccaccc gcgcattctc gagaagctcc ccgatcacct
     1801 cccgccccag aggagactcc tcctcttcga gcgccctgtg cagcgccgcc aggtgatctt
     1861 caggcaggcg cgtgttcgcc tcgatgcaca actcgcgcac cgcctcggtt atctcagccg
     1921 cctctatctc tcgcaatcct cacctcgatg tcgcgtcgtc gcgtgtagcg tagcatcaga
     1981 gcgcctctct gggggcgcac cgcggctcag gagatgctac tcgcggtcag ggtagagcca
     2041 gaaagggaag aggcgcgtca ggcggggcaa tgaccagcgt gcgctccagg gcggggagta
     2101 gattcagcca ctggccgatc acggataaga tgatcgccat cttgtagcga ggtggtgcgg
     2161 gcgacgatgc ggccggcacg tcgctctggc tgagagatcc ggaggagatg aggggggaga
     2221 aaggtgaggc gaagtgcgag aaaatcccta ggagtggagt tccgtaacgc ttttcgtaac
     2281 gctccggaga cggtcgacac ctagaatggt gggcaaggag gtcagatgac ggcacagacc
     2341 aagaacaagc gggggttctg gcagaacctt ttgaggcgcg acgggcgcag gacacccgac
     2401 gagacgcccg ccgttgcgga ggtgccacag caggcggcgc ccgagctcga gatagccccg
     2461 aacgacccta tcctcacgta cctcgcgagc gtctcggggg tggtcgaggt cgagaagctg
     2521 gacctcgact cgccggcgct cgcggcgatg aaggaggccg gcgtcaagct ggtggtcccg
     2581 ctcgtgagcc agggtgagct catagggatc ctcaacctcg ggccgcgcct ctcccagcag
     2641 gagtactcgg ccgacgaccg caagctgctc tcggatctgg cgacccagac ggccccggcc
     2701 gtcagggtgg cccagctcgt caggcagcag cagcaggagg cccaggagcg ggagcgcatc
     2761 gagcaggagc ttaaagtcgc gcgcctgatc cagcagaccc tcctgccaaa gaccctgccg
     2821 gagctgcccg gctacgacgt tgcggcctac taccagcctg cccgcgaggt aggcggcgac
     2881 ttctacgact tcctcgacct cgaagacggc cgcatgggct tcgttgtcgg ggacgtcacg
     2941 gacaagggtg ttcccgcagc catagtcatg gcgacgaccc gcaccatgct ccgcgccgcc
     3001 gcccagcgcc tcttctcgcc gggggaggtc ctgaagcggg tcaacgacgt cctcgtcacc
     3061 gacataccac ccaacatgtt cgtcacctgc ctgtacgcga tcctcgaccc cgagagcggg
     3121 cgtatcatct acgccaacgc gggccacgat ctgccctacc ggcgtcgcgc cgggcgtaat
     3181 gagggcgctg aagagctcag ggcgacgggg atgccgctcg ggctcctgcc ggggatgggt
     3241 tacgaggaga aggagatagt cctggagaag ggcgagagcg tcctgttcta cagtgacggc
     3301 ctcgtcgagg cgcacgaccc tagaggcgac atgttcggct tcccacggtt gcagggtctt
     3361 gtaggcgccc accgctcggg cggctcctcg ctcgtcggct tcctcctctc cgagctcgcg
     3421 cgcttcaccg gcgagggctg ggagcaggag gacgacatta cactggtgac cctggagaga
     3481 tcggaggagc tgtgacggca cccaggagcg gcgccagacg gacgctcgcc gggctgagag
     3541 tgcccagcga gcccggcaac gagcgccggg cgatggagga ggtggcagcg gccgtggagg
     3601 gactcgggtt gccagacagg accctcgagc ggctcaagac cgccgtggcc gaggccacga
     3661 tgaacgcgat ggagcatggc aaccactacc gggcggagtt gcccgtcata atagaggtgg
     3721 cggcctccga cgccgagcta tcagtcaaga tcaccgacga ggggagtggc ccgcctgcct
     3781 tccactccga gacgccagat ctcgaagcca agctggaggg catgcagacc ccgcgcggct
     3841 gggggctctt cctcataaag agcatggtcg acgagatgaa cgtcacaggc gacgaacacc
     3901 accacaccgt cgagctgatc ctgcacctcg acgagaggga aggaggtagg taatggccgt
     3961 gcgacacctg gaggtggagg tgcgccggga acccggcggc gccgtgctcg acctccgagg
     4021 cgagatcaac ggcttcgctc aggaagccct cgacgccgcc tacacagagg cggagaaaga
     4081 gaacccggag gccataattc tcaacttcga gggggtcgac tacatcaact cgacagggat
     4141 agccctgatc gtcggcctgc ttgcaagggc gcgcgccacg aggcgccgtc tcgtggccta
     4201 tggcctgagc gaccactacg tcgagatctt caacatcacc cgcctctctg acttcgtgag
     4261 cgtcttcccc gacgaggaga gcgcactgga gcccgcgacg tgagcttcgc tgcgcagctg
     4321 cacggctcgc ttgagtcctt cgcagcgagt tcaagagact aagggagcta agccgggaaa
     4381 ggagaggaca tgccccaggc acaggtaacg atggaagtgc ggaaggtgag cgccaacgtt
     4441 agcatcatag acgtcaaggg cgagctgacc gcgttcgcgg agggcgtcct gatggacgcc
     4501 tacggccagg caagcgacgg gagggtcaac gcgatcgtcc tgaacttcga gaggctcgag
     4561 tacatgaaca gtagcggcat cggcctcctg gtcacgctgc tcatccgtgt caaccgtgag
     4621 aagcagcggc tgctgaccta tggcctctcc gagcactacc gcagcatctt ccagatcaca
     4681 cgcctggacg acgccatagg catctacgac tcagaggaag aggccgtccg ggcggcaacg
     4741 taacgacgtt gaaaggagcc gtcgtgacgg agagacatcg cgacgctgcc cactgggctc
     4801 gctacgtcga gacgctcaaa gcgccagatg ggatcgtgca ccgcaacgtc gagggccgca
     4861 ggctggtgag ccccctccag ggcttcggca agatgtggca gaaaacctac agggtgcgtc
     4921 tcgaggacgc ttcgccgtcc gaggtcgtcg cgacctggcg ggagaggttc cccgagatct
     4981 ccgggttcga ggaaggtttt cgcgtgcctt cgggcggtct cgctcccggc gccgtcgcgc
     5041 tgttgggggg gttgagcggg gtgatggtgc tctactccga cgagacttcc ttcacctaca
     5101 tgacgccgga gggacatcct ttttcgggtt ggatcacctt cagcgcccac cgcgacgagg
     5161 acagcgccac cgtggcccag gcccaactgc tcatacgcgc caacgacccg ctctacgagc
     5221 tcatgatgcc tctcggcctt cacaggctgg aggacagaac ctggcagcgt accctccata
     5281 acctggcggc ccgcttcggc ggggacggac aggtcgagac ccgcgtcgtc tgcgtggacc
     5341 cgaagcggca gtggc
//
LOCUS       DASTUV010000368         4378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_764849, whole genome shotgun sequence.
ACCESSION   DASTUV010000368 DASTUV010000000
VERSION     DASTUV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764849"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..902
                     /gene="fetB"
                     /locus_tag="VFT03_07390"
     CDS             129..902
                     /gene="fetB"
                     /locus_tag="VFT03_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEU4848007.1"
                     /translation="MEGLQTVGSGLLTLGLVAVAVALSLYQRLDLEKDIGVAVVRSFV
                     QLAAIGYVIDYVFGLESLGAVALLLAGMVLFAAWTSARRASGVPRSFMVAAGAIGVAA
                     VATLGVLLLLHIVPPTARYLIPLGGMVIGNSMNTASLTLTRLRDDIGEQRPKVEAALA
                     LGATSRQAVAPILRTALQRALIPLIDATKTTGIIFLPGAMVGMIIAGADPLEAVRLQI
                     VVLYMLLGSVSIAAILVGLLSYRSFFTPRHQLKTAPQEY"
     gene            complement(1073..1546)
                     /locus_tag="VFT03_07395"
     CDS             complement(1073..1546)
                     /locus_tag="VFT03_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4848008.1"
                     /translation="MEDQASTFPTRILLATDGSPHAQLAAMKAVDLAKSTSARLHVVA
                     VGRTFPEAVYDVYAETSSEDLRREAQEILDEQVRKIEEAGGSVAIAHLRMNERRDEAI
                     VHLAEEIGADLIVMGSRGFGGLRRALLGNVADSVVRHAHCPVLVVRPTHGVSSPF"
     gene            complement(1551..2135)
                     /gene="cysC"
                     /locus_tag="VFT03_07400"
     CDS             complement(1551..2135)
                     /gene="cysC"
                     /locus_tag="VFT03_07400"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEU4848009.1"
                     /translation="MENGTENQRGFTLWFTGLSGAGKTTVSEIVEHELKERGLRVEVL
                     DGDIVRTNLSKGLSFSRDDRNINVLRIGFVANLLTRNGVAVIVSAISPYKEARDQVRR
                     RIIDFVEVFVDVPLEVAAERDVKGLYKKAFAGEIDQFTGVSDPYEPPVAPDLVLKTDE
                     ETPEESARRVIEKLEFFGYLWSPSQDTEYNWSNR"
     gene            complement(2292..3188)
                     /locus_tag="VFT03_07405"
     CDS             complement(2292..3188)
                     /locus_tag="VFT03_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4848010.1"
                     /translation="MRNLVVLAIVGFVAQLVDGSLGMAYGVTSTTLLLAIAGLTPALA
                     STVVHISEVGTTLASGVSHWRFGNVDWSKIAWLAIPGGIGAFVGATVLVRFIDAAAAE
                     PIVAIFLFLLGAYVLARFAFNRTTNVVQNLPIPRAFLAPLGLIAGFLDAVGGGGWGPI
                     STPTLLSSGRMEPRKVVGTVDTSEFIVALAASIGFLISLSFAQVPWEIVAVLLGGGLV
                     AAPIAAWIVRHLNARVLGTAVGGFILLVNANTFLEAVGLDPALNVFVYGAILAVWITA
                     LYFAYAAIRRERKPVIETQAAE"
     gene            complement(3332..>4378)
                     /locus_tag="VFT03_07410"
     CDS             complement(3332..>4378)
                     /locus_tag="VFT03_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU4848011.1"
                     /translation="VHTLHELGYWSALIGEQHISKRPDVIGYDEVYKIPTNHTDDVVP
                     VTLDLLERASDKPFFLSVGFFETHREWFRPSSPKKANYVMPPPNLPDTPETRLDMAAF
                     QESARSLDRGIGAVLDALDAEGLTENTLVICTTDHGLAFPGAKATLYDRGLGVMLILR
                     GPGGFSGGKAIDALVSHIDLFPTVCDLVGAERPPWLQGESLLPLVNSEADEVRDAIFA
                     EKTYHVAYEPQRCVRTHRWKYIRRFDDEHPTPVLANIDDGPSKDVILSNGYAERPIPA
                     EQLYDLLYDPNEANNLAGDPTLAGVLQEMRDRLESWMRDTRDPLLHGPVPAPKGAELN
                     GRDQLSPTDPTTTV"
BASE COUNT          766 a         1344 c         1444 g          824 t
ORIGIN      
        1 gtcgagcgcg tcgccgaccg cgtcatggtc ctggtgagag gacgtagcga gggtgagtgg
       61 ggcagggatg agttcttctc cgggccgggg gagcgggcga ggcggtacat ctcggggagg
      121 tcgtgagaat ggaaggtctg cagacggtgg gatccggcct gctcacgctc gggctcgtcg
      181 ccgtggcggt ggccttgagc ctctatcagc ggctggacct ggagaaggac atcggggtcg
      241 ccgtggtccg ctcgttcgtg cagctcgccg ccatcggcta cgtcatcgac tacgtcttcg
      301 ggctggagag cctgggcgcg gtagcgttgc tcctggccgg gatggtcctg ttcgccgcct
      361 ggacctcagc ccggcgggcg agcggcgtgc cccgatcttt catggtggcg gcaggtgcca
      421 taggggtggc ggccgtggca actttggggg ttttgctcct cctacacatc gtgcctccta
      481 cggcccgcta cctcatcccg ctcgggggca tggtgatagg gaactccatg aacaccgcga
      541 gccttaccct caccaggctg cgcgacgaca tcggcgagca gcggccgaag gtcgaggcgg
      601 ccctcgctct gggagccacc agccgccagg ccgtcgctcc catcctgagg accgcgctgc
      661 agcgggccct gatcccgctc atagacgcca ccaagaccac gggcataatc ttcctgcccg
      721 gcgcgatggt gggcatgatc atcgccggcg cggaccccct ggaggccgtg cgactgcaga
      781 tagtcgtcct ctacatgctc ctcggaagcg tctccatcgc ggcgatcctg gtgggtctgc
      841 tctcctacag atccttcttc acgccgcgcc accagctcaa gacggcgccg caagaatatt
      901 gaagaatagt gaccacacca attctccagc taatcgattc tggcaagaag ccgggagagc
      961 ctgcacctct taaaacataa aagctgaccc tagctgacaa gccgaccgca gggaggcgtg
     1021 ctgagaagcg aagccctaaa agggcgtagc gtgctgaaat gcgcttcgcg cattagaaag
     1081 gactgctgac gccatgggtc ggccgtacga cgaggacggg gcagtgggcg tgacggacga
     1141 cggaatcggc gacgttgccc agcagggccc gtctcagccc gccgaaaccg cggctaccca
     1201 tcacgatcag gtcggcgccg atctcctcgg ccaggtgaac gatcgcctcg tcccggcgct
     1261 cgttcatcct gaggtgggcg atagcaacgc tgccacccgc ttcctcgatc ttccttacct
     1321 gttcgtcgag aatctcctgt gcttcgcgcc tgaggtcttc actactagtc tcggcgtaga
     1381 cgtcgtatac agcctcagga aacgtgcgcc cgaccgccac gacgtggagc ctcgcgctgg
     1441 tgctcttcgc gaggtctacg gccttcatgg ctgccagctg cgcatgaggg gagccgtccg
     1501 tggccagcag gatgcgcgtc ggaaaggtgc tagcctgatc ctccaaggtt ctagcggttg
     1561 ctccagttgt actcggtgtc ctgagaaggg gaccagaggt acccgaagaa ctcgagcttc
     1621 tctatgaccc tcctcgcgct ctcctcaggg gtctcctcgt ctgtcttgag gacgaggtcg
     1681 ggagcgacgg gcggctcata ggggtccgag acgccggtga actggtctat ctcccctgcg
     1741 aaagccttct tgtagaggcc cttcacgtcg cgctcggccg caacttcgag gggtacgtcc
     1801 acgaagacct cgacgaagtc tatgatcctg cgcctcacct ggtcgcgggc ctccttgtac
     1861 ggcgagatcg cggacactat cactgcgacg ccgttgcggg tgagcaggtt cgccacgaat
     1921 cctatgcgca gcacgtttat gttccggtcg tccctggaga agctcaggcc cttggacaga
     1981 ttggtgcgca cgatatctcc gtcgaggacc tctaccctta ggcctctctc tttgagttcg
     2041 tgctcgacga tctcgctcac agtggtcttg ccagctcccg agaggcccgt gaaccacagg
     2101 gtgaacccgc gctggttttc cgtgccgttc tccatgccag ctcctttctg ccttgtatcc
     2161 gccgctgctt gccgcgccat agtctacatg ccgggttacg ccatgtcaga cccggcgcag
     2221 cgcttcgagc gacgaatccg gccggggctg taccccggcc ggacgcgtta gcaggattcg
     2281 ttcttcgtgc tctactctgc agcctgggtt tcgatcaccg gcttgcgctc gcgccggatg
     2341 gcggcgtagg cgaagtagag cgccgtgatc cagactgcca ggatggcccc gtagacgaac
     2401 acgttcagag ccgggtcgag cccgacagct tctaggaagg tgttggcgtt gacgagcagg
     2461 atgaacccgc cgacagccgt cccgagcacg cgcgcgttga ggtggcgcac gatccaggcc
     2521 gctatcggag cggcaaccag gccaccgccg agcagaaccg cgacaatctc ccagggcacc
     2581 tgggcgaagc tcaacgagat gaggaaaccg atgctcgcgg ccagcgcgac tatgaactcg
     2641 ctggtgtcaa cggtgccgac aaccttgcgc ggctccatgc gcccggaaga gagcagggtg
     2701 ggggtggaga tcggccccca cccgccgccg ccaaccgcgt cgaggaagcc cgcgatcagg
     2761 ccgagcggcg cgaggaaagc ccgcggaatc ggcaaattct gcaccacgtt ggtggtgcgg
     2821 ttgaacgcaa agcgcgccag tacgtaggcc ccaaggagga aaaggaagat cgctacgata
     2881 ggctcggccg ccgcggcgtc aatgaaccgc acaagtaccg tggcccccac gaacgcgcca
     2941 atgccgccag gaatcgccag ccacgcgatc ttggaccagt caacgttccc aaaccgccag
     3001 tgcgaaaccc cactggccag cgtcgtgcct acctcggaga tgtgtaccac cgtggacgct
     3061 agcgccggcg tgagccccgc tatcgccagt aataacgtcg tagacgtcac cccataggcc
     3121 atccccagcg acccgtccac aagctgagcc acgaacccta caatcgctaa taccaccaag
     3181 tttcgcacct gttagcgcct ccctttgttc cgattgtcta cctataatat gggcaaaaca
     3241 caatacggcg agactgtaca cagccaggta gggactgtca agggcgatag gcgcaatttt
     3301 gcgaattttt gttaagttgg tgttgtcgtc tttacacggt tgtggtggga tcggtggggg
     3361 agagctggtc gcggccgttc agttcggcgc ctttcggggc gggaacgggt ccgtggagga
     3421 gtggatctct ggtgtctcgc atccaggatt cgagccggtc tcgcatctcc tggagcacgc
     3481 cggcgagggt gggatctccc gcgaggttgt tcgcttcgtt ggggtcgtaa agcagatcgt
     3541 agagctgttc tgcgggtatg ggacgctctg cataaccgtt ggagaggatg acgtccttgc
     3601 tcgggccgtc gtctatgttg gctagtacgg gtgtcgggtg ttcgtcgtcg aagcggcgga
     3661 tgtacttcca gcggtgcgtc cggacgcacc gctggggctc gtaagcgaca tggtaggtct
     3721 tctcggcgaa gatggcgtca cggacttcgt ctgcctcact gttcacgagc ggcaggagcg
     3781 actcgccctg gagccagggg ggtctctccg cgccaacgag gtcacagact gtcgggaaga
     3841 ggtctatgtg cgagacgagg gcgtctatgg ccttcccgcc ggagaaccca cccgggccgc
     3901 gcaggatgag catgacgccc aggccccgat cgtagagggt ggccttcgcg ccggggaagg
     3961 cgaggccgtg gtctgtcgtg cagatgacga gcgtgttctc ggtgagcccc tcggcgtcga
     4021 gcgcgtcgag gacggcgccg atgccgcggt ctagcgagcg ggcgctctcc tggaaagccg
     4081 ccatgtccag gcgcgtctcg ggggtgtcgg gaaggttggg gggaggcatg acgtagttgg
     4141 ccttcttcgg ggaacttggg cggaaccact cgcggtgtgt ctcgaagaat ccgacggaga
     4201 ggaagaaggg cttgtcgctc gcgcgctcca ggaggtcgag ggtcacgggc acgacgtcgt
     4261 cggtgtggtt ggtggggatc ttgtagacct cgtcgtagcc gatgacgtcc ggtctcttgg
     4321 agatgtgttg ctcgccgatc agggctgacc agtagcccag ctcgtgcagg gtgtggac
//
LOCUS       DASTUV010000369         4875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_610858, whole genome shotgun sequence.
ACCESSION   DASTUV010000369 DASTUV010000000
VERSION     DASTUV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610858"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFT03_07415"
     CDS             complement(<1..385)
                     /locus_tag="VFT03_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU4848012.1"
                     /translation="MSQQTPRATSPVAERRRIVIITGLSGAGKSNALQAFEDAGYFCI
                     DNLPPRMIPAVMETVAQGNGGPDGVVVVMDIRGRRYFGAELERSLEAVVEESGWERRI
                     LFIESDDRTLVRRYKESRRPHPAARN"
     gene            803..1711
                     /gene="rapZ"
                     /locus_tag="VFT03_07420"
     CDS             803..1711
                     /gene="rapZ"
                     /locus_tag="VFT03_07420"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU4848013.1"
                     /translation="MSRQTPRATGSPIAESRRIVIITGLSGAGKSNALQAFEDAGYFC
                     IDNLPPRMIPAVMETVAQGNGGPDGVVVVMDIRGRRYFGEELEGSLEAVVEESGWERR
                     ILFIESDDRTLVRRYKESRRPHPAARNGDVLAAIRDERRYLATLREKADLVVDTSGYS
                     ALDARIRFKKLAESFSSRLTVSLISFGFKHGTPLDVDTLFDVRFMPNPHYDPDLRPLT
                     GHDRAVREAVLGHKDAGTFLEKTTELLSFLIPRYAAEGKTYFTLGVGCTGGRHRSVVI
                     VEELARQLHESKAELDLFVRHRDLEI"
     gene            1780..2742
                     /locus_tag="VFT03_07425"
     CDS             1780..2742
                     /locus_tag="VFT03_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEU4848014.1"
                     /translation="MSRYEELRVVAFGGGTGLPVLLRGLKDRVGDLTAVVTVADDGGS
                     SGRLRQELGVAPPGDVRNCLVALAGRKKLAEVFNYRFEGEGDLSDHSVGNLIIAALAD
                     MAGGFCDGVEQAARFLRIKGQVYPAATESLTLVVTHADGTLSRGESVVREVGKAVARV
                     EVEPSSAPAPPGVISAIERADVVVLSPGSLFTSTIPALLGAGVRETLAGFSGPVVYAA
                     NAMTQPGETVGFALSDHLRALESHAGPVVTDVLVHHGGLPKELIARYAAEGAAPVEVD
                     REEVEKLGVRLHRAALLAEDAGAGARHDPDRLAEEVCEAVLVRL"
     gene            2723..3610
                     /gene="whiA"
                     /locus_tag="VFT03_07430"
     CDS             2723..3610
                     /gene="whiA"
                     /locus_tag="VFT03_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU4848015.1"
                     /translation="MSSFASEVRRELASGTTSGSRPTDRSELAGLLDAGGFWDAAGVT
                     FRTQSAAVARSVVRLWRSEFGLESRLSPADTDRFGRARYAVRTEGNGAIQAAEELSFV
                     RVSRSAGDRTAYLRGAFQGAGSVTKPGGRGGYHLEFVHRDEDFIRRVAAFSRAPLRLV
                     RRRGWWVAYTKSADGVTTLLSQMGLHDAVLEYEARAVLGEAKANANRITNFDAANAGR
                     TATAAARQQRALAALDPRGLPPALKEMRELRLEHPDASLTELARLGGLSKSAANHRLR
                     RLVSLAADERHQPTGSRQQ"
     gene            3709..4716
                     /gene="gap"
                     /locus_tag="VFT03_07435"
     CDS             3709..4716
                     /gene="gap"
                     /locus_tag="VFT03_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4848016.1"
                     /translation="MAVRVGINGFGRIGMLTAKAAIERSDDDVQIVAVNDLMPMESLA
                     LLFKRDTVHGIWPEGVELDGNILRVGDREIKTFAERDPAEIPWGDVGADIVVESTGVF
                     TDGEKAAAHIEGGAKKVIISAPAKNVDATFVFKVNHESYDPESDDVVSNASCTTNCII
                     PMVKVLQDNFGVESGYMTTVHAYTNDQSLLDAAHKDPRRARSAPNNIVPTSTGAARTA
                     GVIYPELQGKVDGMSMRIPVPDGSITDFVAQVSQEVSAEEVNDAFRQAADGELSDILE
                     YSDAPIVSSDIVGNPASCVFDSLLTMSNGDTVKVLGWYDNEWGYSNRTVDLAQYIGER
                     L"
BASE COUNT          886 a         1602 c         1590 g          797 t
ORIGIN      
        1 cgttcctggc tgccgggtgc ggcctgcggc tctccttgta gcgcctgacc agcgtcctgt
       61 catcggactc gatgaacagt atgcggcgct cccaaccgct ctcctccacg acggcctcga
      121 ggctccgctc gagctccgct ccgaagtacc gccgcccccg gatgtccatg accacgacca
      181 ccccgtcggg tcccccgttc ccctgcgcga ccgtctccat aacggcgggg atcatgcgcg
      241 gcggcaaatt gtctatgcag aagtagccag cgtcctcgaa ggcctgcaac gcgttcgact
      301 tgcccgcacc cgagaggccg gtgatgatga cgatgcgacg tctttccgcg accggggagg
      361 tagcccgcgg cgtctgctga ctcatgccgc tacactccgc ctctcctgcg gcgtaaagac
      421 catccgttcg cgcagcgtag cactacctcc accagcttgc aacggaaaat gcgtatgatt
      481 gtgagcacgg cgcttcgcgc ctcgtcagca cttcagcacg ctacgctctt ttagggcttc
      541 gcttgtcagc atgctgcctt tcaggcagct tgtcagctag aaggcaagga gggtgcgaca
      601 tggtgcttcc ccgcgctagt ccaaggatat gcccaaagcg tgaccgggtg tgagcccaac
      661 ggcagagaga aagctgaaat gctgacaagc tgcctgaaag gcagcgtgct gaagtgctga
      721 ctccgttgca agctggtaaa ggtagtgcta ggctgcgcga acggatggtc tttacgccgc
      781 aggagaggcg gagcgtagcg acatgagtcg gcagacgccg cgggctactg gctccccgat
      841 cgcggaaagt cgtcgcatcg tcatcatcac cggcctctca ggtgcgggca agtcgaacgc
      901 gttgcaggcc ttcgaggacg ctggctactt ctgcatagac aacttgccac cgcgcatgat
      961 ccccgccgtc atggagacgg tcgcgcaggg gaacggggga cccgacgggg tggtcgtggt
     1021 catggacatc cgggggaggc ggtacttcgg agaggagctc gaggggagcc tcgaggccgt
     1081 cgtggaggag agtggttggg agcgccgcat actgttcatc gagtccgatg acaggacgct
     1141 ggtcaggcgc tacaaggaga gccgcaggcc gcacccggca gccaggaacg gcgacgtgct
     1201 cgctgccata agggacgaga gacggtatct cgcgaccctg cgcgagaagg cggacctggt
     1261 cgtggacacc tccggttact cggcgctgga cgcccgtatc aggttcaaga agcttgccga
     1321 gtcgttctcg agtcgcctca cggtcagcct gatctccttc ggcttcaagc acggcacgcc
     1381 gctcgacgtg gacaccctct tcgacgtgcg gttcatgccg aacccccact acgaccccga
     1441 cctgcggccg ctcacgggcc acgaccgggc ggtgcgcgaa gccgtgctcg ggcacaaaga
     1501 cgccgggacg tttctggaga agacgaccga gctgctctcg ttcctgatcc cgcgctacgc
     1561 cgccgaaggc aaaacgtact ttacactggg cgtcggctgc accggcggcc ggcaccgctc
     1621 ggttgtcata gtcgaagagc tcgccaggca gcttcacgag agcaaagccg agctcgacct
     1681 cttcgtgcgg caccgggatc tcgagatcta gcgtaccggt ccacttagag gtcccagcaa
     1741 cacctgatgc ccgagatctg aactgtatac tgatctgtcg tgagtaggta cgaagagcta
     1801 cgggttgtgg catttggcgg cggaactggt ctgccggtgc tcttgcgggg cctcaaggat
     1861 cgggtaggcg acctcaccgc cgtcgtaacc gtcgcagacg acgggggctc gagcgggcgt
     1921 ctgagacagg agctcggggt ggcgccgcca ggtgacgtga ggaactgcct tgtagccctc
     1981 gctgggcgca agaagctcgc cgaggtcttc aactaccgct tcgaggggga gggtgacctc
     2041 tccgaccaca gcgtcggcaa cctcatcatc gctgcccttg cggacatggc tggggggttc
     2101 tgcgacgggg tggagcaggc ggcccggttc ttgagaataa agggccaggt ctatccggcg
     2161 gcgacagaga gcctcacgct tgtggtcacg catgcagacg gaacgctaag ccgtggcgag
     2221 tcggtcgtgc gcgaggtggg caaggccgtc gcgagggtcg aggtagaacc ttcgagcgcg
     2281 ccggcgccgc cgggtgtgat ctccgccata gagcgggctg acgtggtagt cctgagtccc
     2341 ggcagcctct tcaccagcac catccccgcc ctgctcggcg ccggggttcg cgagacgctc
     2401 gcgggcttct cgggacccgt ggtgtatgcg gctaacgcca tgacccagcc aggcgagacc
     2461 gtgggcttcg cgctctccga ccacctgagg gcgctcgaga gccacgcggg gcctgtggtc
     2521 accgacgttc tcgtccacca cgggggactt ccgaaggagc tcatcgcccg ctacgcggct
     2581 gagggggctg ccccggtaga ggtggaccga gaggaggtcg agaagctcgg cgtccggttg
     2641 cacagggccg ctctgctcgc cgaagacgcc ggggccggag ctcgccacga cccggacagg
     2701 ctggccgagg aggtgtgcga ggctgtcctc gttcgcctct gaggtaaggc gcgagctcgc
     2761 ctccggcacg acctccgggt ccaggccgac cgaccgctcg gagctggccg gtctgctcga
     2821 cgccggcggc ttctgggacg cggcaggggt gaccttccgc acgcagagcg ccgccgtcgc
     2881 gcgctcggtg gttcgcctgt ggcgctccga gttcggcctc gaatcacggc tctcgccagc
     2941 ggacacggac cgcttcggcc gcgcccgcta cgctgtccgc acggagggca acggtgcgat
     3001 ccaggccgcc gaggagctct ctttcgtgcg cgtttcccgc agcgccggcg accgtactgc
     3061 atacctgcgc ggagcgttcc agggcgcggg ctcggtgacg aagccgggcg ggcgcggcgg
     3121 ctatcacctc gagtttgtgc accgcgacga ggactttatc aggcgcgtcg ccgcgttctc
     3181 ccgggccccg ttgcggctcg tgcggcggcg cggatggtgg gtggcgtaca ccaagagcgc
     3241 cgatggggtg acgaccctgc tctcgcagat gggcctccac gatgccgtcc tcgagtacga
     3301 ggcccgcgcc gtcctcggcg aggcgaaggc gaacgccaac cgaataacca acttcgatgc
     3361 ggccaacgca ggccgcaccg cgacagccgc cgcccgccag cagcgcgcac tcgcggccct
     3421 cgacccccga ggactccccc ccgcactcaa agagatgcgc gagctccgac tcgagcaccc
     3481 cgatgcctca ctcacggagc tcgcgcggct cgggggcctc tccaagagcg ccgcgaacca
     3541 ccgcctaaga aggctcgtca gcctggcggc cgacgagagg catcagccca caggctccag
     3601 gcaacagtaa gacctgaaac ctgatgcctg agcactgatt gcttgttaat ttcccgcctt
     3661 ttgggtacaa cagtgagcaa ccaaagcaaa cgacagacag gagaaatcgt ggctgtgagg
     3721 gtaggcataa acgggttcgg tcgtataggg atgctcaccg ccaaggcggc catagagcgt
     3781 agcgacgacg acgtccagat cgtcgctgtc aacgatctga tgccgatgga gagcctcgcg
     3841 ctcctcttca agcgcgatac cgtccacggc atctggcccg agggcgtcga gctagacggc
     3901 aacatcctgc gcgtcggcga ccgcgagata aagaccttcg cggagaggga ccccgccgag
     3961 atcccctggg gcgacgtcgg cgccgacata gtcgtcgagt caacaggcgt gttcaccgac
     4021 ggcgagaagg cagctgccca catcgagggc ggcgcgaaga aggtgatcat ctccgccccc
     4081 gccaagaacg tggacgcaac cttcgtcttc aaggtcaacc acgagagcta cgaccccgaa
     4141 tccgacgacg tcgtctccaa cgcgagctgc accaccaact gcatcatccc tatggtgaag
     4201 gtgctgcagg acaacttcgg cgtcgagtcc ggctacatga ccaccgtgca cgcctacacc
     4261 aacgaccaga gcctgctcga cgcggcgcac aaagacccca ggcgggccag gagcgccccc
     4321 aacaacatag tcccgacctc gacgggcgct gcaagaactg cgggtgtgat ctaccccgag
     4381 ctgcagggca aggtggatgg catgtccatg cgcatccccg tcccggacgg ctcgatcacg
     4441 gacttcgtgg cgcaggtttc gcaagaggtc tcagcagagg aggtcaacga cgctttcagg
     4501 caggctgcgg acggcgagct ctccgacatc ctggagtact ccgatgcgcc gatagtctcc
     4561 agcgacatcg tcggcaaccc ggcaagctgc gtcttcgact ctctcctgac catgtccaac
     4621 ggcgacaccg tcaaggtgct cggctggtac gacaacgagt ggggctactc gaaccgcacc
     4681 gtggacctcg cccagtacat aggcgagagg ctgtagagca cgctgccttt caggcagctt
     4741 gtcagcacgg cgcctttggc gccttgtcag cacgccctct tcgagggctt ctcagctaag
     4801 atggcgcact tcgtcgctcc gcagcgccag acagagttac tgccgcaaag ccgtttcgtc
     4861 atacgtaatc cgggc
//
LOCUS       DASTUV010000370         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1295736, whole genome shotgun sequence.
ACCESSION   DASTUV010000370 DASTUV010000000
VERSION     DASTUV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295736"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..687
                     /locus_tag="VFT03_07440"
     CDS             241..687
                     /locus_tag="VFT03_07440"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU4848017.1"
                     /translation="MRDDGRTLDEAQLAHLAQYFNEEVTFSRHIGAKVERVEPGYSVI
                     LIDVEDYHLNGAGTLHGGVYASLIDNAMGLAVLALVGVRTATIQMNVHFLGAVNEGRI
                     TCESEVLHRTRRTATAEAKVRDAEGNLVALGTGAFRVFEKKGNAIV"
     gene            complement(851..1612)
                     /gene="fabG"
                     /locus_tag="VFT03_07445"
     CDS             complement(851..1612)
                     /gene="fabG"
                     /locus_tag="VFT03_07445"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEU4848018.1"
                     /translation="MGRLEGRVALVTGAGRGIGRATATRFAREGARVCLADVEPNGPA
                     EAASVLLDGGMDAFAARMDVTSRTDVENTVGKTVERYGRIDILVNNAGVTRDNLSHKM
                     TDEDWRKVLNVHLTGAFFCSRAAQRHMVERGYGRIVNVSSTSALGNRGQANYSAAKAG
                     VVGLTKTLAIELGRYGVTVNAVAPGFIETEMTRETAARLGLDFEEFVGEMVKEIPVGR
                     GGWPEDVAAAILYFASEEASFVSGQVLYVAGGPKD"
     gene            complement(1691..1870)
                     /locus_tag="VFT03_07450"
     CDS             complement(1691..1870)
                     /locus_tag="VFT03_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU4848019.1"
                     /translation="METVGVVGTGMMGAGIVQVVAQAAYRVVACDRSVEALERAQLHV
                     RDGLGRKTGGGFYDY"
     gene            complement(1870..>2646)
                     /locus_tag="VFT03_07455"
     CDS             complement(1870..>2646)
                     /locus_tag="VFT03_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU4848020.1"
                     /translation="YFFCDDESVIGAPFYVMERRSGVVVDDEFPEGVEPTRELCQGIS
                     RAVADTLAELHAVESGEAGLGDLGRPEGFLKRQLEGWIGRYEKARTDEIGEVGPLTDW
                     LARGVPESPEPTVIHNDYKLNNLVLNPGDLTEVRAVLDWEMATVGDPLFDLAVSLSYW
                     TEPGDSPELKEVMPTVTSTPGFMTRAEFVERYAEKSGRDLSEMHWYMVFGYFKLAAIL
                     QQIYARWKKGQTKDERFADFDGRVRTLILHANSLAEEGSL"
BASE COUNT          462 a          905 c          793 g          486 t
ORIGIN      
        1 ttcagttgat accatgagca tagccggata accttaccga tagccctgac cgttggccgt
       61 tggagccagt gcttacccac ctacttccag gagacgagtg gaagcaaaag ttctcaagtt
      121 cgtgttccca atcgtgttcc caaccgacac caaccacttt ggggccagct tcggacgtac
      181 gccagcggcc tggatagagc cggcggacag tgacactgta tagatagaag ggagcgttgg
      241 gtgagggacg atggaagaac cctggacgaa gcacagctgg cccatctcgc gcagtacttt
      301 aacgaggagg tcaccttctc caggcacata ggggcaaagg tcgagagggt cgagccagga
      361 tactctgtaa tattgatcga cgtcgaggac taccacctca acggcgctgg caccctgcac
      421 ggtggcgtct acgcctccct catagacaac gccatgggcc tcgctgtgct cgcgctcgtt
      481 ggcgtcagga cggcgaccat ccagatgaac gtacacttcc ttggcgcggt caacgaggga
      541 cgcataacgt gtgagagcga ggtcctccac cgcacccgca ggaccgcaac ggccgaggcg
      601 aaggtccggg atgcggaggg caacctcgtc gcgcttggca cgggggcgtt ccgcgtcttc
      661 gagaaaaagg gcaacgctat agtatgagcc cttccgccac gcctgggagg taagcaccgc
      721 cctcacctgg cagtgccctg aaccgaagct tctggccgcg atgacctcct cgtcgaagca
      781 ccgcgcgagg cgctccagtt gctccgcgtt caacgccctc ccccgtcggc catcccattc
      841 ctcctctcgc tcagtccttc ggtccgcctg ccacgtagag gacctgcccg ctgacgaacg
      901 aggcctcctc cgaggcgaag tacaaaatcg cggcggccac gtcctcgggc cagccgccgc
      961 ggccaaccgg tatctccttg accatctccc ccacgaactc ctcgaagtcc aggccgagac
     1021 gggccgccgt ctcgcgggtc atctccgtct cgataaaccc cggagcgact gcgttcaccg
     1081 tcaccccgta gcgaccaagc tcgatcgcga gcgtcttcgt gaggccgaca acgccagcct
     1141 tggccgccga gtagttggcc tgcccgcgat tgcccagggc gctggtggac gagacgttca
     1201 ctatcctgcc gtatccccgc tcgaccatgt gcctctgcgc cgccctgctg cagaagaagg
     1261 ctcccgtgag gtgtacgttc aagaccttcc gccagtcctc gtccgtcatc ttgtgggaga
     1321 ggttgtccct ggtgacgcct gcgttgttga ccaggatgtc gatccgaccg tagcgctcga
     1381 cggtcttccc gacggtgttc tcgacgtcgg tgcggctggt cacgtccatg cgggccgcga
     1441 aggcgtccat gccgccgtcc aggagcacgc tagcggcctc tgccggtccg ttcggctcca
     1501 cgtccgcgag gcacacgcgg gcaccctccc tggcgaagcg cgtcgccgtg gcgcgaccga
     1561 tgcctcgccc tgccccagtc accagcgcca ccctgccttc gagccttccc atgagcctcc
     1621 tttttcaggt ttcaggtggc ctcagtagcc gaagtctgaa cgtctgcaac cgacagcttt
     1681 tacccgaagc ctaataatca tagaatcccc cgcctgtctt gcggccgagg ccgtctctga
     1741 cgtggagctg cgccctttcg agggcctcga ccgatcggtc gcaggcgaca acccggtagg
     1801 cggcctgagc cacgacctgg acgatgccgg ctcccatcat gcctgtcccc acgacgccga
     1861 ccgtctccat cagaggctgc cctcttcggc gaggctgttg gcgtgcagga tcagggtccg
     1921 cacccgcccg tcgaagtctg cgaagcgctc gtcttttgtc tggcccttct tccagcgggc
     1981 gtagatctgc tgcaggatcg cagccagctt gaagtagccg aagaccatgt accagtgcat
     2041 ctcggagagg tcgcgcccgc tcttctcggc gtagcgctcc acgaactccg cgcgggtcat
     2101 gaagcccggc gtcgaggtaa ccgtcggcat gacctctttg agctccggcg agtcgcccgg
     2161 ctcggtccag tacgagaggg agactgccag gtcgaagagc ggatcgccta ccgtggccat
     2221 ctcccaatcg aggacggcgc gcacttcggt caggtcgccg gggttgagga ccaggttatt
     2281 gagcttgtag tcgttgtgga tgacggtcgg ctcggggctc tcgggaacgc ctctggcgag
     2341 ccagtcggtt agcggcccga cctcccctat ctcgtcggtt ctggctttct cgtagcgccc
     2401 gatccagcct tccaactgcc gcttcagaaa accctcgggg cggccaaggt ccccgaggcc
     2461 ggcctcgcca gactctacgg cgtgcagctc agcgagggtg tcggcgacgg cgcgggagat
     2521 tccctggcac agctcgcggg tgggctcgac cccctcgggg aactcgtcgt ccaccaccac
     2581 gccactgcgg cgctccatta cgtagaaggg cgcccctatg accgactcat catcgcagaa
     2641 gaagta
//
LOCUS       DASTUV010000371         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1166236, whole genome shotgun sequence.
ACCESSION   DASTUV010000371 DASTUV010000000
VERSION     DASTUV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166236"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFT03_07460"
     CDS             complement(<1..572)
                     /locus_tag="VFT03_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU4848021.1"
                     /translation="MESRVTGGNGLVERLLSGDRRALARVISKIERGDPETHEIISEI
                     YPRTGNAPSVGFTGPPGVGKSSIMAKLIELYREEDRRVGVVSVDPSSPFSRGAILGDR
                     IRLSDHFLDPGVFIRSMGSRGHLGGLAGASRLAALAMEAAGMDVVLFETVGVGQGEVE
                     VASAADTVVLALQPGTGDAVQALKAGVMEIA"
     gene            complement(852..1322)
                     /locus_tag="VFT03_07465"
     CDS             complement(852..1322)
                     /locus_tag="VFT03_07465"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU4848022.1"
                     /translation="MKEKYRGEPGSSRITLRARGSQTDTPISCSVDIGRAVYEAQAHA
                     GVGGAGTAACSGDLLLGALAACAQVTCQMVAEAMGIEAERIEVDVEGELDLAGTLGVS
                     REVPVGFERIRTRFDVVAPEATEEQLDDLRRKTEQYCVVFQTLSQPPELETEWA"
     gene            complement(1443..2741)
                     /gene="odhB"
                     /locus_tag="VFT03_07470"
     CDS             complement(1443..2741)
                     /gene="odhB"
                     /locus_tag="VFT03_07470"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEU4848023.1"
                     /translation="MAVEIHVPELGESVADATVGRWLKQEGEAVKSGEALVELETDKI
                     NFEVEAEQDGVLESISKAEGETVGVGEVIGTIGEGEADGAGSDAPAEEPEEEEEEEAP
                     QEDRAETESQDTADEDGHREVEHEVRASPSVRKLAQEYDIDLGEVSGSGSRGRVTRED
                     VERLIREQSSGTTTTREAERPAAPEEDGKREAAPAPAAEDGREERVRLSRRRLTIAQR
                     LVESQQNSAMLTTFNEVDMSAVMELRRRRKDAFQERTGARLGFMSFFTKASIAALKAF
                     PKVNAELQDNELVLKHYYDVGVAVSTDEGLVVPVVRDADRKNFAEIEQTIADLATRAR
                     EGKLTLEDLRGGTFTITNGGIFGSLLSTPILTLPQVAILGMHKIQDRPVAVDGEVEIR
                     PMMYIALSYDHRVIDGAEAVSFLVRIKELIEDPESLLLEG"
     gene            complement(2781..>3922)
                     /locus_tag="VFT03_07475"
     CDS             complement(2781..>3922)
                     /locus_tag="VFT03_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU4848024.1"
                     /translation="KTRGELDDEEGIDWAHAEELAFASLLEDGVPIRLTGQDTVRGTF
                     SQRHAAIYDVETGKRYVPLQNIPQANASFAVYNSPLSEIAVIGFEYGYSVNAHDALVL
                     WEAQYGDFANVGQPMIDQFIVSGQAKWGQTSGLVLLLPHGYEGQGPEHSSARLERFLQ
                     LAGHENIRVANLTTAAQYFHLLRAQAALEDNKRPLIIMTPKSLLRHPMAASDPAELTE
                     GGFLPVLDDEEARKRADSVERLILCSGKIHTELAGSDLREEAVDTAVARIELLYPFPE
                     EQVRELIEGYPNLREILWVQEEPKNMGAWTFMEPRLRALTDGEFPVRYVGKPARPSPA
                     QGSARFHKEEHAGIVRAAFESVEGDLEEAETAAQGTQGRAPSKSD"
BASE COUNT          574 a         1377 c         1209 g          762 t
ORIGIN      
        1 gctatctcca tgacccccgc cttcaaagct tgcaccgcgt ccccggtccc cggctgcaag
       61 gccagcacca ccgtgtccgc agccgacgcc acctcgacct caccctgccc caccccgacc
      121 gtctcgaaca ggaccacgtc catccctgcg gcctccatcg cgagggcggc gagccgcgag
      181 gctcccgcca gtccccccag gtgtccccgg ctccccatcg agcggataaa gacgccgggg
      241 tcgaggaagt ggtcggagag ccggatgcgg tccccgagta tggcgcctct ggagaaaggg
      301 ctcgaagggt cgaccgagac gacccccacc ctcctgtctt cctcgcggta gagctcaatg
      361 agcttcgcca tgatgctgct cttgcccacc cctggcgggc ccgtgaagcc gacgctagga
      421 gcattgcccg ttcttgggta gatctcggag ataatctcgt gcgtctcggg gtccccgcgc
      481 tcgatcttcg agatcacacg cgcaagagcc cgccggtcac cggagagcaa ccgctcgacc
      541 aggccatttc cgccggtcac cctactctcc acacacgtac tctggcttcc atacaggaag
      601 ccagagtacg atacgcctcg cgctccgaca aggcagggat accgcgaacc ttcctcggtc
      661 acatgctctg cacctgtgtt gctctgcctt gcgtggcaaa tctggcgagc atcatgttca
      721 tctgcatacc tcgctggcgg ggaggatctg agaatagcgg cacgatctta ccgcttacga
      781 cgcggcctac gtcgcgctcg ccgaggcgct agacgcgccg cttgtcacga cgccactttg
      841 gagttgcacc ttcaagccca ctccgtctcc agctccggcg gctggctcag cgtctggaag
      901 accacgcagt actgctccgt cttgcgccgc aggtcgtcca gttgctcctc cgtagcctcg
      961 ggcgctacga cgtcgaacct ggtgcgtatg cgctcgaagc cgacgggcac ttccctggag
     1021 acgcccagag ttcccgcgag gtccagctcg ccttcgacat ctacctcgat gcgctcggcc
     1081 tctatgccca tggcctcggc taccatctgg caggttacct gggcgcaggc cgccagagcg
     1141 ccgagcagga ggtctcctga gcaggcagcc gtgcctgccc ctccgacgcc tgcatgggcc
     1201 tgggcctcgt agacggcgcg gcctatgtcc accgagcagg agatcggggt gtctgtctgg
     1261 ctacctctcg cccggagcgt tatacgagaa gagccgggtt cgccgcgata cttttctttc
     1321 aacggccttt gcaccgatct gagatccatg gccctcccat cagtggctgc ctttccgccg
     1381 cgaattgtac cgcgaggggg tcctcctcgg aggaccccct ctgcgccgtt atctggtcgg
     1441 tactaaccct ccagcaggag agactccggg tcttcgatca gctcctttat tcgcacgagg
     1501 aagctcacgg cctcggcgcc gtctatgacg cggtggtcgt aggagagtgc aatgtacatc
     1561 atcggtcgga tctcgacctc tccgtcaacc gcaaccgggc ggtcctggat cttgtgcatc
     1621 cccaggatgg ctacctgcgg cagcgtcaat ataggtgtcg agagcagcga gccgaagatg
     1681 ccgccgttgg tgatggtaaa cgtgccgccg cgcaggtcct ctagcgtgag cttgccctcg
     1741 cgagctctgg tcgcgaggtc ggctatggtc tgctcgatct cggcgaagtt cttgcggtcg
     1801 gcgtcgcgca ccacggggac gacgaggccc tcgtcggtgc tgaccgccac ccctacgtcg
     1861 tagtagtgct tgagcacaag ctcgttgtcc tgcaactcgg cgttgacctt cgggaacgcc
     1921 ttgagcgccg ctatggaagc cttggtgaag aagctcatga agcccaggcg cgcgcctgtg
     1981 cgctcctgga aggcgtcctt gcgccgccgc cgcagctcca tgacggcgct catgtccacc
     2041 tcgttgaagg tggtgagcat cgcggagttc tgctgcgact cgacgagtcg ctgggcgatg
     2101 gtgagcctgc ggcgcgaaag gcgcacccgc tcctcgcggc cgtcctcggc cgcaggggct
     2161 ggcgccgctt cccgcttgcc gtcttcctcg ggggctgcgg gccgctcggc ctcgcgggtg
     2221 gtagtggtgc cgctactctg ctcgcggatc agacgctcca cgtcttcgcg cgtaaccctc
     2281 ccgcgcgagc cgctccccga gacctctccg aggtcgatat cgtactcctg ggccagcttc
     2341 ctgacggagg gcgaggccct gacctcgtgc tcgacctccc tgtggccgtc ctcgtcagcg
     2401 gtgtcctggg actcggtctc cgcccgatcc tcttgtgggg cttcctcttc ttcctcttcc
     2461 tcgggttctt cggcgggagc atcgctgccg gcgccatcgg cttcgccttc tccgatggtc
     2521 cctataacct ctccgacgcc aacggtctcg ccctcggcct tggatatgga ctccaggacg
     2581 ccgtcctgct cggcctcgac ctcgaagttg atcttgtcgg tctcgagctc gacgagggcc
     2641 tcgccggact tcacggcctc gccctcctgc ttgagccagc gcccgaccgt cgcgtcggct
     2701 accgactccc cgagctccgg gacgtgtatc tcaaccgcca actggcaaca cctccttctc
     2761 taggatctcc acccggagct tcagtcgctc ttgctcggag cgcggccctg cgtcccctgc
     2821 gcggccgtct cggcctcctc caggtcccct tcgacgctct cgaaggctgc ccggactatg
     2881 cccgcatgct cctccttgtg gaagcgagcg gagccctggg cagggctggg ccgggccggc
     2941 ttgccgacgt accgaacagg gaactccccg tcggtcagcg ctcgcaggcg cggctccatg
     3001 aacgtccagg ctcccatgtt cttcggctcc tcctgaaccc agagtatctc gcgcaggttg
     3061 gggtagccct cgatgagctc ccgcacctgc tcctccggga aggggtagag tagctctatc
     3121 cgggccaccg ctgtgtccac ggcctcctcc ctgaggtcgc tgccggcgag ctccgtgtgg
     3181 atcttgcccg agcacaggat gagccgctcg accgagtcag ctctcttgcg agcctcctca
     3241 tcgtcgagca cggggaggaa gccgccttct gttaactccg cagggtcgga ggctgccata
     3301 gggtgcctga ggaggctctt cggggtcatt attatgaggg gccgcttgtt gtcctcgagc
     3361 gcggcctggg cgcgcagcag gtggaagtac tgggccgccg tggtgaggtt ggccacccgg
     3421 atgttctcgt gtcccgcgag ctgcaagaag cgctccaggc gggcgctcga gtgctcggga
     3481 ccctggccct cgtagccgtg cggcaggaga agtacgagcc ccgaggtctg gccccatttg
     3541 gcctgcccgg agactatgaa ctggtcgatc atgggctgcc cgacgttggc gaagtcgccg
     3601 tactgggcct cccacaggac cagagcatcg tgcgcgttca ccgagtagcc gtactcgaag
     3661 cctatgacgg cgatctccga gagggggctg ttatataccg cgaacgaggc gttcgcctgc
     3721 gggatgttct ggagcggcac gtagcgcttc ccggtctcta cgtcgtagat ggccgcgtgc
     3781 cgctgggaga acgtgccccg cacggtgtcc tgcccggtga gccggatcgg cacgccgtcc
     3841 tcgagcagcg aggcgaaggc gagctcctcg gcgtgcgccc agtctatgcc ctcctcgtcg
     3901 tccagctcgc cgcgggtctt ct
//
LOCUS       DASTUV010000372         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1626697, whole genome shotgun sequence.
ACCESSION   DASTUV010000372 DASTUV010000000
VERSION     DASTUV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626697"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFT03_07480"
     CDS             complement(<1..985)
                     /locus_tag="VFT03_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU4848025.1"
                     /translation="MEPEKDLVSGIAQEAGVDPLCARVLANRGVLPEGALGFLSPSLK
                     TLRAPEEEVWRVAARRVARAVRDGERIGVFGDYDADGITSAAVLYLALSHYTDNVVVQ
                     LPIRQVGYSLLEPYVRDLFAEGVDLLVTADCGISNRSEVALANELGMEVVVTDHHIPP
                     EDPPDTAVAVLDPKLWNPEDPLAGVGVAWKFAWAVARRLGDAEGRKRLGRLLDLVSVG
                     TVVDIAPLVGDNRALARMGLKHINRGLAAGEARPGIEALVKVAGVRGELDEDDLGWKL
                     GPRINSIGRIKNPRPALDLLLTDDRREAFRIASLLNQLNAERQRRTRFAVER"
     gene            complement(1102..2346)
                     /locus_tag="VFT03_07485"
     CDS             complement(1102..2346)
                     /locus_tag="VFT03_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU4848026.1"
                     /translation="MFTFSNLRKLGLRAVAALTALQVFLVGVLVAMAELRKRRQGPRE
                     GFPWVDQPEVELESGDSRLKVYAYGVPLYEAMLAEIDSAEERILVGTFIWKGDEVGRR
                     FVEALARKAREGVIVCVIFDGLANVVVPPSFKRFPEEIHTLHFRPLWGPENLANPRNV
                     FRYHKHLMAVDGRVAFLGGYNIGSLYAAGWRDTHVRVTGPGAREVENDFVDFWNAHRL
                     ADLPELEAAGERSWDPAAVFHRNDPYMRIFPIRAMYLEAIDRANEHVYLTHAYFIPDR
                     AMRIRLVEAARRGVDVQVLVPELSNHVTADWLGRRHFHELLSAGVRIFRYKHIMIHSK
                     TATIDGVWSTVGSANIDRYSLLGNYEINLEVYSRRFAARMERMFELDKTNASELTLEE
                     WERRPLPAKVVERVLATLSPLV"
     gene            complement(2399..3148)
                     /locus_tag="VFT03_07490"
     CDS             complement(2399..3148)
                     /locus_tag="VFT03_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HEU4848027.1"
                     /translation="MGLRNLYKVFNETRKAAREQATLAVVGDSPRASEVAGLLGAQRS
                     VRGAEVILSVSGETITLSGKAVEEPGEIPLPSRDSDAGLEELTSRIVRALDEDYLVAL
                     AKGYPQFRRAVCEEFVRHNARQNAVIGALPIPGADMPVMTANQARMVLSIAAAYGEDL
                     SLERARELLGVLAAGFGMRALSRQAVKLVPFAGWAAAAAIGYAGTVAMGRATMLYFER
                     GGQRVGEKEMAEIQRRAVEEGKAFASRLRRR"
     gene            3275..>4212
                     /locus_tag="VFT03_07495"
     CDS             3275..>4212
                     /locus_tag="VFT03_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4848028.1"
                     /translation="MQPQRDLRDIEEIQELFETGQETGTLESSEVLDLLQEVDLSTDE
                     IQQVYGLLREHGVEIVDTGFLAETLNEEEDIQLVEEVLEGDLKSRYTGDAVQMYLDEI
                     GKTPLLTKTQEVYLARRIERGDRRAKAHLTKANLRLVVSIAKKYAGRGVSLLDLIQEG
                     NIGLMRAVEKFDYRKGFKFSTYATWWIRQAVTRAIADKGRTIRVPVHMVEKINKYYRI
                     QRALAAELNRDPTDEEVARLMEVETEEIVRIRRVSRRSISLETPVGEDHSSELGDFIA
                     DEESESPHDLAKSSLLKARMREALNGLPERERMVLE"
BASE COUNT          785 a         1444 c         1285 g          698 t
ORIGIN      
        1 cccgctcgac ggcaaacctg gtccgccgct gccgctcggc gttgagctgg ttcaggaggg
       61 aagcgatcct gaaagcctcc cgacgatcat cggtcagaag caggtcgaga gcaggccgcg
      121 ggttctttat cctgccgatg gagttgatgc gcggcccgag cttccacccc aggtcgtcct
      181 catcgagctc ccccctcacc cctgccacct tgaccagcgc ctcgataccg ggacgcgcct
      241 ctccggcagc gaggcccctg tttatgtgct tgagacccat gcgggcgagc gccctgttgt
      301 ccccgacgag cggcgcgatg tcgacgacgg taccgaccga gacgagatcc agaagcctcc
      361 ccagacgttt cctgccctcg gcgtcgccga gcctgcgggc gacggcccag gcgaacttcc
      421 aggcaacccc caccccggcc agagggtcct caggattcca gagcttggga tcgagcacag
      481 cgactgccgt atcgggcggg tcttcaggcg ggatgtgatg gtccgtcacg accacctcca
      541 tccccagctc gttcgccagc gccacctcgc tcctgttgga gatgccgcag tcggcggtca
      601 ccaggagatc aacaccctcg gcgaacaggt cgcgcacgta gggctccagc aggctgtacc
      661 ccacctgccg gatcgggagt tgcacgacca cgttgtccgt gtagtgcgag agcgcgaggt
      721 agagcacggc ggccgaggtg atgccgtctg cgtcgtagtc cccgaagacg cctatgcgct
      781 ccccgtccct gaccgcccgc gcgacccggc gggcagccac tcgccacacc tcctcctctg
      841 gcgcgcgcag ggtcttcagc gacggcgaga ggaagcccag agcgccctcg ggcaagacgc
      901 cccggttcgc tagcacacgg gcgcacagcg ggtcaacccc cgcctcctga gcgatcccgg
      961 aaacgagatc cttctccggc tccataagcc gccaccgcgc caaacaagcc ctccccgacg
     1021 caaacagact atgcttcgtc attatacagc ggacaccggt gtaatcggac cggtcgcaac
     1081 agcgctaaac tcggctcacg ctcagacgag cgggcttagg gtggccagta cccgctcgac
     1141 aaccttggcc gggaggggac gcctctccca ctcttcgagc gtgagctcgc tagcgttggt
     1201 cttgtccagc tcgaacatcc gctccatccg ggcggcgaag cgtctcgagt agacctcgag
     1261 gtttatctcg tagttcccca gcaagctgta gcggtctatg ttcgcagagc cgaccgtgga
     1321 ccagacgcca tctattgtgg ccgtcttgga gtggatcatg atgtgtttgt agcggaagat
     1381 cctcacgccc gcggagagca actcgtggaa gtggcgcctt ccgagccagt ccgcagtgac
     1441 gtggttggag agttccggca cgaggacctg tacgtccacg ccacgccttg ccgcctcgac
     1501 gaggcggatg cgcatagcac ggtcggggat gaagtaggcg tgggtgaggt aaacgtgctc
     1561 gttcgcccgg tctatggcct cgaggtacat cgcccggatc gggaagatcc tcatatacgg
     1621 gtcgttgcgg tggaaaacag ccgccgggtc ccacgagcgc tctcctgccg cctccagctc
     1681 cggcaggtcc gccaggcggt gggcgttcca gaagtccacg aagtcgtttt cgacctcgcg
     1741 ggcgcccggg cccgtgaccc tgacgtgggt atcgcgccag ccagccgcgt agagggagcc
     1801 gatgttatag ccgccgagga aggcgacccg cccatccact gccataaggt gtttgtgata
     1861 cctgaagacg ttgcgcgggt tcgcgaggtt ctccggcccc cacagcggcc ggaagtggag
     1921 cgtatgtatc tcctcgggga accgcttgaa cgaaggaggg acgacgacgt tcgcaaggcc
     1981 gtcgaagatc acgcacacga tcaccccctc gcgggccttc ctcgccagcg cctccacgaa
     2041 tcggcgcccg acctcatcac ccttccagat gaacgtgccg accaagatcc tctcctcggc
     2101 gctatcgatc tccgcgagca tcgcctcgta gagagggacg ccgtaggcgt agaccttgag
     2161 ccggctatct cccgactcca gctctacctc gggctggtca acccacggga acccctcgcg
     2221 cggcccctgc cgacgcttcc tcagctcggc catcgccacc agcacgccga caagaaagac
     2281 ctgcaacgcc gtgagcgccg cgacggccct gaggcccagc ttccgcaggt tcgagaacgt
     2341 gaacatgcag acgattatag aagcgtagag gggctgctcg cgtctccgcc cctacgctct
     2401 agcggcggcg gagacgcgag gcgaaggctt tgccctcctc gacggcgcgg cgctggatct
     2461 cggccatctc tttctcgccg accctctggc ctccgcgctc gaagtagagc atggtcgccc
     2521 gacccatcgc gacggttccg gcgtagccga tggccgctgc ggcagcccag cccgcgaagg
     2581 gcaccagctt cacggcctgc cgggacagcg cccgcatccc gaagcccgca gccagcaccc
     2641 caagcagctc ccgcgccctc tctagcgaga ggtcctcacc gtaggcggcg gcgatgctca
     2701 gaaccatcct ggcctggttc gcggtcatga ccggcatgtc cgcaccaggg atcggcaacg
     2761 ccccgatcac ggcgttctgg cgtgcgttgt ggcgtacgaa ctcctcgcag accgcccgac
     2821 gaaactgcgg ataacccttc gcgagcgcga ctaggtagtc ctcgtctaac gcccgcacga
     2881 tccggctggt caactcttcg agcccggcgt cgctatccct tgagggcagc gggatctctc
     2941 ccggctcctc gactgccttg cccgagagcg taatcgtctc ccccgagacg gagaggatta
     3001 cctcagctcc cctaacgctc ctctgcgcac cgaggaggcc cgcgacctcg gatgcccggg
     3061 gcgagtctcc gacgacggcc agagtcgcct gctccctggc agctttgcgc gtttcgttga
     3121 acaccttgta gaggttcctg aggcccatca aagctcgact tcctcctgct cttccggctg
     3181 caagccggca tgttaaattg attcctgatc tttttcagat atattacgtg caggcaacag
     3241 aagggtttca gacctggata accctgagga aagcttgcag ccccagcgcg acctgcggga
     3301 catagaggag atacaggaac tcttcgagac tggccaggag acaggcaccc tggagtcaag
     3361 cgaggttcta gatctcctgc aggaggtcga tctctcgacc gacgagatcc agcaggtcta
     3421 tggcctcttg cgcgagcacg gcgtcgagat cgtggacacg ggctttctcg ccgagaccct
     3481 caacgaggag gaggacatac agctcgtcga ggaagtcctc gaaggcgacc tcaagagccg
     3541 ttacacagga gacgctgtcc agatgtacct ggacgagatc ggcaagaccc ccctcctcac
     3601 caagacccag gaggtctacc tggcgagacg catcgagcgc ggggacaggc gagcgaaggc
     3661 gcacctcacg aaggcgaacc tgaggctcgt tgtctccata gccaagaagt acgccggcag
     3721 gggcgtctcg ctcctcgacc tcatccagga ggggaacata gggctcatgc gggcagtcga
     3781 gaagttcgac taccgcaagg gcttcaagtt ctccacctac gctacctggt ggataagaca
     3841 ggccgtcacc cgcgccatcg cggacaaggg gcgcacgatc cgggtccccg tccacatggt
     3901 cgagaagatt aacaagtact acaggatcca gcgcgccctg gccgcagagc tgaaccgcga
     3961 cccgacggac gaggaggtcg cccgcctcat ggaggtggag accgaggaga tagtgcgcat
     4021 ccggcgcgtc agccggcgtt ctatctcgct cgagaccccc gtaggcgagg accactcctc
     4081 cgagctcggc gactttatcg cggacgagga gtccgagagc ccacacgacc tggcgaagtc
     4141 ctcgctgctg aaggcccgca tgcgcgaggc ccttaacggc cttcccgagc gcgagcggat
     4201 ggtcctcgag ta
//
LOCUS       DASTUV010000373         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_432855, whole genome shotgun sequence.
ACCESSION   DASTUV010000373 DASTUV010000000
VERSION     DASTUV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432855"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFT03_07500"
     CDS             <1..222
                     /locus_tag="VFT03_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4848029.1"
                     /translation="QILAAELEERGIRANAVDPGGMRTGMRAAAYPEEDPLTRITPEE
                     NTDIFLYLASDESRAVTGQRFKAQEFQRA"
     gene            complement(229..864)
                     /locus_tag="VFT03_07505"
     CDS             complement(229..864)
                     /locus_tag="VFT03_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEU4848030.1"
                     /translation="MKKARTTNIISWVLSLIMIAAGAGLIATFFLAGSLNSAATNSSD
                     PGGFNVPRLDTDDHAVTSGPKNKMLKLTIPEMNQIKDDTIPTVSGTDMQTLGNHAAIH
                     LAGTGFPWEDEANVYMAGHRLGYPRTPSFLAFFDLNKLEQGDEIYVTDANGTKYTYSV
                     FKEFTVSPTDLSVIDTVPGKNVLTLQTCTLPDYSQRLIVQAELIDATQAAA"
     gene            complement(913..1791)
                     /locus_tag="VFT03_07510"
     CDS             complement(913..1791)
                     /locus_tag="VFT03_07510"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU4848031.1"
                     /translation="MESFDPLVTTDRLAGHLEDPGVRIVDIRGYVNKTDLGGGRQRAE
                     YVGARDEYDEAHVPGAVYVDWTCDITDPDAAVSVQVAPPERFAQAMGSMGIGDETQVV
                     VYDHAGGQFATRLWWALTYYGHDRVSVLDGGWNRWTAEGRPTTPEVPEPETATFTPEP
                     RPAWRKEAEEVLAASERGSAFILDARDEAQYTGAIARGEGRAGRVPGAKHLHSDSLFD
                     PQKGTFRSDEELAGMVREAGVPDDRDAPLVAYCNGGVAATVPLFVLHRLGHTNLAVYD
                     GSWNEWGMREDLPAER"
     gene            complement(1796..3349)
                     /locus_tag="VFT03_07515"
     CDS             complement(1796..3349)
                     /locus_tag="VFT03_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4848032.1"
                     /translation="MLADVKKLDVPDPLELLGKAFREAGHELYLVGGYVRDALLGRED
                     SKAHPDATTDARPAEIRHILSPHAEHFWTVGERFGTIVAQVGGIEIDVTTYRSDLYTE
                     GSRHPEVTFGDSLEEDLARRDFTINAIAADTLTGEILDPFEGREDLERGVIGPVGDPL
                     DRMRDDPLRMLRAVRFEATLSTPEKPFAMTPDLEGAIKENAHWLESISAERIRDEFEK
                     ILRSENVARGLRTLVRLDLMRYIVPEFMETVNVEQEAEFHHKDVFEHTLIVVSSIERE
                     PGMSEWEFSILRKAAFFHDIGKPRTLVYEHRCSYCGAKSLHESAEEGECERCGGRTVP
                     KQIHFYGHENVGAAIARRAMRRLAYPKDDIDAVAHLVANHMRPYGYAASRDPWSDSAI
                     RRFIRDAYLARGDRELSHVDMLLKLARADITGSAPRRRRIAEESWRSLRSRVDEIRAE
                     DAVEKLESPLDGNELMRQFGRGPGRWIKTLKDHLQDEVVEGRLGKDDREKARELAEAY
                     AREHHLFEE"
     gene            complement(3403..3738)
                     /locus_tag="VFT03_07520"
     CDS             complement(3403..3738)
                     /locus_tag="VFT03_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4848033.1"
                     /translation="MDFEVSIDEHAEAYSVISVRGEVDLHTAPKVQYAIERAASTDGA
                     VVLDMAGVAFMDSTALSALVRAKDALHEQGISLRLAAPSKPVERIFSVTGFRDYFEIF
                     PSREAAIAG"
BASE COUNT          653 a         1268 c         1268 g          775 t
ORIGIN      
        1 cagatcctgg ccgcagagtt agaggagcgt ggcatccggg cgaacgccgt ggaccccggt
       61 gggatgcgca cgggaatgcg cgccgccgcc taccccgaag aagatccctt gacgcgtata
      121 accccggaag agaacaccga catcttcctt taccttgcct cggacgagtc gagagccgtg
      181 acagggcagc gcttcaaggc gcaggagttt cagagggctt aagagacctc aggcggcggc
      241 ctgggtcgcg tcgatcagct cggcctggac tatgaggcgc tgcgagtagt cgggcagggt
      301 gcaagtctgg agggtgagaa cgttcttgcc cgggacagtg tcgatcactg agaggtccgt
      361 agggctcacg gtgaactcct tgaatacact gtaagtgtac ttcgtcccgt tggcgtcggt
      421 gacgtagatc tcatcaccct gctcgagctt gttgaggtcg aagaaggcga ggaagctggg
      481 ggtacggggg tagccgaggc ggtggccagc catgtagacg ttcgcctcgt cttcccacgg
      541 aaagcccgtg ccggcgaggt ggatcgcggc gtgattcccg agggtctgca tgtccgtgcc
      601 ggacacggtc gggatggtgt cgtccttgat ctggttcatc tccgggatcg tcagcttcag
      661 cattttgttc ttcggcccac tcgtcacggc gtgatcatcc gtatcgagcc tggggacgtt
      721 gaaaccgcca gggtcggagc tgttcgtggc tgcgctgttg aggctaccgg ccaggaaaaa
      781 ggtggctatg agcccggcgc cggccgctat catgatgagg ctcaacaccc aggagatgat
      841 gttagtggtt ctcgccttct tcataaaacc tcaaagattg taacaccagg gttatgcgcc
      901 ttcacggata ccctagcgtt cggcgggcag gtcctcgcgc ataccccact cgttccagga
      961 accgtcatag acggccaggt tagtgtggcc gagccggtgg aggacgaaga gcggcaccgt
     1021 agcagccacc ccgccgttgc agtaggctac caggggtgcg tccctgtcgt cggggacgcc
     1081 ggcctcccgc accatcccgg ccagctcctc atccgagcgg aacgtgccct tctgcgggtc
     1141 gaagaggcta tcggagtgga ggtgcttcgc tccagggacg cggccagccc gcccctctcc
     1201 ccttgctatg gcgccggtgt actgcgcctc gtcgcgcgcg tcgaggatga aagcgctgcc
     1261 gcgctcgctc gcggctagca cctcctcagc ctccttgcgc cacgcgggac gcggctccgg
     1321 ggtgaaagtg gctgtctctg gttccggaac ctcgggggtc gtcgggcggc cctcggcggt
     1381 ccacctgttc cagccgccgt cgaggacgga gacgcggtcg tggccgtagt aggtcagcgc
     1441 ccaccagagg cgcgttgcga actggccgcc ggcgtggtcg tagacgacga cttgtgtctc
     1501 gtccccgata cccatagagc ccatagcttg cgcgaagcgc tccggcgggg cgacctgcac
     1561 ggagacggct gcgtcggggt cggttatgtc gcaggtccag tccacgtaga ccgcgccagg
     1621 tacgtgggcc tcgtcgtact cgtcgcgggc ccccacgtac tcggccctct ggcggccgcc
     1681 gccgaggtcc gtcttgttta cgtagcccct gatgtccacg atccgtacgc cgggatcttc
     1741 gagatgcccg gcgagccggt cggtggtaac gaggggatcg aagctctcca tagctttact
     1801 cctcgaaaag gtggtgttcg cgggcgtagg cctcggcgag ctcgcgggcc ttctccctgt
     1861 cgtccttacc gaggcggccc tctacgacct catcctgaag gtggtctttg agggtcttga
     1921 tccagcgccc cggcccgcgc ccgaactgcc gcatgagctc gttgccatcc agcgggctct
     1981 cgagcttctc cacggcgtcc tcggcccgga tctcatccac cctgcttctc aggctgcgcc
     2041 aggactcctc ggcgatgcgc ctccgcctcg gggcgctccc tgttatgtcc gctctagcca
     2101 gcttgaggag catgtccacg tgcgaaagct ccctatcgcc gcgcgcgagg tacgcgtccc
     2161 ggatgaagcg cctgatcgcg gagtcgctcc acgggtcgcg gctcgcagcg tacccgtaag
     2221 ggcgcatgtg gttggcgacg aggtgggcta cggcgtctat gtcatccttc gggtaggcaa
     2281 gccggcgcat cgcccggcgc gctatggcgg ccccgacgtt ctcgtggccg tagaagtgga
     2341 tctgcttcgg cacggtcctg ccgccgcagc gctcgcactc cccctcctcg gcgctctcgt
     2401 gcaggctctt cgcgccacag tacgagcagc ggtgctcgta gacgagggtg cgcggcttgc
     2461 caatgtcgtg gaaaaaggca gccttgcgaa ggatggagaa ctcccactcg ctcatgccgg
     2521 gttcacgctc gatgctcgag accactatca gggtgtgctc gaagacgtcc ttgtggtgga
     2581 actcggcctc ctgctcgacg ttcaccgtct ccatgaactc cggcacgatg tagcgcatga
     2641 ggtcgaggcg caccagggtc ctcagacccc gggcgacgtt ctccgagcgg aggatcttct
     2701 cgaactcatc cctgatacgc tcggcgctta tgctttcgag ccagtgcgcg ttctccttga
     2761 tggcgccctc gagatcaggc gtcatggcga agggcttctc aggggtgctc agggtcgcct
     2821 cgaagcgtac cgcccgcagc atccgcaagg ggtcgtcgcg catccggtcc agcgggtccc
     2881 cgacgggacc gatcacaccc ctctccaggt cctcgcgccc ctcgaagggg tcgaggatct
     2941 caccggtcaa agtatccgcc gctatggcgt ttatcgtgaa gtccctgcga gccaggtctt
     3001 cctccagact atccccgaag gtcacttcgg ggtgccggct accctccgta taaaggtcgc
     3061 tgcggtacgt cgtgacgtct atctcgatac cgcctacctg agcgacgatc gtgccgaacc
     3121 gctcccccac cgtccagaag tgctccgcat gggggctcaa gatatgcctg atctccgccg
     3181 gcctggcgtc cgtcgtcgca tcgggatgcg ccttcgagtc ctctctaccg agaagagcat
     3241 cccgcacgta cccacctacc agatacagtt cgtgccccgc ctcccggaac gccttgccca
     3301 ggagctccag gggatcgggc acgtccagct ttttgacgtc tgccagcatc actcaagaga
     3361 ttctagtacg cgagcccacc gcgtccagag gggcggtgcc gcctatcctg caatggcggc
     3421 ttcgcgggag gggaagatct cgaagtagtc tctgaagccg gttacgctga agatgcgctc
     3481 gactggcttt gagggagcgg cgaggcgcag tgagatcccc tgctcgtgca aggcatcctt
     3541 ggcgcgcacg agggcagaga gcgcggtcga gtccatgaac gcaacaccgg ccatgtcgag
     3601 gaccacggcg ccgtccgtgc tcgcggcccg ctctatggcg tactggacct tcggggccgt
     3661 gtggaggtcg acctcgccgc gcaccgagat gacggagtag gcttcggcgt gttcgtctat
     3721 gctgacctcg aagtccatgc cggtcctgac tcctctctac aaagccgcgt aaccctcgga
     3781 tattaaccca ggttccgcag ccggtaaacc gcttgtttta tggggttata gcgcgttttc
     3841 tgttaacatc caggtaatgg agaagagaga gaatgtagag cgggttgcgg tcttcgtcga
     3901 cggggcgaac ctctatcatt cgatcaagaa ttattacaag ggcgtcctcg actacggccg
     3961 tcta
//
LOCUS       DASTUV010000374         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1666188, whole genome shotgun sequence.
ACCESSION   DASTUV010000374 DASTUV010000000
VERSION     DASTUV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666188"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..839)
                     /locus_tag="VFT03_07525"
     CDS             complement(180..839)
                     /locus_tag="VFT03_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEU4848034.1"
                     /translation="MLYGGPPFEDREDAGRRLAERLTRYRDERPVVFALPRGGVPVGY
                     EISRSLGAPLDVFVSRKLGAPGQPEFGIGAVAPGGVRVLNGNVVERLGIPDDYLEAVT
                     RKELAEVERRLRHFRGDRPEPEVRDRTVILVDDGLATGVTARAAVEALRRLGPRRLIL
                     AAPVCAAQTAGLISPEVDELVCLETPSDLGAIGFWYRDFSQTSDEEVIELLESARQGE
                     V"
     gene            complement(878..2308)
                     /locus_tag="VFT03_07530"
     CDS             complement(878..2308)
                     /locus_tag="VFT03_07530"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEU4848035.1"
                     /translation="MNFERLGQYRYRIPREGKMRVEAAFYASEKILSDLAAEDYASLR
                     QLQNVATLPGIVEPALSMPDIHWGYGFPIGGVAAFDPQEGGVVSPGGIGFDINCGVRL
                     LTSGIERTELERRKDRLADALYEAVPSGMGRGRTDLRMSKKELEEILVEGPAPLVERG
                     FGEAADLGRIESGGKLSGADPGAVSARAYERGLPQLGTLGSGNHFLEVQYVDEIYDEE
                     AAEAFGLREGWVTVLIHSGSRGLGHQVCQDYIERFLGAVPTYGIELVDRQLAAVPIES
                     PEGEEYLGAMASAANFAFANRQLISHFTREAFASAGFDHEGKRLRLLYDLAHNNAKFE
                     EHAGREVLVHRKGATRAFGPGEEDLPEEYRSVGQPVLVPGDMGRYSFVLAGTEGAMRD
                     TFGSSAHGAGRKMSRRKARKAAGSRDVVGEMKEKGILVRAAGKATVDEEISEAYKDAA
                     DVIEATHGAGIGRRVARLRPLIVVKG"
     gene            complement(2334..2747)
                     /locus_tag="VFT03_07535"
     CDS             complement(2334..2747)
                     /locus_tag="VFT03_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEU4848036.1"
                     /translation="MLDHTADVGFELRASTLEELFDEARRALLMVAFERPPQNGGEER
                     GIQLSAPDRETLLVRWLNELTYLIQDTGFVPAGARIRLEETGRAGSSLVASLTGAPVL
                     LEEYGWQGEIKSATYHGLDVSNDDEGWHARVILDV"
     gene            2897..>3185
                     /locus_tag="VFT03_07540"
     CDS             2897..>3185
                     /locus_tag="VFT03_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848037.1"
                     /translation="MGDHEAALQNEPVDVSEEFARQQNHFFIGSKVAALDPETASGMI
                     HWKGMALKQRVSYHQLTLEFEDYRVWWDTPPGEYEDEQEFPFSLSFVSPRTA"
BASE COUNT          521 a         1128 c          986 g          550 t
ORIGIN      
        1 cgccgacgac ctgctcgggg aggccgacac agcgctctac cgcgccaagc agtcaggccg
       61 gaaccgcgtc gcctcccacg aagatactcc tgcttgaggc tttcagccgc gtgtgccaga
      121 gcacggtctt cacgcgggcg acgagcccat cggaggagaa agtgggaaac tcacggttct
      181 cacacctccc cctgtctggc actctcgagc agctcgatga cctcctcgtc cgaggtctgc
      241 gagaagtcgc ggtaccagaa gcctattgcc ccgaggtcgg agggcgtctc gaggcagacg
      301 agctcgtcca cctcggggct gatcagcccg gccgtctgcg ccgcgcacac gggcgcggcg
      361 aggatcaaac gccgtggtcc aaggcgcctg agcgcctcga cggcggccct ggctgtgacg
      421 ccggtcgcga ggccgtcgtc gaccaggatc acggtccggt ccctgacctc gggctcggga
      481 cggtcgcccc tgaagtgcct gagccggcgc tcgacctccg ccagctcttt acgcgtgacc
      541 gcttccaggt agtcgtccgg gatgccgagc cgttcgacga cgttcccgtt gaggacgcgc
      601 acgccgccgg gggcgacggc ccctatgccg aactccggct ggcccggcgc cccgagcttg
      661 cgcgagacga agacgtccag cggcgcgcct agagagcgcg agatctcgta ccccacgggg
      721 acccctcccc gcgggagagc gaacacgacg gggcgctcgt ccctgtatcg ggtcagccgc
      781 tcggccagcc tgcgaccggc gtcctcccga tcctcgaacg gcggcccacc gtaaagcatc
      841 ctcaataacc tcctcaagtc ccggagcccc gcagcgctca gcccttgacg actatcagtg
      901 gcctgaggcg cgcgacccgg cgtccgatcc cagccccgtg tgtcgcctcg atgacgtcgg
      961 ctgcgtcctt gtaggcctcc gaaatctcct cgtcaaccgt ggcctttcct gcggcgcgca
     1021 ccaggatgcc cttctccttc atctccccga ccacgtcgcg gctcccggct gctttcctgg
     1081 cctttcggcg actcatcttg cggcccgcgc cgtgggcgct ggacccgaaa gtatcccgca
     1141 tcgcgccctc cgtgccggcg agcacgaagg agtagcggcc catgtcccct ggcaccagta
     1201 caggctggcc cacggaacgg tactcctcgg gaaggtcctc ctctccgggc ccgaaagccc
     1261 tggtggcccc cttgcggtgg accagtacct ccctgcccgc gtgctcctcg aacttggcgt
     1321 tgttgtgcgc caggtcgtag aggagccgca ggcgttttcc ttcgtgatcg aacccggcgg
     1381 aggcaaaggc ctccctggtg aagtgagaga tgagctggcg gtttgcgaag gcgaagttcg
     1441 cagccgacgc catcgccccg aggtactcct ccccctccgg gctctctatg gggactgcgg
     1501 cgagctgtct gtccaccagc tcgatgccgt acgtcgggac ggccccgagg aagcgctcga
     1561 tatagtcctg gcagacctgg tgcccgaggc cccgcgaacc ggagtggatc aggaccgtga
     1621 cccacccctc gcgcaggccg aacgcttcag cggcttcctc gtcgtagatc tcgtccacgt
     1681 actggacctc gaggaagtgg ttgccggagc ccagggtccc gagctgcggc aacccccgct
     1741 cgtacgccct ggcggagacc gcaccggggt ccgcgcccga aagcttcccg cccgactcga
     1801 tgcgtcccag gtccgccgcc tctccgaacc ctcgctcgac aagcggggca ggtccttcga
     1861 ccaggatctc ctcgagctcc tttttgctca tgcgaaggtc cgtgcggccc ctgcccatac
     1921 ccgaggggac ggcttcgtag agcgcgtcgg ccaggcggtc cttgcgccgc tccagctctg
     1981 tgcgttcgat acccgaggta agtagacgca cgccgcagtt gatatcaaaa ccgattccgc
     2041 cggggctgac cacgccgccc tcttgcggat cgaacgcggc taccccacct atcgggaagc
     2101 cgtagcccca gtggatatcg ggcatgctca gcgccggctc gactatgccg ggcagcgtcg
     2161 cgacgttctg gagctgcctc agggaggcgt agtcctcggc cgcgaggtcg ctcaatatct
     2221 tctccgaggc gtagaaagcc gcttcgaccc gcatcttgcc ctcgcggggg atgcggtacc
     2281 tgtactgacc aaggcgttcg aagttcatcg tcttcctcct acccctcaag aggtcagacg
     2341 tcgagtatca cccgcgcgtg ccagccttcg tcatcgttcg acacgtccaa cccgtgatac
     2401 gtcgcgctct ttatctcccc ctgccacccg tactcctcaa gcaggacggg agctccggtg
     2461 aggctcgcta ccaatgacga gccagcccgg ccggtctctt ctagtcgtat ccgtgcgccg
     2521 gcggggacga atcccgtgtc ctggatcaga tacgtcagct cgttcagcca gcgcacgagg
     2581 agcgtctcgc ggtcgggggc ggagagctgt atcccgcgtt cctctccgcc attctgcggg
     2641 ggacgctcga acgcgaccat gaggagggct cgccgggcct cgtcgaagag ctcttcgagc
     2701 gtggaggccc tcagctcgaa acccacgtcc gctgtgtgat caagcatctc tatcatctct
     2761 atcacggtgt cgcgattccc agacagaccg tatcagctta tcagcgccgc ccggaacata
     2821 gcttaacacc accggcgctt gaacccacag ccggaaactg ggattcttgg cgcgggagcg
     2881 agtagagggg agttcgatgg gagatcatga ggccgccttg cagaacgagc ctgtggacgt
     2941 gagcgaggag ttcgccaggc agcagaacca cttctttatc ggctcgaagg tcgcggccct
     3001 ggacccggag accgcctccg gcatgattca ctggaaaggc atggccctga agcagcgcgt
     3061 ctcctaccac cagctcacgc tcgagttcga agactacagg gtatggtggg atacgccgcc
     3121 gggggagtac gaggacgagc aggagttccc cttctcgctc tccttcgtga gcccgaggac
     3181 cgcgc
//
LOCUS       DASTUV010000375         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_504695, whole genome shotgun sequence.
ACCESSION   DASTUV010000375 DASTUV010000000
VERSION     DASTUV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504695"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VFT03_07545"
     CDS             complement(<1..998)
                     /locus_tag="VFT03_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEU4848038.1"
                     /translation="MQTERPVYEEIEELLGNVEPPAVALVEQRVDSPPALKDIRAAVG
                     EALRSVELPTGTVAVGVGSRGVGRISDVVSALVGFLKEAGAEPYVIPAMGSHGASTAE
                     GQADVLRHLGVSEERVGCPVRATMEAAKIGETPSGVSVYMDRNAFEADSVVVVNRIKP
                     HTAFRGTVESGPTKMLAIGLGKQRGADSIHAAGWGKIHETIPEAARVAVKSGKVAFGL
                     AVLENADEEPWRVAAIPADRLEEAEAPLLEEAKRKLMRLPFDEIDVLVIDEIGKNISG
                     DGADPNVTGRYPTPFASGGPKVERMVFLDLTPETGGNANGLGAADVVTERLVSRMDP"
     gene            1131..1868
                     /locus_tag="VFT03_07550"
     CDS             1131..1868
                     /locus_tag="VFT03_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU4848039.1"
                     /translation="MRVALFVPCYVDLINPEVGVSVVRVLRKLGVEVVYPEGQTCCGQ
                     PAFNSGFFNEARSLAGRFLDVFEDERWDYVVCPSGSCTTMVSHYYPFLMHDMPDERER
                     SEALGHRVREFSDFLVNVLGVKDLGARHAGKAVFHTGCHQRRELGVLEEPRELLRNVE
                     GLELLEWENEELCCGFGGTFSVKMPDVSTAMADEKVKALEKSGADALISCDSSCLLHL
                     GGRLERTGHDTRVLHLAQVLDSRGAGS"
     gene            1865..>2452
                     /locus_tag="VFT03_07555"
     CDS             1865..>2452
                     /locus_tag="VFT03_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU4848040.1"
                     /translation="MSEKLCFTPAVELASRIRRRDLSPVEAVEAFLARIEERNGTINA
                     YVTVIGDEAREAAREAERAVMAGDLLGPLHGVPVAIKDLFDYKAGVRNTFGSKPLAGH
                     VPSQNATYVERLEGAGAIVLGKTNTPEFGHKGATDNYIFGPTSTPFAPGKNAGGSSGG
                     SAAAVADGMCAIAQGTDAGGSIRIPASFCGAYGFKA"
BASE COUNT          381 a          830 c          818 g          423 t
ORIGIN      
        1 gggtccatcc tggacaccaa cctctcggtg accacgtcgg ctgccccgag gccgttggcg
       61 ttgcctccgg tctcgggcgt gaggtcgagg aagaccatgc gctctacttt cgggccgccg
      121 gaggcgaagg gggtagggta gcggccggtc acgttcgggt cggccccgtc gccgctgata
      181 ttcttcccga tctcgtctat cacgaggacg tctatctcgt cgaagggcag ccgcatgagc
      241 ttgcgcttgg cctcctccag aaggggagcc tcggcctcct ccagcctgtc cgcggggatg
      301 gcggcgaccc tccacggctc ctcgtcggcg ttctccagga cggcgaggcc gaaggcgacc
      361 ttgccgctct tcacggcgac ccgggcggcc tccgggatgg tctcgtggat cttgccccag
      421 cccgccgcgt gtatggagtc ggcgcccctc tgcttgccga ggccgatggc gagcatctta
      481 gtcggcccgc tctcgacggt accgcggaag gcggtgtgcg gcttaatccg gttgaccacc
      541 acgaccgagt cggcctcgaa ggcgttcctg tccatgtaga cggagacacc cgagggcgtc
      601 tccccaattt tcgcggcctc catcgtcgcc ctgacggggc acccgacgcg ctcctcgctc
      661 acccccaggt gcctcaagac gtccgcctgg ccctcggccg tcgaggcccc gtggctcccc
      721 atagcaggga ttacgtaggg ctcggctccg gcctctttga ggaaccctac gagcgccgag
      781 accacgtcgg atatcctgcc taccccgcgg ctcccgaccc ccacggcgac cgttccggta
      841 ggtagctcga cggagcgtag cgcctccccg accgccgccc ggatgtcctt cagcgccggc
      901 gggctgtcaa cgcgctgctc gacgagcgcc accgcgggcg gctcgacgtt gccgagcaac
      961 tcctcgatct cctcgtagac cggcctctcc gtctgcatgc catcaccccc ggatagagat
     1021 ccttgacatc tattctatca accggaacgc ggccagagtc ctcggtccct tccggttgct
     1081 atactggcta gtgttggtag ggggtagcaa gaaggaaggg gcttaaagcg gtgagggtag
     1141 ctctcttcgt gccgtgttac gttgacctta taaacccgga ggtcggcgtg agcgtggtgc
     1201 gtgtactgcg caaacttggc gtcgaggtcg tctaccccga ggggcagacc tgctgcggcc
     1261 agccggcctt caactccggt ttcttcaacg aggcccgcag cctcgccggc cgcttcctcg
     1321 acgtcttcga ggatgagcgc tgggactacg tcgtgtgccc ttccggctcg tgcaccacca
     1381 tggtctccca ctactacccg ttcttgatgc atgacatgcc ggacgaacgg gaacgctccg
     1441 aggctctcgg acatcgcgtc cgggagttct cggatttcct cgtgaacgtg ctgggggtga
     1501 aggacctcgg ggcccggcat gcggggaagg cggtcttcca cacagggtgt caccagcggc
     1561 gggaactcgg cgtgctcgag gagccgcggg agttgctgcg gaacgtcgag ggactggagc
     1621 tcttagagtg ggagaacgag gagctctgct gcgggttcgg ggggacgttc tccgtcaaga
     1681 tgcccgacgt ctcgaccgcg atggctgacg agaaggtaaa ggccctggag aagagcgggg
     1741 ctgacgccct gatctcctgc gactccagct gccttttgca ccttggcgga cgcctcgagc
     1801 gcacgggcca cgacacccgc gtcctgcacc tggcgcaggt actggattcg aggggggcgg
     1861 gatcgtgagc gagaagctct gtttcacccc ggcggtcgag ctggcgtcga ggatccggcg
     1921 ccgcgacctc tcgccggtcg aggccgtcga ggccttcctc gcacggatcg aggagcgcaa
     1981 cggcacgata aacgcttacg tcaccgtcat cggcgacgag gcgcgcgagg cggccagaga
     2041 ggccgagcgc gccgtcatgg ccggagacct gctcggcccg ctgcacgggg tcccagtggc
     2101 gataaaggac ctcttcgact acaaggcggg ggtgaggaac accttcggct cgaagcctct
     2161 tgccggccac gtgccgagcc agaacgcgac ctacgtcgag cgcctggagg gagcgggcgc
     2221 gatcgtgctc ggcaagacca atacgcccga gttcggccac aagggggcga cggacaacta
     2281 catcttcggc ccgacgagca cgccgttcgc ccccggcaag aacgcgggcg gctcctcggg
     2341 cgggagcgcc gccgccgtcg cggacggcat gtgcgccata gcccagggca cagacgcggg
     2401 aggctctatc cgcatccccg cctccttctg cggcgcctac ggtttcaagg cc
//
LOCUS       DASTUV010000376         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1577230, whole genome shotgun sequence.
ACCESSION   DASTUV010000376 DASTUV010000000
VERSION     DASTUV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577230"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..173
                     /gene="rpmG"
                     /locus_tag="VFT03_07560"
     CDS             24..173
                     /gene="rpmG"
                     /locus_tag="VFT03_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4848041.1"
                     /translation="MRQLVTLACEECKRRNYHTRKNRRNTPDRIQLKKYCPWDRKHTI
                     HRETR"
     gene            187..259
                     /locus_tag="VFT03_07565"
     tRNA            187..259
                     /locus_tag="VFT03_07565"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:Trp,seq:cca)
     gene            292..546
                     /gene="secE"
                     /locus_tag="VFT03_07570"
     CDS             292..546
                     /gene="secE"
                     /locus_tag="VFT03_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU4848042.1"
                     /translation="MGKKRGSATKGGAKKGQQQTKQGFIAGPRKFAREVQGELKRVTW
                     PDRDQLRQSTAVVLIIVLTLTAYVYVWDTLFRSLARLIFA"
     gene            565..1098
                     /gene="nusG"
                     /locus_tag="VFT03_07575"
     CDS             565..1098
                     /gene="nusG"
                     /locus_tag="VFT03_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU4848043.1"
                     /translation="MKKWYVVNTYSGHENKVRTTMERRIESLGLGRYFGEFSIPTESV
                     IEIKNGKKVPTTQRQFPGYILINMDMNENTWQLVRQTPGVTQIVGSGDRPTPLSRAEV
                     ERLLHPGEAAEQREKVKTTVDYQIGETVKVIGGPLSDFTGSISDINVDQSRLKVLVSI
                     FGRETPVELSFNQVAKL"
     gene            1114..1551
                     /gene="rplK"
                     /locus_tag="VFT03_07580"
     CDS             1114..1551
                     /gene="rplK"
                     /locus_tag="VFT03_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU4848044.1"
                     /translation="MGRGRGRRVARKLKLQITGGQANPAPPVGPALGQAQVNIGEFVR
                     QFNEATRDQMGQVVPVEITVYEDRSFTFITKTPPAAFLLKQAAGLDKGSGEPNREKVG
                     TVTRAQVEEIAKTKMPDLNAADIDGAARIVEGTARSMGITVNG"
     gene            1556..2233
                     /gene="rplA"
                     /locus_tag="VFT03_07585"
     CDS             1556..2233
                     /gene="rplA"
                     /locus_tag="VFT03_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU4848045.1"
                     /translation="MLEQKTRIDPTRLYHPREALELLKEFDGAKFDESVEAHIHLNVD
                     PRRADQMVRGTLMLPNGTGKTRRVAVFAVGDKAREAEEAGADVVGSEDLAERVREQGF
                     MDFDVAVATPDQMRIVGPLGQILGPRGLMPNPKSGTVTQDVGRAVEEIKRGKIEYRVD
                     RYGIIHGIIGKKSFDLDSLVGNYFALREELQRARPAPVKGRYFKSVALTTTMGPSIKV
                     DPAVEKD"
     misc_feature    2285..2416
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2425..2946
                     /gene="rplJ"
                     /locus_tag="VFT03_07590"
     CDS             2425..2946
                     /gene="rplJ"
                     /locus_tag="VFT03_07590"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU4848046.1"
                     /translation="MKREEKARIIQEIAEKLRGNSAVLVDYQGMNVAQSTQLRARSRE
                     AGVEFVVAKNTLTRMAADEAGVEDLSQYLVGPTALAFSEDPVASAKLMAEFAEQVESF
                     ALKGGLLEGSRVLNEGEVSALSRLPGREQLLAQVVGGISSPLTGLVTVLNNTVQGLVV
                     ALNQIAEQKGAQS"
BASE COUNT          650 a          871 c         1039 g          464 t
ORIGIN      
        1 cccctgtgat tcggaggtag attgtgcgac aattagtaac gctggcatgt gaggagtgca
       61 agcgccggaa ctaccacacc cgtaagaacc ggcgcaacac cccggaccgg atccagctaa
      121 agaagtattg cccgtgggat aggaagcaca ccatccaccg ggagacccgc taggggtctt
      181 aagcggaggg cagtagctca gttggtagag cagcggtctc caaaaccgca ggtcgggggt
      241 tcgagtccct cctgccctgc tcttgtcgtt caaagtcagg tgagtagata gatgggcaag
      301 aagcgtggct cggccaccaa gggcggggcc aaaaaaggac aacagcagac gaagcagggc
      361 ttcatagccg gtccgaggaa gttcgcccgg gaggtccagg gtgaactcaa gcgggtcacc
      421 tggcctgatc gagaccagct caggcagagc acggcggtgg tcctgatcat cgtccttacg
      481 ctgacagcct acgtgtatgt ctgggacacg ttgttccgga gtctggcgcg tttgatcttc
      541 gcgtagtaac caggagcgtt agttttgaag aagtggtacg tagtcaacac atactccggg
      601 cacgagaaca aggtccggac gacgatggag cgccggatcg agtcgctcgg cctgggcagg
      661 tacttcgggg agtttagcat ccccacggag agcgtcatcg agatcaagaa cggcaagaag
      721 gtcccgacga cgcagcgcca gttcccgggc tacatcctga tcaacatgga catgaacgag
      781 aacacctggc agctcgtgcg ccagacgccg ggcgtcaccc agatcgtcgg ttcgggggac
      841 aggccgacgc ctctaagtag ggcggaagtc gagcggctcc tgcatccggg cgaggctgcg
      901 gagcagcgcg agaaggtcaa gacaacggtg gactaccaga tcggggagac ggtcaaggtc
      961 atcggcgggc cgctctcgga cttcaccggc tcgatatccg acatcaacgt ggaccagtcg
     1021 aggttgaagg tcctcgtctc catcttcggg cgcgagacgc cggtggagct ctcgttcaac
     1081 caggtagcca agctctagaa ggagaagcgt tttatgggta gagggcgcgg acggcgcgtc
     1141 gcgcgcaagc tgaagttgca gatcaccggg gggcaggcga acccggcccc gccggtcggc
     1201 cccgcgttgg gccaggcgca ggtgaacatc ggggagttcg tccggcagtt caacgaggcc
     1261 acgcgcgacc agatgggcca ggtggtcccg gtggagatca cggtctacga ggaccgctcg
     1321 ttcaccttta taaccaagac gccccccgcg gcgttcctgc tcaagcaggc cgcggggctc
     1381 gacaagggca gcggcgagcc gaaccgcgag aaggtcggca ccgtgacccg cgcgcaggtc
     1441 gaggagatag cgaagaccaa gatgccggac ctgaacgccg ccgacatcga cggcgcggcg
     1501 aggatagtcg agggtacggc ccggagcatg gggataacgg tcaatgggta agcggttgct
     1561 ggagcaaaag acgaggatag accccacgcg gctctaccac ccgcgcgagg cgctcgagct
     1621 cctcaaggag ttcgacggcg cgaagttcga cgagagcgtc gaggcgcaca tccacctgaa
     1681 cgtggaccca aggcgggcgg accagatggt gcgcggaacg ttgatgctgc cgaacggcac
     1741 gggcaagacg cgcagggtcg ccgtcttcgc cgtcggcgac aaggcgcgcg aggccgagga
     1801 ggccggggct gacgtcgtcg gctccgagga cctcgccgag cgcgtccgcg aacagggctt
     1861 catggacttc gacgtggcgg tcgctacccc cgaccagatg aggatcgtcg gtccgctggg
     1921 ccagatcctt ggtccccgcg ggctcatgcc gaaccccaag agcggcacgg tcacgcagga
     1981 cgtgggccga gcggtcgagg agatcaaacg cggcaagatc gagtaccgcg tggaccgcta
     2041 cggcatcatc catggcatca tcggcaagaa gtccttcgat ctggacagcc tggtcgggaa
     2101 ctacttcgcc ctgcgcgagg agctccagag ggcgcgcccc gcgccggtga agggacggta
     2161 cttcaagtcc gtggccctca ccacgacgat gggaccctcg atcaaggttg accccgcggt
     2221 ggagaaggat taacatcacg cgcggactct agatgactct agatgactct agatgactct
     2281 agatgactct agatatatag gaccacagac agccggggcc cgtgcgaggg cttaatcatc
     2341 cggccgaggt cgcaaggttt tacgggagtt agaagccccg ggcgcctctg cggccccggg
     2401 gtttttagtt agcggaggag aagtgtgaaa cgggaagaga aggcgcggat aatacaggag
     2461 atagccgaga agctgcgcgg caactcggca gtgctcgtcg actaccaggg gatgaacgtg
     2521 gcccagagca cccagcttcg ggcgcgcagc cgcgaggccg gcgtcgagtt cgtcgtcgct
     2581 aagaacacgc tgacccgcat ggcggcggac gaggccgggg tggaggacct ctcgcagtac
     2641 ctggtcgggc cgacggcact cgccttctcc gaggaccccg tcgccagcgc gaagctgatg
     2701 gccgagttcg ccgagcaggt cgagtctttc gcactgaagg gtggactcct cgagggcagc
     2761 cgggtgctga acgagggcga agtctcagcc ctcagcagac tgccggggcg agagcagctg
     2821 ctcgcacagg tcgtcggagg catctcctcc ccgctcacgg ggctggtcac ggtgctcaac
     2881 aataccgtcc agggtctggt agtggcgctg aaccagatcg ccgaacaaaa aggcgcgcag
     2941 tcgtaggcaa caagttcagg aggacttgag atggcgataa atcaggaaga gttgatggag
     3001 actatcgaga acatgacggt tctc
//
LOCUS       DASTUV010000377         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_389960, whole genome shotgun sequence.
ACCESSION   DASTUV010000377 DASTUV010000000
VERSION     DASTUV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389960"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFT03_07595"
     CDS             complement(<1..553)
                     /locus_tag="VFT03_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4848047.1"
                     /translation="MNERIHAGRVALITGGGRGIGAATARLLAKRGAAVALAARTEEE
                     VVSVAAQIEALGATALPLVVDVAEEDSVASGFERAQAELGPITILINNAGTPGVPLPV
                     AAMEAAQWRRVFEVNVTGAFLCAREALPQMAAENWGRIVNVSSAAARHPLAGMAAYGA
                     SKAALDQLTRVLALEGAPYNIVTT"
     gene            630..1283
                     /locus_tag="VFT03_07600"
     CDS             630..1283
                     /locus_tag="VFT03_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU4848048.1"
                     /translation="MSDIPLFPLNVVLMPGAPLPLHIFEDRYKQMVNECLEQESEFGM
                     VLADESGTRRVGCTARIVELVERYEDGRMLILVEGSRRFKLNSILTGKPYYVGEVEYL
                     EEEPEEDVPALAEECIALLERVVEAATEGSVGIEIQPPYRNLSFAIAGRIDFEVETRQ
                     QILELTSERGRLEKVKELLSAAAERLEREQQAREKAQKNGHLRGDWRPSEEKEEPDG"
     gene            complement(1312..2346)
                     /locus_tag="VFT03_07605"
     CDS             complement(1312..2346)
                     /locus_tag="VFT03_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU4848049.1"
                     /translation="MFLTLLQNTSKETTAGLKDAAGDAVSDTAHDAGREISRVQQFFG
                     EVYGYFFSTDFLANVIASALVVLFGLLAYRVVTHGLPRMLRWRRRRAEDLLDAESVAR
                     IKRQDTAITLIRNALRYVIFIIVALFILSIFVRNPLPATAGATLIAAIIGFGAQSFLR
                     DVIAGFSIIFEGQYSVGDFIEVQPQGVSGIVEELGLRMTKIRSLSGEVSFVPNGVVQG
                     VTNYVSGQQRFTIEVQLSDAEAVPRVLESLDEASELYIAPPRLVGREETGGRTRLRIV
                     AGVLPSMAWLIEEHLVERIKAAAGEDALAAPPLVYKVDQANLRRIRELLPKEVEKNLP
                     PEAEKKLRGS"
     gene            2472..>2732
                     /locus_tag="VFT03_07610"
     CDS             2472..>2732
                     /locus_tag="VFT03_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848050.1"
                     /translation="MKRAGLLIIAVAVALLLVVSTIAVARTFTCDQNRCRGTNDRDTI
                     FGTRVDNTIHSLEGADLVRGNGGRDSLHGDGGKDRLSGGSGDD"
BASE COUNT          495 a          854 c          947 g          436 t
ORIGIN      
        1 ctgtggtgac tatgttgtag ggcgctcctt cgagggcgag gacgcgggtg agctggtcga
       61 gggcagcctt cgaggcgccg taggcggcca taccggccag cgggtgcctc gcggcggcgc
      121 tcgagacgtt cacgatacgg ccccagttct cggcggccat ctgcggcagc gcctccctcg
      181 cgcagagaaa ggcccccgtg acgttgacct cgaagacacg gcgccactgc gccgcctcca
      241 tggcggcgac cgggagcggg acgccggggg tgcccgcatt gttgatcagg atggtgatgg
      301 ggccgagctc ggcctgcgcc cgctcgaagc cggaggcgac cgagtcctcc tctgcgacgt
      361 cgacgacgag cgggagggcc gtagcgccca gggcttcgat ctgtgccgca acggagacga
      421 cctcctcctc ggtgcgcgcg gcgagagcga cggcggcgcc gcgtttggcg aggagcctcg
      481 ccgtcgccgc tccgataccc cgtccgccgc ccgttatcag ggccacgcgg cccgcgtgta
      541 tcctctcgtt cacgctacca tcatacccgg cgcggagatc cggcgaatcg taagataaaa
      601 tgtaggcaaa tagcggaagg agcatgcgcg tgtccgacat acctcttttc ccgctgaacg
      661 tcgtgctgat gccaggggcg ccgctgcccc tgcacatatt cgaggaccgc tacaagcaga
      721 tggtcaacga atgtctggag caggagagcg agtttgggat ggtgctggcc gacgagtcag
      781 ggactcggcg cgtgggctgc acggcccgga tcgtcgagct cgtagagcgc tacgaggacg
      841 ggcggatgct gatcctggtc gagggctcgc ggcgcttcaa gctcaacagc atcctcacgg
      901 gcaagcccta ctacgtgggg gaggtcgagt acctcgaaga agagccggag gaggacgtgc
      961 cggcgctcgc cgaggagtgc atagcgctgc tcgagcgggt cgtggaggcg gcgacggagg
     1021 gctcggtcgg catagagata cagccgccat accggaacct ctcgttcgcg atcgccgggc
     1081 gcatagactt cgaagtagag acccggcagc agatcctgga gctcacctcc gagaggggac
     1141 ggctggagaa ggtcaaggag ctcctctcgg ctgcagccga gaggctcgag cgcgagcagc
     1201 aggcgcgcga gaaggcccag aagaacgggc accttcgcgg cgactggcgg ccgagcgagg
     1261 agaaggagga gccggacggc tagtgcagag gggcggttcg cggaccgccc ttcaggagcc
     1321 tcggagcttc ttctctgcct ctggaggcag gttcttctcg acctctttcg gcaggagctc
     1381 cctgatcctg cgcaggttgg cctgatcaac cttgtagacc agagggggag ctgcgagagc
     1441 gtcctcgccc gccgcggcct ttatgcgctc gaccaggtgc tcctcgatga gccacgccat
     1501 cgagggcagg accccggcca cgatgcgcag ccgtgtcctg ccgccggtct cctcgcgtcc
     1561 caccagtcgc ggcggggcga tgtagagctc tgaggcctcg tcgagggatt cgaggaccct
     1621 gggaaccgcc tcggcgtccg agagctggac ctcgatcgtg aagcgctgct ggcccgagac
     1681 gtagttggtg acaccctgca cgacgccgtt gggtacgaag ctcacctccc ccgagagcga
     1741 ccgtatcttg gtcatcctga gccccagctc ctccacgatg ccggagacgc cttgcggttg
     1801 cacctcgata aagtccccga cgctgtactg gccctcgaag atgatcgaga acccggctat
     1861 aacgtcgcgc aggaagctct gcgcgccgaa acctatgatc gcggcgatca gcgtcgcgcc
     1921 agccgttgca ggcagcgggt tgcggacgaa gatgctcagg atgaacagag ccacgatgat
     1981 aaagatcacg taccgaaggg cgttccggat cagggtgata gcggtgtcct ggcgctttat
     2041 gcgggccacc gactccgcgt cgagcagatc ctccgccctg cgccgccgcc acctgagcat
     2101 gcggggcagc ccgtgtgtca ccacccggta ggccaagagc ccgaagagga ccacgagcgc
     2161 ggaggcgatc acgttggcca ggaaatccgt gctgaagaag tagccgtaga cctccccgaa
     2221 gaactgctgt actcgtgaga tctcccgccc cgcgtcgtgg gctgtgtcgc ttacggcgtc
     2281 cccggcggcg tctttcaacc cggccgttgt ctctttggac gtgttctgaa ggagcgtcaa
     2341 gaacatggcg gcgcacatta aagtacccgg atgctccgac cgcaaggggt gtatgacccg
     2401 cgatcgggcc gctatactgc tcgcggtacc ggtcgagacg ctccggctca cggagagaag
     2461 gaggatggag ggtgaagaga gcgggccttt tgattatcgc tgtggcggta gcgttgctac
     2521 tcgtcgtcag cactatagcc gtcgcgcgca cgtttacttg cgaccagaac aggtgccgcg
     2581 gcacgaacga cagagacacg atattcggca cccgcgtgga caacaccatc cactcgttgg
     2641 agggggcgga tctggtacgc ggcaacggcg gaagggactc cctgcacggc gacggcggca
     2701 aggaccggct gagcggcgga agcggcgacg ac
//
LOCUS       DASTUV010000378         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_257342, whole genome shotgun sequence.
ACCESSION   DASTUV010000378 DASTUV010000000
VERSION     DASTUV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257342"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFT03_07615"
     CDS             <1..928
                     /locus_tag="VFT03_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEU4848051.1"
                     /translation="ADTAKALVVIYLAISAVETVCLLAAGMPLYDSVVHTFGTVATGG
                     FSNKAASIGFYDSVAIEAIIIFFMTASGVSFSLYYLLYTRRRLDVVLDRELLAYLGII
                     AAAALLVWGILVFEGDYGDNIGQALRESAFTVSSIITTTGFVTADFDRWDPAAKVVIV
                     LLMFVGGCAGSTAGGIKVIRVLIVARTILQDIFRMIHPRAVTPMKLGGRVVPESVRIA
                     ALGLFAAWIGVFGLATLLVALQDNLTLTSSATAVATTLNDVGPGLDQVGATESFEIVD
                     YFGRIVLTVCMLLGRLEIFTVLALLSPAFWRR"
     gene            complement(950..1867)
                     /gene="rocF"
                     /locus_tag="VFT03_07620"
     CDS             complement(950..1867)
                     /gene="rocF"
                     /locus_tag="VFT03_07620"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEU4848052.1"
                     /translation="MFEGARRPPGIFGVPIDLGQDRRGVDMGPSAIRYARLQGALEEL
                     GYQVSDLGNAVTPLPETVEKEGDIRHLEAVRDVCEEVAEQAEAMISSGLFPIFLGGDH
                     SIAIGTVSGVARNFERIGVIWLDAHADFNTPQTSPSGNIHGMPLSVLTGAGHPDLVAI
                     GGEGASVRTEDVVIVGLRSVDLEERRLLNDAGIRVYTMKEVDAYGIASVVRRALRALS
                     HLDRVHVSLDLDVVDPDVAPGVGTPVRGGLTYREAHLVMELINEAGIAASLDVVEINP
                     ILDQRNGTAELAVELVASLMGRQIIGIPT"
     gene            complement(1860..>2805)
                     /locus_tag="VFT03_07625"
     CDS             complement(1860..>2805)
                     /locus_tag="VFT03_07625"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU4848053.1"
                     /translation="EAIAEGGAELMYEGELARRLSGYILKMGGIITERDLAEYQAVIR
                     KPLSVAYGHGDIYTNGPPSAGGPTLAQMLRIVSGYDLVAMSSDDYARVLAGAMRFALR
                     DREIAYVDGAENEIVAQRLTSEEYAKQQRRRIFGSPQTTHISCVDAEGAAVSITATMG
                     YGSGLVVPGTGIPMNNTLGEPELNPRGFHALEPGERLISSMSPTIVSSQEGGLISLGS
                     AGASRIPTAILQTLVNVLDFGTPLETAVFAPRFHAEGDLLAYEAGAQTTDLDRYEQVL
                     TFEGPNMYFGGVNAVRRTPEGTFEAAADPRRSGGVAFV"
BASE COUNT          446 a          925 c          907 g          527 t
ORIGIN      
        1 cgcagatacc gccaaggccc tcgtcgtcat ctacctcgcc atctccgcag tcgagaccgt
       61 ctgcctgctc gcggcgggga tgcccctcta cgactccgtg gtccacacct tcggtacggt
      121 ggccacggga ggattcagca acaaggcagc gtctataggc ttctacgatt cggtggccat
      181 cgaggcgatc atcatcttct tcatgacggc ctccggcgtg tccttctcgc tctactacct
      241 gctctacacc cggcgccggc tcgacgtggt tctcgaccgt gagctactgg cttacctcgg
      301 gatcatcgcg gcggccgcgt tgcttgtgtg gggcattctc gtcttcgaag gggactacgg
      361 ggacaacatc ggccaggcac tgagggagtc ggcgttcacg gtctcgagca tcatcacgac
      421 gacggggttc gttacggcag acttcgaccg gtgggacccc gcggcgaagg tggtgatcgt
      481 attgctgatg ttcgtcgggg ggtgcgcagg ctccaccgcg ggaggcatca aggtcatcag
      541 ggtgctcatc gtcgcccgga cgatcttgca ggacatcttc aggatgattc acccgcgcgc
      601 cgtcacaccc atgaagctgg gcgggcgcgt ggtacccgag agcgtccgga tagccgctct
      661 cgggctcttt gcagcctgga tcggcgtgtt cggccttgcg acactcctgg tggcgctcca
      721 ggataacctg acgctcacct cctcggcgac cgcggtcgcg acgacgctca acgacgtcgg
      781 accggggctg gatcaggtcg gggccacgga gagtttcgag atcgtcgact acttcggccg
      841 catagttctc acggtctgca tgctcctcgg gaggctggag atcttcacgg tgctcgcgct
      901 gctctctccg gcgttctggc ggcgttgaag ggcgattcgc gagacgcccc tacgtgggga
      961 tgcctatgat ctggcgcccc atcaggctcg ccacgagctc gaccgcgagc tcggcggtcc
     1021 cgttcctctg gtcgaggatg gggttgatct ccacgacgtc gagcgaggct gcgatccccg
     1081 cctcgttgat gagttccata accaggtgcg cttcccggta ggtcagaccg ccgcgcaccg
     1141 gcgtcccgac gccaggggcg acgtccggat ctaccacgtc gaggtctagc gagacgtgga
     1201 cccggtcgag gtgcgagagg gccctgaggg cgcggcgcac gacgctcgcg atcccgtagg
     1261 cgtctacctc cttcatcgtg tagacgcgga tgccggcgtc gttgagcagg cgcctttcct
     1321 ccagatccac ggaccgaagc cccactatca cgacgtcttc tgtgcgcacg ctggcgccct
     1381 cgccaccgat agccacgagg tcgggatggc cggctcccgt gagcacggag agcggcatgc
     1441 cgtgaatgtt ccccgagggc gaggtctgcg gggtgttgaa gtcggcgtgg gcgtcgagcc
     1501 agatgacccc gatgcgctcg aagttcctgg cgacccccga gacggtcccg atcgcgatcg
     1561 agtggtcgcc accgaggaag atcgggaaga gcccactcga gatcatggct tcggcctgtt
     1621 cggcgacctc ctcgcagacg tcccgaacgg cttccaggtg ccgtatgtcc ccttctttct
     1681 ccaccgtctc gggtagcggg gtcacggcgt tgccgagatc ggacacctgg tagccgagct
     1741 cttcgagggc gccctgcagc cgggcgtagc ggatagccga gggtcccatg tccacgccgc
     1801 ggcggtcctg gccgagatct atcggtacgc cgaatatacc cggcggcctg cgcgcgccct
     1861 caaacaaagg ccacaccccc cgagcgccgc ggatcggctg cggcctcgaa ggttccttca
     1921 ggggtgcgcc gtacggcgtt gaccccgccg aagtacatgt tcggtccctc gaaggtgagg
     1981 acctgctcgt agcggtcgag gtcggtcgtc tgcgcgcctg cctcgtaggc taagaggtcg
     2041 ccctcggcgt ggaagcgagg ggcgaatacg gctgtctcca gcggcgtccc gaagtcgagc
     2101 acgttcacga gggtctgcag gatagccgtc ggtatccgtg aagcgccggc agaaccgagc
     2161 gagatcaaac ctccctcctg cgatgagaca atagtcgggc tcatgctcga gatgaggcgc
     2221 tcgccaggct ctagcgcatg gaagcctctg gggttgagct ccggctcgcc tagagtgttg
     2281 ttcatcggaa ttcctgtacc ggggacgacg agccctgacc catagcccat agtggcggtt
     2341 atggagacgg ccgccccctc ggcgtccaca cacgagatgt gcgttgtctg cggcgagccg
     2401 aagattcgcc ggcgctgttg cttcgcgtac tcctcgctcg ttaacctctg ggccacgatc
     2461 tcgttctccg cgccgtccac gtacgcgatc tcccgatcac gcagagcgaa cctcatggct
     2521 ccggccaaca ccctcgcgta gtcgtccgag gacatggcga ccagatcgta acccgagacg
     2581 atcctgagca tctgggcgag cgtcggtccc cccgcggagg gcggtccgtt ggtgtagatg
     2641 tcaccatgcc cgtaagctac ggagaggggc ttgcgaatga cagcctggta ctcggcgagg
     2701 tccctctccg tgatgatccc acccatcttg aggatgtagc ctgagaggcg ccgcgcgagc
     2761 tcgccctcgt acatcagctc cgcgcccccc tccgctatcg cctcc
//
LOCUS       DASTUV010000379         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1504297, whole genome shotgun sequence.
ACCESSION   DASTUV010000379 DASTUV010000000
VERSION     DASTUV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504297"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1230
                     /locus_tag="VFT03_07630"
     CDS             379..1230
                     /locus_tag="VFT03_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4848054.1"
                     /translation="MGKSLVYTSLLLVVTAIWGWTFVVVQDAILGFGVLGFLALRFTL
                     ASLALSPILLSGITRRTLLVGGGIGLVLAMGYLFQTLGLLYTTPTNSGLITGLFVVFA
                     PLADRIFFGRRASRQVVVAVALSLVGMALLAGGGPEGVNPGDLLTLLCAAALGLHIAL
                     LSRYAAGHDAAGLTLAQIVAMAILFAALWPVAGTVELPPPEVWVALLITGLLASAGAF
                     YVQTFVQQRLSAARTAVIMTMEPVFAALFGYWLAGDRLVAVQVAGAGLILSALFVGEV
                     LPLLRRK"
     gene            1592..2257
                     /locus_tag="VFT03_07635"
     CDS             1592..2257
                     /locus_tag="VFT03_07635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU4848055.1"
                     /translation="MERTIDVPEYEVQDGVRVDPSRTALIIGDMQNDFVKEGGTLVVP
                     DAEGTIPAIAGLLDQARETGMRVVFVQDTHRDGDPEWEIWGKHCREGSWGWQVVDELA
                     PREDEVSVRKPRYDAFYGTSLEHSLRIWGIDTLVICGTVANICVHYTASSAAMRWFNV
                     VVPKDCISSFNAFDMEASLSQTATLLGEVVASGGDVEIGKPETAEVYLSKLEEAATRE
                     RGR"
BASE COUNT          397 a          712 c          741 g          463 t
ORIGIN      
        1 tcctccttct ccctaacctt gacttgttcc acggaacact caggatacac cacgtgagac
       61 taccgtgtat aagagccgtt gctggtagcc cgtagggtag ccccgcacct ccacatcctc
      121 tggtgtggct acgccctacg cttcgacgta gggactcaag cccttcgcac gcatgctctc
      181 tgtagccaga gtacgtccag ccgtgttgag gtacgcagtt catgccgctc agttccgtga
      241 atgaagaagc caaactgctc gaccatgccg aaggagccac aggtaagcta cttccagggg
      301 caattttgct gactgcttat ttcgggcttc ttgtatggta ttctcctcgg actgtggaag
      361 ccgaaaacgg gcattctatt gggcaaaagt ctcgtctaca catctctgct attagtcgtc
      421 acggcgatct ggggctggac gttcgtcgtg gtgcaggatg cgatcctggg cttcggcgtc
      481 ctcggattcc tcgcgctgcg cttcactctc gcgagcctcg ctctctcccc aatactgctc
      541 tcaggcatta cgcgccggac cttgctcgtc ggtggaggca tcgggctggt cctcgcgatg
      601 gggtacctct tccagacgct cgggctcctc tacaccacgc cgaccaacag cgggctcatc
      661 acgggcctct tcgtcgtctt cgcgcccctt gcggacagga tcttcttcgg cagacgggcc
      721 tcccggcagg ttgtcgtcgc ggtcgctttg agcctggtcg ggatggcgct cctggccggg
      781 ggcgggccgg agggcgtgaa ccccggtgac ctgctgaccc tcctgtgcgc ggccgcgctc
      841 gggctgcaca tagcgctgct ctcccgctac gcggccggac acgacgctgc cgggctcacc
      901 ttggcgcaga tagtcgcgat ggcgatcctc ttcgccgccc tctggcccgt ggccgggacg
      961 gtggagttgc cccctcccga ggtgtgggtg gcgctgctta tcacggggct tctcgcatcc
     1021 gcgggagcgt tctacgtcca gacgttcgtc cagcagcggc tgtccgccgc ccggacagcc
     1081 gtcatcatga cgatggaacc cgtcttcgcc gctcttttcg gctactggct cgccggagac
     1141 aggctcgtcg cggtgcaggt cgccggggcc gggctcatcc tatccgcgct gttcgtcggg
     1201 gaggtcctgc ccttgctccg gcgcaagtag cggagaaaac gaggtcatgg acccgattcg
     1261 atccggtcga ggcgtgcctc gataggagat tttgactatg cttggtcccg caggggagag
     1321 gcgcctcact cggagggtga acctcgagca ttcggggcaa ctgggagaag catcgcgagg
     1381 tgctcgcccg ccgaagaccg tctgccgccg ggtgccatca cgaacgagtg gaggtctacg
     1441 tctgcgtaga cctccaacct ggcaacctct cttcaagagg gagcgtagct tttttgcctt
     1501 aatggtggcg aaatgttagc agtgctcggg acgcgttata aactgatggt ggaatcagga
     1561 gctctcgtcg aagcgtagaa ggaggccgct catggagcgc accattgacg tcccggagta
     1621 cgaggttcag gacggcgtcc gggttgaccc gagccggacc gccttgatca tcggggacat
     1681 gcagaatgac ttcgtgaagg agggcggcac gctcgtcgta cctgacgccg agggaaccat
     1741 cccggcgata gcgggcttgc tggaccaggc gcgggagacc gggatgaggg tggtcttcgt
     1801 ccaggatacg cacagggatg gtgaccctga gtgggagatc tggggcaagc actgccgcga
     1861 ggggagctgg ggttggcagg tggtagacga gctcgccccg cgcgaagacg aagtaagtgt
     1921 ccgcaagccc cgttacgatg ccttctacgg cacgtcgctc gagcactcac tgcgtatttg
     1981 gggcatcgat acgctcgtca tctgcggaac ggtcgccaac atctgcgtcc actacaccgc
     2041 ctcgagcgcc gccatgaggt ggttcaacgt cgtagtgcca aaagactgca tctcgagctt
     2101 caacgccttc gacatggagg cgtccctgag ccagacggcc accttgctcg gagaggtcgt
     2161 cgcgagcggc ggagatgtcg agatcgggaa gccggagacc gcggaagtct acctgagcaa
     2221 gctcgaagag gccgctactc gcgaacgggg ccggtaagaa ctcacatcgc tgacacgcgc
     2281 gggctctggt agcattccgc gctcgaccgg tag
//
LOCUS       DASTUV010000380         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_214658, whole genome shotgun sequence.
ACCESSION   DASTUV010000380 DASTUV010000000
VERSION     DASTUV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214658"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..621
                     /locus_tag="VFT03_07640"
     CDS             67..621
                     /locus_tag="VFT03_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4848056.1"
                     /translation="MDESPTTPGEGPALPGLRGVDHIGITVPNMEQATAFFVDVLGCE
                     LLYEREPPGDDAPRHRLGVPAGSRIQGVRFLRCANGANIELFEFYSPDQREEFPRPSD
                     VGIQHLAVYVEDLDAAVEHLRSHEVNLMSGPNPLPGPEAGEGNRFIYARTPWGLTLEL
                     ISYPAPMAYQQRSDARRWRPPAGQ"
     gene            complement(787..1575)
                     /locus_tag="VFT03_07645"
     CDS             complement(787..1575)
                     /locus_tag="VFT03_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU4848057.1"
                     /translation="MATNPIREIWAGDRPAFGLWSVMPGTTGAEILAKAGADYVCVDQ
                     QHGVIDYGSMAPMFQAIRAGGAAPITRVLSNDPFLIMKALDAGSWGVIVPLVNSAEDA
                     ARAVSACRYPPQGIRSFGPVLAADVIGSRDPEKLGGEVLCLVMVETREALERVGEIAA
                     TPGLDGVYIGPSDLALSLGLTPTLEIMEGEHAEAVERIREACHRNGIAAGIHSPSGEW
                     ARRHAEAGFDLITVASDAPLLRMVAGQEMAAAREGLAYTDSRNE"
     gene            complement(1576..>2008)
                     /locus_tag="VFT03_07650"
     CDS             complement(1576..>2008)
                     /locus_tag="VFT03_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEU4848058.1"
                     /translation="ERGAKLLVRWDVEDLERLDGALAEAGVEVAVWRDVEDFREVAGG
                     ADIGLSTAEWAIAETGSLVLESGPGKGRTVTLLPPTYVAVVPVNRVLRTVPEAIERYA
                     GSGGLPANVCFHTGPSRSGDIEMSLAIGVHGPGDVHVLLVG"
BASE COUNT          326 a          704 c          617 g          361 t
ORIGIN      
        1 ctgaggccag cagtctcgtc tcagtgatag taagctctgt cgaagcagga gcgaagggag
       61 ttagaggtgg atgagtcacc cacaacgcct ggtgagggcc cagcgctgcc gggactacgc
      121 ggcgtcgatc acatcggcat caccgtgccc aacatggagc aggcgaccgc cttcttcgtc
      181 gatgtgctcg gctgcgaact gctctacgag cgtgaacctc cgggggatga tgccccacgg
      241 cataggcttg gcgtgccagc aggcagccgc attcagggcg ttcgctttct gcgctgtgcc
      301 aacggcgcaa acatcgagct cttcgaattc tactcacctg accagcgcga ggagttccct
      361 agaccgagcg acgtcgggat acagcacctg gcagtctacg tcgaggacct ggacgccgcc
      421 gtggagcacc tgcgcagcca cgaggtgaac ctcatgtccg gtcccaaccc gttgccagga
      481 ccggaggccg gggagggtaa ccggttcatc tatgccagga cgccgtgggg cctgaccctc
      541 gaactcataa gttaccccgc gccgatggcc taccagcagc gcagcgacgc ccgacgctgg
      601 agaccgccgg ccggtcagta gcacgctgcc ttcggcagct tgtcagcacg ctacgccctt
      661 gcgggcttcg cttgtcagca cggcgcttcg cgccttgtca gcacgcttcg gcccttcggg
      721 cctcgctttt cagcatttca gcttgttgtt ttatgaggtg cggcttctcc cgagttcttg
      781 aaaggattat tcgtttcgag aatcggtata ggcaagccct tcgcgagccg cggccatctc
      841 ctgaccggcg accatcctta ggagcggcgc gtccgatgct accgtgatca ggtcgaagcc
      901 cgcctctgca tgcctcctcg cccactcgcc ggaaggagag tgtatcccgg cggcgatgcc
      961 gtttcggtgg caagcttccc tgatccgctc gacggcctcg gcgtgttcgc cttccattat
     1021 ttcgagggtc ggagtaagcc cgagggagag cgccaggtcg ctgggtccga tgtagacgcc
     1081 gtctaggcct ggcgttgcgg ctatctcgcc gaccctttcg agggcctccc tcgtctcgac
     1141 catgacgagg cagaggacct cccctccgag tttttccgga tctctcgacc cgatcacgtc
     1201 cgcggccagc accggcccga aggagcgtat gccctgtggc gggtagcggc aggcggagac
     1261 ggcgcgggcg gcgtcctcgg cgctgttgac gagcggcacg atcacacccc acgagccggc
     1321 gtcgagggcc ttcatgatca ggaacgggtc gttggagagc acgcgcgtta tgggcgccgc
     1381 acctcccgcc cggatggcct ggaacatcgg cgccatcgag ccgtagtcga tgaccccgtg
     1441 ctgctggtcc acgcacacgt aatccgcccc ggctttggcg aggatctccg cccctgtagt
     1501 gccgggcatt acggaccaca gcccgaacgc cggccggtcc ccggcccaga tctctcttat
     1561 agggttcgta gccatctaac ccaccaggag gacgtgcacg tcccccggac cgtgcacccc
     1621 gatagctagc gacatctcga tgtccccgga gcggctcgga cccgtgtgga agcacacgtt
     1681 ggcgggcagg cccccgctcc ccgcgtacct ctcgatggcc tcgggcaccg tcctcagtac
     1741 tctgttcacg gggaccacag ccacgtaggt cggcggcagg agggtgacgg tgcgcccctt
     1801 gccgggcccg ctctccagga ccaggctacc tgtctccgct atcgcccact ccgccgtgga
     1861 gagccctatg tcggcccctc ccgcgacctc cctgaagtct tcgacgtcac gccacaccgc
     1921 tacctcgacg ccagcctcag ccagagcgcc gtccagccgc tccaggtcct ccacgtccca
     1981 gcggacgagg agcttcgccc cgcgctcc
//
LOCUS       DASTUV010000381         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1040691, whole genome shotgun sequence.
ACCESSION   DASTUV010000381 DASTUV010000000
VERSION     DASTUV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040691"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFT03_07655"
     CDS             complement(<1..755)
                     /locus_tag="VFT03_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU4848059.1"
                     /translation="MTETAEGTLLWEPPGELKDNAIITRYMRWLKENRNLQFEDYGEL
                     WEWSVTDVEGFWSSLWEFLDVKSSKPYERALAKREMPGAEWFPGAELNYAEHVFRNAG
                     ERLDEPALVHKSEIQPLGETSWAQLREKVGALSAALEAMGVERGDRVVAYMPNVPEAV
                     VAFLATASIGAIWSSGSPDFGAGSIVDRFEQIEPKVLFAVDGYRYGGKDYDRTQVVAK
                     LQEEIPTVQKTVVLSYLSDEPDLDTLENAVLWD"
     gene            complement(766..1551)
                     /locus_tag="VFT03_07660"
     CDS             complement(766..1551)
                     /locus_tag="VFT03_07660"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEU4848060.1"
                     /translation="MGEASIAPGTSTVDLDLTGRSALVTGAASGIGRACAGRLARAGA
                     SVTVLDLDGDAAKKVAEGIGGEPLQADLSDYEVLDNLEVDADIIVNNAGLQHVAPIEE
                     FPPERFSLILRIMLEAPFRILQKALPGMYEKGWGRVINISSVHGLRASPYKSAYVSAK
                     HGLEGLSKTLALEGGPRGVTSNCVDPAYVKTPLVEKQIADQARIHGISEDEVIEKIML
                     TEPVIKRLIEPEEVAELTLFLCSKEASFINGASIIMDGGWTAR"
     gene            complement(1629..>2121)
                     /locus_tag="VFT03_07665"
     CDS             complement(1629..>2121)
                     /locus_tag="VFT03_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU4848061.1"
                     /translation="AWEVFDEEVREASRGTRFDEVEEAGKVHRFENLEELAETFGLPA
                     GTLAATVKQANRAARGEARDPFGRLEFSGGPLEPPFYGIQVRGALFHTQGGLKVDTSA
                     RVLRPNGGHVPGLYAGGGTAVGVSGSGYRGYSSGNGLLAATVLGKIAGETASGEVVTG
                     GRL"
BASE COUNT          341 a          750 c          621 g          409 t
ORIGIN      
        1 tcgtcccaca gcacggcgtt ctcgagggtg tcgaggtccg gctcgtcgct aaggtaagag
       61 agtacgacgg tcttctggac ggtggggatc tcctcctgca gcttcgccac tacctgcgtc
      121 ctgtcatagt ccttgccgcc gtagcggtag ccgtccacgg cgaagaggac cttcggctct
      181 atctgctcga agcggtccac gatgctcccc gcgccgaagt ccggagagcc gctcgaccag
      241 attgccccta tggacgctgt tgccaggaag gcgacgacgg cctcggggac gttgggcatg
      301 taggccacga ccctgtcgcc gcgctccacg cccatcgctt caagcgccgc ggagagggct
      361 cccaccttct cgcgcagctg tgcccagctc gtctcgccga ggggctggat ctccgacttg
      421 tgtacgagcg ccggctcgtc gaggcgttcg ccagcgttgc ggaagacgtg ctcggcgtag
      481 ttgagctccg cccctggaaa ccactcggca cccggcatct cccttttcgc gagcgcccgc
      541 tcgtagggct tcgaggactt cacgtcgagg aactcccaga gcgaggacca gaacccctcg
      601 acgtcggtca cggaccattc ccacagctcg ccgtagtcct cgaactggag gttcctgttc
      661 tctttcagcc acctcatgta gcgggtgatg atcgcgttgt ctttcagttc gccggggggc
      721 tcccagagca gggtcccctc tgctgtctcg gtcatgccgt acctcctagc gggccgtcca
      781 cccgccgtcc atgattatcg atgcgccgtt tatgaacgaa gcctccttcg agcacaggaa
      841 gagggtcagc tctgctacct cttccggctc gataaggcgt ttgatcacgg gctccgtgag
      901 catgatcttt tcgatcacct cgtcctcgga gatcccatgg atcctggcct ggtccgctat
      961 ctgcttctcc accagcgggg tcttcacgta cgccgggtct acgcagttgg aggtgacgcc
     1021 tctgggcccg ccctccagag caagggtctt ggagagaccc tccaggccgt gcttggcaga
     1081 gacgtacgct gacttgtaag gcgaggccct caaaccgtgt actgaggaga tgttgatgac
     1141 gcgtccccac cccttctcgt acatccccgg cagcgccttc tggaggatcc tgaacggggc
     1201 ctcgagcata atccgcaata tgagcgagaa gcgctcgggc gggaactcct cgatgggcgc
     1261 gacgtgttgc aacccggcgt tgttcacgat gatgtccgcg tctacttcga ggttgtcgag
     1321 cacctcgtag tcggagaggt ctgcctgcag cggctctccg ccgatgccct ccgcgacctt
     1381 tttcgcggcg tcaccgtcca ggtcgagcac ggtcacggaa gcgccggcgc gggccagcct
     1441 accggcgcag gctctcccga taccgctcgc ggccccggtc accagagcgc tgcgccctgt
     1501 aagatccagg tccaccgtcg aagtccccgg cgctatagaa gcttcaccca tgatcctcgt
     1561 tcacctcgaa gaagccgtct ttatctgcaa cgttctttcc cgaaacaccc cacgagaaac
     1621 aatacaggtt agagccgccc gccagtaacg acctcgcccg atgccgtctc gccggcgatc
     1681 ttgcccagca ccgtggccgc cagcagtccg ttcccgctcg agtagcctct gtacccgctc
     1741 ccactgacgc ccaccgccgt ccccccgccc gcgtacagac ccggtacgtg cccgccgttt
     1801 ggcctgagca ccctggcgga ggtgtccacc ttcaatccgc cctgggtgtg gaacaacgcc
     1861 ccgcgcacct ggatgccgta gaaaggcggc tccagcggtc cgccggagaa ctccaggcgc
     1921 ccgaagggat cccgcgcctc tcccctggcc gcccgattgg cctgcttcac ggtcgccgcc
     1981 aaggtgccgg cgggaagccc gaaggtctct gccagctcct cgagattctc gaagcgatgg
     2041 actttgccgg cctcttcgac ctcgtcgaag cgggtgccgc gcgaggcttc gcgaacctct
     2101 tcgtcgaaga cctcccacgc c
//
LOCUS       DASTUV010000382         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1387062, whole genome shotgun sequence.
ACCESSION   DASTUV010000382 DASTUV010000000
VERSION     DASTUV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387062"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFT03_07670"
     CDS             complement(<1..1194)
                     /locus_tag="VFT03_07670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU4848062.1"
                     /translation="MSELLLGIDVGTSSTKGVLARPEGEVVATAERPHELSLPRPGWA
                     EHDAEKVWWDDFVSICRELLGQSDDGVAAVCTSGIGACLLPADEAGNPLRPAILYGID
                     TRAEREIEELNERYGEENLLERCGSVLTTQAVGPKLLWLRRNEPEVWEKTRKFFMANS
                     FIAWRLTGEYVLDHHSASQCDPLYDVREYRWISEWAEEIAPGLPLPRLLWPAEVVGEV
                     TREASEATGIPAGTPVAAGTIDAWSEGASVGVQDPGDLMLMYGTTMFMIEVLREPLSH
                     PGLWGTTGILPNTHNLAAGMATSGALTGWLKKISGDLPYEKLTEEAAAVPPGSDALVV
                     LPYFAGERTPIFDPRARGLISGLTLSHGRGHLYRAMLEATAYGVRHIFEFMREAGGGG
                     ERLVAV"
     gene            complement(1191..2615)
                     /locus_tag="VFT03_07675"
     CDS             complement(1191..2615)
                     /locus_tag="VFT03_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEU4848063.1"
                     /translation="MAKVGILTFSDGRDFVHEGAGVGAFARDVEDDIVGALKAAGHEV
                     VRARELVWTNRLATSEARRVAAARPDLTVFNIPVWAFPHFSMLAANETQGPLLLFSNI
                     NPEQPGMVGMLAAAGGLDQIGRTYGRAWGDVSDPAVLSRLETHIRAAKAVRSLEGSTF
                     GRIGGRPMGMYTAVSNTDQWMEKFGVDVEEIDQWELVRRSGEVEGSKVRAAREWLEEH
                     AAGVHYDGDRLTPEILERQIRSYYVMRELIEEWNLDFSGIKGQPELTTHFATMDITEA
                     FLNDPYDWDGPKEPHVCATEADMDAALTMQILKHLSGTPVLFADVRHYHANRDVWDLC
                     NSGQHATWFAARSDDPAENMRHVHLYPEEFFFPAGGAAVHHLAAEGDFTFARLTRHNR
                     FYRMQVLRGAFVRFDDETNEDLMSQSTYAWPHAFAKMDAAAEEILSRYGSNHIHAIPG
                     DYVEELRMICDILNMDYDGFGGAA"
     gene            2753..>3700
                     /locus_tag="VFT03_07680"
     CDS             2753..>3700
                     /locus_tag="VFT03_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4848064.1"
                     /translation="MSLRVLLVGGPMYDPLYARLEEFEVRDGVEVEAVVASSHPDLNE
                     RIEEEFGSGAASYDLISTHAKYAPSQKRWLTPLDDDLDSAELEPFATRPLELARIEGT
                     LYGVPRNLDVKLLYHRTDLVPEPPISWEEFRDEAARLRSDNVYGFVFPGKESGLFGHF
                     FELHAMAGGRMFEESGPPAPRLNDEAGRWALALLKNLYERAAPRETPDWHYDEVAACF
                     REGRAAMSTDWPGGFYTYEDPETSAVAGSYDVALYPEGRAGRLIYSGCHTFAIPGTVR
                     DRPAAVELLRFLTSRESQAFEARLGTLPARSDSLGDARSE"
BASE COUNT          577 a         1259 c         1229 g          635 t
ORIGIN      
        1 gaccgccacg agccgctctc ccccgccccc cgcctcgcgc atgaactcga agatgtgcct
       61 cacgccgtag gccgtcgcct cgagcatcgc cctgtataga tgaccacgcc cgtgactcaa
      121 ggttaggccg gagatgagac cgcgggcacg cgggtcgaag atgggggtcc tctccccggc
      181 gaagtagggc agcacgacga gggcgtcaga acccggcggc acggctgcgg cctcctccgt
      241 gagcttctcg tagggcaggt cgcctgagat cttcttgagc cagccggtca gggccccaga
      301 cgtcgccatc ccggccgcca ggttgtgggt gttcggcaag atacccgtcg tgccccagag
      361 gccggggtgg gagagcggct ctcttagcac ctcgatcatg aacatcgtcg tgccgtacat
      421 gagcatcaga tcgcctgggt cctggacccc gacgctcgcg ccctcgctcc aggcgtctat
      481 ggtccccgcc gccacaggag ttccggccgg gataccagta gcctcggacg cctcccgcgt
      541 cacctccccg accacctcgg ccggccacag gagacgcgga agcggcaacc ctggcgctat
      601 ctcctcagcc cactcggaga tccagcggta ctcgcgcacg tcgtagagag gatcgcactg
      661 gctagccgag tggtggtcga gcacgtactc gcccgtgagc cgccaggcga tgaacgagtt
      721 cgccatgaag aacttgcgcg tcttctccca gacctcgggc tcgttgcgcc gcagccagag
      781 gagcttgggg ccgacggcct gggtagtcag caccgagccg caacgttcga ggaggttctc
      841 ctccccgtag cgctcgttga gctcctcgat ctcgcgctct gcccgcgtgt cgatgccgta
      901 gaggatcgcg ggcctgagag ggttgcccgc ctcgtcggcc ggcaagagac acgccccgat
      961 gccgctcgtg cagaccgcgg cgaccccatc gtccgactga cctagcaact cccggcagat
     1021 ggagacgaag tcatcccacc acaccttctc ggcgtcgtgc tcagcccagc cggggcgcgg
     1081 cagcgagagc tcgtgtggcc tctccgcagt agcgaccacc tcgccttcgg gccgggcgag
     1141 cacgcccttc gtgcttgagg tgcctacgtc tatccccagg agtagctcgc tcacgccgct
     1201 ccaccgaaac cgtcgtagtc catgttcaga atatcgcata tcatgcgcag ctcctcgacg
     1261 tagtcgccgg ggatggcgtg gatgtggttg gagccgtagc gggagaggat ctcctccgct
     1321 gccgcgtcca tcttcgcgaa ggcgtggggc caggcgtagg tggactggct catgaggtcc
     1381 tcgttggtct cgtcgtcgaa gcgcacgaac gcgccgcgca gcacctgcat cctgtagaac
     1441 ctgttgtgcc gcgtcaggcg cgcaaaggtg aagtcgccct cagccgccag gtggtgcacc
     1501 gccgcgcctc cggcggggaa gaagaactcc tcggggtaga ggtggacgtg gcgcatgttc
     1561 tcagccgggt cgtcgctgcg cgctgcgaac caggtggcgt gctggccgga gttgcacagg
     1621 tcccagacgt cgcggttcgc gtggtagtga cgaacgtcgg cgaagaggac cggggtgccg
     1681 gagagatgtt tgaggatctg catcgtgagg gctgcgtcca tgtcggcctc ggtggcgcag
     1741 acgtgcggct ccttggggcc gtcccagtcg taggggtcgt tgaggaaggc ttcggttatg
     1801 tccatcgtgg cgaagtgggt ggtcagctcg ggctggcctt ttatgccgga gaagtccagg
     1861 ttccactcct ctatcagctc gcgcatcacg tagtaggacc ggatctggcg ctccaggatc
     1921 tccggcgtga gccggtcccc atcgtagtgc accccggcgg catgctcttc gagccattcc
     1981 ctcgcggcgc gcaccttgct cccttccacc tcgccggagc gcctgaccag ctcccactgg
     2041 tcgatctcct ccacgtccac cccgaacttc tccatccact ggtccgtgtt ggagacggcg
     2101 gtgtacatgc ccatcgggcg cccgccgata cggccgaagg tgctcccctc gagggagcgc
     2161 actgccttcg cggccctgat atgggtctcc aggcgcgaga gcaccgctgg gtcggagacg
     2221 tcgccccagg cgcggccgta ggtgcgtcct atctggtcga gcccgcccgc cgcggcgagc
     2281 atccctacca tcccgggctg ttcggggttg atgttggaga agagcaggag aggcccctgc
     2341 gtctcgttgg ctgccagcat ggagaagtgc gggaaagccc acaccgggat gttgaagacc
     2401 gtcaggtccg gcctcgcggc ggcgacgcgg cgcgcctcgc tggtggcgag ccggttggtc
     2461 cagacgagct cgcgcgcccg cacgacctcg tgccctgcgg ctttgagggc cccgactatg
     2521 tcatcctcaa cgtcgcgggc gaacgctccg acgcctgcgc cttcgtggac gaagtcccgg
     2581 ccatcggaga acgtcagaat tccaaccttt gccacgactt ctcctttccg ctcggcccag
     2641 tagtgttgca cggtccagac cgccgcgcca ccggggaggt gatcgttgac ccggtcgccc
     2701 gtgatcggta catttcgccg aggatgccga cgacgcgggg gcgggtatgg cgatgagctt
     2761 gcgggtcttg ctggtgggtg ggccgatgta cgatcccctg tacgcgcgcc tcgaggagtt
     2821 cgaggtgagg gacggcgtcg aggtcgaggc tgtcgtcgcc tcctcgcatc cggatctcaa
     2881 cgagcggatc gaagaggagt tcggctcggg cgcagcctcg tacgacctga tctccacgca
     2941 cgccaagtac gccccgagcc agaagcgctg gctcactccc ctcgacgacg acctcgacag
     3001 tgcggagctc gaaccgttcg cgacgcgccc cctcgagctc gctcgcattg aggggacact
     3061 ctacggtgtc ccgcgaaacc tggacgtgaa gctgctctac caccgcaccg accttgtccc
     3121 cgagccaccc atctcgtggg aagagttccg ggacgaagcc gcccggctga gatcggacaa
     3181 tgtctacggc tttgtcttcc caggcaaaga gtccgggctc ttcggccact tcttcgagct
     3241 gcacgcgatg gcgggagggc ggatgttcga ggagagcggg ccgccggcgc cgaggctgaa
     3301 cgacgaggcg ggccgctggg ctctcgccct cctgaagaac ctctacgagc gggccgcgcc
     3361 gcgggagacg ccggactggc actacgacga ggtggccgcc tgcttcaggg agggcagggc
     3421 cgcgatgtcc acagactggc ccggcggctt ctacacctac gaggaccccg agacgagcgc
     3481 cgttgcgggc tcctacgacg tcgcgctcta ccccgaggga agggcggggc gcctcatcta
     3541 ctcgggctgc cacaccttcg cgatacccgg tactgtccgc gatcggcccg ccgcggtcga
     3601 gctgctgcgt ttcctcactt ctcgcgagtc gcaggcgttc gaggccaggc ttggaacgct
     3661 cccggcgcgc agcgactctc taggggacgc ccggtccgaa
//
LOCUS       DASTUV010000383         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1726437, whole genome shotgun sequence.
ACCESSION   DASTUV010000383 DASTUV010000000
VERSION     DASTUV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726437"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFT03_07685"
     CDS             <1..692
                     /locus_tag="VFT03_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU4848065.1"
                     /translation="NRWRGYEEWPVPGAYEERWHLHGGGCLAREAPERGEPDEYDYDP
                     RDPVPTVGGAILMAPIHRPGARDQREIEDRPDVLVYTSEALRKDHTVLGPVHATVFAA
                     TSAPDTDFVVRLVDVYPDGRAIGVADGVIRASAREAFPAPGVTRPAGPKPIEPGRVYS
                     YNVDLWATGITFRAGHRIGVHITSSCFPRWDRNPNTGEGPDSAHTEVARQRIFHDPER
                     PSHVTLTIVDR"
     gene            731..1477
                     /locus_tag="VFT03_07690"
     CDS             731..1477
                     /locus_tag="VFT03_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4848066.1"
                     /translation="MEIEGRTFLVAGGGSGLGGATTEMLTNEGANAVVADLRGEAAGG
                     NVRFVETDVTDEASVRRAIEEALQSFGGMHGAINCAGIASAEKVLGREGPHSLDSFAK
                     VVEVTLVGTFNVLRLAAEVMVNNEPAASGERGVIVNTASVAAYDGQIGQVAYAASKGG
                     VVALTLPVARELAQYGIRVVAIAPGIFDTPMMAGLPEAARESLGKQVPFPSRLGRPEE
                     YAALVRHIIENEMLNGEVIRLDGSIRMAPR"
     gene            1500..1649
                     /locus_tag="VFT03_07695"
     CDS             1500..1649
                     /locus_tag="VFT03_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848067.1"
                     /translation="MAALRIVTVHVTVYGGVTRPPSQHAQSVFGVGRPERPDLQPVEP
                     LAARC"
     gene            complement(1835..2590)
                     /locus_tag="VFT03_07700"
     CDS             complement(1835..2590)
                     /locus_tag="VFT03_07700"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEU4848068.1"
                     /translation="MNPLISQYADALLRAGVAGTNTSHGAEDNLYKIDRLLDGEPSQT
                     FGMDELLEDVSFEEAYEAVTRQTGHPPDMEQTGGRGCIDPARTAAGLVEAGERVRAAA
                     EAGGTFVIATGHPGALLSYYLGLAAWVEELGGETLTGRTQGRYERGSILDWVGAVGSL
                     GDGASLRHTHDADPMRDLMRELDGIDLVVADHGFAGAAIIANIPTVAVMDTNDPALAV
                     VARRGADVTVVPMDDNRSQGSYAAALQVLKGKP"
     gene            complement(2601..3590)
                     /locus_tag="VFT03_07705"
     CDS             complement(2601..3590)
                     /locus_tag="VFT03_07705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU4848069.1"
                     /translation="MATLALTGDVMLGRGVNEALRAARPAEPWGDVLPLLLSADLRLI
                     NLECAITVQRRPWLRTRKVFHFRADPPAVEVLEAARVDACSLANNHTLDFEEKGLLDT
                     LEHLEAAGIRYAGAGRNLEEAARPVLLEGGLALVAFTDNEPPFAAGPDRPGTNYLPVS
                     TEPEVMRRVEEAIGAARDAGAGTVVFSNHWGPNMVRRPPEAFRRFARAVVDRGADVYY
                     GHSAHVFQGVEVYRGRPILYDTGDFIDDYAVDPDLRNDHSFLFRLSTEDGELRRLELF
                     PVVLPYARVELAAGEEREAILDIMQDLSAEMGTTFERREDRLVLEPSPVPDLC"
     gene            complement(3648..3782)
                     /locus_tag="VFT03_07710"
     CDS             complement(3648..3782)
                     /locus_tag="VFT03_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU4848070.1"
                     /translation="MLETTTEAGGRADRRLREEKIAWLTTVRSDGQPQSVPRQAVVVR
                     "
     gene            complement(3839..4237)
                     /locus_tag="VFT03_07715"
     CDS             complement(3839..4237)
                     /locus_tag="VFT03_07715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU4848071.1"
                     /translation="MIHETKLRVRYAEIDAQGHVNNAVYLSYFEVGRVEWLRAAGLSY
                     RELEAQGYGIVVVEVLAHYRRAAFFDDELTLRTELADLSRASMRFEYEVSRDGELLVT
                     GHTRHACVDLATGKPIRVPEEVLAAARKAS"
     gene            complement(4234..4359)
                     /locus_tag="VFT03_07720"
     CDS             complement(4234..4359)
                     /locus_tag="VFT03_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848072.1"
                     /translation="MDLVSGLEYEADQFALLFATEDAREGMGAFVEKRKPEFRGQ"
BASE COUNT          693 a         1498 c         1492 g          721 t
ORIGIN      
        1 agaaccgctg gcgcggctac gaggagtggc ccgtccccgg cgcgtacgaa gaacgatggc
       61 acctgcacgg cggcgggtgc ctggcccgcg aagcgcccga gcggggcgag ccggacgagt
      121 acgactacga cccgagggac ccagtcccca ccgtgggcgg ggcgatactc atggcgccca
      181 tccaccgtcc cggcgcccgc gaccagcgcg agatcgagga ccgccccgac gtgctcgtct
      241 acacgagcga ggctctcagg aaagaccaca cggtcctcgg tcccgtacac gcaaccgtct
      301 tcgcggcaac ctcagcccct gacacggact tcgtcgtccg gctcgtcgac gtctacccgg
      361 acgggcgggc gatcggggtg gcggacggcg tgatccgggc cagcgcccgc gaggcttttc
      421 ccgctcccgg cgtcacgaga ccagccgggc caaagcccat cgagccggga cgcgtataca
      481 gttacaacgt ggacctgtgg gcgacaggaa tcaccttcag ggcagggcac cgcatcgggg
      541 tgcacatcac ctcgagttgc ttcccgcgct gggaccgcaa cccgaatacc ggcgagggtc
      601 ccgactcggc gcacaccgag gtggctcggc agaggatctt ccacgacccc gagcgcccga
      661 gccacgtcac gctgaccatc gtcgacagat agaggaggat catgaacgga tgtttccctg
      721 aggtcgccgg atggagatag agggacgcac cttcctcgtc gccggtggcg gctcgggtct
      781 cggcggcgcc accaccgaaa tgctcacgaa cgagggcgcg aacgccgtgg tggcggacct
      841 cagaggcgag gccgcaggcg gcaacgtccg ctttgtcgag acggacgtca ccgacgaggc
      901 gagcgtgagg cgggccatcg aagaggcttt gcagagcttc gggggcatgc acggcgcgat
      961 caactgcgcc ggcatcgcgt cggctgagaa ggtgctgggc agggagggtc ctcactctct
     1021 ggattccttc gcgaaggtag tagaggtaac cctcgtgggc acgttcaacg ttctcaggct
     1081 cgcggccgaa gtcatggtga acaacgagcc cgccgctagt ggcgagcgcg gggtcatcgt
     1141 caacaccgcg tcggtagccg cgtacgacgg ccagatcggg caggtcgcct acgcagcctc
     1201 caaggggggc gtcgtcgcgc tgacgctgcc cgtcgcccgc gagctcgcgc agtacggcat
     1261 cagggtggtt gccatcgccc cgggtatctt cgacaccccg atgatggccg ggcttccaga
     1321 ggcagcgcgc gagtcgctgg gcaaacaggt tcctttccca tcgcgcctgg gccgtccgga
     1381 agagtacgcg gcgctcgtaa ggcacatcat cgagaacgag atgctcaacg gcgaggtgat
     1441 acggctggac ggctcgatcc ggatggcccc gcgctgacgc gaccccggcg gagcctctca
     1501 tggcggcgct ccgcatcgtc accgtacacg tcaccgtgta cgggggtgtc acgcgtcccc
     1561 cgagccagca cgcccagagc gtcttcggcg tcggccggcc cgagcgcccc gatctccaac
     1621 ccgtcgagcc actcgccgcc cggtgttgac ttcaaggtct cggtcacgtc ttcctctctt
     1681 tctccccgcc tacgttcacg gctgaataga agcgccgtca aggaggtttc ccccgatatg
     1741 accggagagc aggccatctc gttcggagta cgcctgcggc ggctgaggga ggccgccggg
     1801 gtcactcagg agccgtgacc cgaggttgtt gggatcatgg cttgcccttg agcacctgga
     1861 gcgcggcggc gtaggaaccc tgcgagcggt tgtcgtccat gggcacgacc gtcacgtccg
     1921 cgccgcgccg ggccaccacc gcgagggccg ggtcgttggt gtccatgact gctaccgttg
     1981 ggatgttggc tatgatggcc gcgccggcga acccgtggtc cgcaaccacg aggtctatgc
     2041 cgtccagctc ccgcatcagg tcccgcatag ggtcggcgtc gtgggtgtgc cgcaggctgg
     2101 caccgtctcc cagagatccc accgccccca cccagtcgag gatggagcct ctctcgtagc
     2161 gaccctgcgt ccgcccggtg agcgtctccc cacccagctc ctcgacccac gccgcgaggc
     2221 ccaggtaata agagagcagg gcgccgggat gtccggtcgc gatgacgaaa gtgcctccgg
     2281 cctctgcggc ggcccggact cgctctccgg cctctacgag cccggcggcg gttcgcgccg
     2341 ggtctatgca cccgcgcccg cctgtttgct ccatgtcagg ggggtgaccg gtctggcggg
     2401 tcactgcctc gtaggcctcc tcgaagctta cgtcctcgag tagctcatcc atgccgaaag
     2461 tctggctggg ctcgccatcg aggagacggt ctatcttgta gaggttgtcc tccgccccgt
     2521 gggaggtgtt ggtcccggcg acgccggccc tgagcaaggc gtccgcgtac tggctgatca
     2581 gagggttcac gagggcgagt ttagcagagg tccggtaccg gggagggctc gagtaccaga
     2641 cggtcctcgc gccgctcgaa ggtggtgccc atctctgcgg agaggtcttg catgatatcg
     2701 aggatcgcct cccgctcctc gcccgcggcc agctccaccc tggcgtaggg caggacgacg
     2761 gggaacagct ccaggcgcct gagctctccg tcctccgtag agaggcggaa caggaaggag
     2821 tggtcgttgc gcaggtcagg gtccacggcg tagtcgtcta tgaagtcgcc cgtgtcgtac
     2881 aggatcggcc tgccgcggta gacctccacc ccctggaaga cgtgcgcgct gtgcccgtag
     2941 tagacgtcgg ctcccctgtc aacgacggcg cgggcgaacc gccggaaggc ctctgggggc
     3001 cgccgcacca tgttcggccc ccagtggttc gagaacacca ccgtcccggc tccggcatcg
     3061 cgcgcggctc cgatagcctc ctccaccctg cgcatgacct ccggctccgt agagaccggc
     3121 aggtagttgg tgcccggcct gtccgggccg gcggcgaagg ggggctcgtt gtccgtgaac
     3181 gccactagcg cgagcccacc ctcgaggagg acaggccgcg cggcctcctc caggttgcgt
     3241 ccggccccag cgtaccggat gccagcggct tcgaggtgtt ccagggtgtc gagcaagccc
     3301 ttctcctcga agtcgagggt gtgattgttg gccagcgaac aggcgtccac gcgcgccgcc
     3361 tcgagaacct cgactgccgg cgggtcggcg cgaaagtgga agaccttgcg ggtgcgcaac
     3421 cagggccgtc tttggacggt tatggcgcac tccaggttga tgaggcgcag gtcggcggag
     3481 aggagcagcg gcagcacgtc accccacggt tccgcgggtc gggcggctct gagcgcctca
     3541 ttcaccccac gcccgagcat cacgtcgccg gtgagagcca gcgtagccat tctcatcaag
     3601 cgtattgaag caccgcggcc gctcttgcgc ctcctgaggc ttggcggcta cctcaccact
     3661 acggcttgcc gtggcaccga ctgcggctgc ccgtccgagc gtaccgtggt gagccaggcg
     3721 atcttctcct cccgcaaccg cctgtctgcg cggcctcccg cctcggtagt ggtctcgagc
     3781 atgctcgagc tcctttcatt gctcccggag agcttaaccg aggtacgccg tcgtcgtcct
     3841 aggatgcttt acgggctgcg gcgaggacct cctctggcac ccttatcggt ttgcctgtgg
     3901 cgaggtccac gcacgcgtgg cgcgtgtggc ctgtgacgag caactctccg tcacgcgaga
     3961 cttcgtattc gaagcgcatg gaggcccggc tcaggtccgc cagctcggtg cgcagcgtga
     4021 gctcgtcgtc gaagaaggcg gctctgcggt agtgggcgag cacctccacg acgacgatac
     4081 cgtagccctg cgcctcaagc tccctgtagg agagaccggc ggcgcgcagc cactcgacgc
     4141 ggccgacctc gaagtacgag aggtagactg cgttgttgac gtgcccctga gcgtcgatct
     4201 ccgcgtagcg gacacggagc ttggtctcgt ggatcactgg cctctgaact cgggcttgcg
     4261 cttctcgacg aaggcgccca tgccctcgcg cgcgtcttcg gtggcgaaga ggagggcgaa
     4321 ctggtcggcc tcgtactcga gcccgctcac caggtccacg tcggtcgagc ggttcgccgc
     4381 ggccttcgcg tggcgtacgg cgag
//
LOCUS       DASTUV010000384         4043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1235655, whole genome shotgun sequence.
ACCESSION   DASTUV010000384 DASTUV010000000
VERSION     DASTUV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235655"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFT03_07725"
     CDS             <1..231
                     /locus_tag="VFT03_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848073.1"
                     /translation="GDHTQMGWEVKDIEATVEQLRARGVEFEEYDFPGLRTVNSIAEI
                     EGNYPSKGGVGERAAWFRDVDGNLIGIGQPMR"
     gene            347..541
                     /locus_tag="VFT03_07730"
     CDS             347..541
                     /locus_tag="VFT03_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4848074.1"
                     /translation="MRYMLMVCYDESFSPTETIGAETKEWIAGYDIIEYEDLDEAVEV
                     VSGHPMAKIGTIEIRPCPEA"
     gene            526..1791
                     /locus_tag="VFT03_07735"
     CDS             526..1791
                     /locus_tag="VFT03_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4848075.1"
                     /translation="MPGSVTGEGVEAAVAGAFREEWGRLVATLIRTTGDWDLAEECAQ
                     GAFERALKTWPRDGVPRRPGAWLTTVARNLARDRLRRKATGAAKLEEVALMSRSDDAD
                     RDAGDESGIHDDRLRLIFTCCHPALPLEARVALTLRTLAGLTTAEIARAFLVSEPTMA
                     KRLVRAKGKIRNAAIPYRVPPAHLLPERTGGVLAVLYLLFNEGYAATSGADLVRERLS
                     AEAIRLARTLVQLMPDEPEALGLLALMLLHDARRAARVEGAGDLVTLEEQDRDRWDQD
                     EIGEGVRLLDGPLRLAEPGPYQLQAAIAACHATAADVTDTDWAEIAALYGRLARMVPS
                     GVVKLNRAVAVGMADGPAAGLALVEEIEAAGKLAGYHLLPATRADLLRRLGRQTEAAI
                     AYREAFELAATEAEHRYLARRLEETEREE"
     gene            1795..2073
                     /locus_tag="VFT03_07740"
     CDS             1795..2073
                     /locus_tag="VFT03_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4848076.1"
                     /translation="MTDNNARGTLINQVGAVFVPVADQDQALGFYLDTLGFEKRADFT
                     YGEDRRWMEVAPPGAANTLADSSVAEGPRTLQVVWCIAAGLERVQYYE"
     gene            2105..2287
                     /locus_tag="VFT03_07745"
     CDS             2105..2287
                     /locus_tag="VFT03_07745"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU4848077.1"
                     /translation="MPTRSSREDKNEAKMDRAKGRMKEAGGALTGDNSKKREGRSDQR
                     KAKAKEKKGNLKDLLS"
     gene            2485..3096
                     /locus_tag="VFT03_07750"
     CDS             2485..3096
                     /locus_tag="VFT03_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848078.1"
                     /translation="MSEERSRRERDDSVPARRDDTEERRTTRKEQDVLLDVSELEVDR
                     ITLEVAGLRAHVSVLAQLASLVNLQVGVDARLNRVKLEIEGVRAKVLLKVWLDDVRAI
                     LEKALDTLGEHPEILESLVKAINELLQGRLGDALGGVESVLEGLEEGDTVDALLKGRL
                     EDVRAALEQVLEEVGVDEGESRVAIGEGPSNSTAQPTQEEEER"
     gene            complement(3284..3502)
                     /locus_tag="VFT03_07755"
     CDS             complement(3284..3502)
                     /locus_tag="VFT03_07755"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEU4848079.1"
                     /translation="MSLGNLGDLGNLDLGQLQQYLPNLDFPASKEEVVSTAQSNDAPQ
                     EVVDRIRNSGDDTFNSADEVLQAVQGKL"
     gene            3691..3945
                     /locus_tag="VFT03_07760"
     CDS             3691..3945
                     /locus_tag="VFT03_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848080.1"
                     /translation="MATDKGKDVVGAAQNAVRQTTGALKGEGPWYSANSDVYHNNPNC
                     QTGNSMDPENVRQGTGGKPLCGECERLNNAGGPVGNITGL"
BASE COUNT          804 a         1198 c         1403 g          638 t
ORIGIN      
        1 ggcgatcaca cccagatggg gtgggaggtc aaagacatcg aggcgaccgt ggagcagctg
       61 cgagcgcgtg gagtggagtt cgaggaatac gactttccgg gtctaaggac tgtcaacagc
      121 atagccgaga tcgaagggaa ctacccgagc aagggtgggg tcggcgagag ggccgcgtgg
      181 ttcagggacg tcgatggcaa cctcattggg atcggccaac cgatgcgttg acgcgggcca
      241 cggtgggccc tgatcggtag gataaatcgt cgagatcagg agcgacctgt ccatctgtgc
      301 catccccgtt ggtcgttgta gtgtaacgac ccgcaaagga ggagagatgc gttacatgct
      361 catggtttgc tacgacgaat ccttcagccc cacggagacc atcggcgccg agaccaaaga
      421 gtggatcgcc ggctacgaca taatcgagta cgaagacctc gacgaggccg tcgaggtcgt
      481 gtccgggcat cccatggcga agatcgggac gatcgagatc cgaccgtgcc cggaagcgtg
      541 acgggcgagg gcgtcgaggc ggcggtcgcc ggggccttcc gggaggagtg ggggcgcctc
      601 gtcgcgaccc tgatccggac gaccggggac tgggacctcg ccgaggagtg cgcccagggt
      661 gccttcgagc gggcgctcaa aacctggcca cgcgacggcg tcccgcgacg ccccggcgcg
      721 tggctgacca ccgtggcacg caacctggcc cgcgaccggt tgaggcgcaa ggcgaccggg
      781 gcagccaagc tggaggaggt ggcgttgatg tcccgatccg acgacgcgga ccgcgacgcg
      841 ggcgacgaga gcggcatcca cgatgatcgg ttgcgcctta tcttcacctg ctgccacccg
      901 gcgctgccgc tggaggcccg cgtcgcccta acgctcagga ccctcgccgg cctcacgacc
      961 gcggagatcg cccgcgcgtt cctggtctcg gagccaacga tggccaagcg gctcgtccgg
     1021 gcgaagggca agatccgcaa cgctgccatc ccgtaccggg ttccgccggc ccacctgctg
     1081 cccgagcgca ctggaggggt gctggccgtc ctgtacctgc tcttcaacga gggctacgcg
     1141 gccacctcag gcgccgacct cgtgcgggag cgcctgagcg ccgaggcgat ccgcctggcc
     1201 cgaacgctcg tccagctcat gcccgacgag cccgaggcgt tgggcctgct cgccctcatg
     1261 cttctccacg acgctcgtcg cgcggcccgt gtcgaggggg cgggcgacct ggtgacgctg
     1321 gaggagcagg accgtgaccg ctgggaccaa gacgagattg gtgagggggt tcgtctcctc
     1381 gacgggcccc tgcggctcgc ggagcccggc ccctaccagc tccaggcggc gatcgccgcc
     1441 tgccacgcca ccgcagccga cgtgaccgac accgactggg ccgagatcgc cgccctctac
     1501 gggcggctgg cgcggatggt cccctcgggg gtcgtgaagc tgaaccgggc agtcgcggtg
     1561 gggatggctg acgggccggc cgcggggctt gcgctggtgg aggagatcga agcggcgggg
     1621 aagcttgccg gctaccacct gttgcccgcc acgcgcgccg atctgctccg ccgcctgggg
     1681 cgtcaaaccg aagcggccat cgcctaccgc gaggcgttcg agctcgcggc gacggaggcc
     1741 gagcatcggt acctggcccg ccggctggaa gagaccgaaa gagaggagtg acgcatgacc
     1801 gacaacaacg cacgcgggac cctgatcaat caggtcggag ccgtgttcgt accggtggcc
     1861 gaccaggacc aggcgctcgg gttctacctc gacacgctcg gtttcgagaa gcgcgccgac
     1921 ttcacctacg gcgaggatag gcgctggatg gaggttgccc cgccgggcgc ggcgaacacc
     1981 cttgccgact cgtcggttgc agagggccca cgcaccctcc aggtcgtgtg gtgtatcgct
     2041 gccgggcttg agcgcgtcca atactatgag tagagaatga cggcgaggag aggagggtat
     2101 ccccgtgccc accaggagca gcagggaaga caagaacgag gcgaagatgg acagggccaa
     2161 gggccgcatg aaggaagcag gaggcgccct gacaggcgac aacagcaaga agagggaagg
     2221 ccgctcggac cagaggaagg ccaaggctaa ggagaagaag ggcaacctca aggacttgct
     2281 gagctagcgc cagctgcggg cactcccaag cgtagcgaag ggtccgaacc ccgcagagtc
     2341 gcgtcccttc gctaagcaga agatcacgta gaagtggttt tctcatagaa gttcgcgtta
     2401 ggcttaggcc ggtgacacca tgcgcatggc gggaaagggc ttcctaccgg cgaagaagtg
     2461 gccggtagga gaaggaggca aaccgtgtca gaggaacgtt cacgacgcga gcgcgacgac
     2521 agcgtaccgg cacgaagaga cgacaccgag gaaagacgta ccacccgcaa agagcaggac
     2581 gtgctcctcg acgtctcaga gctcgaggta gataggatca ccctggaggt cgcggggctc
     2641 agggctcacg tctcggtcct cgcacagctg gccagcctcg tgaacctgca ggtaggggtc
     2701 gatgcaagac tcaaccgggt caagctggag atcgagggag taagggcgaa ggtgctcttg
     2761 aaggtgtggc tagacgatgt ccgtgccatc ctcgagaagg cgctcgacac cctcggcgaa
     2821 caccctgaga tcttggagag cctggttaag gccatcaatg agctgttgca gggcaggttg
     2881 ggggacgcct tgggcggagt ggagagtgtc ttggagggct tggaggaggg ggatacagtg
     2941 gacgcattgc tcaaggggag gctcgaggac gtgcgagcgg ccctcgagca ggtattggaa
     3001 gaggtaggcg ttgatgaggg ggaaagccgc gtggccatag gggagggtcc ttccaactcc
     3061 acggcgcaac cgacgcagga ggaggaagag cgctaaggag agacccccgg ttacggttgt
     3121 tcttctacca cttcctcgag ggtccctagc acctcgtagg agaagcgcaa ctttgccgct
     3181 gttctgacct ctgacctcga atcagggtcc tacaccaggc gcgagaaaag gccgggctca
     3241 agtgccccgg ccaaagacgg aaatgctgct tactatgaag tgcttacagc ttaccctgta
     3301 ccgcctgcaa cacctcgtcg gcgctgttga aggtgtcgtc gccggagttc cttatgcggt
     3361 cgaccacctc ttggggggcg tcgttgctct gggcggtgga gactacctct tccttgctgg
     3421 ccgggaagtc caggtttggt agatactgct ggagttggcc gaggtccagg ttgcccaggt
     3481 cgccaaggtt gcccaagctc atgttcttcc tcccgttcta tttcctacaa cgtcggtgcc
     3541 cttcctaccc ggtttgcccg gtgccaaacg tccgcggcgc cgaacacacg ctttatgtcg
     3601 gtcatcgagg tagagttgtc aaagaggttt aaacttggtg acatgtggtt acatattgaa
     3661 ctataggaga tcgaccggag gaggaaaagc atggccacgg acaagggtaa agacgttgtg
     3721 ggcgcggccc agaacgcggt gaggcagacc accggtgcct tgaagggaga gggcccgtgg
     3781 tactcggcca actccgacgt ttaccataac aaccccaact gccagaccgg caacagcatg
     3841 gaccccgaga acgtccgaca agggacgggt ggcaagcctc tttgtgggga gtgcgagcgg
     3901 ctcaacaacg ctggcggccc cgttggcaac ataaccggcc tctagtaggc aaaagagata
     3961 gctggaggcc aggccctagt ttttgccggg gcccggcctt cgtcaatgct tttatgctcc
     4021 aacgtcgtga tcgacgaacc aac
//
LOCUS       DASTUV010000385         4806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1025289, whole genome shotgun sequence.
ACCESSION   DASTUV010000385 DASTUV010000000
VERSION     DASTUV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025289"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VFT03_07765"
     CDS             <1..1456
                     /locus_tag="VFT03_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4848081.1"
                     /translation="AFLPQHVEGDGRTPLEVVRAARPELAEVRGEIADCEARLCAPEV
                     ADDLRKMQRLLERHGLLLQRFTELGGQGFEGEARGYLRSLGLDGDDADRPTSDLSGGQ
                     RKLAALAACLARRPDVLLLDEPESHLDAVRRERLEALIQTFDGAVVVVSHDRYLLDET
                     VTEIAELDGGKTKSWPGNYSAYALARELALKRQQQLYVTQQKEIARLEEAIRRFQQWA
                     HNTEDERHARQARVKQRQIERMDKIDRPVLERRRIGLGFRENVRGGKKVLELREASVA
                     FGEEPVLIGVDLTVARGERVGVIGANGAGKSVLAKLLAGIIPPTEGERWVGPSIKTGY
                     LAQDPEPPASATPLGLVRDAKPLYEGEAVNLLGRFLFRYEQVREPVRNLSGGERTRLQ
                     LLLLMLGEPNCLILDEPTNHLDIDSLEVLERELERFPGTVVFVSHDRYFLDRISDKTV
                     EVRDAGLYRYEGGYSAWRHSKAGQRGRISASAAG"
     gene            1946..2104
                     /locus_tag="VFT03_07770"
     CDS             1946..2104
                     /locus_tag="VFT03_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEU4848082.1"
                     /translation="MDLLRILIAILLPPLGVFLQVGIGKQFWINVLLTILGYIPGIVH
                     AVWVIAKY"
     gene            complement(2111..2551)
                     /gene="dtd"
                     /locus_tag="VFT03_07775"
     CDS             complement(2111..2551)
                     /gene="dtd"
                     /locus_tag="VFT03_07775"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU4848083.1"
                     /translation="MRIVLQRVKNASVTVGDERISEIGAGLLLLVGVASGDGEAEADW
                     LARKISGLRIFNDREGKMNLSVQDAGGEVLAVSQFTLLADTRKGKRPSFVGAASPEEA
                     ERLFDYFCERLRAAGAGPVKTGAFGAMMDVALVNDGPVTIFLER"
     gene            complement(2552..2938)
                     /locus_tag="VFT03_07780"
     CDS             complement(2552..2938)
                     /locus_tag="VFT03_07780"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEU4848084.1"
                     /translation="MEPSFARLFTVEEANALLPKLLELLEDVARHRDALRERAPHMEP
                     IIRSAGANGGGKAGSEYGVEAYNLYLAVERIRELGIILKDLDAGLLDFPHEREGRVVF
                     LCWHPPEERVAFWHEIEAGYAGRQPI"
     gene            complement(2955..3674)
                     /locus_tag="VFT03_07785"
     CDS             complement(2955..3674)
                     /locus_tag="VFT03_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU4848085.1"
                     /translation="MYAVISDIHGNLEALEAVLRDIPEEVRIVYCLGDVIGYGANPDE
                     CCDIVRELGMPVISGNHDLAVTDLETDLNWFNPVAAAAVVWTREHLSKRNADFLRTRP
                     RMIETRDALFVHGSVRDPDEYIINSAAAEDNLEILRSEYPGVPICFFGHTHVKTVAPS
                     PNGPVIEAHTLDLSSNGPYLVNPGSVGQPRDGDTFASYVLVEGERVTYRFVEYDIGKA
                     QAKIRSAGLPGMLADRLAVGR"
     gene            3778..3850
                     /locus_tag="VFT03_07790"
     tRNA            3778..3850
                     /locus_tag="VFT03_07790"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3811..3813,aa:Arg,seq:ccg)
     gene            4150..4791
                     /locus_tag="VFT03_07795"
     CDS             4150..4791
                     /locus_tag="VFT03_07795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848086.1"
                     /translation="MIRHAFPALLKECSGSLEGLRVLDLGCNSGGFSVEAHKRGAEYV
                     LGVDVVDRYLEQAAFVKTALDIGDSVEFRNVGVYDLDPDEVGRFDVTFFFGLLYHLEN
                     PVLAMRKVSAVTDHVLVVDTNTILTGDKQETPLWRMSFPSPVTSPDDPLASTGLWRNR
                     EYCELKPNAAAVTGLLKLGGFDRVRLLEPTADGLPEVYQNGHRKTFFAVKETA"
BASE COUNT          875 a         1451 c         1589 g          891 t
ORIGIN      
        1 cgcctttctc ccccagcacg tcgagggcga cggtcgcacg ccgctcgagg tggtccgcgc
       61 ggcgcgcccc gagctggcgg aggtaagagg agagatcgcg gactgcgagg ccagactctg
      121 cgcgcccgag gtggcggacg acctgcgaaa gatgcagcgc ttgctggagc gccacggact
      181 gctattgcag cgattcaccg agctgggcgg acagggcttc gagggcgagg cgcgcggcta
      241 cctgcggtcg ctggggctgg atggggatga tgcggaccgg ccgacgtcgg acctcagcgg
      301 cgggcagcgc aagctcgccg cgctcgcggc ctgcctcgcg cggcggcccg acgtactgtt
      361 gctggacgag ccggagtcac acctggatgc ggtgcgccgg gagcggcttg aggctctgat
      421 ccagaccttc gacggggcgg tcgtcgtcgt ctcccacgac cgctacctgc tggacgagac
      481 cgtcacggag atcgccgagc tggatggcgg gaagacgaag tcgtggccgg gcaactactc
      541 ggcgtacgcg ctcgcgcgcg agctcgcgct caagcgccag cagcaactct acgttaccca
      601 gcagaaggag atagcgcgcc tcgaagaggc gatccggcgc ttccagcagt gggcgcacaa
      661 caccgaggac gagagacatg cccgccaggc cagggtcaag cagcgccaga tcgagcgcat
      721 ggacaagatc gaccgccctg tgttggagcg gcgcaggatc ggcctgggct tccgcgagaa
      781 cgtcaggggc ggcaagaagg tgctggagct tcgggaggcg agcgtggcct tcggcgagga
      841 gccggttctt atcggcgtgg atctcacggt cgcgcgcggc gagcgggtcg gcgtgatcgg
      901 ggctaacggc gcggggaaga gcgtcctggc gaagctcctc gccggcatca tcccgccgac
      961 ggagggggag cgctgggtgg gaccctccat aaagacaggg tacctcgctc aggaccccga
     1021 gccgccggcg agcgcgacgc ccctcggcct ggtgcgggac gcgaagccgc tctacgaggg
     1081 cgaggcggtg aacctgctcg gccgctttct cttccgctac gagcaggtgc gcgagccggt
     1141 gcggaacctg agcgggggcg agcgcacgcg cctgcagctc ctccttctga tgctcgggga
     1201 gcccaactgc ctcatcctgg acgagccgac gaaccacctg gacatagact cgctagaggt
     1261 cctcgagagg gagctggagc gattccccgg cactgtcgtc ttcgtctccc acgaccgcta
     1321 cttcctcgac cgcatctcgg acaagacggt cgaggtgcgc gacgccgggt tgtaccgtta
     1381 cgagggcggc tacagcgcct ggaggcactc gaaagcgggc cagcggggac gtatctcagc
     1441 gagcgccgca ggctagatgg cgatttgacg aaggaagata tgtgcgatat acacatagct
     1501 tagcccggtg tcctactcct tcctttccca cctatcaccc ctttggatgg gcaccgggtt
     1561 ccttctcttc gaacccacat gtggggttgc taggctccgg gaactgccac ttgttgtgca
     1621 gtcggctgaa gcttcttgcc cgccgaagta gtgtttgtta taacgtccgt tctaaagtta
     1681 ggaggggaac ctgtgccggt actgctgttc ttcgtatttg tcgccttggt gagtatctgt
     1741 accctggcgt taggataccg gctggcttcg atcagggaac gaaccacgag ccgcgaatgc
     1801 cgcaggtatc gcgggaggaa ggttcgaagg caaagggtat ggtgcaggct agaaagttag
     1861 tcactttgtt cgaggcgtgg agcagcaacc attgggagga aaaagcgtaa taggaagata
     1921 gtagtttgct atacggaggc tttcgatgga tctactacgc atcctgatcg ctatacttct
     1981 cccgcctcta ggcgtgttct tgcaggttgg gatcggcaag cagttctgga tcaacgtgtt
     2041 gctaacgata ctgggttaca ttccgggtat agtccacgcc gtgtgggtta tcgccaaata
     2101 ctgaaccctg ctacctctcc aggaatatcg tgacggggcc gtcgttcacc agcgccacgt
     2161 ccatcatcgc gccgaaggcg ccggtcttca ctggaccggc gccggccgcg cggagcctct
     2221 cgcagaagta gtcgaagagc cgttcggcct cctccggcga ggccgcgccg acgaagctcg
     2281 gacgcttgcc cttcctggta tcggcgagca gggtgaactg cgagacggcg aggacctcgc
     2341 ccccggcgtc ctgcacgctc aggttcatct tcccttcacg gtcgttgaat atcctgaggc
     2401 ccgatatctt ccgcgcgagc cagtccgcct cggcctcccc gtcgcctgag gcgaccccga
     2461 cgaggagcag gaggccggct cctatctcgg agatcctctc gtcgccgacg gtgacggaag
     2521 cgttcttcac ccgctggagt actatccgca tctagatcgg ctgtcggccg gcgtagcccg
     2581 cctctatctc gtgccagaag gcgacccgct cctcgggcgg gtgccagcac agaaagacga
     2641 cgcgcccttc gcgctcgtgc gggaagtcga ggaggcccgc gtcgagatcc ttgaggatga
     2701 tgccgagctc cctgatacgc tccaccgcca gatagaggtt gtaagcctcg acgccgtact
     2761 cagagcccgc ctttccgccg ccgttcgcgc cggcggagcg gatgatcggc tccatgtgcg
     2821 gggctctctc gcgcagggcg tcgcggtggc gcgcgacatc ttcgagtagc tccaggagtt
     2881 tggggaggag ggcgttcgcc tcctcgacgg tgaagagcct ggcgaaggag ggttccacgc
     2941 ccctgggtat gttctcagcg gccgacggcc aggcggtcgg cgagcatgcc ggggaggccg
     3001 gcggaccgga tcttggcctg cgccttccct atgtcgtact cgacgaagcg gtaggtgacg
     3061 cgctcgccct cgacgaggac gtaggaagcg aaggtgtccc cgtcgcgcgg ctgcccgacc
     3121 gagcctgggt tcaccaggta tggtccgttc gagctcaggt cgagagtgtg agcctcgatc
     3181 acgggcccgt tcggcgaggg ggccacggtc ttgacgtgcg tatgcccgaa aaagcagatc
     3241 gggactccag gatattcgct cctcaggatc tccaggttat cctcggcggc ggcggagttg
     3301 atgatgtact cgtccgggtc gcgcaccgag ccgtggacga agagcgcgtc gcgcgtctcg
     3361 atcatgcgcg gacgggtcct caagaaatca gcgttcctct tgctcaggtg ctcgcgggtc
     3421 cacaccacgg ccgcggcagc gacaggattg aaccagttca ggtccgtctc caggtccgtg
     3481 acggcgaggt cgtggttgcc cgagatcacc ggcatcccga gctcgcgcac gatgtcgcag
     3541 cactcgtcgg ggttcgcccc gtagccgatc acgtcgccaa ggcagtagac gatcctgacc
     3601 tcctcgggga tgtcccgcag gacggcctct agcgcctcca ggtttccgtg gatgtcggag
     3661 ataacagcgt acatatctca ggtgcggcgt agtttatacg gtgcgccaat gattgcaaag
     3721 ccctgtttga aggggtagag gcgttgaggt aatatctcga agcggtttcc cgctcgcggg
     3781 cctgtagctc aggggataga gcaacggttt ccggggccgt gtgtcgcagg ttcgaatcct
     3841 gccaggcccg ctccgacgcg aaggctaacg actgcccttg gacccccgcg agtggctatc
     3901 caacaagaat aagtgccaag acagcgtatt ctcgtcttct gaattcgcac gcttcccgtg
     3961 aaataatgct ccagcgtttc cgaacatgag atgggtgtcc tgatgcctga actgacgaga
     4021 gagcagatgc aagaagaaat ccaacgcctc cggccgttta ggcataactt acgattaccc
     4081 tatgggcttc aaacggctcc tccagagtcc caaccgtcac gacagcagca cgacgcaagg
     4141 atagaaacct tgatccgcca cgctttccca gcgttgctca aagagtgcag cggctccttg
     4201 gaagggctga gggtactgga tctcggctgc aacagcggcg gtttctcggt ggaagctcac
     4261 aaaagggggg ctgaatacgt cctcggcgtg gacgttgtgg atcgatactt agagcaagcc
     4321 gcgttcgtca aaacggctct ggacatcggt gatagcgtcg agttcagaaa cgtcggcgtc
     4381 tacgacctgg atcccgacga ggtgggccgc ttcgatgtca cgttcttttt cgggttgctc
     4441 taccacttgg agaatcccgt cttggcgatg aggaaggtta gcgctgttac cgatcacgtc
     4501 ctggttgtgg acacaaatac cattctcacc ggggacaagc aggagacacc gctctggcgc
     4561 atgagcttcc cctcgcccgt gacgtcgcca gacgacccct tggcctcaac gggtttatgg
     4621 agaaatcggg agtactgcga actcaagccg aacgctgctg cggtcacggg tctgctcaaa
     4681 ctcggtgggt tcgaccgggt gcggctgctc gaaccgacgg cggacggtct gccggaggtc
     4741 taccagaatg ggcaccgcaa gacgttcttt gctgtcaagg agaccgcata ggcacctatg
     4801 gtgagg
//
LOCUS       DASTUV010000386         6482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_245264, whole genome shotgun sequence.
ACCESSION   DASTUV010000386 DASTUV010000000
VERSION     DASTUV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245264"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFT03_07800"
     CDS             complement(<1..426)
                     /locus_tag="VFT03_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU4848087.1"
                     /translation="MLFGQLECFLAVARLGNVSRAAEEMFLTQPTLTARLKALEEEVS
                     DQLFVRTSRGMRLTEAGKEFLPYAERSVVSFKEGRRRLEELRGASGGRLVLGASPGVG
                     TYALPGLLERFTAAYPRVSVSVRTGHSEDILEMVLREEVQ"
     gene            553..756
                     /locus_tag="VFT03_07805"
     CDS             553..756
                     /locus_tag="VFT03_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848088.1"
                     /translation="MIPVQYRDPETEEILDRRYEESVPTIGTRVQIGFGHYQVLFRWQ
                     CVPTSCIVYVRRVPREVGARAAA"
     gene            855..2462
                     /gene="aceB"
                     /locus_tag="VFT03_07810"
     CDS             855..2462
                     /gene="aceB"
                     /locus_tag="VFT03_07810"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU4848089.1"
                     /translation="MEKTRTFEEGVEFKASIPEEYAEILTPEAVSFVAKLNREFGGRV
                     EEILEKRKDFQERIDAGEMPDFLPETKDIRDGDWGVAPVPDDLQDRRVEITGPPDRKM
                     LINALNSGASTYMTDFEDANCPTWHNMLESQLNLRDAVRRTISFDDPETGKHYEVGED
                     AAVLIARPRGWHLPEKHMLVDGKQVRGALFDFGLYFFHNAQNLIDQGSGPYFYLPKME
                     HHPEARLWNDVFNFAQDELGIPRGTIKATALLETFPAVFEMDEILYELKDHSAGLNAG
                     RWDYIFSYIKTFRNREGFVLPDRAKVTMTVPFMRAYTQLLIKTCHRRGAHAIGGMAAQ
                     IPIKDDPEANEQAFAKVREDKEREARNGHDGTWVAHPGLVPVAKEVFDEIMPQPNQLD
                     KLREDVDVGAADLYEPYKDDPITEAGLRNNVSVGIQYLGAWLAGRGAVPVFNLMEDAA
                     TAEISRTQVWQWIHHDEGILDDGTEVTTDLFRDILAEELEKIPTFVDPVRTRNDRFDE
                     AAQLFDRISTDDDFVVFLTLPGYEYLD"
     gene            2663..4000
                     /gene="aceA"
                     /locus_tag="VFT03_07815"
     CDS             2663..4000
                     /gene="aceA"
                     /locus_tag="VFT03_07815"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEU4848090.1"
                     /translation="MFNGSGAAAEIAREWESERWEGIERPYSVEDVARLRGSIKIEYT
                     LARMGAERLWELMRTRDYVQSLGALTGNQAVQQVKAGLEAIYVSGWQVAADANLAGEM
                     YPDQSLYPANSVPHVVKRINQALQRADQIHHTEGKNGIYWFAPIMADAEAGFGGALNV
                     FELMKAMIEAGAAGVHFEDQLSSEKKCGHMGGKVLLPTAQGIRNLISARLAADVLGVP
                     TVLMARTDADAANLITSDVDPADAPFLTGERTAEGFYRVRPGLDYAIARSLAYAPYAD
                     VVWCETSEPNIEEAREFAEAIHAEYPGKLLGYNCSPSFNWKLKLSDEDIATFQEQLGA
                     MGYKFQFITLAGFHSLNYSMFELARDYRARGMAAYSELQQAEFAAEKYGYTATKHQRE
                     VGAGYFDEVAQVVAGGTASTTALTGSTEEEQFDAPQSPITGLPGSTEDEQFVR"
     gene            4056..4244
                     /locus_tag="VFT03_07820"
     CDS             4056..4244
                     /locus_tag="VFT03_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848091.1"
                     /translation="MKESDVRVAGEEQGRLLPDVPPASGQAGVLDGSGPAKREADDEW
                     LEIATGDPAFFIKPSDTV"
     gene            complement(4345..4611)
                     /locus_tag="VFT03_07825"
     CDS             complement(4345..4611)
                     /locus_tag="VFT03_07825"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEU4848092.1"
                     /translation="MDEHKLDGNAAGGPLGEAFAFEVTTAEGACGNCGTVAHMGEAMA
                     YMTEIGTVVRCPTCDNALIRFVHNPRGCFLDARGVSYLHFESAP"
     gene            complement(4614..5369)
                     /locus_tag="VFT03_07830"
     CDS             complement(4614..5369)
                     /locus_tag="VFT03_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEU4848093.1"
                     /translation="MVETRHLPRRLVWRIGEVVETHHATPRTKSLFLDVPGWEGHKAG
                     QHVDVRLTAEDGYQAQRSYSIASAPEEENVELVVERLDDGEVSPYLTDVLRTGDKLEL
                     RGPIGGWFTWEVREGGPLFLVAGGSGIAPLAAMIRHRAAAGSEAPCRLLYSSRSREDT
                     IFADELERLAASDGSLAVIETLTRSQPPGWTGYARRIDDEMLAEVAPSPEQGPLTFVC
                     GPTPLVESVATSLVGLGHDAGRIKTERFGPTGG"
     gene            complement(5371..5970)
                     /locus_tag="VFT03_07835"
     CDS             complement(5371..5970)
                     /locus_tag="VFT03_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEU4848094.1"
                     /translation="MSFFSRGFRGRRRQTDERLPPGQYLVEGFPVLSAGPTPRTPLEE
                     WDFSVVGEVDEPKRWTWEEFRALPSEEVTRDIHCVTKWSKLGTHWEGVSVDTLLEGVE
                     TAAGYVLAFCDGGYTTNLPLEDVTGGKAWIAYSFDGEPLEPEHGGPARLLVPHLYFWK
                     SAKWVRGLRLLSHDEPGFWESLGYHNYGDPWREQRYWGD"
     gene            6270..>6482
                     /locus_tag="VFT03_07840"
     CDS             6270..>6482
                     /locus_tag="VFT03_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848095.1"
                     /translation="MRKKLLLLVGVVAITLISASSSFAQDPGQTPPATASPSAQSPPP
                     EQSAPPEQSAPPEQSAPPEQSAPPEQS"
BASE COUNT         1237 a         2071 c         2072 g         1102 t
ORIGIN      
        1 ctgtacctct tccctcaaca ccatctccag gatgtcctcc gagtgcccgg tccgcaccga
       61 gaccgagacc cgcggatacg ccgccgtgaa cctctccagt agcccgggca acgcataggt
      121 cccgaccccc ggcgacgctc cgagtaccag ccgcccgccc gaagcccccc gtagttcctc
      181 cagtcgccgc ctgccctcct tgaagctcac caccgagcgc tcggcgtaag gcaggaactc
      241 cttcccggcc tccgtgagcc gcatcccgcg gctcgtcctc acgaagagct ggtcgctaac
      301 ctcctcctcc aaagccttca gccgcgccgt cagcgtcggt tgcgttagaa acatctcctc
      361 cgcggccctg ctcacgttcc cgagccgcgc caccgccagg aaacactcca gctgtccaaa
      421 cagcaccgca gcctccaaac gttctgatat aggttttctc tgtggaaata atataagcaa
      481 acaattggac caatggcaag ggtgagagta tcgtggtcgc acgcgatagg aagtaagaga
      541 caggagggtt ccatgatccc cgttcagtac agagacccgg agaccgagga gatcctggac
      601 cgccgctacg aggaatccgt cccaaccata gggacgcggg tgcagatcgg tttcgggcat
      661 taccaggtgc tcttccgctg gcagtgtgtg ccgaccagtt gcatagtcta cgtgcggcgg
      721 gtgccgcggg aagttggcgc tcgcgccgcc gcctagcagg gggcgttttc ggggctccga
      781 gcgttgggta cgacagcttg ctagagtatt cgctctagct ggccagtcga agaaacggag
      841 aggagctcca aataatggag aagacgcgca cctttgaaga gggggtcgag ttcaaggcct
      901 ccatccctga ggagtacgcc gagatcctta cgcccgaggc ggtctccttc gttgcgaagt
      961 tgaaccggga gttcggcggg cgggtagagg agatcctgga gaagaggaag gacttccagg
     1021 agcggatcga cgccggcgag atgccggact tcctacccga gacgaaggac attcgggatg
     1081 gcgactgggg agttgcgccc gtgccggacg acctccagga caggcgggtg gagatcacgg
     1141 gcccgccgga ccggaagatg ctgatcaatg ccctcaactc gggcgcgagc acgtacatga
     1201 cggacttcga ggacgcgaac tgcccgacgt ggcacaacat gctcgagagc cagctcaacc
     1261 tccgcgacgc ggtgaggcgc acgatctctt tcgacgaccc cgagacgggc aagcactacg
     1321 aggtgggcga ggatgctgcg gtcttgatcg cccgcccccg cggttggcat ctccccgaga
     1381 agcacatgct ggtcgacggc aagcaggtcc ggggcgccct cttcgacttc ggcctttact
     1441 tcttccacaa cgctcagaac ctcatcgacc agggttcggg accgtacttc tacctgccca
     1501 agatggagca tcatcccgag gcgaggctct ggaacgacgt cttcaacttc gcccaggacg
     1561 agctaggtat accgcggggc accatcaagg cgacggcgct cctcgagacg ttcccggcgg
     1621 tcttcgagat ggacgagatc ctatacgagc tcaaagacca ctctgcgggc ctcaacgccg
     1681 ggcgttggga ctacatcttt agctacatca agacgttccg caacagggag ggcttcgtcc
     1741 tccccgacag ggcgaaagtc accatgaccg tcccgttcat gcgggcatac acgcagctcc
     1801 tgatcaagac ctgccatcgc cggggcgccc acgccatcgg cgggatggcg gcccagatcc
     1861 ccatcaagga cgatcccgag gcgaacgaac aggccttcgc gaaggtccgc gaggacaagg
     1921 agcgcgaggc gagaaacggc cacgatggca cctgggttgc gcaccctggc ctcgtccccg
     1981 tcgccaaaga ggtcttcgac gagataatgc ctcagcccaa ccagctcgac aagctgcgcg
     2041 aagacgtcga cgtcggcgcc gcagacctct acgagccata caaagacgac ccgatcaccg
     2101 aagccggcct ccgcaacaac gtaagcgtcg gcatccagta cctcggagcc tggctggcag
     2161 gccgcggggc cgtccccgtc ttcaacctca tggaagacgc cgcgaccgcc gagatctctc
     2221 gcacccaggt gtggcagtgg atccaccacg acgagggtat cctcgacgac ggtacagagg
     2281 tgaccacaga cctcttccgc gacatcctcg ccgaggagct cgagaagatc cccaccttcg
     2341 tcgaccctgt gcgcaccagg aacgaccgct tcgatgaggc cgcccagctc ttcgaccgga
     2401 tctcgacaga cgacgacttc gtcgtgttcc tgaccctgcc cggctacgag tacctggatt
     2461 aggagcgaga agatacccgc ctatcactac atcccatccg gcggcacagc ttacaggccg
     2521 ccggtccgcg cctcccgcac aggaccgccc gcgcactatt tgacacgttc cgagaaggtg
     2581 tacacaatgg tgctatgcaa actacacgta tgtttgccga acacttaagt gacgagcgaa
     2641 cgacggtagg agaggagagg cgatgttcaa cgggagcggt gcggctgcgg agatagcccg
     2701 ggagtgggag tccgagcgct gggagggcat cgagcgtccg tactcggttg aggacgtggc
     2761 gcgattgcgg gggtccatca agatcgagta cacgctcgcg cggatgggtg cggagcggtt
     2821 gtgggagttg atgcgcaccc gcgactacgt ccagtctctg ggagctctga cgggcaacca
     2881 ggctgtgcag caggtcaagg ctggcctgga ggcgatatat gtctccggct ggcaggttgc
     2941 tgccgacgcc aacctggctg gtgagatgta tcctgaccag agcctgtacc cggccaacag
     3001 cgtgccgcac gtcgtcaaga ggatcaacca ggccttgcag cgggctgacc agatccacca
     3061 taccgagggg aagaacggga tctactggtt cgcgcccata atggccgacg ccgaggctgg
     3121 tttcgggggt gcgctcaacg tcttcgagct gatgaaggcc atgatcgagg ctggtgccgc
     3181 gggtgtccac ttcgaggacc agctctcctc agagaagaag tgcgggcaca tgggtggcaa
     3241 ggttctgttg ccgacggccc aggggatcag gaacctgatc tctgcgcggc tggctgcgga
     3301 cgtgctcggg gtaccgacgg tcctcatggc gcgtacggac gccgacgcgg cgaacctgat
     3361 cacctccgac gtggacccgg cggacgctcc gttcctcact ggcgagcgga cggcggaggg
     3421 cttctacagg gtgaggcctg gcctggacta cgcgatagca cgcagcctcg cctatgcgcc
     3481 gtacgcggac gttgtgtggt gcgagacctc cgagccgaac atcgaggaag ccagggagtt
     3541 cgccgaagcc atccacgccg agtaccctgg caagctcctc ggctacaact gctcgccctc
     3601 gttcaactgg aagctcaagc tctcagacga ggacatcgcc accttccagg agcagctcgg
     3661 cgcgatgggc tacaagttcc agttcatcac cctggccggc ttccactccc tcaactacag
     3721 catgttcgag ctggcgcgcg actatcgcgc gaggggcatg gccgcgtact ccgagctgca
     3781 gcaggccgag ttcgcggccg agaagtacgg ctacaccgca accaagcacc agcgcgaggt
     3841 cggcgccggc tacttcgacg aggtggccca ggtcgtcgct ggcggcaccg cgtccaccac
     3901 agccctcacc ggctccaccg aggaggagca gttcgacgcg ccgcagagcc cgatcacggg
     3961 cctccccggc tcgaccgagg acgaacagtt cgtcaggtag gcttcaggag ctggaggtac
     4021 agccctcttc tcgtgggggc cgaaaggagt ggtgaatgaa ggagagcgac gtacgcgtgg
     4081 ccggtgaaga gcaggggcgt ctcctgccag acgtgccgcc cgcttcgggg caagctggcg
     4141 tcctggacgg ctccgggccg gcgaagcgtg aggctgacga cgagtggctg gagatcgcaa
     4201 ccggggaccc ggcgttcttc atcaagccct ccgacactgt gtaggtagct cttttgcggg
     4261 tgatagggaa gaggggccgg gtaaccggtc cctctttcgt tgtctgaggc tactcgtcgg
     4321 atctcggata gataagagct ggcttcaggg agcactctcg aagtgcaggt agctgacgcc
     4381 ccgcgcgtcg aggaagcaac ccctcgggtt atgcacgaaa cgtatgagcg cgttgtcgca
     4441 ggtggggcag cgtacgaccg tgccgatctc ggtcatgtaa gccatcgcct cgcccatgtg
     4501 cgcgaccgtg ccgcaattgc cgcaggcgcc ctccgcggtg gtcacctcga aggcgaaggc
     4561 ctccccgagc ggcccgccgg cggcgttccc gtccagcttg tgttcgtcca tattcagcct
     4621 ccagtcggtc cgaagcgttc ggtctttatg cgtcctgcgt cgtgcccgag ccccacgagc
     4681 gacgtagcaa ccgactcgac tagcggcgtc gggccgcaga cgaaggttag cgggccctgc
     4741 tcgggagacg gcgcgacctc cgcgagcatc tcatcgtcga tgcggcgggc gtaacccgtc
     4801 cagccgggag gctgggagcg ggtgagggtt tcgatcaccg cgagggagcc gtccgaggcg
     4861 gcgagccgtt cgagctcgtc tgcgaagata gtatcttcac gcgagcgtga ggagtagagg
     4921 aggcggcagg gagcttcgct tccggcggcg gcgcggtgcc ggatcatggc cgcgagcggc
     4981 gcgatgcccg agcctccggc cacgaggaac agggggccgc cctctctcac ttcccacgtg
     5041 aaccagcccc cgatcggacc gcgtagctcc agcttgtctc cggtgcgcag cacatctgtc
     5101 aggtaagggg agacctcgcc gtcgtcgaga cgctcgacca cgagctcgac gttctcttcc
     5161 tctggcgcgg atgcgatcga gtagctgcgt tgggcctggt agccatcctc ggcggtgagg
     5221 cgaacgtcca cgtgctggcc cgctttgtgc ccttcccagc ccggaacgtc gaggaacagg
     5281 ctcttggtgc gcggcgtggc gtggtgggtc tcgacgacct cgccgatacg ccagaccagc
     5341 ctacggggaa ggtgccttgt ctcgaccatc tcagtccccc cagtagcgct gctcgcgcca
     5401 ggggtcgccg tagttatggt agccgagcga ctcccagaag cccggttcgt cgtggctcaa
     5461 gagcctgagc cccctgaccc acttggcact cttccagaag tagaggtggg ggacgaggag
     5521 gcgcgcaggt ccgccgtgtt ccggttcgag aggctcgccg tcgaaggagt aggcgatcca
     5581 ggccttgcca cccgtgacgt cctcgagcgg caggttcgtg gtgtagcctc cgtcgcagaa
     5641 cgcgagcacg tacccggccg cggtctcgac cccttcgagc agggtgtcca cggatactcc
     5701 ttcccagtgg gtgccgagct tcgaccactt ggtgacacag tggatgtcgc gggtgacttc
     5761 ctccgatggg agcgccctga actcctccca cgtccacctc ttcggctcgt caacctcgcc
     5821 gacgaccgag aagtcccact cctcgagcgg tgtgcggggc gtggggcctg cggagaggac
     5881 agggaagccc tcgaccaggt actggcccgg cggcaagcgt tcgtccgtct gcctgcggcg
     5941 gccccgaaag ccgcgtgaaa agaagctcac gactcaaccc cctgacgtcg ggttagtgta
     6001 ggaagataaa ttctgccccc ggacgctcgc gcggtcaaac gtttttggga gttttggggc
     6061 tagtgatgga acggaatttg taagaacagg gcgtttttca gggaagctcg gtgtttagag
     6121 ttgaattctt cgtaggacat ttgtcccgac ttactcctat ctagtccatt tgagggatgc
     6181 gctgcctctg aacctaatac tacactggct tggcacgaga cttgtgggag aacagagagc
     6241 cctgtcaaat ggggaaaggg gagttgctaa tgaggaagaa gttgctcctg ttagttgggg
     6301 tagtggcgat aaccttgatc agcgcatctt cctccttcgc ccaggacccc ggccagactc
     6361 cgccagcaac ggccagccct tctgcacaga gccctccacc cgaacagagc gctccacccg
     6421 aacagagcgc tccgcccgag cagagcgctc cgcccgaaca gagcgctccg cccgagcaga
     6481 gc
//
LOCUS       DASTUV010000387         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1077123, whole genome shotgun sequence.
ACCESSION   DASTUV010000387 DASTUV010000000
VERSION     DASTUV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077123"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..673)
                     /locus_tag="VFT03_07845"
     CDS             complement(287..673)
                     /locus_tag="VFT03_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848096.1"
                     /translation="MRSDASEHVTLVEVLELTSTLEEFTAAIQAAARRLEAEGIQELV
                     TLQFYATPGSAEVGALLTFTDRSRIMEHIDMITEWEEFERFFSTTKPLDVRVYGKLSA
                     EAEAWVRQFGDIVSKKFEYHVAGFVR"
     gene            877..1530
                     /locus_tag="VFT03_07850"
     CDS             877..1530
                     /locus_tag="VFT03_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848097.1"
                     /translation="MAIRVLITDDHGVVRQGLRMFLSLDPEIQVVGEAQDGREALAMA
                     RELKPDVVLMDLLMPVMDGLESTKAIRTELPEVEVIALTSVLEGASVTGAIRAGAIGY
                     LLKDTDVEGLHQAIRGAAEGGVHLSPEAAAHLMREVRAPENPEALSDRETEVLRLLAR
                     GKANKRIASELYIEEHTVKAHVSSILMKLGVQSRTQAALHAVRTGLVSVEELGDESW"
     gene            complement(1666..3450)
                     /locus_tag="VFT03_07855"
     CDS             complement(1666..3450)
                     /locus_tag="VFT03_07855"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4848098.1"
                     /translation="MSDDGHDEKRPAHAASDVPVRDVSVDVHARKPVIFAVDAGVASF
                     GRIEYGLRRRYGVEYQVICETSAMWGMKKLRDLRAAGEEVALILADQWMPDITGDEFL
                     ARASQLFPTAKRAVLVEWGDRRIQEPVLRAMAVGDIDYYVNKPERPGDEYFHRMIAEF
                     IYDWAKTHRPVFAEIRVVGEQWSARSHELRDILNRNGILHEFHAADTEVGREILARIG
                     KSSARFPVVVLYDGQVLEDPSNAELVDAFGINRELDRRSFDLVIVGAGPAGLAAAVYG
                     ASEGLGTLVLEGEAIGGQAGTSSLIRNYLGFPWGVAGAELAKRATEQAWWFGAVFRFM
                     RNAAALRSERRGLTLTLSDGAEVTSRAVVLATGVAYRRLGVPSLEEHVGMGVFYGAAV
                     SEAQAMKGEEVFVVGGANSAGQAAVHLSEYASRVTLLVRARSLETSMSDYLIKEIEAA
                     QNIQVRFGTQVVDGGGEGRLDHLVLKDLASGLTEAVPAAALFVLIGAEPRTGWLPDEI
                     VRDERGYIVTGPDLLQDGQPPRGWPLGRPPLPMETSMPGVFAAGDVRYGSVKRVASAV
                     GAGAIAIQSVHEHFVKARLGLDSSGNIG"
     gene            complement(3694..>4455)
                     /locus_tag="VFT03_07860"
     CDS             complement(3694..>4455)
                     /locus_tag="VFT03_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU4848099.1"
                     /translation="PLHEFLPDSKDLAKEIAEREGRGEDATELSRQLRVAENLEERNP
                     MLGLRGCRLGLLRPDVYLMQARAIAQAAREVREAGGEPVVEIMIPLVGFSSELRKMRD
                     LIEVELAGESLPIGTMIELPRACTVAGEIAHVADFFSFGTNDLTQMVCGISRDDAEEK
                     FLSQYLESGLLQDNPFETIDQDGVGELVQMATERGREANPRLKLGVCGEHGGDPESVS
                     FFHEVGLDYVSCSPFRVPGARLAAAQAAVSQPGRT"
BASE COUNT          829 a         1453 c         1333 g          840 t
ORIGIN      
        1 aagctcgtca acgccgtgca acgcgacggt cgagtcttcc tctcctcgac gatagtcgac
       61 ggaaagttca ccctgcgggc ggccatcttg agccttcgca cccacctgga gacgatcgag
      121 ctggccatcg aagtactgcg cgaaaaagcg aagcagctcg agcagggtgg ctaacctgaa
      181 gagctcgaca accggcttga tcgacgaact ctcaccccga agggtctcga acgagcacaa
      241 ggcgagccaa ttctccgcaa cgtggtcatt ccggtggccg tgacggttag cgcacgaagc
      301 cggccacgtg atactcgaac ttcttgctca ctatgtcgcc gaactgccgc acccacgctt
      361 ctgcctccgc gctcagctta ccgtacaccc gcacgtcgag cggcttcgtc gtactgaaga
      421 acctttcgaa ctcttcccat tctgtgatca tgtcgatgtg ctccataatt cggctgcgat
      481 cagtgaaagt tagaagtgcg cccacctcgg cagagccggg agtagcgtag aactgcaagg
      541 tcaccagctc ctgtatccct tcggcttcga gccgtcgcgc tgccgcctgg atggcagcgg
      601 tgaattcctc gagagtactg gtcagctcga gcacctctac gagggtgacg tgttcgctgg
      661 catctgatct cacgccggtc tctccttttg ctcgtgcggg taatttctgc tctactctat
      721 tctgccttct tccagaggac caggctctag agaagcccgc tctgccgcag ctttgcttgc
      781 ctctaaacgg cccacggcga caactgtcga aggtacgaat gttgtcgtgg acgttcgtca
      841 gaacgataat gtaggaccaa agtcgtagga gaaaccgtgg cgataagagt tctgataact
      901 gacgaccatg gcgtggtgcg ccaggggctg cgcatgttcc tctctctcga ccctgaaatc
      961 caggtcgtgg gagaagccca agacggccgg gaagccttgg cgatggcccg ggagctcaaa
     1021 cccgatgtgg tgcttatgga cctgctgatg cccgtcatgg acggtctgga atctaccaag
     1081 gccattcgaa cggaactgcc ggaagttgag gtcatagcct tgacaagcgt gctggagggg
     1141 gcctcggtga cgggggctat cagggcaggg gcgatcggct acctcctcaa ggacactgac
     1201 gtcgaggggc tgcaccaggc aataaggggc gcggccgagg gaggagtgca cctttccccc
     1261 gaggctgcgg cgcacctgat gcgcgaggtg cgcgcccccg agaacccaga ggcgctaagc
     1321 gaccgcgaga ccgaggtctt gaggctcctc gcccgcggca aggccaacaa acggatagcc
     1381 agcgagctgt acatagaaga gcacacggtc aaggcccacg tctcgagcat cctcatgaag
     1441 ctcggcgtcc aaagccgcac gcaggctgcc cttcatgccg tacgcactgg gttggttagc
     1501 gtcgaggaac tcggcgacga gtcgtggtga agcactcgcc gccaagcaag cctggccgcg
     1561 gcctgcagtc gctcagggta attcctcgtg ttcacgtcgc tcagctgcat cttcgatcct
     1621 tgagttgacc ggtcccgaag aggtcacggc agcatgggct gacgttcatc cgatatttcc
     1681 cgagctatcc agaccaagcc tggctttgac aaagtgctcg tggaccgatt ggatggcgat
     1741 cgcgcccgcc ccaacggcgg aggcaaccct cttgaccgag ccgtacctga cgtcgccggc
     1801 agcgaagacg cccggcatgc tggtttccat tgggagagga gggcgcccga gcggccagcc
     1861 gcggggaggt tggccgtctt gtaagagatc cgggccggtg actatgtagc ctcgctcgtc
     1921 gcgcacgatc tcgtcgggca gccaccccgt ccggggctct gcaccgatga ggacgaacag
     1981 agctgcggcc gggaccgcct cggtgaggcc cgaggccaga tccttcagga ccaggtggtc
     2041 caacctccct tctccaccgc catcgacgac ctgagtgccg aaacgaacct ggatgttctg
     2101 cgccgcctcg atctccttta tgaggtagtc ggacatgctc gtctcgaggg agcgagcgcg
     2161 cacaagtagc gtcacgcgcg aggcatactc cgagaggtgt acggctgctt gaccggccga
     2221 gttggccccg ccaactacga agacttcctc gcccttcatc gcttgggcct cgctcaccgc
     2281 ggccccgtaa aacaccccca taccgacgtg ttcctccagg ctcggcacgc ccaggcgtct
     2341 gtaagccacg ccggtggcca gaaccacagc tctgctggtc acctcagccc cgtcggaaag
     2401 cgttagcgtc agtcctctcc tctcggatcg cagtgcggca gcgttccgca tgaagcggaa
     2461 gacggcgcca aaccaccacg cctgctcggt ggccctcttg gcgagttcgg cgcctgctac
     2521 accccaaggg aacccgaggt agttgcggat cagcgagctc gtccctgcct ggccgcctat
     2581 ggcttctccc tccaggacga gggtgcctag gccctccgag gcgccgtata cggccgccgc
     2641 cagcccggcc ggtcccgccc ccacgatcac caaatcgaag cttcgccgat ccagctcacg
     2701 gttgatgccg aatgcgtcaa cgagctcggc gttcgaggga tcttcgagca cttgcccgtc
     2761 gtaaaggacg accacgggga acctggcgga actcttgcca atccgggcga ggatctcccg
     2821 gcccacctct gtgtccgcgg cgtgaaattc atgcaagata ccgttgcggt tgagaatgtc
     2881 cctcagctcg tgcgagcgcg cagaccactg ttcgcccacc acgcggatct cagcgaacac
     2941 gggacggtgg gttttggccc agtcgtagat gaattccgct atcatcctgt ggaagtactc
     3001 gtcgcctggg cgctcgggct tgttgacata gtagtcgatg tcgcccacgg ccatcgctcg
     3061 gaggaccggt tcctggattc tcctgtcgcc ccactcgacg agcaccgccc gcttggcggt
     3121 tgggaaaagc tgactcgcgc gggcgaggaa ctcgtcgccg gtgatgtcgg gcatccattg
     3181 atcggcaagg atgagagcta cctcctcacc ggccgccctg aggtccctca acttcttcat
     3241 accccacatt gccgacgtct cgcaaattac ctggtactcg acgccgtagc gcctgcgaag
     3301 tccgtactcg attctgccga atgatgcgac ccctgcgtcg acggcaaaga tcacgggttt
     3361 gcgcgcgtgc acatctacgg agacgtcccg aacgggaaca tcgcttgctg cgtgggctgg
     3421 cctcttctca tcatggccat cgtccgacat ccaggctcac ctccctgagc tcctttctcg
     3481 cgtccgagat aatatcgtgc tcacgttggg cagcgtagcg cgcggtagcg gtgcattggc
     3541 aggaggactg aaccatcctt aacgtccgaa ctcctctgag agggcgcaat ccgcgcaaat
     3601 acacgccccg ctagacctcg acaccagctt agggcatcgg cgagggtagc atatccgcca
     3661 aattgactat ttgtggacgt tgtgggtcga ggactaagtt cgtccaggct ggctcacagc
     3721 ggcttgtgcg gcagccaggc gcgcgcctgg cacccggaag ggcgagcagg agacgtagtc
     3781 gaggcccact tcgtggaaga aggagacgct ctccgggtcg ccgccgtgct ccccgcagac
     3841 gccgagcttc aggcgcgggt tcgcctcccg tccccgctcg gtggccatct ggaccagctc
     3901 acctacgccg tcctggtcga tggtctcgaa ggggttgtcc tgcagcaggc cgctctcgag
     3961 gtattgggag agaaacttct cctcggcgtc gtcgcggcta atgccgcaga ccatctgagt
     4021 gagatcgttg gtgccgaagg agaagaagtc ggcgacgtgc gctatctccc cggcgaccgt
     4081 acacgcccgc ggcagctcaa tcatcgtccc gatgggcaag ctctctccgg cgagctcaac
     4141 ctcgatcaga tcccgcatct tcctcaactc cgaggagaag ccgacgaggg gaatcattat
     4201 ttcgacgacc ggctcgcccc cggcttccct gacttccctc gcggcctgcg caatggcacg
     4261 agcctgcatc aggtagacgt cgggtcgcaa gagcccaagc ctgcagcccc gcagccccag
     4321 catcgggttc ctctcttcga ggttctccgc tacgcgcagc tgtcgactga gctctgtggc
     4381 gtcctccccc cgcccctcgc gctcggctat ctccttcgcc aggtccttcg agtcagggag
     4441 gaactcgtgt agcgg
//
LOCUS       DASTUV010000388         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_47378, whole genome shotgun sequence.
ACCESSION   DASTUV010000388 DASTUV010000000
VERSION     DASTUV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47378"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFT03_07865"
     CDS             complement(<1..252)
                     /locus_tag="VFT03_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848100.1"
                     /translation="MFGKRGVIQTDATKRNSQTPFRDPGAMTGLLTGVSFIGGVGGAM
                     ALADSPYPRPGSEPAEIRRYFTENSRSARISATGQLISTV"
     gene            complement(523..>2276)
                     /locus_tag="VFT03_07870"
     CDS             complement(523..>2276)
                     /locus_tag="VFT03_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4848101.1"
                     /translation="VVLAFCMGMVLTFAVVLVSSWRVSRLNVVRAIRDIPEPDKKGRS
                     VLGVLVAVLTPIAGAVALWQGLETRTTAFYLGGLSLILIGAALVARVLGISDRVAFSA
                     SGIILLALWLTPASITTPADMARGPEMFFVSGIALVVAGVWLVIFNADVILRVVVALF
                     GRIKGLTPVLKTAVKYPTQSLFRTGMTLAMFMLVVFTLTAMNFIQAAMGAAFGDTQEL
                     SGGYEIRADAGYADPIPDMNAALEGAKGLKSDIEAVGQVSNLPAEVKQKGTDREPGSI
                     YVQGVDEGYSKSVSYDFQSTARGYGSDRDVWDALQTEQDVAVISSNLAPQRNASTFGS
                     SVEPPVKLTGFYADDESLPDDLYLRVEDPESGRTRELRVIGVLESSASFAGQIVTSQK
                     TLEGLAGRSVPPQSYYFDLADGTNAAATARTLEKDFAKNGLQTELTAEVIRDSDATRR
                     IVFLLLRGFMGLGLVVGICALGVITARSVVERRQQIGMMRALGFQRGQVRFAFLIESS
                     FVALLGLGFGVALGLAFSGTLIDNIREGFPGMEYRVPWSALVLVIVVGYVASLVTTYL
                     PARQASKVYPAEALRYE"
BASE COUNT          434 a          740 c          715 g          387 t
ORIGIN      
        1 gacagtcgag atcagctgac cggtagcgct gatccgagcc gagcgggaat tctcggtgaa
       61 gtagcgccgg atctccgcgg gctccgagcc gggacgcggg tatggggagt cggcaagggc
      121 catcgctccg ccaacgccgc cgataaagct gacgccggtc agcagaccgg tcattgctcc
      181 cggatcacga aacggggtct gtgagtttct cttggtggcg tctgtctgga tgacgcctcg
      241 tttgccgaac atgtgtttcc tccttgtgat cggtcgtcgg gtcgatctat tcaaattgcg
      301 ttacgtacac gaggaaccac cacggttacg gcgacgccct ggcaccctgc gccgcggctc
      361 gccccttgcc atttcccggc atcccgcctc cggtgatggg tagagcgagg cccccgagcg
      421 atccctcgct catcttcgac ccagcctatt gaggacttcc tcagaaccag gccgagcacc
      481 ggtgcgaccg ccagggccgt cgcacgcttc cggagatcag gtctactcat aacgcaacgc
      541 ctctgccggg tataccttcg aagcttggcg ggcgggcagg tacgtggtca cgagagatgc
      601 cacatatccg acgacgatca caagcacgag cgccgaccat ggcactctgt actccatccc
      661 cggaaagccc tcccgaatgt tgtctatgag cgtgccggag aaggcgagcc ccagggcgac
      721 gccaaacccg aggcccagca acgcgacgaa ggaggactcg atcaggaagg cgaaccgaac
      781 ctgccccctc tggaaaccca gggcccgcat catcccgatc tgctggcgcc tctccaccac
      841 agagcgggcc gtgatcacgc ccagagcgca gatgccgacc accagcccca ggcccatgaa
      901 accccgcaag agcaaaaaga cgatcctgcg cgtcgcgtcg ctgtcccgga tgacctcggc
      961 agtcagctct gtctggaggc cgttcttggc gaagtccttc tccagggttc tggccgtagc
     1021 tgcggcgttc gtgccatcag cgaggtcgaa gtagtagctc tggggcggaa cggaacggcc
     1081 ggccagccct tcgagcgtct tctgcgaggt cacgatctgt ccggcgaagg aagccgagct
     1141 ttcgagcacc cctatcacgc gcagttcccg cgtcctgccg gactcggggt cttcgactcg
     1201 caggtagagg tcatcgggca ggctctcatc gtcggcgtag aagccggtga gcttgacggg
     1261 aggctccacc gaggaaccga aggtcgaggc gttacgctgg ggggcgaggt ttgaggagat
     1321 cacggcgacg tcctgctctg tctggagtgc gtcccacacg tcccggtcgg agccgtagcc
     1381 cctggcggta gactgaaaat catagctgac gctcttcgag tagccttcat ccacgccttg
     1441 cacgtagatc gatcctggtt cccggtccgt gcccttctgc ttgacctccg ccggaaggtt
     1501 ggagacctgg cctacggcct cgatgtcgct cttcaacccc tttgcgcctt cgagtgcggc
     1561 gttcatgtcc gggatggggt cggcgtagcc tgcgtcggcc cgtatctcat agccgccgga
     1621 gagctcctgc gtgtccccga aggcggcacc catcgcggcc tggatgaagt tcatcgccgt
     1681 caaggtgaag acgaccagca tgaacatcgc cagcgtcatg ccggtccgaa agaggctctg
     1741 ggtaggatac ttcaccgccg tcttgaggac cggggtaaga ccctttatac gcccgaagag
     1801 ggctaccact acccgcagga tcacgtcggc gttgaagatc acgagccaca ccccggcgac
     1861 cacgagagct attccggaga cgaagaacat ctccggcccc cgcgccatat ccgccggtgt
     1921 ggtgatggag gccggggtca gccacagcgc gagcaggatt attccagatg cgctgaaggc
     1981 cacgcggtca gatattccga ggacacgagc caccagcgcc gcgccgatca agatcagcga
     2041 gagaccgccg aggtagaagg ccgtcgtccg ggtctcgagc ccctgccaga gagccactgc
     2101 gcccgcgatg ggcgtcagga cggccactag aacgcccagg acgctgcgcc ccttcttatc
     2161 cggctcgggg atgtcgcgga tggcgcgcac gacgttgagg cggctcacgc gccaggagga
     2221 gacgaggacg accgcgaagg tgaggaccat ccccatgcag aaagcgagaa ctacgt
//
LOCUS       DASTUV010000389         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_234572, whole genome shotgun sequence.
ACCESSION   DASTUV010000389 DASTUV010000000
VERSION     DASTUV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234572"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFT03_07875"
     CDS             complement(<1..531)
                     /locus_tag="VFT03_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848102.1"
                     /translation="MSETTYPGGPPAGTSTGVPRLLGSPLEVGGVVLRNRIVSPPIER
                     NYCALDGSVTERYIAYLRARAAGGAALLFTEATYVRADGRGRLREMGAHDDHVIPGLR
                     VLAGAVHAEGALLGVELVHAGRVAQTFVSGFQPVAPSPVPCEISGGEMPRELATQEIG
                     ESVRSFADAARPCVEAG"
     gene            complement(969..1760)
                     /locus_tag="VFT03_07880"
     CDS             complement(969..1760)
                     /locus_tag="VFT03_07880"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4848103.1"
                     /translation="MTEGREGDGPVRGLVAGGAGGIGRHIVAQMVEAGYEVTIADLDR
                     EGAEQVRADTGAARTWVGDLTAEKGVREAVDAARDGGVLHALVIATGISPKKDGRKRP
                     FYEISLEEWSQVMAVNLTGPFLLCREAYPHLARDGRASIVTILSIMAKLGASGPEGAF
                     PPYSPSGAHYAASKAALHNLTVSVSRELAPEGIRCNGVAPGYVGAGMVGTTDAELAQR
                     MRSQVPLGRPASPEEIADVVGYLLSDRSTYITGETIDVDGGWHPD"
     gene            complement(2021..2896)
                     /locus_tag="VFT03_07885"
     CDS             complement(2021..2896)
                     /locus_tag="VFT03_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU4848104.1"
                     /translation="MNASPQVGIDHLTLLDISPPELVTIAGEVGFDSVGLRVAAATPG
                     EEPWPMAVGSSMLEETIRRSDDTGVRALAVEVVRLGPETRREDYEFALQAGARLGASY
                     VMVNGDDPDIERACDTFATLTADAKPYGLRPLIEPIIYTQVSNLDEAVYIAERSDGGG
                     ILLDSLHFQRYDGSFEHLRSVNPGLLAYAQLNDAPLRPPTNLPRPSRLPRGQSTDGSD
                     LQLESRAMRLLPGDGELPLAQFLVALPEEIPLSVEAPALPLRETLTPVEFARRARQAM
                     SDVIAAARRYADQQR"
     gene            complement(2966..>3845)
                     /locus_tag="VFT03_07890"
     CDS             complement(2966..>3845)
                     /locus_tag="VFT03_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU4848105.1"
                     /translation="GFEWVQPPVTLGHEFSGIVEEVGQGVSGVSPGDRVVAVAVQGCG
                     RCEVCHAGSPQLCPWREAIGLSRDGGMAEYAVISEEHLIPVPEGLDLAVAALGEPLAV
                     AVHAVNVRADIEPGQQVVVSGPGPIGILCAMLAQLRGAEVLLTGVGKDSESRLPAAER
                     VGLRTANLSDKSLEEHLQDSFGDQGPIVWVESSGSVQALGSALESVRPGGAVIVVGLY
                     AEEMRFSPTLAVRRELSLLFSYSCNQADYRVALDLLGQGTIDPTPLLSKYALEDAPRA
                     FEVVGQGEAVKAILVP"
BASE COUNT          672 a         1301 c         1164 g          708 t
ORIGIN      
        1 ccccgcctcg acgcaggggc gggcggcgtc ggcgaacgac cgcacgctct cgccgatctc
       61 ctgcgtagcg agctcccgcg gcatctcgcc gccggagatc tcgcagggca ccggcgatgg
      121 cgcgacaggc tggaagcccg agacgaacgt ctgcgcgacg cgcccggcgt ggaccagttc
      181 cacgccgagc agggcgccct cggcgtgcac cgcgccggcg aggactcgga ggcctgggat
      241 cacgtggtcg tcgtgggcac ccatctcgcg caggcgtccg cgcccgtctg cacggacata
      301 ggtggcctcg gtgaacagca gcgccgcgcc ccccgccgcg cgggctcgca gataggcgat
      361 gtagcgctcg gtgaccgaac catccagggc gcagtagttc cgctcgatcg gcgggctgac
      421 gatccggttc cgcagcacga ccccacccac ctcaagggga gagccgagca ggcgcggcac
      481 gccggtggac gtgccggccg ggggaccgcc tggataggta gtttcagaca cgacgtgtcc
      541 ctctctcgga accggtggcc gcgcggatct cgatcactac accgccgtgt atgtgttccg
      601 ccatgttgcg ttctctccct ttcgcaaggt cctagtgggt gatcgcttgg gcagccttct
      661 catcctagaa cctcggatac gactgcccag acaattcccc gcgattacac gtcgcggagg
      721 tgcagcttcg ccgtttctct caggaatatg gaagcgacca gagagatcaa tgcgccgccc
      781 attaccatga agcccggcgc caagtcactt cctgtcgcgt ttacaaggta cgtcgccacg
      841 aaaggcgcgg ctccacctgg atctcggtca gtaccgtgcc cgctcctctg ggttcttctg
      901 gagttatgct ttccacaatc ctctactttc cctcaacgtc gacgaatcag gtttcacagc
      961 tacggcgact agtccggatg ccacccccca tccacgtcga tggtctctcc ggtgatgtag
     1021 gtggagcggt cgctgaggag gtagccgacg acgtccgcga tctcctccgg gctggctggg
     1081 cggcccaagg gaacctggga acgcatccgc tgagccaact cggcgtcggt cgtccctacc
     1141 atgcctgcac ctacgtaacc gggcgcgacg ccgttgcatc ggatgccctc cggtgcgagc
     1201 tccctggaca cggagacggt taggttgtgc aacgcggcct tcgacgccgc gtagtgggct
     1261 ccggaaggag agtacggcgg gaaggcccct tctgggccgc tcgcccccag cttagccatt
     1321 atcgagagga tagtgacaat gctcgcccgg ccgtcacggg cgagatgcgg gtaggcctcc
     1381 cggcagagca ggaaggggcc tgtcaggttg accgccatga cttgcgacca ctcttcgagc
     1441 gagatctcgt agaagggccg cttgcggccg tccttcttgg gagagatacc cgtggcaatg
     1501 accagggcgt gcagcacgcc accatctcgg gccgcatcta cggcttcgcg gacgcctttc
     1561 tcagcggtga ggtcgccgac ccaggtgcgt gcggccccag tatcggcgcg cacctgctcg
     1621 gccccctctc ggtcgaggtc tgcgatggtc acctcgtagc ccgcctcgac catctgggca
     1681 actatgtgcc ggccgatacc tccggctcct cctgcgacga gcccgcggac aggtccatct
     1741 ccttcccgtc cttcagtcac gatttcctcc agtttgctcc tatactcgcc ggcactctca
     1801 cggggttgtt tccgtccttg ccgtaccttg gggtttgctc gccaacgcgg cgagcgcgga
     1861 ggccgccgcc ctcctgtcgg tgatgaacgc ggccgccaga cccgccatgc caccgcccac
     1921 gacgagtacg tcgatccggt cgggtagcct ggactgatca gttgtcatcg caactcctcc
     1981 cttgcaccac cgtctttctt ctgtacctcc atagcattct tcatctttgt tggtcagcgt
     2041 aacgacgcgc tgcagcgata acatccgaca tcgcctggcg tgctcgccgg gcgaactcga
     2101 ccggggtcaa agtctctcgc aggggtaaag cgggagcctc aacactaaga ggtatttctt
     2161 ccggcagtgc aacgaggaac tgtgccagcg gcaactcacc atctccgggc aacagtcgca
     2221 tggcccggct ctctagttgc aaatcgctgc cgtccgtaga ttgcccgcga ggtagcctgc
     2281 tggggcgtgg tagattcgta ggtggtcgca ggggcgcgtc gttcagttgc gcgtaggcca
     2341 acaaaccagg attcacggat cgcaagtgtt cgaaactacc gtcgtagcgc tggaaatgaa
     2401 gcgagtccag cagtatcccg ccgccgtcgg agcgttcggc gatgtagacc gcttcatcca
     2461 ggttggatac ctgagtgtaa ataatcggct cgatcagggg ccgcaggcca taaggctttg
     2521 cgtcggcggt tagggttgcg aaggtatcgc acgcccgttc gatatccggg tcgtcgccgt
     2581 tgaccattac gtaactcgct cccaaccggg ctcccgcctg cagcgcgaac tcgtaatctt
     2641 ctcgtctggt ctctggtccg aggcggacaa cctcgacggc cagcgctcgg acaccggtat
     2701 cgtctgaccg gcggatggtt tcctccagca tggaagagcc aacggccatg ggccaaggct
     2761 cttcaccggg ggtcgcggca gcgacgcgga gtcctaccga gtcgaacccc acctcaccgg
     2821 caatggtcac cagctcggga ggcgagatgt ccagcaacgt gagatggtca atgcctactt
     2881 gcggcgatgc attcatttca ttgctcctga cttccgtcta ctttactagc ttctctacgc
     2941 ttcgatcaac gaccgggtcc gaaactcagg gaacgagaat cgccttcacc gcctcgcctt
     3001 ggcccaccac ctcgaaagcc cgcggagcgt cttcgagcgc gtacttcgat agtaggggag
     3061 tggggtctat agtgccttgc cccagcagat ccagagccac tcgatagtct gcctggttgc
     3121 aggagtagct gaacagcagg ctcaactccc tccgcaccgc gagggtgggg gaaaagcgca
     3181 tctcctcggc gtacagaccg accacgataa ccgcccctcc cggccggacg gactccaagg
     3241 cggagccgag cgcctgcacc gatcccgaac tctcgaccca gacgataggc ccctggtctc
     3301 cgaagctatc ctgcaggtgc tcttccaggg acttgtcact cagattcgcg gtccggagcc
     3361 ctacgcgctc ggctgcaggg aggcgggact cggagtcttt ccccactccg gtgaggagca
     3421 cctccgctcc gcgcagttgg gccagcatag cgcagaggat gcctatgggt ccgggtcccg
     3481 acactacaac ctgctgaccg ggctctatgt ccgctcgaac gttcacggcg tgcacggcca
     3541 cagctagcgg ctcgcctagg gccgccacag caaggtccag cccctcggga acaggaatga
     3601 gatgttcttc ggatattacg gcgtattcgg ccatacctcc gtcgcgggag aggccgatgg
     3661 cttcgcgcca gggacacaac tgcggagaac cggcgtgaca aacttcacag cggccacagc
     3721 cctgcaccgc gaccgccact acccggtctc ccgggcttac tccactcacg ccctggccga
     3781 cctcttcgac gatgccggag aactcgtgac ccagcgtcac cggcggctgg acccactcga
     3841 acccg
//
LOCUS       DASTUV010000390         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_367639, whole genome shotgun sequence.
ACCESSION   DASTUV010000390 DASTUV010000000
VERSION     DASTUV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367639"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1004
                     /locus_tag="VFT03_07895"
     CDS             126..1004
                     /locus_tag="VFT03_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU4848106.1"
                     /translation="MKVLLTGATGLLGGALLELLLAEGHEVRCLVREGSPRASRLDVR
                     RAELARGDAADPRALSRALSGVAALLHVAGIEYAPPVVEAARRAGVGRLVVVSSTSAH
                     SAYASRSGPKLRMEGLVRASELDYTIVRPSMIYGSELDKNMHRLLRFLDRSPLYPMFG
                     SGENLWQPVYYDDCARGVYEALVRPTAVGQSYDLPGATPLTYLDLVRTAAGVLGRKPR
                     IIRVPLEPARRVLAAAEALRLPLPVRSEQVLRLREDKAYPYGKARSELGYAPRPFGEG
                     IALEAARLRELRMVRR"
     gene            1001..1972
                     /locus_tag="VFT03_07900"
     CDS             1001..1972
                     /locus_tag="VFT03_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848107.1"
                     /translation="MSETLVAGGVAFLVAGALVPLLVSFAVGRNLLDVPNLRSSHEVP
                     TPRLGGIAIVLGTWAGFALPRPEGAWPLLVAATLVGTVGLADDLSDLHFSTKAAAQTL
                     VAAGLLLFYPPEALSDAPGAWGIAAFAIGVLWLVALANAFNFMDGIDGFIGGVAVINA
                     FFLAALASEAFLPALIGATAGFLVWNISPASIFLGDSGAYFLGFGLAAVALYVPVPGD
                     RWTPLGFLAGVLVFTPLLFDTAYTLVRRLRTGAGKNIFSAHREHIYQRITPSSGMHRR
                     TSNLYYGASVVAGFAALLAQEGGASTLAGIALALVCCLALAALPRIF"
BASE COUNT          289 a          712 c          716 g          366 t
ORIGIN      
        1 tcgagtacgt caccaagaac gacgtgaacc gggctgtctc cgtgcaggtt gtgggagatt
       61 gaacccgacc gcggaaccgg ctggacgcag ccggagacgg gtgtcctcgc gtctctgagc
      121 gtgccgtgaa ggttcttctc acaggggcaa ccggcctgct tggcggcgcg ctactcgagc
      181 tgttgctcgc ggagggacac gaggtacgct gcctggtgcg cgaggggagc ccccgcgcct
      241 cgcgccttga cgtgcgccgc gccgagctcg cccgcgggga cgcggccgac ccgcgcgcgc
      301 tctctcgggc gctctccggg gtggccgcgc tattgcacgt cgcggggatc gagtacgcgc
      361 cgccggtcgt ggaggcggcg cgccgggcgg gggtgggacg cctcgttgtg gtgagcagca
      421 cgagcgcgca ctcggcctac gcttcccggt ccggcccgaa gctccggatg gaggggctgg
      481 tgcgagcgag cgagctggac tacaccatcg tgcgtccgtc catgatctac ggctccgagc
      541 tcgacaagaa catgcaccgc ctcctgcgct tccttgaccg ctcgcccctc taccccatgt
      601 tcggctcggg ggagaacctc tggcagccgg tctactacga tgactgcgcc agaggcgtct
      661 acgaggctct cgtgcggccc acggctgtgg gccagagcta cgacttgccg ggcgccacgc
      721 cccttaccta cctggatctg gtgcggaccg ccgccggagt tctgggaagg aagccgcgca
      781 taatccgggt gccgctcgag cccgcgcgcc gcgttctcgc cgcggccgag gcgctacggc
      841 tgccgctccc ggtccgctcc gagcaggtgc tgaggctgag agaggacaaa gcttatcctt
      901 acggcaaagc gaggagcgag ctcggctacg ctccgcgacc tttcggggag ggcatagcac
      961 tggaggcggc gcgcctgcgg gagctcagga tggtgcggag gtgagcgaga ccctggtagc
     1021 agggggagtg gcgttcctgg ttgcgggagc tctcgtcccg ctgctcgtta gcttcgccgt
     1081 cggacggaac ctgctcgacg tccccaacct gcgcagctcg cacgaggtcc ccaccccgag
     1141 gctcggaggg atcgccattg tgctcggaac gtgggccgga ttcgcgctcc cgcgccccga
     1201 gggggcgtgg ccactgctgg tagcggcgac cctggtcggg acggtcgggc tcgccgacga
     1261 cctctcggac ctccacttca gcacgaaggc agccgcccag accctcgtcg cagcgggact
     1321 cctgctcttc tatccccccg aagcgctctc ggacgctccg ggagcgtggg ggatagcggc
     1381 tttcgccatc ggggtgctct ggctcgtggc tctggccaac gccttcaact tcatggacgg
     1441 catagacggc tttatagggg gggttgcggt tatcaacgcg ttcttcctcg cggccctcgc
     1501 gagcgaggct ttcctgccgg ccctgatcgg ggctacggca ggcttcctcg tgtggaacat
     1561 tagcccggca tcgatcttcc tcggcgactc gggagcatac ttcctcggct tcggcctcgc
     1621 cgcggttgcc ctgtacgtcc ccgtgccggg agacaggtgg acgcccctgg gcttcctcgc
     1681 gggcgtcctc gtcttcacgc cgctcctttt tgacaccgct tacactcttg tgaggcggct
     1741 gcgcacgggg gccggcaaga acatcttctc cgcccaccgc gagcacatct accagcggat
     1801 aacccccagc tctgggatgc acaggcgcac gagcaacctc tattacgggg ctagcgtcgt
     1861 cgccggcttc gcggcgctgc tggcgcagga gggcggagcc tcgacgctcg ccgggatcgc
     1921 cctggcgctg gtgtgctgcc tggcactggc ggctctgcct cgtatcttct aaggagcacg
     1981 ccgcctccct ccggtcggct gcttgtcagc acgcttcggc ccgtctaaag gcccgagcgt
     2041 gctgacaagc ctgcgaagca ggcgtgctaa agtgctgaca aat
//
LOCUS       DASTUV010000391         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_367683, whole genome shotgun sequence.
ACCESSION   DASTUV010000391 DASTUV010000000
VERSION     DASTUV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367683"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFT03_07905"
     CDS             <1..246
                     /locus_tag="VFT03_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEU4848108.1"
                     /translation="EETYYHKIQPLQGFAVQRVYTEDRALDETMTVQDDDTVLVPRGY
                     HAVSTPPGYDLYYLNVMAGPVREWKVKNDPDHEWLLE"
     gene            434..763
                     /locus_tag="VFT03_07910"
     CDS             434..763
                     /locus_tag="VFT03_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848109.1"
                     /translation="MKKTLLLLAMIAVMVLLFAPVATAQYYGGGGGGSTATATAMSTA
                     SPTATATATATTTATATAMSTATATASVLPKSGGPPLVGTVTLLASLALISSGVGALV
                     LVRRSVS"
     gene            1020..2015
                     /locus_tag="VFT03_07915"
     CDS             1020..2015
                     /locus_tag="VFT03_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4848110.1"
                     /translation="MTRVGLLGAGRIGALHGSTLAADPGVERVVVGDVDAERAQALAG
                     RLGSEYGTIEEVMRSRPDAVVIAASTSAHAGLIRDAVDAGIPAFCEKPVALGYEETLE
                     IVEKVESGGAVLQIGFQRRFDAGYVEAKRIVYSGALGTLYSLRLATHDPEPPHEGYIP
                     TSGGIFRDLHIHDFDILRWLAGSEVEEVYAVGSVRGFEVFSRYGDVDTAAALIKMEDG
                     VVAVLTGGRHDPLGYDVRAEVFGSGDSIAVGLDGRTPLRSVESGMPPPEKPYPNFQAR
                     FEDAYRAELRHFLRLARGEAENPCTARDALEALRIAMAADLSLAERRPVRLSEVG"
     gene            2323..>2837
                     /locus_tag="VFT03_07920"
     CDS             2323..>2837
                     /locus_tag="VFT03_07920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU4848111.1"
                     /translation="MELRDLEAYGEPGAVVYLADCVELMRLMPAGSVDVIFADPPYRL
                     STGGVTVRSGRLAPVDKGGWDRSLGSFEKDHAFNVRWLREARRVLKPDGTLWVSGTHH
                     IIFSLGFALQRLEFRVINNVVWSKPNPTPNALHTAFTHAHETLIWASKGRGARHTFNY
                     DLINSPNSEAQL"
BASE COUNT          503 a          911 c          928 g          495 t
ORIGIN      
        1 gaagagactt attaccacaa gatccaaccc ctccagggct tcgccgtcca gagggtctat
       61 acagaagacc gcgcgctcga cgaaacgatg accgtccagg acgacgatac cgtgctcgtg
      121 ccgcggggct accacgccgt ctcaacgccc ccaggctacg acctctacta cctgaacgtc
      181 atggccggcc ccgtgcgcga gtggaaggtg aagaacgacc ccgaccacga gtggctcctg
      241 gaatgatgct cgcgagaggc cgggcgtgac ctcgccgcag gcgtgtgaca cggtagcgat
      301 ccccaacctc acggggtctc aagaggctgg tagcgagcct gttgactcgg gcgttacgac
      361 ttcgcacaat gcaggataag gaaagatggg tagtcgccct ggtcagggca cctaaccggt
      421 aaggaggtgc tgcatgaaga agaccttact ccttctagcc atgatcgccg tgatggtctt
      481 gctgttcgcg ccggtagcca ccgcgcagta ctacggtgga ggcggcggag gaagcacggc
      541 caccgccacc gctatgtcca ctgccagtcc taccgctacc gccacggcta ccgctactac
      601 cactgctact gccaccgcta tgtccaccgc caccgccacc gccagtgtgc tgcccaagag
      661 cggtggaccg ccgctggttg ggacggtcac gttgttggcc agcctggcgc tgatatcctc
      721 cggcgtcggg gcgctggtcc tcgtgcggcg cagcgtttcg taacacgcta tagccttgtg
      781 ttgatgacct cataggcaga tagcccatct ttccccgtgc cggggtccgt agtggaggga
      841 ccccggctct tcacttgggc gcgccgggcg cgagatcctc cccggttccc accgcagccc
      901 ctgttcgcat cttgacagag caccggcgga tttgtaccgt ccgtaactga ttcgagtgct
      961 ccaatgaggt gtgggaaagg atcgtggcac gtcgggctac tcgtgctgat agaaaggtgt
     1021 tgacgagggt cgggttgttg ggggcgggta ggatcggtgc cctgcacggc agtacgctcg
     1081 cggctgaccc tggcgtcgaa cgcgtggtcg tcggcgacgt ggacgccgaa cgagcgcagg
     1141 cgctcgcagg gaggctgggt agtgagtacg ggaccatcga ggaggtgatg cgttccaggc
     1201 ctgacgcggt ggtgatcgcc gcctccacct ctgcgcacgc gggcttgatc cgtgatgctg
     1261 tggacgcggg gatcccggcc ttctgcgaga agcctgtagc tctcggctac gaggagacac
     1321 tagagatcgt cgagaaagtc gagtccggcg gcgcggtgct ccagatcggc ttccagagga
     1381 ggttcgacgc cgggtacgtc gaggccaaac gtatcgtata tagcggtgct ctcgggacgc
     1441 tgtactcgct acggcttgcg acccacgatc ccgagccgcc gcatgaaggg tacatcccga
     1501 cctcgggcgg catcttccgc gacctgcaca tccacgactt cgacatcctg cgctggctgg
     1561 caggcagcga ggtcgaggag gtctatgccg tgggcagcgt gcgcgggttc gaagtcttct
     1621 ccaggtacgg ggacgtggac accgccgcgg ctctcatcaa gatggaggac ggcgtggtgg
     1681 cggtattgac cggcgggcgc cacgaccctc tgggatacga cgtgcgggcc gaggtcttcg
     1741 gctctggcga cagcattgcc gtcggtctgg acggacgcac gcctctgcgc tctgtggagt
     1801 cggggatgcc gccgccagag aagccgtacc cgaacttcca ggcgcgtttc gaggacgcct
     1861 acagggccga gctcaggcac ttcctgcgcc tcgcccgcgg agaggccgaa aacccctgca
     1921 ccgcacggga cgccctggag gcgctccgga tagcgatggc cgccgacctc tcgctcgcgg
     1981 agcggcgtcc cgtgcggttg tcggaagtcg gatgaagatg cgcgtgtctt agcctggcgc
     2041 cggggagtag aaaaggggcc ggtggggcct cgagggaaag tcgacgaggc cgggctcccg
     2101 gtccccgagg atgtctacgt cgtcgactac gacaacacgt atcttgccga gctcgccctg
     2161 gtctcgttga ctcgccgctc gcctcctgct cgagcgtgtc gagtcggaac gtaccgaacc
     2221 tcgtcgcgag gtgctcaggc cgcgcctgat ccggcgctcc acctccgccc ctccggcagc
     2281 tcccggctaa agcctcaccc ccatccgtcc aacaataacg gcgtggagtt gagggacctc
     2341 gaagcctacg gcgagcctgg tgccgtcgtc tacctcgcag actgcgtgga gcttatgcgc
     2401 ctcatgccgg cagggagcgt cgacgttatc ttcgccgacc ctccctaccg gctctcgacg
     2461 ggcggggtga ccgtgaggag tggtcgtctt gctcccgtgg acaagggagg ctgggaccgg
     2521 tcgctcggca gcttcgagaa ggatcacgcg ttcaacgtga ggtggctgcg cgaagcgcgg
     2581 cgcgtcctca agccagacgg cacgctctgg gtgagtggaa cccaccacat catcttcagc
     2641 ctcgggttcg cgctgcagcg gctggagttc agggtcataa acaacgtcgt atggagcaag
     2701 ccgaacccaa ctcccaacgc cctgcacacc gccttcaccc acgctcacga gacattgatc
     2761 tgggcgagca agggaagggg agcacgccac accttcaact acgacctcat aaacagcccc
     2821 aactccgaag ctcagct
//
LOCUS       DASTUV010000392         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1729545, whole genome shotgun sequence.
ACCESSION   DASTUV010000392 DASTUV010000000
VERSION     DASTUV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729545"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="dnaX"
                     /locus_tag="VFT03_07925"
     CDS             complement(<1..1195)
                     /gene="dnaX"
                     /locus_tag="VFT03_07925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU4848112.1"
                     /translation="MLREPYSPLIHMNHTTLYRKWRPRRFGQVVGQEPVVRTISRAIE
                     TGRVAHAYLFSGPRGTGKTSTAKVLAMGLNCAQGPTPEPDGTCESCRAIMNNSSLDVV
                     EMDAASNRGIDEIRDLRDRVNLAPVAGRMKVYIIDEVHMLTPEAFNALLKMLEEPPEH
                     VVFVLATTEKHRVLPTIISRCQSFDFRRPGVETLKEKLAEIAEAEGIPVEPEALTVIA
                     REGRGSFRDAEGLLDQLSSFAESQITASMVRELLGSVGPETLLETTLALYERRAADAL
                     RIVDRLSNQGRDLGQFVGELISHLRTVMLLPHAPEVALAEVGADERGPLEEQAQTIPT
                     AEVVRLIEALDGALGRIKRGGDPRLELELSFLRVTRDYTEPSVERLLGRLEALEQAAE
                     KGSLAG"
     gene            complement(1280..1544)
                     /gene="ffs"
                     /locus_tag="VFT03_07930"
     ncRNA           complement(1280..1544)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFT03_07930"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(1661..1942)
                     /locus_tag="VFT03_07935"
     CDS             complement(1661..1942)
                     /locus_tag="VFT03_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848113.1"
                     /translation="MITVTEEAKDLFMNVERPDGTVLRLDPVVDQDSGETQIGISAGE
                     PMSGDQVVEHQGETLLYIAAPVSEALDGSTLDAVDTPQGPGIGIITPDS"
     gene            complement(2161..2871)
                     /locus_tag="VFT03_07940"
     CDS             complement(2161..2871)
                     /locus_tag="VFT03_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEU4848114.1"
                     /translation="MPQPIELYFWPTPNGKKITIFLEEAELPYNLAPVDITAGEQFDE
                     EYLRISPNNKMPAIVDPEGPDGGPISVFESGAILVYLAEKTGRFMPSSPRQRYEALQW
                     LMFQVSSVGPMLGQAHHYRGYAPEEIPYAVDRYTNEAARLYGVMDARLSEVKYFVGDE
                     YSIVDMAIYPWVAPYERQGQRIEDYPNLRRWYEEMAERPALRRAMEVGEELRRPIEDL
                     DEEARDVLFGGRPRTTGA"
BASE COUNT          522 a         1020 c          996 g          632 t
ORIGIN      
        1 ctccggccag gcttcccttt tcggccgcct gctccagcgc ctccagacgg cccaggagcc
       61 tctcgaccga gggctcggta tagtcgcggg tcactctgag gaaggagagc tccagctcga
      121 gcctgggatc cccgccgcgc ttaatgcgcc ccagagcgcc gtccagcgcc tcgatgagcc
      181 gcacgacctc tgccgtcggg atggtctgcg cttgctcctc gagcggcccg cgctcgtcgg
      241 ccccgacctc ggcgagagcg acctcggggg cgtgcggcag gagcatcacg gttcgcaggt
      301 gcgagatcag ctccccgacg aactggccga ggtccctgcc ctggttcgag agccggtcca
      361 caatgcgcaa agcgtctgcg gccctgcgct cgtagagagc taaggtcgtc tcgaggagag
      421 tctcggggcc gacgctcccg agcagctcac gcaccatcga agccgtgatc tggctttccg
      481 cgaaagagga gagctggtcg aggaggccct cggcgtcgcg gaaggagccc ctgccctcgc
      541 gggctatgac cgtgagcgct tccggctcta ccggtatgcc ctcggcctcc gctatctcgg
      601 ccagcttctc tttgagcgtc tcgacgccgg gacggcggaa gtcgaagctc tggcagcggc
      661 ttatgatggt ggggagcacc ctgtgcttct ctgtagtggc gagtacgaag acgacgtgct
      721 cggggggctc ctcgagcatc ttcaggaggg cgttgaaggc ctcgggagta agcatgtgga
      781 cctcgtctat gatgtagacc ttcatcctgc cggccacggg ggcgaggttc accctgtctc
      841 tgaggtcccg aatctcatct atcccccggt tcgaagccgc gtccatctcg accacgtcga
      901 gcgaggagtt gttcatgatc gcgcggcagc tctcgcacgt cccgtccggc tccggcgtcg
      961 gcccctgagc acagttcaac cccatcgcga gcaccttcgc cgtgctagtc tttccagtcc
     1021 ccctgggtcc agagaacagg taggcgtggg cgacgcggcc ggtctctatg gcccggctga
     1081 tggtacgcac taccggctcc tggccgacga cctggccaaa acgcctgggc cgccacttgc
     1141 ggtagagcgt ggtgtggttc atgtggatga gagggctgta aggctcacgc agcaagtata
     1201 ccggacgacc cggcgaggac catcggtgca cggcgcacca cggcaacatc cgctaagaag
     1261 gtctccgcta agaaaaatgc cgcgcacccc gccgtcgacg ggctcgaacc cggcgaactc
     1321 ccggctgcta gctccggccg agctaccccg aagcacatga ggttacccgc ttagtgctgc
     1381 tacctcccgg tcctgacacg gttcacgggg cctcattgcc cgggacccgg ccatcaacgc
     1441 cgcggtcacc ggtctctacc gaacacgggc cgcctcgggc gggggtctag gccctgctag
     1501 gcgatcgcag gtacagggca tcgccagctc cccgcttagc gcggcggagt gattttatca
     1561 gagaggctcg aagttacagg cctcgggcat caggaattac ctgattacct gtagattgaa
     1621 atcggctgct tgaaaagcgt cgcgagactc ctctggccac ttacgagtcg ggagtaatga
     1681 tgccgatgcc cggtccctgg ggggtgtcga ccgcgtccag ggtgctgccg tcgagcgcct
     1741 cgctcacggg ggccgcgatg tacagcaggg tctccccttg gtgctcgact acctgatctc
     1801 cgctcatcgg ttcgccggcg gagatgccga tctgggtctc tccgctgtcc tggtctacca
     1861 cggggtcgag acggagcact gtaccgtcgg gacgctcgac gttcatgaag agatccttcg
     1921 cctcttcggt taccgtgatc atgcccttct cctttcctcg ccgcagcggg cgatagagcg
     1981 gagcgtgcag tgtcttcgcc gaccctacca ggtacgcgca agatttaaac ctggagtctg
     2041 aacttgcgct tcggagtgtt cgcgcgagta agtgtctcca agtgtgcgct tctgcaccgt
     2101 gtctcctttc gacaagtcga tagctacgtt cttccgcgca gcgcgaagtc tcttgggaga
     2161 ctatgctccc gtggtgcgag gcctgccccc gaagagcacg tcgcgggcct cctcgtcgag
     2221 gtcctctatc gggcggcgca actcctctcc cacctccatc gcccgtcgca acgctggccg
     2281 ctctgccatc tcctcgtacc agcgcctaag gttcgggtaa tcctcgatcc tctggccctg
     2341 tctctcgtag ggggcgaccc agggatagat ggccatgtcc acgatcgagt actcatcgcc
     2401 gacgaagtac ttgacctcag agaggcgagc gtccatcacc ccgtagaggc gggcggcctc
     2461 gttggtgtag cgatctacgg cgtaggggat ctcctcgggc gcgtatcctc ggtagtggtg
     2521 ggcctgtcct agcatcggtc ccacggagct cacctggaac atgagccact gcaacgcctc
     2581 gtatcgctgc cgcggcgatg atggcatgaa cctgccggtc ttctcggcga ggtagacaag
     2641 aatcgctccc gactcgaaga cggatatggg tcctccgtcg ggaccttctg gatccactat
     2701 ggcgggcatc ttgttgttgg ggcttatcct gaggtactcc tcgtcgaact gctcaccggc
     2761 cgttatgtct actggtgcca ggttgtaggg gagctcggcc tcttcgagga agatggtgat
     2821 cttcttgccg ttgggcgttg gccagaagta gagctctatg ggctgtggca tgcggcctct
     2881 cgctcgtcgg tagtgcgtcg cgacgcatat tgtagcgggc cccgtgtccc tcgtttagta
     2941 cgataccccg tgggctgtag ccaccatgcg gtctacctcg aaaagcgtct tgatctcgcc
     3001 agggtacaga gagctgagta gcgtgagtaa gcgaggtaga cggatgtaac cggcatcagg
     3061 agcccgccgg tgcggctcag cactgtgtgt cagacgacaa tcatctctag aggctgcttg
     3121 gagaggactt ctcgccccgt ctccgtaacc aggcacgtgc ggcccaagct
//
LOCUS       DASTUV010000393         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_895353, whole genome shotgun sequence.
ACCESSION   DASTUV010000393 DASTUV010000000
VERSION     DASTUV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895353"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFT03_07945"
     CDS             <1..380
                     /locus_tag="VFT03_07945"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEU4848115.1"
                     /translation="SRLNNVGEMEAEMASIREKIEGEVAGWEGVEARPHRFGGVEFRV
                     RGHEIGHLHGSRLADLPFPVRMRKELVASGKARLHHVLPETGWVSYPIRGEEDVEGAL
                     ELFRLNYERLTARGEKETVVEGS"
     gene            377..790
                     /locus_tag="VFT03_07950"
     CDS             377..790
                     /locus_tag="VFT03_07950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU4848116.1"
                     /translation="MSAFTEKEIEYLRERRLGRLATVNVKGEPHVVPVGFRYNEEMDA
                     IDIGGHNFARSKKYRDVRDTGRAAFVVDDVLPPWRPRGVEVRGRAEVHGEGGEEIGPG
                     FDADVVPIFPKRIVGWGLDSDAFRPNSRTVEMESA"
     gene            787..1233
                     /locus_tag="VFT03_07955"
     CDS             787..1233
                     /locus_tag="VFT03_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU4848117.1"
                     /translation="MIRVHPIKESTGYALAKVCKAHRGNVGEYLAEVGLHVGQEMVLI
                     ELFEQDGLRGGDLAERLGVEPPTVTKMLRRLEKCGLVERRRDSRDARSFRVYLTGEGR
                     SLEEPLARCWEKVEERTFAGMSIGERQTFRRLLAKVRSNIDLGCAE"
     gene            1329..2744
                     /locus_tag="VFT03_07960"
     CDS             1329..2744
                     /locus_tag="VFT03_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848118.1"
                     /translation="MSAGTEKKTSTRLLLAVLTSAVFVSVLNSSMVNVAIPLIKEDFG
                     ASEAQVGWVITGFLLVYAIGIPLYGRAADIFSLRRTFTLGLMVFALGSLICIVAPSLP
                     LLVFGRIVQAAGGAAVPALASASVVKVLPPGDRGTALGLIVSSVGVGAAVGPVVGGTL
                     VQLAGWHSLFYGVLFLILILIPSALYALPEIETTGERGFDVPGGVLLGLAAGLFLFGI
                     TQGQVAGFGSPASWGSFAGAALAAAGFAWRITSAENPFVSPGLFENRRYVAAVIVGYF
                     SMLVNVSCLVSVPLLVSQVNGLPPGATGLVLAPGAIALAILSPLAGRLSDRIGFKVPV
                     YAGLTIMLLSVLFLSTFAAGGSPYLVALGMLGVGTGFALTNSPNVNAAAAALPPEETG
                     VGLGIFNGLFFLGGGTGPAVVGAFLAARKEAGAGAIDPLYQLGAAPFSDAFLLLSVAL
                     LLALAAALWLPDGSNAQVPEG"
     gene            2753..3091
                     /locus_tag="VFT03_07965"
     CDS             2753..3091
                     /locus_tag="VFT03_07965"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEU4848119.1"
                     /translation="MDSNALAVEREVLRWPGVTSEPGRFGATSFRYGKREIGHIHRDR
                     IADLPVTTGMREELLSRGRARPHRAGVKGYISHPLENEEDVSAVLEILGRNYDRAKDA
                     AERRAASTEG"
     gene            3094..>3797
                     /locus_tag="VFT03_07970"
     CDS             3094..>3797
                     /locus_tag="VFT03_07970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU4848120.1"
                     /translation="MADYGRDLEFGASVAPLADPPDFAQRVSVAADRAGLDLVGIQDH
                     PYQRRFLDTWTLISTLVPVTERIRFFPDVACLPLRPPAVLAKSAASLDVLSGGRVEMG
                     LGAGNFWDAIVAMGGPRRSPSEAVRSVEEAIQVMRLVWSEERSVRFDGEIYALKGLKP
                     GPHPVHEIGIWVGAGGPRMLGLIGRLADGWVPSMSWATPERLPEMHQRIDEGAASAGR
                     DPAEIRRVYNISGVIG"
BASE COUNT          610 a         1133 c         1400 g          654 t
ORIGIN      
        1 ttagccggct aaacaatgtc ggtgagatgg aggcagagat ggcgagcatc agggagaaga
       61 tagagggtga ggtagcgggt tgggagggag tcgaagcgcg gccgcaccgg tttggtgggg
      121 tggagttccg ggtgaggggg cacgagatcg ggcacctgca cgggagccgg ctggcggacc
      181 ttccgtttcc ggttcggatg aggaaggagc tagtggcgtc gggcaaggcc agattgcacc
      241 acgtcctgcc ggagacgggg tgggtgagct atccgatacg gggcgaggag gacgtcgagg
      301 gggcgctgga gctgttcagg ctcaactacg agcggctgac tgcgcgtgga gagaaagaga
      361 cggttgtgga ggggtcatga gcgcgtttac ggagaaggag atcgagtacc tgcgggagcg
      421 gaggctgggg cggctggcta cggtgaacgt gaagggtgag ccgcacgtgg tacccgtggg
      481 gttccggtac aacgaggaga tggatgccat agacatcggc ggccacaact tcgccaggag
      541 caagaagtac cgcgacgtca gagacacggg gcgggcggcg ttcgtggtgg acgacgtgtt
      601 gccgccctgg cggccgcggg gcgtggaggt cagggggcgc gcggaggttc atggggaagg
      661 cggagaggag atcggcccag gcttcgacgc tgacgtggtc ccaatcttcc cgaagcgcat
      721 cgtaggatgg ggactggact ccgacgcttt caggccgaac agccggacgg tcgagatgga
      781 gagcgcgtga tacgggtcca cccgatcaag gagagcaccg gctacgcgct cgcgaaggtc
      841 tgcaaggcgc accgggggaa cgtcggggag tacctggccg aggtcggcct tcacgtcggc
      901 caggagatgg tcctcataga gctcttcgag caggacgggc tgcggggcgg ggacctcgcc
      961 gagagactcg gcgtcgagcc gccgaccgtc acaaagatgc tgcggcgcct cgagaagtgc
     1021 ggcctcgtgg agcgccgccg ggactcgcgg gacgccagga gcttccgcgt gtacctcacc
     1081 ggagagggac gctcgctcga agagccgctc gcccggtgct gggagaaggt tgaagagagg
     1141 acctttgcgg gtatgagcat cggggagcgg cagacatttc gccggctcct ggccaaggtg
     1201 cggtccaaca tagatctggg ctgcgccgag tagagtagat accattttac aggcgatctt
     1261 tttcaggaag aggagcggat ggcttttaga gacggtcgtt tgaagattct gggaaccggg
     1321 ggcacgctgt gagcgccggc acggaaaaga agacgtccac ccggctcctc ctggcggtgc
     1381 tcacctccgc cgtattcgtc tcggtgctca acagctcgat ggtgaacgtc gccattccac
     1441 tcataaagga ggacttcggg gcgtctgagg cgcaggtcgg ctgggtcata accggcttcc
     1501 tgctcgtcta tgccataggc atcccgctct acggacgcgc cgccgacatc ttcagcctgc
     1561 ggcgtacttt tacgctggga ctgatggttt ttgctctcgg gagtctcatc tgcatcgtcg
     1621 cgccgagcct gccactgctg gtattcgggc gaatcgtgca ggcggcgggg ggcgcggccg
     1681 taccggcgct agcgagcgct tcggtggtga aggttctgcc gccgggcgac cgtgggacgg
     1741 cgctgggtct gatcgtgtcg agcgtaggtg tcggggccgc cgtcgggccg gtggttggcg
     1801 gcacgctcgt gcaactggcc gggtggcact cgctcttcta cggcgtactg ttcctgatcc
     1861 tgatcctgat ccctagcgcg ctctacgcgc tgccggagat cgaaaccacg ggcgagcgcg
     1921 gattcgacgt gcccggcggt gttctgctcg ggctggcggc gggcctgttc ctgttcggca
     1981 taacgcaggg gcaggtggcg gggtttggct ctcctgcttc ctggggcagc ttcgcggggg
     2041 cggcgctcgc ggccgcaggc ttcgcctggc gaatcacgag cgccgaaaac cccttcgtgt
     2101 ctcccggcct cttcgagaac cggaggtacg tggcggcggt gatcgtgggg tacttctcca
     2161 tgctggtcaa tgtctcttgc ctggtctccg tcccgctcct cgtctcgcag gtgaacggac
     2221 tcccaccggg agccacgggg ctggtgctgg cgccgggggc gatcgcgctc gcgatcctct
     2281 ctcccctggc tggacgtctc tcggaccgga tcggcttcaa agtgcccgtc tacgccgggc
     2341 tgacgatcat gctgctctcc gtcctcttcc tctccacgtt cgcggccggg ggatctccct
     2401 acctcgtcgc gctggggatg ctcggggtcg gaaccgggtt cgcgctcacc aactccccga
     2461 acgtgaacgc ggcagccgca gcgctgcctc cggaggagac aggggtgggc cttggtatct
     2521 tcaacggcct cttcttcctg ggcggcggga ccggtcctgc ggtcgtcggg gcgttcctcg
     2581 cggcgcgcaa agaggccggt gcaggagcga tagaccccct ctaccagctc ggcgccgcac
     2641 ccttctcgga cgccttcttg ctcctgagcg tcgcgctgct gctcgccctg gcagccgcgc
     2701 tctggttgcc ggacggcagt aacgcgcagg tccccgaagg ttaggaggaa ccatggacag
     2761 caacgctctg gcggtcgagc gcgaggtgct ccgctggccg ggggtgacct cggagccggg
     2821 aaggttcggc gctacctcgt tccgctacgg gaagcgcgag atcgggcaca tccaccgcga
     2881 ccggatagcc gaccttcccg tcaccaccgg gatgcgcgaa gaactactct cgcggggccg
     2941 cgcacgcccg caccgggccg gggtaaaagg ctacataagc catcccctgg agaacgaaga
     3001 agacgtctct gcggtgctgg agatcctggg ccggaactac gaccgggcga aggacgcagc
     3061 ggagcgccgc gcggcgagca cggaggggta acgatggccg attacggcag ggatctggag
     3121 ttcggggcga gcgtcgcgcc gctggccgac ccgccggact tcgcgcagcg ggtctcggta
     3181 gcggcggacc gggcgggact ggacctcgtc gggatacagg accatcctta ccagcgcagg
     3241 ttccttgaca cctggaccct catctccacg ctcgtccccg tcacagagcg gatacgcttc
     3301 ttccccgacg tggcctgtct gcccctgcgt ccgcccgccg tgctcgccaa gtcggcagcc
     3361 tcactcgacg tgctctccgg cggccgcgtg gagatgggcc tgggcgccgg caatttctgg
     3421 gatgcgatag tggcgatggg tggtccgcgt cgtagtccca gcgaggcggt ccgctccgtc
     3481 gaggaggcta tacaggtgat gcgcctggtg tggagcgagg agcgctccgt gcgattcgac
     3541 ggggagatct atgctctcaa gggcctgaaa ccagggccgc atcccgtcca cgagataggg
     3601 atctgggtcg gagcgggagg tccgcggatg ctcggcctca taggtcggct cgccgacggc
     3661 tgggtcccct cgatgagctg ggcgactcca gagaggctgc ccgagatgca ccagcgaata
     3721 gacgaaggcg ccgccagcgc gggcagagat cctgctgaga tccggcgcgt ctacaacatc
     3781 tcgggcgtga tcggcga
//
LOCUS       DASTUV010000394         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_978362, whole genome shotgun sequence.
ACCESSION   DASTUV010000394 DASTUV010000000
VERSION     DASTUV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978362"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFT03_07975"
     CDS             <1..466
                     /locus_tag="VFT03_07975"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU4848121.1"
                     /translation="EALSFAGPSFRDLTRVGASNPSLWSDILAENAPALGEALGAFAG
                     AMAQLGSEIKDREAMEQRFLQAREAYDALGGILVERSGENVELAVPVENRPGVFAEVT
                     TLMGSNNINILDLYVRHSNTERAALILTLNSDAAPLARELLRDAGFGAEVQG"
     gene            complement(553..1443)
                     /locus_tag="VFT03_07980"
     CDS             complement(553..1443)
                     /locus_tag="VFT03_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU4848122.1"
                     /translation="MKVLISGATGLIGSSLIRELEDEGHSITRLIRSPEGENDVRWDP
                     DAGTIDGSLEGHDAVVHLAGESIAEGRWTAARKQRIMQSRKKGTRLLAEAIARLSVSP
                     EVMVSASAVGYYGDRGNELLREDSGPGSDFLAEVCEAWEAAADPARGAGIRVVHPRFG
                     LVLSPQGGALGTTLPIFKLGGGGRIGSGRQWWSWVTLDDVVGAVRHALENDSVEGPVN
                     VGSPNPLTNAQYTKVLGKVLNRPTFFAVPAPAIRIAIGEMADALLLASQRMEPARLKE
                     TGYHFRYPELEGALRHLLGR"
     gene            complement(1553..2542)
                     /locus_tag="VFT03_07985"
     CDS             complement(1553..2542)
                     /locus_tag="VFT03_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4848123.1"
                     /translation="MNVSIILLGVLALVVFLVAARSVRIIPQSRVAIVQRLGRYHRTA
                     QSGLTFVVPVVDRMLPKTDLREQVVAFQPQAVITNDNVGMQISTVVYYQVVDARAAEY
                     EVANFHLALEQITQTTLRNVIGNLILDKTLTSRDEINAKLRTVLDEVTENWGIRVTRV
                     ELKEIIPPRDIQQAMEKQMQAERTRRAAILTAEGDKRAAILKSEGEKESAILRAEGDQ
                     RAAVLRAEGEAQAYRNVQAAQIEMTGKLFERLESSDLSPESLRYLYLRALPEMAKGPA
                     SKLFVVPSEIQDLAGAIGTLAGGASLANEDGAEAEQRQLGESARRGPASLPRE"
     gene            complement(2539..3006)
                     /locus_tag="VFT03_07990"
     CDS             complement(2539..3006)
                     /locus_tag="VFT03_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU4848124.1"
                     /translation="MENLDIVFWAVLAALAFVGELLTVSFFLLFFSLGALVGLIAASL
                     GFGIAAQAIGFVAASVLSMAILRPALVHRISFRSSEPYESRDAVTGKSGVITDRIEPG
                     GSGTVRIGSGEFWTARSVNPGQGIEAGARVRVLDTDGVTALVEAVGIEEGEKW"
BASE COUNT          525 a         1082 c         1037 g          571 t
ORIGIN      
        1 cgaggcgctc tccttcgccg ggccctcgtt cagggacctc acccgggtgg gggcgtccaa
       61 tccttccctc tggtcggata tcctggcgga gaacgctccg gctctggggg aggcgctcgg
      121 ggctttcgcg ggggcgatgg cacagctggg gagcgagatc aaagaccgcg aggccatgga
      181 gcagcgcttc ttgcaggccc gcgaggccta cgacgccctc ggcggcatcc tcgtcgagag
      241 gagcggggag aacgtcgagc tggccgtccc ggttgagaac aggccaggcg tcttcgccga
      301 ggtgacgacc ctcatggggt cgaacaacat aaacatcctc gacctctacg tccgccactc
      361 caacaccgag cgcgcagctc tgatcctgac gctgaactcc gacgccgcgc cgctggccag
      421 agagttactc cgcgatgccg gcttcggggc ggaggttcag ggatagctgt ggcttgtcgc
      481 tctcggcttc gccgctgcac taggtgacgg gatatctgcg caatattcct cgcggctgat
      541 tgtggagtaa tctcacctcc cgagaaggtg tcgcagggcg ccttcgagct ccgggtaacg
      601 gaagtggtag ccagtctcct tcagcctcgc gggctccatg cgctggctcg cgaggagcag
      661 agcgtcggcc atctcgccga tggcgatccg gatggccgga gccgggacgg cgaagaacgt
      721 cggacggttc aggaccttcc ccagtacttt cgtgtactgg gcgttggtga gggggttggg
      781 gctcccgacg tttacggggc cctcgacgga gtcgttctcg agcgcgtgac ggacggctcc
      841 gacgacgtcg tcgagagtta cccagctcca ccactgccgg ccgctcccga tcctgcctcc
      901 cccgcccaac ttgaagatgg gaagggtcgt gcccagagct ccaccttgcg ggctcaagac
      961 gagcccgaag cgcgggtgga cgacgcggat gcccgccccc cgcgcggggt cggccgccgc
     1021 ctcccacgcc tcgcacacct cggcgaggaa atccgagccg ggcccactat cctcgcgcaa
     1081 gagctcgttg cccctgtcgc cgtagtagcc gaccgcagac gcgctgacca taacttcagg
     1141 cgacacggag agtctcgcga tggcctcggc gagcaggcgc gttcccttct tgcggctctg
     1201 catgatgcgc tgtttcctgg cggcggtcca gcgaccctcg gcgatgctct cgccggcgag
     1261 gtggactacc gcgtcgtggc cttcgaggga gccatcaata gtgccggcgt ccgggtccca
     1321 acggacatcg ttttcccctt caggggaacg gatcaggcgg gtgatgctgt ggccttcgtc
     1381 ttcgagctcc cgaatgaggg aggacccgat caggcccgtc gccccgctga taagaacctt
     1441 catctgcttc tccctgcttc gccgcgctcc gaatggtacc ggtcacagtc gcgcagggat
     1501 atgaacgcag ccacacgttg gcccgcccgc acgggttcgg acacgagcgg cgttattctc
     1561 ggggcaggct agccgggccg cggcgcgcgg actctccgag ctggcgttgc tcggcctccg
     1621 cgccgtcctc gttggcgagg gaagctccgc cggccagggt gcctatggca cccgcgaggt
     1681 cctgtatctc ggaggggacc acgaagagct tgctcgccgg acccttagcc atctcgggga
     1741 gcgctctgag atagaggtag cggagggact cgggggagag atccgagctc tcgagccgct
     1801 cgaagagctt tccggtcatc tcgatctggg cggcctggac gttgcggtaa gcttgcgctt
     1861 cgccctcggc gcgcagaacg gcagcccgct ggtctccttc tgccctgagg atggccgatt
     1921 ccttctcgcc ctcggacttc aggatcgcgg cgcgcttgtc tccctcggcg gtcaggatcg
     1981 ctgccctgcg cgtgcgctcg gcctgcatct gcttctccat cgcctgctgg atgtccctgg
     2041 gagggatgat ctccttcagt tcgacgcgcg tcaccctgat gccccaattc tccgtgacct
     2101 cgtcgaggac cgtgcggagc ttggcgttga tctcgtcgcg ggaggtcagg gtcttgtcga
     2161 ggattaggtt cccgatgacg ttgcgcaacg tggtctgggt gatctgctcg agcgccaggt
     2221 ggaagttggc gacctcgtac tctgcggccc tggcgtcaac gacctggtag tagacgacgg
     2281 tggagatctg catcccgacg ttgtcgttcg ttatgaccgc ctggggctgg aaggcgacga
     2341 cctgttccct gaggtccgtc ttgggcagca tccggtctac cacggggacc acgaacgtga
     2401 gcccgctctg cgcggtccgg tggtagcgcc ctagccgctg cacgatggcg acccggctct
     2461 gcgggatgat cctcacgctc ctcgccgcta ccaggaacac gacgagcgca agcacgccaa
     2521 gcaagatgat gctaacgttc accacttctc tccttcctct atgccgacgg cctcgacgag
     2581 ggccgtgaca ccgtccgtgt cgaggaccct tacccgagcc cccgcctcta tgccctgccc
     2641 cggattgacc gagcgggccg tccagaactc cccgcttcct atccgcacgg taccgctgcc
     2701 cccaggctct atccgatccg tgatgacccc gctcttgccc gtgacggcgt cgcggctctc
     2761 gtagggctcg ctactccgga aggagatcct gtgcaccagc gccggcctta aaatggccat
     2821 gctcaagacc gaagcggcga cgaagccgat ggcctgcgcc gcgatcccga accccaggga
     2881 agccgcgatc agcccgacca gcgctcctaa cgagaagaag agtaggaaga aggaaacggt
     2941 gaggagttcc cccacgaagg cgagggcggc cagcactgcc cagaagacga tgtccaggtt
     3001 ctccaaatcc ggtctcctcc cccacattct ataccagccc ggaccgcaac gacgctccgc
     3061 gaacgcccag aagacgatat cctcaggccc gggaagagcc gcgttagcga cggtgccagg
     3121 atcatcccgg cgccagggga gacgtcaacc cggaaaggag cttcctggat gacaccggca
     3181 cagcactacg acgctgtcgt gataggctcc ggcca
//
LOCUS       DASTUV010000395         5469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1439628, whole genome shotgun sequence.
ACCESSION   DASTUV010000395 DASTUV010000000
VERSION     DASTUV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439628"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFT03_07995"
     CDS             complement(<1..650)
                     /locus_tag="VFT03_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4848125.1"
                     /translation="MEGNRRKRIAIACQGGGSHTAFTAGALKTLLQDQRYEFVALSGT
                     SGGAVCALLAWYALLEHGQAEEGASKAAAEAARSLEKFWLEDNAARNAPEKLLNDWLV
                     GWLRWQQTTGFLFESSPNAFSDYWQQRLREILEDNVPFGDIDGDLVRPASPKLFVGAV
                     NILTGEFKVFGSHRRVDARDDPDGRASWVFNDDPEDGIGVGAVLASAAIPPIFRSIT"
     gene            762..1661
                     /locus_tag="VFT03_08000"
     CDS             762..1661
                     /locus_tag="VFT03_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4848126.1"
                     /translation="MRIFGKRRFVVLAFALLVLFWGSAFAVVKVGLDHSPPVLFAGLR
                     ALIGGVAMVIAALLWGGNPNLRRDWPVFLLLSLFNVVLFIGFQTYAIMHLPSGSAAVL
                     VYLQPILVGLLAWPLLGETLTATKVFGLLLGFSGIVAVSAGSFSGTAAALSPLGVVLG
                     ASSALFWALGTVFFKKHEERVSTLWAVALPFLVGGMVLTAFGLFVESWSEVSLNGVFV
                     ASLLYSGLVGISLAWVIWFALVRAGEASRVASYIFAVPLTAVLIGVVFLDEPLGYTLL
                     IGAAFVVSGIYLVNREPVNKTKE"
     gene            complement(1742..2605)
                     /locus_tag="VFT03_08005"
     CDS             complement(1742..2605)
                     /locus_tag="VFT03_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4848127.1"
                     /translation="MTSPEEMSAKHEAARQQSRATWNDVAPGWYAQREKLWEASRPIS
                     EWLVRRLDPQPGDTVLELAAGLGDTGLMAARLVGESGRVVITDFAPEMVAAARQRAQE
                     LGVKNAEFRLLDAERMDLETDSVDGVLCRWAYMLMTDPAAAFAETRRVLRPGGRLAFS
                     VWASRERNPALSLVGRVLESQGHIPPPEPGAPGAFAMADPGRIQELLIGASFAEPEVE
                     EVSFRWSFAEQEAYWRYLTETSASAAPVLRSLPTEAQEEIREQVHEVALPYHSGEGYD
                     FPAVCLNVVTY"
     gene            2984..3388
                     /locus_tag="VFT03_08010"
     CDS             2984..3388
                     /locus_tag="VFT03_08010"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4848128.1"
                     /translation="MPPFNEETARQKVKAAQDAWNTRDPERVALAYTEDSRWRNRDEF
                     FEGRDAIKEFLRRKWARELDYRLEKNLWCFTGNRIAVRFEYESHDAEGQWWRSHGNEL
                     WEFDENGLMQRRDASINDYKIDESERKFRWTR"
     gene            3462..3749
                     /locus_tag="VFT03_08015"
     CDS             3462..3749
                     /locus_tag="VFT03_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEU4848129.1"
                     /translation="MIDHLVFLAANEDASPEDIEDLISSIRGLKDTVPGVVDLSVGEN
                     FSPRSGGYTHGLFVRFETADNLQGYLEHPDHQAVAEKLDRLTSRIVVDYEF"
     gene            complement(3869..4609)
                     /locus_tag="VFT03_08020"
     CDS             complement(3869..4609)
                     /locus_tag="VFT03_08020"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU4848130.1"
                     /translation="MGVRAEERGELHAVPAIAWAAAFVVCGIGDALLGRWVLLPLFVA
                     AFYGGMIVIDLITYPMLSFWRGWLRRRGLVAWYLVEVVGLWGGTTLILVLISSSWLGW
                     NWNEALPLQVLGGILLVASVTAGTWAVAKMGWARLLFAGALFPSGAGAEENGVPQRLV
                     VEGPYRYVRNPLYDTDFCLILGAALLTGSWALVLVAALYLAQLAIQLPLEERELRERF
                     GVPYLRYCELVPRFWPRRRPVRQRDLYT"
     gene            complement(4808..>5469)
                     /locus_tag="VFT03_08025"
     CDS             complement(4808..>5469)
                     /locus_tag="VFT03_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4848131.1"
                     /translation="AQIVVGIEAGELNVLIIGATGGTGRILLEQALEQGHEVTALARN
                     PAAVAPRDYRPRVLEGNALDPEAVEAAVEGQDAVLSALGTRSRKPTTLFSASTAYLVA
                     AMKKHGVRRLVCLTGIGAGDSKGHTGFLYDRVILPFVVNNLYEDKDRQEEIIRRSGLE
                     WVIVRPARLTNESATGEYRVFLSGDAYRATTISREDVAAFMLAQLSEDRYLHQTPVIT
                     Y"
BASE COUNT          987 a         1760 c         1712 g         1010 t
ORIGIN      
        1 gttatcgacc ggaatatcgg aggtatcgcg gctgatgcga ggacggcgcc gaccccgata
       61 ccatcctccg gatcgtcgtt gaagacccac gaggctcgcc cgtcggggtc gtcccgggcg
      121 tcgacgcgcc tgtggctccc gaagaccttg aactctccgg tcaggatgtt tacggccccg
      181 acgaagagct tggggcttgc gggcctgacc aggtcaccgt ctatgtcccc gaagggcacg
      241 ttgtcctcca ggatctctct cagccgctgc tgccagtagt cggagaaggc gttggggctg
      301 ctctcgaaga ggaagccggt cgtctgctgc cagcgcaacc acccgacgag ccagtcgttc
      361 agcagcttct ccggagcatt cctcgcggcg ttgtcctcga gccagaactt ctcgagcgaa
      421 cgcgcggcct ccgccgcggc cttcgatgcg ccctcctcgg cctggccatg ctcgagcagg
      481 gcgtaccagg ccagcagggc gcacacggcg cctcccgagg taccgctcaa ggccacgaac
      541 tcgtagcgct ggtcttgcag gagcgtcttg agcgccccgg ccgtgaaggc cgtatggctt
      601 ccaccgccct ggcaggctat ggcgattctt ttccttctat tgccctccaa tgccagcccc
      661 tttcggtggt cgtgttcacc ctcaaggcta acatcgcgcg ctgtgcgtag taagctaatg
      721 gggctttggt ttcttccgcg tcgcgcatct agaaagagat cttgcggatc ttcggcaagc
      781 gccggttcgt ggtgctggct ttcgccttgc tcgtcctctt ctggggatcg gccttcgccg
      841 tcgtcaaggt gggcctggac cactcgccgc cggtcctctt cgccggactg cgagccttga
      901 tcggcggggt ggcgatggtg atcgctgccc tcctgtgggg cgggaacccc aacctgcgcc
      961 gcgactggcc cgtgttcttg ctgttgagtc tcttcaacgt cgtgctgttt atcgggttcc
     1021 agacctacgc catcatgcac cttccctccg gctcggccgc ggtgctcgtc tacctgcaac
     1081 ctattctggt cggacttctg gcgtggccgc tccttggcga aacgctgacg gcgaccaagg
     1141 tcttcgggct cctgctcggg ttctcgggga tcgtcgcggt gagcgcgggg agcttctcgg
     1201 ggaccgcggc cgccctctcg cctctgggcg tggtccttgg ggcctcctct gcactcttct
     1261 gggctctcgg gaccgtgttt ttcaagaagc acgaggaacg ggtctccacc ctctgggcag
     1321 tagcgctgcc gttcctggtc ggcgggatgg tcctcacggc gttcgggctc tttgtagagt
     1381 cgtggagcga ggtttcgtta aacggcgtct tcgtcgccag ccttttgtat tcagggctcg
     1441 tggggatttc gctagcgtgg gtgatctggt tcgcgctcgt gcgcgccggc gaggcaagcc
     1501 gggtagcttc ctacatcttc gccgtaccgt tgaccgcggt tctgatcggg gtcgtcttcc
     1561 tcgacgagcc gctcggctac acgctcctga tcggcgccgc tttcgtcgta tccggcatct
     1621 acctcgtcaa ccgcgaaccg gtgaacaaga cgaaggagta gctaccctcg ccgaaacgca
     1681 gagggtgccg accgaagcgc ccggatgacg ggcaagcaca cccacggccg gctaaacggg
     1741 gtcagtacgt cacgacgttc aggcacacgg ccgggaagtc gtatccctcg ccggaatggt
     1801 acggtagagc cacttcgtgc acctgctccc ggatttcctc ctgtgcttcg gtagggaggg
     1861 accgcagtac gggtgcggcg gacgcagacg tctcggtgag gtagcgccag taggcctcct
     1921 gctcggcgaa cgaccagcgg aaagagacct cctcgacctc gggttcggcg aaactagctc
     1981 cgatgaggag ctcctggatg cgccccggat cggccattgc gaaggcgccg ggcgcaccag
     2041 gctcgggcgg gggaatatgt ccctgcgact ccagtaccct gcccaccagc gagagtgccg
     2101 ggttgcgctc gcggctggcc caaacggaga aagccagccg gcctcccggc ctgagcacgc
     2161 ggcgggtttc ggcgaaagcc gcagctgggt cggtcatcag catgtaggcc cagcggcaga
     2221 gaacaccgtc tacgctgtcc gtctcgaggt ccatccgctc ggcgtctagt agcctgaact
     2281 ccgcgttctt aacgccaagc tcttgcgctc gctggcgagc ggcagccacc atctcggggg
     2341 cgaaatccgt gatgaccacg cgtcctgact cacctaccag gcgcgccgcc atgagccctg
     2401 tgtcgcccag gccagcggcc aactcgagca cggtgtctcc cggttgcgga tcaagccttc
     2461 ggaccaacca ctcgctgatc gggcgagagg cctcccacag cttttcacgc tgagcgtacc
     2521 agccaggagc cacgtcattc caggtagctc tgctttgctg gcgggccgct tcgtgtttcg
     2581 ctgacatctc ctcgggcgat gtcatattcc tctcccttgt tcccttactc tcgtgtaaga
     2641 ctcgggcgcg cggccccgcg gcaggtagcg agaccgctct ccgtatactg aggataagga
     2701 gccggcaaga ccggcgcaag ctgatcgcat tccgattggt attgtttgca ctctaattac
     2761 ctggaaggca agtccgccat cgcgtctgtg cagtgcttgg cagagagctg gagaccgagc
     2821 tatggctcat ctaggtgtaa tccgaaggca caacgccatc aactgtggga aactgccgct
     2881 gcgggagggg cgagtggggg caaaacgaac atctgacttt agaagctgaa aggaggacaa
     2941 gcgcatgcag caaagccacg gaaatcagga agtcagacct ccggtgccac cattcaatga
     3001 ggagaccgcg aggcagaagg tcaaggcggc ccaggacgcc tggaataccc gcgaccccga
     3061 gagggtcgcc ctcgcctata cggaagactc caggtggcgc aaccgcgacg agtttttcga
     3121 gggccgggac gccataaaag agttcttgag gcgcaagtgg gccagggagc tcgactacag
     3181 gctagagaag aacctctggt gcttcaccgg caaccgcatc gcggtccgct tcgagtatga
     3241 gagtcacgac gcagaggggc agtggtggag atctcacgga aatgagttgt gggagttcga
     3301 cgagaacggc ctgatgcagc ggcgagatgc ctcgatcaac gactacaaga tagacgagtc
     3361 cgagcgcaag ttccgctgga ccaggtgaga caccacgata ggcctcatga gacgatagag
     3421 gagcccgaac gccactgaga tcggcctgag aaggagaacg catgatcgac cacctcgtgt
     3481 ttctggccgc gaacgaggac gcctcgcccg aggacatcga ggacctgatc tcctcgatcc
     3541 ggggcctgaa ggacaccgtt ccgggggtcg tcgatctctc ggtaggggag aacttcagcc
     3601 cgcgctccgg cggctacacc cacggcctat tcgtccgctt cgagacggca gacaacctgc
     3661 aagggtacct ggagcacccg gaccaccagg ccgtcgccga gaagctcgac aggctgacca
     3721 gccggatagt cgttgactac gaattctagg acgatggatc ggacaccaca ccgcgagtgc
     3781 atcacgaccg gcgagctcga aactaagctc gatgccggag aagggcccga gctgctcgac
     3841 gtgctcgctc cgcagcggcg cggctggctc aggtgtaaag gtctcgttgc cggacgggcc
     3901 tacgcctcgg ccagaagcgg ggtacgagct cgcagtatcg gagatatggc acgccgaagc
     3961 gctccctgag ctcgcgttct tccaggggga gctggatggc gagctgggcg aggtagagcg
     4021 cggcgaccaa cacgagggcc cagctaccgg tgagcagcgc cgcgcccagg atcaggcaga
     4081 agtccgtatc gtagagtggg ttgcgcacgt accgatacgg cccctctacc accaggcgtt
     4141 gcggaacgcc gttctcttcg gcccctgccc ccgacgggaa cagggctccg gcgaacagca
     4201 gcctggccca tcccatcttc gccaccgccc acgtccctgc cgtcaccgag gctacgagga
     4261 gtatgccgcc gaggacctga agcggtaacg cctcattcca gttccatccc aaccacgagg
     4321 aggagatcag gaccaggatg agcgtcgtcc cgccccagag cccgacgacc tcgaccagat
     4381 accatgctac gagcccccgt ctcctgagcc agcctcgcca gaaggagagc atagggtagg
     4441 tgatcagatc tatgactatc atgccgccgt agaacgcggc gacgaagagc ggtagcaaga
     4501 cccatcgccc gagcaaggcg tccccgatac cgcacacgac gaacgccgcc gcccaggcta
     4561 ttgcgggcac cgcgtgaagc tcaccgcgtt cctctgcccg aacgcccatg acgtgaattc
     4621 tatagcgatt cggctcaggg ggtagaacgc cgaggttccc atgatcgcct cggggcagaa
     4681 cgtgaagcgg ctgccggagt tcggatgccg gaacccagga aggcttcgca cgccgtagcg
     4741 ctgcactcgc tgtaggtgcc tgcgctacat gcgccgccat cgcgcgacgc gttgcaatga
     4801 gtcttcctca gtaagtaatc acaggcgtct gatgcaggta gcgatcctcg ctcagttgag
     4861 cgagcatgaa agccgcgacg tcctctcggg atatagttgt ggctctgtaa gcatccccgc
     4921 tgagaaagac ccggtactca cccgtggccg attcgttggt gagccgggcc gggcgcacga
     4981 tcacccactc cagaccgctt cgtctgatta tctcctcttg acggtctttg tcctcgtaca
     5041 ggttattcac tacgaagggc aagatgaccc tgtcgtagag gaaaccggtg tgccccttgc
     5101 tgtcgccagc cccgataccg gtgaggcaaa ccagccggcg cacgccgtgc ttcttcatcg
     5161 cggcgacgag gtatgcggta ctggccgaga aaagggtcgt gggcttcctg ctgcgcgtac
     5221 ccagagccga gagcaccgcg tcctgtccct ccacggcagc ttcgaccgcc tccggatcca
     5281 gcgcgttccc ctccagtacg cgaggacggt agtctcgggg tgcgacggca gcggggttgc
     5341 gagccagggc cgtcacttcg tggccctgtt ccagcgcctg ttccaacaag atccgccccg
     5401 tgccccccgt cgcgccgatg attagaacgt tcaattctcc tgcctctatt cctaccacta
     5461 tttgcgccg
//
LOCUS       DASTUV010000396         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_617220, whole genome shotgun sequence.
ACCESSION   DASTUV010000396 DASTUV010000000
VERSION     DASTUV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617220"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VFT03_08030"
     CDS             complement(<1..1179)
                     /locus_tag="VFT03_08030"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEU4848132.1"
                     /translation="MPTLLARNADILVTMDEGRRELSDGGLFVRDGIIEQVGPTAELP
                     RDADVVLDMRGQILLPGFVNTHHHLDQTLTRALPAVQDINLFPWLRVHYPIWARRTPE
                     ASRWSTLVGLAELAASGCTTVFDHTYVFPNGCRIDDQISAAQEIGVRFHASRGSMSLG
                     ESWGGLPPDSCVEEEAAILDDSRRVIETYHDASRGSMTQVVLAPCSPFSVTPELLMET
                     AELARSYGVRLHTHLCETFDEELFTLSNFEQRPVAWMETMGWLGDDVWFAHAVHVDDD
                     EIREFARTGVGVAHCPCSNMRLASGIAPVKHYLAAGVRVGLGVDGSASNDASNIMLEA
                     RQAMLLARLRIGLLPPEGPQTLLSTSDPLRGSEWMTAREALEIATRGGAAVLGRSDIG
                     A"
     gene            1371..2162
                     /locus_tag="VFT03_08035"
     CDS             1371..2162
                     /locus_tag="VFT03_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848133.1"
                     /translation="MVQVLLQVPTALLGFLIILLAVSLAVAGTALVHNKVPVKLRESH
                     TVPLGLLNVGLTGMFGIIIGLTAFLVLGEYSDAQQALQSEAGDVEEIYRLAGPLPEPK
                     QEQIQGLATSYAHVVVNEEWPLMEEDRTSPRADALIEDLRSTIQEGYRTSTGAEQDFF
                     GEELDVMDELDADREARVLAVHQRLPSILWIALVILAIATLGFSSLAGMENRRLHLLT
                     VGILAAGITVAMFTIGVLDRPYGTDFRVQPQPFELLLNEIEGNSR"
     gene            2396..3700
                     /gene="codA"
                     /locus_tag="VFT03_08040"
     CDS             2396..3700
                     /gene="codA"
                     /locus_tag="VFT03_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HEU4848134.1"
                     /translation="MYQLIVRGARLGDEVVDVGIEGGRIARIAARLDEEAGREIDAGG
                     RLVSPPFIESHVHLDTTLTAGEPRWNESGTLFEGIQIWSERKKSLTREDVISRATQLL
                     RWQAAQGVLHVRTHADVTDPEFTGLKALLEVKEKVRGWMDVQIVAFPQEGMISFPNGV
                     ELMEEALRLGADAVGGIPHYEQTREMGVESVKESFRLAGKHDRSIDIHCDETDDPQSR
                     FLEVMAAEAIRTGMGPRVTASHTTAFGSYDNAYAFKLMGFLAKSGMNFVANPLVNITL
                     QGRYDTYPKRRGITRVKELWQNGLNVSLGYDDIMDPWYPLGTGNMLQPAHMAVHACHM
                     TGREEVTACFDMITVNAARTLGLKDYGIAEGKPGNLVLVDAPDRWEAIRRLAPTTLVV
                     KDGEVISETSPPESRLMGEVVDFERKATRSSGEEKGGQATWR"
     gene            3678..>4252
                     /locus_tag="VFT03_08045"
     CDS             3678..>4252
                     /locus_tag="VFT03_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEU4848135.1"
                     /translation="MDRRPGVEDRVGHERAELEEAVHIEKHNSIAPVPEEYKDATPLD
                     QFWIWAGANIAPINWVLGALGIVLGLGFWDTVLVLALGNVVGVLIFGAFVLMGQRTGV
                     TQMVLSRSAFGRRGAYFPAFFQIVIATGWIAINTWIILDLSVALLEKLGIPGTPTTKV
                     GVVLVLMAIQDGLATLGFYAIRTFEKWTVPIT"
BASE COUNT          833 a         1294 c         1381 g          744 t
ORIGIN      
        1 cgcgccgatg tccgagcggc ccaggacggc tgcgcctccg cgggtcgcta tctccagtgc
       61 ctcgcgcgcc gtcatccact cgctaccgcg gagcggatcg gaggtcgaga gcagggtctg
      121 cggaccctca ggcgggagca gaccgatgcg gagccgggcc agtaacatcg cctggcgcgc
      181 ttcgagcatg atgttcgacg cgtcgttgct cgcggaccca tcgaccccca gcccgacgcg
      241 cacgccggct gcgaggtagt gcttgacggg tgctatgccg gaggcgagcc gcatgttcga
      301 gcaggggcag tgcgcgaccc ccacaccggt cctggcgaac tcacggatct cgtcgtcgtc
      361 gacgtgtacg gcgtgggcga accagacgtc gtcgccaagc cagcccatcg tctccatcca
      421 cgctaccggc cgctgctcga agttcgagag cgtgaagagt tcctcgtcga aggtctcgca
      481 gaggtgggtg tgcaggcgca ctccgtagga gcgggcgagc tcggccgtct ccatcagcaa
      541 ttcgggagtg accgagaaag gagagcacgg cgccagcacg acctgggtca tcgagccgcg
      601 cgaggcgtcg tggtaagtct cgatgacccg cctggagtcg tcgaggatcg ccgcttcctc
      661 ctcgacacag ctatccggcg gcaacccgcc ccaggactcg cccagggaca tggagccgcg
      721 agaggcgtgg aaccgcacgc cgatctcctg ggccgccgag atctggtcgt cgatccggca
      781 gccgttcggg aagacgtagg tgtgatcgaa gacggtagtg cagccggacg cggcgagctc
      841 ggcgaggcca acgagggtgc tccagcgaga cgcctccgga gtccggcggg cccagatcgg
      901 gtagtggacc cggagccagg ggaacaggtt gatgtcctgg actgccggga gcgcgcgggt
      961 cagcgtctgg tcgaggtggt ggtgggtgtt cacgaagccg ggcaacaaga tctggcccct
     1021 catgtccagc acgacgtcgg cgtcccgggg cagctccgcg gtagggccga cctgctctat
     1081 gatcccatcc cggacgaaca gccctccatc tgagagctcg cggcgccctt cgtccatcgt
     1141 gaccaggata tcggcgttcc gcgccagtag cgtaggcaag gtcagatcct cctaacaagc
     1201 tcgaagtcca gtcgtcaaac cacgtgacgc tcgaattttc agacagtcct cacgtttcgg
     1261 acagtccctc aacgatatcc ggcgaacaga acctcaagga tgcgataagc tgcgccaagc
     1321 gtcaatctac cttcacacag attgctgaca agatagggga aggcagataa ttggtacaag
     1381 tattgctaca agtacccacg gcgctgttgg gcttcctgat cattttgctg gccgtgtcac
     1441 tcgcggtagc ggggacagcc ctcgtacaca ataaggtgcc agtcaagctg cgcgaatcgc
     1501 acactgtccc cctcggcttg ctcaacgtcg gacttaccgg tatgttcggc atcataatcg
     1561 gccttacggc ttttctcgtg ttgggcgagt acagtgacgc ccagcaggcg ctgcaaagcg
     1621 aagccggaga cgtagaggag atttacaggc ttgccggacc gcttcccgaa ccgaagcaag
     1681 aacaaatcca agggttagcc acctcttatg ctcatgtggt cgtaaacgag gaatggcctc
     1741 ttatggaaga agacaggacg agtccacgcg ccgacgccct cattgaggac cttcgcagta
     1801 ccatccagga agggtacagg accagcactg gtgccgaaca agactttttt ggggaggaac
     1861 tcgatgtgat ggacgagctg gacgcagaca gggaggctcg cgtgctcgcc gtacaccagc
     1921 gcttgccttc tatcctctgg atcgctctag taatcctagc gatagccaca ctaggattct
     1981 cttccctggc cggtatggag aaccgccgac tacacctgct gaccgttggt atcttagctg
     2041 cgggaatcac cgtagctatg ttcacgatcg gcgtcctcga tcgcccctac ggcacggatt
     2101 ttagggtgca gccgcagccg ttcgaattgc tgctgaacga gatcgaaggg aacagcaggt
     2161 aataagttcc tagcaagttc tggaagcctc ctcgtgtagc ccagcgcgag ctgcacgtca
     2221 gtagtttctt ctgtcagcag ggcgtggaac ctaagctcgg acggttttgg gttcgtgagc
     2281 cttgatgctc atcgagtacg agctcatgag gatcgcgacc tcctatattg actaccttcg
     2341 cggcgaaaag tagtctggcg gcgatggccg ggaaggcgtc tgagggaggt cgtgcgtgta
     2401 ccagttgatc gtacgcgggg cgcggctcgg ggacgaggtc gtcgacgtcg gaatcgaggg
     2461 cggcaggata gcccggatcg ccgcgcggct cgacgaggag gccgggcgcg agatcgacgc
     2521 cgggggccgg ctcgtctcgc cgcccttcat cgagtcccac gtgcaccttg acaccactct
     2581 gaccgcaggg gagccccggt ggaacgagtc cgggacgctc ttcgaaggaa tccagatctg
     2641 gtccgagcgc aagaagagcc tcacccgcga ggacgtgatc tcgcgcgcta cacaactcct
     2701 tcgctggcag gcggcccagg gcgtgctgca cgtccgcacc cacgccgacg tcacagaccc
     2761 tgagttcacc ggcctcaaag ccctgctcga ggtcaaagag aaggtgcgcg gctggatgga
     2821 cgtccagatc gtcgccttcc cgcaggaggg catgatctcc ttcccgaacg gcgtagagct
     2881 gatggaggag gcgctgaggc tcggtgccga tgcggtcggg ggcatacctc actacgagca
     2941 gacccgcgag atgggggtgg agtccgtgaa ggagagcttc cgcctcgccg ggaagcacga
     3001 caggtccata gacatccact gcgacgagac cgacgacccg caatccaggt tcctcgaagt
     3061 catggcagcc gaggccatac ggaccgggat ggggccgcgc gtcaccgcga gccacaccac
     3121 tgcatttgga agctacgaca acgcctacgc cttcaagctg atggggttcc tggcgaagag
     3181 cggtatgaac ttcgtggcga atcccctggt caacataacg ttgcaggggc gctacgacac
     3241 ttatcccaag cggcgcggga tcacgcgggt taaggagctc tggcagaacg ggctcaacgt
     3301 ctccctgggc tacgacgaca tcatggaccc gtggtatccc cttggcacgg gcaacatgct
     3361 gcagccagcc cacatggccg tccacgcgtg ccacatgacg ggccgcgagg aggtaacggc
     3421 ctgcttcgac atgattaccg tgaacgccgc ccgcacgctg ggactaaaag actatggaat
     3481 tgccgagggc aaacctggca atctggtgct ggtggacgcg cctgacagat gggaggcgat
     3541 ccgacgcctt gcgcctacga cgcttgtggt caaggatgga gaggtgatct ccgagacgag
     3601 cccgcccgag tcccgtctca tgggcgaggt cgtggatttc gagaggaaag cgacgcggag
     3661 tagtggagag gagaaaggtg gacaggcgac ctggcgttga ggatcgggtg gggcacgagc
     3721 gtgccgagct cgaagaagcc gtacacatcg agaagcacaa cagcatcgcg cccgtccctg
     3781 aggagtacaa ggacgcgacg ccgctggacc agttctggat ctgggccggc gctaacatcg
     3841 ccccgatcaa ctgggtgctt ggcgcgctcg ggatcgttct cggcctgggg ttctgggaca
     3901 ccgtgctcgt cctcgcgctc gggaacgtcg tcggggtact gatcttcggc gcgttcgtgc
     3961 tgatgggcca gaggaccggg gtgacgcaga tggtcctctc gcgcagcgcg ttcgggcgca
     4021 gaggcgcgta cttccccgcc ttcttccaga tagtgattgc gaccggctgg atcgcgatca
     4081 acacctggat catcctggac ctcagcgttg ccctgctcga gaagctcggc atccccggca
     4141 cgccgacaac caaggtcggc gtggtgctcg tcctgatggc gatacaggac ggcctcgcca
     4201 ctttaggctt ctacgccatc cgtaccttcg agaagtggac cgtgcccatc ac
//
LOCUS       DASTUV010000397         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1439690, whole genome shotgun sequence.
ACCESSION   DASTUV010000397 DASTUV010000000
VERSION     DASTUV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439690"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFT03_08050"
     CDS             <1..681
                     /locus_tag="VFT03_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEU4848136.1"
                     /translation="AIFMAPPAPFVPQHLQGKPVLGMVAMYIGAADEGQNVVTPLKEL
                     GPPAVDMIQQMPYTAFQAILDPTAPWGLLNYNRGEHLTGLSDGAIDTYVEHASEIARV
                     SPWTQTIIFRHGGAVSRIPEDANAASHRDDAYIAHPIACWEDPADSDRHIAWIRGFSE
                     AIRPYTTGGVYLNFEPDVGEERVRAGYGADKYARLVALKDKWDPENLFRVNQNIKPSH
                     ETYVTAPA"
     gene            1101..1595
                     /locus_tag="VFT03_08055"
     CDS             1101..1595
                     /locus_tag="VFT03_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848137.1"
                     /translation="MASFVQTAITVVIVGLFWATGQDPYLGLYVLMAILGSFAILIVQ
                     TLCSFAVIGYFARNHPEARHWFRTFLAPLIGGIGMAAVVVLLILNLGTATGDAAETLF
                     FKLIPWTVVGVFLGGIVLALYLRARRPEQYAIIGRTIYEDTAERPDVDLDGRGEHAVN
                     PLKS"
     gene            1654..>3345
                     /locus_tag="VFT03_08060"
     CDS             1654..>3345
                     /locus_tag="VFT03_08060"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEU4848138.1"
                     /translation="MIERPAAVADHPLAPLTLEEIQAAVRIVREERGLGPKHRFAGLT
                     LNEPPKEQVLSYQPGDTFEREALAVILDGNEGTTYEAVVSLSQGTVASWTHIPDIQPP
                     IVLEEFDECEAACKESPEFQEALRKRGVEDVDRVLVDPWSAGSYGDEEGRRLSRALTW
                     VYVEGDHNAYAHPVDNLVTVVDLNTLEVVRVEDYGVVPIPQESGAYVPDGRPMRPDLK
                     PLEITQLEGPSFEIDGHEIRWQKWSFRIGFTPREGLVLYTVGYEDGGKVRPVLYRASL
                     SEMVVPYGDPRPGQWRKNAFDAGEYNVGALANSLELGCDCLGEIRYFDAVMVNGAGDP
                     VVIKNAVCLHEEDAGLGWKHYDMRADTAEVRRSRRLVISFIATVANYEYGFYWNLYQD
                     GTIEFDGRLTGILSTGAVGPDVKPKHGQLLNREGLYAPIHQHYMNFRLDLDVDGRNNK
                     IYEVHTEAVPPGDENPHSNAFFSKSTLIERESEAQHTTDSMSARHWRIESPETTNKVG
                     EPCAYRLIPQSNILPFFQPDSPIAKRAGFATKHFWATQFSPDERHAAGAYPNQNPG"
BASE COUNT          651 a         1096 c         1056 g          542 t
ORIGIN      
        1 gcgatcttca tggcgcctcc cgcaccgttt gtgccgcaac atctgcaagg caagcctgtc
       61 ctgggcatgg tcgcgatgta catcggcgcc gcggatgagg gacagaacgt tgtgacgccg
      121 ctcaaagagc tcgggccacc cgcggtagac atgatccagc agatgcccta caccgccttt
      181 caggcgatcc tcgacccgac tgctccatgg gggttgctga attacaaccg gggcgagcat
      241 cttacgggac tctctgacgg agccatcgac acctatgtgg agcatgcgag cgagatcgcg
      301 cgtgtctcac cgtggaccca gacgatcatc ttccgccacg gcggtgcggt cagccggatc
      361 ccggaggatg ctaacgccgc tagccatcgc gatgatgcgt acatcgctca cccgattgcc
      421 tgttgggagg atccggcgga ctcggaccgg cacatcgcct ggatcagggg attctcagag
      481 gcgatacgcc cttacacaac cgggggagtg tatctaaact tcgagcccga cgtgggggag
      541 gagcgggtgc gtgcgggata tggcgcggat aagtacgcga ggcttgtagc actgaaggac
      601 aagtgggacc cagagaacct cttccgggtc aaccagaaca tcaaaccgag ccacgagacg
      661 tatgtgacag ccccagccta agccaagtgg aagcgcacct tgacggtcga ggagggccga
      721 gcgtgggtgc gttgcgactc ggcacacgag gagatgctga caccactttt gacaccactg
      781 agagcgctac acccagaaac gtcgggcaac caccagcaaa gaaatcggct tatgtaagcc
      841 tattctgcaa catccagcaa cacctggaaa cgggagtcgg cggattgtgg ctccgaaggt
      901 cgcgggttcg agccccgtcg gtcacctcct cctttgcagg taaaacgcga ccctgattat
      961 tgtcaccgag agcggtgaca gcggtagggc agcaataggc cttccacaac tgcgcctcgg
     1021 gctacctcta cgccatcggc cgcgagggct ttatcagccg gagcctcggc aagacgcacc
     1081 ccacgcacgg ttcgccctac gtggcgtcgt tcgtccagac cgctatcacc gtggtgatag
     1141 taggcctctt ctgggccacc ggccaggatc cttacctcgg actctacgtg ttgatggcga
     1201 tcctgggaag cttcgcgatc ctcatcgtgc agaccctgtg ctccttcgcg gtcatcggct
     1261 acttcgcccg caaccacccc gaggcacgcc actggttcag gaccttcctt gcgccgctga
     1321 tcgggggaat aggcatggcc gccgtcgtag tgctccttat cctcaacttg ggcaccgcca
     1381 ccggagacgc ggccgagacg ctgttcttca agctgatacc gtggaccgtg gtgggcgtct
     1441 tcctcggtgg aatcgtgctc gcgctctacc tgcgcgccag gcgccccgag cagtacgcga
     1501 tcatcggccg gaccatctac gaggacaccg cggagcgacc cgacgtcgac ctcgacggcc
     1561 gcggggaaca cgcggtgaac cccttgaagt cataataaac tacgcgtgtt cggtcggagg
     1621 accgagatcg ggaaggagga ggaattcatg gctatgatcg agagaccggc cgcggtggcc
     1681 gaccatcccc tggcgccgct tacgctcgag gagatacagg ccgccgtgcg catcgtgcgc
     1741 gaggaacgcg ggcttgggcc gaagcaccgc ttcgccgggc tcaccctcaa cgagccgccc
     1801 aaggagcagg tgctctcgta tcaaccgggc gacaccttcg agcgcgaggc cttagcggtg
     1861 atcctcgacg ggaacgaggg gacgacctat gaggcggtgg tctcgctctc ccagggcacg
     1921 gtcgcctcgt ggacccacat ccccgacatc cagccgccta tagtgctcga ggagttcgac
     1981 gagtgcgaag cggcttgcaa ggagagcccg gagttccagg aggccttacg caagcgcggc
     2041 gtcgaggacg tggaccgcgt gctcgtggac ccgtggtcgg cggggagcta cggcgacgag
     2101 gaggggcggc gtctgagccg tgcgctcacc tgggtctacg tcgagggcga ccacaacgcc
     2161 tacgcccacc cggtggacaa cctcgttacg gtcgtcgacc tcaatacgct ggaggtggtg
     2221 cgggtcgagg actacggcgt ggtgccaatc ccgcaggagt ccggggccta cgtcccggat
     2281 ggacggccca tgcgccccga tctcaagccg ctggagatca cacagctcga gggcccgagc
     2341 ttcgagatag acgggcacga gatccgttgg cagaagtgga gcttccgtat cgggttcacc
     2401 cctcgcgagg ggctcgtcct ctacacggtc ggctacgagg acggggggaa ggtgcgaccc
     2461 gtcctctacc gggcctccct ctcggagatg gtcgttccct acggcgaccc acggccgggc
     2521 cagtggcgca agaacgcctt cgacgccggc gagtacaacg tcggcgccct ggccaactcg
     2581 ctcgagctcg ggtgcgactg cctcggcgag atccgctact tcgacgccgt gatggtcaac
     2641 ggcgccggag accccgtcgt cataaagaac gccgtctgcc tgcacgagga ggacgccggc
     2701 ctcggctgga agcactacga catgcgggcc gacacggcgg aggtgcgccg ctccaggcgt
     2761 ctcgtcatct cgtttatcgc caccgtggcg aattacgagt acggcttcta ctggaacctc
     2821 taccaggatg gcaccataga gttcgacggc aggctcaccg gcatcctctc caccggcgcg
     2881 gtcggaccgg acgtgaaacc caagcacggt cagctactta accgcgaggg gctctacgcc
     2941 ccgatccacc agcactacat gaacttccgg ctcgacctcg acgtcgacgg ccggaacaac
     3001 aagatctacg aggtgcacac cgaggcggtg ccaccgggcg acgagaaccc gcacagcaac
     3061 gccttcttct cgaaatcgac cttgatcgag cgcgagtcgg aggcccagca caccaccgac
     3121 tccatgtcgg cgcgccactg gcggatcgag agcccggaga cgacgaacaa ggtcggcgag
     3181 ccctgcgcgt accggctgat cccgcagagc aacatcctgc cgttcttcca gcccgacagc
     3241 ccgatcgcga agcgcgccgg gttcgccacc aagcacttct gggcgaccca attctctccg
     3301 gacgagcgcc acgcggcagg tgcctacccg aaccagaacc cgggc
//
LOCUS       DASTUV010000398         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_506463, whole genome shotgun sequence.
ACCESSION   DASTUV010000398 DASTUV010000000
VERSION     DASTUV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506463"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1096
                     /gene="rfbD"
                     /locus_tag="VFT03_08065"
     CDS             230..1096
                     /gene="rfbD"
                     /locus_tag="VFT03_08065"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU4848139.1"
                     /translation="MERGAAEVRVLISGAGGQLGLELAEILPGRGHETVALPRAELDI
                     ADPDAVEDAVKGYAPDLVVNAAAYTNVDGCETETDLAYAVNALGPRNLAQTCEGLGCK
                     LLHVSTNYVFDGEADRPYEPFDLPNPISAYGRTKLAGEEYVKHLTRRWYVVRSAGVYG
                     RGHNFVRTMLRVAGERDVLKVKADEFISPTYARDLAKGIARVAEAGRYGLYHLTNAGS
                     CSWYEFAREIFRLAEVEAEVVSIPGSEYPLPAARPTNGVLSSLGTFELRHWREALADY
                     LGREAVLSGARG"
     gene            1104..2255
                     /locus_tag="VFT03_08070"
     CDS             1104..2255
                     /locus_tag="VFT03_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU4848140.1"
                     /translation="MTIFGRRRVYGALREAKRSLEAAMGTFRSKTSKGARRQIAIKDA
                     EIVALRRELTRARGEEPSPGAASTASFFVVGYQKSGTTWLMQMLDAHPEILCKGEGRF
                     FGGGWRQESVKRIDARRPPSSLYNAVLDAEYLRLWIERSVWSRNDDTEEHLTNLTRMA
                     IHYFLNGELSKTNKRLVGDKSPLLTPETMEEIARIYPEARTIHIIRDGRDAAVSAAHH
                     SWNFGKALKKSYEVSAKRESTRRDPRELKEAGESIFAEDQLRKIAAEWAARVGRAMED
                     GPVLLGDHYREVQYEGLSVRPEESVRRLLEFLGAEAGDETVRRCVESASFERLSRGRQ
                     RGEEDPSSFFRKGIAGDWRNVFTEEDKRVFKEEAGGLLIKLGYEESDQW"
BASE COUNT          462 a          694 c          812 g          370 t
ORIGIN      
        1 gaactgcaat gtgaggagca caaacgctgc cgcgagccta gacgtcaagt gacccttaaa
       61 agggtgtagc acagggctta acaaccctga gacatcaggt acggaccggt gctcgttctg
      121 agccccggtc tttttgttgc cttgccggcg tccgccgatg tggctccggg gttttggtag
      181 agtatcggtg cctgagatcg agagttctgg agcgtgaggt acatgatcag tggagcgggg
      241 tgctgctgag gtgagggtgc tgattagcgg ggcgggcggc cagctcgggc tggagctggc
      301 cgagatcctg cccgggcgcg gccacgagac cgtggcgctc ccccgcgcgg agctcgacat
      361 tgcagatccc gatgctgtgg aggatgccgt caagggctac gcgccggacc tcgtcgtaaa
      421 cgcggccgcc tacaccaacg tggacggctg cgagacggag acggacctcg cttacgcggt
      481 caacgcgctc gggccccgca acctggccca gacgtgtgag gggctggggt gcaagctcct
      541 gcacgtgagc accaactacg tcttcgacgg ggaggcggat cgaccctacg agccctttga
      601 cctgccgaac ccgatcagcg cctacggccg caccaaactg gccggggagg aatacgtgaa
      661 acatttgaca cggcgctggt acgtcgtccg ttctgccggc gtctacgggc gggggcacaa
      721 cttcgtacga accatgctgc gtgtagccgg ggagagggat gtgctgaagg tcaaagccga
      781 cgagttcatc tcgccgacct acgcccgcga cctcgcgaag ggcatagcca gggtcgcgga
      841 ggccgggcgc tacggcctct accacctcac caacgctggc tcctgctcct ggtacgagtt
      901 cgcgcgcgag atcttccgtc tggccgaggt cgaggctgag gtggtctcca tccccggctc
      961 cgagtacccg ttgcctgccg cgcggccgac caatggcgtt ctctcttctc tggggacctt
     1021 cgagctccgc cactggcgcg aggcgctcgc agattacctt gggcgggagg ccgtgctgag
     1081 cggcgcccgc ggctgagggg caggtgacga tcttcggacg ccgcagggtc tacggcgcac
     1141 tgcgtgaggc gaagcggtcc ctggaggccg cgatgggcac ctttcgatcg aagacgagca
     1201 agggggcccg caggcagata gccatcaagg acgccgagat cgtggcgctc aggagggagc
     1261 tgacccgggc gcggggagag gagccctcac ctggtgccgc gagcacggcg tccttcttcg
     1321 tcgtgggcta ccagaagtcc gggacgacct ggttgatgca gatgctcgac gcccacccag
     1381 agatactgtg caagggcgag ggtcgcttct tcggcggggg ctggaggcag gagtccgtca
     1441 agcggatcga cgccaggcgg ccgcccagct cgctctacaa cgcggtgctc gacgccgagt
     1501 acctgaggct ctggatagaa aggtcggtct ggagcaggaa cgacgacaca gaggagcacc
     1561 tgaccaacct cacccgcatg gccatccact acttcctcaa cggcgagctc tccaagacta
     1621 acaagcggct ggtaggcgac aagtcgccgt tgctcacccc ggagaccatg gaggagatcg
     1681 ccaggatcta ccctgaggcc aggaccatcc acatcatccg cgacggaagg gacgcggcgg
     1741 tctccgcggc acaccactcc tggaacttcg gcaaggcact aaaaaaaagt tacgaagtat
     1801 cggcgaagcg cgagtccact cgcagggatc ctcgggagct caaggaggcg ggcgagagca
     1861 tattcgcaga agaccagctc aggaaaatcg ccgcagagtg ggccgcaagg gtgggcaggg
     1921 ccatggagga cgggccggtt ctgctcggag accattaccg tgaggtgcaa tacgagggcc
     1981 tctcagtgag gccggaggag agcgtacggc ggctgctgga gttcctcgga gccgaggcgg
     2041 gcgacgaaac cgtgaggcgg tgcgttgagt cggccagctt cgagaggctc tcaaggggca
     2101 gacagagggg cgaggaggat ccctcctcct tcttcaggaa gggtatagcg ggcgactggc
     2161 gaaacgtctt caccgaggag gacaagcggg tcttcaaaga ggaagcgggg ggtctgctaa
     2221 tcaagctcgg ctacgaagag agcgatcagt ggtaggaggt gctgctgcca gccttcttcg
     2281 aacaacttac cacacccaac ggcaaccaca acgcgggaga cctgcacttt ggcaagga
//
LOCUS       DASTUV010000399         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1726376, whole genome shotgun sequence.
ACCESSION   DASTUV010000399 DASTUV010000000
VERSION     DASTUV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726376"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..686
                     /locus_tag="VFT03_08075"
     CDS             303..686
                     /locus_tag="VFT03_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848141.1"
                     /translation="MGVGPDCGPRSDVVGVEAARRLARGGFEVRVIEHESGHYEVREV
                     PYGKVYDWRPDRVVFECDCGETNVSFGSAIVCSCGTVHNDVSGEGQQEVSPHPWLEDY
                     EEWRRERDARDLRHEYFVFVEESDA"
     gene            679..1071
                     /locus_tag="VFT03_08080"
     CDS             679..1071
                     /locus_tag="VFT03_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848142.1"
                     /translation="MLNATETSLLEAGPREGPSRSWYLIARREGNRLEVLTLAGQPRR
                     EILPLFTTTGSARDFLRRGGVGVDWWVRESTAGELVSILVGYLPHVDRVVLDPVFGVS
                     TGDAEPQSPSKKEFVSVLLGEPLVAPAR"
     gene            1138..1530
                     /locus_tag="VFT03_08085"
     CDS             1138..1530
                     /locus_tag="VFT03_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU4848143.1"
                     /translation="MRRENVTSGTVWEREVGYSRAVRRGPFVYVSGTTATDGSGGFVG
                     VGDPYAQTIQTLNNIKVALQAAGAGLSDVVRTRIYVVNIDDWEKIGAAHGEFFGKIRP
                     ATSMVEVRRLIAPEVLVEIEADAFLDLG"
     gene            complement(1551..>2124)
                     /locus_tag="VFT03_08090"
     CDS             complement(1551..>2124)
                     /locus_tag="VFT03_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848144.1"
                     /translation="LAAVLEEISTALRDGDREAVERALARARTIDDDRVTNFYEALAA
                     GYETAQLSPTRRRALEHLELYASAGTRIDLAVINTRVLARGAANALRRGDTIPPPLPE
                     AVQDLARAVRALATYLEETSGPEETRRFALEAARKATEILKERHDLAISVLVGQVRSA
                     AVDLLRSTGMDQASALQALEETAGRASEIG"
BASE COUNT          320 a          652 c          761 g          391 t
ORIGIN      
        1 actcgccctc cggcgtgtgc gttgagatca tgtcggtcga atgttcggcc aggagccggt
       61 agagctcgtc gctctcttgc agcctctgtt ttacccccca ccgccgggtt cccggcgccc
      121 ccggttctcc ggtcaggctc acgttggtgt cctccgcgtt gcaaccagtg gcaatatatt
      181 agcctattcg tctaccctcg acgcgtgagg aagaaccggg gaccagcttc gggggaggtc
      241 tgggtaattt gacccaggag tgtccaggag atgtttccgt ctatcgatgt gagagataca
      301 gtatgggggt tggacccgac tgcggtcctc gttcggacgt ggtcggcgtc gaagcggctc
      361 gccggctggc gagaggaggt tttgaggtgc gggtgatcga gcacgagagc gggcactacg
      421 aggtccgcga ggtgccctac ggcaaggtgt acgactggcg tccggatcgc gtcgtcttcg
      481 agtgcgactg cggagagact aacgtctcgt tcgggtcagc gatcgtgtgc tcgtgcggga
      541 cagtccacaa cgatgtctcg ggagaaggac agcaggaggt gtccccccat ccctggctcg
      601 aggactacga ggagtggcgc agggagaggg atgccagaga cctgcggcac gagtacttcg
      661 tcttcgtgga ggagagcgat gcttaacgcg acggagacgt cgctgctaga ggcggggccc
      721 cgcgaaggtc cttcgcggtc gtggtacctc atcgcgcgca gggaggggaa ccggttggag
      781 gtgttgaccc ttgccgggca accccgcaga gagatcctcc ccctcttcac aaccaccgga
      841 tcggcccggg acttcctccg tcgcggcggt gtcggcgtgg actggtgggt gagggagtct
      901 accgcaggcg agctggtctc gatcctggta ggctacctcc cgcacgtgga ccgggtcgtc
      961 ctcgaccccg tattcggggt atccaccgga gacgcggagc cacagagccc tagcaagaag
     1021 gagttcgtct ccgtccttct cggcgagccg ctcgtggccc ccgcgcgcta agccaagcat
     1081 cccgctctat ccaggctaga atgtgccgca gcgtttccct gtgtgcgggg gctttgcatg
     1141 aggcgagaga acgtgacgag cggcacggtg tgggagcggg aggtggggta ctcgcgggcg
     1201 gttcgtcgcg ggcccttcgt gtacgtctcg ggtactaccg caaccgatgg tagcggcggc
     1261 ttcgtggggg tgggggaccc ctacgcgcag acgatccaga cgctcaacaa cataaaggtg
     1321 gcgttgcagg cggcgggggc cggactctct gacgtggtgc ggaccaggat ctacgtggtc
     1381 aacatagacg actgggagaa gataggggcc gctcacggcg agttctttgg gaagatacga
     1441 cccgccacca gcatggtcga ggtccggcgc ctcatcgcgc ccgaggtgct cgtcgagatc
     1501 gaagccgacg ccttcctgga cctcgggtag gggctgttcg cgaacggctc ctacccgatc
     1561 tcggatgcgc ggcccgcggt ctcctccagc gcctgaaggg ccgaggcctg gtccatcccc
     1621 gtgctcctga ggaggtccac cgcggcagac cggacctggc cgacgagcac gctgatggcg
     1681 aggtcgtgcc tctcctttag tatctccgtg gccttgcggg ctgcctcgag cgcgaacctc
     1741 cgcgtttcct ccgggccgct ggtctcctcg aggtaggtgg ccagggccct gaccgcccgt
     1801 gccaggtcct gcacggcctc gggcagcggc ggcgggatgg tgtcgccgcg cctgagcgcg
     1861 ttggccgcgc cgcgcgccag gacccgcgtg ttgatgacgg ccaggtctat cctggtgccc
     1921 gcgctggcgt agagctcgag gtgctcgagg gccctgcggc gggtcgggga gagctgggcg
     1981 gtctcgtagc cggcagcgag ggcctcgtag aagttcgtca cccggtcgtc gtcgatggtt
     2041 ctggcgcgcg cgagcgctcg ttcgacggcc tcgcggtctc cgtctcggag cgccgtggag
     2101 atctcctcca gcacagctgc cagc
//
LOCUS       DASTUV010000400         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_495492, whole genome shotgun sequence.
ACCESSION   DASTUV010000400 DASTUV010000000
VERSION     DASTUV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495492"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFT03_08095"
     CDS             complement(<1..1246)
                     /locus_tag="VFT03_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4848145.1"
                     /translation="MTHRIRRVAVLGAGTMGAAIAAHCANAGLEVDLLDIAPDEPDAN
                     KNDVVNAGFDRMKNARPAALMSGRVAERIRLGNFDDDFDRVAEADWVLEAILEKLEPK
                     RELMQRVENVAGNDALISSNTSGIPLHQIADGRSEGFRKRFLGTHFFNPPRYLKLLEI
                     IPTEDTDPEVVEFVRDFGERVLGKGGVIAKDTPNFIGNRLGSFTGMQSVTYALENGYG
                     VEEVDAITGPLIGHPRTATFRLNDQVGLDIAVGVAENLVEAAPEDESREDIRPHPKLK
                     EMLDKNLLGNKTGAGFYKRSKRDGKTVFEVLDLETFEHHPPENPEIPIAREARERGDL
                     GARLRFLMDRADEDRHARFIRDTLLPYMAYASRRVPEISDTLEDVDHAMEWGFVHQTG
                     PFRTWDLLGVRQTARRMDEMGIE"
     gene            1359..2540
                     /locus_tag="VFT03_08100"
     CDS             1359..2540
                     /locus_tag="VFT03_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4848146.1"
                     /translation="MLADVTRSLGTDYYLIDELLTEEERGIRDRVRSFADRDVIPVIN
                     DYWEKAEFPFELIPKIAELNIAGGTIEGYGCPGMSEVASGLVAVELARADGSLNTYYG
                     VHSGLAMGTIAMLGSEEQKEKWLPPMARCEKIGAFGLTEPNHGSDAVMLETRARREGD
                     EYIIDGAKRWIGNATFADVIIIWARDDEDNVGGFLVEKGTPGYSAEVIKGKTSKRAVW
                     QADVRLEGVRIPAENRLEYARTFKDTAKVLTATRYGVAWEAIGHAVASYEAALTYAKE
                     RVQFGKPIANFQLIQSKLADMLAEITNMQLMCLRLSQLLQEGKMTGGMASLAKMNNAR
                     KARKVCSDARDILGGNGILLEYHVARHTADIEAVYTYEGTDTVQALIVGREITGLNAF
                     A"
     gene            2645..>3437
                     /locus_tag="VFT03_08105"
     CDS             2645..>3437
                     /locus_tag="VFT03_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU4848147.1"
                     /translation="MKAMVLHEIGGPEKLSYEDAPDPEPGPGESVVRLHAAALNRRDV
                     FVTKGQYPGAKPEALPVILGSDGSGEVVARGDGAGGPDEGTEVVINPALYWGEDPQKP
                     GKEYRILGLPDDGTFAQLVKVPSENVFPKPSHLSHEEAAAIPLGALTAYRALVTRGRV
                     QEGETVVVPGIGSGVATFVVQMAAALGARVFVTSGDDEKIEKAKELGAEGGVNYNSED
                     WSRELKSMSGGVDLAVDHVGGEAFDAMVSLARPGSRIVTFGATAGP"
BASE COUNT          633 a         1085 c         1132 g          587 t
ORIGIN      
        1 cttcgatgcc catttcgtcc atcctccggg cggtctgcct gacgccgagc agatcccagg
       61 tgcggaaggg gccggtctgg tggacgaagc cccactccat cgcgtggtcc acgtcctcaa
      121 gggtgtctga gatctccggc accctgcgtg aggcgtacgc catgtacggc agcagcgtgt
      181 cccggatgaa gcgggcgtgg cgatcctcgt cggccctatc catcaggaag cgcaggcgcg
      241 cgccgagatc acccctttct cgagcttccc tggctatagg gatctccggg ttctccggcg
      301 ggtggtgctc gaaggtctcg aggtccagca cttcgaagac ggtcttgccg tcgcgcttgc
      361 tccgtttgta gaagccagcg ccggtcttgt tgccgaggag gttcttatcg agcatctcct
      421 tcagcttcgg gtggggtctt atgtcttcgc gcgactcgtc ttcgggagcg gcctcgacca
      481 ggttctcggc gacaccgacg gcgatgtcga ggccgacctg gtcgttcagg cggaaggtgg
      541 cggtcctggg gtggccgatc aggggccctg ttatggcgtc aacctcctcc accccgtagc
      601 cgttctccag ggcgtaagtc acagattgca tcccggtgaa gctcccgagc ctgttcccga
      661 tgaagttcgg cgtgtccttg gcgatgacgc cgcccttgcc gagcacccgc tccccgaaat
      721 cgcggacgaa ctcgacgacc tcggggtctg tgtcctccgt ggggatgatc tcgagcagct
      781 tcaggtagcg tggcgggttg aaaaagtgcg tgccgaggaa acgcttcctg aacccctcgc
      841 tacggccatc cgctatctgg tgcagcggta tgccggaggt gttggatgag atcaaggcgt
      901 cattaccggc gacgttctcc acccgctgca tgagctcccg cttgggttcg agcttctcca
      961 ggattgcttc gaggacccag tccgcctcgg ctacccggtc gaagtcgtcg tcgaagttgc
     1021 cgaggcgaat gcgctctgcg acccgcccgc tcatcagcgc ggccggcctg gcgttcttca
     1081 tccggtcgaa gcccgcgttg acgacgtcgt tcttgttcgc gtcgggttcg tccggcgcga
     1141 tgtctagcag gtccacctcg aggcccgcgt tggcgcagtg ggcggcgatg gcggctccca
     1201 tcgttcccgc gccgagcacc gccacccggc gtatccggtg tgtcatcttc tctccccgta
     1261 gtcgcagcgt ctaccagtcc gtagaagata gcatctctct acacgggtat cgtagagaga
     1321 tgctagagtt tgcggggcgt agagaaggag gagatcactt gcttgccgac gttacccggt
     1381 cccttggcac cgattattac ctcatcgacg agttgttgac ggaggaggag aggggaatcc
     1441 gggacagggt ccgttccttc gccgaccggg acgttatccc ggtgatcaac gactactggg
     1501 agaaggccga gtttccgttc gagctgatcc ccaagatagc cgagctgaac atcgcgggcg
     1561 gtaccataga aggctacggc tgcccgggga tgagcgaggt cgcctccggg ctcgtggccg
     1621 tcgagctcgc ccgcgccgac ggtagcctga acacttacta cggagtccac tcggggcttg
     1681 cgatgggcac catcgccatg ctcggctcag aggaacagaa agagaagtgg ctcccgccga
     1741 tggcgcggtg cgagaagatc ggggccttcg ggctcaccga gccaaaccac ggctcggacg
     1801 ccgtgatgct cgagaccagg gccaggcgcg agggcgacga gtacataata gacggcgcga
     1861 aacgctggat cggcaacgcc accttcgccg acgtcataat tatctgggcg cgcgacgacg
     1921 aagacaacgt cggcggcttc ctcgtcgaga agggcacgcc cggctactcg gccgaggtaa
     1981 taaagggcaa gacctccaag cgcgccgtgt ggcaggccga cgtgagactg gaaggcgtaa
     2041 gaattccagc cgaaaaccgg ctcgagtacg cccgcacttt caaggacacg gcgaaggtac
     2101 tcaccgccac ccgctacggg gtggcgtggg aggccatcgg gcacgccgtc gcctcatacg
     2161 aggcggcgct gacctacgcc aaagagcggg tccagttcgg caagccgata gccaacttcc
     2221 agctcatcca gagcaagctc gccgacatgc tcgccgagat caccaacatg cagctcatgt
     2281 gcctgaggct cagccagcta cttcaggaag gcaagatgac aggcggcatg gcctctctgg
     2341 ccaagatgaa caacgccagg aaagcgcgca aggtttgctc cgacgcgcgc gacatcctcg
     2401 gagggaacgg catcctgctc gagtaccacg tcgccaggca caccgccgac atcgaggccg
     2461 tctacaccta cgagggcacg gacaccgtcc aggcgctaat agtggggcgc gagatcacag
     2521 gcctcaacgc ttttgcgtag gcttcaggaa ggcttatggc acgaccagcg agcgcggcgt
     2581 cggcccctat taacctgaag cctgaaacca gacacctgat agctgagcga ccgaagggag
     2641 caacatgaaa gccatggtac tgcacgagat cggcggcccc gagaagctaa gctacgagga
     2701 cgcccctgac ccggaacccg gcccgggtga gtcggttgtc aggctgcacg ccgcggccct
     2761 aaaccgccgc gacgtgttcg tgacgaaggg ccagtacccc ggcgcgaagc ccgaggcgct
     2821 tcccgtcatc ctaggctcgg acggctcggg cgaggtagtc gcccgcggcg atggcgcagg
     2881 cggaccagac gaggggacgg aggtggtcat caaccccgcg ctctactggg gcgaggaccc
     2941 gcagaagccc ggcaaagagt accgtatcct cgggctcccc gacgacggca cgttcgccca
     3001 gctcgtcaag gtgccctcgg agaacgtctt cccgaagccc tcgcacctct cgcacgagga
     3061 ggccgccgcg atacccctgg gcgccctcac cgcttacaga gcgctagtca ctcgcggcag
     3121 ggttcaggaa ggcgagaccg tggtcgtgcc cggcatcggg agcggggtgg ctacgttcgt
     3181 cgtgcagatg gccgccgctc tgggagcgcg ggtcttcgtc acctcgggcg acgacgagaa
     3241 gatcgagaag gcgaaggagc tcggcgcaga gggcggcgtc aactacaact cggaggactg
     3301 gtcaagggag ctgaagagca tgagcggagg cgtggacctg gcggtggatc acgtcggcgg
     3361 tgaagccttc gacgccatgg tctcgctcgc caggccaggg agccgcatcg tcactttcgg
     3421 ggcgacggcg ggtccga
//
LOCUS       DASTUV010000401         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_552867, whole genome shotgun sequence.
ACCESSION   DASTUV010000401 DASTUV010000000
VERSION     DASTUV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552867"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFT03_08110"
     CDS             <1..730
                     /locus_tag="VFT03_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU4848148.1"
                     /translation="FERGMERLHMKVPYGRAEIQEILTECVRLSGLREAYVEVVCTRG
                     VPPPGSRDPRECENRLYAFAVPFVWIADSEKQERGLDAVIGRVQRIQPEAVDPTVKNY
                     HWLDLVAGLYEAYDRGGETAILTDSEDNVVEGPGFNVFAVNDGNISTPSRGVLEGITR
                     RTVIELASERGMPLGARAVPAAEVRAADEVFISSTAGGIMPVTKVDGAPVGDGTPGPV
                     TLRLRDAYWELHRDPRYVTPVRYD"
     gene            768..1220
                     /locus_tag="VFT03_08115"
     CDS             768..1220
                     /locus_tag="VFT03_08115"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU4848149.1"
                     /translation="MATMPQQGQQLPDVEFVTEGGEKLSAGDLEGEKTVLYFYPKDDT
                     PGCTKEACAFRDRMSDYERAAIRVYGVSLDSPESHRRFREKYNLNFPLLTDEGGRAAD
                     ALGVLRESGKSANRVTFLLDSNGRIARVYPEVSPETHADEILEDAASL"
     gene            1275..1877
                     /locus_tag="VFT03_08120"
     CDS             1275..1877
                     /locus_tag="VFT03_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU4848150.1"
                     /translation="MEQEAEMSVEERGDRFRELEEAYRDYTVYDQHYERVGRVDDLFV
                     DENDQPEYIGVKTGLLGMKSTLIPMEIARVNDRRKLIEVATDRHTITEGPAFDDNEEI
                     TRDHEARIYGYYGLHHPGFQEGTGGYGDYYASETEGRTGDDLAGAVDTEYGQRREESL
                     EDSRQGTSTGEPAPDHPDTSGASAGEQGGVRVYKRVRTGR"
     gene            complement(2101..>2281)
                     /locus_tag="VFT03_08125"
     CDS             complement(2101..>2281)
                     /locus_tag="VFT03_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848151.1"
                     /translation="LHLAANGSVELGQEVTVVLAGDATELVIDDNAQRVEGLGVPPVR
                     ELLAKLRDHQVPVYV"
BASE COUNT          439 a          731 c          769 g          342 t
ORIGIN      
        1 cttcgagcgc gggatggaga ggctgcatat gaaggtgccg tacgggcgcg cggagataca
       61 agagatcctt acggaatgcg tccgcctgag cggcctgcgc gaggcctacg tcgaggtagt
      121 gtgcacccgc ggagtcccgc cgcccggctc gcgcgacccg cgcgagtgcg agaacaggct
      181 ctacgcgttc gccgtgccgt tcgtctggat cgccgactcc gagaagcaag agcgggggct
      241 cgacgccgtg atcgggcgcg tgcaacgcat ccagcccgag gccgtcgacc ccacggtcaa
      301 gaactaccac tggcttgacc tggtggccgg gctctacgaa gcctacgacc gcggcgggga
      361 gacggcgatc ctcaccgaca gcgaggacaa cgtcgtcgaa ggcccgggct tcaacgtctt
      421 cgctgtgaat gacggcaata tctccacgcc gtcgcggggt gtcctcgagg gtataacccg
      481 cagaaccgtc atcgagctgg cgtccgagcg cggcatgccg ctcggagcca gggcggtccc
      541 cgcggccgaa gtgagggcgg ccgacgaggt cttcatctcc agcacggcgg gtgggatcat
      601 gccggtaacg aaggtggacg gcgcacccgt aggcgacggc acgccaggcc ccgttaccct
      661 gcgcctgcgc gacgcctact gggagctgca cagggatccc cgctacgtca cgcccgtccg
      721 atatgattaa actcacgctc gagaatgtac gctacggaag gagtcacatg gcgacgatgc
      781 cgcagcaggg ccagcagcta cccgacgtcg agttcgtcac cgagggcgga gagaagctct
      841 cggccgggga cctcgagggt gagaagaccg ttctgtactt ctatccgaaa gatgacaccc
      901 cgggctgcac caaagaggcg tgcgcgttca gggaccgcat gtccgactac gagcgggcgg
      961 ccatccgggt ctacggcgtc tcgctcgact cacccgagtc gcaccggcgc ttccgcgaga
     1021 agtacaacct gaacttcccg ctcctcaccg acgagggcgg ccgcgcggcg gatgctctcg
     1081 gggtgttgcg cgagagcggg aagtcggcga accgcgtcac ctttctgcta gattccaatg
     1141 gaaggatcgc cagggtctac cccgaggtct ctcccgagac gcacgcggac gagatcctcg
     1201 aggatgccgc ctcgctatga gccccgcgtt tcggggtttg ggggaggggg taagcaccac
     1261 tccgagatgt gggcgtggag caggaggctg agatgagcgt cgaggagcgc ggggatcgat
     1321 tccgggagtt ggaagaagcg tacagggatt acacggtcta tgaccagcac tacgagcggg
     1381 taggtagggt ggacgacctg ttcgtagacg agaacgacca gcccgagtac atcggcgtga
     1441 agacaggctt gcttgggatg aagtccaccc tcatcccgat ggagatagcc agggtaaacg
     1501 acaggcgaaa gctcatagag gtcgccacgg acaggcacac gatcacggaa ggcccggcct
     1561 tcgacgacaa cgaggagatc acgcgcgacc acgaggccag gatctacggt tactacggcc
     1621 tgcatcatcc cggcttccag gagggaaccg gcggctacgg cgactactac gcctccgaga
     1681 ccgaaggacg gaccggcgat gacctggctg gagcggtcga caccgagtac gggcagcgca
     1741 gggaagagtc gctcgaggac tccaggcagg gaacgtccac aggcgagccg gctccggatc
     1801 acccggacac atcaggtgcc agcgccggcg agcaaggtgg agtgagggtg tacaagcggg
     1861 tcaggaccgg ccgctagcaa aatcccgcgg tcgccaccga ggcacagcct tcctcgaaac
     1921 gatcgatctc cgcctcgaga atcccccgtt gggcctccgc acaccgctct agaacgtgag
     1981 cgccctgtct gcctcggcag cgagcctcgc cacgtcagtc ggggctgccc acctgccgtt
     2041 gatcgtgtcc cgcaggctct cctcgtcttc ggcggcccca cgggccaccg cgcacccctt
     2101 tcagacgtag acggggacct ggtgatctct cagcttggcg agcagctctc ggacgggggg
     2161 tacgcccaga ccctccacgc gctgcgcgtt gtcgtcgatc accagttcgg tagcgtctcc
     2221 ggcgagcacc acggtgacct cctgcccgag ctcgactgat ccgttggcag ccaggtgcag
     2281 t
//
LOCUS       DASTUV010000402         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1493248, whole genome shotgun sequence.
ACCESSION   DASTUV010000402 DASTUV010000000
VERSION     DASTUV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493248"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFT03_08130"
     CDS             <1..304
                     /locus_tag="VFT03_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4848152.1"
                     /translation="GTGLFRVLFEPGARTNWHTHPEGQFLYVVTGAGRAGTEGGPVME
                     IQAGDVVFFAPDEKHWHGAGPDTFMVHIAITPAISTEGGTDWLEPVADEDYSGPPL"
     gene            complement(301..1338)
                     /gene="nrfD"
                     /locus_tag="VFT03_08135"
     CDS             complement(301..1338)
                     /gene="nrfD"
                     /locus_tag="VFT03_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU4848153.1"
                     /translation="MTDVGQETSRQQNGKVETSHHPEADSRRGDKNYYNIPPIKRAHW
                     TWQIPVYFWIGGIGAGVHLFSTIAQLLGHEDRALVRTSRYTVLLTMILSPVLLIWDLG
                     RPERFYNMLRILKLRSPMSTQSWSLFIFGNLAGLIAARQAAEDGLLGRSALSKLVIRL
                     VPTRLLSVLALPFWLFVGFNTGNLISATSVPRWARNWLFMAPTFLTSGLSTALSLLSL
                     VLHLGHRGEKKTLRALRRAEKATLVLEAVLFAASLARMSRWSKPLFSKKVAPLFVGGG
                     IIAGIAAPMALLFGREARGKSILASVLVLLGGLAFRFAMIMGGRISADDPEAYFTFAS
                     GENAPQPEDKI"
     gene            complement(1349..2185)
                     /locus_tag="VFT03_08140"
     CDS             complement(1349..2185)
                     /locus_tag="VFT03_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU4848154.1"
                     /translation="MATGFLTDTTVCIGCKACEVACKQWNQLPADGYELSGDSYDNTR
                     QLSGTTWRHVAFIENPKKKRLTGDVDLVALAEDVREELPGGAELDALGRWTFMSDVCK
                     HCVEAPCQQACPTGAIIRNEFDNVYIQPDVCNGCGYCVPACPYGVITQVPHDGRAFKC
                     TLCYDRQKDGLEPACAKACPTNSIMFGEVEELEQMAEERVATLHARGYTNAFVYGTPE
                     VGGTGGIGGNHSKFILMDEPETYNLPKHPYLPQEKVKGGVIAGAVAAGVLGAVVALAF
                     RD"
     gene            complement(2185..2520)
                     /locus_tag="VFT03_08145"
     CDS             complement(2185..2520)
                     /locus_tag="VFT03_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848155.1"
                     /translation="MRDSRGTRTTPEDTGSDTYRVETSHREMMQQELMDLGVWLIEHP
                     EAAVRNLEPIRSARVLEPGVYYNVGTGMVDRIFAPQHVPLGHRLFRVSSDPAAPVEEI
                     RRKVMEGKG"
     gene            complement(2517..2984)
                     /locus_tag="VFT03_08150"
     CDS             complement(2517..2984)
                     /locus_tag="VFT03_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848156.1"
                     /translation="MDARERILAGTLGGAVGTLALSGLREAFSRMGLVFETAPMQVVD
                     RAQEVGLVDGWSPRALRLLTVAAHIAYGVGAGMALGLLRRERAGAGQEAAVGSAMGVL
                     VWGAGWSTWLPLTGVHSPPWEQRSARVLLPVIDHAVYGAIWGISFWILTRRRA"
     gene            complement(3005..>3822)
                     /locus_tag="VFT03_08155"
     CDS             complement(3005..>3822)
                     /locus_tag="VFT03_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU4848157.1"
                     /translation="APAWGWAWPMNRRILYNRASADPEGRPWSERKKYVWWDEDQEKW
                     VGEDVPDFQVDKPPTYRPDTDAKGVDAIPGDGPFIMQGDGLGWLFAPVGLKDGPLPVH
                     YEPVESPVPNLLYERRRDPAAELFDRPDNPYNPPEDPRYPYVVTTYRLTEHHTSGAMS
                     RWIPWLSELQPELFAEISPELAAEKGVDNTDWVTIETSRGKIHCKALVSERIPVFDVD
                     GKKIHTIGLPYHWGSNGVVKGDVVNELVPVALEPNVSIHEAKAFTANLHKGRA"
BASE COUNT          624 a         1247 c         1276 g          675 t
ORIGIN      
        1 aggcacgggg ctgttccgcg tgctcttcga gccgggggcg cgcacgaact ggcacaccca
       61 cccggagggg cagttcctct acgtcgtcac aggcgccggt cgcgcgggga ccgagggcgg
      121 gcccgtgatg gagatccaag ccggagacgt cgtgttcttc gctccggacg agaagcactg
      181 gcacggcgcg gggccggaca ccttcatggt ccacattgcc ataacgccgg ctataagcac
      241 cgaaggcggc accgactggc tcgagccggt cgcggacgag gattactcgg gaccgccgct
      301 ctagatcttg tcctccggtt gcggggcgtt ttcgccgctg gcgaaggtga agtaggcttc
      361 ggggtcgtcg gcgctgatgc gcccgcccat gatcatagcg aagcggaagg cgagcccgcc
      421 gagcaggacg aggacggagg cgaggatgct cttgccacgc gcctcgcgcc cgaagaggag
      481 cgccatcggc gccgctatac ccgctattat gcccccgccg acgaagagcg gggcgacctt
      541 cttcgagaac agcggcttgc tccaccggct catgcgcgcc agcgaggccg cgaagagcac
      601 cgcttcgaga accagggtgg ccttctcggc gcggcggagg gcgcgcaggg ttttcttctc
      661 gccccggtgc ccgaggtgga gcacgagcga gaggagcgag agggccgtgg agaggcctga
      721 ggtcaggaag gtcggggcca taaacagcca gttgcgcgcc cacctcggga cggaggtggc
      781 gctgatcagg ttgcccgtat tgaacccgac gaagagccag aacggcagtg cgaggaccga
      841 gagtagacgg gtcgggacga gccggatcac gagcttcgag agggcgctcc tccctaacaa
      901 tccgtcttca gcggcctggc gggctgcgat caagcccgcg aggttgccga agatgaagag
      961 cgaccagctc tgggtggaca tcggcgagcg cagcttgagg attcggagca tgttgtagaa
     1021 gcgctcaggg cgtcccaagt cccagatcag gagcacaggg ctcaggatca tcgtgaggag
     1081 cacggtgtac cggctcgtac gtacgagcgc cctgtcttcg tgaccgagga gctgggcgat
     1141 ggtcgagaac aggtggaccc ctgcccctat acccccgatc cagaagtaca cggggatctg
     1201 ccacgtccag tgggcgcgct tgatcggggg aatgttgtag tagtttttat cccccctgcg
     1261 cgagtcggcc tcggggtggt gggaagtctc gaccttcccg ttctgctgcc gagatgtctc
     1321 ctggcccacg tctgtcatcc gtcccctcct aatcccggaa agccagcgcc acgacggccc
     1381 cgaggacgcc ggccgcgacc gcaccggcta tgacgcctcc cttgaccttc tcctgcggca
     1441 gataagggtg ctttggaagg ttgtaggtct cgggctcgtc catgaggatg aacttggagt
     1501 ggttgccccc tatgccgccc gtaccgccga cctcgggcgt gccgtagacg aatgcgttgg
     1561 tgtagcctct ggcgtgcagg gtggcgacgc gctcttcggc catctgctcc agctcttcga
     1621 cctcgccgaa catgatggag ttggtagggc acgccttggc gcaggccggc tcgaggccat
     1681 ccttctgccg gtcgtagcat aaagtgcact tgaaggcccg gccgtcgtgg gggacctggg
     1741 ttatgacgcc gtaggggcag gcggggacgc agtagccgca gccgttgcag acgtcgggct
     1801 ggatgtagac gttgtcgaac tcattgcgga tgatcgcgcc ggtggggcac gcctgctggc
     1861 aaggcgcctc gacgcagtgc ttgcagacat cgctcatgaa cgtccagcgc cctaaagcgt
     1921 cgagctcggc cccgcccgga agttcctcgc gcacgtcctc ggctaaggcg acgaggtcca
     1981 cgtcgcctgt gaggcgcttc ttcttcgggt tctcgataaa ggcgacgtgg cgccaggtgg
     2041 tgccggagag ctgcctggta ttgtcgtagg agtcgcccga gagctcgtag ccgtctgccg
     2101 ggagctggtt ccactgcttg caggccacct cgcaggcctt gcacccgatg cagaccgtgg
     2161 tgtccgtcaa gaatcctgtc gccactagcc cttcccttcc atgactttac gccgtatctc
     2221 ctccaccggc gccgccgggt cgctgcttac gcggaacaaa cggtgcccga ggggcacatg
     2281 ttggggggcg aagatcctgt cgaccatccc cgtgcccacg ttgtagtaga cccctggctc
     2341 gagcaccctg gcgcttctta tgggctcgag gtttctgacg gcggcctcgg ggtgctcgat
     2401 gagccatacc ccgaggtcca tgagctcctg ctgcatcatc tccctgtggg aggtctccac
     2461 ccgataggtg tcgctccccg tgtcctcggg agtggtgcgc gtccccctgg aatctctcat
     2521 gcgcgcctcc gggtgagtat ccagaagctg atgccccaga tggccccgta caccgcgtgg
     2581 tcgatcactg gcagcaagac cctcgcgctc cgctgctccc acggcggaga atggacgccc
     2641 gtcagcggca gccaggtaga ccaccccgcg ccccacacca ggacccccat agcggagccc
     2701 acggcggcct cctggccggc cccggccctc tcacggcgca gcagacccag agccatcccc
     2761 gcgccgacgc cgtaggcaat gtgcgcggcc acggtaagga gacgcagggc tctcggcgac
     2821 cagccgtcca cgaggccgac ctcctgcgcg cgatccacga cctgcatcgg ggcggtctcg
     2881 aagaccagtc ccatcctgct aaatgcctcg cgtagccccg acaacgcgag cgtccccacg
     2941 gctcctccga gggtccccgc caggatcctc tctcttgcgt ccatgctctc ctatccggcc
     3001 ccactcatgc gcggcctttg tgtaggttgg cggtgaaggc tttagcctcg tggatggaga
     3061 cgttcggctc caacgcaacc ggaacaagct cattgaccac gtcgcccttg accaccccgt
     3121 tcgatcccca gtggtaggga agcccgatgg tgtggatctt cttcccatcg acgtcgaaga
     3181 cggggatccg ctcggagact agcgccttgc agtggatctt gccgcgcgag gtctcgatgg
     3241 tcacccagtc ggtgttgtcg acacctttct cggcagccaa ctcgggactt atctcggcga
     3301 acagctcggg ctgcagctcg gagagccagg ggatccagcg gctcatcgcc ccgcttgtgt
     3361 ggtgctcggt gaggcggtag gtcgttacca cgtagggata ccgcgggtcc tccggagggt
     3421 tgtaggggtt gtcgggacgg tcgaagagct ctgccgccgg gtctcgccgc cgctcgtaga
     3481 gcaggttcgg gaccggcgac tccaccggct cgtagtggac cgggagcggt ccatccttga
     3541 gccccacggg ggcgaagagc cagccgagtc cgtcgccctg catgatgaac gggccgtcgc
     3601 cgggaatcgc gtcaacaccc ttggcgtcgg tgtcggggcg gtaagtcggt ggcttgtcca
     3661 cctggaagtc ggggacgtcc tcgccaaccc acttttcctg gtcctcgtcc caccagacgt
     3721 atttcttgcg ctccgaccag ggtctaccct cggggtcggc agaggcgcgg ttgtagagga
     3781 tgcggcggtt catcggccac gcccagcccc acgcgggagc ga
//
LOCUS       DASTUV010000403         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1404292, whole genome shotgun sequence.
ACCESSION   DASTUV010000403 DASTUV010000000
VERSION     DASTUV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404292"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFT03_08160"
     CDS             <1..417
                     /locus_tag="VFT03_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848158.1"
                     /translation="NALGIGLADFTTQRLFEKIDYEAMNQNVLTSTFVERAKIPMIME
                     NEREALQAAVRCNWGVEPEDTRFVRIPNTLHLRYAYLSENLLDEALANGNVEVVEGAA
                     DLAFDESDDLVHFDAEAEEQTVAVSSNRDDGYYGGD"
     gene            459..1688
                     /gene="glp"
                     /locus_tag="VFT03_08165"
     CDS             459..1688
                     /gene="glp"
                     /locus_tag="VFT03_08165"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU4848159.1"
                     /translation="MQLFEKLIEHPEAVRLVLEHTHRLPADDVPLVEARGLALAEDLE
                     ARFDSPPFDNSAVDGYAVRSADAEVGRTLRVVDEAPAGRPAAKSVGEGEAIKIFTGGV
                     IPEGADAVVMVENTSGWGEEFELKKAASSGQNVRGSGEDVREGNVILSAGTEVGPPEI
                     ALAATQGYGRLPVYRRPEVVVLSTGTELVEPGERGLSPGEIYDSNSFALIAQAEEVGA
                     RARRITAASDDATVLRNAVREALEIADVVVTSGGVSVGEKDLVKATMLELGVEQVFWG
                     VKFKPGKPLFFGVREGASVFGLPGNPVSAMVCFELFVRPALVRMMGREDRGRPRIQVY
                     FEEDVKNNFGRMHAMRVSLERTEKGWLARSVGAQGSGLVSSLTKADALALIGPESEGV
                     AAGEPVEAIVLREGVIL"
     gene            1746..>2182
                     /locus_tag="VFT03_08170"
     CDS             1746..>2182
                     /locus_tag="VFT03_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848160.1"
                     /translation="MFESEFEDNTFRRRLMKEVAPGVPVHLSFYSPSVKVQVQPFAEH
                     LPGANGRYVGDAVTIEFVPDNAAAVERVREFVWYWEEYNECTGVRKINHLRRSVSPES
                     VQGILERVRGVDKRAAAKAAEAVELEELLDLIAEKDAKTLARLP"
BASE COUNT          414 a          631 c          791 g          346 t
ORIGIN      
        1 aacgctctgg gtatcggtct tgccgatttc acgacacaga ggctcttcga gaagatagat
       61 tacgaggcca tgaaccagaa cgtcctcacg agcactttcg tcgagcgggc caagatcccg
      121 atgatcatgg agaacgagcg cgaggcgctt caggccgccg tccgctgcaa ctggggggtc
      181 gagccggagg acacccgctt cgtccgtatc cccaacacgc tccacctccg ctacgcctac
      241 ctctccgaga acctcctcga cgaggccctc gcgaacggca acgtcgaggt cgtcgaaggc
      301 gcagccgatc tggctttcga cgagagcgat gatcttgtgc acttcgacgc cgaagccgaa
      361 gaacagacgg tggccgtctc ctccaaccgg gacgacgggt actacggggg cgactagcgt
      421 ccggaagacc atagaccgct aaatagtcag gaacgagcat gcaacttttc gagaagctca
      481 tagagcaccc cgaagccgtg aggctggtgc tcgagcatac gcaccgtctg cccgcagatg
      541 acgtgcccct ggtggaagcg cgagggctcg cgctggcgga ggacctcgag gccaggttcg
      601 actcgccgcc gttcgacaac tcggctgtgg acggttacgc ggttaggagc gcggacgccg
      661 aggtgggtcg caccctcagg gtggtggacg aggcgcccgc cggacgtccg gccgccaaga
      721 gcgtcgggga gggcgaggcc atcaagatct tcaccggcgg ggttatccct gaaggcgcgg
      781 acgcggtcgt gatggtcgag aacacctccg gttggggcga ggagttcgag ctgaagaagg
      841 cagcctcttc ggggcagaac gtgcgcggaa gcggcgagga tgtgcgcgag ggtaacgtta
      901 tcctgagtgc ggggacggag gtgggtccgc ccgagatagc cctggctgcg acgcagggct
      961 acgggaggct gcctgtctac aggcgtcccg aagtggtcgt cctctcgacg ggcaccgagc
     1021 tggtcgagcc aggggagcgg ggcctctcgc caggcgagat ctacgactcc aactccttcg
     1081 ccctgatcgc ccaggccgag gaggtcggag ccagggctcg ccgcataacc gccgcctcgg
     1141 acgacgccac agtgttgcgg aacgccgtca gagaagccct cgagattgcg gacgtggtgg
     1201 tgacgagcgg cggggtctcg gtgggggaga aggacctggt caaggccacc atgctcgagc
     1261 tcggcgtgga gcaggtcttc tggggcgtca agttcaaacc cggcaagccg ctgttcttcg
     1321 gggtgcgcga aggcgcgagc gtcttcggtc tgcctgggaa cccggtctcc gcgatggtct
     1381 gcttcgagct cttcgtcagg cctgcgctgg tgaggatgat ggggcgcgag gacaggggaa
     1441 gaccccggat acaggtgtac ttcgaggagg acgtcaaaaa caacttcggg cggatgcacg
     1501 cgatgcgtgt gagcctcgag cgaacggaga aaggctggct cgcccggtcc gtcggggcgc
     1561 agggatcggg gctcgtgtcc tctctcacca aggccgacgc gctggctctc atagggccgg
     1621 agtccgaggg ggtggcggcg ggggagcccg tggaggccat cgtgctgcgc gagggagtaa
     1681 tcctgtgata gaaggtgaca cagaggggat ggttcgggga ccggtgatgg tagaatacgg
     1741 tcccgatgtt tgaatcagaa ttcgaagaca atacgttccg caggcgcctg atgaaggagg
     1801 tcgcgccggg cgtaccggtg cacctctcct tctactcgcc ttctgtcaaa gtgcaggtcc
     1861 aacccttcgc cgagcacctg cccggcgcga acggccgcta cgtgggcgac gcggtgacca
     1921 tcgagttcgt acccgataac gccgcggcgg tggagcgggt gcgggagttc gtctggtact
     1981 gggaggagta caacgagtgt acgggggtgc gcaagatcaa ccacctcagg cgcagcgtca
     2041 gccccgagag cgtgcagggc atcctggaaa gggtgcgcgg cgtggacaag agggcagcgg
     2101 cgaaggccgc agaggcggtg gagctggagg aactgctcga cctcatagcg gagaaggacg
     2161 ccaagacgct ggcgcgtttg cc
//
LOCUS       DASTUV010000404         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1055549, whole genome shotgun sequence.
ACCESSION   DASTUV010000404 DASTUV010000000
VERSION     DASTUV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055549"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFT03_08175"
     CDS             complement(<1..854)
                     /locus_tag="VFT03_08175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848161.1"
                     /translation="MPRRVGKDQGAEAALARGARLGAMGLLLSGNLGISTAFGMLLLL
                     PLYVQKLGGDEASFGIVMAAATIPAGLSIGLLIRYPEAWRPNVVLTIAVLAYGLGAAG
                     AALVTVTWVPLVGIGVLLGTAWAVVYAASPMVMSEMVSDRERATYFGYLTGTQQLGIG
                     LGPVIGGLLVGGGLGFRGAFLVAGALCAVAAALVFVVGWIVPDARMTAARTGAGTGDE
                     PGTIAPFGSALGRILRSESAFWLVMIALFACLFTTMTSFQTTFAGARGLDFSVYYLSY
                     TAAVIISR"
     gene            982..1905
                     /locus_tag="VFT03_08180"
     CDS             982..1905
                     /locus_tag="VFT03_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU4848162.1"
                     /translation="MELRQVKTFRTVARELSFSRAAASLGYVQSSVSSQVASLERELG
                     VPLFDRLGRSITLTEAGRVMLVYSERLLGLAEEAEEAVADAGTGSGEIAGSLSLSAPE
                     TLLTYRLPALLALFHERYPKVRLSLKPTAVGKLLGSTRKALERGQVDVALVLDVPPVS
                     IDGLSVEVLAEEAVSVVAPSAHTLASSTVVIPSDLVGETVLLPEAPESGCAYRGQFER
                     QLSEGGVVPGETLEFQSIEAIKRCVSAGMGVSVLPTVAVQTELVAGSLSELRWREPIR
                     VGTLMAWNSERWASPALRAFLQTAREVLSAT"
     gene            2202..>2598
                     /locus_tag="VFT03_08185"
     CDS             2202..>2598
                     /locus_tag="VFT03_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4848163.1"
                     /translation="MSANPQDGSRPTVVLVHGAFADGSSWNEVIERLQAEGVHVTAPA
                     NPLRGIAIDSAYINSFFEQIPGPVLAVAHSYGGAVITNAATNANNVLGLVYVAAFAPD
                     EGETLGAIASNSTDSVVLPALVELQYPTGE"
BASE COUNT          501 a          831 c          816 g          450 t
ORIGIN      
        1 aaacgggaga tgatcacggc cgccgtatag gagagatagt atacggagaa gtccagtccc
       61 cgcgccccgg cgaaggtcgt ctggaagctc gtcatggtgg tgaacaggca ggcgaagagg
      121 gcgatcataa ccagccagaa tgcactctcc gagcggagta tacgccctag cgcggacccg
      181 aaaggagcta tagttccagg ctcgtcaccg gtgccagcac cggtcctggc agccgtcatc
      241 ctcgcgtcgg ggacgatcca gcccaccacg aacacgaggg ccgccgcgac agcgcagagc
      301 gcgcctgcta ccaggaaggc gccccgaaag ccgagcccac ctcccactag caagccgcca
      361 ataaccggcc cgaggccgat cccgagctgc tgggtgccgg tcaggtagcc gaagtaggta
      421 gctctttccc ggtcggacac catctcgctc atcaccatcg gagaggccgc gtagactacg
      481 gcccaggccg tgccgagcaa cacccctatc ccgaccaggg gcacccacgt gaccgtcacc
      541 agcgccgccc ccgccgcccc gagcccgtag gcgaggacgg cgatcgtcag caccacgttc
      601 ggtctccagg cttccgggta gcggatcaag agcccgatcg acagtcccgc cggtatcgtg
      661 gcggccgcca tgacgatccc gaagctcgcc tcgtcacccc cgagcttctg gacgtacagg
      721 ggcagcaaga ggagcatccc gaaggccgtc gagataccca gattgcccga gagtagaagt
      781 cccatcgcgc ccaacctcgc cccacgcgct agagcagctt cggcgccctg atccttgcca
      841 acccttctgg gcatcctgcc ttctccttgt gctcgttgcc cctgttgact cgatggtact
      901 ggcgcagtac catcgaatcc aacgaaagtt ttagatcgta tctatctgcg aaaccgataa
      961 cataaccaag gagctgtgac gatggagctc aggcaggtaa agaccttcag gactgtggcg
     1021 cgggagctct ccttttcccg ggccgccgcc agtctcggct acgtgcagtc gagcgtaagt
     1081 tcgcaggtgg cttctctgga acgggagctc ggggtacccc tctttgacag gttgggaagg
     1141 agtatcacgc tcaccgaggc cgggagggtg atgctagttt attccgagag gctgctcggt
     1201 ttggccgagg aggccgagga ggcggtcgcg gacgcgggga ctgggtcggg cgagatcgca
     1261 ggttccctga gtctcagcgc gccggagacc cttctgacct accggcttcc cgcgctcctg
     1321 gccctcttcc acgagcgcta tcccaaggtg aggctctctc tgaagccgac cgccgtcggc
     1381 aagctcctgg gctcgacgcg gaaggccctc gaaaggggcc aggtggacgt cgccctcgtc
     1441 ctggacgtac caccggtgag catcgacggt ctctcggtag aagtgttggc ggaggaggcc
     1501 gtgagcgtcg tggcgccgtc cgcgcacacg ctcgcgagct ccaccgtggt aatcccctcg
     1561 gatctggtcg gggagaccgt gctcttgccg gaggcgccgg agtccgggtg cgcctaccgc
     1621 ggccagttcg agcggcagct ctcggaggga ggcgtggtgc caggggaaac gctggagttc
     1681 cagagcatag aggccataaa gcggtgcgtg agcgcaggca tgggcgtctc cgtcttgcct
     1741 accgtggccg tgcagaccga actcgtggcc ggcagccttt cggagctgag gtggcgggag
     1801 cccatccgcg tcggcactct gatggcgtgg aactcggagc ggtgggcgtc gccagccctg
     1861 cgggccttcc tccaaacggc gcgggaggtc ttatccgcta cttgagctcg ctgtggtcgg
     1921 gagtggcttt ccaagcggtt tcagaaacag aatatctgga gaatcctcag tcatgctact
     1981 aatttggtga gctagagcac tttgtgctgt gctgaacaca ctttctcttt tcacaggagt
     2041 atcgtgcgat cgtttcgcaa aagaagcgcg atccgcgtaa aggagaccac catggccacg
     2101 gacaccataa tcgctttcga ggtgcaaccc tttcgcatcg acgtcccgga agggggctcc
     2161 ggtggcggtc aagagagcga taacctagag gaggcaacgc gatgagcgcg aacccgcagg
     2221 atggatcccg tccgacagtc gtcctcgtgc acggcgcttt tgctgatggt tcgagctgga
     2281 acgaagtgat cgagcggctg caggcagagg gagtacacgt tacggccccc gccaatccac
     2341 tacgcggaat tgcaatcgac tccgcctaca tcaacagctt tttcgaacag atcccaggcc
     2401 cggtcctcgc cgtcgcccat tcctacggcg gtgcggtgat caccaacgcc gccaccaacg
     2461 ccaacaacgt cctcgggctg gtctatgttg ccgcattcgc ccccgacgaa ggcgaaaccc
     2521 tgggtgctat tgcaagcaac tccacagaca gcgtggtact cccagccttg gtcgagctcc
     2581 agtacccgac cggagagg
//
LOCUS       DASTUV010000405         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1102180, whole genome shotgun sequence.
ACCESSION   DASTUV010000405 DASTUV010000000
VERSION     DASTUV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102180"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1530)
                     /locus_tag="VFT03_08190"
     CDS             complement(106..1530)
                     /locus_tag="VFT03_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4848164.1"
                     /translation="MAEASADRRGGREQGHLQPGLKRRHMTMIAIGGIIGAGLFVGTG
                     PILNQAGPATILTYLLTGTILILVMRMLGEMAVAQPSVGSFSDYSRMALGNWAGFSVG
                     WLYWYFWAIVVGFEATVAAGILGEYIPGIPPWLISLFLILLLTATNLYSVGSYGEFEF
                     WFAGIKVAAIIIFIVLAILFVTGVLPGGGMGLSNLYAHGGFFPNGGLVMFSGVVTVIF
                     AFVGAEIVTIAASESNEPERGVARATNQVIYRILLFYVGSVFFLAAVVPWDTKFSADA
                     VLQSPFTIAFQKMGIPFAPDIMNFVVLTAVLSVLNSSLYTTSRMLFALTRHNDAPGFF
                     TDTTRRGVPMWAILAGTSFAYISVVIYYFFPEEIFTWLINASGAIALFVYLLIAISEL
                     VMRRRLERESPDRLQLRMWLYPWLTYLSIAAMVAVLIAMAVIADQRPLLIASLISAAV
                     ILVAYLLRRQFGPPEKDPREVAQR"
     gene            complement(1650..3029)
                     /locus_tag="VFT03_08195"
     CDS             complement(1650..3029)
                     /locus_tag="VFT03_08195"
                     /inference="COORDINATES: protein motif:HMM:NF005812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU4848165.1"
                     /translation="METKEDARIRELAEKDERFVWHALSRYTPVEGGGTAPAPRIIVT
                     EGKGAWISDAQGNRYLDGMSGLWCVNVGYGREELAKAAYEQLVKLPYYPLTMGHEPAV
                     ELGERLNEWLGDEYVIFYSNSGSEANEVAFKVARQYHEQNGEPRRWKFVSRYRAYHGN
                     SMGSLAATGQALRKYRYEPLASGFLHVAPPDRYRCAYCADRPACNMECARAIERTIHW
                     ELPETVAGVIMEPIITGGGVLVPPDGYVEEVARICRKNGVLLIVDEVICGFGRTGRRF
                     GYQHYGVKPDIVTMAKGITSAYLPLSATAVKRDIFEAFKGTEEYDHFRHVNTFGGNPA
                     ACALAVKNLEIMEEEDLSARSAKMGERLRGKLAGLEDHPNVGEIRHKGLLFGIELVED
                     RETRTPATAERVAKVISGCKERGLIVGKNGDTVAGFNNVITLAPPLVVSDDDLEFISG
                     ALSESLRSL"
BASE COUNT          586 a         1020 c          982 g          556 t
ORIGIN      
        1 gtgcgcacgc ccgagaagcg cggcgtcacg aacgagtcgg ccggctcgta gcgggtcaag
       61 ggaccctctc ctttcgtctg ggagcgcgcg gggcgttctc tttctctatc tctgggccac
      121 ctccctggga tctttctccg ggggtccgaa ctggcggcgt agcaggtagg caaccaggat
      181 cacggcggcg ctgatcaagc tcgctatcag caggggtcgc tggtcagcga tgaccgccat
      241 cgcgatgagc acggccacca tggctgctat agacaggtac gtcagccacg ggtagagcca
      301 catcctcaac tggagcctgt cgggtgactc gcgctcgagg cggcggcgca tcacgagctc
      361 agagatggcg atcagaaggt agacgaagag cgcgatggcg cccgaggcgt tgatcagcca
      421 agtgaagatc tcctcgggga agaaatagta gatcacgacg gagatgtaag cgaaactggt
      481 ccctgcgagg atcgcccaca tcgggacgcc gcggcgggtg gtatcggtga agaaccccgg
      541 cgcgtcgttg tgcctcgtga gcgcgaagag catccgggag gtcgtgtaga ggctcgagtt
      601 gagaacgctg agcacggcgg tcaggaccac gaagttcatg atatcgggcg cgaagggtat
      661 ccccatcttc tggaaagcga tggtaaacgg gctctggagt accgcgtcgg cggagaactt
      721 ggtgtcccac ggcaccaccg ccgccaggaa gaagaccgag cccacgtaga agagcagaat
      781 cctgtagatg acctggttgg tcgcccttgc tacgccccgc tcgggctcgt tggactcgga
      841 agccgcgata gtgacgatct ccgccccgac gaacgcgaag atcaccgtga cgacgccgct
      901 gaacatcacc agacccccgt tggggaagaa gccgccgtga gcgtagaggt tcgagagacc
      961 cataccacca cccggcagca cgccggtcac gaacaggatg gccagcacga tgaagatgat
     1021 gatcgccgcc acctttatac cggcgaacca gaactcgaac tcgccgtagg agccgaccga
     1081 gtagaggttg gtggcggtta gcagcaggat caggaacaag gatatgagcc acggcggtat
     1141 ccccggtatg tactcaccca ggatgccggc cgccacggtg gcctcgaagc cgaccactat
     1201 cgcccagaag taccagtaga gccacccgac ggagaaaccg gcccagtttc ccagagccat
     1261 gcggctgtag tcggagaagg agccgaccga gggctgcgcc accgccatct cgccgagcat
     1321 ccgcatgacc aagatgagga tggttcccgt cagcaggtac gtaaggatcg tcgccggacc
     1381 cgcctggttg aggatcggtc cggtccccac gaagaggccc gcgccgatga tcccgccgat
     1441 ggcgatcatg gtcatgtgcc gcctctttaa accaggctgc agatgaccct gctcgcgtcc
     1501 accccgccgg tccgcgctgg cttcagccat gcctgcccct ttcaaccgcc tcgatcccct
     1561 gtcagggcag cttacggcca cccacgttcc cccgcaaccc gtacggcgaa caataagtcg
     1621 agacgacccg ggcgtgccgg ccgtgctctc tacaaactac gcaggctctc gctcaaggcg
     1681 ccggagatga actccaggtc atcgtcgctc acgaccagcg gaggggcgag ggtgatgacg
     1741 ttgttgaagc ctgccaccgt gtcgccgttc ttccctacga ttagccctct ctccttgcag
     1801 ccggagatga ccttcgcgac tctctcggcc gtggccgggg tccgggtctc tctgtcttcg
     1861 acgagctcta ttccgaagag gaggcccttg tgcctgatct cccctacgtt cggatggtct
     1921 tcgagtccgg cgagcttgcc acgcaacctc tcacccatct ttgccgagcg cgcggacagg
     1981 tcctcctcct ccatgatctc gaggttcttc accgcgagcg cgcacgccgc cggattgccg
     2041 ccgaaggtgt tgacgtggcg gaaatgatcg tactcctccg tccccttgaa cgcctcgaag
     2101 atatcgcgct tcacggcggt ggcggagagc ggcaggtagg cgctcgtgat gcccttggcc
     2161 atcgtcacta tgtccggctt gacgccgtag tgctggtagc cgaaacgccg gcccgtgcgg
     2221 ccgaaaccgc agatcacctc gtccacgatg agcaggacgc cgttcttccg gcaaatcctt
     2281 gccacttcct cgacgtagcc gtcgggcggg acgagcacgc cgccacccgt gatgatgggc
     2341 tccatgataa ccccggcgac cgtctcgggc agctcccaat gaatggtgcg ctctatggcc
     2401 ctggcgcact ccatattgca cgcaggccgg tcggcgcagt aagcgcagcg gtagcgatca
     2461 gggggcgcga cgtgcaggaa gccggaagcg agtggctcgt agcggtactt gcgcagagcc
     2521 tggcccgtgg ccgccaacga gcccatggag ttgccgtggt atgcccggta gcgggagaca
     2581 aacttccacc gcctcggctc gccgttctgc tcgtggtact ggcgggctac cttgaaggct
     2641 acctcgttcg cctcagaccc cgagttggag tagaagatga cgtactcgtc accgagccac
     2701 tcgttgagtc tctcgccgag ctccacggcg ggctcatggc ccatcgtgag cggatagtag
     2761 ggtagtttga cgagctgctc gtaggcagcc ttcgccagct cttcgcgtcc gtagcccacg
     2821 ttgacgcacc acagaccgga cataccgtcg agataacggt tgccttgggc atcggagatc
     2881 caggccccct tcccctccgt cacgattatc cgcggggccg gcgctgtgcc tcccccctcg
     2941 accggcgtgt agcgcgacag ggcgtgccag acgaaacgct cgtccttctc ggccagctcg
     3001 cggattctcg catcctcctt ggtctccatg ccagtctcct acctgagtag cttctctggg
     3061 tcggtgtagc gcaggtcgtg ggcctctgct acgggctccg agacgaggct accgtccagc
     3121 gtggagagac ccttcgccag cgta
//
LOCUS       DASTUV010000406         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_832269, whole genome shotgun sequence.
ACCESSION   DASTUV010000406 DASTUV010000000
VERSION     DASTUV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832269"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFT03_08200"
     CDS             <1..491
                     /locus_tag="VFT03_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU4848166.1"
                     /translation="FTRAHTHHLPDEHVHKHALGPFVLGLAVHNLADGFVLGVGAKAG
                     AVTSWLVGLGVLVHQIPVGISLAAVLLAAQVGRAGVMRTAVLLGLAIPAAAALTVAFP
                     VPGEGTLGLLTGVAGGVLAYIGAAHLLPEAQTEDTNRVAGILFVATLLITAVGLMLLG
                     HG"
     gene            complement(527..1219)
                     /locus_tag="VFT03_08205"
     CDS             complement(527..1219)
                     /locus_tag="VFT03_08205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4848167.1"
                     /translation="MNDLSRLPVTFGLIATCVAVYVAVALAGQGYGYPLNFGLVTQPP
                     EVLAQGALVPAAVAEGRVWLLLSSMFLHSGFIHLALNMLSLYFLGSFVEQAFGKSRFL
                     ALYLSSGLAGGIAYLYFGAFDRPAVGASGAIFGLLGGVLGYSVRRGTFSWQNPLIRQL
                     LILTALNLYFGFSVPNISNTAHIGGLVGGAIFGWLTAPTVSSSKTLRAATPTVIVFGA
                     ELILLALWLLWK"
     gene            complement(1241..1687)
                     /locus_tag="VFT03_08210"
     CDS             complement(1241..1687)
                     /locus_tag="VFT03_08210"
                     /inference="COORDINATES: protein motif:HMM:NF027852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HEU4848168.1"
                     /translation="MAVEGKVAKILGNNEIVINRGRGEGVRQGMLFEIFAPEGEEVWD
                     PDTGETLGTVEDVKAKAEVTEVKERLAVARLQNAQDTPFGAVNIGEMQENLQRMFGQM
                     FGEGVQIRGFGASSGDDTDFESMLGGPLQDLSQVQVGDAVREIKRR"
     gene            1776..>2312
                     /locus_tag="VFT03_08215"
     CDS             1776..>2312
                     /locus_tag="VFT03_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU4848169.1"
                     /translation="MPERTNRIGRHLPTSGGLARTLWAARELGCETIQIFVSNPQAWT
                     EPRQHQDADIFVEGSRELGISPVVAHAKYLINLASTSKEQWELSALALAHELGAAGAL
                     GVDLVVVHAGSHGGDGEEKGMERLVEGLGRAREQAAILDGGAGVVAEPVVENSVGAGT
                     QLCSTFEELREVALRGGVR"
BASE COUNT          421 a          723 c          759 g          409 t
ORIGIN      
        1 cgttcaccag ggcccacacg caccacctac ccgacgagca cgtccacaaa cacgcgctcg
       61 ggcctttcgt cctaggattg gccgtccaca acctcgccga cggattcgtt ctcggcgtag
      121 gagccaaggc tggagccgtc acctcctggc tcgtagggct cggggttctc gttcaccaga
      181 tacccgttgg gatctccctc gccgccgtcc tgcttgctgc gcaggtcgga agggcagggg
      241 tcatgaggac cgcggtcttg ctcgggctgg cgataccggc cgcagcggcg ttgaccgtgg
      301 ccttccccgt gccaggagaa ggcactctcg gtctgctgac cggcgtcgca gggggggtgc
      361 tcgcatacat cggcgccgca cacctgctcc ccgaggccca aaccgaggat acgaacaggg
      421 ttgccggcat cctgttcgtg gccacgctcc tcatcaccgc cgtcgggcta atgttgctcg
      481 ggcacggtta gagcggttgg cttgtaggga cggtttgcga gccgccctac ttccagagta
      541 gccagagtgc gagcaggatc agttccgcac cgaacacgat gacggtcggt gtcgccgcgc
      601 ggagtgtctt gctggaggag acggtgggcg ccgtgagcca tccgaatatg gctccgccga
      661 cgagaccgcc gatatgcgcc gtgttgctta tattggggac ggagaatccg aagtagaggt
      721 tcagggcggt cagtatcagg agctggcgga tcagtggatt ctgccaggag aaggtgccgc
      781 ggcgcactga gtaaccgagc actcctccga ggagaccgaa gatcgcgccg gaagctccta
      841 cggcgggcct gtcgaaggcg ccgaagtaga ggtaggcgat gcccccggcg agcccgctcg
      901 acaggtagag cgctaagaaa cggctctttc cgaatgcttg ctcgacgaac gagccgagga
      961 agtacagcga gagcatgttc agcgccaggt ggatgaaccc cgagtgcagg aacatgctcg
     1021 acagcaagag ccacactctg ccttcggcga cggcggctgg cacgagggcg ccctgcgcga
     1081 gcacctcggg gggctgtgtc accagcccga agttcagcgg gtaaccgtag ccctggcccg
     1141 cgagcgcgac ggcgacgtat acggccacac aggtggctat gaggccgaac gtcaccggta
     1201 ggcgggagag gtcgttcaat gccgggtact tacgtcgggt ctagcggcgt ttgatctcgc
     1261 gcacggcgtc cccgacttgt acctgtgaaa gatcctgcag gggcccgccg agcattgact
     1321 cgaagtcggt gtcgtcgcca gacgaagcgc cgaagccgcg gatctggacg ccttcgccga
     1381 acatctgccc gaacatacgc tgcaggttct cctgcatctc cccgatgttg actgctccga
     1441 acggcgtatc ctgtgcgttc tgtagacggg caacggcgag tctttccttg acctctgtaa
     1501 cctcggcctt cgctttaacg tcctcgacgg tgccgagcgt ctcgcccgta tcagggtccc
     1561 aaacctcctc gccctcggga gcgaagatct cgaagagcat accctgacgg acgccctctc
     1621 ctcttccgcg gtttatgacg atctcattgt ttcccagaat cttcgctacc ttgccttcga
     1681 ccgccatgcg gttggctcct tccgatcata ttgatggtct cacgagatta tatcgttcct
     1741 ggcgagggcc gccgcaggac ggtagaatcc tcgccatgcc agagaggacg aacagaatag
     1801 gacggcacct tccgacctcc ggcgggctcg ccaggacact ttgggcggca cgcgagctcg
     1861 ggtgcgagac tatccagatc ttcgtctcca acccgcaagc ctggactgaa cccaggcaac
     1921 accaggatgc cgatatcttc gtcgagggaa gccgcgaact gggcatctcg ccggtcgtcg
     1981 cacacgcgaa gtaccttata aaccttgcct ccacgagcaa ggagcagtgg gaactttcgg
     2041 cgctggccct ggcccacgag ctgggcgccg caggcgcgct cggggtcgac ctggtcgtcg
     2101 tgcacgctgg cagccacgga ggagacggcg aggagaaggg catggagcgg ctcgtggaag
     2161 gcctggggcg tgcgagggag caggcagcca tactcgacgg aggcgcagga gtcgtggccg
     2221 aacccgtggt cgagaattcc gtcggggccg gtacccagct ctgctctacg ttcgaggaac
     2281 tccgggaggt cgcgctgcgc ggcggggtga gg
//
LOCUS       DASTUV010000407         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_295141, whole genome shotgun sequence.
ACCESSION   DASTUV010000407 DASTUV010000000
VERSION     DASTUV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295141"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFT03_08220"
     CDS             complement(<1..1062)
                     /locus_tag="VFT03_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEU4848170.1"
                     /translation="MTDPRVTGAVRYSQDVAIEGMLHACFVRSPVAHGRVVGVDAPSL
                     PEGIIVLLPQDVRDLGSYGPQIKDQEILPQERVRYAGDVVAAVAAETLEEAEEAVGLI
                     DVEYEELPAVFDEVESASEEAPLVHETINISKNDAAYFGIRPQQGTNICHRFRLRHGD
                     VEAGFVEADAVVEETYHTAGATHAPMEPHAAVARWDGDRLEVTTGTQTPFNMRTDLAG
                     LFGIDEEKVRIISPPMGGSFGAKTFVRTEAIAACLARKAKRPVKIVLERDEMWMTLNR
                     HPATVRVKLGARSDGTLVASETECWANTGAYADCGPGVAQKMGFAAPGPYRIANVRVD
                     SRAVYTNLPPNGAFRGYGQM"
     gene            complement(1055..1888)
                     /locus_tag="VFT03_08225"
     CDS             complement(1055..1888)
                     /locus_tag="VFT03_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU4848171.1"
                     /translation="MSAPEWHEPRSLDEALSLRAEHGEEATVVAGGSFLGIVINQKLF
                     APKRLVALRSVPQLDYIEADGEKLMLGAMTTHRAVERSPVVKEAWPSLAFAFSVVASP
                     RVRNQATVGGVVADADYASDPPSMLAALEARVVARSVRGEREIPMDEFVLDHYETALE
                     VDELVTELRIPRSEGTSVYRKFRSRSSEDRPCVSVAAAQEYGALRVVVGAVAGRPQYF
                     PEICDLARGERLSDELATEIGHRYAEEIEPLSDSRGSARYRRRVIAVEVRRALEDLHH
                     D"
     gene            complement(1931..>2746)
                     /locus_tag="VFT03_08230"
     CDS             complement(1931..>2746)
                     /locus_tag="VFT03_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU4848172.1"
                     /translation="REVEVAGGKGASLARMTAEGLPVPSGFVIPSYVLQNSIDAERML
                     KLAASRSTRELQEVVAATEPPWEKITAAYENLVGGRLTSTYKLVGNKKVAVRSSAVAE
                     DSEAASYAGQQESYLFVEGAEEVCRRVVDCWASFFSERALFYRSQKGSLEDLRMAVVV
                     QKMVDPEKSGVIFTVDPVRRRRDRMVVEAVRGIGEQVVSGEVTPDHYTLDRRGKVKRE
                     KIVDDRVLTDEELQKLGDLGRQLEERHGVAQDIEWAMVGEEIYLLQSRPVTTM"
BASE COUNT          453 a          920 c          879 g          494 t
ORIGIN      
        1 catctggccg tagccgcgga aggcgccatt gggcggaagg ttggtgtaga cggcacgtga
       61 gtcgacccga acgttggcga tgcggtacgg cccaggggcg gcgaagccca tcttctgggc
      121 gacgccggga ccgcagtcgg cgtaggcccc agtgttggcc cagcactcgg tctcgctagc
      181 gacgagtgtt ccgtcgcttc gagcaccgag cttcacccgg accgttgccg ggtggcggtt
      241 aagcgtcatc cacatctcat cgcgctcgag aacgatcttg accggcctct tcgccttgcg
      301 agcgaggcac gcggctatcg cctccgtcct gacgaaggtc ttcgccccga acgaacctcc
      361 catagggggt gagattatcc tgaccttctc ctcgtctatg ccgaagagcc cggcgaggtc
      421 cgtgcgcatg ttgaacgggg tctgcgtacc ggtcgtgacc tccaaccggt ccccgtccca
      481 gcgggcaacg gcggcgtggg gctccatcgg ggcgtgcgtc gccccggcgg tatggtaagt
      541 ctcctcgacc acggcgtcgg cctccacgaa cccggcctcg acgtcgccgt ggcggagccg
      601 gaagcggtgg cagatgttcg ttccctgctg tggtcgaatg ccgaagtacg cagcgtcgtt
      661 cttagagatg tttatcgtct cgtggaccag cggcgcctcc tctgatgcgg actctacctc
      721 atcgaagacg gcgggaagct cctcgtactc aacgtctatg agccccaccg cctcctcggc
      781 ctcctcaagc gtctcggcgg cgacggccgc gactacgtcc ccggcgtagc gaactcgctc
      841 ctgagggagg atctcctggt cctttatctg cggcccgtag ctgccgagat cccggacgtc
      901 ctgcggtagg aggacaataa tgccctccgg gagggacggg gcgtccaccc ccaccacgcg
      961 accgtgggcc acgggagagc gcacgaagca ggcgtgaagc atcccctcta tcgccacgtc
     1021 ttgcgagtag cggacggctc cggtaacgcg gggatcagtc atggtgcaga tcctccaggg
     1081 cacgcctgac ctccacggcg atgacgcggc gacggtatcg agccgagccg cgtgagtcgg
     1141 agagcggctc aatctcctcg gcgtagcggt gtccgatttc ggtggcgagc tcgtcgctca
     1201 ggcgctctcc cctagccagg tcacagatct ccgggaagta ctggggccgt ccggcgaccg
     1261 cgccgaccac gacccgtagc gcgccgtact cctgggccgc cgcaacagag acgcagggcc
     1321 gatcctcgct tgagcgcgag cggaacttcc ggtagacgga ggttccttcg ctgcggggaa
     1381 tccggagctc tgtgacgagc tcgtccactt cgagtgcggt ctcgtagtgg tcgaggacga
     1441 actcgtccat cggtatctcg cgctctcccc tcacgctccg cgcgacgacc cgtgcctcaa
     1501 gggcggcaag catcgagggg ggatcggagg cgtagtccgc gtcggcgacg accccgccca
     1561 cggttgcctg gttcctgacg cgcgggctgg cgacgaccga gaaggcgaag gccagcgaag
     1621 gccaggcctc cttcactacg ggcgagcgct cgacggcccg gtgggtggtc atcgccccga
     1681 gcattaactt ttcgccatcg gcctcgatat agtcgagttg tgggacgctg cggagggcga
     1741 cgagcctctt gggggcgaag agcttctggt taatgacgat cccgaggaag gagccgcccg
     1801 ccacgacggt cgcctcttcc ccgtgttcag cacgcaggga gagagcttcg tccagcgagc
     1861 gtggctcgtg ccactccggt gcgctcattg ccatctcaaa tgtccttcca aaccgcgtca
     1921 aatagtacgc ctacatcgtc gtgacaggcc gggactggag gaggtagatc tcctcgccaa
     1981 ccattgccca ctcgatgtcc tgtgcgacgc catgacgttc ctctagttgc ctgccgaggt
     2041 cgccgagctt ctgcagctcc tcgtcggtaa ggacgcggtc atctacgatc ttctcgcgct
     2101 tgacttttcc cctgcggtcg agggtgtagt ggtcaggggt cacctcgccg gagacgactt
     2161 gctccccgat gcccctgacg gcctcgacaa ccatccggtc gcggcgacgc ctgacagggt
     2221 ctacggtgaa gatcacgccg gacttctcgg gatccaccat cttctgcacg acgactgcca
     2281 tccgcagatc ttcgagggag cccttctgcg aacgatagaa gagcgcgcgc tcggagaaga
     2341 aagacgccca gcagtccacg acccgcctgc agacttcctc cgcaccttcg acgaagaggt
     2401 agctctcctg ctgcccggcg tagctcgccg cctcagagtc ctccgcgacg gcggaggaac
     2461 gaacggccac cttcttgtta cccacaagct tgtacgtgga cgtgagccgc ccgcccacga
     2521 ggttctcgta ggcggccgtt atcttctccc acggaggttc ggtagccgcg acgacctctt
     2581 ggagctctct tgtggagcgg gaggccgcga gcttcagcat ccgctcggcg tcgatggaat
     2641 tctgaagaac atacgagggg atgacgaagc ccgagggtac cgggagtccc tcggcggtca
     2701 tgcgggccag gctggcgccc ttcccaccgg ccacctccac ctcccg
//
LOCUS       DASTUV010000408         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1261355, whole genome shotgun sequence.
ACCESSION   DASTUV010000408 DASTUV010000000
VERSION     DASTUV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261355"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..>2547
                     /locus_tag="VFT03_08235"
     CDS             45..>2547
                     /locus_tag="VFT03_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848173.1"
                     /translation="MPVVEGAGDELREEGVEAAFDLTLPTTLPYGEKNRHSTDDVTFR
                     AAYGIEAGVLAALANLDPTLPTRLAGFEGSRALSVRAQVGPWTAHLAGTVTVRALKAE
                     GGYLQAEEYQRLLEEAGDVRVIPLPEENGAVRTLLDGTYLQNIVFSKLRSIHSYRPAA
                     VVAPLTGESFQVFFVAPQISYGVVGRLRAVLAASGLAGDERDTLRRLADEALDIPRID
                     ARVRHSFLRGEGPPHMEDRPGLPRDSGFDPIASLLDAAGEISPRSAAPTTEELFEESG
                     WGAVETEELDGFRRHRSEESCVVALGEHDTAILEVRESDEGHTLHTAPVAGLDPEDPK
                     GRAVYMTTLLEYLHQTVLSSNRIVRVYDRHADELKRLLAARMLEFNPDAQVPGRATAP
                     IEIGSLRGTRYRFGSERVPSGDRVVQVDLYKRDDGSEEPRSVTLALGTGVEDPIVLMP
                     TTVQLAFGFGNICGDLGVLERRLAYRVVAFNRSPNERALNALENGVPLYEFDETLDEQ
                     GETVAEDRQRRAYERAGLKLSGKLRDLLDEGLVVGETDGGVPVRARVGHILDGLLGSI
                     EHPVTGEKTRISEYYKELLFDEYEGRGVPTIYNGSNSPEKVADGRIFIHDAFTTYGLE
                     PAEHYDEHDGPNQSLMCVSCNTTNITGILLRLTDVPGISGMKIRVMTHRKANDQYVGE
                     GKQSVNYQGMAFDFHYHHWDDARLFYDRIKDESVRRRLGAMFALDNVREGDSAAWAIS
                     THATGQAATEFHTGVVHLSGELHGKPLDAEELKTELSRRESESAQLIEFPGKVDARHL
                     LETLHYRIGIRNLYVQPFTVTQDGDDLAIVFFTPHL"
BASE COUNT          458 a          839 c          844 g          406 t
ORIGIN      
        1 cttcactgct ccccctcccg gaggccgcag ttcaggagtt cttgttgccc gttgtagaag
       61 gagcgggcga cgagcttcgg gaggagggcg tcgaggcggc gttcgacctc accctgccca
      121 cgaccttgcc gtacggcgag aagaaccgtc actccacgga cgatgttacc ttcagggcag
      181 cctacgggat cgaggctggg gtcctcgccg ccctcgcgaa cctggatccc acgctcccga
      241 cccggctcgc gggcttcgaa ggctcgcggg ccttgagcgt gcgggcccag gtcggcccct
      301 ggacggccca cctcgccggg accgtgaccg tccgggcgct caaggcggag ggcggatacc
      361 tgcaagccga ggagtaccag cgactcctgg aggaggcggg agacgtcagg gtcatccctc
      421 tgccggagga gaacggcgcc gtccggacgc tgcttgacgg gacgtacttg cagaacatcg
      481 tcttctccaa gctccgttcc atccacagct accgcccggc cgcggtcgtc gcgcctctca
      541 ccggcgagtc cttccaggtg ttcttcgtcg cgccccagat atcctacggc gtcgtgggcc
      601 ggttgcgggc cgtgctcgcc gcctcgggcc tggccggcga tgagcgggac acactcagga
      661 ggctggccga cgaagccctc gacatacctc ggatcgatgc cagggtccgc cactccttcc
      721 tgaggggcga gggaccgccg cacatggagg accggccggg gttgccgcgg gactctggct
      781 tcgaccccat agcctctctg ctcgatgcgg cgggggagat ctcacctcga tccgcggctc
      841 ccactacgga ggagctcttc gaggagtccg gatggggtgc ggtggagacc gaggagctgg
      901 acgggtttcg tcgccaccgc tccgaggaga gctgcgtggt ggctctcggg gagcacgata
      961 ccgccatcct cgaggtgcgg gagtctgatg agggtcacac cctccacacg gcgcccgtcg
     1021 cgggcctgga cccggaggat ccgaagggca gggccgtgta catgaccaca ctcctcgagt
     1081 atctgcacca gacggttctg tccagtaacc gcatcgtgcg cgtatacgac cggcacgctg
     1141 acgagctcaa gcgcctcctt gctgcccgca tgttggagtt caacccggac gcgcaagttc
     1201 cgggcagggc cacggctccc atagagatag gctcgttgcg gggaacccgt taccggttcg
     1261 gtagcgagcg ggtcccgtcc ggggaccggg tggtccaggt ggacctctac aagcgcgacg
     1321 acgggtccga ggagccccgg agcgtgacgc tcgccctcgg tacaggggtg gaggacccca
     1381 tagtgctcat gccgaccacg gtccagctcg cctttggttt cggcaacatc tgcggggatc
     1441 tgggtgtcct ggagcggcgg ctcgcctaca gggtcgtcgc cttcaaccgc tcgccgaacg
     1501 agcgagccct gaacgccctg gagaacggcg tccccctcta cgagttcgac gagaccctcg
     1561 acgagcaggg tgaaaccgtc gccgaagatc ggcagcggag agcctacgag cgggccgggc
     1621 tcaagctcag tgggaagctg cgggatctcc tggacgaggg actcgtcgtc ggcgagacgg
     1681 atgggggcgt cccggtcagg gcgcgcgtag gacacattct cgacgggcta ttggggagca
     1741 tcgaacaccc ggtcacgggc gagaagacca ggatcagcga atactacaaa gagctgctct
     1801 tcgacgagta cgaggggcgc ggcgttccga ccatctataa cgggtccaac tcgccagaga
     1861 aggtcgctga cggcaggatc ttcatccacg atgccttcac gacgtatggg ctggaaccgg
     1921 cggaacacta cgacgagcac gacgggccaa accagagtct catgtgcgtc tcgtgcaaca
     1981 cgaccaacat cacggggatc ctgttgcgcc tcaccgacgt gccggggata tcgggcatga
     2041 agatccgggt catgacccac cgcaaggcga acgatcagta cgttggagag ggcaagcaga
     2101 gcgtcaacta ccagggtatg gccttcgact tccactacca ccactgggac gacgccaggc
     2161 ttttctacga ccggatcaag gacgaaagcg tccggcggcg gctgggcgcc atgttcgccc
     2221 tcgacaacgt ccgcgaggga gattcagcgg cttgggccat ctcgacccac gccacggggc
     2281 aggcggccac cgagttccac acgggcgttg tgcacctgag tggcgagctg cacggcaaac
     2341 ctctagacgc cgaagagctc aagaccgagc tgagcaggcg cgagtccgag agcgcccagc
     2401 tcatcgagtt ccccggcaag gtggacgccc ggcacctgct cgagacgctc cactaccgca
     2461 tcggcatccg caacctctac gtccagccct ttaccgttac ccaggacggg gacgacctgg
     2521 cgatagtctt cttcactcct cacctct
//
LOCUS       DASTUV010000409         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_260273, whole genome shotgun sequence.
ACCESSION   DASTUV010000409 DASTUV010000000
VERSION     DASTUV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260273"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFT03_08240"
     CDS             complement(<1..384)
                     /locus_tag="VFT03_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848174.1"
                     /translation="MGGVLLRACAKVNYALEVRGLRGDGYHEISSVLQSVSLADELEI
                     ERSRGGFELSFEPEGVEIGPVEENTVYKAWALLWEASGHELPTRIRLHKKIPAGAGLG
                     GGSADAAAVLVGMNELFDLGLDAEQL"
     gene            complement(384..1172)
                     /gene="rsmA"
                     /locus_tag="VFT03_08245"
     CDS             complement(384..1172)
                     /gene="rsmA"
                     /locus_tag="VFT03_08245"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU4848175.1"
                     /translation="MSSPANPPKKRLGQHFLKDPNTARIVAEGVTEDDIVLEVGPGRG
                     FLTAVLAQRARLVYAVELDPDVLPDLRRAVRGFDNVVIHEGDAVRFDYRTLDPAPNKL
                     VANLPYNVASPLVLRLLEEVRSLDSLRFMVQLEVARRMAASPGTKDYGAYAVLVQLLA
                     ESRIVHRVSPQVFDPPPRVYSAVVEMRRRRPGLEPAEYRAVKELVLAAFKSRRKRLVN
                     NLPGDLREGATRALISLGFGPDARAEELSPEDIVALRRMLAEAI"
     gene            complement(1182..1940)
                     /locus_tag="VFT03_08250"
     CDS             complement(1182..1940)
                     /locus_tag="VFT03_08250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU4848176.1"
                     /translation="MLVDSHTHLLRLDVSPEEAVREAAEAGVGAVVNIGTDVEDSQRG
                     AELAAVLPNVYTAAGIHPHNAGAYTAASLEALAELSESPGVVALGEVGLDYYRGYATP
                     EAQKALFEDVISLANDTRLPLVIHSREAFPDTMALLGSARVPVVLHCFEGGEREVNEA
                     RERDYYVGLAGNVTYKNSKTAEVLRLVDQDRLLVETDAPYLSPHPVRGERNVPRNVVH
                     TARFVAERLGMEYEELATLTTRNARNLFGLPLEV"
     gene            complement(2006..2251)
                     /locus_tag="VFT03_08255"
     CDS             complement(2006..2251)
                     /locus_tag="VFT03_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848177.1"
                     /translation="MPASGKSPEEWIGQQVLVEVLGGRVYQVIVRLEGINDWGVVLSG
                     EGVDPGGGSVFFPWRHVMAMRPARPGDEPPDYRGARE"
     gene            complement(2265..3071)
                     /gene="rsmI"
                     /locus_tag="VFT03_08260"
     CDS             complement(2265..3071)
                     /gene="rsmI"
                     /locus_tag="VFT03_08260"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU4848178.1"
                     /translation="MVLVVVPTPIGNLEDITLRALRVLSEADLVACEDTRRTGRLLAH
                     HGIKNDLVSYHEHNEERLTPELAERARTQEIALASDAGMPLVSDPGYRLVTACIEKGV
                     DVEVLPGPSAPITSLVASGLPADVVVFLGFLPRKGRERTELLARISKERSTFILFESP
                     HRLGKTLGDLPPETLVAVCRELTKLHEEVFRGTAAEAARRFSEGTKGEIVLVVRGGTA
                     AGPGSVEQAVELARGYVEEGESPSRAAARAARESGRKKGEIYARLVEAWP"
     gene            complement(3075..>3470)
                     /locus_tag="VFT03_08265"
     CDS             complement(3075..>3470)
                     /locus_tag="VFT03_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4848179.1"
                     /translation="RARELASEHPGSALGRLAADREVLGEDVTRLAREGDGVAVAVLE
                     EAGRWLGIGLAGFVNVFNPEVITVGGGVMAAGELILEAAREEVRLRARPPSRDLVQIK
                     EATLGPMSGVLGAAALARDPSSGEYVLES"
BASE COUNT          569 a         1221 c         1112 g          568 t
ORIGIN      
        1 cagttgctcg gcgtccagcc ccaggtcgaa aagctcgttc atgcctacca gaaccgccgc
       61 cgcgtccgcg gatccgccac ccagaccagc ccccgcgggt atcttcttgt gaagccgtat
      121 cctagtcggg agctcgtggc cggaggcctc ccagagcaac gcccaggcct tgtagacggt
      181 gttctcctcg acgggcccga tctccacgcc ctccggctcg aaggacagct cgaagcctcc
      241 cctggagcgc tcgatctcga gctcgtcggc gagcgagacg ctctgtaaca cgctcgagat
      301 ctcgtggtac ccgtcccccc gcaagccccg cacctcgagg gcgtagttca ccttggcgca
      361 ggctcgcagt agcacacccc ccatcagatc gcctccgcga gcatccggcg cagcgcgacg
      421 atgtcctccg gcgagagctc ttccgcccgc gcgtcgggcc cgaaaccaag agagatgagg
      481 gcgcgagtcg cgccttccct caggtcgcca ggcaggttgt ttaccagccg cttgcggcgg
      541 ctcttgaacg cggcgagcac gagctccttg accgcccggt actcggcggg ctcaagaccc
      601 ggtcttcgac gtcgcatctc cacgacggcg gagtataccc gcggcggcgg gtcgaagact
      661 tgcggcgaga ccctgtggac gatcctcgat tccgcgagga gctggaccag gacggcgtaa
      721 gcaccatagt ccttggttcc cggcgaggcc gccatgcgcc gcgctacctc gagttgcacc
      781 atgaaacgca gcgagtcgag gctccgcacc tcctccagca gcctcaggac gagaggcgag
      841 gcaacgttgt agggcaggtt ggctacgagc ttgttgggcg cgggatccag agtcctgtag
      901 tcgaagcgga cagcgtcgcc ctcgtggatg acgacgttgt caaagcctcg cacggcccgg
      961 cgcaggtcgg gcagcacgtc tgggtcgagc tcgacggcgt acaccagcct cgctctttgc
     1021 gcgaggacgg cggtgaggaa gccccgcccc ggtccgacct cgaggactat gtcatcctct
     1081 gtgacacctt ctgcaactat gcgggcggtg ttagggtcct tgagaaagtg ctggccgaga
     1141 cgtttcttcg gggggttcgc aggggaggac aacaaagagc ttcagacctc gagtggcaag
     1201 ccgaacaggt tgcgagcgtt gcgggtggtc agggtcgcga gctcctcgta ctccattccg
     1261 agccgctcgg cgacgaagcg ggcggtgtgg accacgttcc tcggcacgtt cctctcaccg
     1321 cgcacgggat gtgggctcag gtagggcgcg tccgtctcga cgaggagcct gtcttggtcc
     1381 acgaggcgga gaacctccgc ggtcttgctg ttcttgtacg ttacgttgcc ggcgaggccg
     1441 acgtagtagt cgcgttctct cgcttcgtta acctcgcgct caccgccctc gaagcagtgg
     1501 aggacgacgg ggactcgggc gctgccgagc agggccatcg tgtcggggaa cgcctcgcgg
     1561 gagtggatta ccagtggcag acgggtgtcg ttggcgaggg agattacatc ctcgaagagc
     1621 gccttctgcg cctcgggcgt cgcgtatccc cggtagtagt cgagcccgac ctctcccaga
     1681 gctactacgc caggcgactc agagagctcc gcgagcgctt cgagactggc cgccgtgtag
     1741 gctccagcgt tgtgggggtg tatgcccgcc gccgtataca cgttgggtaa gacagccgcg
     1801 agctccgcac cccgctgcga gtcctcaacg tccgtcccga tgttgaccac ggccccgacc
     1861 cctgcctccg cagcctccct caccgcctcc tccggcgaaa cgtccagccg caaaaggtgg
     1921 gtatgcgagt ctacgagcat aaggatctgc ctctcccccg agagccacca gcgggagcat
     1981 tatacagagc accgataaca gcggctcatt ctcgcgcccc acgatagtct gggggctcgt
     2041 ctccgggccg cgcgggtcgc atcgccatca cgtggcgcca cgggaagaac acggaccctc
     2101 cacccgggtc caccccttcc ccgctcagca cgacgcccca gtcgttgatc ccttcgagcc
     2161 gcacgatgac ctgatacacg cgtccaccca gcacctccac cagcacctgc tggccgatcc
     2221 actcctcggg cgacttccct gacgcaggca aaatgctccc ttctctacgg ccaggcttcc
     2281 acaagccggg cgtagatctc ccccttcttc cggcccgact cccgcgcggc ccgcgccgcc
     2341 gcgcgcgacg gactctctcc ttcctcaacg tacccccgcg ccagttccac agcctgctcg
     2401 acgctcccgg gaccggcggc ggtcccgcca cgcacgacga gcacgatctc tcctttcgtc
     2461 ccctcggaga aacgccgcgc cgcctccgcg gccgtccccc tgaagacctc ctcgtgcagc
     2521 ttcgtgagct cgcgacacac agcgactaac gtctcgggcg gaagatctcc gagcgtcttc
     2581 cccagcctgt gcggagactc gaagaggatg aaagttgacc tctctttaga gatacgcgcg
     2641 agcagctcgg tgcgttccct gcccttgcga ggcaggaaac cgaggaacac gacgacgtcc
     2701 gcgggcagcc cggacgcaac gagggacgtg atcggggctg atggacccgg cagcacctcg
     2761 acatcgacgc ccttttcgat gcacgccgtg accagacggt agccggggtc cgagacgagc
     2821 ggcatgcccg cgtcggaggc gagcgcgatc tcctgcgtgc gcgccctctc ggcgagctcg
     2881 ggggtcaggc gctcctcatt gtgctcgtgg taggagacca gatcgttctt gatgccgtgg
     2941 tgggcgagca ggcggccggt gcgccgggtg tcctcgcagg cgacgaggtc cgcctcgctc
     3001 aagacgcgca gagccctgag ggtgatgtct tcgaggttgc cgatcggggt cggcacgact
     3061 acgagtacca cgctctagct ctccagcacg tactccccgg aggaagggtc gcgggcgagc
     3121 gccgcggccc cgagcacacc agacatcggg ccgagcgtcg cttccttgat ctggacgagg
     3181 tcgcgagaag gcgggcgtgc gcgcagacgc acctcctcgc gggcggcctc gaggattagc
     3241 tctccggctg ccatgacgcc gccgccgacg gtgatcacct ccgggttgaa gacgttgacg
     3301 aagccggcga gcccgatgcc gagccaccgc cctgcttcct caagaacggc gacggcgacc
     3361 ccgtcaccct cgcgggcgag gcgcgtcacg tcctcgccga gcacttcccg gtcggcggcg
     3421 aggcggccga gggcggagcc gggatgttcg ctggccagct cgcgggcgcg
//
LOCUS       DASTUV010000410         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1690475, whole genome shotgun sequence.
ACCESSION   DASTUV010000410 DASTUV010000000
VERSION     DASTUV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690475"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFT03_08270"
     CDS             <1..606
                     /locus_tag="VFT03_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848180.1"
                     /translation="EGMRGRLDRVADIEVVGEAASGDEAVEMAHKLEPDVILMDIKMP
                     GLNGIEATREILRANPRVGVLMLTMFEDDDSVCAAMRAGAKGYLLKDSGGEGVMHAIR
                     AVASGEAVFGPGVAERIMGFFSVPRPRQQRLFPELTEREEEILSLVAQGKSNQEIARQ
                     LFVSLKTVRNHVSNILMKLQVADRAQAVIRARESGLGHDST"
     gene            complement(678..1865)
                     /locus_tag="VFT03_08275"
     CDS             complement(678..1865)
                     /locus_tag="VFT03_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU4848181.1"
                     /translation="MNSQSDVEERSSHPAYGSEHFDTVIIGGGQAGLSVGYHLKEQGR
                     PFVILDANERIGDSWRKRWDSLRLFTPARYDGLAGWSFPAPVLSFPTKDEMADYLESY
                     AARFDLPVRTGVKVDGLSRQDDRYILSSGNLCFEAEHVVIATGSNQVPKVPGFADDFH
                     PSIVQLHSSQYRNPSQLQEGAVLVVGAGNSGAEIAFEVSRARPTFLSGKPSGEIPVRH
                     GPAMARFVFPVIRFVGHHVLTLRTPIGRKVQPRFISHSAPLIRVKLKDLAAAGVEQVP
                     RTVGVESGRPALEDGGVLEVSNVIWCTGFREEFPWVDLPIFGEGGRPLHERGVVVGEP
                     GLYFVGLLFQYAVSSDVLPGVGRDAEYIAKHIASREPHGRPTAHDALAGAQRPEVPDR
                     ARA"
     gene            complement(1920..2348)
                     /locus_tag="VFT03_08280"
     CDS             complement(1920..2348)
                     /locus_tag="VFT03_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEU4848182.1"
                     /translation="MALLEEAGRAEDWYVAYRHDDPDRSCSLQVFVWPPGSATSIHDH
                     SSWGAFCCVVGSILEERYERADDGSLPEYARLKKLWRLEWGREDGISTVQPYEGGIHR
                     VGNPTQEPAISVHLYGPRLGEIDGRDYDPSRDFVCDRTED"
BASE COUNT          524 a          821 c          843 g          483 t
ORIGIN      
        1 gagggcatga ggggtcgcct ggatcgggtg gccgacatag aggtggtggg agaagcggcg
       61 agcggcgacg aagcggtgga gatggctcac aaactcgagc ccgacgtcat cctcatggac
      121 ataaagatgc ccggcctgaa cggcatagag gccacccgcg agatcctgcg ggcaaatccg
      181 cgtgtcggcg tgttgatgct aaccatgttc gaggacgacg attcggtctg tgccgctatg
      241 cgcgccgggg ccaaaggcta cctcctgaaa gattccggag gcgagggggt gatgcacgcc
      301 atccgggccg tagcaagcgg cgaggctgtc ttcggccccg gagtagccga acgcatcatg
      361 ggcttcttct ccgtcccaag gcccaggcag caacgcctct tcccggagct aaccgagcgc
      421 gaagaggaga tcctctctct cgtggcgcag ggcaagagta accaggagat agccaggcag
      481 ttattcgtga gtctcaagac ggtgagaaac cacgtctcga acatcctcat gaagctgcag
      541 gtagcggata gggcgcaagc agtgatccgc gctagagaat ccggtctggg gcacgatagc
      601 acgtagccga caaggtccgc gttccagact ttctcgtccg tctgggttga gcctcgaagg
      661 ctttgagcgt cggcgcctca ggccctcgcg cgatcaggta cctcaggacg ctgtgctccg
      721 gccaaggcgt cgtgcgccgt gggtcggccg tgcggttcgc gcgacgcgat atgcttcgcg
      781 atgtactccg catccctccc tacgccgggg agaacgtcag acgacactgc gtactggaag
      841 agcagcccta cgaagtacag gccaggctct ccaaccacga cgccgcgttc gtgcaaggga
      901 cgtccaccct caccgaagat agggagatcg acccaaggga actcctcccg gaaccccgtg
      961 caccagatca cgttcgacac ctcaaggact cctccgtcct cgagcgctgg acggccgctc
     1021 tctacgccga cggtcctcgg cacctgctcg actccagcgg cggcgaggtc tttcaacttg
     1081 acgcggatga gcggcgcgct gtgggagatg aatctaggtt gaaccttccg cccgatgggc
     1141 gtcctcaacg tcagcacgtg gtggccgacg aaccgtatca cggggaaaac gaatcgcgcc
     1201 atagcgggtc cgtgacggac ggggatctct cctgagggct tgccagagag gaatgtggga
     1261 cgcgcgcgtg acacctcgaa agcgatctcc gctccagagt tgcccgcgcc aaccaccaga
     1321 acggcacctt cctgaagctg agaggggttg cggtactggc tcgaatggag ctgcacgatg
     1381 ctcggatgga agtcgtcggc gaagcctgga accttcggga cttggttgga gcccgtcgcg
     1441 atgaccacgt gttccgcttc gaaacagagg ttgccggacg acaagatgta gcggtcatct
     1501 tgcctggaga gtccgtccac cttgacgccg gttcgaactg gcagatcgaa tcgggcagcg
     1561 taggactcga ggtagtcggc catctcgtcc ttcgtgggaa acgacaagac aggtgctggg
     1621 aagctccagc ccgccaaccc gtcgtatcgg gcaggggtga acaggcgaag cgagtcccag
     1681 cgcttgcgcc aggagtcacc gattcgctcg ttggcatcca ggattacgaa cggacggccc
     1741 tgctccttta agtggtagcc gaccgagagg ccagcttggc caccgccgat gatcacggta
     1801 tcgaagtgct ctgagccgta ggcaggatgt gagctgcgct cctcaacgtc gctctgactg
     1861 ttcatctgct cctccttgct ggcttctcct cgcctccggg ctgctcacct gccactgacc
     1921 taatcctccg tccggtcgca gacgaagtct cgggaggggt cgtagtcgcg tccgtctatc
     1981 tcaccaagcc gcggaccgta aaggtgcacc gagatggctg gctcctgggt cgggttgccc
     2041 acgcggtgaa tgcccccctc atacggctgc acggtcgaga tcccatcctc ccttccccac
     2101 tcgagccgcc agagcttctt caagcgggca tattctggca gggagccgtc gtctgcacgc
     2161 tcgtagcgct cttccaggat ggatcccacg acacaacaga acgctcccca ggaggaatga
     2221 tcgtggatac tggtcgcgga gccagggggc cagacgaaga cctgcagcga gcaggagcgg
     2281 tcggggtcat cgtggcgata ggccacgtac cagtcttcgg ctcttcccgc ttcctccaag
     2341 agtgccaaga catgcggctg taggaactcg ggatccctca ccaagcgcga cagcataggt
     2401 atggcgtccc gcaaggtcgg atccggagga aggacattac gggctatcgg gtcaagcgtg
     2461 aacgtcttct ctggctcagt atcaaggccg tgtcccaaaa ctatttcgct cattggcgtc
     2521 cggcctcctc gcgatcgagg agcgtcgaga acttgtcggt aaatggaggc tgctcttccg
     2581 cgtcctcccc agggtgaaag ccaatccggt acgccaggcc gtagttcatc ggcgcctcct
     2641 tgtcgtcggt acggggttgc acaaccgaca a
//
LOCUS       DASTUV010000411         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1480520, whole genome shotgun sequence.
ACCESSION   DASTUV010000411 DASTUV010000000
VERSION     DASTUV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480520"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..605)
                     /locus_tag="VFT03_08285"
     CDS             complement(213..605)
                     /locus_tag="VFT03_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU4848183.1"
                     /translation="MATNTELDENFIAKQKERLQSLRDELLRIMRVAEEDERTRTEQD
                     ADFTEHDAGDMSRDIFDREVDATIVEQVEQRLAIVERAMQKIEEGTYGLSDVSGEPIP
                     RGRLEAVPEAIRTVEEQQRFERERHPPF"
     gene            complement(675..2030)
                     /locus_tag="VFT03_08290"
     CDS             complement(675..2030)
                     /locus_tag="VFT03_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4848184.1"
                     /translation="MLELQADTLVVFVSDSHIGGDPGCDGFESPAELEALFEELRARE
                     GPVELILAGDFFDFLQIGKVPNGENRASLTMDRPEYRDLFAALGRFREAEGKRVIYLP
                     GNHDAESFWNPGIQKTLRERGLVDEFAYYYLASVETGGERRVIYCEHGNQFDPANTVE
                     DYHDSLDTPLGHHVVMDFTRRVAPLGVISGGLDLSEIKMVYPLVAIPRWIASRYFYDF
                     VGKVITYLLFPLLLTYAVYRLATYSLAVSRGDAATVFGAFRELPQIHQLFVDFVGFGL
                     IILLIFAIFFLIIRSAVRRTLATVSPGGRLRYSPAEASQNAIRSILTGGARPPMDPEI
                     DPTTIDVFVSGHTHQPSLVEAERPEGRHAVMVNSGCFLRQLQPVLPRLKGPPVFVSKF
                     VLNHVRVFAEDGQLRAELWEQPKIARQRLSRIERLLSWRRRPPQPHPKAKPRVMTSAT
                     V"
     gene            complement(2155..3273)
                     /locus_tag="VFT03_08295"
     CDS             complement(2155..3273)
                     /locus_tag="VFT03_08295"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU4848185.1"
                     /translation="MTQRVSGLPTTTDLVSDLDHGFSLPASWYTDPAIVDLERERIFR
                     HSWQYVGRTAQVSNVGDYFVAMACDLPVVVVRSEEGLRAFVNVCRHRRHEVMSGAGNK
                     RVLQCPYHAWTYGLDGCLRAAPRSEREEGFDKEDFPLVPVQVDTWGPWVFVNPDLEAV
                     PLASILGELPQIIAQSGLDPNELQFWQRDEWVRNANWKAMLENYLECYHCPTQHPGFS
                     AIIDVNPDHYSLEPHEWFLSQVGPVRSAVLEGKSKKKLAYDARGAVTQSQYHFLWPNF
                     TISINPGYPNLSVAVWMPDGPGRSRGFSEQYFGVGVPEDFARDMMAFDDQVGAEDDAL
                     TDSVQRGLRAGVPAQGRFLVESEQLVIRFQKMVLSALS"
     gene            complement(3386..3922)
                     /locus_tag="VFT03_08300"
     CDS             complement(3386..3922)
                     /locus_tag="VFT03_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848186.1"
                     /translation="MADRRVLRVTLRYTDINSLFNKPDVSPFSEEYNDYSTTSGIEHI
                     YGELQANPSLKRVEATILLPREQITSTLEQRTREAIRRYCLARSREVGQSERALRWRA
                     LRALAFGLILFAVNVLLQDRMQDILVLKLIGEGLDILIWVALWFPLDALFFGLLSYDL
                     NSESYKRASEMQLKIEPT"
     gene            complement(4033..4149)
                     /gene="rrf"
                     /locus_tag="VFT03_08305"
     rRNA            complement(4033..4149)
                     /gene="rrf"
                     /locus_tag="VFT03_08305"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4229..>4319)
                     /locus_tag="VFT03_08310"
     rRNA            complement(4229..>4319)
                     /locus_tag="VFT03_08310"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          817 a         1361 c         1300 g          841 t
ORIGIN      
        1 gagaaacacg cctaccgcgg cgagcggcgc gatcaggcgc ctgaacgcgc tccagagccc
       61 gccctcggat ctcgggtatt cgggctcagg cctgcgctct tcgaggtcgc tgcggaagac
      121 gggctcacgt gcgccatctt catcgaacgg gtcgctaaaa cggggacggt ccatgaccag
      181 attatatccg ccacacgacg gaccggcggg cgctagaacg gcgggtggcg ctcccgctcg
      241 aagcgctgct gctcttcgac ggttcggatc gcctccggga cggcctccag ccggccgcgg
      301 ggtatgggct ctccactcac gtccgagagc ccgtaggtcc cctcctctat cttttgcatc
      361 gccctctcga cgatcgcgag ccgctgctcg acctgctcga cgatggtagc gtcgacctcg
      421 cggtcgaaaa tatcccggct catgtccccg gcgtcgtgct cggtgaaatc cgcatcctgc
      481 tcggtccggg tgcgctcgtc ttcttctgcg acgcgcatga tccggagtag ctcgtccctc
      541 aggctctgca gccgctcctt ctgtttcgcg atgaagttct cgtcaagctc ggtgttggta
      601 gccataactc ttccttgatc cctccagagt aggattttgc aaggtcgaga aggtattccc
      661 gctacggtcc aatactacac ggtggcggac gtcatcacgc gcggcttcgc cttggggtgc
      721 ggctgaggcg gcctgcgcct ccatgatagg agacgttcga tccggctcag gcgctgccgc
      781 gcgatcttgg gttgctccca cagctcggct cggagttgcc cgtcttcggc gaagacgcga
      841 acgtggttca gtacgaactt cgagacgaag accggaggac ccttcaggcg cggcaagacg
      901 ggctggagct ggcgcaggaa gcaccccgag ttgaccatca ccgcatgacg gccctccggc
      961 ctctccgctt cgacgagcga aggctggtga gtgtgccccg agacgaagac atcgatggtc
     1021 gttggatcta tctccgggtc catcggcggc ctggcgcctc cggtcaggat agaacgtatc
     1081 gcgttttgcg acgcttcagc gggcgagtag cggagcctgc cgccagggct cacagtcgcc
     1141 agggtcctgc ggaccgcgct ccggattatg aggaaaaaga tcgcgaagat tagcagtatg
     1201 atcagcccga agcccacgaa atcgacaaac agctggtgta tctgcggcaa ctcccggaag
     1261 gccccgaaga ccgtcgccgc atcgccgcgg ctaacagcca gagaataggt ggccaggcgg
     1321 tagacggcgt acgtaaggag cagcggaaag agcaggtagg tgatcacctt cccgacgaag
     1381 tcgtagaagt agcggctggc gatccagcgc ggtatcgcga cgagcgggta gaccatcttt
     1441 atctcggaga gatctaggcc ccccgagatc acgccgagcg gtgcgacccg acgcgtgaag
     1501 tccatgacga cgtggtggcc gagcggcgtg tcaagggaat cgtggtagtc ctcgacggtg
     1561 ttcgccgggt cgaactggtt cccgtgctcg cagtagatga cgcgcctttc ccctcccgtc
     1621 tccaccgagg ccaggtagta gtaggcgaac tcgtccacga gcccccgctc ccgcaatgtc
     1681 ttctggattc cggggttcca gaacgactcc gcgtcgtggt ttccggggag gtagatcaca
     1741 cgcttcccct ccgcctcgcg gaagcgcccg agcgccgcga agaggtctct gtactccggg
     1801 cggtccatcg tcagcgacgc cctgttctcc ccattaggca ccttaccgat ctgcaggaag
     1861 tcgaagaagt cgccagccag gatcaactcg accgggccct cgcgggccct cagctcctcg
     1921 aagagcgcct cgagctctgc cggcgactcg aaaccgtcgc agccggggtc ccccccgata
     1981 tgggagtcgg agacgaagac gaccagcgtg tccgcctgta gttcgagcat cggcgcatta
     2041 tatccggccc gcagacctct cagtataaag ctggccgttt cgccgagaat ccgatgtggt
     2101 tcccaagcta ctacctaacc tctacgaccg gctttctccg gcggtaaccg agcttcagga
     2161 aagggccgag agtaccatct tctggaagcg gatcaccagc tgttcgctct cgaccaggaa
     2221 gcggccctgc gcgggcaccc ccgcacgcaa gccgcgctgg acggagtcgg tcaaagcatc
     2281 gtcctcggct cccacctggt cgtcgaacgc catcatgtct ctggcgaagt cctccggcac
     2341 acccacgccg aagtattgtt ccgagaaccc gcggcttcgc ccgggtccgt ccggcatcca
     2401 gacagcgacc gacagattgg ggtagcccgg gttgatgctg atggtgaagt ttggccagag
     2461 gaagtggtat tgggactggg taaccgcccc gcgtgcgtcg taggcgagct ttttcttgct
     2521 cttgccctcc aaaacggccg accttaccgg cccgacttgg ctgaggaacc attcatgagg
     2581 ctctagggaa tagtggtccg ggtttacgtc tatgatggcg ctaaaaccgg gatgctgcgt
     2641 cgggcaatga tagcactcga ggtaattctc gagcatcgcc ttccagttgg cattgcgcac
     2701 ccattcgtcc cgctgccaga actgaagctc gttgggatcc aggccgctct gagcgatgat
     2761 ctgcggcaac tcacccagga tgcttgccag gggtacggcc tcgaggtccg ggttcacaaa
     2821 aacccatggc ccccaggtat ccacctggac aggtacgagc ggaaaatcct ccttgtcgaa
     2881 cccctcttcg cgctcagagc gaggtgctgc cctcaggcac ccatcgaggc cataggtcca
     2941 ggcgtggtat gggcactgca ggactcgctt gttacccgca ccgctcatca cctcgtgcct
     3001 gcggtgccgg cagacgttga cgaaggccct caacccctcc tcgcttcgca cgacgaccac
     3061 cggcaagtcg cacgccatgg cgacaaaata gtcgcccacg ttcgaaacct gtgcggtccg
     3121 gcccacatat tgccagctgt ggcggaaaat tcgctcgcgt tccaggtcca ctatcgccgg
     3181 gtcggtgtac caggaagccg ggagggagaa cccgtggtcg aggtcgctga ccaggtcggt
     3241 ggtcgttggc agtcccgata ctcgttgcgt catggccgat ctccttgtgc tgagggaggc
     3301 atggagggat gtgtcataac tcctctctag ctgcgccagg gggccttagc cagggggcct
     3361 tagccagggg gccttagcca ggggcttagg tgggctcgat cttcagctgc atctccgaag
     3421 cgcgcttgta gctctccgag ttcagatcgt aggacaacag cccgaaaaag agggcgtcta
     3481 gcgggaacca gagggcgacc cagataagta tgtccaatcc ttcaccgata agcttgagca
     3541 ccagtatgtc ctgcattcta tcttgcagga gtacgttgac ggcgaagagt atgagcccga
     3601 acgcaagagc gcgcaacgcg cgccagcgca acgcgcgctc gctctgtccc acttcccgac
     3661 tgcgggcgag gcaatatcgt cggatggctt ctcgggtccg ctgctccagg gtcgacgtga
     3721 tctgctcgcg cgggagcagg atggtcgcct cgactctctt gagcgacggg ttcgcctgca
     3781 attcaccgta gatgtgctcg atgcccgatg tcgtgctgta gtcgttatat tcctcggaga
     3841 acggagagac gtcgggtttg ttaaagagcg agttgatatc cgtgtaccgt agcgtcactc
     3901 ggagcaccct gcgatccgcc acctatgcac ctcccggccc tccagctgca ccggcctcca
     3961 aatcgcacag tctagcttcg ccagattgct ttgtcaccaa cgataattgg gggatcaccc
     4021 tacacaataa aaaatgggcg acgacctact ctcccacagg gtctcccctg cagtaccatc
     4081 ggcgctggcg ggcttaactt ctctgttcgg aatgggaaga ggtgtatccc cgccgccatc
     4141 gtcaccctat ctaaaatagg cgtgtattcg gttgtaagac ccaaagagcg gttgggcctg
     4201 atctttggaa actgcatagc gctactaaac catcaagccc tcgacttatt agtaccactc
     4261 ggctgaacac gtcgccgtgc ttacacctgt ggcctatcaa cctggtggtc taccaggag
//
LOCUS       DASTUV010000412         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1440089, whole genome shotgun sequence.
ACCESSION   DASTUV010000412 DASTUV010000000
VERSION     DASTUV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440089"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VFT03_08315"
     CDS             <1..171
                     /locus_tag="VFT03_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4848187.1"
                     /translation="INKQGTTILLVEQNANIALEIATRGYVLETGTIVNSAPAAELRE
                     DPTIREAYLGEI"
     gene            453..2960
                     /locus_tag="VFT03_08320"
     CDS             453..2960
                     /locus_tag="VFT03_08320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU4848188.1"
                     /translation="MRVYLIDGYSLLYRAFYALPQSIATTSGLPTNALYGFTSMVLKL
                     LDSEEEVGLGVVWDAGIPAFRLEVFPEYKAHRSAMPDELRVQLDHLDEILEAMNIPAI
                     RAEGFEADDALATLSKQVPDGIELFIVTGDQDAMQLVDGRVRVLRTTRGVSETKTYGR
                     QEVIEEYGVTPEQIPDYKALVGDPSDNIPGVRGIGPKGAANLLQEFDSVQGIYDHLDA
                     VAAKGTRKKLEEGRESAFLSLELARMRFDAPVTFDGEALRFEGVSPEILDVLLRRYEF
                     RSLEPRLAELPVVGGGDLPTPERLAVRFSEEPIEPSFEPVAVAPVGDGRWAVAVADDE
                     VRISEGLPDRPLRVHDAKKAGVRGAHFDTFLAAYLIKPGLGSYELDALASERGLAEVE
                     VGVDDPAVREAARRAATIQNLSPRLVEELEEMGLARLFYDIELPLADVLGEMEKFGMP
                     VEARVLEEVGEEIEGRVEELERRIYEDAGVEFNIGSPKQLGEVLFERLSIPPLRKTKT
                     GYSTDARVLQQLAIEHPIAERIIEYRELTKLRGTYIEGLSKLIGEDGRIHTTLNQTAA
                     ATGRISSESPNLQNIPVRTPLGVRIRDAFTASPGRRLVVADYSQIELRILAHMTGEPA
                     LVESFTKGEDIHTRTAAEVFDTRIEMVTPELRRRAKMVNFGILYGISGFGLASRLGNV
                     HPAEAERYIKRYFERYPKVTEFMKETLQEAEELGYATTLFGRRRYVPELGSSNKNIRR
                     LGERLAFNARVQGSAADIIKVAMVDLAPRLRPLGADMIMQVHDELVFDVDEDRVDVVA
                     GLAADRMVAAYDLEPPLEVEVKTGERWGRVEPLEKRV"
     gene            3055..>3950
                     /locus_tag="VFT03_08325"
     CDS             3055..>3950
                     /locus_tag="VFT03_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU4848189.1"
                     /translation="MTDTTQNVSRELTMKDFFREENGEIVPIDESVLIDFKDGDIVEG
                     DVVRIDKDEVLVDIGYKSEGLIPSNELTIRKGADPHEVVELGQHIEALVLQKEDADGR
                     LILSAKRAAFERAWNRIEEAYNDQRTVEGPVIEVVKGGLILDIGLRGFLPASLVDIRR
                     VRNLEAFLGQKLECKVIELNRSRNNVVLSRRAVLEEERKEERERILTSLEEGMIVRGT
                     VSNLVDFGAFVDLDGIDGLIHISELSWNHVDHPSEVVQVGEEVEVKVLEVDRDRERIS
                     LGLKQTRKDPWQEIVERVNVGE"
BASE COUNT          779 a         1218 c         1319 g          634 t
ORIGIN      
        1 atcaacaagc agggcaccac gatcctgctc gtcgagcaga acgccaacat cgccctcgag
       61 atcgcaaccc gcggctacgt cctcgagacg ggaaccatcg tcaactccgc tccagccgcc
      121 gagctgcgcg aggacccgac gatccgcgaa gcttacctcg gcgagatcta gtttcttcac
      181 acatcgggtt cgaccatccc gcgggcctat tggaggcttg ccggcaaaac tcccctatca
      241 accccgaata tgcaaccctt cccgtagagg agggcttcaa ctggttctcc aaccttggta
      301 gggcctgtct cgaccagcta tacctcgtgg tcttcgggcg gctcgaccgt gtgcctggcc
      361 gtgagaacac accggcctga tggccgcgcg gcgccggatt tactgcccgt tcgccgtttt
      421 cgtgggctcg cttcgcggta taattcataa gaatgcgcgt ctacctcatc gatggctact
      481 ccctgctcta ccgggcgttc tatgcgctgc cgcagagcat agcgacgacc agcgggctgc
      541 ccacgaatgc cctctacggc ttcacgagca tggtcctcaa gctgctcgat tcggaggaag
      601 aggtgggtct cggggtggta tgggacgccg gcataccggc gttccggctg gaggtcttcc
      661 ccgagtacaa ggcccaccgc tccgccatgc ccgatgagct cagggtccag ctggaccacc
      721 tcgacgagat cctcgaggcg atgaacatcc cggccatcag ggccgagggg ttcgaggccg
      781 acgacgccct ggcgaccctg agcaagcagg tgccggatgg tatcgagctc tttatcgtga
      841 ccggagacca ggacgcgatg cagctcgtgg acggacgcgt ccgggtgctc aggaccaccc
      901 gcggcgtctc ggagacaaag acctacggac ggcaggaagt aatcgaggag tacggcgtca
      961 cgccggagca gatcccagac tacaaggcgc tcgtgggcga cccctcggac aacatacccg
     1021 gcgtcagggg catcggcccc aaaggcgccg cgaatctgtt gcaggagttc gattcggtgc
     1081 aggggatcta cgatcatctg gacgccgtcg ccgccaaggg cacgcgcaaa aagctggagg
     1141 aagggcggga gagcgccttc ctctcgctgg agctcgcccg catgcgcttc gacgcccctg
     1201 tcacgttcga cggggaggcg ctcagattcg agggcgtgtc acccgagatc cttgacgtgc
     1261 tcctcaggcg ctacgagttc aggagcctcg agccccgact ggccgagcta ccggtagtcg
     1321 gcggcgggga cttgccgacg ccggagcggc tggcggtacg cttctccgag gagcccatcg
     1381 agccgagctt cgagcccgta gccgtggcgc cggtcgggga cggccggtgg gcggtcgcgg
     1441 tggcggatga cgaggtcagg atctcggaag ggttgccgga ccgacccctg agggttcacg
     1501 acgcgaagaa ggccggggtc aggggcgcac acttcgacac gttcctggcg gcgtacctga
     1561 tcaaacccgg cctgggtagc tatgagctgg atgccctcgc ctcggagcgt ggcctggccg
     1621 aagtcgaggt cggggttgac gaccccgccg tcagggaagc ggcgcggcgg gccgcgacga
     1681 tccagaatct ctcaccgagg ctcgtcgagg agctggagga gatgggtctc gcgaggctct
     1741 tctacgacat cgagctaccg ctcgccgatg tgcttgggga gatggagaag ttcgggatgc
     1801 cggtcgaggc gagggtcctg gaagaggttg gcgaggagat agagggccgg gtcgaggagc
     1861 tggaaaggcg catctacgag gacgccggcg tcgagttcaa cataggctcg cccaagcagc
     1921 ttggcgaggt gcttttcgag aggttgagca tcccgccgct caggaagacg aaaaccggct
     1981 actcgacgga cgccagggtg ttgcagcagc tcgccatcga gcaccccatt gccgagcgca
     2041 tcatcgagta tcgagagctg accaagttgc ggggcacgta tatagaaggg ctatcgaagc
     2101 taataggcga ggatgggcga atccacacca ccctcaacca gacggcggct gctacggggc
     2161 gcatctccag cgaatccccg aacctccaga atatcccggt acgcaccccg ctcggcgtgc
     2221 gtatcaggga cgcgttcaca gcctcgcccg ggcgcaggct cgtggtcgcc gactactcgc
     2281 agatagagct caggattctg gcgcacatga ccggggagcc cgcgctcgtc gagtcgttca
     2341 cgaagggcga ggacatccac actcgcacag ccgccgaggt gttcgacacg cgcatagaga
     2401 tggttacgcc cgagctcagg cgacgggcga agatggtcaa cttcggcatc ctgtatggca
     2461 tctcgggttt cgggctcgcg agccggctcg gaaacgtcca cccggcggag gccgagcgct
     2521 acatcaagcg ctacttcgag cgctacccga aggtgaccga gttcatgaag gagaccctgc
     2581 aggaggccga ggagctcggc tacgcgacta cgctcttcgg gcggcggcgc tacgtgcccg
     2641 agctcgggag ctccaataag aacatccgga ggctagggga gaggctcgcg ttcaacgctc
     2701 gcgtgcaggg gtcggcggcg gacatcatca aggtggcgat ggtcgacctc gcgcccaggc
     2761 tgcgcccgct tggtgcggac atgatcatgc aggtgcacga tgagctggtc ttcgacgtag
     2821 acgaggacag ggtcgatgtg gtggccggtc ttgccgccga caggatggtc gcggcctacg
     2881 accttgagcc gcccctggag gtcgaggtca agaccggcga gcgctggggc cgggtcgagc
     2941 cgttggagaa gcgggtgtga tcacgacctg acgaaaaccc ggtttcggac gccacctgcg
     3001 caaaactgta taatctgcac cgacccgcaa tggaaaaagg aaagtaacca ctacatgact
     3061 gacaccactc aaaacgtatc ccgggaactg accatgaagg acttcttccg ggaagagaat
     3121 ggggagatag tccccataga cgagagcgtc ctcatcgact tcaaggacgg cgacatagtc
     3181 gagggcgacg tcgtccgcat agacaaggac gaggtcctcg tggacatcgg ctacaagtcc
     3241 gaggggctca tcccctctaa cgagctcacc atccgcaagg gcgccgaccc gcacgaggtc
     3301 gtcgagctcg ggcagcacat cgaggccctc gtcctgcaga aggaggacgc cgacgggcgt
     3361 ctgatcctct ccgccaagcg tgctgccttc gagagggcct ggaaccgcat cgaggaggct
     3421 tacaacgacc agcgcaccgt cgagggcccc gtcatcgagg tcgtcaaggg cggcctgatc
     3481 ctggacatcg gcctgcgcgg cttcctcccc gcgagcctcg tggacatccg gcgcgtcagg
     3541 aacctggaag ctttcctcgg ccagaagctc gagtgcaagg tcatcgagct gaaccggagc
     3601 cgcaacaacg tagtgctttc caggcgcgcg gtcctcgagg aggagcgcaa ggaggagcgc
     3661 gagcgcatcc ttacctcgct agaggagggc atgatcgtac gcggcaccgt ctctaacctg
     3721 gtggacttcg gggctttcgt ggacctcgac gggatagacg gcctgatcca catctccgag
     3781 ctgtcctgga accacgtcga ccacccgagc gaggtagtgc aggtaggcga ggaggtcgag
     3841 gtcaaggtcc tcgaggtgga cagggaccgc gagaggatct cgctcggcct caagcagacg
     3901 cgcaaggacc cgtggcagga gatcgtcgag cgggtcaacg tcggcgagca
//
LOCUS       DASTUV010000413         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_619338, whole genome shotgun sequence.
ACCESSION   DASTUV010000413 DASTUV010000000
VERSION     DASTUV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619338"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFT03_08330"
     CDS             complement(<1..710)
                     /locus_tag="VFT03_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fuconate dehydratase"
                     /protein_id="HEU4848190.1"
                     /translation="MSTTITGAVALDVRFPTSSDLSGSDAMNEAPDYSAAYVILETDT
                     ELEGHGLTFTIGRGNELCVAAIEALAPLVVGETLESFAEDMGAFWRRVTGDSQLRWVG
                     PEKGVIHLATAAIVNAVWDLYAKSEGKPLWKLLVDMSPEEIVRCVDFRYITDAITPEE
                     ALEILKRNEPTKTAREQEMLVHGFPAYTTSAGWLGYPDEKIRRLSREAVAEGWGHVKI
                     KVGRDLEDDARRAGIIRE"
     gene            complement(715..993)
                     /locus_tag="VFT03_08335"
     CDS             complement(715..993)
                     /locus_tag="VFT03_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848191.1"
                     /translation="MSVPFKRTVETGYDRIAERYLASKNPEDPATLEQLAGELAPGAP
                     MRWSHYEGETSLALLRGAGLEIERAETRTTSGPGGDESWLWVLARKGG"
     gene            complement(990..1745)
                     /locus_tag="VFT03_08340"
     CDS             complement(990..1745)
                     /locus_tag="VFT03_08340"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU4848192.1"
                     /translation="MDRLEGKRAVVTGAGAGIGRSIALRLASEGARVALADVDEEAAE
                     KVASEIDGETLVRRTDVTKAEEVEALVASAVEAWGGLEVMVNNAGVGVAATVVDTTEE
                     DYERVMDVCVRGTLLGMKYAIPAIRDAGGGSVINMSSVAALVGITDRAVYSAAKGAIL
                     SMTRAAAIDHVGEGVRVNCIAPGTVDTPWIDRITSGYDDPEEARAKMQARQPHSRFVT
                     PEEIAAMAAYLASDEAASCIGACMIVDGGVTAR"
     gene            1831..2163
                     /locus_tag="VFT03_08345"
     CDS             1831..2163
                     /locus_tag="VFT03_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4848193.1"
                     /translation="MEPFPDWMKDSVPEVDTPFTGLEGRLLASPHGQAVFFSAAEEFE
                     VPPHAHGAQWGIVVTGRLHLTVDGEPGIYEPGETYDIPSGAEHSARFEAGTSVIDVFQ
                     DPDRYAPK"
     gene            2192..3172
                     /locus_tag="VFT03_08350"
     CDS             2192..3172
                     /locus_tag="VFT03_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEU4848194.1"
                     /translation="MTQRVYVDGPIHEEALGMLRSTTEVVPEAVELGAALPDVHAVLL
                     RTASLDAEAIERARSLRVIARHGVGVDNVAVDVAARRGIPVLITPQANLRSVAEHVFA
                     LALAVSRNLILADDTVRSGRFASRDRLLGRELFGATLGVIGLGRIGGEVARMAAWGFG
                     MRVLGYDPLLSPGQIRERDAEPVETLPDLLRACDLVTVHVPLSGQTRGLLGRRELASM
                     RPGSILIQTSRGGVVDEDALVEALRSGHLAGAGIDVYETEPPPQDHPFFSMEQIVLTP
                     HTAALTEQAMRRMAVDAAKGILDVLEGADPYEPPDGARWQAFGPAAEPEE"
     gene            complement(3233..>3536)
                     /locus_tag="VFT03_08355"
     CDS             complement(3233..>3536)
                     /locus_tag="VFT03_08355"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU4848195.1"
                     /translation="DIEEAVWEEISRIPFGQTRTYGQVSEAIGGAASAVEVGKACAAN
                     PVLLVVPCHRVVGADGSLKGYAGGIRLKERLLAFESSLDPDGPRPRRASPPTPALR"
BASE COUNT          514 a         1239 c         1162 g          621 t
ORIGIN      
        1 tcctccctta tgatgccggc cctccgcgcg tcgtcctcga gatctcgccc gacctttatc
       61 ttgacgtggc cccagccctc cgctacggcc tcgcggctca ggcgcctgat cttctcgtcc
      121 gggtacccga gccagccggc gctcgtcgtg tacgcgggga agccgtggac gagcatctcc
      181 tgctcgcgcg ctgtcttcgt gggctcgttg cgcttcagga tctcgagcgc ttcctcgggc
      241 gttatcgcgt ctgtgatgta tcggaagtcc acgcaacgca cgatctcctc cgggctcatg
      301 tccacgagca gcttccagag tggcttgcct tcggatttgg cgtagaggtc ccagaccgcg
      361 ttcactatag cggcggtagc caggtgtatg acacccttct cggggccgac ccaccgcaac
      421 tggctgtcgc ccgtaacccg gcgccagaaa gcccccatgt cctccgcgaa cgactccaga
      481 gtctcgccga ccacgagggg ggcgagggct tctatggcgg cgacgcatag ctcgttgccc
      541 cgcccgatgg tgaaggtgag gccatggcct tcgagttctg tatctgtctc gaggatgacg
      601 tacgccgccg agtagtcggg agcttcgttc atcgcgtccg agccggagag gtcactggag
      661 gtggggaagc ggacgtcgag ggcgaccgcc ccggttattg ttgtggacac gtcgtcatcc
      721 tccttttcgg gcgaggaccc acagccagga ctcgtcgccg ccgggtccgc tggtggttcg
      781 ggtctccgcg cgctcgatct cgagaccggc gccgcgcagc agcgcgaggc tcgtctcccc
      841 ctcgtagtgg ctccaccgca tgggcgcgcc cggagcgagt tcaccggcca gctgctcgag
      901 cgtcgccggg tcttcggggt tcttgctagc caggtaccgt tcggctatgc ggtcgtagcc
      961 ggtctctacc gttctcttga aaggcacgct caacgggcgg tcacgccacc gtccacgatc
     1021 atgcacgccc cgatgcacga ggcggcctcg tcggaggcga ggtaggcggc catcgcggct
     1081 atctcctccg gggtgacgaa cctgctgtgc ggctggcggg cctgcatctt cgcgcgggcc
     1141 tcctcggggt cgtcgtaacc actcgttatg cgatctatcc acggcgtgtc caccgtgcca
     1201 ggcgctatgc agttcaccct cacgccctcc ccgacgtggt ctatagccgc cgcccgcgtc
     1261 atggagagga tagcgccctt agccgcgctg tagacggcgc ggtccgttat tcccacgagg
     1321 gcggccacgg atgacatgtt gatgaccgac cctccacccg cgtcccttat ggcggggatg
     1381 gcgtacttca tcccgaggag ggtccctctc acgcagacgt ccattacccg ctcgtagtct
     1441 tcctccgtgg tgtccaccac ggtggcagcg acgcccactc ccgcgttgtt gaccataacc
     1501 tcgaggccgc cccacgcctc gaccgcgctc gccaccagcg cttcgacctc ctccgcctta
     1561 gtgacatccg ttcgccgaac cagcgtctcg ccgtctatct ccgaggccac cttctccgcc
     1621 gcctcctcgt ccacgtcggc gagggcgacg cgggcccctt cgctggcgag ccgcagggcg
     1681 atggagcgcc cgatcccggc gccggcgccc gtgaccacgg ccctcttgcc ctcgagacgg
     1741 tccatcgcac cccctgctta ctcgaccccg gagacggtca gtataatgac gcctggatcg
     1801 ggggcgcaaa cctggggaga ggagcttttc atggagccgt ttccggactg gatgaaggac
     1861 agcgtgcccg aagtcgacac gccttttacg gggctcgaag gccggctgct cgcgagcccg
     1921 cacggacagg cggtcttctt cagcgcagcg gaggagttcg aggttccgcc gcacgcccac
     1981 ggtgcccagt ggggcatagt ggtcacaggt cgcctgcacc tgaccgttga cggcgagccc
     2041 ggaatctacg agccgggcga gacctacgac attccctcgg gcgcagagca ctcggctcgc
     2101 ttcgaggccg ggaccagcgt catagacgtc ttccaagacc ccgaccggta cgccccgaag
     2161 tagcgtctgc cctccgctgg gtatgacgag cgtgacgcaa agagtgtacg tggacgggcc
     2221 gatccacgag gaggctctgg gcatgctccg gagcaccacc gaggtcgtcc ccgaggcggt
     2281 cgagttggga gctgcgttgc ccgacgtaca cgccgtgctc ctgcggacgg cctcgctcga
     2341 tgcggaggct atcgagcgtg cgcggtcact gcgggtgatc gcgcgccacg gggtgggcgt
     2401 ggacaacgtc gctgtggacg ttgcggcgag gcggggtatc ccggtcttga tcacgcctca
     2461 ggccaacctg cggtccgtag ccgagcacgt cttcgccctg gcgctggccg tgagccggaa
     2521 cctgatcctc gctgacgaca cggtccgcag cgggcgcttc gcctcgcgcg accgcctgct
     2581 gggccgcgag ctgttcgggg cgacgctcgg ggtgatcggc ctcgggcgca tcggcgggga
     2641 ggtggcccgc atggccgcct ggggcttcgg gatgcgggtg ctcggctacg accccttgct
     2701 gtcccccggc cagatccgcg agagggacgc cgagccggtg gagacgctcc cggatctgct
     2761 gcgcgcctgc gacctcgtca ccgtgcacgt cccgctctcc gggcagaccc gtggtctgct
     2821 cggccgccgc gagctggctt cgatgaggcc ggggtcaatc ctgatccaga cttcccgcgg
     2881 cggggtggtc gatgaggatg cgctggtgga agcgctacgc tccggacacc tcgccggggc
     2941 aggcatcgac gtgtacgaga cggaaccgcc gccgcaagat catccgttct tctccatgga
     3001 gcagatcgta ctcacccccc acacagccgc ccttaccgaa caggccatgc gacgtatggc
     3061 ggtggacgcc gcaaagggca tactggacgt gctcgaaggg gcggatccct acgaaccgcc
     3121 ggatggtgcg cgctggcagg ccttcggccc cgcagccgag ccggaggagt gaacacccgc
     3181 gaagagatca cgcgcctctc gatgcggggg gccggtcatt cggagttccc gtctaccgca
     3241 gtgccggcgt gggtggcgag gctcttcgag gtcgcggccc atccgggtcc aggctcgact
     3301 cgaaggccag caggcgctcc ttgaggcgta tgccgccggc gtagcccttg agagagccgt
     3361 cggcgcctac caccctgtgg caagggacca ccagcaggac agggttggcc gcgcacgctt
     3421 tgcccacctc caccgcgctc gccgcgcccc ctatcgcctc ggacacctga ccgtaagtgc
     3481 gcgtctggcc gaacgggatg cgtgagatct cctcccacac ggcttcctcg atgtcc
//
LOCUS       DASTUV010000414         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_897872, whole genome shotgun sequence.
ACCESSION   DASTUV010000414 DASTUV010000000
VERSION     DASTUV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897872"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFT03_08360"
     CDS             <1..299
                     /locus_tag="VFT03_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848196.1"
                     /translation="ERQMRRRARALSARILWRRLVGRPAPRVFVTHSPPFGLGDAEDQ
                     AHVGFESFLGLIDRYEPSLWLHGHVHLYGPEGQRVLRRGETKVVNVFGHRIIEV"
     gene            complement(409..906)
                     /locus_tag="VFT03_08365"
     CDS             complement(409..906)
                     /locus_tag="VFT03_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEU4848197.1"
                     /translation="MNKRQSSSTAPSVPTALMKGPERLKAKDAFFKVGTGIHRAIFKA
                     SKGRIFGKAFGMPVAELVTTGRRSGKKRSTMLAVPISDGDRLVLVASFGGDDRHPAWY
                     LNLKTNPEVKVTIAGSTRSMIARIATEEERAELWPRITSVFEGYARYQKRTERPIPVV
                     ILEPS"
     gene            complement(1035..1727)
                     /locus_tag="VFT03_08370"
     CDS             complement(1035..1727)
                     /locus_tag="VFT03_08370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848198.1"
                     /translation="MSTTTEESRKPYLPAMGHDRLLPLYDPLQRLLGLGSVHRWLVDQ
                     ARIRQDQRVLEIGCGTGNLAILIKRLHPGTQVIGIDPDPKALARAQRKAGREAISVRL
                     DRGFAEELPYPDTSFDRVLSALMFHHLGPKEQVASLGEVRRVLKPGGSLHLLDFGGAK
                     VRSDGFIARLHHRSERLQNNFGDRIPTLMREAGFADPTEVAHRVTIAGRVINYHRASA
                     PRRESDARHSTS"
     gene            1904..>2193
                     /locus_tag="VFT03_08375"
     CDS             1904..>2193
                     /locus_tag="VFT03_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEU4848199.1"
                     /translation="MGATRILGRRDDGPPVYTYEAVPGVPPVSTMRLGQSLSEVRPGA
                     HAHDFLVLAYVERGGGSMWLGAREWQIEAGDVYLIAPGEVVGVGDDAGSLHEA"
BASE COUNT          355 a          663 c          734 g          441 t
ORIGIN      
        1 cggagcgcca gatgcgccgc cgggccaggg cgctctcggc ccgcatcctg tggcgcaggc
       61 tcgtcggacg tccggcaccg cgtgtattcg tcacccactc cccgcccttc ggcctcggcg
      121 acgccgagga ccaggcccac gtaggcttcg aatccttcct cgggcttata gaccgctacg
      181 agccctccct gtggctgcac ggacacgttc acctctacgg gccggagggc cagagggtat
      241 tgcgccgggg tgagaccaag gtcgtcaacg tgttcgggca caggatcatc gaggtgtgaa
      301 acacccgccg gcgtccttgt tgtcggaggc cgaagacgcg acgtgagcga ggggtagcgt
      361 tatatacgcc gcggcagtgg atgccgggag gttccactcg acgcgtggct agctgggttc
      421 gaggatgacc accggtatcg gtctctcggt ccgcttctga tagcgggcgt agccctcgaa
      481 caccgaggtg atccggggcc agagctctgc cctttcctct tcggtggcga tgcgggcgat
      541 catggaacgc gtcgagcccg cgattgtgac ctttacctca gggttggtct tgaggttgag
      601 gtaccacgcc gggtgccggt cgtcgccgcc gaaggaggcc acaagcacta gacggtcgcc
      661 gtcggagatc ggtaccgcga gcatcgtcga gcgcttcttg cccgacctgc gcccggtggt
      721 gacaagctcg gctacgggca tcccgaaggc tttgccgaag atccgtccct tcgaagcttt
      781 gaagattgcc cggtggatac cggtgcccac cttgaagaac gcgtccttgg ccttcaggcg
      841 ctcaggcccc ttcatcaggg ccgtggggac actcggtgcg gtgctggaac tctggcgctt
      901 gttcatcttg acctactttc cttgctgagg ttgatgttgg catcgctgcg ggtgcggcgt
      961 gacgaccggt ctgcggtaac cagaagtcgt cgaggcatct ctcgacgctc tacttctgga
     1021 ggcgcccgcg cgggtcagct cgtcgagtgg cgggcgtcgg actccctacg cggcgcgctc
     1081 gcgcggtggt agtttattac gcgcccggcg atcgtgactc gatgggcgac ctctgtcgga
     1141 tccgcgaaac ccgcctcgcg catgagcgtc ggaatgcggt ctccgaagtt gttttgcagg
     1201 cgctcgcttc tgtggtgcag acgagcgata aatccgtcgg accgcacttt cgctcctcca
     1261 aagtcaagca agtgcaggga gccgccgggt ttgagtaccc gccgtacctc acccagcgac
     1321 gccacttgct ccttcggccc gaggtggtgg aacatcaagg cggatagcac ccggtcgaag
     1381 gatgtgtccg ggtacggaag ttcctccgcg aacccgcgat ccaatcgcac ggagatcgcc
     1441 tcccgtccgg cctttcgctg cgcccgggcg agcgccttcg ggtcgggatc gatcccgatc
     1501 acctgggtgc ccggatgaag acgtttgatg aggatcgcca ggttcccggt gccgcagccg
     1561 atctcgagaa ccctctggtc ctgccgtatg cgggcttgat cgactagcca tcggtggacc
     1621 gaccccaggc cgagcaatct ctgaagcggg tcataaagtg ggagcaaccg atcatgtccc
     1681 attgccggta ggtagggctt gcgggattct tccgtcgtcg tgctcatacc cgtccttcct
     1741 ctcgcgtctt tgcgacgatt ttgagtacgg aaccagtatc cagtctcatg tcggaggcgc
     1801 aaagggcgaa tgtttggact ttttgggacg attttcggtt tatcataggt tgtgggccgc
     1861 tcgcggagcc acggctccat cgttgtggag acaaaggatt gtcttgggtg ccacgaggat
     1921 actgggaagg cgagatgacg ggcctccggt ctacacttac gaggcggtgc ccggagtacc
     1981 gccggtgagt accatgaggc tgggccaaag tttatcggag gtgcggccag gtgctcacgc
     2041 tcacgacttc ctagtgctcg cctacgtcga acggggcggc ggctcgatgt ggttgggggc
     2101 ccgagagtgg caaatagagg cgggcgacgt gtacctcatc gcccccggcg aggtggtggg
     2161 agtgggcgat gatgcgggca gcctccacga ggc
//
LOCUS       DASTUV010000415         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_834578, whole genome shotgun sequence.
ACCESSION   DASTUV010000415 DASTUV010000000
VERSION     DASTUV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834578"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFT03_08380"
     CDS             <1..365
                     /locus_tag="VFT03_08380"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU4848200.1"
                     /translation="VDFMQMTLAKSDLRIAETYTSLVSNSAARERLWNRISAEHAACV
                     EALLKITGHESLLDDSPVLQRSIRLRNPYVDPLSYVQVSLLRRLRALPEDSPKRQTTL
                     NTLLLTISGISSGMLNTG"
     gene            complement(411..1358)
                     /locus_tag="VFT03_08385"
     CDS             complement(411..1358)
                     /locus_tag="VFT03_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEU4848201.1"
                     /translation="MSGTWACETSAGRKRPEAPDGVRNEFQRDRDRIIHAKAFRRLMH
                     KTQVFIAPDGDHFRTRLTHTLEMMQISRTIARSLGLNEDLTEAIALGHDLGHTPFGHT
                     GEAAISRALSAYGRTFHHNEHSLRVVDKLERGGAGLNLTLEVRDGILNHTGEGYPSTR
                     EGEIVRIADRIAYVNHDVDDALRAGIISWDELPEGPLSVLGEKMSVRIDTLVRDMISS
                     SEKRGEISLSEPVYEALMQLRAWLFRNVYGNPLSRENEKAAGVVAALFEYYLEHGGER
                     ATSDPDPIIETVDFVAGMTDRYALATYRRLFLPRGEIFA"
     gene            complement(1355..2107)
                     /gene="recO"
                     /locus_tag="VFT03_08390"
     CDS             complement(1355..2107)
                     /gene="recO"
                     /locus_tag="VFT03_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU4848202.1"
                     /translation="MAVYRSKGIVLRSIRYGEADRILDLYTRDAGLVSAIAKGIRRTR
                     SRFGARLEPLSCVDFVAYHGRTLDTVTQAEVLRSFHGVREDLARFEAAAGMVGNVRAL
                     SGGGEADRRVFNLLYNGLDALERRDSGFGSIEAAFGLKLSMLAGYAPQLDDCLDCGAS
                     LDEAAEPLYFAPNLGGVLCKECRTATADAFPMPPGTLDRLRAMIEIPIRESLPENGLG
                     EGVLRVVRSHVLAHAPGNALPKAPTPKRRRLA"
     gene            complement(2088..>2652)
                     /gene="deoC"
                     /locus_tag="VFT03_08395"
     CDS             complement(2088..>2652)
                     /gene="deoC"
                     /locus_tag="VFT03_08395"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU4848203.1"
                     /translation="RLAAQELRGVDVKVATVVSFPFGYDATAVKAAAVREAISSGAVE
                     LDVVMNISKFLSGQFDYVADELATLVQEVSVVAMNNGLNDVVVKVIVETAYLSDKMKR
                     LVTQIVAASGADFIKTSTGFGPAGATPEDVSVLREEAPEGLAVKASGGIRTLDDALDM
                     LNAGASRLGMSSSVAVMKEAEGGGLQE"
BASE COUNT          475 a          839 c          850 g          488 t
ORIGIN      
        1 tcgtcgactt catgcagatg acactggcga agagcgacct ccgcatagcc gagacctata
       61 cctcgctcgt ctcgaatagc gcagccagag aacgcctgtg gaataggatc tccgcggagc
      121 acgccgcctg tgtggaggcg ctgctcaaga tcacaggcca cgagagcctc ctcgacgaca
      181 gccctgtgtt gcagaggtcc atacgactgc gcaacccgta cgtcgatcct ctctcctacg
      241 tacaggtgag cctgctccga agactgcggg ctctgcccga agactccccc aagcgccaga
      301 cgacgctgaa tacccttctg ctcaccatct caggtatctc atccgggatg ctgaacacgg
      361 gttaacgcgc aggggcggtt cgtgtaaggg tggttcgcga atcgcccctg ctacgcgaag
      421 atctcacctc gcggcaggaa gagcctcctg tacgtagcga gggcataacg gtcggtcatg
      481 ccagctacga agtccacagt ctcgattatc gggtccgggt cgctcgtggc acgctccccg
      541 ccatgctcga ggtaatactc gaagagcgcg gctacgacgc cggcggcctt ctcgttctct
      601 cgcgagagcg ggttgccata aacgttgcgg aagagccatg ccctgagctg catgagcgcc
      661 tcgtagaccg gctccgagag agatatctca ccccgtttct cagagctgga gatcatgtcc
      721 cgtaccagcg tgtcgatcct gacgctcatc ttctccccga gcaccgagag cggaccctcc
      781 ggtaactcat cccaggagat gataccggcc cgcaacgcat catcgacgtc atggttcacg
      841 tacgcgatcc gatccgcgat ccgtacgatc tctccctcgc gagtcgacgg ataaccctcg
      901 ccagtatggt tgagtatccc atctctaacc tctaacgtaa ggtttaggcc tgctccaccc
      961 ctctcgagct tatccacgac gcgcagggaa tgttcgttgt ggtggaaagt tctcccgtaa
     1021 gctgagagtg cgcgggagat ggctgcttct ccggtatggc cgaacggtgt atggccaagg
     1081 tcgtggccga gggctatagc ttcggtgagg tcttcgttga ggccgagaga gcgggctatg
     1141 gtgcgtgaga tctgcatcat ctcgagtgtg tgggtcaagc gagtgcggaa gtgatctccg
     1201 tcgggagcga tgaagacctg ggtcttgtgc atgaggcggc ggaaggcctt ggcgtggatt
     1261 atgcggtccc tgtcacgctg gaactcatta cggacgccgt cgggggcttc gggcctcttg
     1321 cggccggcgc tcgtctcgca ggcccatgtg ccactcatgc caggcgtctg cgtttggggg
     1381 ttggggcttt gggaagggcg ttaccggggg cgtgagccag gacgtgggag cggacgaccc
     1441 gcaggacgcc ctcgccgagc ccgttctcag ggaggctctc gcggatcggg atctcgatca
     1501 tggccctgag tcgatccaac gttccggggg gcatcgggaa ggcgtcggct gtagcggtcc
     1561 tgcactcctt gcagagcacg ccgccgaggt tcggggcgaa gtacaggggc tcggcggcct
     1621 cgtcgaggct cgcgccgcag tccagacagt cgtcgagctg cggggcgtag ccggcgagca
     1681 tcgagagctt caggccgaag gccgcctcga tagaaccgaa gccggagtcg cggcgctcca
     1741 gggcgtcgag gccgttgtag aggaggttga atacgcgacg gtctgcctcg ccgcccccgg
     1801 agagggcgcg cacgttcccg accatgccgg ctgccgcctc gaagcgtgcg aggtcctcac
     1861 ggacaccgtg gaaactgcgc agcacctcgg cctgggtcac tgtgtcgagg gtacggccgt
     1921 ggtaggcgac gaagtccacg cacgagagcg gctcgaggcg ggcgccgaag cgagacctgg
     1981 tgcgccggat acccttagcg atggccgaga cgagccctgc gtctcgggtg tagaggtcga
     2041 ggatgcggtc agcctcgccg tagcggatcg aacgtaagac tatcccttta ctcctgtaga
     2101 ccgccaccct cggcctcctt catcacggcg acgctggagc tcatgccaag ccgcgaggca
     2161 ccggcgttca gcatgtcgag ggcgtcgtcc agcgtgcgga tcccaccaga cgccttgacc
     2221 gcgagtccct caggcgcctc ctccctgagc acggaaacgt cctcgggagt agctccggct
     2281 gggccgaaac cggtcgaggt cttgatgaag tccgcgccgc tcgcggccac gatctgggtt
     2341 accagtcgtt tcatcttgtc cgagaggtaa gccgtctcga cgatcacctt caccaccaca
     2401 tcgttgaggc cgttgttcat cgcgacgacg cttacctcct gcacgagggt ggctagctcg
     2461 tcggcgacgt agtcgaactg ccccgagagg aacttcgaga tgttcatgac cacgtccagc
     2521 tccacagccc cgctggagat agcctccctg acggccgccg ccttgaccgc cgtggcatcg
     2581 tagccgaagg ggaaggagac caccgtcgcg accttcacgt caaccccccg caactcctgg
     2641 gcggcgagcc gg
//
LOCUS       DASTUV010000416         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_177999, whole genome shotgun sequence.
ACCESSION   DASTUV010000416 DASTUV010000000
VERSION     DASTUV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177999"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..607
                     /locus_tag="VFT03_08400"
     CDS             80..607
                     /locus_tag="VFT03_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4848204.1"
                     /translation="MPPVRYAESIELLAPQTIAAHLATGVSEEDVEILARTGVAAAHC
                     PRSNNFLNCGVSPVPRMMERGVRVGMGTDGLWSSPSMNLFEETLFAVKLHGFDGATGL
                     ELATLGGARALGIEAETGSLEVGKWADLVVIETLPTSGDPEREVLEVAAGERGVAATV
                     VSGNPIYDRVQDTAG"
     gene            741..1292
                     /locus_tag="VFT03_08405"
     CDS             741..1292
                     /locus_tag="VFT03_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848205.1"
                     /translation="MNPRTSARFAWSVCGLTLVMIVCTLALFVVYGSLTVSLALVYLG
                     SVVVLQAIIRALTGQESQLAVVASTLAIAALFNPLRRRIQEFIDRRFYRGRYDAAKTL
                     DTFSARLGDETDLGTLSEDLLGVVRQTLQPAGASLWLRPTPKGEDPGERRPALVGPVQ
                     TVAGRQKWAAADIQSSDVRDAGG"
     gene            1299..1931
                     /locus_tag="VFT03_08410"
     CDS             1299..1931
                     /locus_tag="VFT03_08410"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4848206.1"
                     /translation="MMMNRDRIGFWVRLLSIVLAFFFVASFIFVGLGTNISYNLFDLI
                     GGDNQKAGEQTTDPQDQIQEAEKRLEEHPKDPDAIKELAALYYQSGRYDDAARVLQDG
                     REAAPEDPEIPFLLGQVYLQEAQATPGKEQDKLYKQAGDAFAAAAEIDPKNEDAYLFA
                     GQSYDRAGEPALAIKYYNGYLDLEPEGKQSEAVKERISALLEGGEETTSG"
     gene            2008..>2174
                     /gene="tatA"
                     /locus_tag="VFT03_08415"
     CDS             2008..>2174
                     /gene="tatA"
                     /locus_tag="VFT03_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4848207.1"
                     /translation="MPSIGGPELIIALVIILLLFGAKRIPELAKGLGTGMREFRKGSR
                     GEYEVKDEKEK"
BASE COUNT          421 a          636 c          752 g          365 t
ORIGIN      
        1 cgaggaggtg gagttcgtca gggaagggag cggcggcctc acagacatct tcggcagcaa
       61 caactggtga ggcgtgggag tgccgcccgt aaggtacgcc gaaagcatag agcttctcgc
      121 gccgcagacg atcgcggcgc acctggcaac cggtgtctcg gaggaggacg tcgagatcct
      181 ggcgcgcacc ggcgtggcag cggcccactg tcccaggtcg aacaacttcc tgaactgcgg
      241 cgtctcgccg gtgccacgga tgatggagcg cggcgtgcgg gtagggatgg gtaccgacgg
      301 cctctggagt agcccgagca tgaacctctt cgaggagacg ctcttcgccg tcaagctgca
      361 cggtttcgat ggtgcgacgg ggctcgagct ggcgacgctg ggcggggcgc gcgcgctggg
      421 catcgaggct gagaccggga gcctggaggt ggggaagtgg gctgatctcg tggtgatcga
      481 aacccttccg accagcggag accccgagcg agaggttctc gaggtcgccg ccggcgaacg
      541 aggcgttgcg gcgacggtcg tgagcggaaa ccctatctac gatagggtcc aggataccgc
      601 gggctgatcg cacgaagtcc ttgaagctcc tttcgtgagg gttctcgcaa ggctctgggg
      661 tggatcgttg tgtaaagtgg ttggcgttgt gatgccacgc gagcgagaga gtcgtagctc
      721 ttgagcgggg agccctccag atgaatcccc ggacctccgc ccggttcgcg tggtcggtgt
      781 gtgggctgac gctggtgatg atcgtctgca ccctcgcgct cttcgtcgtt tacggctcgc
      841 tcaccgtgtc gcttgcgctc gtctacctcg gcagcgtcgt ggtgctccag gcgatcattc
      901 gcgcgctgac cggtcaggag tcccagctgg ctgttgtggc ttccacgctc gccatcgccg
      961 cgctcttcaa ccctctgaga cgccgcattc aggagttcat agacaggcgt ttctaccgcg
     1021 ggaggtacga cgcggcgaag accctcgata ccttctcggc caggctgggg gacgagacgg
     1081 atctgggcac cctgagcgag gacctgctcg gggtggtacg acagacgttg cagccggccg
     1141 gcgcatcgct gtggctgcgt cccacaccaa agggcgagga ccccggcgaa cggaggccag
     1201 cgctggtcgg accggtacaa accgtcgccg gtcgacaaaa gtgggctgcg gctgatatac
     1261 aatcgagcga cgttcgagac gccggaggat agcctgacat gatgatgaac cgggacagga
     1321 tcggcttctg ggtgaggctg ctctcgatcg tgctcgcctt cttcttcgta gcctccttca
     1381 tcttcgtggg cctcggcacc aacatcagct ataacctgtt cgacctgatc ggaggcgata
     1441 accagaaggc cggagagcag acgaccgatc cccaggacca gatccaggag gccgaaaaac
     1501 gactcgaaga gcacccgaag gatcctgacg ccataaagga gctcgcggct ctctactacc
     1561 agtccggcag gtacgacgac gcggcccggg tgctgcaaga cggacgcgag gccgctcccg
     1621 aagaccctga gataccgttc ctgctcgggc aggtctacct ccaggaggcc caggccacgc
     1681 cgggcaagga gcaggacaag ctctacaagc aggccggcga cgcgttcgcg gcggccgcag
     1741 agatcgatcc caagaacgag gacgcgtacc tgttcgcggg ccagtcctac gacagggcgg
     1801 gcgagccggc cctggccatc aagtactaca acggctacct ggacctcgag ccggaaggca
     1861 agcagtcgga ggcggtcaag gagcgcatct cagcgctgct cgagggcggc gaggagacga
     1921 ccagcgggta ggcgtgatcc ggttttcgaa tcaaggtcgt ataatgtggc gtaatatcct
     1981 ggagaccgtc agttaggagg agagacaatg ccgagcatag ggggaccgga gctaattatt
     2041 gccctcgtca tcatcctttt gctcttcggc gcgaagcgca tcccggaact cgccaaaggg
     2101 ctcgggaccg gtatgcgcga gttcaggaag ggctcgcgcg gcgagtacga ggtcaaggac
     2161 gagaaagaga aaga
//
LOCUS       DASTUV010000417         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_370759, whole genome shotgun sequence.
ACCESSION   DASTUV010000417 DASTUV010000000
VERSION     DASTUV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370759"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFT03_08420"
     CDS             <1..513
                     /locus_tag="VFT03_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEU4848208.1"
                     /translation="DSKYLSPGLLADAFGNIPGEMIDTGNRMLKPVTNYVGTYVSMWE
                     RIFDEKPMETWLAMNKWVNDGPPFPGAAFRQWITDFYQHNKLVKGEIYLRGRRVDLSN
                     IKVPLLNIAGKKDHICTLPQAEATMDLVSSEDKEFFVLDAGHVGLMTGRGAKKGLWPK
                     LSGWLEERSG"
     gene            908..>3276
                     /locus_tag="VFT03_08425"
     CDS             908..>3276
                     /locus_tag="VFT03_08425"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4848209.1"
                     /translation="MEPLLSTKLHPSLARPKLVARPRLIASLTREPGRRLTLLSAPAG
                     FGKTTLLSEWARERAGSVAWVSLDEGDNDPIRFVSYLVAALRGTGEEGFGEAVLAALR
                     SPEPPRMEAVLSTLVNELAALPGEVAVVLDDYHQIDSESVHRIVSFLLERLPENTHLV
                     ISGRVDPPLPLTRLRARDQMTELHAADLRFTPEEAAAFLSDVMSLDLSADDVTALEGV
                     TEGWIAALQLAALSMRKREDVSGFIRSFSGGHRDVFDFLAEEVLGGQTEPVQSFLLET
                     SILDNLSGPLCDALTGRDDGQETLERLERENLLVVPLDDERRWYRYHHLFADFLRGRL
                     TRERPEQLATLHLRASEWCEQNALGIEAVRHALAAGDHERAARLMESGVGQTWYRGEV
                     VTLLGWLQKLPGEAMRDRPLLLVWYAAALMLAGRLDAVESLLGEADRALGGAQGEEPR
                     PDGGDHSRLLATAAAVRSMHARFRGDSQGAIEHARRALTLLPEDNLDPRPFAAIALAQ
                     AYQAAGDLEAAIAAFAEAGALGQAAGHDYIALSAMASRAHLQLARGRLREADEVLRSA
                     LGFAAEREAELLPALGSVRIATGELCYEWNEVDAAARHLTEGVGLAGRTGDVGILTWG
                     HIVLSRVRLAQGDAEGAFAAAREAELVAQSSGADEAIVDAAVWKARLHLASGDLESAS
                     SEQERAARVGKVRPQSRELERLLLARLLVARNEPREALQLLAQLREAAETAGTTVQIL
                     ALQALALQAKGERERAVRTLAEALVLAEPEGYVRTFADEGPPMNELLSQA"
BASE COUNT          559 a         1058 c         1155 g          504 t
ORIGIN      
        1 gacagcaagt acttgagccc ggggctgctg gccgacgcgt tcggcaacat ccccggcgag
       61 atgatcgaca cgggcaacag gatgctcaag ccggtcacca actacgtcgg tacctacgtc
      121 agcatgtggg agcgcatctt cgacgagaag ccgatggaga cctggctcgc gatgaacaag
      181 tgggtcaacg acgggccgcc cttccccggc gcggccttcc ggcagtggat caccgacttc
      241 taccagcaca acaagctggt gaagggtgag atctacctcc gcggccgccg cgtggacctc
      301 tcgaatataa aggtcccgct ccttaacatc gcgggcaaga aggatcatat ctgcaccctc
      361 ccgcaggcag aggccaccat ggacctcgtc agcagcgagg acaaggaatt cttcgtcctc
      421 gacgccggcc acgtcggcct tatgacgggc cgcggcgcca agaagggcct gtggcccaag
      481 ctcagcggtt ggctcgagga gcgctccggc tagcgattca gcacgccgcc tccctccggt
      541 cggcggcttg tcagcacgca tcgggccttt agaaggccct cgcttgtcag catttcagct
      601 tctctttgtg ctgagagaag ctgagaagcc gcctggcgaa gccaggtggc gtgctgagag
      661 cgaggcccgg agggccggag cgtgctgaaa gcctgcgaag caggcgtgct tacaagcgga
      721 ggcgtcagcc ggagcgtgct gcttccaacg gggtgaggat cgctccagca gaaagacgcc
      781 ccgtgagttg gcagatccgg agtagtacga tcgatactca gagtctttga tccttcaata
      841 gacgggcgaa gctattaaca tgggtgccac gatgagtcgg tgatcggaag gcgaggtagc
      901 cagcgcattg gagccgctcc tatcgaccaa gctgcatccc tccctggcgc gtccgaagct
      961 tgtggcgagg ccgcgcctga tcgcgagcct gacgcgggag ccggggcgca ggctgaccct
     1021 tctctccgct cctgcgggct tcggaaagac caccctcctg agcgagtggg ccagagaacg
     1081 tgcgggctcc gtcgcctggg tttcgctgga cgaaggggac aacgacccca tccggttcgt
     1141 gtcctatctc gtcgccgcgc tcaggggtac cggcgaggag gggttcggcg aggccgtcct
     1201 ggccgcgctg cgctcgcccg agccgccgcg gatggaggcg gtgctgagca ccctcgtcaa
     1261 cgagctggcc gctctcccag gggaggtcgc ggtggtcctc gacgactacc accagataga
     1321 ttccgagagc gtacacagga tcgtctcgtt cctgttggag cgattgccgg agaacacgca
     1381 cctcgtgata tccggccgtg tcgacccgcc cctgcccctc accaggctgc gcgcccggga
     1441 tcagatgacc gagctgcacg ccgctgacct gcgctttaca ccggaggaag cggcggcctt
     1501 cttgagcgac gtgatgagcc tggacctctc ggccgatgac gtcacggcgc tggaaggggt
     1561 cacggagggt tggatcgcgg ccctgcaact ggccgcgctc tcgatgagga agcgcgagga
     1621 cgtctcgggc ttcatccggt ccttctcggg cggtcaccgc gacgtcttcg acttcctggc
     1681 cgaggaagtg ctgggggggc agaccgaacc ggtgcagtcg ttcttgctgg agacctcgat
     1741 cctggacaac ctgagcggcc cgttgtgcga cgctctcacg ggccgcgacg acggccagga
     1801 gactctggag cggctggaga gggagaactt gctcgtcgtc ccgctggacg acgagcgacg
     1861 ctggtaccgt taccaccacc tcttcgccga cttcctgcgc gggcgtctta cgcgcgagcg
     1921 acccgagcag ctggcgacgt tgcatctcag ggcctccgag tggtgcgagc agaacgcgct
     1981 tggtatcgag gcggtcaggc acgcgctcgc cgccggcgac cacgaaaggg ccgcgcggct
     2041 gatggagagt ggcgtcggac agacctggta ccgcggcgag gtggtaacgc ttctgggctg
     2101 gctccagaag ctgccaggag aggcgatgcg cgaccggccg ctgctgctag tctggtacgc
     2161 tgccgcgctg atgctcgcgg gccggttgga cgccgtggaa tccctcctgg gagaagccga
     2221 ccgtgcgctc ggcggagccc agggtgagga gccgcgaccc gacgggggcg accactcgcg
     2281 tctgctggca accgccgcgg ccgtccgatc catgcacgcc cgcttcagag gggactcgca
     2341 gggggccatc gagcacgcca ggcgggccct cacgctcctg cccgaagaca acctggaccc
     2401 gcgtcccttc gccgccatcg ccctggcgca ggcctaccag gcggccggcg acctcgaggc
     2461 ggctatcgct gccttcgccg aggcgggggc tcttggtcag gccgccggcc acgattacat
     2521 agcgctgagc gccatggcat cccgggccca cctgcaactg gcaaggggcc ggctacgcga
     2581 agcggacgag gttctgcgga gcgccctcgg gttcgcagcc gagcgggaag ccgagctgtt
     2641 gccggccctg gggagcgtgc gcatcgcgac gggcgagctc tgctacgagt ggaacgaggt
     2701 ggacgccgcg gcgcgccacc tgacggaggg cgtggggctc gcggggcgaa cgggagacgt
     2761 cgggatcctc acgtggggac atatagtcct ctcgagggta aggctcgcgc agggcgacgc
     2821 ggagggcgcc ttcgcggcgg ccagggaagc ggagctggtg gcccagagct cgggcgccga
     2881 cgaagcgatc gtcgacgcgg cggtctggaa ggcgaggctg cacctggcaa gtggggatct
     2941 ggaatccgcc tcctccgagc aggagcgtgc ggcgcgcgtg ggcaaagttc ggccccagtc
     3001 ccgcgagctg gagcgcctct tgctggcacg gctgctggtc gcccgaaacg agcctcgcga
     3061 agcgctgcag ctgctggcgc agctgcgcga ggcggcagag accgcgggca cgactgtaca
     3121 gatactggcg ctgcaagcgc tggccctgca ggcaaaaggc gagagagaac gagccgtgcg
     3181 caccctggct gaagccctcg ttctggccga accggagggc tacgtcagga ccttcgccga
     3241 cgagggacct ccgatgaacg aactcctctc ccaagc
//
LOCUS       DASTUV010000418         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_15230, whole genome shotgun sequence.
ACCESSION   DASTUV010000418 DASTUV010000000
VERSION     DASTUV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15230"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFT03_08430"
     CDS             complement(<1..212)
                     /locus_tag="VFT03_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848210.1"
                     /translation="MSHSPRAHSCVVHPELKNTRLSLGGKYGRKFPGLLPCEVDLDSA
                     VGLGRAASRMDATLPVVDEALENARIP"
     gene            complement(322..588)
                     /locus_tag="VFT03_08435"
     CDS             complement(322..588)
                     /locus_tag="VFT03_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848211.1"
                     /translation="MASESEDTRRQLLRAAYAVAARKGGPSASVHLHEVAKEMELKDP
                     GRDEDVRNELTSTVLELQEEGEVEGWSPTNARFRLTSQGASKAE"
     gene            complement(677..889)
                     /locus_tag="VFT03_08440"
     CDS             complement(677..889)
                     /locus_tag="VFT03_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848212.1"
                     /translation="MSERRDARNEKERQQGELEDAGWELVERGSGKTIWRNPRSGRLY
                     PQYVAITMVRDVPSPKNSDRDPGDRA"
     gene            complement(1050..1334)
                     /locus_tag="VFT03_08445"
     CDS             complement(1050..1334)
                     /locus_tag="VFT03_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848213.1"
                     /translation="MSELDLAGRLEDLFRETGEAHHQAYIETDGADPEWPLWYAGYLQ
                     ERLGPLLEASFTRSELVYLLIWVANEQPLYAPGADWARYYADFFLQRYAV"
     gene            1492..>2637
                     /locus_tag="VFT03_08450"
     CDS             1492..>2637
                     /locus_tag="VFT03_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU4848214.1"
                     /translation="MSPDNGGKKDLRRLNHELSVLNEIARELNRSVNLGEALGFTLAQ
                     VAELLGLRTGWIWLMNEDSSEPYLAAVQNLPPALADDPRRMDGSGYCYCLDTYKRGDL
                     EGAANVNVLTCSRLGGLVDGTDGLRYHASIPLYAQEKKLGVMNVASPGWRSLSPEDLQ
                     LLYTIGDLLSIAIERAELFARSTRLGAVEERNRLAREIHDTLAQGLTATALQLESADA
                     LLDAGSEKAHEPLRRALSLTRSNLEEARRSVLDLRASPLEGRPLSEALKALVERWEAE
                     TGIAARYGAVNGSRPLPPSVEAALYRICQEALTNVARHAGAERATVRLVATPDRVRLA
                     VEDDGRGFDAAGVPEDRHGIVGMRERAEVLGGTLEASSGPGEGTRIEV"
BASE COUNT          430 a          916 c          840 g          451 t
ORIGIN      
        1 gggatgcgcg cgttttcgag cgcctcgtca acgaccggga gggtggcgtc catgcgcgag
       61 gccgcccgcc cgagcccgac cgcgctgtcc aggtcaacct cgcaggggag gagcccggga
      121 aacttcctgc cgtatttccc accgagggag agtcgggtgt tcttgagctc cgggtgtact
      181 acgcagctgt gggctctggg gctgtgggac accgggatct cctttcgctt gcaaggttgg
      241 atccactcta caaaggcggc gaagcctgcg catcacccga acggctaggt ttcgcccggt
      301 ccccgcttcg agcgcggagc tctactcagc cttgctcgcg ccctgagagg tgagcctgaa
      361 gcgcgcgttg gtgggagacc acccctccac ttcgccctcc tcctgaagct cgagcaccgt
      421 gctcgtgagc tcgttccgga cgtcttcgtc cctgccgggg tccttcagct ccatctcctt
      481 cgcgacctcg tgcaggtgca ccgaggcaga aggtcctcct ttgcgcgcgg ccacggcata
      541 agccgcccgc agaagttgcc ggcgagtgtc ctccgattcg ctggccatat tcgcgcccct
      601 cctttctaac gcttccccac gttatccgtc actctaacgt tccgaagcgc ccgtcgctac
      661 gaggtgagga tcagcattac gccctgtccc cagggtccct atcggagttc ttcgggctcg
      721 gtacgtccct caccattgtt atggcgacgt actgtgggta caggcggccg ctcctggggt
      781 tccgccagat ggtcttgccg cttccgcgct cgaccagctc ccacccggcg tcctctagct
      841 ctccctgctg ccgttccttc tcgttgcgcg cgtcgcgcct ctcgctcatg atgtccctcc
      901 ttcggtgcag cgacgaagca cggctcggcc cagggatcca tgccggcgct gtcgtcgcgc
      961 ggtatcgtgg gtctctctat acgcctgcgt acccaccgat tgtaaccgcc gagaggcgcc
     1021 acttcgcgca gagatcaggc cgcagcccgt taaactgcgt atctctgcaa aaagaaatcc
     1081 gcgtagtaac gcgcccagtc cgcgcccggg gcgtagagcg gctgctcgtt cgcaacccag
     1141 atcaggaggt agacgagctc gctcctggtg aagctggcct ccaggagagg gccgagcctc
     1201 tcctggaggt acccggcgta ccacagcggc cactcggggt cggcgccgtc cgtctcgatg
     1261 tatgcctggt ggtgggcctc tccggtctcg cgaaacaggt cctccagccg cccggcgaga
     1321 tccagctcgc tcacgccatc ctcctcagca gcttgaatct tgcattactt ttcacactat
     1381 agccgcgtgt gccgagggcg cgcaacgccg gattggctcg gcgccgtcgg acgccgtgcc
     1441 tggcctctcc cacgctgata gactcatctc caaggaggta aagcaaaggc gatgtccccg
     1501 gacaacggcg ggaagaagga cctgcggcgc ctgaaccacg agctttcggt gctcaacgag
     1561 atcgcgcgcg agctgaaccg ctctgtgaac ctgggcgagg cactgggttt cacgctggcc
     1621 caggtcgcgg agctcctcgg cctccggacg ggttggatct ggctcatgaa cgaggactct
     1681 tccgagccct atctggcggc cgtccagaac ctcccgccag cactagcaga cgatccccgc
     1741 cgcatggacg ggtcggggta ttgctactgc ctcgacacgt acaagagagg cgacctcgaa
     1801 ggggcggcga acgtcaacgt gctcacatgt agccgactcg gcgggctcgt cgacgggact
     1861 gacggcctcc gctaccacgc cagcatcccg ctctacgctc aagaaaagaa gctcggcgtc
     1921 atgaacgtcg ccagccccgg ctggcggagc ctctccccgg aagacctgca gctcctctac
     1981 accattggcg acctgttgag catcgctata gagagggccg aactcttcgc gcggagcacc
     2041 aggctcgggg cggtcgagga gcgtaacagg ctggcgcgcg agatccacga caccctggcc
     2101 caaggcctca cagctacagc gctgcagctg gagtccgccg acgcgctgct cgacgcgggc
     2161 tcggagaagg cgcacgaacc gctgcgccgc gcgctatccc tcacccgctc caacctcgaa
     2221 gaggccaggc gatcggtgct tgacctgcgt gcctccccgc tcgaaggacg gccgctctcc
     2281 gaggcgctca aggccctcgt cgagcggtgg gaagccgaga cgggcatagc cgcgcgctac
     2341 ggggcggtga acggcagcag gccgctcccg cctagcgtgg aggcggccct ttaccgcatc
     2401 tgccaggagg cgctcacgaa cgtggcccgg cacgcggggg cggagcgcgc taccgtccga
     2461 ctcgtcgcca cccccgacag ggtgcggcta gcggtagagg acgacggcag gggcttcgac
     2521 gccgcaggag tacccgaaga ccggcacgga atcgtgggga tgcgcgagcg ggcggaggtg
     2581 ctcggcggca ccctcgaggc cagcagcggg cccggcgagg gaacccggat cgaagtc
//
LOCUS       DASTUV010000419         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1511713, whole genome shotgun sequence.
ACCESSION   DASTUV010000419 DASTUV010000000
VERSION     DASTUV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511713"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFT03_08455"
     CDS             <1..862
                     /locus_tag="VFT03_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU4848215.1"
                     /translation="PFSHSGPLSRTVEDAALMLGAMAGPHPRDPLSLPDEGIDYVAAT
                     RLPVEGMRIAYSPDLGVFPVEERVLSVIEEAVRAFEEAGARVEEANIEFGRGQRELCE
                     LWLREVAVKSAEIVSNLEDAGVADLLGEHRENLTPEFAALLDMGHAMGAVEYRKDEVA
                     RTEVFDAIQDLFDDYDLLVTSTLAVPPFDNAGDGNTLGPSRVNGEEVDPLIGWCLTYP
                     INFTGHPAASVPAGFTAEGLPVGMQIVGRRFDDGAVLAASAAFERLRPWHDSYDALAT
                     SGASNVGKVG"
     gene            865..2334
                     /locus_tag="VFT03_08460"
     CDS             865..2334
                     /locus_tag="VFT03_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEU4848216.1"
                     /translation="METRKPARQRDTQDRRREGIVSGVEGFNERGRRAVRDPELHDSV
                     SSFVNKTLKGREATLAALPEAPELRDRAYHIKQETMANLDRYLEQMADAVEARGGNVF
                     FAESGEDVVRYIGELARRRGTKVITKSKSMATEEIELNRRLEEDYSDLGLEIVETDLG
                     EWIAQLAGDHPSHIVGPILHMNARRVAEILSEAAGEELPPDAQQLTNFARGRLREKFL
                     AADIGITGANFGIAETGTIVTVTNEGNARLVTSVPPVHVVVMGIEKLLPRFEDLSVFV
                     QLLSRNGTGQKITVYTNFVTGPRGEGELDGAEEMHLILMDNGRSKLLGTEFEEALYCI
                     RCGSCLNVCPVYRQTGGHAYGSTYSGPIGAVITPLLKGNEQAKDLPHASSLCGACWET
                     CPVGIPLHDLLLKLRNRQVEEGLAGRAQSLMFKGFEKTMKSPALYKAGAKAGRVAQKP
                     VVRDGSVRPLPGPMSAWTNTRDLPPLAEKSFRELWKEGI"
     gene            2337..>2614
                     /locus_tag="VFT03_08465"
     CDS             2337..>2614
                     /locus_tag="VFT03_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848217.1"
                     /translation="MADRAGFLDNIRHRTRSGQYKPTNAPDAAWTRSPERPFTSERIE
                     DPPSRFLEELDALGGHGERVANMDEARDYVVALARERGAKLLVRWDVE"
BASE COUNT          464 a          873 c          914 g          363 t
ORIGIN      
        1 gccgttctcc cacagcgggc ccctgagccg gacggtcgag gacgccgctc tcatgctcgg
       61 cgcgatggcc ggtccccacc cgcgcgaccc gcttagcctc cccgacgagg ggatagacta
      121 cgtcgccgcg acccgcctgc ccgtcgaggg gatgaggatc gcctacagcc cggacctggg
      181 cgtcttcccc gtcgaggaaa gggtgctctc cgtgatcgaa gaggccgtgc gcgcgttcga
      241 ggaggccggg gctcgtgtgg aggaggcgaa catcgagttc gggcgcggcc agcgggagct
      301 gtgcgagctc tggctgagag aggtcgctgt gaagtcggcg gagatcgtct ccaacctcga
      361 ggacgccggc gtcgccgacc tgctcggcga gcacagggag aacctgacgc cggagttcgc
      421 cgcgctgctg gacatggggc acgcgatggg ggccgtcgag tatcgcaagg acgaggtcgc
      481 caggacggag gtcttcgacg cgatccagga cctcttcgac gattacgatt tgctcgtgac
      541 gtcgaccctc gccgtgccgc ccttcgacaa cgccggcgac ggtaacacgc tgggaccctc
      601 aagggtaaac ggcgaggagg tcgacccgct catcgggtgg tgcctgacgt acccgatcaa
      661 cttcacgggc caccctgcgg cctccgtgcc agcggggttc accgccgaag ggttgcctgt
      721 cgggatgcag atcgtgggcc gcaggttcga cgacggggcg gtgctcgccg cgagcgccgc
      781 cttcgagcgc ctcaggccct ggcacgactc atacgacgcg ctggcgacga gcggagcttc
      841 gaacgttgga aaggtcgggt agcgatggag acgaggaaac cggcgcggca gcgcgacacg
      901 caagacaggc gccgcgaggg gatagtaagc ggggtcgagg gcttcaacga gcgcgggcgg
      961 agggccgtgc gggaccccga gctgcacgac tcggtctcct cgttcgtcaa caagaccttg
     1021 aaggggcgcg aggcgacgct cgcggcgctc ccagaggcgc cggagctgcg cgatagggcc
     1081 tatcacatca agcaggagac gatggcgaac ctcgaccgct acctcgagca gatggccgac
     1141 gccgtcgagg cgcgcggggg caacgtcttc ttcgcagaga gcggcgagga cgtggtccgc
     1201 tacatcgggg agctcgccag gcgcaggggc acgaaggtca taaccaagtc caagagcatg
     1261 gccacggagg agattgagct caaccgcagg ctcgaggagg actactccga cctggggctc
     1321 gagatcgtgg agacggacct gggggagtgg atcgcgcagc tcgcgggcga ccatccctcg
     1381 cacatagtcg gccctatcct gcacatgaac gcccgccggg tcgcggagat actctcggag
     1441 gccgcggggg aggagctgcc gcccgacgcc cagcagctga ccaacttcgc ccgcggccgc
     1501 ctgcgcgaga agttcctggc ggctgacatc ggcataacgg gcgccaactt cgggatcgcc
     1561 gagacaggaa ccatcgtgac cgtcaccaac gagggaaacg ccaggctggt gaccagtgtt
     1621 ccccccgtcc acgtcgtcgt gatgggcata gagaagctcc tcccgcgctt cgaggacctc
     1681 tcggtcttcg tgcagctact ctcgaggaac gggacggggc agaagatcac cgtctacacc
     1741 aacttcgtca cagggccccg cggcgagggc gagctcgacg gcgccgagga gatgcacctg
     1801 atactcatgg acaacggccg ctcgaagctg ctcggcacgg agttcgagga ggcgctctac
     1861 tgcatccgct gcggctcctg cctcaacgtc tgccccgtct accgccagac gggcggccac
     1921 gcctacggct cgacgtactc gggcccgatc ggggccgtca tcacgccgct cctgaaaggc
     1981 aacgaacagg ctaaggatct gccccacgcc tcctcccttt gcggcgcgtg ctgggagacc
     2041 tgtcccgtcg gcatcccgct ccacgacctc ctcctcaagc tccggaaccg ccaggtcgag
     2101 gaggggctcg cgggccgggc gcagagcctg atgttcaagg gcttcgagaa gaccatgaaa
     2161 agtcccgctc tctacaaggc cggcgcgaag gccggcaggg tagcgcagaa gccggtcgtg
     2221 cgggacggct ccgtgaggcc gctcccgggc cccatgagcg cctggaccaa cacccgcgac
     2281 ctgccgccgc tggccgagaa gtccttccgc gagctgtgga aggaggggat ctgaggatgg
     2341 ccgaccgcgc cgggttcctg gacaatatcc gccaccgcac ccgctcgggc cagtacaagc
     2401 cgaccaacgc cccggacgcc gcctggaccc gctcgccgga gaggcccttc acgagcgagc
     2461 gcatcgaaga ccccccgtct cgcttcctcg aagagctgga tgctctaggc ggccatggcg
     2521 agcgggtcgc caatatggac gaggcgcggg actacgtcgt cgccctcgcg cgcgagcgcg
     2581 gggcgaagct cctcgtccgc tgggacgtgg agga
//
LOCUS       DASTUV010000420         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_783939, whole genome shotgun sequence.
ACCESSION   DASTUV010000420 DASTUV010000000
VERSION     DASTUV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783939"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFT03_08470"
     CDS             <1..976
                     /locus_tag="VFT03_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU4848218.1"
                     /translation="AISLQERAGLPVVTDGEMRRESFQSQMVEAVEGFGEHTIDAWLW
                     GDWHGDETVGDKKIERPRTLGVVGKLRRRRFLSAEEFVYARARTERTIKVTLPSPSLY
                     ANLWSPELSGEVYPTLDDFLDDVAEILRDEVEELVRLGATYIQLDAPHYPLLLDPSTR
                     EFYEGRGWDFDRWMSRGVELDNYVIGENPGVTFAFHLCRGNQGSRWLVSGSYEPLARR
                     IFPRLRAGRLMLEYDDERSGGFEPLAHVPEDKVAVLGLVTTKTGRRETPEELETRIRE
                     ASRFVPLERLALSPQCGFATSIIGNALSVEDEERKLRTIAETAERVWG"
     gene            1053..1715
                     /locus_tag="VFT03_08475"
     CDS             1053..1715
                     /locus_tag="VFT03_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HEU4848219.1"
                     /translation="MLRVRDSMTREVVTLGPEASAAEAWSVCQDLGIRHLPIVEAGRL
                     VGLVSDRDLRDVSPPRGSGGERDTLGWVRLRDIMSTDLVTIEPLDTIEHAAREIYDRK
                     IGCLPVVAEGELVGIITSSDMMRTLIELIGAHGIGSWVEVEVPNQPGMLANVTDVIRA
                     RQVNIAGVFLAPAPRASNRLIVLRLETTNPAGTVQSLEEAGYKVTAVKSSVPVEPTLE
                     EA"
     gene            complement(1759..2646)
                     /locus_tag="VFT03_08480"
     CDS             complement(1759..2646)
                     /locus_tag="VFT03_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4848220.1"
                     /translation="MKVLVTGARGKVGRATVAALMEAGHDVRATDLAPPVFERPEPGE
                     PAYVQADLSDAGDAFAVVRGAEAVVHAAAIPEPTQNPPHVVFHNNLMSVFNVLEAAIR
                     FGAERFVNISSETVPGFFFAERPFLPDYIQVDEEHPIRPQDPYATAKHFSELLMDGAV
                     RRSDIRPISIRPCWVHHEGNYERNLGPLVRELPPDLSPNFLSYIDVYDLADAIVLATQ
                     SDLSGHEVFYIASPDNVGNRPLEEVVRRYYGDEIEIRKPLPREDASYISIEKAQQLLG
                     YDPKRSWSDYLDEEGRRKL"
     gene            2744..>3471
                     /locus_tag="VFT03_08485"
     CDS             2744..>3471
                     /locus_tag="VFT03_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4848221.1"
                     /translation="MRVVVVGATGNVGTSVLRSLEKEERVESVLGLARRLTGLTMPKV
                     EWASADVVSDDLVPHFRGADAVVLLAWIIQPSRDLNKQWMVNVEGSTRAARAAKEAGV
                     PALHYASSVGAYSPGPKDRRVDESWPTGGTPTSYYARHKAEVERRLDRFEQEAPDVRV
                     VRMRPGLVFKKEAATGIRRLFAGPFLPGPLVRPELIRVVPDVPNLRSQIVHSYDVGEA
                     FRLAIVNDEARGSFNLATEPVLDPA"
BASE COUNT          593 a         1084 c         1218 g          576 t
ORIGIN      
        1 ggcgatctcg ctccaggagc gcgccggcct ccctgtagtc accgacggag agatgcgccg
       61 cgagtccttc cagagccaga tggtggaggc ggtcgagggt ttcggggagc acaccataga
      121 cgcctggctc tggggggact ggcacggcga cgagacggtg ggcgacaaga agatcgaacg
      181 cccccggacg ctcggcgtgg tcggcaagct gcgccgcagg cgcttcctct cggccgagga
      241 gttcgtctac gcccgcgccc gcacggagcg cacgatcaag gtgacgctcc cgagccccag
      301 cctctacgcc aacctctggt cgccggagct ctccggggag gtctacccca ccctcgacga
      361 tttcctcgat gacgtggcgg agatcctgcg cgatgaggtc gaggagctgg tgcggctcgg
      421 tgcgacctac atccagctcg acgcgccgca ctacccgctg ctccttgacc cctcgacccg
      481 cgagttctac gagggaagag gctgggactt cgaccgctgg atgagccgcg gcgtagagct
      541 cgacaactat gttatagggg agaatcccgg cgtcacgttc gccttccacc tgtgccgcgg
      601 caaccagggg agccgctggc tcgtctcggg ctcctacgag ccgctggcgc gcaggatctt
      661 cccgcgtctg cgcgccggga ggctgatgct cgaatacgac gacgagcgct ccggcggctt
      721 cgagcccctt gctcacgtgc cggaggacaa ggtcgccgtc ctcggcctcg tcacgacgaa
      781 gaccggccgg cgcgagacac cggaggaact ggagacccgc atcagggaag catccagatt
      841 cgtgccgctg gagcggctgg cgctgagccc gcagtgcggg ttcgccacat cgatcatcgg
      901 caacgctttg agcgtcgagg acgaggagcg caagctccgc actatcgccg agaccgcgga
      961 gcgcgtttgg ggttagcggc ggcgggagac ctataccggg atcgggcagg atggtaagct
     1021 gccggggccg attactttag aggaggaagc ggatgttgcg ggtaagggac tccatgacga
     1081 gggaggtcgt cacgctgggg ccggaggcga gcgccgccga ggcttggagt gtctgtcagg
     1141 atctcgggat aagacacctg cccatcgtcg aagcggggcg ccttgtgggg ctggtctccg
     1201 atagggatct gagggacgtg agcccgccgc ggggctccgg cggcgagagg gacacgctcg
     1261 ggtgggtgcg cctgagggac ataatgagca cggatctggt caccatcgag ccactcgaca
     1321 ccatagagca cgcggcgcgc gagatctacg accgcaagat agggtgcctc cccgtggtgg
     1381 ccgagggcga gctggtgggg atcattacct cctctgacat gatgcgcacc ctgatagagc
     1441 tcatcggcgc gcacgggatc gggagctggg tcgaggtcga ggtgccgaat cagccgggca
     1501 tgctcgccaa cgtgaccgac gtgatccggg cccggcaggt caacattgcc ggcgtcttcc
     1561 tggcgccagc gccgcgagcg agcaaccgcc tgatcgtgct ccgcctcgag accactaacc
     1621 ccgccgggac tgtccagagc ctcgaagaag ccggctacaa ggtgacggcc gtaaagtcgt
     1681 cagtccccgt ggagccaacg ctcgaggagg cctaggaagg caccaggtaa aacccacaag
     1741 ctgtagcctc ctgtagcctt agagcttgcg tcttccttct tcgtcgaggt agtcgctcca
     1801 ggagcgcttc gggtcgtagc cgagcaactg ctgcgccttc tctatggaga tgtagctcgc
     1861 gtcctcgcgc gggaggggtt tcctgatctc gatctcatcg ccgtagtacc ggcggacgac
     1921 ctcttcgagc ggtcggttcc cgacgttgtc gggagaggct atatagaaga cctcgtgacc
     1981 cgagaggtcg ctctgagtgg cgagcacgat ggcgtcggcc aggtcgtaga cgtctatgta
     2041 gctcaggaag ttgggggaga ggtccggcgg tagctccctt accaacggtc ccaggttgcg
     2101 ctcgtagtta ccctcgtggt gcacccagca gggccttatg gagatgggcc ggatgtcaga
     2161 gcgcctcacg gccccgtcca tgagcagctc cgagaagtgc ttggctgtgg cgtatgggtc
     2221 ctggggcctt atgggatgct cttcgtcgac ctggatgtag tcgggcaaga agggtcgctc
     2281 ggcgaagaag aagccgggca ccgtctcgct cgagatgttg acgaaccgct ctgcgccgaa
     2341 cctgatggcg gcctccagca cgttgaagac ggacatcagg ttgttatgga agacgacgtg
     2401 cggcgggttc tgggtcggct ccgggatggc cgcggcgtgc acgaccgcct ccgcgccccg
     2461 gacgaccgcg aaggcgtcgc ctgcatcgct cagatcggcc tgaacgtagg cgggctcgcc
     2521 gggctcggga cgctcgaaga ccggtggcgc caggtcggtc gcgcgcacgt cgtggccggc
     2581 ctccatgagc gccgccaccg tcgcccttcc caccttgccc cgcgctcccg tcaccagtac
     2641 cttcatagag tcctcctcct ggatgcgaac cggcacagat ggtacagcat agccctcggc
     2701 ggcgagagtt tcgtgccgga tagtcggggg taaaagggcc agcatgagag tagtagtggt
     2761 aggggcgacc ggtaacgtag ggacaagcgt gctccggtcg ctcgagaagg aagagagagt
     2821 cgagtccgtc ctcgggctgg cgcgccggct tactggcttg acgatgccca aggtcgagtg
     2881 ggcctcggcc gacgtggtga gtgatgacct ggtgccgcac ttccgagggg ccgacgcggt
     2941 ggtgctgctc gcctggatca tccagccctc tcgcgacctg aacaagcagt ggatggtgaa
     3001 cgtcgagggg agcacgcggg cggcgagggc cgcgaaggag gctggggtgc cggcgctcca
     3061 ctacgcttcg tcggtaggcg cgtactcgcc tggcccgaag gaccggcgcg tggacgagag
     3121 ctggccgacg ggcggtacgc cgacctccta ctacgcgcgc cacaaggccg aggtcgagcg
     3181 caggctggac cgcttcgaac aagaagcgcc ggatgtccgg gtcgtcagga tgcgccccgg
     3241 cctcgtcttc aagaaggagg cggcgacggg gatacggcgc ctcttcgccg gtccttttct
     3301 gcccggtcct ctcgtccggc cggaactcat aagagtcgtc cccgatgtac cgaacttgcg
     3361 ctcccagatc gtccactcct acgacgtggg cgaggccttc aggctcgcca tcgtcaacga
     3421 cgaggcgcgg ggctcgttca acctggctac cgagccggtc ctggacccgg c
//
LOCUS       DASTUV010000421         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1333123, whole genome shotgun sequence.
ACCESSION   DASTUV010000421 DASTUV010000000
VERSION     DASTUV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333123"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFT03_08490"
     CDS             complement(<1..1178)
                     /locus_tag="VFT03_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU4848222.1"
                     /translation="MKRLRVLMTREEIVPERLAGATAVVVDVFLATTTLLTILENGAR
                     RVFPVASLEEAEEVGAKLDASGVLRGGEQDAARIEGYDHGPFPDEYPPERVADRDVIF
                     VTTNGTRAIADAAPAGRVLLGCLRNAPAVARYLESSGAESVYLICAGSAGRFTIEDFL
                     GAATVLSHMNPGGWRLNDGAWMALDFMKRHRNRELEALKQSRAGRWFFEHDRVDAFEF
                     VGETGSSELVPEVKDGQLVSTLPTEGRRRAEMGGKPLDTIRVREGEGFDLEAVERYLR
                     EHIDGLPEGELKVRQFPSGASNLTYLLEVGDWEGVLRRPPFGPVPPKAHDMGRESGVL
                     MRLHEAYPLAPKPYFFCDDESVIGAPFYVMERRRGVVVDDEFPEGVVPTRELCQGISR
                     A"
     gene            1328..1762
                     /locus_tag="VFT03_08495"
     CDS             1328..1762
                     /locus_tag="VFT03_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848223.1"
                     /translation="MSDLENLRREEPEEAGTAAYVSVALGIGLLVTTVFVIITAGALA
                     AGFPFVLGAAACLGWGTSRFARQRHALPESGGERALLSALRENGGGITPAEAAMETSL
                     TVKEADRMLSQLASEGHLLVESREGSLFYALPRRSSPQLEGR"
     gene            complement(1845..>2111)
                     /locus_tag="VFT03_08500"
     CDS             complement(1845..>2111)
                     /locus_tag="VFT03_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU4848224.1"
                     /translation="AYRIGLVNRVVPDEMLMEETAAFAKRLAAMPTRALGSMKQALYA
                     SFESDLETALEREAVGQALCGYTRDHAEGLAAFFEKREPDFTGR"
BASE COUNT          342 a          709 c          669 g          391 t
ORIGIN      
        1 gcgcgggaga tcccctggca cagctcgcgg gtgggcacga ccccctcggg gaactcgtcg
       61 tccaccacca cgcctctgcg gcgctccatt acgtagaagg gcgcccctat taccgactcg
      121 tcatcgcaga agaagtaggg cttcggggcg agtgggtaag cctcgtgcag cctcatgagc
      181 acgccggact cccggcccat gtcgtgggct ttgggaggta cgggtccgaa cggagggcgt
      241 ctgaggacgc cctcccagtc ccctacctcg agcaggtagg tgaggttaga agcccctgag
      301 gggaactggc ggaccttcag ctcgccttcg ggaaggccat ctatgtgctc ccggaggtac
      361 cgctcgaccg cctcgaggtc gaacccctcc ccttcgcgca ctcgtatcgt atccaaaggt
      421 ttgcctccca tctcagcacg ccgcctccct tcggtcggca gcgtgctaac aagctggccg
      481 tccttcactt ccgggacgag ttcgctcgag ccggtctcgc cgacgaactc gaaggcgtcc
      541 acccggtcgt gctcgaagaa ccaccggccc gcccggctct gtttgagggc ttccaactcc
      601 ctgtttctat gacgtttcat gaagtcgagc gccatccacg cgccgtcgtt cagccgccat
      661 cctccggggt tcatgtgaga gagaacggtg gccgcgccga ggaagtcttc gatggtgaag
      721 cgtccggcag agccggcgca gatcaggtag accgactcgg cacccgagct ttcgagatag
      781 cgggctacgg cgggtgcgtt gcggaggcag ccgaggagaa ctctccccgc tggggcggcg
      841 tcggctatcg cgcgggtgcc gttggtggtc acgaagatca cgtccctgtc cgccaccctc
      901 tcgggcgggt actcgtcggg gaaggggccg tggtcgtagc cttctatcct cgcagcgtcc
      961 tgctcgccgc cgcgcaggac gccagaggcg tcgagcttcg ccccgacctc ttcggcctcc
     1021 tccaggctgg cgacggggaa gacgcggcgg gcgccgttct cgaggatggt caggagggtg
     1081 gtagtcgcca ggaacacgtc cactacgacg gctgtcgcgc ccgccagcct ctccggcacg
     1141 atctcctccc gggtcatcag cacccttagc cgcttcaagg ccgatcagcc tgtcgcatct
     1201 ctcgactcac gaaatcgtat tctcgcactt ttttcgggtc ctaacggagg gcgacccgct
     1261 ttgcagcccc tgacgccgcg caatagaatt ggccgggaat ccagatggga aggagagccg
     1321 ggagatagtg agcgatctgg agaatctcag acgggaagaa ccggaggaag cgggcaccgc
     1381 cgcctacgta tccgtggcgc tcgggatcgg gctcctcgtc accactgtgt tcgtgataat
     1441 caccgcgggt gcactcgcgg ccggcttccc attcgtgctg ggcgccgccg cctgtttggg
     1501 ctggggaacc tcccggttcg cgcggcagcg acacgctctt cctgagtccg gcggggagcg
     1561 cgcgttgctc tcggccctcc gggagaacgg cggcggcatc acacccgccg aagccgcgat
     1621 ggagacttcg cttacggtca aagaggcgga ccgtatgctc tcgcagctgg cctccgaggg
     1681 tcatctcctg gtcgagagcc gggaggggtc gctgttctac gcgctccctc gcaggtccag
     1741 cccgcaactc gaaggccggt aagggcagat ctccacgcta gcaagtccct ctcgatagcg
     1801 tcacacatct ctgtacgttt ctgccagaga aggttttacc gttcttagcg tccggtgaag
     1861 tcaggttcgc gcttctcgaa gaaggccgcg aggccctccg cgtggtcccg tgtataaccg
     1921 cagagcgcct ggcctacggc ctcccgctcg agcgcggtct cgaggtcgct ctcgaagctc
     1981 gcataaaggg cctgcttcat gctccccaag gcccttgtcg gcatcgccgc caatcgcttc
     2041 gcgaaggctg ctgtctcttc catgagcatc tcgtcgggaa caacccggtt gaccagccct
     2101 atgcggtacg c
//
LOCUS       DASTUV010000422         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_536520, whole genome shotgun sequence.
ACCESSION   DASTUV010000422 DASTUV010000000
VERSION     DASTUV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536520"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFT03_08505"
     CDS             <1..949
                     /locus_tag="VFT03_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4848225.1"
                     /translation="RTFDITHPDDLEASIARTRALLEGTVERDLLEKRYIRADGDIVW
                     ALSSVSLVRDSRGDPAYFVSQYQNITERKKAEEDMKEANRRLEELAVLKADFTAMVAH
                     ELDTPLAVIRGYADMLATGELDIDERARALDRVQAETDLLEALVSDVRSAAAVERGDF
                     ASELQQVALDGLLRDAAAFVETLQGDHRLLTQGATDGWVLADPHRIRQVLRNLLSNAA
                     KYSPDGTPIELRTAPGATPGRVRIEVADRGSGIHPDDAARIFEKYGRGRDRDGRKVAG
                     TGLGLYLSKRILQAHGSELTLDTEPGGGSVFGFELRAGR"
     gene            946..1593
                     /locus_tag="VFT03_08510"
     CDS             946..1593
                     /locus_tag="VFT03_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848226.1"
                     /translation="MNFVLLVDDHATFREPLAFMFNREPEFEVIAEAGTLAEAKEMLE
                     GIDLAVVDLDLPDGDGTQLIGDLRAANPHGAVLVLTASADREAYARAVEAGAAGVLHK
                     SVRIKEVVDAARRLVSGEPLLSTNEVIELLQLAGRRREQNYEARRAIESLTPRELEVL
                     EALAAGLSDKEISERLHVGIGTVRNHVVSIFNKIGVHSRLQALVFGVRHGVVEIR"
     gene            complement(1658..3469)
                     /locus_tag="VFT03_08515"
     CDS             complement(1658..3469)
                     /locus_tag="VFT03_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEU4848227.1"
                     /translation="MKSGLDALERLRGSVHEGLGRTAVAYVVLGAALVLTGLAFLYVR
                     HNVEERERERFEEITVSTERAVDRRMQTYIDAMLDGRGLFAASESVTREEWEDYVAGS
                     ELERRYPGIQAIAYAERVPLEGREAHERRVRAQGFPSYALRPAGERYEYFPLVYVAPS
                     KPDNRSLFGYDFYSGRVNRDAMEQARDTGLPHASGKVELEGTGMTGFLVYTPIYRKGA
                     PQATTDERREALQGFIVSVFRTDDLLEGIFGARKADTQVDLEVFDGAEFTRDGLLHDD
                     DGVLHATDPSYSAHFSEVDTMEVAGRIWSLYFSTLPSFEPGWRSNLPLFVLLSGLAIS
                     LMLFGVTWMLAHSRIQAGHVGLQLEAANRQLEKTNRDLEETNRELEGANSELEAFSYS
                     VSHDLRSPLRSIEGFAQILLEDHAQGLDEEARGYLRRVRAASRRMALLIDDLLDLSRV
                     TRSSLTRQMVDLSALAREIAAEIEKSQPDRRVRFVIADGLAVRGDPRLLRVLLENLLV
                     NAFKFTQKEREATIEFGAVTRDGGVAYYVRDNGVGFDEAYATKLFGAFQRLHGSEDFE
                     GTGIGLATVQHIVQRHGGKAWAEGAVGKGATFFFTLR"
     gene            complement(3475..3918)
                     /locus_tag="VFT03_08520"
     CDS             complement(3475..3918)
                     /locus_tag="VFT03_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4848228.1"
                     /translation="MRDKIILLVEDNPDDELLTMRALRKNNVLNKVVVARDGVEALDY
                     LFGTGRYSGRDTAVMPQLILLDLKLPKIDGLEVLKRLRADERTRLLPVVILTSSREEQ
                     DMLDGYGFGANSYVRKPVNFEQFVQAVEQLKLYWLILNEAPPAAV"
     gene            complement(3932..4366)
                     /locus_tag="VFT03_08525"
     CDS             complement(3932..4366)
                     /locus_tag="VFT03_08525"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEU4848229.1"
                     /translation="MQEERNVGGPTEATEQMSAQDEKTWSVVAHLSVLAALVGLMPFG
                     ALLVWLLYKDRSPRVRFHAAQAFWYQVAWIVIFLVYALVTVVLSVVTIGMAAIVLVPL
                     AFLLALVPLAHGCYAAYKVNQGEEYRYPFIADRVDGGASRVF"
BASE COUNT          809 a         1518 c         1543 g          801 t
ORIGIN      
        1 gaggacattc gacatcaccc accctgacga cctcgaggcc agtatcgccc gcacgcgcgc
       61 attgctggaa ggcaccgtag aaagggacct cctggagaag cgctacatac gtgccgacgg
      121 ggacatcgtg tgggctctat cgagcgtctc gctggtgcga gactcacggg gagatcctgc
      181 ctacttcgta agccagtacc agaacataac cgagcgcaag aaggcggagg aggatatgaa
      241 ggaggccaat cggcggctcg aggagctggc ggtgctcaag gccgacttca cggcgatggt
      301 ggcccacgag ctagacacgc cgcttgcggt gattcgcggc tacgctgaca tgctggcgac
      361 gggcgagctg gacatcgacg agcgcgcgcg ggcgctcgac agggtccagg ccgagaccga
      421 cctgctcgag gcactagtct cagacgtgag gtcggccgcg gccgtcgaaa ggggggactt
      481 cgcaagcgag ctccagcagg tggctctcga cgggctcttg agagacgcgg cggccttcgt
      541 ggagacgctg caaggagacc accggctcct cacgcagggc gccacggacg ggtgggtgct
      601 cgcggacccg cacaggatca gacaggtgct caggaacctg ctgtccaacg ccgccaagta
      661 ctcgcccgac ggcacgccga tcgagctccg cacggcgcct ggcgcaacgc cagggcgcgt
      721 gcgcatcgag gtggcggacc gtgggtcggg catacacccc gacgacgcgg cccgcatctt
      781 cgagaagtac ggccggggac gggataggga cggcaggaag gttgccggta cggggctggg
      841 tctctactta tccaagcgca tcttgcaggc ccacgggagc gagcttacgc tggataccga
      901 gccgggcggg ggctcggtat tcggcttcga gttgagggct ggccggtgaa cttcgtcctg
      961 ctcgtcgacg accatgcgac cttccgcgag ccgctcgcct tcatgttcaa ccgggaaccg
     1021 gagttcgagg tcatagctga ggccgggact ctcgccgagg cgaaggagat gctcgagggt
     1081 atcgacctcg cggtcgttga cctcgacctc cctgacgggg acgggaccca gctgataggc
     1141 gacctgcgcg ccgccaaccc gcacggcgcg gtgctggtct tgacagcgtc cgccgaccgc
     1201 gaggcatacg cgagggcggt cgaggcgggc gcggccggcg tgctccacaa gtcggtgcgc
     1261 atcaaggagg tcgtcgacgc ggcacggcgg ctcgtctcgg gcgagccgtt gctctcgacc
     1321 aacgaggtga tcgagctgct ccagctcgcc ggccgccgca gggagcagaa ctacgaggcc
     1381 cggcgcgcca tcgagagcct cacgcctcgc gagctggaag tgctcgaggc gctcgccgcc
     1441 ggcctgagcg acaaggagat ctcggagcgt ctccacgtcg gcatcggcac ggtgcgcaac
     1501 cacgtcgtga gcatcttcaa caagatcggg gtgcactcgc ggctgcaggc gctcgtcttc
     1561 ggcgtgcgcc acggcgtcgt cgagatccgc tgagcagcac gctacgccct tttagggctt
     1621 cgcttgtcag cacggcgcct gctcccaata cccttcatca cctgagcgtg aagaagaagg
     1681 tggctccctt tccgaccgca ccctcggccc acgccttccc cccgtgacgc tgcacgatgt
     1741 gctgtacagt ggccagccct atgcccgtcc cctcgaagtc ctcggagccg tgcagcctct
     1801 ggaacgcgcc gaagagcttg gtcgcgtagg cctcgtcgaa gccgaccccg ttgtcgcgca
     1861 cgtagtaggc gacacctccg tctcgggtga cggcgccgaa ctctatggtg gcctctctct
     1921 ccttctgggt gaacttgaag gcgttgacga gcaggttctc cagcagtacc cgcaagaggc
     1981 gggggtcgcc gcggacggcg aggccgtcgg cgatcacgaa cctcaccctc cggtcgggct
     2041 gggacttctc gatctccgcg gctatctcac gggccagcgc actcaggtcc accatctgcc
     2101 tggtgaggga gctgcgcgtc accctggaga ggtcgagcag gtcgtctatg aggagggcca
     2161 tgcgcctgct cgcggcccgc accctccgaa ggtagccccg ggcctcttcg tcgagcccct
     2221 gagcgtggtc ttcgaggagg atctgggcga agccctcgat gctccgcaag ggcgacctca
     2281 ggtcgtggga gaccgaatag gagaaggcct cgagctcact gttggccccc tcgagctccc
     2341 tgttggtctc ctccaggtcg cggttggttt tctcgagctg tctgttggcg gcctcgagct
     2401 ggaggccgac gtgtcctgcc tggatgcggc tgtgcgcgag catccaggtc acgccgaaga
     2461 gcatcaggct gatcgcgagc ccgctgagca gcacgaagag ggggaggttg ctccgccagc
     2521 cgggctcgaa gctcgggagc gtgctgaagt agaggctcca gatcctgccg gccacctcca
     2581 tggtgtcgac ctcggagaag tgcgcggagt aggaggggtc cgtggcgtgc aggaccccat
     2641 cgtcgtcgtg gagcaagcca tccctggtga actccgcccc gtcgaacacc tcgaggtcga
     2701 cctgcgtgtc tgccttccgc gcgccgaaga tcccctcgag caggtcgtcg gtgcggaaca
     2761 cgctgacgat gaacccctgc aacgcttcgc ggcgctcgtc cgtggttgcc tgcggcgcgc
     2821 ctttgcggta gatgggggtg tagacgagga agcctgtcat gccagtacct tcgagctcga
     2881 ccttgcccga ggcgtggggt agacccgtgt cgcgggcctg ctccatggcg tcacgattaa
     2941 cccggccaga gtagaagtcg tagccgaata gcgaccggtt atccggcttc gagggcgcta
     3001 cgtagacgag cgggaagtat tcgtagcgct ctccggcggg tcgcagggca taggaaggga
     3061 agccctgcgc gcggacgcgc cgctcgtggg cctccctgcc ctcgaggggc acgcgctcgg
     3121 cgtaggcgat cgcctgtatg ccggggtagc ggcgttccag ttcgctgccc gccacatagt
     3181 cctcccactc ctcgcgcgtg acggactcgc tcgcggcgaa gaggccccgc ccgtcgagca
     3241 tagcgtctat gtaggtctgc atgcggcggt ccacagcccg ctcggtggag acagttatct
     3301 cctcgaaccg ctcgcgctcg cgctcctcga cgttgtgcct gacgtacaag aaggcgaggc
     3361 cggtgagcac aagcgccgcg cccagcacca cgtaggccac ggcggtccga ccgagcccct
     3421 cgtgcacgga gccgcgcaaa cgctccagcg catcgaggcc agacttcacg aggcttacac
     3481 cgccgcgggc ggcgcctcgt tcaggatcaa ccagtagagc ttgagctgct cgacggcctg
     3541 gacgaactgc tcgaagttga ccggcttgcg gacatagctg ttggcgccga agccgtagcc
     3601 gtcgagcatg tcctgctcct ccctcgagga ggtcaggatg accacgggca agagccgcgt
     3661 cctttcgtcg gccctcaggc gcttcaagac ctccaggccg tctatcttgg gcagcttgag
     3721 atccagcagg atcagctgcg gcatgacggc tgtgtcccta ccggagtacc tgccggttcc
     3781 gaacaggtag tccagcgcct cgacgccgtc acgggccacc acaaccttgt tgaggacgtt
     3841 gttcttcctg agcgcccgca tcgtcaggag ctcatcgtcg gggttgtcct cgaccaggag
     3901 tatgatcttg tccctcatac cgccgcgtct ctcagaaaac tctgctggcg ccgccgtcta
     3961 ccctgtcggc tataaaggga tagcggtact cctcaccctg gttcaccttg taggccgcgt
     4021 agcatccgtg cgccaggggc accagggcga gcaggaacgc gagcggtacg agcactatgg
     4081 cagccatgcc gatcgttacc acggacagga ctaccgtcac cagcgcgtag acgaggaata
     4141 taacgatcca ggcgacctgg taccagaacg cctgggcggc gtggaagcgt acccgtgggg
     4201 agcggtcctt gtatagcagc cagaccagga gcgccccgaa cggcatcaac cccacgagcg
     4261 cggcgagcac actcaggtgc gcgacgacgg accacgtctt ctcatcctgc gcgctcatct
     4321 gttctgtcgc ctccgttggg cctccgacgt tgcgttcttc ttgcatctct ctcctttcgt
     4381 cccggctctc atgcccgcag catcgggcat tgataatctt agccccgccc gggcctggcg
     4441 agcatcactc atttgagtga tttttcagta cagcaagccc gcaaaagaga aacgggccgg
     4501 cttccaggag gggggagatg gggcgccggc ccgctaatgt gagatcgagc cggtccacca
     4561 aggagggggg aaggtatgtg gcaccgcctc gttcttcctg ctgcgattat gcaccctagt
     4621 aacccaacgc acatcactca aatgagtgat tttttgaaag tttttctcag g
//
LOCUS       DASTUV010000423         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_726327, whole genome shotgun sequence.
ACCESSION   DASTUV010000423 DASTUV010000000
VERSION     DASTUV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726327"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..713
                     /locus_tag="VFT03_08530"
     CDS             150..713
                     /locus_tag="VFT03_08530"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEU4848230.1"
                     /translation="MAEPVKPHKLPTVKEYLALETDSTVRHEYVAGMIYAHAGGTSRH
                     NAIAVNVLAALWNAARGGECRVYGSDMMLRATDDVFYYPDMTVVCGDDEAGDEAVYQH
                     APCLIVEVTSPSTESIDRREKAFTYRKIPSLRAYLVVDQNRRWVERHWRDEAGEWRQG
                     GVADEGFVPIPCPETRLSLDEIYEGLG"
     gene            complement(726..1562)
                     /locus_tag="VFT03_08535"
     CDS             complement(726..1562)
                     /locus_tag="VFT03_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4848231.1"
                     /translation="MHNLPKPLWLSELVAVWGYVQRNYFLTKRYLMWEIVWLVYVTVN
                     AMAITYIGAGVEAVGGSVDTRFLMTYLLVGALLWNYLSMLFDVLSETVSWERWEGTIE
                     YTFMSPSSRVTHLLGMGFYAVIYGILQTVVTLGICYLFFDLDLSQANYWGALLILAVC
                     SVSLVGFGVVAAVLPLLSPEKGQQVSYIVSSVLLLVSGVYYEVDVLPGWMQAIATVSP
                     VTYALQGARAALLEGAGAAELWSSIWPLLIMGVVFVPLGLFVFHLGESYAKRTGKLKR
                     SG"
     gene            complement(1562..2350)
                     /locus_tag="VFT03_08540"
     CDS             complement(1562..2350)
                     /locus_tag="VFT03_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4848232.1"
                     /translation="MNALELRNVRKSFNKRRGEVVHALVSVSLAVGRGEVVGILGPNG
                     SGKSTLVRVVSTLVVPDAGSVEVFGVDAVRHPKKVQRSMNRVSVEASFFKKMSAMENL
                     LYGAKLYGVTTGEARPRIKEIFENIGFAHKRALEPMEHLSRGMQQKVALARALLTSPI
                     LMLLDEPTTGLDPRSKKDVQDFIRRIREAHDSSILLCTHDMDEAEELCDRVGIMVDGR
                     VLALDKPEELKRRYASNGRVPTLEETFMAATGYSVEEASAEGED"
     gene            complement(2397..>2598)
                     /locus_tag="VFT03_08545"
     CDS             complement(2397..>2598)
                     /locus_tag="VFT03_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848233.1"
                     /translation="YMMEDRGLRQQDLAQRVAERESYARSKHKSSLRARIARRLFELA
                     VAAEREETWRLIWERLEARGRL"
BASE COUNT          464 a          865 c          807 g          462 t
ORIGIN      
        1 tagtcttcgt ccccctgggg ctcttcgtct tccacctcgg cgagagctac gccaagcgca
       61 ccgggacgct caagaggagc gggtaatctg ccttgcaccc ggtataactt ggcgaccgcc
      121 atgcacggcc gtcaagttat actggtttca tggcagagcc cgtcaagccg cacaaactac
      181 cgaccgtcaa ggagtacctg gcgctcgaaa cggatagcac cgtccgccac gagtacgtgg
      241 ccggcatgat ctacgcgcac gcaggcggca ccagcaggca caacgcgatc gccgtcaacg
      301 ttttggccgc gctctggaac gcggcccgcg gcggcgagtg tcgtgtctac ggcagcgaca
      361 tgatgctacg agccaccgac gacgtctttt actaccccga catgacggtg gtctgtggcg
      421 atgatgaagc cggcgacgaa gcggtctatc agcacgcccc ctgcctgatc gtcgaggtca
      481 cgtcgccgag caccgagtca atagatcggc gcgagaaggc gttcacctac cgcaagatcc
      541 cgagcctgcg agcctacctt gtcgtggacc agaaccgccg ctgggtcgag cgccactggc
      601 gcgacgaggc cggcgagtgg cgtcagggcg gggtagccga cgaaggtttc gtcccgatcc
      661 cctgccccga aacgaggctc tccctcgacg agatctacga gggtttggga taggcttcgg
      721 atcttctatc cgctcctctt gagcttgcct gtgcgcttgg cgtagctctc gccgaggtgg
      781 aagacgaaga gaccgagtgg tacgaagacc acgcccatga tcaggagcgg ccagatgctg
      841 ctccagagct ccgcggcgcc agccccttcg agcagggcag ccctcgcacc ctgtagcgcg
      901 taggtgacgg gggagacggt agctatggcc tgcatccagc ccgggagcac gtccacctcg
      961 tagtagacgc cggagacgag cagcagcacg gacgagacta tgtagctgac ctgctggccc
     1021 ttttcggggg agagcagcgg cagcacggcg gcgacgacgc cgaagccgac gagcgagacg
     1081 ctgcacaccg cgaggatcag gagggcgccc cagtagttcg cctggctcag gtccaggtcg
     1141 aagaacaggt agcagatgcc aagtgtgacg accgtctgca ggatgccgta gatgaccgcg
     1201 tagaagccca tcccgaggag gtgggtcacg cggctcgagg ggctcatgaa cgtgtactcg
     1261 atggtcccct cccaccgctc ccagctcacc gtctcggaga gcacgtcgaa gagcatcgag
     1321 aggtagttcc agaggagggc gccaacgagc aggtaggtca tgaggaacct ggtgtccacg
     1381 gatccgccca cggcctcgac gcccgcgccg atgtaggtga tcgccatggc gttcaccgtg
     1441 acgtagacga gccagacgat ctcccacatc aggtagcgct tggtcaggaa gtaattgcgc
     1501 tgtacgtagc cccataccgc gaccagctcg gagagccata aaggtttggg caggttgtgc
     1561 atcagtcttc tccttccgcc gacgcctcct ctacggagta gccggtcgcg gccatgaagg
     1621 tctcctccag ggtagggaca cggccgttgc tcgcgtagcg tcgcttgagc tcttccggct
     1681 tatcgagagc caggactctg ccatcgacca taatgccgac gcggtcgcag agctcctcgg
     1741 cctcgtccat atcgtgggtg cagagcagga tcgaggagtc gtgcgcctcc ctgatgcgcc
     1801 ttatgaagtc ctgcacgtcc ttcttgctcc tggggtcgag gcccgtcgtc ggctcgtcga
     1861 ggagcatcag gatggggctc gtcaggaggg cccgcgcgag cgccaccttc tgctgcatgc
     1921 cccggctcag gtgctccatc ggctccagcg cccgcttgtg ggcgaagcct atgttctcga
     1981 agatctcctt tatcctcggg cgtgcctcgc cggtcgtcac accgtagagc ttcgcgccgt
     2041 agagcaggtt ctccatagcc gacatcttct tgaagaagct cgcctcgacg ctgaccctgt
     2101 tcatgctccg ctgcaccttc ttcgggtgcc tcacggcgtc aacgccgaag acctccaccg
     2161 agccggcgtc gggtacgacg agcgtcgaga ccacgcgcac gagcgtgctc ttcccgctgc
     2221 cgttcggccc gaggatgccg accacctcgc cccgcccgac cgcgagcgag accgagacga
     2281 gggcgtgcac gacctcgccc cgccgtttgt tgaagctctt ccgtacgttg cgtagctcta
     2341 aagcgttcac gagcagtttc ccttcgtttt ctacttcccc cgcgaggggg ccggctctac
     2401 aaccggcccc tcgcttccag cctctcccag atcaggcgcc aggtctcctc ccgctcggcg
     2461 gctaccgcca gctcgaagag ccggcgcgct atccgggccc gcaaggatga cttgtgtttc
     2521 gagcgcgcgt agctctcgcg ctcggccacc ctctgggcga ggtcctgctg acgcagaccc
     2581 cgatcctcca tcatgtag
//
LOCUS       DASTUV010000424         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1368566, whole genome shotgun sequence.
ACCESSION   DASTUV010000424 DASTUV010000000
VERSION     DASTUV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368566"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..846
                     /locus_tag="VFT03_08550"
     CDS             112..846
                     /locus_tag="VFT03_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4848234.1"
                     /translation="MAAQHERRSVESGEEHPGYVFEQFRVARGCLGYVLADRKTRQAA
                     IVDPELEMVESMLDFVFEHGLKPFYVVDTHTHADHFSAARELKAKTVARIVMHEEAPS
                     SAVDLRVEDGDRLWLGGDLPLKFLHTPGHAKDLVSVLVPGRVLTADALLIGSCGRTDL
                     PNGNATRQYHTLYYVFRSLPDGLLVYPGHDYEGRSHSTLGDEKKSNPKMNFPSEEEFV
                     EYMEKENPDTLTPVHQLADALKANMA"
     gene            961..2403
                     /locus_tag="VFT03_08555"
     CDS             961..2403
                     /locus_tag="VFT03_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU4848235.1"
                     /translation="MKVAGAGAAGITLLGTAGCSSLARLPEEYLPRGGSRMNVVVVIV
                     DSLRKDHVGAYGNSWIKTPSLDALAKESLRFTRAYPEAIPSIPARRAIHTGIRSFPFR
                     GWEKWCEDDVGLRGWQPIPRDQTTLAEIMADQGYQNLFVTDTLHQFRPFYDMHRGFDV
                     FHFIRGQERDLFRPRTPASSKKIDEVLIGGPSASHAEDIMLQYLANTMGREREEDWFA
                     PQVFMKGMEFLEAARGEQPFFLVVDNYDPHEPWDPPEKYTSLYDEGYGGQEPWTSSSG
                     PSDWLTERQLQRMHGLYSGEVTMMDTWLGNFLDKMAELNLFENTLLILLSDHGHAFGE
                     HGFAGKVPAALYPELTDITYMIRHPSGKGAGQTSDYFASTHDVAPTVLGALGIEAPSA
                     MQGQDLTVLLEGGDPEQPREHFTAGYHDHVWARDQDYAMFAASNGSEPHLFDLREDPE
                     MKKNIAGSNADIVKRMYNDYLVKDAGGPLP"
BASE COUNT          510 a          829 c          824 g          409 t
ORIGIN      
        1 ccacattttt agcagatatc caggccggcg cccgtccctg ttgccccgcc tgtggcgcga
       61 cggtggcaaa atacctccaa gaggtgccgg acggcgggaa gaggaggtct cgtggcggcg
      121 caacacgaac ggcggtcggt agagtccggc gaggagcacc ctgggtacgt cttcgagcag
      181 ttccgggtgg cgcgcggatg cctcggctac gttctcgccg accggaagac ccgccaggcc
      241 gcgatcgttg accccgagct ggagatggtc gagtcgatgc tcgacttcgt cttcgagcac
      301 ggtctgaagc ccttctacgt agtggacacc cacacccacg cggaccactt ctccgcggcg
      361 cgcgagctca aggcgaagac ggtggccagg atcgtgatgc acgaggaggc gccgtcgagc
      421 gctgtggacc ttcgggtgga ggacggggac cggttgtggc tgggcggaga tctaccgctg
      481 aagttcctgc acacgccggg gcacgcgaag gacctggtct cggtgttggt gccgggaagg
      541 gtcctcaccg ccgacgccct gctcataggc tcgtgcggac ggaccgacct gccgaacggc
      601 aacgccacca ggcagtacca cacgctctac tacgtcttca ggagcctgcc cgacgggttg
      661 ctcgtctacc ccggccacga ctacgagggc cggagccact cgactctggg cgatgagaag
      721 aagagcaacc cgaagatgaa cttcccctcc gaggaggagt tcgtcgagta catggagaaa
      781 gagaaccccg acacgctgac cccggtccac cagctcgccg atgcgctcaa agccaacatg
      841 gcctgatctc cggtcgaggg agctcatatt ctctacaata tgcggctgcc cgagagacgt
      901 ggcgcaagca tcataggcga gagggatggt ttggaacgta ctctaaccag aagagatttc
      961 ttgaaggtcg cgggggccgg ggcggccggt atcaccctgc tcgggaccgc cggttgtagc
     1021 agcctcgccc ggctgcccga agaatacctt ccgaggggag ggtccaggat gaacgtcgtc
     1081 gtggtgatcg tagacagctt gaggaaggac cacgtcggag cctacggtaa tagttggata
     1141 aagacgccga gcctggacgc tttggcgaag gagagcctgc gcttcacccg ggcttacccg
     1201 gaggcgatac cctccatccc ggcgcggcgc gcgatccaca cgggcatcag gtccttcccc
     1261 ttcaggggat gggagaagtg gtgcgaggac gacgtgggct tgaggggctg gcaacccatc
     1321 cccagggacc agacgacgct ggcggagatc atggccgacc agggctacca gaacctattc
     1381 gtcacagaca ccctgcacca gttcaggccc ttctacgaca tgcaccgcgg cttcgacgtc
     1441 ttccacttca tcaggggcca ggagagggat ctcttcaggc cgcgcacgcc ggcctcgagc
     1501 aagaagatag acgaggtcct tatcgggggt ccctccgcct cgcacgccga ggacatcatg
     1561 ctccagtacc tggcaaacac catgggccgc gagcgcgagg aggactggtt cgccccgcag
     1621 gtgttcatga aggggatgga gttcctcgag gctgcgaggg gcgagcagcc cttcttcctg
     1681 gtagtggaca actacgaccc gcacgagccc tgggacccgc ccgagaagta cacctccctc
     1741 tacgacgagg gatacggcgg ccaggaaccc tggacctcga gcagcgggcc ctccgactgg
     1801 ctcaccgagc gtcagctaca gaggatgcac ggtctctact ccggggaagt cacgatgatg
     1861 gacacctggc ttggcaactt cctcgacaag atggccgagc tgaacctctt tgagaacacg
     1921 ctcctcatcc tcctctccga tcacggccac gccttcggcg agcacggttt cgccggcaag
     1981 gtccccgcgg cgctctaccc cgaactcacg gacatcacct acatgatccg gcacccgagc
     2041 ggcaagggcg caggccagac tagcgactac ttcgcctcga cccacgacgt cgccccgacc
     2101 gtgctcggcg ccctggggat cgaagctccc tccgcgatgc agggccagga cctcacggtg
     2161 ctgctcgagg gcggcgaccc cgagcagccg cgcgaacact tcacggccgg ctaccacgac
     2221 cacgtctggg cccgcgacca ggactacgcc atgttcgccg ccagcaatgg ctccgaacct
     2281 catctcttcg acctgcggga ggaccccgag atgaagaaga acattgcagg gtccaacgcc
     2341 gacatcgtaa agcggatgta caacgattac ctggtgaagg acgcgggcgg accgttaccg
     2401 tagcatttca gcacgcttcg gcccttcggg cctccgcttg tcagcatttc agcttctctc
     2461 tgagctgagc gaagctgaga agccgcctgg cgaagccagg tggcgtgctg agagcgaggc
     2521 ccgcaagggc cgaagcgtgc tgaaagcgaa ggcgaggcct gagcgtgctg ac
//
LOCUS       DASTUV010000425         6178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_827876, whole genome shotgun sequence.
ACCESSION   DASTUV010000425 DASTUV010000000
VERSION     DASTUV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827876"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFT03_08560"
     CDS             <1..285
                     /locus_tag="VFT03_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4848236.1"
                     /translation="YGESPYAYLMTRRIERAMALLRRGDLSVTEVCFAVGCSSLGTFS
                     TRFTELVGVPPSVYRREAARATEGMPACVAKQVTKPIRNREAPVTEPKLA"
     gene            291..701
                     /locus_tag="VFT03_08565"
     CDS             291..701
                     /locus_tag="VFT03_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4848237.1"
                     /translation="MDITIHTSFLPHDDPEASLAFYRDTLGFEVRNDVGYGGMRWITV
                     GPASQSGTSIVLHPPAADPGITDDERRTIAEMMAKDTYAAIILATKDLDGTFERLQAS
                     GAEVVQEPTEQPYGLRDCAFRDPAGNLIRIQELR"
     gene            1059..2249
                     /locus_tag="VFT03_08570"
     CDS             1059..2249
                     /locus_tag="VFT03_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU4848238.1"
                     /translation="MGKPGLLKQLNASVLLDAIRDEGPLSRPDLARLTGLSLPTVNAR
                     VRRLVQAGYVREAGRTESRGGRRARLLEFNGKFGYVAGIDVGGHQVSAAVADLSGEVV
                     TFERRPLGERVSGERILKGAWEVLCRELEERGIGVGDLMAVGVSTPGIVDPESGSVTC
                     VPNIPGWSELRPDEQFGELIGKTVVVENNVNAAMEGEHWRGAARGARDAIFVAVGTGV
                     GAGILIGGRLFRGWKGAAGEIGLQREFHDDEPLDAMFGPFERRASGIGIAERYRELAG
                     VKRATARTIFEAAANGDELARRVVEEATSLLAGGLVNMCAVLAPELVVLGGGVARAGE
                     ALVGPIRERLERSLPAPPRCVLSELGDQASVVGAVRLALGAAYREEFSFGLKRDYAGR
                     SVRA"
     gene            2246..3535
                     /locus_tag="VFT03_08575"
     CDS             2246..3535
                     /locus_tag="VFT03_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4848239.1"
                     /translation="MSTAEGTEGPRRLSRREFLGLGAAGTGLLLLGGCGGGESGGSGT
                     LNALFMKQAAYSDTDVKSMTEQFQKQNPGIKVNLTFVAYEALHDKIVAAAPAGTYDTV
                     LVDVIWPAEFASKKMIVDITDRFPESERSKIFAGALKTTEYDGRYYGVPWILDTKYFF
                     YNKKMLGEAGVSPPDTWDGVVEAARAIKSKGVVEYPLIWSWAQAEALICDYTTLLGAY
                     GGQFFDESGEPAFNSGGGLQALEFMRMTLDEKLSNPASTESLEEDVRRIISQGEAAMA
                     LNWTYMFALANDPNESQVAGQIDIAHTPEGPAGALGVNGSMGIAIANGSQNQDAAWKY
                     IQFMTSQKIQNQYAKLSLPIWKSSYEQEEVIQALPQVVPIAKVQLNDMILRPVVTNYN
                     DVSHTLQVEIQQALTGEKSPKQALDDAASAVAETQQS"
     gene            3532..4473
                     /locus_tag="VFT03_08580"
     CDS             3532..4473
                     /locus_tag="VFT03_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4848240.1"
                     /translation="MSHGRAEKLKKLPRAVFPSGGAFRQGTTALILLLPSLVVVFGIV
                     LYPLLRTLYISLFDVNSAFPGSYPFVGLGNYMEALGRTEFWAAILRTAYFTLSTTLLE
                     VVLGILLGLLLNAEFRGRWLLRSIVILPWALPTVVNGAMWRWIFNPAYGALNALLTQL
                     HIIPEYRSWLGTPWMALNMVVVADVWKMTPLAAFFILAALQTIPRELYEAAWVDGAGR
                     LRAFFSITLPLLVPTIVIILVLRTMEAFKVFDIIYVMTRGGPADGTQTIAYYTYVEAF
                     SNQLFGYGAALAYVIALFILFFAIIYMRLLRRGEVEY"
     gene            4477..5325
                     /locus_tag="VFT03_08585"
     CDS             4477..5325
                     /locus_tag="VFT03_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4848241.1"
                     /translation="MRNSTGFKVFIYAAAVFVSLIILAPFAWLIISSVAAPADLLTKP
                     LRWIPPHASLSRYSAIFFGANSETVVTFRAALLNSTIVATSSVLISLGVGIFGAYAFA
                     RLQFRFQRGILLVFLSTYMLPPIAILISLYLTMAQLHLLDTRLGLILVYCSFITPFVL
                     WIMSGYFRSIPRDLEDAARIDGCSRIGALFRIVLPLARPGILATALFGFLLAWDEFLY
                     ALVFTSSLSSKTIPVAIAEFTGRHAVDFGMMATGGVLASLPPLLIAFVFQRYIVGGLA
                     SGAVKG"
     gene            5328..>6178
                     /locus_tag="VFT03_08590"
     CDS             5328..>6178
                     /locus_tag="VFT03_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848242.1"
                     /translation="MGFLEDILEQPQNLARSREIFTQALRGADLSAFEADPLVLAGMG
                     SSLFGAIPAACALRGAGRAAFALSATELLEPGGNLLGKAYVGISQSGKSAETVEALSR
                     VSAPRLALTNTGAGPLSEVADVALPIGSAEDTAIAVLTYTATLAATGMLAGALGAPLG
                     FDWDRLPDLVGKVLEDGARAAEDFAERFDGMEFLDFVGRGSSLASAAEGALLAREAVR
                     LPAACLDTLQYLHGPLEVAEPGRGCVLFGSGREVQLAADLASYGASVLLISRASMPST
                     RNLKVFR"
BASE COUNT         1071 a         1919 c         2062 g         1126 t
ORIGIN      
        1 tacggcgagt cgccgtacgc ctacctgatg acccggcgga tcgagcgggc aatggcgctg
       61 ctgcggcgtg gcgacctcag cgtcaccgag gtctgcttcg cggtcggctg ctcgtcgctg
      121 ggcaccttca gcacgcgctt cacggagctg gtcggcgtgc cgccgagcgt gtaccggcgt
      181 gaggcggccc gtgcgacgga ggggatgccg gcgtgcgtgg ccaaacaggt caccaaaccg
      241 atcaggaatc gagaagcgcc ggtcacggag ccgaagctag cgtgaccgcc atggacatca
      301 cgattcacac gagcttcctc ccgcacgacg acccggaggc ctccctggcc ttctaccgcg
      361 acaccctcgg cttcgaggtc cgtaacgacg tcggatacgg cgggatgcgc tggatcacgg
      421 tcggacccgc cagccagtcc ggcacgtcca tcgtcctgca tccgccggcc gccgaccccg
      481 gcatcaccga cgacgagcgc cgcaccatcg ccgagatgat ggccaaggac acctacgccg
      541 ccatcatcct ggccaccaag gacctcgacg gcaccttcga gcggctgcag gccagcggcg
      601 ccgaggtcgt ccaggagccg accgagcagc cctacggcct tcgcgactgc gccttccgcg
      661 accccgcggg caacctgatc cgcatccagg agctgcgctg aaccgtacgg cgatcgcagc
      721 catgagctgc acagacggca ccgtgaaaga gcccgcacac gacgccggcg gtggccgcct
      781 ggtggccacc gtcaccgacc ccgatggcga cgtccccgag ctgcttcaca cccgatgagt
      841 gccacccccc tctcccgcgc tcagcgtcgc atggagtcac gatgagcatg gccacgagta
      901 cggacacgca gtcgcgccga agccctcctc cgccgatgta acgctcctgt tgcgacacca
      961 gagtcctgca caggaggagc gtgttgctca caccatactg ggatagtaaa attaattaat
     1021 taattaagtg gtgcagggga gagactctga gctacacggt ggggaagccg gggctactga
     1081 agcagctcaa cgcgagcgtt cttttggatg ccattcggga cgaagggccg ttatcgaggc
     1141 cggatcttgc gaggctcacg gggttgagcc tgcccacggt gaatgccagg gtgcggcgtc
     1201 tggtgcaggc tgggtatgtg cgtgaggcgg gacgaacgga gtctcgcggg ggacggagag
     1261 ccaggctcct ggagttcaac ggcaagttcg gctacgtagc cgggatagac gtgggtggac
     1321 atcaggtctc ggccgcggtg gcggatctgt cgggcgaagt ggtgactttc gagcggcgtc
     1381 ctcttgggga gcgggtaagc ggtgaacgga tactcaaggg tgcctgggag gttctgtgtc
     1441 gggagctcga ggagcgtggg ataggggttg gggatctcat ggccgtcggg gtctccacgc
     1501 cggggatcgt ggaccccgaa agcgggagcg tgacgtgcgt gcccaacata ccgggatggt
     1561 ccgagctgag gccggacgaa cagttcggtg agttgatcgg gaaaacggtc gtggtggaga
     1621 acaacgtcaa cgccgcgatg gaaggtgagc actggcgcgg cgcggcgcgg ggcgcgcggg
     1681 atgcgatctt cgtagccgtg gggaccggtg ttggggccgg gatcctcatc ggcggaaggc
     1741 tctttcgggg ctggaagggg gctgcggggg agatcgggct gcagcgggag ttccatgacg
     1801 atgagccact ggacgcgatg ttcgggccgt tcgagcgcag ggcttcgggg atcgggatcg
     1861 ccgaacgcta ccgggagctg gcgggggtga agcgggccac agcccgaact atcttcgagg
     1921 ctgcggcgaa cggggacgaa ctcgcgcggc gggtggtcga ggaagctacg tcgttgctcg
     1981 caggagggct ggtgaacatg tgcgccgttc tggcgccgga gctcgtggta ctgggcgggg
     2041 gagtggcgcg tgccggagag gcgctcgtgg ggccgatcag ggaacgtctg gagcgttcgt
     2101 taccggctcc tccgcggtgt gtcctttcgg agctgggaga tcaggcgtcg gtggtggggg
     2161 cggtgcggct tgctctgggc gccgcctaca gagaggagtt ctcttttggt ctcaagagag
     2221 attatgcggg aaggagtgta cgggcatgag cactgctgaa gggaccgagg gacctagaag
     2281 actcagtcgg cgagagttcc tgggcctggg agcggccggg accgggttgc tgttgctggg
     2341 cggttgcggg ggtggagaaa gcgggggctc agggacgctc aacgcgctgt tcatgaaaca
     2401 ggccgcctac agcgacaccg acgtcaagag catgaccgag cagttccaga agcagaatcc
     2461 cggcatcaag gtaaacctca cgttcgtggc ctacgaagcg ctgcacgaca agatcgtggc
     2521 cgcggctccc gccgggacgt acgacaccgt gctggtggac gtcatctggc ctgccgagtt
     2581 cgccagcaag aagatgatag tggacatcac cgaccgcttc cccgagagcg agcggagcaa
     2641 gatcttcgcc ggtgcactga agacaaccga gtacgacggc cgttactacg gtgtgccgtg
     2701 gatactggac accaagtatt tcttctacaa caagaagatg cttggggagg ctggagtgag
     2761 cccgccggat acctgggacg gggtagtgga ggccgcgcgg gccatcaagt ctaaaggggt
     2821 tgtggagtat ccgctcatat ggagctgggc tcaggccgaa gccctcatct gcgactacac
     2881 cacgctcctg ggagcctacg ggggtcagtt cttcgacgag agtggcgagc ccgcgttcaa
     2941 tagcggcggg ggacttcagg ctctcgagtt catgcgcatg accctggacg agaaactctc
     3001 caacccggcg tccacggagt ctctggagga ggacgtgcgc cggatcatat cccagggtga
     3061 agcagctatg gcgctcaact ggacgtacat gttcgccctc gccaacgatc ccaacgagag
     3121 ccaggtagcg ggccagatag acatagccca cacgccggaa ggaccggcgg gggctctggg
     3181 tgtgaacgga agcatgggca tagccatcgc caacggcagc cagaatcagg acgcggcctg
     3241 gaagtacata cagttcatga ccagccagaa gatacagaac cagtacgcga agctgtcttt
     3301 gcccatctgg aagagctcct atgagcagga agaggtcatc caggcgctac cgcaggtcgt
     3361 gccgatcgcc aaagtgcagc tgaacgacat gatcctgcgc cccgtcgtca cgaactacaa
     3421 cgacgtctcg cacaccttgc aggtggagat acagcaggcg ctgaccgggg aaaagtcccc
     3481 gaagcaggcg ctcgacgacg cggcgagcgc cgtggccgag acccagcagt catgagccat
     3541 gggcgtgccg agaagctgaa gaagctaccg cgcgccgttt tcccaagtgg aggagcgttc
     3601 cggcagggga cgactgccct gatcctcctg ctgccaagcc tcgtcgtagt cttcgggatc
     3661 gtcctctacc ccctgctgcg cacgctctac atctccctgt tcgacgttaa cagcgcgttc
     3721 ccagggagtt accctttcgt ggggctcggg aattacatgg aggctctggg gagaacggag
     3781 ttctgggccg cgatactgcg caccgcgtat ttcaccctct ccacgaccct gctcgaggtg
     3841 gtgctcggca ttctgctggg gttgctgctc aacgccgagt tcagggggcg gtggctactc
     3901 aggtccatcg tgatcctccc gtgggccttg ccgacggtcg tcaacggggc aatgtggcgc
     3961 tggatcttta accccgcgta tggggctttg aacgccctgc tcacccagct ccacatcatc
     4021 cccgagtacc gctcgtggct cgggacaccg tggatggccc tcaacatggt agtagtggcc
     4081 gacgtctgga agatgactcc tctggccgcc ttcttcatcc tggccgcgct gcagaccata
     4141 ccgcgcgagc tctacgaggc cgcctgggtg gacggggccg gaaggctccg cgccttcttc
     4201 agcataacgt taccgctttt ggtcccgacc atcgtcatca tcctggtctt gaggacgatg
     4261 gaggcgttca aggtcttcga cattatctac gtcatgaccc gcggcggacc cgccgacggg
     4321 acccagacga tagcctatta cacctacgtg gaggcgttct ccaaccagtt attcgggtac
     4381 ggggcggcgc tggcgtacgt gatcgcgctc ttcatcctgt tcttcgccat catctatatg
     4441 cgactgctgc gtcgcgggga ggtggagtac tagccgatgc gaaactctac gggattcaaa
     4501 gtcttcatct acgctgccgc ggtgttcgtg agccttatca tcctggcccc gttcgcctgg
     4561 ctcataatct ctagcgtggc ggccccggcc gacctgttga ccaaacccct gcgttggatc
     4621 ccgccccacg cctcgctctc ccgctactcc gcaatcttct tcggcgccaa cagcgagacg
     4681 gtcgtgacgt tccgggcggc gttgttgaac agcaccatcg tcgccacgag ctccgtcctc
     4741 atctcgctcg gggtaggtat cttcggggct tacgccttcg cccggctcca gttcagattc
     4801 cagaggggga tactgctggt ctttctctcc acgtacatgc tgccgcccat cgccatactg
     4861 atctcgctct acctcacgat ggcgcagctg cacctcctgg ataccaggct cgggctcatc
     4921 ctggtctact gctccttcat cacgccgttc gtgttgtgga tcatgagcgg gtacttccgg
     4981 agcataccgc gggatctgga agatgccgcc cgtatcgacg gctgcagccg catcggcgcg
     5041 ctatttcgca tagtgcttcc cctggcccgt cccgggatcc tggcgaccgc cctcttcggc
     5101 ttcttgctgg cgtgggacga gttcctctac gccctggtct tcacctcatc cctgagctcg
     5161 aagaccatcc ccgtcgccat cgccgagttc acaggccgcc atgctgtgga cttcggcatg
     5221 atggcaaccg gcggcgttct ggcctcgctg cctccgctcc tgatcgcctt cgtgttccag
     5281 cgctacatcg tcgggggtct cgcctccggc gcggtgaagg gctgaacgtg ggattcctgg
     5341 aagacatact ggagcaaccg cagaacctgg cccgctctcg cgaaatcttc acgcaagccc
     5401 tgcgtggcgc ggacctctcg gccttcgagg cggacccgct cgtcctggcc ggtatgggtt
     5461 ccagcctctt cggcgcgata cccgcggctt gcgctctgcg cggcgcagga cgtgcggctt
     5521 tcgccctctc cgctaccgag cttctcgagc ctggcgggaa cctcctgggc aaggcttacg
     5581 tcggaatctc ccagtccgga aagagcgcgg agacggtaga ggccctctcg agggtgtccg
     5641 ccccgcgact ggcgttgacg aacacgggag cagggcctct ctcggaggtc gccgatgtgg
     5701 ccctgccgat agggtctgcg gaagacacgg ccatcgccgt gctcacctat accgccacgc
     5761 tcgccgccac cggcatgcta gccggcgctc tcggtgctcc tctgggcttc gactgggacc
     5821 ggctaccgga cctggtaggg aaggtgctgg aggacggcgc gcgtgcggcg gaggacttcg
     5881 cggagcgatt cgacgggatg gagttcctcg acttcgtggg tcggggaagc tcccttgcct
     5941 cggcggccga gggagcgcta ctcgcgaggg aggcggtcag gcttccggcc gcatgcctgg
     6001 ataccctcca gtacctccac ggtccgctcg aggtggccga gccaggtcgc gggtgtgtcc
     6061 tcttcggctc cgggcgagag gtacagctcg ctgcggacct cgcctcctac ggggcttcgg
     6121 tgctgctcat tagccgagcc tctatgccct ccacgcggaa cctgaaggtc ttccgcat
//
LOCUS       DASTUV010000426         5272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1325334, whole genome shotgun sequence.
ACCESSION   DASTUV010000426 DASTUV010000000
VERSION     DASTUV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325334"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFT03_08595"
     CDS             <1..579
                     /locus_tag="VFT03_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4848243.1"
                     /translation="RTILEFGGVDIVVSNAGLASSAPIEETSVELWDRNHEVLAKGYF
                     LVSREAFKIMKDQGVGGNLIFVASKNALAAGKNASAYSSAKSAELHLARCLAEEGGAD
                     RIRVNTVNPDAVLQGSRIWDSGWREERARAYGIVPDELEDYYRQRNVLKVNVLPENVA
                     EAVLHFASEARSSRSTGNVLNVDGGVKDAYPR"
     gene            595..1266
                     /locus_tag="VFT03_08600"
     CDS             595..1266
                     /locus_tag="VFT03_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU4848244.1"
                     /translation="MNHQLLREEVVRVSRRMSESGLVMGTSGNVSARTPDGNVLVTPS
                     GLDYAAMDPEDVVLVDLEGAVVEGSFEPSVETPMHTGIYRARPETGGIVHTHARYSTT
                     LACLNWEIPPIHYMLAVLSDEGRVPLARYATYGTVALARNAAEALGSSHAACLLSNHG
                     TIAVGGSVSEAYSRTELLEEMAELYFRARTAGEPVILTHGQMAEVSAKIHDYGQHKPS
                     PSRTE"
     gene            1263..2429
                     /locus_tag="VFT03_08605"
     CDS             1263..2429
                     /locus_tag="VFT03_08605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4848245.1"
                     /translation="MNAADVIVIGAGVVGASVAFHLAERGVDTLVLDREGPAAGSTAR
                     SGALIRAHYPTALEADLAWESLTDYFELWGERVGGGCGFTRTGFAYLVADENVEALRH
                     NVALQQGVGVETGIVGPDELGEIDPALRTTGVSLAAYEPRGGYADPTATAVGFLRAAK
                     TLGARFERRRATALLVSGDRLRGVRTEDGPVESGAVVLAAGAWSVPIAATGGLDLPIR
                     PARVRVALFERPYALPTHLTLIDTTEGFYARPAAEQTTLVGSRDSLEWLGSPDAPTPE
                     PDGTFTVEALRKVAQRLPALAQAPYRSGRSGILDMTPDGRPLLGPGGPEGLYLAVGWS
                     GTGFKKSPAVGAEVARWVSEGAPSRPELESYDLARFDRGARIQGRYEYAVSGPH"
     gene            complement(2426..2848)
                     /locus_tag="VFT03_08610"
     CDS             complement(2426..2848)
                     /locus_tag="VFT03_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEU4848246.1"
                     /translation="MERETGNYGVQREHERWLAPYGPIGGTMSARDERTWSALAHLST
                     FLNAFTGFLGPIVAFVIWLVHRDKSPTVAHHALRSVFYQTAWLVAIVVGWTITTALMA
                     VLIGFLLVPVMIVVTLAPFVQASYEAYMAYIGEGRRYV"
     gene            3039..4376
                     /locus_tag="VFT03_08615"
     CDS             3039..4376
                     /locus_tag="VFT03_08615"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEU4848247.1"
                     /translation="MTGESRGMENGGGMTITGTESLSVKDNRTGKGYDIDVKDGTVRA
                     MDFRQVKVDEEDFGLMTYDPGYSNTASCRSAITYIDGEKGILQHRGIPIEQLCEQSTY
                     LEVAYLLIYGHLPTEDEYNRWVYEITHHTYVHENIKNFMDGFRHDANPMGMLLASVGA
                     LSTFYPEAANIDDEFQRHVSAVRLIAKMPTLAAFSYRHSLGLPYVYPDNDLSYTGNFL
                     SMLFKMAEPRYEPDPRLEKALDVLFILHADHEQNCSAAAVRVVGSSRVDPFSAIAAGV
                     AGLYGPLHGGANVAVLRMLQRIGSTDNIVDFLEGVKRGDERLMGFGHRVYKNYDPRAR
                     IIRKHVDEVLEATNKESPLLDIAVELEKRALDDDYFTSRKLYPNVDYYSGLIYEALNI
                     PTDAFTVMFAIPRTSGWVAQWVEMLHDPELKIARPRQIYTGPRDVDYVPMSKR"
     gene            complement(4482..>5272)
                     /locus_tag="VFT03_08620"
     CDS             complement(4482..>5272)
                     /locus_tag="VFT03_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU4848248.1"
                     /translation="ARRWPALRDIPWFPAAGDGACSIVGCGCTRNDRLALMIGTSGAM
                     RVLWKADSVEIPDGPWCYRADEKRFVMGGALSDGGNLVAWLRETLRLPEETERLLAGM
                     KPDSHGLTFLPLLAGERGPGWADEANGTIAGLSMSTTPVEILRAAMEAVALRFALIAE
                     IIEQASPGEKEVIATGGGLLGSPTWTGIMADALGRPVITSGIHEASSRGAALLALERL
                     GELEIEDAEAPLGKVFDPDPERYEVYREALARQRRLYDAVLGAS"
BASE COUNT          960 a         1761 c         1683 g          868 t
ORIGIN      
        1 cggaccatcc tggaattcgg cggggtagac atcgtcgtct cgaacgccgg cctcgcctcc
       61 agcgccccta tcgaggagac gagcgtcgag ctgtgggaca ggaaccacga ggtcctcgcg
      121 aaggggtact tcctggtctc acgcgaagcg ttcaagatta tgaaggatca gggggtaggc
      181 ggaaacctca tcttcgtggc ctccaagaac gctctggccg ccggcaagaa cgccagcgcc
      241 tattcctcgg cgaaatctgc ggagctgcat ctggcgcggt gtctggcgga ggagggtgga
      301 gccgaccgca tccgggtcaa cacggtaaac cctgacgcgg tgctccaggg ctccaggatc
      361 tgggactccg gctggcgcga ggagcgcgcg cgagcctacg ggatagtgcc cgacgagctc
      421 gaggactact acaggcaacg caacgtgctc aaggttaacg tgctccccga gaacgtcgcc
      481 gaggccgtgc tgcacttcgc ctccgaggcg cgctcctcga ggagcacggg caacgttttg
      541 aacgtggacg gcggggtcaa agacgcctat ccccgctaga tggagaggag gcacttgaac
      601 caccagcttc tccgggaaga ggtagtgaga gtctcccggc gcatgagcga gtcgggtctc
      661 gtcatgggaa cctccggcaa cgtcagcgcc cggacccccg acggcaacgt gctcgtgaca
      721 ccgagcgggc tcgactacgc ggctatggac ccggaagatg tagtgcttgt agatctcgaa
      781 ggtgcggtgg tggaaggctc cttcgagccc tccgtcgaga ccccgatgca caccggcatc
      841 taccgcgcca ggcccgagac cggcggcata gtccacaccc acgcccgtta ctcgactacc
      901 ctggcatgcc tgaactggga gattcctccc atacactaca tgctcgccgt gctctccgac
      961 gagggacggg tgcccctagc ccgctacgcc acgtacggca ccgtggcgct cgcgcgcaac
     1021 gctgccgagg cactcggctc ctcgcacgcg gcctgcttgc tcagcaacca cggcaccatc
     1081 gctgtcggcg ggtccgtgag cgaggcgtac tcccgcaccg agcttctcga ggagatggcg
     1141 gaactctact tccgcgccag gaccgccggg gagccggtca tccttaccca cgggcagatg
     1201 gccgaggtct cggccaagat ccacgactac ggccagcaca aaccctcgcc ctccaggaca
     1261 gaatgaacgc ggcagatgta atcgtgatcg gggctggggt ggtgggcgcg agcgtcgcct
     1321 tccacctcgc cgagcgcggc gtagataccc tggtgctcga ccgcgaaggt cccgccgccg
     1381 gctcgactgc gcgttccgga gccctgatcc gggctcacta ccctaccgcg ttggaggcag
     1441 acctggcctg ggagagcctc acagactact tcgagctgtg gggtgagcga gtcgggggag
     1501 gctgtggctt cacccgtacg ggtttcgcct acttggtcgc agacgagaac gtcgaagccc
     1561 tgcgccacaa cgtcgccctg caacaagggg tcggagtgga gacgggcatt gttggccccg
     1621 acgagctggg cgagatagac cctgcgctcc gcacgaccgg tgtctctctc gccgcctacg
     1681 agcctcgcgg cggctacgcg gatcctaccg ctacagccgt aggctttctc cgggcggcaa
     1741 agacgctcgg agcgcgcttc gagcgtaggc gcgcgacggc tctactcgtc agcggggacc
     1801 gcctcagggg agtccggacg gaggacggcc ccgtggagtc gggtgccgtg gtgctcgcag
     1861 ccggggcgtg gtccgtgccg atcgccgcga ccggcggtct ggaccttccc atccggccgg
     1921 ccagggtgcg ggtcgcgctc ttcgagcgtc cttacgcgct accgacgcac ctcaccctga
     1981 tcgacactac agagggcttc tacgcgaggc ccgccgccga gcagaccacc ctcgtcggca
     2041 gccgggacag cctggagtgg ctgggtagcc ccgacgctcc aacgccagag cccgacggga
     2101 cctttaccgt ggaagccctg cggaaggtcg cgcagcgcct ccccgcgctc gcgcaagccc
     2161 cgtaccgctc gggccgctcg gggatactgg acatgacccc ggacgggcgt ccgctactcg
     2221 ggcccggtgg gcccgaaggt ctctaccttg ccgttggctg gagcggtacg gggttcaaga
     2281 aatcgccggc ggtcggggcc gaagtggcgc gctgggtctc ggagggcgcc ccgagcaggc
     2341 cagagcttga aagttacgac ctcgcccgct tcgatagggg agccaggatc cagggccggt
     2401 acgagtacgc cgtctcgggc cctcactaga cgtagcggcg accctcgcca atgtaggcca
     2461 tgtacgcctc gtaggaggcc tggacgaagg gcgccagcgt caccacgatc atcaccggta
     2521 cgagcaggaa cccgatcaag accgccatca acgccgtcgt gatggtccac ccgacgacga
     2581 tggcgacgag ccacgccgtc tggtagaaga cggacctgag cgcgtggtgg gccaccgtcg
     2641 ggctcttgtc cctgtgcacg agccagatta cgaatgccac gatcggcccg agaaagcccg
     2701 tgaaggcgtt caggaaggtg cttagatggg ccagcgccga ccacgtgcgc tcgtcccgcg
     2761 cgctcatcgt tcctccgatc ggaccgtagg gcgcaagcca ccgctcgtgc tctcgctgga
     2821 cgccgtagtt gcctgtttct ctctccatgc atgctccttg tagccgctct ccaactcaca
     2881 tacgagcccc ctacacgaag gtttcaccag cccacttgaa cgctccactt tccgtttacc
     2941 cacgggtaag cgataatgat agagcttcga aattacatct catgcatcta tatggcacga
     3001 gaatatataa tgagtgcagc cagagaaagg ggcgcaggtt gaccggagag tctcggggca
     3061 tggagaacgg tggtgggatg accatcacgg ggacggaaag cctatctgtc aaggacaacc
     3121 gcacgggcaa gggctacgac atagacgtca aggatggcac ggtccgggcg atggatttca
     3181 ggcaggtcaa ggtcgacgag gaggacttcg gcctgatgac ctacgaccct ggctactcga
     3241 acacggcgag ttgccggagc gccatcacct acatagatgg ggagaagggc atcctgcagc
     3301 acaggggcat cccgatcgag caactctgtg agcaatcgac ctatctggag gtagcgtacc
     3361 ttctgatcta cggtcacttg cctaccgagg acgagtacaa caggtgggtc tatgagatca
     3421 cccaccacac ctacgtccac gagaacatca agaacttcat ggacggtttc cggcacgacg
     3481 ccaacccgat gggtatgttg ctggcttctg taggggcgct ctcgaccttc taccccgagg
     3541 cggccaacat cgacgacgag ttccagcggc acgtatcggc ggtgcgcctc atcgccaaga
     3601 tgccgaccct ggccgccttc tcctaccggc acagtctcgg cctgccatat gtctaccccg
     3661 acaacgacct ctcgtatacg ggcaatttcc tctccatgct cttcaagatg gccgagcccc
     3721 ggtacgagcc tgacccgagg ctcgagaagg cgctcgacgt gctgttcata ctgcacgccg
     3781 accacgagca gaactgctcc gcggccgccg tcagggtcgt cggctcctcg cgggtggacc
     3841 ccttctccgc gatagctgcc ggcgtggccg ggctgtacgg gccgctgcac ggcggggcca
     3901 acgtggctgt cttgaggatg ctccagcgga taggctcgac cgacaacatc gtggacttcc
     3961 tcgagggcgt caagcggggc gatgagcggc taatgggctt cggccacagg gtctacaaga
     4021 actacgaccc gcgcgccagg atcatccgca agcacgttga cgaggtgctc gaggcgacca
     4081 acaaggagag cccgctcctg gacatagccg tcgagctcga gaagcgggcg ctcgacgacg
     4141 actacttcac gagccgcaag ctctacccca acgtggacta ctactcgggg ctcatctacg
     4201 aggccctgaa catcccgaca gacgccttca ccgtgatgtt cgccataccg cggacctccg
     4261 gctgggtggc ccagtgggtc gagatgctcc acgaccccga gctcaagatc gcgcgcccgc
     4321 gccagatcta caccggcccc cgcgacgtgg actacgtgcc gatgagcaag agatagatct
     4381 cagactccag gtaatttggc ctcggtagcg cgtgcgtcgc gtttgtgggg gctttcggag
     4441 gcgaacgaga gcctctccat aacccctgtt gcctgaggcc tttaagatgc tccgagcacc
     4501 gcgtcataga ggcgtcgctg gcgcgccagg gcttccctgt agacctcgta ccgctcgggg
     4561 tcggggtcga agaccttgcc gagcggggcc tccgcgtcct cgatctccag ctcgccgagt
     4621 ctctcgaggg ctagcagagc tgcccctcga ctcgaggctt cgtggatgcc ggaggttatg
     4681 acgggcctgc cgagggcgtc ggccatgatc ccggtccacg tcggcgagcc gaggagcccg
     4741 ccgccggtgg cgatgacctc cttctcgccc ggcgaagcct gctcgatgat ctccgcgata
     4801 agggcgaacc gcagggctac cgcctccatg gcggcgcgca atatctccac tggcgtggtg
     4861 ctcatcgaga gcccggcgat cgtcccgttg gcttcgtccg cccatccagg cccacgctcg
     4921 cctgccagca gcggtaggaa ggtgagaccg tgcgagtcgg gcttcatgcc cgccagcagc
     4981 cgttcggttt cctcagggag acgcagcgtc tctcgcagcc acgcgacgag gttcccgccg
     5041 tccgagagcg cgccgcccat cacgaacctc ttctcgtccg ccctgtagca ccacggcccg
     5101 tccggtatct cgacggaatc ggctttccag agcacgcgca tcgcgccgct agtgccgatc
     5161 atgagcgcga gccggtcatt ccgcgtacat ccgcacccga cgattgagca ggctccgtcg
     5221 cccgcagccg ggaaccacgg gatgtcgcgc aatgcgggcc accgccgggc cc
//
LOCUS       DASTUV010000427         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1709475, whole genome shotgun sequence.
ACCESSION   DASTUV010000427 DASTUV010000000
VERSION     DASTUV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709475"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFT03_08625"
     CDS             <1..316
                     /locus_tag="VFT03_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848249.1"
                     /translation="RELGVEAVRELNSWAVETVVPDRTFYLRLGAEERARRALRLGAP
                     LDRIEKVGDEFMRRVEEGFEELVRREPHRIEVLDAGRSSEELAELVVGEIRSLQYTRR
                     GG"
     gene            334..1473
                     /locus_tag="VFT03_08630"
     CDS             334..1473
                     /locus_tag="VFT03_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848250.1"
                     /translation="MADRAASFSGIVGNEAALGLLERALESGEVSHAYLFYGPPGVGK
                     RTAARRFGSALVAGGDSGAEERARRGLHPDLSEVEPEGAFTTIGQVREVVRLAASRPF
                     EGARRFFILQADTLNVQAANALLKTLEEPEGETVFALLAASREGVLPTILSRAQAVRF
                     NPVPQRTVAKLLADRGAAEPELAAALGRGSVGLSLRYAGEPELRELRDAVFEAGFSLS
                     RDFEERSRMVGKITAQAEAVGAAREASFLEEIEEPDRRAREGAKRAGRAARDGAVREA
                     LELLALAYRDAAAVGAGAPELVANVDREEEIRRKVEEHPEADWAGAALVLGEARAALA
                     YNVSPEAILEVALSRIRRRILTPSPGSSTSVSRAGARRGSATPGS"
     gene            1428..2279
                     /gene="ricT"
                     /locus_tag="VFT03_08635"
     CDS             1428..2279
                     /gene="ricT"
                     /locus_tag="VFT03_08635"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEU4848251.1"
                     /translation="MPGWSTPRLCYARELDLRVGYKVVVATEVGEFVGRVALTPRRRE
                     AYTEPYDILRIVTEEDAREDDRNRERGREIRTEAQKLARDHGIGKVQFIGCDVSLDGY
                     YVEVKYQTDENNLNLDPVTEGLERLYDARIALRRFRFIERSGDTSGCDTCGLPLCCAT
                     WSGARSMGPVNVRLAKQQGVTPNEKIMGCCGEVKCCVRYEHDVYKEFKERAPFKNSTV
                     RLGEREGKVVDYSMVKDSVFVRFGPTRAEQELIPLGRLARDNPGVFPADPEEWQETPE
                     ASQHQDS"
     gene            2286..3248
                     /locus_tag="VFT03_08640"
     CDS             2286..3248
                     /locus_tag="VFT03_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU4848252.1"
                     /translation="MSGNLCDVPGVLVGHATDPVALTGCTAVLFEDPEGATVGVDVRG
                     SSPGTRETDRLGPHGTVRKTHALLLTGGSAFGLAAAEGVVSYLEENGIGLDIGVTRIP
                     LVSAAVLFDLVVGDPAVRPDHSMGYEAADSASSRDFEQGSVGAGTGASVGKVLGMERA
                     MKGGVGSVSIRLDRGLVVAALAAVNAFGDIRDPRTGRVIAGPRHDDGTLGDTVELLQE
                     AASRHRWEENTTLGIVATNARLTKSGANKVAQMAHDGFARVIEPVHTTLDGDVAFAIS
                     AGDVEAETDVVGAWGARAMQEAILRAVRTAKGVPGIPSLSEYED"
BASE COUNT          603 a         1083 c         1325 g          502 t
ORIGIN      
        1 gagggagctc ggcgtggagg ctgttcgcga gctcaactcc tgggctgtgg agaccgtcgt
       61 ccctgacagg acgttctatc tacggttggg cgcggaggaa cgcgcgcggc gcgccctgag
      121 gctgggggcg ccgctcgaca ggatcgagaa ggtgggtgac gagttcatgc ggcgggtaga
      181 ggaggggttc gaagagctgg tccggcgcga gccgcacagg atcgaggtcc tcgacgcggg
      241 gcggtcttcc gaggagctgg ccgagctggt ggtgggtgag atccggagcc tgcagtacac
      301 tcgtcgtggt gggtgatcag cggacgaaat ctgttggctg atagggccgc gagcttctct
      361 ggcatcgtcg gcaacgaggc ggctctgggg ctgctggaga gagcgctgga gagcggggag
      421 gtctcccacg cctacctctt ctacggtccg cccggggtag gcaagcggac ggcggcgcgg
      481 aggttcggct cggcgctggt ggcgggcggg gactcgggcg cggaggagcg tgcgcggcgc
      541 gggctgcacc ccgacctctc ggaggtggag ccggagggag cattcacgac gatcgggcag
      601 gtcagggagg tggttcgcct cgcggcgagc cggcccttcg aaggagcgcg ccgcttcttc
      661 atcctgcagg cagacacgct caacgtgcag gccgccaacg cgctgttgaa gacgctggag
      721 gagccggaag gggagacggt tttcgcgctg ctcgctgcct cgcgcgaggg cgtcctgccg
      781 accatcctct ctcgagcgca ggcggtacgc ttcaaccccg tgccgcagag gacggtggca
      841 aagcttcttg cggatcgtgg cgctgcggag ccggagctcg ccgctgcgct ggggcggggg
      901 agcgtagggc tctcgctgcg ctacgccggg gagcccgagc tcagggagct gcgggacgcc
      961 gtcttcgagg ccggattctc tctctcacgg gacttcgagg agcgcagccg gatggtgggg
     1021 aagataacgg cgcaggccga ggccgtgggg gctgcgaggg aggcgtcgtt tctggaggag
     1081 atagaggaac ccgaccggag ggccagggag ggtgcgaagc gcgccggccg ggcggcgcgg
     1141 gacggagcgg tgcgggaggc gctggaactg ctcgcgctgg cgtacaggga cgcggctgcg
     1201 gtcggggccg gagcgcccga gctagtggcc aacgtggatc gggaagagga gatccggcgc
     1261 aaagtggagg agcatccgga ggcggactgg gccggggcgg cgctggttct aggggaagcg
     1321 cgagccgcgc ttgcgtataa tgtttccccg gaggcgatcc tggaggtggc gctatcgcgg
     1381 atacggcgga gaatactcac accctcccca ggctcgtcga catccgtatc ccgggctgga
     1441 gcacgccgag gctctgctac gccagggagc tagacctcag ggtcggctac aaggtcgtcg
     1501 tcgcgaccga ggtaggggag ttcgtcgggc gcgtcgccct cacgccgagg cgccgcgaag
     1561 cgtacaccga gccctacgac atactccgca tcgtcaccga ggaggacgcg cgggaggacg
     1621 accggaacag ggagcgggga cgcgagatcc gcaccgaggc ccagaagctc gcccgcgacc
     1681 acggtatagg gaaggtccag ttcatcggct gcgacgtctc gctcgacggc tactacgtcg
     1741 aggtaaagta ccagaccgac gagaataacc tgaacctaga tccggtcacg gaaggtctcg
     1801 aacggctcta cgacgcccgg atcgcgctgc ggcggttccg gtttatagag cgctcgggtg
     1861 atacgagcgg gtgcgacacg tgcgggctgc cgctgtgttg cgcaacctgg agcggggcgc
     1921 ggagcatggg gcctgtcaac gtgcggctcg ccaagcagca aggcgtgacc ccgaacgaga
     1981 agatcatggg ctgctgcggc gaggtgaagt gctgcgtgcg ctacgaacac gacgtctaca
     2041 aggagttcaa ggagcgcgcg cccttcaaga actcgaccgt caggctcggt gagcgtgagg
     2101 gcaaggtcgt cgactactcg atggtgaagg actccgtctt cgtgcggttc ggcccgaccc
     2161 gcgccgagca ggagctcatc ccgctcgggc gcctggcccg cgacaacccc ggcgtgttcc
     2221 ccgcggaccc cgaggagtgg caggagacgc ccgaagcctc gcaacaccag gactcgtgaa
     2281 gccccgtgtc cggcaacctc tgcgacgtgc ccggcgtgct cgttggccac gccaccgacc
     2341 ccgtggctct gaccggctgc acggccgtcc tcttcgagga ccctgagggt gccacggtcg
     2401 gcgtcgacgt gcgcggctcc tcgcctggta cgcgggagac ggatcgcctc ggcccccacg
     2461 ggacggttcg aaagacccac gccctcctgc tgaccggagg aagcgccttc ggcctcgcag
     2521 ccgccgaagg agtcgtcagc tacctggagg agaacggcat cgggctcgac ataggggtga
     2581 cccgcatccc gctggtctcc gccgcagttc tcttcgacct cgtcgtcggg gatcccgcgg
     2641 tccgccccga ccactcgatg ggctacgagg ccgccgactc ggccagcagc agggacttcg
     2701 agcagggcag cgtaggcgcg ggcacgggcg cgtccgtagg caaggtcctc gggatggagc
     2761 gggctatgaa gggtggagtc gggagcgtgt ccataaggct cgacagaggt ctcgtcgtcg
     2821 cggctctcgc agccgtcaac gccttcggcg acatccgcga cccgaggacg gggcgtgtga
     2881 tcgccggccc aaggcatgac gacgggacgc tcggggacac ggtggagttg ctccaggagg
     2941 ccgcctcccg gcatcggtgg gaggagaaca cgaccctcgg catcgtcgcg accaacgccc
     3001 gtctcacgaa gagcggggcc aacaaggtgg cccagatggc ccacgacggc ttcgcgcgcg
     3061 tcatagagcc cgtgcacacg acccttgacg gagacgtggc cttcgccatc tcggccggcg
     3121 acgtggaggc cgagaccgat gtcgtcggcg cgtggggcgc gcgagccatg caggaggcaa
     3181 tcctgcgcgc cgtccggacc gcgaagggcg tgccgggaat accatcgctc tccgagtacg
     3241 aggactgaag caattcccag gataatttcc cgacgtgcgg cggatcgaca gttgatcccg
     3301 gcagaactcg ggagaggctg cgccccagca aacaacaagc cgaaatgctg agaagcgagg
     3361 ccctaaaagg gccgaagcgt gctgagaagt ccccgcaggg gacgtgctga caagccgacc
     3421 gcagggaggc gtgctttcat catcacagac tatccaggat agcaggcgcg gcctcgacgg
     3481 gctcgtccga gacctcgagg gcctcccgca cga
//
LOCUS       DASTUV010000428         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1513582, whole genome shotgun sequence.
ACCESSION   DASTUV010000428 DASTUV010000000
VERSION     DASTUV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513582"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..545)
                     /locus_tag="VFT03_08645"
     CDS             complement(159..545)
                     /locus_tag="VFT03_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4848253.1"
                     /translation="MPNVKLDHCVIHVSDWEISNTFYRDVLGAELVPRESGWSYRFGD
                     EQLNLHGPGVSPAPVAKLPVQPGNSDLCFEWPGPIDEAREHLERNGVEVEMGPVERSG
                     ARGTGTSVYFRDPDGSLLEFISYYAA"
     gene            565..1059
                     /locus_tag="VFT03_08650"
     CDS             565..1059
                     /locus_tag="VFT03_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4848254.1"
                     /translation="MPRDCTVGRESRRGQRALCSAPRNWEANVTLLASMSLSGIGPSL
                     AVEGATTKAVFEAYVERVLAPELKPGQIMVMDNLSSHKGSRVRKLIEERGCTLLYLPP
                     YSPDLNPIEEAFAKLKALLRRAGVRTREALIEAMGRALDAVTAGDARGFFEHRGYRVA
                     AHLL"
     gene            complement(1099..1548)
                     /locus_tag="VFT03_08655"
     CDS             complement(1099..1548)
                     /locus_tag="VFT03_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848255.1"
                     /translation="MSVVAVFTGVGLLAGWLAVGFEPLAGLGGATLSVGEGVALIWAS
                     AGLVALPLLGLAAFALFLSSATHNSVASVVGGVVLILLLQLVSSLGLGGALDPYLLTN
                     QFAAWLNLVREPIDWAPIIRAAWVSLLWTSGSLLAAWAIFVRRDVLS"
     gene            complement(1552..1899)
                     /locus_tag="VFT03_08660"
     CDS             complement(1552..1899)
                     /locus_tag="VFT03_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4848256.1"
                     /translation="MVRWEAYKLIRQRRTFLGLGAAAIVPVLFLSAILITQQGPDAGE
                     APFAAQLLSNGLVIPFMTLGFGSVVLLPLLTVLVAGDAVAGEVSAGTLKTILGRSVGR
                     SGLFWAKAAAVGL"
     gene            complement(1899..2873)
                     /locus_tag="VFT03_08665"
     CDS             complement(1899..2873)
                     /locus_tag="VFT03_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848257.1"
                     /translation="MDGTREFGGTAAGAVPALELRGLTKRFGNLTAVEDLSLEVGRGD
                     VYGFLGPNGSGKSTTIRMILGLVRPTAGEVRIFGRPATGPAQRANVAGFVEAPGFYGY
                     LSGRDNLRLLAAADPRGDKSPPIQRVLETVDLAGRAKDKVKTYSQGMKQRLAIASALL
                     REPEFLVLDEPTNGLDPGGMRDVRALVRELAGEGLTIFLSSHLLSEVEHLCNRVAVVG
                     KGRLLAEGSLGEIAEQARGRPEYRLVADDRERANHVLREHPDVALLNGRPEELEGFRP
                     DEVIRFAAGSEGVGPVVKALVDGDVEVLALVPARPNLEEMFLRLTEGE"
     gene            complement(2877..4100)
                     /locus_tag="VFT03_08670"
     CDS             complement(2877..4100)
                     /locus_tag="VFT03_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848258.1"
                     /translation="MSSNAGEGRGLARSLVLVLGIAVLLGLAGALVGMRAFAAQAPPE
                     ELSDDELLSRVARATGDPPAFSASITVEQSVLPAQLLEASGQEGGPPALSGPLSARVW
                     YGGPTQLRAELQGENGDRIFVRNGSRVWIYDGAENTVRTGEGVPEQETPDEEPVTPTE
                     VNRLLDELAPTSELSQQEPVEVAGRQAYVLVLSPRDEGATLVDRAQMLVDSETYLPLR
                     FAVYADERPDPVYSYQVSGLDVGPVPADLFDFQTPPGAEVLPLEQGAEPREQERPEGA
                     EPTQVKTVAEAQRLVDFRIRELPDPPGDRELTGVYLKNGDGVVLTYGSGWGTVVFAQG
                     QGDGDAAMPPEAGDAEANGLQQLPTVDLGGGVEAQEISTPIGSGLSWSADGVGYVLAG
                     SVPASELEQAARGLR"
BASE COUNT          733 a         1545 c         1479 g          723 t
ORIGIN      
        1 tctgtacttg gtagggtact ggttgaatat ctagccagac ttttcagaca gtctctctag
       61 gaggttggtg aatagcggca ggtggctcct cgcaccacct gactggatcg tgggctagct
      121 atgagggtcc ggtacgttcg tgttgcgtgc gggcacggct acgctgcata gtacgagatg
      181 aactcgagga gggaaccatc cgggtcgcgg aagtagacgc tcgtgccggt acctctggcg
      241 ccggagcgct cgacgggacc catctcgacc tcgacgccgt tacgttccag gtgctccctc
      301 gcctcgtcga taggccccgg ccactcgaag cacagatcgc tgttgcccgg ttgtacgggg
      361 agcttcgcga ccggcgcggg ggaaacgccg gggccgtgca ggttgagttg ttcgtctccg
      421 aatcggtacg accaaccgga ctctctcggg accagctctg cgcccagcac gtcacggtag
      481 aacgtgttgg agatctccca gtcggagacg tgaatgacgc agtgatccag cttcacattt
      541 ggcatgcgct gctcctcttg acgcgtgccc cgcgattgta ccgtcggacg cgagtcgcgc
      601 agaggtcaac gagcgctttg ttcggcgccg cgcaactggg aagcgaacgt cacgctcttg
      661 gcgagcatga gcctctcggg gatcgggccg tctctggcgg tggagggtgc aaccacgaag
      721 gccgtcttcg aggcctatgt ggagcgggtc cttgctcccg aactgaagcc ggggcagata
      781 atggtgatgg acaacctctc ttctcacaaa ggttctcggg tgcgcaagct catcgaggag
      841 agaggctgca cgcttctcta cctgccgccc tactcgcccg atctcaaccc catagaagaa
      901 gcgtttgcca agctcaaggc gctcctgagg agggccgggg tgcgtacccg cgaggcgctc
      961 atagaggcga tgggccgagc tctcgacgcg gtgacggcag gcgacgctcg ggggttcttc
     1021 gagcatcgcg gctaccgcgt agcagcccat ttgctatgac agacgctcta agaacctaac
     1081 accctcaaaa cccgaaacct aactcaacac gtccctgcgg acgaagatag cccaggccgc
     1141 gagcagcgaa cccgaggtcc agaggagact cacccacgcg gccctgatga tgggggccca
     1201 gtctatgggt tcgcgcacga ggttgagcca ggcggcgaac tggttggtga gcaggtaggg
     1261 atcgagcgcg ccgccgaggc cgaggctcga gacgagctgg agcagcagga tcaggacaac
     1321 ccctccgacc accgacgcga cgctgttgtg ggtggcggag gagaggaaga gcgcgaaggc
     1381 cgctaagccc aggagcggca acgccacgag gccggcgctg gcccagatga gggctacgcc
     1441 ctcgccgacc gagagcgtcg cgccgccgag gcccgccagc ggctcgaagc cgaccgcgag
     1501 ccacccggca agcaggccga ccccggtgaa gaccgcgacg acggacaaag tctagagccc
     1561 gacggctgcc gccttggccc agaagaggcc ggatctcccg accgagcggc ccaagatggt
     1621 cttgagggtg ccggcggata cctccccggc tacggcgtcg ccggcgacga gcacggtcag
     1681 caggggcagc agcacgaccg atccgaaccc gagggtcatg aacggtatga cgaggccgtt
     1741 ggagaggagt tgggccgcga acggggcctc cccggcgtcg gggccctgct gggtgatcag
     1801 gatcgccgac aggaagagca ccggcactat cgccgccgcc ccgagcccga ggaacgtccg
     1861 ccgctgcctg attagcttgt atgcctccca gcggaccact actcgccctc cgtgagcctg
     1921 aggaacatct cttccaggtt cgggcgggcg ggcactagcg cgagcacctc cacgtcgccg
     1981 tccaccagcg ccttcaccac cgggcccacc ccctcggaac cggcggcgaa ccttatcacc
     2041 tcgtcgggcc tgaacccctc gagctcctcg ggacgaccgt tcaggagcgc aacgtccgga
     2101 tgctctcgca ggacatggtt cgccctctcc ctgtcgtcgg ccacgaggcg atactcgggg
     2161 cggccgcgcg cctgctcggc tatctctccg aggcttccct cggccaggag gcgccccttg
     2221 ccgacgacgg cgaccctgtt gcagaggtgc tcgacctcag agaggaggtg gctggagagg
     2281 aagatcgtga gcccctcgcc ggcgagctcg cgcaccagcg ccctgacgtc gcgcatgccg
     2341 cctgggtcca ggccgttcgt cggctcgtcc aggacgagga actccggttc ccggaggagc
     2401 gccgacgcta tcgccagccg ctgcttcatc ccctgcgagt acgtctttac cttatccttc
     2461 gccctgcccg ccaggtccac ggtctcgagc acccgctgta tcggcgggga cttgtccccg
     2521 cgcgggtcgg cggccgccag gagcctgagg ttgtccctgc ccgagaggta cccgtagaag
     2581 ccgggcgcct cgacgaagcc ggcgacgttc gcccgctgcg cgggacctgt ggcggggcgg
     2641 ccgaagatgc ggacctcccc cgcggtgggc cggacgaggc cgaggatcat gcgtatcgtc
     2701 gtgctcttcc cgctgccgtt cggcccgagg aacccgtaca cgtccccccg cccgacctcg
     2761 aggctcaggt cctccaccgc cgtgaggttt ccgaacctct tggtaaggcc ccgaagctcg
     2821 agcgcgggga cggccccggc cgccgtccct ccgaactcac gtgttccgtc caaggcttac
     2881 cggagcccgc gggccgcctg ctccagctcc gaggccggaa cgctgcccgc cagaacgtac
     2941 ccgaccccgt ccgcgctcca gctcagcccg gaaccgatcg gggtggagat ctcctgtgcc
     3001 tctaccccgc cgccgagatc aaccgtcggg agttgctgga ggccgttcgc ttccgcgtct
     3061 cctgcctcgg gaggcatcgc ggcgtctcca tctccttgtc cctgggcgaa gaccaccgtg
     3121 ccccagcccg agccgtaggt gaggaccacg ccgtccccgt tcttgaggta gacgcccgtc
     3181 agctcgcggt cgcccggggg gtcagggagc tccctgatcc ggaagtccac gagtctctgg
     3241 gcctccgcga cggtctttac ctgcgtgggc tccgcccctt cgggccgttc ctgctcgcgt
     3301 ggctccgcac cctgttcgag cgggagcacc tctgcccccg gcggggtctg gaagtcgaag
     3361 agatcggctg gcacggggcc cacgtcgaga ccggagacct ggtaggagta cacggggtcg
     3421 gggcgttcgt cggcgtagac ggcgaaccgc agcggcaggt aggtctcgga gtctacgagc
     3481 atctgggccc ggtccaccag ggtcgcgccc tcgtccctgg ggctcaggac gaggacgtag
     3541 gcctggcgtc ccgcgacctc gaccggctcc tgctgggaga gctcgctcgt cggcgccagc
     3601 tcgtccagca acctgttcac ctccgtgggg gtcacgggct cctcgtccgg ggtctcctgt
     3661 tcggggacgc cttcgccggt acgcacggtg ttctcggcgc cgtcgtagat ccagaccctc
     3721 gacccgttgc ggacgaagat gcggtcaccg ttctcgcctt gcagttccgc cctgagttgg
     3781 gtcgggccgc cgtaccagac gcgcgcgctc aggggaccgg agagcgcggg cggcccccct
     3841 tcctggcccg aggcctcgag gagctgcgcg gggagcacgc tctgctcgac ggtaatggag
     3901 gcgctgaacg ccgggggatc tccggtggcc cgagccaccc gtgacagcag ctcgtcgtcg
     3961 gacagctcct ccggcggggc ctgggccgcg aaagccctca tccccaccag ggcgcccgcg
     4021 agaccgagga ggaccgcgat ccccagtaca agcaccaggg aacgcgccag cccgcgtcct
     4081 tcgcccgcgt tactcgacat tcgttccgaa cctcctcgta atggagatct gctcaggatt
     4141 acgtgcgtcc cctctagtac gcagcttggt ggcaacgggt ttcgcacggc gcggctcgcg
     4201 gcgaggagcg agactcgccg gggagctaac ctgaaccgcg acgggtctcc cctgccttct
     4261 cccaacggcg tttagcaaca gcatagaggc gatgggccga gctctcttcg aggctctgct
     4321 ccacgtcgcg caagccacca aaccctagca agcccacctg caacgatgcc gcaacgctct
     4381 gctgctaggg tgactcgtac tatgcgtcgc tcgagtaacc acccatttca tcgtgcaggc
     4441 tctcccgggg aactcgctac gggaggtgct accgagacat
//
LOCUS       DASTUV010000429         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1333545, whole genome shotgun sequence.
ACCESSION   DASTUV010000429 DASTUV010000000
VERSION     DASTUV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333545"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFT03_08675"
     CDS             <1..364
                     /locus_tag="VFT03_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU4848259.1"
                     /translation="YGAGTQSSGVVRILKDLEEDITGRHVLLVEDIIDTGLTLSYLRR
                     TLLQREPASLEICALLTKPSRRQVDLRVRYLGFEVPDEFVIGYGIDFAGAYRNLPDIH
                     VLKPEESSLESPPALEKD"
     gene            complement(374..1597)
                     /locus_tag="VFT03_08680"
     CDS             complement(374..1597)
                     /locus_tag="VFT03_08680"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU4848260.1"
                     /translation="MTEQKYFRKGFKIRDDVQHLLDRDYRSEVVERVRENGNVLEIEG
                     LTLRLADEFGFCYGVDRAVDYAFQTREKFPERRIHITGDMIHNASMNDRLREMGIEFL
                     SQGFDGRPDEKFDALGPEDVVLLPAFGAPVDWVRKLKEKECIVVDTTCGSVLSVWKRV
                     TRYAQRGFTSIIHGKYYHEETKATASQTTGEGGNGKYLIVRNLEEVGHVVDFILGRMG
                     SEDLREKLGHGMCPGFDPDEDLQKVGVANQTTMLMSETMAVGEELRKAMVERYGAENL
                     DEHFELFDTICSATQDRQDALFELLEHTLDVAVIIGGYNSSNTNNLAIIAAERVPRSY
                     HIASGDCIIGDTIRHKPAGTPLDAREEVEERGWLPAGPVRLGLTAGASTPNSQIGLAI
                     GRILEARGLPLEAAL"
     gene            1862..2254
                     /locus_tag="VFT03_08685"
     CDS             1862..2254
                     /locus_tag="VFT03_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4848261.1"
                     /translation="MELGKHTVMIADDDPVALATATEALASAGFKVVQATDGQRAIQE
                     ALGRRVDLIVMDVSMPQVGGVEACHCLKAMPKTSKIPVVLTAAKKDPAARMLAERTHG
                     SVRVLRKPFDPDDLVSVAKQLVRPKSLI"
BASE COUNT          423 a          774 c          749 g          480 t
ORIGIN      
        1 ctacggcgcg ggcactcaga gcagcggtgt ggtccgcatc ctcaaggacc tcgaagagga
       61 cataacgggc aggcacgtac tcctcgtcga ggacatcata gacacgggcc tcactctctc
      121 ctacctgcgg cgcacgctgc tccagcgcga gcccgcttct ttagagatct gcgctctcct
      181 gaccaagcct tcgaggaggc aggtggatct ccgcgtgaga tacctcggct tcgaggtgcc
      241 agacgagttc gttataggct acggcataga tttcgccggg gcctaccgca acctccccga
      301 catccacgtc ctcaagcccg aagagagctc cctcgagtct ccgccagccc tggaaaaaga
      361 ctgacaggct gatctagagc gcagcctcga gtggaagccc gcgagcttcc agtatgcgcc
      421 cgatggcgag cccgatctga gagttcgggg tggaggctcc ggcggtgagg ccgaggcgga
      481 ccggtccggc ggggagccag ccccgctcct ctacctcctc gcgggcgtcg agcggcgtgc
      541 cggccggctt gtggcggata gtgtccccga ttatgcagtc gccgctcgct atgtggtagc
      601 tcctcgggac tcgttcggcg gcgatgatcg cgaggttgtt cgtgttcgag gagttgtagc
      661 cgccgatgat cacggcgacg tccagcgtgt gctcgaggag ctcgaagagc gcgtcctgcc
      721 tgtcctgtgt ggccgagcag atggtgtcga agagctcgaa gtgctcatcc aggttctcag
      781 cgccgtaacg ctcgaccatc gccttcctga gctcctcgcc gacagccatc gtctcgctca
      841 tgagcatagt ggtctggttg gcgacgccca ccttctgcaa gtcctcgtcc gggtcgaagc
      901 cggggcacat gccgtggccg agcttctctc tcagatcttc ggaacccatg cggccaagga
      961 tgaaatctac gacgtggccg acctcctcga ggttgcgcac gatcaggtac ttcccgttgc
     1021 cgccttcacc ggtggtctgc gatgctgtgg ccttggtctc ctcgtggtag tacttgccgt
     1081 ggatgatgct cgtgaagccc cgctgggcgt agcgcgtgac gcgcttccac accgagagga
     1141 ccgacccgca agtcgtgtcc acgacgatgc actctttctc cttgagcttc cgcacccagt
     1201 ccacgggagc gccgaatgcg ggcaggagga ccacatcctc gggtcctaga gcgtcgaact
     1261 tctcgtcggg gcggccgtcg aagccctggg agaggaactc tatccccatc tcgcgcaacc
     1321 tgtcgttcat cgaggcgttg tggatcatgt cccccgttat gtggatgcgc ctctccggga
     1381 acttctccct ggtctggaag gcgtaatcca cagcccggtc caccccgtag cagaacccga
     1441 actcgtcggc gagcctcaag gtgagtcctt cgatctcgag gacgttgccg ttctcgcgga
     1501 ccctctcgac cacctccgag cggtagtccc ggtccaggag gtgctgcacg tcgtccctta
     1561 tcttgaagcc cttgcggaag tacttctgct ccgtcacgct cacctctttt tggccgtcaa
     1621 catctcaggc tttacttcaa aagttttcga acttctattc tagcaggcgg tgtcccgagg
     1681 tccgttgaaa gggtcacgcc ccgcaccgaa atgtggtgct tcgaccgggc gcgttccttg
     1741 actttctgaa cgctggtggc tgaggactct tccagaccat cgtgattctg gcgtcggatg
     1801 cttaaaaatg ggtatgtgtg gggatagtta cggatgttaa acaggtgtaa aataggtcgc
     1861 cgtggaacta ggaaaacaca ccgtaatgat cgccgacgac gaccccgtcg cgttggccac
     1921 cgccaccgag gcgctcgcta gcgccggctt caaggtcgtg caggcgacgg acggccagag
     1981 ggcgatccag gaggccctcg gccgcagggt agaccttatc gtgatggacg tctcgatgcc
     2041 ccaggttggg ggcgtggagg cctgccactg cctgaaggcg atgccgaaga cctcgaagat
     2101 accggtggtc ctcaccgcag ccaaaaagga cccggcggcc aggatgctgg cggagcgcac
     2161 acacggctcg gtccgcgtcc tgcgcaaacc tttcgacccc gacgatcttg tctcggtggc
     2221 gaagcaactc gtgaggccca agtcgctgat atagtcctcc gcggcaccag atctcccccc
     2281 gacgcacaga ccactcacgg catgccgctg cctgacccgg cctgcttagg ctcggtgctt
     2341 gaagcggggg acctctggtg acgaagacca tagtcatcgg cggcggtatc ggagggctta
     2401 cggcggccgt cgcgctcggg cgcgcg
//
LOCUS       DASTUV010000430         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1679628, whole genome shotgun sequence.
ACCESSION   DASTUV010000430 DASTUV010000000
VERSION     DASTUV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679628"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFT03_08690"
     CDS             complement(<1..525)
                     /locus_tag="VFT03_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU4848262.1"
                     /translation="MSSEENKRDPELGDDARHEEGAVEEAEGSPVDPASLENGESTGA
                     PEVTEGDPITGDELEWDEATVLSVELEATRRERDQYLDALRRLKAEFENWRKRQERER
                     SRVLSMASERLVQELLPVLDNLDRALEAGGDIREGVQATRDQLAEVLGDEGLLPVVSD
                     GQPFDPNVHEAVMSL"
     gene            complement(603..2507)
                     /gene="dnaK"
                     /locus_tag="VFT03_08695"
     CDS             complement(603..2507)
                     /gene="dnaK"
                     /locus_tag="VFT03_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU4848263.1"
                     /translation="MAKSIGIDLGTTNSCVAVLEGGDPTVITNSEGERTTPSVVAFDR
                     RSGERLVGQLARRQAVTNPERTVYSIKRFMGRRFSDVKTEAERVGYQVKSGPGGDIRI
                     EVDDKEYSPPEISAMTLQKLKRDAEEYLGEEVSEAVITVPAYFEDAQRQATKDAGRIA
                     GLNVKRIINEPTAASLAYGMDKEEDQTILVFDLGGGTFDVSILELGEGVFEVKATSGN
                     NHLGGDDFDAKVVEWMVSEFKKAEGIDLSKDKMAAQRLVEAAEKAKRELSSTTSTSIN
                     LPFITADQNGPKHMDLTLTRAQFNNLTADLVEATAGPVRQAMEDAGLQPGQVDQVILV
                     GGSTRIPAVQEQVKEITGKEPHKGINPDEVVAVGAAIQAGVLAGEVKDVLLLDVTPLS
                     LGIETKGGVFTKLIERNTTIPTRKSEVFTTADDNQGSVEIKVYQGEREIAAYNKIIGN
                     FQLVGIPPAPRGVPQIEVAFDIDANGIVNVGAKDLGTGKEQRITITASSGLSDAEIQQ
                     MMQDAESHAEEDRQRREEADVRNNADNLVYSIERSLKDVDGKVDPNTKLEIEQAIKET
                     KEALAGGDVEEIKRKQENLMTASHKLSEVLYSQAAQQQEASSTGATDTDDDDLVEDAE
                     VVDEDDEERK"
     gene            2687..3376
                     /locus_tag="VFT03_08700"
     CDS             2687..3376
                     /locus_tag="VFT03_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848264.1"
                     /translation="MDETILLVDDDAALLEVTSIILTSEGYRVVTAEDGPEALRILGR
                     EGLDLVVLDIMLPRMSGFEVLKKMREHSDVPVVLLTAKSQSVDKVVGLELGADDYITK
                     PFDTKELLARIKAILRRFGRDNRGREGLLKLGSLELDPNGYTVSRSGEMLDLTPTEFK
                     ILALLMRRPGQAFTRAQISEAVSTGSHYLASRYIDVHISRLRAKVERDPSSPEIVQTV
                     PSVGYRAARPV"
BASE COUNT          567 a         1072 c         1006 g          760 t
ORIGIN      
        1 gaggctcata acggcttcat ggacgttcgg atcgaaaggt tggccgtctg agaccacggg
       61 caggagacct tcgtcgccga gtacttcggc gagttggtcc ctggtagcct gtacgccctc
      121 cctgatatct cccccggcct cgagcgcacg gtccaggttg tcgagaacag gtaggagctc
      181 ctgtaccagc ctctctgagg ccatcgatag tacgcggctg cgttcccgct cctggcgctt
      241 gcgccagttc tcgaactcgg ccttcaggcg gcgcagggcg tccagatact ggtcccgttc
      301 cctgcgcgtg gcctccagct ctaccgaaag caccgtcgcc tcatcccatt ccagctcatc
      361 gccggtgatc gggtcgcctt cggtgacttc tggcgcgcca gtgctctccc cgttttcgag
      421 ggacgcaggg tcgaccgggg agccctcggc ctcctctacc gccccctctt catgccgagc
      481 gtcatccccg agttcggggt ctcgcttgtt ctcctcgctg ctcaaagctc cctaatcctc
      541 ctgtggtcgc cgcgcgggca gccggggtga gtctctacac ccgcggctgc ccgggttcgt
      601 ctttacttgc gctcctcgtc atcctcgtcc acgacctcgg cgtcctcaac caggtcgtca
      661 tcatcggtgt ccgtggcccc ggttgagctg gcctcttgct gctgggccgc ctggctgtag
      721 agaacctcgg agagcttgtg ggaggcggtc atcagattct cttgcttgcg cttgatctcc
      781 tcgacgtcgc cgccggcgag cgcctccttg gtctccttta tggcctgctc tatctccagc
      841 ttggtgttcg ggtccacctt gccgtccaca tccttcagcg aacgctcgat ggagtacacc
      901 aggttgtcag cgttgttgcg gacgtcagcc tcctcacggc gctgcctgtc ctcctcggca
      961 tgcgattccg catcctgcat catctgctgg atctccgcgt cggagaggcc actggaggca
     1021 gtgatcgtga tcctctgctc cttgcccgtc cccagatcct tggccccgac gttgactatg
     1081 ccgttcgcgt ctatgtcgaa agcgacctcg atctggggca caccccttgg ggcaggcggg
     1141 atgccgacta gctggaagtt gccgatgatc ttgttgtacg ctgcgatctc gcgctcgccc
     1201 tgatagacct tgatctcgac cgatccctga ttatcgtccg cagtggtgaa tacctcactc
     1261 ttacgtgtag gtatcgtggt gttccgctcg ataagcttgg tgaacacccc tcccttggtc
     1321 tctataccga gggagagtgg cgtgacgtcg aggagcagca cgtccttgac ctctcctgca
     1381 agcactccgg cctggatggc cgccccgacc gcgacgacct cgtcggggtt gatgcccttg
     1441 tggggctctt tgcctgtgat ctcctttacc tgctcctgaa ccgcggggat gcgggtcgag
     1501 ccgccgacga ggataacctg gtcgacctgg ccaggctgca gaccggcgtc ctccatcgcc
     1561 tggcgaactg gaccggccgt agcctcgacg aggtcagcgg tcaggttgtt gaactgcgcc
     1621 cttgtaagcg tcaggtccat atgcttcgga ccgttctggt ctgccgtaat gaaaggcagg
     1681 tttatcgagg tacttgtggt cgacgagagc tccctcttgg ccttctcggc tgcctcgacc
     1741 aggcgctggg cggccatctt gtccttcgag agatctatac cctctgcctt cttgaactct
     1801 gagaccatcc actcgacgac cttggcgtcg aagtcgtcgc cgccgaggtg gttgttgccg
     1861 ctcgtagcct tgacctcgaa gaccccctcg ccgagctcca ggatggagac gtcgaaagta
     1921 ccgccaccca aatcgaagac gagtatggtc tggtcctcct ccttgtccat accgtaagcc
     1981 agcgacgccg cggtcggctc attgataata cgcttgacgt tcaacccggc gatcctgcca
     2041 gcgtccttgg tcgcctggcg ctgggcatcc tcgaagtacg cgggcaccgt gatgacggcc
     2101 tcgctgacct cctcgccaag gtactcctcg gcgtccctct tgagcttctg gagggtcatc
     2161 gcggagatct cgggcggaga gtactccttg tcgtcgacct ctatccgaat gtccccgcca
     2221 gggcccgact tgacctggta gcctacgcgc tcggcctcag tcttcacgtc actgaagcgc
     2281 cgccccataa agcgcttgat cgaatagacc gtgcgctcag ggttcgtgac cgcctgcctc
     2341 ctcgccagct gccctaccag ccgctcgcca ctcctcctgt cgaaagccac gaccgaaggc
     2401 gtcgtccgct ctccttcgga gttggtgatc accgtaggat ccccaccctc cagtaccgca
     2461 acacaactgt tggtcgtgcc caaatctatg ccaatgctct tggccatgcg catacctccc
     2521 acgagataac gttatctaaa gtctcgtcag tataaaatct tagtgtcacg cttgcaagtt
     2581 tttcgtcgcc ggttgttgtt acggaggagg actggtatag ggtaggatag tgtggtagcc
     2641 ggtgtcgtgg acggcggtgg taggtttgag ggtgtaggga gttagattgg acgaaacgat
     2701 cctgttggtc gatgatgacg cggcgctctt ggaggtcacg tccatcatac tgacatctga
     2761 gggttaccgg gtagtgacgg ctgaggacgg gcccgaggcg ctccggatcc tcgggcgaga
     2821 gggtttggat ctcgtggtgc tcgacattat gctgccgagg atgagcgggt tcgaggtgtt
     2881 gaagaagatg cgcgagcaca gcgacgtccc tgtcgtcttg ctcacggcga agagccagtc
     2941 tgtagacaag gtagtcggac tagagctcgg ggcagacgat tacatcacca agcctttcga
     3001 caccaaagag ttgctggcca ggatcaaagc catcctgagg cgtttcggcc gggataacag
     3061 gggcagggag gggttattga agctcggctc tttggagttg gatcccaacg gctacaccgt
     3121 cagccgctcg ggggagatgt tggatctcac gcctaccgag ttcaagatac tcgcgctcct
     3181 catgcgccgt cccgggcagg cctttacgcg ggcccagatc tccgaggccg tctccacggg
     3241 gtcgcattac ctggcaagcc gctacataga cgtccacata agcaggctca gggccaaggt
     3301 cgaacgggac ccttcgagtc cggagatcgt acagaccgtc cctagcgtcg gatacagggc
     3361 agcgaggccc gtctaaaaga cggaggatgg gcatagaaaa gagag
//
LOCUS       DASTUV010000431         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1334359, whole genome shotgun sequence.
ACCESSION   DASTUV010000431 DASTUV010000000
VERSION     DASTUV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334359"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..471
                     /locus_tag="VFT03_08705"
     CDS             214..471
                     /locus_tag="VFT03_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848265.1"
                     /translation="MAYLEGQLRGNVADESEGEHTGYVLEVEVDMSAVQNAQDILGEQ
                     VAVTGEVEIVDYPERGKVLIFRATSAAAIEEEIEEEIEEVE"
     gene            complement(474..1304)
                     /locus_tag="VFT03_08710"
     CDS             complement(474..1304)
                     /locus_tag="VFT03_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU4848266.1"
                     /translation="MRVVCAQYAVRDGDPDHNLERSLQFIRRAAAEGADLIVLPELAN
                     SGCDLDSREQAPGLADEIPGGPTVQAWKKETEASDIYVVGGLLERERDTLYNSAVLLG
                     PGVFGRYRKTHLWNEEKLVYEPGRELPVFDTPLGMIGMLVCYDAWFPEAARTLAMKGA
                     EILCVPSNAPDDWVPEHQRRGGLTMLDIHCIAAANANRVFVAAANRVGDGYLGRSCVV
                     DATGGVLALAGVAEEGLIHAGVELSRARREKQLTDLSHSFEDRNPAVYEAGDTAGRDG
                     "
     gene            complement(1309..2127)
                     /locus_tag="VFT03_08715"
     CDS             complement(1309..2127)
                     /locus_tag="VFT03_08715"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU4848267.1"
                     /translation="MSGLSREAVEGLASPLAARVEAHYEVGSTQQRARELAREGAPHG
                     TLVLSGVQTGGRGRLGRRWGSPEGGLWMSLVLRPGISTRVAARVTQAAAVGVAKALRE
                     MGVRAEIKWPNDLLVDGRKICGILAESSVESVPAAAKKVGVGEERHVDFVVLGVGLNA
                     NLDPKDLGVPHNEAATLRSELGGDVDLLALLDSILSHLDFELGRIEDFGTVLDDWRAL
                     NCTLGERVRVRRFGEVLEGEALDLGPEGALLLETSDGMIELFEGEVEQLRRQGT"
     gene            complement(2124..3185)
                     /gene="cobT"
                     /locus_tag="VFT03_08720"
     CDS             complement(2124..3185)
                     /gene="cobT"
                     /locus_tag="VFT03_08720"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEU4848268.1"
                     /translation="MALEDRVRELAEEVEPADASAAQRARDLHRRLLKPPGSLGRLED
                     LGVRLAGMAGESPPPVPENPAVVVCAGDHGVLARGVSPWPKEVTAAMVENFCAGGAAV
                     NAIARTVGARVSVLDVGVASELERHPLLRGAKVRPGTADLSEGVAMSREDAARAVLAG
                     AGVAGELVESGGVDLLVTGDMGIGNTTPAACLVAAFTGSPAREVTGRGTGVDDATLEL
                     KTGLVEKALELHGPDPEDPVGVLAALGGLEHAALVGVILMGAVYGVPILLDGLVSNSA
                     ALVALSLAPRSVDYLVAGHISVEPGARVALEKLGLEPLLDLEMRLGEGTGALLAVPLV
                     QAASTVMREMETLEEAGIA"
     gene            complement(3188..4096)
                     /locus_tag="VFT03_08725"
     CDS             complement(3188..4096)
                     /locus_tag="VFT03_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEU4848269.1"
                     /translation="MRDLRPVANGKRRTSSEPALLIVGHGSRDPRGAEEFQELVSLVR
                     ARAPMPVEGGFIELSRPPISECVNNLRERGAREVSAVPLMLLAAGHAKDDVPATLVRE
                     KLTHPDLDFHYGRALGIRPELLELMDERISAVVPGEEREGTAVLIVGRGSSDPDANSD
                     LAKMARLFFEGRPYPLVETAFVSLAPPSVTEALERCKRLGAGHVAVFSYFLFTGVLEE
                     RIREQSRNFAEDNPEVEVRYAGYFGPDARVAALVMERYRESFEGDIRMNCDVCVHRVA
                     LPGFEEKVGAPATPHYHPDEHGHHHH"
     gene            complement(4071..4691)
                     /locus_tag="VFT03_08730"
     CDS             complement(4071..4691)
                     /locus_tag="VFT03_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEU4848270.1"
                     /translation="MRRVHPIEAESYRILREQVDLSHLGPLSRAVAERVIHASADLEY
                     AESLVLDEAALRRGFEALRRGARVVTDAGMVAAGITARKALCLVSDPRARLLAEHLDI
                     TRSAAGFRLAESEAGFDAVWVVGNAPTALFELLEMDVRPALVVGLPVGFVGAAESKER
                     LIRSGLPAVANHGPKGGSAVAAAALNALLYFEEDEEERIERSSPGG"
     gene            complement(4688..4843)
                     /locus_tag="VFT03_08735"
     CDS             complement(4688..4843)
                     /locus_tag="VFT03_08735"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU4848271.1"
                     /translation="MRTCPEKAIQPAPRGYPSPLTVFEDRCTGCAECAEVCPVAACVE
                     VIISERE"
BASE COUNT          772 a         1708 c         1596 g          774 t
ORIGIN      
        1 gcaccacctg gtcgacgtca tgtcctgagc gctctccggg agacctctgc tcgtcccctc
       61 catgctcagg agtcctatta ctcgaaccgg atcagccaca cctcctgctc tgacagccct
      121 gattccgagg tgtgtgttgg acatcccccg agcttgcggc cggacttccg gtaacatggc
      181 ccgcgtagga tgaactcgaa aaggaggtgc agcatggcat atctggaagg acagcttcga
      241 gggaacgttg ccgacgagtc agaaggcgag cacaccggct acgtgctcga ggtcgaggta
      301 gacatgtctg cggtccagaa cgcgcaggat atcctgggcg agcaggtggc cgtgaccgga
      361 gaggtcgaga tcgtagacta tcccgagcga gggaaggtct tgatcttcag agcgacttcg
      421 gcggcggcga tagaggaaga gatcgaagaa gagatcgaag aggtagagta gcctcagccg
      481 tcccggccag cggtatctcc ggcttcgtag accgccgggt tccggtcctc gaacgagtgg
      541 gagaggtctg tcagctgctt ctcgcgccgg gctctactca gctctacccc ggcgtggatc
      601 aaaccctcct ctgccacgcc agcgagcgcg aggacgccgc ctgtggcgtc taccacgcag
      661 ctacgtccca ggtagccgtc cccgacgcga ttggcggcgg cgacgaagac gcggttggcg
      721 ttggcagctg cgatgcagtg gatgtcgagc atcgtaaggc caccccggcg ctggtgctcg
      781 gggacccagt cgtcgggggc gttggacggg acgcagagga tctcggcgcc cttcatagcg
      841 agtgtgcggg ccgcctcggg gaaccaggcg tcgtagcaga cgagcatccc gatcatcccc
      901 agcggcgtgt cgaagaccgg aagctcccgg cccggctcgt acactagctt ctcctcgttc
      961 cagaggtgcg tcttgcggta gcgtccgaac accccgggtc ccagcaggac ggcggagttg
     1021 tagagggtat ccctctccct ctccaacagc ccgccgacga cgtatatgtc ggacgcttcc
     1081 gtctccttct tccacgcctg gacggtaggg ccgccgggga tctcgtccgc gagacctgga
     1141 gcctgttcgc gcgagtcgag gtcgcagccg gagttcgcga gctcagggag gacgatgagg
     1201 tccgctcctt cggcggcagc cctccggatg aactggaggg agcgctcgag gttgtgatcc
     1261 ggatccccat cgcggacggc gtactgggcg cagacaacgc gcatattctc aggtcccctg
     1321 gcggcggagc tgttcgacct cgccttcgaa caactcgatc atgccatcgc ttgtttcgag
     1381 gagcagggct ccctcggggc cgagatccag agcctcccct tcgagaacct ccccgaacct
     1441 ccggacgcgc acccgctcac ccagggtgca gttgagcgcc cgccagtcgt cgagtacggt
     1501 cccgaagtcc tctatcctac ccagctcgaa gtccaggtgc gagagaatgg agtcgaggag
     1561 cgccagaagg tccacgtcgc ctccgagctc ggagcgtaaa gtcgcggcct cgttgtgcgg
     1621 gacaccgagg tctttggggt cgaggttggc gttgaggccg acaccgagga cgacgaagtc
     1681 tacgtgtcgc tcctctccta ccccgacctt cttcgccgcc gcggggacgc tctctacgct
     1741 ggactcggcg aggatgccgc agatcttgcg cccgtccacg agcaggtcgt tgggccattt
     1801 gatctccgcc ctcactccca tctcccgcag agccttcgcc acccccaccg cagccgcctg
     1861 cgtaacgcgg gccgctaccc gggtggagat cccgggccgc agcaccagcg acatccagag
     1921 ccccccctcc ggcgagcccc acctccgccc gagacgcccc ctcccaccag tctgcacgcc
     1981 ggaaaggaca agcgtcccgt gcggggctcc ctcacgagcc agttcgcggg cgcgctgctg
     2041 cgttgagccc acctcatagt gcgcctcgac gcgcgccgca agcgggctcg ccaggccctc
     2101 gaccgcctcc cggctcaacc cgctcacgct atccccgctt cttcgagggt ctccatctcg
     2161 cgcataacag tgcttgcggc ctgcacgagc ggcacggcga gcagggcgcc ggtcccttcg
     2221 ccgaggcgca tctccaggtc gaggagaggc tcgaggccta gcttctccag cgcgaccctg
     2281 gcgcccggct cgaccgagat gtgccctgct accaggtagt caaccgaacg aggcgcgagg
     2341 ctcagggcga cgagcgccgc cgagttcgag acgaggccgt cgaggagaat agggacgccg
     2401 tagacggcgc ccatgaggat cacgccgacg agcgcagcgt gctccaggcc gccgagcgcg
     2461 gcgagcacgc ccaccgggtc ctcagggtcc gggccgtgca actccagcgc cttctccacc
     2521 aaccccgtct tcagctcgag cgtcgcgtcg tcaacgcctg tgccgcgtcc cgtcacctcc
     2581 ctggccggac ttccggtgaa ggcggcgacg aggcaggctg caggggtggt gttcccgatc
     2641 cccatgtccc ctgtaacgag caggtccacg cccccgctct cgacgagctc cccggcgacg
     2701 cccgctcccg cgagcaccgc gcgcgcggca tcctcccggc tcatcgccac gccctcactc
     2761 aggtcggcgg tccccggcct caccttcgcg ccgcgcagga gcgggtgacg ttcgagctcg
     2821 gaggcgacgc cgacgtcgag cacgctcacc cgcgcgccca cggtacgggc gatggcgttg
     2881 accgccgcgc ctccagcgca gaagttctcg accatcgccg ccgtcacctc tttcggccac
     2941 ggcgagacac cgcgggcgag cacgccatgg tctccggcgc agaccacgac cgcggggttc
     3001 tcgggcaccg gtggcgggga ctcgcccgcc atccccgcga ggcggacgcc gaggtcctcc
     3061 aggcgcccca ggctccccgg cggcttgagg agacgccggt gcaggtcgcg ggctctctgc
     3121 gccgcggacg cgtcggctgg ctcgacttcc tcggccagct cccttacgcg gtcctcgagc
     3181 gccacggcta gtggtgatgg tggccgtgct cgtcggggtg gtagtgaggt gtggcggggg
     3241 cgccgacctt ctcctcgaag ccggggaggg cgacgcggtg gacgcagacg tcgcagttca
     3301 tgcggatgtc gccctcgaag gactcgcggt agcgctccat gaccagggcc gccaccctcg
     3361 cgtcgggccc gaagtagccc gcgtagcgca cctccacttc ggggttgtct tcggcgaaat
     3421 tccggctctg ttccctgatg cgctcttcga ggacgccggt gaagaggaag taggagaata
     3481 cggcgacgtg cccggcgccg agccttttgc aacgctccag cgcctcggtc acgctcgggg
     3541 gagcaaggct cacgaaggcc gtctcgacga gcgggtaagg ccggccctcg aagaagagcc
     3601 gggccatctt cgcgaggtcg ctgttcgcgt cagggtcgga ggagcccctg cccactatga
     3661 ggaccgctgt cccttctctc tcctctccgg gaacgacggc ggagatgcgc tcgtccatga
     3721 gctccaggag ctcgggacgg atgccgaggg cgcggccgta gtggaagtcc aggtcggggt
     3781 gggtcaactt ctcgcgcacg agggtcgcgg ggacgtcgtc cttggcgtgg ccggcggcga
     3841 ggagcatgag cgggacggcg gagacctcac gcgcgcctct ctccctcaag ttgttcacgc
     3901 actcggagat tgggggccgc gagagctcta tgaatccgcc ctcgacgggc atcggggcgc
     3961 gggcgcgtac cagggagacg agctcttgga actcctctgc gccgcgcggg tcgcggctac
     4021 cgtgtccgac gatgagtagc gcgggctcgc tactcgtcct cctcttcccg ttagccaccg
     4081 ggcgaagatc tctcaattcg ttcctcctcg tcctcctcga agtacaagag ggcgttcagc
     4141 gccgcggccg cgaccgcgga gccccccttg gggccatggt tcgccaccgc cggcagcccg
     4201 ctccggatca agcgctcctt tgactcggcc gccccgacga acccgacagg cagcccgacg
     4261 acgagcgccg gcctgacatc catctccagc agctcgaaga gcgccgtcgg cgcgttcccc
     4321 acgacccaga ctgcatcgaa gcccgcctcg ctctccgcga gcctgaaccc cgccgccgag
     4381 cgcgttatgt caaggtgctc ggccagcagt cgcgcgcgag ggtcggagac taaacagagg
     4441 gccttgcgcg ccgtgatgcc cgcggcgacc atcccggcgt ccgtcacgac cctggcacct
     4501 ctgcgcaggg cttcgaaacc gcgcctcagg gccgcctcat cgaggaccag gctctcggcg
     4561 tactccaggt cggcagaggc gtggatcaca cgctcggcca ccgcgcgcga gagcggtccg
     4621 aggtgcgaga ggtccacctg ctcgcgcagg atgcggtagc tctcggcctc gatggggtga
     4681 acgcgtctca ttctctctcc gatattatga cctctacgca cgccgcgaca gggcagacct
     4741 ccgcgcactc ggcacagccc gtgcaacgat cctcgaagac ggtcaggggc gaaggataac
     4801 ccctgggggc gggttggatg gccttctccg ggcaagttct tatgcaggcc
//
LOCUS       DASTUV010000432         4627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1688629, whole genome shotgun sequence.
ACCESSION   DASTUV010000432 DASTUV010000000
VERSION     DASTUV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688629"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFT03_08740"
     CDS             <1..255
                     /locus_tag="VFT03_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848272.1"
                     /translation="FVFGFLAVGLLLICFAFGLAIIADVRGWGSFKVGSGVLTIISYR
                     RVPDGTQTVIGAGTIVLALLAGVLNAVAGAVLWARTPSRR"
     gene            complement(252..1481)
                     /locus_tag="VFT03_08745"
     CDS             complement(252..1481)
                     /locus_tag="VFT03_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4848273.1"
                     /translation="MNFDYSEKVQKLVSRLEAFMDELVYPNERVFEEQLEAAESRWTV
                     PPIMEELKDEAKAAGLWNLFLPESEYGAGLTNLEYAPLCEVMGRSPIAPEIFNCNAPD
                     TGNMEVLVRYGTPEQKERWLEPLLRGEIRSCFAMTEPDVASSDATNIRARIEREGDEY
                     VVNGRKWWSTGAGDPRCSLAIVMGKTDPSAPRHAQQSMILVPLDTPGVEIERTLPVFG
                     YDDAPHGHAEISFQDARVPASNMIWDEGKGFAIAQGRLGPGRIHHCMRLVGVAERALE
                     LMRERVREREAFGKPLAEQGVIMEWIADSRIEIEQARLLTLKAAHMMDTAGNKEARAE
                     IAMIKVVAPNVALRVLDWAIQAHGGAGVSDDFPLAYFWASARTLRLADGPDEVHRASV
                     AKLELKKERRVVPPPSP"
     gene            complement(1691..2581)
                     /locus_tag="VFT03_08750"
     CDS             complement(1691..2581)
                     /locus_tag="VFT03_08750"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU4848274.1"
                     /translation="MTLHGVCTIALTPFTGEGDLDEESIDSLAGFYLNSGVHGVTILG
                     IMGEAHKLSDVERQAVTERYISAIGGRVPVVAGCSAVATRIVVERARAAEEAGAAAVM
                     VAPPNNQRSLDLVFEHYRRVAEAVSVPVVVQDEPVNTGVVMPAPFLARLVDEIEGCRH
                     VKLEEAPTTIKISALLNNAKTEVGVFGGLGGMYFYEELARGAAGIMTGFAYPHVLVET
                     YRRFVEGDKREAQEYFFRYLPLIRFEAQLGVGGVGIRKEILKLRGVISSSHVRFPAPA
                     LDEETLRELEDLVEVLGLAG"
     gene            2655..4052
                     /locus_tag="VFT03_08755"
     CDS             2655..4052
                     /locus_tag="VFT03_08755"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HEU4848275.1"
                     /translation="MEQILHLIEHYGYLVVFFGVMLESTGVPLPGETILLASGFLAQR
                     GHLDVGDAILFGILGAVAGDQLGYWVGREGGRPFVLRWGRYVRVTPERLSRAEAFFAR
                     HGGKAVFLARFFSGLRVFGALVAGISRMRWEAFTLYNALGGTVWATAVVLVGYLVSGS
                     IGLVERWLGRATLLLAVLVAVVVGMYLLYRWVVAHRALLARYGEAARAYPPVARLGAR
                     YKVQLSWLLRRLTPGQYLGLHLTLGLAAAAGCLWLFGGLAEDVLTNDPIVRYDRAIAA
                     YLHSLANPPLTTFFLIVTALGSIETVALVGVVVAVILARRGQWLYLWTWAAAVGGSAL
                     LNHLIKDLFARPRPSFEHPLLLETSYSFPSGHAMESFVVYGMLAYFAVLTLRSSRART
                     AVVIGVALLVILIGFSRMYLGVHYFSDVVAGYAAGGVWLSALITGAETIRRSKSRSNQ
                     ALDEPPRERLPRASG"
BASE COUNT          728 a         1503 c         1513 g          883 t
ORIGIN      
        1 ttcgtgttcg gtttcctggc ggtcggcctc ttgctcatct gcttcgcttt cgggcttgcc
       61 ataatcgcgg acgtccgggg atggggttcc ttcaaagtcg ggagcggtgt gttgacgatc
      121 atcagctaca ggcgcgtgcc ggacggcaca cagacggtca tcggcgccgg aaccatcgtg
      181 ctcgcgctgc tggcgggcgt tctgaacgcc gtcgccggcg cagtgctgtg ggcacggacg
      241 ccgagcaggc gttagggact aggcggcgga acgacgcgac gctccttctt cagctccagt
      301 ttggctacgg acgcgcggtg gacctcgtca gggccgtcgg cgaggcgaag ggtacgggcg
      361 ctggcccaga agtaggcgag ggggaagtcg tcggagacgc cggcgccgcc gtgcgcctgg
      421 atcgcccagt cgagcacgcg cagagcgacg ttaggagcga cgaccttgat catggcgatc
      481 tcggcgcgcg cttccttgtt cccggccgtg tccatcatgt gcgccgcttt cagcgtcaag
      541 agccttgcct gctctatctc gatgcgcgag tcggctatcc actccatgat cacgccctgc
      601 tccgcgagcg gcttgccgaa cgcctcccgc tccctgaccc gctcgcgcat cagctccagc
      661 gcccgctcgg cgacgccgac gaggcgcatg cagtggtgga tgcggcccgg tccgaggcgc
      721 ccctgcgcta tagcgaagcc cttgccttcg tcccagatca tgttcgatgc gggaacacgc
      781 gcgtcctgga aggagatctc cgcgtggccg tgcggggcgt cgtcgtagcc gaagacgggg
      841 agagtgcgct ctatctcgac gccaggcgtg tccaggggga ccaggatcat ggactgctgc
      901 gcgtggcgcg gcgccgacgg gtctgtcttt cccatgacta tggcgaggct gcaacgcggg
      961 tcccccgccc ccgtagacca ccacttgcgc ccgttcacca cgtactcgtc cccttctctc
     1021 tctatgcgcg ccctgatgtt ggtcgcgtcg gaggaggcca cgtccggctc ggtcatcgcg
     1081 aagcacgacc gtatctcacc tctcagcaag ggctcgagcc agcgctcctt ctgctccggg
     1141 gtcccgtagc gaaccaggac ctccatattg cccgtgtcgg gcgcgttgca gttgaagatc
     1201 tcgggagcta tggggctacg gcccatgacc tcgcacagag gcgcgtactc caggttggtg
     1261 aggcccgcgc cgtactctga ctcgggcagg aagaggttcc agagccccgc cgccttcgcc
     1321 tcatccttga gctcctccat gatcggggga accgtccagc ggctctccgc ggcctcgagc
     1381 tgctcctcga acaccctctc gttcgggtag acgagctcgt ccatgaacgc ctcgagccgc
     1441 gacacgagct tctggacctt ctcggagtag tcgaagttca acggtgccct cccggcgtag
     1501 cgaaaagcct ggtgtcccct tcccagagcc gaattctggc acggcaatcc ggcgctggca
     1561 agcggctcgt cttctcagcc cggcaagcgc cagaacgggg aggtgaggcc gagcctggca
     1621 aagagatctc ttgcctcctc aacggtcctt ctccgccatc cgggctcccg ccgatcggcg
     1681 ccgtcgcgcc tcagcccgcg agtccgagta cctcgacaag gtcctcgagt tcgcgcagcg
     1741 tctcctcatc gagggcgggc gctgggaagc ggacgtgcga ggaggagatc acgcctcgca
     1801 gcttgaggat ctccttgcgg atgccgactc cccccacgcc gagctgggcc tcgaagcgga
     1861 tcaggggcag gtagcggaag aagtactcct gggcctcgcg tttgtcgccc tcgacgaagc
     1921 gcctgtaggt ttcgacgagc acgtgcgggt aggcgaagcc tgtcattatg ccggcggcgc
     1981 cgcgcgcgag ctcctcgtag aagtacatcc cgccgaggcc cccgaagact cctacctcgg
     2041 tctttgcgtt gttcaggaga gcggagatct tgatagttgt gggcgcctct tcgagcttga
     2101 cgtggcggca gccctcgatc tcgtccacca gcctggcgag aaacggcgcg ggcatgacga
     2161 cgcccgtgtt gaccggctcg tcctggacga ccaccggcac ggagacggcc tcggccaccc
     2221 gcctgtagtg ctcgaagacg aggtccaggc ttctctggtt gtttggcggc gcgaccatga
     2281 cggctgccgc gcccgcctct tccgccgcgc gggccctctc cactactatc ctcgttgcta
     2341 cagccgagca gcccgcgacc accggcacgc gaccgccgat ggcggagatg tagcgttcgg
     2401 ttaccgcctg ccgctcgacg tccgagagct tgtgcgcttc gcccatgatc ccgagtatgg
     2461 tgacgccgtg gacgccggag ttcaggtaga acccggcgag cgagtcgatg ctctcctcgt
     2521 ccaggtcccc ctcgccggtg aagggggtga gcgctatggt gcatacgccg tgcagggtca
     2581 tgggaccagt atagcgagga gagggtcaaa gtttaccctc ccagactgct cggcagacgc
     2641 tacactcttc ggcgatggag cagatactcc acctgatcga acactacggc tacctggtcg
     2701 tgttcttcgg ggtgatgctg gagagcacgg gcgtaccgct ccccggcgag accatcctgc
     2761 ttgcctccgg cttcctggcg cagcggggcc acctcgacgt gggcgacgcc atcctgttcg
     2821 gcatcctcgg ggcggtcgca ggcgaccagc taggctactg ggtggggcga gaggggggac
     2881 gcccgttcgt gctgcgctgg gggcgctacg tccgggttac ccccgagcgc ctctcccgcg
     2941 ctgaggcatt cttcgcccgc cacgggggga aagccgtatt cctggcccgc ttcttctcgg
     3001 gcctccgggt attcggggct ctggtggcgg gcataagccg gatgcgctgg gaggccttca
     3061 ccctctacaa cgcactcggc gggaccgtct gggcgactgc cgtggtcctg gtcggctatc
     3121 tcgtgagcgg gagcatcggg ctcgtggagc ggtggctggg acgggccacg ctactgcttg
     3181 ccgttctcgt cgccgtggtg gtcggcatgt acctcctata ccgctgggtg gtcgcgcacc
     3241 gagcgctgct tgcacgctac ggggaagccg cccgcgccta cccgccggtg gcgcgactcg
     3301 gagcccgcta caaagtacag ctcagctggc tgcttcggcg cctcacgccg ggccagtacc
     3361 tggggctgca cctgacgctc ggcctggcgg cggccgcagg gtgcctgtgg ctgttcggag
     3421 ggctggccga ggacgtcctc accaacgacc ccatcgtgcg ctacgaccgg gcgatagccg
     3481 cttacctgca ctccctggcg aacccgcccc tgacgacgtt cttcctgatc gtcacggccc
     3541 taggctccat cgagacagta gccctcgtag gggtggtcgt cgcggtgatc ctcgcccggc
     3601 gggggcaatg gctttacctc tggacctggg ccgcagcggt gggcggcagc gcgttgctca
     3661 accatctgat caaggatctg ttcgcccggc cccgaccctc cttcgaacac ccattgctgc
     3721 tggagacctc ctactctttc ccgagcggcc acgcgatgga gtccttcgtc gtctacggga
     3781 tgctggccta tttcgccgtg ctgacgctac ggagctcgcg cgcgcggacc gccgtggtaa
     3841 tcggcgtagc gctcctggtg atcttgatcg gcttcagccg gatgtacctc ggcgtccact
     3901 acttcagcga cgtcgtggcg ggatacgctg ctgggggcgt gtggttgagc gccctcatca
     3961 cgggcgccga gacgatccgg cgaagcaagt cccgctctaa ccaggccctg gacgagcctc
     4021 cgagagagcg ccttccgcga gcttcgggat aaggagatgc tcgggcttag cggttctggc
     4081 cggcaaattg agaagaacgg gcgccgcctg ctcgatgctc acctccggca ctccgtcatg
     4141 caatacggtc ctatatttga atagttttcc gtacaaaccg gaactcatac gcaggttggc
     4201 tatctgtacg aaaagaggcc cggtgtccct ggtagggttt gctgtgtcta gggaaaagaa
     4261 agggaaagga gtcggacacc gggttattgt ggtccattat ggacccagag tgcaaaagcc
     4321 cgataacgat tcgatcaagt cggattacgg tttgggcaca gttgggtaac agaaagctcc
     4381 ccagccggac gatgagtcgc cgctcccaag ccaagtggta gtgggaactt tgacagatca
     4441 atgtgtacgc ctggtttgca cccgtggcct tctgaagtct tctagaatgg gttgcgagcc
     4501 aaatcactag ttcttctttg cagcactttg ctacgaaagg gcaactcaga attacgaagc
     4561 cttgaagatt cgcttctccg ccacggtggg cgaggaggcg gagatcgagg tggcgttggt
     4621 ctagggg
//
LOCUS       DASTUV010000433         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1533975, whole genome shotgun sequence.
ACCESSION   DASTUV010000433 DASTUV010000000
VERSION     DASTUV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533975"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFT03_08760"
     CDS             <1..579
                     /locus_tag="VFT03_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848276.1"
                     /translation="AMLVPAIGGVWTVGVGSANGDVGRSPLGPVLVVPLTLVGLGAGL
                     PLLFGALMRPLGAALSAAVGALVLVGYDLTLDDGRVPYTGVPFDVIPATLDIPDLVAW
                     CGRILEYIQQYHPAFPLLFLLWAAMALAVSLGELAGRWLVGLGVAAGGGVLGYALLVS
                     EGSQALTDAVISLGLAAIIYAVLRYLVARVRG"
     gene            586..1425
                     /locus_tag="VFT03_08765"
     CDS             586..1425
                     /locus_tag="VFT03_08765"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEU4848277.1"
                     /translation="MGILKNIEKRMESLVEGVFGRAFRRQIHPVEIAKALTKQMDEGR
                     MVSISRTYAPNDFTVHLSEEDVESIRAYRDALKDELIQYASTHAENKDYHLMTPPRVR
                     FETEDSLRFGEFGITAKLTGGDGPREKGAPQDTSGQTRIFRTEEASGAKDQGTSAISP
                     EEAQRHGLAREVVEIVTKDGSFVLEGRGPWTVGRSQENDIVIPDPNVSRKHARLSRAD
                     NGYVVEDLGSTNGTLLDGAPIDRERIESGDELTFGQSTARFVRRIDSPREGERDPSGT
                     GRR"
     gene            1430..1909
                     /locus_tag="VFT03_08770"
     CDS             1430..1909
                     /locus_tag="VFT03_08770"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU4848278.1"
                     /translation="MLDLQVPLLAAKALLLLLLFAFIYAVMRRGIGDLRRVPDDELFE
                     PGRAQDGRGAYAPRGSAESELIVEDSEVLAPETRFEIRDGATSIGRSAASDIVLKSDD
                     YASGSHAQLTRHGGLLYVEDLGSTNGTFVNGRKTVGATPLRHGDSVRVGSTTFRYEE"
     gene            1921..3120
                     /locus_tag="VFT03_08775"
     CDS             1921..3120
                     /locus_tag="VFT03_08775"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU4848279.1"
                     /translation="MLYLEPFGATHAGKVRANNEDALLVGEGRDETLFVVADGIGGFE
                     AGEVASRIAVDVLKELEPGASFEAAISEANRRILVAGRGDERLSGMGTTVVALRFGGT
                     RDEPTAQVAHVGDSRAYLLRGGSLRPVTEDHSLVAELVRSGDLTRDQAAEHPQKNLIT
                     RALGADEEVEVDTAVLPVEAGDRFLLCSDGLSDMIPETRIGEILAEAPGDPEKPARSL
                     VSAALDAGGADNVTVIVVDVKAETAPHEERSGDTHEMPPVARSGDQAATRAPAQRPSR
                     KVPKPQTEARSRPSRRTKAARRKRRRSRFGSFVRGLAAVLVVVVALTPAYLWGSSRYF
                     LSIDEGEVVAYRGLPYAPLGVELNEEWRRTDVEWWEVKTPYQDPIENHKLYTKGEIEQ
                     VLQDLGK"
     gene            3124..>4032
                     /locus_tag="VFT03_08780"
     CDS             3124..>4032
                     /locus_tag="VFT03_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU4848280.1"
                     /translation="MTQRATSPMILALLITSLGFATLIFAQKATSPPLIYGAYYAGTL
                     FVLYLGVRLLLPHSDVLLLPIVTLLTGIGLVMIFRLTYDIKGAENLATTQAIWILVGS
                     GAMFLIVLLFRNYQRLFDYKYLLALAAVVLIASTFTPFGYEVNGARLWVAIGRVNFQP
                     SEFARIALVIFFAGYLAEKRDVMTATSRTFLRIQIPALKYFGPVALVWAASLGLLVFE
                     KDLGSSLLFFAVPLLMLYTATGRIAYVLLGGILFSIGSLMAYFLFGHVQLRVQSWLDP
                     WKDPDGTGFQILQSIFNIADGGMTGTG"
BASE COUNT          670 a         1319 c         1366 g          677 t
ORIGIN      
        1 gcaatgctcg tgccggctat cgggggggtc tggaccgtgg gcgtcggctc cgccaacggg
       61 gacgtggggc gctctcctct gggacccgtg ctcgtcgtgc cgctcaccct cgttgggctg
      121 ggggcggggc tgccgctgct cttcggcgcg ctcatgcgtc ctctgggagc ggcgctctcg
      181 gcggctgtcg gggcgctggt cctcgtcggc tacgacctca cgctcgacga cgggcgggtc
      241 ccctacaccg gcgtcccgtt cgacgtgatc cccgccacac tcgacatccc ggacctcgtg
      301 gcgtggtgcg gacggatcct ggagtacatc cagcaatacc accctgcctt tccgctcctg
      361 ttcttgcttt gggcggcgat ggcgctcgcg gtctcgctcg gggagttggc ggggcggtgg
      421 ctcgtcgggc tcggcgtggc agcaggaggc ggggtcctcg gatacgcgct gctggtctcc
      481 gagggttcgc aggcgttgac cgatgctgtg atttcgcttg gtctcgcggc tataatatat
      541 gcggtgttgc gatatcttgt tgcgagggtg agagggtagg gttctttggg catcctcaaa
      601 aacatcgaga agcggatgga gtcgctcgtc gagggcgtct tcgggcgtgc attcaggcgg
      661 cagatccatc ccgtagagat cgccaaggcg ctcaccaagc agatggacga gggccggatg
      721 gtcagcatct cgcgtaccta cgcccctaac gacttcaccg ttcacctctc cgaggaggac
      781 gtggagtcga tccgggccta ccgggacgct ttgaaggacg agctgatcca gtacgcctcc
      841 acccacgcgg agaacaagga ctaccacctg atgaccccgc cgagggttcg gttcgagaca
      901 gaggactcgc tgcgtttcgg cgagttcggg ataaccgcca agctgacagg cggggacggc
      961 ccgcgcgaga agggggctcc gcaggacacc tcgggccaga cccgcatctt ccgaaccgag
     1021 gaggcgagcg gggctaagga ccagggcacg tcagccatct ctcccgagga ggcccagagg
     1081 cacgggctcg cgcgggaagt ggttgagata gtgacgaagg acgggagctt cgtgctcgag
     1141 gggcgcggac cgtggaccgt cggccgctcg caagagaacg acatcgtcat ccccgacccg
     1201 aacgtctcca ggaagcacgc gcggctctcg cgcgccgaca acgggtacgt cgtggaggat
     1261 ctcggctcga ccaacggcac cctgctcgac ggcgcgccca tagaccgcga gcggatagag
     1321 agcggcgacg agctgacctt cggccagagc acggcccggt tcgtccgccg gatcgactcc
     1381 cccagagaag gcgagaggga cccttcgggc accgggaggc gttaaggtcg tgctcgacct
     1441 ccaggtgccg cttctggcgg cgaaggcgct gctcttgctg ttgctcttcg cctttatcta
     1501 cgccgtgatg cgcaggggga tcggggacct ccggagggtc cccgacgacg agctcttcga
     1561 gcccggacgg gcgcaggacg ggcggggcgc ttacgcgccg cgcggctcgg ccgagtccga
     1621 gctcatcgtc gaggactccg aggtcctggc ccccgagacc aggttcgaga tccgggacgg
     1681 cgcgaccagc atcggccgct ctgcggctag cgacatcgtc ctcaagagcg acgactacgc
     1741 ctctgggagc cacgcccagc tcacccgcca cggagggctc ctgtacgtgg aggacctggg
     1801 ctcgacgaac ggtacgttcg tcaacgggcg caagaccgtc ggcgcaactc ccttgaggca
     1861 cggggacagc gtgcgggtcg gctcgacaac cttcaggtac gaggagtagt ctctagggct
     1921 atgctttacc tggaaccctt cggcgccacc cacgcgggaa aggtccgcgc gaacaacgaa
     1981 gacgcgctgc tcgtcggtga gggccgcgac gagactctgt tcgtggtcgc cgacggcatc
     2041 ggcggcttcg aagccgggga agtagcgagc cgcatcgccg tagacgtgct caaagagctc
     2101 gagcccggag cctccttcga ggccgccata agcgaggcaa accgccgcat cctcgtagcc
     2161 ggcaggggcg acgagcggct ctccgggatg gggacgaccg tcgtggcctt gcggttcggc
     2221 ggcacccgcg atgaaccgac ggcccaggtc gcgcacgtcg gagactcgcg ggcgtacctg
     2281 ctgcgcggcg ggagcttgag gcctgtgacg gaggaccatt cgctcgtcgc cgagctggtg
     2341 cgcagcgggg acctgaccag ggaccaggcc gcagaacatc cccagaagaa cctcatcacc
     2401 cgcgctcttg gcgcggacga ggaggtcgag gtggataccg cggtcctgcc agtggaggcg
     2461 ggggaccgct tcctcctgtg ctccgacggc ctctccgaca tgatcccgga gaccaggata
     2521 ggggagatcc tggccgaagc tccgggcgac cccgaaaagc cggcccgaag cctggtctcc
     2581 gctgccctcg acgccggcgg cgcggacaac gtgaccgtga tagtcgttga cgtaaaggcg
     2641 gaaaccgccc cgcacgagga gcgctccggc gatactcacg agatgccacc agtcgcccgg
     2701 tcgggggacc aggctgccac ccgagcgcca gcgcagcggc cctcgagaaa ggtgcccaag
     2761 ccccagacag aagccaggag ccgcccctcc cggcgaacca aggctgcgcg caggaagcgc
     2821 aggagaagcc gcttcggcag cttcgtgcgg ggtcttgcgg ccgtgctggt ggtcgtcgtc
     2881 gcgctgacgc cggcgtacct gtgggggtcg agccggtact tcctgagcat cgacgaaggc
     2941 gaggtggtgg cgtaccgggg attgccctac gcgcctctcg gcgtcgagct caacgaggag
     3001 tggcgcagga cagatgtcga gtggtgggag gtcaagacac cctaccagga tccgatagag
     3061 aaccacaagc tctacaccaa gggtgagata gagcaggttc tgcaagatct tgggaagtag
     3121 cggatgacgc agcgcgccac cagcccgatg atcctggccc tgctcataac ctctctgggg
     3181 ttcgcgacgc tgatcttcgc gcagaaagcg accagcccgc cgctgatata cggcgcctac
     3241 tacgcgggga cgctgttcgt gctctacctt ggcgtgaggc tcctcttgcc gcactcggat
     3301 gtactgctct taccgatagt gacgctgctc accggcatcg ggctcgtcat gatcttccgc
     3361 ctcacctacg acatcaaggg cgcggagaac ctggcgacga cccaggctat ctggatcctg
     3421 gtcggcagcg gggcgatgtt cctcatcgtg ctgctcttcc gcaactacca gcgcctcttc
     3481 gactacaagt acctcctcgc cctcgcggct gtcgtcctta tcgcctcgac gttcacgccc
     3541 ttcggatacg aggtcaacgg ggcgcggctc tgggttgcca tcggccgtgt caacttccaa
     3601 ccctccgagt tcgcccgcat cgcgctcgtt atcttcttcg cgggctacct ggcggagaag
     3661 cgcgacgtca tgaccgcgac gagccgcacc ttcctgcgga tacagatccc cgcgctcaag
     3721 tacttcgggc cggtagccct cgtgtgggca gcctctctag ggctcctggt cttcgagaag
     3781 gacctcggga gcagcctcct cttcttcgcc gtgccgctct tgatgctcta cacggcgacg
     3841 ggccgcatcg cctacgtcct gctcggcggg atcctcttct ccatcggctc gctcatggcg
     3901 tacttcctct tcggccacgt gcagctgcgg gtgcagagct ggctcgatcc ctggaaggac
     3961 cctgatggga cgggattcca gatcctccag agcatcttca acatcgcgga cggcgggatg
     4021 accggcacgg gc
//
LOCUS       DASTUV010000434         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1365166, whole genome shotgun sequence.
ACCESSION   DASTUV010000434 DASTUV010000000
VERSION     DASTUV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365166"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFT03_08785"
     CDS             complement(<1..556)
                     /locus_tag="VFT03_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4848281.1"
                     /translation="MADTRTNARQEEESKPGLRRLLPRLAGSAFIFLILAGLMIAFSI
                     LEGTAFLSGENFLNIAVDASELLLLAVGVTFVIITAGIDLSVGSVLVLSSVIAAQTMV
                     ALSGTDQQVQNYTYPNQAVGIPVGIAAGLLTGLVCGLINGLLVTKLKLTPFIATLGTL
                     GVFLGTAQILSGGTNVPYVPPAIQI"
     gene            complement(559..1329)
                     /locus_tag="VFT03_08790"
     CDS             complement(559..1329)
                     /locus_tag="VFT03_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848282.1"
                     /translation="MRDNQVVLQVRGINKSFGAVQALKHVDFEVRSGEVTALIGDNGA
                     GKSTLINVITGVLAPDSGEIIFEGEKVEFASPHDARERGIETVYQDLAVAPQLDAVAN
                     IFLGRERRMTGLLGRLGFLDQQRMQRETGEQLEDLHVRIPDPTKPLSTFSGGQRQGVA
                     VARAVMWASKVVIMDEPTAALGVAQVGMVLDLVRQVRQTGIPVVFISHNMPQVFDVAD
                     RIVVLRLGELVAELDPERASTDDAVAAMTGSLRGEERG"
     gene            1577..>2281
                     /locus_tag="VFT03_08795"
     CDS             1577..>2281
                     /locus_tag="VFT03_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4848283.1"
                     /translation="MSQDVPLQNRVIDKLIECIRDAVVVAEAHTGRIVEWNAAATRIF
                     GYSSSEVLGHSWTVIVPERLKAQCEAGMARYRDTGHGPYIDSHAVLELPAVRKDGEEI
                     TIEIVLSPMEPIRDLGETEGRLVFAIIRDVTEREEVKKDLRASEERYRSLVQNTSDII
                     TILDADGTIIYISPAAERMMGYKPEERVGTNAFDSVHPDDRERASGIFAEILKTPGSH
                     LPLEFRVQHRDGSWRYL"
BASE COUNT          447 a          728 c          710 g          396 t
ORIGIN      
        1 gaatctgtat cgctggcggg acatagggga cgttcgtgcc cccggagagg atctgcgccg
       61 tccccaggaa taccccgagg gtgccgagcg tggcgatgaa gggcgtcagc ttgagcttgg
      121 tgacgagcag cccgttgatg agcccgcaga ccagcccggt caggaggccg gccgcgatcc
      181 ctacgggtat gcccacggcc tggttcgggt aggtgtagtt ctggacctgc tggtccgtgc
      241 cggagagcgc caccatggtc tgggcggcga tcacgctgga gagcaccagc accgacccga
      301 ccgacaggtc gatgccggcg gtgatgatca cgaacgtgac gcctactgcc agtagcaaga
      361 gctccgaggc gtcaacggcg atgttgagga agttttcccc gctaaggaag gccgtccctt
      421 ccagaatgga aaaggcgatc atcagccccg cgagtatgag gaagatgaag gctgaaccgg
      481 caagacgggg gagtaaccgg cgaagccccg gtttggattc ctcttcctgc cgggcattgg
      541 tccgagtgtc ggccacgcct atcctctctc ctcaccgcgc agagatccgg tcatggccgc
      601 caccgcatcg tccgttgagg ccctctcggg atcgagctcc gcgaccaact ctccaagccg
      661 caaaacgact atgcggtcgg cgacgtcgaa gacttgcggc atgttgtggc taatgaacac
      721 gaccggtatg ccggtctggc gtacctgcct cacgaggtcg agcaccatcc cgacctgggc
      781 aacgcccaga gcagctgtgg gttcgtccat gatgaccacc ttgctggccc acatcaccgc
      841 ccgggccacc gctaccccct ggcgctgccc gccggagaaa gtgctcaaag gcttggtcgg
      901 gtctgggatc cgcacgtgca ggtcctccag ctgctcgccg gtctctctct gcatcctttg
      961 ctgatccaga aacccgagcc ggcccagcag ccccgtcatg cggcgctccc tgccgagaaa
     1021 gatgttcgcc accgcgtcca gctgtggcgc caccgcgagg tcctggtaca cggtctcgat
     1081 cccacgctcg cgggcgtcgt gcggtgaagc gaactccacc ttctccccct cgaagatgat
     1141 ctcgcccgag tcgggcgcga gcaccccggt tatgacgttg atcagcgtag acttgccggc
     1201 tccgttgtcc ccgatgaggg ccgtaacctc gcccgaacgg acctcgaagt ctacgtgctt
     1261 gagggcctgc accgccccga aggatttgtt tatcccccgg acctgcagca ctacttggtt
     1321 atcacgcacc cgcctcacct cttcgccggt gtgatccaca cagcatagag caatccggcg
     1381 tctcgggcct tcgcaacgcc ttcaggagaa ccttgcattg ccgggttccc gccatgtggc
     1441 gcacaaagtg agtcgtggac accagattgc gagcctcggg aaccttttgt aagctagcgc
     1501 aagagtacta ctcagagatg gagggccaca gagcgatacg cgaagaacgc caacgagcgt
     1561 ccgccgtgta tgtggagtga gtcaggacgt gccgctacag aaccgggtca ttgacaagct
     1621 aatcgagtgc atccgggatg cggtggtcgt cgcggaggcg cataccggaa ggatcgtgga
     1681 gtggaacgcg gccgcgaccc ggatcttcgg atactcctca tcagaggtgc tcgggcacag
     1741 ttggaccgtg atcgtccccg aacgcctcaa ggcgcaatgc gaggcgggca tggcccgcta
     1801 ccgggatacg ggccacggac cctacatcga ctcgcacgcc gtgctggagc tacccgcggt
     1861 gcgcaaggac ggagaggaga tcaccatcga gatcgtcctc agccccatgg agccgatccg
     1921 cgatctgggg gagaccgaag ggcgcctggt gttcgccatc attcgcgacg tcaccgagcg
     1981 tgaggaggtc aagaaggatc tcagagcgag cgaggagcga taccgctctc tggtacagaa
     2041 cacctccgat atcatcacga tcctcgacgc cgatggcact atcatctaca taagcccggc
     2101 ggccgaaagg atgatgggct acaagcccga agagcgggta gggaccaacg catttgactc
     2161 cgtccatcct gatgacaggg agcgtgcgtc gggcatattc gccgagatcc tgaaaactcc
     2221 tggcagtcac ctacccctgg agttccgggt gcagcacagg gacggctcct ggcgctacct
     2281 c
//
LOCUS       DASTUV010000435         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1445700, whole genome shotgun sequence.
ACCESSION   DASTUV010000435 DASTUV010000000
VERSION     DASTUV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445700"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFT03_08800"
     CDS             <1..435
                     /locus_tag="VFT03_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU4848284.1"
                     /translation="SDIILQHVQPEDLLRYGLIPEFVGRLPVISTLRSLEQEDLVRIL
                     TEPKNALVRQYRQFFRYDKVDLSFTDEALSAIAELAMERGTGARGLRSILETALLPTM
                     YELPSRSDVKGCVVDADTVRDGVQPSLVTGSSKYSYDEEETA"
     gene            442..>2697
                     /locus_tag="VFT03_08805"
     CDS             442..>2697
                     /locus_tag="VFT03_08805"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU4848285.1"
                     /translation="MSQAELPKTYDPHAVEGRIYAEWERRGVFEADTSPTKDPYSIVL
                     PPPNVTGKLHMGHALNGAIQDALTRRARMKGYEALWLPGVDHASIALQNVVERKIMRE
                     EGKTRWDLGREEFVERCRRFAEEARSQILEQLKRLGASADWRRTRYTMDEEYIDSVLT
                     AFIELYNAGLIYRGSRITNWCPFDRSAISDLEVNYEETDGKLYGLRYPFVDGRGPGPD
                     GNEYAQVFSTRPETMLGDVALVVNPDDERYTGLIGRRVMVPFVEREIEVFADEYVDPS
                     FGTGVLKVTPAHDPNDFEIAGRLGLDPVNILNPDGTINEVGGPFAGMSREDARAAVVA
                     RLDEIGLLGEVRDYRYRVGHCDRCGTVIEPWLTEQWWVSMRELAEPAIEVLRKREIVV
                     YPDSWRRETIRWLENIHDWNVSRQLWWGHRVPVWYGPNGEVVASKESPGEGYEQDPDV
                     LDTWFSSALWPFATLGWPEETEDLGYFYPTSLLSTAREIMYLWVARMIMMGLRFMDDV
                     PFEKVNVHSIVLAEDGTKMSKSKGNTIDPLDLIDEYGADAVRFGLLYQSSTQDFAYSY
                     ERAELGRSFVTKLWNATRFVLGYTRVEEPREASPSDRWILSEFNRTVREYDHLLEDCE
                     FSEAMRVIYSFAWNQFADWYIEIAKAAPSPATPHVLREVFSGVLKLLHPVMPFATEEM
                     SHILGSDELLARHMFPEYDSSLEDEEAERLLDRTKRAVSAVRSFRAEAKVEGELKGRV
                     SGEVDLEVFSAL"
BASE COUNT          501 a          896 c          849 g          451 t
ORIGIN      
        1 tccgacatca tcctgcagca cgtgcagccc gaggacctcc tgaggtacgg gctcatcccc
       61 gagttcgtgg gacggctgcc ggtgatctcg accctccgga gcctggagca ggaggacctg
      121 gtgcgcatcc tcaccgagcc caagaacgcc cttgtgaggc agtaccggca gttcttccgc
      181 tacgacaagg tagacctctc ctttaccgac gaggcgctca gcgcgatagc cgagctcgcg
      241 atggagcggg ggacgggtgc gagggggcta cgcagcatcc tggagacggc gctcttgccg
      301 actatgtacg agctgccgag ccgctcggac gtgaaggggt gtgtagtaga cgccgacacg
      361 gtcagggatg gcgtccagcc ctccctcgtg acaggctcct ccaaatactc ctacgacgaa
      421 gaagagaccg cctaaaaacc tttgagccag gccgagctac ccaaaaccta cgatccccac
      481 gccgtcgaag gccgtatcta cgccgagtgg gagcgccgcg gcgtattcga ggccgacacc
      541 agccccacca aggaccccta ctccatagtc ctgcccccgc ccaacgtgac agggaagctg
      601 cacatggggc acgccctgaa cggtgcgatc caggacgccc tcacgcggcg ggccaggatg
      661 aagggctacg aggcgctgtg gttgcccggt gtggatcacg cctccatagc gctccagaac
      721 gtcgtggagc gcaagatcat gcgcgaggaa gggaagaccc gctgggacct cgggcgcgag
      781 gagttcgtcg aacgctgccg gcgcttcgcc gaggaggcgc gctcccagat actcgaacag
      841 ctcaagcggc taggggcttc cgccgactgg cggcgcacgc gctacacgat ggacgaggag
      901 tacatagact cggtgctcac ggcgttcatc gagctgtata acgccggcct gatctaccgc
      961 ggcagccgta tcaccaactg gtgcccgttc gaccgctccg ccataagcga cctcgaggtc
     1021 aactacgagg agacggacgg gaagctctac ggcctccgct accccttcgt tgacggccgt
     1081 ggccctgggc ccgacggcaa cgagtacgcc caggtcttct cgacaaggcc cgagaccatg
     1141 ctcggcgacg tcgccctcgt cgtcaacccg gatgatgagc ggtacaccgg cctcatcggg
     1201 cggagggtca tggtcccatt cgtcgagcgg gagatagagg tcttcgccga cgagtacgtc
     1261 gacccctctt tcggtaccgg cgtgctcaaa gtcaccccgg cgcacgaccc caacgacttc
     1321 gagatcgcgg gtcggctcgg cctcgaccct gtcaacatcc tcaacccgga cggcacgatc
     1381 aacgaggtgg gaggcccctt cgcggggatg agcagggagg atgcccgcgc cgccgtcgtg
     1441 gcgcggttgg acgagatcgg tctgctcggg gaggtccgcg actaccgcta cagggtcggg
     1501 cactgcgacc ggtgcgggac ggtcatcgag ccctggctca ccgagcagtg gtgggtctcc
     1561 atgcgcgagc tcgccgagcc cgccatagaa gtgctcagga agcgtgagat cgtcgtctac
     1621 ccggactcct ggcgccgcga gaccatacgc tggctcgaga acatccacga ctggaacgtc
     1681 tccaggcagc tctggtgggg ccaccgagtc cccgtctggt acgggccgaa cggcgaggtc
     1741 gtcgcctcca aggagtcgcc aggcgaaggt tacgagcagg accctgacgt gctcgacacc
     1801 tggttctcct ccgctctgtg gcccttcgcc actctgggat ggcctgaaga gaccgaagac
     1861 ctcgggtact tctaccctac gagcctcctc tcgacggcgc gtgagatcat gtacctctgg
     1921 gtcgcgcgca tgattatgat gggcctgcgt tttatggatg acgtcccctt cgagaaggtg
     1981 aacgtccact ccatcgtact cgccgaggac ggcacgaaga tgagcaagtc caagggcaac
     2041 acaatagacc cgctcgacct catcgacgag tacggcgctg acgccgttcg cttcgggttg
     2101 ctctaccagt cctccacaca ggacttcgcc tactcctacg agcgcgccga gctgggccgc
     2161 agcttcgtca ccaagctctg gaacgcgacg cgcttcgtcc tgggctacac gcgcgtcgag
     2221 gagccgcgcg aagcctcgcc gtcggaccgc tggatcctat ctgagttcaa ccgtaccgtc
     2281 cgcgagtacg accacctgct cgaagattgc gagttctccg aggcgatgcg cgtgatctac
     2341 tcctttgcgt ggaaccagtt cgcagactgg tacatcgaga tagcgaaggc cgccccctcg
     2401 ccggcgacgc cgcacgtcct tcgcgaggtc ttctccggcg tcctgaaact cctgcaccct
     2461 gtcatgccct tcgcgacaga agagatgtca cacatcctcg ggtcggacga gctgctcgct
     2521 cgccatatgt ttcctgaata cgactcctcg ctcgaagacg aggaggccga gcggctgctc
     2581 gatcgcacca agcgcgccgt ctcggccgtg cggtcgttcc gggccgaggc gaaggtcgag
     2641 ggtgaactga aaggacgcgt ctcgggggag gtggacctcg aagtcttctc ggcgctc
//
LOCUS       DASTUV010000436         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1548778, whole genome shotgun sequence.
ACCESSION   DASTUV010000436 DASTUV010000000
VERSION     DASTUV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548778"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..359)
                     /locus_tag="VFT03_08810"
     CDS             complement(165..359)
                     /locus_tag="VFT03_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4848286.1"
                     /translation="MLGIGGSEIVIIALLVLIIFGPSKLPQMARDLGRFVGEARRSID
                     EFKDELTASSDEDEEEEEKR"
     gene            446..1486
                     /locus_tag="VFT03_08815"
     CDS             446..1486
                     /locus_tag="VFT03_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU4848287.1"
                     /translation="MGDAPKMISWGAVRTIAVALASRTERASDGDFDYEWAVERAVDP
                     LSRFTGIELPQGPPSGRQLRVASRAEWIDFNIEGFGALMEPVLKRAAAGSDNLTWAIG
                     GATLTAQVGLLLGFLSARVLGQYDTGPLLARERAGEPGKVFFLDGNIAAAAGRIAVPV
                     DGLRLWIVLHEMTHALQFEGYPWLRAHLGELLERLISPLADRLGVRETIRRLSENMRT
                     GGRSVELMMNRSQRESFDQMQATMSVIEGYSDYVMHSVGRSLVPQYEHLKNRMARSRA
                     HRPPFETAVFRITGLDVKLEQYRLGERFAEAVATREGMEGLNRVWERPENLPTLEEVR
                     DPGIWMTRMEAS"
     gene            1488..1808
                     /locus_tag="VFT03_08820"
     CDS             1488..1808
                     /locus_tag="VFT03_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848288.1"
                     /translation="MEHPSLGYEFAREDEARGSAAELDRRFADRELKGLRIYRVRWNG
                     NYLVEAVFADDTTDSRLQDARALLGEAGVPVHPDDLEDYKKATDEGGAGLPDWIRRFF
                     GGRY"
     gene            complement(1827..2120)
                     /locus_tag="VFT03_08825"
     CDS             complement(1827..2120)
                     /locus_tag="VFT03_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848289.1"
                     /translation="MGEGTTKFFIGAAVGAVGGFAVGTFAATPTARSASQSALGGLDT
                     SIRFLGRTVIQAIDVLGSTLESGYTRVRGREKYLEHEIEELREQITRLEQRMD"
BASE COUNT          403 a          711 c          768 g          387 t
ORIGIN      
        1 gccggaagat tagcacgctc ctgcttcgcc gtcgcttgtc agcacgctgc agccttcggc
       61 ttcgccaggc gccgtgctga gaagccgacc gaagggaggc gtgctgacaa gcgacgccct
      121 aaaagggcgg agcgtgctga aatgctgccg aaacggcagc atttctagcg cttctcctct
      181 tcctcttcgt cttcgtcgga ggaggcggtc agctcgtcct tgaactcgtc tatcgagcgg
      241 cgggcctcgc ccacgaagcg gccgaggtcg cgtgccatct gagggagctt gctggggccg
      301 aagatgatca ggacgagcag agcgatgatg acgatctctg atccaccgat gccgagcatg
      361 gactaaatat aacccccgga ggtccggctt acaacgcgcg cagcgtcgcg gggtttgtag
      421 cgtatatact caagggcgtg acgacatggg cgatgcacct aaaatgatct cctggggcgc
      481 ggtccggacg atagccgtgg cgctcgcctc gcgtacggag agggcctcgg acggcgactt
      541 cgactacgag tgggcggtcg agcgggctgt agatcccctc agccgcttca ccggcatcga
      601 gttgccgcag ggcccgccga gcggacgcca gctgcgggta gccagcaggg cggagtggat
      661 agacttcaac atcgagggct tcggagcgct catggagccg gtcctgaagc gcgccgccgc
      721 tggctcggat aacctgacct gggccatcgg gggcgcgacg ctcacggcgc aggtcgggct
      781 tctcctcgga ttcctctcgg cccgggtcct cgggcagtac gatacggggc cgctgctcgc
      841 gcgagagagg gccggcgagc ccggcaaggt gttctttctg gatgggaaca tagccgcggc
      901 ggcgggcagg atcgcggtgc ccgtggacgg tctcaggctc tggatcgtgc tgcacgagat
      961 gacccacgcc ttgcagttcg agggttatcc ctggctgcgc gctcacctcg gcgagctcct
     1021 ggagaggctg atctccccgc tcgccgaccg cctcggcgtc agggagacta tcaggcgcct
     1081 ctccgagaac atgaggaccg gcggccgctc ggtagagctg atgatgaacc ggtcccagcg
     1141 cgagagcttc gaccagatgc aggcgaccat gtcggtgatc gagggctact cggactacgt
     1201 catgcacagc gttggcaggt cccttgttcc acaatacgag cacctcaaga accgcatggc
     1261 gaggagccgg gcccaccgcc cgcctttcga gaccgccgtg ttccgcataa cgggcctgga
     1321 cgtgaagctc gagcagtacc ggctcgggga gcgcttcgcc gaagccgtcg ccacaagaga
     1381 gggcatggag ggcctgaacc gcgtctggga gcgtcccgag aacctgccga ccctcgaaga
     1441 ggtcagggac cccgggatct ggatgaccag gatggaggcc tcctgaggtg gagcatcctt
     1501 ctctgggcta cgagttcgct cgggaagacg aggccagggg ctccgccgct gagctcgacc
     1561 ggcgcttcgc cgatcgggag ttaaagggtc tgcgcattta cagggtgcgc tggaacggca
     1621 actacctcgt cgaggccgtc ttcgcggacg acacgactga ttcgcgcctg caagacgcca
     1681 gggcactgct cggtgaggcg ggagtcccgg tgcatcccga cgacctcgaa gactacaaga
     1741 aggcgacgga tgagggtggc gccgggctgc cggactggat ccggcgcttc ttcggggggc
     1801 gttactagac tagcggtctg acgcgtctag tccatgcgct gctcgaggcg cgtgatctgc
     1861 tccctcagct cctctatctc gtgttcgagg tacttctccc tgcccctgac acgcgtgtag
     1921 ccgctctcca gggtagagcc gagaacgtct attgcctgta tcacggttct gcccaggaag
     1981 cggatcgagg tgtcgagccc gccgagggcc gactggcttg ccgagcgggc ggtgggggtc
     2041 gcggcgaagg tccccacggc aaagccgcct accgcaccga cggccgcccc tatgaagaac
     2101 ttggtagtcc cttcgcccaa ccgaaaatcc ctcccgcatc accgagcttc gacgcggcca
     2161 gtttagcaca gcgccccaac ggtccggctg cgcaagcgag aggaagcaag acgaagccct
     2221 ttcggacacc ttgcaagaac acgggcgcgt tatactgggt cgggtatgt
//
LOCUS       DASTUV010000437         6050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_858198, whole genome shotgun sequence.
ACCESSION   DASTUV010000437 DASTUV010000000
VERSION     DASTUV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858198"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFT03_08830"
     CDS             <1..618
                     /locus_tag="VFT03_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEU4848290.1"
                     /translation="TQDWPREYMRPNFFPNTHDILPHILQFGGRPAFQTRFVLAATLS
                     STYGIYSGFELCENTPRGEPGTTVYYQDSEVYEYKVWDWDRPGNIRDYIRRVNEIRRD
                     NPALHYLKNLSFYEAPNDNVLFYGKRTGDNNIFVAVNLDPDVAHDTHVQFPLWEFGIG
                     ETEGYVVEELITQQEMPATGSWFWVRLDPESNPAEIFRLRKAGVR"
     gene            615..3992
                     /gene="treS"
                     /locus_tag="VFT03_08835"
     CDS             615..3992
                     /gene="treS"
                     /locus_tag="VFT03_08835"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEU4848291.1"
                     /translation="MTEVSSRGARGFEEDPEDPLWYKDAIIYHLHVKAFFDSNNDGIG
                     DFKGLTQRLDYIKDLGVTAIWVMPFYPSPMRDDGYDISEYKNVHADYGTRRDFRAFVR
                     RAHELGLKVITELVINHTSDAHPWFQRARKAQKGSAKRNFYVWSDTPDRYEGTRVIFT
                     DTERSNWTWDEVAEQYYWHRFFSHQPDLNHDNPQVFKAIMGVMRFWLDAGVDGLRLDA
                     IPYLIEREGTDNENLPETHEVLKRMRAEMDARYHNRLFLAEANQWPEDVLPYFGEGDE
                     CHMAFHFPLMPRIYMALAQEDRHPIVEIMHQTPEIPEICQWAIFLRNHDELTLEMVTD
                     KERDYMYQTYATDPRMRVNVGIRRRLAPMMENDRPRIELLNSLLMSMPGTPIIYYGDE
                     IGMGDNIFLGDRDAVRTPMQWTSDRNAGFSRAEPARLYLPPIMDPVYGYESVNAEAQS
                     RSAGSLLNWMKRIISVRKAHKAFGRGTLEFLHPGNRKVLAYLREYEDESILCVANLSR
                     SAQPVELDLARFRGRVPVELLGNSSFPPLGDLPYFLTLPGYSFYWFRLATEAAAPSWH
                     QESPRYAEPPVLVLPAGRETARRVYGAPLRLPSRQLAQLEREALPNFLGTRRWFAAKG
                     GEIERVEVAAQGEFDHNGLLTLLRVHVDEGEGPQLYFLPLAAAWEEGADEDGVWALIP
                     HALAKLRRRARMGLLLDAMADESFCRHVVEAMGKNRDFGLGEGKVHFSATEVFGELAG
                     EEPLSELGVRRAEVEQTNSSVILGERLILKAYRRLQKGTNPDLEIGRYLTEEVRFENT
                     PPLAGAVEYQGPDGETTTLGLLQGFVESQGDGWSYVLDYLGRYLEDRLVTSWSLEGEE
                     GAEAEREERSAEDAFFASLMRTLGLRTGELHAAFAAPTYDTAFAPELAPPEEVAGWAE
                     AILAELKRTFDTLKRRRDRLPEEVVPDAYRLLGLRGELTRRVKDVSRGGFGVVKTRHH
                     GDYHLGQVLVVGNDFQIIDFEGEPARPLAERRRKHSPLRDVAGMLRSFDYAARSAVMN
                     LGAERAEQLESLEPWVRLWQERAREAFLEGYAEGARGAASYPDDEEHARTLVELFTLE
                     KALYEIRYELDNRPDWVGIPIGGILDLLGEPVGEEES"
     gene            3989..>6050
                     /gene="glgB"
                     /locus_tag="VFT03_08840"
     CDS             3989..>6050
                     /gene="glgB"
                     /locus_tag="VFT03_08840"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU4848292.1"
                     /translation="MRYEDAILSVAAGDHPDPFSFLGMHRSEDGFVVRAFLPGASAVE
                     VVSDSGDVLGALDPVHPDGIFAGPVARMVPYRLRVAWEGDGEAEIEDPYRFPPVFGEQ
                     DLYLFGEGSHLGIYEKLGAHPMELEGVAGASFAVWAPNARRVSVIGEFNLWDGRRHAM
                     RKHPGIGVWEIFIPGVGEGALYKYEIIGPKGDLLPLKADPFGFQAERLPGTASVVHDP
                     SRYEWGDSAWMEGRDRRNALDAPMAVYEVHLGSWRRNPDGSYYTYRQLADELVSYVVE
                     MGYTHVEFLPPTEHPFDGSWGYQPLGLFAPTSRFGTPDDFKHLIDSFHRAGVGVLIDW
                     VPAHFPEDPHGLALFDGTHLYEHADPRQGRHPDWGTLVFNYGRNEVRNYLISNALFWL
                     SEYHVDGLRVDAVASMLYLDYSREPGEWVPNVHGGNENLEAVSFLQRTNELVFGRHSG
                     ATTVAEESTAWPMVSRPVSMGGLGFGYKWNMGWMHDSLQYISQDPINRKYHHDEITFS
                     LIYAFSENFVLPLSHDEVVHGKGSLINKMPGDEWQRFANLRAYYGFMYAHPGKQLLFM
                     GGEFAQSREWNHDTSLDWHLLEGPMHRGVQTLIKDLNALYKATPALYEVDFEPAGFGW
                     IEGGDRENSVVSFLRRGTEAGDLAVFVCNFTPVVRHDYRIGVPEGGRYAEALNTDDPR
                     YGGSG"
BASE COUNT         1134 a         1988 c         1956 g          972 t
ORIGIN      
        1 acgcaggact ggcccaggga gtacatgcgc cccaacttct tccccaacac ccacgacatc
       61 ctgccacaca tcctccagtt cggtgggagg cccgcgttcc agacgcggtt cgtgctcgcc
      121 gccacactgt cgagcaccta cggtatttac agcggcttcg agctgtgcga gaacactccg
      181 aggggcgagc caggcactac cgtgtactac caggactcgg aggtatacga gtacaaggtc
      241 tgggactggg acaggccagg gaacatcagg gactacataa ggagggtgaa cgagatccgg
      301 cgcgacaacc ctgcgctcca ctacctgaag aacctctcct tctacgaagc cccgaacgat
      361 aacgtcctct tctacggcaa acggaccggg gacaataaca tcttcgttgc ggtgaacctc
      421 gacccggacg tggctcacga cacgcacgtg cagttccctc tgtgggagtt cgggatcggg
      481 gagaccgagg gttacgtagt cgaagagcta ataacgcagc aggagatgcc cgccacgggt
      541 agctggttct gggtccggct cgacccggag agcaacccgg cggagatctt ccgcctcagg
      601 aaggcgggtg tgcgatgacg gaggtctcat ccagaggagc gaggggtttc gaggaggatc
      661 cggaggaccc tctctggtac aaggacgcca tcatctacca cctccacgtg aaggctttct
      721 tcgattcgaa caacgacggc ataggagact tcaagggcct cacgcagcgg cttgactaca
      781 taaaagacct tggggtcacg gcgatctggg tgatgccgtt ctacccctcg ccgatgcgcg
      841 atgacggcta cgacatctcc gagtacaaga acgtccacgc cgactacggt accaggcggg
      901 acttcagggc ttttgtccgc cgggcgcacg aactgggcct gaaggtcata accgagctgg
      961 tcatcaacca cacctctgac gcgcacccgt ggttccagcg ggccaggaag gcgcagaagg
     1021 gttcggcgaa gcgtaacttc tacgtctgga gcgacacccc ggacagatac gaaggcaccc
     1081 gcgtcatctt caccgacaca gagcgttcca actggacctg ggacgaggtg gccgagcagt
     1141 actactggca ccgcttcttc tcccaccagc cagacctcaa ccacgacaac ccgcaggtct
     1201 tcaaggcgat tatgggcgtg atgcgcttct ggctcgatgc gggcgtggac ggcctgaggc
     1261 tggacgcgat cccttacctc atcgagcgcg aaggcacgga caacgagaac ctccccgaga
     1321 cgcacgaggt cctcaagagg atgcgggccg agatggacgc ccgctaccac aacagactct
     1381 tcctcgccga ggccaaccag tggcccgagg acgtcctgcc ttacttcggg gagggggacg
     1441 agtgtcacat ggccttccac ttcccgctca tgccccgcat ctacatggcg ctcgcgcagg
     1501 aggacaggca cccgatagtg gagatcatgc accagacgcc cgagatcccc gagatctgcc
     1561 agtgggccat attcttgagg aatcacgacg agctcaccct ggagatggtc accgacaaag
     1621 agcgcgacta catgtaccag acctacgcca cagacccgcg gatgcgcgtg aacgtcggca
     1681 tccggcgccg gctcgccccc atgatggaga acgacaggcc tcgtatagag ctactgaaca
     1741 gcttgctcat gagcatgcct ggcaccccca tcatctacta cggggacgag atcgggatgg
     1801 gcgacaacat cttcctcggc gacagggacg ccgtcaggac gcccatgcag tggacgagtg
     1861 acaggaacgc cggcttctcg cgagccgagc cggcccgcct gtacctgccg cccatcatgg
     1921 acccggtcta cggctacgag tcggtgaacg ccgaggccca gagccgcagc gcgggctcgc
     1981 tgctgaactg gatgaagcgg atcatctcgg ttcgcaaggc gcacaaagcc ttcggacggg
     2041 gaactctgga gttcctgcac cccggcaaca ggaaggtcct cgcctacctg agggaatacg
     2101 aggacgagtc catcctgtgc gtggcgaacc tcagccgctc tgcccagcct gtggagctgg
     2161 acctcgcgcg cttcaggggc cgggtcccgg tcgagctgct cggcaacagc agcttccctc
     2221 ctctgggaga tctgccgtac ttcctcacgc tgcccgggta tagcttctac tggttccgtc
     2281 tggcgacgga ggctgctgcc ccgagctggc accaggagag cccgcggtac gcagagccgc
     2341 ccgtcctggt tctacccgcc gggcgggaga cggcccgcag ggtctacggg gctcctctac
     2401 gcctcccgag ccgccagctc gcacagctcg agagggaggc gctgccgaac ttcctcggca
     2461 cccgccggtg gttcgcggcg aaggggggtg agatcgagcg cgtcgaggtc gcggcgcagg
     2521 gcgagttcga ccacaacggc ctgcttactt tgctccgggt gcacgtggac gaaggtgagg
     2581 ggccgcaact ctatttcctc ccgctcgcgg cggcctggga ggaaggcgcc gatgaggacg
     2641 gcgtctgggc gctcataccc cacgccctgg ccaagctccg gcgccgcgcc aggatgggac
     2701 tcctcctgga cgccatggcc gacgagagct tctgccgcca cgtggtggag gcgatgggga
     2761 agaaccggga tttcggcctc ggggaaggaa aggtccactt ctccgcaacc gaggtcttcg
     2821 gggagttggc cggcgaggag ccgctctccg agcttggcgt acgtcgcgcc gaggtggagc
     2881 agaccaacag ctcggtcatc ctcggcgaga ggctcatact gaaagcgtac cggcgcctac
     2941 agaagggtac gaaccccgac ctggagatcg ggcgctacct gaccgaggag gtccgcttcg
     3001 agaacacgcc gccgctcgcc ggagccgtcg agtaccaggg gcccgacggc gagacgacta
     3061 ccctcggcct cttgcagggc ttcgtggaga gccagggcga cggctggtcc tacgtcctcg
     3121 actacctggg ccgctacctg gaggaccgcc tggtgacctc gtggagcctc gagggcgagg
     3181 agggcgccga ggcggagcgg gaggagcgga gcgccgaaga cgctttcttc gcgagcctca
     3241 tgcgcactct agggttgcga accggcgagc tgcacgccgc cttcgccgcg ccgacctacg
     3301 acacggcctt cgccccggag ctggcgccac cagaggaggt ggccggctgg gccgaggcca
     3361 tactcgcgga gctcaagcgt acgttcgaca cgctcaaacg tcgccgggac aggctgccgg
     3421 aggaggtggt cccggacgcg tacaggctcc tgggactgcg cggggagttg acgaggcgcg
     3481 taaaggacgt ctcaagagga ggcttcggcg tcgtaaagac ccggcatcac ggtgattacc
     3541 acctcggaca ggtgctcgtg gtgggcaacg acttccagat catcgacttc gaaggggagc
     3601 cagcccggcc tctcgccgag aggcgcagga aacactctcc tctgcgggat gtggcgggga
     3661 tgctccgctc tttcgactac gccgcccggt cggccgtgat gaacctcggt gccgagcgcg
     3721 ctgagcagct cgagagcctc gagccttggg tccggctctg gcaggagcgc gcgcgggagg
     3781 ctttcctcga agggtacgcc gagggcgcgc gcggggccgc ttcctacccc gacgacgagg
     3841 agcacgccag gacgctcgtc gagctcttca ccctcgagaa ggccctctac gagatccgct
     3901 acgagctgga caaccgcccg gactgggtag gtatccccat cgggggcatc ctggatctcc
     3961 tgggagaacc ggtcggggag gaggagtctt gaggtacgaa gacgcaatcc tctccgtcgc
     4021 ggccggcgac caccccgacc ctttctcctt cctcgggatg caccgttcgg aggacggctt
     4081 cgtggtgcgg gcctttctgc cgggagcgag cgcagtggag gtcgtctccg atagtggaga
     4141 tgtcctgggc gcgctagatc ctgtgcaccc ggacgggatc ttcgccggcc ccgtcgcgcg
     4201 gatggtgcca taccggctga gggtcgcgtg ggagggagac ggagaagctg agatcgagga
     4261 cccttaccgt ttccccccgg ttttcggcga gcaggacctc tacctcttcg gcgaaggcag
     4321 ccacctcgga atctacgaga agctcggagc gcacccgatg gagctcgaag gcgtcgccgg
     4381 cgcgagcttc gccgtctggg cgccgaacgc ccggcgcgtc tcggtgatcg gggagttcaa
     4441 cctgtgggat ggacggcggc acgctatgcg aaaacacccg gggattgggg tgtgggagat
     4501 cttcatcccg ggggtcggcg agggcgctct atacaagtac gagatcatag gccccaaagg
     4561 cgacctcctg cccctgaagg ccgacccctt cggcttccag gccgagaggc tccccggcac
     4621 cgcctccgtc gtccacgatc cctcgcgcta cgagtggggc gactcggcct ggatggaggg
     4681 cagggaccgc aggaacgcgc tcgacgcccc catggctgtc tacgaggtgc acctcggttc
     4741 ctggagaagg aaccccgacg gctcctacta cacctaccgc cagctcgccg acgagctggt
     4801 ctcctacgtc gtcgagatgg gctacaccca cgtcgagttc ctgccgccga ccgagcaccc
     4861 gttcgacggc tcctggggct accagccact gggtctcttc gccccgacga gcaggttcgg
     4921 cacgcccgac gacttcaagc acctgatcga ctccttccac agggccgggg taggcgtgct
     4981 gatcgactgg gtcccggcgc acttcccgga agatccccac ggcctggctc tcttcgacgg
     5041 cacccacctc tacgagcacg ccgaccctcg ccagggcagg caccccgact ggggaacgct
     5101 cgtcttcaat tacgggcgca acgaggtgcg caactacctc atctccaacg ccctcttctg
     5161 gctctccgag taccacgtag acgggttgag ggtcgacgcc gtagcctcga tgctctacct
     5221 ggactactcg cgcgagcccg gcgagtgggt cccgaacgtg cacggcggta acgagaacct
     5281 cgaggccgta agcttcctcc agcgcacgaa cgagctggtc ttcgggcggc actccggcgc
     5341 cactaccgtc gcggaggagt caacagcctg gccgatggtc agccgccccg tctcgatggg
     5401 tggcctgggt ttcggctaca agtggaacat gggctggatg catgacagcc tgcagtacat
     5461 ctcgcaggac cccatcaacc gcaagtacca ccacgacgag atcacgttct ccctgatcta
     5521 cgccttcagc gagaacttcg tcctgccgct ctcccacgac gaggtggtgc acggaaaggg
     5581 ctcgctcata aacaagatgc ccggagacga gtggcagagg ttcgccaacc tccgcgccta
     5641 ctacggcttc atgtacgccc atccaggcaa gcagctcctg ttcatgggag gcgagttcgc
     5701 gcagagcagg gagtggaacc acgatacgtc tctcgactgg cacctgctcg agggtcctat
     5761 gcacaggggc gtccagaccc tgataaaaga cctcaacgcc ctctacaaag ctacccccgc
     5821 cctctacgag gtggacttcg agcccgcggg gttcgggtgg atcgaaggtg gggaccgcga
     5881 gaacagcgtg gtatcctttc tgcgccgcgg cacagaggcc ggggacctcg cggtcttcgt
     5941 ctgtaacttc acgccggtcg tccggcacga ctaccgtatc ggggtgcccg agggcggccg
     6001 ctacgccgag gcgctcaaca ccgacgaccc ccgctacgga ggcagcggtg
//
LOCUS       DASTUV010000438         5490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1656481, whole genome shotgun sequence.
ACCESSION   DASTUV010000438 DASTUV010000000
VERSION     DASTUV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656481"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2499
                     /gene="mfd"
                     /locus_tag="VFT03_08845"
     CDS             <1..2499
                     /gene="mfd"
                     /locus_tag="VFT03_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU4848293.1"
                     /translation="DPVSPRRLLPDGVEVWREAPQERIPDDLEGAVRELYDPRTPEPD
                     VEFTPGEGGEVVAAQPVATFADTLREAARRLDSLVEQGLAVFVACSSVGEAKRTVYAF
                     GEIHRAMREVGRVDASLPPGLYAVGREVEEGFVYPDGGVAVVRRASLFGRRREARRRP
                     GRALGSFADLKAGDLVVHAAQGIGRFEGLVAREVLGTTRDYMQVTYRGGDTLLVPYEQ
                     MELLHKYVGSEVARLDRLGGATWAQVTDRVRSRVKALAGELLQLYAARASTPGFAFPE
                     DSEWERELEESFPYQETTDQEAAIAAVKADMRVPHPMDRLVCGDVGFGKTEVAVRAAL
                     KAALSGKQTMMLAPTTILVQQHERTFKERLGTFAVRVESLSRFTTPAARRAILRDFAA
                     GEVDVLIGTHALLGTEVTPRDLGLVVVDEEQRFGVRHKERIKELRTSVDVLTLTATPI
                     PRTMQMGLASLRDISVIETPPAGRRSILTHVGPYDEGLVRLAIGREVAREGQVFFVHN
                     RVETIDEVAEKLRSLVPGVRFVVAHGQMPERALEDVMRGFLEGEADVLVTTTIVESGL
                     DVATTNTLIVDRSDTMGLAQLYQLRGRIGRSTEQAYAYLFAPLGATVESQKRLEALMD
                     FTELGSGFAIAMRDLEIRGAGNLLGAEQSGHIAAVGFEMYLRLLEEAVAAARGEAPER
                     RDRRPVIVELPLDAYLPPAYVMDEIERVDLYRRASGTASIAEVDDLAEELADRFGEPP
                     EPALNLLGLSRIKILAREAGASSVTYRSGTLAVVGVTAGPAQTALLEERTGGAVGGRE
                     GRVTVRDSALEPLALAESVLRALNQRPVRKLTDR"
     gene            2648..3658
                     /locus_tag="VFT03_08850"
     CDS             2648..3658
                     /locus_tag="VFT03_08850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4848294.1"
                     /translation="MSEFLVNRAWFLCVLVGVLVVVAAGCSAAEPVANIESPSKKVAV
                     FEGGEVTLGEVQEFAEQSGLGELSPDSPQYEALIAQIMPQLTDIEIAKAYAEEHGITV
                     SEQDVDKEIETLKDQVFQQAQSQGQDVGREEAFDQALQQAGVTEEQLRQQIRDQLPIQ
                     KVQERVAGDVGPSQEEVEQFYEQNKAAQFTTPEARCTRHILFNKDQKAKAEEVKQQLQ
                     NGGDFAELAKEYSQDPGSAEQGGDLGCIGRGETVPNFEEAVFNAKQGEIVGPVETEFG
                     YHLIEVTEIHEEATQPLSEVEGQIREQLTTEAQSQEFTAWLQKQREQRNVKYLPGYKP
                     PA"
     gene            3808..5103
                     /gene="eno"
                     /locus_tag="VFT03_08855"
     CDS             3808..5103
                     /gene="eno"
                     /locus_tag="VFT03_08855"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU4848295.1"
                     /translation="MTEIVAVHGREILDSRGNPTVEVELATVSGFVGRASVPSGASTG
                     QNEAVELRDGDPERYSGKGVLSAVANVDGELAQVVVGMDVTDQRSLDLAMIEADGTEN
                     KGRLGANAILGISLAAAKAAAEAAALPLFRYLGGPGAYTLPVPCANIMNGGAHAANNV
                     DFQEFMVVPLGFEDYSEALRAVAEIYQGLKKLLGERGLAGGIGDEGGFAPDLEDNGAA
                     LALIAEAVERSGYSLGDQIGFALDPAASEFYESGSYVLEGEGKTLSREEMVEYYVRLC
                     DRYPIISIEDGLAEDDWEGWAMITERLGSRIQLVGDDLFVTNPKILARGIHEGIANSI
                     LIKVNQIGTLTETLETIELAHKAGYTAMISHRSGETEDVTISDLAVATNAGQIKTGAP
                     ARTDRVAKYNQLLRIEEGLEGSGFYPGRAAFNPRVGKGV"
     gene            5106..>5490
                     /locus_tag="VFT03_08860"
     CDS             5106..>5490
                     /locus_tag="VFT03_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU4848296.1"
                     /translation="MRRTKIIATLGPATSSEEKIEALIRVGVDVTRFNFSHGDHQMHL
                     KNAEIVRSAAKEQGRNVAIMQDLQGPKIRTGEVEEGTELVEGNRVVIASGDFVGDASR
                     LSTTYDRLAEDVEPGDRLLIDDGLIG"
BASE COUNT         1035 a         1698 c         1925 g          832 t
ORIGIN      
        1 gaccccgtct cgccgcggag gttgctgccg gacggagtcg aggtgtggcg tgaggcgccg
       61 caggagagga tcccggacga cctcgaggga gcggtgcgcg aactctacga tccgaggacg
      121 ccggagccgg acgtcgaatt cacgcccggc gaggggggag aggtcgtcgc agcccagccg
      181 gtcgcgacgt ttgccgacac tctgagggag gcagcgaggc ggctcgactc gctggtggaa
      241 cagggtctcg cggtcttcgt agcctgctcc tccgtcgggg aggcgaagcg gacggtctac
      301 gccttcgggg agatacaccg cgcgatgcgc gaggtcgggc gggtggacgc gagcctgccg
      361 ccgggcctct acgcggtggg acgcgaggtg gaggaggggt tcgtataccc tgacggtggg
      421 gtcgcggtcg tccgccgcgc ttcgctcttc ggtcggcggc gcgaggcgcg ccggcgtccg
      481 ggtcgcgcgc tcggctcctt cgcggacctg aaggcagggg acctggtggt tcacgcggcg
      541 cagggcatcg ggcgcttcga gggtctggtt gcgagggagg tcctcgggac cacccgcgac
      601 tacatgcagg tcacataccg cggcggggac accctgctcg ttccctacga gcagatggag
      661 ctcctgcaca agtacgtcgg cagcgaggtc gcccgtctcg acaggctcgg cggggcgacc
      721 tgggcgcagg tcacagacag ggtacgcagc agggtcaagg cgctcgctgg tgagctactg
      781 cagctatacg ctgcgagggc ctccacgccc ggcttcgctt ttcccgaaga cagcgagtgg
      841 gagagggaac tcgaggagag cttcccctac caggagacta ccgaccagga agctgccatc
      901 gccgcggtca aggcggatat gcgggtgccc cacccgatgg accggctcgt gtgcggtgac
      961 gtcggtttcg gcaagaccga ggttgccgtg cgggccgccc tcaaggcggc gctgagcggc
     1021 aagcagacca tgatgctcgc gccgacgacc atcctcgtgc aacagcacga gcggaccttc
     1081 aaggagaggc tcggcacctt cgccgtccgc gtcgagagcc tctcgcgctt cacgacgcca
     1141 gccgcgcgca gggccatcct gcgcgacttc gcagcgggcg aggtggacgt tctcatcggt
     1201 acccacgcgc tccttggcac ggaggtcacg cctcgcgacc tcgggctcgt ggtggtggac
     1261 gaggagcagc gcttcggcgt gaggcacaag gagcgcatca aggagctcag aacctccgtg
     1321 gacgtcctca cgcttacggc caccccgatc ccgcgcacca tgcagatggg tctcgcctcc
     1381 ttgcgggaca taagcgtcat cgagacgccg ccagccgggc ggcggagcat cctgacgcac
     1441 gtcggcccct acgacgaggg cctcgtcagg ctggccatag ggcgggaggt cgcgcgcgag
     1501 ggacaggtct tcttcgtcca caaccgcgtc gagacgatag acgaagtggc cgaaaaacta
     1561 cgctccctcg tgcctggggt gcgtttcgta gtcgcgcacg gccagatgcc ggagagggcg
     1621 ctcgaggacg tcatgcgggg cttcctcgaa ggcgaggccg acgtgctcgt gaccacgacc
     1681 atcgtggaga gcgggctcga cgtggcgacc acgaacaccc tgatcgtgga ccgctcggac
     1741 acgatgggtc tcgcgcagct ctaccagttg cgcggcagga taggacgctc caccgagcag
     1801 gcctacgcct acctcttcgc tcctctggga gccaccgtcg agtcgcagaa gcggctcgaa
     1861 gcgctcatgg acttcaccga gcttggcagc gggtttgcca tcgcgatgcg ggacctggag
     1921 atccggggag ctggcaacct cctcggcgca gagcagtcag gccacatagc cgcggtcggc
     1981 ttcgagatgt acctgcgact cctcgaagag gcggtagctg ccgcgagggg cgaggcgccg
     2041 gagcgcaggg ataggcgacc agtcatcgtg gagctcccgc tcgacgcgta cctgccgccc
     2101 gcctacgtga tggacgagat cgaacgcgtg gacctctacc ggagagcctc aggtacggcg
     2161 tccatcgccg aggtggacga cctcgccgag gagctcgccg accgcttcgg cgagcctccg
     2221 gagccagcgc tgaacctgct cggcctctcc aggataaaga tcctggcccg cgaggcgggc
     2281 gcgagctccg tcacctaccg ctcgggaact ctggccgtcg tgggcgttac cgccggtccg
     2341 gcgcagacgg cgctcctcga ggagcggacc ggcggcgcgg taggtggtag ggagggacgg
     2401 gtcacggtgc gcgactcagc cctcgaaccg ctcgcgctcg cggagagcgt tctacgcgcg
     2461 ctgaatcaga gaccggtcag aaaactcacc gatcgctaat gatcctggcg ccgctcgtcc
     2521 agcggtacat cgtgagtact ttgcgaactg tagagcgttg ccctccgttc tcgtgtgctg
     2581 gccggcgcgg gctccgtgct ataatcgcgg cgtttttggc atacgaacag tgaggttctc
     2641 cgtcgtcttg agcgagtttc tggtgaacag ggcgtggttc ttgtgcgtgc tcgttggcgt
     2701 gttagtggtc gtcgccgcgg gttgtagcgc ggccgaacct gtcgccaaca tcgagtcgcc
     2761 ttccaagaag gttgcggtct tcgaaggagg agaggtcacc ctgggcgagg ttcaggaatt
     2821 cgccgagcag agcggtctgg gcgagctttc gcctgactct ccacagtacg aggcgctcat
     2881 cgcccagatc atgccgcaac tcacagatat cgagatcgcc aaagcctacg cagaggagca
     2941 cgggataacg gtctcggagc aggatgtcga taaggagatc gagaccctca aggaccaggt
     3001 cttccagcag gcgcagagcc aggggcagga cgtcgggcgc gaggaagcat tcgaccaggc
     3061 cttgcagcag gcgggcgtca ccgaggagca gctacggcag cagatccggg accagctccc
     3121 catccagaag gtacaggaga gggttgcggg cgacgtcgga ccttcgcagg aagaggtcga
     3181 gcagttctac gagcagaaca aggcagccca gttcacgact cccgaggcgc gctgcacgcg
     3241 gcacatcctg ttcaacaagg atcagaaggc gaaagccgag gaggtcaagc agcaactcca
     3301 gaacggcggg gacttcgctg agcttgccaa ggaatactcc caggacccgg gtagcgccga
     3361 gcagggcggc gacctcggct gtatcggcag gggtgagacc gtccccaact tcgaggaggc
     3421 ggtcttcaat gccaagcagg gtgagatcgt gggtccggtc gagaccgagt tcggttacca
     3481 cctcatcgag gtcaccgaga tacacgagga ggcgactcag ccgctctccg aggtcgaggg
     3541 tcagatcagg gagcagctca ccaccgaggc gcagtcccag gagttcacgg cctggctgca
     3601 aaagcagcgt gagcagcgca acgtcaagta cctgcccgga tacaagccgc ccgcgtgaaa
     3661 acctcctccc caggctgtat acggtggcgc cgcaacgggt aagagcgtag agttacaaca
     3721 aagctgatca gctaatagga ggtttaaggc tacaggcatc aggtaaaacc cataacctga
     3781 aacctgatag ctcaaagagg gaggatgatg acggagatcg tcgctgtcca cgggcgtgag
     3841 atcctggact ccagggggaa cccgacggtc gaggtcgagc tcgccacggt gagcggcttc
     3901 gtgggccggg cctctgtccc ctcgggcgcc tcgacaggcc agaacgaggc cgtcgagctg
     3961 cgggatgggg accctgagcg ctacagcggc aagggggtct tgagcgccgt agcgaacgtc
     4021 gacggcgagc tggcgcaggt cgtcgtcggt atggacgtaa ccgaccagcg ctcgctcgac
     4081 ctcgccatga tcgaagccga cggcacggag aacaagggcc gcctcggggc gaacgctata
     4141 ctcgggatct ccctggccgc ggcgaaggcc gcagccgagg cggccgcgct gccgctcttc
     4201 cgctacctcg gcggtccagg agcgtacacg ctgcctgtcc cctgcgccaa catcatgaac
     4261 ggtggcgcgc acgcggctaa caacgtggac tttcaggagt tcatggtcgt gcctctgggc
     4321 ttcgaagatt actccgaggc gctgcgggcg gtggcggaga tctaccaggg cttgaagaag
     4381 ctgctcgggg agaggggcct cgcgggcggc atcggggacg aaggagggtt cgcccccgac
     4441 ctcgaggaca acggagcggc gctcgcgctg atcgccgagg ccgtcgagag gagcgggtac
     4501 tcgttgggcg atcagattgg cttcgccctc gaccccgcgg cctccgagtt ctacgagagt
     4561 ggctcatacg tcctggaggg cgaaggcaag accctctcgc gcgaggagat ggtcgagtac
     4621 tacgtccgac tgtgcgaccg gtatcctata ataagcatcg aggacgggct tgccgaagac
     4681 gactgggagg ggtgggccat gatcaccgag cgcctgggga gcaggatcca gctagtcggc
     4741 gacgacctct tcgtcacgaa cccgaagatt ctcgcccgcg gtatccacga gggcatagcc
     4801 aactccatcc tcataaaggt caaccagatc gggacgctca ccgagaccct ggagacgata
     4861 gagctcgcgc acaaggctgg ctatacggcg atgatcagcc accgcagcgg ggagaccgag
     4921 gacgtgacga tctccgacct cgcggtggct accaacgcgg gtcagataaa gaccggagcc
     4981 cctgcgagga ccgaccgcgt cgccaagtac aaccagctac tcaggataga ggaggggctc
     5041 gagggctcgg gcttctaccc cggccgggcg gcgttcaacc cgagggtggg gaagggggtc
     5101 tgaaggtgcg ccggaccaag ataatcgcca ctctagggcc cgcgacctcc tcagaggaga
     5161 agatcgaggc cctcatccgg gtcggggtag acgttacacg cttcaacttc agccacggcg
     5221 accaccaaat gcacctgaag aacgccgaga tcgtgcgctc ggcggcgaag gagcaggggc
     5281 gcaacgtggc gatcatgcaa gacctccagg gccccaagat taggacgggg gaggtggagg
     5341 aaggcactga gctcgtcgag gggaacaggg tcgtcatcgc ctcaggggac ttcgtcgggg
     5401 acgcgagccg gctctccact acctacgacc gcctcgcgga ggacgtggag cccggcgacc
     5461 ggctcctgat cgacgacggc ctcatcggcc
//
LOCUS       DASTUV010000439         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_240876, whole genome shotgun sequence.
ACCESSION   DASTUV010000439 DASTUV010000000
VERSION     DASTUV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240876"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFT03_08865"
     CDS             complement(<1..185)
                     /locus_tag="VFT03_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848297.1"
                     /translation="MVSSEQQKRLAVGWFSFTCCEDSTILLTELLNDHFEEWRKVIEF
                     RHMKALRTNNSLVGLDVA"
     gene            complement(179..1252)
                     /locus_tag="VFT03_08870"
     CDS             complement(179..1252)
                     /locus_tag="VFT03_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4848298.1"
                     /translation="MSDDQSKEALRVLEQSFGDRVKQSPVRGEGPGTEAALASVLPMS
                     AEEVRLLAEIAGRFSVPLVALGGGTISEEAVEKGSILVRFDLMRRTQLPDPEEQWAEA
                     EPGVSWLQLDDELRLLGRGLAVYPTSAPRATVGGWLAMDGLGVGSFEYGWLRENVLSA
                     SVVLPGGELAEVAGEEVRSFVGPGAAGIIVDARLRTRRADADVLFGAAFGSAAELVGA
                     VAGMVEAGLPLWHLAFLNPGMAHARNLTEGFLLFGAYPAEREGKVEGSLRSAVASSRG
                     RVLSAAEAHRAWGERFFPVTPSILPPRFPGVSRELVSLAELPAKLSAVEDHPEHAILQ
                     GTVARSEEVLLLTLEDHEECTAW"
     gene            1556..1819
                     /locus_tag="VFT03_08875"
     CDS             1556..1819
                     /locus_tag="VFT03_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848299.1"
                     /translation="MEEARERTIGRHVRMAHATIKGEHQLAEYVARLEIGECARILAV
                     APESLRLLATRRDECRVGPGERRSFEEVIRTWTEFVLSGVEGG"
     gene            1866..2819
                     /locus_tag="VFT03_08880"
     CDS             1866..2819
                     /locus_tag="VFT03_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4848300.1"
                     /translation="MFPTKILLATDGSAEATRAARMAITLSKELDSELHIVYVEPLPD
                     PFATPEFLSRYPDFRDELRKIAERQATEKLDKEAEKIREMGKVAGTHARIGRPDAEIA
                     SLAEEIGAGLVVVGSRGLGRIRRALMGSISDSVVHHAYCPVLVVRDGQGEYLPGRILL
                     ALDGSEEAEAAARAAMEISNATGSELHIVFALRTDPQLPYPYPLLGERWGSIIEQAKR
                     DARMFVDAMAEEIEAEGCKVKDAHLVFGKPEAEIVKLGEELEAGLIVVGSRGLGGVDR
                     ALMGSVSDSVVRHAHGPVLVVRSGERQREAVARTAEERAQT"
     gene            complement(2906..>3550)
                     /locus_tag="VFT03_08885"
     CDS             complement(2906..>3550)
                     /locus_tag="VFT03_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848301.1"
                     /translation="VLIGKKKATMRDYAPEVPEREFGLPVVLLVGTSMSAGKTTSAKV
                     IVRVLREEGLAVVGAKLTGAGRYRDILSMQDAGAEHIFDFVDAGLPSTVVPEKEYRQV
                     LRRLLSRIATADADVLVAEVGASPLEPYNGAAAIDEIGPNVRCTVLSASDPYAVTGVT
                     SAFGNRPDLVAGLATSTSAGIELVEKLSGIKALNVLDRSSLTRLRTILKDTLDF"
BASE COUNT          665 a         1153 c         1132 g          600 t
ORIGIN      
        1 gccacgtcga gtccgacgag cgagttgttc gtcctgagcg ccttcatgtg ccggaactcg
       61 atcacctttc tccattcttc gaaatggtcg ttgagcagct ccgtaaggag gatggtgcta
      121 tcttcacaac acgtgaatga aaaccagcct acagccagtc gcttctgctg ctcactgctc
      181 accacgccgt acattcctcg tgatcctcta aagtcaggag caacacctct tcagaccggg
      241 cgacggtgcc ctggaggatg gcgtgctcgg gatggtcctc caccgcactc agcttcgctg
      301 gcagctccgc caacgacact agctcgcggg atacgcctgg aaaccgcggg ggaaggatag
      361 agggcgtgac ggggaagaat ctctcccccc atgcccggtg ggcctccgct gcagagagga
      421 cgcgacctcg ggaggatgcg acggcgctgc ggagactgcc ttcgaccttt ccttctcgtt
      481 ctgccgggta ggctccgaag agcaagaagc cttccgtaag gtttctggcg tgtgccatgc
      541 cggggttcaa gaaggccagg tgccagagcg gcaagcccgc ttcgaccatg cccgccacgg
      601 cgccgacaag ctcagctgcg gatccgaagg cggcaccgaa gagcacatcc gcgtcagccc
      661 gccgggtgcg caacctcgca tccacgatga tgcccgccgc cccgggcccg acgaaagacc
      721 gcacttcctc ccccgccacc tcggcgagct ctccgcccgg cagcaccacg ctcgccgata
      781 ggacgttctc ccgcaaccac ccatactcga aggaacccac gccgaggccg tccattgcca
      841 gccagccacc gactgtggcc cgtggggcgc tcgtggggta gacggccagg cccctgccca
      901 gaaggcgcag ctcgtcgtcc agctggagcc aggatactcc gggctccgcc tcagcccact
      961 gctcttcggg gtcgggcagc tgcgtgcggc gcatcaggtc gaaacggacc aagatactgc
     1021 ccttctctac agcctcttca ctgatcgtcc caccgcccag cgctaccagc ggcactgaga
     1081 aacgtcccgc tatctcagcg agaaggcgaa cctcctcggc gctcatcggc aggacggacg
     1141 ccagcgccgc ctcggttcca ggcccctccc ctcgaaccgg gctctgcttg acccggtccc
     1201 cgaacgactg ctccaggact ctcagggcct ccttactctg gtcatcactc atccgcaccc
     1261 ctttgtttcg ttagtttcgt tatcttctga gggcaggtta ctacgaagtc cgcgtctact
     1321 cctgatcgat cctggccaaa gtctcatcga gattgaccat cttccccgca actcgcgacg
     1381 accggccttt ttagtcattt cttgccagca ggttggtcca atttgctcag ggcgtcgtgg
     1441 ccaccgcagg ctacagtggt cgcggatgag cagatggcga aacggagcgg aggcacagga
     1501 ctggagaggt ctcaagtcct gtcccgcaag cctggcgtaa cgggaggcga agatcgtgga
     1561 ggaggcacgc gaacgcacca tcggcagaca cgtcaggatg gcgcacgcga caatcaaggg
     1621 tgaacaccag ctcgcagagt acgtggcgcg actcgagatc ggggagtgcg ccaggatcct
     1681 cgcggtcgct ccagagagcc tacgcctgct ggccacccgc agagacgagt gcagggtcgg
     1741 tcccggcgaa aggcggagct tcgaggaggt gatcaggacc tggaccgagt tcgtgctctc
     1801 gggagtggag ggcggctaga taaccgaggc tatccacacc aatgactcga aggaggaatt
     1861 aaccgatgtt cccgacgaag atactgctcg cgaccgatgg atccgcggag gcaacccgcg
     1921 ccgcgaggat ggcaataacg ctctcgaagg aactcgactc cgagctgcac atcgtctatg
     1981 tcgagcctct accggatccc ttcgccaccc cggagttcct ctcccgctat cccgactttc
     2041 gggatgagct acgcaagatc gccgagcgcc aggcgaccga aaagctcgac aaagaggcgg
     2101 agaagataag ggagatgggc aaggtagcgg ggacccacgc caggatcggc cgtcccgacg
     2161 cagagatcgc aagcctcgcc gaggagatcg gcgcggggct tgtggtagtg ggcagcagag
     2221 gcctgggacg cataaggcgt gctctcatgg gcagcatctc cgactccgtc gtccaccacg
     2281 cctactgccc cgtgctagtg gtgcgagatg gccagggaga gtacttgcca ggcaggatcc
     2341 tgctggcgct cgacggctcc gaggaggccg aagcggccgc ccgggccgcc atggagatct
     2401 cgaacgccac tggttcggag ctgcatatcg tcttcgcctt gcggaccgac ccgcagctac
     2461 cctacccgta ccctctcttg ggcgagaggt ggggctccat aatcgagcag gccaagcgcg
     2521 acgcccggat gttcgtggac gctatggctg aggagatcga ggccgaaggt tgcaaggtca
     2581 aggacgcaca cctcgtcttc gggaagcccg aagcggagat cgtcaagctg ggtgaggagc
     2641 tcgaggcggg tctgatcgtg gtcggcagcc ggggcctggg cggagtggac agggctctga
     2701 tgggtagcgt ctccgactcg gtggtgcgcc acgcccacgg cccggtcctc gtcgtcagga
     2761 gcggtgagcg ccagcgtgag gctgtcgcca ggacggctga agagcgagca cagacctgaa
     2821 ccgatgagtg ctctggcggg gaggatcata ctggctaccg acggctccga ggacgtggcg
     2881 atggccgcag gaaggtcgcg gaggtttaga agtctagcgt gtctttcagg atagttctta
     2941 acctagtaag cgagctccgg tccaggacgt tcagggcctt gatgccggag agcttctcga
     3001 cgagctcgat gcccgcggag gtgctcgtgg cgaggccggc cacgagatcc gggcggttcc
     3061 cgaaggcgct ggttacgccg gtcacggcgt aggggtccga agcggatagc acggtgcaac
     3121 gcacgttcgg cccgatctca tctatcgccg ccgccccgtt gtagggttcc aggggcgagg
     3181 cgcccacctc tgccacgagc acgtcggcgt ctgcggtggc tatgcgcgag aggaggcggc
     3241 gcaagacttg ccggtactct ttctccggaa ccacggtgga aggcaagccc gcgtccacga
     3301 agtcgaagat gtgctccgcg ccagcgtcct gcatcgagag gatgtcccgg tagcgccccg
     3361 ccccggtcag cttcgccccg actaccgcga ggccctcctc cctcaggacg cgcacgatga
     3421 ccttcgccga tgtggtcttc cccgcggaca tcgaggtccc gacgagcagc accacaggga
     3481 gcccgaactc gcgctctggg acctccggtg cgtagtcgcg catcgtcgct ttcttcttcc
     3541 cgatgagaac
//
LOCUS       DASTUV010000440         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_883036, whole genome shotgun sequence.
ACCESSION   DASTUV010000440 DASTUV010000000
VERSION     DASTUV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883036"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..2230)
                     /gene="ligA"
                     /locus_tag="VFT03_08890"
     CDS             complement(233..2230)
                     /gene="ligA"
                     /locus_tag="VFT03_08890"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU4848302.1"
                     /translation="MAESVDVRSRIEGLREQVRYHNRRYHIEDAPEISDAEYDALYAE
                     LESMEAEHPELVTPDSPTQRVGGEPLEGFEEVRHTVPMLSLANARKTEDLREWDARVR
                     RLLGPEEEEQLRYVTELKIDGLAVSLRYENGHFVRGATRGNGIVGEDVTQNLRTVRAI
                     PDRLDDEPPEILEPRGEVFMTLGNFEELNRRQEAEGKPPFANPRNAAAGAIRQLDSRI
                     TASRPLTIYLYGVGEGGELYESHSAMLDALKSYGLRANPHRLHDSIETVIEECERSAA
                     ERESLPYQIDGVVVKVDSREQQRSLGTVARAPRWAIAYKFEPLAGRTKLLDIIVKVGR
                     TGALTPQSVLEPVNIGGVMISRATLHNEDYVKEKGILIGDTVVVERAGDVIPQVVRPV
                     VEERTGDERAFAMPTHCPVCGEPVSRPEGEAVTRCVNARCPAQALEHIIHWSSKGAMD
                     IDGLGEKVATRFFDLGLIRDPADIYDLREEQISPLEGFGEKSAENLIRGIERSKEQPF
                     PRVLYALGIRHVGSVTAELIAARFGGEDLLRGVSVEQLTEINGVGEVVARAVVEYFAL
                     DDNRTLVERLMRVGLHFERVASTSDGPLAGKRMVITGTLSRPRGEFVERLEAAGGTFT
                     SSVSKNTDFVLAGEEAGSKLERARELGVPVIDEAGFEELVS"
     gene            complement(2273..>2563)
                     /locus_tag="VFT03_08895"
     CDS             complement(2273..>2563)
                     /locus_tag="VFT03_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEU4848303.1"
                     /translation="EIGANVPDSGSGTYLAVYWGEKPTGGYSMSVRSARLEGSRVTVR
                     LALKEPPRDAILTQALTYPYAVAVIRELDPGGKDFSFVDENGRALGWPVRRV"
BASE COUNT          384 a          885 c          821 g          473 t
ORIGIN      
        1 atcggggcag gcggcggagc tgcggccgcg ctcctgctct ggcggctggc aagctaccat
       61 gcgatcttcc tcctcggccc gccggcggcc tggcttctct acctctcgaa gccggtgatg
      121 aacagcggtg tctcggagca cgaacgagga aaggggcctt gaggtggcgt ttcaaaacct
      181 gcctggtcga ggtctataac ctggcaatcc gaacgggcag gagcaagggt ttttacgaga
      241 ctagctcctc gaagcccgcc tcgtcgatca caggcacgcc cagctctctg gcccgctcca
      301 gcttcgagcc ggcctcctcg cctgccagta cgaagtcggt gttcttgctg acagaggagg
      361 tgaaggtgcc gcccgcggcc tcgagcctct cgacgaactc gccgcgcggc ctgctcaagg
      421 tgccggtaat aaccattctc ttgcctgcca gaggaccgtc ggaggtactg gctaccctct
      481 cgaagtggag gccaacccgc atcaggcgct cgaccagagt cctgttgtcg tcgagggcga
      541 agtattcgac cacggcccgc gccacgactt cgccgacgcc gttgatctcg gtcagctgct
      601 ccacgctcac cccgcgcagc aggtcctcgc cgccgaaacg cgcagctatg agctcggccg
      661 tcacggagcc gacgtgccgg atacccagcg cgtaaaggac gcgggggaag ggctgctcct
      721 tgctcctctc gatgccccgg atgaggttct ccgcgctctt ctcgccgaag ccttcgaggg
      781 gggagatctg ctcctccctg aggtcgtaga tgtcagcggg gtctcttatg aggccgaggt
      841 cgaagaagcg ggtcgcgacc ttctccccga gaccgtctat gtccatagcc cccttcgagg
      901 accagtggat gatatgctcc agcgcctggg ccgggcagcg ggcgtttacg cagcgcgtga
      961 cggcctcgcc ctcggggcgg gagacaggct caccgcagac ggggcagtgc gtgggcatcg
     1021 cgaaggcgcg ctcgtcgccg gtgcgctcct cgactacggg acgcacgacc tgcgggatga
     1081 cgtcccccgc ccgttcgact accaccgtat cgccgatgag gatgcccttc tcctttacgt
     1141 agtcctcgtt gtgcagcgtg gcgcgcgaga tcatcacccc gccgatgttc accggctcga
     1201 ggacagactg cggcgtgagc gcccccgtgc gcccgacctt gacgatgatg tcgaggagct
     1261 tcgtgcggcc agcgagcggc tcgaacttgt acgctatggc ccaccgcggc gccctggcga
     1321 ccgtgcctag agagcgctgc tgctcgcgcg agtcgacttt caccacgacg ccgtctatct
     1381 ggtaggggag cgactctctc tccgcggcgc tgcgctcgca ctcctcgatc actgtctcta
     1441 tggagtcgtg cagccgatga gggttcgccc gtagcccgta gctcttcaaa gcgtctagca
     1501 tggcggagtg gctctcgtac agttcgccgc cctccccgac gccgtagagg tagatggtca
     1561 ggggcctcga agccgtgatc ctcgagtcga gttgccggat ggctcccgcc gcggcgttgc
     1621 gcgggttggc gaagggcggc tttccttcgg cctcctgccg ccggttgagc tcctcgaagt
     1681 tcccgagcgt catgaacacc tcgccccgcg gttccaggat ctctggcggc tcgtcgtcga
     1741 gcctgtccgg tatggcgcgc accgtccgca agttctgggt cacgtcctcg ccaactatcc
     1801 cgttgcccct cgtcgcgccc ctgacgaaat gcccgttctc gtagcgcagc gagaccgcga
     1861 gcccgtctat cttcaactcc gtaacgtagc ggagctgctc ctcctcttcc gggccgagga
     1921 gccgcctgac gcgggcgtcc cactccctca agtcctcggt cttgcgggcg ttcgccaggg
     1981 atagcatcgg gacggtgtgg cggacctcct cgaacccctc tagcggctcg ccgccaacgc
     2041 gctgggtagg ggagtcgggg gtgacgagct cgggatgctc ggcctccatg ctctcgagct
     2101 cggcgtagag ggcgtcgtac tcggcgtcgg agatctctgg cgcgtcttct atgtggtagc
     2161 ggcggttgtg gtagcggacc tgctcgcgca gaccctcgat cctgctccgc acgtcaacgc
     2221 tctcggccac gcgaagatta tacgcgcggg tgcccgccgc cccaagagcg gcttataccc
     2281 gccggaccgg ccagccgagc gccctgccgt tctcgtccac gaaggagaag tccttgcctc
     2341 cggggtcaag ctcccggatc accgccacgg cgtagggata ggtgagggcc tgggtcagga
     2401 tcgcatcgcg cggcggctct ttcaacgcga gcctcacagt caccctgctc ccttcgaggc
     2461 gcgcggaccg cacggacatc gagtacccgc ctgtcggctt ctcgccccag tagacggcga
     2521 ggtacgtacc gctccccgag tcgggaacgt tcgcgccgat ctc
//
LOCUS       DASTUV010000441         5665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_659432, whole genome shotgun sequence.
ACCESSION   DASTUV010000441 DASTUV010000000
VERSION     DASTUV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659432"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFT03_08900"
     CDS             <1..471
                     /locus_tag="VFT03_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4848304.1"
                     /translation="VGNMGHFRLRQHRESEKMLEDFVGQVAEIAVLLVFVTLGINLPF
                     DALGKYLLGGLVVMVVFIFVARPVTVLACLLPDRRGRWTREEIIFLSWCRYTGVIPAA
                     VASILLARGVEGAEIAVSLVALAVVATLLLQATTAGWLARRLGLIEDPDPAAPT"
     gene            526..1509
                     /gene="moaA"
                     /locus_tag="VFT03_08905"
     CDS             526..1509
                     /gene="moaA"
                     /locus_tag="VFT03_08905"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU4848305.1"
                     /translation="MQLTDSHNRKMDYLRISVTDRCNFRCQYCMPEDIQFQDKSHILT
                     YEEMLLFAEACLQLGVTKVRVTGGEPLVRRGVVWFVSQLKELGFEDVTMTTNGFLLKD
                     NLEGLVEAGLDRINISLDTLQPEKFAFITRRDHFQKVWEAIMAALETPLSPVKINAVA
                     MRGINDDEIADMARLTLRYPMHVRFIELMPLNGDTDGSRFRSLFISGREILERAQKEL
                     GDLKPVEKVDPAAPADEFKFDGAPGTFGVITPVSEPFCARCSRLRLTADGKIHPCLLT
                     DLDVPVKDAIRSEDPIPAIHKVLWETARVKPAAGLTLPEESRNRTMSQIGG"
     gene            complement(1578..1913)
                     /locus_tag="VFT03_08910"
     CDS             complement(1578..1913)
                     /locus_tag="VFT03_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEU4848306.1"
                     /translation="MPSSGTLEARSPSIFSTVKPAPAPKEKTERSVAQEPPYKVILHN
                     DDYTPMEHVVEVLRKVIPRMSVRRAVDIMLEAHTKGKAVVTKTHKELAELYQEGLNNE
                     GLIASIEPD"
     gene            complement(1958..3118)
                     /locus_tag="VFT03_08915"
     CDS             complement(1958..3118)
                     /locus_tag="VFT03_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEU4848307.1"
                     /translation="MSGRAALVHDPALEAYGFGGDHPFNPLRIRLTLELCETLGLLQD
                     YPYIVSEPASEADLTAVHSLTYVRLVQEAGRGGGELWDLMHYGLGTGDNPVFPDMHEA
                     CALVVGAVLRASRMVMSGETDHAMCVSGGLHHAMRSRASGFCVYNDAGVAIARLKEEH
                     PGVRIAYLDTDAHHGDGVQWMFYDDPEVLTVSMHESGRYLFPGTGGVEERGRNGGVGY
                     SVNVPLEPYTDDSSWISCFETVVPEVLRAFEPDLILSQNGCDGHALDPLTHLSATTRL
                     YEHIPLRVHDLAHELCEGRWVATGGGGYDIWRVVPRAWTALWAAVSHQELPEKVPDDW
                     LASRGAQSPVALPRLMRDDPADYPVAERAAEIAYRNRRTTERVLENVMPLIR"
     gene            complement(3115..3786)
                     /locus_tag="VFT03_08920"
     CDS             complement(3115..3786)
                     /locus_tag="VFT03_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization AcuB family protein"
                     /protein_id="HEU4848308.1"
                     /translation="MQISGKAPMLLVKDSMSRQVVALPPQATAGEALALCRERRIRHM
                     PVLEDGRLVGIVSDRDLRSATPALGDPARAEALGRILISEVMASEVTTALPDDPIEEA
                     ANAMREKKIGCLPVVEDENLVGIISSSDVMEALVYLMGAHEPGNRLEVVMPDRPGTLA
                     GVAGIFGELGINIVSAVLGPRRDLSERGEPGRVAVFRVDTMDLREAVEILESAGYDVL
                     WPPEQ"
     gene            3857..4231
                     /locus_tag="VFT03_08925"
     CDS             3857..4231
                     /locus_tag="VFT03_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848309.1"
                     /translation="MSEQLNQFLAEKAGALVRENFTGVRAEWWWERRMGGGIEVCQEL
                     DPEAMSREVSERTGRDAEQVRRVIEKELGLEDMEPVVLTFEISGEATAEEAAKMLAER
                     SSVPEGLAAGLYRRIEETVGRN"
     gene            complement(5115..5489)
                     /locus_tag="VFT03_08930"
     CDS             complement(5115..5489)
                     /locus_tag="VFT03_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848310.1"
                     /translation="MFKRTARALVGGGRFVFGMGFLLAPSPILRIWWANGGASGPIAS
                     GLARGLGARDAALGLGILVAVLRRESSRGWLLAAASADVTDLVLTLANAYPFGRRTRL
                     LTLAGIALYVMLQLYLAFDQED"
BASE COUNT         1069 a         1847 c         1769 g          980 t
ORIGIN      
        1 gtaggtaaca tgggccattt caggctccgc cagcacaggg agagcgagaa gatgctcgaa
       61 gacttcgtgg gccaggttgc ggagatcgcc gtgctcctgg tattcgttac gctggggatc
      121 aacctgcctt tcgacgcgct cgggaagtat ctactcggcg ggctcgtggt gatggtggtc
      181 ttcatcttcg tcgcgcgccc ggtgaccgtg ctggcctgtc tcctccccga ccggaggggg
      241 cgctggacgc gcgaggagat tattttcctg agctggtgcc gttacacggg ggtcatcccc
      301 gctgcagtag cgagcatact cctagcccgc ggggtcgagg gcgcggagat cgcggtctcg
      361 ctcgtcgcgc tcgccgtggt cgcgaccctc ctcttgcagg ccactaccgc tggctggctg
      421 gcacgcaggc tcggcctgat cgaggatcct gaccccgccg ctcccacgta ggcaccagcc
      481 cgaaaagaga tacaatgggg ggtgggaacc gcgaggaagg acacggtgca gcttaccgac
      541 agccacaacc ggaagatgga ctacctgcgc atctcggtca ccgaccggtg taacttccgc
      601 tgccagtatt gcatgcccga ggacatccag ttccaggaca agagccacat cctcacctac
      661 gaggagatgc tcctcttcgc cgaagcctgc ctccagcttg gcgtgacgaa ggtgcgcgtg
      721 acaggcgggg agcccctggt gcgccgcggg gtcgtctggt tcgtaagcca gctcaaagag
      781 ctcggcttcg aagacgtcac catgactacc aacggcttcc tcctcaaaga taacctcgaa
      841 ggcctcgtcg aggctggcct cgaccggatc aacatttccc tcgacaccct ccaaccggag
      901 aaattcgcct tcatcacgcg ccgcgaccac ttccagaagg tctgggaggc gatcatggcg
      961 gcgctcgaga cgcccctttc gcccgtcaag ataaacgccg tggcgatgcg cggcatcaac
     1021 gacgacgaga tagcggatat ggccaggctc accctgcgct acccaatgca cgtccgcttc
     1081 atcgagctca tgcccctcaa cggcgacacc gacggctcgc gcttccgctc cctcttcatc
     1141 tccggacgcg agatcctcga gagggcgcaa aaggagctcg gagacttgaa gcccgtcgag
     1201 aaagtagacc cggcggctcc ggccgacgag ttcaagttcg acggcgcgcc aggtaccttc
     1261 ggtgtgatca cacccgtctc ggagcccttc tgtgcgcgct gctcgcgcct caggctcacc
     1321 gcggacggca agatccatcc ctgcctcctc accgacctcg acgtccccgt caaggacgcg
     1381 atacgctccg aagacccgat ccccgccatc cacaaagtgc tgtgggagac cgcgagggtc
     1441 aagccagccg cgggtctcac ccttccagaa gaatcgcgca accgcacgat gagccagatc
     1501 gggggctgag ggccctgttc ttcgcgccta gaaccggctg ttaaccggat gcggtactcg
     1561 acccttttgc gcgctggcta gtccggttct atggaggcga tgaggccctc gttgttgagg
     1621 ccttcctggt agagctcggc gagctccttg tgggtcttgg tgacgacggc tttgcccttg
     1681 gtgtgggcct cgagcattat gtccaccgcc cgccgcaccg acatgcgggg tatcaccttc
     1741 cgcagcactt cgacgacgtg ctccatcggg gtgtagtcgt cgttgtgcag gatcaccttg
     1801 tacggtggtt cctgcgcgac gctgcgctcg gtcttctcct tgggagccgg cgcaggctta
     1861 accgtggaga agatcgacgg gctacgcgct tcgagtgtgc cggatgaggg catcttttcc
     1921 accggacctg gagactacca caggcgcggc ccagagctca acggatcaga ggcatgacgt
     1981 tctcgagcac ccgctcggtg gtgcgacggt tgcggtacgc tatctccgcg gcgcgctccg
     2041 cgacgggata atccgccggg tcgtcgcgca tcaggcgtgg tagagctacc gggctctgag
     2101 cgccccgcga cgcgagccag tcgtccggca ctttctccgg cagttcctgg tgtgagacgg
     2161 cggcccagag ggccgtccag gccctcggga cgacgcgcca gatatcgtaa cctccgcctc
     2221 cggtggcgac ccagcggccc tcgcacagct cgtgggcgag gtcgtggacc ctgaggggta
     2281 tgtgctcgta gagacgggtc gtggccgaga ggtgggtcag ggggtcgagt gcgtggccgt
     2341 cgcaaccgtt ctgagagaga atcaggtcgg gctcgaaggc gcgaagcacc tcggggacga
     2401 cggtctcgaa gcaggagatc caggacgagt cgtccgtgta cggctccaac ggcacgttga
     2461 ccgagtatcc caccccgccg ttgcgtccgc gttcctcgac gccgccggtc cccgggaaga
     2521 gatagcggcc ggactcgtgc atggagacgg tgaggacttc cgggtcgtca tagaacatcc
     2581 actgcacccc gtctccgtgg tgagcgtcgg tatcgaggta ggctatgcgg acgcctgggt
     2641 gttcctcctt gaggcgcgct atggctacgc ccgcgtcgtt gtagacgcag aaaccgctag
     2701 ccctcgagcg catggcgtgg tgcaggccgc ctgagacgca catcgcgtgg tccgtctccc
     2761 cgctcatgac catccggctc gcccggagca cagcgccgac tacgagggcg cacgcttcgt
     2821 gcatgtcagg gaagaccggg ttgtcccccg taccgaggcc gtagtgcatg aggtcccaca
     2881 gctctccccc accccgccct gcctcctgca ccaggcgcac gtaggtcagg ctatggacgg
     2941 ccgtgaggtc cgcttcggag gccggctccg agacgatgta agggtagtcc tgaaggagac
     3001 ccagcgtctc gcagagctcg agggtgagcc ggatgcgcag cgggttgaag ggatggtccc
     3061 ctccgaaccc gtaggcttcg agggccgggt cgtggactag ggcggcccgg ccgctcactg
     3121 ttctggcggc cagaggacat cgtagcccgc gctctcgaga atctccacgg cctccctgag
     3181 atccatggtg tcaacgcgga agaccgcgac gcgtccaggc tcccctcttt cggacaggtc
     3241 tcgccgcggg cccaacacgg cgctcacaat gttgatcccg agctcaccga agatccccgc
     3301 caccccggcc agggttccgg gacggtccgg catgacgacc tcgaggcggt tgccgggctc
     3361 gtgcgccccc atgaggtaga cgagcgcctc catcacgtca gaggaagaaa ttatgccgac
     3421 cagattttcg tcctcgacga cagggaggca gccgatcttc ttctctcgca tcgcgttcgc
     3481 cgcctcttct atggggtcgt ccgggagggc ggtcgtcacc tcactcgcca tgacttcgga
     3541 gatgaggatc ctgcccagag cctccgcgcg ggcggggtcc ccgagggccg gggtggcgga
     3601 gcgcaggtcc ctgtcggaga cgattccgac gagcctcccg tcttccaata ccggcatgtg
     3661 ccttatgcgc ctctccctgc acagagcgag cgcctccccc gccgtcgcct gcggcgggag
     3721 cgccacgacc tgacgggaca tcgagtcctt tacgagcagc atgggggcct ttcccgagat
     3781 ctgcaactcc ttccctgttc atattgtata ggagccggtg cctctgggca ctctcccaag
     3841 ctggtataaa gagaccatgt ccgagcagct gaaccagttc ctggcggaga aggccggcgc
     3901 gctagtgcgc gagaacttta ccggagtccg cgccgagtgg tggtgggagc gaaggatggg
     3961 cggcggaatc gaggtctgcc aggagctcga cccggaggcc atgagccgcg aggtctccga
     4021 gcggacgggt agggacgcag aacaggtccg gcgcgtcata gaaaaggagc tcggcctcga
     4081 ggacatggag cctgtcgtgc tcaccttcga gatatcgggc gaagccaccg cagaagaagc
     4141 ggcgaagatg ctcgccgaac gctcgtcggt gcccgagggc ctcgccgctg gactctaccg
     4201 caggatcgaa gagaccgtcg ggcgaaacta gcagcaggcc aacgagatat accttccagg
     4261 cgtatctctc cgcgaagcga aatagcccgg ctgttcttta acctcgtctc aatgccggtg
     4321 ggagcggagc tcagcccgga gaagtgagca gcgcaggata attcggtggc ttggccagct
     4381 gcagcctaac tctcacgggg tcgaagagcc acagggtccg acgtttctgg caatctagcg
     4441 ctcttcgtgc gccgaacgtc ggagaagact ctcctacctc aagggtgatg cggcgcggtc
     4501 ggtgacgccg cgtccgcggc cgtattgcca aacccctttc gctcgaggcg cccggaggga
     4561 gcaagccgct taccgcactc gcactcgaag gagatgccgc ccgataacca caccgagatc
     4621 ggcccgtcta gcaccatcct ctctccgcaa tcctcgcata caagaatgcc acctctagaa
     4681 ggatcgttca cacgccttcc cccctcctcc atcatgccga cgctcgcaaa acttcgcgca
     4741 cccgagggat cagcacaacg tcaacctcct cgaagccgcc ttactccata gcatcgctcc
     4801 gagcctacac gtcgctcaca gccgccggca aggtctagcg acctcgacct gtacctcagc
     4861 gtgaactcgt aatcaagtcg aagttcccgt acacctctac ctcgatcgga gcttgcgtcg
     4921 cgccagcgac ctactgaatc cgcgtactta taggcctgag aagaaacgag cctcgacctc
     4981 tggtccaatc atgaagatgt ctcacaatac ctgcaagcgg caggtgaggt tcatccgcgt
     5041 ggggtccact accgcgtcgt caaaggcagc tcggttgcta gccgctagtt cgatacgaga
     5101 ggctggggag gactctagtc ttcctgatcg aaggcgagat atagctgcag catcacgtac
     5161 agggctatac cagcgagggt cagaagccta gtacgacggc caaaagggta ggcattagcc
     5221 aacgtcagaa ccaggtcggt gacgtcggcg ctggcggcag ccaggagcca gcctcgagag
     5281 gactcgcgcc gcaggacggc aaccaggatt cccagcccca gggcggcgtc acgagccccc
     5341 agcccgcgag caaggcccga agcgatgggt ccggatgctc cgccgttcgc ccaccagatc
     5401 cgcagtatcg gggaaggagc gagcaggaat cccatgccaa agacgaaccg gccgcctccc
     5461 accagagccc ttgcggttcg cttgaacacg cccacaaata tacagtaccg accacccgga
     5521 cgaaatattc accttcgagc ggccaaccga gccgagaagt gcaaggccca aaagctctcc
     5581 caatgtgcta tgaacacgga cgttactgcc caggagacca cggctcgaat agtggtagga
     5641 atagaggcag gagaaatgaa cgttt
//
LOCUS       DASTUV010000442         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1420860, whole genome shotgun sequence.
ACCESSION   DASTUV010000442 DASTUV010000000
VERSION     DASTUV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420860"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VFT03_08935"
     CDS             <1..1188
                     /locus_tag="VFT03_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU4848311.1"
                     /translation="KPGDRLPFGQEMPEPAPVAEILRRVHAMPLPEEVVGRDLRELPG
                     GVSNVTHGEAVRRGVGYAVGFKNIGFSAGFDDYSTARVTLSVQDGEPLAQVHTAAAEV
                     GQGLITIEAQIARTELGVERVAVLPADTKVGSAGSTSASRQTYVTGAAVKQACEALRE
                     RVFEKAREEFGKEDFAIEGANVVSDGETVVSLAELLGGEEIEETVEFHHRETEPLDER
                     GQGDAHLQFAFAAHRAVVEVDTELGLVRVVEVATAQDVGKAMNPQALEGQIEGGIAQG
                     LGLALLEEIQIKEGRIQNASFTDYLIPTILDMPAVRMDVLELADPEAPYGLKGVGEPP
                     TIASTPAIVAALRDATGRELTRVPVKPEQIVGIAEKPPGEPPQPGSRGYSGIGRLDDR
                     EEH"
     gene            1188..1472
                     /locus_tag="VFT03_08940"
     CDS             1188..1472
                     /locus_tag="VFT03_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU4848312.1"
                     /translation="MPAYERAELSAELKVLPSPEAEKSPREQVEEAKRAASGSGLARE
                     GGPETTVLAGGRKEVLEAAMKTVEAALDAGARAVEVKVEAEGESERFGGG"
     gene            1499..2035
                     /gene="uraD"
                     /locus_tag="VFT03_08945"
     CDS             1499..2035
                     /gene="uraD"
                     /locus_tag="VFT03_08945"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEU4848313.1"
                     /translation="MSERATIEEVNGLDREEFVSRFGGLYEDSPWVAEAAWRERPFAS
                     FAELHDAFVRALREAHREQQLALIRAHPDLAGRAAIAGELTAESAREQTSAGLDRLTP
                     GEYEDFHRLNSAYRARFGFPFVVCVRDHGKETILAAAAARLGHSRPEEIETALGEIGR
                     IAYLRLRDLVEPREGEKQ"
BASE COUNT          361 a          626 c          807 g          285 t
ORIGIN      
        1 aaacccggag atagattgcc gttcgggcag gagatgccgg agcccgcccc ggtggccgag
       61 atcctgaggc gcgtacacgc catgcccctg ccggaggagg tggtggggcg cgatctgcgc
      121 gagctgcccg gcggcgtctc caacgtgacg cacggcgagg ccgtgaggcg cggcgtgggc
      181 tacgccgtag gcttcaagaa cataggcttc tcggctggct tcgacgacta ttcgacggca
      241 cgcgtcactc tttccgttca agacggcgag ccgctggctc aggtccacac ggcggcggcg
      301 gaggtggggc aggggctcat cacgatagag gcccagatcg cgcgcaccga gctgggcgtc
      361 gagagggtcg ccgtactccc cgcggacacg aaggtgggtt cagccggctc cacctcggcc
      421 tcgcgccaga cctacgtgac cggcgccgcg gtcaagcagg cctgcgaagc cttgcgcgag
      481 cgcgtcttcg agaaggcgcg cgaggagttc gggaaggaag acttcgccat agagggagcc
      541 aacgtcgtct cggacggcga gacggtcgtg tcgctcgcgg agcttctcgg cggcgaggag
      601 atcgaggaga ctgtcgagtt ccaccacagg gagaccgagc cgctcgatga gaggggacag
      661 ggcgacgctc acctgcagtt cgcgttcgcg gcgcacaggg cggtcgtgga ggtcgacacg
      721 gagctcggtc tggtccgggt agtcgaggtc gcgacggcgc aggacgtggg caaggcgatg
      781 aacccgcagg cgctggaggg tcagatcgag ggtggcatcg cgcaggggct cgggctcgcc
      841 ttgctcgagg agatacagat caaagagggc aggatccaga acgcctcctt caccgactac
      901 ctgatcccca cgattctaga catgccggcc gtgaggatgg atgtcctgga gctggccgac
      961 ccagaagctc cctacggcct gaaaggcgtg ggcgagccgc cgacgattgc ctcgaccccc
     1021 gcgatagtcg cggccctgcg cgacgccaca ggccgggagc tgacgcgcgt ccccgtgaaa
     1081 ccggagcaga tcgtgggaat cgccgagaag ccgccgggcg agcccccgca accgggcagc
     1141 cgcgggtact ctggcatcgg acgcctcgac gatagggagg agcactagtg cccgcatacg
     1201 agagggccga gctgagcgcc gagctgaagg tcctcccatc cccggaggcc gagaagtccc
     1261 cgcgggagca ggtcgaggag gcgaagcggg cggcgagcgg atcgggtctg gcgcgcgagg
     1321 gcgggccgga gaccaccgtg ctcgccggcg ggcgtaaaga ggtgctggag gctgcgatga
     1381 agaccgtgga ggcggcgctc gacgccgggg cgcgcgccgt cgaggtgaag gtcgaagctg
     1441 aaggggagtc cgagaggttc ggcggaggtt agccggaccc cgcaggtagg tgggcgttat
     1501 gtccgagagg gccacgatag aagaggtgaa cgggctcgac cgggaggagt tcgtctccag
     1561 gttcggcggc ctctatgaag actcgccctg ggtcgcggaa gcggcctggc gcgagcgtcc
     1621 gttcgccagc ttcgcggagc tgcacgacgc gttcgtgagg gcgttgcggg aggcgcatcg
     1681 cgagcagcag ttggccctca tccgggctca cccggacctg gcggggaggg cggcgatcgc
     1741 gggcgagttg acggccgagt ccgcgcgcga gcagacctcg gcggggctgg acaggctgac
     1801 gcccggtgag tacgaggact tccaccggct gaacagtgcg taccgggcca ggtttggctt
     1861 ccctttcgtg gtgtgcgtca gggatcacgg caaggagacg atcctcgccg ctgctgcggc
     1921 ccggctcggc cactcgcggc ccgaggagat cgaaacggct ttaggcgaga tcggcaggat
     1981 cgcctacctc aggctgcggg acctcgtcga accgcgtgaa ggggagaagc aatgagctat
     2041 ccgcgggtgg tgcctggcct gcggagcgag ggacgagga
//
LOCUS       DASTUV010000443         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1009257, whole genome shotgun sequence.
ACCESSION   DASTUV010000443 DASTUV010000000
VERSION     DASTUV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009257"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..152
                     /locus_tag="VFT03_08950"
     CDS             66..152
                     /locus_tag="VFT03_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848314.1"
                     /translation="MDFVAGMTDRYALATYRRLFLPRGEIFA"
     gene            complement(198..2993)
                     /gene="ppc"
                     /locus_tag="VFT03_08955"
     CDS             complement(198..2993)
                     /gene="ppc"
                     /locus_tag="VFT03_08955"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU4848315.1"
                     /translation="MAPQAEYADLPPGVGFEPKDEPLRRDINLLGRVLGQVLIEQEGE
                     GLFDAEEEVRLLCKRLRFDYDPELDERLRRKIEGMDAEQLQRIVRAFSVYFQLVNIAE
                     RYHRVRRRRQYESSPSNPPQRASLASALSRLESEGVEADTLQRALDGMSVELVLTAHP
                     TEALRRSIRRRHLRIGEVLETLESPQLTWKERRRLEERLAEEITVLWQTDELRVRRPQ
                     VEHEIERTLGFFENPLISATLEVYRDFEDELARRFPGNTPTLGRVLSFGSWVGGDQDG
                     NPFVRPDTLSTALSLHRELILKRHRASTLALAEHMSQSRKLAAVSEELAASVERDEGL
                     MPEVAERHRDDDPNEIYHRKLLLVAERLRRTLEDSGAQAAYSGVSDFLNDLLVIRRSL
                     VDNGDERIAEGGLRDLIRQAEVFGFHLAKLDVRQESSRIVEAAAELVATGTGEDLLGM
                     SEEARAALLRRLIADPESLPDNPKDISEASIEVLETFERIRQATDAFSEPPIETFILS
                     MTHQASDVLSVQLLARRAGLLEADERGRCTRSLLHITPLFETIEDLERAPDVLRRLLE
                     DPFYRSSVARGGNLQEIMLGYSDSGKDAGYVTSNWTLYKAQGLLASLAREHAVELRLF
                     HGRGGSAGRGGGPSYQAIMAQPPGTLDGKIRITEQGEVISFKYSIRGLARRNLDTVLA
                     AVLEASADDHPKEPDPRWVEVMNELSATSCEAYRALVYEDGDFLAFFSEASPIKELSL
                     LNMGSRPASRVEAPEIESLRAIPWVFAWTQNRFLLPSWYGAGTALGTYSSSEEGLAVL
                     REMYEQWPFFRTLVDFMQMTLAKSDLRIAETYTSLVSNSAARERLWNRISAEHAACVE
                     ALLKITGHESLLDDSPVLQRSIRLRNPYVDPLSYVQVSLLRRLRALPEDSPKRQATLN
                     TLLLTISGISSGMLNTG"
     gene            3113..>4782
                     /gene="dnaG"
                     /locus_tag="VFT03_08960"
     CDS             3113..>4782
                     /gene="dnaG"
                     /locus_tag="VFT03_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU4848316.1"
                     /translation="MRISDRSIEQVREHANIVEVASEFTALRRQGTRFVGLCPYPDHS
                     EKTPSFSVHAERNFYHCFGCSKGGDAIKLVMDLKAFSFVDAVSYLAERSGVELEFEGG
                     GNLDAARERNLRRRNIHKALAAAAVYYHKYLLKARSSEAGRARRYLKGRGLELSTIEE
                     FRLGFAPPRGIGGFAGAAAKLGIERKVLDAAGLLSAGGRERFAGRITFPISDRRGRIV
                     GFGARSLSDDHPKYLNSPETEIFNKRDLLYGFPQVSEAMRKERAAIIVEGYTDVLMLY
                     QSGIRNAVATLGTATTPSQLRTLSGYADKIYMLFDPDTAGEKALERADATVRELERTD
                     DPDTVATATRLKLDLRVLRLTQDPADWLLDHSPEEFAEMLSGAVTLLEYIFRRKVERA
                     RGASAAERSRMMSEVRGLISRIDDPVFYREALRVAAEAMGVSSQELESGRRQRVVRAT
                     PSRHAVSAPLEQAGQLVLALLLAYPDLTAKPLERGARVPPLPEPFKLDARDFAIEPQS
                     SVFHLLRKHPGESLESILADESARPLMDQLVALGAEAEELRRNGLYSS"
BASE COUNT          872 a         1517 c         1498 g          895 t
ORIGIN      
        1 tcgagtatta cctcgagcat ggcggggagc gtgccacgag cgacccggac cagataatcg
       61 agactgtgga cttcgtagct ggcatgaccg accgttatgc cctcgctacg tacaggaggc
      121 tcttcctgcc gcgaggcgag atcttcgcgt agcaggggtg gtccgcgaac cacccttaca
      181 cgaaccgccc ctacgcgtta acccgtgttc agcatcccgg atgagatgcc ggagatggtg
      241 agcaggaggg tgttcagcgt cgcctggcgc ttgggggagt cttcgggcag agcccgcagt
      301 ctccggagca ggctcacctg tacgtaggag agaggatcga cgtacgggtt gcgcaggcgt
      361 atggacctct gcaacaccgg gctgtcgtcg aggaggctct cgtggcctgt gatcttgagc
      421 agcgcctcca cacaggcggc gtgctccgcg gaaatcctat tccacaggcg ttctctggct
      481 gcgctattcg agacgagcga ggtataggtc tcggctatac ggaggtcgct cttcgccagg
      541 gtcatctgca taaagtcgac gagagttcga aagaaaggcc actgctcgta catctcgcgc
      601 aggacggcga gcccctcctc gctgctcgag taggtgccca gagcggtgcc tgccccgtac
      661 caggagggta gcaagaagcg gttctgggtc caggcgaaga cccatggtat ggctcgcaag
      721 ctctcgatct ctggggcctc gaccctactg gcgggacggc tccccatgtt gagcagggag
      781 agctctttta tgggcgatgc ttccgagaag aaggccagga agtccccgtc ttcgtagacc
      841 agggcccgat aggcttcaca ggaggtcgcc gacagttcgt tcatgacctc gacccagcgc
      901 gggtcgggct ccttgggatg gtcgtctgcg ctcgcttcga gaaccgcggc gagcacagta
      961 tcgaggttac gccgtgccag gccgcggatg ctgtacttga aggagatgac ctctccctgc
     1021 tctgtaatcc gaattttgcc gtccagggtg cctggaggtt gggccatgat cgcctgatag
     1081 ctgggaccgc cgccccgccc ggcgctgcct ccgcgtccgt ggaagagccg cagctctaca
     1141 gcgtgctcgc gggcgagcga agcgaggagt ccctgcgcct tgtacagggt ccagttgctc
     1201 gtcacatacc cggcgtcctt gccggaatcg ctatagccga gcatgatctc ttgcaggttc
     1261 ccgccgcggg ccacagacga tcggtagaaa gggtcctcca gcaggcgtct cagcacgtcg
     1321 ggcgcccgct ccaggtcctc aatggtctcg aagagaggag tgatgtgcag gaggctcctc
     1381 gtacaacggc cgcgctcgtc cgcttccagc aaccccgccc ggcgcgcgag cagttggacg
     1441 cttaggacgt cgctcgcctg gtgggtcatg ctcaggataa aggtttctat aggcggctcg
     1501 gagaaagcat ccgtcgcctg ccggatgcgc tcgaaggttt cgagcacctc aatggaggcc
     1561 tcggagatgt ccttcgggtt atcggggagt gactcggggt cggcgatcag gcgccgcaac
     1621 agcgccgccc gggcttcttc ggacatgccc aacaggtcct cgcccgtacc tgtggctacc
     1681 agctccgcgg ccgcctcaac tatcctggag ctctcctggc gaacgtcgag cttcgcgagg
     1741 tggaagccga atacctccgc ctggcggatc agatcccgga gccccccctc ggcgatccgc
     1801 tcgtcgccat tgtccaccag gctgcggcga atgacgagca ggtcgttcag gaaatccgac
     1861 acgccagaat aggccgcctg ggctcctgaa tcctcgagtg tgcgcctcaa acgctcggct
     1921 acgagcaaca acttgcggtg gtatatctcg ttgggatcgt cgtcccggtg acgctccgct
     1981 acctcgggca taagcccttc gtcgcgctct acggacgccg ccagctcttc cgaaaccgcg
     2041 gctagcttcc tagactggct catgtgctcg gccagagcca gggtcgaggc acgatggcgc
     2101 ttcaggatca actcgcggtg gagggagagg gccgtgctca aggtgtcggg ccgcacgaac
     2161 gggtttccat cctgatcccc gccgacccag gagccgaagc tcagaacccg cccgagggtc
     2221 ggggtatttc ccggaaaacg gcgggccagc tcgtcctcga aatcccggta cacttcgagg
     2281 gtcgcggaga tcaaggggtt ctcgaagaac cccagcgtac gctctatctc gtgctcgacc
     2341 tggggccggc ggacccttag ctcgtcggtc tgccacagga cggtgatctc ctccgcgagc
     2401 ctctcctcca ggcggcgcct ttccttccag gtgagttgcg gggattcgag ggtctcgagc
     2461 acctcgccta tcctcaggtg cctgcgccgt atgctccgcc tgagggcctc cgtcggatgg
     2521 gccgtcaaca caagctccac gctcatgccg tccaacgccc gctgcaacgt atcggcctcc
     2581 acaccctctg attcgaggcg cgagagagcg ctcgccagcg aggcccgctg cggcggattg
     2641 ctcggggacg actcgtactg gcgccgccgc ctgaccctgt ggtatcgctc cgcgatgttg
     2701 acgagctgga agtaaaccga gaaggcccga acgatcctct ggagttgctc cgcgtccata
     2761 ccttcgatct tccgcctgag cctctcgtcc agctcggggt cgtaatcgaa gcgcagccgc
     2821 ttgcaaagga gccgaacctc ctcctcggcg tcgaagagtc cttcaccctc ctgctcgatc
     2881 agaacctggc ccagaaccct tccgagcagg tttatgtcac gccgaagcgg ctcgtccttg
     2941 ggctcgaaac cgaccccggg gggcaggtcc gcgtactccg cctgcggcgc catcgtggat
     3001 cgctcgcgga tattcatcgc attagagtac cattaccctg acttggatca cgagggagca
     3061 ggggaatgtt tgtactagga tatattcgca agaggactat agaggcgagg gcttgaggat
     3121 ctcagaccgc agcatagagc aggtcaggga gcacgcgaac atagtcgagg tcgcctccga
     3181 gttcaccgcc ttacgccgcc aaggaacgcg cttcgtcggt ctctgccctt accccgatca
     3241 ctccgagaag accccctctt tcagtgtgca cgcagagcgg aacttttatc actgtttcgg
     3301 ttgtagtaaa ggcggtgacg caatcaagct agtcatggat ctcaaggcgt tctccttcgt
     3361 ggacgccgtc tcctacctgg ccgagcgctc cggcgtcgaa ctcgagttcg aaggcggcgg
     3421 aaaccttgac gccgcaaggg aacgtaacct ccgccgccgc aatatccaca aagcactcgc
     3481 cgccgcagcc gtctattacc acaagtacct gctgaaggcg cgctcttcag aagcggggcg
     3541 cgcccgccgt tatctcaaag gccgcggcct cgagctttct actatagaag aatttcgttt
     3601 gggctttgcc ccgcctcggg gtattggggg gtttgcggga gctgcggcga agctgggcat
     3661 cgagcggaag gtgcttgatg cggctgggct gctatcggct gggggaaggg aaaggtttgc
     3721 gggcaggatc accttcccca tctcggacag gcgtggcagg atcgtgggct tcggggcgcg
     3781 ctcgctcagc gacgaccacc ccaagtacct caattcgccc gagacggaga tcttcaacaa
     3841 gcgggacctg ctctacggtt tcccgcaggt ctccgaggcg atgcgcaaag agcgcgcggc
     3901 catcatcgtc gagggctata ccgacgtact gatgctctat cagtccggca tcaggaacgc
     3961 cgtggcaacc ttgggaacgg ctaccactcc gagtcagcta cggacgctca gcggttacgc
     4021 tgacaagatc tacatgctct tcgatccgga cactgcgggg gagaaggccc tggaacgagc
     4081 cgacgcgaca gtacgggagc tcgagcgaac cgacgatccg gacacggtag cgacagcgac
     4141 caggctcaag ctggacctgc gcgtgctgcg tctgacccag gatccggccg actggctgct
     4201 agatcactcg ccggaggagt tcgcggagat gctttcgggc gcggttacgt tgctcgagta
     4261 tattttccgt cgcaaggtcg agcgggctcg cggagccagc gccgccgagc gttcccggat
     4321 gatgtcggaa gtccgtggcc tcataagccg cattgacgat cccgtcttct acagggaagc
     4381 cctgcgtgtg gccgccgaag ccatgggtgt aagctcgcag gagctcgaga gcggaaggag
     4441 acagcgggtc gtgagggcta caccttcccg gcacgccgtt tcagctccct tggagcaggc
     4501 cggacagctg gtgcttgcct tgctcctggc ctacccggat ctcaccgcga agccactgga
     4561 gcgaggcgcg cgagtccctc cgctgcccga gcccttcaaa ctcgatgcca gagactttgc
     4621 gattgagccc caatccagcg tcttccacct gctgcggaag caccccggag aaagcctcga
     4681 atccatcctc gccgacgaaa gtgcccggcc tctgatggac cagcttgtcg ccctcggcgc
     4741 cgaagcagag gagttacgcc gaaacggtct ttactcctca ga
//
LOCUS       DASTUV010000444         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1069477, whole genome shotgun sequence.
ACCESSION   DASTUV010000444 DASTUV010000000
VERSION     DASTUV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069477"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFT03_08965"
     CDS             <1..1029
                     /locus_tag="VFT03_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4848317.1"
                     /translation="GNLSETTTARETPGAGTRERGLFGRLLIGLVRVREASVFIVLVG
                     MVIYFQAVNPAFLSEQNILSLAQFAVPYVVISAALCMLLICGEIDLSVGQSFAFAPIV
                     MYLAYEQLYLYLPLAVIVGLLAVALVGLVNGLITVYLGVSSLITTLGMFFLLAGLNVT
                     LTGGFPATPPETNFVTQALGEYAWSGTIWAIAIVAILQLVLSRTRWGLHTYATGGNLL
                     GAREAGVSIARIKIGNFILCGVLGGFVGIIEAFRISSIDPLAGGPDIVLLCIAAAVIG
                     GTALLGGVGTATGAFLGALVLVILRNGFTLQGISAYTFNIILGVAILVAMILNVYVAR
                     LRGAGSGE"
     gene            1029..1808
                     /locus_tag="VFT03_08970"
     CDS             1029..1808
                     /locus_tag="VFT03_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848318.1"
                     /translation="MQNDNGSDFLRVEGVVKNFGAISVLKGVDMHVKRGEVLGLIGDN
                     GAGKSTLLKTITGFHRPDGGRILIEGEEVNLRSVSQARALGIQTVYQDLALVNELSVF
                     HNMFLNSEVTVGPFLNNGKMKERTQRYLDDMGVRIPSIDTEVARLSGGQRQAIAVARA
                     VYSDAKMLLLDEPLAAMGAKEGALILDLIQRLKDERGIPMILIVHNYAQVFDVCDRVN
                     LLRNGRIEYDKPVAETSVEELTELVVSEYRKARETGNGQSR"
     gene            complement(1836..>2730)
                     /locus_tag="VFT03_08975"
     CDS             complement(1836..>2730)
                     /locus_tag="VFT03_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4848319.1"
                     /translation="RVEILPGIHRIESDLGVRFMCQYLLTGEDHTVLVDTGLAGTPEE
                     VIIPYLEGVGLSLEDIDEVIISHADVDHCGGNRALKEMHPALWFTCGEPDRAYVENNE
                     VMTAEIYRWSEPYGFGPDEDSLEWIRRELGGDSPVDAGLRGGETMRLGPERRIEILHL
                     PGHTPGHLGIWDPKNGAAIIIDAALETGIYDREGNRLIPPRYFDAAAYQATIRKLQTL
                     RPEHLLTAHYPPMQGDEALDFLDRSLQFTQQLHGVVSEGLQSGTTDLWELTRRAVAQL
                     GPYPEFGHELGAGVRAHLARM"
BASE COUNT          438 a          897 c          899 g          496 t
ORIGIN      
        1 gggaacttga gcgagaccac caccgcgcgg gagacgccgg gggccgggac gcgggagcgc
       61 gggctcttcg gtcggctgct gatcggcctc gttcgtgtgc gggaggcgag cgtcttcatc
      121 gtcctcgtgg ggatggtgat ctacttccag gcggtgaacc ccgcgttcct ctcggagcag
      181 aacatcctct cgctcgccca gttcgccgtt ccctacgtgg tcatctcggc ggcgctgtgc
      241 atgctgctca tttgcggcga gatagacctc tcggtcggcc agtcgttcgc gttcgcgccc
      301 atcgtcatgt acctcgccta cgagcagctc tatctgtatc tgccgctcgc ggtcattgtg
      361 gggctgctcg ccgtcgcgct ggtcgggctc gtgaacggcc tgatcacggt ctacctcggc
      421 gtgtcgtcgc tgatcacgac gctggggatg ttcttcctgc tcgcgggcct gaacgtcacg
      481 ctcactggcg gcttcccggc gaccccgccg gagacgaact tcgtcaccca ggccctggga
      541 gagtacgcct ggtccgggac catctgggcc atagcaatcg tggccatcct ccagctcgtt
      601 ctgtctcgca cccggtgggg cctccacacc tacgcgacgg gcggcaacct gctcggggcg
      661 agggaggcgg gtgtgagcat cgcgcgcatc aagatcggca acttcatact ctgcggcgtg
      721 ctcggggggt tcgtcgggat catcgaggcc ttccgtataa gctccataga ccctctcgca
      781 ggcggtcccg acatagtgct cctgtgcatc gccgcggcgg tcataggggg gacggcgctg
      841 ctgggcgggg tcgggaccgc gaccggtgcg ttcctcgggg cgctggtgct cgtcatcctc
      901 cgcaacggct tcaccttgca ggggataagc gcctacacct tcaacatcat cctcggcgtc
      961 gccatcctcg tcgccatgat cctcaacgtc tacgtcgcca ggctgcgcgg cgcagggagt
     1021 ggtgagtgat gcagaacgat aacggctccg acttcctacg cgtagaaggc gtggtcaaga
     1081 acttcggcgc gatctcggtc ctcaagggcg tggacatgca cgtcaagagg ggggaggtgc
     1141 tcggcctcat cggcgacaac ggggccggaa agtccaccct gctcaagacc atcaccggct
     1201 tccaccgccc ggacgggggc agaatcctga tcgagggcga ggaggtcaac ctccgctccg
     1261 tctcccaggc gcgcgctctg ggcatccaga ccgtctacca ggacctcgcg ctcgtaaacg
     1321 aattgtcggt cttccacaac atgttcctga actccgaggt gaccgtgggg ccgttcctca
     1381 acaacggcaa gatgaaggag cgcacgcaac gctacctcga cgacatggga gtcaggatcc
     1441 cctccataga cacagaggtc gcccgcctct cgggcggcca gcgccaggcg atagccgtgg
     1501 cgcgcgccgt ctactcggac gcgaagatgc tcctcctcga cgagccgcta gcggcgatgg
     1561 gagccaagga gggcgcgctg atactcgacc tcattcagcg cctcaaggac gagcggggca
     1621 tacccatgat cctcatcgtc cacaactacg cccaggtctt cgacgtctgc gacagggtga
     1681 acctgctcag gaacgggcgc atagagtacg acaagcccgt cgccgagacc tcggtagagg
     1741 agctaaccga gctggtcgtc tccgaatacc gcaaggcccg cgagaccggg aacggccaga
     1801 gcaggtagcg ccggaacggt actccaggca tcctcttaca tcctggccag gtgcgccctg
     1861 acgccggccc cgagctcgtg accgaactca gggtatgggc cgagctgcgc tacggcgcgc
     1921 cgggtcagct cccagaggtc ggtggttcca ctctgcagcc cctcgctcac cacgccgtgg
     1981 agctgttgcg tgaactgtag ggagcggtcg aggaagtcga gtgcttcgtc cccttgcatc
     2041 ggcgggtagt gggctgtgag gaggtgttcg gggcggaggg tctggagctt gcggatcgta
     2101 gcctggtaag cggcggcgtc gaagtagcgc ggggggatca gacggttccc ctcgcggtcg
     2161 tagatgccgg tctcgagcgc cgcgtctatg atgatcgccg ccccgttctt cgggtcccag
     2221 atgccgaggt ggcccggcgt gtggccaggg aggtgcagta tctcgatgcg cctctcgggc
     2281 cccagcctca tagtctcccc gccgcgcaac ccggcgtcca cgggggagtc gccgcccagc
     2341 tccctgcgga tccactctag agagtcctca tccgggccga agccgtaggg ctcggaccag
     2401 cggtagatct ccgcggtcat cacctcgttg ttctcgacgt aggctctgtc tggctcgccg
     2461 cacgtgaacc acagggcggg gtgcatctcc ttgagggcgc ggttgccgcc gcagtggtcc
     2521 acgtcggcgt ggctgatgat gacctcgtcg atgtcctcca gggagagccc gaccccctcc
     2581 aggtagggga tgatgacctc ctcaggcgtc ccggccaggc cggtatcgac gaggaccgtg
     2641 tggtcctccc cggttaggag gtactggcac atgaagcgta cccccaggtc cgactcgatc
     2701 cgatggatgc ccggcagtat ctccactctc
//
LOCUS       DASTUV010000445         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_155104, whole genome shotgun sequence.
ACCESSION   DASTUV010000445 DASTUV010000000
VERSION     DASTUV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155104"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFT03_08980"
     CDS             complement(<1..699)
                     /locus_tag="VFT03_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4848320.1"
                     /translation="MTEQQTSVAPSELEDFLRKYNKGLRIVVEGAQADTGQTPKATVW
                     TKNKAKLYRYEPEKPKKYPVPILIVYALINRPYVLDLIPGNSLIEYLVGEGFDVYMLD
                     WGIPGDEDKDLNFEDYVLDYLPRAAKKVMRISGSEEYTLFGYCMGGTMSAMYASLFPE
                     HLKNLVLLTTPASFPREEIGLYKIFTDSKYLSPGLLADAFGNIPGEMIDTGNRMLKPV
                     TNYVGTYVSMWERIF"
     gene            complement(696..1604)
                     /locus_tag="VFT03_08985"
     CDS             complement(696..1604)
                     /locus_tag="VFT03_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEU4848321.1"
                     /translation="MTEERRLGTSVDPAELWRRWFETGTRAWADMLQGEGYSDPFGLY
                     RKWFEGAEIARRQMTGATAANGTESAGNADPRELWRRWFDASVESWEKSAELGGEMLN
                     VTPRYFEMLEQARTNLMSAGSFPKDPLEFAVHWYNATSGPFSEFIQDVIEREEFLEPA
                     SEWLEHYASFYKLFARRSEEYLKSLQVPVRSDITRVAGLVVALEDKVDRLEEAFEEFE
                     YGYAKPATAEELGELEKRFDRVEGKLDRLLAALENGNAQNGAPEATDAARRKARELGV
                     DLTEIVGTGAGGQITVDDVRRRGGAR"
     gene            complement(1855..2235)
                     /locus_tag="VFT03_08990"
     CDS             complement(1855..2235)
                     /locus_tag="VFT03_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848322.1"
                     /translation="MLKPAEGPTTVAILGENPVVGNALESLLRSTDFAARFFPEYLPN
                     SAEPFEGARVALILPALSSKRNEALIAGIRSHPATVDLPVIELITTPNGGPHEHIRLP
                     WPCGLEELIRSIEAALPHGCGSQL"
     gene            complement(2369..>2849)
                     /locus_tag="VFT03_08995"
     CDS             complement(2369..>2849)
                     /locus_tag="VFT03_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848323.1"
                     /translation="TRELKRELPDIAVLIMTMHENMDYLLEALDAGAAGYVLKDSPAE
                     RLISAVRRTLKGESPLNQELAAQLLRRLAEERKSDPAPKPPEPRESLREPLTPRELEV
                     LGLLATGRTNQQIAQTLVISKGTVKVHVERIIRKLNVSDRTQAAVRAIEMGLLTPES"
BASE COUNT          458 a          908 c          887 g          596 t
ORIGIN      
        1 gaagatgcgc tcccacatgc tgacgtaggt accgacgtag ttggtgaccg gcttgagcat
       61 cctgttgccc gtgtcgatca tctcgccggg gatgttgccg aacgcgtcgg ccagcagccc
      121 cgggctcaag tacttgctgt cagtgaagat cttgtagaga ccgatctcct cgcgcgggaa
      181 agatgccgga gtggttagca gaacgaggtt cttgaggtgc tcggggaaga gggaggcgta
      241 catggcgctc atcgtcccgc ccatgcagta gccgaacagg gtgtactcct cggagcccga
      301 gatcctcatg accttcttgg ccgcccgcgg caggtagtcg agcacgtagt cctcgaagtt
      361 gaggtccttg tcctcgtcgc cggggatgcc ccagtcgagc atgtagacgt cgaagccctc
      421 gcccacgagg tactcgatca ggctgttgcc cgggatgagg tcgaggacgt aggggcggtt
      481 tatgagggcg tagacgatga ggatcgggac cgggtacttt tttggcttct cgggctcgta
      541 gcggtagagc ttggccttgt tcttggtcca gacggtcgcc ttgggagtct ggcccgtgtc
      601 ggcctgggca ccctcgacga cgatcctcag tcccttgttg tacttcctga ggaagtcctc
      661 gagctccgag ggcgcgacgc tcgtctgctg ctccgtcacc ttgcccctcc tctcctgcgc
      721 acgtcgtcaa ccgtgatctg gccccctgct ccggtaccca cgatctccgt gaggtccacg
      781 ccgagctccc gagccttgcg cctggccgcg tctgtggcct ccggggcgcc gttctgcgcg
      841 ttcccgttct ccagggcagc gaggagccgg tccagcttcc cctccaccct gtcgaagcgc
      901 ttctccagct cgccgagctc ctccgcggtg gcgggcttgg cgtagccgta ctcgaactcc
      961 tcgaaggcct cctccagacg gtctaccttg tcctcgagcg ccacgacgag gcctgcgact
     1021 cgcgttatgt cagaacggac cggtacctgg aggctcttca aatactcctc ggaacggcgc
     1081 gcgaaaagct tgtagaagct cgcgtagtgc tccagccact cgctggcggg ctcgaggaac
     1141 tcctcccgct cgataacgtc ctggatgaac tcggagaacg ggccgctggt cgcgttgtac
     1201 caatgcacgg cgaactccag cggatccttc gggaagctgc cggcgctcat caggttggtc
     1261 cgcgcctgct ccagcatctc gaagtagcgc ggggtcacgt tcagcatctc cccgccgagc
     1321 tccgcggact tctcccagga ctctacactc gcgtcgaacc acctgcgcca cagctcccgc
     1381 ggatcggcat tccccgcgct ctctgtgccg ttggccgccg tggcgcccgt catctgcctg
     1441 cgcgcgatct ccgctccctc aaaccacttc ctatacaggc caaaggggtc cgaatagccc
     1501 tctccctgca gcatgtcggc ccacgccctg gtgccggtct cgaaccaccg tctccagagc
     1561 tccgccgggt ccacgctggt cccgagccgc ctctcttccg tcaagcccat acctcctggt
     1621 ctctcacgtc aaacgcgcct cgcagcgccc ggacggcaac ccgcccggat gctctccgcg
     1681 gcccctctgc gccagattat acttgaaaaa cggaggtata tgttatccac acataccgca
     1741 agcgtagccg ggctaacagg cctgtcgcga cgccttgcat ggcctggaat ctctattggt
     1801 cgattgggat gcgaaggtgg cagtggggct tgacagtggc aggtggtatg cgatctagag
     1861 ttgcgaaccg cagccgtgcg gcagagcggc ttcgatgctt cgtatcagct cctccagacc
     1921 gcagggccac ggtaggcgga tgtgttcgtg cgggccaccg ttgggggtcg tgatcagttc
     1981 gatgaccggc agatccaccg tcgctgggtg actcctgata cccgctatga gggcttcgtt
     2041 gcgcttggag ctcaaggcgg gcaggatgag cgcaacccgg gccccttcga acggctcggc
     2101 ggaattgggc aggtattctg ggaagaatcg cgcggcgaaa tcggtggaac ggagcagtga
     2161 ctccagagcg ttgccgacca ccggattctc gccgaggata gccacggtag tcggtccctc
     2221 ggccggtttc agcatcttcg ggcgcctctc agccgtctta ccaggctcat gcactaagta
     2281 tggggtgaca aggggcattt gcgtatacgc cgggtggcgt attttgcgta tgccacctgg
     2341 catattcgtc ctggatggtg gcttcttatc aggattcggg agtcagcaga cccatctcga
     2401 tagcgcgaac ggccgcctgg gtgcggtcgg agacgttcag cttcctgatt atccgctcga
     2461 cgtgaacctt gacggtcccc ttgctgatta ccagtgtctg ggcgatctgc tgattggtcc
     2521 gcccggtcgc aagaagtccc aggacctcga gttcgcgcgg tgtaagcggt tcgcgcaacg
     2581 attcgcgtgg ctccggtggt ttcggggccg gatcgctctt tctttcttcg gccaggcgcc
     2641 ggagaagctg ggctgcgagc tcctgattca agggtgactc gcccttcaag gtcctgcgaa
     2701 cggcgctgat caggcgttcg gcagggctgt cctttaggac atagccggcg gcgcccgcgt
     2761 ccagagcctc taagaggtag tccatattct catgcatggt cattatgagc acggcgatgt
     2821 cgggaagctc gcgcttgagc tcgcgggtc
//
LOCUS       DASTUV010000446         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_334550, whole genome shotgun sequence.
ACCESSION   DASTUV010000446 DASTUV010000000
VERSION     DASTUV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334550"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1276
                     /locus_tag="VFT03_09000"
     CDS             287..1276
                     /locus_tag="VFT03_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU4848324.1"
                     /translation="MLRFLLVAVLVLCALPGCGPGKGVTGDKPETTGRPAQEAEGTAP
                     QNVAEAAAASVDESSIRTHLARLTGASPAPLDGREVTISERGTESGRRAAAGYMKSSF
                     EEMGIPARIHEFRSGGLRGFNVEATLRGTGDGRHLWVTAHLDSVHNAGANDDASGLTS
                     ILLTAKALEEIGPEHTVHFVAYDLEEVGLFGSTHYMENTVKGIVEREGERAIIGALQS
                     DMIAYEEDEFDAVLGTCGRAGPIDDALSRAAEEIDSPLDLRELCLGRSDHESFWDAGL
                     PAAVLTDGAIYDGYPCYHMPCDTVDELNISYLRSMIQLTAAAAALLAAPQGEG"
     gene            complement(1317..1390)
                     /locus_tag="VFT03_09005"
     tRNA            complement(1317..1390)
                     /locus_tag="VFT03_09005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Met,seq:cat)
     gene            1474..1854
                     /locus_tag="VFT03_09010"
     CDS             1474..1854
                     /locus_tag="VFT03_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848325.1"
                     /translation="MFLISILAGAVLALFAAIGALRAYFRLRRARAALQSTLTEEVDR
                     LSRRTGELEKNLSTLDARAQQLPIEIAELQKSLSTLRVLTAALGATLQQVQRVLSYSA
                     LKTLSAGRIADLLRYRPKNDPGSG"
     gene            2080..2685
                     /locus_tag="VFT03_09015"
     CDS             2080..2685
                     /locus_tag="VFT03_09015"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU4848326.1"
                     /translation="MWENIACRLEGSLVVLEPLKRSHEQGLFEAARDPRIWRYIPFDA
                     GGTSDLSESPTAFRSWLDVALAASEAGDEAAFATLDAESGEPIGSTRYLALRPAHRGL
                     EIGWTWLTPSRWRTGANVEAKLLMLEHAFGSLGCVRVEFKTDARNERSRAALEALPAQ
                     FEGVFRKHMILPGDRYRDSAYYSIVDDEWPDVRANLRRRLA"
     gene            complement(2707..3159)
                     /locus_tag="VFT03_09020"
     CDS             complement(2707..3159)
                     /locus_tag="VFT03_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4848327.1"
                     /translation="MGSRRTDRHFTVAVFVVWEGKVLLHWHRKLEMWLPPGGHIEDGE
                     LPDEAALREVREETGLWAELVGERREDIADPAQLHRPAGVQLENIGPGHQHIDLIYFA
                     KPSGSTEIQEAFSGAKVGWYGPEDWEGMLINAEVRGWCERALATVGAW"
     gene            complement(3253..>3522)
                     /locus_tag="VFT03_09025"
     CDS             complement(3253..>3522)
                     /locus_tag="VFT03_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEU4848328.1"
                     /translation="EFLEVSVEETRWEKILEYCSFDWMKRHATKSVPLGGAFWDAGAQ
                     VFINKGVNGRWAEILTLEDVAEYEARAVQELGPECALWLATGEEL"
BASE COUNT          618 a         1195 c         1116 g          593 t
ORIGIN      
        1 cgtgaagacg acggtgacct cttccccggt ctccttgaac tcgtaggctg tggtgagggc
       61 gttggctact ttcgccccat ccgcgcgcgt ctcggtgtcc gccaggatta cgatggctac
      121 cttggccgcc acactattcc cctccccggt tcgtctcacg cctagtttcc ccgagccggc
      181 cctcgccaaa ccgcatcatc gccgtgcgag gcgttcggtt atgatcccgg cgaggccgaa
      241 ccgtagcggc aggagcgaag atggcgggtg ggtaatgacg aagatcatgc tccggttcct
      301 cctcgtcgcg gttctggtcc tctgtgcgct cccaggctgc ggacccggca aaggagtaac
      361 tggagataag cccgagacga ccggacgacc ggcccaagag gccgagggca cagcgcccca
      421 gaacgtggct gaggctgctg cggcgagcgt cgacgagtcc tcgatccgta cccacctggc
      481 ccgtctgaca ggcgcatccc cggctccgct cgatggtcgc gaggtcacga tctccgagcg
      541 tggaaccgag agcgggagga gagctgccgc cgggtacatg aagtcctcct tcgaggagat
      601 gggaattccc gcgcgcatcc acgagttcag atctggtggc ctgcgcgggt tcaacgtgga
      661 ggcgaccctg cgcgggacag gagacgggag acacctgtgg gtgacggctc atctcgactc
      721 cgtacacaac gcaggggcca acgacgacgc cagcggcctg acctcgatcc tcttgacggc
      781 gaaggcgctc gaggagatcg gaccggagca tactgtccac ttcgtggcct acgacctcga
      841 ggaggtcggc ctctttggga gcacccacta catggagaac accgtcaaag gcatcgtgga
      901 gcgggaaggg gagcgtgcca tcatcggggc cctccagagc gacatgatcg cctacgaaga
      961 agacgagttc gacgcggtgc tcgggacctg cggcagggcc ggtcccatcg acgacgctct
     1021 ctcccgggca gcggaggaga tcgactcccc tctagacctc agagagttgt gcctgggacg
     1081 ctctgaccac gagtctttct gggacgcggg gcttccggca gccgtgctca ccgacggcgc
     1141 aatctacgat ggctaccctt gctaccacat gccctgcgac accgtagacg agctcaacat
     1201 ctcgtacctg cggtcgatga tccagcttac cgccgcagcg gccgcgctcc tcgcggcccc
     1261 gcagggcgaa ggttgagggc acgaaaaagg ccccgcgaag ccgggacctg ggttagtagc
     1321 gggggcagga tttgaacctg cgaccttcgg gttatgagcc cgacgagcta ccagactgct
     1381 ccaccccgcg tcgctgtgaa aggcagttta ccaccggtta acagattgta aagcgacgcc
     1441 ggagcgtggt acggtggcct cgagatgggc accttgttct tgatctcgat cctcgccggc
     1501 gcggtgctcg ccctctttgc cgccatcggc gctctgcggg cctacttccg gctccgccgc
     1561 gctcgcgccg ccctccagag caccctcacc gaggaggtgg atcgcctctc ccggcgaacc
     1621 ggcgagctgg agaagaacct ctccaccctc gacgcccgcg cgcaacagct ccccatcgag
     1681 atagccgagc tacagaagag cctctcgacc ttgcgggtcc tcaccgcggc gctgggggcc
     1741 acgctccaac aggttcagcg ggtcctctcc tacagcgcgc taaagacttt gagcgccggc
     1801 cgcatcgccg acctgctccg gtaccgcccg aagaacgatc caggctcggg ctgagggtgt
     1861 cggacgcgcc gcgcgcccgt atactcctcc agatgggcga agaacagacg cagcaacacg
     1921 aaaccgggga ggcgcctgag ccttccggcg aggactgcga agtgtgcggc tcggagatgt
     1981 acgggttgca ctgcaagctc gtgtgcccca actgcggtta tcgccgcgac tgctccgacc
     2041 cctgatgccc tgagagacca tcagcgcagg gagggacgca tgtgggagaa catcgcgtgc
     2101 cggctggagg gctcgctagt cgtcctcgag ccgctgaagc ggagccacga gcagggcctc
     2161 ttcgaggctg ctcgggatcc gaggatctgg cgctacatac cgttcgacgc gggcgggacc
     2221 agtgacctgt cggagagccc aaccgccttc cgttcctggc tcgacgtggc gctcgcggca
     2281 tcggaagcgg gagatgaagc ggcgttcgcg acgctcgatg ccgagagcgg cgagcccatc
     2341 gggagcaccc gctacctggc gctccggccg gcgcaccgcg ggctggagat tggctggacc
     2401 tggctcaccc cttcccgctg gaggaccggc gcgaatgtcg aggcaaagct gctcatgctc
     2461 gaacacgcct tcggttcatt gggctgtgta cgcgtcgagt tcaagaccga cgcgcgcaac
     2521 gagcgatcca gagccgctct cgaggccctc cccgcacaat tcgagggcgt attccgcaaa
     2581 cacatgatcc tgcccggaga ccggtaccgc gactctgcct attacagcat cgtcgacgac
     2641 gagtggcctg acgtcagagc gaacttgagg cggcggctcg cgtagggggc tgttcgcgaa
     2701 ccgcccctac cacgcgccga ccgtcgcgag cgcccgctca caccagcccc tgacctcggc
     2761 gttgatgagc atgccctccc agtcttcggg accgtaccag ccgaccttcg ctccggagaa
     2821 ggcttcctgg atctccgttg agcccgaagg ctttgcgaag tagatcaggt ctatgtgctg
     2881 gtgtccgggc ccgatgtttt cgagctgcac cccggccggt cggtgcagct gcgcggggtc
     2941 ggcgatgtcc tcgcgccttt ccccaacgag ctcagcccac agccccgtct cctccctgac
     3001 ctcgcgcagc gcggcctcat cgggcagctc gccatcctct atgtgcccgc ccggcgggag
     3061 ccacatctcc agcttgcggt gccagtgcag gaggaccttc ccctcccaca cgacgaagac
     3121 agcgaccgtg aagtgcctgt cggtacgccg ggatcccata agttagaagg gtacaacagg
     3181 agatccgggc cgcacctgtc ctctgtccgc gagaggaaac gaatcgggga ccggtggttc
     3241 ttcggccggg acctagagct cctctcccgt tgcgagccat agcgcgcact cggggccaag
     3301 ctcctgaacg gctcgcgcct cgtactccgc tacatcctcc agcgtgagga tctcagccca
     3361 acggccgttc acccctttgt tgatgaatac ctgggcgccg gcgtcccaga aggcgccccc
     3421 gaggggaacg ctcttggtgg cgtgccgctt catccagtca aaggagcagt actcgaggat
     3481 cttctcccac cgcgtttcct cgacggacac ttcgaggaac tc
//
LOCUS       DASTUV010000447         4108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_977948, whole genome shotgun sequence.
ACCESSION   DASTUV010000447 DASTUV010000000
VERSION     DASTUV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977948"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFT03_09030"
     CDS             complement(<1..929)
                     /locus_tag="VFT03_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU4848329.1"
                     /translation="MIDFGWTRLAYLLVVLSVLAALARLGGAGADPRPLAAEPPDKAP
                     VAEVPPADVMGSHEHSTPQAGAAPRENPASPAFARHLRRATVGGASAASEAGRAVFYR
                     PSPPGPQSGAAAQGPGPSCGNLEGFPSGDRLVFPLPMGYANSYDDTWGAPRPQGGHEG
                     TDLMAPAGTPEYAVTDGTIVPVTGSNGNGWNTLGGYALMLRAAYSVGPVREGDLFYYA
                     HLEKESALPLGASVRAGQVVGYAGDTGQGPEVTRGLFPPHLHLGWYDATGARSYLPSG
                     AMNPYPLLEWITAGGGVLGGGSSARYCEVPRSGP"
     gene            complement(1020..2009)
                     /gene="tsaD"
                     /locus_tag="VFT03_09035"
     CDS             complement(1020..2009)
                     /gene="tsaD"
                     /locus_tag="VFT03_09035"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU4848330.1"
                     /translation="MILAIETSCDDTCAAVVETDGRRALSNVVHTQTEHDRYGGVVPE
                     VASRAHLERIDGVIQSSLADAETSLEDVESVAVTVRPGLIGALLVGLSAAKGIAYARS
                     LPLVPVNHLEGHVAAAYLADPALEPPFVALIASGGHTALYSVDRNHHMQLLGETLDDA
                     AGEALDKGARMLGLGFPGGPALQKAAQGGDRNRYDFPVALRGKDNLNFSFSGLKTSLL
                     YKLKDLGEERTREELPHLAASYEAAVVEALVRKLMRASDIEDAQVVVVAGGVAANARL
                     RRTLQEESERLGAKLIIPPPELCTDNAAMIGAAAPHSSAIAYPHYLSLNARSV"
     gene            2099..2347
                     /locus_tag="VFT03_09040"
     CDS             2099..2347
                     /locus_tag="VFT03_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848331.1"
                     /translation="MRTFSLLFTFIALVILAIFLIYVVRGPVDPLIAGGIFVSFLACL
                     VPALLLLKPFEPPEALKHRREEREKRRSSSDEPGDSGS"
     gene            complement(2316..2765)
                     /gene="rimI"
                     /locus_tag="VFT03_09045"
     CDS             complement(2316..2765)
                     /gene="rimI"
                     /locus_tag="VFT03_09045"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU4848332.1"
                     /translation="MSETRPHLRPMEASDLASAIEVDTLCLPRPWSEAIWREEIESPF
                     TLFLLLEEEGEILAQIGVRYLADDLHVTTLAVRPDHRRRGYAHVLLEAALGAFPEARC
                     VHLEVRPSNVPARSLYESLGFVTTGRRRRYYGDEDALLMTRYLPARR"
     gene            complement(2762..3436)
                     /gene="tsaB"
                     /locus_tag="VFT03_09050"
     CDS             complement(2762..3436)
                     /gene="tsaB"
                     /locus_tag="VFT03_09050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU4848333.1"
                     /translation="MLILAFDASTPVITVAVARAGGEAREVLAEVSVSARGASETLLP
                     AIDAALDLAGERLGSVELVLAGVGPGTFTGIRIAATTARALALATGAALSKNSTLAAL
                     ASPSLLGGHPDVLAVLDARRGQVFAQRFPAGGPASHIYCERPEDLPVEGAPLVVGDGA
                     VRYRAALSALGSIPPDDSPLHRVTATGHVLSADLAPVEADDLVPIYVREPDAEVRRDL
                     NPWSRP"
     gene            complement(3430..3888)
                     /gene="tsaE"
                     /locus_tag="VFT03_09055"
     CDS             complement(3430..3888)
                     /gene="tsaE"
                     /locus_tag="VFT03_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU4848334.1"
                     /translation="MSVELEGLDETALERLAASLAARLEPGDVVVLAGEVGSGKTTFV
                     RAAARTLGVEGRVTSPTYQIARGYEGAVEGRPVTVNHLDLYRLEGLEDRDALELDDYL
                     DPGAVTFVEWASREALGLIENPTVVRIAHQTPSTRRVSVSGPAAERPSRC"
BASE COUNT          658 a         1329 c         1455 g          666 t
ORIGIN      
        1 gggccggagc gcgggacttc gcagtagcgg gcgcttgatc caccgcccag gacgccgccg
       61 ccggccgtga tccactcgag caaggggtag gggttcatgg cccccgaggg gaggtagctc
      121 ctcgcacccg tcgcgtcgta ccacccgagg tgcaggtgcg gcgggaagag accgcgtgtc
      181 acctcaggcc cctgacccgt gtcgccggcg tacccgacca cctgcccggc ccggactgat
      241 gcgccgagcg gcaaggcgct ctccttctcc aggtgcgcgt agtagaagag gtcgccctcc
      301 ctgaccggtc ccacagagta ggcggccctc agcatgaggg cgtagccgcc cagagtgttc
      361 cagccgtttc cgttcgagcc ggttacaggc actatggtgc cgtccgttac ggcgtactct
      421 ggcgtgccgg ccggagccat cagatccgtc ccctcgtgac cgccctgtgg ccggggcgcc
      481 ccccaggtgt catcgtagga gttcgcgtac cccatgggga gcgggaagac gagcctgtcg
      541 ccgctcggaa atccctcgag gttgccgcag gacggtcctg gaccctgcgc ggcggcaccg
      601 gactgcgggc ccgggggaga cggacggtag aagacggccc ggccggcctc ggaagccgcg
      661 gacgcacccc cgacggtagc ccttctcaga tgcctggcga aagcaggtga cgcagggttc
      721 tcgcgtgggg ctgcgcccgc ctgtggtgtg gagtgctcgt gcgagcccat tacgtccgcg
      781 ggagggacct ccgcaaccgg tgccttatcc gggggttcgg cggccagggg ccgggggtcg
      841 gcacccgcgc ctcccagccg ggcgagggcc gccagaacgg aaaggacgac cagcaggtaa
      901 gcgagcctcg tccatccgaa atctatcacg cgttctcccc cgtaaccctg ttcagcgctg
      961 gactgtactc tcacagatgg ggcatcgggc gtcaaggcga gacgatgccg ggcgggtctt
     1021 cagacgctgc gggcgttcag gctcaggtag tgggggtagg cgatggcgga cgagtgtgga
     1081 gcggcggccc cgatcatggc tgcgttgtcc gtgcacagct cgggcggcgg gatgatgagc
     1141 ttcgcaccaa gcctctcaga ctcctcctgc agggtccgtc gcagccgtgc gttggcggcg
     1201 acaccccccg cgacgactac cacctgcgca tcctcgatat ccgaggcgcg catgagcttg
     1261 cgcacgagtg cttcgacgac ggccgcctcg tagctcgccg ccaggtgcgg caactcctcg
     1321 cgggtccgct cctcgccaag atccttcagc ttgtagagca gactcgtttt caggcccgag
     1381 aagctgaagt ttaggttgtc cttccctctc aaggccacgg ggaaatcgta gcggttcctg
     1441 tcgccgccct gggctgcctt ctgtagcgcg ggtccgccgg ggaagccgag cccgagcatg
     1501 cgggcgccct tgtcgagggc ctcccccgct gcgtcgtcca gggtctcccc gaggagttgc
     1561 atatgatggt ttcggtccac ggagtagagg gcggtgtgac cgccggaggc gatcagggcg
     1621 acgaaaggcg gctcgagtgc tgggtcggcc aggtaagcgg cggcgacgtg accctcgagg
     1681 tggttcaccg gcacgagcgg gagcgagcgc gcgtaggcaa tgcctttcgc cgccgagagg
     1741 ccgacgagga gcgcgccgat caggcccggc ctcacggtca cggcgacgct ttcgacgtct
     1801 tcgagagagg tctcggcgtc ggcgagggaa ctctggatca cgccgtcaat ccgttcgagg
     1861 tgggcgcggg aggcgacctc ggggacgacg ccaccgtagc ggtcgtgctc tgtctgagta
     1921 tggacgacgt tggagagagc gcgccggccg tctgtctcga cgacggcggc gcaggtgtcg
     1981 tcgcacgaag tttcgatggc aaggatcatg gactttttat aacacccagg acgactgatg
     2041 ggttctcaga tggagtgcgc actacactga cggcccgcgc gggtatactc gggtcgccat
     2101 gcggaccttc tcgctgctgt tcaccttcat agctctggtg atcctggcga tattcctgat
     2161 ctacgtggtc aggggtccgg tcgatcccct gatcgccggc ggcatcttcg tttcttttct
     2221 tgcctgtctg gtacctgcgc ttcttctcct taagcccttc gagccgcccg aggccctcaa
     2281 acaccgcagg gaagagcgcg agaagcgccg gagctctagc gacgagccgg gagatagcgg
     2341 gtcatgagca gcgcgtcctc atcgccgtag tagcggcgac ggcggccggt cgtgacgaag
     2401 cctagagact cgtagaggga gcgggcgggg acgttgctgg ggcgtacctc caggtgtacg
     2461 cagcgggctt cggggaaagc accgagcgct gcttcgagga ggacgtgggc gtatcccctg
     2521 cggcggtggt cggggcggac ggcgagcgtc gtgacgtgca ggtcgtcggc caggtacctc
     2581 acccctatct gggcgaggat ctcaccctct tcctcgagga ggaggaagag ggtgaatggg
     2641 ctctcgatct cctcgcgcca gatcgcctcg ctccagggtc ggggaaggca cagagtatcc
     2701 acctctatgg cggaggccag atccgaagcc tccatcgggc gcaagtgtgg ccgcgtctcg
     2761 ctcacgggcg gctccagggg ttgaggtcac gcctgacctc ggcgtccggc tcgcggacgt
     2821 agatggggac gagatcatcc gcctcgacgg gcgcgaggtc ggctgagagt acgtgcccgg
     2881 tcgcagtcac tcggtgcaac ggcgagtcgt ccggcgggat gctacccagg gccgagagcg
     2941 cggccctgta gcgcaccgcg ccgtcgccga cgacgagcgg agcaccttcc acagggaggt
     3001 cctccggcct ctcacagtag atatggctcg ccggtccgcc cgccgggaac cgctgggcga
     3061 atacctggcc ccgcctggcg tcgaggacgg cgagcacgtc gggatggccg cccaagagag
     3121 aaggggatgc gagggccgcc agggtggagt tcttcgagag ggcggccccc gtcgccagag
     3181 cgagcgccct ggccgtggtg gcggcgatgc ggatgccggt aaaggttccg ggccccacgc
     3241 cggccagcac gagttccacc gagccgagtc tctcacccgc gagatccagc gcggcgtcga
     3301 tggcggggag cagggtttcg gaggcgccgc gggcggagac cgagacctcg gcgaggacct
     3361 cgcgcgcttc ccctccggct ctggcgacgg cgacggtgat caccggagtc gaagcgtcga
     3421 aggcgaggat cagcacctgc ttggacgctc cgcggcgggg ccggatacgc tcacccggcg
     3481 cgtcgacggc gtctggtggg cgatacggac gacggtcggg ttctcgatga ggccgagtgc
     3541 ctccctcgag gcccactcga cgaaggtgac ggcgccgggg tccaagtagt cgtcgagctc
     3601 gagcgcgtcc ctgtcttcga ggccttcgag gcggtagagg tcaagatggt tcaccgtcac
     3661 cgggcgaccc tccacggcgc cctcgtagcc acgcgcgatc tggtaggtcg ggctcgtcac
     3721 ccgcccttcg acgccaagag tgcgggccgc ggcccgcacg aaggtcgtct tgcccgagcc
     3781 tacctcaccg gcgagcacga cgacgtcccc aggctcgagg cgggcggcca ggctcgcagc
     3841 cagccgttcg agcgcggtct cgtcaagacc ttcgagttcc acactcacta atcaggcctc
     3901 catgagaacc gaggggacgt accagttaaa gggccgaagc atcgctgccg atacaataca
     3961 accagcgcaa tgttcacgac tttcgagtac agacgactcg cgccatctgt acaacgaaat
     4021 gctcgagcaa attgagcgtg tcccgagaag ccggttgctc tagcttccgg agtcgtcacc
     4081 agaggccggg ttggaggggt tcgcgctc
//
LOCUS       DASTUV010000448         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1337465, whole genome shotgun sequence.
ACCESSION   DASTUV010000448 DASTUV010000000
VERSION     DASTUV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337465"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFT03_09060"
     CDS             <1..1161
                     /locus_tag="VFT03_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU4848335.1"
                     /translation="PLSAKGLADAKALGESIERTGEIANVESVYTVGEEAATSYAQRV
                     AEARKDARAEADRRVEEAVKGELQSLRTRYGRVPPGAEGRIRAEAERRASAEVDRKVP
                     ELPEGVSTEGEISPQGVANFLDSPRARESKELQDALDSYVAGDRTLLQAVTKANPYTE
                     AAHAAVDEVRSVEAPEGVSFLVGGLSAGQKDFISSLYGKAPYAAAFVLSVTYLILLVS
                     FRSVFIPLKAVIVNILSLTASFGAIVFVFQDGNLSGLLDFTPLGFVDATLPILMFCTI
                     FGVSMDYEVFLLSRIREAYENGDSNTASVAKGLVATAGIITSAAAIIIVVTGAFAFTG
                     IILTKAVGLGLAVAVFVDATIIRVLLVPATMRILGDWNWWPGGRKKTFGADS"
     gene            1266..1679
                     /locus_tag="VFT03_09065"
     CDS             1266..1679
                     /locus_tag="VFT03_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU4848336.1"
                     /translation="MVEFERILRDRGYRLTNQRRVIVRGLEGDRHLSAEEIYDRVKGE
                     HPEIGLSTVYRTLDLLHELGIVRKEDFGEGYSRYELATERVHHHARCRECGAVIEFNE
                     ELMEYLALQVERETGFVSDWYEITLHGRCASCGDS"
     gene            complement(1817..>2009)
                     /locus_tag="VFT03_09070"
     CDS             complement(1817..>2009)
                     /locus_tag="VFT03_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848337.1"
                     /translation="HMTPAAVARMASESGVRQIVLTHIYPAADALDLPAEVGRGYNGE
                     VVVAQDGSKLSVPNNSRNN"
BASE COUNT          347 a          616 c          668 g          378 t
ORIGIN      
        1 ccgctgagcg ccaaggggct cgctgatgcg aaggctctgg gcgaaagcat cgagcgtaca
       61 ggcgagatcg cgaacgtcga gagcgtctac acggtggggg aggaggcggc gacgagctac
      121 gcccaacgcg tcgccgaagc ccgcaaggac gctcgggccg aggccgaccg ccgggtcgaa
      181 gaagccgtga aaggtgagct gcagagcttg cgaacgcggt acgggagggt cccgccgggt
      241 gctgagggca ggatcagggc cgaggccgag cggcgcgcct ccgcggaggt cgacagaaag
      301 gtccccgagc tgcccgaggg agtctcgacg gaaggcgaga tctcaccaca aggcgtcgcc
      361 aacttcctcg actctccccg ggcgcgcgag tcgaaagagt tgcaggacgc gctggactcg
      421 tatgtggcgg gagataggac cctgctccag gcggtcacga aggccaaccc gtataccgag
      481 gccgcccacg ccgccgtgga cgaggtgcgt tccgtggagg cgcccgaggg cgtgagcttc
      541 ctggtcggtg gcctttccgc cgggcagaag gattttatct ccagcctcta cggcaaggcc
      601 ccttacgcgg cggcgttcgt tctgagcgtt acctatctca tactcctcgt tagcttccgc
      661 tccgtgttca tcccactcaa agcggtcata gtgaacatcc tgagcctgac ggcgagcttc
      721 ggggcgatcg ttttcgtctt tcaggacggc aacctctccg gtttgctcga cttcacgccc
      781 ctgggcttcg tggatgccac actgccgatc ctgatgttct gcacgatctt cggagtctcg
      841 atggactacg aggtcttcct gctctcccgc atccgcgaag cctacgagaa cggggactcc
      901 aacaccgcga gcgtcgccaa ggggcttgtc gccaccgccg gtatcatcac gtccgcggcg
      961 gcgatcataa tcgtcgttac cggagccttc gcttttaccg gcatcatcct taccaaagcc
     1021 gtcggcctcg gactggccgt cgccgtcttc gtggacgcga cgatcatccg ggtcctcctc
     1081 gtccctgcaa ccatgcggat cctgggcgac tggaactggt ggcccggcgg acgcaagaaa
     1141 acgttcggcg ccgattcgta gagacccgtc gggtagttga ccggcgccga ctcgagcctt
     1201 ggagccttca gctcgggata agatcaattc ttgatactgg tcggagctag ggctacacta
     1261 cgtacgtggt cgagttcgag cggatactca gggaccgtgg ctaccggctg accaaccagc
     1321 ggcgggtgat cgtgcgtggg ctggagggcg accggcacct ctccgcggag gagatctacg
     1381 acagggtcaa gggagagcac ccggagatcg gactctcgac ggtctacagg acgctggacc
     1441 ttttgcacga gcttggcatc gtgcgcaagg aagacttcgg cgaggggtac tcgcgctacg
     1501 agctcgcgac ggagcgggtg caccatcacg cccgttgccg ggagtgcggg gcagtcatag
     1561 agttcaacga ggagctcatg gagtacctgg cgttgcaggt cgagcgtgag actggcttcg
     1621 tctcggactg gtatgagatc acgcttcacg gccgctgcgc gagctgcggc gattcttagc
     1681 acgctcaggc tacgccgtcg cttgtcagca cgcctccctt cggtcggctt gtcagcacgt
     1741 cgcccggggt tcgcctcagg cgacttgtca gcttgttgtt ttctgagggg cggggtattc
     1801 gaggttcttg cagggatcaa ttatttcgag aattgttggg gacgctgagc ttcgacccgt
     1861 cctgggccac tacgacttcg ccattgtacc cgcgccccac ctcggcaggg aggtccagcg
     1921 cgtccgccgc cgggtagatg tgtgtgagta cgatctgcct gacgccggat tccgaggcca
     1981 tcctcgctac ggcggccggc gtcatgtga
//
LOCUS       DASTUV010000449        16016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_12461, whole genome shotgun sequence.
ACCESSION   DASTUV010000449 DASTUV010000000
VERSION     DASTUV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16016
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12461"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VFT03_09075"
     CDS             <1..138
                     /locus_tag="VFT03_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU4848338.1"
                     /translation="AAFLYPWAVIYRELGLYGFVEMVIFVLILGVGLAYAWKKGALKW
                     V"
     gene            138..959
                     /gene="nuoB"
                     /locus_tag="VFT03_09080"
     CDS             138..959
                     /gene="nuoB"
                     /locus_tag="VFT03_09080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEU4848339.1"
                     /translation="MQQFNEPNLITTSSDKLFNWARKNSLWPLQFGLACCAIEMMSTG
                     MAHNDLDRFGAGFFSASPRQADVMIVSGTVSTKMAERMTRLYEQMPEPKWVIAMGACA
                     ISGGPFLDGYSVLMGADRVIPVDVYVPGCPPRPEALIFGIMTLQKKIERDQQVGYEKP
                     RPALVNEDGSINEYLPEREYQHIAEGKAPRFEGIRPLKRTYVFERKGMPPASQTDVGW
                     IPAEAKKKLKEKEKAEKAAQENGGAGGKAEASAEANGKSGGEAKAGTDGAKKEDA"
     gene            952..1452
                     /locus_tag="VFT03_09085"
     CDS             952..1452
                     /locus_tag="VFT03_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU4848340.1"
                     /translation="MPEVAVTLPEGDLETRLERYLEGLRNRHGLEESGIEGNVVRVLV
                     DTESLPAVLATAREALGILHLSFITVVDREGSFELTYELLDFRGARVRVKTVVEGTEP
                     VVPTVTNVYPTANWHEREAYDMFGIAFRGHPDLRRTYMWQDHFDHHPLLKSLEISTKR
                     TFEGLR"
     gene            1456..2640
                     /locus_tag="VFT03_09090"
     CDS             1456..2640
                     /locus_tag="VFT03_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU4848341.1"
                     /translation="MLGEERRTDHSALTEPASGRLVNPDVRDEFGLETIDMSMGPQHP
                     AMHGLLRLILELDGETVVRCDPVMGYLHRCQEKIAENRMYPAVIPLTDRLDYFANMHN
                     EHGYVLAVEDLLDVEIPPRAEYIRVLMCELMRLASHIPSIGFLLLELGAFTPILYAFR
                     ERERIQSIFEAVTGARMMFHYIRIGGVKADLPPEIPEKIWEFVEGLEKNFQSDFVDLI
                     EGNEIFISRTRNVGKMTQEQAVEMGLTGPPLRCTGVDFDIRLDYPYSVYPELKFDVVT
                     DTGGDVEASYRVRIGECLESVKIIKQVLEKMPEGAVMADMGRRIRPPEGEGFGRVESP
                     RGEFAVHVVSDGSDTPYRVHYRDPSFVNMQLLQEIVPGHYLPDIMPIMGLVDPVSGGW
                     DK"
     gene            2628..3641
                     /gene="nuoH"
                     /locus_tag="VFT03_09095"
     CDS             2628..3641
                     /gene="nuoH"
                     /locus_tag="VFT03_09095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU4848342.1"
                     /translation="MGQVVGTVLQYLNQEPLRFLISFGLILFLVLNLAAVLTMAERKV
                     SAYIQLRYGPNRVGPRGLLQPAADVFKLFTKENVSPGRSDLWVFLGAPIAMFLPAALV
                     WLVIPFAPDAVMANLNVGILFFIAVTSIGALGVVMAGYGSRSTFSLLGALRGAAQMIS
                     YEVPLILSLLGVVMLAGSLSFVDVVLAQGDTFWTWYFVPLFPMFIVFYVSGIAEVKRV
                     PFDLPEGESEIVGGFMIEYSGMTWALIQAAEFASMGLMSAITATLFLGGWQPPLPFLD
                     LGAFNWVWFGLKTALIMFTFQWIRWSVPRLRMDQLMDFGWKVLVPITLIYLFIVAAAM
                     LII"
     gene            3655..4167
                     /locus_tag="VFT03_09100"
     CDS             3655..4167
                     /locus_tag="VFT03_09100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEU4848343.1"
                     /translation="MPQVGQGILKGMGVTLKHLFGQKITRQYPEYKRPLPERSRGMLT
                     VDMDRCIACLQCMRICPDHCISIEQEKRDADGSGKAKPYAVGFVIDDSRCMYCSLCVE
                     VCPVNCIYHTEEFEAQAYNRLELARQFGERPVDPTIDPKPTVKKKKKPTGEKAPKTVE
                     RPRTGKAEAV"
     gene            4164..4700
                     /locus_tag="VFT03_09105"
     CDS             4164..4700
                     /locus_tag="VFT03_09105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU4848344.1"
                     /translation="MTVAFAFLAGMTLVSALGVVLSRNVVHSALFMAGSFLGVAGYFV
                     MLNAPALAAFQVLIYVGAVTVVILFGIMFTQKPQVRRYRTIINRQSWWGFFVAAGVGT
                     FLAYVLANEDWGSTSPGHGPRDVGAFGRDLLGVSAGSEVFVLLFEVASVLLLVAIVAA
                     IVISRRKTGEGIDRGVRE"
     gene            4700..5050
                     /gene="nuoK"
                     /locus_tag="VFT03_09110"
     CDS             4700..5050
                     /gene="nuoK"
                     /locus_tag="VFT03_09110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU4848345.1"
                     /translation="MPPEAQQILDALVAAPPLEGYLIVGALLFAIGAWGALVRRNAVV
                     VFMCVELMINGVNLTLVAFGDYLPNAGGLGVSYAVLVIAIAAAEIAVGLAIVLTVFRT
                     RRTVNLDEVTSMRG"
     gene            5062..7047
                     /gene="nuoL"
                     /locus_tag="VFT03_09115"
     CDS             5062..7047
                     /gene="nuoL"
                     /locus_tag="VFT03_09115"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU4848346.1"
                     /translation="METVGQQLIIAAIPTLPALVFFVLVLTGRLLRENAQYIAILGVT
                     VSLVFSVFALLYVAGIIPGGGKPIAFSVNWIDLGEGGSFPMGVYIDGLAAVMLVVVCF
                     VSLMIQLYSGGFMEGDSRFAWYYAVLNLFTASMLGLVLAPNFIELYVFWELVGLCSYL
                     LIGHWYEKPSARDAALKAFIVTRIGDAGLFIAIIVFWAYTGTTSFDGISDAAQAGFIG
                     GSVFTTAVVLVFIGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVART
                     YNIFVQSEQAMLVVAYTGGFTALMAATMALVKKDIKRVVAYSTVSQLGYMMMGLGIGS
                     YTASMFHLYNHAFFKALLFLCAGSIIYAMDSYNLFEMGGLRQRMPITFWTMVIGGLSL
                     AGIFPLSGFWSKDAIVASAYDEHYYVLFAMALITVFLTAFYIFRAIFLAFTGEPRTEG
                     ARAAVESPGIMLGPMVILAFLSVVSGWVGIPSGFGIPVKDYFAELVAPSSFASETLGL
                     EAHPFSLVLAAVSFLAALIGIGLAYALYVQRPELAGALSRRFSGLYTFLDKGWYFDAL
                     YGATFVRAAMYLGRLAREFDRRVLVGGLTGGVGRSVSGFGELLRRLQTGGVQNYALFI
                     LLSVLIIGIIAGAQYAFLVFAIIALITITAFAVGSRL"
     gene            7044..8498
                     /locus_tag="VFT03_09120"
     CDS             7044..8498
                     /locus_tag="VFT03_09120"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU4848347.1"
                     /translation="MITTVIIFIPLLAAILMLLLPNDARLARYAALGVAAVPLLLATY
                     VYFAFGGNLDAAPLSQDATWIESLNVGYRVGLDGLGFGMFWLTTLLTLIAIVTSWDVR
                     DNLKQYFAILFIAELGMLGVFAAQDLILFYVFFEITLIPMYLLVGIWGDENRRQAAIK
                     FFIYTFLGSTVMLAGFLAFGILADSFAMASLNAGGGLSRTAQVAIAAPILFGLLVKVP
                     AVPLHGWLLDVYVSSPISTNVVLSGILPKLGTYGLIRIAIPLLPQGVEPFLPFLAILG
                     VVNILYGSFVAFLQPDLKALVAYSSIGTLGFILLGAASGNAVGLNGAVFQQVSHGLYS
                     ALLFILVGVIAARTGTRRISELGGLASRMPWAGGLLALGALAAMGLPGLAVFVSEFLS
                     IMGGYESFPVQGVLAALGIIFSAMYLLYMLARVIFGPIERPAYEGISDAGPVEMAAIV
                     PLAALLLVLGIFPALLISVQRPAVEAILSAIGGS"
     gene            8498..9925
                     /locus_tag="VFT03_09125"
     CDS             8498..9925
                     /locus_tag="VFT03_09125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU4848348.1"
                     /translation="MVTGETFLALLPELVATGFLILVLMVGVFAERNIGLVAGLAALG
                     SLAVFGSAAVQLAIGISGSFFGGGYVVDGFALYFKLIVAGSAFFAILAAARWSGYTGD
                     EGEYLTLILSVVLGSLLLVSMRDLFGLFLAIELATIPSYAMVAFDRSRRESAEGGMKY
                     LITGVIASSVLLYGIVLIYGVAGSASLTDVAEAFSGGLSPVAVLGLVLLISGFAFKVS
                     AAPFHFWTPDAYQGAPTSAAAFLSVAPKAAFFGALLRVLLEGMPEAALTWTAVMAFLS
                     ILTMFVGNLFALRQSNVRRMLAYSSVAHSGYILAAFAALQGQAVATAVEAILIYSAAY
                     AVMNLGAFLVIDLVGEDTKSFNGLIRTSPGVALSMGVFMASLVGIPPFSGFFGKAWII
                     LAGAQSGSVLVYVVVGALVVNSVLSVPYYFGIIRNMFFEEPAGPAKDGAGAVKLSVYA
                     LALATTLFGLLIIPLSALVQASGLL"
     gene            10035..11141
                     /locus_tag="VFT03_09130"
     CDS             10035..11141
                     /locus_tag="VFT03_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEU4848349.1"
                     /translation="MQAVILVGGLGTRLRPITYDVPKALVPLRNRPFMGYMLDFLRNG
                     GLDGAVLSLGYLPTPIQEYLAGFDLAGFSVDYAVEEHALGTAGGIKNAARFLREGGSV
                     VAVNGDVLSGTDLRSLIERHKETDALATITLTSVEDPTAYGLVEVDHDMVVRRFIEKP
                     AADEVTTNLVNAGIYVLEPEVLEMIPPGREVSIEREIFPELQALGRLRAHVSSSYWRD
                     IGTPRSYLAACHDVLSGAVGATREGFEYCDVDGSVRKGKNVKLLPPVSVAAGCEIDSL
                     ATIGGRSALGRGCRVGAGAVIEGSILLEGAEVEAGAVVRGSIIGKGARVGQDAIVRGL
                     SVLGAGCVVGEGNVLDQGIRVNPGVVLPPRSLRF"
     gene            11288..11983
                     /locus_tag="VFT03_09135"
     CDS             11288..11983
                     /locus_tag="VFT03_09135"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU4848350.1"
                     /translation="MPETSNPYLSALADLLFPQRCVGCERRASDLLCRECFEALPRIG
                     RPVCARCGMPTAFETFVCDECKGMDLGFESARAPLRYEGVGEEIVHALKYRGYTRVVD
                     RLAVPLMLGVLDRGERFDAVVPVPLHRSRLRRRGFNQAELLARGVAAGLNAPFSDTLR
                     AVRKTRDQVELSAADRRANVAGAFAVRERARGRILLVDDVFTTGATTSECAESLLAGG
                     AKEVHAVSLCRTV"
     gene            12224..12790
                     /gene="raiA"
                     /locus_tag="VFT03_09140"
     CDS             12224..12790
                     /gene="raiA"
                     /locus_tag="VFT03_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU4848351.1"
                     /translation="MDVTVKGRNISVTEALERYAFEKVERVSKFFDENRSASRAEVEL
                     IHERNPSIPDPEIAEATLFINGTVLKAREASQDMYASIDRMSDKLERQVKRYRGRQID
                     RWHGQMKNSHVEPPIEPEPFVVEEEEEARIVRTKQFQMKPMSAEEAVLQMELLDHDFF
                     VFTSADTGDINVVYRRRDGDYGLIEPAR"
     gene            12877..15474
                     /gene="secA"
                     /locus_tag="VFT03_09145"
     CDS             12877..15474
                     /gene="secA"
                     /locus_tag="VFT03_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU4848352.1"
                     /translation="MANLLTKILRMGEGRKVKALQQRVEAVTALEPEMEKLSDGALRA
                     KTDEFRERLSESETLDDILPEAYAVVRETSRRTLGMRPFDVQVMGGIALHQGKIAEMK
                     TGEGKTLAATMPVYLNALSGKGVHIVTVNDYLARRDAGWMGEIYEFLGLKVGLIQDNM
                     GFEERKVAYDSDLTYGTNAQFGFDYLRDNIATSTDQLVQRELSFAIVDEVDSILIDEA
                     RTPLIISGMPESAADVYYRFAAIIPRLREGEDYEVDEKKRQIAPTESGVEKVEKALGV
                     ENLYDDVNTNLVNHLNQALRAHALFHKDVEYIVSDGQVFIVDEFTGRVLEGRRYSEGL
                     HQAIEAKESVEIREENQTVATITIQNFFRQYDKLSGMTGTAATEADEFMHIYKMEVVS
                     IPTHKDMIRDDRDDLVYKTKKAKYGAVIDDLVERNSAGQPVLVGTVSVDVSEHLSGLL
                     KRRGIRHNVLNAKQHEREAEIIAEAGERGAVTIATNMAGRGTDIKLGPGVAELGGLYV
                     LGTERHESRRIDNQLRGRSGRQGDPGESRFYLSFEDDLLRLFGGDRMQGVIDRIGLED
                     DVPIEAGMISNSVRRAQEQVESRNFQIRKRILEYDDVLNKQREVIYAIRRDILMGEDV
                     DTMSYVEEVLTEVVSQYVSGDVYPEEWDLETLAAEINRFYPTTIDFKALDVENLSSED
                     VLELVLADARERLEERRAEWEERTAELERRGLARTDGVASFEEAERRTLLSVVDSRWR
                     EHLYEMDYLREGIGWRGLSQRDPLVEYKREGFDIFQEMERGLKEDYVTYIYRIENVML
                     REEEMQQLSYSGGGEEPNQQPKSPRSSEGKVGRNDPCPCGSGKKYKKCGLLNTPEHQR
                     LTSQGGSAA"
     gene            15474..>16016
                     /locus_tag="VFT03_09150"
     CDS             15474..>16016
                     /locus_tag="VFT03_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU4848353.1"
                     /translation="MSELKDRAAELEERLAELEAFFDVEGLREEAAELGEQMSRPGFW
                     DDQERARELSARFSRGQGRIGLLEGLRGSLSDSEELLELADEDGDLVSEVEEELRRVE
                     RVLEEQEVARLFTGEYDEGDAILTINSGAGGVDSQDWAEMLSRMYRRWAERRGFEVEV
                     IEYTEGEEAGIKSATFTVSGE"
BASE COUNT         2742 a         4912 c         5374 g         2988 t
ORIGIN      
        1 gccgccttcc tctatccctg ggcggtcatt taccgtgagc ttggtttgta tggattcgtc
       61 gagatggtga tatttgtgtt gatcctgggc gtcggcctcg cctacgcctg gaagaagggg
      121 gccctcaagt gggtgtgatg cagcagttca acgagccgaa cctcataacg acgagctcgg
      181 acaagctctt taactgggct cgcaagaact cgttgtggcc gctgcagttc gggctggcct
      241 gttgcgctat agagatgatg tccacgggga tggcgcacaa cgacctcgac cgcttcggtg
      301 cggggttctt ctcggcctcc cccaggcagg ccgacgtcat gatcgtctcc ggcaccgtct
      361 ctaccaagat ggccgagcgg atgacgcggc tctacgagca gatgcccgag cccaagtggg
      421 tcatagcgat gggggcgtgt gccatcagtg gcggcccttt cctcgacggc tactcggtgc
      481 tcatgggggc agacagggtg atcccggtcg acgtctacgt gccagggtgc ccgccgagac
      541 ccgaggcgct catcttcggc atcatgacgc tccagaagaa gatcgagcgc gaccagcagg
      601 tcggctacga gaagccccgc cccgcgctcg tgaacgagga cggctccata aacgagtacc
      661 tcccggagcg tgagtaccag cacatcgccg agggcaaggc cccgcgcttc gagggcataa
      721 ggccgctcaa gcgcacctac gtcttcgagc gcaaggggat gccgcccgcc tcccagactg
      781 atgtgggctg gatccccgca gaggccaaga agaagctcaa ggagaaggag aaggccgaga
      841 aggccgcgca ggagaacggc ggggctggcg gcaaagccga ggcgagcgcc gaggcgaacg
      901 gcaagtccgg gggcgaggca aaagccggta ctgacggcgc gaagaaggag gatgcctgag
      961 gtggcggtga cgttgccgga gggagatctg gagacacgcc tggagcgcta cctcgagggg
     1021 ctgcgcaacc gccatgggtt ggaagagtcg ggtatcgaag gcaacgtggt gcgggttctc
     1081 gtggataccg agtcgttgcc agcggtgctt gcgacggcgc gggaggcttt agggatactg
     1141 cacctctcat tcatcactgt cgtggacagg gagggctcct tcgagctgac ctacgagctc
     1201 ctggactttc gcggagcgcg tgtgagggtc aagacggtcg tggagggcac ggagccggtc
     1261 gttcccacgg ttacgaacgt ctacccgacg gcgaactggc acgagcggga ggcctacgac
     1321 atgttcggga tcgccttcag ggggcaccct gacctgcggc ggacgtacat gtggcaggat
     1381 cacttcgacc accatccgtt gctcaagagc ctggagatca gcaccaagcg tacgttcgag
     1441 ggattgaggt agaagatgct tggcgaagag cgccgcacgg accatagcgc gcttacggag
     1501 ccggcgagcg gccggctcgt caaccccgac gtccgggacg agttcgggct cgagaccata
     1561 gacatgagca tgggcccgca gcaccccgcg atgcacgggc tcttgcggct catcctggag
     1621 ctcgacgggg agaccgtggt gcggtgcgac cccgtcatgg ggtatctgca ccggtgccag
     1681 gagaagatcg ccgagaaccg catgtaccct gcggttatcc cgctgaccga cagactcgac
     1741 tacttcgcca atatgcacaa cgagcacggc tacgtcctcg cggtcgagga tcttctggac
     1801 gtggagatcc cgccccgggc ggagtacatc agggttctca tgtgcgagct catgcggctg
     1861 gcgagccaca ttccctccat agggttccta ctcctggagc tcggggcctt caccccgatc
     1921 ctgtacgcct tcagggagcg ggagcggata cagagcatct tcgaggcggt cacgggcgcg
     1981 cggatgatgt tccactacat ccgcatagga ggggtcaagg ctgaccttcc gccggaaata
     2041 ccagagaaga tctgggagtt tgtcgaggga ctggagaaga acttccagtc ggacttcgtc
     2101 gacctcatcg aggggaacga gatcttcatc tcccggacgc ggaacgtcgg gaagatgacg
     2161 caggagcagg cagtcgagat gggcctgacc gggccgcccc tgcgctgtac cggcgtggac
     2221 ttcgacatca ggctcgacta tccctacagc gtctaccccg agctgaagtt cgacgtcgta
     2281 accgacacgg gcggagacgt tgaggcatcg taccgggttc ggatcgggga gtgcctggag
     2341 agcgtcaaga tcatcaagca ggtcctggag aagatgccgg aaggcgcggt tatggccgac
     2401 atgggacgcc gcatcaggcc gcccgagggc gagggcttcg ggcgcgtcga gagcccgagg
     2461 ggcgagttcg cggtgcacgt cgtctccgac ggttcggaca cgccgtatcg ggtgcactac
     2521 cgcgacccga gcttcgtcaa catgcagctc ttgcaggaga tagtgccggg gcactacctg
     2581 cccgacataa tgcccatcat gggcctcgtg gaccctgtca gcggcgggtg ggacaagtag
     2641 tggggaccgt cctccagtac ctgaaccagg agccattgag gttcctcata tccttcggtc
     2701 tgatactctt tctcgtcctc aacctggccg ccgtcctgac gatggccgag cgcaaggtgt
     2761 cggcgtacat ccagctgcgt tacgggccga accgggtggg gccgcggggg ctcttgcagc
     2821 ccgcggcaga cgtgttcaag ctgttcacca aggagaacgt ctctccggga cggtccgacc
     2881 tctgggtctt cctcggcgcg ccgatcgcca tgttcctgcc cgcggctctc gtatggctcg
     2941 tcataccctt cgcccccgac gcggtgatgg ccaacctgaa cgtcgggatc ctgttcttca
     3001 tagctgtgac gtccatcggg gctctgggcg tggtgatggc aggctacggc agccgctcca
     3061 ccttctcgct gctcggcgct ctcaggggtg cggcgcagat gatctcctac gaggtgccgc
     3121 tcatcttgag cttgctcggg gtcgtgatgc tcgcggggag cctctcgttc gtggacgtgg
     3181 tgctcgccca gggcgacacg ttctggacct ggtattttgt gccgctgttc cccatgttca
     3241 tcgtcttcta cgtatcaggg atcgctgaag tcaagagggt tccgttcgac ctgccggagg
     3301 gggagagcga gatcgtcggc gggttcatga tcgagtactc ggggatgacg tgggcgctca
     3361 tccaggcggc ggagtttgcc tcgatgggtc tgatgagcgc tatcaccgct acgcttttcc
     3421 tcggcggctg gcagccgccg ctcccattcc tcgacctcgg ggcgttcaac tgggtctggt
     3481 tcgggctcaa gacggcgctc atcatgttca ccttccagtg gatccggtgg agcgtcccca
     3541 ggctcaggat ggaccagctc atggacttcg gctggaaggt gctcgtcccg ataacgctca
     3601 tctatctctt catcgtcgcc gcggcgatgc ttatcatcta ggaggttctc taacgtgccg
     3661 caggtaggac aggggattct gaaggggatg ggggtaaccc tcaagcacct cttcgggcag
     3721 aagatcacac gccagtaccc cgagtacaag cgcccgctac cagagcgcag ccgcgggatg
     3781 ctgaccgtgg acatggacag gtgcatcgcc tgcctgcagt gcatgaggat ctgccccgac
     3841 cactgcatct ccatcgagca ggagaagcgg gacgccgacg gctcgggcaa agcgaagccc
     3901 tacgccgtgg gcttcgttat cgacgactcg cggtgtatgt actgctcgct ttgcgtcgag
     3961 gtctgcccgg tcaactgcat ctaccacacc gaggagttcg aggcccaggc gtacaaccgg
     4021 ctcgagctcg cgcggcagtt cggcgagcgg cccgtagacc cgaccatcga tcccaagccg
     4081 accgtgaaaa agaagaaaaa gcccacaggg gaaaaggctc ccaagacagt agagcgtccc
     4141 agaaccggaa aggccgaggc ggtttgaccg tcgcgttcgc tttcctggcc gggatgacgc
     4201 tcgtctcggc gttaggggta gtcctgagcc gcaacgtcgt gcactcggcg ctgttcatgg
     4261 cgggctcttt cttaggtgtc gcggggtact tcgtaatgct caatgcaccg gcgctcgcgg
     4321 cgttccaggt cctcatctac gtcggggctg tgacggtggt gatcctgttc ggcatcatgt
     4381 tcacccagaa gccccaggtc aggcgctacc gcacgatcat caaccgccag agctggtggg
     4441 gcttcttcgt ggcggctggc gtcgggacct tcctcgccta cgtcctcgcg aacgaggact
     4501 ggggctctac gtcgcccggc cacggaccgc gcgacgtggg agccttcggg cgggacctgc
     4561 tcggcgtgag cgcggggtcg gaggtcttcg tgcttctctt cgaggtcgcc tccgtgctgt
     4621 tgctggtcgc gatcgttgcg gccatcgtca ttagccgccg caagacgggc gagggtatcg
     4681 accggggggt gcgtgagtaa tgccaccgga ggcgcaacag atcctggacg ccctcgtggc
     4741 cgcgccaccg ctcgagggat acctgatagt cggagcgctc ctgttcgcca tcggggcgtg
     4801 gggcgcgctg gtcaggcgca acgcggtcgt cgtgttcatg tgcgtggagc tcatgatcaa
     4861 cggcgtcaac ctgaccctgg ttgccttcgg cgattatctg ccgaacgcgg ggggtttggg
     4921 ggtgagctac gcggtgctcg tgatagctat cgccgccgcg gagatcgcgg tcggcctcgc
     4981 tatagttctt accgtgttca ggacgcgccg gaccgtgaac ttggacgaag tcacctcgat
     5041 gaggggctag ataggggtta gatggagacc gtcggccaac agctgatcat cgcggccatc
     5101 cccactctgc cggcgctggt cttcttcgtg ctcgtcctca ccggacgcct cttgagggag
     5161 aatgcccagt acatcgccat cctcggggtt accgtctcgc tggtcttctc ggtcttcgcg
     5221 ctgctctacg tcgcgggtat catccctggc ggcggaaagc cgatcgcgtt ctccgtcaac
     5281 tggatagacc tcggagaggg cggctccttc ccgatggggg tctacataga cggtctggcg
     5341 gccgtcatgc tcgtcgtcgt gtgcttcgtg agcctcatga tccagctcta ctcgggcggg
     5401 ttcatggagg gcgatagtcg cttcgcgtgg tactacgccg tgctcaacct gtttaccgcc
     5461 tcgatgctcg ggcttgtcct cgcgcccaac ttcatagagc tctacgtctt ctgggagctc
     5521 gtgggcctct gctcgtacct cctgatcggg cactggtacg agaagccatc ggcgagagat
     5581 gcggccctga aagcgtttat cgttactcgt atcggcgacg cggggctctt catcgcgatc
     5641 atcgtcttct gggcgtacac cggcacgacg tccttcgatg ggatctccga tgcggcgcag
     5701 gccggtttca tcggcggctc ggtctttacc acggctgtcg tgctggtctt tatcggggcg
     5761 gtgggcaaga gcgcgcagtt cccgctgcac gtgtggttgc ctgacgcgat ggagggcccg
     5821 acgccggtct cggccctgat ccacgcggct accatggtgg ccgcgggcgt ctaccttgtg
     5881 gcgcgcacct acaacatctt cgtccagagc gagcaggcga tgctcgtggt ggcgtacaca
     5941 ggcgggttca cggcgctcat ggcggcgacg atggcgctgg tcaagaagga catcaagcga
     6001 gttgtcgcct actcgacggt ctcgcagctc gggtacatga tgatgggcct tgggatcggc
     6061 tcgtacacgg cgagtatgtt ccacctctac aaccacgcct tcttcaaggc gctcctgttc
     6121 ctgtgcgccg gtagcatcat ctacgcgatg gactcctaca acctcttcga gatggggggc
     6181 ctgcgacagc ggatgccgat caccttctgg accatggtga tcggcgggct ctcgctcgcg
     6241 ggtatcttcc ctctgagcgg gttctggtcg aaggacgcga tagtggcctc agcctacgac
     6301 gagcactact acgtcctgtt cgcgatggcc ctgattacgg tgttcctgac cgctttctac
     6361 atctttcggg cgatcttcct cgccttcaca ggcgagccgc gcaccgaggg agccagggcg
     6421 gcggtcgaat cgccggggat catgctagga ccgatggtta tactggcctt cctctcggtg
     6481 gtgagcggct gggtcggcat cccgagcggg ttcgggatac cggtaaagga ctacttcgcg
     6541 gagctcgttg ctcctagctc gttcgccagc gagaccctgg ggctggaggc gcacccgttc
     6601 agcctggtgc tggcggcggt ctccttcttg gcggcgctca tcggcatcgg gcttgcctac
     6661 gccctctacg tccagaggcc ggagctggcc ggggctctct cacgccggtt ctcgggcctc
     6721 tacaccttcc tggacaaggg ctggtacttc gacgcgctct acggcgcgac gttcgtgcgg
     6781 gcggcaatgt atctggggcg tctcgcgcgc gagttcgacc ggcgagtcct cgtcggcggg
     6841 ctcaccggcg gcgtcgggcg ctccgtatcc gggttcgggg agctgctcag gcggctacag
     6901 accggcgggg ttcagaacta cgcgttgttc atcctgctca gcgtcctgat catcgggatc
     6961 atcgctggcg cgcagtacgc gttcctggtg ttcgccatca tcgcgctgat caccatcacc
     7021 gcctttgctg tggggtcgcg cctgtgatca cgaccgtcat catcttcatc ccgctgctcg
     7081 ccgctatcct gatgctgctc ctcccaaacg acgcgcgact cgcgcgctac gcggccctcg
     7141 gcgtcgcggc cgtgccgctg ctgctcgcga cgtacgtcta cttcgcgttc ggggggaacc
     7201 ttgatgcggc gccgctctcg caggacgcga cctggataga gtcgctcaac gtgggctacc
     7261 gcgtcgggct cgacggcctt ggcttcggga tgttctggct aacgaccctg ctgacgctca
     7321 tagccatcgt gacgtcgtgg gacgtgcggg ataacctcaa gcagtacttc gccatcctgt
     7381 tcatagcgga gctcgggatg ctcggggtgt tcgccgcgca ggacctgatc ctcttctacg
     7441 tcttcttcga gatcacgctg atcccgatgt acctcctcgt gggcatctgg ggcgacgaga
     7501 acaggcgcca ggccgctatc aagttcttca tctacacctt cctgggctcg acggtgatgc
     7561 tcgccggctt cctcgccttc ggcatcctcg cggatagctt cgcgatggcc tctttgaacg
     7621 cgggcggcgg tctctcgcgc acggcgcagg tcgccatcgc ggccccgatc ctcttcgggt
     7681 tgctggtcaa ggtccccgct gtgccgctgc acggctggtt gctcgacgtg tacgtctcca
     7741 gcccgatctc gacgaacgtg gtgctctcgg gcatcctgcc caagctcggc acctacggcc
     7801 tgatccggat agccattccc ctcctccctc aaggggtgga accgttcttg ccgttcctgg
     7861 ccatcctcgg cgtcgtgaac atcctctacg gctccttcgt cgcgttcctg cagccagacc
     7921 tcaaggcgct cgtcgcctac tcctcgatcg ggacccttgg cttcatcctg ctcggggcgg
     7981 cgtcgggtaa cgcggtcgga cttaatggtg ctgtcttcca gcaggtctcg cacgggctct
     8041 actcggcgct gctgttcatc ctggtcggcg tgatcgccgc ccggaccggg acgcgccgca
     8101 tctccgagct cggcggcctc gcatccagga tgccgtgggc cggaggcttg ctggctctgg
     8161 gcgctctggc cgcgatgggg ctcccggggc tcgcggtctt cgtctccgag ttcctaagca
     8221 tcatgggcgg ctacgaatcg ttcccggtac agggagtgct ggcagccctg gggataatct
     8281 tctccgcgat gtacctgctc tacatgctgg cgcgcgttat cttcggcccc atcgagcggc
     8341 ccgcgtacga agggatctcc gacgccggtc cagtcgagat ggcggcgata gtccctctgg
     8401 ccgcgctgtt gctggttctc ggcatctttc cggcgctgct cataagcgtc cagcggccgg
     8461 ccgtggaagc catcttgagc gcgatagggg gtagttagtg gtaacgggtg aaaccttcct
     8521 cgcgctcctt ccggagctcg tcgcgaccgg gttcctgatc ctcgtcctca tggtcggggt
     8581 gttcgccgag cgaaatatcg ggctcgttgc ggggctggcg gctctgggct cgctggctgt
     8641 gttcggaagc gcggctgtgc agctcgcgat cggcatctcg ggcagcttct tcgggggcgg
     8701 gtacgtggta gacggtttcg cgctgtactt caagctgata gtggccggct cggcgttctt
     8761 cgcgatcctg gcggcggcgc gctggtcggg gtacacgggc gacgagggag agtatctgac
     8821 cctgatcctc tcggtcgtgc ttggctcctt gctgctcgtc tcgatgcggg acctgttcgg
     8881 tctcttcctc gctatagagc tcgcgacgat cccttcgtac gccatggtcg ccttcgaccg
     8941 gagcaggcgc gagagcgccg agggtgggat gaagtacctg atcacgggcg tcatagcctc
     9001 ctccgtgctc ctctacggga tagtcctgat ctacggcgta gccggctcgg ccagcctcac
     9061 tgacgttgcg gaggcgttct ccggcggcct ctcccctgta gcggtgctcg ggctggtgct
     9121 cctaatctcc ggcttcgcct tcaaggtctc cgcggctccc ttccacttct ggaccccgga
     9181 cgcctaccag ggcgccccca cgagcgctgc cgcgttcctc tccgtcgcgc cgaaggccgc
     9241 cttcttcgga gctctcctgc gcgtcctcct ggagggaatg cccgaggccg cgctgacctg
     9301 gactgcggtc atggcgttcc tgtccatcct gacgatgttc gtcggcaacc tcttcgcgct
     9361 gcggcagagc aacgtgagga ggatgctcgc ctacagctcg gtggcccact cagggtacat
     9421 cctcgcggct ttcgcggcgc tgcaagggca ggcggtcgcc acggcagtgg aggctatcct
     9481 gatctactca gcggcctacg cggtgatgaa cctcggcgcc ttcctcgtaa tagacctcgt
     9541 cggcgaggac acaaagagct tcaacggctt gatcaggacc agccccggcg tcgccctgag
     9601 catgggcgtc ttcatggctt ccctcgtcgg gataccgccg tttagcggct tcttcgggaa
     9661 ggcctggatc atcctggccg gagcccaatc aggctcggtg ctggtctacg tggtcgtcgg
     9721 ggcgctcgtc gtcaacagcg tcctctccgt cccgtactac ttcggcatta tccgcaacat
     9781 gttcttcgag gaaccggcag ggcccgcgaa ggacggagcc ggagccgtga agctctccgt
     9841 gtacgccctc gctctcgcca ccaccctgtt cggcctcctc atcatccccc tctccgcgct
     9901 ggtccaggct tccgggctgc tgtaacccgc cacggcggag ctggaggctt tgctttcggt
     9961 ccacctttat ctgtagtaca ggtgaagact ccaccgtagc ctgagccggg tggatagtgt
    10021 aatctgcggc tcatatgcaa gccgttattc ttgttggggg gctgggcact cggttgcggc
    10081 cgatcacgta cgacgttcct aaggcgctcg tgcctttgcg gaaccggccg ttcatgggct
    10141 acatgctgga tttcctgaga aacggcggcc tcgacggggc tgtgctctcc cttggttacc
    10201 tgccgacccc catccaggaa tacctcgccg ggttcgacct ggcggggttc tcggtggact
    10261 acgcggtcga ggagcacgcg ctcgggaccg cagggggtat caagaacgcc gcgcggttcc
    10321 tgagagaagg gggctccgtg gtcgcggtga acggcgacgt gctctcggga acggacctga
    10381 ggagtctcat cgagaggcac aaggagacgg acgcgctggc gacgattacg ctcaccagcg
    10441 tcgaggaccc gacggcctac ggcctcgtcg aggtagacca cgacatggtg gtgcgccgct
    10501 tcatcgagaa gccggccgca gacgaggtca cgacgaatct ggtgaacgcc gggatctacg
    10561 tgctggagcc ggaggtcctc gagatgatac cgccaggccg cgaggtctcg atagagcgcg
    10621 agatcttccc cgagctgcag gcgctgggcc ggctgcgggc gcacgtctcg agctcctact
    10681 ggcgggacat cgggacgccg cgcagctacc tcgccgcctg ccacgacgtg ctctccgggg
    10741 cggtgggggc aacgcgcgag gggttcgagt attgcgatgt ggacggctcg gtcaggaagg
    10801 gcaagaacgt gaagctcctc ccgccggtat cggtggcggc ggggtgcgag atcgatagcc
    10861 tcgcgacgat aggcgggcgc tccgcgctgg ggcgcggctg ccgcgtgggg gcaggcgcgg
    10921 tgatcgaggg cagtatcctg ctcgagggcg ccgaggtcga ggctggagcc gtcgtgcgcg
    10981 gctcgatcat cggtaagggg gcgagggtcg gacaggacgc catagtccgc ggcctatcgg
    11041 tgctcggggc tggttgcgtc gtaggcgaag ggaacgtcct tgaccagggg atccgggtga
    11101 accccggggt cgtgctgccg ccaaggagtc tgcgattcta gacaggtttc aggctacagg
    11161 catcaggctt caggttttcc ttctcgcgcc cgatatcttg cactggtaag acaagctggg
    11221 atcgcgccgt cgtggttacg gctgcggacg gcgagaattg gcgtccgccc ctgaaacctg
    11281 acacctgatg cctgagacct cgaaccctta cctttcggcg ctcgccgacc tcctcttccc
    11341 gcagcgctgc gtcggctgcg agaggcgggc gagcgatctc ctctgccgcg agtgctttga
    11401 ggcgctgccc cggatcggcc gcccggtgtg tgcgcggtgc gggatgccta cggccttcga
    11461 gacgttcgtg tgcgacgagt gcaaagggat ggacttgggc ttcgagagcg cccgcgcgcc
    11521 cctgaggtac gagggcgtcg gcgaggagat cgtgcacgcc ctcaagtaca ggggctacac
    11581 gagggtagtg gacagactcg ccgtgccgct catgctcgga gtcctcgacc gcggcgagcg
    11641 cttcgacgcg gtcgtccccg ttccgctgca ccgctcgcgg ctcaggcgcc gcgggttcaa
    11701 ccaggcggag ctcctcgcgc gcggcgtcgc tgcaggactg aacgcaccct tctcggatac
    11761 gctgcgagcc gtgcgaaaga cccgggacca ggtcgagctc tccgcagccg acaggcgggc
    11821 caacgtcgcg ggagccttcg cggtacggga gcgtgcgcgg ggtaggatac tgctcgtgga
    11881 cgacgtcttc accaccggag cgacgacgag cgagtgcgct gagagtctcc tggcgggcgg
    11941 cgcgaaagag gtgcatgcgg tgagcctgtg caggacggtg tagggcttga gcttgcgatc
    12001 tcgaagttgt gccggtagcc ggctctggac gctccttgtg agcgcaggag atcgcgtatc
    12061 ggataaacta gaagcggtga gcgaaactcg aggtccgagc ggcgcgggag cctgcgcggg
    12121 ccggggtagc gtatgagctg gggctcgaga gcctgtcggt gtgccggcaa gccgaaacgc
    12181 tgattagctg atcagccaca tacctgaccg aagggaggcg cacgtggacg tgacggtcaa
    12241 ggggcgcaac atctcggtga cggaggcgct ggagcgctac gcgttcgaga aggtcgagcg
    12301 cgtcagcaag ttcttcgacg agaaccgaag cgcttcccgg gctgaggtcg agctcatcca
    12361 cgagcgcaac ccttccatcc cggatcccga gattgccgag gcgaccctgt tcatcaacgg
    12421 gacggtcctg aaagcgcgcg aggcctcaca ggacatgtac gcctccatag accgcatgtc
    12481 ggacaagctc gagcgccagg tcaaacggta cagggggcgc cagatagacc gctggcacgg
    12541 ccagatgaag aactcccacg tcgagccgcc catagagccc gaacccttcg tcgtcgagga
    12601 ggaagaggaa gcccgcatag tccgcacaaa gcagttccag atgaagccga tgagcgcgga
    12661 ggaggcggtc ctccagatgg agctcctcga ccacgacttc ttcgtcttca cgagcgccga
    12721 tactggcgac ataaacgtgg tataccggcg gagagacggc gactacggac tcatagagcc
    12781 cgcaagatag ctccggatac cttaaactat aggggatggc ggaccgcgca cgggcggccg
    12841 tcgcgaggtg cgttgatctg aggttggtgt ttatcattgg ctaatctgct aacgaagata
    12901 ttgaggatgg gtgagggccg gaaggtgaag gccctccagc agcgcgtcga agcggttacc
    12961 gcgctcgagc cggagatgga gaagctctcc gacggcgcct tgagggcgaa aacggacgag
    13021 ttccgcgaga ggctgagcga gagtgagacc ctcgacgaca tcctgcccga ggcgtacgcc
    13081 gtcgtgcgcg agacctccag gcgcacactt gggatgcgac ccttcgacgt gcaggtcatg
    13141 ggcggtatcg ctctgcacca gggtaagatc gccgagatga agacaggcga gggcaagacg
    13201 ctcgctgcga ccatgcccgt ctacctcaac gccctgagcg gcaagggcgt ccacatcgtt
    13261 accgtcaacg attacctcgc caggcgcgac gcaggctgga tgggtgagat ctacgagttc
    13321 ctcggcctca aagtaggtct cattcaggac aatatgggtt tcgaggagcg caaggtagcc
    13381 tacgactcgg acctcaccta cggcaccaac gcccagttcg gcttcgacta cctcagggac
    13441 aacatagcga cgtccacaga tcagctcgtg cagcgcgagc tcagcttcgc catcgtggac
    13501 gaggtcgact cgatcctgat agacgaggcg cgcacgccgc tcatcatctc cgggatgcct
    13561 gagagcgcag ccgacgtcta ctaccgcttc gcggctataa tcccgcgtct cagggagggc
    13621 gaggactacg aggtagacga gaagaaacgc cagatcgcac cgaccgagag cggtgtcgag
    13681 aaggtagaga aggcacttgg cgtcgagaac ctctacgacg acgtcaatac caacctcgtc
    13741 aaccacctca accaggccct cagggcgcac gcgctcttcc acaaggacgt cgagtacatc
    13801 gtgagcgacg gtcaggtctt tatcgtagac gagttcacag ggagggttct cgaggggaga
    13861 cggtactccg aggggctcca ccaggccata gaggccaagg agagcgtgga gatccgggag
    13921 gagaaccaga cggtcgcaac catcactatc cagaacttct tcaggcagta cgacaagcta
    13981 tccgggatga cgggcacggc ggccaccgag gccgacgagt tcatgcacat ctacaagatg
    14041 gaggtcgtct ccatcccgac gcacaaggac atgatccggg acgacagaga cgacctggtc
    14101 tacaagacca agaaggccaa gtacggagcc gtcatcgatg acctggtcga gcgcaacagc
    14161 gcgggccagc cggtcctggt gggtacggtc tctgtggacg tctccgaaca cctctccggg
    14221 cttctcaagc ggcgcgggat caggcacaac gtcctgaacg ccaagcagca cgagcgggag
    14281 gcggagatca tcgccgaggc tggcgagcgg ggcgcggtca ccatcgcgac gaacatggcg
    14341 ggccgcggaa cggacatcaa gctcggtccg ggggttgcgg agctcggcgg cctctacgtg
    14401 ctcgggaccg agagacacga atctcgccgc atagacaacc agctcagagg ccgctccggg
    14461 cgccagggcg atcccggcga atctcggttc tatctgtctt tcgaggacga tctgttgagg
    14521 ctcttcggcg gcgaccgcat gcagggcgtc atagaccgca tcgggctcga ggacgacgtg
    14581 ccgatcgagg cgggcatgat ctccaactct gtcaggcgcg cgcaggagca ggtcgagagc
    14641 cgcaacttcc agatccgcaa acgaatcctc gagtacgacg acgtgctcaa caagcagcgc
    14701 gaggtgatct acgccataag gcgcgacatc ctgatgggcg aggacgtgga cacgatgagc
    14761 tacgtcgagg aggtcctcac ggaggtcgta tcacagtatg tctccggcga cgtctatccg
    14821 gaggagtggg acctggagac tctggcagcg gagataaacc gcttctaccc gaccaccata
    14881 gacttcaagg cgctcgacgt cgagaacctc tcgagcgagg atgtcctgga gctggtcctc
    14941 gccgacgccc gcgagcgtct ggaggagcgc agggccgagt gggaggagcg gaccgccgag
    15001 ctggagaggc gcggtctcgc gcgcaccgac ggcgtcgcgt ccttcgaaga ggcagagagg
    15061 agaaccctcc tctccgtggt cgactcccgc tggcgcgagc acctctacga gatggactat
    15121 ctgcgcgagg gcattggctg gcgcggcctt agtcaaaggg acccgctcgt cgagtacaag
    15181 cgcgagggct tcgacatatt ccaggagatg gagcggggcc tcaaggagga ctacgtgacc
    15241 tacatctatc gcatagagaa cgtcatgctg cgcgaggagg agatgcagca gctctcctac
    15301 agcggtgggg gagaggagcc gaaccagcag ccaaagagcc ccaggagctc ggagggcaag
    15361 gtcgggcgca acgacccgtg cccgtgcggt agcgggaaga agtacaagaa gtgcggactc
    15421 ctgaacacgc ccgagcacca gcgactcacg tcgcaggggg gctcggcggc ctagtgtccg
    15481 agctcaagga tagggccgcc gagctcgagg agcgcctcgc agaactcgag gcgttcttcg
    15541 acgtcgaggg gctgcgggag gaggcggccg agctgggcga gcagatgagc cgtcctggct
    15601 tttgggacga ccaagagagg gccagggagc tctcggcccg cttctccagg gggcagggaa
    15661 ggatcggtct tctggagggg ctgcgtggaa gcctctccga ctccgaggag ttgctcgagc
    15721 tcgcggacga ggatggtgac ctcgtctccg aggtcgagga agagctgcgg cgggtcgagc
    15781 gggtgctcga ggagcaggag gtggcgcgcc tcttcaccgg cgagtacgac gagggggatg
    15841 ccatcctcac gataaactcc ggcgccggcg gggtagactc gcaggactgg gccgagatgc
    15901 tctcccgcat gtaccggcgc tgggccgagc gcaggggctt cgaggtcgag gtcatcgagt
    15961 atacggaggg cgaggaggcc ggcataaaga gcgccacctt caccgtctcg ggcgag
//
LOCUS       DASTUV010000450         8334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_190503, whole genome shotgun sequence.
ACCESSION   DASTUV010000450 DASTUV010000000
VERSION     DASTUV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190503"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VFT03_09155"
     CDS             <1..1855
                     /locus_tag="VFT03_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEU4848354.1"
                     /translation="ASLDDRHVLGQVLELMPENWSGRLEVEAIVDGDVTNDGGVRHLT
                     DHQTRRVDEGLLLTTETVSKRIRLSFATGATLRERDGGELPSEDASTSTALVQRWSFD
                     AAQGRSYTMRKIVTVFTSRDDPDPDARAVEKLEEAVGTGMPTLLERSAQAWAARWDSA
                     DVEIYGDDDTQRKARFAIYHLIGCANPEDEYASVGARSLTGERYKGHVFWDTEIFVLP
                     FFVYTHPPTARALLMYRYHTLPAARERARAMGYRGALYAWESTDTGADVTPPFVYNSA
                     GERLEILTGLQEHHISADVAYAIWQYWRVTRDEAFFSNAGAEMMLEIARFWASRAERG
                     EDGRYHILKVIGPDEFHEHADDNAFTNLMAQWALRRGLETVEWLRKHHPDRWRELAGR
                     IGLDESELALWEEVAVGLVDNFDPETNLFEQHSGYFELEDIDLRDFEPRTKSLDAILG
                     WSRLTRSQIIKQADVIMLLFLLGDKYPREVHEANFRFYEPRTTHDSSLSPSFHALAAA
                     RAGDLETARRYFERAANLDLDFAHGVTAAGGVHIAALGGMWHAMVFGFGGMYVEDEGP
                     RFEPHVPEDWQTLHFSVLWRGLQLWVSTTGNVAEVQKVEKEPTRVRPGPPE"
     gene            complement(2078..2482)
                     /locus_tag="VFT03_09160"
     CDS             complement(2078..2482)
                     /locus_tag="VFT03_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848355.1"
                     /translation="MMCEVKYCRSDAGVAATLAFLKGVPPAADNAKREQDRGVATFYE
                     VKTDAVALLRAMQEYHERHDPGAPFSAGMRLAPYLAAGRAGLDPDTLRYERALRYLVR
                     EGVLVWEERVGTVPGIDFYRFTGKGLELLSEA"
     gene            complement(2619..3545)
                     /locus_tag="VFT03_09165"
     CDS             complement(2619..3545)
                     /locus_tag="VFT03_09165"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEU4848356.1"
                     /translation="MFGPHRSDARRCALLLPILLLLGALAACSSPDSVDRSVAASPEA
                     GGGGPRTADAAPEATVGVAAYAREKGDSSGVPFITRQEASGGSSYEADTVLAVRYGAH
                     QGYERVVLDLGTGDEPAASVPEWTLMSSTGSGLLRVTLPSMSRTAVSDGTLGDALLQS
                     FYVVRAPEGGMFVDIFARKSFAYRVLELSDPARLVVDFRSEGSDVRPPVVGGDTVLVE
                     PRSGAQIGDPLTVRGYSRNFEASNTIALEDSSGRAVIQRTVLSNDWTSTWGYFEATLD
                     LPPFGGKGTLRVGTESARDRTFDGVEVPVKGE"
     gene            3704..4663
                     /locus_tag="VFT03_09170"
     CDS             3704..4663
                     /locus_tag="VFT03_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU4848357.1"
                     /translation="MITPDDLRLAQRRVRGVALRTPLVPCPRGEESRLLYFKAENLQP
                     TGAFKLRGAYNKISSLSPEERRRGVVAHSSGNHAQAVAYAASALGVRALIVMPRGAPR
                     VKTDATAAFGAEILFVGDDSAERVRRAEELAAEHGYVLVPPYDDETLIAGQGTVGLEI
                     LDDLPDVETVLVPVSGGGLISGVAAAIKQSRPEARVFGVEPELAADAQASLRSGKLVE
                     LPAEQVGRTIADGLRVRKLGHAPFEHIRAFVDDIITVSEDEILEAMRRMALRTRLVAE
                     PSGAVTFAGYLFHRDELPATRLNVAVVSGGNVEPQLLARILAD"
     gene            complement(4751..5686)
                     /locus_tag="VFT03_09175"
     CDS             complement(4751..5686)
                     /locus_tag="VFT03_09175"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4848358.1"
                     /translation="MAERNEAAGRGGLWMLPGAEGLSGVRMRPMDDVDLERVRELRSV
                     VSWAADPRAFDLLRGMREARWTVADASDGSLAGMVGAVPLGRIGILCHLAVRDDHRNM
                     GLGVRLSAWAVAYLRSQGAEVIRLYSTRQAEGLYRSLGFRPRAPRTVYRLDKATRAGG
                     GEEVDGHRVGTLSFGDLPELYGLDHWSYGADRSALIFATLRLHPGGGLIARDSSGRIK
                     GYLLRSYSGRVDRIGPFVASIPDVARLLLAAALRAGVGSPVEVTVPGIVESPAHELFR
                     EFGFRGRSDRLQMELGEEGGVLRMEQYGTTPYLAT"
     gene            6065..6706
                     /locus_tag="VFT03_09180"
     CDS             6065..6706
                     /locus_tag="VFT03_09180"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU4848359.1"
                     /translation="MRATLQPGLTNRLTKYLRTTQQLIEEGRDAVSSKELGDFTGINP
                     VQVRRDLNAIGFSGTRGVGYQAYDLVEAVRNILGLRQTYNIALVGAGNLGSAIAASTI
                     LPKRGFVIHDVFDNDPEKIGRTVGNVVVKHIDELEKSVAEAEEIIGIIATPASSAQEV
                     ADLMVDANIRVILNYTDVLLHVPSHVDVHRIDPTAQLMHTLYYLTQAEGQEAS"
     gene            complement(6869..7084)
                     /locus_tag="VFT03_09185"
     CDS             complement(6869..7084)
                     /locus_tag="VFT03_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848360.1"
                     /translation="MLEGGRNDPRLERSYRLLAWRILAAKGGTGLTGLVARLAREASS
                     LEEYEATRERALGPILEGLERGENRDP"
     gene            complement(7155..7502)
                     /locus_tag="VFT03_09190"
     CDS             complement(7155..7502)
                     /locus_tag="VFT03_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848361.1"
                     /translation="MGNRRVALKPHASKIRRWVEEGRNDDWIAQELNTTPSSVQSFRS
                     RNSIYRRDPVRRGQLSEHPAVLDEVEEGILLRTDARNSEVFDREWRGLMRGSPDDLHV
                     IITQDRIYVEKTR"
     gene            complement(7624..>8334)
                     /locus_tag="VFT03_09195"
     CDS             complement(7624..>8334)
                     /locus_tag="VFT03_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4848362.1"
                     /translation="EDGYPRYRGSRGMSSVADHLARGLDVRTGERVVAVKIRDKVWEA
                     RTEAGSQVAGTALLLTAPVPQSLLLFEGGSALSEETRQRLAGITYSPCLALMALMNGP
                     TGVPEPGGIQIKGEPLDWIGDNQRKGISEAPAITIHAGPEWSRENFESEEALVAASLL
                     AFAAEQLATDLPSRVLETSLVRWRYSWVTQPHPEPCFVASGDPPLLFAGDAFGPSKVE
                     GAALSGLAAADHLSTAVD"
BASE COUNT         1488 a         2763 c         2668 g         1415 t
ORIGIN      
        1 cgcctcgctc gacgaccgcc atgtactggg acaggtgctc gagctgatgc ccgagaactg
       61 gtccggtcgt ttagaggtcg aggcgatcgt agacggagac gtcaccaacg acggcggggt
      121 acgccacctg accgatcacc agacccggcg ggtagacgaa gggctgttgc tcacgactga
      181 gactgtttcg aagcgaatcc ggttgagttt cgctaccggc gcgaccttgc gggagagaga
      241 cggcggcgag ttgccctccg aagacgcgtc gacctcgacc gctctggttc agcggtggag
      301 cttcgacgcg gcgcagggcc ggagctacac catgcgcaag atcgtcacgg tgttcacctc
      361 gcgcgacgat ccggaccccg acgctcgggc cgttgagaag ctggaggagg cggttgggac
      421 cggcatgccc accttgctcg agaggagcgc gcaggcctgg gcagcgcgct gggactccgc
      481 ggacgtggag atctacggcg acgatgacac ccagcgcaag gcgcgcttcg ccatctacca
      541 cctgatcggc tgcgccaacc ccgaggatga gtacgcgtcg gtgggggcgc gctcgcttac
      601 cggcgagcgc tacaagggcc acgttttctg ggacacggag atattcgtgt tgccgttctt
      661 cgtctacacg catccgccga ccgctcgggc gctcttgatg taccgctacc acacgctgcc
      721 cgctgcccgt gagagggccc gcgcgatggg ataccggggc gctctctacg cctgggagtc
      781 caccgacacc ggggcggacg tcacgcctcc gttcgtctac aactcggccg gcgagcgcct
      841 ggagatcctg accggcttgc aagagcacca catctcggcc gacgtcgcct acgcgatctg
      901 gcagtactgg cgcgtcacgc gggacgaggc gttcttctcg aacgctggcg ccgagatgat
      961 gctggagata gcgcgcttct gggcctcgcg ggccgagcgc ggcgaggacg ggcgctacca
     1021 catcctgaag gtgatcgggc cggacgagtt ccacgagcac gccgacgaca acgccttcac
     1081 caacctcatg gcgcagtggg cgctgcgtcg cggcctggag accgtcgagt ggttgcgcaa
     1141 gcaccacccg gaccgctggc gggagctggc gggccggatc ggtctcgacg agtccgagct
     1201 cgcgttgtgg gaagaagtgg cggtagggct ggtcgataac ttcgatcccg agaccaacct
     1261 cttcgagcag cacagtggct acttcgagct ggaggacatc gacctgcgag acttcgagcc
     1321 gcgcacgaaa tcgttagacg cgatattggg ctggtccagg ctcacacggt cacagatcat
     1381 caagcaggcc gacgtgataa tgctgctctt cctgctcggt gataagtacc cgcgcgaggt
     1441 acacgaggcc aacttccgct tctacgagcc gcggaccaca cacgacagct cgctgagccc
     1501 tagcttccac gcgctggcgg ccgcgcgcgc cggcgatctc gagacagcca gacgctactt
     1561 cgagcgggcc gccaacctcg acctggactt cgcccacggc gtaacggcgg cgggcggcgt
     1621 gcacatcgcg gctctcggcg gcatgtggca cgccatggtc ttcggcttcg ggggcatgta
     1681 cgtcgaagac gaaggccccc ggttcgaacc gcacgttccg gaagactggc agacgctgca
     1741 cttctcggtg ctgtggcgcg gattgcaact gtgggtctcc acaaccggca acgtcgccga
     1801 agtgcaaaag gtcgaaaaag agccgactcg cgtacgcccc ggcccgccag agtaagctcc
     1861 cctaaagcac gccgcttcgc ggcttgtcag ctaggatggc gcacttcgtc gctccgcggc
     1921 gctagacata gcctctgcca caacgccgtc acgtaatgcg tgaccgggcg agatccaaac
     1981 ggcagagagt aagctgaaat gctgagaagc gagggccttt agaaaaggcc cgagcgtgct
     2041 gagaagccga ccgaagggag gcgtgctgaa attcgcttca ggcctcgctc aataattcca
     2101 gaccttttcc ggtgaacctg tagaagtcaa tcccaggcac ggtgccgacg cgctcctccc
     2161 acacaagaac cccctctcgc accaggtacc tgagcgccct ctcgtaacgc agggtgtcag
     2221 gatccagccc cgcccgcccg gccgccaggt acggcgctag gcgcatcccc gcggagaacg
     2281 gcgcacccgg gtcgtgacgc tcgtggtatt cctgcatcgc ccgtagcagg gctacggcgt
     2341 cggtctttac ctcgtagaac gtggcaaccc ctcgatcctg ttcccgcttt gcgttatcgg
     2401 ccgccggcgg gacgcccttg agaaaagcga gtgtggccgc gaccccagcg tcgctccggc
     2461 agtacttaac ttcgcacatc atccaggctg cgacaacgtg aacttgcctc gaccagacgc
     2521 tacccttgct tcagcttggc cgtgtaaagc ttctggttca gggttagcct ggtacgccga
     2581 atctttgggt tttcgccgct gacgggcgag tttgcgggtt actccccttt tacgggcact
     2641 tctacgccat cgaaggttct gtcccgggcg ctttcggtcc cgacccgcaa ggttcccttt
     2701 ccgccaaagg gcggaagatc cagggtcgcc tcgaagtatc cccaggtgga ggtccagtcg
     2761 ttgctcagga cggtacgctg gatcacggct ctaccactgg agtcctccag ggcgatggtg
     2821 ttcgacgcct cgaagttgcg cgagtacccg cgcacggtga gtgggtcccc gatctgtgcg
     2881 cccgagcgcg gctccacgag caccgtgtcg ccgcctacca caggcgggcg cacgtccgag
     2941 ccctcggatc tgaagtccac cacgagacgc gccgggtcgg atagctcgag caccctgtag
     3001 gcgaacgact tgcgcgcgaa gatgtcgacg aacatgccgc cctccggcgc gcgcacgacg
     3061 tagaagctct ggagcagggc gtcgcccagg gtgccgtcgg agacggcggt acggctcatc
     3121 gacggcagcg ttacgcgcag gagaccgctg ccggtcgagc tcatgagggt ccactccggc
     3181 accgacgcgg cgggctcgtc gccggtaccg aggtccagga ccacccgctc gtacccctga
     3241 tgtgccccgt accggaccgc gagcaccgtg tcagcctcgt agctagagcc cccgctcgcc
     3301 tcctgccgcg tgatgaacgg gacgccggag gaatcgcctt tctcccgggc gtacgcggcc
     3361 acccccaccg tcgcttcggg cgcggcgtcc gcagtgcggg gccctccgcc tcccgcttct
     3421 ggggaggcgg cgacggaccg atccaccgaa tcggggctcg agcaggcggc gagcgccccc
     3481 aggagcaaca agatcggcag gaggagagcg catcttctgg cgtcggatct gtgcggtccg
     3541 aacatcgctc agattatagg gatgccgtgt tgcgccccga ttaataggat gtttcgctcc
     3601 tgttacaatg gctgtgtttt tccgttcgcg cccacatgct ctcgaccggc gacaaagcca
     3661 ggcgagagag ctctgttcac taccgcacga acggaggtgg ctgatgatca cgccggatga
     3721 cctcaggctc gcgcagcgcc gggttcgcgg cgtcgcgctc cgcacgccgc tggtaccgtg
     3781 tccgcgcggc gaggagagcc gcctcctcta cttcaaggcg gagaacctgc aaccgaccgg
     3841 cgcgttcaag ctgcgtggcg cctacaacaa gatctcctcc ctctcccccg aggagcggcg
     3901 gcgcggggtc gtggcccatt cgagcggcaa ccacgcccag gcagtggcct acgcggccag
     3961 cgctctcggc gtgcgggcct taatcgtcat gccgcggggc gcgccgcgcg tcaagaccga
     4021 cgccacggct gccttcggag ccgagatcct gttcgtcggc gacgacagcg ccgagcgggt
     4081 gcggagagcc gaggagctgg ccgccgagca cggctacgtc ctcgtgccac cctacgacga
     4141 cgagaccctg atcgccggcc agggcacggt ggggctggag atcctcgatg acctgcccga
     4201 cgtggagacc gtgctggtac ccgtcagcgg tggtggtctt ataagcgggg tcgcggcggc
     4261 gatcaagcag agccgtcctg aagcgcgcgt gttcggggtc gagcccgaac tcgctgccga
     4321 cgcccaggcg agcctgagga gtgggaagct cgtcgagctc cccgcagagc aggtagggcg
     4381 cacgatcgcc gacgggctgc gcgtgcgcaa gctcgggcac gcccccttcg agcacatccg
     4441 tgccttcgtc gacgacatca tcaccgtgag cgaggacgag atactggagg cgatgcgccg
     4501 aatggcgcta cggacgcggc tcgtagccga gccgagcggg gccgtcacct tcgccggcta
     4561 cctcttccac cgcgacgagc tccccgccac acggctgaac gtcgccgtcg tgagcggcgg
     4621 taacgtcgag ccacaactcc tcgccaggat tctcgctgat tagcacgcct cccttcggtc
     4681 ggcttgtcag cttctctcta cactgactgg ctgagaagcg agggcccctg ggcccgagcg
     4741 tgctgaaatg ctatgttgcc agatacggcg tggtgccgta ttgctccatg cgaagaaccc
     4801 cgccctcctc gccgagctcc atctgcaggc gatcactgcg tcccctgaag ccgaactcgc
     4861 gaaacagctc gtgcgcggga ctctccacga tgccgggcac ggtgacctcc accggcgatc
     4921 caacgcccgc gcgcagagcg gcggccagga ggagccgggc cacgtccggg atcgaggcga
     4981 cgaagggtcc gatgcggtcc acgcgccccg aatagcttcg gagcaggtag cctttgatcc
     5041 ttcccgaaga gtcgcgggct atgagacccc cacccggatg cagcctcagg gtcgcgaaga
     5101 tcagagccga cctgtctgcc ccgtaggacc agtgatccag gccgtagagc tccggcagat
     5161 cgccgaacga gagcgtccca accctgtgcc cgtctacttc ctctccccca ccggcgcgcg
     5221 tcgccttatc gaggcggtac acggtgcgcg gcgcgcgcgg cctgaagccc agggagcggt
     5281 acagcccctc ggcctgccgg gtcgagtaga gtcgtattac ctccgctccc tgcgaccgga
     5341 ggtatgcgac ggcccaggcg gagagacgca cgccgaggcc catgttgcgg tggtcgtccc
     5401 ggacggcgag gtggcacagg atcccgatcc tcccgagcgg caccgcgccg accatcccgg
     5461 ccagggagcc gtccgaagcg tccgccacag tccaccgggc ctcgcgcatc ccgcgcaaga
     5521 gatcgaacgc ccgcggatcg gcggcccagc tcaccaccga ccggagttcc cggacgcgct
     5581 cgagatccac atcgtccatg ggccgcatgc gaacgccgga gagtccttcc gcaccgggta
     5641 gcatccacag cccgccacga cccgcggcct cgttccgttc ggccattcct tctgcatttg
     5701 tgaaagccag caagatatcc cccgatgatt tatgtggata gagagagctc tccacgatga
     5761 gtgaggtgtg cggaagcccc gagggcgccc tctacaagga cggtcggggc agggacggtc
     5821 gaggaagccg ccccgagaag gctcctggaa taagccctat gtagcccgca tccacgcacg
     5881 acatgctaca acatggcgcg ccgcggggaa agtgcgtgct cgaagatcca gcaccatcgc
     5941 ggccgctgta atttggcttg ctaactgatt tgtgggcggc taagagcatt tttattgcaa
     6001 gtgcggacca ttttgttaca atgctctcac aactgactga actagcgaca aggagcggcg
     6061 aaaggtgcga gccacgctac agcccggcct gacgaacagg ctgacgaagt atcttcggac
     6121 gacccagcag cttatcgagg agggccgcga cgcggtctcg agcaaggagc ttggagactt
     6181 caccggcata aatccggttc aggttcggag ggacctgaac gccatcgggt tctccggcac
     6241 ccgcggggtg ggctaccagg cctacgatct cgtcgaggct gtgaggaaca tcctggggtt
     6301 gaggcagacc tacaacatcg cgctggttgg cgcgggcaac ctcgggagcg ccatcgcggc
     6361 gagcactatt ttgcccaaga ggggcttcgt cattcacgac gtcttcgaca acgacccgga
     6421 gaagatcgga cgcaccgtcg gcaacgtcgt cgtcaagcac atagacgagc tcgagaagag
     6481 cgtagccgag gccgaggaga tcatcggcat catcgccacc cccgcctcct ccgcccagga
     6541 ggtcgccgac ctgatggtag acgccaacat ccgcgtcatc ctcaactaca ccgatgtcct
     6601 cctccacgtc ccgtcccacg tcgacgtcca ccgcatagac ccgaccgccc agctcatgca
     6661 caccctttat tacctgaccc aggcagaagg acaggaagct tcgtaacgca gcacgctcgg
     6721 gcctttagta aaggccctcg cttctcagca tttcagctag tcagtgctga gcgaagctga
     6781 gaagccgcct ggcgaagccc aaggcggcgt gctgacaagc gaaagccgaa tgctgcagcg
     6841 tgctgaagag aagggaggcg tgctgacctt acgggtcccg gttctcgccg cgctcgagcc
     6901 cttcgaggat ggggccgagc gcccgttcgc gggtggcctc gtactcttcc aaagagcttg
     6961 cttccctggc gagcctggcg acgaggcccg tcagtcccgt accgcctttg gccgcgagta
     7021 tgcgccaggc gaggagacgg tatgagcgct cgagccgcgg gtcattgcgt ccaccctcga
     7081 gcaactcctc cagcgccgcg cacagagctt ccgctgcttc cagaggaagc tctaccgaag
     7141 gaccgccggg tgagctaacg ggttttctcc acgtagatgc ggtcctgggt gatgattaca
     7201 tgcaggtcgt ctggggagcc ccgcatgagg cccctccact cgcggtcgaa gacctcggag
     7261 ttgcgagcgt cggtcctgag caggatgcct tcttctacct cgtcaaggac tgcggggtgc
     7321 tctgaaagct ggccgcgccg gaccgggtcc cggcggtaga tcgagttccg cgaccggaag
     7381 ctctgcacgc tcgagggagt tgtgttgagt tcctgcgcga tccagtcgtc gttgcgcccc
     7441 tcctcgaccc aacgccggat cttcgatgcg tgtggcttta gcgccaccct tctatttccc
     7501 acgacccgtg ctccttatac gaactagttt tatagtccat atgtaccact ttttgttacc
     7561 cggcgctaga tgaattttat gtttatatat gaattttatg tttagccttg gaaaggagct
     7621 tgttcagtcc accgcggtcg agagatggtc tgcggcggcg aggccggaga gagcggcacc
     7681 ctcgaccttg gagggaccga aggcgtcccc ggcgaatagg agcggaggat ctccgctcgc
     7741 cacgaagcag ggctccggat gtggctgcgt gacccagctg tagcgccagc ggacgagaga
     7801 cgtctcgagc accctggacg ggaggtcggt cgccagttgt tctgcggcga aggctagtag
     7861 agaggcggcg accagtgcct cctcggactc gaagttctcg cggctccact ccggtccggc
     7921 gtggatggtt atggctggcg cctccgatat gccctttctc tggttgtccc cgatccagtc
     7981 cagcggctcg cctttgatct ggataccgcc gggctcgggc acgccggtcg gcccattcat
     8041 cagcgccatg agcgcgaggc agggggagta ggtaatcccc gcgagccgct gccgcgtctc
     8101 ctctgacaac gcggagccgc cctcgaagag caagagcgat tgcgggaccg gcgccgtgag
     8161 cagcaaagct gtccccgcta cctgtgatcc cgcctctgtg cgcgcttccc aaactttgtc
     8221 ccgaatcttt acggccacga cccgctcgcc ggttcttacg tcaaggcccc gggcgaggtg
     8281 atcggctacc gaggacatgc ccctggagcc acggtaccgg ggatagccgt cttc
//
LOCUS       DASTUV010000451         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1363853, whole genome shotgun sequence.
ACCESSION   DASTUV010000451 DASTUV010000000
VERSION     DASTUV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363853"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /locus_tag="VFT03_09200"
     CDS             <1..1800
                     /locus_tag="VFT03_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4848363.1"
                     /translation="ETRFRGHHLLHEHGVVSGMTVIIRPGGRPFGVLGAHTRERRTFT
                     GDDVNFLKAVANVLAAAIERERADEKQRFLADTGALLSSSLDYRATLGSVARLAVPTL
                     ADWCAVDILDHGSIERLAVEHPDPEKVVLAMKLQERYPQDPESLGGIYGVLRTGRPEF
                     YPEVTDEMLVAAARDEEHLALLRELGFTSAIVVPMIARGKTLGAISLVSAESGRKYDE
                     ADLDLANELALRAALAVDNARLYEEAQTEIAEREWAQTELRSSRDELRVILESVADGV
                     TAQDPTGRIIYANEAAARMIGYSSGQALVEASQRELMERFEVFDEEGRPFPLEKLPGR
                     RALAGEEGTDEVLCFRELATGEERWSIVRAAPVLDDRGRVRMAVNIFRDITERRRTEE
                     TLREIRDSERRRIARDLHDGVLQDLSYTAAAMGLIMLDVEGTDLEGELRSTTDAVRRA
                     AQGLRDAVNDLRLEEERDRPFPELVKSLVRRNRARTPDCEITLEVEDGFPSTPLGETG
                     AQLARVVQEAITNARRHSGARKVSVTLKTEGEDLVAEVSDDGRGIGPDTTPGVGLSSM
                     SERAAIIEGTLTIDSEAGRGTRVNLRAPDPQKG"
     gene            1803..2210
                     /locus_tag="VFT03_09205"
     CDS             1803..2210
                     /locus_tag="VFT03_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848364.1"
                     /translation="MQGGDVATRVRVLLVEDQADFRRLMVALLARQPDLEVVAQAGSL
                     TEARRRAETVVFDVAVLDLGLPDGNGVDLISDLRWSNPDAAVLILSASLDPASLERAA
                     DAGANEIMDKLATPIELIGAIRRLGTGESTDNL"
BASE COUNT          423 a          670 c          821 g          364 t
ORIGIN      
        1 gagacgcgct tcagggggca tcatctgttg cacgagcacg gagtagtgag cggcatgact
       61 gtgatcatca ggcctggagg gcgaccgttc ggcgtccttg gcgcgcatac cagggagcgc
      121 aggaccttta ccggggacga cgtgaatttt ctcaaggcgg tggccaacgt gcttgccgcg
      181 gcgatcgagc gcgagcgggc cgacgagaag cagcgcttcc tggccgacac tggcgcgctg
      241 ctctcttcct cgctggacta cagggcgacg ctggggagcg tggcccggct cgcggtgccc
      301 acgctggcgg actggtgcgc ggtggacata ctggaccacg gctccatcga aaggctcgcg
      361 gtggaacacc ccgacccaga gaaggtcgtt cttgccatga agttgcagga gcgttacccg
      421 caagatccag aatccctcgg cgggatatac ggcgtgctgc gaacgggccg tcccgagttc
      481 tatcccgagg tcacagacga gatgctcgtg gccgcagccc gcgacgagga gcatctcgcg
      541 ctcctgcgtg agctcggctt cacgtccgcg atcgtcgtgc cgatgatcgc gcgtgggaag
      601 acgctgggag ccatcagcct ggtctcggcc gagtctggtc gcaagtacga tgaggcggac
      661 ttggatctcg cgaatgagct tgccctcagg gcggcgctgg cggtggacaa cgccaggcta
      721 tatgaggagg ctcagacgga gatcgccgag cgtgagtggg cccagacgga gttgcgtagc
      781 tcgcgcgacg aactccgggt catcctcgag agtgtcgccg acggcgtgac cgcgcaagac
      841 cccacggggc ggatcatcta cgccaacgag gctgcggcca ggatgatcgg ctactcctcg
      901 ggccaggctc tggtggaggc gtcgcagcga gagttgatgg aaagattcga ggtcttcgac
      961 gaggagggca ggccgtttcc gctcgagaag cttccggggc gcagggcgct cgcaggcgag
     1021 gaaggaaccg atgaggtctt atgtttccgc gagcttgcga cgggtgagga gcggtggtcc
     1081 atcgtcaggg cggcgccggt cctcgacgac cgggggaggg tccgcatggc ggtcaacatc
     1141 tttcgcgaca taaccgagcg ccggcgcaca gaagagaccc tgcgggagat cagagactcc
     1201 gagcgcaggc ggatagcgcg tgacctgcac gacggcgtcc tgcaggacct ctcctacaca
     1261 gcggcggcca tggggctcat catgctcgat gtcgaaggca cagatctgga aggcgagctg
     1321 cggagcacta ccgacgcggt ccgtcgcgcc gctcaggggc tgcgcgacgc ggtcaacgac
     1381 ctgcgcctcg aggaggagcg agaccgcccg tttcccgagc tggtgaagtc cctggtgcgc
     1441 aggaaccggg cgaggactcc agactgcgag ataaccctgg aagtggaaga cgggttcccc
     1501 tcgacgccgt tgggggagac cggagcgcag ctggcgcgcg tagtccagga ggctatcacc
     1561 aacgcgcgcc gtcactccgg tgccaggaag gtctcggtga ccctgaagac agagggggaa
     1621 gacctggtcg ccgaggtctc tgatgacggg cgggggatcg ggccggacac cacgcctggc
     1681 gtgggcttga gcagcatgag cgagcgggcg gccatcatcg agggtacact aaccatcgat
     1741 agcgaggctg gacggggaac cagggtgaac ttacgggccc cggacccaca gaaaggataa
     1801 gtatgcaggg gggagatgtc gcaacacggg tccgcgtgtt gctcgtcgag gatcaggccg
     1861 acttcaggcg cctgatggta gccctgctgg cccggcagcc ggatctcgag gtggttgctc
     1921 aggccgggtc tcttaccgaa gcccgcagac gcgcggaaac ggttgtgttc gacgtggcgg
     1981 ttctggatct tggtcttccc gacgggaacg gtgtggactt gatctcggat ctgcgctggt
     2041 ccaaccccga tgcggccgtg ctgatcttga gcgcaagcct cgacccagcg agcctcgaga
     2101 gggcagccga tgctggcgcg aacgagatta tggacaaact agctaccccc atcgagctta
     2161 tcggcgcgat aaggcgcctc gggacagggg aatccaccga caacctctga tgtcgctgtg
     2221 ggcgcggccg gatcagcccg tccatgcttg aggtagcgag gcatccggag aaccgcta
//
LOCUS       DASTUV010000452         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1676644, whole genome shotgun sequence.
ACCESSION   DASTUV010000452 DASTUV010000000
VERSION     DASTUV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676644"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFT03_09210"
     CDS             complement(<1..320)
                     /locus_tag="VFT03_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848365.1"
                     /translation="MLETILHPLVEFVTRTIGAYGVPAVFVLMVLESMGILIPSEAIS
                     PFAGYLVSEGRMTFFAAVAAGVLGNLIGSWIAYCIGLWGGRELWFHYGRYVGVRVHHL
                     HLAE"
     gene            complement(375..1100)
                     /locus_tag="VFT03_09215"
     CDS             complement(375..1100)
                     /locus_tag="VFT03_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4848366.1"
                     /translation="MLKTLLETRAEMFRKIADRLTLAAAIWLAAGLAFSAFVVWAFAE
                     LADEVVEGDSRRFDRAVLLWIHSNFPGWLDGPMRLVTALGYYWVVIPLLAVAVLAFYR
                     KGWKLSAILLVVSTSGSLVLMTVLKAIFERSRPDFFDSGYTASFYSFPSGHATLAVGF
                     YGTLTLLLAYRLRGYARWLVAACGVSLVLLIGFSRLYLGVHYPTDVLAGYLAALLWLV
                     FVGVAYAFWLSVRGLRAAETRRE"
     gene            1207..2166
                     /locus_tag="VFT03_09220"
     CDS             1207..2166
                     /locus_tag="VFT03_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848367.1"
                     /translation="MIRLYAAADSAVTIVENGRVERTLEGRGARCVAVDPNDPDTLYV
                     GTTDEGLFKSTDGGRSWEPLPGVEHPRVTAVAVSPADGALYAGTEPSALFVSRDGGAS
                     WRELEGLRNLPSAPTWSFPPRPWTSHVRAISLSYTDPNLVVAGIELGGILRSPDGGET
                     WQDQRPGAYADCHSLAAHPLSSEALYEAGGGGFAESGNFGDSWRAADDGMGLHYVWGL
                     AVDSEDPSLIYTSAAPGPYQAHGSGFSDAAIYRRRDGGRWEPLLEGLREFPYALAADP
                     GTSGALFAGFGDGEILRSPDAGATWDEVPRTPGGLAALAVVQI"
     gene            complement(2186..>2585)
                     /locus_tag="VFT03_09225"
     CDS             complement(2186..>2585)
                     /locus_tag="VFT03_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU4848368.1"
                     /translation="ATEAVAHLAERWPLAVASSSNRPIIDLVLELSGLDRFFRATVSS
                     EEVSRGKPAPDVYLEAARRLGADPERSAAVEDSSSGILSARAAGMRVVAIPNMRFPPG
                     EDALAAADVVIPSIGELTPDVVEGSELPRR"
BASE COUNT          475 a          868 c          885 g          357 t
ORIGIN      
        1 ttctctgcta ggtggaggtg gtgcacgcgc accccgacgt agcgcccgta gtggaaccac
       61 agctcgcgcc cgccccacag cccgatacag tacgcgatcc aggagccgat caggttcccg
      121 aggaccccgg cggccaccgc cgcgaaaaac gtcatcctgc cttcggagac caggtagccg
      181 gcgaagggtg agatcgcctc ggaggggatc aggatcccca tgctctcgag aaccatgagc
      241 acgaataccg cgggcacgcc gtaggctccg atggtcctcg tcacgaactc cacgagcgga
      301 tgcaggatgg tctcgagcac gcggtgaagc taacacctcc cagaggtgtt ttccactatc
      361 cccggactcc gacgctactc gcgccgcgtc tccgcggccc tcaggccacg gaccgatagc
      421 cagaacgcgt acgcgacccc gacgaagacc agccacaaga gggcggcgag gtacccggcc
      481 agcacgtccg tcgggtagtg caccccgagg tagagcctgc tgaaaccgat cagcagcacg
      541 agcgagacgc cgcaggccgc cacgagccac cgcgcatagc cgcgcaaccg gtaggcgagg
      601 agaagggtga gcgtcccgta gaacccgacc gctaacgtag catggccgct cggaaacgag
      661 tagaacgagg cggtgtaccc cgagtcgaag aagtcgggac gcgaccgctc gaagatcgcc
      721 ttgagtacgg tcatcagcac caggctcccc gaggtcgaaa cgacaagcag gatggcggag
      781 agcttccagc ccttgcggta aaaggcgagg accgcaacag cgaggagcgg gatcacgacc
      841 cagtagtagc ccagagcagt gacgaggcgc atcgggccgt cgagccaccc ggggaagttg
      901 gagtggatcc agagcagcac ggcccggtcg aaccggcggc tatcaccctc gaccacctcg
      961 tccgccagct ccgcgaatgc ccaaaccacg aacgccgaga acgcgagccc ggcggcaagc
     1021 cagatggctg ccgcgagcgt cagacggtcc gctatcttcc gaaacatctc cgcgcgagtc
     1081 tcgagcagcg tcttgagcat gggaagcatt ctatccgctc gcgctcgaca gccgcttgtt
     1141 ctggaaacat cactgccgca tactcgtaac tgtagagaga agcggtatcg agagaggtga
     1201 ccaggtatga tcaggctgta cgcggccgcg gacagcgccg tgactatagt ggagaacggt
     1261 agggtcgagc ggacgctgga gggacgcggc gcgcggtgcg tggcggtaga cccaaacgat
     1321 cccgacaccc tctacgtcgg caccacggac gagggtctgt tcaagagtac cgacggtgga
     1381 aggagctggg agcctctccc tggcgtcgag cacccgaggg tcacggccgt ggcggtctcc
     1441 cccgctgacg gggcgcttta cgccggcacg gagccgagcg ctctgttcgt cagccgcgac
     1501 ggcggagctt cctggaggga gctggagggc ttgaggaacc tcccatccgc gccaacgtgg
     1561 agcttcccgc cccgcccctg gacctcgcac gtgcgcgcga tctcgctctc ctatacagac
     1621 cccaacctcg tggtggccgg catagagctc ggcgggatcc tgaggagccc ggacggaggg
     1681 gagacctggc aggaccagcg gcccggcgcc tacgccgact gccactctct ggccgcgcac
     1741 cccttatcct ccgaggcgct ctacgaagcg ggcggcggcg ggttcgccga gagcgggaac
     1801 ttcggtgata gctggcgcgc agccgacgac gggatggggc tccactatgt ctggggactg
     1861 gccgtggact cggaggaccc ctctctcatc tacacttcgg ctgcccctgg cccctatcag
     1921 gcgcacggga gcggcttctc ggacgcggcg atctaccgcc gcagggacgg tggacggtgg
     1981 gagcctctcc tggagggcct gagggagttc ccctacgccc tcgccgccga cccggggacg
     2041 tccggcgccc tgttcgccgg cttcggggac ggcgagatcc tgcgcagccc cgacgcgggg
     2101 gcgacctggg atgaagtccc ccgcacgcca ggcggactgg cggcgctcgc cgtcgtacag
     2161 atctaggggt ccgaaccgca ccgcctcacc ggcgtgggag ctcgctccct tccacgacgt
     2221 ccggcgtgag ctcgccgatg gaggggatga cgacgtccgc cgcggcgagt gcatcctcgc
     2281 cgggcgggaa gcgcatgttg gggatcgcca cgacgcgcat acccgctgcc ctggccgaga
     2341 ggatgccgct actggagtcc tccacggcgg cgctcctctc cgggtcggcg ccgaggcgcc
     2401 gtgcggcctc gaggtagacg tcgggggcgg gcttgccgcg cgacacctcc tcggaggaga
     2461 ccgtcgccct gaagaaccgg tcaagtcctg agagttccag gaccaggtcg attatggggc
     2521 ggttcgacga ggaggcgacc gccagcggcc agcgttcggc gaggtgggcg acagcttcgg
     2581 tagct
//
LOCUS       DASTUV010000453         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1048854, whole genome shotgun sequence.
ACCESSION   DASTUV010000453 DASTUV010000000
VERSION     DASTUV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048854"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFT03_09230"
     CDS             <1..595
                     /locus_tag="VFT03_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU4848369.1"
                     /translation="RLGDLATRITAGRKRAAQELSAKVQGNLADLNLEKTTFTAELVP
                     TSPGPSGVERVEFVIQPNPGEPELQVRHYASGGELSRIMLAIRLAQERIEPGATYIFD
                     EVDAGIGGETATAVGAKLRELGERNQVLTITHLPQIASEALSHIVVTKEEAGGRTITR
                     IRRVEDEERLKELARMLSGRIDEASLAHASELLVGGR"
     gene            592..1764
                     /gene="steA"
                     /locus_tag="VFT03_09235"
     CDS             592..1764
                     /gene="steA"
                     /locus_tag="VFT03_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEU4848370.1"
                     /translation="MRPREGFGLNGSRGEFYLTGKAILGRKTKEILPLLDGVTIPVID
                     HENLDRITAETLVEAGVNAVVNAAGSTTGKYPNMGPFILARAGVYILDGVGRKVFERL
                     STGDEIELRGDGVYKEGELLAVGEHLDEETAERRLRESRRVVGVALESFAQNTIEFMR
                     HEQDLLFSRLKVPTDLARAMRGRQVLVVVRGYDYRQDLAALRTYLREVRPFIIGVDGG
                     ADALLEAGWKPNLVFGDMDSVSEEGLRLADRLLVHAYPDGNCPGLERLRAAGIADAET
                     LPAPGLSEDVAILIAEQCGAELIVAVGTHVGLVEFLDKGRRGASSTFLTRLKVGARLV
                     DAKGVSKLYPSRVSVWQLVALVVTALVAISAIVYSSHSLADIFQILAIKLRLLLGI"
     gene            1768..2577
                     /locus_tag="VFT03_09240"
     CDS             1768..2577
                     /locus_tag="VFT03_09240"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEU4848371.1"
                     /translation="MPDLRYHLISLISVFLALAIGILLGVAMADRGVVSNRVQAEITG
                     IQQQLDRQQREIARRDEQIADRDALLEGMSEVMISDSLKGKDVALVAGPFADSEVSDA
                     VQSALSEAGANITTFETLELPSPDEITGQELTTQEATTQLSSGYADDFASQVLGFDSE
                     EAEVPDIIIFVGGGEIPPDAQPGTLQALKSVQRRIFDIWLDSGTRVIGAEPTDAGRTE
                     VPFFQNAGVPSIVNADQPAGRAAIIRCAKMEDCEGTYGTKESAAEAFPPPS"
     gene            2601..>3052
                     /locus_tag="VFT03_09245"
     CDS             2601..>3052
                     /locus_tag="VFT03_09245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4848372.1"
                     /translation="MIPARDEAATLADTLRALHTIPNLSHLIVADDGSRDTTPDIALA
                     LGAELVHASPPGKPAGKGHALRHGLIRVRPHPPDAVLLLDADLGYSAALLATLIAALD
                     EDIPVSIAAFPPATTKGGFGLVKGFARSAIASRTGYLSSEPLSGQRALL"
BASE COUNT          546 a         1067 c          968 g          471 t
ORIGIN      
        1 gcggctcggg gatctcgcga cgaggataac ggcgggcagg aagcgggctg cgcaagaact
       61 ctcggcgaag gtgcagggta acctggctga cctcaacctg gaaaagacga ccttcaccgc
      121 cgagctcgta cccacgtctc ctggtccttc cggcgtggag agagtggagt tcgtgatcca
      181 acccaacccc ggcgagccgg aactgcaggt aaggcactac gcctctggcg gcgaactgtc
      241 gaggatcatg ctcgccatca ggctcgcgca ggagcgcatc gagcccgggg cgacctacat
      301 cttcgacgag gtggacgcgg ggataggcgg cgagacggcc acggcggtcg gggcgaagct
      361 gcgggagctc ggcgaacgca accaggtcct caccatcacg cacctgcccc agatcgcctc
      421 cgaagccctc tcccacatcg tcgtcaccaa agaggaggcg gggggccgca ccataacccg
      481 catccggcgg gtggaggacg aggagcgcct caaagagctg gccaggatgc tctcgggccg
      541 catcgatgag gcctccctcg cccacgcctc ggagctcctg gtgggcggac gatgagacca
      601 agggaaggtt tcggcctcaa cggctcgcga ggggagttct acctcacggg caaagcgatc
      661 ctcgggagga agaccaagga gatcctgcct cttctcgatg gcgttaccat tcccgtgata
      721 gatcatgaga acctcgaccg catcacggcg gagacgctgg tggaggccgg ggtgaacgcc
      781 gtggtgaacg ctgccggctc cactacgggc aagtacccga acatggggcc gttcatcctg
      841 gcgcgggccg gcgtctacat cctggacggg gtcggcagga aggtcttcga gcggctctcg
      901 acgggggatg agatcgagct tcgcggtgac ggcgtctaca aggaggggga gctcctagcc
      961 gttggagagc atctcgatga ggaaaccgcc gagcgcaggc tccgcgagag ccgccgagta
     1021 gtcggcgtgg cgctcgagag cttcgcccag aacacgatcg agttcatgcg ccacgagcag
     1081 gacctcctct tctccaggct caaggtcccc acggacctgg cgcgtgcgat gcggggtcgc
     1141 caggtgctcg tagtggtgcg cggctacgac tacaggcagg acctcgcggc cctgaggacg
     1201 tacctgcggg aggtaaggcc gttcatcatc ggggttgatg gcggggccga cgccctgctc
     1261 gaggccgggt ggaagccgaa cctggtcttc ggcgacatgg actccgtctc cgaagagggt
     1321 ctcaggctgg ccgataggct cctggtccac gcttaccctg acgggaattg ccccggcctc
     1381 gagcgcctga gggcagcggg aatagcagac gccgagacgc tccccgcgcc cgggctctcc
     1441 gaggacgtcg ccatcctcat cgccgagcag tgcggtgccg agttgatcgt ggccgtcggt
     1501 acccacgtcg gcctcgtgga gttcctggac aaaggccgca gaggggcctc atcgaccttc
     1561 cttacccgcc tgaaggtcgg ggcgcgcctg gttgacgcga agggcgtctc gaagctctat
     1621 ccctcccgcg tctccgtttg gcagctcgtc gcgctcgtcg tcaccgcgct ggtcgccatc
     1681 tcggcgatcg tgtactcctc ccactcgctg gccgatatct tccagatact cgccataaag
     1741 ctccggcttc tcctgggcat ctaggaagtg ccagacctca ggtaccacct gatctccctg
     1801 atctctgtct tcctggcgct ggcgatcggg atcctgctcg gcgtggctat ggcggacagg
     1861 ggcgtcgtaa gcaaccgggt ccaggcggag atcacgggca tccagcagca gctcgaccgc
     1921 cagcagcgcg agatagcccg tcgcgacgag cagatagccg acagggatgc gctgctggaa
     1981 ggcatgtcgg aggtgatgat atccgacagc ctcaagggca aggacgtcgc cctggtcgcc
     2041 ggccccttcg ccgactccga ggtgtctgac gccgtgcaga gcgctctctc cgaggccggc
     2101 gcgaacatca cgaccttcga gaccctcgag ctcccgagcc cggacgagat caccggccag
     2161 gagctcacca cgcaggaagc gaccacgcaa ctttcctcgg gctacgccga cgacttcgcc
     2221 agccaggtcc taggcttcga cagcgaagag gccgaggtac ccgacataat tatcttcgtc
     2281 ggcggcggcg agatcccgcc tgacgcgcaa ccaggcaccc tgcaggcgtt gaaaagcgtc
     2341 cagaggcgga tattcgatat atggctcgac tcagggaccc gcgtgatcgg cgccgagccc
     2401 actgatgccg gacgcacgga ggtccccttt ttccagaacg ccggcgtccc ttccattgtc
     2461 aacgctgacc aacccgccgg acgcgcggcc atcatcaggt gcgccaaaat ggaggattgc
     2521 gaggggactt acggcaccaa ggagagcgcc gccgaagcct tccctccccc aagctagcca
     2581 tggtgctcga gacagcagcg gtgatcccgg cccgcgatga ggccgcgacc ctcgccgaca
     2641 ccctgcgagc cctgcacacc atccccaacc tctcacacct gatcgtggcc gacgacggca
     2701 gccgcgacac gacacccgac atcgccctgg ccctcggcgc cgagctcgtc cacgcctcgc
     2761 ccccgggcaa gcccgcaggc aagggccacg ccctccgcca cggcctcatc cgcgtccgcc
     2821 cccacccccc cgacgccgtg ctcctccttg acgccgacct cggctactcg gccgctctcc
     2881 tcgccaccct catcgctgcc ctcgacgaag acatccccgt ctccatcgcc gccttccctc
     2941 ccgccaccac gaaaggcggc ttcggcctcg taaagggatt tgccaggagc gccatcgcca
     3001 gccgcaccgg ctacctctcc tcggagccac tctccggcca gcgcgccctg ct
//
LOCUS       DASTUV010000454         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1697411, whole genome shotgun sequence.
ACCESSION   DASTUV010000454 DASTUV010000000
VERSION     DASTUV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697411"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFT03_09250"
     CDS             <1..880
                     /locus_tag="VFT03_09250"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEU4848373.1"
                     /translation="VLPCRNVADTIGSIVDEIYAVNRRAGVEPLVDQILAVDADSADG
                     SAEVAASRGAEVYSENELMSTYGGAHGKGDAMWRSLSVARGDLIMYIDADTKDFRPQL
                     VYGVLGPILHLPEVRFVKAAYRRPFKSYESVEADGGGRVTELAAKPLFNLFYPELTGF
                     VQPLAGEFVADKELFCSIPFLTGYAVETGIMIDVLKRVGLGAMAQVDLGTRQNRHQHL
                     RDLSRMSYAVLRAVARRLRQDGRLDQARDPSLPDQLFQFSDYMHAVATPDGLKLQEYV
                     EELVERPPINEVLTVG"
     gene            886..1176
                     /locus_tag="VFT03_09255"
     CDS             886..1176
                     /locus_tag="VFT03_09255"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848374.1"
                     /translation="MHDLGGSPGGPVDRSQHEVEDWERLADAVTIALDIKGLKTTDEH
                     RRAIESLENYRELGYYERWVAATEKLLVEKGILTREEVDKRSAEIERRWSGA"
     gene            1173..1469
                     /locus_tag="VFT03_09260"
     CDS             1173..1469
                     /locus_tag="VFT03_09260"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEU4848375.1"
                     /translation="MTPFEPGERVRVRSADKPGHVRTPEYLKGKTGRIESVLGEFRSP
                     EDLAYGLSGMPERPLYKVSFRQADLWSDYEGPNSDHLYADVYEQWLEHDEGGSA"
     gene            1466..2071
                     /gene="nthA"
                     /locus_tag="VFT03_09265"
     CDS             1466..2071
                     /gene="nthA"
                     /locus_tag="VFT03_09265"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEU4848376.1"
                     /translation="MSHPHEEVRHESSPYAARIRAIEALLLEKGVLTEPEIQDRISYM
                     EARSPANGAKLVARAWVSPEFRELLLSDTKAAAQELGIDASGPVEFVVVENTPEVHNL
                     IVCTLCSCYPRAILGRPPDWYKSFNYRSRAVREPRAVMGELGFEPPEGVEVAVHDSTA
                     DIRYMVLPMRPAGTEDLSEQELAELVTRDDLIGVSVPRYED"
BASE COUNT          402 a          700 c          725 g          336 t
ORIGIN      
        1 ggtcctgccg tgccgcaacg tcgccgacac tatcggctct atcgtggacg agatctacgc
       61 cgtcaacaga cgcgccgggg tggagccgct cgtggaccag atcctcgccg tcgacgccga
      121 ctcagccgac gggagtgctg aggtggccgc ctcaaggggc gccgaggtgt actcagagaa
      181 cgagctgatg agcacctacg gcggggcgca cgggaagggt gacgcgatgt ggcgctccct
      241 ctcggtcgcg cgcggcgacc tgattatgta catagacgcg gacacgaagg acttcaggcc
      301 ccagctcgtc tacggtgtcc ttggccccat attgcacctc cctgaggtgc gtttcgtcaa
      361 ggccgcctac cgcaggccgt tcaagagcta cgagtccgtc gaggccgacg gcggaggcag
      421 ggtcacggag cttgcggcca agccgctgtt caacctcttc taccccgagc taaccggctt
      481 cgtgcagcct ctggccggcg agttcgtcgc ggacaaggag ctgttctgct ccatcccctt
      541 cctgacaggc tacgcggtgg agaccgggat catgatcgac gtcctcaagc gggtgggcct
      601 cggggcgatg gcgcaggtgg accttggcac ccgccagaac aggcaccagc acctcagaga
      661 cctgagccgt atgtcgtacg cggtgctacg ggcggtcgcc cgtcgcctga ggcaggacgg
      721 ccgtctcgac caggcgaggg acccgagttt gcccgatcag ctcttccagt tctccgacta
      781 catgcacgcg gtggcgaccc ccgatgggtt gaagctgcag gagtacgtcg aggagcttgt
      841 cgagcgaccg ccgatcaacg aggtcctgac ggtcgggtag ccgcggtgca cgacctcggc
      901 ggctcgcccg gcggcccggt agacaggagc cagcacgaag tcgaggactg ggagcgcctc
      961 gccgacgccg tcaccatcgc ccttgacata aaggggctca agaccaccga cgagcaccgc
     1021 cgcgccatcg agagcctgga gaattacagg gagctgggct actacgagcg gtgggtcgcg
     1081 gcgacggaga agctgctcgt cgagaagggc attctcaccc gcgaagaggt ggacaaaagg
     1141 tcggccgaga tcgaacgtcg ctggagcgga gcgtgacgcc gttcgagccg ggcgagcggg
     1201 taagggtgcg ttccgccgac aagccgggac acgtgcgcac cccggagtac ttaaagggga
     1261 aaaccggcag aatcgagtcg gtgctcggcg agttccgcag ccccgaagac ctggcctacg
     1321 gcctctccgg aatgccggag agacccctct acaaggtgag cttccggcag gcggacctct
     1381 ggagcgatta cgagggtccc aactccgatc acctgtacgc agacgtctac gagcagtggc
     1441 tggagcacga tgaaggaggt agcgcgtgag ccacccgcac gaggaggtca ggcacgagag
     1501 cagcccctac gcggcgcgaa tccgggcgat agaggcgctg ctcctggaga agggcgtgct
     1561 aacggagccg gagatacagg accgaatctc ctacatggag gcccgctcgc cagccaacgg
     1621 cgcgaagctc gtggcccggg cctgggtgag ccccgagttc agagaactcc tcctctccga
     1681 cacgaaggcc gcagcacagg agctcggcat agacgcctcc ggcccggtcg agttcgtcgt
     1741 ggtcgagaac accccagagg tgcacaacct gatcgtgtgc accctgtgtt cctgctaccc
     1801 gcgggccatc cttggccgcc cgcccgactg gtacaagagc ttcaactacc gttccagggc
     1861 cgtcagggag ccccgcgccg tcatgggcga gctcgggttc gagccccccg agggcgtcga
     1921 ggtggccgtc cacgacagca cggccgatat ccgctacatg gtgcttccga tgcgcccggc
     1981 aggcacagaa gacttgagcg agcaagagtt agccgagctt gtgacgcggg acgacctgat
     2041 cggggtctcc gttcctcgct atgaagatta gcacgatccg gcctgcggcc tccgctcgga
     2101 gcgtgctaac tagctgaccg ctttctcctc ctccctctcg ttggcggcga ggatcctgtc
     2161 cac
//
LOCUS       DASTUV010000455         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1263575, whole genome shotgun sequence.
ACCESSION   DASTUV010000455 DASTUV010000000
VERSION     DASTUV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263575"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFT03_09270"
     CDS             complement(<1..858)
                     /locus_tag="VFT03_09270"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848377.1"
                     /translation="MLGRYAELKAQLPQGTILFYQVGTFFETFEEDAKTISRELSIRL
                     TSREAAGEGRVPLAGVPGHALQEHVAVLLRKGHSVAIAEQRPHPTRPKQFTREISRVL
                     TPGTVIEDNVLSAGHANYLAAINVHDGMVGVAIVEASTGEFTGTEVPEEDLAAELERW
                     SPREMVVPERTAAEDLPKVRAAISSAPRWTFEPSAGERALTRHFEVSSLKGYGLYGRP
                     ALVGAAGALIQYLASMRGGSPPEQVVNFRPYDPGSGMVLDSATRRNLGLDELIQTIDR
                     TRTPMGQRAL"
     gene            complement(992..1885)
                     /locus_tag="VFT03_09275"
     CDS             complement(992..1885)
                     /locus_tag="VFT03_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4848378.1"
                     /translation="MNELTWNHRYSRANGIEVHYVRHGRGMPLVLLHGWPEFWYVYRK
                     NVPALAERFDVIVPDLRGFGESEKPGLPDPPSDLLDVLVEDLKGLMDTLGIGRFGLVS
                     HDVGSYVAQAFARKYPGRLLGLFFFHCVYPGIGKRWLDPDSAKEIWYQSFNQQPWAAS
                     LVGRDRTTCMTYFRHFLDHWAGRPGLFDEDLDAWVDNFMKPGNLQGGFNWYIGIDEAR
                     TGLWRHGAPELPKIEVPARFFWGEEDPLVKIAWADRLGEYFSDYSFAAAEGAGHFVHY
                     EKPEAANREIAGFFSTFESSG"
     gene            2090..2881
                     /locus_tag="VFT03_09280"
     CDS             2090..2881
                     /locus_tag="VFT03_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4848379.1"
                     /translation="METHILFFVAAVAAGLINSMAGGGGLITFPLLALVVPPVVADAT
                     SAFALLPAYPSAVWRTRGKLREVPRRWLWLLLIPSVLGGLAGALLLVWTGDRNFTFLV
                     PWLVLGGTVLFVLEPRLSRRSGVAPGNRGLTKAMWPLATAVVFVVAIYGGYFGAGIGI
                     LMISALTLLRMGDVHRVVPLKNLLTGCLRGVAVLVLVIDGVIDWGYGVPMALGGLIGG
                     YLGGTLTGRVNPTALRAVVIVVGFGLAAYYFWTLYGPPGLHFGGE"
BASE COUNT          479 a          978 c         1013 g          551 t
ORIGIN      
        1 cagagcacgc tggcccatcg gggtgcgggt ccggtctatc gtctggataa gctcatccag
       61 cccgaggttg cgccttgtgg ccgagtcaag gaccatcccc gagcccgggt cgtagggcct
      121 gaagttgacg acctgctcgg gcgggctgcc gccgcgcatc gaggcgaggt actggatcag
      181 ggcccccgcg gcgccgacga gagcaggcct cccgtacagc ccgtacccct tgaggctaga
      241 gacctcgaag tggcgcgtca gcgcccgctc tcccgccgag ggctcgaagg tccagcgcgg
      301 agcggaactg atggcggcac gaactttcgg cagatcctcg gcggcggtgc gctcggggac
      361 gaccatctct ctgggcgacc agcgctcgag ctcagccgcg aggtcctcct caggcacctc
      421 cgtcccggtg aactcgcctg tggaggcctc gacgatcgcg acgccaacca tcccgtcgtg
      481 gacgttgatg gcggccaggt agttcgcgtg tccggcggag aggacattgt cctcgatcac
      541 ggtccctggc gtgaggacgc gcgagatctc gcgcgtgaac tgcttgggac gggtagggtg
      601 cggccgctgc tctgctatag ctaccgagtg ccccttcctg agcagcaccg cgacgtgctc
      661 ctgcagagcg tggccaggca ccccggcgag cggaaccctg ccctcgcccg cagcctcgcg
      721 gctcgtaagc cggatggaga gctctcgcga aatggtcttc gcgtcctcct cgaaggtctc
      781 gaagaaggtg cctacctggt agaagaggat cgtgccttgt ggaagctggg ctttgagctc
      841 ggcgtagcgt cctaacatgg agctattcta cggcgagaac ctccgtccgt cagcaccgaa
      901 aactgcccgg gcttctcagc tctccgtctc tcatccatca agacggagca gcgtcctggg
      961 aatccggcgc gaagggcgta tgcatggtgg ttcacccgct tgactcgaag gtcgagaaga
     1021 acccggcgat ctcgcggttg gccgcctccg gcttttcgta gtggacgaaa tgtcccgcac
     1081 cctcggcggc ggcgaaggag tagtcggaga agtactcccc gagcctgtcg gcccaggcga
     1141 tcttgaccag ggggtcctcc tcgccccaga aaaagcgcgc gggcacctcg atcttcggga
     1201 gctcgggcgc cccgtggcgc cacagccccg ttctggcctc gtctatcccg atgtaccagt
     1261 tgaagcctcc ctggaggttt cccggcttca tgaagttgtc cacccaggcg tcgaggtcct
     1321 catcgaagag tcccggccgg cccgcccagt ggtcgaggaa gtgcctgaag taggtcatgc
     1381 aggtcgtcct gtccctgccg accagcgagg ctgcccaggg ctgctggttg aaggactggt
     1441 accagatctc cttggcgctg tcgggatcca gccagcgctt ccctataccc gggtagacac
     1501 agtggaagaa aaacaggccc aggagtctcc ccgggtactt cctggcgaag gcctgggcta
     1561 cgtaggagcc cacgtcgtgg ctcaccaggc cgaagcgccc gatgcctaac gtgtccataa
     1621 gccccttcag gtcctccacc aggacgtcga gtaggtcgct cggggggtcg ggcagcccgg
     1681 gcttctcgga ctcgccgaag ccccgcaggt cagggacgat gacgtcgaag cgctccgcca
     1741 gcgcggggac gttcttgcgg tagacgtacc agaactccgg ccagccgtgc agcaagacta
     1801 gcggcatccc tctgccgtgg cggacgtagt ggacctctat gccgttcgct cgcgagtacc
     1861 gatgattcca cgtcagctcg ttcatccggg caggacatta tggcccgaac cacgaaacgg
     1921 cgcaaaggca tctcggtccg aggcggtcat gccggacttc cgcgaatgcg tctacttcta
     1981 cgccgttggg tgaacaaggg ccgaaaacag gcacacggga gatgctccgg gctcatccag
     2041 gtgagatcat tctcgcgtgg cacatcgggt gaagggaggc tagcgcggca tggagaccca
     2101 tatcctattc ttcgtggccg cggtcgcggc cggcctgatc aactcgatgg cggggggtgg
     2161 cgggctgatt acgttcccgc tgttggccct cgtggtcccg ccggtggtcg ccgacgccac
     2221 cagcgctttc gccctgcttc ccgcctatcc cagcgccgtc tggcgtaccc gcggcaagtt
     2281 gcgcgaggta ccccggcgct ggctgtggtt gctgctcatt cccagcgtgc ttggtgggct
     2341 ggcaggagcg ctgctgctgg tctggacggg cgaccgcaac ttcacgttcc tggtgccgtg
     2401 gctggtgctg ggcggcacgg tccttttcgt gctggagccg aggctgtccc gccgtagtgg
     2461 cgtcgcccct ggtaaccgcg gcctcacgaa ggcgatgtgg ccgctcgcta cggcggtcgt
     2521 gttcgtggta gcaatctacg gcggctactt cggggcgggc atcggcatcc tgatgatcag
     2581 cgccctcacc ctcctgcgga tgggggacgt ccatcgcgtg gtgcccttga agaacctgct
     2641 caccgggtgc ctgcgaggcg tagcggtgct ggtgctcgtg atcgacggcg tgatcgactg
     2701 gggatacggg gtgccgatgg ccctgggcgg cctcatcggt ggttacctgg gaggcacgct
     2761 gaccggcagg gtaaacccga ccgctctacg cgcggtggtc atagttgtcg gattcgggct
     2821 ggcagcgtac tacttctgga ccctctacgg tccaccaggg ctacactttg gcggcgagta
     2881 agccagatca gcaggcccac cgtggaggtg aggagccggg cgacgttaac cctcctcgcc
     2941 cttcgcacct gtctcggggc gcagccagag cgaggcgtag gcgggttgca tggtgtctcg
     3001 caccaccccc acgaggtcgt c
//
LOCUS       DASTUV010000456         6632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1704126, whole genome shotgun sequence.
ACCESSION   DASTUV010000456 DASTUV010000000
VERSION     DASTUV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704126"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1919)
                     /locus_tag="VFT03_09285"
     CDS             complement(240..1919)
                     /locus_tag="VFT03_09285"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU4848380.1"
                     /translation="MAPDIILGPILRHVGSSDATVWVETDAACEVEVRAGGSSHRSPT
                     FTVEGHHYALVTVTGLEPGAVYEYEVRLDGDVVWPEPGSSFPPSEIRPIPPEGDLTLA
                     FGSCRVSVPHEPPYTEKRGVIMRGGVSGKRFSHDALYALALRMRRQPSEEWPDALLLL
                     GDQIYADDISMGTREFIRSRRDTSQPPGEEVADFEEYTHLYWDAWKNPVIRWLLSTVP
                     SAMIFDDHDVHDDWNTSKAWVRQMRSKPWWEERIVGAFMSYWIYQHLGNLSPQELNED
                     DLFRRVREAKDATRTLREFAYEADREVAGSRWSFHRDFGNVRLIMMDSRAGRVLDEDK
                     RSMLDPEEWAWIEDKATGDFDHLLFGTSLPCLLSPGLHHLEAWNEAVCRGAWGSLAAR
                     LGESIRQALDLEHWSAFHSSFAALTQLLRAVGAGDRSEGHPPASMVILSGDVHHGYLA
                     ETDLGDGVESAVYQAVCSPLRNPLGIPERLALHAGWTKTGERIGKALARLARVEEPRV
                     RWRLTHKEPWFSNHVGTLELRGREATLRVEETAPEDGREPRLYTILERRLA"
     gene            complement(1966..2793)
                     /locus_tag="VFT03_09290"
     CDS             complement(1966..2793)
                     /locus_tag="VFT03_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848381.1"
                     /translation="MEQVKATGFEIGDERVAWEGLAASCTPPVRRLGAFLLVGFAYFA
                     AVTAAVVLFYNIFGDTPVEGGGVAVPREAIYATMISSLGLALGGYAVWLVRSLRSYAS
                     FSQILRRGGLDPRRPTARGLGAYSDEQLLALRSRYERLAEGKLWERLGRTFGFNEEDS
                     FSLGPLSVLPGTFEMAALRVEWETNLLLKTGEAMPEISWWTESRSGLLPRQADETLRL
                     VYALRYTADSVRELKRRYGYRTDRWYATVPEGKLFGAVRDYEDSLRIQATLSRRTRR"
     gene            complement(3127..3543)
                     /locus_tag="VFT03_09295"
     CDS             complement(3127..3543)
                     /locus_tag="VFT03_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4848382.1"
                     /translation="MSEGEGGPHAVTHVLIVDDHRSFADALELVLRSTLAGSPRCSCA
                     ASVAEGLRLAQGGPYDLAVIDLMLPDGNGTTVVREIKAQNPCTPVAVVSASEKLSEAL
                     AAGADEAIPKDTGFAQIVTSLARLAGVPHRSSPQDS"
     gene            complement(3641..4321)
                     /locus_tag="VFT03_09300"
     CDS             complement(3641..4321)
                     /locus_tag="VFT03_09300"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HEU4848383.1"
                     /translation="MGELRSVRNQCPLCSHVFLAPHVGSFVTVGREADLCPKFPGRQS
                     DGSRRIRDSITMCPACSFAASENFSELDLTFDERYDIEERLQEDGLLRVFRASPPPWL
                     AFHAAEVCGQERSLRFRELGDLCLRASWVCRKEREHPFESTFQLRALRYFIRALREEG
                     LYGRELSVTTYLIGELNRRLGNHREALNWYVNAERTLGDEDGRDAGLAWLGRLISEQS
                     KLAREQAA"
     gene            4463..4660
                     /locus_tag="VFT03_09305"
     CDS             4463..4660
                     /locus_tag="VFT03_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848384.1"
                     /translation="MDQRKSEAEVALELMRLVLEQAGSRRLSPTRTLALYHLCKEAVA
                     YDPESSVSAEYLEDNLKKLLQ"
     gene            4868..5932
                     /locus_tag="VFT03_09310"
     CDS             4868..5932
                     /locus_tag="VFT03_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4848385.1"
                     /translation="MRATTVLVSSIVALALLAVGCAGGQTGGSQGGGGGGGGETEVRP
                     ALNLDIGGLGDQGFNDSAYAGLQRAKKELNVDTEYLESSAPTDYVDNLTQLADSGYTP
                     VFAIGFLMTDAVNEVSKQYPDTDFAIVDSVSHGPNVASLIFREQEGSYLAGIVAGMMT
                     QQKTEYTNPDDKIVGFLGGQESDLIGRFQAGYEAGVKSVCPDCKVLVQYAGSTPNAFN
                     DPAKGKEISLQQISEGADIIYHAAGNTGAGLFDAAKEKKIFAIGVDLDQAKVFPDAPI
                     LTSVIKRVDNSVFLTIKEASEGKFPGGEVVEFGLDDKGISLAPFGRFDKDVPQKVKDE
                     VEKAKQGIIDGDIKVPSSPQ"
     gene            5945..>6632
                     /locus_tag="VFT03_09315"
     CDS             5945..>6632
                     /locus_tag="VFT03_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848386.1"
                     /translation="MTERETGGGTAGAQAEAPVVLEMRNITKTFGPVKANDNISITLH
                     RGEILGLLGENGAGKSTLMKILYGLYKPDSGSIFIDGEEVEIHDPKDAVSRGIGMVHQ
                     HFTLIPPLTVAENIVLGAEPHKGVALDLEGAIKATEELSERYGLRVDPRARVADLSVG
                     IQQRVEILKTLYREARILVLDEPTAVLTPQETEDLFSVLKELVADGLSIILITHKLGE
                     LLGVSDQITII"
BASE COUNT         1186 a         2166 c         2184 g         1096 t
ORIGIN      
        1 agaggaactg cccctcaggg tggatgtgcc agttcgtgcg cgcccccggc acgaagagca
       61 cgcggaacag ccccgcgcct ggcctgggga tcgagctcgc gtccatccag acgtctccgg
      121 taaaccagtc ctccggtccg cgccgggtct ccatgtccct ggacgaaaca acttgcatgg
      181 gtgcctccct ttgccgttca ggtctcaggc atcaggttac aggtcctgaa gcctcgtctt
      241 catgccagac ggcgctcgag tatcgtatac agccgtggct ctctgccgtc ctccggtgct
      301 gtctcttcca ctctcagggt ggcttcgcgg ccgcggagct cgagtgtgcc gacgtggttg
      361 gagaaccagg gctccttgtg cgtcagccgc cagcggaccc gcggttcttc gacgcgggcc
      421 aggcgagcca gggccttgcc tatgcgttcg cccgtcttcg tccagcccgc gtgcagcgcc
      481 aggcgctcgg gtatgccgag cgggttccgc agaggggagc agacggcctg atagaccgcg
      541 ctctctacac cgtcgccgag gtccgtctcg gccagatagc catggtgaac gtcgccggag
      601 aggatgacca tcgaggcggg gggatgaccc tccgatcggt cgcccgcgcc gactgcccgc
      661 aggagctgcg tgagtgcggc gaaggagctg tggaaggcgg accagtgctc gaggtcgagg
      721 gcctggcgga ttgactcgcc gagtctcgcc gcgagggaac cccaggcacc ccggcagacg
      781 gcttcgttcc aggcttccag gtggtgcagg cccggggaca ggaggcaggg gagggatgtg
      841 ccgaagagga ggtggtcgaa gtctccggtg gctttgtcct cgatccaggc ccactcctcc
      901 gggtcgagca tggagcgttt gtcttcgtcg agcactcgtc cggcgcgcga gtccatcatg
      961 atcaggcgca cgttgccgaa gtcgcggtgg aagctccagc ggctgcccgc gacctcgcgg
     1021 tcggcctcgt aggcgaactc gcgcagggtg cgggtcgcgt ccttcgcctc ccgcacccgt
     1081 ctgaagagat cgtcttcgtt taattcctgt ggggagaggt tgccgaggtg ctggtagatc
     1141 cagtacgaca tgaaagcccc gacgatccgc tcctcccacc acggcttgga gcgcatctgg
     1201 cgcacccagg ctttcgaggt gttccagtcg tcgtggacgt cgtggtcgtc gaagatcatg
     1261 gcagagggca cggtcgagag gagccacctt attaccgggt tcttccaggc gtcccagtac
     1321 aggtgcgtgt actcctcgaa gtcggcgacc tcctcgccag gaggctggct cgtgtcgcgc
     1381 cgcgagcgga tgaactcacg ggtacccatg gagatatcgt cggcgtagat ctggtccccc
     1441 agcaacaaca gcgcgtccgg ccactcctcc gagggttggc ggcgcatcct gagggcgagg
     1501 gcgtagaggg cgtcgtgcga gaacctcttt ccgctcacgc cgccgcgcat gatcacgcct
     1561 cgcttctctg tgtacggcgg ctcgtgaggg accgagacgc ggcaggagcc gaaggcgagg
     1621 gtaaggtcgc cttccggggg gatgggccgg atctcggacg gcgggaagga cgagcccggc
     1681 tccggccaca ctacatcgcc gtccagcctg acctcgtact cgtagaccgc tcccggctcg
     1741 aggccggtga ccgtcaccag agcgtagtgg tgtccctcga ccgtgaaggt tggggagcgg
     1801 tgcgaggatc ctcctgcgcg aacctcgacc tcgcaggccg catccgtctc gacccagacg
     1861 gtggcgtccg aggagccgac gtggcgcagg atgggaccga ggatgatatc aggcgccatc
     1921 tcgggtaact acccattgtt gtacgttttc acccgtcgtc cggtgctagc ggcgggtcct
     1981 gcggcttaaa gttgcctgga tgcgtaaaga gtcctcgtag tcgcggacgg ccccgaagag
     2041 cttgccctca gggactgtgg cgtaccagcg gtcggtccgg tagccgtagc ggcgcttgag
     2101 ctcccgcacc gagtcggctg tgtagcggag cgcgtagacg aggcgcaggg tctcgtcggc
     2161 ctggcgcggc aggaggccgc tcctactctc ggtccaccag gagatctccg gcatagcctc
     2221 ccctgtcttg aggagcaggt tcgtctccca ttcgacccgt agagcggcca tctcgaacgt
     2281 gccgggcaac acgcttaggg gacccagcga gaaggagtcc tcctcgttga acccgaaggt
     2341 gcggccgagg cgctcccaca gcttcccttc ggcgagccgc tcgtagcggg agcgcagtgc
     2401 gaggagctgc tcgtcggagt atgccccgag gcccctggct gtcgggcgcc tggggtcgag
     2461 cccgccgcgg cggagtatct gggagaacga agcgtaggag cgcaggctcc tcacgagcca
     2521 cacggcgtag ccgccaaggg cgagacccaa gctcgagatc atggtcgcgt agattgcctc
     2581 tctggggacc gcgacccccc ctccttcaac gggcgtatcg ccgaagatgt tgtagaaaag
     2641 gacgacagcg gcagtgaccg ccgcgaagta ggcgaagccg acgagcagga acgcccccag
     2701 ccgcctcact ggcggggtac acgacgcagc caggccctcc caggccacac gctcgtcgcc
     2761 tatctcgaac cccgttgcct ttacctgctc cacgccgacc agtatacctg catcacactc
     2821 ctccggatgg gtgagatatc accccgcgcg gccgcggcaa cgcgtgctgc acagcgcgcc
     2881 ggcagggaga tccgcccgta aatcatgctt cgatccgccg ggcataacac agaaggccgg
     2941 tgaagcgatc acggcttcac cggcccgtgc cacgacgcta cgccgtggcg tttgtgtaac
     3001 agagtgctct atgtcaagct accctgtcca tcggtcctta cctcctactc gaaggtccgt
     3061 tccgacatcg aaaatcttac caaattctca cgccgactcc agcgtctcct ccctgagggt
     3121 gggatatcag gagtcctgag gactcgaccg gtgggggacc cccgcaaggc gcgcgaggga
     3181 cgtgactatc tgcgcgaagc cagtgtcttt tgggatcgcc tcgtcggccc ccgctgctag
     3241 cgcctcggag agcttctcgc tggcgctcac cacagccacc ggcgtgcacg ggttctgagc
     3301 ctttatctcc cgcacgacag tagtcccgtt accgtcgggc aacatcaggt cgatcaccgc
     3361 caagtcgtac gggccgccct gtgcgagccg caggccctcg gcgaccgaag cggcgcagga
     3421 gcatctcggg gaaccggcga gggtggagcg gagcacgagc tccagggcat cggcgaagga
     3481 tctatggtcg tccactatca ggacgtgcgt cacggcgtgc ggtcctccct cgccttcact
     3541 catccagggc tcattctaag ccaaacagta ctctgtgcgt cattgggcgg atcaccagcg
     3601 cattgtgcga gtagcggatc gcgagccgcc actgcacgct ctaagcggcc tgttctctgg
     3661 cgagcttgct ctgctcggag atcaggcgtc ccagccacgc gaggccggcg tcgcgcccgt
     3721 cttcgtcccc tagcgtgcgc tcggcgttga cgtaccagtt gagggcctcg cggtggttgc
     3781 cgaggcgcct gtttagctcg cctatgaggt aggtggtcac ggagagctcg cgcccgtaga
     3841 gaccctcctc cctgagggcg cggatgaagt atcggagagc cctgagctgg aaggtgctct
     3901 cgaaggggtg ctcgcgctcc ttgcggcaga cccacgaggc tcggaggcac aggtcaccga
     3961 gctcgcgaaa gcggagactc cgctcctggc cgcagacctc ggcggcgtgg aaggccagcc
     4021 acggcggggg actcgcgcgg aagaccctga ggagcccgtc ctcctgcaag cgctcctcga
     4081 tgtcgtagcg ctcgtcgaag gtcaggtcga gctcggagaa gttttcgctg gcggcgaagg
     4141 agcacgcggg acacatggtt atggagtccc ggatccgccg cgacccgtcg gactggcgcc
     4201 cggggaactt ggggcagaga tccgcttccc tcccgaccgt gacgaaactc ccgacgtgcg
     4261 gcgccaggaa gacgtggcta cataaagggc actggttgcg gacgctccta agctccccca
     4321 cggatgccct tcccgccgcg cgggatgccg agaacgagcg aggattccca gacaaccaaa
     4381 gcgctttcga gtttaccaca gcgccgcgcc cccgccggta taatgcccgg cggagcttcc
     4441 cggaaaggcg gtcgtccctg ctatggacca gcgcaagagc gaggccgagg tcgccctgga
     4501 gctgatgagg ctcgtcctcg agcaggccgg cagccgcagg ctctccccga ctcgcaccct
     4561 cgcgctctat cacctctgca aggaggccgt cgcctacgac cccgagagtt cggtctccgc
     4621 cgaatacctc gaggacaacc tcaagaagct cctccagtag cgccgggagg gcggtcttcg
     4681 gtgctggcgg ccacgcgcct ggccgaactc gcgcagctac tccagctcga ggctgggatc
     4741 ttcttgcgaa cgcacggcgg acgacctctc cttatgcgac aatattcgct actctatggg
     4801 ttgctattga acggcgaatc ggtagggagc cggctgcccg gtgggggtcc acgaagaagg
     4861 aggtctcgtg cgcgcaacaa cggtgttggt ctcgtcgatc gtggccctgg cgttgctggc
     4921 ggtcggttgc gcgggggggc agaccggggg aagtcagggc ggcggtggag gaggcggcgg
     4981 tgagaccgag gtacgcccgg cgctgaacct cgacatcggc ggcctcggcg accagggctt
     5041 caacgactcc gcctacgccg ggctacagcg ggccaagaag gagcttaacg tcgataccga
     5101 gtacctcgag tccagcgccc cgaccgacta cgtggacaac ctcacccagc tcgccgactc
     5161 ggggtacact cccgtcttcg ccatcgggtt cctcatgacc gacgctgtca acgaggtctc
     5221 caaacagtac cccgacaccg acttcgccat cgtggactct gtctcacacg ggcccaacgt
     5281 ggcgagcctg atcttcagag agcaggaggg gagctatctg gcgggaatcg tagccgggat
     5341 gatgacccag cagaagaccg agtacaccaa cccggacgac aagatagtcg gcttcctcgg
     5401 cgggcaggag tccgacctca tcggcaggtt ccaggccggc tacgaggccg gagtcaagtc
     5461 ggtgtgcccc gactgcaagg tcctggtgca gtacgcggga tctacgccca acgctttcaa
     5521 cgaccccgcg aagggcaagg agatcagcct gcagcagatc agcgagggcg cggacatcat
     5581 ctaccacgcc gccggcaaca cgggcgcagg cctcttcgac gcggcgaagg agaagaagat
     5641 cttcgcgatc ggcgtggacc ttgaccaggc caaggtcttc cccgatgctc ccatcctgac
     5701 gtccgtgatc aagagggtgg acaactccgt cttcctgacc atcaaggaag ccagcgaagg
     5761 caagttcccc ggcggcgagg tcgtcgagtt cgggctcgac gacaagggca tcagcctggc
     5821 gccgttcggc cgcttcgaca aagacgtccc tcagaaagtc aaggacgagg tcgagaaggc
     5881 caagcagggg atcatagacg gcgacatcaa ggttcctagc agcccgcagt agaggggaca
     5941 ccgggtgacc gagagggaga caggcggggg gacggcaggg gcgcaggcag aggccccggt
     6001 cgtcctcgag atgcggaaca tcaccaagac attcggaccc gtaaaggcca acgacaacat
     6061 ctccatcacg ttgcaccgcg gcgagatcct cgggctgctg ggggagaacg gcgcggggaa
     6121 gtccaccctg atgaagatcc tctacgggct ctacaagccg gactcgggct cgatcttcat
     6181 cgatggcgaa gaggtcgaga tccacgaccc gaaggacgcc gtatctcgcg gcatcgggat
     6241 ggtccaccag cacttcaccc tgatcccacc cctcactgtg gccgagaaca tcgtcctcgg
     6301 cgccgagccc cacaagggcg tcgccctgga tctcgaaggg gctataaaag ccaccgagga
     6361 gctctcggaa cgctacgggc tgcgggtgga cccgagggcg cgcgtcgccg acctgtcggt
     6421 ggggatccag cagagggtcg agatcctcaa gaccctctac cgggaggccc gcatcctcgt
     6481 cctcgacgag ccgacggccg tcctcacccc gcaggagacc gaggacctct tctccgtgct
     6541 caaggagctc gtcgccgacg ggctctcgat catcctgatc acccacaagc tcggtgagct
     6601 actcggcgtg agcgatcaga taaccatcat cc
//
LOCUS       DASTUV010000457         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1157066, whole genome shotgun sequence.
ACCESSION   DASTUV010000457 DASTUV010000000
VERSION     DASTUV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157066"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFT03_09320"
     CDS             <1..614
                     /locus_tag="VFT03_09320"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4848387.1"
                     /translation="GVTVLVIGAGAFAAVNGSGGIGKTYETLVNNPNQTEDMGQRLAS
                     LDIGFRDDYWSVGWEAWKEHPLTGTGAGTFKYTWLQNRPNSQGVQQVHNLYLEQGTET
                     GVFAFLALAGFVVTFVGYLARATWRSRGERRLLLAGFTSALGVYLVSSFLEWHWYIPG
                     STLFFFFLAGITVKFACSEEWVAPEVEVRRADDGGSPDERPQP"
     gene            626..1423
                     /locus_tag="VFT03_09325"
     CDS             626..1423
                     /locus_tag="VFT03_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848388.1"
                     /translation="MAGARRAGGSASLSRLLGGLHVDLGDHRSSVFLAGSGRSGTTWV
                     SGLINHRNAYRFVFEPFRQDKVGICENFGRKQYLRSGDRREEYLGPARTILTGGLRSL
                     WTDRFNEKLVARRRLIKDIRANLLLGWMRANFPGMPIVLLLRHPCAVVASQLALGWRD
                     VLSETMEQEELVEDFLTRPEHELQRLFSFLGEDFDERVYPLLDRPSPLSREGEAASVD
                     AWQRSVTGRQLERAVEILGLFGLDRVYGEAPMPDPSGAHALMSLVRR"
     gene            complement(1523..1651)
                     /locus_tag="VFT03_09330"
     CDS             complement(1523..1651)
                     /locus_tag="VFT03_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848389.1"
                     /translation="MSPFSPEGLPSPARRILDAALRRVDQRGVGLARPVVSTKTVG"
     gene            1728..2552
                     /locus_tag="VFT03_09335"
     CDS             1728..2552
                     /locus_tag="VFT03_09335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4848390.1"
                     /translation="MARDLLKLAGPDPVVVAYPPTRTTLDLISGLRPRAVLYDCSDDY
                     EQFPGAPGDIADTERELLQLADLVSCTSRRLLEKVRPARPDAFPSGPAVDYERFAVLQ
                     DDKRSKEIRTVCFFGSVSRERTSVAALRAIAEAGFEVRLLGDLGRVGRNLLKTPGIDF
                     RSEVPHARLPAALAGVDAFVLPYEINGLTAGISPAKTYECLATGKPVVASPLPALKEL
                     AGHVYLADRPEEFVEVLRELGELETEERVRARIELSRDNAWGTRFREIEEALGRVL"
     gene            2570..3307
                     /locus_tag="VFT03_09340"
     CDS             2570..3307
                     /locus_tag="VFT03_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU4848391.1"
                     /translation="MNVLGVGVDPLTVGDLQAEVGRLVRDRKRGLVLNANAHCLNLCY
                     EDPKLRDFLNGAEVVFCDGAGVMLAARILGRRIPVRITYADWAWQLADFAEAQNFSLY
                     FLGARPGVAQAAARKLKQRYPGLNVVGVHHGYFDRSENEAVVQKINAAAPDILLVGLG
                     MPLQEYWLMENRHRLDAGVALTGGAVFDYVSGRLRRGPRLLVSSGFEWLARLLIDPRR
                     LWRRYLVGNPLFLLRVVRQRLGVREKM"
     gene            complement(3538..4035)
                     /locus_tag="VFT03_09345"
     CDS             complement(3538..4035)
                     /locus_tag="VFT03_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848392.1"
                     /translation="MAGLVTGAALGAVGSFLALDARLAIGSILAVVAIAVGALEFFGR
                     RVQPPQFDCETPQRWVHKGPMGWAIRNGLALGVGATSRIGFWLWYVVPLGAFLVGDPI
                     FGAVIYGTYSLIRALGATVILLGMIRLKTDISDWLIEHYGEARLLAAGQLVFLGVATA
                     IVVGL"
BASE COUNT          712 a         1381 c         1420 g          735 t
ORIGIN      
        1 tcggcgtgac ggtgctcgtg atcggggccg gagcgttcgc cgctgtgaac gggtccggcg
       61 ggatcggaaa gacctacgag acgctcgtga acaaccctaa ccagaccgag gatatggggc
      121 agaggctcgc ctccctcgat ataggcttca gggatgacta ctggagtgtg ggctgggagg
      181 cgtggaagga acatcctctg acgggcacgg gtgccggaac cttcaagtac acctggctcc
      241 agaacaggcc caactcccag ggcgtccagc aggtccacaa cctgtacctc gagcagggca
      301 ccgagaccgg cgtgttcgct ttcctggcgc tagccggctt cgtggtcacg ttcgtcggct
      361 acttggcgag ggccacctgg cggtcgcgcg gagagcgccg gcttctgctc gctggcttca
      421 cgtcagcgct cggggtctat ctggtgtcct cttttctaga gtggcactgg tacattcccg
      481 gctcgacact gttcttcttc ttcctcgccg gcataaccgt gaagttcgcc tgtagcgagg
      541 agtgggtagc ccccgaggtc gaagtccggc gtgcggacga cgggggttct ccggacgaac
      601 gaccacagcc gtagcggcgg tccgcgtggc gggagcgaga agagcgggag ggagcgcgtc
      661 cctctcccgg ctgctcggcg ggctccacgt ggacctgggt gatcaccgga gctcggtctt
      721 cctagcaggt agcgggcgca gcgggacgac gtgggtatcc gggctgatca accaccgcaa
      781 cgcgtaccgc ttcgtcttcg agcctttccg tcaggacaag gttggtatct gcgagaactt
      841 cggacgaaag cagtatctga ggtccggcga ccggcgcgag gagtacctcg ggcctgcaag
      901 gaccatactc accggcgggc tcaggagtct ctggacggat cggttcaacg agaagctcgt
      961 cgcgagacgg cgcctgatca aggacataag ggccaacctg ctgctcggtt ggatgcgcgc
     1021 gaacttcccc ggcatgccga tcgtactcct cctccgccat ccctgcgccg tcgtcgcgtc
     1081 acagctcgcg ctagggtgga gagacgtcct ctcggagacg atggagcagg aggagctggt
     1141 cgaggacttc ctcacgcgtc ccgaacacga gctccagcgc ctgttctcgt tcctcggcga
     1201 agatttcgac gagcgcgtct accccctcct ggatcgtccc tcgcccctga gccgcgaggg
     1261 cgaggccgct tccgtggacg cctggcagcg ctccgtgacc ggccgccagc tcgagcgagc
     1321 cgtcgagatc ctcggtctct tcgggctcga tcgcgtctac ggcgaggccc ccatgcccga
     1381 cccttcgggc gcgcacgcct tgatgagcct ggtacggcgg tgatcccctg gacaagccgc
     1441 cggtaataat cctatcgggc gtccgctggg gcttcctctg gcagcgccac cacgcactcg
     1501 ccaccctctt cgccagcgcc ggctacccga ccgtcttcgt cgagaccacg ggcctcgcca
     1561 accccacacc gcgttggtcc actctcctca aggcggcgtc caggatccgg cgcgccgggg
     1621 aaggcagacc ctccggagag aaggggctca ccgtctacgc cccgctcgcg ctcccgccga
     1681 cctacagggc gttccggggg ttgaaccgga ggtacttcct tccgtgggtg gcgcgggacc
     1741 tcctgaagtt agccggaccg gatcccgtcg tcgtggcgta cccgccgacc aggaccacgc
     1801 tggacttgat ctctggtctt cgaccccgag ccgtcctcta cgactgctcg gacgattacg
     1861 agcagttccc gggcgcgccc ggagacatcg cagacaccga acgcgagctc cttcagctgg
     1921 ccgacctcgt ctcgtgcacc tccaggcgcc tgctagagaa ggtgaggccc gcgagacccg
     1981 acgccttccc gagcggcccg gccgtggact acgagcgttt cgccgtcctg caagacgata
     2041 agcgcagcaa agagatccgt accgtctgct tcttcggcag cgtgagccgg gagcggacga
     2101 gcgttgctgc gctgcgggct atcgcggagg caggcttcga ggtgcggctt ctcggggacc
     2161 tgggccgcgt cggcagaaac ctcttgaaga cgcccggcat agatttccgg agcgaggtgc
     2221 cccacgcgcg gctcccggcg gccctcgctg gtgtggacgc ctttgtgttg ccctacgaga
     2281 tcaacggcct cacggcgggt atctcgccgg ccaagaccta cgagtgcctg gcgaccggta
     2341 aacccgtcgt agcgtccccg cttccggctc tgaaggagct ggccgggcac gtgtacctcg
     2401 cggatagacc ggaggagttc gtggaggtct tgcgggagct cggtgagctg gagacggagg
     2461 agagggtgcg ggcaaggatc gagctctccc gcgacaacgc ctgggggacg cgcttcaggg
     2521 agatagagga ggccctgggg cgggtcctgt aaagcacccc gcggaccggg tgaacgtgct
     2581 gggggtgggt gtggacccgc tgacggtcgg ggatctgcaa gcggaggtcg gacgcctggt
     2641 acgggacagg aaacgcggcc tcgtgctgaa cgcgaacgcc cactgcctca atctgtgcta
     2701 cgaggatccc aaactgcgag atttcctgaa cggcgcggag gttgtcttct gcgacggggc
     2761 gggtgtgatg ctggcggcga ggatcctggg ccggcggata ccggtcagga tcacctacgc
     2821 cgactgggcg tggcaactgg cggatttcgc cgaagctcag aacttctcgc tctacttcct
     2881 cggcgcccgc cccggcgtgg cgcaggcggc ggcgaggaag ctgaagcagc gttaccccgg
     2941 tctgaatgtg gtcggcgtcc accacggcta cttcgaccgc tcggagaacg aggcggtcgt
     3001 gcagaagata aacgccgccg ctccggacat cctgctcgtc gggctcggga tgccgctcca
     3061 ggagtactgg ctgatggaga accggcacag gctcgacgcc ggcgtcgccc tcactggcgg
     3121 cgccgtcttc gactatgtct ccgggcggct gcgccgcggt ccgcgacttt tggtcagcag
     3181 cggcttcgag tggctggcgc gtctacttat cgacccccgc cgcctgtggc gaagatacct
     3241 cgtcggcaat cccctttttt tgttgcgggt cgtgaggcag cgcctcgggg tgcgggagaa
     3301 gatgtaggat ccctgcgcgc ggggacgcac cctgccgcta cctccggcgg gccgtctcgc
     3361 agcgacggcg tctacaggtt tggctgggaa gcgaggcggg gatcaagggt tcccggatat
     3421 cagctactga agaaccggca gagcttcctc tgggctgatc cttcaacgcg ggcaggactc
     3481 gcgctggtcg gtgtggcgta cctggcaggc gatggcgtcc ggaccatggg tttttgctta
     3541 tagaccgacg acgatagctg ttgccactcc gaggaagacc agctgtcctg ccgctagcag
     3601 ccgggcttcg ccgtagtgct cgatcaacca gtccgagatg tccgttttga gccgtatcat
     3661 gccgagcagt atcaccgttg cccccagagc ccggatcagg ctgtacgtgc cgtagatgac
     3721 ggcgccgaat ataggatctc cgactagaaa ggctcccaaa ggaaccacgt accacagcca
     3781 gaacccgatc cggctcgtag ccccgactcc tagcgccagg ccgttccgga tcgcccatcc
     3841 cattggccct ttgtgcaccc agcgctgggg cgtttcacag tcgaactgtg gaggttgtac
     3901 gcggcggccg aagaactcaa gagcgcctac ggctatcgcc acgaccgcga ggatgcttcc
     3961 tatggcgagg cgggcgtcga gagcaaggaa gctccctacg gcgccaaggg ccgctcccgt
     4021 gacgagaccg gccaccgtcg acgagagcga gtaaacgatc gccttgctga tccaactgct
     4081 gcctgcctgg ttccgatacc cgtcggaact cgcgatcccg tagaccgcgt tagggcttca
     4141 cggaagcgtt tgctggacga tcgccgtcac cgccgcagag acgacgagca gcgcaaagat
     4201 ccagcccatc cccccgaagc cgagcgagaa cgccccggca agggcgac
//
LOCUS       DASTUV010000458         5061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1585674, whole genome shotgun sequence.
ACCESSION   DASTUV010000458 DASTUV010000000
VERSION     DASTUV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585674"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFT03_09350"
     CDS             <1..234
                     /locus_tag="VFT03_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4848393.1"
                     /translation="DAPEETRLSGREAEVLMLAARALSNREIAESLHLSETTVKRHLS
                     NVYEKMGVRSRGEAARKALSEGWISAWDVSRED"
     gene            443..787
                     /locus_tag="VFT03_09355"
     CDS             443..787
                     /locus_tag="VFT03_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848394.1"
                     /translation="MRILIANTPLMYRESLALAVHRHNPDFEVMIADPASLDGEAERF
                     GPHTLIRDDDGIEVGSPDGVVCWVGIIIDDHLKARISLDGKVSEIHEVSLYELLAALD
                     EAARFVSENGIR"
     gene            complement(820..1485)
                     /locus_tag="VFT03_09360"
     CDS             complement(820..1485)
                     /locus_tag="VFT03_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848395.1"
                     /translation="MPADPIKVLLADDHTLFRNGLAGLLASYGGMEVVGSTPNGEDAV
                     ALVERTRPDVVLMQVQIPFERAKDILTKMRAVSPPPKVVIVTMFESPRYLRELMDLGA
                     SAYLIKSASTQHLIGAIRAAVFDPQGENVVVGMPRGMLEEARNGSGGMLSARELEILL
                     LAARGLSNREIGENLHIAEATVRRHLANTYSKMEVSSRGEATRKALQEEWITVSDVTE
                     DID"
     gene            1751..2128
                     /locus_tag="VFT03_09365"
     CDS             1751..2128
                     /locus_tag="VFT03_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848396.1"
                     /translation="MIDNAIERRTDATTRFETSWLIVRDDVGAGGGPPEVLTLGVEGD
                     GTILPVFSFEEEALLFLRLCGFSSGWHASKSGIADLASVLSDTCPEVRRVALDPIPEI
                     GLHGPHDLVSLSREEFVGLLATG"
     gene            2298..2486
                     /locus_tag="VFT03_09370"
     CDS             2298..2486
                     /locus_tag="VFT03_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848397.1"
                     /translation="MCYGLYKTIKTDYREERRQMQETLAYDREMKLKQMKEIMSGPPR
                     PVTATPAGRTEPKPNGRQ"
     gene            2518..2769
                     /locus_tag="VFT03_09375"
     CDS             2518..2769
                     /locus_tag="VFT03_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848398.1"
                     /translation="MTRTFNLDTTVGPASLLASARRAAHENGATLVGDERSGSFSHAM
                     LAGGYRMAGRTVTVTITDKHWVIPWPLVEARLEQLVGPA"
     gene            3227..4285
                     /locus_tag="VFT03_09380"
     CDS             3227..4285
                     /locus_tag="VFT03_09380"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU4848399.1"
                     /translation="MQIIQTALAACVAWFLAVLILGIDRPTFAPIAAVIALGLAVGER
                     VRRAVELTLAVAFGVAMADLLVSLVGVGAVQAGAVVLLAMGLAVFLGGGDLGVKEAAI
                     SALIIMITFRSSQAGFPIERFLEALIGGGTALLINALLPVNPERMVEDAAFPVFAESA
                     AVLEEVADALEDGDARRAQRAYVKAREIDARVAGLKEAVAAGRETARLAPPRRRSLGH
                     MELYTRAADQIDLTVRDVRALARAALSVVQPEAEDPAPQRLLAAIRGLARATEALAAY
                     LQTSGDPPDETRRLALEAATEASTLLEEHEDLASNLGVNALVDQIHSSAVDLIGGTGM
                     DRSEALQALEEATGRASW"
     gene            complement(4447..4980)
                     /locus_tag="VFT03_09385"
     CDS             complement(4447..4980)
                     /locus_tag="VFT03_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4848400.1"
                     /translation="MNKDQTLTLPSGVEISFRVVRPDDAPALQRLHARCSERTIYLRF
                     FGSMKNLSDEQARYFASTDGVDHFGLVALDPEDPNEIIAVVRYARKPGNERAEYAALV
                     EDSWQGQGVGAALTRRLIDEAQGNGVASFYALVKGENKRMLNVLRHLDLPEQERVEDG
                     EKMVEIGLFYEESQRTG"
BASE COUNT          910 a         1547 c         1688 g          916 t
ORIGIN      
        1 gacgcgcccg aggagaccag gctttccggc agggaggcgg aggtcctgat gttggcggcc
       61 cgtgctctct ccaaccgcga gatagctgaa agcctccatc tctccgagac caccgtcaag
      121 cgccacctct ccaacgtcta cgagaagatg ggcgtgcgct cccgcgggga ggcggcgagg
      181 aaggcgctct ccgagggttg gatctccgcc tgggacgtct ccagggaaga ctagcgtcct
      241 cgacgagcgt tctccgcccg tcgcctcgcg gagaatgatt gcatcattcg gaccattttt
      301 ccgccgaacg ccgaggagta gacctgcact ggtgtagact agctgggacg cggctgccca
      361 agcggtcgcc caggccaatt tagtggacct acgtctgtgc tccttttccg gcggatgttt
      421 ctccgcggaa gaggagcaag caatgcgaat cctgatagcc aacacgccgc tgatgtacag
      481 ggaatctctg gccctggcgg tccaccgcca caacccggac ttcgaggtga tgatcgccga
      541 ccccgcgtcc ttggacgggg aggccgagcg cttcgggccg cacacgctca tccgggacga
      601 cgacgggatc gaggtaggct cgcccgatgg tgtcgtgtgc tgggtcggca tcatcatcga
      661 cgatcacctc aaggcccgca tcagcttgga cgggaaggtc tcggagatcc acgaggtgag
      721 cctgtacgaa ctgctcgccg ccctcgacga ggccgctaga ttcgtttccg agaacggcat
      781 ccgctaacgg cagcggctaa cggtgggtga gaggacccgc tagtctatgt cctcggtgac
      841 gtcgctgacg gtgatccact cctcctggag ggctttccgc gtcgcctccc cgcgggagga
      901 tacctccatc ttggagtagg tgttcgcgag gtggcgcctg accgtcgctt cggcgatgtg
      961 taggttctcg cctatctcgc ggttggagag gccgcgggca gccagaagca ggatctccag
     1021 ttcccgggcg gagagcatgc cccccgatcc gttcctggcc tcctcgagca ttcccctcgg
     1081 catgcccacc accacgttct cgccttgtgg gtcgaagacc gccgcgcgga tcgcgccgat
     1141 caagtgctgg gtcgaggcgc tcttgatcag gtaggcactc gctccgaggt ccatgagctc
     1201 ccgcaggtag cgcgggctct cgaacatcgt gactatgacc accttgggtg gcggcgagac
     1261 ggcgcgcatt ttagtcagga tgtccttcgc cctctcgaac gggatctgca cctgcatgag
     1321 gacgacgtcg ggtctggtcc tctcgaccag ggccacggca tcctcgccgt tgggcgtgct
     1381 gcctacgacc tccatgcccc cgtaggaggc caacaagcca gcgagcccat tccgaaacag
     1441 tgtgtggtcg tcggcaagta gtaccttgat cggatcggcc ggcaagctgc gtccctttct
     1501 cgtaggaccc gaaccagtgg taccaattct agcttatcag ggcggttcac tccaccagga
     1561 ggcgtaccca ggcgacggag ccaggtttcc tacaccttcc ggtccgttct gtgcatacat
     1621 ttatgcattt atccttggcg gtgttcttta cgagtgttcc cgcgcgacgt caccatgtat
     1681 cttgaggtac ctgcggcctg cgcgagaccc cgacaaacgg tgtcccggcc gcaagagaga
     1741 gggtggtcgg atgatcgaca acgctatcga aaggaggacc gatgcgacaa cgaggttcga
     1801 aacttcctgg ttgatagtga gggacgacgt cggtgccgga ggtggcccgc cggaggtcct
     1861 cacgctcgga gtcgagggcg acgggacgat cctgcccgtc ttcagtttcg aagaggaagc
     1921 gctgctcttc ttgcgcctct gcggcttctc gagcggatgg cacgccagca agagcgggat
     1981 tgcggacctc gcctcggtcc tctctgacac ctgcccggag gtccggcgcg tcgccctcga
     2041 cccgatcccc gagatcggcc tccatggacc gcacgacctc gtcagcctgt cgcgagagga
     2101 gtttgtcggg ctgctcgcca ccggatgatg attctcagca cgggccccct gcgccggaga
     2161 ggtttttggc cggcgcgaga agcggcgcgg atctttaggt aaggcatcgc ggtgcgacca
     2221 gtggcaacca cgaaagaagg tgtgtatgac gaacacgaaa gcgggcaagg acgccgttcg
     2281 ggatactcat ggttcccatg tgctacggcc tgtacaagac aatcaagacg gactaccgcg
     2341 aggagcgacg ccagatgcag gagacgcttg cctacgaccg ggagatgaaa ctgaagcaga
     2401 tgaaggagat catgtcgggc cccccgcggc cggtcaccgc aacccccgcg ggccggacgg
     2461 aaccgaagcc gaacggtaga cagtgatcta gcgcaggaga acggagccga gaatacgatg
     2521 acgagaacct ttaacctgga cacgacggtg ggccctgctt cgctgctggc cagtgccagg
     2581 cgggccgccc acgagaacgg cgccacccta gtcggcgacg agcggtcggg gagcttctcg
     2641 cacgccatgc tcgcgggcgg gtaccgcatg gcgggccgaa cggtcaccgt cacaataacc
     2701 gacaaacact gggtgatccc gtggcccttg gtggaagccc ggctggagca gctcgtgggc
     2761 ccggcttgag cagacgcccg gtttgaagac gtgcgcagta cgacgcggtg atcccccacc
     2821 ggcgcggaga gggtgaggac gggcgggttc ggtgtccggg ccctgcaggg acgggttgca
     2881 accgcagacg ccgggtacaa ctatggcgct aaactgacca gagggacgaa ggcgtacaaa
     2941 gaggcccgaa caaggctgag cgggaggcgt ccttccagaa gcagcagggc gagcgggagg
     3001 cgaaggcgcg aggagcagca ggtacgcgag gaggagtagc gacaaaggcg tcggcacgtt
     3061 aggcaggaag agcgggatct ccggctcggc gggccgcacg tcgccgggta agctagaaaa
     3121 ggagcgcagg atggacacgc gctaagggat gttcaggcaa gtgagggcgg tcggctccgt
     3181 ccgggtccgc ttgcgtgccg gctgggtccg tatgcggtcc gggtggttgc agatcattca
     3241 gacggcgctt gccgcctgcg tcgcgtggtt cttggccgtc ctcatccttg ggatagacag
     3301 gcccacgttc gccccgatag cggcagtcat cgcgctcggg ctggcggtgg gcgagcgcgt
     3361 ccgccgtgcc gtggagctca cccttgcggt ggcattcggc gtcgccatgg ccgatttgct
     3421 cgtatcctta gtcggagtag gggccgtgca ggccggagcg gtggtgctgc tggcgatggg
     3481 tcttgcggtc ttcctgggtg ggggagatct gggcgtaaag gaagccgcca tctcggcgct
     3541 gatcattatg attaccttcc gctcctcgca ggccggcttc ccgatcgagc gctttctgga
     3601 ggcgctgatc ggcggtggta ccgccctgct aatcaacgcg ctgctcccgg taaaccccga
     3661 gcgcatggtc gaggatgccg cgttccccgt cttcgccgag tcggcagccg tgctcgaaga
     3721 ggtggcggat gccctggagg atggcgacgc caggcgggcc cagagggcgt acgtgaaggc
     3781 cagggagatc gacgcgcggg tggctggatt gaaagaggcc gtcgctgccg gacgggagac
     3841 cgctcgcctc gctccgcccc ggcgaaggag cctcgggcac atggagctct acactagggc
     3901 ggccgaccag atagacctga cggtgcgcga cgtgagggcg ctggcccgcg ccgcgctctc
     3961 cgtggtccaa cccgaagccg aagatccagc cccccaacgc ctcctagctg cgatccgcgg
     4021 cctggcccgg gcgacggagg ccttggccgc ctacctgcag acctccggag atcctcccga
     4081 cgagacgcgc cggttggccc tggaggccgc cacagaggcc agcacgctct tggaggagca
     4141 cgaggacctg gcgagcaacc tgggtgtcaa cgcgctcgtc gaccagatcc actcctcggc
     4201 ggtcgacctc atagggggca ccggcatgga tcgttcggag gccctgcaag ccctggaaga
     4261 ggccacggga cgcgcatctt ggtagcgagc gtgctatggg cgaacttgct gctgggaacg
     4321 catctccaag gtcggggact accccggcat tgggccctag cccttattca ccagagtgcg
     4381 gagaaggatg attctcggaa actcggaggg cttgaacgac ataaaagggg gacaaggcta
     4441 gggctcttac cccgtgcgtt ggctttcttc gtagaacagc ccgatctcca ccatcttctc
     4501 gccatcttcc actcgctctt gttcgggaag atcgaggtgc cgcagaacgt tcagcattcg
     4561 tttgttctca ccctttacga gcgcgtaaaa tgatgcaacc ccgttgcctt gcgcttcgtc
     4621 gatcagccga cgggtcaaag ctgcccctac cccctggccc tgccaactat cctcgacgag
     4681 cgccgcgtac tcggctcgtt cgttacctgg tttgcgggcg tagcgtacga cagcgatgat
     4741 ctcgtttgga tcttcgggat ctagggcaac caggccgaag tgatccacgc catcagtgct
     4801 agcgaagtag cgggcctgct cgtccgatag atttttcatc gaaccgaaga agcgcaggta
     4861 gatggtgcgt tcgctgcatc gagcgtggag ccgctgcaag gcgggagcgt cgtctggcct
     4921 aacgacgcga aaggagatct cgactccgct aggtagggta agcgtttggt ctttgttcat
     4981 gccgattatc ctatatctct ctggtgtctt tattgcctag gaaggggtgt tctcgggact
     5041 tcgggcgtaa gggcttcgct g
//
LOCUS       DASTUV010000459         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1478539, whole genome shotgun sequence.
ACCESSION   DASTUV010000459 DASTUV010000000
VERSION     DASTUV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478539"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1588
                     /locus_tag="VFT03_09390"
     CDS             164..1588
                     /locus_tag="VFT03_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848401.1"
                     /translation="MMGGRERRRWAALALLCVAQFVDVLDVNAVIVALPAMGRDLGFS
                     PEDLQWVVTAYVLFFGGFLLLAGRLSDLYGRRRMFVVGLAVFSASSLACGLAGSPLAL
                     VAFRAVQGLGAAIVAPAALSIISTTFTGGRERNLAMGVWTAVAAGGGAAGLVLGGFIT
                     DSLGWPWIFFVNVPIGVLGIALSFVLLGAGRGERGARRLDAWGAVTVTAGLFLLVYGL
                     TRAEEAGFGSPLTLAALALALVLLATFLFVERSVADPLVPLRVLRSPDLSGSALVAFA
                     NTATTSSVGIIAILYLQEVLAYSPSKAGLLGLPFSLSVVMGSFLGSRLTGSLGARRTM
                     VLGLLGICGATLLISGISAEGGLPYVLSNAVLAGLSLGCSAVASTTRGTAAVEAEERG
                     LASGVLNSSAQIGTALGLAALITVAAARTGALAGGEEASAAAVVEGFRAAFYVAAGLA
                     VAGALVPLLLMRGKEQAPLEKGEA"
     gene            1640..>2369
                     /locus_tag="VFT03_09395"
     CDS             1640..>2369
                     /locus_tag="VFT03_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848402.1"
                     /translation="MRSRSITGRSLWAARMAVLGIFFLNGFGFGSWAVRIPAVQERLD
                     LGEGLLGLALLGMAAGSLVSMPLAGGLVSRLGSRPVVGATGLGFFLALPLLAWSPSLA
                     LLMPALAFAGASGGSLDVSMNAHAVAVEKGQGRPIMSSFHAAFSLGGLAGAAVGGAVA
                     AWGVGVAPHLAGVSALSLAAFALAYLQMLPADEDRGQSGEPAFARPTRALAGLGVISF
                     CVLLGEGAMADWSAVYLKGTLGTGP"
BASE COUNT          256 a          768 c          874 g          471 t
ORIGIN      
        1 gtcagcctgt acaagatcgg ggacaagtac ttcgtccagg acggcaacca ccgcgtcagc
       61 gtggcccgtt accagggagt agagatgatc gacgccgagg tcgtggagct tatgacgccg
      121 gcttcggtgc gaggacgggt atccgcattc cgctaacgcc ggaatgatgg gaggtcggga
      181 gaggcgccgg tgggcggcgc tggcgttgct gtgcgtggcg cagttcgtcg atgttctgga
      241 cgtgaacgcg gtgatcgtcg ctctgcccgc gatggggcgg gacctcggtt tctcgccgga
      301 ggatctgcag tgggtggtca cggcctacgt gctctttttc gggggattcc tgcttctggc
      361 ggggaggctc tcggacctct acgggagacg ccggatgttc gtggtggggc ttgccgtgtt
      421 tagtgcttcc tcgctcgctt gcgggcttgc gggctcgccg ctggcgctcg tggcgttccg
      481 ggccgttcag ggtctagggg ctgccatcgt cgcccctgcg gcgctctcca taatctcgac
      541 caccttcacc gggggccgcg agcggaacct cgccatgggc gtatggacgg cggtggcagc
      601 cggcggtggc gccgccgggc tcgtccttgg cggcttcatc accgacagcc tggggtggcc
      661 ctggatcttc ttcgtcaacg tgcctatcgg tgtgttgggc atcgcgctct ccttcgtcct
      721 gctcggggcg ggtcgtgggg agcgaggggc gcgccggctc gacgcgtggg gagcggtgac
      781 ggtcacggcg gggctattcc tgctggtgta cggcctcacc cgggccgagg aggcaggctt
      841 cggctcgccc ctcacgctgg ccgctttagc tttagccctg gtcctgctgg ctaccttcct
      901 cttcgtcgag cggagcgtgg cggatcctct ggtcccgctc cgggtccttc gctctccgga
      961 cctttccggt tctgccctgg tggcctttgc gaacacagcg acgacgagct ctgtgggcat
     1021 catcgccatc ctctacctgc aggaggttct cgcctactcc ccgagcaagg ctgggcttct
     1081 tgggctgccc ttcagcttgt cggtcgtaat gggctccttc ctgggctcca ggctcacggg
     1141 aagtctcggc gcgcgacgca ccatggtgct gggactgcta ggcatctgtg gcgcgacgct
     1201 gcttatctcg ggcatatcgg cggagggcgg cctgccctac gtgctctcga acgccgtcct
     1261 cgccggtctg tccctgggct gctcggccgt cgcctccaca acccgcggga ccgcggcggt
     1321 agaagctgag gagcgtgggc tcgcctccgg cgtcctgaac tcctcggcgc agatcggcac
     1381 tgccctcggc cttgccgccc tcatcacggt cgccgcggcc cgcaccgggg ccctcgccgg
     1441 tggcgaggag gctagcgccg ccgctgtggt cgaggggttc agggccgctt tctacgtcgc
     1501 cgcggggctc gccgtggcgg gggcgctagt cccgctgttg ctgatgcgcg gcaaggagca
     1561 ggcgcccctc gaaaaagggg aagcgtaaaa tcgcctgcta tcctccaggt ttgtctggct
     1621 gcatggcggg aggtatctct tgaggtccag gtctatcacc gggcgttcgt tgtgggcggc
     1681 ccggatggcc gtgctcggga tattcttcct caacggcttc gggttcggga gctgggccgt
     1741 gcgcatccct gcggtgcagg agaggctcga ccttggcgag ggattgctgg gtctcgcgct
     1801 gctcgggatg gcggccggct cgctcgtctc gatgccgctg gccggggggc tggtgtcccg
     1861 cctgggcagc cgccccgtgg tcggagcgac ggggctggga ttcttcctgg cgctgccgct
     1921 cctggcctgg tccccgagcc tcgcgttgtt gatgccggct ctcgccttcg ccggggcctc
     1981 cggtgggtcg ctggacgtct ccatgaacgc ccacgcggtc gcggtcgaga aggggcaggg
     2041 gcgtccgatc atgtcttcct tccacgcagc gttcagcctc ggcgggctcg cgggagccgc
     2101 cgtcggcggc gccgtcgcgg cgtggggcgt cggcgtggcg ccgcacctcg caggcgtctc
     2161 cgctctgtct ctggcggcgt tcgccctcgc gtacctgcag atgctgcccg cggacgagga
     2221 cagaggacaa agcggggagc cggcgttcgc caggccgacg cgggcgctgg ccgggctcgg
     2281 ggtgatctcc ttctgcgtcc tgctcggcga gggcgcgatg gccgactgga gcgccgtcta
     2341 cctgaagggc acgctcggga ccggaccgg
//
LOCUS       DASTUV010000460         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_334343, whole genome shotgun sequence.
ACCESSION   DASTUV010000460 DASTUV010000000
VERSION     DASTUV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334343"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VFT03_09400"
     CDS             <1..1196
                     /locus_tag="VFT03_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4848403.1"
                     /translation="AGEALRGKDSAEKHSGRRLRRAIYLVLLAVLIGLLVSYWSWVDA
                     QARAVMVISSVLDAPVLTPAAEAVSGDPRFEDIPVAGNPTLVARPAGEGPWPAIFLVN
                     GTVREGRKLPEVRNLAEGFARAGYLVVVPDLPGLTEAKITPDTARETVEVAREVSARP
                     DVEDGEVALVGVSTGATLALLAAERASLEGRVSVVAGIAPYSDIRTVVNIATTGHYRR
                     SDGELIRYEADSFLPYVIAHSLVAALPPGEDRRTLSGELASIDREDPDPLSVLRARRT
                     DDLGPGARSVVGLLANRKPERFAALYADLPDDVRHDLEELSPLAGTGRIRVPVELATG
                     PHDKYFPPSQSYALERVAPQRRVTVTEALDHAKLEVSPGDVPAFATFDAFVVRSLRTA
                     RTAES"
     gene            complement(1202..>2058)
                     /locus_tag="VFT03_09405"
     CDS             complement(1202..>2058)
                     /locus_tag="VFT03_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU4848404.1"
                     /translation="CGHPGLDLLAVTTVAGNVPIEKTTRNTLRVMSLVGCTYVPVGVG
                     ASEPLERPLHTAEDIHGESGLDGAEEIPDASFEPDERVAVALIADTLRASPEPVTLVP
                     VGPLTNIATFLREHPDLKDNVAHISLMGGSMGHGNTTPAAEFNIYVDPEAAREVFESG
                     LPITMSGLDITHQAGAGPDERERLRATGRVGGVVAGFLDFFAATYERVFGFDAPPLHD
                     PVAVAAVLEPGLLKTRPMRVDIECESELTRGETVCDFYGVTGRAPNAEVGVDLDREGF
                     FKLLYGTL"
BASE COUNT          354 a          679 c          660 g          365 t
ORIGIN      
        1 cggcgggaga ggcgttgcgg ggtaaggatt cggcagaaaa acactccggt agaaggctcc
       61 gtcgcgccat ctacctcgtg ttgctcgcgg tactgatcgg tttgctcgtc tcgtactggt
      121 cgtgggtgga cgcgcaggcg cgcgccgtca tggtgatctc ctccgttctc gacgcgcctg
      181 tactgacgcc tgcggccgaa gccgtgagcg gcgaccctcg tttcgaggac atccccgtgg
      241 ctggcaaccc cactctcgtc gcgaggcctg cgggagaggg accgtggccg gctatattcc
      301 tggtcaacgg gactgtgcgc gagggaagga aactgcccga ggtaaggaac ctggccgagg
      361 gcttcgcacg ggccggctac ctggtcgtcg tgcccgacct gcctgggctg acggaggcta
      421 agatcacccc cgatactgcc cgtgaaaccg tcgaggtcgc ccgcgaggtc tccgcacgac
      481 cggacgtcga agacggagag gtggccctgg tcggggtctc gacgggcgct acgctagccc
      541 tcctcgcggc agaaagggcg agcctcgaag ggcgggtctc ggtcgtcgcc ggcatcgcac
      601 cttactcgga catccgcacc gtcgtgaata tcgcaacgac cggccactac cgccggtccg
      661 acggagagct gatccgctac gaggcagatt ccttcctccc ttacgtcatc gcccactccc
      721 tcgtcgccgc actgcctcct ggcgaggacc ggcgtacgtt gtcaggcgaa cttgcgagca
      781 tagaccgcga ggaccctgac ccgctctccg tcctccgcgc acgccggacg gacgacctag
      841 gaccgggggc caggagcgtc gtcgggctgc tcgcgaaccg taagccggaa cgcttcgctg
      901 cgctctacgc cgatctcccc gatgacgtgc gccacgacct cgaagagctc tcgccgctcg
      961 ccggcacggg aaggatacgt gtcccagtcg agctggccac cggtccccac gacaagtact
     1021 tccctccctc tcagtcgtac gcgctagagc gcgtcgcgcc ccagcgcaga gtgacggtaa
     1081 cagaggcgct cgaccacgct aagctcgaag tctcaccagg agatgttccg gccttcgcaa
     1141 ctttcgacgc cttcgtagtc cgctcgctac gcacggcccg tactgctgaa agctgagcgc
     1201 attacagcgt tccgtacagc agcttgaaga aaccttcgcg gtcgagatcg acgccgacct
     1261 cggcattcgg cgccctgcct gttacaccgt agaagtcgca gacggtctcg cctcgggtta
     1321 gctcgctctc gcactcgatg tcgacccgca tcgggcgagt cttcaggaga cctggttcga
     1381 gtactgctgc gacggcgacg ggatcgtgca gcggcggggc gtcgaagccg aatacacgtt
     1441 cgtaggtggc agcgaagaag tccaggaacc cggccactac gccacccacc ctacctgtgg
     1501 cccgcagccg ctccctttcg tcaggaccgg caccggcctg gtgtgttata tcgaggcccg
     1561 acatcgttat gggcagaccg gactcgaaga cctcgcgggc cgcctcggga tcgacgtaga
     1621 tattgaactc ggcggccggg gtcgtattgc catggcccat gctccctccc atgagggaga
     1681 tatgcgcaac attgtcttta aggtcgggat gctcgcgcag gaatgtggcg atgttagtga
     1741 gcgggccgac agggacgagg gttactggct caggagaggc ccttagggtg tccgcgatca
     1801 gggctacagc caccctttcg tcgggttcga agctggcgtc ggggatttcc tcagccccgt
     1861 ccagcccgct ctcgccgtga atatcctcgg ccgtgtggag cggtctttcc aggggttccg
     1921 aagcgccaac gcccacaggc acgtacgtac accctacgag ggacatcacc cgcagggtgt
     1981 tgcgcgtggt cttctcgatg ggaacgttgc cggcgactgt cgtaacggcg agcagatcaa
     2041 gacccgggtg tccgcagg
//
LOCUS       DASTUV010000461         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1551533, whole genome shotgun sequence.
ACCESSION   DASTUV010000461 DASTUV010000000
VERSION     DASTUV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551533"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFT03_09410"
     CDS             complement(<1..192)
                     /locus_tag="VFT03_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stilbene synthase"
                     /protein_id="HEU4848405.1"
                     /translation="MSEPGPRILSVATSLPPYRVGQGEAKRFARGMFAEAHRDFERLI
                     PIFDNVHVENRNFCVPVEWF"
     gene            complement(189..1007)
                     /locus_tag="VFT03_09415"
     CDS             complement(189..1007)
                     /locus_tag="VFT03_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU4848406.1"
                     /translation="MDFLEVVKRRRTTNGPFLPDPVSEEHKRLLVEVAAMAPSHFNSQ
                     PWRFVLVDERDTIEEVSRISGESMTRLLEEGTFWKRYRPYFRFSEEEMEEKRDGIHFD
                     QMPGVLKPFRRQIFTNTGQAVMNRFGVPKTLGEDNRKLVAGSPLLLAVLLDRTEYRPG
                     ELSGFYSVFGMGAAVENVWLTTAAIGMGIQFVSTPMEIPENWQKLRRLLKVPDDLELM
                     AVYRLGYVPQEQRRPTIDWSSRQRKRVSQYVFRNTCEQPEPEPDVSQEAGPATR"
     gene            complement(1017..1325)
                     /locus_tag="VFT03_09420"
     CDS             complement(1017..1325)
                     /locus_tag="VFT03_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848407.1"
                     /translation="MIFYAASALFVANFALGVLVQFGIVDTRPFRWLHHALFFAVFAS
                     AALAVAVGFLQGAPYRWALLPVLALFLVLPRVRAGTRGHAALASCAMIFYATGFVSLL
                     "
     gene            1464..>2098
                     /locus_tag="VFT03_09425"
     CDS             1464..>2098
                     /locus_tag="VFT03_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEU4848408.1"
                     /translation="MENGRPAAYITGVGVVSPVGIGRKRFWSSLLACESGASRITHFD
                     PEGFEVRIAAECTDFDAGDFMDRKLAARTDRFAQMALASAKLALEDAGAWDELNQRPE
                     RVGLILGSGIGGVMSMETTQATIDSRGPARVNPFSVTKIMPNSAAAHIAIQLGVQGPS
                     STGALACSCGTDMMGIGYDLIRRGDADVVICGASEATITPVIVAGFIAMRA"
BASE COUNT          392 a          661 c          713 g          332 t
ORIGIN      
        1 gaaccactcc acgggcacgc agaagttgcg gttctcgacg tggacgttgt cgaagatggg
       61 gatcaggcgc tcgaagtccc tgtgcgcctc ggcgaacatg cccctcgcga accgctttgc
      121 ctcgccctgc cccacgcggt agggcggcag ggaggtcgcg accgagagga tcctgggacc
      181 gggctcgctc aacgtgtggc cggcccggcc tcctgcgaga cgtccggctc cggttccggt
      241 tgctcgcagg tgttgcggaa gacgtactgg gagactcgct tgcgctggcg gctcgaccag
      301 tcgatggtgg ggcggcgctg ctcctggggc acatacccga gccggtagac ggccatcagt
      361 tcgaggtcgt caggcacctt caggagcctc cgtagcttct gccagttctc tgggatctcc
      421 atcggcgtcg agacaaactg gatgcccatg cctatggccg ccgtggtgag ccagacgttc
      481 tcgacggccg cccccatccc gaagacagag tagaagcccg agagctcgcc agggcggtac
      541 tccgtcctgt ccagcaggac ggccaggagc agaggagagc cagcgacgag cttgcggttg
      601 tcctcgccca gggtcttcgg aactccgaag cggttcatca cggcctggcc cgtgttggtg
      661 aagatctggc gcctgaacgg cttgaggacg ccgggcatct ggtcgaagtg gataccgtcg
      721 cgcttctcct ccatctcttc ttcggagaaa cggaagtagg gacggtagcg cttccagaag
      781 gtgccctcct ccagcagccg ggtcatgctc tcgccgctta tcctcgagac ctcctcgatg
      841 gtgtccctct cgtccacgag gacgaaacgc cagggctggc tgttgaagtg agagggggcc
      901 atggcggcaa cctcgaccaa aagacgcttg tgctcctcgc tcaccgggtc aggcagaaag
      961 gggccgttgg tcgtcctgcg tctcttcacg acctcgagga aatccaagtc agcctcctag
     1021 agcagggata cgaacccggt agcgtagaag atcatggcgc aggaggcgag cgctgcgtgc
     1081 ccccgcgtgc cagcccgcac ccgcggaagt accaggaaga gcgccaggac cggcaggaga
     1141 gcccagcggt agggcgcccc ctgcaagaac cccacagcga cggcgagcgc agcggaggcg
     1201 aagacggcga agaacaaagc gtggtgcagc cagcggaaag gtctggtgtc cacgatcccg
     1261 aactgcacca gcacgccgag cgcgaagttg gccacgaaga gcgccgacgc tgcgtagaag
     1321 atcaagccat actcctcatc cccggaggtt atagcatagg tggttcgcgc cggacgcgac
     1381 gaaccgcgcc cgcacaagtc gtagcgaggc ggcggtgtta agattcgtag ttcgacggtg
     1441 aggcgctttt gctagggggt gagatggaga acggacgccc ggcggcttac ataacggggg
     1501 taggggtggt cagccccgta gggatcgggc gcaagcgatt ctggagttcg ctgctggcct
     1561 gcgagagcgg cgccagccgg ataacgcact tcgaccccga gggcttcgag gtgaggatcg
     1621 cggccgagtg cacggacttc gacgcgggcg acttcatgga ccgcaagctg gcagcccgga
     1681 ccgaccggtt cgcccagatg gccctcgcct cggccaagct cgcgctcgag gacgcaggcg
     1741 cgtgggacga actgaaccag cggccagaaa gggttggtct catactcggc agcggcatcg
     1801 ggggcgtgat gagcatggag acgacccagg cgacaataga cagcaggggt ccggccaggg
     1861 tcaacccgtt ttcggtcacc aagatcatgc ccaactccgc cgctgcgcac atagccatcc
     1921 agctcggcgt gcagggaccg tcgtccacgg gggcactggc gtgcagctgc ggcaccgaca
     1981 tgatgggtat cggctacgac ctcatccgcc gcggcgacgc cgacgtcgtc atctgcggcg
     2041 cctcggaggc taccatcacc cccgtcatag tcgccgggtt catcgcgatg cgcgcgat
//
LOCUS       DASTUV010000462         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_851866, whole genome shotgun sequence.
ACCESSION   DASTUV010000462 DASTUV010000000
VERSION     DASTUV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851866"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..754)
                     /locus_tag="VFT03_09430"
     CDS             complement(95..754)
                     /locus_tag="VFT03_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU4848409.1"
                     /translation="MDVYDEVWERFVRERRLEFGGHTDPQWREGHALSASLVIPVQGS
                     RFRERLEPLRDALRPFPFVSLHPDHFLHITVLFMGFVVEKPERENEVSWVRLEEIEAS
                     ARHNLSDFPAFTVGLANLNAFPGAAFVEVHEGEMLDSLRRTLCEACGLEKSSGPPHLT
                     LAYFHAPDGTPAPEDLIRAIARYRDWPVGELAVGYVDITVLDLRIEYPEPKVLARIKL
                     E"
     gene            complement(808..1587)
                     /locus_tag="VFT03_09435"
     CDS             complement(808..1587)
                     /locus_tag="VFT03_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEU4848410.1"
                     /translation="MGDRARTMKYRRRVRLYGFIAVSFLAFLGALILGLSIFGIDLLG
                     TTDGPIGRMVAPAIPDSAEQVQRAAQDEADYVARAKQAAAEQAAKQEAEAAKQRAERR
                     AEQEKASPTPAVPSDTTMYLTVPKLGLYDIPVLEGTDEAVLSQGVGHMPGTGYPWSEG
                     SNTYIAGHRLGYPGTVSDHVFYNLPSLAVGDEVILEDSLGQTYTYQVSEILEVSPSDL
                     SVTSPTGADIVSLQTCIENYGDYWTEGPNWFARYIVRADRV"
     gene            complement(1705..2811)
                     /locus_tag="VFT03_09440"
     CDS             complement(1705..2811)
                     /locus_tag="VFT03_09440"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848411.1"
                     /translation="MAQDLREAITAVADRPGPVFSAYVSVNAALPENQRRAYLVRMRD
                     AMNDLGVPEEVQSQVREHTEAETHPRARTLVLFAAEDGLLEDYRLHVDLPESFRWGDP
                     NVAPLTLVMDEYEPYGAAVLDAERFRYFVVSPLARPQEGEEARANGFREVDVHPSQPY
                     PRGGTDFEPASRRTEAHVHRFYNELGKLTRDITFREGVRRLILAGPRERTAEFRKVLP
                     NELKDRIVAEEHVDLGAPEGELIDRLEAIREQAEHEREKELLAEIRERGVRGPDETIA
                     ALQEENRVYHLAALWDLEGEIRWCDNDHLAIRDITAEECPFCGRKTRLRPLVDVLVDL
                     AAARGARLDFMLGENERTDTLREEFGGIAGLTRF"
BASE COUNT          482 a          956 c          984 g          548 t
ORIGIN      
        1 ggcgtgctga caagcggaag ccgaaggctg gagcgtgctg acaagcggag gcgaagccgg
       61 agcgtgctga caagccgacc gcagggaggc gtgcttactc gagctttatc ctcgcaagaa
      121 ccttgggctc cgggtattcg atgcggaggt ccaggactgt tatgtctacg tacccgactg
      181 caagctctcc gacgggccag tcccggtagc gcgctatggc gcggatgagg tcctcggggg
      241 ccggcgtgcc gtccggcgcg tggaagtagg cgagcgtcag gtgcggtggc cccgaggact
      301 tctcgagccc gcaggcctcg caaagagtcc ggcgcaaact gtccagcatc tcgccctcat
      361 gcacttcgac gaacgcggct ccggggaagg cgttgaggtt ggcgagcccg accgtgaagg
      421 ccgggaagtc agagaggtta tgccgcgcgc tcgcctcgat ctcctccagc cgcacccatg
      481 agacctcgtt ctctcgttct ggtttttcca cgacgaagcc catgaagagc accgtgatgt
      541 gcaagaagtg gtcggggtgc agcgagacga acgggaaagg gcgcaacgcg tccctcaagg
      601 gctcgagccg ctcgcggaag cgcgacccct ggaccgggat cacgagcgag gccgagagcg
      661 cgtgcccctc gcgccactgg ggatctgtgt gcccaccgaa ctccaggcgg cgctcgcgga
      721 cgaaccgctc ccagacctcg tcgtagacgt ccaccctcga gactctatca gaaggttaga
      781 gtaggccggc tacgcaggag tagaggctta gacccgatcg gcccggacga tgtaacgggc
      841 gaaccagttg gggccctccg tccagtaatc gccgtagttc tcgatgcagg tctgcagcga
      901 gacgatgtca gctccggtcg gcgaagtcac cgagaggtcg ctcggcgaga cctcgagtat
      961 ctcgctgacc tggtaggtgt acgtctggcc gagagagtct tcgaggatga cctcatctcc
     1021 caccgccagg ctcgggaggt tgtagaagac gtggtcgctc acggtgccgg ggtagccgag
     1081 ccggtggccc gctatgtacg tgttggagcc ctccgaccag gggtagcccg tgccgggcat
     1141 gtgaccgacg ccctgcgata ggacagcctc gtcggtcccc tcgaggaccg gaatgtcgta
     1201 gagccctagc ttggggactg tcagatacat ggtggtgtcc gacgggactg caggagtcgg
     1261 cgatgccttc tcctgctcgg cgcgccgctc cgccctctgt ttcgcggctt cggcctcctg
     1321 cttggccgcc tgctcggcgg ccgcctgctt ggcgcgtgcc acgtagtccg cttcgtcctg
     1381 cgccgcacgc tgcacctgct cggccgagtc cggtatggcg ggagctacca tcctgccgat
     1441 cgggccgtcg gtcgtaccca ggaggtctat cccgaagatg ctcaacccga ggataagggc
     1501 gccgaggaag gcaaggaaag agaccgcgat aaagccgtaa agcctcacac gccgcctgta
     1561 tttcatggtc cgcgccctgt cgcccataca ttaatcgtag cagcagatat agtggaccgc
     1621 catacgaatt gtgaaaattt tttaagattc gcgaggtttt ttcgtggagc cggcgctggc
     1681 tccctgcgag gctgcggtgc ctgctcagaa gcgggtaagt cctgcgatgc ccccgaactc
     1741 ctcgcgtaat gtgtcggtgc gttcgttctc gccgagcatg aagtccaggc gggcgccacg
     1801 ggcggcggcg aggtccacca ggacgtcgac caggggcctg aggcgggtct tccggccgca
     1861 gaagggacac tcttcggccg ttatgtcccg tattgcgaga tggtcgttgt cgcaccaccg
     1921 gatctcaccc tccagatccc agagtgccgc gaggtggtag acgcggttct cctcttgcag
     1981 ggccgcgatg gtctcgtcgg gtccacggac gccgcgctcc cggatctccg cgagcagctc
     2041 cttctcgcgc tcgtgctcgg cctgctccct gatagcctcg agccgatcga ttagctcgcc
     2101 ttcgggggcg ccgaggtcta cgtgctcctc ggcgacgatg cggtccttga gctcgtttgg
     2161 gagtaccttg cgaaactccg cggtcctctc tcttgggccg gccaggatca gacgcctcac
     2221 cccctcgcgg aaggtgatgt cccgggtgag ctttccgagt tcgttgtaga agcggtgcac
     2281 gtgggcctcc gtcctacggc tcgccggttc gaagtccgtg ccgcccctgg ggtacggttg
     2341 gctggggtgg acgtcaactt ccctgaagcc gttggccctg gcctcctcgc cctcctgggg
     2401 ccgcgcgagc ggcgagacca cgaagtagcg gaacctctcg gcgtcgagga ccgcggctcc
     2461 gtacggctcg tactcgtcca tgactagcgt caggggggcc acgttgggat cgccccaccg
     2521 gaacgactcg ggcaggtcga cgtgcagcct gtagtcttcg agtaacccgt cctccgctgc
     2581 gaagagcacg agcgtgcggg cccgcgggtg ggtctcggcc tcggtgtgct cccggacctg
     2641 actctgcacc tcctcgggca cgccaaggtc gttcatggcg tccctcatgc gcaccaggta
     2701 agctcgcctc tggttctccg gcagggcggc gttgacgctc acgtaagccg agaataccgg
     2761 ccccggacgg tccgccacgg cagttatggc ttctctgaga tcctgcgcca tccttcgaac
     2821 acctctggtc tgttgtgcgg agtagtgctc ccaaacagga ttccccccgg ccaccgccgc
     2881 gtaaacatgc gccggacgtg ctagcctcgt cccgcaacag gcttgccgag gaggtgaggg
     2941 gttggtcgag caaggcggat acccgctccc
//
LOCUS       DASTUV010000463         3066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1048956, whole genome shotgun sequence.
ACCESSION   DASTUV010000463 DASTUV010000000
VERSION     DASTUV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048956"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3066)
                     /locus_tag="VFT03_09445"
     CDS             complement(<1..>3066)
                     /locus_tag="VFT03_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU4848412.1"
                     /translation="LDGLLGSIEHPVTGEKTRISEYYKELLFDEYEGRGVPTIYNGSN
                     SPEKVADGRIFIHDAFTTYGLEPAEHYEEHDGPNQSIMCVSCNTTNITGILLRLTDVP
                     GMSGMKIRVMTHRKANDQYVGEDKQSVNYQGMAFDFHYHHWDDARLFYDRIKDESVRR
                     RLGAMFALDNVREGDAAAWAISTHATGQAATEFHTGVVHLSGELHGKPLDAEELKIEL
                     SRRESESAQLIEFPGKVDARHLLETLHYRIGIRNLYVQPFTVTQDGDDLAIVFFTPHL
                     FNVKPNNLVAALNRDGLIPNTISGTEIGTNIVSEALGLGHQQAGLVRYYGTRSEQILA
                     GAQPIYFFGGEQVEGMSIPSWEERRQLLGGKGAGMTEMAAITYEDPVSGEEAGLPVPS
                     GYTITTEQARRYFENGGRLPEDLVQAIDEYQARLESVTGKTLGDTDDPLLVSVRSGAA
                     VSMPGMMDTILNLGLNHQSVEGLARRSGDRRFAWDCYLRFTEQYSDIALGVDDETHLK
                     PLRKAVLEQRGVEDVSGLELEDLEHLTWEYQHAVLEVTGQPFPQDTRSQLLAAIEAVF
                     ESSYSERAVFFRRVNKIPETAVSAVSVVSMVFGNRGEDGATGVCFTRSPLTGDNVVTG
                     EYLTNAQGEDVVAGIRAGKPIEEMENDPKLEGAYRQLYAAQEALERHFRDPQDVEFTI
                     EGGQLFILQTRALGGRTGRASLKIVTDMVEEGVISPREAVIKYGDPKGLNELLQPAFD
                     PEEEEKARQDGRLVATGIPASAGAATGEVTLHPEQAEVLSQRGHDIILVRHETSPEDV
                     SGLHASNGVLTARGGVVSHAAIVARGMGKSAVVGAESVDIDERRGVLHIGEEAIAPGE
                     VISIDGTTGEVFLGALPVVPSPIKARLAGKEVEGEEMYERLQRLLGWAREHKAVEIRC
                     NTETEEDLEVALALGAEGVGLARTEHYMLGPEERRTAFTALILAQSEQERERALSSIY
                     PVMERAAGMIFRLLVGKPATIRLFDPVPNEFLPRSDVELARVAGFMELS"
BASE COUNT          457 a         1021 c          999 g          589 t
ORIGIN      
        1 aggagagctc catgaacccc gccacgcggg ctagctcgac gtcgctacgc ggcaggaact
       61 cgttcggcac gggatcgaag agccgtatcg tggccggctt gcccaccagc agacggaaga
      121 tcatacccgc agcccgctcc atgacagggt agatcgagct cagggctctc tcgcgctctt
      181 gctcgctctg ggccagtatc agggcggtga acgcggtgcg gcgctcctcg gggccgagca
      241 tgtagtgctc cgtgcgagcc aggccaacac cttcggcgcc cagagcgaga gcgacttcga
      301 ggtcctcctc ggtctcggtg ttacaccgga tctcgacggc cttgtgctcg cgggcccagc
      361 cgagcagccg ctgtaacctc tcgtacatct cctcgccctc cacctccttc cccgccagcc
      421 tggcctttat gggcgagggg accaccggca gagcgccgag gaatacctcg cccgtggtgc
      481 cgtcgatgga gattacctcg cctggcgcta tcgcctcctc gccgatgtgc agcaccccgc
      541 ggcgctcgtc gatatcgacg ctctccgccc cgacgaccgc cgacttgccc attccccgcg
      601 cgacgatggc ggcgtgcgag actaccccgc ccctggcggt aaggaccccg ttggaggcgt
      661 ggagaccgct cacgtcctcc ggcgaggtct cgtgccggac caggatgatg tcgtggccgc
      721 gctgcgagag gacctccgct tgctcggggt ggagggtgac ctcgcccgtg gcggcgccgg
      781 ccgaggccgg gatgccggtg gcgacgagcc tgccgtcctg gcgcgccttc tcctcctctt
      841 cggggtcgaa ggccggctgg agcaactcgt tcaggccctt ggggtcgcca tacttgatca
      901 ccgcctcccg cggcgagatg acgccctcct cgaccatgtc ggtgacgatc ttgagcgagg
      961 cccggccggt gcgccctccc agagcgcggg tctgcaggat aaagagctgg ccaccctcga
     1021 tggtgaactc cacgtcctgc gggtcccgga agtggcgctc cagcgcttcc tgggcagcgt
     1081 agagctggcg gtaggccccc tccagcttcg ggtcgttctc catctcctcg atgggcttac
     1141 cggcccggat gccggccacc acgtcctcgc cctgcgcgtt ggtcaggtac tcgcccgtga
     1201 caacgttatc tcctgtcagg gggctgcggg tgaagcagac gccggtggcg ccgtcctcac
     1261 cccggttgcc gaagaccatg gagaccacgc tcaccgccga gacggccgtc tctgggatct
     1321 tgttgaccct ccggaagaag acggcacgct ccgagtagct cgactcgaag accgcctcga
     1381 tagccgccag cagctggctg cgcgtgtcct gcgggaaggg ttgtcccgtg acctcgagga
     1441 cggcgtgctg gtactcccag gtgaggtgct ccagatcttc gagctccaga cccgagacgt
     1501 cttcgacgcc ccgctgctcc agcacggcct tgcggagcgg cttgaggtgc gtctcgtcgt
     1561 ctactccgag cgcaatgtcg gagtactgct cggtaaaacg caggtagcag tcccaggcga
     1621 agcgcctgtc gccgctccgg cgggcgagtc cctcgacgct ctggtggttg aggccaaggt
     1681 tgaggatagt gtccatcatg cccggcatgg agacggccgc ccccgagcgc acggagacca
     1741 gcagggggtc gtcagtgtcg cccagagtct tgccggttac ggattcgagg cgggcctggt
     1801 actcgtcgat tgcctggact aggtcctccg gtagccggcc gccgttctcg aagtagcgcc
     1861 gggcctgctc ggtcgtgatg gtgtagccag acggcacggg cagcccggcc tcctccccgc
     1921 tcacagggtc ctcgtaagtt atggcggcca tctcggtcat gcccgcgccc ttgccgccca
     1981 ggagctgccg gcgctcctcc cagctgggga tgctcatccc ctcgacctgc tcaccgccga
     2041 agaagtagat gggctgcgca ccggcgagta tctgctcgga gcgggtcccg tagtagcgaa
     2101 cgagtcccgc ctgctggtgg ccgagaccca gcgcctcgct cacgatgttc gtcccgatct
     2161 ccgtgcccga gatggtgttg gggatgagcc cgtcgcggtt gagcgccgcc accaggttgt
     2221 tcggcttgac gttgaagagg tgaggagtga agaagactat cgccaggtcg tccccgtcct
     2281 gggtaacggt aaagggctgg acgtagaggt tgcggatgcc gatgcggtag tggagcgtct
     2341 cgagcaggtg cctggcgtcc accttgccgg ggaactcgat gagctgggcg ctctcggact
     2401 cgcgcctgct cagctcgatt ttgagctctt cggcgtctag aggtttgccg tgcagctcgc
     2461 cactcaggtg cacaacgccc gtgtggaact cggtggccgc ctgccccgtg gcgtgggtcg
     2521 agatggccca cgccgctgca tctccctcgc ggacgttgtc gagggcgaac atggcgccca
     2581 gccgccgcct gacgctttcg tctttgatcc gatcgtagaa aagccttgca tcatcccagt
     2641 ggtggtagtg gaaatcgaag gccatccctt ggtagttgac gctctgcttg tcctctccaa
     2701 cgtactggtc gttcgccttg cggtgggtca tgacccggat cttcatgccc gacatccccg
     2761 gcacgtcggt aaggcgcaac aggatgccgg tgatgttggt cgtgttgcac gagacgcaca
     2821 tgatgctctg atttggcccg tcgtgctctt cgtagtgttc tgccggctcc agaccatacg
     2881 tcgtgaaggc gtcgtggatg aagatcctgc cgtcagcgac cttctctggc gagttggacc
     2941 cgttgtagat ggtcggaacg ccgcgcccct cgtactcgtc gaagagcagc tctttgtagt
     3001 attcgctgat cctggtcttc tcgcccgtga ccgggtgttc gatgctcccc aatagcccgt
     3061 cgagaa
//
LOCUS       DASTUV010000464         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1692979, whole genome shotgun sequence.
ACCESSION   DASTUV010000464 DASTUV010000000
VERSION     DASTUV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692979"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFT03_09450"
     CDS             complement(<1..836)
                     /locus_tag="VFT03_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEU4848413.1"
                     /translation="MALRYPVDQRTLSKEDHVILKVLHLYYDHDLTQAEVGARMGFSR
                     PKVSKLIAEGKERGLVKIEIAEPADDLVPLEISLENRYGLREAVVVATSEDRATTELS
                     AGRAGAALLARVCTPGTVLGISWGISMRALADATPRRAFRCSKVVPLVGGMGKAHTQL
                     HSNQVCADLAEKLGAECLHLAAPAIARSSRSRAELVAMPGIGDTLAEGASCDVAAVGI
                     GGILPGSTMVEAGYFTPEEFLGLRERGIVGDVCCHFLDAEGRTRYPELSERIVGLTPE
                     ELR"
     gene            complement(840..2099)
                     /locus_tag="VFT03_09455"
     CDS             complement(840..2099)
                     /locus_tag="VFT03_09455"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4848414.1"
                     /translation="MSLRNKLEGLSQAGTPIRVGLVGAGQMGRGFVAQVSGIPGMEVV
                     AAADVDAERALTAFREAGSEPVEGLNGSPERPAVTTDAVELARSESVDVVVEATGVPE
                     VGARVAYEAIHNGKHVVMLNVETDVTVGAVLARMAKSAGVVYTGSAGDEPGAIMELYD
                     FARSLGFDVIVAGKGKNNPLDVSATPESLAEEAEQKRMNPKMLASFVDGTKTMVEMAA
                     VANATGFSPDTPGMHGPEETDPNRLGEVFSLADEGGLLSGYHTVDYVRGVAPGVFVVV
                     RSEEGPVRETLRYLGQGAGPNHVFYRPYHLTSLETPISVARAAVYGEPTIVPLPAPTA
                     EVVAVAKRDLKAGEKLAGIGSADYYGRVYRVEEATGMLPLGLASGARTTRPVARGEVI
                     ARSGVELAEESLVVALRRLQDTAAIEG"
     gene            complement(2123..2512)
                     /locus_tag="VFT03_09460"
     CDS             complement(2123..2512)
                     /locus_tag="VFT03_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEU4848415.1"
                     /translation="MTEPIYLTEVTEIGPEVAEFLEAGLLILFEAGAPPELAEIAVLH
                     QPISGPRENPPEAGDILAIGSREFRITAVGYKAWQNIQDLGHAVFKFSGHEEAELPGE
                     IHLEARGAEDLEDLVQPGARVEIRTGA"
     gene            complement(2521..>2916)
                     /locus_tag="VFT03_09465"
     CDS             complement(2521..>2916)
                     /locus_tag="VFT03_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848416.1"
                     /translation="GIITATGLGDLIANTLAPLANSLVGLLVLSVIVGLPFLSPVLGP
                     GAVIAQVIGVLIGDQIGRGAIDPQYALPALFAYNVQVGCDFVPVGLALGEAKPKTVEI
                     GVPAVLFSRQITGPMAVIIGWLLSFGLYS"
BASE COUNT          448 a          993 c          976 g          499 t
ORIGIN      
        1 cgcagttctt cgggtgtgag gccgacgatc ctctcggaga gctccgggta gcgcgtccgt
       61 ccctcggcgt cgaggaagtg gcaacacacg tcgcccacga tgccgcgttc cctgagcccc
      121 aggaactcct ccggcgtgaa gtacccggct tccaccatgg tcgagccggg gagtatccct
      181 ccgatcccaa cagcggcaac gtcgcaggac gcaccctccg caagggtgtc gccgatgccg
      241 ggcatggcca cgagctcggc ccggctgcgc gacgaccggg cgatcgccgg ggccgccagg
      301 tgcaggcatt cagctccgag cttctccgcg aggtcggcgc acacctggtt cgaatgcagc
      361 tgggtgtggg ccttgcccat gccgcccacg agcggcacca ccttgctgca gcggaaggcg
      421 cggcgcggcg tagcgtcggc aagcgcgcgc atcgagatgc cccaggagat acccaggacg
      481 gtcccggggg tacagactct cgcgaggagc gccgcacccg cgcgtcctgc cgagagctcc
      541 gtagtggcgc ggtcctcgct cgttgcgacc acgaccgcct cgcggagccc gtagcggttc
      601 tcgagggata tttcgagcgg tacgagatcg tcggcgggct cggcgatctc tatctttacc
      661 aggccccgct ccttcccctc ggctatgagc tttgagacct tcgggcgcga gaagcccatg
      721 cgggcgccta cctccgcctg ggtgagatcg tgatcgtagt agaggtggag gaccttcagg
      781 atcacatgat cctccttcga tagcgtgcgc tggtctacgg gatagcggag ggccaccgct
      841 tagccttcta tggctgccgt gtcctggagc cgccggagcg ccaccacgag actctcctcg
      901 gccagctcta ccccggatct cgctatgacc tcgcccctgg ctacgggcct ggtggtgcgg
      961 gcccccgacg ccagccccag cggcaacatg ccggtggctt cctcgaccct gtacacgcgt
     1021 ccgtagtagt cggcggagcc tatcccggcg agcttctctc cggccttcag gtcgcgcttg
     1081 gccacggcca cgacctcggc cgtgggagca gggaggggga ctatggttgg ctcgccgtag
     1141 actgcggcgc gcgcgacgga gatcggggtc tccaggctcg tgaggtggta gggtctgtag
     1201 aacacgtggt tgggtccggc gccctgtccc aggtaccgca gcgtctcgcg cacgggtccc
     1261 tcttcggagc gcacgacgac gaagacacca ggggccaccc cacgcacgta gtccacggtg
     1321 tgatagccgg agaggaggcc gccttcgtcg gcaaggctga agacctctcc caggcggttg
     1381 ggatcggtct cctcaggccc gtgcatcccg ggtgtgtcgg gggagaagcc tgtcgcgttg
     1441 gcgacggccg ccatctcgac catggtcttg gtgccatcaa cgaaggaggc gagcatcttg
     1501 gggttcatcc gcttctgctc cgcctcttct gcgagcgact cgggggtggc cgatacgtcg
     1561 agggggttgt tcttgccctt gccggctacg atgacgtcga agccaaggga gcgggcgaag
     1621 tcgtagagct ccatgatcgc gcccggctca tcgccggcgg agccggtgta gacgacgcct
     1681 gcgctctttg ccatccgcgc gaggaccgcc ccgaccgtga catcggtctc gacgttgagc
     1741 atgaccacgt gcttgccatt gtggatcgcc tcgtaggcca ccctggcgcc gacctcgggt
     1801 acgcctgtcg cctcgacgac gacgtccacg gattcggaac gcgcgagctc cacggcgtcc
     1861 gtggtgacgg cgggcctctc cgggctgccg ttcaggccct caacaggctc gctgcccgcc
     1921 tccctgaagg cggtgagggc gcgttcggca tccacatccg ctgcggccac gacctccatg
     1981 ccggggatac cggagacctg cgctacgaaa cctcggccca tctggcccgc gccgacgagc
     2041 cccacccgga tgggggtgcc ggcctgtgac aggccctcta gcttgttcct gagactcacg
     2101 aggctccctt ctacccgccg ctctaggcgc cggttcttat ctccacgcgg gcgccgggct
     2161 ggacgagatc ttccaggtcc tcggcgcccc gggcttcgag gtgtatctct cccggtagct
     2221 cggcctcctc gtggcccgag aacttgaaga cggcgtgccc gaggtcctgg atgttctgcc
     2281 aggccttgta gccaacggcc gtaatgcgga actccctgga tccgatggcg aggatgtcac
     2341 ccgcttctgg agggttctcc cgaggcccgg agatgggttg gtgcagcacg gctatctccg
     2401 ccagctccgg cggggctcct gcctcgaaaa ggatcaggag ccccgcctcc agaaactccg
     2461 cgacctccgg cccgatctct gtgacttctg tcaggtagat aggttcggtc acgacggcac
     2521 ctacgagtag agcccgaacg agagcagcca gcctatgatg accgccatgg gaccggtgat
     2581 ctggcggctg aagaggaccg ccgggacccc tatctccacg gtcttgggct tcgcctcgcc
     2641 gagcgccagc ccgacgggta cgaagtcgca gccgacctgc acgttgtagg cgaagagcgc
     2701 cggcagcgcg tactgcgggt ctatggcgcc gcgcccgatc tggtccccga tcaggacccc
     2761 tatgacctgc gctatcaccg caccgggtcc gaggacaggt gagaggaacg gcagaccgac
     2821 gatcacggag aggaccagaa gcccgaccag gctgttcgcg agcggcgcga gggtgttggc
     2881 gatcaggtcg cctaggcccg tggcggtgat gatgcc
//
LOCUS       DASTUV010000465         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1372034, whole genome shotgun sequence.
ACCESSION   DASTUV010000465 DASTUV010000000
VERSION     DASTUV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372034"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFT03_09470"
     CDS             complement(<1..402)
                     /locus_tag="VFT03_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU4848417.1"
                     /translation="MAAILRSALSMSVATTLSRITGYARTMVQAAVLGTGAVANAYTL
                     SNALPTQLYELFIGGLLSSILVPLLVERLTRYGEDDARRLTNALLTAILPFLAVLVLI
                     ACVFAGPLVNLVTAWSPAEGFSREEAREATSL"
     gene            670..1734
                     /locus_tag="VFT03_09475"
     CDS             670..1734
                     /locus_tag="VFT03_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEU4848418.1"
                     /translation="MNAYPIKLTYHVRSYYFGDRLIPDLLGKRDAPGGVVAETWEISD
                     YEETTGRVTNGEYAGKTLHGLVTDFPEELVGEGWSGPHFPLLEKFLDASHMLPVHLHP
                     DDETARKKHGEPHGKTEAWHILWAAEGATILAGIKDGLSREELFEAFKAQDYDAVMPR
                     HPVRAGDTVYVPGGIIHTFGPDTLIFEVQQTSDLGQFVMPEDLYGNRLSEEEWDANIN
                     ATLDELKRDYLPRPNPGLVVQDGPNRRVMCCAGPHFALERWTLTGPYTEPAHPERCLT
                     LSNVGDPVRIEYGGGTERLERAESCILPAAIGEVLLIPEGESSLISCYVPDLERDVIS
                     PLRASGYSDEEIRGLGEVNI"
     gene            1763..2713
                     /locus_tag="VFT03_09480"
     CDS             1763..2713
                     /locus_tag="VFT03_09480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEU4848419.1"
                     /translation="MGKLVTLGEVVSDIYRGDELSDVELGFIARPGGAPANVAVAASR
                     LGAVASFVGSVGEDLFGDFILRALESEGVDTSGVGRQPQPTRTSLAFVEIDESGDRAF
                     TFYRTSPAADELLSPEDVTENLVSGASFVNFGSIPLIREPVRSAPHRIVDLADELDVP
                     VAFDVNFREHLWESPDAAREAIDPLFDRSRIVKLSGDEIPPLLGTDDSGEAADLLLDR
                     GAALVFISLGPDGAFYASKEFRGSVQAFEVEAVDATGAGDAFLAAILAHLSGETWNEE
                     RVREAALRGSAAGALACTGYGAMGPLPTKDELERFMAREG"
     gene            complement(2718..>3919)
                     /locus_tag="VFT03_09485"
     CDS             complement(2718..>3919)
                     /locus_tag="VFT03_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU4848420.1"
                     /translation="RRREKEGRRGAGDGLRRRSAVLACARGSLPGGLYNAHLDVARVR
                     DIVDAIERIAPASLSEDWDNCGLQVGTAGAEASRVLVALTPVDEVFEEAERQGADFLL
                     FHHPLLFHPSKSVDTGSYPGDLVARAVRKDLAVFAAHTSYDAAPEGVSVALAEALGLR
                     GTLEVLSPRGALRKLVIFVPEDNVDAVADVLASEGAGVIGEYTRCTFRTPGTGTFLGG
                     EATDPYLGEKGRLEKVEEIRLEIVVPAHAAGRATAAAVAAHPYEEAALDLYPIEGHPA
                     GCGYGRAGRLPDPMSPEELEEHVASSLGFPARLVADPEPGRRVEQVAVLGGSGGSFVP
                     EAAASGAQAYVTGDLDYHDALLAHSLGLTAIDAGHAATELPSLAPLAKRLAQMVDVPV
                     EVSRVRR"
BASE COUNT          677 a         1298 c         1319 g          625 t
ORIGIN      
        1 gagcgaggtc gcctctcgcg cctcctcgcg tgagaacccc tcggccgggc tccaggctgt
       61 taccaggttg acgagcggtc cggcgaagac gcaggcgatg aggaccagga cggcgaggaa
      121 cggtagtatc gcggtgagca gtgcgttggt gagccggcgg gcgtcgtcct ccccgtaccg
      181 ggtgaggcgt tctacgagca ggggaaccaa gatggaggag agcagccctc ctatgaagag
      241 ctcgtagagc tgggtcggca gggcgttcga gagggtgtag gcgttcgcca cggcgcctgt
      301 gccgagaact gcggcctgga ccatagtcct ggcgtagccg gtgatgcgcg agagcgtggt
      361 cgccaccgac atggagagcg cggatctcag gatggcggcc ataccggaaa atgttacatc
      421 ccccgctccg agggtgccgc agcccggaaa agatgcacca attcctacga taatcgatcc
      481 ctgcaagact tcgagagagt ccgctccccc caaaccaaaa gctcaattgc tgacaagccg
      541 ccgaccgaag ggaggcggcg tgctgagaag cggaagccga agagcgtagc gtgctgaatc
      601 cttacccgac gtcgctgccg aaggtagaat atccgcactc gggaaagagg ggagagtgac
      661 tctaggggga tgaacgcgta cccgataaag ctgacctacc acgtgcgctc ctactacttc
      721 ggggaccgtc tgatccccga cctgctcggc aagcgggacg ccccgggagg cgtcgtcgcc
      781 gagacctggg agataagcga ctacgaggag actaccggac gcgtcaccaa cggcgagtac
      841 gccggcaaga cgctccacgg cctcgtaacc gatttccccg aagagctcgt gggagaaggc
      901 tggagtggtc ctcacttccc gctcctcgag aagttcctcg acgcctcgca catgctcccc
      961 gtccacctcc atcccgacga cgagacagcc aggaaaaagc atggcgagcc ccacggcaag
     1021 accgaggcgt ggcacatcct ctgggcagcc gaaggcgcga cgatcctggc cgggatcaag
     1081 gacggcctgt cgcgcgagga gctcttcgag gcgttcaagg cccaagacta cgacgccgtg
     1141 atgccgcgcc atcccgtgcg ggctggggac acggtgtacg tgccgggtgg gatcatccac
     1201 accttcggac ctgacaccct gatcttcgag gtccagcaga cttccgacct cggccagttc
     1261 gtgatgcccg aggacctcta cggcaaccgc ctgagcgagg aggagtggga cgccaacata
     1321 aacgccaccc tcgacgagct aaagagggac tatctgcccc gccccaaccc cggcctcgta
     1381 gtgcaagacg gcccgaaccg ccgcgtaatg tgctgcgccg gcccgcactt cgccctcgag
     1441 cgctggacac tcaccgggcc ttacacggag cctgcccacc ccgagcgctg tcttaccctc
     1501 tcgaacgtgg gggacccggt gcggatcgag tacgggggag ggacggagag actcgagcgg
     1561 gccgagtctt gcatattgcc tgcggccata ggagaggtgt tgctgatccc cgagggtgag
     1621 tcgagcctga tctcctgcta cgtgccggac ctggagcggg atgtcatctc tccgctgaga
     1681 gcatccggct actccgacga ggagatccgg ggcctcggcg aggtgaacat ctagccgccg
     1741 cgagaccgaa cgggagggag ctttgggcaa gttagtcacg ctaggggagg tcgtctcgga
     1801 catctaccgg ggggatgagc tctcggacgt cgagctgggg ttcatagccc gccctggcgg
     1861 cgcgccggcg aacgtggcgg tcgccgcctc caggctcggc gccgtggcct cgttcgtggg
     1921 cagcgtgggg gaggacctct tcggtgactt catcctgcgg gcgttggagt ccgagggggt
     1981 agacacctcg ggtgtcggcc gccagccgca gcccacgcgc acctcgctgg ccttcgtgga
     2041 gatcgacgag tccggcgacc gcgcgttcac cttctaccgc accagccccg ccgccgacga
     2101 gttgctctcg ccggaagacg ttaccgagaa cctggtttcg ggggcttcgt tcgtcaactt
     2161 cggcagcatc cccctgatac gcgagcccgt ccgctccgcg ccgcaccgta tcgtggacct
     2221 cgcggatgag ctcgacgttc ccgttgcctt cgacgtgaac ttccgcgagc acctctggga
     2281 gagcccggac gccgcgaggg aggccatcga cccgctcttc gaccgctccc gcattgtcaa
     2341 gctgagcggc gacgagatac ctccactgct cgggacggat gactccgggg aggcggcgga
     2401 tctgctcctg gaccgcggcg ccgctctggt cttcatcagt ctcgggccgg acggagcctt
     2461 ctacgcctcg aaggagttca gaggcagcgt ccaggccttc gaggtcgagg ccgtggacgc
     2521 gaccggtgcg ggggacgcct tcctggcggc gatcctggcg cacctctctg gcgaaacctg
     2581 gaacgaggaa agggtgcgcg aggcggcgct gcgcggatcg gccgccggag ctctggcctg
     2641 caccggctac ggggccatgg gcccgttgcc cacaaaggac gagctggaac gtttcatggc
     2701 tcgcgaaggc tgagccgcta gcgccgcacc cggctgacct cgacagggac gtctaccatc
     2761 tgcgcgagcc ttttcgcgag cggtgcgagc gagggtaact ctgtggcggc gtggccggcg
     2821 tctatagcgg tcaggccgag ggagtgcgcg agcaacgcgt cgtggtagtc gaggtcgccc
     2881 gtgacgtaag cctgggcgcc cgaggctgcg gcttccggga cgaaggagcc gccggacccc
     2941 ccgaggaccg cgacctgctc caccctccgg cctggttccg gatccgctac caggcgtgcg
     3001 gggaaaccga gggagctcgc gacgtgctcc tctaactcct cgggcgacat cggatccggc
     3061 agcctgccgg cccggccgta accgcagccc gccgggtggc cttcgatggg gtacaggtcc
     3121 agggccgctt cttcgtaggg gtgggccgcg acagccgcgg ccgtggcgcg gccggcggcg
     3181 tgggcgggga ccacgatctc gagccggatc tcctcaacct tctccagccg gcccttctcc
     3241 cccaggtagg ggtccgtagc ctcgcctccc agaaatgtgc ccgttcccgg cgtccggaag
     3301 gtgcatctcg tgtactctcc gatgacgccg gctccttcgg aggccaggac gtcggccacg
     3361 gcgtccacgt tatcctcggg aacgaagatc accagcttcc gtaatgcacc cctgggagat
     3421 agcacctcca gggtgccgcg cagcccgagc gcctctgcca gggcgaccga gacgccttcg
     3481 ggggcggcgt cgtagctcgt gtgggccgcg aagacggcga ggtctttgcg tacagcgcgc
     3541 gccaccaggt cccctggata agaaccggtg tcgacgcttt tcgagggatg gaatagaaga
     3601 gggtggtgga agagcaggaa gtctgccccc tgccgctcgg cttcctcgaa gacctcgtcc
     3661 acgggcgtga gcgcgaccag aacccggctc gcctcagccc ctgccgttcc tacctggagc
     3721 ccgcagttgt cccagtcttc ggagagggat gcaggagcaa tccgctcaat ggcatccact
     3781 atgtcccgta cgcgcgccac gtccaggtga gcattgtaca atcccccggg tagtgagcct
     3841 cgcgcgcaag ccagcacagc tgatctccgc cgcaacccgt ctcccgctcc tcgccgtccc
     3901 tctttttctc gtcgtcggg
//
LOCUS       DASTUV010000466         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_263150, whole genome shotgun sequence.
ACCESSION   DASTUV010000466 DASTUV010000000
VERSION     DASTUV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263150"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFT03_09490"
     CDS             complement(<1..452)
                     /locus_tag="VFT03_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4848421.1"
                     /translation="MGSGRTDRHFTVAVFVVWEGKVLLHWHRKLAMWLPPGGHIEDGE
                     LPDEAALREVREETGLWAELVGERREDVADPVQLHRPAGVQLENIGPGHQHIDLIYFA
                     KPSGSTEIQEAFSGDRVGWYGPEDWEGMRINAEVRGWCERALTSVGAL"
     gene            complement(546..815)
                     /locus_tag="VFT03_09495"
     CDS             complement(546..815)
                     /locus_tag="VFT03_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848422.1"
                     /translation="MAREKRESYTSTTSTLEYCSFDWMKRHATKSVPLGGVFWDAGAQ
                     VFINKGVNGRWAETLTPEDVAEYEARAVQELGPECARWLATGEEL"
     gene            1080..1736
                     /locus_tag="VFT03_09500"
     CDS             1080..1736
                     /locus_tag="VFT03_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848423.1"
                     /translation="MSSLPEGAFRRFDESPDEEFYLLPRFVTHIDDRAISAVTQLYRE
                     FFPPGGEILDLMSSWISHLPPEVRYRRVIGLGMNEAELQRNERLDSYVVQNLNTNPHL
                     PFGGAEFDGCGICVSIDYLTRPVEVLREVGRVLKVGSPTVITFSNRCFPTKAIAFWHQ
                     LDDRGRTRLVERYLREADNFEDVRSLDRSPRRLFTDPLFAVIGRSTGPFAEGRESPRA
                     "
     gene            1870..2850
                     /locus_tag="VFT03_09505"
     CDS             1870..2850
                     /locus_tag="VFT03_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4848424.1"
                     /translation="MKVPGIQMLKKAERKGNEAEVPMTEDLGLVDFFKLTFKEVDEDH
                     VSAFAGNLTYKAIFAIFPFFTFLLSLLGLFNATDLVNTMIEGLSGVVPESARQFIEEQ
                     LLSITKSQAQDTFTFAAIISILLALWGVSGAFRSIMEAMNVMYEVEEDRPFWKKYGIS
                     IFISLAVAALMLTAFGIVVFGRSIGGAVAEAIGLGAVFQVVWTIVQWPIVACIVLFTF
                     AVIYYFAPAAKQRFRWISPGAVLAFVFWLLFSLAFSYYAGNFSSYNETYGSLAGVIIF
                     MLYIYYSALIMLVGAEMNQVIEWHIPGGKNEGEKVPEAERQPNVRPTRQK"
BASE COUNT          525 a          944 c          851 g          569 t
ORIGIN      
        1 tacagcgcgc cgaccgacgt cagcgcccgc tcgcaccagc ccctgacctc tgcgttgatg
       61 cgcatgccct cccagtcttc gggaccgtac cagccgaccc tgtctccgga gaaggcttcc
      121 tggatctccg ttgagcccga aggctttgcg aagtagatca ggtctatgtg ctggtgtccg
      181 ggcccgatgt tttcgagctg caccccggcc ggtcggtgca gctgcacggg gtcggcgacg
      241 tcctcgcgcc tttcccccac gagctcggcc cacagccccg tctcctccct gacctcgcgc
      301 agcgcggcct catcgggcag ctcgccatct tcgatgtgcc cgcccggcgg gagccacatc
      361 gccagcttgc ggtgccagtg caggaggacc ttcccctccc atacgacgaa gacagcgacc
      421 gtgaagtgcc tgtcggtacg cccggatccc ataagtcacc agggtacaac aggagataca
      481 ggcggcacct gtcctctttc agcgggagga aacgaatccg gggccggtga ttcttcggcc
      541 gggacctaga gctcctcacc cgttgcgagc catcgcgcgc attcggggcc aagctcctga
      601 acggcccgcg cctcgtactc cgctacgtcc tccggcgtga gggtctccgc ccaacggccg
      661 ttcacccctt tgttgataaa cacctgggct ccggcgtccc agaagacacc cccgaggggg
      721 acactcttgg tggcgtgccg cttcatccag tcaaaggagc agtactcgag agtcgaagtg
      781 gtggatgtgt agctctcgcg tttttctcgg gccaacaatg gtttcggtca tcgtccttgc
      841 tcctcctctg taccgctcgc cgcgtgctct acattacgat ccccagtttg cggctcggcg
      901 tggagccgtc ccagccacac tagccccaca gttgccccac gctcctccaa cgctttgatc
      961 agcgcacgcc tcgtgcggcg gtgacacgaa gccgggtcca aggtacaacg taacagagga
     1021 ggcgcgcact tgcggccaag atcgccgagt agtgcgctag aggccaggag aggagaccga
     1081 tgtcgtcatt accggaaggg gccttcaggc gctttgacga gtcacccgac gaggagttct
     1141 acctgctgcc gcgcttcgtc acccacatag acgacagggc catatcagct gtcacccaac
     1201 tgtacaggga attcttcccg cctggaggcg agatcctgga cctgatgagc agctggatct
     1261 cgcacctccc gcccgaagtc cggtaccggc gcgtgatcgg tctcgggatg aacgaggcgg
     1321 agttgcagcg gaacgagagg ctcgactcat acgtcgtcca gaacctgaac acgaacccgc
     1381 atctgccttt cggtggcgcc gagttcgacg ggtgtgggat ctgcgtctcc atagactacc
     1441 tgacacgacc ggtggaagtg ctgcgcgaag tcgggcgcgt cctgaaggtc ggttctccta
     1501 cggttattac cttctcgaat cgctgttttc ctaccaaggc catcgcattc tggcaccagt
     1561 tggatgaccg gggtcgcacg cgactcgtcg agcgctacct ccgcgaggcg gacaacttcg
     1621 aggacgttcg cagcctggac cgcagcccgc gccgcctgtt caccgatccg ctcttcgcgg
     1681 taatcggcag aagcacgggg ccatttgcgg aggggcgaga atcgccccga gcctgatctg
     1741 ccttacgatg tccgaaggtg ggcgctctcc tggcctctcg acgtatctgc gcgcaagagg
     1801 ttagaccgac catatctgcc gggtactaat agggcagacc agaaggcgta cttcagaagg
     1861 gatgccagca tgaaggtacc cggcatacag atgctgaaaa aggcagaaag gaaagggaac
     1921 gaggccgaag tccccatgac cgaggatctc ggcctcgtag acttcttcaa gcttaccttt
     1981 aaggaggtgg atgaggacca cgtctcggcc ttcgcgggca acctaaccta caaggcgatc
     2041 ttcgccatct tcccgttctt caccttcctg ctgtcgctgc tgggtctctt caatgccacc
     2101 gacctggtca acaccatgat cgagggcctc agtggcgtgg tccctgagtc ggccaggcag
     2161 tttatcgagg aacaactcct gagcatcacc aagagccagg cgcaggacac cttcaccttc
     2221 gcggccataa tctcgatcct gctggccctc tggggtgtct ccggggcgtt ccggtcgatc
     2281 atggaagcga tgaacgtcat gtacgaggtg gaggaggacc ggccgttctg gaagaagtac
     2341 gggatctcca tcttcatctc tctcgctgtc gccgctctca tgctcaccgc cttcggcata
     2401 gttgtcttcg gccgctcgat cggcggcgct gtcgccgagg ccatcgggct gggggcggtc
     2461 ttccaggtgg tgtggaccat agtgcagtgg ccgatcgtcg catgcatcgt gctgttcacc
     2521 ttcgcggtga tctactattt cgccccggcg gccaagcaga ggtttcgctg gatcagcccc
     2581 ggcgccgtcc tcgccttcgt cttctggctc ctgttctcgc tcgcgttctc gtactacgcg
     2641 ggcaacttca gctcttacaa cgagacttac gggtcgctgg ccggcgtgat catcttcatg
     2701 ctctacatct actactcggc gctcatcatg ctcgtaggcg ccgagatgaa ccaggtgatc
     2761 gagtggcaca tccccggcgg caagaacgag ggcgagaagg tccccgaggc cgaaaggcag
     2821 ccgaacgtcc gtccgacccg ccagaaatag tagaactccc cggtcacccg gagaccccgt
     2881 gaagactcg
//
LOCUS       DASTUV010000467         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1228522, whole genome shotgun sequence.
ACCESSION   DASTUV010000467 DASTUV010000000
VERSION     DASTUV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228522"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFT03_09510"
     CDS             <1..257
                     /locus_tag="VFT03_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848425.1"
                     /translation="SARQAEFLHHEVPGINIPKHVREKLAALPPEDAPRYGVEVTQNL
                     LSRAQSLVGGAYIMPPASAPDLAGDVMEAIDKASEKRAGS"
     gene            324..>2502
                     /gene="metH"
                     /locus_tag="VFT03_09515"
     CDS             324..>2502
                     /gene="metH"
                     /locus_tag="VFT03_09515"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU4848426.1"
                     /translation="MKQARISEFRALLGQRILILDCAMGTMIQSYGLSERDYRGERFE
                     DHSNELKGNNDLLCLTQPRIIRDIHEATLEAGADIVETNTFNANKISQSDYGTEDLAY
                     ELNREAARLAREAADAYTEQRPEKPRFVAGALGPTNRTASLSPDVNNPGMRNVTFDEL
                     AAAYKGAARGLVDGGADLLLVETIFDTLNAKAAIFGIKEYLEEENLEVPIMISGTITD
                     ASGRTLSGQVTEAFWNSIRHAEPISVGLNCALGSKELRQYVEELSRIAGVPVSAYPNA
                     GLPNEFGEYDETPEIMAAEVGDWARNGWLNIVGGCCGTTPEHVKAIAEAVSDYPPRVV
                     SEVPRLTRLSGLEACNVGPDSLFLNVGERTNVTGSRRFKDLILNGDYETALEVARQQV
                     ENGAQMIDVNMDEGMLDGEEAMVRFLNLVASEPDISRVPVMIDSSRWSIIEAGLKCVQ
                     GKAVVNSISLKEGEDEFIRQAKLLRRYGAAVIVMAFDEEGQADTKERKVEICKRAYEI
                     LTEKVHFPPEDIIFDPNVFAIATGIEEHDNYGVDFIEAVREVSATLPYARTSGGISNV
                     SFSFRGNDVVREAIHAVFLYHAIDAGLTMGIVNAGQLAVYDALDEELREAVEDVVLNR
                     RSDATERLLALAEEYRGRDETTKEDETLEWRTWPVERRLEHALVKGIAEYVEEDTEEA
                     RQNYDRPIEVIEGPLMDCMDVVGDLFGSGRMFLPQVVKSARVMK"
BASE COUNT          523 a          780 c          833 g          366 t
ORIGIN      
        1 ggagcgcccg ccaggcggag ttcctccacc acgaggttcc gggcataaac atcccgaaac
       61 acgtcaggga gaagctcgca gcccttccgc cagaagacgc cccaaggtac ggtgtcgagg
      121 tgacgcagaa cctcctctcg agggcgcaga gcctcgtcgg cggcgcctac atcatgccgc
      181 ccgcgagcgc cccggatctg gccggcgatg tcatggaagc gatagacaag gcttcagaga
      241 aaagggcagg aagctgatgc ccgatacctg tagcctcaga cccttgtgag agcattatgg
      301 tcctagaacg aaccgcagaa gaagtgaagc aggctcgaat ctccgagttc cgggcgctgc
      361 tcgggcagcg catcctcatt ctggactgcg cgatggggac catgatccag agctacgggc
      421 tctcggagag agactacagg ggagagcggt tcgaggacca ttccaatgag ctcaagggca
      481 acaacgacct gctctgcctg acgcaacccc ggatcatccg ggacatccac gaggccaccc
      541 tcgaggcagg cgcggacata gtcgagacca acaccttcaa cgcgaacaag atctcacagt
      601 cggactacgg gacagaagac ctcgcctacg agctcaaccg cgaggccgcg cggctcgccc
      661 gcgaggcggc ggacgcctac acggagcaga ggccggagaa gcctcgcttc gtcgcgggcg
      721 cccttggccc caccaaccgc acggcttccc tctcccccga cgtgaacaac ccgggtatga
      781 gaaacgtcac ctttgacgag ctcgccgcgg cctacaaagg ggcggcgcgg gggctggttg
      841 acggcggcgc ggacctgctg ctcgtcgaga ccatcttcga cacgctcaac gcgaaggccg
      901 cgatcttcgg gataaaggaa tatctcgaag aggagaacct ggaggtcccg atcatgatct
      961 ccggcacgat cacggacgcg agcgggcgca cgctctcggg ccaggtgacg gaggccttct
     1021 ggaactccat tcgccacgcc gagcccataa gcgtcggcct gaactgcgcc cttgggtcga
     1081 aggagctgag gcagtacgta gaggagctct cgcgcatagc cggcgtcccc gtgagcgctt
     1141 accccaacgc gggcctcccg aacgagttcg gcgagtacga cgagaccccc gagataatgg
     1201 ccgccgaggt tggggactgg gccagaaacg gctggctcaa catagtcggc ggctgctgcg
     1261 gcaccacgcc ggagcacgtg aaggccatag ccgaagccgt atccgactac ccgccgcgcg
     1321 tggtgtccga ggtgccccgt cttacacgcc tcagcgggct ggaggcgtgc aacgtcgggc
     1381 cagactccct gttcctcaac gtcggcgagc gcaccaacgt cacgggctcg cggcggttca
     1441 aggacctcat cctgaacggc gactacgaga cggcgctaga ggtagcccgc cagcaggtcg
     1501 agaacggcgc ccagatgata gacgtcaaca tggacgaagg gatgctcgac ggcgaggagg
     1561 cgatggtccg cttcttgaac ctcgtcgcaa gcgagccgga catctcgcgc gtgcccgtga
     1621 tgatcgactc ctcgaggtgg tcgatcatcg aggccggcct gaagtgcgtg cagggcaagg
     1681 cggtggtgaa ctccataagc ctcaaggagg gcgaggacga gttcatccgc caggcgaagc
     1741 tgctcaggag gtacggggcc gccgtgatcg tgatggcctt cgacgaggag ggccaggcgg
     1801 acacgaagga gcggaaggtc gagatctgca agcgcgctta cgagatcctg accgagaagg
     1861 tccacttccc gcccgaggac atcatattcg accccaacgt ctttgccatc gccaccggta
     1921 tcgaggagca cgacaactac ggcgtggact tcatcgaggc ggtgcgcgag gtctcggcga
     1981 cgttgccgta cgcgcgtacg tcgggcggta tctcgaacgt ctcgttctcc ttccgcggca
     2041 acgacgtggt gcgggaggcg atccacgccg tcttcctcta ccacgccata gacgccggcc
     2101 tcaccatggg catagtgaac gctggccagc tcgccgtcta cgacgcgttg gacgaggagc
     2161 tgcgcgaggc cgtagaagac gtggtcctga accggagaag cgacgcgacc gagcgcttgc
     2221 tggccctcgc cgaggagtac cgcggcaggg acgagacgac gaaggaagac gagacgctcg
     2281 agtggcggac gtggcccgta gagagacggc tcgagcacgc cctcgtcaag ggcatcgccg
     2341 agtacgtcga ggaggacacg gaagaggcgc gtcagaacta cgaccggccc atagaggtga
     2401 tcgagggtcc gctgatggac tgtatggacg tggtcgggga cctcttcggg tctggcagga
     2461 tgttcctgcc gcaggtagtc aagagcgccc gcgtgatgaa ga
//
LOCUS       DASTUV010000468         4441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_923551, whole genome shotgun sequence.
ACCESSION   DASTUV010000468 DASTUV010000000
VERSION     DASTUV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923551"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..632
                     /locus_tag="VFT03_09520"
     CDS             123..632
                     /locus_tag="VFT03_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4848427.1"
                     /translation="MAKRAPIQEILEDLLRATDASRTTLRLDLSDQDAGLDAVVAEAL
                     APGVRSIRDDTSIRNLRGVSTVRFLEEQRRVLVQNDCLADDLAPPSELIEVYGVKAQM
                     LAPIVRVDRLEGLISVHYAPGPRDWSSEDVAVLKEAVERVRRALDATVQANREIDDGE
                     LEESQDPDP"
     gene            595..1608
                     /locus_tag="VFT03_09525"
     CDS             595..1608
                     /locus_tag="VFT03_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848428.1"
                     /translation="MESWRNRKTLILSRTDMMGLLEPGEYNSCVEHAYRMHGEGRYYM
                     EPKGHIVLEKYPGEWEVMPSYIEEPEAAACKWVSIREENREKFDLPTVFSILIYTHPE
                     TGFPLAICDGSYHTVMRTGAAAAVSAKHLARQDSKVLAIVGAGNMAEGTLATCNEVFE
                     WEDVRVWSRSHATLDEFVKNEQPKYDRFEIRASSNLEEVVRGADVVVTVTPARGPIVE
                     NAWISEGTHIAAVGADKEGDQELDPEILGRARIFVDDIRQCRTDGEINVPLRKGMISE
                     DDIAGEIGQVVAGKLEGRASDTDITVFDSTGFALQDSATVPLEYERALAAGVGIEKKM
                     IST"
     gene            complement(1605..2372)
                     /locus_tag="VFT03_09530"
     CDS             complement(1605..2372)
                     /locus_tag="VFT03_09530"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4848429.1"
                     /translation="MERSDTPTTLITGAGIGIGAATARAFANAGSRVIVTDVLDEEGR
                     SVASGIESGGGEAEYQHLDVTDTEEVDSVMSGVQDRYGPLHTVVANAGIAHRIPLTEL
                     TDEKWDHTHDVDLKGVMRVCRAAAPAMREAGRGSMIAVSSIMGVAYGWDEHVQYSAAK
                     AGVVGFVRGLAVELSRDGVRVNGIAPGYIRTAQALSEEHSLGPEGLEQAAEFIPMGRV
                     GEPDDIADVIVFLASEAARYITGQVLVVDGGLLVGRY"
     gene            complement(2412..3158)
                     /locus_tag="VFT03_09535"
     CDS             complement(2412..3158)
                     /locus_tag="VFT03_09535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEU4848430.1"
                     /translation="MTSERSRAVLVTGAGSGIGRAIAEKLAKDGERVVVNDLKGETAD
                     EVVARIKESGGEAAAAPGDVSDPESVQRIVEAAREAYGPPEILVNNAGFLQQKRFVDL
                     TVEDFDRMIAVHLRGTFLCTSAVLPDMLSRGSGIIVNVASQLGQIGGIELCHYSAAKA
                     GIIGLTKSLAREVSNQGVRVNAVAPGPINTELVLGLSEEWRDAKSAELPLGRFGEPHE
                     VAETVAFLVSDGAALYVGQTLGPNSGDVML"
     gene            complement(3253..>4441)
                     /locus_tag="VFT03_09540"
     CDS             complement(3253..>4441)
                     /locus_tag="VFT03_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848431.1"
                     /translation="GWATATSTTIATAVAIGTFFVAILVGPMIDYLGRKKSLIITTAG
                     TAVSSGLTGLTPATFAAPYLTVIRSISGLGYSEQAVNTTYLNELYGNRKSRGFIYAFI
                     QGGWPIGVLLAAAFTALLLGSVGWRGVFVLATIPALIVAGLGMRLKESPRFLAMQRVR
                     ELEKEGRHEEAVEFGRHFDIDVHHVQETTLQQIFEPDIRKHTIFLSLAFLTNWIGIQV
                     FAVLGTTILTDGKGVSFGSALTVLIVSNAAAYIGYLCHGFVGDRIGRRLTIAGGWVIS
                     GLAYTLMLFGPDSDGFVLTMYTIGLFFIIGPYAALLFFMGESFPTRVRGTGASFVNAM
                     GPIGAIAGGALLSGFLVLTGENVVLSAFLSGGLATIISGLLMLGTRDVKDPREAEAVE
                     GSA"
BASE COUNT          829 a         1447 c         1415 g          750 t
ORIGIN      
        1 aagtacgccg acgtggtgtc catcgaggat gccctcgcct atatcgagag cgtgtcacga
       61 aaggccggcg cctcggtgta atgagaccgg ccacggcctc ataaggacaa gcgggaggcg
      121 tcatggccaa gcgggcgccg attcaagaga tactggagga ccttctcagg gcgacggacg
      181 ccagccggac gacgctgcgc ctggacctct cggatcagga cgccgggctc gatgcggtcg
      241 tggcggaagc ccttgcgccc ggcgtccgct cgatcaggga cgatacttcg atacgcaacc
      301 tgcgcggcgt ctcgacggtc aggttcctag aagagcagag aagggtgctc gtgcaaaacg
      361 attgcctggc ggacgatctg gctccgcctt cggagctgat agaggtctac ggcgtaaagg
      421 cgcagatgct cgcgccgatc gtgcgcgtag accgtctgga ggggctgatc tcggtccact
      481 atgctcccgg cccccgcgac tggagctctg aagacgttgc ggttctcaag gaggcggtcg
      541 agcgcgtgcg gcgagcacta gacgcaacgg tacaagccaa cagggagata gacgatggag
      601 agttggagga atcgcaagac cctgatcctt agccgcaccg acatgatggg cctgctggag
      661 cccggcgagt acaactcctg cgtcgagcac gcctaccgga tgcacggcga gggccgctac
      721 tacatggagc cgaagggcca catcgtgctg gagaagtacc ctggcgagtg ggaggtgatg
      781 ccctcctaca tcgaggagcc cgaggccgcc gcctgcaagt gggtctccat ccgcgaggag
      841 aaccgcgaga agttcgacct tccgaccgtc ttctccatcc tcatctacac ccacccggag
      901 acggggttcc ctctggcgat ctgcgacggc agttaccaca ccgtgatgcg caccggggcc
      961 gcggcggcgg tctccgcgaa gcacctggcc cgccaggaca gcaaggtact agccatagtc
     1021 ggcgcgggga acatggcgga aggcaccctc gcaacctgta acgaggtatt cgagtgggag
     1081 gacgtgcgcg tctggagccg ctcgcacgcc acgctcgacg agttcgtaaa aaacgagcaa
     1141 ccgaagtacg accgcttcga gatccgggcg tcttcgaacc tcgaagaggt agtccgcggg
     1201 gcggacgtcg tcgtcaccgt tactccggcc aggggaccta tcgtcgagaa cgcgtggatc
     1261 tccgagggca cccacatcgc cgccgtggga gcggacaagg agggtgatca ggagctggac
     1321 cccgagatac tcgggcgggc ccgcatcttc gtcgacgaca tccgccagtg ccggactgac
     1381 ggggagatca acgtcccact ccgaaaaggg atgatctccg aggacgatat agcgggcgag
     1441 atcggccagg tcgtcgccgg gaagctcgaa ggacgcgcct ccgacaccga catcaccgtc
     1501 ttcgactcga cgggcttcgc ccttcaggac tcggcgacgg tgccgctgga gtacgagcgt
     1561 gcgctcgccg ccggagtcgg catcgagaag aagatgatct cgacttagta gcggcctaca
     1621 agtaacccgc cgtccaccac gagcacctgt cctgtgatat agcgggcggc ctccgaggcg
     1681 aggaacacga tcacgtcggc gatgtcatcg ggctccccca ccctgcccat cgggatgaac
     1741 tcagcggcct gttcgagccc ctctggaccc agggagtgct cctcggagag cgcctgggca
     1801 gtccgtatgt agcccggcgc gatgccgttg acgcgcaccc catcacggga gagctcgacg
     1861 gccagaccgc gcacgaatcc caccacgccg gccttggcgg cagaatactg gacgtgctcg
     1921 tcccaaccgt aggcgacgcc catgatcgag gagacggcga tcatgcttcc tcgtcctgcc
     1981 tctctcatgg ccggggcagc ggcccggcat acccgcatga cccctttcaa gtccacgtcg
     2041 tgcgtgtgat cccacttctc gtcggtaagc tcggtgagag gtatccggtg ggctatcccg
     2101 gcgttggcca cgaccgtgtg tagcggaccg taccgatcct gaacgccgga catcacagag
     2161 tcaacctcct ctgtgtccgt gacgtcaagg tgttggtact cggcctcgcc gcccccggat
     2221 tcgatgccgc tggcgaccga tctcccctcc tcatccagta cgtccgtcac gatgacccgg
     2281 ctgccggcgt tcgcgaacgc gcgggccgtc gccgcgccaa tacctatgcc agcgccggtg
     2341 atcagtgtgg taggcgtatc agagcgctcc atgcgagttc ggtctcctcg aacagggtta
     2401 ccttcggtgg ttcacagcat cacgtcgccc gagttggggc cgagcgtctg gccgacgtag
     2461 agcgcagccc catcagagac caggaaggct accgtctcgg ctacctcgtg tggctctccg
     2521 aagcgtccca gaggaagctc ggctgacttg gcgtcgcgcc actcttccga tagccccagg
     2581 acgagctcgg tgttgatggg tccgggcgcc accgcgttca ccctgacgcc ctggttcgag
     2641 acctcgcggg caagcgactt ggtcaggcct atgatgccgg ccttggcggc gctgtagtgg
     2701 cacagctcga taccgccgat ctgacccagc tgggaggcga cgttgactat gatgccgctg
     2761 ccccgtgaaa gcatgtcggg caatacggcg ctggtgcaca ggaacgtacc gcgcaggtgc
     2821 acggcgatca tcctgtcgaa gtcttcgacc gtaaggtcga cgaaccgctt ctgctgcagg
     2881 aacccggcgt tgttcaccag gatctcggga ggaccgtaag cctcgcgcgc cgcctccact
     2941 attcgctgta cggactccgg atcggagacg tcgcctggcg cggccgctgc ctctcccccc
     3001 gactccttga tccgggcgac gacctcgtcg gcggtctcac ctttcaggtc gttgacgacc
     3061 actcgctcgc cgtccttcgc gagcttctcg gcgatggcgc ggccgatccc gcttcccgcc
     3121 cctgtaacca ggacggctcg ggagcgttca gaggtcatcg gcgccctcct tccattggct
     3181 aaaaggggcg ggcccgatca ggcccgcccc tttgcttctt ccttcagcgt acgaacgcgt
     3241 gccctcacgc ggctatgcgg acccttccac cgcctcggct tcgcgcgggt ccttcacgtc
     3301 gcgggtaccc agcatgagga gccctgagat gatcgtcgcg agcccaccac tcaagaacgc
     3361 ggagagaacc acgttctctc cggtcaacac gaggaagccg ctgagcaacg cccctccagc
     3421 gatcgccccg atcgggccca tggcgttgac gaacgaagcc ccagtgccgc gtaccctggt
     3481 cgggaacgac tcgcccatga agaacagcag agccgcgtag ggcccgatta tgaaaaacag
     3541 accgatcgtg tacatggtca gcacgaagcc gtctgaatcc ggcccgaaca gcatcaacgt
     3601 gtaggcgagg ccggatatca cccagccacc ggcgatggtc aggcgccggc cgatgcggtc
     3661 gcccacgaac ccgtggcaga gatagccgat gtaggcggcc gcgttggaga ctatgaggac
     3721 ggtcagcgcg ctcccgaacg aaaccccttt gccatccgtg aggatggtgg tcccgaggac
     3781 ggcgaagacc tgtatgccga tccagttcgt caggaaggcg agggagagga agatcgtgtg
     3841 cttcctaatg tcgggctcga agatctgctg cagggtggtc tcttgtacgt gatgaacgtc
     3901 tatgtcgaag tggcggccga actcgacagc ctcctcgtgc cggccctcct tctcgagctc
     3961 ccggacccgc tgcatggcca ggaacctcgg cgactctttg agcctcatgc cgaggccggc
     4021 gactatgagc gctggtatgg tggcgagcac gaacacaccc cgccatccca cggacccgag
     4081 cagcagcgcc gtgaaggccg ccgcgagcag cactcctata ggccacccgc cctgtatgaa
     4141 ggcgtatatg aacccccggc tcttccggtt accgtagagc tcgttgaggt aggtcgtatt
     4201 caccgcctgc tcagagtatc cgagaccgga gatggatcga atgaccgtca ggtaaggtgc
     4261 cgcaaacgtc gcgggagtta gccccgtgag gccggagctt acggccgtgc cggcggtggt
     4321 gattatcagc gacttcttgc ggcccaggta gtcgatcatc ggccctacca ggatggctac
     4381 gaagaaagtc ccgatcgcga ccgcggtcgc gatcgtagtg ctggtagcgg tcgcccagcc
     4441 g
//
LOCUS       DASTUV010000469         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1049308, whole genome shotgun sequence.
ACCESSION   DASTUV010000469 DASTUV010000000
VERSION     DASTUV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049308"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2068
                     /locus_tag="VFT03_09545"
     CDS             <1..>2068
                     /locus_tag="VFT03_09545"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEU4848432.1"
                     /translation="DGSILEDLLALHDPDRPTVGVIFYRTHLLGGNTAFVDALVDALQ
                     RAGANAFPTYCYSLRPSADARVPALQLLEGRVDCLVTTVLASGGSNAADAQSGDGSEG
                     WLEWEVPALEELGVPVVQGICTTSSREAWLRSDAGLSPLDTAWQVAIPEFDGRIVSVP
                     FSFKERVEADSPVGASLSVYEADPERTQRLAGLAARFARLGRTPNGRKKVAVLLSNYP
                     TRHSRVGNAVGLDTPASAVLLLRAMKDAGYAVDGAPEEGDALIHALIDAGGHDLEFLT
                     EEQLGGATGRLDAVRYAEWFARLPEELRARVEEHWGKPPGELYVDGGEIVVSGLAFGN
                     VFIGIQPPRGFGENPIAIYHDPDLPPTHHYLAVYWWIIEEFGADAMVHLGKHGTLEWL
                     PGKSLGLSSACSPDAALRDVPLFYPFVVNDPGEGTQAKRRAHAVVVDHLIPPMTRAET
                     YDDLARLEQLLDEYYQVETLDPSKLPAIRARIWETLRDAELHRDLGVEEQPEEFGDFL
                     NHVDGYLCEIKDLPIRGGLHILGKPPEGEPMRHLLAAILRLGAGDVPGLRRAVGAAFG
                     LDERFLSENGGARMVAPEQLVARFPGAAATASDLVDRLEEATQTLLLAMEGRGWEAEA
                     AEEVCEEVLGIVDAGVALSLRFAATEVVVRLARTPEEMGNLLGGLSGGYVPAGPSGSP
                     TRGLVNV"
BASE COUNT          314 a          698 c          732 g          324 t
ORIGIN      
        1 gacggctcga tcctggagga cctcctcgcg ctgcacgacc cggatcgccc gaccgtcggc
       61 gtgatcttct accggaccca ccttttggga ggaaacacag ccttcgtgga cgcgctcgtg
      121 gatgcgctgc agagagccgg cgcgaacgct tttcccacct actgctactc gctgcgtccc
      181 agtgcggacg cgagggtccc ggcgctacag ctgctggagg gacgggtgga ctgcctcgtt
      241 acaaccgtgc tcgcgagcgg gggctcgaac gccgcggacg cacagagcgg ggacggatcg
      301 gagggctggc tcgagtggga ggttccggcg ctcgaggagc taggggtgcc ggtggtgcag
      361 ggaatctgta cgacctcttc gcgcgaggcg tggctccgct ccgacgccgg gctctcgccg
      421 ctcgacaccg cctggcaggt ggccataccg gagttcgacg gcaggatcgt ctccgtcccc
      481 ttctccttca aggagcgggt ggaggcagat tctcccgttg gagcatctct gagcgtctac
      541 gaggcagacc ccgaacgcac gcaacgcctg gcgggcctcg cggcccgctt cgctcgcctc
      601 gggcgcaccc cgaacgggcg gaagaaggtg gctgttctgc tctccaacta tccgaccagg
      661 cactcgcggg tgggcaacgc cgtcggtctc gacaccccgg cgagcgccgt cctgttgctc
      721 cgtgcgatga aggacgcagg atacgcggtg gacggcgctc ccgaggaggg ggacgccctg
      781 atccacgcgc tcatcgacgc cggggggcac gaccttgagt tcctcacgga ggagcagctc
      841 gggggagcca cagggcggct ggacgcagtc cgctacgcgg aatggttcgc gcgcctcccg
      901 gaggagctga gggccagggt cgaggagcac tgggggaagc cgccaggcga gctctacgtg
      961 gacggcgggg agatcgtggt gtcgggactc gccttcggga acgtcttcat cggcatccag
     1021 cctcccaggg ggttcgggga gaaccccatc gccatctacc acgaccccga cctcccgccg
     1081 acgcaccact acctcgccgt ctactggtgg atcatcgagg agttcggcgc cgacgccatg
     1141 gtacacctcg gcaaacacgg aacgttggag tggctcccgg gcaagtccct ggggctctcc
     1201 tccgcctgct cccctgacgc cgccttgcgt gacgtgccgc tgttctatcc gttcgtcgtc
     1261 aacgacccgg gcgaggggac gcaggccaag cggcgggcgc acgcggtcgt ggtcgaccac
     1321 ctcatccccc cgatgacccg agccgagacc tacgacgacc tagcgaggtt ggagcagttg
     1381 ctcgacgagt actaccaggt cgagacgctc gacccgtcga agcttccggc catacgagcc
     1441 aggatctggg agacgttgcg cgacgccgag ctgcacaggg acctcggcgt agaggagcag
     1501 cccgaggagt tcggcgactt tttgaaccac gtggacggct acctgtgcga gatcaaagac
     1561 ctcccgatcc ggggcggcct gcatattctg ggcaagcccc ccgaaggcga gcccatgcgg
     1621 cacctcctcg ccgcgatcct gcgcctcggc gccggcgatg tccccggcct gcgccgtgcc
     1681 gtcggagcag ccttcggcct cgacgagcgc ttcctctccg agaacggcgg cgccaggatg
     1741 gtggccccgg agcagctcgt cgcccgcttc cccggcgcgg ccgcaacggc ttcggacctg
     1801 gtcgaccgtc tggaggaagc gacgcagacc ctcctcctcg cgatggaggg gcgcggctgg
     1861 gaagcggagg cggctgaaga ggtctgcgag gaggttctgg gtatcgttga cgcgggcgtc
     1921 gcgctctcgt tgcgcttcgc cgccacggag gtcgtggtgc gcctcgctcg cacgccggag
     1981 gagatgggga acctgctggg cggtctttcg ggcggctacg tgccggctgg accgtccggg
     2041 tcgccgacgc gcgggctggt gaacgtgc
//
LOCUS       DASTUV010000470         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_802457, whole genome shotgun sequence.
ACCESSION   DASTUV010000470 DASTUV010000000
VERSION     DASTUV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802457"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..266
                     /locus_tag="VFT03_09550"
     CDS             12..266
                     /locus_tag="VFT03_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848433.1"
                     /translation="MNIFFDVQGTLLTEDSDPRPHVREFFLKLSDMGHHVYLWSTAGD
                     GYAAHTARVLGVDDVVRGSFGKRSVPEDITVDFVVDDQPS"
     gene            complement(341..1321)
                     /locus_tag="VFT03_09555"
     CDS             complement(341..1321)
                     /locus_tag="VFT03_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU4848434.1"
                     /translation="MADARDSIAHLILNPAAGGAAASMGRLSEAARERGIRVSVLEPG
                     EDARHAALAAAEDGAQVLGVAGGDGSVAAVAGVAVERGLPLVVVPTGTLNHFASDLGL
                     DLVRPLGALDAFFAGDELRVDVGRINGRIFINNVSLGVYAEMLGDPGYRRDKLRVAQT
                     KLQAAVFDLELRRALRISPPDEAPLEGVVAVVVSNNPYELAPLSALGQRHRLDTATLQ
                     VSVLDVSTLDELERLLAGTLLGAVEFRPGLRHWTVDRLEMGDPGATMRAGVDGEPITF
                     EAPLRLSVDPGALRVLVPEGLPASRQMPPLEAGWHAARTLRRWLRPPPDS"
     gene            complement(1314..2132)
                     /locus_tag="VFT03_09560"
     CDS             complement(1314..2132)
                     /locus_tag="VFT03_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848435.1"
                     /translation="MLLPLAVRRSKLYQAIIGRLLRITIELVGGVEGVYPAQEIPVRE
                     LLVRKTAGNVVELGSIVAVGWSPLWLLAGASDLVGGTKVYLRALVAELRDAGVLAPDA
                     DVASFDELLTTLEDASGVLADAVDVPPLNVPSMRASWQELRRQAEDLPDAAALETIFA
                     ELQLAARQEDRSMLEISSIVALGAVHAGVKLGNVHIFDYYRRALRTIVDEGLLSFLRR
                     ISSPYLTRAGSHFDPRSSTYSERLLRRWADRRSPVGRQKTPQTEASDEENAASG"
     gene            complement(2494..2778)
                     /locus_tag="VFT03_09565"
     CDS             complement(2494..2778)
                     /locus_tag="VFT03_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848436.1"
                     /translation="MRKRAITRKKRAASEGQAAFVRVGIGRVATGAAAFGALAVGAGA
                     IGALAIGALAIRALAVKRGRIQRLNIEELEVGRLHVRELVVEQEQRASAT"
BASE COUNT          545 a         1059 c          940 g          554 t
ORIGIN      
        1 tgtaggatga gatgaacatc ttctttgatg tccagggcac gctactcacc gaggacagcg
       61 atcctcgacc ccacgtcagg gagttcttcc tcaagctctc cgatatgggc caccatgttt
      121 atctgtggtc cacggctggc gatgggtacg cagcccacac cgcacgggtc cttggggtag
      181 acgacgtggt gagaggttcc ttcggaaagc ggtcggtgcc ggaggacata accgttgatt
      241 tcgtcgttga cgatcagccc tcataggtag aggaacacgg tggcttcttc gtgaaagctt
      301 acgatgggtg cgagcccgac ctcctctact ggcacagaat ctaggagtcc gggggcgggc
      361 gcaaccagcg gcgaagcgtc cgggcggcgt gccagccggc ctctagtggc ggcatctgac
      421 ggctcgcggg aagcccctcc ggcacgagaa cgcgcagcgc accggggtcc accgacaagc
      481 gcaacggcgc ctcgaaggtg atcggctcgc cgtctacccc ggcccgcatt gtcgcaccag
      541 gatcgcccat ctccagacgg tcgaccgtcc agtggcgcag gcccgggcgg aactcgacgg
      601 cgccgagcaa cgtccccgcg agcagccgtt cgagctcatc gagcgtgctg acgtcgagca
      661 cggagacttg tagcgtcgct gtgtccaggc ggtggcgttg cccgagcgcg gacagcggcg
      721 cgagctcgta tgggttgttc gagacgacca cggcgacgac accctcgagc ggcgcctcgt
      781 cgggcgggga gatccggagc gcccgacgga gctcgaggtc gaagacggcg gcctggagct
      841 tcgtttgcgc cacgcgcagc ttgtcgcgcc tgtagccagg atctcccagc atctccgcgt
      901 agacgccgag agagacgttg ttgatgaaga ttcgcccgtt gatccgtccg acgtccacgc
      961 gcagctcgtc gccggcgaag aacgcgtcga gggctccaag cgggcgtacg aggtcaagcc
     1021 cgaggtcgct tgcgaagtgg ttgagcgtgc ccgtgggcac cacgacgagg ggcaacccgc
     1081 gttccacggc caccccggcc accgccgcaa ccgacccgtc gccgccggcc accccgagca
     1141 cctgagcgcc gtcctccgct gccgcgaggg cggcgtgcct cgcgtcctcc ccaggctcca
     1201 gcacgctcac ccggatcccg cgctcgcgag ccgcttcgct cagcctaccc atggaggccg
     1261 ccgcgccgcc ggcggcgggg ttgaggatca ggtgggcgat ggagtccctg gcatcagcca
     1321 ctggcggcgt tttcctcgtc cgatgcttcg gtctgcgggg tcttctggcg ccccacaggc
     1381 gagcgccgat ccgcccagcg ccgtagcagg cgttcggagt aggtgctcga ccggggatcg
     1441 aaatgggacc cagcccgcgt caggtagggg ctgctgattc gccgcagaaa ggagagcaga
     1501 ccttcatcca cgatcgtccg caacgcgcgg cggtagtagt cgaagatgtg aacgtttccc
     1561 agcttcacgc ccgcatgcac cgccccgagc gcaactatgg aagagatctc cagcatcgag
     1621 cgatcctctt gccgtgccgc taactgcagc tcggcgaaga tcgtctcgag agcggcggcg
     1681 tcaggcagat cctccgcctg tcgccgtagt tcttgccacg aggcgcgcat gctggggacg
     1741 ttcaaagggg gcacgtctac cgcatcggcc agcacgccgg aggcgtcctc tagcgtagtc
     1801 aacagctcat cgaaagacgc cacgtccgcg tcgggcgcca gtacgcctgc atcccgtaac
     1861 tccgcgacca gcgcccgcag gtacaccttc gtacccccga ccaggtcgga agcccccgcc
     1921 aggagccaga gcggtgacca gccgaccgca acgatgctgc cgagctcgac cacattaccc
     1981 gccgtcttgc gtaccagcaa ctcccgtaca ggtatttcct gggccgggta gacgccctcc
     2041 acgccgccta ccagctcgat cgtaatgcgc agcagccgtc cgattatcgc ctggtagagc
     2101 ttggatctcc ggactgcgag cggtaggagc acctcacctg tctcgtatac caacccaccc
     2161 gccagcgcag ctcccgctcg agcggttcgc tcgggtaggg ataccaggta cgaccgcgac
     2221 agtacagtcc ggggttctgt tttgttcttc atatcgttca ctgtccgttt actgtagttt
     2281 ttcgaccctt tcgggccagc gaatagtctc gccgttgtac gcgagtataa gccggcccgc
     2341 gagctagcac atggggcggc ggacccactt cggctagtcc cgtggaccga ttagtgatcg
     2401 cagcgaggct gaatcccgct ttctactctg gtactgctat tgacctcgca agcagcaccc
     2461 tactacccgc gcgcagaata aaatggccgc gtgtcaggta gcgcttgccc tctgctcctg
     2521 ctccacgacc agctccctca cgtgcagcct gcccacctct agctcctcta tgttgaggcg
     2581 ctggatcctg cccctcttga cggcgagagc ccggatcgcc agggcgccga tcgccagagc
     2641 cccgatcgcg cccgccccta ctgccagagc cccgaacgcc gcagccccgg tcgccacccg
     2701 tccgatcccc actcgcacga aggccgcctg accctcggag gcagcacgct tcttccgcgt
     2761 tatcgcccgc tttctcatga tcctccttcc ttgtccatca tctccctgat ctctcgtggc
     2821 tggacaacaa tccccacgtc cggcctgatt ctccgacaag atgttgcttc ctggcggcag
     2881 gtagttggtc ggttagctgt ccccttacgt aacgttcacc gggcacgtaa cgactgcatc
     2941 ttactcttct gtgaaggaat gtatgtgcca cagggcacca agttatctga ggaacttccg
     3001 agcgcttggg tgaacaaggg tcttcgtgaa gccagaagca tccatgtcga tcacgaaacc
     3061 tcgtcaacga acgagacttc gtagaggtgc ggctcaaa
//
LOCUS       DASTUV010000471         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1193417, whole genome shotgun sequence.
ACCESSION   DASTUV010000471 DASTUV010000000
VERSION     DASTUV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193417"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..1028)
                     /locus_tag="VFT03_09570"
     CDS             complement(426..1028)
                     /locus_tag="VFT03_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU4848437.1"
                     /translation="MKLRFSQLLCVVILGGALLVISDGYAQTKTGLASYYGPELAGQP
                     TASGEPFNPYGFTAAHRTLPLGTKLIVSYGGHSVEVTVNDRGPYSGGRELDLSQGAAD
                     YLGLTYAGVDYVDYTVVGGYGAGGYSGGGGSTYTATDGLSQAPAAQFSSNGQSGTYVV
                     QAGDTLYGIAAELGTTVDDLVVTNGIEDPNLLYVGETLRY"
     gene            complement(1341..>2312)
                     /locus_tag="VFT03_09575"
     CDS             complement(1341..>2312)
                     /locus_tag="VFT03_09575"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEU4848438.1"
                     /translation="KSGSREVFREAGIPLPDGFEHLRDQEDIAEAIVELKRRDPGLRR
                     VAVKLDEGFSGEGNAVFNYDGAPEGMDLDRWVRDELPGRIKFEAKDETWERYCHKFEE
                     MSGIVECWVEGEEVRSPSVQCRINPLGQSAVVSTHDQVLGGPTGQIFLGSSFPADAEY
                     SRDITEAGQKVGRVLNERGALGRFAIDFISVRRGDGWEHAAIEINLRKGGTTHPYLIL
                     RFLTDGAYDANDGLYCTPTAQPCYYYASDNLQSLAYKGLTPDDLIDIAVNNGLHFDGA
                     SQQGVVFHLIGALSQYGKLGTVCIGDSHESAREFYRETVSVLDREAR"
BASE COUNT          416 a          762 c          689 g          445 t
ORIGIN      
        1 cgatctccta cctaacctac cgcctgttct tcgccggctg aggaaggatg aagccctggc
       61 gcggcacatc aaggattttt ggacagggtc tcccacagca gctagagccc tgatcatcct
      121 ggctatcgcg gcctgcatag ccgcaacggt atacctgttt ctttaaccct gacgttaatc
      181 ttgatgtcgc caggggtacc ctgaacctcc gcgtcgagcg ccaggagcct cgatacggaa
      241 gaggatgtga ggagatcccg cgtggacgac atcgcgctcg tattcgaact cggccttctc
      301 catcgcgctt cgaacccacc cccaggcacc tcgcagcctt acagcctccg ggccagtcta
      361 tccgatcgag gaaccggaga accgcgcgtg ggcgctgttg ttcgcgccca cgcgcatggc
      421 cttacctagt acctgagcgt ctcgccaaca taaaggaggt tgggatcttc tatgccgtta
      481 gtcactacaa ggtcatcgac cgtggtaccc agctcggcgg ctatgccgta gagggtgtct
      541 cccgcttgca caacgtaagt tccgctctga ccgttggatg agaactgcgc ggccggtgcc
      601 tgggacaagc cgtccgtcgc agtgtaggtt gagccgcccc cgccgctata gcctccggca
      661 ccgtagccac ccacgacggt gtagtccacg tagtccacgc cggcgtaggt gaggccgagg
      721 taatccgccg ccccctgcga gagatccagc tctctgccgc ccgagtaagg tccgcggtcg
      781 ttgacggtca cctcgacgga gtgcccaccg tagctgacga ttagcttggt accgagcggc
      841 agcgtcctgt gtgccgccgt gaagccgtaa gggttgaaag gctcgccgct ggcggttggt
      901 tgccctgcca gctcggggcc atagtacgag gccagacccg tctttgtctg cgcgtacccg
      961 tccgaaatga cgagtaaggc gccgcccaga atcaccacgc acaacagttg cgagaatcta
     1021 agcttcacgc atccccctcc agtctgcgtt agccattaac caaaaacaaa atccacaaac
     1081 taaggccgag agtattgggg ctacaaaacg gcatttcaag gcctttttcg ccgttatcac
     1141 ggcgtgatca cggctctagc accgtccaca cggggggcct tcctcgcctt ttccccatgg
     1201 tttcgcgaat ccggccggac gaagcatggt ggaggccggg aggcgctctg ctgcaaaata
     1261 tttcgacgat aatccggcgg aggcccgtag cggagccggg cgagggcgcg agagggtggt
     1321 accggcctta cgagcctgtg ctaccgggct tcgcggtcga ggacggagac ggtctcgcgg
     1381 tagaactcgc gggcgctctc gtggctgtcc ccgatgcata cggtgccgag cttgccgtac
     1441 tgggagaggg ctccgatcag gtggaacacc acgccctgct gggaggcgcc gtcgaagtgg
     1501 agaccgttgt tcacggcgat gtctatgagg tcgtcggggg tgagaccctt gtaggccagg
     1561 ctctggaggt tgtccgaggc atagtagtag cagggctgcg ccgtgggcgt gcagtacagc
     1621 ccgtcgttag cgtcgtaggc cccgtcggtc aggaaacgaa ggatcaggta cggatgggtc
     1681 gtcccaccct tgcgcaggtt gatctctatg gccgcgtgct cccagccatc ccctcgcctg
     1741 acggagatga agtctatggc gaaacggccc agagccccgc gctcgttgag cacccttccc
     1801 accttctgcc ccgcctccgt aatgtcccgg ctgtactctg cgtcggcggg gaaggagctg
     1861 cccaggaaga tctgacccgt cggtcccccc agcacctggt cgtgagtcga gacgacggcc
     1921 gactgaccca gcgggtttat gcggcactgc accgacgggg agcgcacctc ctccccctcg
     1981 acccagcact cgactatgcc gctcatctcc tcgaacttgt ggcagtagcg ctcccacgtc
     2041 tcatccttcg cttcaaactt gattcgcccc ggcagctcat ctctcaccca gcggtcgagg
     2101 tccatgcctt caggagcgcc gtcgtagttg aagacagcgt tgccctcacc ggagaaacct
     2161 tcgtccagct tgaccgcgac gcgccgcaac ccggggtcac gccgcttcag ctcgactata
     2221 gcctccgcga tgtcctcctg gtcgcgcagg tgctcgaacc cgtccggcag cggaatgccc
     2281 gcctcccgga atacctcccg gctcccgctc tt
//
LOCUS       DASTUV010000472         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1086065, whole genome shotgun sequence.
ACCESSION   DASTUV010000472 DASTUV010000000
VERSION     DASTUV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086065"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFT03_09580"
     CDS             complement(<1..481)
                     /locus_tag="VFT03_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4848439.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGALFGKLSRAITVAAREGGGDPE
                     MNAALGLAVSKAKDANMPHDNIQRAIDKGTGAGSDAETFERITYEGYAPGGVAVLVEV
                     LTDNRNRAASDVRYLFSKNGGKLGTSGSVAYLFERKGVILVPRGSVDEDELMELAIEA
                     "
     gene            complement(468..1076)
                     /gene="pdxT"
                     /locus_tag="VFT03_09585"
     CDS             complement(468..1076)
                     /gene="pdxT"
                     /locus_tag="VFT03_09585"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU4848440.1"
                     /translation="MDGNDERKVAPKIGVLALQGDFREHVEVLRKLGVEPVEVREVGD
                     LQGLAGIIVPGGESTAIGNLMVSSGLLDGVRSFFYKGGPVWGTCAGMVLAASATTGPR
                     QPLLGLMNALVERNGFGRQVYSFEKDLDVEGFDEPFTGVFIRSPYFEDVGPGVEVLSE
                     IDDKVVAARGENILVTAFHPELTDDTRFHEYFLQEVCGIERS"
     gene            complement(1066..1962)
                     /gene="pdxS"
                     /locus_tag="VFT03_09590"
     CDS             complement(1066..1962)
                     /gene="pdxS"
                     /locus_tag="VFT03_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU4848441.1"
                     /translation="MADNGQARGTFRVKSGMAQMLKGGVIMDVVNAEQARIAEEAGAV
                     AVMALERVPADIRAQGGVARMSDPEKIVEIQEAVTIPVMAKARIGHFVEAQVLEALEV
                     DYIDESEVLTPADEANHVDKWAFTVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGN
                     VVEAVRHMRSIVGGIRRLGTLGSEELMTEAKNLGAPYDLVRWVAENGKLPVVLFTAGG
                     IATPADAALMMQLGADGVFVGSGIFKSGDPASRARAIVKATTHFRDPKVVAEASKGLG
                     EAMVGREMGDLSQGERLATRGW"
BASE COUNT          386 a          832 c          669 g          485 t
ORIGIN      
        1 ctgcctcgat cgccagctcc atgagctcgt cctcgtcaac ggaacctctg gggaccagga
       61 ttacgccctt gcgctcgaag aggtaggcca cggagccgct cgtccccagc ttgccgccgt
      121 tcttggagaa gaggtagcgg acgtctgagg ccgcccgatt cctgttgtcc gtgaggacct
      181 cgacgagcac ggctactccg ccgggggcgt agccctcgta ggtgatccgc tcgaacgtct
      241 cggcgtcgga gccagcgccg gtgcccttgt ctatggcgcg ctggatgttg tcgtgcggca
      301 tgttggcgtc tttggccttc gagaccgcga gccccagcgc cgcattcatc tctgggtctc
      361 ccccgccctc gcgggccgct accgttatgg ctctgctgag cttgccgaag agcgcgcccc
      421 gcttggcgtc ggcggctcct ttcttgcgct tgatcgtcga ccatttgcta tgaccgctca
      481 attccgcata cctcctgtaa aaaatactcg tgaaacctgg tgtcgtctgt cagctcgggg
      541 tgaaacgccg tcaccagtat gttctcaccc cgcgcggcga cgaccttgtc gtctatctcg
      601 gagaggacct cgacccccgg gccgacgtcc tcgaagtacg gcgaccggat gaagaccccg
      661 gtgaacggct cgtcgaagcc ctccacgtcg aggtcttttt cgaaggagta gacctgccgc
      721 ccgaagccgt tgcgctccac gagcgcgttc atcagcccga gcagcggctg gcgcggaccc
      781 gtcgtcgccg aggccgctag caccatcccg gcgcacgtgc cccacacggg cccgcccttg
      841 tagaagaagc ttcgcacgcc gtcgaggagt ccggaggaca ccattaggtt ccctatggcc
      901 gttgactcgc ctccaggcac tattatacca gcgaggcctt gcaggtcccc cacctcgcgc
      961 acctcgacgg gctcgacgcc gagcttcctg aggacctcga cgtgctccct gaaatcgccc
     1021 tgcaaggcca ggaccccgat cttcggggct accttcctct catcgttacc atccacgcgt
     1081 cgcgagccgc tctccctggg agaggtcgcc catctccctg ccaaccatcg cctcgccgag
     1141 acccttgctc gcctccgcta ccaccttcgg gtcgcggaag tgggtcgtgg ccttcacgat
     1201 cgcccgcgcc ctgctcgcgg ggtcgccgct cttgaagata ccgctcccga cgaagacccc
     1261 gtccgccccg agctgcatca tcagcgccgc gtcggccggc gtggcgatgc ctccggcggt
     1321 aaacagcacc actggcagct tcccgttctc tgcgacccac cgcacgaggt cgtaaggggc
     1381 cccgaggttc ttggcctcgg tcatcagctc ctcggagcct agagtaccca ggcgcctaat
     1441 tccaccgaca atggagcgca tgtgccggac ggcctcgacg acgttccctg tcccggcctc
     1501 gcctttggac cggatcatgg ccgcgccctc gcctatgcgc ctgagcgcct ctcccaggtt
     1561 cgtcgcgccg cagacgaagg gcactgtgaa ggcccacttg tcgacgtggt tagcctcgtc
     1621 ggcgggcgta aggacttccg actcgtctat gtagtcgacc tcgagggcct cgagcacctg
     1681 ggcctcgacg aagtggccga tgcgggcttt cgccataacg ggtatggtga ctgcctcctg
     1741 gatctccact atcttttccg ggtcgctcat gcgcgccacg ccgccctgcg cccttatgtc
     1801 ggccggcacc ctttcgaggg ccatgacggc cacggccccc gcctcctcgg cgattctagc
     1861 ctgctccgcg ttgaccacgt ccatgatgac cccgcccttg agcatctggg ccatcccgct
     1921 cttcaccctg aaggtgcctc ttgcctgacc gttatctgcc atatgttctc ccggcgctgc
     1981 ttactagaat tcgtaagcgt tatcttacta ccggcgcccc cacgcgccaa acgtcacgcg
     2041 ggtaggaggc ttgcctagag cttcctgcgc tctttgggaa actcctttta gttccgatta
     2101 ctcttacaat aaaggattct ggtccggact ctaataatat agcggctgag gttgccactc
     2161 gtcatcgccg ggcgtcagaa cgcaacattt gcctttggcg aagcctttgg cgaacagatg
     2221 gaggttcgac catgacagct gaatacggtc accatacaga gcttactgag tccgatctta
     2281 ccgaccttag gtacgcgagg tccttactcg agaaccccag cctgaccgca cagatcagtg
     2341 atgtcgttgg gacgccgata gaaaaagtac tt
//
LOCUS       DASTUV010000473         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1300737, whole genome shotgun sequence.
ACCESSION   DASTUV010000473 DASTUV010000000
VERSION     DASTUV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300737"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(952..1101)
                     /locus_tag="VFT03_09595"
     CDS             complement(952..1101)
                     /locus_tag="VFT03_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848442.1"
                     /translation="MSETETELGESEAGICNICGQTLASQAEISTHLTDAHPDDELSD
                     SAEDL"
     gene            complement(1193..1510)
                     /locus_tag="VFT03_09600"
     CDS             complement(1193..1510)
                     /locus_tag="VFT03_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4848443.1"
                     /translation="MERESTPLHVETDSDGTILLSGELDMATVKLLEETIAEVMRPGQ
                     PIVIDMAALNYIDSSGLGLLVWIHQQSGERVVICNPSRQVRRVLEIMDGRAKPEAWVI
                     QAG"
     gene            1758..2084
                     /locus_tag="VFT03_09605"
     CDS             1758..2084
                     /locus_tag="VFT03_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848444.1"
                     /translation="MGFREERVARNEVAAREINEAIEGTDTRPASSFIHVLCECGVED
                     CERVLAITRPVYERVRNDPRQFVITPPHLIAEVEDVVLESAGFLIVAKRRGTPEDVAL
                     RTDPRR"
     gene            2246..2593
                     /locus_tag="VFT03_09610"
     CDS             2246..2593
                     /locus_tag="VFT03_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848445.1"
                     /translation="MLLTKDERLVLQNLASGRSIEEIAATLNWPLQAVRWTCTHPLIT
                     WVLDELDASTTPADSPPRKRTGTKDAAFAVGYAIGLVSGPIRFLVMVVVGGAIVLGFA
                     HALGFFWRILPDG"
BASE COUNT          528 a          893 c          885 g          578 t
ORIGIN      
        1 tcgcgtctca ccttggagca gaggttggcc gaatgggagg cggcctggcg ttgtcgaccg
       61 agctcttgtc ggctcgacga gaccgaacgc aatggcgtct gactacaacg cgccaagacg
      121 tcgagtcacg tgttgtcatc acatgtgctg gactgcagtc cgaccgtgtg gcgcggcgga
      181 ttgcctcccg caccaagtat caaatcgttc ccttcgaggg aaagtacgtc cgcctctcct
      241 ctcgaggagg cggcctcgtt cgagggctgg tctatccggt tcccgttccc ggcctcccat
      301 ttctgggcgt gcatttcact cgacgagttg atgacgaggt ttgggtcggc cccaacgctg
      361 ttctggctcg aggcagggaa ggttacggag gacattggaa cctccgggac ttgcgcgaat
      421 cggtcgcgta tccagggtcc tggagactcc tcagagcgca atggcgcata gcactgaggg
      481 aggagtggca gtacaagtca acccgcgcct ttcttcatga atgccggcgg tacgtccccg
      541 atctggaagg ggccgacttg ctgagtgagt tcagcggtat ccgggctcag gccgttggac
      601 gagacgggtc cctgatcgat gacttcaagc tcttgaaacg gccgggattg gttcatgtcg
      661 cgaacgcgcc ttccccggct gcgacaacct ccctagcgat tggcgagatc ttggcgagag
      721 aggtgctgca agaacttcga caacccggtt cgagtgctca ggtctggggc tgacgggaag
      781 ggcttcggag tcagtgggcc gagcctgcgt cgggagtagc gggcgcgaag gtcgaagttg
      841 gggccgggtc tccggctaac ctccttggcc cggccctctt cgacacatcg atcggatcaa
      901 agaagatctc ggggactgcc ccggtgggcg atctcacctt gggacgaggc tctataagtc
      961 ctccgccgaa tcagagagtt catcgtcggg atgggcatcc gttaggtgtg tcgagatctc
     1021 cgcttgcgag gcgagggtct gcccgcaaat gttgcagatg ccggcctcgc tttcgcccaa
     1081 ctcggtttcc gtctcgctca ttgcgcactc ccttcgctct cttgaagctt ccctcccatc
     1141 cgtgcctcaa aacggtcctc gttggaccaa ggccaaagcc tgagcggact gatcagcccg
     1201 cctggatcac ccacgcttcg ggtttggccc gcccgtccat gatctccaag acgcgacgga
     1261 cctggcgtga cgggttgcat atgacgaccc gctctcccga ctgctgatgg atccagacca
     1321 gcaagccaag cccgctgctg tcgatgtagt tgagcgcggc catgtcgatg acgatcggct
     1381 gtccaggcct catgacctcg gcaatcgtct cttcgagcag cttcaccgtg gccatgtcca
     1441 actcgccact gagcaggatc gtcccgtcgg aatcagtctc cacatggaga ggagttgatt
     1501 ccctttccat atgcggctcc tttgagcagg tgtctgactg cgagattttg agcagcgctt
     1561 gaaccgcatt tccagactgg tttgtgcacc gaagtacgat tagagtgctg gcggttttgt
     1621 atcccgattt ccaaggatgg gaaccggcga ggaagccact tggtgccggc cgctggggcg
     1681 ccagcgtaaa accatcacct ccccgcagca tcccgacgag caccgcccct agcgtccgga
     1741 acccatatgc tcgcaaggtg ggctttcgcg aggaacgggt cgctcggaac gaggtggctg
     1801 cgcgggagat caacgaggcg attgagggga ccgacacccg ccccgccagc tccttcatcc
     1861 acgtgttgtg cgaatgcggc gtggaggact gcgaacgcgt cctggccatc actaggcctg
     1921 tgtacgagcg cgttcgaaac gatcctcgcc agttcgtcat cacgccgccg catctcatcg
     1981 ctgaagtcga ggatgtagtg ctcgagagcg ccggattcct catagtcgcg aagcgcagag
     2041 gaacgcccga ggacgtggcc ctccgaacgg accccaggcg ctagagatac agccacgccc
     2101 cagggcgtct agaaggcctg gaaccgtcgt tcgagttcat ctggcggcac gcgtcacgct
     2161 atctcgtggc tgacaacaca gtcatttgca gatgaaaggt gcacagagta atactccgtt
     2221 cggccgagag gggtgcatca tagcgatgct actcacaaag gatgaacgac tagttctgca
     2281 gaatctcgcc tcgggcagaa gcattgagga gatcgctgcc acgctcaact ggccgctcca
     2341 ggcggtgcgg tggacctgta cgcacccgct catcacatgg gtgctggacg aactcgacgc
     2401 aagtaccacc ccggccgact ccccgccacg aaaacggacg ggcacgaagg atgctgcgtt
     2461 cgcagtgggc tacgcgatcg ggctggtctc aggccccatc cgtttcctgg tcatggtcgt
     2521 ggtgggcgga gccattgtgt tgggcttcgc ccacgcgctt ggattcttct ggcggattct
     2581 gcccgacggc taatcccctg gcgagatgtg aaccgctctc ccgtgctctc cgctgctaga
     2641 gagcgaagtc aaggtcaggg gcttccctcc cgcagcatcc cgaccagcac cgcccgcagc
     2701 gtccggccat cctcgcagtc gatcaggagt tcggtgaaaa gtccgtgcgt tacggcggtg
     2761 cgaccgccgc tccgggtaac ttccttaccc ccgcggcttc tcgtccctcg ccacgtctgc
     2821 cggaacgccc tcacgcttgg ctacgacggt gaaacggtcg ttctcgaaga cgatccactc
     2881 gatg
//
LOCUS       DASTUV010000474         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1645949, whole genome shotgun sequence.
ACCESSION   DASTUV010000474 DASTUV010000000
VERSION     DASTUV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645949"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFT03_09615"
     CDS             complement(<1..400)
                     /locus_tag="VFT03_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEU4848446.1"
                     /translation="MSLNLRELLEDRILIGDGAMGTLLGERGVGFGHPYARANLSHPE
                     MVAGIHEEYLRAGASVVETNTFSANRFKLEIHDLEERVREVNVAGARLARGAVREAGG
                     EGALVLGAIGPLGRPLVPVGPVSQEEAWEVF"
     regulatory      complement(470..579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(618..1577)
                     /locus_tag="VFT03_09620"
     CDS             complement(618..1577)
                     /locus_tag="VFT03_09620"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEU4848447.1"
                     /translation="MRLDPAGGNRFDAGLSLAESYELCRRAQRAHSRTYYFSTRLFPP
                     EVRPHVHALYAFMRYADEIVDNPGETSLEEQLAALEAFEQETLAAVAGEAVPNPVLRA
                     YANTVRVRGIAPEDIATFMTSMKMDTRIFRYRTYEDLAVYTYGSAAVVGLMMCRVVGV
                     VDRRADLHAETLGVAMQLTNFLRDIAEDWRRGRVYVPLEDLERFGYDEQELGDGVVDE
                     RFVELMRFEIGRTRRLYALADDGLDYIPRGRRFPVAVARELYAGILDRIEAQGYDVFA
                     RRAETSRAGKLWVAARCAARDPGDILRGGRLGRRASIALAPRS"
     gene            complement(1582..3039)
                     /gene="crtI"
                     /locus_tag="VFT03_09625"
     CDS             complement(1582..3039)
                     /gene="crtI"
                     /locus_tag="VFT03_09625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEU4848448.1"
                     /translation="MGAGMGSLAAAIRLAAKGFEVEVFEKNDQLGGRMGRLEGSGFTF
                     DTGPSLLLMTDTYRELFASAGRDLDDYVRLIPLDGQYRVTFGDGDVLTIRRALPELIR
                     ELERIEPGVTPRFYRFLEDACNKYRTGRSEFVERDFERARDFFGPRNLKLLLRTRALN
                     NYYRSVSRFFRTDKLRQAFSLQTMYLGLSPFEAPAVYSLLPYTELAEDGLWFPEGGMY
                     SLIEAMERLATELGVTFHLNSPVEEVVVSKGRASGVRVGGEEVWADAVLVGADLPYAY
                     RELLGGSADGDFRLRRREKLEYTASAFMLYLGIDRRLGHMLHHNFYLAGRYRENFEAI
                     FRDHVLPEDPSFYAVVPSKTEPRLAPQGKESLFVLVPVPHLGGRVDWERDGTAFKEKV
                     YQLLEKRCGVKRDWVVFEKVRTPIDWRSEYNLEEGAAFGIGHGILQVGYLRPPMVSKG
                     VEGLYFVGASTRPGTGVPLVTIGARLVAERIGREV"
     gene            3178..4344
                     /locus_tag="VFT03_09630"
     CDS             3178..4344
                     /locus_tag="VFT03_09630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU4848449.1"
                     /translation="MEPGAVVRDRGAARARSGHPWIYRSDVVEAAGEPGDVIPVFDRA
                     GSFLGRAFYNPESEISLRIAEHRDEPVDESWFRTRIDRALAYRDSLAIDGDAYRLLHA
                     EADGIPGLVVDRYGDYLVLQVGSAAVERRLDWVLDALEELRSPAGVLLRGDSAARKRE
                     GLDTAVRVLRGEVPETVVVREGPVRYEADLWRGQKSGGFLDQRENHFAAGRYARGAGS
                     VLDIFCYAGGFALHAARQAGEVEAVDSSGPALGAARRNAELNDLDNITFTRARAFDLL
                     RERSDAGKGYDMVILDPPAFAKTRRDLPQARRAYKEINLRAMKLLNPSGILITCSCSY
                     HLTRELFEDTLRSAAGDAARTLRVREWRGQSDDHPEILTIPETRYLKCAVLEYS"
BASE COUNT          715 a         1509 c         1433 g          752 t
ORIGIN      
        1 gaaatacctc ccacgcctcc tcttggctca ctggaccaac ggggacgagc gggcgtccga
       61 gcggcccgat cgcgccgagc accagcgccc cctcgccgcc cgcctcgcga acggccccgc
      121 gggcgagccg ggcgccggcg acgttcacct cgcgcaccct ctcttcgagg tcgtggatct
      181 cgagcttgaa tcggttggcc gagaaggtgt tcgtctcgac gacgctcgcc ccggcgcgca
      241 ggtactcctc gtgtatgcct gcgaccatct cggggtgtga gaggttcgcc ctggcgtagg
      301 gatgtccgaa accaaccccg cgctcgccga gtagcgtgcc catagcgccg tccccgataa
      361 ggatacggtc ctcgagcagt tctctgaggt taaggctcaa taaatggccc ctctccgtgt
      421 ctttgtttcg cgctcacgcg gtggagagag gcttcgtcga tgttgcttcg actcatctct
      481 cggagtctcc tccgcgggag ttggcacctg acacgaggct tggcctcgcc ggttgccgcg
      541 gcttctcggg gcccgtccct ccaccgctct cgatgagcgc cgccggtcgt ccggcgtctg
      601 tttgagcagc aattacatca cgaacggggc gccaatgcaa tgcttgcccg gcggccgaga
      661 cggccgccgc gcaggatgtc ccccgggtcc cgggcagcgc acctggcggc gacccagagc
      721 ttgccggccc tggaggtctc ggcccgccgc gcgaagacgt cgtagccctg cgcctcgatg
      781 cggtccagga tacccgcgta gagctcgcgc gccactgcca caggaaatcg gcgcccgcgc
      841 gggatgtagt ccaacccgtc gtcggcgagc gcgtacagcc tccgcgtgcg cccgatctcg
      901 aatcgcatca gctcgacgaa ccgctcgtcc acgacgccgt ctcccagctc ctgctcgtcg
      961 tagccgaagc gctccaggtc ctcgagcggc acgtagacgc gccccctgcg ccagtcctcc
     1021 gctatgtccc gcaggaagtt ggtgagctgc atcgccacgc ccaacgtctc ggcgtgcagg
     1081 tcggccctcc tgtctacgac ccccacgacc cggcacatca tcaggcccac cacggcggcg
     1141 ctgccgtagg tgtagaccgc gaggtcctcg taggtccggt agcggaagat gcgggtgtcc
     1201 atcttcatgc tcgtcatgaa ggtggctata tcctccggcg cgatgccgcg cacgcgtacg
     1261 gtgttggcgt aggcgcgcag cacggggttt ggtaccgcct cgccggccac cgccgccaaa
     1321 gtctcctgct cgaaggcttc gagcgccgcg agttgctcct cgagggaggt ctctccgggg
     1381 ttgtccacga tctcgtccgc gtagcgcatg aacgcgtaga gcgcgtggac gtgcgggcgc
     1441 acctcgggtg gaaagagccg ggtcgagaag tagtaggtcc tgctgtgggc ccgctgcgcg
     1501 cggcggcaga gctcgtagct ctcggcgagc gagagccctg cgtcgaacct gttcccgcca
     1561 gcggggtcca ggcgcaaagc cctatacctc gcgcccgatg cgctcggcga ccagccgtgc
     1621 cccgatggtc acgagcggca cccccgttcc cggacgggtg ctcgctccga cgaagtacaa
     1681 accctccacg cccttcgata ccatcggagg ccttaagtac ccgacctgca ggatgccgtg
     1741 cccgatgccg aaggccgccc cctcttccag gttgtactcg cttctccagt cgatcggagt
     1801 cctgaccttc tcgaagacga cccagtccct cttcaccccg caccgcttct cgaggagctg
     1861 gtagaccttc tccttgaagg ctgtcccatc tctctcccag tccacccgac ctccgaggtg
     1921 cggcacgggg acgaggacga agaggctctc tttaccctga ggtgccaggc gcggctcggt
     1981 cttcgacggg acgacggcgt agaaggaagg gtcctcaggg aggacgtggt cgcggaagat
     2041 ggcctcgaag ttctccctgt agcgccccgc gaggtagaag ttgtggtgga gcatgtgccc
     2101 tagcctgcgg tctatgccca ggtagagcat gaacgccgag gccgtgtact cgagcttctc
     2161 gcggcggcgg agcctgaagt ccccgtctgc cgagccacct agcagctcgc ggtaggcgta
     2221 gggaaggtcc gctcccacga gcacggcgtc ggcccagacc tcctccccac ctacccgcac
     2281 gccgcttgcc ctgcccttcg agacgaccac ttcctctaca gggctgttca ggtggaacgt
     2341 gacgccgagc tcggtcgcga gccgctccat cgcctctatg agggagtaca tcccgccttc
     2401 ggggaaccag aggccgtcct cggcgagctc ggtgtagggg aggagggagt agaccgcggg
     2461 ggcctcgaag ggggagaggc cgaggtacat cgtctgcagg ctgaacgcct gtctcagttt
     2521 atccgtgcgg aagaaacgag agacggagcg gtagtaattg ttcagcgccc tcgtcctgag
     2581 caggagcttg aggtttctcg ggccgaagaa gtcgcgcgcg cgctcgaagt cccgctcgac
     2641 gaactcgctt cggccggtac ggtacttgtt gcaggcgtcc tcgagaaagc ggtagaagcg
     2701 cggcgtgacg cccggctcga tgcgctcgag ttccctgata agctcgggca gggcgcgtcg
     2761 gatcgtgagg acatcgccgt ccccgaaggt tacgcggtac tgaccgtcga gtggaatgag
     2821 ccttacgtag tcgtcgaggt cgcgtccggc ggaggcgaag agctcgcggt aggtatccgt
     2881 catgaggagg agtgaggggc ccgtgtcgaa cgtgaagccg ctaccctcga gcctgcccat
     2941 ccggccgccg agctggtcgt tcttttcgaa gacctccacc tcgaagccct tcgcggcgag
     3001 ccggatcgcc gccgcaaggg agcccatccc cgctcccacg accgctatac gcttcgcccg
     3061 cgacgcttcc agaacgcccg cctttcttac gaggatatat taagtatacg gtgtcgatac
     3121 cagtgcggat gctcacagag gttcgccgga gaacttggta aaggagctta cagcagcgtg
     3181 gaaccagggg ccgtggtaag ggacaggggc gccgcgcgcg cccgttcggg gcacccctgg
     3241 atctaccgct ccgacgtcgt cgaggccgca ggtgaacctg gcgacgtaat ccccgtcttc
     3301 gaccgggcag ggagcttcct cggccgcgcc ttctacaacc cggagagcga gatctccctt
     3361 cgcatagccg aacaccgcga cgagcccgtg gacgagtcct ggttccggac ccggatcgac
     3421 cgcgccctag cctaccgcga ctcgctggcc attgacggcg acgcctaccg cctcctgcac
     3481 gccgaggccg acggcatccc cggcctcgtg gttgaccggt acggtgatta cctggttttg
     3541 caagtcggct ccgcggccgt cgagaggcgg ctcgactggg tcctggacgc cctcgaagag
     3601 ctgcgctcgc ctgcgggggt gctcctgcgg ggcgactccg ccgccaggaa gagggagggc
     3661 ctcgacaccg cagtacgtgt gctgcgcgga gaggttcccg agaccgtcgt cgtccgggaa
     3721 ggccccgtgc gctacgaggc ggacctgtgg cgggggcaga agagcggcgg tttcctcgac
     3781 cagcgcgaga accacttcgc cgccgggcgc tacgccagag gtgcaggcag cgtgcttgac
     3841 atcttctgct acgcgggcgg attcgccctg cacgccgcgc gccaggccgg ggaggtcgag
     3901 gccgtggact cgagcggtcc cgcgctcggg gcggcgcgcc gaaacgcgga gctcaacgat
     3961 ctcgacaaca tcaccttcac ccgtgccagg gcctttgatc tgttgcgcga gcgctccgac
     4021 gccgggaagg gctacgatat ggtcatcctc gatcctcccg ctttcgccaa gacgaggcga
     4081 gatctcccgc aagcgcggcg ggcctacaag gagatcaacc tccgggcgat gaagctgctt
     4141 aacccgagcg gcatcctcat aacctgctcc tgctcatacc acctcacgcg ggagcttttc
     4201 gaagacactc ttcgctctgc agccggggac gccgcgagga ctctgcgggt gcgggagtgg
     4261 agggggcagt ccgacgatca cccggagatc ctgacgatcc ccgagacccg ctacctcaaa
     4321 tgcgccgtgc tcgagtactc ataacgccac atcgccacgt gcaccttccg ggtagagcta
     4381 ccggcgatcg gtgtcctgca ggcgttctg
//
LOCUS       DASTUV010000475         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1072688, whole genome shotgun sequence.
ACCESSION   DASTUV010000475 DASTUV010000000
VERSION     DASTUV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072688"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFT03_09635"
     CDS             <1..295
                     /locus_tag="VFT03_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4848450.1"
                     /translation="YDAAAFITLMRPNVWIEISGLPPKRLPDYYRNFDFERLAQKMIF
                     GTDWPGVPGPRSNAQAIAGLDLGHETLQKIFYKNALHVYERMGAGKLTTIDID"
     gene            343..1290
                     /locus_tag="VFT03_09640"
     CDS             343..1290
                     /locus_tag="VFT03_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEU4848451.1"
                     /translation="MTAVTEEILLDRIKSGRLIEDSSQATERYIEGLKRTLIVSADTE
                     LISAPAYMNATKDFSTLPSINNYITVMGIVQDELGHAHIAYRMLRDLGVDTEDLVYER
                     KPRRFKYPYAFDVPLESFTELIVANGFYDRAGFVLLSDIHHNCSYGPWKRALVKVDRE
                     ENFHLRHGEKWMKAYSRDPEKKEELQRAVDWMFPLTLEWFGLPDGQKRHNEQIEYGYK
                     GSTNDQLRQTWMSHTVPLCESLGLDVPAHYDEEADHYVIDFPFPARFDAGEKRWKFEE
                     GEISWDDVLVRWRERGPKNEEYVEHLQRGYKELYATEAV"
     gene            1293..1622
                     /locus_tag="VFT03_09645"
     CDS             1293..1622
                     /locus_tag="VFT03_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU4848452.1"
                     /translation="MQEKIIAAGVTSEEARDALREVFDPEYPISLVDLGLIRGVEVEG
                     SRAEIKLTYTCMGCPAMDMIQDDVRERLLEMDGIDEVDIEVVWETWSRKDITPLGKKQ
                     LKSVGVI"
     gene            1622..1816
                     /locus_tag="VFT03_09650"
     CDS             1622..1816
                     /locus_tag="VFT03_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848453.1"
                     /translation="MRPVENYEVFTARVGEAPLRHNGNVRAAEPRDAAVYAHLMYDEW
                     RWREMFVVPSEAIVRVIRPD"
     gene            1826..2035
                     /locus_tag="VFT03_09655"
     CDS             1826..2035
                     /locus_tag="VFT03_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848454.1"
                     /translation="MERYLVFARTEYAEPLEHRGDVEAASDGEAARLAQERYGRDWLE
                     MSLVPVSKAYWAEKETEEGETEVEV"
BASE COUNT          469 a          608 c          756 g          352 t
ORIGIN      
        1 gtacgacgca gcagcattta tcaccctcat gcgccccaac gtctggatcg agatctccgg
       61 ccttcccccc aagaggctcc cggactacta caggaacttc gatttcgagc ggctggccca
      121 gaagatgatc ttcggtaccg actggcccgg cgtgccaggc ccgagatcca acgcccaggc
      181 catcgctggc ctcgacctcg gtcacgagac actacagaag atcttctaca agaacgccct
      241 ccacgtttac gaaagaatgg gggcgggtaa actgacaacg atcgatattg actgaccgga
      301 cagttattat gttgcagtcg ggtcagacag gaggagaaag gcatgacggc ggtaactgag
      361 gagatactcc tggatcgcat caagagtggg aggctcatcg aggactcttc ccaggcgacg
      421 gagaggtaca tcgagggttt gaagcggacc ctgatcgtct cggctgacac cgagctgata
      481 agcgctccgg cgtacatgaa cgcgacgaag gacttctcca cgctccccag catcaacaac
      541 tacatcaccg tcatggggat agtgcaagac gagctcgggc acgcgcacat tgcctaccgc
      601 atgctgaggg atctcggggt cgacacggag gacctagtct acgagcgcaa gccccggcgg
      661 ttcaagtacc cctacgcctt cgacgtgccg ctggagagct tcacggagct catcgtcgcc
      721 aacggcttct acgacagggc cggcttcgtt ctgctcagcg acatccacca caactgttca
      781 tatgggccgt ggaagcgggc gctcgtcaag gtggaccgcg aggagaactt tcacctcagg
      841 cacggcgaga agtggatgaa ggcgtactct agggacccgg agaagaagga ggagctccag
      901 cgggcggtcg actggatgtt cccgctgacg ctcgagtggt ttgggctgcc ggatggacag
      961 aagcggcaca acgagcagat cgagtacggc tacaagggct cgaccaacga ccagctcagg
     1021 cagacctgga tgagccatac ggtgcccctg tgcgagtccc tcgggctcga cgtgccggct
     1081 cactacgacg aggaagcgga ccattacgtc atagacttcc cgttcccggc gcggttcgat
     1141 gccggggaga agcggtggaa gttcgaggag ggtgagatct cctgggacga cgtgctggtc
     1201 cgctggaggg aacgcggtcc caagaacgag gagtacgtcg agcaccttca gcgcggctac
     1261 aaggagctct acgccacgga ggccgtctag cgatgcagga gaagatcatc gctgcgggcg
     1321 tcacctcgga ggaagcgcgg gatgcgctcc gggaggtatt tgacccggag tacccgatca
     1381 gcctggtcga cctcggcctc atccggggcg tcgaggtgga ggggtccagg gcggagataa
     1441 agctgacgta cacctgcatg ggttgtccgg cgatggacat gatccaggac gacgtccgcg
     1501 agaggctcct ggagatggac ggcatagacg aggtggacat agaggtcgtg tgggagacgt
     1561 ggtcgaggaa agatattacg cccctgggca agaagcagct caagagtgtg ggggttatct
     1621 gatgcggccg gtcgagaact acgaggtatt tacggcgcgc gtgggcgaag ctccgcttag
     1681 gcacaacggc aacgtcaggg ccgcggagcc gagagatgcg gccgtctacg cgcacctgat
     1741 gtacgacgag tggcgctggc gcgagatgtt cgtcgtaccg agcgaggcca tcgtccgggt
     1801 catcaggccg gattagggag gagtggtgga gagatacctg gtcttcgcgc gcaccgagta
     1861 cgccgagccg ctggagcaca ggggcgacgt cgaagccgcc agcgacgggg aggcggcgag
     1921 gctcgcccag gagcgctacg gaagagactg gctcgagatg tcgctagtgc cggtgagcaa
     1981 ggcttactgg gcagagaagg agaccgagga gggcgagacg gaggtggagg tgtgagcgag
     2041 cggccggaac gcacggagga ggtggcgcct gggcgcggcg ccgaaaatgc gttcctcgtc
     2101 gagaacgacg aggccctggc cgcgctggtc aacctgatcg cggtcctggg agacaacgtg
     2161 tacttcctgg gtcgccgcgt ctccg
//
LOCUS       DASTUV010000476         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_419475, whole genome shotgun sequence.
ACCESSION   DASTUV010000476 DASTUV010000000
VERSION     DASTUV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419475"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VFT03_09660"
     CDS             complement(<1..1250)
                     /locus_tag="VFT03_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU4848455.1"
                     /translation="MLLLLLCKEWTPRQHKGGEIKVYEAVAETLSRLGVENVFGLVGS
                     GNFGLVDYITRNCGVAFHASRHEEAGVAMADGYARVSGKLGVCTVHQGPGVTNTLTAL
                     TEAVKNRTPLLLLAGDTATTAIYQNLDVDQAAVVRSVGAGVEHIRAAHTAVEDAARAA
                     RRAEIERLPIAVSLPIDLQEQTCEVIEPPAFAEEPIPAPRPSGEAVARAADLLEASSR
                     PAIIAGRGAVLAGARPQLEALGGRLGALFATSMMARGFFSGSPFDLGVSGGFASPLAA
                     RLLGEADVVLSFGASLNTWTTKHGSLFAPSAGIVQCDLEASAIGRIQPVTLGVIGDAA
                     ETAGALLDELDRRGVSLEGFRTEAVVRDIESFRWEDEFEEDRAEGTVDPRSVLMALDG
                     ILPEERNVAVDCGHFAGFPARHLRV"
     gene            1407..1661
                     /locus_tag="VFT03_09665"
     CDS             1407..1661
                     /locus_tag="VFT03_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848456.1"
                     /translation="MLVSGITAVIHGREEPFGVLCAPSNFNRAFTEDDISFTQAVANV
                     LGGARGGKGIGVSTAFEAEGTKSPLYRIDCGFVGVRERTR"
     gene            1668..2501
                     /locus_tag="VFT03_09670"
     CDS             1668..2501
                     /locus_tag="VFT03_09670"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU4848457.1"
                     /translation="MISGKTRLLALIGHPVEHSLSPAMHNASFEADGLDYVYVALDVK
                     PDDLSLAVRGAPALGFRGFNVTMPHKRAILPFLDGVDEGARLSGAVNTVVVEDATLRG
                     YNTDGGGLVEACREAGIDLVGRRVLLLGAGGVAAAIAFAFGGEEIGELRIANRTLEHA
                     IELRDKLRGAGLKQPEVYPPYALDEAAREVEVIVNATPLGMKDGDPLPVPIEHLDEGR
                     VVCDAVYRPGRDTGLIREARERGARVVTGERMLLYQGVLAQRLWTGREPNVKAMDGAL
                     A"
     gene            complement(2509..>2791)
                     /locus_tag="VFT03_09675"
     CDS             complement(2509..>2791)
                     /locus_tag="VFT03_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848458.1"
                     /translation="FAEYLTFDRVVPFEWPSLAADSLGNILTAQGQGEAGYMYHEETA
                     TPVLGIAIPAGMVICGSTSGMLRSLESGLRQRLIQLVKSRSGEDRDVPT"
BASE COUNT          482 a          907 c          924 g          478 t
ORIGIN      
        1 acccggagat gcctggcggg gaagcccgcg aagtgaccac agtccactgc cacgtttcgc
       61 tcttccggga gtatcccgtc gagggccatg agaacggagc gcggatctac cgtcccctcg
      121 gcgcgatctt cctcgaactc gtcctcccag cggaagctct cgatgtcccg cacgaccgct
      181 tccgtcctga acccttccag gcttacgcct ctgcggtcga gttcatcgag cagcgcaccg
      241 gctgtctcgg cggcgtcgcc gatcacgccg agcgtcaccg gctgtatgcg gcctatcgcc
      301 gacgcttcca ggtcgcattg gacgatcccg gccgacggag cgaagaggct cccgtgtttg
      361 gtggtccacg tgttgaggct ggcaccgaac gagagcacga cgtccgcctc gccgagcagc
      421 cgcgccgcga gtggggaggc gaaaccgccg gagacgccga ggtcgaatgg gctgccggag
      481 aagaagcccc tggccatcat cgaggtggcg aagagagccc cgagcctgcc cccgagcgcc
      541 tccagttgcg ggcgcgcccc cgccagcacc gcgcctcgcc cggcgatgat cgccggcctg
      601 ctcgacgcct ccagcaggtc ggcggcccgc gcgaccgcct cacctgaagg tcgcggagcc
      661 gggatgggct cttcggcgaa agcaggtggc tctataacct cgcaggtctg ttcctgcaga
      721 tcgatgggga gggagactgc tatgggaagc cgctctatct cggcccgccg cgccgcccgt
      781 gccgcatcct cgacggctgt gtgcgcggct cgtatgtgct cgacacccgc tccgaccgag
      841 cgtacgacgg cggcctgatc tacgtccagg ttctggtaga tcgccgtggt cgccgtatcc
      901 ccggccagga ggagcagagg ggtccggttc ttcacggcct ccgtaagcgc ggttagcgtg
      961 ttcgtgaccc ctggcccctg gtgtaccgtg cagactccca gcttgccact cacgcgggcg
     1021 tatccgtcag ccatggcgac gccggcctcc tcgtggcggg aggcgtggaa ggctactccg
     1081 cagtttctcg ttatgtagtc cacgagaccg aagttcccgc tgcctaccag cccgaagacg
     1141 ttctcgaccc cgagccggct cagggtctcg gctaccgctt cgtatacttt gatctctccc
     1201 cccttatgct gcctgggcgt ccattcctta cagagaagca ggagaagcag gcgcgcagaa
     1261 caagactggc ccgcactgcc ctatccacct ataacgttat atccacagca gtggaccggg
     1321 atctacgcga ccttcgatgc ggcgcccctc tccatcttgt cctttcctcc gcttgctatt
     1381 ctggctgcta tggagccacc gcgcaaatgc tggtcagcgg cataaccgca gtcatccacg
     1441 gccgtgagga gcccttcggg gtgctctgcg ccccctccaa cttcaaccgt gccttcaccg
     1501 aggacgatat aagcttcacg caggcggtgg ctaacgtgct gggtggggcc agaggaggta
     1561 agggaattgg cgtaagcacc gccttcgagg ccgaaggcac gaaaagcccc ctgtatcgga
     1621 tagactgcgg ttttgtaggg gttcgcgaaa ggacgaggtg agagtggatg atcagcggta
     1681 agacgcggtt gctcgccctg atcgggcacc ccgtcgagca tagcctgagc ccggctatgc
     1741 acaacgcctc cttcgaagcc gacggtctgg actacgtcta cgtcgctctc gacgtgaagc
     1801 cggatgacct gtccctggcg gtgaggggag cgccggccct cgggttccgg ggcttcaacg
     1861 tcaccatgcc ccacaagcgg gcgatactcc cgttcctgga cggcgtggac gaaggagccc
     1921 ggctctccgg agccgtgaac accgtggtgg tcgaggacgc gacgctccgc ggctacaaca
     1981 ccgacggcgg cggcctcgtg gaggcttgca gggaagcggg catcgacctg gtgggcaggc
     2041 gggttctgct cctgggcgcc ggaggagtgg cggccgccat cgccttcgct ttcggcggag
     2101 aggaaatagg agagctgcgc atcgccaacc ggaccctaga acacgctata gaactgcggg
     2161 acaagctacg cggggcgggc ttgaagcagc ccgaggtata ccctccctat gcactggacg
     2221 aggcggcccg agaggtagag gttatcgtga atgcgacgcc gctagggatg aaagatggcg
     2281 accctcttcc ggtgccaata gaacatctgg acgagggcag agtggtctgc gacgcggtgt
     2341 accgtcccgg cagggatacc ggcctgatcc gggaagcccg cgagcgaggc gccagggtcg
     2401 tcacaggcga gcgtatgctg ctttaccagg gcgtgctagc gcagcggctc tggacgggcc
     2461 gggaaccgaa cgtgaaggcg atggacggtg cgctcgcctg agcgcaggct aggtcggtac
     2521 gtcccggtct tcgccggagc ggcttttcac gagctgtatt agccgctgcc tgaggcccga
     2581 ttcgagggac cgcaacatcc cggaggtcga gccgcagatg accataccgg cagggatcgc
     2641 gatgcccagc acgggggttg ccgtctcttc gtggtacatg tagcccgctt cgccctgacc
     2701 ctgggccgtc aggatgttcc cgagagagtc ggcggcgagc gagggccact cgaaaggaac
     2761 gactctgtcg aacgtgaggt actcggcgaa c
//
LOCUS       DASTUV010000477         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1300872, whole genome shotgun sequence.
ACCESSION   DASTUV010000477 DASTUV010000000
VERSION     DASTUV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300872"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFT03_09680"
     CDS             complement(<1..267)
                     /locus_tag="VFT03_09680"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEU4848459.1"
                     /translation="MAIVILFARLLLAAVFVVAGVAKLADREGSRRAVADFGVPGALA
                     APLSVLLPLAELAVAVALIPTSTAIWGAVGALALLLLFVAGIGAN"
     gene            complement(601..1701)
                     /locus_tag="VFT03_09685"
     CDS             complement(601..1701)
                     /locus_tag="VFT03_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU4848460.1"
                     /translation="MRVLRQRGLPGGDGPHARRPGPGGPPDRGPRRRVELERENLSAG
                     IPEDAPLVALRRGAIVESVHRGRFAVCDPEGNVVEAVGDPEGYVYARSSAKPFQALPL
                     LLSGAADALGLTDEELAVACASHNAEEPHLRAVRSILEKAGLSEDDLQNGAHPPLYAP
                     AAMELACRGKEPAAIHGNCSGKHAGMLAVCVHEGWATSGYRNPDHPLQVWILEIVARV
                     CGLEPDEILLGGDGCGVPSFGMPLANLATGFARLATCALPGDLAEAANKVSGAMREHP
                     YMVGGTERFDTAVMRGTDLVCKSGAEAVFGSGSPEGWGLALKISDGGTRALSPATLAA
                     LSRRGVEVHGEPESSPVRDLHSQVVGECAPLF"
     gene            complement(1599..1919)
                     /locus_tag="VFT03_09690"
     CDS             complement(1599..1919)
                     /locus_tag="VFT03_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848461.1"
                     /translation="MRELGEERATTNLRVGHLLRITGYVEAATIAMWTGSPRALVMMG
                     MAEASVRGEGPGGKDEDLLVKLRPMVGEACEYYASGDFPAAMARMRVAQDLVDLRIVA
                     LAGE"
     gene            complement(1916..2746)
                     /locus_tag="VFT03_09695"
     CDS             complement(1916..2746)
                     /locus_tag="VFT03_09695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4848462.1"
                     /translation="MSETERKHGIETLTSGAMAGVWAWVRDRLPQGGGVFDAHAHIGV
                     DLDGRTMTAEVMRERMLAAGVARSIVFPLNDPNARDDYSGPNDVIWKAHEDHPGIFVP
                     FFRLNPHLDYEKEFERCAERGFMGLKLHPVSQRFELDDERVARLLGKAAEADLPVLIH
                     TGFAMRRVVKPLLPTLEAHPELRLILGHASMVEVLEAARAFEDNTNVLFETSVVSAKD
                     LYVLFSSVEAGRVCYGSDIPYGDIPSTLHAILRAAEAAGVPEKQFPAVLSGNIRRWFS
                     "
BASE COUNT          476 a          980 c          927 g          467 t
ORIGIN      
        1 gttggcgccg ataccggcaa cgaagaggag caggagtgcc agagcaccta cggctcccca
       61 gattgccgtg gaggtgggga tcaaggcgac ggccacggca agctcggcca gaggcagcag
      121 gacgctcagc ggagcggcga gggcgcccgg taccccgaag tcggctaccg cccggcgaga
      181 gccttcgcgg tcagcgagct tggcgacccc ggccacgacg aagactgcgg cgagcaacaa
      241 cctcgcgaag agaatgacta tggccatagt ggctctcctt acgtatttgt gagttctacc
      301 aagcggatat cgcgcctctt tcaactatag catctgcatg tggcgaccat ccacgccgag
      361 tgctgagtac gccctcagat cgagcctcgt ccctcttatc acgttctcgt gcggggagac
      421 gcactttctg cgccgctagc aacaattaga aacccccaag agcttgcaaa gatcaccctt
      481 ttgggggatg tgcccgctcc tcgggtgagt gtacgctatc agatgctgcg cagcctgggg
      541 cggggcttag gcgcatcttc tccccgcccc cgttctgttc cgggggtcct gctacgccat
      601 tcagaacagc ggcgcgcact ctccgacgac ctggctgtgc aggtcgcgca ccggggatga
      661 ctccggctcg ccatggacct caactccccg gcgggagaga gcggcgaggg tggccgggct
      721 aagggcgcgc gtcccgccgt cggagatctt gagcgccagc ccccagccct ccgggctccc
      781 cgagccgaag accgcctccg ccccgctctt gcagacgaga tccgtgccgc gcatcacggc
      841 cgtgtcgaag cgctccgtcc cgccgaccat gtagggatgc tcgcgcatcg ccccgctgac
      901 cttgttcgcg gcctcggcca ggtcgccggg gagggcgcag gtcgcgagcc tggcaaagcc
      961 ggtagcgagg ttcgctagcg gcatgccgaa agagggaacg ccgcacccat cgccgccgag
     1021 cagtatctcg tccggttcga gaccgcagac tcgggccacg atctcgagta tccacacctg
     1081 caaggggtgg tcggggttgc ggtaaccact cgtcgcccaa ccttcgtgga cgcacacggc
     1141 gagcatcccg gcgtgcttgc cagagcagtt gccgtggatg gccgcgggtt ccttgcccct
     1201 gcatgcgagc tccatcgcag cgggggcgta gagcggcggg tgcgcgccgt tctggaggtc
     1261 atcctcggag aggccagcct tctcgaggat ggagcgtacg gctctcaggt gcggctcctc
     1321 ggcgttgtgc gaggcgcagg cgacggcgag ctcctcgtcg gtcagaccaa gggcatccgc
     1381 ggcgccggag agaaggagag gaagcgcctg gaagggcttc gccgaagagc gtgcgtagac
     1441 atagccctcc gggtcgccca cggcctcgac gacgttacct tccgggtcgc agacggcgaa
     1501 gcgtccccgg tggacgctct cgacgatcgc tccgcgccgg agggcgacga gcggcgcgtc
     1561 ctcggggatc cctgcagaga ggttctcgcg ctccagctct actctccggc gagggccacg
     1621 atccggaggt ccaccaggtc ctgggcgacg cgcatgcggg ccatcgccgc cgggaagtcc
     1681 ccgctggcgt aatactcgca cgcctccccg accatcggcc tgagcttcac cagcaggtcc
     1741 tcgtccttcc cccccggtcc ctcgcccctc acgctcgcct ccgccatgcc catcattacc
     1801 aacgcgcggg ggctccctgt ccacatcgcg atcgtcgcgg cctcgacgta gccggtgatg
     1861 cgcaacaggt gccctaccct gaggttggtc gtcgcccgct cctcccccaa ctctctcaag
     1921 agaaccaccg ccggatgttg ccagagagga ccgcagggaa ctgcttctcc ggaaccccgg
     1981 cagcttcggc agcgcggagg atggcgtgca gagtcgaggg tatgtcgccg taggggatgt
     2041 ccgagccgta gcagacgcgc ccggcttcga cggagctgaa gagcacgtag aggtccttcg
     2101 cgctcaccac cgaggtctcg aaaaggacgt tggtgttgtc ctcgaaggcg cgcgccgctt
     2161 cgaggacctc gaccatcgag gcatggccca ggatcaggcg cagctcgggg tgcgcctcga
     2221 gcgtcgggag caggggtttc accaccctcc gcatcgcgaa accggtgtgg atcaggaccg
     2281 gcaagtccgc ctctgcggcc ttgccgagca agcgcgccac ccgctcgtcg tcgagctcga
     2341 acctctggga gacagggtgc agcttcagac ccatgaagcc gcgctctgca caccgctcga
     2401 actccttctc gtagtcgagg tgcgggttga ggcggaagaa cggtacgaag atgcccggat
     2461 ggtcctcgtg ggccttccag ataacgtcgt tcggcccaga gtagtcgtcg cgcgcgttcg
     2521 ggtcgttcag gggaaagact atgctcctcg caacccctgc ggccagcatc ctctcgcgca
     2581 tcacttccgc agtcatcgtc cggccgtcca aatccacccc gatgtgggcg tgggcgtcga
     2641 agacgccacc cccctgtggc agcctgtcgc ggacccacgc ccagaccccg gccatcgccc
     2701 cgctagtcaa ggtctcgatg ccgtgttttc tctcggtctc gctcaaggac gcgtccgccg
     2761 atctggtgcg cgatcacgac cgtgaaatgt tagcaggagg tagcggaggt tgccgcgccg
     2821 gaccaggtca ggcgcagccg ttcaacctcc
//
LOCUS       DASTUV010000478         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_762461, whole genome shotgun sequence.
ACCESSION   DASTUV010000478 DASTUV010000000
VERSION     DASTUV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762461"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VFT03_09700"
     CDS             <1..1188
                     /locus_tag="VFT03_09700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4848463.1"
                     /translation="ELAKRMERLGTETAFSVLAKAKALEAQGREIIHLEIGEPDFDTP
                     SHIIEAACRALRGGHTHYTPTAGIPELREAIAADVARSRGIEVEPAQVVVTPGGKPIM
                     FFAILALVEKGDEVLVPNPSFPIYESMVNFAGARPVFVPLRQENEFRFDLDELRAGLS
                     EHTKLVILNSPANPTGGVLTPEDIAGLAAILRERPDVYVLSDEIYSRMLYDSPFASIA
                     SEPGLGPDDRTIILDGFSKTYAMTGWRLGYGVMPIPLAEHVEKLQVNSNSCTNAATQH
                     AGLEALAGPQDSVDAMLAEFRARRDLIVAGLNDLPGVDCITPRGAFYAFPRVTDTGYA
                     ANSLADLLLEEAGVACLAGTAFGRYGEGHLRLSYANSRENIALALEHMHRILSRTRPF
                     LSS"
     gene            1315..1926
                     /locus_tag="VFT03_09705"
     CDS             1315..1926
                     /locus_tag="VFT03_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848464.1"
                     /translation="MATRTHKNVEKMGDTAHEMTRSAQRTAQIFSGYLTEAQEINTDY
                     ARRATETWIEAFRKQTELNQRMVQRLYGEAEGQSGAFQGLMQDWVRMYSVPFFNPFGA
                     ENPFVKGNPFADYFRQGVETATRNAERVTNMTQRATAAGIVSTNGGFPIAGYDDKNVA
                     EISSMLDGLSEEQLRTVREYERRHKNRDSLIEQIDRKIKATNA"
     gene            2103..2303
                     /locus_tag="VFT03_09710"
     CDS             2103..2303
                     /locus_tag="VFT03_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848465.1"
                     /translation="MEVISGAGVGGEMESHFIGVHGPYTGVPDSVPLGDSGIPEACGD
                     DAPLEGACQTCQTQANLRRWTG"
BASE COUNT          480 a          762 c          699 g          387 t
ORIGIN      
        1 gagcttgcga aacggatgga gcgcctgggg accgagacgg ccttctccgt gctggcgaag
       61 gccaaggcgc tcgaggctca gggacgggag atcatccacc tggagatcgg cgagcccgac
      121 ttcgacacgc catcccacat catcgaggcc gcctgccggg cgctgcgcgg cggacacacc
      181 cactacaccc cgacagccgg catcccggag ctgcgcgagg cgatagccgc cgacgtggca
      241 cgtagccggg gcatcgaggt ggagccagcg caggtcgtag tcacgccagg ggggaagccg
      301 atcatgttct ttgccatcct cgcgctcgta gagaagggcg acgaggtcct ggtgccaaac
      361 ccctcgttcc cgatctacga gtcgatggtg aacttcgccg gcgcacgccc ggtcttcgtg
      421 ccgctgcgcc aggagaacga gtttcgcttc gacctcgacg agctgcgcgc tggcctgagc
      481 gagcacacca aactggtgat cctcaactcg ccagccaacc ccacaggcgg cgtgctcaca
      541 cccgaagaca tagcaggact agccgctata ctgcgcgagc gccccgacgt ctacgttctg
      601 agtgatgaga tctactcccg catgctctac gatagcccct tcgccagcat cgcctccgaa
      661 cccggcctcg gccccgacga ccgcaccatc atcctcgacg gcttctccaa gacctacgcg
      721 atgactggct ggcgcctggg ctacggcgtg atgccaatac ccctcgcaga acatgtagaa
      781 aaactccagg tcaactcgaa ctcctgcacc aacgccgcca cccagcacgc cggcctggaa
      841 gccctcgccg gcccgcaaga cagcgtagac gccatgctcg ccgagttccg cgcccgcagg
      901 gacctgatcg tcgccggcct caacgatctc cctggcgtcg attgcatcac cccaagaggc
      961 gccttctacg ccttcccacg tgtcacggac accggctatg ccgcaaattc cctcgccgac
     1021 ctgctcctcg aagaggccgg cgtcgcttgc ctcgctggca ccgctttcgg ccggtacggc
     1081 gaaggccacc tccgcctctc ctacgccaac tcccgcgaaa atatcgccct cgccctcgag
     1141 cacatgcaca ggatcctctc ccgaactcgt cccttcttat cctcataata cccttgatat
     1201 ctggagttcc tgtaataaat tccgggatcg aaggctagga agatgtttag gctctctatg
     1261 tgctgggtac acatacatac agtaggcaag agatacgatt ttggaggtat cgagttggct
     1321 actaggacgc acaagaacgt tgaaaagatg ggcgatacgg cccacgagat gacccgcagc
     1381 gcgcagcgta cggcccagat ctttagcggc tacctcactg aggcgcagga gatcaacacc
     1441 gactacgccc ggcgcgcgac cgagacctgg atcgaggctt tccggaagca gacggagctg
     1501 aaccagcgga tggtgcagag gctctacggc gaggccgagg gccagagcgg cgcctttcag
     1561 ggtctcatgc aggactgggt gcgcatgtac tcggtgccgt tcttcaaccc cttcggcgcg
     1621 gagaacccgt ttgtaaaggg taaccccttc gccgactact tccgccaggg cgtagagacg
     1681 gccacccgca acgccgagcg cgtgacgaac atgacgcaga gggctacggc ggctggaatc
     1741 gtcagtacta acggcgggtt cccgatagcg ggctacgacg acaagaacgt cgcggagatc
     1801 tccagcatgc tggatgggct ctccgaggag cagctcagga cggttcgcga gtacgagcgg
     1861 cgccacaaga accgcgactc gctcatcgag cagatagacc gcaagatcaa ggcgaccaac
     1921 gcttaactgg agttacgctc gcaagagttt ccgagggccg ggggtagatc cccggccctt
     1981 ttcgctgcct gattactcgt ataggccggc tcgatacgag acatagagaa ccgttatttc
     2041 tccgcggcgg ctaactgtgc catatttacg gctcgggttg ctggcgagcc aaggagcaga
     2101 agatggaagt gatctctggc gcgggagttg gtggggagat ggagagccac tttatcggag
     2161 tgcacggtcc ctataccggc gtcccggatt cggtgccgct cggcgactcc ggcatacccg
     2221 aggcctgcgg ggacgacgct ccgctcgaag gcgcgtgcca gacgtgccag acccaggcga
     2281 acctcagacg ctggacaggg taagcgtcag caccgagaga cggggaaa
//
LOCUS       DASTUV010000479         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_794519, whole genome shotgun sequence.
ACCESSION   DASTUV010000479 DASTUV010000000
VERSION     DASTUV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794519"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFT03_09715"
     CDS             <1..443
                     /locus_tag="VFT03_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848466.1"
                     /translation="TDGRDLPLWDRVLELVKEDLVERRSKKLFVEIHSEAGSCRKEIG
                     DLSTAERHSLHPALRQRVVEQLLDRYLAEHGYVDLVKKRRVYTKNIQDLTPEDLPALV
                     EADRRRVEESQEERERADFIREVRAGLERLRVTSSPSGVARVRR"
     gene            447..971
                     /locus_tag="VFT03_09720"
     CDS             447..971
                     /locus_tag="VFT03_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU4848467.1"
                     /translation="MQKTRLRSRMDDMGNREPVLDGGRVPRQCPACAGLSELEHLSDG
                     NHEQVESLFEETLHLMLTLKEKLDVAFCLLGAARNEDRILTSELVLAGGERAPVLKEI
                     SSRDPATQPAGLTPREVEVLRLVATGLTDAQVAEKLFISLRTVNAHLRAIRAKLGVSS
                     RSAVTRYAFEHQLI"
     gene            1353..>2557
                     /locus_tag="VFT03_09725"
     CDS             1353..>2557
                     /locus_tag="VFT03_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEU4848468.1"
                     /translation="MIALVVAGGVFGLSRPADASSHREAPFIGGDPEADATDVYAFRS
                     PDKPNTVTLIANYVPLEVAAAGPNFYKFGEDVLYEINVDNDGDAKDDISFQLDFRNRI
                     NNGDTFLHNTGQITSLDDPDLNQEQFYSLSVLKGDDHNDPSREGKVIAQNLQVAPANV
                     GPKSYPQGYSKVANQAIYTLDNGVKVFAGPRDDPFFADLGGIFDLLQGIEGRDYLKDL
                     NVHTIAVQVPISMLKGPDDSIIGVRTTSYRQSTSVLRPVGDPDSQLDDPRATSGPWVQ
                     LSRLDMPLVNELVIPLKDKNRFNGSQPRFDGQFLTYVRNPEPAGLIENILGIDVPPNP
                     RDDIATIFLTGIQGLNKPTGVKPSSQLRLNMDVQPKASPIRLGVLGGDNAGFPNGRRL
                     TDDVVDIEL"
BASE COUNT          536 a          769 c          784 g          468 t
ORIGIN      
        1 gaactgatgg gagagatcta cccctttggg accgagttct cgagctggtc aaggaggatc
       61 tcgtcgaacg ccgcagcaag aagctgtttg tagagataca cagcgaggcc ggttcgtgcc
      121 ggaaggaaat cggggacctc tccacggcgg agcgtcatag ccttcatccg gctctcaggc
      181 agcgtgtcgt agagcaatta ctcgatcggt atctggcgga gcacggctac gtcgatctcg
      241 tcaagaaaag gcgcgtctac accaagaata tccaggatct tactccggaa gacctaccgg
      301 ccctcgtaga agccgatcgg cggagggttg aggaatccca agaggagcgc gagcgagcgg
      361 actttatccg cgaggtgcga gcgggcctgg agcgactccg tgttacgagt tcgccaagtg
      421 gcgtagccag agtaaggagg tgagtgatgc agaagactcg tctccgcagc cggatggacg
      481 atatggggaa ccgggagccg gtcttggacg gcgggcgggt gccccggcag tgccctgcct
      541 gtgccggcct gagcgagctc gagcatctct cggatggtaa tcatgagcag gtggaatcac
      601 tgttcgagga aaccctacat ctgatgctga cactaaagga gaagctggac gttgccttct
      661 gccttctagg tgcagcgcgt aatgaggatc ggatacttac ttccgagttg gttctcgcag
      721 ggggagaacg agcaccggta ctgaaggaga tcagctccag ggacccggcg acccagcccg
      781 ccggactgac accccgcgag gtggaagtgc tgcgcctggt tgcaacgggt ctaaccgacg
      841 cccaggtggc cgaaaaactc ttcatcagcc tgcgtaccgt caatgcgcac ctgcgcgcga
      901 tccgcgccaa gctgggggtc agctcgcgca gcgcggtcac ccgctacgcc ttcgaacacc
      961 agctcatctg agccttcggc gcccggctat taccggtctt ctcggttctg gtggtcctct
     1021 tgccttatga gcaccactca ggacgcacgc cctttctggc ggctggcatt tttaggcaac
     1081 tcgattaggc agttctgccg atttggcgtg gggctcggca atctacattg gaaccggtag
     1141 taggttagcg gccacatcag agtaggaggt gaaagtccta tatcccctgg tcctggacca
     1201 gggagcctcc agaagccgcg tgggggcaca gcgggctggc gcctcgccag cctggttgga
     1261 caacggtcag gtagtacgaa aggagatgaa gtatgcgaca agtagcgcta cgatcgagta
     1321 cgcggcaatt aatactcgtc gcgatcgcgt gcatgatcgc tctggtcgta gcgggcggcg
     1381 tcttcggcct gtccaggccg gcagacgcct cttcgcacag ggaagcgccg ttcatcggcg
     1441 gtgatccgga ggcggacgct accgacgtct acgcgttccg cagccccgac aagccaaaca
     1501 cggtcacgct catagcgaac tacgtgccgc tggaggtggc ggcggcgggc ccgaacttct
     1561 acaagttcgg cgaggatgtc ctttacgaga tcaacgtcga caacgatggg gacgccaagg
     1621 acgacatcag cttccagctc gacttcagga accgcatcaa caacggcgac accttcctcc
     1681 acaacaccgg ccagatcacc tcgctcgatg atccggatct gaaccaggaa cagttctatt
     1741 ccctgtcggt gctcaaggga gacgatcaca acgatccgtc ccgggagggg aaagtcatcg
     1801 cccagaacct gcaggtcgca ccggccaacg tggggcccaa gtcttatcct cagggctaca
     1861 gcaaggtggc gaaccaggcc atctatacgc tcgataacgg cgtcaaggtc ttcgccggac
     1921 cgcgtgacga tcccttcttc gccgacctcg gaggcatatt cgacctcctg caaggtattg
     1981 aagggaggga ttacctcaag gacttgaacg tgcacaccat tgccgtccag gtgccgatca
     2041 gcatgctcaa aggccctgac gactccatca tcggtgtgag gacgacgtct taccgccaga
     2101 gcacgagcgt cttgcggccg gttggcgatc ccgactcgca gctcgacgat ccgcgcgcta
     2161 ccagcggccc ctgggtgcag ctctctcgcc tggacatgcc gctggtgaac gagctggtca
     2221 taccgctcaa ggacaagaac cgcttcaacg gctcccaacc gcgcttcgac ggccagttcc
     2281 tcacgtacgt gcgcaacccg gagcctgccg gcctcataga gaatatcctt ggcatcgacg
     2341 tgccacccaa cccgcgcgac gacatcgcca cgatcttctt gactggtata caaggcctga
     2401 acaaaccgac gggtgtaaag ccttcgtctc agttgcggct gaacatggac gtgcagccca
     2461 aggcctcgcc gatccggttg ggtgtcctgg gtggagacaa cgcgggcttc ccgaacgggc
     2521 ggcgtctgac ggacgacgtt gtcgacatag agctcca
//
LOCUS       DASTUV010000480         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_407227, whole genome shotgun sequence.
ACCESSION   DASTUV010000480 DASTUV010000000
VERSION     DASTUV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407227"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VFT03_09730"
     CDS             complement(<1..1272)
                     /locus_tag="VFT03_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU4848469.1"
                     /translation="MTTRSTLRAGDAAVEVLVRAGVRRFYTVPGESFLEVIDAVEEHP
                     ALSLVSTRHESGASFMAEADAKLTGVPAVAMATRGVGASNLAIGVHTAMQDSTPMVVL
                     LGHVERHFLGREAFQEVDLAAFYAPISKWAVTVHSADRVAEYVERGLRIATSGRPGPV
                     MLALPADILGEGVTVQDARAGAPRPVRPLPSAEEVQSVARKLSEAQRPVMIVGGGAGH
                     ARDELLALAEAFDVGVYAAFRRQDVFPNEHPLYLGHLTLATPPDTLKALEEADVVLVV
                     GSRLDEVTTQSYELPRRDQAVIQVDVDPGEIGATIPVEHGFVADTRAALTSVVAQAPS
                     TKLARDWSTAHRAYLDSSSIPASRTADGIDPAQVIASFREVLPEDVLFANDAGNFSAF
                     LHRYWRYNHPHTQLAPANGAMGYGVPAAVAAK"
     gene            1749..>2272
                     /locus_tag="VFT03_09735"
     CDS             1749..>2272
                     /locus_tag="VFT03_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU4848470.1"
                     /translation="MTDYFTDTEEVLDGPPPFVAGERLAPGYEVIAHMIRSNNLDVYD
                     AWSEERACRVIAKTPRPDRLEDRRTVRALMREGRLLKKFTHPHIVRAYEVSKEPQPVV
                     ILETLTGATLAYLIDTRPRRLAISEIVHLGLHLCSALHYMHRQGVLHLDLKPSNIISE
                     SQLAKIIDLSIARSP"
BASE COUNT          412 a          749 c          717 g          394 t
ORIGIN      
        1 cttcgccgct actgcggccg gtactccgta gcccatcgcg ccgttggctg gggcgagctg
       61 cgtatgcggg tggttgtagc gccagtagcg gtgcaggaag gcagagaagt tgcccgcgtc
      121 gttggcgaag agtacatctt ctggcaacac ctccctgaag gaggctatga cctgggcggg
      181 gtcgatcccg tccgcggtcc ggctggcggg gatcgagctg gagtccaggt aggcccggtg
      241 ggccgtgctc cagtcgcgcg cgagcttggt ggatggagcc tgggctacga cggaggtcag
      301 agcggcgcga gtatctgcga cgaagccgtg ctcgacggga atcgtagccc cgatctcgcc
      361 gggatccaca tcaacctgga tcacggcctg atccctgcgg gggagctcat agctctgggt
      421 tgtcacctcg tcgaggcggc tccccaccac gagcaccacg tcagcctcct ccagcgcctt
      481 caaggtatcg ggtggcgtgg ccagggtgag gtgccccagg tacagggggt gctcgttcgg
      541 gaagacgtcc tgcctgcgga acgccgcgta gacgccgaca tcgaaggcct cggcgagggc
      601 gaggagctcg tcgcgcgcgt ggcccgcccc gccgccgact atcatcactg gacgttgcgc
      661 ctcgctcagc ttgcgggcga cggactgcac ttcttcggca gagggtaggg ggcgcaccgg
      721 acggggtgcg ccagcccgtg cgtcctgaac ggtgacgcct tcacccagga tgtcggccgg
      781 cagggcgagc atcaccggac cagggcggcc ggaggtggcg atgcgcaggc ccctctccac
      841 atactccgcc acccgatcgg cgctgtggac cgtcactgcc cacttgctga tcggggcgta
      901 gaaagccgcc aggtccacct cctgaaacgc ttccctgccc agaaaatgcc tctcgacgtg
      961 ccccagcaag acgaccatgg gcgtggagtc ctgcatcgct gtgtgcaccc cgattgccag
     1021 gtttgaggcg cccacgccgc gggtagccat cgccacggct ggcaccccgg tgagcttggc
     1081 gtcggcctcg gccataaaag aggcgcctga ctcgtgcctg gtggagacca gcgagagagc
     1141 gggatgctct tccacggcgt ctatgacctc gaggaagctc tcgccgggga cggtgtagaa
     1201 gcgcctgacg cctgcccgca ccagtacctc caccgcagcg tctccggctc tcagcgtgga
     1261 gcgagtagtc atcgcatcct cctcttggta gcgttggctt cgggcgtgtt cgttaccaat
     1321 cgattaccaa aacgtcacaa gtccctatgc ttgccgattt tccacactat gcagatgaat
     1381 ccttacccgc cgcatctata acaataacga cctgaacttg cgcacctcgt tcgtgatgtt
     1441 ttagaccaag tcccaccaca gggagtccag atcaccgcgg ttgggcttcg cgcagaactc
     1501 tcgcccgaag ttgaacatca cgattggagc ggaaagtggg gtcagaaagt tttgtccatc
     1561 gtcagagcgg catcctcacg catgtcctcg aggacatggg cgtacctcct gagcgtcatc
     1621 gccgcgtcgg agtgtcctaa ccccgcagct tcgagttgcc ggttgcctat atcgacccgt
     1681 ctgcggtact ctggccgaag acgtaggagg catggaggaa cggaggagga agcgacaacc
     1741 cccgagctat gaccgactac ttcaccgaca cggaagaggt cctcgacggt cctcccccct
     1801 tcgtcgcagg ggagcgtctc gcgcccggct acgaggtaat agcccacatg attaggagca
     1861 acaatctaga cgtctacgac gcgtggagcg aggagcgcgc gtgccgcgtc atcgccaaga
     1921 cgccgcgccc cgatcgtctc gaggatcgga ggacggtgcg cgccctgatg cgggaggggc
     1981 gcctgctcaa gaagttcacc cacccgcaca tcgtgcgcgc ctacgaggta tcgaaggaac
     2041 cgcaacccgt cgtcatcctg gagacgctca ccggggcgac gctcgcgtac ctcatcgaca
     2101 cacgcccccg gaggctcgcg atctccgaaa tagtgcacct cgggctccac ctgtgctcgg
     2161 cgctgcacta catgcaccgc cagggtgtgc tccacctcga cctcaagccc tccaacataa
     2221 tctccgagag ccagctcgcg aagatcatcg acctcagcat cgcgaggtcc cc
//
LOCUS       DASTUV010000481         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_409592, whole genome shotgun sequence.
ACCESSION   DASTUV010000481 DASTUV010000000
VERSION     DASTUV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409592"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFT03_09740"
     CDS             <1..276
                     /locus_tag="VFT03_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848471.1"
                     /translation="TADQEYAYLKVPPGTELRSCVGLVLAGMAARAKVGVGGLEEAVE
                     LLQGFHSDDAPSRFRFSLGHGGVLAEVEEPADEPEGFRWRTVVELVS"
     gene            273..1046
                     /locus_tag="VFT03_09745"
     CDS             273..1046
                     /locus_tag="VFT03_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEU4848472.1"
                     /translation="MSRRYRRPDKARTRLLLERYHREGDENARERVIQEQLPLAEFLA
                     RKFAGRGEPVDDLVQVASVGLIKAVDRFDVDRNIEFSTYATPNILGEIKRYFRDKGWA
                     MRVPRGLQELRQAAKESIRDETVRTGRSPTIQELAETLNSDVESVAEALTLGWAYNTA
                     SLDAPVSQDEPDGDTIMDLQADDRSPIDGLEDRILLQKAIAHLKDQQQQILNLRFNEG
                     KTQTEIADHIGVSQMHVSRLLRRAMEDLRHELEELESNG"
     gene            1066..>2277
                     /locus_tag="VFT03_09750"
     CDS             1066..>2277
                     /locus_tag="VFT03_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEU4848473.1"
                     /translation="MGQIKSSEIRQRFLDFFVERDHRLYKSSSLIPHNDPTVLLTTAG
                     MQQFINYFTGQETPPAPRAVSVQKCFRTADIEEVGDPSHLTFFEMLGNFSFGDYFKKE
                     AIEWAWAFLTDDLGIAPERLWITIFEGDEDAPEDLEAKEHWMNVGVPEHKIFGLPKSE
                     NWWGPPGDSGPCGPCSEIYYDYGEEYANGDPAEDPKYGPGGDEGDPRFLEIWNLVFNQ
                     YEQLKDGTLKPLAKPGIDTGLGLERTTAALQSVLSVYETDAYTPIIEKITEYTGIEVG
                     ASDATDRAFYILADHARAMAFLIADGVRPGNQHREYVLRRIIRRATREGYGRLGLSAE
                     QIASLAEVVVDYMGDFYEELRAAREDIRRIVAGEAARFIEIYHSGMELLEAEIGRLAG
                     GNLPGEVAFLLH"
BASE COUNT          460 a          730 c          739 g          348 t
ORIGIN      
        1 accgccgacc aggagtacgc ttacctcaag gttccgcccg gcacggaact gagatcgtgc
       61 gtgggcctcg tgctggccgg gatggcggcg cgggccaagg tcggcgttgg ggggttggag
      121 gaggccgtgg agctcttgca gggcttccac tcggatgacg cgccttctcg cttccgcttc
      181 tcgctggggc atggcggggt cctcgccgag gtcgaagagc cggccgatga gcccgagggc
      241 ttcagatggc gcacggtggt agagctggtt tcgtgagccg acgctacaga cgaccagaca
      301 aagcgcgaac caggctgctg ctagagcgtt accaccgcga aggcgatgaa aacgcccgcg
      361 agcgggtgat ccaggagcag ctcccgctcg ccgagttcct ggcccgcaag ttcgcgggcc
      421 gcggcgagcc cgtcgacgac ctggtccagg tggcctcggt tgggctgatc aaagccgtag
      481 accgcttcga cgtggaccgg aacatcgagt tctcgaccta cgctacgccg aacattctcg
      541 gggagatcaa gcgctacttc cgcgacaagg gctgggccat gcgcgtcccg cgcggcctgc
      601 aggagctcag gcaggcggct aaggagtcga tccgggacga gaccgtcagg acgggccgct
      661 cgcccaccat ccaggagctc gccgagaccc tgaactcgga cgtggagagc gtcgccgagg
      721 ccctcaccct gggctgggcc tacaacaccg cgagcctcga cgcccccgtg agccaggacg
      781 agcccgacgg tgacaccatc atggacctcc aggccgatga ccgctccccg atagacgggc
      841 ttgaggacag gatcctgctc cagaaagcca tcgcccacct gaaagaccag cagcagcaga
      901 tactcaacct ccgcttcaac gagggcaaga cccagacgga gatcgccgac cacatcgggg
      961 tgagccagat gcacgtctcc cgcctcctgc ggcgcgccat ggaggacctg cgccacgagc
     1021 tggaagagct ggaaagcaat ggctaaaaaa gtacaataat ctcaaatggg acagataaag
     1081 agcagcgaga tacgccagag attcctggac ttttttgtag agagggacca ccgtctctac
     1141 aagagttcct ccctgattcc acacaacgac ccgaccgtgc tcctcaccac cgcgggcatg
     1201 cagcagttca taaactactt cacgggccag gagaccccgc ccgctccacg cgcggtcagc
     1261 gtccagaagt gcttccgcac cgcggatatc gaggaggtcg gcgacccgag ccacctcacc
     1321 ttcttcgaga tgctcggcaa cttctccttc ggggactact tcaagaagga ggccatcgag
     1381 tgggcctggg cgttcctcac cgacgacctc ggcatagcgc ccgagcggct ctggatcacg
     1441 atcttcgagg gcgacgagga cgcacccgaa gacctcgagg cgaaagagca ctggatgaac
     1501 gtcggagtgc ccgagcacaa gatctttggc ctcccgaaga gcgagaactg gtgggggccg
     1561 cccggtgatt ctggcccctg cggcccgtgc tccgagatct actacgacta cggcgaggag
     1621 tacgccaacg gcgaccccgc cgaagacccg aagtacggtc ccggcggcga cgaaggcgac
     1681 cctcgcttcc tcgagatctg gaacctcgtc ttcaaccagt acgagcagct caaggacggc
     1741 accctcaagc cgctggcgaa gcccggcata gacaccggcc ttggcctgga gcgcacgaca
     1801 gccgcgctcc agagcgtcct ctccgtctac gagacggacg cttacacccc catcatcgag
     1861 aagataaccg agtacaccgg catcgaggtg ggcgcctcag acgcgaccga ccgggccttc
     1921 tacatcctcg ccgaccacgc gagggcgatg gcgttcctca tcgcagacgg cgtcagaccc
     1981 ggcaaccagc accgcgagta cgtccttcgc cgcataatac gccgagcgac gcgcgagggg
     2041 tatgggcggc tagggttgag cgcggagcag atcgcgtcgc ttgcggaggt agtggtggac
     2101 tacatggggg acttctacga ggagttgcga gctgcgcggg aggatattag gcgcattgtg
     2161 gcgggtgagg cggcgcgatt catcgagatc taccactccg ggatggagtt gttggaggcc
     2221 gagatcgggc gtctggccgg agggaacctg ccgggcgagg ttgcgttctt gttgcac
//
LOCUS       DASTUV010000482         4789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1265533, whole genome shotgun sequence.
ACCESSION   DASTUV010000482 DASTUV010000000
VERSION     DASTUV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265533"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(406..1494)
                     /locus_tag="VFT03_09755"
     CDS             complement(406..1494)
                     /locus_tag="VFT03_09755"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU4848474.1"
                     /translation="MVETPHLSIDGAKMKKNIEKMAAIAARNGVALRPHVKTHKIPTL
                     AWAQVEAGAAGITVAKVSEAEVMADAGLEDIFIAYPLVTASKIRRAVELRRKLQKLIV
                     GVDSLEGARRLSAAAQDDVLEVRLEVDTGLRRTGVAYDEAVELALEIDTFDNLDLSGI
                     YTYRGAVFGGAPTLDRKNAGLEEGDLMVSLAGRMREQGISIRDVSLGSTPTAEYAAEV
                     EGVTEIRPGTYVFYDRMQARLGACSLEECAGTVVCTVISRPHEDLAVIDGGSKTFATD
                     VQPGIEPLNLEGFGHVVGYPGVTLERLTEEHGMLAVTGEHDLKVGDTLQIIPNHICST
                     VNLHDAVYLIGEGGIVEEVRVEARGKVQ"
     gene            complement(1528..3123)
                     /locus_tag="VFT03_09760"
     CDS             complement(1528..3123)
                     /locus_tag="VFT03_09760"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU4848475.1"
                     /translation="MTFDVLIKGARVFPGDGPALDADVGIAGDRIAAFEHSLPATNTA
                     EVIDAAGMMLCPGFIDMHAHTALEPFRDPRLEPKVAQGFTTEVINPDGLAPAPVEEDS
                     REERQAYLRPLEGPGPERWGWTTFSEYLEALEGTRPATTLVPSIGHNAVRDRVMGGAD
                     RRPEREEIRAMREEVRLGLEAGARTFSFGLIYMPGVFSETGELVELAEEAAFFGAPLV
                     PHVRTEADGVLGAVGEMIEVSRRSGAPLHLSHLKVIGNPELVESLLALIDLASADVDI
                     TFDQYPYGAGSTVLSSLLPPWAHAGGPSDALRRLEDSRQREAMARDVEKGLPDWDNSY
                     GACGPANIVIVHAPVAQAGVVGKSLAEIGEERGCDPFVAALDLLKESRLDVAMINHYA
                     TEEAVRTVFRHPLQLVGSDGIFGDRPHPRLYGTAARALGRYALKEKLIPIEEAVARLT
                     SRAAERIGLTDRGRIREGLRADLVLLDPERFIDTATYDDPKQVPTGVERVFVAGRTVW
                     AGSGHTGELPGGVVREPLTRATD"
     gene            complement(3148..4329)
                     /gene="thrC"
                     /locus_tag="VFT03_09765"
     CDS             complement(3148..4329)
                     /gene="thrC"
                     /locus_tag="VFT03_09765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU4848476.1"
                     /translation="MPDYCWRCGSPLEVDLDYDRVRLAALFEGGVTNVWKYRALYPLS
                     SHRHELELSLGEGGTPLVLAAKVGRKIGVEKTWFKCDHLNPSGSFKDRSAAVGVAWAL
                     EQGYPGVICASSGNAAGASATYAARAGLPVYLVVSSRTPKSKLGAAAAHGARVFRVTG
                     DFSRAFAAAREAAVEVGLANLTTTYVNCYACEGNKSVAYELYEQMDVVPDWVVVPVGS
                     GPLLFGVWKGFEELRQFGLVDRTPRLMAVQPDGCAPIARAFETGTEVVPWSRVDTVVS
                     GLDDPLQGYEHDGTLTLHTVRESRGVVLAVSDEDTLEAGRLLAEDEGIFVEPAAATSV
                     AGASVARRLGHIDESASVVCLLTGHGLKYQAPRSEPEPESAADGAELVRAIRRDVGDC
                     G"
BASE COUNT          843 a         1641 c         1410 g          895 t
ORIGIN      
        1 cgcggcctcc agaatcgttt gcaggttctc gagcgtctgg cgaacctggt cagcgaaacc
       61 atccggcata ttgcctgtcg cgggatctac tggtccctgg cccgagacga acacgaagcc
      121 gcctgagacc acggcgtggg agtagggacc cgcgggagcg ggtcctccct cagccatcac
      181 ggttctcctc atatctcctc ctttgtagaa gtcatgcctc gctaggtcgt ctgccctgcc
      241 ctgctcgccc cacgttctct ggtgaactct ttggcgagct cctcccggaa cgactccgtg
      301 gccaggtaga ccgagacgaa gctgatgagg cccgccacca gcaacaccgc cggcgaacac
      361 ggcagccatg gtagcctaga gagaccttga tgtaacaaac gttgcttact gcaccttccc
      421 acgagcctcc acccgcacct cctccacgat gccgccctcg ccgatcagat aaaccgcgtc
      481 gtggaggttt accgtgctac agatgtggtt cgggatgatc tgaagcgtgt cgcccacctt
      541 cagatcgtgc tcgccggtga cggcgagcat cccgtgctcc tcggtgagcc tctcgagcgt
      601 gactccggga tatcccacca cgtgaccgaa cccctccagg ttcagaggtt cgatgcccgg
      661 ctgcacgtcg gtggcgaagg tcttgcttcc cccgtcgatg acggccagat cctcgtgcgg
      721 cctgctgatc accgtgcaca ccaccgtccc ggcgcattct tcgagcgagc aggcgccgag
      781 tcgagcctgc atcctgtcgt agaagacgta ggtgcctggc cggatctccg tcaccccctc
      841 cacctcggcc gcgtactcgg cggtcggggt ggagccgagg ctgacatccc gtatgcttat
      901 cccctgctcc ctcatacggc cggctagaga gaccatgaga tcaccctcct ccagacccgc
      961 gttcttgcgg tcgagggtgg gcgcgccgcc gaacacggca ccacgatatg tatagattcc
     1021 gctcaggtcg aggttgtcga aggtgtcgat ctccagggca agctcgacgg cttcgtcata
     1081 cgcgacaccg gtcctcctca gcccggtgtc tacctccagc ctcacctcca gaacatcatc
     1141 ttgcgcagcc gccgagagcc tgcgcgctcc ctccaagctg tccaccccga cgatgagttt
     1201 ctgcagcttc cgcctcagct ctaccgcgcg acggatcttg gaggccgtca ccagcgggta
     1261 ggcgatgaag atgtcctcga gtccggcgtc ggccatgacc tccgcttcgg agaccttcgc
     1321 caccgtgatg cccgcggccc cagcttcgac ctgagcccag gccagcgtgg gtatcttgtg
     1381 cgtcttcacg tgcggcctca gcgcgacgcc gttccgagcg gcgatcgcgg ccatcttctc
     1441 gatatttttc ttcatcttcg cgccgtcgat cgacaggtgc ggcgtctcca ccatctcatc
     1501 cccctggatc tctgagtttt gcgttcacta atccgttgcc ctcgtgagcg gctcgcgaac
     1561 gacaccgcca ggtaactctc cggtatgccc gctcccagcc cacaccgtgc ggccggccac
     1621 gaacacccgc tcgaccccgg tgggaacttg cttcggatcg tcgtaggttg ccgtgtcgat
     1681 gaaccgttcg gggtctagta gcaccaggtc ggcgcgcagg ccttctctga tacgtccccg
     1741 atccgtaagg ccgatgcgct cggccgcgcg gctggtcaga cgggcgaccg cttcctcgat
     1801 gggtatcagc ttctccttga gcgcgtatct ccccagcgcc cgggcggccg tgccgtacag
     1861 ccgggggtga ggtctgtccc cgaagatgcc gtccgagccg acgagctgta gcggatggcg
     1921 gaagacggtc cgcaccgctt cctcggtggc gtagtggttg atcatcgcca cgtcgagtct
     1981 ggactccttc aagagatcga gggcggcgac gaaaggatcg cagccacgct cctcgcctat
     2041 ctcggcgagc gacttgccga cgaccccggc ctgcgccacc ggtgcgtgaa cgatcacgat
     2101 gttcgcgggc ccgcacgcgc catacgagtt gtcccagtcg ggcaaccctt tctcaacgtc
     2161 gcgggccatc gcctcgcgct gcctggaatc ctccaggcgc ctgagcgcgt cggaggggcc
     2221 gcccgcgtgc gcccaggggg ggagcagcga agacagcacg gtgctgcccg cgccgtaagg
     2281 gtactggtcg aaggtgatgt ccacatcggc gctggccagg tcgatgagcg cgagcaacga
     2341 ctccaccagc tcggggttgc cgataacctt gaggtgcgag aggtgcaggg gggcgcccga
     2401 acgtcgagag acctcgatca tctctcccac ggcacctagc acaccatcgg cttcggtgcg
     2461 cacgtgagga accagcggtg cgccgaaaaa cgctgcttct tcggccagct cgacgagctc
     2521 ccccgtctcc gagaacacgc cgggcatgta aatcaagccg aaggaaaatg tacgagctcc
     2581 tgcctcgagg cccaacctca cttcttcccg catggcgcga atctcttccc gttcgggccg
     2641 ccggtcggcg ccacccatga cgcgatcgcg cactgcgtta tgccctatgg agggaacgag
     2701 ggtcgtcgcg ggccgcgttc cttcgagggc ttccaggtac tccgagaacg tcgtccaccc
     2761 ccacctctcc gggcctgggc cttcgagcgg tcgcaggtag gcctggcgct cttcgcggct
     2821 atcctcttcg actggagccg gcgcgagacc gtctgggttg atcacctccg tggtgaaacc
     2881 ctgcgccacc ttcggctcga ggcgcggatc gcgaaacggc tccagcgccg tgtgggcgtg
     2941 catgtctatg aagccggggc agagcatcat gccggcggca tcgattacct ccgctgtgtt
     3001 cgtcgcgggt aacgagtgct cgaacgccgc gatacgatcg ccggcgatgc cgacgtcggc
     3061 gtctagcgcg ggaccatcac cggggaaaac tctggcaccc ttgatcagaa cgtcgaaggt
     3121 cacatcgctc cttcttgtac ccgagggcta gccacaatct ccgacatcgc gccttatcgc
     3181 tcgtactaac tcggctccgt cagcagcgga ctcaggctcg ggctccgacc tgggggcctg
     3241 atacttgaga ccatggccgg tcagcaggca gacgaccgac gcggactcat cgatgtgccc
     3301 aagccgtctt gcaaccgaag ccccagctac ggaagtcgca gctgcaggct ccacgaatat
     3361 gccttcatcc tcggcaagaa ggcgaccggc ctcgagcgta tcctcgtccg aaacggcgag
     3421 gacgaccccc ctcgattcgc gcacagtgtg caaggtcagg gttccgtcgt gctcgtatcc
     3481 ttgcagggga tcgtcgagcc cggacacaac ggtgtctacc cgtgaccatg gcacaacttc
     3541 ggttccagtc tcgaatgctc gagctatggg ggcacatccg tccggttgga cggccatgag
     3601 tcggggcgtc cggtcaacga ggccaaactg ccgcagttcc tcgaaacctt tccacacccc
     3661 gaagagtagc ggtccggatc ccacggggac cacgacccaa tccggaacca cgtccatctg
     3721 ctcgtacagc tcgtaggcga ctgacttgtt gccctcacac gcgtagcagt tgacgtaggt
     3781 ggtagtcagg ttcgccagac cgacttccac ggccgcctct cgagccgccg cgaaggcgcg
     3841 gctgaaatcc cccgtgactc ggaacactcg agctccgtgg gcggcggccg cacccagttt
     3901 cgacttcggc gttctggagg aaacgacaag gtagacgggt agtccggcgc gcgccgcgta
     3961 ggttgcggat gcacccgccg cattcccgct cgaggcacag attaccccag ggtacccttg
     4021 ctccagcgcc cacgcgacac ctaccgccgc acttctgtct ttgaacgaac cggacgggtt
     4081 cagatgatcg cacttgaacc aggtcttctc cacgccgatc ttccgaccca cctttgcggc
     4141 gagcaccagc ggagtccccc cttcgcccag gctgagttcc aactcatgac gatgggaaga
     4201 aaggggatag agcgccctgt acttccacac gttggtgaca ccaccctcga acaatgcagc
     4261 cagtcgaact ctgtcgtagt cgaggtcgac ctcaagcggt gagccgcagc gccaacagta
     4321 gtcgggcatg gggctgagtt tgctggcatc acaagccgta caatgcactc gccacctggc
     4381 acgagacgca tcttgcctgc tactaccgag acgccaatct ataccgcgtc tctgtgttga
     4441 cacccccgtt cctcaccttg ccccatcgaa gcactttacg atacaaactt cttcccattt
     4501 ttcggcgcgc tgaaacctgc tctgacaatc gaggcccatg caccgcccct cgtggtgccc
     4561 cagtcaggct gcactatctg accgacgagt gcagccgacc cctcacgcgc gaggaacgcc
     4621 acggtggtcg tggtgtcctc cgggcgcccg atgcgtccaa cgagcgtcag tgtgacgggc
     4681 ggtgtaggcc gcgtgatctg cggaggactt tgtggcaggc atggtctccg aaacggtcaa
     4741 ttctcacctc gataaagatc tggagaagag tttggcttcg taagatggc
//
LOCUS       DASTUV010000483         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_980129, whole genome shotgun sequence.
ACCESSION   DASTUV010000483 DASTUV010000000
VERSION     DASTUV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980129"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFT03_09770"
     CDS             complement(<1..810)
                     /locus_tag="VFT03_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564561.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU4848477.1"
                     /translation="MSYGETNEAWIVGAVRTPIGRHGGALSSVRPDDLGALVLEALME
                     RTRVSPEEVEDVYFGCANQAGEDNRNVARMSLLLAGFPESVSGATVNRLCGSGLEAVA
                     NAARAVMLGEAEVYIGGGVESMSRAPWAMPKPARAFPTGHTTIYDTTLGWRFVNPRME
                     KMGHTDSLGMTAENLAEERYHLSSEEQRPEELARTYDISREKQDRFALGSHRKALAAI
                     DEKRFREEIVAVTVKSGKGATTVVEADEGPRRETTLEKLGKLKAAFKEDGSV"
     gene            complement(853..1641)
                     /locus_tag="VFT03_09775"
     CDS             complement(853..1641)
                     /locus_tag="VFT03_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HEU4848478.1"
                     /translation="MSVRASAAEGRYTTDEMMTVAASRELGDGTVCFVGIGLPSEAAN
                     LARATHAPGCFLIYESGTLGAKPDVLPLSIGDGVLAEHADSVVPVPEVFNYWLQAGRV
                     DVGFLGAAQIDRYGNINTTVVGPYDDPKVRLPGAGGAPEIAASAKEVIIMLRHNRRAF
                     VEELSFVTSVGHGKGGDSRKDLGYAGAGPTVVITDLGVLRPDPETKELTLVALHPGAT
                     VEQAREATAWKLRVTEDPGTTDPPTDKELAVLRDLRARTEAARF"
     gene            complement(1638..2498)
                     /locus_tag="VFT03_09780"
     CDS             complement(1638..2498)
                     /locus_tag="VFT03_09780"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU4848479.1"
                     /translation="MAELKELREAVAGLVQSGDSVALEGFTHLIPTAAGHEIIRQERR
                     DLTLIRMTPDLIYDQMIGMGCARKLVFSWGGNPGVGSLHRFRDAVEHGWPVPLEIEEH
                     SHAGMANRYVSGASNLPFAVLRGYVGTDLPKVTPTIVPITCPFTGEKLMAVPALRPDV
                     AVIHAQQADRRGNVAIWGIVGIQKEAVMSATKVIVTVEEIVDELEPHPFQVVLPSVVI
                     DAISVVPFGAHPSYADGYYARDNAFYEAWDPIGRDREAFREWMERHVLGTEEWGEYLE
                     TLREEELIRE"
     gene            2754..>3220
                     /locus_tag="VFT03_09785"
     CDS             2754..>3220
                     /locus_tag="VFT03_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848480.1"
                     /translation="MEHWHGFETARNVAQIHETTIADTASDLSAHFVTVPARSLGGGS
                     YLFQGSEGPLEEIGLLRIADVPFGGVARSLAALLVSEGVEDFLRRFDQGFEEETRHAS
                     TQLPKPFEFAEYLTFARVVPFEWPSLAADSLGNILTAQGQGEAGYMYHEETATP"
BASE COUNT          535 a         1100 c          971 g          614 t
ORIGIN      
        1 gacgctgccg tcttccttga aagcagcctt gagcttgccc agcttctcga gcgtcgtctc
       61 gcgcctgggg ccctcgtctg cctcgacgac cgtagtagcg cctttgccgc tcttcaccgt
      121 aaccgccacg atctcctcgc ggaacctctt ctcatcgatg gcggcgagag ccttccgatg
      181 gctacctagc gcgaaacggt cctgcttctc gcgcgagatg tcgtaggttc tggccagctc
      241 ttcaggcctc tgctcctccg agctcaggtg gtagcgctcc tcggctaggt tctcggcggt
      301 catgcctaac gagtcggtgt gacccatctt ctccatcctg gggttgacaa agcgccatcc
      361 caaagtggtg tcatagatgg tggtgtgccc tgtgggaaag gccctcgcgg gtttgggcat
      421 agcccaggga gcgcggctca tggactccac cccgccccct atgtagacct cagcctctcc
      481 tagcataacg gctcttgcgg cgttagccac agcctcaagc ccagagccgc acagcctgtt
      541 gacggtagca cctgagacgc tctctgggaa gcccgcgagc agtagcgaca tacgtgccac
      601 gttgcggtta tcctcgcctg cctggttggc gcagccgaag tagacgtcct cgacctcttc
      661 agggctgaca cgggtgcgct ccatgagggc ttctaggact agcgcgccga gatcgtcggg
      721 acgcacggag ctaagagcgc caccgtggcg gcctatgggg gtacggaccg cgccgacgat
      781 ccaggcctcg ttcgtttctc cgtaagacat gggattcatg cgctcctttc ggtcgcgggt
      841 ctcatcccgt ccctaaaacc tggccgcctc ggtcctcgcg cgcaggtcgc gcaacacggc
      901 gagttccttg tccgtcggcg ggtcggtggt tcctggatct tctgtgaccc gcaacttcca
      961 agccgtcgcc tcccgggcct gctccacggt cgcgcccggg tgcagggcga ccagggtcaa
     1021 ctccttcgtc tcggggtcgg gtctgaggac accgaggtcg gtgatcacga ccgtcggccc
     1081 ggcacccgcg tagccgagat ccttgcggga gtcaccaccc tttccgtgcc cgaccgaggt
     1141 gacgaatgag agctcttcga cgaaagcgcg cctgttgtgg cggagcatga taatcacctc
     1201 tttcgcggag gcggcgatct cgggcgcccc gccagccccc ggcaggcgca ccttcgggtc
     1261 atcgtaggga ccgacgacgg tggtgttgat gttcccgtag cggtctatct gggccgcccc
     1321 gaggaagccg acgtccactc ttcccgcctg cagccagtag ttgaagacct ccggtaccgg
     1381 gaccaccgaa tcggcgtgct cggcgagcac cccgtctccg atggagagtg gcagcacgtc
     1441 aggcttggcg ccaagggttc ctgactcgta gatcaggaag cacccgggcg cgtgcgtcgc
     1501 acgggccagg ttcgcagcct cgctcgggag cccgatcccg acgaagcaga ccgttccgtc
     1561 cccgagttcg cgcgaggcgg ctaccgtcat catctcgtct gtcgtgtatc tgccctccgc
     1621 ggcagacgct cggacgctca ctctctgatc aactcctctt ctcgaagggt ctccaggtac
     1681 tcgccccact cttcagtgcc gaggacgtgg cgctccatcc actcccggaa cgcctcgcgg
     1741 tcgcgcccga tcgggtccca cgcctcgtag aaggcgttgt cgcgggcgta gtagccgtcg
     1801 gcgtaagagg ggtgggctcc gaagggcacc accgagatcg catctatgac cacggacggc
     1861 aaaactacct ggaaggggtg aggctcgagc tcatccacta tctcctcgac cgtcacgatg
     1921 accttcgtgg cggacatcac cgcctccttc tggatgccca cgatacccca gatcgccacg
     1981 ttcccccggc ggtcagcctg ctgggcgtgg atcaccgcca cgtccggcct caaggccggc
     2041 accgccatca gcttctcccc ggtgaacggg cacgtgatgg gcactatcgt cggggttacc
     2101 ttgggcaggt ccgtgccgac gtagccgcgc agcactgcga acgggaggtt ggaggcgccg
     2161 gagacgtagc ggttcgccat gcctgcgtgg ctgtgctcct cgatttcgag gggcacgggc
     2221 caaccgtgct ctactgcatc cctgaagcgg tggagggagc cgacgccggg gttgccgccc
     2281 cacgagaaga cgagcttcct tgcacagccc atcccgatca tctggtcgta gatcaggtcc
     2341 ggcgtcatcc ggatcagggt gaggtcccgc ctctcctggc ggatgatctc gtgcccagcg
     2401 gcggtcggta tgaggtgcgt aaacccttcc agggcgaccg agtcaccact ctgcaccagc
     2461 ccagcgaccg cctcccgtag ctcttttaac tcagccacat cgaatcctta cccacttcct
     2521 cgtctcctcc tatgtcctcg aatgtggcgc atgatcgggg agcgcgaaga ggccgggtcc
     2581 cgcctctccg caggatcgca cattctcacc agacgcccca actcctgttg ctcctccttg
     2641 ccccacaagc tattctggtc gctatagcgc aatatatcca ataccaaatg agcgttccgg
     2701 ataaaccatc acgataacgt gcgcggcacg cggataggag atactcaccg ggtatggagc
     2761 actggcacgg tttcgaaacg gcccgcaacg tggcgcagat acatgagact acgatcgcgg
     2821 acactgcatc agatctctct gcccactttg tcacggtacc ggcccgctcg ctcgggggcg
     2881 gctcctatct cttccagggc tccgaaggcc ccctggaaga gatagggctg ctgcgcatcg
     2941 cggacgttcc gttcggcggc gtggcccgca gcctggccgc gttgctggtc tccgagggcg
     3001 tcgaggattt cctccgccgg ttcgaccagg gcttcgagga agaaaccagg cacgcgagca
     3061 cccagctgcc caaaccattc gagttcgccg agtacctcac gttcgccaga gtcgttccgt
     3121 tcgagtggcc ctcgctcgca gccgattctc tcgggaacat cctgacggcc cagggccagg
     3181 gcgaagcggg ctacatgtac cacgaagaga cggcaacccc
//
LOCUS       DASTUV010000484         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_907971, whole genome shotgun sequence.
ACCESSION   DASTUV010000484 DASTUV010000000
VERSION     DASTUV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907971"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="cbiE"
                     /locus_tag="VFT03_09790"
     CDS             complement(<1..619)
                     /gene="cbiE"
                     /locus_tag="VFT03_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEU4848481.1"
                     /translation="MSHITVIGLDGRPLSAEAESLLRGAALVVGGRRHLAALAVEGER
                     SAVLEGDLSGALASMERASGPVVVLASGDPGFFGIVRLLGEWFGRASLRVLPAVSYVA
                     LAFARAGISWDDAVTVSAHGRDPRRAVNVCRAHPKVAVLTSPRYGPAELARELEGWER
                     TYFVAEKLGEQDERAFHGDAAAVLDENWDYPNVVLVLDERRVIGTK"
     gene            complement(616..1785)
                     /locus_tag="VFT03_09795"
     CDS             complement(616..1785)
                     /locus_tag="VFT03_09795"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEU4848482.1"
                     /translation="MPPDRKLREPAMPPSMARAKDEKLRTGWTTGTCAAAAAKAAATA
                     LITGEPQGRVEVRLPGKGDKRRVSFEVERCEVGEGWAEAVVIKDAGDDPDVTHGARLT
                     ARVSWKEEPGISLDRGEGVGVVTKPGLGLPVGGPAINDVPRRMISYSLDEVLHAGERG
                     VRVVISVPGGEKMAEKTTNARLGIVGGISILGTTGIVRPFSTAAWAASVVQAIDVMGA
                     QGLDHFVLSTGGLTERAAMRLLPALEEVCFIEVGDFTGQAIKGAVKNGLRRGFFVGMA
                     GKLTKLAAGVMMTHWTRSKVDNELLAEITEEAGGDAQLLEDVKGANTARHAYELWRAA
                     HLERAPYLLCGRVAENLCDYAGGEIEMHAIMVDFDTLEPVGASPGALELTTWSKR"
     gene            complement(1775..>2369)
                     /locus_tag="VFT03_09800"
     CDS             complement(1775..>2369)
                     /locus_tag="VFT03_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HEU4848483.1"
                     /translation="GVRELYERAVAEDLRVARVHSGDPSIYGAVMEQVELCEEIGLEW
                     EIVPGVSSLGAAAAAIGRELTVPEVSQSVILTRRASRTPMPDGEDIRSFAAHGTTMAI
                     FLSAARPRALQEELLEGGYAPDTPCAVVYKASWPDELVIECPLEELADRVREAGFTRQ
                     ALILVGPGLAAGGTRSHLYSPQFSHMFRRAEERADAP"
BASE COUNT          366 a          885 c          740 g          378 t
ORIGIN      
        1 ccttcgtccc gatgacccgt ctctcatcga gaacgaggac cacgttgggg tagtcccagt
       61 tctcgtctaa aaccgccgcc gcgtcgccgt ggaaggcgcg ctcgtcctgc tcgccaagtt
      121 tctcggcgac gaagtaggtt cgctcccatc cctccagctc ccgcgcgagc tcggccggac
      181 cgtatcgcgg ggaggtgagg accgcgacct tgggatgcgc ccggcagacg ttgaccgcgc
      241 ggcgcgggtc gcggccgtgg gcgcttaccg tgacggcgtc atcccaggag ataccggccc
      301 gcgcgaaggc gagcgcgaca taggagacgg cggggaggac gcgcaggctc gcgcgcccga
      361 accactcccc gagcaaacgc acgatgccga agaatccagg atccccggaa gccagcacca
      421 cgaccggccc actggcccgc tccatgctcg cgagcgcccc ggagaggtct ccctccagga
      481 cggcggaccg ctctccctcg acggcaagcg ccgccaggtg ccgtctcccc ccgaccacga
      541 gcgcggcccc ccgaagcagg ctttccgcct ccgcgctcag cggacggccg tcgagcccga
      601 tgacggttat gtggctcatc gcttgctcca ggtcgtgagc tccagggcgc cggggctcgc
      661 gccaacaggc tccagggtgt cgaagtccac catgatggcg tgcatctcga tttcgcctcc
      721 ggcgtaatcg cagaggttct ccgccacccg cccgcacagg aggtagggcg cgcgctccag
      781 gtgtgcggcg cgccacagct cgtaggcgtg gcgggcggtg ttggccccct tcacgtcctc
      841 gaggagttgt gcatccccac cggcctcctc tgtgatctcc gcgagaagct cgttgtccac
      901 cttcgagcgg gtccagtggg tcatcatgac gccagcggcc agcttggtca gcttccccgc
      961 catcccgacg aagaagccgc gcctgagccc gttcttgacc gcccctttta tggcctgtcc
     1021 tgtaaagtcc ccaacctcta tgaaacagac ctcctcgagg gcgggcagga gacgcatcgc
     1081 ggccctctcg gtgagccctc cggtcgagag aacgaaatgg tcgagaccct gcgcacccat
     1141 cacgtcgata gcctgcacca cgctcgcggc ccacgcggcg gtcgagaagg gacggacgat
     1201 gccggtcgtc ccgaggatgg agatgccgcc gacgatgccg agccgggcgt tcgttgtctt
     1261 ctccgccatc ttctcgccgc caggcacgct gatcacaacg cgtaccccgc gttctccagc
     1321 gtgcagcacc tcatcaagcg aataggagat catgcgccgc gggacgtcgt ttatagcggg
     1381 accgccgaca ggcagcccca gccccggctt cgtgactacg cccacgcctt ctccccggtc
     1441 cagcgagatc cccggctcct ccttccagga gacgcgggcc gtgaggcgcg ccccgtgcgt
     1501 cacgtccggg tcgtcgccgg catccttgat gacgacggcc tcggcccagc cctcccccac
     1561 ctcgcatcgc tcgacctcga aggagacgcg ccgcttatct cccttcccgg gaagcctcac
     1621 ctccacccta ccctgcggct caccggtaat gagagccgtc gcggcggctt tggctgcggc
     1681 tgcggcgcag gtccctgtgg tccagccggt cctgagcttc tcatccttgg cccgcgccat
     1741 gctcgggggc atggccggct cgcgcagctt ccggtcaggg ggcatctgcc ctctcctccg
     1801 cccggcggaa catgtgggag aactgcgggc tgtagaggtg agaacgggtg ccacccgccg
     1861 cgaggccagg gccgaccagg atcaaggcct gccgggtaaa tcccgcttcc ctgacgcggt
     1921 cggccagctc ctcgagtgga cactcgatga ccagctcgtc cggccagctc gctttgtaga
     1981 cgaccgcgca cggggtatcc ggcgcgtagc cgccctccag gagctcctcc tgcagggcgc
     2041 gcgggcgggc ggctgagagg aagatcgcca tggtcgtccc gtgagcggcg aagctcctta
     2101 tgtcctctcc atcgggcatc ggggtgcggc tggctctacg cgtcaggatc accgactgcg
     2161 agacctcagg caccgtcagc tcccgcccga tggccgcagc cgcagccccg agcgaggaga
     2221 cgccgggcac gatctcccac tccagcccga tctcctcgca taactccacc tgctccatca
     2281 cagcgccgta gatgctcggg tcgccggaat gtacgcgtgc caccctcaga tcttccgcca
     2341 cagccctctc gtagagctcg cgcacgcct
//
LOCUS       DASTUV010000485         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_342609, whole genome shotgun sequence.
ACCESSION   DASTUV010000485 DASTUV010000000
VERSION     DASTUV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342609"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFT03_09805"
     CDS             <1..225
                     /locus_tag="VFT03_09805"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEU4848484.1"
                     /translation="QVDELRPYGGEDYVIVSPYPGQDAPVIATSWRVQLKLDGAGDPR
                     LRKFVDQFRVSELAPLSGNRCAGGVGRPAA"
     gene            complement(507..1859)
                     /locus_tag="VFT03_09810"
     CDS             complement(507..1859)
                     /locus_tag="VFT03_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEU4848485.1"
                     /translation="METTKFLLDEKALPESWYNLVPDLPFALEPPLHPGTREPVGPEA
                     FAPIFPEEIIRQEVTGEQRVHIPEEVREIYSLWRPTPLFRARRLERLLDTPAHIYYKY
                     EGVSPTGSHKPNTAVPQAYYNREEGVRRLTTETGAGQWGSSLAFACGIMGLECTVYMV
                     RVSYDQKPYRRVMMQTYGAEVHASPTGLTQAGRNILEEDPDSPGSLGIAISEAVEDAV
                     KNDDAKYSLGSVLNHVLLHQTVIGQEALSQMELAGEYPDVVVGCVGGGSNFAGISFPF
                     IGENLTGGRETRIIAVEPASCPTLTKGRYTYDFGDTAGTTPLMKMYTLGHSFVPAGIH
                     AGGLRYHGDSPIVSALVHEGLVEARAYAQNATFEAGVAFARAEGIVPAPEVAHAIRAT
                     MDLALEARESGEEKVILFNLCGHGHFDLAAYEQYLAGNLVDLEHSEEEIEKAVAGIPD
                     "
     gene            complement(1944..3716)
                     /locus_tag="VFT03_09815"
     CDS             complement(1944..3716)
                     /locus_tag="VFT03_09815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU4848486.1"
                     /translation="MAYEFARYLKVRGAWGASWSPDGRRLAFLTEITGVPQVWEVEAG
                     GPSWPEQLTFYEERVSGAEYSPVDNRLLFGMDAGGNERTQLFLLERGEVTDLTRAPGA
                     IHYSGGFSPDGGRVAYTATRRNGTDFDVFVQDLDGVPEIVWEVSGYHTVSDWAPDGSA
                     LIVSRHHSNLNNDLYWLDPETGEATLLTPHEGDVRFSGARVAPDGRLYLATDRDGDFM
                     RLARLDPSTLDVTYLTPDDWDVEGVELSRDGRYLLASRNVEGYSDLMLFSGEGKRMPD
                     VAVPEGIVGGFEFSPDSRRLAFTLVGPTRNPDVWVLDLPDGEPRRVTRSSTAGIPRST
                     FRRPSLVRYPSFDGLEIPALLYEPGGAGGNAPVVVNVHGGPESQSRPGFAPVTQYLLQ
                     RGYAVFFPNVRGSTGYGKAYTHLDDVELRMDSVRDLAHAAEWLRERGHERVAVMGGSY
                     GGFMVLAALTEYPELWSAGVDIVGIANLVTFLENTGSYRRGLREPEYGSLEKDRAFLE
                     SISPIHKAGNITAPLMVIHGKNDPRVPVGEAEQIVERVRENGGTVEYLLYEDEGHGLA
                     KLKNRLDAYPKIADFLEEHLGTRG"
BASE COUNT          620 a         1323 c         1262 g          710 t
ORIGIN      
        1 caggtggacg agctgcgccc ctacggcggg gaggactacg tgatcgtcag cccgtacccc
       61 ggccaggacg cgcccgttat cgccacctcc tggagggtcc agttgaagtt ggacggcgcc
      121 ggagacccgc gtctccggaa gttcgtagac cagttcaggg tctccgagct tgcccccctc
      181 tctggcaacc gctgcgctgg cggcgttggc cgtccggccg cctgatacgg ccggtcaggc
      241 agacggtcgg agcacttcag gacaggtatg cctgcggcgc cacctgtgca acgtccatcg
      301 ttcctatgtg ccgtatacgt aggcgcagtc ctcacgtatt tttcggcacc ctcgaatgca
      361 caacacttat gggagaactt agtgggagga ccggcgtcgg gggtgtgccc cctcccaccc
      421 tcggcgctcc ggaagccagg ctacgctgtg cgcgccggcc cgacctcgaa ccctcctcag
      481 cgaggggaaa catccacaac agcgcttcag tccggtatac ctgcgacggc cttctctatc
      541 tcttcctcgg agtgctccag gtcaaccaga ttccctgcaa ggtactgctc gtaggccgcg
      601 aggtcgaagt ggccgtggcc gcagaggttg aagaggatga ccttctcctc gcctgactcc
      661 ctcgcctcga gcgccaggtc catcgtcgcc cttatggcat gtgcgacctc gggggcgggg
      721 acgatcccct cggccctggc gaaggctacc ccggcctcga aggtcgcgtt ctgggcgtag
      781 gcgcgcgcct cgacgagccc ctcgtgcacg agcgccgaga ctatgggcga gtcgccgtgg
      841 tacctgaggc ccccggcgtg gatgccggcc ggcacgaacg agtgccctag cgtgtacatc
      901 ttcatcagcg gcgtcgtccc tgcggtgtcc ccgaagtcgt aggtgtagcg ccccttcgtg
      961 agggtcgggc acgaggccgg ctcgacggcg atgatgcgcg tctccctgcc tccagtcagg
     1021 ttctccccga taaacgggaa ggagatcccg gcgaagttcg acccgccgcc gacgcagccg
     1081 acgaccacgt cgggatactc gccggccagc tccatctgag acagcgcttc ttgcccgatc
     1141 acggtctggt gaaggaggac gtggttgagc acgctcccta aagagtactt cgcatcgtcg
     1201 ttcttcaccg cgtcctcgac ggcctcggag atcgcgatgc caagagatcc cggcgagtcg
     1261 ggatcttcct cgagtatgtt gcgcccggcc tgcgtcaggc cggtcgggct cgcgtggacc
     1321 tcggcgccgt aggtctgcat catgacgcgc ctgtaaggtt tctggtcgta gctgacgcgc
     1381 accatgtaaa ccgtgcactc gaggcccatg atcccgcagg cgaaagcgag cgaagagccc
     1441 cactggcccg cgccggtctc ggtggtgagg cgcctgacgc cctcctcgcg gttgtagtag
     1501 gcctggggga ctgcggtgtt cggcttgtgg ctgccggttg gggagacgcc ctcgtacttg
     1561 tagtagatgt gggccggggt gtcgagaagc ctctccaggc gcctggcgcg gaacaggggt
     1621 gtcgggcgcc agagcgagta gatctctcgc acctcttcgg ggatgtggac gcgctgctcg
     1681 cctgtcacct cctgcctgat gatctcctcg gggaagatcg gcgcgaacgc ctcgggcccg
     1741 accggctccc tggtcccggg atggagcggt ggctcgagcg cgaacggcag gtccgggacg
     1801 aggttatacc agctctccgg cagcgccttc tcgtcgagca gaaacttcgt agtctccatg
     1861 tccaaaacct ccggctctca gcctttcagc tatcagctta cagaattcag aggccgcccg
     1921 cgccgcggtc gggttgcgct cgctcaacct ctggtgccca gatgctcttc caggaagtcg
     1981 gctatcttcg ggtaggcgtc gaggcggttc ttcagtttgg ccaggccgtg tccctcgtcc
     2041 tcgtagagca ggtactcgac ggtgccgccg ttctcgcgca ccctctcgac tatctgttcc
     2101 gcttctccta ccggaactct ggggtcgttc ttgccgtgga tgaccatgag cggagccgtt
     2161 atgttacctg ccttgtgtat ggggcttatg gactcgagga aggcgcggtc cttctccagt
     2221 gaaccgtact cgggctcgcg caagccgcgc cgatagctgc ctgtgttctc caggaaggtg
     2281 acgaggttgg cgatccccac gatatccacc ccggcgctcc agagctcggg atactccgtg
     2341 agcgcggcga gcaccatgaa cccgccgtat gagccgccca tgaccgccac gcgctcgtgg
     2401 cccctctcgc gcaaccactc ggccgcgtgc gccagatctc ttacggagtc catcctgagc
     2461 tcgacgtcgt cgaggtgggt atacgccttg ccgtagccgg tggagccgcg cacgttgggg
     2521 aagaagacgg cgtagccgcg ctgcaagagg tactgcgtca cgggagcgaa gcccggccgc
     2581 gactggctct ccggcccgcc gtgcacgttc acgacgacgg gagcgttccc gccggctccg
     2641 ccaggctcgt agaggagagc ggggatctcc agcccgtcga agctcgggta acgtaccagg
     2701 cttggccgcc ggaacgtgct cctcggtatc cctgcggtcg aggagcgggt gacccgccgc
     2761 ggctcgccgt cagggagatc caggacccac acgtccgggt tgcgcgtggg cccgacgagc
     2821 gtgaaagcga gcctcctcga gtcgggcgag aactcgaacc cgccgacgat gccctcgggt
     2881 accgccacgt ctggcatgcg cttcccctcg ccggagaaca gcatgaggtc cgagtacccc
     2941 tcgacgttgc ggctcgccag caggtacctg ccgtctctgg agagctctac tccttccacg
     3001 tcccagtcat caggggtcag gtacgtaacg tccagcgtcg aggggtcgag gcgcgcgagc
     3061 ctcatgaagt ccccgtccct gtcggtggcg aggtagagcc tcccgtccgg cgcgacgcgg
     3121 gcgccggaga accgaacgtc cccctcgtgc ggcgtgagca gcgtcgcttc accggtctcg
     3181 gggtccaacc agtacaggtc gttgttcagg ttggagtggt ggcgcgagac gatcagggcc
     3241 gagccatcgg gcgcccagtc ggagacggtg tggtagccgg agacctccca cacgatctcg
     3301 ggcaccccgt cgaggtcctg cacgaagacg tcgaagtccg tcccgttgcg cctcgtcgct
     3361 gtgtaggcta cccgccctcc gtcaggtgag aacccgcccg agtagtgtat ggcgccgggg
     3421 gcgcgggtca ggtccgtgac ctcgccgcgc tccagcaaga acagctgggt cctctcgttg
     3481 ccaccggcgt ccatcccgaa gagtagcctg ttatctaccg gcgagtactc cgcgcccgag
     3541 acgcgctcct cgtagaaggt cagctgctcc ggccacgagg ggccgccagc ttctacctcc
     3601 cagacctggg gcacgccggt gatctccgtc aaaaaagcca gccgacgccc gtcaggtgac
     3661 cagctcgccc cccacgcgcc cctgaccttg aggtatcggg cgaactcgta ggccaacgct
     3721 atcctctctc tcgactccag agacggaaga ttaaagcata gatccccggc caccctacat
     3781 caccacaccg ctatactcac gagaacgcgt tctcgtgagg aggtgccttc tttggtcttg
     3841 aggcggttgc gaagcatctt cggccaggag ccccggcgga ccggggctcc ctcgccgctc
     3901 ttcggcctgg aggca
//
LOCUS       DASTUV010000486         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_795483, whole genome shotgun sequence.
ACCESSION   DASTUV010000486 DASTUV010000000
VERSION     DASTUV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795483"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="mutL"
                     /locus_tag="VFT03_09820"
     CDS             complement(<1..850)
                     /gene="mutL"
                     /locus_tag="VFT03_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU4848487.1"
                     /translation="MKLFPQLVADEAVGYGGRVRVLAPEVAHRIAAGEVIERPASAVK
                     ELIENALDAGASRVEVDVEGGGVSLIRVRDDGSGMSPEDAARAVGRHATSKIVTADDL
                     ARVDTLGFRGEALHAIGAVSSLTLTTCERGNELGARVGVFAGEIVESALASHQPGTTV
                     EVRNLFLNLPVRRGFLGTQRAEANAVSAVVESLALSRPGVGFSLRSEGRGVLVLPAAA
                     DLRERISQVHGLALARTLVALDDPVVWGLVSPISVSFPTRRYTHVAVNGRVVEADSFG
                     PAVSRAY"
     gene            complement(855..>2355)
                     /gene="mutS"
                     /locus_tag="VFT03_09825"
     CDS             complement(855..>2355)
                     /gene="mutS"
                     /locus_tag="VFT03_09825"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU4848488.1"
                     /translation="LRRWLERPLLEADRINQRLEAVDALASDYMLREEAREHLSRIPD
                     IERIATRIVRLSASPNDLLSLKGALEALGPLKESLGSAARVSSLVHRALAAMEEPPGV
                     KELIAAAISEEEGEIIRAGYSRELDEAREFRDGAHDWLTRFETRERLKTGLKTLKVGY
                     RDGEGYFIEVGGKDTGNVPPRYEHRKALKHNARYVTVELKEHESRMLTARDEVERLER
                     RILSEIRAAVKDAAPELQKIARAVAVVDVISSFATAATELRYCRPEVTEERGLRVIAG
                     RHPVVEHNTETPFVPNDARVSGDSRLQIITGPNMAGKSVYLRQVALIALLAQTGSYVP
                     AEEATLGVVDRIFTRVGAEDRLASGESTFMVEMTEAASILNSATERSLVILDEVGRGT
                     STYDGMSLAWAIAEYLHDDVQALTLFATHYHELTRLAEMLPGCRNHKAIVEEIGGEIV
                     FLHRIEEGAESSSYGVHVARLAGLPRQVTDRAGEILSRLESEGVKDFAG"
BASE COUNT          365 a          803 c          797 g          390 t
ORIGIN      
        1 cgtaggccct ggagaccgcg ggcccgaagg agtccgcctc aacgaccctg ccgtttaccg
       61 ccacgtgcgt gtaacgccgc gtcgggaagc tcaccgaaat agggctgacc aacccccaca
      121 ctacggggtc gtcgagggct acgagggtgc gggcgagcgc gaggccgtgc acctgcgaga
      181 tgcgctcccg caggtcggcg gcggcaggca acacgaggac cccgcgcccc tccgagcgca
      241 gggagaaccc taccccggga cggctgagcg cgagcgactc gacgaccgcg gatacggcgt
      301 tggcctcggc tctctgggtg cctagaaacc ccctgcgcac cggcaggttc aggaacaggt
      361 tgcgcacctc gaccgtcgtc cccggctggt gggaggcgag tgcggactcg acgatctcac
      421 cggcgaagac gccgacgcgg gcacccagct cgttaccgcg ctcgcaggtc gtaagggtga
      481 gcgatgagac ggcgccgatg gcgtggaggg cttcgccgcg gaagcccaga gtgtcgacgc
      541 gggccaggtc gtcggcggtc actatcttgc tcgtagcgtg ccgcccgacc gccctcgccg
      601 cgtcctccgg tgacatgcct gagccgtcgt cgcgaacccg gatcagggag accccacctc
      661 cctctacgtc cacctctacg cgggatgccc cggcgtccag ggcgttctcg atcagctcct
      721 tgacagccga ggccgggcgc tcgatgacct cgccggcggc gatcctgtgc gccacctccg
      781 gcgcaaggac ccttaccctt cccccgtacc cgaccgcctc atcggcgacg agctggggga
      841 agagcttcac ggcgctaccc ggcgaagtcc ttgacgcctt cggattcgag gcgtgagagg
      901 atctcgccgg cccggtccgt gacctggcgc ggaagcccgg cgaggcgcgc gacgtggacg
      961 ccgtacgaag acgactcggc gccctcctcg atgcggtgga ggaagacgat ctcgccgcct
     1021 atctcctcta cgatagcttt gtggttgcgg cagccgggca gcatctccgc gagacgtgtc
     1081 agctcgtggt agtgggtggc gaagagggtg agggcctgca cgtcgtcgtg caagtactcc
     1141 gcgatcgccc acgcgaggct catcccgtcg taggtcgagg ttccccgtcc gacctcgtcc
     1201 agtatcacca ggctgcgctc ggtcgcgctg ttcaagatgc tcgctgcctc ggtcatctcg
     1261 accatgaagg tggactcccc gctcgcgagc cggtcctcgg cgcctaccct ggtgaagatc
     1321 ctgtcgacga cgccgagcgt cgcctcctca gcgggcacgt aggagccggt ctgggccagg
     1381 agcgctatga gggcgacctg gcgcaaatac acagacttgc ctgccatgtt gggaccggtg
     1441 atgatctgta gcctcgagtc cccgctcaca cgcgcgtcgt tgggcacgaa cggggtctcg
     1501 gtgttgtgct cgacgaccgg gtggcggccc gcgatgacgc ggagcccgcg ttcttcggtc
     1561 acctcggggc ggcagtagcg cagctctgtc gcggcggtag cgaaggagga gatcacatcc
     1621 accacggcaa cggcgcgcgc tatcttctga agctccggag ccgcgtcctt gacggccgcc
     1681 cttatctcgg agaggatcct gcgctcgagg cgctcgacct cgtcgcgcgc ggtgagcatc
     1741 ctcgactcgt gctccttgag ctcgacggtg acgtagcgag cgttatgctt gagcgccttg
     1801 cggtgctcgt agcgcggcgg gacgttgccg gtatccttgc ccccaacctc gatgaagtag
     1861 ccctcgccgt cgcggtagcc tactttcaag gtcttcaaac ccgtcttcaa gcgctcccgg
     1921 gtctcgaagc gggtgagcca gtcgtgggcg ccgtcgcgga actcgcgggc ctcgtccaat
     1981 tctcgggaat agccggccct tatgatctca ccctcctcct cggagatcgc cgccgcgatg
     2041 agctccttca ccccgggggg ctcctccatc gccgcgaggg cacggtggac cagcgagcta
     2101 acgcgcgcgg cggagccaag cgattctttg aggggtccga gggcttccag cgctcccttc
     2161 aacgagagca gatcgttggg agaggccgag agacgcacga tacgggtagc gatacgctcg
     2221 atgtcgggga tgcggctcag gtgctcgcgg gcctcctcgc gcagcatgta gtccgaggcg
     2281 agggcgtcca cggcttcgag gcgctggttt atgcggtccg cctcgagcag gggacgctcc
     2341 agccagcgcc tcaag
//
LOCUS       DASTUV010000487         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1265732, whole genome shotgun sequence.
ACCESSION   DASTUV010000487 DASTUV010000000
VERSION     DASTUV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265732"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFT03_09830"
     CDS             <1..197
                     /locus_tag="VFT03_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU4848489.1"
                     /translation="AYFPFGGGPRMCIGSNFARMEAVLLLATIAREFRLEPVTGGPPV
                     PQPSITLRPRGGIRMKLSKR"
     gene            236..706
                     /locus_tag="VFT03_09835"
     CDS             236..706
                     /locus_tag="VFT03_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEU4848490.1"
                     /translation="MTAQGSSLNRLAFSATAHCLTGCAIGEVLGLVIGTVLGWGNVAT
                     IVLAIVLAFFFGYSLTMLPLLRSGLSLATVLPLAFASDTLSITIMEIVDNLIVVLIPG
                     AMDAGLTSALFWGSLALALAVAFVAAFPVNRWLIARGKGHAVVHEYHHGGHEHH"
     gene            829..1182
                     /locus_tag="VFT03_09840"
     CDS             829..1182
                     /locus_tag="VFT03_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848491.1"
                     /translation="MHARGLNLKTLLIAAALAMLLLVGAGVAYATGSGDSGEQQATGP
                     GIEKAKSVALDHTNGGRVTGTEVGDEEGYYEVEVTRDDGSQVDVHLDRNFNVLSTPAD
                     HESSNDQDGPNDSGS"
     gene            complement(1375..2493)
                     /locus_tag="VFT03_09845"
     CDS             complement(1375..2493)
                     /locus_tag="VFT03_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4848492.1"
                     /translation="MVASSALLRERGFAEAKRLCYAGLDSPTLLREVAERLRRVVPFE
                     TYCAQSNDPLSGLLTYLGHDGVLGEKEVRLFLEHVYFEEELDAQRRLVQNRLPVALLS
                     EVTGGKLERAMRCREITAPIGLGYEVFSICSVGQAQWGGLDLMRERGDPDFDAREVTL
                     LHRIIPHLSSGLKAAVLAKQASAEPARESVPGVLVLDDRGRVVQHTEAAERWLRDLAD
                     LAEGWLEGEGLPPPVWMVVGALRKALKPETDRDLNGVPCVRVQTRSGRWLTFHGARTE
                     PRPGRGGETMVIIEPTRPQELAWLRVSAYGLSERERAVVDLVVQGASTREISQTLYIS
                     EYTVQEHLGNAFDKVGVRGRRALIKRLFFDNLYPGLLG"
     gene            2703..3110
                     /locus_tag="VFT03_09850"
     CDS             2703..3110
                     /locus_tag="VFT03_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4848493.1"
                     /translation="MPATAMNQTYAKLPARDIERARAFYADRLNLTPFGEHNSHLFYE
                     VGGSHFMVYPSSGAASGTHDQIGFTVEDVESMVAKLRSNGVNFEEYEPPSGASATDGI
                     MDFGGVKAAWFRDSEGNLISIVEFAGGSPFAAR"
BASE COUNT          632 a         1011 c          969 g          586 t
ORIGIN      
        1 acgcctactt cccgttcggg ggcggaccga ggatgtgcat cggcagcaac ttcgccagga
       61 tggaggccgt gctactgctc gcgacgattg cgcgcgagtt ccgcctcgag cccgtaaccg
      121 gaggaccacc ggtcccccag ccttcgatta cgctgcgccc cagagggggc atcaggatga
      181 agctgagcaa gagataaata tggaactaca acgaaattct acggcaagga gcgtgatgac
      241 tgctcaaggt tcttcattaa acaggctggc gttcagtgcg acggcgcact gtctgactgg
      301 ctgcgccatc ggcgaggttc tggggttagt gatcgggacc gtgttgggat ggggcaacgt
      361 agcgaccatc gttctggcca tcgtgctcgc gttcttcttc ggctactcgc tcaccatgct
      421 cccgctcttg cggtcggggt tgtcgctggc tacggtgctg cctctggcgt tcgcctcgga
      481 cacgctttcg atcacgatca tggagatagt ggacaacctg atcgttgtct tgattcccgg
      541 ggcgatggat gcggggctga ccagcgccct tttctggggc agcctggcgt tagcgctggc
      601 cgtcgccttc gtggcggcct tcccggtcaa ccgctggctc atcgcgcgtg gcaagggaca
      661 cgcggtcgtg cacgagtacc accacggggg ccacgagcac cactagacgg cgggagacct
      721 cgcggttccg ggatctcggt cgcgtaattc taatgccgtt ctcagctcat tctcaggttc
      781 agcgccctat actctgccca gaagctttag caacgaggag gtgatggaat gcacgccagg
      841 gggctgaacc tgaagacgct gctgatagcc gcggcgctcg ctatgttgct gctcgtcggg
      901 gcaggcgttg cttatgctac cggctccggc gactccggag agcagcaggc caccggaccc
      961 ggcatcgaga aggccaagag cgtcgcgctc gaccatacca acggaggccg tgtgaccggc
     1021 accgaggtcg gcgacgaaga gggctactac gaggtcgagg tcacccgcga cgacggcagc
     1081 caggtcgatg tccacctcga ccgcaacttc aacgtcctaa gcacgcctgc agatcacgaa
     1141 agctctaacg accaggacgg gccaaacgac agcggaagct gatcccacca ggagagatct
     1201 gcggaaggcc gggcttagat gcccggcctt ctctctttga ccctacaccc ttctctaaac
     1261 gatcccgcca agaagccagg agagagccca agcccccgaa tcaaagagct gacaagcgaa
     1321 gcccgtaggg cgtagcgtgc tgacaagccg accgaaggga ggaggcgtgc tgaatcaccc
     1381 gagcaacccg gggtagaggt tgtcgaagaa gagacgcttt atgagggcgc ggcgacctcg
     1441 tactcccacc ttgtcgaacg cgtttccaag atgctcctgg acggtgtact cggagatgta
     1501 gagtgtctgc gagatctccc tggtagaagc accctgcaca acgaggtcca cgaccgcccg
     1561 ttcgcgttcg ctcaacccgt atgcggagac tcgcagccac gccagttcct ggggtctagt
     1621 cggctcgatg atgaccatcg tctcgccacc gcggccgggt cgtggctcgg tccgggcgcc
     1681 gtgaaacgtg agccaccgcc ccgaacgcgt ctggacccgg acgcagggca cgccgttcag
     1741 atcccgatcc gtctcgggct ttagcgcctt gcgcagagcg ccgaccacca tccacaccgg
     1801 aggcggtagc ccctcgccct ccagccaacc ttctgcgagg tcagcgagat ccctgagcca
     1861 tctttcggct gcctcggtgt gttggacgac tcgtccccgg tcatcgagga ccagtactcc
     1921 tgggacgctt tctcttgcgg gctcagcaga agcctgcttg gccagtaccg ccgccttcag
     1981 gccgctactc aggtgcggga tgatccgatg tagtagcgtc acctcgcgag cgtcgaagtc
     2041 ggggtcgcct ctttcccgca tcaggtcgag accgccccac tgcgcctggc ccacgctaca
     2101 gatgctgaat acctcgtacc ctaacccgat cggagcagtt atctcccggc accgcatggc
     2161 gcgctcgagc ttcccacctg taacttcgga gagaagtgct accggcaagc ggttctgtac
     2221 cagcctgcgt tgtgcgtcca gctcttcctc gaagtagacg tgctcgagaa agagacgaac
     2281 ttccttctca cctagcaccc cgtcgtggcc caagtacgtc agaagcccac tgaggggatc
     2341 gttggattgg gcacagtacg tctcgaacgg taccacccgc ctcaggcgct cagccacctc
     2401 gcgcagcagt gttggcgagt ccaacccggc gtagcacagc cgcttggcct cggcaaagcc
     2461 tcgttcccta agaagagcac tactggctac catatcccca cgtcttcccg cgaaccccag
     2521 gcttactgag gtaacgctca gtagtcctgg ctcagcattt tcgcacagac gaccgtcttg
     2581 tcacaacgtt aacaaagcgg tggtaccacg aggtcgaaac cccaggtttc tggggtatga
     2641 ggccggctcg caaaaggcca tactcgtggc agcaaagcac aagggagaaa ggagaagaca
     2701 cgatgccggc tacagccatg aaccagacct acgcaaagct cccggcgcgc gacatcgagc
     2761 gagcgcgcgc cttctacgcg gacaggttga acctgacgcc gttcggcgag cacaacagcc
     2821 acctcttcta cgaagtcgga gggtctcact tcatggttta tccgagcagt ggagccgcgt
     2881 cgggcaccca tgaccagata ggcttcacgg tcgaggacgt cgagtcgatg gtggcgaagc
     2941 tgcgctcgaa cggcgtgaac ttcgaggaat acgagccgcc gtcgggagca tccgcgaccg
     3001 acggaatcat ggacttcgga ggcgtcaagg ccgcctggtt cagggacagc gagggcaacc
     3061 tgatcagcat cgtggagttc gccggcggat cgccattcgc ggcgcgttaa cggctcttcc
     3121 aagcagcttt gcctctaaag gcacgggggt tgagaagtcc cggtactttc tctgtatcta
     3181 gcgccgtatc agccagat
//
LOCUS       DASTUV010000488         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_875985, whole genome shotgun sequence.
ACCESSION   DASTUV010000488 DASTUV010000000
VERSION     DASTUV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875985"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFT03_09855"
     CDS             <1..1131
                     /locus_tag="VFT03_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU4848494.1"
                     /translation="EAEGATSILELLAAEDPAPDEVLDPATAVALLPYSSGTTGLPKG
                     VMLTHANLVTSVRQVRRGFPATGRDTLLAVVPFFHVMGFLLTLAAPLASGAAVVTMPR
                     FDLERFLALIQEYRVTVLVVAPPIMAALARHPMVDAYDLTSLELIVSGGAPLGADLQR
                     AVAERIPSAAVGQGWGMTETTVGATMPDRDFGTEPGSVGRVMPNTELRVVDPWTGRDL
                     AAGERGELWVRGPQVMAGYLNRPEATAGIIDAGGWLRTGDLGYVDGDGNVFVVDRLKE
                     LIKVSAYQVAPAELEAILVTHPQVADAAVIPRPDASHGEIPIAVVVRRGELDGDELMA
                     WVAGRVAPHKRIREVRFVEAVPRTPSGKILRRVLIEQERQMA"
     gene            complement(1160..1408)
                     /locus_tag="VFT03_09860"
     CDS             complement(1160..1408)
                     /locus_tag="VFT03_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848495.1"
                     /translation="MTVFRYGREHYEEGEYVRHKDAVPGDGRDPVVRIVEDLGTIEIP
                     CGKVDCRANCLEYLVHSSRKPEDYALAECKLSPLVSII"
     gene            1624..2403
                     /locus_tag="VFT03_09865"
     CDS             1624..2403
                     /locus_tag="VFT03_09865"
                     /inference="COORDINATES: protein motif:HMM:NF009499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4848496.1"
                     /translation="MVERLRGRVALVTGASRRRGIGAAICRALAEEVADVFFTYWTPF
                     DRTQPVGEDPEFPAALQEELRGMGVRAEGMELDLSLPDSPAKLLDAVTERLGLPSILV
                     NNAAYSTRDGFENLDAETLDAHYAVNLRATALLSVGFARRYPGGPGGRIINLTSGQSL
                     GPMLEELAYAATKGAIEAFTVTLAAEVGHKGITVNAVNPGPTDTGWMTEELERELEQK
                     FPLGGIGEPEDAARLVAFLAGDEARWITGQIIHSEGGFLRR"
     gene            2400..2975
                     /locus_tag="VFT03_09870"
     CDS             2400..2975
                     /locus_tag="VFT03_09870"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEU4848497.1"
                     /translation="MSARTLEAAWSQWDGPGLEHLRLRLGESGIEADGVVVGEEDGSC
                     FRARYVIICDPRWRTRELIFDPLDGSDPLHLRSDGEGDWRDASGSALLELRGCIAVDL
                     SVTPFTNTLPIRRLDLREGESSELAVVYVNVPVMELGASRQGYTCLERGPGGGLYRYE
                     DRGIFRGFTADLPVDENGLVLDYPGIFRRVS"
     gene            2985..3389
                     /locus_tag="VFT03_09875"
     CDS             2985..3389
                     /locus_tag="VFT03_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848498.1"
                     /translation="MEPAGPFWKLSQRSQKKLLEDVDPELDAPVKGPLFPRPVFLTLL
                     TVFGVAVMLAVDWFGLLLLNVEGVRLQMGSLNTLRVTAVLMAGIALPSVLLMIHFRRL
                     YSYVSDENVFFAIAFSLSFMLGFPCGVVGLFG"
BASE COUNT          682 a         1186 c         1332 g          710 t
ORIGIN      
        1 gaggctgagg gcgcgacatc catcctggag ttgctcgccg ccgaggatcc ggcccctgat
       61 gaggtgctgg atccggctac cgccgtagcg ctgctgccgt actcgagcgg cacgaccggg
      121 ctgccaaagg gggtcatgct cacgcacgcc aacctcgtca cgtcggtgcg ccaggtccgt
      181 cgcgggtttc ccgcgacggg acgcgacacg ctgctggcgg tcgtgccatt cttccacgtc
      241 atggggttct tgctcaccct cgccgcgccg ctggcgtcgg gggcggcggt tgtgacgatg
      301 ccgcgcttcg acctcgagcg gttcctcgcg ctcatccagg agtaccgcgt cacggtgctc
      361 gtggtcgcgc cgccgatcat ggcggctctc gcccggcacc cgatggtcga cgcgtacgat
      421 ctgacttccc tcgagttgat cgtctctggc ggagcgccgc tcggcgccga cctgcagcgt
      481 gccgtagcgg agcgtatccc cagcgcggcg gtcggtcagg gctggggcat gaccgagacg
      541 acggtagggg cgacgatgcc cgaccgcgac ttcgggaccg agcccggctc tgtcggacgg
      601 gtcatgccga acaccgagtt acgggtagtc gatccctgga ctgggcggga tctcgcggcc
      661 ggcgaacggg gcgagctgtg ggtcagggga ccgcaggtga tggccggcta cctgaaccgc
      721 ccggaggcta ccgctggcat catcgacgcc gggggttggt tgcgcaccgg cgacctcggt
      781 tacgtcgatg gggacggcaa cgtgttcgtt gtcgaccggc tcaaggagct catcaaggta
      841 agcgcttacc aggtggcacc cgcggagctg gaggcgatcc tggtcaccca cccgcaggtc
      901 gccgacgccg ctgtcatccc ccgcccggac gcgtcgcacg gtgagatccc gatcgccgtg
      961 gtagttcgtc gtggcgagct cgacggcgat gagctcatgg cctgggtggc cgggcgcgtc
     1021 gcgccacaca agcggatccg ggaggtccgg ttcgtcgagg ctgtcccacg caccccgtct
     1081 ggcaagatcc tgcgccgggt tctcatcgag caggagcggc agatggcgtg acccgcttca
     1141 ggccgggatg cgcctcactc tagatgatac tcacgagcgg agagagcttg cactccgcca
     1201 gagcgtagtc ctcgggcttg cgggacgagt ggaccaggta ttccaggcag ttggcccggc
     1261 agtcaacctt gccgcagggg atctctatcg tccccaggtc ctccacgatg cgcactacag
     1321 ggtcgcggcc gtcgcccggc accgcgtcct tatggcggac gtactccccc tcctcgtagt
     1381 gctcccgccc gtagcgaaat acggtcatag cgagattgta cactcgtttg acctggatca
     1441 catcgagggc agagagcgcg aagaggctga aatcttggtc ggctacaatc ccctgcggac
     1501 gaaaatgcca ggacccggaa ggacgatgag cgaaatatct acccttcagc ggaacgtcgc
     1561 aaggtgcgtg gaggaggtcg ggatcgaagc cccggtcctc gccgtcgaaa gatcggggcg
     1621 gtagtggtcg aacgtttgcg gggacgcgtg gccctggtta cgggggcgag ccggcgcagg
     1681 gggatagggg cggcgatctg ccgcgcgcta gccgaggagg tagcggatgt cttcttcact
     1741 tactggactc cattcgacag gacgcagcct gtgggggagg atcccgagtt cccggccgcg
     1801 ttacaggagg agttgcgcgg tatgggcgtc agggccgagg gcatggagct ggatctctcc
     1861 ctcccggatt cccccgcgaa gctactcgat gcggtgacgg agcggctggg attgccctcc
     1921 atcctcgtca acaacgctgc gtactccacg cgcgacggct tcgagaacct ggacgccgag
     1981 acgctcgacg cgcactacgc cgtgaacttg cgggcgacgg cgctgctcag cgtcgggttc
     2041 gcgcggcgct acccgggcgg gccgggcgga aggatcatca acctcacctc ggggcagtcg
     2101 ctcgggccga tgctcgaaga gctggcatac gctgcgacga agggcgccat cgaggctttc
     2161 accgtcacgc tcgcggcgga ggtcgggcac aagggcatca ccgtgaacgc tgtgaacccc
     2221 ggccccacgg acaccggctg gatgacagag gaactcgagc gggagctcga gcagaagttc
     2281 ccgcttggcg gtatcgggga gcccgaggat gcggcgcggc tcgtagcgtt cctggctggc
     2341 gacgaggcgc gctggatcac gggccagatc attcactccg agggcggctt cctgcgtcga
     2401 tgagcgccag gaccctcgaa gccgcgtggt cacagtggga cggaccggga ctcgagcacc
     2461 tgcgcctgcg cctcggagag agcggcatag aggccgacgg agtggtcgtc ggcgaggaag
     2521 acggctcctg cttcagggcg cggtacgtga tcatatgcga cccgcgctgg aggacacggg
     2581 agttgatctt tgacccgctc gacggaagcg acccgctgca cctccgttcc gacggagaag
     2641 gcgactggcg cgatgcttcg ggaagcgctc tcctggagct acggggttgc atcgccgtgg
     2701 acctctcggt aacgccattc actaacactc tgcccatccg gcgcctcgac ctgagagagg
     2761 gggagtcctc ggagctagcc gtcgtctacg tgaacgtccc ggtgatggag ttgggcgcat
     2821 cgaggcaagg gtacacgtgt ctcgagcgcg gccccggcgg cggcctctac aggtacgaag
     2881 acaggggtat attccggggg tttacggccg atctgccggt ggacgaaaac gggctcgtgc
     2941 tcgactaccc gggaatcttc cggcgggtgt cctagaatac gacgatggaa cccgccggcc
     3001 cgttctggaa gctctcgcag cgcagccaga agaagctgct tgaagacgtc gaccccgagc
     3061 tggacgcccc ggtcaaggga ccgctcttcc cgcgcccggt cttcctgacc ctgctgaccg
     3121 tcttcggcgt cgcggtcatg ctcgccgtgg actggttcgg gctcttgctc ctcaacgtag
     3181 agggcgtccg cttgcagatg ggatccctga acacgctgcg cgtcaccgcc gttctcatgg
     3241 ccgggattgc gctgccctcg gtcctgctca tgatccactt ccgccgcctc tactcctacg
     3301 tctccgatga gaacgttttc tttgctatag ccttctcgct ctcattcatg ttgggattcc
     3361 catgcggtgt tgtggggttg ttcggctgaa tccataggac tcaagtgttg gtcacctcac
     3421 ctgtttgaaa tagcgttaag cgttgtaaag ctcgattaca aaggggttgt acgcgcgaga
     3481 gaagaagagt acggttttgg tggccaaagc cggtgcacgt atctaggtga taggtgggac
     3541 aagggaaagg ggaatatggg caccggtaca gcccatagga caagtttgtg ttcgaatgta
     3601 ttaaatctat atgacaatcg gacataggtt gctgccgagg cacgtcgttg ctacgtacac
     3661 tcaagccatg actaacgagg attcggcgct agacgagatt caggacaaca ctagagaagc
     3721 ggggcaacgg ctcagagcat cgctcaacct catgcgttct caaggaatgc tggacgacag
     3781 caaatttcgt gagctagcta cgcggttgag cacggcgcta gctatgactg aagctgcata
     3841 cctcgaagcg agaaggcgta gggagatgta gctgtactgg tggaggctgg gttgcacctt
     3901 ggttcgctaa
//
LOCUS       DASTUV010000489         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_300663, whole genome shotgun sequence.
ACCESSION   DASTUV010000489 DASTUV010000000
VERSION     DASTUV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300663"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFT03_09880"
     CDS             complement(<1..1356)
                     /locus_tag="VFT03_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4848499.1"
                     /translation="MLTDISVENVALIEEATLELSPGLNAITGETGAGKTLLATALQL
                     LLGGRARSETVRAGARRATVEGTFVLPDETRYSILEEALGDLSEEVEDDDALVLRRTL
                     SGEGRSRCYVGGVTVPVRALASLGERMVSYHSQREQTRLTEPSEQLAILDDFLDEDAV
                     SARLEREALWHAVERDRRELEEITASSEARLREVDFLRYQVSELEAAGYSSQELSDLT
                     RERERLRNVTDLLQSTAAAAGAISPEEGGGAIDSVAGAVSELEKASRYDEALSQLADR
                     LGGLSAELGDVLYELRSYVDELEADPNRLEVVEDRLAALRALERKYGDVEGYLEEART
                     RLERLENVDEETAELEAKIMEGERRLGDLATRITAGRKRAAQELSAKVQENLADLNLE
                     KTTFTAELVPTSAGPSGVERVEFVIRPNPGEPERPVRRYASGGELSRIMLAIRLAQER
                     IE"
     gene            complement(1356..2165)
                     /locus_tag="VFT03_09885"
     CDS             complement(1356..2165)
                     /locus_tag="VFT03_09885"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU4848500.1"
                     /translation="MGERLDPQKIRRVAIVMKPGLSEGYFERLVAALEKRGVEIAEVR
                     HDDKPDGASEPDLVFVLGGDGTMLRAIRMYPGRVLLGVNLGKVGFMSGMLPEEIESGV
                     EKVFSGGLEVQEYRMLDVRVDGGEPRQAANEAVLFKKRPHQVISVDVRVAGEELFAFR
                     CDGFIAATPLGSTAYALSAGGPIVAGDAGCYVLVPIAPHALVGRPLALGVDQVTELEL
                     VGRDALLSLDGEEPREMPAGRRVEIKPSQESVKIGRTEDWSWWRAVRRTFL"
     gene            complement(2158..2949)
                     /locus_tag="VFT03_09890"
     CDS             complement(2158..2949)
                     /locus_tag="VFT03_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU4848501.1"
                     /translation="MKDAPQRLDALLVERGLAENRSRAQGLILAGSVRVGGQVVTKAG
                     LRVASGEPLTVETKDGFVSRAAEKLDGALEEFGVQVEERACVDVGASTGGFTDVLLRR
                     GAERVLAIDVGYGQLAWPLRNDERVVVLERTNVRYLTGDDLPFAPDLLVADLSFISLT
                     VALEKLVSTTPSLHEAVVLVKPQFEAGPELVGRGGLVRDPQVHAAVIRRVAGFFEELG
                     FGALGVARAPVAGRRAGNQEYPLHVVRGAEAVLEDARVREVVEGG"
BASE COUNT          492 a         1148 c          929 g          500 t
ORIGIN      
        1 ctcgatgcgc tcctgcgcga gcctgatggc gagcatgatc ctcgacagtt cgccgccaga
       61 ggcgtagcgc cttaccggac gttccggctc gccggggttg ggtcggatca cgaactccac
      121 cctctccacg ccggaaggac cggcagaagt gggtacgagc tcggcggtga aggtcgtctt
      181 ttccaggttg aggtcagcca ggttctcctg caccttcgcc gagagctctt gcgcagcccg
      241 cttcctgccc gccgttatcc tcgtcgcgag atccccgagc cgcctctccc cctccattat
      301 cttcgcctcg agctcagccg tctcctcgtc gacgttctcg aggcgctcga gtcgtgtcct
      361 ggcctcctca agataaccct ccacgtctcc gtacttgcgc tcgagagccc tcagggccgc
      421 gagacggtcc tccaccacct ccaaccggtt ggggtcggcc tcgagctcat ccacgtagga
      481 cctgagctcg tagagcacgt ctccgagctc cgccgagagc ccaccgagcc tatcggccag
      541 ctgcgagagg gcctcgtcgt agcgagaggc cttctccagc tcggagacag cgccggccac
      601 agagtcgatc gcgcccccgc cctcctcggg ggagatggca ccggccgcgg cagcggtgga
      661 ttgcaggagg tcggttacgt tgcgcaagcg ctcgcgctcg cgagtcaggt cagagagctc
      721 ctgcgagctg tagcccgcag cttcaagctc cgagacctgg tagcggagga agtctacctc
      781 ccgcagcctg gcctcggagg aggccgtgat ctcctcgagc tcccgcctgt ctctctcgac
      841 ggcgtgccag agcgcctcgc gctcgagcct tgctgagacg gcgtcctcgt cgaggaaatc
      901 atctaggatg gcgagctgct ccgacggctc ggtgaggcgc gtctgctccc gctggctgtg
      961 gtaggagacc atccgctcgc ccagagaagc gagcgccctg accgggaccg taactccgcc
     1021 cacgtagcag cgcgagcgcc cttccccact cagcgtccgc cgcaggacca gagcgtcatc
     1081 gtcctccacc tcctcggaga gatcgcccag ggcctcctcg agaatcgagt agcgcgtctc
     1141 gtcggggagg acgaacgtgc cttcgacggt ggctcgcctg gcgcccgcgc gcaccgtctc
     1201 ggagcgagca cggccgccga ggagcagctg caaggccgtg gcgagcaggg tcttccccgc
     1261 cccggtctca ccggtgatcg cgttcagccc aggagacagc tccaaggttg cctcctcgat
     1321 cagggccacg ttctcaacgg agatatccgt taacactaca ggaacgtcct tcgcacagct
     1381 ctccaccagc tccagtcttc ggtccggcct attttaacgc tttcttggga cggcttgatc
     1441 tccacccgcc ggccggccgg catctcgcgc ggctcctcgc cgtccaggga gagcagcgca
     1501 tcccggccca cgagctccag ctcggttacc tgatccaccc ccagagccag cggccggccg
     1561 acgagcgcgt gtggcgctat ggggacgagc acgtagcagc ctgcgtcgcc ggcgacgatc
     1621 ggaccacccg cagagagcgc gtaggccgta gacccgagag gcgtcgccgc gatgaaaccg
     1681 tcgcacctga aggcgaagag ctcttccccc gccacccgca catccaccga gatgacctgg
     1741 tgcggccgct tcttgaagag cacggcctcg tttgcggcct ggcgcggctc cccgccgtcg
     1801 actcgcacgt cgagcatccg gtactcctgg acctcgagcc cccctgagaa gaccttctcg
     1861 acccctgact cgatctcctc agggagcatc cccgacatga agccgacctt cccaaggttc
     1921 accccgagga gcacccgccc gggatacatc ctgatggccc ttagcatcgt accgtctccg
     1981 ccgaggacga agacgaggtc cggctccgag gcgccgtccg gcttgtcgtc gtgcctgacc
     2041 tcagcgatct ccacccccct cttctccagc gccgcgacga gccgctcgaa gtaaccttcg
     2101 ctgagccccg gcttcatcac gattgcgacg cggcgtatct tctgcgggtc caggcgctca
     2161 cccaccctcg accacctccc ggacgcgcgc gtcctccagg actgcttccg ctcctcgcac
     2221 cacgtgcagc gggtactcct ggttccctgc ccggcgcccg gctaccgggg cgcgcgccac
     2281 gccgagggcc ccgaacccca gctcttcgaa gaacccggcg acgcgccgga tcacagccgc
     2341 atgaacctgc ggatcgcgca ccagtccgcc acgcccaacc agctcgggac ccgcctcgaa
     2401 ctggggcttg accagcacga cagcctcatg cagtgatggt gtggtggaga ccagcttctc
     2461 cagcgcgacg gtgagggaga tgaaagatag gtcggcgacc aggagatcgg gtgcgaaggg
     2521 aaggtcgtcg cctgtgaggt agcgaacgtt ggtccgctcc agcacgacca cccgctcgtc
     2581 gttccggagc ggccaggcta gctgcccgta gcccacgtct atggcgagga cccgctccgc
     2641 gccccgccgc aggagcacat cggtgaaccc gcccgtcgag gcgccgacat cgacacatgc
     2701 ccgctcttcc acctgcaccc cgaactcttc cagagcgccg tcgagcttct cggcggcgcg
     2761 agagacgaag ccgtctttcg tctcgacggt gagcggctcg ccagaggcga cgcggaggcc
     2821 tgccttcgtc actacctggc cgccgactcg cacggagccg gcgaggatca aaccctgcgc
     2881 cctactcctg ttctcggcga ggcccctctc gacgagcagg gcgtccaacc gctgcggcgc
     2941 gtctttcacc tgtgagtcag ctctctcggt ggcgcacgaa gaaggcgagg tcccgcaaac
     3001 ccgaggtgtc gccgtccagc ctaccgagag ccgcgagggc cccctcgagc accctgtctg
     3061 cctcgctcc
//
LOCUS       DASTUV010000490         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_587851, whole genome shotgun sequence.
ACCESSION   DASTUV010000490 DASTUV010000000
VERSION     DASTUV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587851"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1279
                     /locus_tag="VFT03_09895"
     CDS             119..1279
                     /locus_tag="VFT03_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU4848502.1"
                     /translation="MTPVARESLAPALAPFGKSSTLPSEAYTSESVFRWELDHFFDRT
                     WVCVGRAEDLRSAGDQRAVAVGLESALLTRDEEGGLHAFYNVCRHRGHELLEAGCGTN
                     QKFIRCPYHAWAYGLDGALAGAPGFGKLAGFDRDGYSLVPLRIVEWHGWLFANASGDA
                     PAFGDHIGNLGELIKNHRPEELVARARHDYEVAANWKVITENYHECYHCSVIHPEFSR
                     VSPPTSSLNLEPEGLWVGGPMDLAEGTQTMSLSGESQAPTLPHLAEGQERKVYYFGLF
                     PNLLLSVHPDYIMTHRLRPLAPDRTAIECEWLFSREAVEAEGFDPSYASYVWDITNRQ
                     DWRACEGVQRGVSSRGYRQGPLSPREDAVYQFITMVARGYLEGKAAPPASRT"
     gene            1349..2566
                     /locus_tag="VFT03_09900"
     CDS             1349..2566
                     /locus_tag="VFT03_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU4848503.1"
                     /translation="MSQPPRVDLFDPSFKADPYPTYAKLRSSAPVYRAALPDGRGVWL
                     ITRYDDVLAVLKDQRFVKDWRGALTPEQLAQVPPIPEVMKPLSQNMLDTDPPDHERLR
                     ALVSKAFTPRLIERLRPRVQAISDGLLDAVQDRGEMDLIDDYAFPLPITVIAELLGVP
                     AEDRNNFREWSDAAVSGNASQEYMEQILIPHMQAFTDYLRALFEEKRSNPKDDLVSAL
                     VRAEEAGDRLSEDELLGMVFLLLVAGHETTVNLIGNGVLALLQHPDQLRKLKEDPSLI
                     KPAVEELLRYDGPVETSTERFAREDVEIGGQVIPRGEMVLVVLAAADHDPERFPDPDE
                     LDITRTDNRHLAFGKGIHHCLGAPLARMEGQIAISTLLRRMPNLRLKGSPESLSWRPG
                     MILRGLRGLPVEF"
BASE COUNT          686 a         1224 c         1090 g          627 t
ORIGIN      
        1 ccgacaacga taacgtcatg attgcccgca tccgacacca tgccaccccc tttgacgccg
       61 gatcgaaagt ctagtaaata tgacccatta cagcaaacgc caagggggtg ggtccgccat
      121 gacgcctgtt gctcgggaat ctctcgctcc tgctctagcc ccattcggca agagcagcac
      181 cctcccctcc gaagcttaca cgtcggagtc ggtgttccgg tgggagctgg atcacttttt
      241 cgacaggacc tgggtctgtg tggggcgcgc ggaggacctg cggagcgcgg gcgaccagcg
      301 cgctgtcgcg gtcggcctcg agtcggccct gctcacccgc gacgaagaag gagggctgca
      361 cgccttctac aacgtgtgcc gccaccgcgg tcacgagctc ctcgaggcgg gctgcgggac
      421 caaccagaag ttcatccgct gcccttacca cgcgtgggcg tacgggctcg acggggctct
      481 cgcgggcgct ccggggttcg ggaagctcgc gggcttcgac agagacggtt actcgctcgt
      541 gccgctccgc atcgtcgagt ggcacggctg gctcttcgcg aacgccagcg gtgacgctcc
      601 cgcgttcggg gaccacatcg gcaacctcgg ggagctgata aagaaccacc gtcccgagga
      661 gctcgtggcg cgcgcccgcc acgactacga ggtagcggcg aactggaagg tcatcaccga
      721 gaactaccac gagtgctacc actgctccgt catccacccc gagttctcgc gggtgagccc
      781 ccctacgagc agcctcaacc tcgagccgga agggctctgg gtcggtggac ccatggacct
      841 ggccgagggc acgcagacca tgtcgctttc gggcgagagc caggccccca cgttgccgca
      901 cctggccgaa ggccaggaga ggaaggtcta ctacttcggg ctgttcccga acctgctcct
      961 gagcgtccac cccgactaca taatgaccca ccgcctaagg cccctcgccc ccgaccggac
     1021 ggccatcgag tgcgagtggc tcttctcacg cgaggcggtc gaggctgaag gcttcgaccc
     1081 ctcctacgcc agctacgtct gggacataac caacaggcag gactggcggg cgtgcgaagg
     1141 cgtccagcgg ggcgtctcct cgcgcggtta ccgtcaggga cccctctcac ccagggagga
     1201 cgccgtctac cagttcatca ccatggtcgc cagaggctac ctcgaaggca aagctgcgcc
     1261 gccggctagc cgaacctgac gggctgagtc ccgccggcgt ctccgcacgt atagtaggaa
     1321 aacaaccagc caacgagagg aaagaagcat gtcgcagcct ccacgggtcg acctctttga
     1381 cccctccttc aaagcggacc cctaccccac ctacgccaag ctgcgctcga gcgcccctgt
     1441 ctaccgcgcc gctctgccgg acggtcgtgg agtgtggttg atcacgcgct acgacgacgt
     1501 cctggcggtc ctcaaagacc agcgcttcgt caaggactgg cgcggcgccc ttactcccga
     1561 acagctcgcc caggtcccgc cgatacccga agtgatgaag cccctcagcc agaacatgct
     1621 cgacacggac ccgccagacc acgagcgcct gcgagcgctg gtctcaaagg ctttcacgcc
     1681 gcgcctcata gagcggctgc gcccacgcgt ccaggcgatc tccgacgggt tgctcgacgc
     1741 ggtacaggac aggggcgaga tggacctcat agacgactac gcgttcccgc tgcccatcac
     1801 cgtcatcgcc gagttgctcg gcgtgcccgc agaagacaga aacaacttcc gcgagtggtc
     1861 ggacgccgcc gtctcaggca acgcttccca ggagtacatg gagcagatac tcatcccgca
     1921 catgcaggcc ttcacggact atctgcgcgc tctcttcgag gagaagcgct cgaaccccaa
     1981 agacgacctc gtcagcgcgc tggttcgagc ggaggaggcc ggcgacaggc tctcggaaga
     2041 cgagctgctc ggcatggtct tcctgctcct ggtagcgggc cacgagacca ccgtcaacct
     2101 cattggcaac ggcgtgctcg cgctgctaca acacccagat cagctccgga agctcaagga
     2161 agatccttcg ctcatcaagc ccgctgtcga ggagttgctg cgctacgacg gacccgtcga
     2221 gacctcgacc gagcgattcg ccagggagga cgtagagata ggcggccagg tcatcccaag
     2281 aggcgagatg gtgctggtag tgctcgcggc cgccgaccac gacccggagc gcttcccgga
     2341 cccggacgag ctggacatca cccgcaccga caacaggcac ctcgccttcg gcaaaggcat
     2401 ccaccattgc ctgggcgcgc cgctcgcccg gatggagggc cagatcgcca tctccaccct
     2461 gctgcgccgg atgcccaacc tgcggctcaa gggatcgccc gaatccctga gctggcgccc
     2521 cggcatgata ctgcgcggcc tgagagggtt gcccgtagag ttctaatgtc acacatgatt
     2581 agggctctcc ctgaacattt cgctatagag ggctggccag cggcagcatc tcgcggggta
     2641 aggcgctcaa gctgatggga gcagccctcg taggaggcac gctggcatcc ctcggaatgg
     2701 tggaggctgc tgccgatcct cctggctgta agcagggaag gcgtgtaaga aggccaagca
     2761 gtgctgtagc ggaaactgct ccagcagcgg cacctgcgct gcttgtccgt ctggtaaagt
     2821 cgagctcagc aacggcacat gcgcgacatc ctgcgagtcg aatgtaagag tgtacttgtg
     2881 gtagtgctgg ctgtaatgta gccattggcg gtgttgtagc ctactgcatt gatatcccta
     2941 gcagcaccca gtctgagtgc ggaacccaca acgaatgtcc tcgcggggag ttttgtaatg
     3001 ctcgcggctt ctgtacacgt gcatgctagg tgactaaggg aaggacccgg gttagtcttt
     3061 aggacgagcc ccggccctta tcgcgcacag accccggtcc cggcctaaat agtgctaacc
     3121 ctagcccatc tgggctaggt aaagatcgct cattttgggg atgcgcggtc gtcacgcgcc
     3181 gcataggatg ggcgcagttt cagaaccgga ccctgggcaa tcccatcctt cctccttcta
     3241 gcctatccgg ttctacgcct tgcccaggcc ggggtcctac ggggttccgg cctcttgcta
     3301 ccatcctcat tcgggccggg acgtggcaac gtaacctccc ctccccttcg cttaatcccg
     3361 gtccgatgcc ccatacatcc cctagcatcg gggctaatgt gcttgcacga gccaccataa
     3421 aaccccgcga tacagactag ctatgcggcg gtcctagccc gtcggactgg ggctagcgtc
     3481 atagcgcgat gtagatacgg cgacccccta cccgtctgag atcacggtgt cgggccttgg
     3541 ctcctcgata tcggacgtga acgtcaccgt aagcgggctg agccacacct tccccgacga
     3601 tgtcgggctg ctgctcgtct cgcccgc
//
LOCUS       DASTUV010000491         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1087615, whole genome shotgun sequence.
ACCESSION   DASTUV010000491 DASTUV010000000
VERSION     DASTUV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087615"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFT03_09905"
     CDS             <1..1112
                     /locus_tag="VFT03_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4848504.1"
                     /translation="EEFPELMEEWDRAWSGRPEPGYEKELPSWNPSETQKLATRKAGA
                     AAMNAFKDYVPTMIGGAADLVNSTSTAFSGGGMFSSTYVGRNIAWGVREHAMGSAVNG
                     ICVHGGMVRPYGSTFLIFSDYMRPAVRLSALMGIPVAWVYTHDSVALGEDGPTHQPVE
                     HYMALRAIPGLTVIRPADANEAAMAWRSTLEAKGPVAMLLTRQNVPVLDPKATRGALR
                     GAYVLAEAEGGEPDAILIGTGSEVSVALEARNLLAGQGVSARVVSMPSWEIFEKQDEV
                     YKNFVLPQNIGARISVEAGVTLGWERYVGFKGASIGIDRFGASAPGETVLEKLGVTPE
                     KVANETLELLGRSERVEEGSGTPAVEATSSEEGHS"
     gene            1183..2280
                     /gene="tal"
                     /locus_tag="VFT03_09910"
     CDS             1183..2280
                     /gene="tal"
                     /locus_tag="VFT03_09910"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU4848505.1"
                     /translation="MNNERLRKLQEIGQSVWIDSLSRDEIDNGSLERMIEDGVVGVTS
                     NPTIFQKAISASSLYDEQLQELSQKLDDPKEIFWELASTDIRNACDLLMPVFERTDRL
                     DGYVSLEVSPDVAYDTPRTVEEAMHLHELVDRPNLLVKIPATLPGLAAIEEMTAMGKS
                     INVTLIFSLSRYRAVAGAYFRGLQRLVADGGDPSGVASVASFFVSRVDTEADKRLDEV
                     GREDLKGRLAIENAKLAYEEFERIFSGGDWEFLEAKGATKQRPLWASTSTKNPDRRDV
                     IYVENLIGPNTVNTMPASTLEATMDHAEIRPTLEGDADDARRFLEDLREAGVSYEEVV
                     EVLEKEGVQKFAQSFEELREQIKSKGRQLVR"
     gene            complement(2353..2826)
                     /locus_tag="VFT03_09915"
     CDS             complement(2353..2826)
                     /locus_tag="VFT03_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848506.1"
                     /translation="MGLKARVLAGALGGVVATLVLSGLREALTRIGLVFETAPMQVVE
                     RMEEVGLLEEWSPGARRVLTVAAHFAYGTGTGTAFGFLRRERGGSREEAAVGAALGLL
                     AWGVGWASWLPLAGVHRAPWTQRTPKVLLPVLDHAVFGAAWGLAYWALGGRARRD"
     gene            3103..3314
                     /locus_tag="VFT03_09920"
                     /pseudo
     CDS             3103..3314
                     /locus_tag="VFT03_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558144.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          638 a         1049 c         1068 g          559 t
ORIGIN      
        1 gcgaagagtt ccccgagttg atggaggagt gggatcgcgc atggagtggt cgtcccgagc
       61 ccggctacga gaaagagctg ccctcctgga acccctccga gacccagaag ctcgccacgc
      121 gcaaggccgg ggccgccgcc atgaacgcct tcaaggatta cgtcccgacc atgatcggcg
      181 gagcggcgga cctcgtcaac tccaccagca ccgccttttc gggcggcgga atgttctcct
      241 cgacctacgt cgggcgcaac atcgcttggg gggtcaggga gcacgcgatg gggagcgccg
      301 taaacggcat atgcgtgcat ggcgggatgg tcaggcctta cggatcgacg ttcctgatct
      361 tctctgacta catgcgccca gccgtgaggc tctcggccct gatgggcatc cctgtagcct
      421 gggtctacac ccacgactcg gtagccctgg gcgaggacgg gccgacgcac cagcccgtcg
      481 agcactacat ggccctgcgc gccattcccg gcttaactgt catccgtccc gccgacgcca
      541 acgaggccgc gatggcctgg cgctccacgc tagaggccaa gggtccggtc gccatgctcc
      601 tgacgcgcca gaacgtcccc gtcctcgacc ccaaggccac aaggggcgct ctgcgcgggg
      661 cctatgtgct ggcggaagcc gaaggcggcg agcccgacgc gatactcatt ggcaccggct
      721 ctgaggtctc tgttgccctg gaggctcgga acttgctggc cggccagggc gtgtcggcgc
      781 gggtcgtgag catgccctcg tgggagatct tcgagaagca ggatgaggta tacaagaact
      841 tcgtactgcc gcaaaacatc ggggcccgga tctccgtcga ggctggggtt accctgggct
      901 gggagcgcta cgtcggtttc aagggagcct ccatcggcat cgaccgcttc ggcgcttccg
      961 ctcccggcga aactgtgctg gagaagctcg gcgtaacccc tgagaaggta gctaacgaaa
     1021 cgctcgagct cctgggtagg tccgagaggg tggaggaggg gtccggtacc cctgcggtcg
     1081 aggctacgtc ctcagaggag ggacattctt agatcaatag cgcgttgtag attgttgtga
     1141 cgaactgcaa agggcccagt aggagaccct aggaggttac gcgtgaacaa cgagcgtcta
     1201 cggaagttgc aagagatcgg gcagagtgtc tggatagact ctctctcccg cgacgagatc
     1261 gacaacggaa gcctggaacg catgatcgag gacggagtcg taggcgtcac ctcgaacccc
     1321 accatcttcc agaaggcgat ctccgcatcc agtctctacg acgagcaact ccaggagcta
     1381 tcccagaagc ttgacgaccc gaaggagatc ttctgggagc tggccagcac agacatccgg
     1441 aacgcttgcg accttctcat gccggtcttc gagagaactg atcgcctcga cggctacgtc
     1501 tctttggagg tctcacccga cgtcgcctat gacaccccga ggaccgtcga agaagccatg
     1561 cacctccacg agttggtgga taggccgaac ctcctcgtga agatacctgc gacgctgccg
     1621 gggctggcgg ccattgagga gatgaccgct atgggcaagt ccataaacgt caccttgatc
     1681 ttctcgctct cccgctaccg cgcggtcgcc ggggcgtatt tccggggcct ccagcgcctc
     1741 gtcgctgatg gcggcgatcc ctcaggcgta gcgagcgtcg cgagcttctt cgtctcccgg
     1801 gtggacaccg aggcggacaa gagactcgac gaggtcgggc gcgaggacct caagggccgg
     1861 ctcgccatcg agaacgccaa acttgcctat gaagaattcg agaggatctt ctccggaggc
     1921 gactgggagt tccttgaggc aaagggtgcc actaagcagc gccccctttg ggcttcgacc
     1981 tcaacgaaga acccggaccg cagggacgtg atctacgtag agaatctcat tgggccgaac
     2041 acggtgaaca ccatgcccgc ctccacactt gaggcgacga tggatcacgc agagattcgc
     2101 ccgacccttg aaggggatgc cgacgatgcc cgccgcttcc tcgaggacct tcgggaggcc
     2161 ggggtgagct acgaggaggt agtggaggtg ctggagaagg agggcgtcca gaagttcgcg
     2221 caatcctttg aggagcttag ggaacagata aagagtaagg gtcgtcagtt agtgcgctag
     2281 tcccgagcga gtgttctcag aagtccgggc ggactcggtg tcccggcggt cgcactcatg
     2341 cgcgaccctt gtctagtcgc gcctcgcgcg cccgccgagt gcccagtacg ccagccccca
     2401 cgcggccccg aacaccgcgt gatccaacac ggggagtagg accttgggcg tgcgctgcgt
     2461 ccagggcgct cggtggacac cggcgagcgg cagccagctg gcccacccca cgccccaggc
     2521 gagtagcccc agcgccgcgc ccacggcggc ctcctcccta gacccgcccc gttcccgacg
     2581 caagaagccg aaagccgttc cggttcccgt gccgtaggcg aagtgcgccg ccacggtcag
     2641 gacgcgccgt gctccaggcg accattcttc caagagcccg acttcctcca tgcgctccac
     2701 cacctgcatc ggggctgtct cgaagaccag cccgatcctt gtcaaggcct cgcgcaagcc
     2761 cgataagacg agtgtcgcca cgacaccgcc gagcgctccc gccagcaccc tcgctttgag
     2821 acccatagta ccctcctcgt gccgctccca gtcccattgt acccggccga ggtctggcct
     2881 agagctgggc cctggttccc agtcattccg ttactatgtc gggtggaggg gcaaaggaga
     2941 gctaggggag gaggtctggg cacattcaac gcagaggcct acctcgccgt ggaggaaaac
     3001 agacggcgca tggagtgggg cgccgaggtc gagcgcgact actcgggctg gctagacccc
     3061 agaagcgcat ctttagaagg agggtgacga gatgctgagc ctaggctcct ggtaggtagc
     3121 gcccactaca tcctgggtcc gttcggctcg gattctgacc tgaggcgtca cggagagaag
     3181 atagcgtccc gcgggggcaa gaacgcaaag aagcgagcgg cggtggccgt ggcgaggaag
     3241 ctctctcggt gctcttgcac ggtttgtggg ctagcggaga gctctacgat ccgctctaca
     3301 acacgcatcg gtgc
//
LOCUS       DASTUV010000492         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_229257, whole genome shotgun sequence.
ACCESSION   DASTUV010000492 DASTUV010000000
VERSION     DASTUV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229257"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFT03_09925"
     CDS             <1..578
                     /locus_tag="VFT03_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848507.1"
                     /translation="RFGVEVARDVVRPNVTGLVEAGAEWVQIDEPAATTRPEEVPLVV
                     ETFNATREGVDVRASMHICFSDYNSLFPHIEALDDCYELQLEFSNRDARDLGTKAEDR
                     PGYDVLERFARSAWDGKIGLGVIDIHTNFIEPPELVRDRILYAARVLGPERIEVNTDC
                     GLRTRTWEVSLGKLKNMVEGTRLAEQVLNGS"
     gene            658..945
                     /locus_tag="VFT03_09930"
     CDS             658..945
                     /locus_tag="VFT03_09930"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEU4848508.1"
                     /translation="MPALEDAGYILRLPEIERYLLLRDRLREDAEDRKLYARTKRDLA
                     SRSWPSMDHYAEAKTEVVEGIIARAADVGGGRERASAHCHSGTFENSISGS"
     gene            complement(1152..2072)
                     /locus_tag="VFT03_09935"
     CDS             complement(1152..2072)
                     /locus_tag="VFT03_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU4848509.1"
                     /translation="MAFRSVGINLPRLFLEGVSGLADDLEALKIAGPDFAEVWPAHLG
                     VILGGSLDAERSQRVRERLLKTDLAYTVHAPLEVNLMDLTAHDVQRDVLKASIWFANS
                     IGADVVVCHAGRRLGSRDARYSLQEQLAAERDALREAGDLAGELGVTIAVENYYPELL
                     IISGVVYDYSVWPSELAEQISAVDHPAVGLCLDVGHAALAAGVFGFDYFEECAAVAPL
                     VRHIHLHDNLMRTNVTGEPPVSEHPFYGLGDLHLPPGRGTIPLDEMFRRVNFPENPAC
                     CVELSPDLYSLAPEAIRAARELIEPVVVAR"
     gene            2244..3077
                     /locus_tag="VFT03_09940"
     CDS             2244..3077
                     /locus_tag="VFT03_09940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848510.1"
                     /translation="MSSYENYTQKAGNYDKTREPVGTEIIVGCFAHAPVPLDRMRVLD
                     AGSGTGSYSQALLAYVGRIEAVDLNPGMLEVAARKLAGSQAEGRINFYAARIDELPFE
                     DSALDGIMINQVLHHLPDEASKGFPAHRRVFREFARVLKPGGVLTVNTCSQEQLRHGY
                     WHYSLIPEAADALRNRYAPLDELIGILDDYGFSRRGRFVPIDATVQGESYFDPRGPLD
                     KEWRDGDSIWSLVTEDRLDQALSLVQKLDERGELEAYVARNDAWRPHIGQATILFASR
                     R"
     gene            3186..4163
                     /locus_tag="VFT03_09945"
     CDS             3186..4163
                     /locus_tag="VFT03_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEU4848511.1"
                     /translation="MDEVYSALTRIPLFKGVRPEAVNLEPLTSLTNATYKVTTGGLAY
                     TLRLPGKDTYEYVDRAAEEHNARNMAAAGVNTKVLYFDATDGTMLSEFVEGDTMNEER
                     FKRDSESPARVALALKWVHHLGPVFKSHFDVFSMIDVYLGILRKLQASLPEEYHEVEQ
                     GAEAVRRVLEASPMPLSPCHNDPWPGNLLDAGGRLYIIDWEFSGMNDPLWDLGDLSVE
                     AGFGPKQDRVMMEAYYGGAAPQALYARLALYKAMSDLLWALWGLIQHANDNPRNDFLT
                     YALGRLRRCKTLMSSTDFGRHLDAVRVRRKDLPTLSQSSLGYLNDSILP"
     gene            4249..4371
                     /locus_tag="VFT03_09950"
     tRNA            4249..4371
                     /locus_tag="VFT03_09950"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4332..4334,aa:Ala,seq:tgc)
BASE COUNT          924 a         1326 c         1358 g          836 t
ORIGIN      
        1 ggcggtttgg ggtggaggtg gcgcgtgacg tggtgcgtcc gaacgtcaca gggctcgtcg
       61 aggcgggcgc ggagtgggtc cagatcgacg aacccgcagc aacgactcgc ccggaggagg
      121 tgccactcgt cgtcgagacc ttcaacgcaa cgcgcgaggg cgtagacgtt agagccagca
      181 tgcacatctg cttctccgac tacaacagcc tcttccccca catcgaggcg ttggacgatt
      241 gctacgagct acagcttgag ttctccaacc gtgacgccag ggatctcggc acgaaggccg
      301 aggaccgtcc cggctacgat gtgctcgaga ggttcgccag gagcgcgtgg gacggcaaga
      361 tcgggctcgg cgtcatagac atccacacga acttcatcga gccacccgag ctcgtgcgcg
      421 accgcatact ctatgccgcg cgcgtgctcg gcccggagcg catcgaggtc aacaccgact
      481 gcggcctccg tactcgcaca tgggaggtct ccctggggaa gctaaagaac atggtcgaag
      541 gcacccggct tgctgagcaa gtgctcaacg gctcctgaga agtcgcacta ttgggctgct
      601 gccatatagt ggacatcctt ctcgtgctcg aagactcggg ggaacgagac ctcttacgtg
      661 cccgctttgg aggacgcagg ctacatcctg cgcttgcccg aaatcgagcg ctacctgctg
      721 ctgcgcgacc gcctgcgaga agacgccgag gatcgcaaac tctatgcccg gaccaaacgt
      781 gatctggcga gcagaagctg gccgagcatg gatcactacg ccgaggcgaa gaccgaggtg
      841 gtcgaaggca tcattgccag ggccgctgat gtagggggtg gtagagagcg tgcctctgca
      901 cattgccact ctggaacttt cgagaactcc attagtggaa gttgattgcg tattgcgtac
      961 tacatcgttc ggtgcaacaa ccgtgcagca atacgctgag gaccgttctt caggactacc
     1021 ctttgtaggg acataagcgt gggcgagcga ggacaaaagg gttgcgcgaa ggtttggtta
     1081 tgagcagcat cgaggcgttg atcaagcggt taaagggaac cgccatggtg tctgagacta
     1141 acgtacccac tctaccgagc cacgaccacc ggttctatga gttcgcgggc ggcccgaatg
     1201 gcctctggag ctagagagta gaggtcgggg gagagttcca cgcagcacgc gggattttcg
     1261 gggaagttca cccgccggaa catctcatcg agtggtatgg tccctcttcc cggcggcaag
     1321 tgcaggtctc ccagtccgta gaaggggtgc tcggagaccg gcggttcacc cgtgacgtta
     1381 gttctcatca ggttgtcgtg gaggtggatg tggcgaacga ggggcgctac ggcggcgcat
     1441 tcctcgaaat aatcgaagcc gaagacgccg gccgccaggg ccgcgtgtcc cacgtctagg
     1501 cagaggccga ccgcgggatg gtcaactgcg gagatctgct cggccagttc ggaaggccag
     1561 acggagtagt catagaccac cccgctaata ataagcagtt cagggtagta gttttcgacg
     1621 gcgatggtca cgccgagttc cccggccaga tctcccgcct cccgcagagc atcccgctcg
     1681 gcggcgagct gctcttgcaa actgtaccgg gcgtcgcgcg agcctaaccg ccggcctgca
     1741 tggcacacca ccacgtccgc gccgatgctg ttcgcgaacc agatgctggc cttcaggacg
     1801 tcgcgctgga cgtcgtgggc ggtcagatcc atgaggttca cctcgagcgg ggcgtggacc
     1861 gtgtaggcta gatccgtctt cagcagtcgc tctcgaaccc gctgcgaccg ctccgcgtcg
     1921 aggcttccac cgagtatcac ccccagatgc gccggccaga cctccgcgaa atctggtccg
     1981 gcgatcttta gggcttcgag atcatctgcg agaccggaga cgccctccag aaatagtcgc
     2041 ggcaggttga tgcccacgct ccgaaaagcc atacggttct ttcctcccct agagaccgca
     2101 cgcaaatcgc agcccagtat acggttgggg atcgtctttt gagtaacaga agcgttaaca
     2161 atgggtaaat tctgggttta ctcgattttg tagaataggc tctcgttctc tcgggagaac
     2221 gagagcggga gtagaggctt gggatgagca gttacgagaa ctacacgcag aaggcaggga
     2281 actacgacaa gacgcgggag cccgtgggga ccgagatcat cgtcggatgc ttcgcccacg
     2341 cgccggtgcc gttggaccgg atgagggtgc tggacgccgg gagcggaacc ggtagctact
     2401 cccaggcgtt gctggcttac gtcggtcgga tcgaggcggt ggacctgaac cccggcatgc
     2461 tcgaggtcgc cgcccggaag cttgcggggt cccaggccga gggccggatc aatttctacg
     2521 ccgcgaggat cgacgagctt ccctttgaag actctgcgct cgacggaatc atgatcaacc
     2581 aggtgctgca ccacctgccg gatgaggcct ctaagggatt ccccgcccac cgccgggtct
     2641 tccgcgagtt cgctcgggtc ctgaaacctg gcggggtcct caccgtcaac acttgctcgc
     2701 aagagcaact acgccacggc tactggcact acagcctgat cccggaggcc gccgatgctc
     2761 tccgaaaccg ctacgctcct ctcgacgagc tgatcgggat tctcgacgat tacggcttct
     2821 cccggcgggg aaggttcgtg cctatcgacg ccacagtcca gggtgagtcc tacttcgacc
     2881 cgcgcggccc cctggacaaa gagtggcggg acggcgactc aatctggtct ctggtcaccg
     2941 aagatcgtct cgatcaggct ctctccctgg tccagaagct ggacgagaga ggtgagttgg
     3001 aagcctacgt ggcacgcaac gatgcctggc gccctcacat cggccaggca accatcctat
     3061 tcgcgtcacg aaggtagacg ttttacgaga gtttcacact agagtttgcg cagagtttac
     3121 aatggagaac agggtcgcag actgtgggga agtggccatc gaattttata cggagaggag
     3181 cgaggttgga cgaggtatac tccgcgctga cgcgtatacc cctgttcaag ggcgtgcggc
     3241 cggaagccgt caacctcgag cctctaactt ctctcactaa cgctacctac aaggtaacca
     3301 cgggcggtct agcttacaca ctgcggctcc ccgggaagga cacctacgaa tacgtggacc
     3361 gcgcagccga ggagcacaac gcgcggaaca tggccgccgc cggggtcaac accaaagtac
     3421 tctacttcga cgcgacggac ggcacgatgc tctccgagtt cgtcgaaggg gacaccatga
     3481 acgaggagag gttcaagcgc gattccgaat cgcctgctcg cgtcgccctg gcgctaaagt
     3541 gggttcacca cctggggccg gtcttcaagt cccacttcga cgttttctct atgatcgatg
     3601 tctacctcgg catcctacgc aaactacaag cgtcgctgcc ggaggaatac cacgaggtag
     3661 agcagggagc agaggccgtg cgtcgggtcc tagaggcgtc tcccatgcca ctctcgccct
     3721 gccacaacga cccgtggcct ggcaatctct tggatgcggg cggacgcctc tacatcatcg
     3781 actgggagtt ttccggcatg aatgacccgc tgtgggatct cggcgacctc tcggtcgagg
     3841 ccggatttgg acccaagcaa gaccgggtga tgatggaagc ttactatggc ggcgccgcgc
     3901 ctcaggccct ctatgcacgc ctagcactct acaaggccat gagcgacctc ctctgggccc
     3961 tctggggtct catacagcac gcaaacgaca acccgaggaa cgacttcctg acctacgcgt
     4021 tgggaaggct taggcgctgc aagacgctca tgagcagcac cgacttcggc cgccacctgg
     4081 atgccgtgcg cgtgcgccgt aaagatcttc ctacgctgtc gcaaagctcg ctcggctacc
     4141 tgaacgactc gattctaccc tgatctccaa gctcgttgcc cacccgttac gacttgcgga
     4201 cttgtgcagc aaccgtgcag caacccgagt aaatgacctg aaagctaggg aaaagcgcaa
     4261 tctggaaaat acgtgtgttt gcagctattt tgcacacttc tgaaaggtcc agcaaagtgt
     4321 cgttccgcct ttgcacggcg gaggtcaggg gttcgaatcc cctcggctcc actctaaaaa
     4381 gttgttgatt tgcagacaaa atatgcgaaa tgtggagaca aattagaaaa gtcgcggctc
     4441 cctt
//
LOCUS       DASTUV010000493         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_980636, whole genome shotgun sequence.
ACCESSION   DASTUV010000493 DASTUV010000000
VERSION     DASTUV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980636"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFT03_09955"
     CDS             <1..801
                     /locus_tag="VFT03_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEU4848512.1"
                     /translation="SREDELKGTAGGVKRLASRFDDTFVVVSGDALTDVDIRELVAFH
                     KEKDALATIALHRVYDTSEFGVVDVDEDGNILGFQEKPAPEEAISTLANTGIYVLEPR
                     ALEYVPEDAFFDFAKDVFPRYLENGERFVGYQGDFYWSDIGTLEAYRQAQYDVLSGKV
                     KVEIPGEKRGESLWVGEDAQIHPSASIEGHVLVGRDAVVGRGVTLSGDVTVGSDCWIR
                     PGATIKRSILLPGAMVGDGAYLEDCIVGHGYDVRPGETIRGGALIRRA"
     gene            complement(830..1519)
                     /locus_tag="VFT03_09960"
     CDS             complement(830..1519)
                     /locus_tag="VFT03_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848513.1"
                     /translation="MNGSVLLVIGDDEERLHLRRALSGEGWRVIPAGSGSQARQMADR
                     QEFEVAVVDNRLPDERGFQVISAVQKPGVSTVALLHDEDTMDRIVGIELGADYYMRSP
                     VNPRELVARVKQIFRKRELVGNGPSGKIYVGELEIDPDQVQVRKGDREILLSPREFRL
                     LYTLARSPGRVFPRDALLRQVWGEDEYIDERTVNVYVQRLRNKLGDNPTDPKLIETVR
                     GFGYRLVRPKE"
     gene            1647..1720
                     /locus_tag="VFT03_09965"
     tRNA            1647..1720
                     /locus_tag="VFT03_09965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1681..1683,aa:Arg,seq:acg)
     gene            complement(1820..>2108)
                     /locus_tag="VFT03_09970"
     CDS             complement(1820..>2108)
                     /locus_tag="VFT03_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU4848514.1"
                     /translation="LVFPSKTGTPMSANNLSRRHFKPLLERAGLPDVRLYDLRHTFAT
                     LWLESGEHPKILQEILGHSRISVTLDTYSHVVPHMQREAMGRFGERFTASS"
BASE COUNT          389 a          671 c          660 g          388 t
ORIGIN      
        1 agccgggagg acgagctaaa gggcacggcc ggcggcgtca agcgcctggc gagcaggttc
       61 gacgacacct tcgtcgtcgt ctccggcgac gccctcaccg acgtcgacat cagggagctg
      121 gtcgccttcc acaaagagaa agacgctctg gcgaccatcg cgcttcaccg ggtctacgat
      181 acctccgagt tcggggtcgt cgatgtggac gaggatggga acatccttgg attccaggag
      241 aagcccgcgc ccgaggaggc gatcagcact ctagccaaca ccggcatcta cgtgctggag
      301 ccgagggctc tggagtacgt accagaggac gccttcttcg acttcgccaa ggacgtcttc
      361 ccgcggtacc tggagaacgg ggagcgcttc gtcggctacc agggagactt ttactggtcg
      421 gacatcggga cgctcgaggc ttaccggcag gcccagtacg acgtgctctc aggaaaggta
      481 aaggtcgaga tcccgggcga gaagcgcggg gagagcctgt gggtcgggga ggacgctcag
      541 attcacccct cggccagtat cgagggccac gtgctcgtgg ggcgagacgc ggtcgtgggg
      601 cgcggtgtga cgctctccgg agacgtgacg gtggggagcg actgctggat ccgtcccggc
      661 gccacgataa agcggagcat cctgctcccc ggcgcgatgg tgggcgacgg cgcctacctc
      721 gaagattgca tcgtgggcca cggctacgac gtgcgtccag gcgagacgat ccggggcggc
      781 gccttgatac gccgcgcata aagacgcagc atgctacgcc tctacgtctt tactccttcg
      841 gcctcacgag gcggtagccg aagcccctta cggtctctat gagcttgggg tctgtcgggt
      901 tgtcgccgag cttgtttcgg aggcgctgga cgtagacgtt cacggtacgc tcgtctatgt
      961 attcgtcctc gccccagacc tgccgcagca gagcgtcgcg cgggaagacc cgtcccggac
     1021 tgcgggcgag cgtatagagc agcctgaact cgcggggaga gagcaggatc tccctgtccc
     1081 ccttcctgac ctgtacctgg tcgggatcta tctccagctc cccgacgtag atcttgccgg
     1141 aaggtccgtt cccgacgagc tcgcgcttgc ggaatatctg tttgacgcgc gccaccaact
     1201 ctctcgggtt gaccggcgag cgcatgtagt agtccgcccc gagctctatg ccgactatgc
     1261 ggtccatagt gtcctcgtcg tgcaagagcg ccacggtcga gacgccaggc ttctggacgg
     1321 ctgagatcac ctggaagccc ctctcgtccg gcagccggtt gtccacgacg gccacctcga
     1381 attcctgccg gtcggccatc tgccgcgcct gcgagcccga gccggccggg atcacacgcc
     1441 atccctcgcc ggagagagcg cgcctgagat gcaaccgctc ttcatcatct cctatgacca
     1501 gcagcacgga cccgttcaag tcgctacgcc cacctctatc cgcaaacgct cgcctctacc
     1561 actagaaagt atatccacaa atccctccca gagggacccc gaccttgaga gatgcccctc
     1621 tcggcggtat catatgccct cccgatgcgc tcgtagctca gccggataga gcgtctgact
     1681 acgaatcaga aggtcgcagg ttcgagtcct gccgagcgcg ctcccaaaag tcctgcaaat
     1741 gcagtgtttt ctcgttgtgt cttgacgcct actttcccgg gttgaccgat ttaccacaac
     1801 tactaccaca accagagcct caagatgacg ccgtaaaccg ctcgccgaag cgtcccatcg
     1861 cctcgcgctg catgtggggc acaacgtggc tgtaggtatc gagcgtgacg gagattcgtg
     1921 aatggccaag gatctcctgc aggatcttcg ggtgctcccc gctctcgagc cagagcgtgg
     1981 cgaaggtgtg gcggaggtcg tagaggcgca catctggcag accagcccgc tcgagtagcg
     2041 gcttgaagtg ccgccgacta aggttgttgg cgctcatcgg ggtgcctgtc ttcgacggga
     2101 aaacgagg
//
LOCUS       DASTUV010000494         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_427804, whole genome shotgun sequence.
ACCESSION   DASTUV010000494 DASTUV010000000
VERSION     DASTUV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427804"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFT03_09975"
     CDS             complement(<1..653)
                     /locus_tag="VFT03_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU4848515.1"
                     /translation="MAETQVLQKVLRDDQRLPRVPEPAVMVIFGASGDLTRRKLIPAL
                     YDLAAQRRLPMELAVVGISRTEMSHEDFRARLHDALKEQRPGRVSEDVWESFSRGIFY
                     LPGDSKKPETYEELKSFLKQLDDERGTEGNRIYYLSSSPSLFPTIVERLGEAGMNRGE
                     NGGYARLVVEKPFGRDLMSARELNQEIRRYFEEHQIYRIDHYLGKDTVQNILALRFA"
     gene            complement(665..1582)
                     /gene="gnd"
                     /locus_tag="VFT03_09980"
     CDS             complement(665..1582)
                     /gene="gnd"
                     /locus_tag="VFT03_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU4848516.1"
                     /translation="MDIGIWGLGRMGFNMARRLVDSDEHRVVAGNRSPGKVDDAVEQG
                     AESAYSVEEMVEKLNPPRVLWSMLPAGDVTDEMIDRFVELADEGDIIIDGANSYYKDS
                     IRRAEKVRSEGLKWLDAGVSGGIWGYEVGYCTMVGGDADAFEYAEPVFKTLAPPDGYA
                     YLGDAGAGHFAKMVHNGVEYGMMQAYAEGFEILKKSRYDYDLRAICSLWNRGSVVRSW
                     LLELAERAFEKDADLHHIQGYVEDSGEGRWTVMEAIDEDVAANVIAASLFARIASREE
                     DAFALKVLAALRGQFGGHAIKEAATESEK"
     gene            complement(1655..2749)
                     /gene="tal"
                     /locus_tag="VFT03_09985"
     CDS             complement(1655..2749)
                     /gene="tal"
                     /locus_tag="VFT03_09985"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU4848517.1"
                     /translation="MNERLRKMHELGQSPWVDEISRDDIKNGGLERLIEDGILGATSN
                     PAIFQKAIAGSELYDEQLQELARKEDDPKEIFWGIARTDITDACDIFMPVYERTGGED
                     GYVSLEVQPDIAYDTEATIEEAARLHDMIDRPNLFVKIPATLQGLVAIEEMISRGKSI
                     NVTLIFSLERYREVVRAYIRGVKRLVESGGDPSDVRSVASFFVSRIDSEADKRLEELG
                     ADDLKGRLAIANAKIAYQIYKQVFGGSRWRSLEERGANRQRLLWASTSTKNPDYPDTL
                     YVDNLVGPQTVNTMPKQTIEAVKDHGNIRPTLEEGVEEAKELLERLREAGLDYEDVTD
                     TLEQEGIQKFADPFHALLEEIERKSKQLVS"
BASE COUNT          470 a          991 c          887 g          614 t
ORIGIN      
        1 ttggcgaacc gtaaggccag gatgttctga acggtgtcct tgccgaggta gtggtctatg
       61 cggtagatct ggtgttcctc gaaatagcgc cggatctctt gattcaactc cctggcgctc
      121 atcaggtccc gcccgaacgg cttctcgacc acgagccggg cgtagccgcc gttctccccc
      181 ctgttcatcc ccgcctcccc gagcctctct acgatcgtcg ggaagagtga gggggaagag
      241 gagaggtagt agatccggtt cccctcggtc ccacgctcgt cgtcgagctg cttcaagaag
      301 ctcttgagct cctcgtaggt ctcgggtttc ttagagtcgc cggggaggta gaagatccct
      361 ctggagaacg actcccagac gtcctccgag acccggccag ggcgctgctc cttcagggca
      421 tcgtgcaacc gcgcgcggaa atcctcgtgg ctcatctcgg tgcgggagat accgacgacg
      481 gcgagctcca tcggcagccg cctctgggcg gcaaggtcgt agagcgcggg gatcagcttc
      541 cggcgcgtca ggtcgcccga cgcgccgaag atgaccatta ccgccggctc agggacccgc
      601 ggcagccgct gatcgtcccg aagaactttc tgtagtacct gcgtctcggc cacgagccgt
      661 cctcctactt ctcgctctct gtggccgcct ccttgatcgc gtgcccgccg aactggccgc
      721 gcagcgccgc gaggactttg agggcaaacg catcttcctc gcgcgaggca atgcgggcga
      781 agagcgaggc cgcgatcacg ttcgccgcca cgtcctcatc tatggcctcc attaccgtcc
      841 agcgtccctc acccgagtcc tcgacgtagc cctggatgtg atgcaggtcg gcgtccttct
      901 cgaaagccct ctcggcgagc tcgagcagcc agctgcgcac cacgctcccc cgattccaga
      961 ggctgcagat ggcgcgcagg tcgtagtcgt agcgggactt cttgaggatc tcgaagccct
     1021 cggcgtaggc ctgcatcatc ccgtactcga cgccgttgtg gaccatcttt gcgaaatgcc
     1081 cggctccggc gtccccgagg taagcgtagc cgtccggggg cgcgagcgtc ttgaagaccg
     1141 gctcggcgta ctcgaaggcg tcggcgtcgc ccccgaccat ggtgcagtag cctacctcgt
     1201 agccccagat cccccccgag acgcccgcgt ccagccactt tagaccctca ctcctgacct
     1261 tctcggcgcg tcggatggag tccttgtagt aggagttggc cccgtcgatg atgatgtcgc
     1321 cctcgtcggc cagctcgacg aaccgatcta tcatctcatc ggtcacgtca ccggccggga
     1381 gcatgctcca cagcaccctc ggagggttga gcttttcgac catctcctcc acgctgtagg
     1441 cgctctcggc tccctgctcg accgcgtcgt caactttgcc cggcgagcgg ttgccggcta
     1501 cgacacggtg ctcgtcactg tccacgagcc ggcgcgccat gttgaagccc atccgaccca
     1561 ggccccagat accgatgtcc attttcgctc ccttcggtcg ctcgggcttc aggcatcagg
     1621 tttcaggcat caggaaatac ctggagcccg aagcctaact tactagttgc ttgctcttgc
     1681 gttctatctc ctcgagtagt gcgtggaagg ggtcggcgaa cttctggatg ccctcctgtt
     1741 ccagggtgtc cgtcacgtct tcgtagtcca ggcccgcttc gcggagacgc tcgagaagct
     1801 ccttggcctc ctcgacgcct tcctcgaggg tgggtcggat gtttccgtgg tccttgaccg
     1861 cttctatcgt ctgctttggc atggtgttca ccgtctgggg cccgacgagg ttgtccacat
     1921 agagggtgtc ggggtaatcg gggttcttgg tcgaggtcga agcccacagg agacgctgcc
     1981 tgttggcgcc tctttcctcg agggaccgcc accgggagcc gccgaatact tgcttgtaga
     2041 tctgataggc gatcttggcg ttggctatgg cgagcctgcc cttgagatcg tcagccccga
     2101 gctcctccaa ccgcttatcc gcctccgagt ctatcctgga gacgaagaag ctcgccaccg
     2161 agcgcacgtc cgaggggtct cccccgctct cgacgagcct cttgacgccc cggatgtagg
     2221 cacggaccac ctcgcggtag cgctccaggg agaagatgag ggtgacgttt atggacttgc
     2281 cgcgggagat catctcctca atggccacca gcccctgcaa cgtggccggt atcttgacaa
     2341 agaggttggg cctgtcgatc atgtcgtgca gcctcgcggc ctcctcgatc gtagcctccg
     2401 tgtcgtaggc gatgtctggc tggacctcga gggagacgta gccgtcctcg ccacccgtcc
     2461 tctcgtagac gggcatgaag atgtcgcagg cgtccgtgat gtcggtccgg gctatgcccc
     2521 agaagatctc cttggggtca tcctccttcc gggcgagctc ctggagctgc tcgtcgtaga
     2581 gctcgctccc tgctatggcc ttctggaaga tggcggggtt ggaggtcgcg ccaaggatac
     2641 cgtcctctat cagccgctcg agcccgccgt tctttatgtc gtcgcgggag atctcgtcca
     2701 cccagggcga ctgccccagc tcgtgcatct ttcgcaaacg ctcgttcacg ggtacctcct
     2761 tgtgcggccg agagccagcc tttcccgtca gtctacaccc gacccgcgcg gcgggacgga
     2821 ggaactcccg gaactctcca cagccgcccg tcgcaccatc cgatgcgagg ggacttacca
     2881 ctttgagtca tggatcaaac cccgagcacg taaggagccg gggcaagatc ttgccccggc
     2941 tccttacgtg ctcggggttt ga
//
LOCUS       DASTUV010000495         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1337346, whole genome shotgun sequence.
ACCESSION   DASTUV010000495 DASTUV010000000
VERSION     DASTUV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337346"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFT03_09990"
     CDS             complement(<1..919)
                     /locus_tag="VFT03_09990"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEU4848518.1"
                     /translation="MLVKDELLDRVVARVREQLAEDQAPQVEEFARQYYAWITAEDLA
                     DRSPIDLYGAAVGHWTFARQRAPDEPKVRVYNPQFDEHGWQSTHTVIEIVTDDMPFLV
                     DSARMGVNREGYAIHLMLHPIMKVRRDDAGRLVEVLEPDSDDEEGVLNESVIHLEIDR
                     QTEPEVLNGLHQCITTVLQQVRAAVEDWPDMRERVREFVSEIDENRPPVEDEDPVEAR
                     AFLEWISDDNFTFLGYREYDLLTEDGEDLLRSVPGTGLGILRQTDQKPHSLSFAKLPH
                     EVRRLAHSPDILTLTKANSRATVHRPSYLD"
     gene            1068..2192
                     /locus_tag="VFT03_09995"
     CDS             1068..2192
                     /locus_tag="VFT03_09995"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU4848519.1"
                     /translation="MPVEKEDYTLGVEEEYQVIDPETRALCPGAEDVLRRARRTLGED
                     RVVPELRASQLEVVTPVCRTLSEVRGELLRLRWGVIRAAEEAGVRIAAASTHPFSHWQ
                     DQPITAGERYEKILDRERRMAEEQVVFGFHVHVGLSDREAAVQTMNRARLWLAPLLAL
                     SANSPFWMGEDTGYASYRTQIWGWLPTAGPPGPFSSLAEHDALVESLVATGGAMEANQ
                     VYWDMRLPQKLETVEIRVCDVCSSIDEAVMLAGLCRALVRTCHKGAEEEKPYPEVRPE
                     ILRAAHWVASRYGLGADLVDVEAGHLAPAKEVIEKLLAFTRRALEEYGDWKEVSVLVG
                     DTLKRGNGAVRQCQVYERSGKLGDVVDALIEDTAKGTNAS"
     gene            2446..3621
                     /locus_tag="VFT03_10000"
     CDS             2446..3621
                     /locus_tag="VFT03_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4848520.1"
                     /translation="MNFELSAEQQEIHERAAEFADRELAHYAADLDREDRVPFETLEK
                     LSEMGFMGLCVPEEYGGAGADFLSYVLLIEEISRADAGVGVTLAVHTSAGTLPIVMYG
                     DEDQKARWVPDLAQGNKIGSFALTEPTTGSDASAIQTTAERVEGGYRLSGHKQWVTNG
                     RVAGSMISFARAPGGVTAFVVSMDAEGVSFGKHAQKMGVISATTDDVLLDNVFVPEED
                     RLLPEGKGLSVALGTLDFGRIGIAAQALGIAEAAFLYASEYAAQRTTFGKPIAEHQAI
                     AFKLADMQTKIRAARLLTHEAAWMKDRGMRVTEAGARAKLYASQVANEVTYDAVQILG
                     GYGYMKDHPVERHYRDARVTEIYEGTSEIQRLVISRGILKERAESPGGVEGSRPVVG"
     gene            3763..>3954
                     /locus_tag="VFT03_10005"
     CDS             3763..>3954
                     /locus_tag="VFT03_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU4848521.1"
                     /translation="MDTKKGARERWEEAYSKNPERDAEFSTMSGIPIKPLYTPDDVEG
                     SYDEKLGYPGEYPYTRGVYP"
BASE COUNT          735 a         1223 c         1304 g          692 t
ORIGIN      
        1 agtcgagata ggaaggccgg tgcacggtcg cccgcgagtt cgccttggtc agggtgagga
       61 tgtcgggcga gtgggcaagc cttcgcacct cgtgcggcag cttggcgaag ctgagcgagt
      121 ggggcttctg gtccgtctgc cgtagtatgc cgagacccgt ccccggcacg gaccttagca
      181 ggtcctcccc atcctcggtg agcagatcgt actcgcggta gcccaggaaa gtgaagttgt
      241 cgtcggagat ccactcgagg aaagccctgg cctccaccgg atcttcatcc tccacgggcg
      301 gccggttttc gtctatctcc gagacgaact cgcgcaccct ctcgcgcatg tcgggccagt
      361 cctcgacggc ggcgcgcacc tgctgcagga cggtggtgat gcactggtgg agcccattta
      421 gcacctcggg ctcggtctgc cggtctatct ccaggtggat gaccgactcg ttgagaacgc
      481 cttcctcgtc gtcggagtcg ggttcgagga cctcgacgag cctgcctgca tcatcccgcc
      541 tcaccttcat tatggggtgg agcatgaggt ggatcgcgta cccttcgcgg tttactccca
      601 tcctggccga atccaccagg aacggcatgt cgtccgtgac gatctcgata acggtgtggg
      661 tggactgcca gccgtgctcg tcgaactgcg ggttgtaaac gcggacttta ggctcgtcgg
      721 gcgctcgctg tctcgcgaac gtccagtgac ccacggcggc cccatacagg tcgatggggg
      781 aacggtccgc caggtcctcg gcggtgatcc aggcgtagta ctgacgcgcg aactcctcga
      841 cctgcggcgc ctgatcctcg gccaactgct cccgaacgcg agcaacgacc ctgtcgagaa
      901 gctcgtcctt gactaacatc ggcacgctcc tccgttacat ttccctctcc caaataatac
      961 tccctgttct catccggtgc gtacatcccg gcaggctttc tcgttcccgt ctggtcggaa
     1021 taatggcgcc gtgttacctt tcggtacagc gaaggaggtg agaccgggtg ccagtggaga
     1081 aggaagatta caccctcggg gtcgaggagg aataccaggt catcgaccct gagacccgcg
     1141 ctctctgccc cggcgccgag gacgtgctgc gtcgggcgcg gcgaactcta ggcgaagacc
     1201 gggttgtgcc cgagcttcgc gcctcgcagc tcgaggtcgt cacccccgta tgtcgcacgc
     1261 tttcggaggt gcgcggcgag cttttgcggc tgcggtgggg tgtgatccgg gccgccgaag
     1321 aagcgggcgt acgtatcgcc gccgcgagca cccacccgtt ctcgcactgg caagaccagc
     1381 ccataaccgc cggggaacgc tacgaaaaga tcctcgaccg cgagcgccgg atggcagagg
     1441 agcaggtcgt cttcggcttc cacgtccacg tcggcttgag cgaccgcgag gcagccgttc
     1501 agaccatgaa ccgggcacgc ctctggctcg cgccgctgct cgcgctctcg gccaattccc
     1561 ctttctggat gggtgaggac accggctacg caagctatcg cacccagatc tggggctggc
     1621 tcccgaccgc cggcccaccc ggacccttct cctcgctagc cgagcacgac gccctggtag
     1681 agagcctcgt cgcgacgggc ggcgcgatgg aggcgaacca ggtctactgg gacatgaggc
     1741 tcccgcagaa gctggagacc gtcgagatcc gggtctgcga cgtctgcagc agcatagacg
     1801 aggccgtgat gctcgccggc ctctgccgcg ctctcgtacg cacctgccac aaaggagccg
     1861 aagaggagaa gccgtacccc gaggtgcgcc ccgagatcct gcgcgcggcg cactgggtcg
     1921 cctcgcgcta cgggctgggc gccgatctcg tagacgtcga agccgggcat ctcgccccgg
     1981 cgaaggaggt tatcgagaag ctgctggcct tcacgcgccg ggcgctcgag gagtacgggg
     2041 actggaaaga ggtgtctgtg ctggtgggcg acaccctgaa aaggggtaac ggggctgtgc
     2101 gccagtgtca ggtctacgag cgatcgggaa agctcggaga cgtcgttgac gcgctaatcg
     2161 aggacacggc gaaggggact aacgcgtcgt aggttcgggc tgattcttcg acacgtttcg
     2221 agtatgcggc aaggagtacg agcttgaatt tgtccgaaaa ggatgcatct gcttcgggaa
     2281 ctatgggtga gtagtgacga gatgccgatg ctccggaagt cgtagtttct cttaaagact
     2341 tctcgcttcc ccatgctaca aatttaacgg agaggttgtc gcgactcgag taatcagaag
     2401 gtatcttgtt tacgctgcaa tggtaaaggc aagaatggag acgttgtgaa cttcgagctc
     2461 agcgcagagc agcaggaaat ccacgagcgg gccgcggagt tcgcggaccg ggagctggcg
     2521 cattacgctg cggacctgga tcgtgaagac cgggtcccct tcgagacgct ggagaagctc
     2581 tcggagatgg gcttcatggg gctgtgcgta ccggaggagt acggcggggc cggggcggac
     2641 ttcctctcat acgtgttgct gatcgaggag atcagccgcg ccgacgctgg tgtcggcgtg
     2701 acgctcgctg tccacactag cgcgggcacg ctcccgatcg tgatgtacgg ggatgaggac
     2761 cagaaggcga ggtgggtgcc ggatcttgct cagggaaaca agatcgggtc cttcgccctc
     2821 accgagccca ctacaggctc cgacgcgtcc gcgatacaga cgaccgccga gcgggtcgaa
     2881 ggcggctaca ggctctcggg ccacaagcag tgggttacga acggccgggt cgccggctcc
     2941 atgatctcct tcgcccgcgc ccccgggggc gtgacggctt tcgtagtctc catggacgcc
     3001 gaaggcgtct cgtttggcaa gcacgcgcag aagatgggcg tcatctccgc cacgaccgac
     3061 gacgtgcttc tggacaacgt cttcgtcccg gaggaagacc ggctcctgcc cgagggcaag
     3121 gggctttccg tagcgctcgg aacgctggac ttcggcagga tcgggatcgc cgcgcaggcc
     3181 cttggtatcg ccgaagccgc cttcctgtac gcctcggaat acgcggcaca gaggacgacc
     3241 ttcggcaagc ccatagccga gcaccaggcc atagcgttca agctggcgga catgcagacc
     3301 aagataaggg ccgcgcggct cctcacccac gaggcagcct ggatgaagga ccgcgggatg
     3361 cgcgttaccg aagccggcgc ccgcgcgaag ctctacgcct cgcaggtggc taacgaggtc
     3421 acctacgacg cggttcagat tctaggcggc tacgggtaca tgaaggatca ccctgtcgag
     3481 cgtcactacc gcgacgcgcg ggtcacggag atctacgagg gcacgagcga gatacagcgc
     3541 ctcgtcatct cgcgcggcat cctcaaggag agagcagagt ctccgggcgg cgtcgagggc
     3601 tcccggccag tcgtcgggta ggaagatcgg tgatcgtccc cgcaatgaac gagagtgaga
     3661 cggtcgagag cgcgcagggg gctggatctc accggccgcc ttggaattcg ggggttatgc
     3721 tacagtctag gcaaagggag cggacacacc ggagggtata aagtggacac caaaaagggc
     3781 gcgcgcgagc gctgggagga agcctactcg aagaaccccg agcgcgacgc cgagttctcc
     3841 accatgtccg gcatccccat caaacccctc tacacccccg atgacgtgga aggctcctac
     3901 gacgagaagc tgggttatcc cggcgagtac ccttacacgc gcggcgtcta cccg
//
LOCUS       DASTUV010000496         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1448222, whole genome shotgun sequence.
ACCESSION   DASTUV010000496 DASTUV010000000
VERSION     DASTUV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448222"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFT03_10010"
     CDS             <1..889
                     /locus_tag="VFT03_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="HEU4848522.1"
                     /translation="ANDPTTLERFWYVVSGGNLRGGFFAFGPAELPGRLLYYLGHLLD
                     NFHLGLVAIGVAGFVVLYLRDRVVAVFLSFLYFGWLFHAIENDIPDIHLYFIPTYLVL
                     CIAMAVGFTLLHSEVQSILERFERVPKKVAVGAVSVTLLLLPLVGVWDTYANNDMSGD
                     YRGRQILEDVAENAERNATILHHRSNLYYLVLVEKRRQDLTLVDPFWHNRDIGYADIV
                     WPADVDLGEANRLYGTDDFSGVTAAEKAAEKGPVYIINQEDIDPSGLYEAGWHTTQVE
                     GALFRLVPPDGEAAGGDGR"
     gene            complement(903..2522)
                     /locus_tag="VFT03_10015"
     CDS             complement(903..2522)
                     /locus_tag="VFT03_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU4848523.1"
                     /translation="MDQPRRLLLSGKTCAALVLLAACLLVGCGPSSKGEVADVGDGAD
                     SGTATSDEPAERPNIILILTDDLDDDDLSYMPNLRSLLVEQGTTFENAFVTDSLCCPS
                     RATILRGQYAHNHGILENGSPNGGYEKFRSLGRQNSTLATWLQDAGYRTVLIGKYMND
                     YEGTQVPPGWDDWHAVSGNYTSHDLSENGKIVSYDPGSYYMTDVLADIAVDFIHDPDG
                     RVPSFLVPSRPFFMWLGTTAPHQPADPAPRHANAFSDVSMPRPPSFDEGDVSDKPAWI
                     RDNPPLDPEQIAYAEDLYRKRMQSMLAVDDMIGRLVGELQKSGELDNTYFFFTSDNGF
                     HFGTHRLSVGKWTAYEEDISVPLIVRGPGVPKGRTLEHLVLNNDFAPTFADLGGAEIP
                     DFVDGRTLRPLLSDDPPPTDDWRQAFLEEAMAESAVAPASVTEEAGPRLLTGDVQPSE
                     EVGRASPLQKSSVKEAGRPALKAVRTRDHLYVEYDNGESELYDLEKDPYELDNVYEDT
                     DLDQLWRYEGWLGSLRDCAGEDCRAAEDAGP"
     gene            2627..>3146
                     /locus_tag="VFT03_10020"
     CDS             2627..>3146
                     /locus_tag="VFT03_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848524.1"
                     /translation="MLHDLIRVLPVALLVCFLPGWFWARLLSTATDRAEQAAYSVALA
                     ITLVPTAALVMARLFGTGVTLAIAVAAPLLVFTAGLVAYLRFGAAKGSDVPLAPPPAP
                     LGTPALALLAAALSLALVGFFDLVPGAWAAILVAPLVLLAGIVHLLTSSRYDSSRLDA
                     LSAAFTPAVRYAV"
BASE COUNT          488 a         1037 c         1006 g          615 t
ORIGIN      
        1 ggctaacgac ccgacgacct tggagcgttt ctggtacgtg gtcagcggag gcaacctgcg
       61 cggcgggttc tttgctttcg ggccggcgga gctgcctggc cggctgttgt actacctggg
      121 ccacctcctc gacaacttcc acctcgggct ggtggccatc ggtgtggccg gcttcgtagt
      181 cctctacctg cgggaccgcg tggtcgccgt gttcctgagc tttctgtact tcggctggct
      241 cttccacgcc atcgagaacg acatcccgga catccacctg tactttatcc ctacctacct
      301 ggtgctctgc atcgcgatgg ccgtcggatt cacgcttctg cactcggagg tgcagagcat
      361 cctcgagagg ttcgagcggg ttcccaaaaa ggtggctgtg ggcgctgtct ccgtgacgct
      421 gctgcttttg cctctagttg gcgtctggga cacctacgcg aacaacgaca tgagcgggga
      481 ctacagggga cgccagatcc tggaagacgt ggccgagaac gcagagcgga acgcgaccat
      541 tctgcaccac aggagcaacc tctattacct cgtgctggtc gagaagcgca ggcaggatct
      601 gacactagtt gaccccttct ggcacaacag ggacatcggc tacgccgaca tcgtctggcc
      661 tgccgacgtc gacctcgggg aggcgaaccg tctctacggc accgacgact tctccggggt
      721 caccgccgct gagaaggcgg cggagaaagg ccccgtatac atcatcaacc aggaggacat
      781 agaccccagc ggcctgtacg aggcggggtg gcacaccact caggtcgaag gcgctctttt
      841 caggctggtc ccgcccgacg gtgaggccgc cgggggagac ggccgctaga ggtaggcagg
      901 cttcagggtc ctgcgtcctc ggcggcgcgg cagtcctccc cggcgcaatc gcgcagcgac
      961 cctagccacc cctcgtaccg ccagagctgg tcgaggtcgg tatcctcgta gacgttgtcg
     1021 agctcgtagg gatccttctc caggtcgtag agctcgctct ccccgttatc gtactccacg
     1081 tagaggtggt cccgggtcct cactgccttc agggctgggc gtcccgcctc cttgactgag
     1141 gacttctgca agggagaagc tcgtcccacc tcctccgagg gctgcacgtc gccggtcagc
     1201 agccgcgggc ccgcctcttc ggttacggag gcgggcgcca cggcagactc cgccatggcc
     1261 tcctcgagga aagcctgccg ccagtcgtcc gtgggaggcg ggtcgtcgct caaaaggggc
     1321 ctcagcgtcc ggccgtccac gaagtccggg atctcggccc cgcccagatc ggcgaaggtt
     1381 ggcgcgaagt cgttgttcag taccaggtgc tccagcgtcc ggcccttagg cacccctggg
     1441 ccgcgcacga tcaggggcac gctgatgtcc tcttcgtagg ctgtccactt gcccacgctc
     1501 aggcggtggg tcccgaagtg gaagccgttg tccgaggtga agaagaagta ggtgttgtcc
     1561 agctctccgc tcttttgcag ctcgcccacc aggcgcccta tcatgtcgtc cacggcgagc
     1621 atcgactgca tgcgcttgcg gtagagatct tcggcgtagg cgatctgctc cgggtccaga
     1681 ggcggattgt cgcgtatcca ggccggtttg tcggagacgt cgccttcgtc gaaggatgga
     1741 gggcgcggca tcgagacatc agagaacgcg ttcgcgtgac ggggggccgg gtctgcgggc
     1801 tggtgtggcg ccgtagtgcc gagccacatg aagaagggtc gactcggcac caggaaagac
     1861 gggacgcgcc cgtccgggtc gtggatgaag tcgacggcta tgtcggcgag aacgtccgtc
     1921 atgtagtaac tccccgggtc gtagctgacg atcttgccgt tctcgcttag atcgtggctc
     1981 gtgtagttgc cggacaccgc gtgccaatcg tcccacccgg gcggcacctg ggtcccctcg
     2041 tagtcgttca tgtacttgcc gatcaacacc gtccggtagc ccgcgtcttg cagccaggtg
     2101 gccagcgtcg agttctggcg gcccagagag cggaacttct cgtagccgcc gttcggtgac
     2161 ccgttctcga ggatcccgtg gttatgggcg tactggccgc gcaggatcgt ggcccggctc
     2221 gggcagcaaa gcgagtccgt tacgaaggcg ttctcgaagg tggtgccctg ctcgacgagc
     2281 agggaccgca agttcggcat atacgagagg tcatcgtcgt ccagatcgtc ggtgaggatc
     2341 aggatgatgt tgggccgctc cgcaggctcg tcgctcgtcg ccgtcccgga atcggccccg
     2401 tccccgacgt ccgcgacctc ccctttgctc gaaggaccac acccgaccag caggcatgcg
     2461 gcgagcaata cgagggccgc gcacgtcttt cccgataaca ggagccttct tggctgatcc
     2521 acggcggcca ttatattcgc cggccggccg attgtttaat ctgggctaac tcctgctata
     2581 cactctcggg cgtgaaagta tcgaccttca acgagataga cccggcgtgc tccacgatct
     2641 gatccgggtc ctccccgtgg cgctcctggt gtgcttcttg ccgggatggt tctgggcgcg
     2701 gctcctgagc accgcaacag accgcgccga gcaggccgcc tactcggtag ccctcgccat
     2761 cacgctggtg ccgacagccg cgctagtaat ggcgcgcctc tttggcacag gcgtgaccct
     2821 ggccatagcc gtcgcagccc cgctcctcgt attcacggcg ggccttgtag catatctacg
     2881 ctttggagca gccaagggct ctgacgtccc tctcgcccct ccgcccgctc cactgggcac
     2941 gccagctctc gcgctcctcg ccgcggcgtt gtctctcgcg ctagtggggt tcttcgacct
     3001 cgtccccggc gcgtgggcgg cgatcctcgt cgcgccgctg gtgctcctgg cgggtatcgt
     3061 ccatctgctg acgtcctccc ggtacgactc gtcgcggctg gatgcactgt ccgcggcgtt
     3121 taccccggca gtgcgctacg ccgtcg
//
LOCUS       DASTUV010000497         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_901657, whole genome shotgun sequence.
ACCESSION   DASTUV010000497 DASTUV010000000
VERSION     DASTUV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901657"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VFT03_10025"
     CDS             complement(<1..1060)
                     /locus_tag="VFT03_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU4848525.1"
                     /translation="MRAGGAAVRRRIRERPRIRPKVYGYLTEEKESLRQGFAALFLSS
                     TGELVAGIALAGIAGILDELVGLAVLIPAAIGMRGAIFGAMGSRLSTAIQTGLFSFNL
                     RRGVLAENVQAAAILSLISGVFLAFAARLLCEFLGVRTELSIFDYVVISTIGGMIAGV
                     LLLGITVVVARLSVEREWDMDNIAAPMITAFGDILTLPALVVATYLIGIPVFSNILAG
                     ALIVVAVAVAVTAFRIGPPSLRRILAESLPILALTGMVTILVGVALEDRMEAFVALPA
                     LFILLPAFLQEGGALGGMLSSRLSSKIHLGLLEPRGLPPVDTFRDFTLIYTFATGVYL
                     FIGGASHWLAVALGTLSPG"
     gene            1390..>2306
                     /locus_tag="VFT03_10030"
     CDS             1390..>2306
                     /locus_tag="VFT03_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEU4848526.1"
                     /translation="MMIVDNAIYVDGRRTVEPHTVRETHEAVRDTHGLAWIGLYKPTE
                     EEFAAVAGEFGLHELAVEDAIKAHQRPKIERYGDTLFVVLKPARYLDAPEEVEFGEVH
                     VFVGRDFVITVRHGEAPALAEVRSRLEGEPDLLRLGPEAILYAIMDRVVDGYAPVVDG
                     LENDIDEIEVEVFSGNPGVTRRIYELSREVIEFQRATKPLTGMLARLIAGFEKYDVDT
                     ELQRYLRDVQDHVVQVVERVEGFRELLQNILSVNLTLVSVQQNEEVKSLTGASIDQNE
                     EVKKISAWAAILFAPTLIGTVYGMNFDYMP"
BASE COUNT          461 a          689 c          784 g          372 t
ORIGIN      
        1 ggccggggct cagggtgccg agcgctacgg ccagccagtg ggacgcaccc cctatgaaca
       61 ggtacacgcc ggtcgcgaag gtgtagatca gggtgaagtc cctgaaggtg tcgacgggtg
      121 ggagcccccg gggctcgagg agaccgaggt gtatcttgga ggagagccgg ctggagagca
      181 tgccgcccag agccccgccc tcctgcagga aagccggcag gaggatgaac agcgcgggta
      241 gcgctacaaa ggcctccatc cggtcttcga gggcgacgcc tacgaggatg gtcaccatcc
      301 ctgtgagcgc cagtatcggg aggctctcgg ccaggatacg cctgagggag ggtgggccaa
      361 tccggaaggc ggtcaccgcg accgcgacgg cgacgacgat cagggctccg gcgaggatgt
      421 tcgagaagac ggggatgccg atcaggtagg ttgccacgac gagcgcgggg agggtcagaa
      481 tgtcgccgaa ggcggtgatc atgggcgccg ctatgttgtc catgtcccat tctcgttcga
      541 cgctgaggcg ggcaaccaca accgtgatgc cgagcaggag gaccccggcg atcatcccgc
      601 cgatggtcga gatcacaacg tagtcgaaga tgcttagttc ggtccttaca ccgaggaact
      661 cgcagagaag gcgcgccgcg aacgccagga agaccccgga gatgagcgag agtatcgccg
      721 ccgcctgcac gttctcggcc aggacgcccc gcctcaggtt gaagctgaag aggccggtct
      781 gtatggctgt ggagagccgg ctgcccatcg ccccgaagat ggctccccgc atcccgatgg
      841 ccgcgggtat gaggacggcg agcccgacca gctcgtccag gatgccggcg atgccggcga
      901 gcgcgatgcc ggccacgagc tcgcccgtag acgagagaaa gagcgccgcg aacccctgcc
      961 gcagcgactc tttctcctcg gtgaggtacc cgtacacttt tggccggata cgcgggcgct
     1021 ccctgatcct gcgccgcacc gccgctcctc ccgcgcgcac gcgagagcct cttttttctg
     1081 tttcggccac tctggatgta tagaccctga ctgaaacctt gtaaagtccg gcgggccggc
     1141 aggttgccat tggacagggc aggggctaca ctcttctcgc tatttccccg ggcgcgagca
     1201 ggaggtgagt ggtacgtgag taatgacggc ttagatccaa gcagtactag cggaccgcct
     1261 gccgcaagcg tccggtaaca cgagaccccg gaacctggag ttccagtcca actttcctga
     1321 ccacctccgc tggcgagcgg tctcgaggcc ctcggcctgt ctacggaaag ggtgaagcga
     1381 ggtgtaggga tgatgatagt agataatgcc atctatgtgg atgggcgccg gacggtcgag
     1441 ccacacacgg ttagagagac ccacgaggct gtgcgcgaca cgcacgggct ggcctggatc
     1501 ggcctctaca agccgaccga ggaggagttc gcagcggtcg ccggcgagtt cggattgcac
     1561 gagcttgcgg tcgaagacgc catcaaggcg caccagcggc ccaagatcga gcgctacggc
     1621 gacacgctct tcgtagtttt gaagccggcc aggtaccttg acgctccgga agaggtcgag
     1681 ttcggcgagg tgcacgtctt cgtgggacgg gacttcgtca taacggtgcg ccacggtgaa
     1741 gctccggccc tggccgaagt tcgcagcagg ctcgagggag aacccgacct cttgcggctg
     1801 gggcccgagg cgatcctcta cgcgatcatg gaccgggtgg tggatggcta tgcgccggtg
     1861 gtagatggcc tggaaaacga catagacgag atagaggtcg aggtcttctc gggcaacccc
     1921 ggcgtgaccc ggcgcatcta cgagctctcg cgcgaggtga tcgaattcca gcgggctacc
     1981 aagccgctaa ccgggatgct ggcacggctg atagccggct tcgagaagta cgatgtggac
     2041 accgagctgc agcgctacct gcgcgacgtg caggaccacg tggtacaggt ggtggagcgc
     2101 gtcgagggct tccgcgagct cttgcagaac atcctgagcg ttaacctcac cctcgttagc
     2161 gtccagcaaa acgaggaggt caagagcctg accggggcga gcatagacca gaacgaagag
     2221 gtaaagaaga tcagcgcctg ggccgcgatc ctgttcgctc caaccctcat aggaaccgtc
     2281 tacggcatga actttgacta catgcc
//
LOCUS       DASTUV010000498         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_694428, whole genome shotgun sequence.
ACCESSION   DASTUV010000498 DASTUV010000000
VERSION     DASTUV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694428"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFT03_10035"
     CDS             complement(<1..943)
                     /locus_tag="VFT03_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU4848527.1"
                     /translation="MAKSIGIDLGTTNSCVAVLEGGDPVVITNAEGERTTPSVVAFDR
                     RSGERLVGQLARRQAVTNPDRTIYSIKRFMGRRFGDVKQEASRVGYEVKSGSGGDIRV
                     QVGDKDYSPPEISAMILQKLKRDAEEYLGEEVSEAVITVPAYFEDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDKEQDQTILVFDLGGGTFDVSILELGDGVFEVKATSGN
                     NHLGGDDFDAKVVEWMVAEFKKAEGIDLSKDKMATQRLVEAAEKAKRELSSTTSTSIN
                     LPFITADQNGPKHLDLTLTRAQFNNLTADLVEATAGPV"
     gene            complement(1141..1701)
                     /locus_tag="VFT03_10040"
     CDS             complement(1141..1701)
                     /locus_tag="VFT03_10040"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU4848528.1"
                     /translation="MTWSHLPALVAVGAALLASYLGFRRSTTTGEVGARLSSSLVPRI
                     LGSLSDDALSRDGTLDLPERFERMGAAEFEAEISFLLRALGYAVRPTPPSSIHDVDLI
                     LEMAGRRVAVQLKRWKAPVGDRSVYGLFAGRIHHATHEAWLITTSRFTSRAVKLASTT
                     GVRLVDGAELAGWLEASGNAEVERGS"
     gene            2232..2924
                     /locus_tag="VFT03_10045"
     CDS             2232..2924
                     /locus_tag="VFT03_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848529.1"
                     /translation="MEETILLVDDDAALLEVTSIVLASEGYRVLTAEDGVEALRILGR
                     ETLDLVVLDIMLPRMSGFEVLKKMREQSDVPVVLLTAKSQSVDKVVGLELGADDYITK
                     PFDTKELLARIKAILRRFGRQEGKDRDGVIRLGALELDPNGYTVSREGGPLDLTPTEF
                     KILALLMRRPGQAFTRAQISEAVSTGSHYLASRYIDVHISRLRAKVERDPSRPEIIQT
                     VPSVGYRAARPA"
     gene            2974..>3488
                     /locus_tag="VFT03_10050"
     CDS             2974..>3488
                     /locus_tag="VFT03_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848530.1"
                     /translation="MPALGFKARIFVALVGISSVTSLIIGLVLYYFAQDRLVEAENTL
                     LEQRSRTANAGAQDFLEGLRNPEDQTLPPANTYADELVQAVANPTGLEVLFVGPEGKP
                     LAARDGFGDPRNPGKAYDALGLGKETFAEVAESSGGAGRLIKGEGSDYVAVWPLADPG
                     GGARGVMVYDVP"
BASE COUNT          609 a         1136 c         1096 g          647 t
ORIGIN      
        1 tgacaggccc ggccgtcgct tcgacgaggt cggcggtcag gttgttgaac tgcgcccgcg
       61 tgagcgtcag gtccaggtgc ttcggaccgt tctggtcggc tgtgatgaac ggcaggttga
      121 tcgaggtgct cgtggtcgaa gagagctctc gcttggcctt ctcggccgcc tcgacgagcc
      181 gctgggtcgc catcttgtcc ttggagaggt ctatgccctc ggccttcttg aactcagcga
      241 ccatccactc gacgaccttg gcgtcgaagt cgtcgcctcc gaggtggttg ttgccactcg
      301 tcgccttgac ctcgaagacc ccgtcgccga gctccaggat ggaaacgtcg aacgtcccgc
      361 cgcccaggtc gaagacgagg atggtctggt cttgctcttt gtccaggcca tacgccagcg
      421 ccgccgccgt cggctcgttg atgatcctct tgacgtccag acccgcgatg cggcccgcgt
      481 ccttggtcgc ctggcgctgc gcgtcctcga agtacgccgg gaccgtgatg accgcctcac
      541 tcacctcctc gccgagatac tcctcagcgt cccgcttgag cttctggagg atcatggccg
      601 agatctccgg cggcgagtag tccttgtccc cgacctggac cctgatgtca ccgccagagc
      661 cggacttgac ctcgtagcct actcgggaag cctcctgctt cacgtcgccg aagcgccggc
      721 ccatgaagcg cttgatcgag tagatcgtgc ggtcagggtt cgtcaccgcc tgcctgcgcg
      781 caagctgccc aaccaaccgc tccccactcc tccgatcaaa cgccaccacc gagggcgtcg
      841 tacgctctcc ctccgcgttc gtgatcacca ccgggtcccc accctccaat accgccacgc
      901 aactgttggt cgtgcccagg tctatcccta tgctcttggc catgactgta cctcccgcaa
      961 ggtctactta tctataagca gaagaagtat aaaatcttag tgcagtgtac gcaagtttgt
     1021 gccaattgca tcacgccgcg tgactaccca cacgttcgcc accccgatct tcggcacgat
     1081 ccggcctacg aacccgagca cccgtccgag caccctgcca ggaccacggc gatggtcatc
     1141 tcaagacccg cgctcgacct ccgcgttacc cgaagcctcg agccagcctg cgagctctgc
     1201 gccgtccacc agccgcacac cggtggtgct ggccagcttg acggccctgg atgtgaagcg
     1261 gctggtggtt atgagccacg cctcgtgggt ggcgtggtgg atcctacccg cgaacagccc
     1321 gtacaccgac ctgtccccca ccggcgcctt ccaccgcttc agctgcaccg ctaccctgcg
     1381 acctgccatc tccaggatga ggtcgacgtc gtggatgctc gaaggtgggg tcggtcgcac
     1441 ggcgtatccc agggcgcgca gcagaaagga tatctcggcc tcgaactccg ccgcccccat
     1501 gcgctcgaac ctctcgggga gatctaacgt cccatcccta ctgagtgcat catcggatag
     1561 actccccaga atcctcggga ccagcgacga cgagagcctc gctccgacct cgccggtagt
     1621 cgtcgaacgc cggaacccaa ggtagcttgc gagcagcgcc gcacccacgg cgacgagggc
     1681 cggcaggtga gaccacgtca tcaccccatg caccacctca ctcttctgcc ccgggcggca
     1741 ttccggggcc gtccgcgcct agctcccctt ccggtcatac cctctccctc gctctaaccg
     1801 tcgacctgaa aagctggttc aggtagacct caccttcgac caccctctac ttcaggcggg
     1861 tagaggtccg ttcgggctgt atcgaggggt cctatcccga tgtctacatc atcgtaccct
     1921 catcgccgag tcgccgggag ccagtctcac gctgtcaatt gcgttacgag cggcgtgtgc
     1981 atactgcata tggtggcctg gcgtatggcg cacactgctg cttgtgggtt gagaaaatcg
     2041 tgagtgcggt gcaatgggcc tcaattcccg ggggtttcgc ggcctcgcac tccggacggg
     2101 gcgtccggca atggctcggg ttggagaaag ctcccgggct ggtgggtgat acttcgcgaa
     2161 caagctgctt ctagggtagg attatgtcac tggaggcggt gaacgcggtt agcctggagg
     2221 ttacaagggg tttggaagaa acgatcctgt tggtggacga cgacgcggcg ctattggagg
     2281 taacctccat cgtgctggcg tcggagggat atcgggtgtt aacggcggag gacggggtgg
     2341 aggcgctccg gatactcgga cgggagacgc tggacctcgt cgtgctcgac atcatgttgc
     2401 cgcggatgag cgggttcgag gtcttgaaga agatgcggga gcagagcgac gtcccggtag
     2461 tgttgctgac ggccaagagc cagtcggtag acaaggtggt cggactcgag ctcggggctg
     2521 atgactacat aaccaagccc ttcgacacca aagagttatt ggcgaggatc aaagccatac
     2581 tacgccggtt cgggcggcag gagggcaagg accgcgacgg ggtcatcagg ctcggggcgc
     2641 tggagctcga cccgaacggc tacaccgtga gccgggaggg gggcccgctg gacctgacgc
     2701 ctacggagtt caagatcctg gcgctgttga tgcgacgtcc ggggcaggca ttcacgcggg
     2761 cccagatctc ggaggcggtc tccacggggt cacactacct ggccagccgc tacatagacg
     2821 tccacatcag caggctcagg gccaaggtcg agcgcgaccc gtcgaggccg gagatcatcc
     2881 agactgtccc gagcgtcggg tacagggctg cccgacccgc ctagtctcct ttggagggaa
     2941 aggatagtta aggggagcga gagaagaagg catttgccgg cgctgggctt caaggcccgc
     3001 atcttcgtcg cgctcgtcgg catctcctct gtgacgagcc tgatcatcgg tctggtcctc
     3061 tactacttcg cccaggaccg gctggtcgag gcggagaaca ctttgctcga gcagcgctcg
     3121 cggacggcga acgcgggcgc gcaggacttc ctcgagggtc tgcgcaaccc cgaggaccag
     3181 accctccctc ccgccaacac ctacgctgat gagctggtgc aggcggtagc caacccgaca
     3241 ggcctcgagg tactcttcgt cgggcctgag ggcaagcccc tcgcggcgcg cgacggcttc
     3301 ggagaccccc ggaacccggg gaaagcatac gacgctctgg gcctcggcaa agagacgttc
     3361 gcggaggtgg ctgaatcgag tgggggtgcg ggccgtctaa taaagggtga gggctccgac
     3421 tacgtggcgg tgtggcctct cgcggaccct ggcggtggcg cgcggggcgt catggtctac
     3481 gacgtccc
//
LOCUS       DASTUV010000499         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1767548, whole genome shotgun sequence.
ACCESSION   DASTUV010000499 DASTUV010000000
VERSION     DASTUV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767548"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..143
                     /locus_tag="VFT03_10055"
                     /pseudo
     CDS             75..143
                     /locus_tag="VFT03_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(352..425)
                     /locus_tag="VFT03_10060"
     tRNA            complement(352..425)
                     /locus_tag="VFT03_10060"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:Ile,seq:gat)
     gene            567..1067
                     /locus_tag="VFT03_10065"
     CDS             567..1067
                     /locus_tag="VFT03_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU4848531.1"
                     /translation="MSADGFTRMSIYGINLDLFSSSPIVILKVEDENRYLPIWIGQPE
                     ARSILMKLQNQEFSRPLTHDLAVNLVSELGGSMEKVTVTELRDSTFFAVISVEIDGRT
                     VEIDSRPSDAIALAVRAGAEIFAADEVIEEAAVVFEEAMEETPEDEVVDKFKDWMNQV
                     SPEDFK"
     gene            complement(1392..1637)
                     /locus_tag="VFT03_10070"
     CDS             complement(1392..1637)
                     /locus_tag="VFT03_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848532.1"
                     /translation="MKARAATPDATSARAAAEASVGIHGGPPVLTSALALALAVALAL
                     GGSLDAAEIVAAAVEPAVAVTATLADALAEPEAEASA"
     gene            1694..2104
                     /locus_tag="VFT03_10075"
     CDS             1694..2104
                     /locus_tag="VFT03_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848533.1"
                     /translation="MRTKVVMVLAVVAALVAGTVSAVMAQSSPPASAPPATVSKQCYS
                     KPCYGNANREVIYERIGDGKSDLIRAFGNFDRLHANTYSRDQDQLYGYGGDDFVYVDD
                     GDTKDAAVGGTGFDWCYVDATIEAANSCDKVVVR"
     gene            complement(2220..>2496)
                     /locus_tag="VFT03_10080"
     CDS             complement(2220..>2496)
                     /locus_tag="VFT03_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848534.1"
                     /translation="GPENVDLLMVRDRDTGRFLYTERLERRPGETPWEYVRRSVRREA
                     RIRERFKEERLQVIVGWGAGSVDEFLESYPEYGPVQKTHGEAGGNAR"
BASE COUNT          472 a          849 c          729 g          446 t
ORIGIN      
        1 ccaacgagat cggcggcatc atcaacccct ccaacctccg cagctccctc agggccctgc
       61 tcaagcgcgc cggcctgccg gatatccgct tccacgacct aaggcataca tgcgccaccc
      121 tgctgctctc tcggaacgtc aatcccaaga tcgtctccga gatgctgggg cactcctcca
      181 tagccatcac cctcgacacc tactccccaa catgcagcag agcgccgtgc gcgccctcga
      241 agaggccctc aagtaacgtt cgcaagcgcg ttgctgcaca aaggccccgg agcatgtccg
      301 gggccttcgc tcgttcctct tttttacctg caaaacgcta cttttttgac gtgggcctgc
      361 ctggactcga accagggacc tcttccttat cagagaagcg ctctaaccgg ctgagctaca
      421 ggcccgaagc ggaggttgcc gccgggataa tttacaacac ggcggggatc ctgacaagcc
      481 gattgaacac cggcgaggcg ggaagtacaa tcggcacggt atcagaaagt cactacgctt
      541 cgcgttgtga gaggagcata tagctagtga gcgccgacgg cttcaccaga atgtccatat
      601 acgggatcaa ccttgacctc ttttcctcca gccctatcgt catcctcaag gtcgaggacg
      661 agaaccgcta cctgccgatc tggatcggcc agcccgaggc gcgttcgatc ctcatgaagc
      721 tccagaacca ggagttcagc cggcccctga cccacgacct ggccgtcaac ctggtctccg
      781 agctcggtgg ctcgatggag aaggtcaccg tgaccgagct tagggactcg accttcttcg
      841 cggtcataag cgtcgagata gacgggcgca ccgtcgagat cgactcccgt ccatccgacg
      901 ccatagccct cgcggtgcgc gccggagcgg agatcttcgc cgcagacgaa gtgatcgagg
      961 aggccgccgt agtcttcgag gaggcgatgg aggagacccc agaggacgag gtcgtggaca
     1021 agttcaagga ctggatgaat caggtctccc ccgaggactt caagtaacgg tagccccacc
     1081 tgcgcctcgc cgctaatact caacatccct gagcggcacc cggtacccgc gcacgccccg
     1141 gcccgtcagt ccccctgtaa ccaacactcg cccgcattcc ggaacgggga tttccaggaa
     1201 tccgcgatca tgcaaagctg gtggacggca cgcgtggatt gtgccgatta tctgacgcct
     1261 gctatgcttg gaatagtgtt gggtgcactc gaggaaaggg aggaatgaca gatgctgtca
     1321 agtaggaagc tttcgttgtg gctggccgcg gtcaccctga cgatagtgct ggtcgcatcc
     1381 cccgccctgg ctcaggcgga tgcctccgct tcgggctccg ccagcgcgtc cgcaagcgtc
     1441 gccgtgaccg cgaccgccgg ctccaccgcc gccgccacga tctctgcggc gtccagcgag
     1501 ccgccgagcg ccagtgcaac tgcaagtgcg agcgccagcg cgctggtcag gacaggcggt
     1561 cccccgtgga tcccgacgct cgcctcggct gcggccctgg cacttgtggc atccggcgtt
     1621 gcggccctgg ccttcacgct gcggcgcggc gcttcttagg cgcctctcca ctactagagg
     1681 aaggaaagaa tagatgcgca cgaaagtagt gatggttctg gctgtggtgg cggcactcgt
     1741 cgcggggacg gtcagcgcgg tgatggcgca gtcctcgcct cccgcgtcag cgcccccagc
     1801 caccgtctcc aagcaatgct actccaagcc ctgctacggc aacgccaata gagaggtcat
     1861 ctacgagagg atcggcgatg gcaagtcgga cctcataaga gccttcggca acttcgaccg
     1921 actccacgcc aatacctact cgagagacca ggaccagctc tacggctacg ggggcgacga
     1981 cttcgtctac gtcgacgacg gcgacaccaa ggatgcggcc gttggcggca caggcttcga
     2041 ctggtgttac gtcgacgcca cgatcgaagc ggccaacagc tgcgataagg tggtcgttcg
     2101 ctaacagcgg cgaggggatc gcggcttttc ctgggaagcc gcgatctcca ctctactcgc
     2161 gcaggcggtc cagcaaaccc ttctcgcgct ccttcgcctg ctcggcgagc cggctctcat
     2221 taccttgcgt tccctccggc ttcgccgtgg gtcttctgca cgggcccgta ctcggggtag
     2281 gactccagga actcgtcgac agagccggct ccccacccca ctatcacctg tagcctctcc
     2341 tccttgaagc gttcccggat ccgggcctcc cggcggacgc tacggcggac gtactcccag
     2401 ggtgtctccc cggggcgccg ctcgagccgc tcggtataga gaaagcgacc ggtgtcccgg
     2461 tcgcgcacca tcagcaagtc cacgttctca ggccct
//
LOCUS       DASTUV010000500         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1016721, whole genome shotgun sequence.
ACCESSION   DASTUV010000500 DASTUV010000000
VERSION     DASTUV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016721"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..371)
                     /locus_tag="VFT03_10085"
     CDS             complement(168..371)
                     /locus_tag="VFT03_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848535.1"
                     /translation="MVRISVQVSSGTARFRVMVQAVSIERALEIARRHNPAAECQVSF
                     PIDPEAFFAKNSTPGVETVEEAA"
     gene            complement(748..2835)
                     /locus_tag="VFT03_10090"
     CDS             complement(748..2835)
                     /locus_tag="VFT03_10090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEU4848536.1"
                     /translation="MPKPVGGAQRYMPGLDGLRALAVLAVIAYHLELGWAPGGLLGVG
                     VFFTLSGYLITDLLLGQREAVGHLRLGDFWLRRARRLLPALFLMLAVVVAWVTLFDRS
                     QLPALRGYVAAAAVYMSNWWNIVIHASYFSRFGPPPPLDHLWSLAVEEQFYLVWPFLL
                     WLGLRYARGRYRLAGITLAAAALSAILMALLYQPGVDPTRVYEGTDTRASGLLIGAAL
                     AMVWPSRHLRADLAVRRRLLLDCVGVAGLAVIALLIWRTSQYSPFLYLGGNVLLSVST
                     VGVVAALAHPASWLGPALGWAPLRWLGVRSYGIYLWHFPIIVLTTPSVQRSVDLPLQI
                     LQVGATIAVAALSWRFVEEPIRRGALGRIWARTRSRVWRRKAVPRQTVPRRIYRRQRR
                     QRIAIGIVSIAALSVLALDGAGLAGVIGSGAEVGSGSQPPYANAPEEIKLSTHHAPAA
                     TPDTSPTKTTKSPEAASTNTDSQKAASHKPEPQKSTPATLTSCEAVVYIGDSTSEGMV
                     MPSYLPDPDLRLSAQLARVGATTQYIEISGARSTVETLSGQANAYDVASQLVANGFRG
                     CWMLALGTNDTANIYAGSTIERMARIERMMSVVGDQPVLWVNVKSLLTSGPYAESNME
                     LWNRTLVQACSRYPKMRVFDWASATKYGWFISDGTHYTSTGYAQRARLTADALVHAFP
                     ATGAEKSPGCVVH"
     gene            complement(3449..3940)
                     /locus_tag="VFT03_10095"
     CDS             complement(3449..3940)
                     /locus_tag="VFT03_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848537.1"
                     /translation="MAEYSSGTATGAAFRLGPLPGEPGEVRASFLLWLTAVAAGVLET
                     IIRIIYSLSVSSWGEADVTGVIIRVIVYTVAVYIIAQMRLGKRWARIALAVLFGGIGT
                     LSLVIDPISWLAAGNSFREVFTQADLLFFIIAPVRAAHLAAVIAALVLMFLPAANDYF
                     RHP"
     gene            complement(4011..>4205)
                     /locus_tag="VFT03_10100"
     CDS             complement(4011..>4205)
                     /locus_tag="VFT03_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848538.1"
                     /translation="LFRRPIDDEEVEFVTIMWFDSWEAVKRFAGEDYERAYVPSKARE
                     VLARFDERSQHYEIRERLDY"
BASE COUNT          819 a         1358 c         1317 g          711 t
ORIGIN      
        1 tccaaccgtc gtctccctct ccgccacgac atgctcctct cccccagcaa cccatcataa
       61 ttccgggggc acgagccccc accggtttca gtctaccaga gcgcggttgc gggccgcccc
      121 atcacggcgc acagaagaaa agagagacgg tagattgtcg aggcggccta cgctgcctct
      181 tcgaccgttt cgaccccggg ggtagagttc ttggcgaaga aagcctcggg gtcgatagga
      241 aaagacacct ggcactccgc ggcggggttg tgccggcgag cgatctcaag cgcccgctcg
      301 atgctcacgg cctgcaccat caccctgaaa cgcgcggtgc cgctgctcac ctgaaccgag
      361 atccgtacca tctccctacc atcctcctgt tgggaacagc gacgcgtgcc tctgtgcgcc
      421 gctcttgctc ctactgccac gcaatgtaga gcatatttgt tacatcccgg taacagacgt
      481 ataaacaatc ggagacaatt ccgggccgga gcgagtacca acgatgcccc cgaggccgcc
      541 gaggcggatc ccgcgatgct aaccggactt gaggtctccg tccactttcc cgacctggga
      601 atcctgggcg caggcggact ctcagctcaa caggtcagga aggttgaggc gctggcagtg
      661 gaggataatc ccaacctggc tttcgagcgg acagaaagta tgccgctctg gaatagccgc
      721 cggatagtgc aaatatggga actcccgcta gtggaccacg cagcccggac tcttctctgc
      781 cccggtggcg gggaacgcgt ggaccagcgc gtcggcggtc agacgcgcgc gctgggcgta
      841 gcctgtcgag gtgtagtggg tcccgtccga gatgaaccag ccgtatttgg ttgccgatgc
      901 ccagtcgaag acccgcatct tcgggtacct ggagcaggcc tgtaccaggg tacggttcca
      961 gagctccatg ttggactccg cgtagggtcc actcgtgagc aaggacttca cgttaaccca
     1021 cagcaccggc tggtcgccga ccaccgacat catgcgctcg atccgggcca tccgctcgat
     1081 ggtcgaacca gcgtagatgt tagcggtgtc gttggtgccc agagcgagca tccagcaccc
     1141 ccggaagccg ttcgccacca gctggctcgc tacatcgtag gcgttggcct ggcccgatag
     1201 cgtctcgacg gtcgacctcg cacccgagat ctcgatgtac tgggtggtgg cgccaacacg
     1261 cgctagctgc gcgctcaggc gaagatcagg gtcgggcaag tagctgggca tgaccatgcc
     1321 ttccgacgtg gagtcgccta tgtatacgac cgcctcacac gaagtgaggg tggctggcgt
     1381 ggacttctgc ggctctggct tatgcgaagc agccttctgc gaatcagtgt tcgtcgacgc
     1441 agcttcgggg gacttcgtcg tctttgtcgg agaggtgtcg ggagtagcag ctggtgcgtg
     1501 gtgtgtggag agcttgatct cctcgggcgc attcgcgtaa ggcggctggg acccgctgcc
     1561 cacctcggcg ccgctgccta tgaccccggc gaggccagct ccgtccagtg cgaggacgct
     1621 gagcgctgct atcgagacta tcccgatagc aatccgctgc cgacgttggc gccgatagat
     1681 ccgtcgtggg acggtctgtc gtgggaccgc tttccggcgc cacacccgtg atctggttcg
     1741 cgcccagatg cgcccgaggg cgccgcggcg tatcggttct tcgacgaatc gccaggacag
     1801 ggcggccacg gcaatggtcg cccccacctg gaggatctgc aggggaaggt ctacgctcct
     1861 ctgcactgat ggcgtcgtga gcacgatgat cgggaagtgc cacaggtata tgccgtagga
     1921 gcgcaccccg agccagcgca gcggggccca ccccagcgcc gggcccagcc agctggcggg
     1981 atgggcaagg gcggccacga ccccgaccgt actcaccgac agcagcacgt tcccgccgag
     2041 gtagagaaac ggcgagtact ggctcgtacg ccagatcagg agcgcaatga cggccagccc
     2101 ggcgaccccc acgcagtcca gaagcaggcg acgccggacg gcgagatccg cgcgaaggtg
     2161 gcggctcggc cacaccatgg ccagggcggc gcctatgagg aggccagaag cgcgggtatc
     2221 ggttccttcg tagactcgcg tgggatcgac ccccggctgg taaagcagcg ccatgaggat
     2281 ggccgagagg gccgcagctg ccagggtaat gccggcaagc cggtagcgcc cacgagcgta
     2341 acgcaggccg agccagagca aaaaaggcca gaccaggtaa aactgctcct caaccgccag
     2401 ggaccacagg tggtcgagcg gtggcggcgg cccgaagcgg gagaagtagg aggcgtggat
     2461 tacgatgttc caccagttgc tcatgtagac ggcggcagcg gctacgtacc cccggagcgc
     2521 cggcagctgg gatcggtcga agagcgtgac ccatgccacc accacggcca gcatcaggaa
     2581 aagggcaggc aggagccgcc gggctcgccg gagccagaaa tcgccgagcc gaaggtggcc
     2641 aacggcttct cgctgcccga ggagcaggtc ggttatcagg taaccactca aggtaaagaa
     2701 cacgcccacg cctagcagac cccccggcgc ccagcccagc tccaggtggt aggcgatgac
     2761 ggcaagcacc gcaagggccc gcagcccgtc gagcccgggc atgtagcgct gggcgcctcc
     2821 gaccggctta ggcacgacgc tcccagacca gcagcgcagc ccagcgagtt cccttcgctc
     2881 ccggcagccg gaacggtatg aaggttccgt agtgcgaacc catgctcacc gcgaccgggt
     2941 ccagcgtccg gttactggcg cagacgcgag agcccccagc ctgtagtagc ctgggcgctt
     3001 acctggagtt aaggacccta ttcccgatga accggtccgc caccgctccg gtctcagggt
     3061 catgatccct ccaggattct agcggggact tcccgccgag catcttcgat ggtgcgaccc
     3121 aactgttttc cctcctgggt cctgacatca gagtaaatct tctcactata tcggggggcg
     3181 tgtctaatgc cctcgagccc atatacggat agtttcgagc cgatctccgt cgaaggggag
     3241 tggtgggggt tcgcgcttcc ggagagggca ccagcatccg cgtgcgaatc ttatcgggca
     3301 cgtaacggtc gctctcgagg gtcgtcgccg tcagtatcag ctagcagagc gaacccggcc
     3361 ggtttcactc tcgaaaaggc acaagcactt acatccgcgt tctctgtttc gccgccgctg
     3421 cggtcggaga tccgggagta gcggccgact aagggtgccg gaagtagtcg tttgcagccg
     3481 gtaggaacat caacaccagc gccgcgatga ccgccgcgag atgagcggcc cggacgggcg
     3541 cgataatgaa gaacaagaga tccgcctgcg taaagacctc acggaacgaa ttcccagcag
     3601 cgagccacga gatcgggtct atcaccaggg aaagagtccc gatccctccg aacagcaccg
     3661 cgagggcgat gcgggcccat ctcttcccga gacgcatctg cgcgattatg tagaccgcaa
     3721 cggtatagac gatcaccctt atgatcacgc cggtcacatc agcctcaccc cagctgctca
     3781 cggacagcga gtagataata cggatgatcg tctcgaggac gccagcggcg acggccgtga
     3841 gccacaacag gaacgacgcc cgcacttccc cgggctctcc cggcagtggg ccgagcctga
     3901 acgccgcgcc cgtagccgtt ccgctcgagt attcagccat atctgtgaat ccctttccca
     3961 gacggtgagc ggcgcttttt cacatcccac ttgagtcttt ccgtcccgtg ttaatagtcg
     4021 aggcgttccc tgatctcgta gtgctgtgaa cgctcgtcga accttgccag aacctctcgc
     4081 gccttcgagg gcacataggc ccgttcgtaa tcctcgccgg cgaagcgctt gaccgcctcc
     4141 caggagtcga accacattat cgtgacgaac tccacttctt cgtcgtcgat gggtctgcgg
     4201 aagag
//
LOCUS       DASTUV010000501         4733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_923965, whole genome shotgun sequence.
ACCESSION   DASTUV010000501 DASTUV010000000
VERSION     DASTUV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923965"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..733)
                     /locus_tag="VFT03_10105"
     CDS             complement(299..733)
                     /locus_tag="VFT03_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU4848539.1"
                     /translation="MFAIIDEQIDVGALISAAQRPDCGAISTFVGTTRIDESGGASVK
                     YLEYEAYRPMADRKLEEIGGEIEERWDVREVSIVHRIGRVGPGEASVAIVVASPRRGE
                     AFEASRYAIERIKQVVPIWKREVWSDGYIWVGSQVGTRVDPP"
     gene            complement(736..981)
                     /locus_tag="VFT03_10110"
     CDS             complement(736..981)
                     /locus_tag="VFT03_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU4848540.1"
                     /translation="MDEIKILLFGAAADRAGTRETVVSAGGITLDELWPLLAERYPGL
                     SPMRDTLAFAVNGEYARGDARVAPGDEVAVLPPVSGG"
     gene            complement(984..1907)
                     /locus_tag="VFT03_10115"
     CDS             complement(984..1907)
                     /locus_tag="VFT03_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU4848541.1"
                     /translation="MESSKTRARVICNPASGGGCDPGAVREALGDLDIEWIDTEGSGD
                     AREAAREWGEGLLIVVGGDGTVNEAVNGLGKAGFPEGVTLAVLPMGTGNDLAATLAIP
                     ARIEDAEKVIRENRVRVLDVARVRSEGVEERFFINVATGGFGAETSSLADEELKGRWG
                     KLAYLRASLERARDFEVRDVRVVLDDVEYTIRAVNVAVGNCRYAGGGWLAAPRANPED
                     GLLDLVVIAQVGLKEVLALAPAALAKSDYLDTEGVFSARAKKIRVETRPGGLEFTADG
                     EVIGDEPVEFEVIPRALKVVAGPDYVPEPAG"
     gene            complement(1987..2529)
                     /locus_tag="VFT03_10120"
     CDS             complement(1987..2529)
                     /locus_tag="VFT03_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848542.1"
                     /translation="MTETRNTRQGWLSVPRRREEERLDPHVYKRGINGLTVEEGLRRA
                     FPRVMDILARSGEEYSEFERELVRQTIRACLISQVSRANLYRIAGILVADRRDEGAHL
                     GRSLALAYVAALREAFLWELAVGEMIQTPVGTGEVRYISEDGENAAIKLDGGSRHEVW
                     LPVEVLSRLSPADDFGLRAS"
     gene            2725..3477
                     /locus_tag="VFT03_10125"
     CDS             2725..3477
                     /locus_tag="VFT03_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEU4848543.1"
                     /translation="MNSEEFLQRLKREVIGHPALRHPFLERFGDGEADEEGVRTFGIQ
                     YYRHVRVSRLYIAALISGCREDEGLQLALAGILFDEYGHLRPEETHPALYRRFLRALG
                     IGEEEWEAPRTLPEIELYIDAHYALCLHPDVRLGLGALGPASEWPVPPIYVRLTEGLK
                     KSAGLPDEALEIFTSHVVMDVEHAHIMMEAVAPYAGGEEGQRRVREGAMRSLDARSVM
                     LDGLYRAVFREPVPLLDARPASPGTGVPLDRG"
     gene            3486..4214
                     /locus_tag="VFT03_10130"
     CDS             3486..4214
                     /locus_tag="VFT03_10130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848544.1"
                     /translation="MTEWYREDLAYIHDVGHADFALESAPGILEILARNGIHDGLVVD
                     LGCGSGLWARELVDAGYRVLGIDISEAMIELSRAKVPEAEFRVGSLFDTDIPPCGAVT
                     AISEVLNYLFDEDAGGLAPLFRRVFEALVPGSVFVFDVLGPGQVPPGATAQIFRVGED
                     WAVLAEREEDAGKGTMERRIVSFRKVGEHYRRTDEVHRVRLYEPSELSSELRRAGFEV
                     RTLGAYGDYALGEGHAAFEARKPA"
     gene            4260..>4733
                     /locus_tag="VFT03_10135"
     CDS             4260..>4733
                     /locus_tag="VFT03_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU4848545.1"
                     /translation="MPELPEVETIKEDLRELVVGSTIERAEVLDPALVEQPSTEEFAR
                     RLGGVRITGVRRRAKHLIVELDSGDALVFQLKIGGQLLLVPPVEEPRTALMLVLHLDG
                     GRRLFLRDQTGFTRARVLDEEELEARLSPLGPEPLEEGFAVEYLRETLGSRRAQIK"
BASE COUNT          808 a         1560 c         1540 g          825 t
ORIGIN      
        1 ggatgccgaa gtccacactc gaagaggtgc gatccttgcc catgcccgat aagtatacct
       61 ttcgagcacg ctccgactta cgcctccgct tgtcagcacg ccctcttcga gggcttgtca
      121 gccaggatgg cgcacttcgt cgctatgcaa caccagacag agctactgtc acaacgccgc
      181 ctctaatacg tgaccgggta tgagcccaac ggcagagaga gtaagctgaa atgctgacaa
      241 gcgaggccgc aaggccgagc gtgctgacaa gcggagcccg aagggcgtag cgtgctgatt
      301 agggcggatc taccctggtg ccgacctggc tgccgaccca gatgtagccg tctgaccaca
      361 cctcgcgctt ccagatgggc actacctgct tgatcctctc gatcgcgtac cggctggcct
      421 cgaaggcctc tccgcgtcgt ggagaggcga caactatcgc cacgctggcc tcgccgggcc
      481 caacccgtcc gatccggtgg acgatggaga cctcccgcac atcccagcgc tcctctatct
      541 cccccccgat ctcctccagc ttcctgtcgg ccatcgggcg gtacgcctcg tactccaagt
      601 acttgaccga cgctccccct gactcatcta tcctagttgt ccctacgaag gtggagatcg
      661 cgccgcagtc cggcctctgg gcggcggaga taagcgcccc gacgtctatc tgctcatcga
      721 ttatggcgaa caatactagc ccccggagac cggcggcaga acggcgacct cgtcgcccgg
      781 ggcaacgcgg gcgtcccccc tggcatactc gccgttgacc gcgaaggcta gagtgtcgcg
      841 catgggggag aggcctgggt atcgttccgc caggagcggc cacagctcgt cgagcgtgat
      901 accccctgcg gagaccaccg tctcgcgggt gccggcgcgg tccgcggccg ccccgaagag
      961 cagtattttt atctcgtcca ccgctatccc gctggctcgg ggacgtagtc aggtccggcc
     1021 acgaccttga gggcgcgtgg tatgacctcg aactcgaccg gctcgtcgcc gatgacctcc
     1081 ccgtctgcgg tgaactcgag cccgccgggc cgggtctcga ctcggatctt cttcgccctc
     1141 gctgagaaga cgccctcggt atcgaggtag tcggacttcg ccagcgccgc gggtgccagg
     1201 gcgagcacct ccttgagccc gacctgcgcg atgactacga gatccagtag cccgtcctcg
     1261 gggttggccc tcggggccgc gagccagcct ccgcccgcgt aacggcagtt gcccaccgct
     1321 acgttgaccg cgcggatggt gtattccacg tcgtcgagga ccacccgcac gtcgcgtact
     1381 tcgaagtcgc gagctctctc gagcgatgcc cggaggtacg cgagcttgcc ccaccggccc
     1441 ttcagctcct cgtcggccag gctcgaggtc tcggccccga accccccggt cgccacgttg
     1501 atgaagaacc gttcctccac gccctcggac cggaccctgg caacgtcgag gactctgacc
     1561 cggttctccc ttatgacctt ctctgcgtcc tcgatccttg ccgggatggc tagcgtagcg
     1621 gccaggtcgt tgccggtgcc catgggtagg acggcaagag ttacaccttc tgggaaaccg
     1681 gccttgccca gaccgtttac ggcctcgttc acggtgccgt cgccaccgac gacgatgagc
     1741 agaccctcgc cccattccct cgcggcctcg cgcgcgtcgc ccgagccctc cgtgtcgatc
     1801 cactcgatgt cgagatcccc caacgcctcg cgcacggcac cggggtcgca ccctccgccg
     1861 gaggcagggt tgcagatcac acgcgccctc gtcttcgagc tttccatggg actaatagcc
     1921 cttcgtcgcc tccacgccct cggagtttac ccggaggcaa ccgtcaatat agcagcagga
     1981 actcccctag gaggcgcgaa gaccgaaatc gtcggcgggg gagaggcgtg agaggacctc
     2041 gaccggcagc cacacctcgt gccgggaccc gccgtcgagc ttgatggcgg cgttctcgcc
     2101 gtcctcggag atgtagcgga cctcgcccgt gcccacaggg gtctggatca tctccccgac
     2161 cgccagctcc cacaggaacg cctcccgcag ggccgcgacg taggcgagcg ccagcgaacg
     2221 ccccaggtgg gcgccctcgt cacgccggtc agccaccagg atgcccgcta tgcggtagag
     2281 gttcgccctg ctgacctgcg agatcagaca cgcccgaatg gtctgccgta ccagctcccg
     2341 ctcgaactcg gagtactcct cgccactcct cgccaggatg tccatcaccc tcggaaaggc
     2401 ccgcctcaat ccctcctcga ccgtcaaccc gttgatgcca cgcttgtaaa cgtgcgggtc
     2461 cagccgctct tcctcccgcc gccgcgggac gctcaaccaa ccctgtcttg tgtttctggt
     2521 ctctgtcatg ggtacattat acctcactga agctggtgag acaacgacga atcccctgga
     2581 aatagcgtat ttcaggctac acgcaccggc ctacgacatt ttgctgatgg ccgatcgccg
     2641 gggtctttac ctgaagccaa caacctgaag tctgatgtct accttgcgtt aaactggaag
     2701 cgaagcgaag ggcaggaggc gcaggtgaac tctgaggagt ttctacagcg attaaagcgt
     2761 gaggtgatcg ggcaccccgc gctgaggcac ccgttcctgg agcggttcgg ggatggcgag
     2821 gctgacgagg aaggtgtgag gacctttggg atccagtact accggcacgt gcgggtgagc
     2881 cggctgtata ttgcggccct gatctccggc tgccgcgagg acgaggggtt gcagcttgcg
     2941 ctggccggca tcctcttcga cgagtacggg cacctgagac ccgaagagac gcacccggcg
     3001 ctgtaccggc gcttcttgag ggcgttgggg ataggggagg aagagtggga ggctccgcgg
     3061 acgctgccgg agatcgagct gtacatagac gcccactacg cgctctgcct tcaccctgac
     3121 gtgcggctgg gccttggggc gctggggcct gcgagcgagt ggcccgtgcc accgatctac
     3181 gtccggctca ccgagggctt gaagaaatct gccggattgc cggacgaggc gctcgagatc
     3241 ttcacgagcc acgtagtgat ggacgtcgag cacgctcaca tcatgatgga ggccgtcgcc
     3301 ccttatgcag ggggcgaaga aggccagagg agggtgcggg aaggggcgat gcgctcgctc
     3361 gacgcccgct cggtgatgct cgacggcctc tacagagctg tcttcaggga gccggtcccg
     3421 ctgctcgacg ctcgacctgc gtcgccgggg accggggtgc cactagatag aggctgaacc
     3481 cggccatgac ggagtggtac agggaggacc tcgcctacat ccacgacgtc ggccatgccg
     3541 acttcgcgct ggagtcagcg cctgggatac tcgagatcct ggcccggaac gggatacacg
     3601 atggcctggt cgtggatctg ggttgcggca gcgggttgtg ggcgcgggag ctcgtggacg
     3661 ccggttaccg cgtcctcggc atcgacatct cggaggccat gatcgagctc tcgagagcga
     3721 aggtgcccga ggccgagttc cgggtgggat ccctgttcga caccgacatc ccgccgtgcg
     3781 gcgccgtcac ggcgatcagc gaagtcctca actacctttt cgacgaggat gccggcggac
     3841 tggcgccact cttccggcgc gtcttcgagg cgctggtccc cggcagcgtc ttcgtcttcg
     3901 atgtactcgg tcccgggcag gtgccgcccg gcgccacggc gcagatcttc cgcgttgggg
     3961 aggattgggc ggtgttagcc gagagggagg aggacgcggg gaaagggacg atggagcgcc
     4021 ggatcgtcag cttcaggaag gtcggcgagc actacaggcg gacagacgag gtccaccgcg
     4081 tgcggttgta cgagccgtcg gagctttcct ccgagcttcg gcgggcaggc ttcgaggtac
     4141 gaacgctggg cgcctacggc gattatgccc tgggagaggg ccacgcggcg ttcgaggcgc
     4201 gaaagccggc gtagactgcc ggtgggaacg agggtcttcg gacacggtat aaatacatca
     4261 tgcccgagct gcccgaggta gagaccatca aggaggacct gcgcgagctc gtcgtgggct
     4321 cgaccataga gcgcgcagag gtgctcgacc ccgcgctcgt cgagcagcct tcgaccgagg
     4381 agttcgcccg ccggctcgga ggtgttcgga taacgggtgt gcgaaggcgg gccaagcacc
     4441 tcatcgtcga gctggattcc ggggacgcgc tggtcttcca gctcaagatc gggggccagt
     4501 tgttgctcgt cccgcctgtg gaggagccgc ggacggcgct catgctcgtc ctccatctgg
     4561 acggcggccg gaggctattc ttacgggacc agacgggctt caccagggcg agggtgctcg
     4621 atgaggagga gctcgaggcg cggctctcgc ccctggggcc cgagccgctg gaggaggggt
     4681 tcgctgtaga atatctgcgt gagacgctcg ggagcaggag ggcccagata aag
//
LOCUS       DASTUV010000502         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1645671, whole genome shotgun sequence.
ACCESSION   DASTUV010000502 DASTUV010000000
VERSION     DASTUV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645671"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFT03_10140"
     CDS             complement(<1..310)
                     /locus_tag="VFT03_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU4848546.1"
                     /translation="MSMMRELYTQILMDHYQRPRNRGELENHDLKEHLLNPLCGDEVT
                     VYTRFEGDKVADVKFTGRGCSISQASASMMAQRLVGKNREDVHKEVARFNAMMVGEEE
                     F"
     gene            complement(307..1536)
                     /locus_tag="VFT03_10145"
     CDS             complement(307..1536)
                     /locus_tag="VFT03_10145"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4848547.1"
                     /translation="MYDIREVRRDFPILDRRVGEMKLVYLDNAATSQKPRQVLDTLNG
                     YYEEHNANIHRGVHRLAEEATALYEGSRERVARFLGAPDVRGLIFTRGTTESINLVAH
                     AWGRKNLREGDEILLTEAEHHSNLVPWQLAARATGARLRFIPVREDGTLDMEVASRLI
                     GPRTKMVGCIHSSNVLATVNPVERLAELAHSVEALMLVDGAQSAPHMPVDVASLGCDF
                     FACSGHKMLGPTGVGVLWGRPEILDAMDPFLGGGEMIREVHLEHSTWNELPHKFEAGT
                     MNIAQAVGLGAAVDYLDALGMENVREHERRLGEYAYRRLSEIGDITVYGPGKDRTGLV
                     SFLLPDVHPHDLSQLLDEEGVAIRSGHHCAQPLMRRLGVAATARASFYLYNTEEEVDA
                     LLTALEGARRFLGAFAR"
BASE COUNT          402 a          804 c          683 g          455 t
ORIGIN      
        1 ggaactcctc ctcgccgacc atcatggcat tgaacctcgc gacctccttg tgcacatctt
       61 cgcggttctt gcccacaagc cgctgggcca tcatcgaggc cgaggcctgg gagatggagc
      121 agccccgccc ggtgaacttc acgtcggcga ccttgtcccc ctcgaagcga gtgtagacgg
      181 tcacctcgtc gccgcagagg gggttgagga ggtgctcctt taggtcatgg ttctccagtt
      241 cgccccggtt gcgggggcgt tggtaatggt ccatgagaat ctgcgtgtag agctcgcgca
      301 tcatgctcat cttgcgaacg ctccgaggaa ccggcgggca ccttcgagcg ccgtaagcag
      361 agcgtccacc tcttcctcgg tgttgtaaag gtagaagctc gcccgagccg tcgccgcgac
      421 gccgaggcgg cgcatcagag gctgggcgca gtgatggccg ctccttatgg ctacgccctc
      481 ctcgtccagg agctgggaaa ggtcgtgcgg atgcacgtcc ggtagcaaga aggagacgag
      541 gcccgtccgg tccttcccgg ggccgtagac ggtgatgtcc ccgatctcgg agagcctccg
      601 gtaggcgtac tctcccaacc tccgctcgtg ctcccgaacg ttctccatcc ccagagcgtc
      661 gaggtagtcc acggccgccc cgagccccac ggcctgggct atgttcatgg tcccggcctc
      721 gaacttgtgg ggcagctcgt tccaggtaga gtgctcgaga tggacctccc ggatcatctc
      781 ccccccgcca aggaacgggt ccatggcatc caggatctcc ggccggcccc acagcacccc
      841 gacccccgtg gggccgagca tcttgtgccc gctgcacgcg aagaagtcgc agcccaaaga
      901 agctacgtcc accggcatgt ggggcgcact ctgcgccccg tccaccaaca tcagcgcttc
      961 gacgctatgc gccagctccg ccagcctctc gaccgggttg acggtggcga gcacgttcga
     1021 ggagtggatg cagccgacca ttttggtcct aggaccgatc agacggctcg ccacctccat
     1081 gtctagcgtg ccgtcctccc ggacggggat gaagcgcagc ctcgcgcctg tcgcgcgcgc
     1141 cgctagctgc cagggtacga ggttcgagtg gtgctcggcc tcggtgagca agatctcgtc
     1201 gccttcgcgc aggttcttgc ggccccaggc gtgggcgaca aggtttatgg attcggtcgt
     1261 gccgcgggtg aagatcaggc ccctgacgtc cggcgcgccc agaaaccgag ccaccctctc
     1321 gcgagaccct tcatagaggg ccgtggcctc ctcggccagg cggtggacac cgcggtggat
     1381 gttggcgttg tgctcctcgt agtacccgtt gagggtgtcc agcacctgcc gcggtttctg
     1441 ggaggtcgcg gcgttgtcga ggtagacgag cttcatttcg cccaccctac gatccaggat
     1501 gggaaagtct ctgcgcactt cccttatgtc atacacttct ctttacctcc ggcgatctcg
     1561 aacaggccgc ggcttcgggc ccccgaccga gcgagtcctc gagtactccc gcgccgggtc
     1621 cactcgcgtc caacagtagt ttccatcccg gccagggcga aatcggagag ctgtcccgta
     1681 gcctccggga tcctctcaaa cactaccaga catcatccca cttcgatgcc ccgaagcgac
     1741 cgccgttgtg aggaggagca cgagccacac ccctaccaga acggcggcga gcggtttcct
     1801 taacggacga ttttcctcaa accaccatcc cttcctccta tacctggtgg cccgcggagg
     1861 ttgcaaacgc ttcgtgtaac cctctccaaa agccgcgggt atcatcctag ggccggatcg
     1921 ctcactaatt cggcccttct ttatgccctt atccacagaa gtgagtagaa gactgctacg
     1981 agcctcggtc ggcccgcaac tccactatct gacggtccac gtcctctaaa gcctcgccga
     2041 gcctggcctc caggcgcgcc ctccggtgat aggcgaggtt tcggctcaac cctctagctt
     2101 cttctaggag cacgcgcatg ttctgcagtt ccgaaagctg cgattctgcc atctgcctga
     2161 catctgagcc tcgttctcgt cctgtagaca tggaagcatt ctactgcttg tacagcactt
     2221 ctcctctatt cacccgagtg cgtggaaaga tgaattctcg gaagttcgct gtggcaagcg
     2281 gaccgccccc ctacccgcac cctggcgcca ggccggtccg agaaaccccc cgaacgcacg
     2341 cgcc
//
LOCUS       DASTUV010000503         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1767790, whole genome shotgun sequence.
ACCESSION   DASTUV010000503 DASTUV010000000
VERSION     DASTUV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767790"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="VFT03_10150"
     CDS             <1..1973
                     /locus_tag="VFT03_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU4848548.1"
                     /translation="PSVMDEIPDPEEGEYDFSKDLFPKLLEQDRPLYGFVTDAYWEDI
                     GTLEQYASAQRDVLDGGVRDVRPPGTRLRENIYVGQRAHVDDEELEGPVVIGDNVRID
                     EGAEISPYTVIGNNVVISAGATVERSIIAEGTYIGEGAELIDTLVGRNSYVQARARIL
                     ERSALGDDVIVGEGATISPEVKVYPHKTVQSGANVTQSLIYETMGLRTVFRGGVVSGK
                     FNVDLTPEFVIRLASSFGSTLDPGAVVTLGRDSSLSAQVAKRAMTAALLGTGVNVRDL
                     RAAHAGVVRHDVLAGKSSAGAHARAGDDPDDVEILFFSSDATPMTESEQRDVEKYFVR
                     EEYRRAHGDDIGELIYPGRAVEQFVERVERAVDREKTSGATIVVDFYSGVAGLVASRV
                     FSRLGMNAVVMEGFANANAVGGAVRKELQEGIERVARVVPTVGSAFGAVVGPEAEHVQ
                     FVDDKGEFVPPDVMLACLIDRLRPRKVVLPINLSRQYARLVEDNGGTVEWSRTGLDNV
                     AIKAAASRADLAGLADGRYIFPTFLPAPDCFITLARALEAFRDEPLSKTRAKFGERFG
                     NVVRERLECPWSAKGRVMRGLAERFGGDPDAILTDGVKLNVDGGWVLMLPDPDNPVFY
                     VYAEPDKANGTRPRELMKDYVELVRSLIEDED"
     gene            2077..2574
                     /locus_tag="VFT03_10155"
     CDS             2077..2574
                     /locus_tag="VFT03_10155"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU4848549.1"
                     /translation="MGSSRGHSRVVGGGGAGVTVCANCGAGITPEMKFCPECGTRLDH
                     GQSTASYAPAFEEAEAPPAGELEGAALIELDQVEGTAGRRMHDIKEATVTVGRGPESD
                     IFLDDVTVSRKHAEIERGERGYRIRDVGSLNGTYVNRVRVDAVDLRNGDEIQVGKYRF
                     KFVFA"
     gene            2595..>3120
                     /locus_tag="VFT03_10160"
     CDS             2595..>3120
                     /locus_tag="VFT03_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4848550.1"
                     /translation="MRERERPVENGGTKDSYTIGEVVARLRPEFPTLSVSKIRYLERR
                     RLINLSRTRGGYRLFSNQDIELLRYILTLQDKEYLPLKVIKKRIEGGAPVTSTDSAGD
                     LSRVLEDRTYTRDELAETLETETRFVDDLMAVGVIGRSGELTQRDAEIARMALEMAKY
                     AIQPRHLRGIMAAVE"
BASE COUNT          599 a          994 c         1027 g          500 t
ORIGIN      
        1 agccttctgt catggacgag atacccgatc ctgaggaagg cgagtacgac ttctccaagg
       61 atctctttcc caagctgcta gagcaggaca gacccctcta cggcttcgtc accgacgcct
      121 actgggagga catcggcact ctcgagcagt acgcctcggc ccagcgcgac gtgctcgacg
      181 gcggggtgcg cgacgtcagg cccccgggga cacgtctcag ggagaacatc tatgtcggcc
      241 agcgcgcgca cgtcgacgat gaggagctcg agggccccgt cgtcatcggc gacaacgtgc
      301 gcatcgacga gggcgccgag ataagcccgt acaccgtgat cggcaacaac gtcgtcatat
      361 ccgccggggc gaccgtagag cggagcatca tcgccgaggg cacgtacatc ggcgagggcg
      421 cagagctcat agacaccctc gtcgggcgca actcctacgt tcaggctcgc gcgcgcatcc
      481 tggagcgcag cgctctaggc gacgacgtca tagtcgggga gggggccacg atctcccccg
      541 aggtcaaggt ctacccccac aagacggtac agagcggggc gaacgttacc cagtccctga
      601 tctacgagac catgggactg cgcaccgtgt tcaggggcgg tgtcgtatct gggaagttca
      661 acgtggacct gacccccgag ttcgtgatac gcctcgcctc ttccttcggc agcacgctcg
      721 atcccggagc agtcgtaacc cttggccgcg attcctcgct ctccgcccag gtcgccaaga
      781 gggcgatgac ggccgccttg ctcggcacgg gcgtgaacgt tagagacctc cgcgcggctc
      841 acgccggggt tgtcaggcac gacgtgctcg ccggcaagtc ctcggccggc gcccacgccc
      901 gcgcgggaga cgaccccgac gacgtggaga tcctcttctt ctcctccgac gccacgccca
      961 tgacagagtc cgagcaacgc gacgtcgaga agtacttcgt gcgcgaggag taccgcaggg
     1021 cgcacggcga cgacataggg gagttgatct accccggccg cgccgtcgag cagttcgtgg
     1081 agcgtgtgga acgcgccgtg gaccgcgaga agacctccgg cgccactatc gtcgtggact
     1141 tctacagcgg tgtcgcgggc ctcgtggcga gccgcgtgtt ctcccggctt ggcatgaacg
     1201 ccgtcgtcat ggagggtttc gccaacgcca acgccgtcgg tggcgccgtg aggaaggagc
     1261 tgcaggaggg catagagcgg gtcgctcgcg tcgtgcctac tgtagggagc gccttcgggg
     1321 ccgtggtcgg tcccgaagct gagcacgtgc agttcgtaga tgacaagggc gagttcgtcc
     1381 cgccggacgt gatgctcgcc tgcctcatcg accgcctgcg cccccgcaag gttgtgctcc
     1441 cgataaacct ctcgcgccag tacgccaggc tcgtcgagga caacggcggc acggtagagt
     1501 ggagccgcac gggcctggac aacgtcgcca taaaggctgc cgcctcacgg gccgacctcg
     1561 ccggtctggc cgacgggcgc tacatcttcc cgaccttcct gcccgccccc gactgcttca
     1621 taacgctcgc ccgcgcgctc gaggcgttcc gggacgagcc tctctcgaag acgcgggcaa
     1681 agttcggcga gcgctttggc aacgtcgtcc gcgagaggct ggagtgcccg tggtcagcca
     1741 aggggcgcgt gatgcgcggc ctcgcagaga ggttcggcgg cgaccccgac gctatcctca
     1801 ccgacggcgt caagctcaat gtcgatggcg gctgggtact catgctcccc gacccggaca
     1861 accccgtatt ctacgtctac gccgagcccg acaaagccaa cggaacgcgc cccagagagc
     1921 tcatgaaaga ctacgtcgag ctcgtccgtt cgctcataga agatgaggac tagtctggta
     1981 ctaaagtccc gccatctaag tatgtacttc aggttcaggg ggagttatac ttcggcttat
     2041 gggtgttaac gtcgattcgg ggggagaggc gttcgattgg gtagcagcag gggacattcg
     2101 cgcgtcgttg gaggaggggg ggcgggcgtg acggtctgcg ccaactgcgg cgccgggatc
     2161 accccggaga tgaaattctg ccccgagtgt ggtacgaggt tggatcacgg gcagtccacg
     2221 gcctcctacg cgccggcttt cgaggaggct gaggcgcctc ccgccggtga actcgaaggg
     2281 gcagcactca tcgagctgga ccaggtcgaa ggcacggctg gcaggcggat gcacgacata
     2341 aaagaggcaa ccgtaaccgt cgggcgcggc cctgagagcg acatcttcct cgacgacgtt
     2401 actgtatcga ggaagcacgc cgagatcgaa cgcggcgagc gcggctacag gatacgggat
     2461 gttggcagcc tcaacggtac ctacgttaac cgggttcggg tagacgccgt cgacctgcga
     2521 aacggggacg agatacaggt cggcaagtac cggttcaagt tcgttttcgc ctgagagatt
     2581 gtagataacg taatatgcga gagagggagc ggccagtgga gaacggcggt accaaggaca
     2641 gctacaccat cggcgaggtt gtcgcgcgcc tcaggccgga gttcccgacg ctctcggtca
     2701 gcaagatccg ctacctagag cggcggcgcc tgatcaacct gagccgcacc cgcggcgggt
     2761 acagactttt ctccaaccag gacatagagc tcctgcgcta catcctcacc ctgcaggaca
     2821 aggaatacct cccactgaag gtcataaaga agcgcatcga gggcggagcg ccggtgacga
     2881 gcacggactc agccggcgac ctctccaggg tcctcgaaga taggacctac acccgcgacg
     2941 agctggccga aactctggag accgagacgc gcttcgtgga cgacctgatg gccgtgggcg
     3001 tgatcgggcg cagcggcgag ctcacccagc gcgacgccga gatagcccgc atggccctcg
     3061 agatggcgaa gtacgcgatc caacctaggc acctgcgcgg catcatggcc gccgtcgagc
//
LOCUS       DASTUV010000504         9181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_530176, whole genome shotgun sequence.
ACCESSION   DASTUV010000504 DASTUV010000000
VERSION     DASTUV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530176"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..820)
                     /locus_tag="VFT03_10165"
     CDS             complement(134..820)
                     /locus_tag="VFT03_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU4848551.1"
                     /translation="MGASGVGVLDKSVVILSFLSEGGPATLAEVVGGTGLSRPTAHRL
                     LSALEAHRLVGRSGGRYSLGARLLGWGSRAMDLDLIEAARPVLAALRDETGESTQLYV
                     REGDLRVCVAAVERTGGGLKDIVPVGAVLPLDRGSGGRVLLAWAEDGEGLASSGTAEV
                     RRRGWAESVAEREAGVASVSAPVFGTGGALRAAVCASGPVSRLGEHPGERLAGPVVAA
                     AREIEGALGS"
     gene            912..1703
                     /locus_tag="VFT03_10170"
     CDS             912..1703
                     /locus_tag="VFT03_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU4848552.1"
                     /translation="MPLEWDASEYEAVSGPQTGWGADFIGVFLERRGLRGDEAAIDVG
                     CGTGRVTELLLRNLPNGTVLAVDASKDMVEAARRRFAGDPRVRVEWCDVLRLEVEEPV
                     DVIFSTATFHWIMDHERLFGRLAKLLKPGGRLVAQCGGTGNIARTLAVAERVVTEEGF
                     RDAFAGWEEGWNFVGPEVTKARLEAAGFEDVETWLHEEHTQFASVSELARFLRTVVLK
                     HHLSFLPIGNWNSFAHAVAERLAEEWSLVVDYVRLNMLATRSGSA"
     gene            1700..2863
                     /locus_tag="VFT03_10175"
     CDS             1700..2863
                     /locus_tag="VFT03_10175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4848553.1"
                     /translation="MRSAEAVVIGAGVIGASVAYHLAARGCGPVLVLERDLAPGQGST
                     RRATGGFRAQFGSEINVRLSLLSREKLVRFEEELGVDPGYRRCGYLFLASHEAQLDAL
                     RGARSVQRAAGLEDAREVRPEEVEDINPAVRAEGLIGGVFGPTDGFLKPMEVLRGYVE
                     GAQRLGVRFEYGVECTGFELDGPGRIAAVRTPDGDVAAGSVVNAAGPWAASLGRHAGL
                     EVPVEPLRRRVAVTGPFDELPEDMPMTVFLEDGFHLRVRDGRVLLLLPDEPDSSDPFD
                     QSVEDAWVESVWGTARERVPCLEEASIDRAECWAGLYEMSPDGHALLGRVEEIDNLYL
                     ANGSSGHGVMHAPALGQLLAEIMLDGAASTLDTHALRPSRFAEGESNLAFTLL"
     gene            2925..3623
                     /locus_tag="VFT03_10180"
     CDS             2925..3623
                     /locus_tag="VFT03_10180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848554.1"
                     /translation="MRLSIEALFDDAAAGYDRTRRQLVPGLDGFYGAALESVPFGRKA
                     EIRVLDLGAGTGLLSSMVAERFPRSRVTLVDLSVEMLRVARRRLSVGNGRFEFRNMDY
                     ARKPLPEGYDLVVSALSIHHLTHGDKKELFWKVFGSLSGGGYFVNADQVHGETPEEEA
                     VYREWWLRRVREAGVTEGDLGAALARMRADRNATLQAQLEWLAKTGFEAVRCAYTDHR
                     FAVYCGRKGKDRDG"
     gene            3616..5019
                     /gene="leuC"
                     /locus_tag="VFT03_10185"
     CDS             3616..5019
                     /gene="leuC"
                     /locus_tag="VFT03_10185"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU4848555.1"
                     /translation="MDRPRTLVEKVWERHVVRRAEGEPDLLYVDLHLVHEVTSPQAFE
                     GLRLAGRQVRRPDLTLATMDHNVPTTDVGLPVKDRVSAKQMEALRNNCEEFGIQLHPW
                     GALGQGIVHIIGPEMGLTQPGLVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     PQSRPKTMAVTVEGELPADVTAKDLMLGILNRIGTSGGVGHVIEYRGEAVRSLSMEGR
                     MTVCNMSIEGGARAGLVAPDETTFEYLEGREYAPKGEEWERAVEYWRTLPTDEGAAFD
                     KEVVIRAEDLVPYVSWGTTPAQTVPLDGEVPEPKNDGHVRALRYMDLKPGTPIREIEV
                     DTVFLGSCTNARIEDLRAAARVLEGKKVKEGIRALVVPGSMRVKKQAEEEGLDEVFTS
                     AGFEWRNAGCSMCLGMNPDILQPGERCASTSNRNFEGRQGKGGRTHLVSPVVAAATAV
                     MGRFASPSELGVPVEVS"
     gene            5020..5607
                     /gene="leuD"
                     /locus_tag="VFT03_10190"
     CDS             5020..5607
                     /gene="leuD"
                     /locus_tag="VFT03_10190"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU4848556.1"
                     /translation="MDPVKKVEGKALPLGWDDVDTDQIVPSDALKRIERTGFGQFLFS
                     QWREDPDFVMNRAEHEDAVVLIAGQNFGSGSSREHAPWAIQDYGFGAVIAPSFADIFK
                     NNCAKIGLLAVELPEEAVQKLLDTVREDPEAGISVDLEGRVVKGPGFEEPFAFDNFAR
                     YRLLNGLDDIGLTLTHEDELEEFEKSRPGYLPKVL"
     gene            5840..7138
                     /locus_tag="VFT03_10195"
     CDS             5840..7138
                     /locus_tag="VFT03_10195"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848557.1"
                     /translation="MPAALRPLRHRDFRLLWTGLAVSLIGSGLWLVALAWQVIELGGG
                     PTELSLVTALYSVGLLAVVLVGGIAADRLPQRLVMLAADLVRAAILLVLGWLSLTGDL
                     RIWHLAAGGLVVGAGEAFFIPSYTAILPHLLPESELLAANGLEGTLRPLAQQATGPVL
                     GGVAVAALSPGVAILTGGLTYLVSAGCLLAMNVRPAKGARTATGLASVFADLREGFGY
                     VRRTSWLWATLLFALALVLFIVGPLEVLLPFAIRDNLGGGADEYGLALAAFGVGGAAG
                     ALAISSRRLPRRYLTVMILMWGLGSLPFAVVGVAEAFWVMFAALFVVGATYSAAMVIW
                     GTLLQRRVPDGLRGRVSSLDFFVSLALMPVSMAIAGPAGAAFGLAAVFLVAGFVPVLL
                     AAAAILVPRLDRDELAHPLDHPVQDHPAMGDSSASGPSAR"
     gene            complement(7192..8115)
                     /locus_tag="VFT03_10200"
     CDS             complement(7192..8115)
                     /locus_tag="VFT03_10200"
                     /inference="COORDINATES: protein motif:HMM:NF040422.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HEU4848558.1"
                     /translation="MTSTVRKITLKRTPCYGPCPVYTVTVFGTGEVRYFGEAHVDKPG
                     AHSWKISRRRLQRLVEAFEKANYSRLLDAYTSREYTDAPGCLTSIEYEDGSSKSVDHY
                     HGDPAAPDALTELEDEIDRILGVERYTEPDLPPEKEHAPAYLLTFNPEKAYKWEDLRD
                     CIEDVRDHGFYATSWSCGRNRKITAGDRVFMMRQGHGSGERRGIFASGWATSEVYQQE
                     HWDQEEARKGKLALYVPIRLDVLLDSDSEQILPRSILKDDPLAGGPWDARTGGVEIPG
                     EIAAELEKEWARLLASKGIAQLHAHRVRTRP"
     gene            8240..9175
                     /locus_tag="VFT03_10205"
     CDS             8240..9175
                     /locus_tag="VFT03_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU4848559.1"
                     /translation="MPEVAIMIEGQDGLDWTRWQRLARTVEDSGYSALHRSDHFTNPT
                     GPVLHALELWSSLTWLADNTDRIEFGPLVSPVSFRHPVITAWTASSVDNLAGGRLRLG
                     IGAGWQEREHEAHGFELLETELRFARFEEALEVITLLLRNDEPVSFDGEFYGLRDAVL
                     MPRSPRPGGPPIVIGGNGPRRTLPLAARYADEWNAVLIPPERFAELSARLDELLREAG
                     RPPEAVRRTLMTRAIFGRTTADVERKLAGKQADNLRARGTVVGTGPEVAEQLGRLDEA
                     GVERVMLQWLETDDIDGLEAMAESVLPRRGRGDRI"
BASE COUNT         1455 a         3007 c         3160 g         1559 t
ORIGIN      
        1 tcatggcgcc cgttctcctg ttgcggcaga taatccgcag catatccaca cactagtagt
       61 ctcgctgcgc cgtacccggg gcgaaaaaga aggagctcct tcgtatcctg gactcggatg
      121 ccgtggcgga tggttacgag cccagagcgc cctctatctc ccgcgccgcc gccacgactg
      181 gccccgccaa tcgctcgccg gggtgctccc cgaggcgtga gacggggccg ctcgcgcaca
      241 ccgccgccct caaagcccca ccggtgccga agaccggcgc gctcacgctc gccacccccg
      301 cctcgcgctc ggcgacactc tccgcccatc cccgcctcct aacctccgcc gtacccgagc
      361 tagctaaacc ctccccatcc tcggcccacg caaggaggac tcttcctccc gagccgcggt
      421 ccagcggcag caccgcgccg accggaacga tgtctttcaa gcccccgccc gtccgctcga
      481 cggcggccac acacacccgg agatctccct ccctcacgta gagctgcgtg ctctccccag
      541 tctcgtccct gagagccgcg agcacaggcc gggccgcctc gatgagatcc aggtccatag
      601 ccctgctccc ccatcccagg agccgcgcgc caagcgagta cctcccccca ctccgcccta
      661 ccagacggtg cgcctccagc gccgataata gccggtgcgc cgtcggacgc gataacccag
      721 tcccacctac cacctccgcc agcgtcgccg gaccaccctc cgagaggaag gagaggatca
      781 ccaccgactt gtctaatacc ccaaccccac tcgctcccat gctcttgctc tccgcgggag
      841 acacttctat actgtccata tagtagacat tctgtctcat atagtgagac aaagcaagaa
      901 gggtggggag cttgccgctc gaatgggacg catccgagta cgaggcggtc tctggaccgc
      961 agactggctg gggcgctgac tttatagggg tcttcctgga gcgccggggt ttgagggggg
     1021 atgaggcggc catcgacgtg gggtgtggga cgggcagggt aacggagctt cttctacgga
     1081 atttgccgaa cgggacggtg ctggccgtgg acgcttcgaa ggacatggtc gaggcggcga
     1141 ggaggcgttt cgcgggtgac ccgcgggtac gggtcgagtg gtgcgacgtt ctgcggcttg
     1201 aagtagaaga gccggtggat gtgatcttct ctaccgcaac cttccactgg atcatggacc
     1261 acgagcgtct cttcggccgt ctcgcgaagc tcttgaagcc ggggggcagg ctggtggccc
     1321 agtgtggcgg caccggcaac atcgcccgga cgctggccgt cgcggagcgg gtcgtgaccg
     1381 aggaggggtt cagggacgcc ttcgccggct gggaagaggg ctggaacttc gtcggtcccg
     1441 aggtgacgaa ggcccgcctg gaggccgccg ggttcgagga tgtcgagacc tggctgcacg
     1501 aggagcacac gcagttcgct tcggtgagcg agcttgcccg cttccttaga acggtcgtgt
     1561 tgaaacacca cctgtcgttc ctgccgattg ggaattggaa ctccttcgct cacgcggtgg
     1621 ccgagcggct ggcggaagaa tggtccctcg tcgtggatta cgtcaggttg aacatgctag
     1681 cgacccggtc ggggagcgcg tgaggtcggc cgaggccgtc gtcatcggcg cgggcgttat
     1741 cggagccagc gtcgcctacc accttgcggc gaggggatgc ggcccggtac tcgtgctgga
     1801 gcgagatctg gctccgggac agggcagcac gagaagagcg accggagggt tccgcgccca
     1861 gttcggctcc gagataaacg tgcggctctc gctactctcg cgcgagaagc tcgtgcggtt
     1921 cgaggaggaa ctcggcgtgg accccggcta ccgccggtgc gggtacctct tcctcgcgag
     1981 ccacgaggcg caactcgacg cgctgcgagg ggcgcgaagc gtccagaggg cggccgggct
     2041 cgaggacgcg agggaggtga ggcctgagga ggtagaggac atcaaccccg cggtccgcgc
     2101 cgaggggctc atcggtggcg tgttcggacc tacggacggc tttctcaagc cgatggaggt
     2161 cctgcgcggc tacgtcgagg gcgcacagcg gctcggcgtg cggttcgagt acggcgtgga
     2221 gtgcacgggt ttcgagctgg atgggccggg gcgtatagcc gcggtgcgca cgcctgacgg
     2281 cgacgtcgcc gcggggtccg tcgtaaacgc cgcgggaccc tgggcggctt ccctggggcg
     2341 ccacgcgggg ctagaggtgc ccgtcgagcc gctgcgcagg cgggtggcgg tgacggggcc
     2401 gttcgacgag ctgcccgagg acatgccgat gaccgtcttc ctggaagacg ggttccacct
     2461 gcgcgtccgc gacggacgcg tcctgctttt gctgccggac gagcccgact cctccgaccc
     2521 cttcgaccag agcgtcgagg acgcgtgggt cgaatctgtc tgggggacgg cgcgggagcg
     2581 cgtgccgtgc ctggaggaag cctccatcga ccgggcggag tgctgggccg gcctctacga
     2641 gatgtcccct gacggccacg cgctgctcgg gagggtcgag gagatagaca atctgtacct
     2701 ggcgaatgga tcttcgggac acggggtgat gcacgccccg gcgctcggcc agctgctcgc
     2761 cgagatcatg ctggacggcg cggcgagcac gctcgacacg cacgctctgc gcccttcccg
     2821 cttcgccgag ggcgagtcca acctcgcctt tacgctgcta tgaaatacac tgaccgttca
     2881 gaaggaggcg gcatggaaga cccgaagacg ttcgtagaga gggtgtgagg ttgagtatcg
     2941 aggctctgtt cgatgatgcg gcggccggct acgaccggac acgaaggcag ctggtccccg
     3001 ggctggacgg cttctacggg gccgcgctgg agagcgtgcc tttcgggcgg aaagcggaga
     3061 tccgggtcct cgacctgggg gcagggacgg ggctcctgag ctcgatggta gccgagaggt
     3121 tcccgcgctc gcgggtaacg ctcgtcgacc tctcggtgga gatgctcagg gtggcgcggc
     3181 ggcgactgtc ggtgggtaac gggcgcttcg agttccgcaa catggactac gcgcgcaagc
     3241 cgttgcctga aggctacgac ctcgtcgtct ctgcgctctc gatccaccac ctcacgcacg
     3301 gcgacaagaa ggagcttttc tggaaggtat tcggctcact ctccggtggc ggctacttcg
     3361 tcaacgcgga ccaggtacac ggcgagacgc ccgaagagga ggcggtctac cgcgagtggt
     3421 ggcttcggcg cgtgcgcgag gcgggggtga cggagggcga tctcggcgcg gccctcgccc
     3481 gcatgagggc cgacaggaac gccaccctgc aggcgcagct ggagtggctc gccaagaccg
     3541 gcttcgaggc ggtccgctgc gcgtacacgg accacaggtt cgcggtctac tgtggccgca
     3601 aaggaaagga tagagatgga tagacccagg acgctcgtag agaaggtgtg ggagcggcac
     3661 gtggtgcgca gggccgaggg ggagccggat ctcctctatg tagacctcca cctcgtgcac
     3721 gaggtgacga gccctcaggc tttcgaaggg ctccggctcg cgggacgcca ggtgcggcgc
     3781 ccggacctca cgctcgcgac catggaccac aacgtcccga cgaccgacgt ggggctgccg
     3841 gtaaaggaca gggtctccgc gaagcagatg gaggctctca ggaacaactg cgaggagttc
     3901 ggcattcagc tccacccgtg gggggctctg gggcagggca tagttcacat catcgggccg
     3961 gagatgggcc tgacgcagcc cgggctcgtg atcgtctgcg gcgactccca caccgcgacc
     4021 cacggcgctt tcggcgcgct cgcgttcggc atcggcacca gcgaggtcga gcacgtcctc
     4081 gcgacccaga cgctgcccca gagcaggccc aagacgatgg ccgtgacggt cgagggcgag
     4141 ctgcccgccg acgtcaccgc caaggatctc atgcttggca tcctgaaccg catcgggacc
     4201 agcggcgggg tcggccacgt catcgagtac aggggcgagg cggtgcgctc cctctcgatg
     4261 gagggccgca tgacggtctg caacatgtcc atcgagggcg gagcgcgcgc cggcctcgtc
     4321 gcgcccgacg agaccacctt cgagtacctg gaaggcaggg agtacgcccc gaagggtgaa
     4381 gagtgggaaa gggccgtcga gtactggcgc acgctcccaa cggacgaggg cgcggccttc
     4441 gacaaggagg tcgtcatacg ggcagaggac ctcgtccctt acgtctcgtg ggggacgacg
     4501 ccggcgcaga ccgtcccgct cgacggggag gtgcccgagc ccaagaacga cggtcatgtg
     4561 cgagccttga ggtatatgga cctgaagcct ggaaccccga tcagggagat cgaggtcgac
     4621 accgtcttcc tcggctcatg caccaacgcc cgtatcgagg acttgagagc ggccgcccgc
     4681 gtcctcgaag gaaagaaggt caaggagggc atccgcgcgc tcgtcgtccc cggctccatg
     4741 cgcgtcaaga agcaggccga ggaggagggg ttggacgagg tcttcaccag cgccggcttc
     4801 gagtggcgca acgccggctg ctcgatgtgc ctcggcatga acccggatat cctgcagccg
     4861 ggcgagcgct gcgcctcgac atccaaccgc aacttcgaag gacgccaggg caagggtggt
     4921 agaacccacc tggtgagccc ggtcgtagcg gcggcgacag cggtgatggg ccgcttcgcc
     4981 tcgccctccg agctcggcgt ccccgtggag gtgagctagg tggaccccgt aaagaaggtc
     5041 gaggggaagg cgctgccgct cggctgggac gacgtggaca cggaccagat cgtgcccagc
     5101 gacgccctca agcgcataga gcgtaccggc ttcggccagt tcctgttctc ccagtggagg
     5161 gaggaccctg acttcgtgat gaacagagcc gagcacgagg acgccgtcgt gctgatagcc
     5221 gggcagaact tcggcagcgg ctcgagccgg gagcacgcgc cgtgggcgat acaggactac
     5281 ggcttcggcg ctgtcatcgc cccatccttc gccgatatct tcaagaacaa ctgcgccaag
     5341 atcgggcttc tcgcggtgga gctgcccgaa gaggcggtac agaagctgct cgacacagtt
     5401 cgcgaagacc cggaggccgg gatctccgtg gacctcgaag ggcgcgtcgt gaagggaccc
     5461 ggcttcgaag agcccttcgc cttcgacaac ttcgcccgct accggcttct caacggcctc
     5521 gacgacatcg ggctgacgct gacgcacgag gacgagctgg aagagttcga gaagtcgcgc
     5581 ccgggctacc taccgaaagt gctgtagcct caggatagtc ttcgacggga gccgccactc
     5641 tcggagcgtc tggtctccgg caggccggct tcatgccgcg cgcttccgcg gttccatagc
     5701 ttcacgctga ctgtgcgccg gtgctgcacc cagtggcgcg accggcgatc cggtgacgta
     5761 tttcgaggtt cctggtgcat aatcgagtag aggtgaggtt catgatcgca cccggacacg
     5821 gcggcttatg gtgagctggg tgcccgcggc cctgcgcccg ctgcggcacc gcgacttccg
     5881 cctgctatgg acgggccttg cggtctcgct cataggcagc ggcctgtggc tggtcgcgct
     5941 ggcctggcag gtgatagagc tcggcggcgg tcccacggag ctctcgctcg tcaccgccct
     6001 gtacagcgtc ggcctgctcg cggtcgtgct ggtcggaggg atcgccgccg accggctgcc
     6061 gcagcggctc gtcatgctcg ccgccgatct ggtgcgcgcg gctatcctcc tggtactcgg
     6121 ttggctctcg ctcaccggcg acctacggat atggcacctc gccgcgggcg ggctggtggt
     6181 cggcgccggg gaagcgttct tcatcccttc ctacaccgcg atattgccgc atctccttcc
     6241 cgagagtgag ttgctcgcgg ccaacggcct cgagggaacg ctgagacctc tcgcacagca
     6301 ggcgaccgga ccggtccttg gaggcgtcgc ggtagccgcg ctctcgccgg gcgtcgcgat
     6361 cctcacaggc gggttgacgt acctggtctc cgccggctgc ctgctggcaa tgaacgtccg
     6421 gccggcgaag ggcgcgcgca cagctaccgg tttggcgtct gtcttcgccg acctgaggga
     6481 gggcttcggc tacgtgcgga ggacgagctg gctgtgggcg acgctcctgt tcgcgctggc
     6541 cctcgtgctg ttcatcgtcg ggcccctgga ggtgctcctg cccttcgcga tccgtgacaa
     6601 cctcggcggt ggggccgacg aatacggcct ggcgctcgcc gctttcgggg tcggcggcgc
     6661 ggccggggcg ctcgcgatct cctcccggcg gctgccgcgg cgctacctga ccgtgatgat
     6721 actgatgtgg ggcctgggct ccctcccgtt cgccgtcgtc ggggtcgcgg aggctttctg
     6781 ggtgatgttc gctgccctgt tcgtcgtcgg cgccacctac tccgccgcga tggtgatctg
     6841 gggcaccctc ctccagcgcc gggtgcccga cggcctgcgc ggccgcgtct cgagcctcga
     6901 cttcttcgtg tctctggccc tgatgccggt ctcgatggcg atagccggcc cggccggggc
     6961 cgcgttcggg ctcgccgccg tcttcctggt cgcagggttc gtacccgtgc tcctggcggc
     7021 cgcggcgatc ctggtcccgc ggctcgatcg cgacgaactc gcgcatccgc tcgatcaccc
     7081 ggtccaggac catcctgcaa tgggagactc ctctgcaagc ggtccttctg cacgttagcc
     7141 actccgagcc ttgtgccggg cccgatgccc gagacctgga tcgcgtcctc ctcagggtcg
     7201 cgtgcggacc ctgtgggcat ggagttgggc tatacccttc gaggccagca ggcgtgccca
     7261 ctctttttcg agctcggcgg ctatctcgcc ggggatctcg acgccgccgg tccgcgcgtc
     7321 ccaggggccg cctgccagag ggtcatcctt cagtatggag cgtggaagta tctgctcgga
     7381 gtcggagtcg agcagtacgt ccaggcggat ggggacgtag agggccagct tgcctttcct
     7441 agcctcttcc tggtcccagt gctcctgctg gtagacctct gacgtggccc aaccggaagc
     7501 gaagatgccg cgccgctcgc cgctcccatg cccctggcgc atcatgaaca cgcggtctcc
     7561 ggcggtaatc ttccggttgc ggccacagct ccacgatgtc gcatagaagc cgtggtctct
     7621 gacgtcttct atacaatctc gcaggtcttc ccacttgtaa gccttctcag ggttgaaggt
     7681 gagcaggtaa gccggcgcgt gttccttctc tggcgggagg tccggctccg tgtatctctc
     7741 gacgccgagg atacgatcga tctcgtcttc cagctccgtg agcgcgtccg gggcggccgg
     7801 gtctccatgg taatgatcta cgctcttgga gctcccgtct tcgtactcga tcgaggtcag
     7861 gcagcccgga gcgtccgtat attcgcgact ggtgtatgcg tctagcagac gggagtagtt
     7921 tgccttctcg aaagcctcta ccaggcgttg cagtctcctc cgggatatct tccagctgtg
     7981 cgcgcccggt ttatcgacgt gggcttcccc gaaatacctc acctctcccg tgccgaagac
     8041 cgtgaccgtg tagaccgggc acggaccgta gcagggagta cgtttcaggg tgatttttct
     8101 taccgtcgaa gtcacagatc cagctctccc aggacccgaa attatacggg aacgcctcac
     8161 cgagccaacc ggctttgccc cgtcagcgga ccggcgatac gattactgct cgttgctgcc
     8221 tggagtcagg aggacccgcg tgccggaagt cgcgatcatg atcgagggcc aggacgggct
     8281 cgactggacc cgctggcaac ggctggcgcg gacggtcgag gactccggtt actccgcgct
     8341 ccaccgctcg gaccacttca cgaacccgac aggccccgtc ctgcacgcgc tggagctgtg
     8401 gtcttcgctc acctggctcg cggacaatac cgaccgcata gagttcggac cgctcgtctc
     8461 tccggtctcg ttcaggcacc ccgtcatcac cgcctggacg gcgagcagcg tggacaatct
     8521 cgccggcgga cgcctgcggc tcggcatcgg cgcgggctgg caggagcggg agcacgaggc
     8581 tcacggcttc gagttgctcg agaccgaact ccggttcgct cgcttcgaag aggctctgga
     8641 ggtcataacc ctgctcctgc gcaacgacga gccggtctcg ttcgacggcg agttctacgg
     8701 gctgcgcgac gccgtgctga tgccgaggtc cccgcggcct ggcggaccgc cgatagtcat
     8761 cggcggcaac ggcccgcggc gcaccctgcc gctggctgcc cgctacgccg acgagtggaa
     8821 cgccgtcctc ataccccccg agcggttcgc ggagctgagc gcccgcctcg acgagttgtt
     8881 gcgagaggcc gggcgcccgc ccgaagcggt gcgccggacg ctgatgacgc gtgcaatatt
     8941 cgggcgcact accgctgacg tcgagcgcaa gctcgcggga aagcaggcgg acaatcttcg
     9001 cgcgcggggc accgtcgtcg gcacggggcc cgaggtcgcg gagcagctcg ggcgcttaga
     9061 cgaggccggg gtggagcggg tgatgctgca gtggctggag acggacgata tagacggtct
     9121 ggaggccatg gccgagtcgg tcttgccgcg gcgagggcgc ggcgacagga tctgaaccgg
     9181 a
//
LOCUS       DASTUV010000505         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_444200, whole genome shotgun sequence.
ACCESSION   DASTUV010000505 DASTUV010000000
VERSION     DASTUV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444200"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFT03_10210"
     CDS             <1..1059
                     /locus_tag="VFT03_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU4848560.1"
                     /translation="STMTPEQVEQQEAQIPEEFHLFSRSIIAIDPPDHTRLRKLVQPS
                     FTGRGMEALRGSIQQIADDLLDEAERDAAGRGEAAPERQMDLIKAFAYPFPVTVISDM
                     LGIPREDRETIRGWTENLLRVDRLRGQEVDEETRAGLREFIDYLKDLFDRKRRTPTDD
                     MISRLVHVEEDGDILDEEEMLATVFLMFLAGHVTTVNLIGNGVVALLTHPDELAKFKA
                     NPELVKGVVEETLRYWGPVDFIGRRIAREDVEVGGTVIPTGEQATVSLASANRDPKRF
                     ANPDAFDISRADANRHVAFGKGIHVCLGAPLARVEGQVAFDTLFRRYPDLRLAVPAEE
                     VQWGASFLRGFARLPVLF"
     gene            1368..3461
                     /locus_tag="VFT03_10215"
     CDS             1368..3461
                     /locus_tag="VFT03_10215"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU4848561.1"
                     /translation="MRLIISEKANAAKKIAQILAEGPVKEGKHRSVPYHTFTWKGEES
                     VSVGLKGHVLNPEFPEEYSNWQKVEPSELIDAEILKSVSEKGVANAVKSLAKKADRVV
                     IATDFDREGELIGVEALRLAFEANPRLIDHVERSRYSALTKGEVTRAFDNLVDVSSEL
                     ADAGAARQDIDLIWGATLTRWVSRATKRYGSAFLSVGRVQSPTLVLIAERERERRAFV
                     PEPYWELVGTLRNGGDPFAVHHAHGRFKDEAEARKAHQNLTDTATVTEVKQKSATRPP
                     PTPFNTTGFLTAAASLGIGPSRAARIAEDLYTDGYISYPRTDNTVYPRSLDLREVLGQ
                     LKRVEGVGQYAEKLLASDKLSPTRGKRETTDHPPIYPTGYASRKALRDDQWKIYQLVA
                     RRFLATLSGPAKTLRTTLRFDSGGEPLTTSGTVVTEEGWLGIYFYGRRADEELPALNE
                     GDEVDVLEAEVLSKETQPPGRYGQGRLIKLMEDFGLGTKATRPNIIQNLYDRGYVHDD
                     PLIPTEKGIALARALKAFASEIASHEMTSELETSMDAISEGKTSKENVVDESRDVLRR
                     VYENLIEAEKEFADIVWDGIRGDETLGKCPESGHNLIIRRNRKSGKRFVGCQGYPECR
                     VTYPLPQKGTIIPLHTKCDACGSPEIKVVGGKRPWVTCINMDCPKKQEQRENKEEGGA
                     GLSREGAQEPVKSTT"
     gene            3623..4390
                     /locus_tag="VFT03_10220"
     CDS             3623..4390
                     /locus_tag="VFT03_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4848562.1"
                     /translation="MAPVIGITATLRQDEASVAQRPLGRFVRADLDYVEGVAEAGGLP
                     VVLPPVVGARETEMLLGGLDGLLLSGGSDLDPCYYGEEAVPELGATVPERDGFEMNLV
                     EHALRSGMPIFGICRGMQVLNVALGGTLYQDLPSQMDHSVLLGHRQETPKWQPTHEVE
                     VDAGSQVAGIMGTGELKVNSYHHQAVKDLASNLVSVAHAPDGVVEAVEWRDLSQRWLV
                     GVQWHAEAMRDAAPQHRKLFEAHVSAAESHALRRAAA"
     gene            4365..>4639
                     /locus_tag="VFT03_10225"
     CDS             4365..>4639
                     /locus_tag="VFT03_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848563.1"
                     /translation="MLCAGLRPEGGTKARASRGIFVTIEGPDFSGKSTLVRAIEPLLA
                     GVEPPTYFTREPGGTPAAERIRRLVLDPEVEMDAWTEAYLYAAARAD"
BASE COUNT          923 a         1437 c         1539 g          740 t
ORIGIN      
        1 tcgaccatga ctccagagca ggtagagcag caggaggcgc agataccgga agagtttcac
       61 ctgttctccc ggagcatcat cgctatagac ccgccagatc acacgcgact gcgcaagctc
      121 gtccagccga gcttcaccgg acggggcatg gaagcgttgc gggggagcat ccagcagatc
      181 gcggacgacc tgttggatga ggccgagcgc gatgccgccg ggcgtggcga ggcggcgccc
      241 gaaaggcaaa tggacctgat caaggcgttc gcctacccgt tccctgtaac ggtcatcagc
      301 gacatgctcg gcatcccgcg ggaggaccgt gagacgatcc gggggtggac cgagaacctg
      361 ctccgcgtcg accgcctccg gggccaggag gtggacgagg agacccgggc aggcctacgg
      421 gagttcatcg actacctcaa ggacctgttc gatcgcaagc gccggacccc cacggacgac
      481 atgatcagcc ggctcgtaca cgtcgaggag gacggcgaca tcctcgacga ggaggagatg
      541 cttgcgacgg tcttcctcat gttcctcgcc gggcacgtga ccacggtgaa cctgatcggc
      601 aacggcgtcg tcgccctgct gacccacccg gacgagcttg cgaagttcaa ggcgaatccc
      661 gagttggtca agggggtggt ggaggagacg ctgcgttact ggggaccggt cgacttcatc
      721 ggtcggcgga tcgccagaga ggacgtcgag gtgggcggga cggttatccc gacaggcgag
      781 caggcgacgg tcagcctcgc ctcggccaat agggatccca agcggttcgc caacccggac
      841 gctttcgaca tctcgcgtgc cgacgccaac cggcacgtcg ccttcggcaa gggcatccac
      901 gtctgcctcg gggcgccgct cgcgcgcgtc gaagggcagg tcgccttcga cacgctgttc
      961 cgaaggtatc cagatctgcg gctggcggtt cccgccgagg aggtgcagtg gggcgccagc
     1021 ttcctgcgcg ggttcgcgcg cctgccggtt ctgttctgag cgtgctaatg aggtcacacc
     1081 accgatagtg ccgctcatct gagctggcgt ggcggcagcc tgagtggagt ggaaaacgag
     1141 cccctaccgc tcggagcgga gggagaaacg ccccctctga cgagaccctt ctttggtggc
     1201 tgaaaggctc cgcctcgcct tgtaagctac tccagtacct tgactttgtg gtaggacacc
     1261 acgaagatca cgaccagacg cgaagggaga gctagtggct cacacggaga acaaggactt
     1321 gctactcggc aacgaagtgt gctttgtcgg tgtaaaatca taacaatatg cgattaataa
     1381 tctctgaaaa agcaaacgcg gcgaagaaga tagcccagat tctggctgag ggaccggtca
     1441 aggaaggcaa gcaccgcagc gtgccttacc acaccttcac ctggaagggc gaggagtcgg
     1501 tctcggtggg gctcaagggc cacgtgctca accccgagtt ccccgaggag tactccaact
     1561 ggcagaaagt cgagccttcg gagctaatag acgccgagat cctcaagtcc gtctccgaga
     1621 agggcgtggc gaacgcggtc aagtcgctgg ccaagaaggc tgatcgggtc gtcatcgcca
     1681 cggacttcga ccgggagggc gagctgatcg gggtcgaggc tttgaggctg gccttcgagg
     1741 ccaacccgag gctcatcgac cacgtcgagc gctcacgcta ctccgccctg accaagggcg
     1801 aggtcacccg tgccttcgac aacctcgtag acgtctcgag tgagctcgcg gacgcggggg
     1861 cagcgcggca ggacatagac ctgatctggg gcgccaccct gactcgctgg gtctcgcggg
     1921 cgacgaagcg ctacgggagc gccttcctct ccgtggggcg cgtccagtcg ccgaccctcg
     1981 tcctcatagc cgagcgcgag cgcgagcggc gcgccttcgt ccccgaaccg tactgggagc
     2041 tcgtagggac cttgaggaac ggcggggatc ctttcgccgt acaccacgcg cacgggcgct
     2101 tcaaggacga ggcggaagcc cgaaaggctc atcagaacct cacggacacc gccactgtga
     2161 cggaggtcaa gcagaagtcg gctacccggc cgccgccgac cccgttcaac acgacaggct
     2221 tcctgacggc cgcagcgtcg ctcggcatcg gcccgtcgag ggcagcccgc atcgcagagg
     2281 acctctacac cgacggctac atctcctacc cccggacgga caacaccgtc tacccgcgat
     2341 cgctagacct gcgggaggtc ctcggccagc tcaagcgggt cgagggggtt ggtcaatatg
     2401 ccgagaagct gctggcttcg gacaaactct cgcccacgcg cggcaagcga gagacgacgg
     2461 accacccacc gatctaccct acgggttacg cctccagaaa ggcgctgcgc gacgaccagt
     2521 ggaagatcta ccagctcgtc gcccggcgct tcctcgcgac cctctcgggt cccgcgaaga
     2581 ctctgcgcac gacgctgcga ttcgactcag gcggagagcc gctcacgacg agcgggactg
     2641 tcgtcacgga ggagggctgg ctcgggatct acttctacgg ccgccgcgcc gacgaggaac
     2701 tccccgcgct gaacgagggc gatgaggtag acgtcttgga ggccgaggtc ctcagcaagg
     2761 agacgcagcc tccaggcagg tacggccagg ggcgcctgat caagctcatg gaagacttcg
     2821 ggctcggcac caaggcgacg cgcccgaaca tcatccagaa cctctacgac cgcggctacg
     2881 tccacgacga cccactcatc cccaccgaga aggggatagc cctcgccagg gctcttaagg
     2941 ccttcgcctc ggagatagca agccacgaga tgacatccga gctcgagact agcatggacg
     3001 ccatctcgga gggtaaaacc agcaaggaga acgtcgtgga cgagtcgcgc gacgtactgc
     3061 gccgcgtcta cgagaacctg atcgaggccg agaaagagtt cgcggacatc gtctgggacg
     3121 gtatccgtgg cgacgaaacg ctgggcaagt gccccgagag cggacacaac ctcatcatcc
     3181 ggcgcaaccg caagagcggc aagcggttcg tcggctgcca aggctacccc gagtgcaggg
     3241 taacgtaccc gctccctcag aagggcacga tcatccccct tcacaccaag tgcgacgcct
     3301 gcggctcgcc ggagataaag gtggttgggg gcaagcgacc ctgggtaaca tgtattaaca
     3361 tggactgccc gaagaagcag gaacaacgcg agaacaagga agaggggggt gcgggtctat
     3421 cgagagaggg cgcgcaggaa ccggtgaagt ccaccacata gaggccagac tccatatcga
     3481 agacgagccc cagcagcgcc tcccctccgt ctacgtccgc ctgaccgggt acgccttgat
     3541 gtggctcatt gcagccgtgg tttgctattc tgccgcttta tagttaagat agatgaagaa
     3601 agcgttaaag gatgagtagg atttggcccc ggtaataggc ataacggcaa ccttgagaca
     3661 ggacgaggct tcggtcgcgc agcggccact tgggcggttt gtgcgggcgg acctcgacta
     3721 cgtcgagggt gtggccgagg ctgggggttt acctgtggta ttgccgccgg ttgtcggggc
     3781 tcgggagacc gagatgctac ttggcgggct ggatgggctg ctcttgagtg gtgggagtga
     3841 cctggatccc tgctattacg gcgaggaggc cgttccggag ctcggcgcca ccgtccccga
     3901 gcgcgatggt ttcgagatga acctggtgga gcatgcgttg cggagtggga tgcccatctt
     3961 tggcatctgc cgggggatgc aggttctaaa cgtggctctg ggcgggacgc tgtaccagga
     4021 tctcccctct cagatggacc attccgtgtt gctggggcat cgtcaggaga cgcccaagtg
     4081 gcagccgacg cacgaggtcg aagttgatgc tggatcgcag gtcgcgggga tcatgggcac
     4141 cggcgagctc aaggtcaact cgtaccacca ccaggcggtc aaggatctcg cctctaacct
     4201 cgtctccgta gcccacgctc ctgatggggt ggtcgaggcc gtcgagtgga gagacctttc
     4261 gcagcggtgg ctggtagggg tccagtggca cgccgaggcg atgcgcgacg ccgcccccca
     4321 gcaccgcaaa ctattcgagg cccacgtctc cgccgcagag agccatgctc tgcgccgggc
     4381 tgcggcctga gggcgggacg aaggcgcgag cctcgcgggg catcttcgta accatcgagg
     4441 gcccggattt ctcgggcaag tccacgctgg tgcgcgctat agaaccgctc ctcgccgggg
     4501 tagagcctcc gacgtacttc acgcgggagc cgggtggtac cccggccgcc gaacgaatcc
     4561 ggaggctcgt actcgacccc gaagtcgaga tggacgcctg gacggaggcg tacctttatg
     4621 cggccgcacg ggcggacca
//
LOCUS       DASTUV010000506         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_805162, whole genome shotgun sequence.
ACCESSION   DASTUV010000506 DASTUV010000000
VERSION     DASTUV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805162"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFT03_10230"
     CDS             complement(<1..466)
                     /locus_tag="VFT03_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEU4848564.1"
                     /translation="MGRILRSGGLLYFVILIVLAVVLVNMLSRGNPDVEELNSQQWKQ
                     AVQDKTFVTGLPKTSENYLTILDEDQTVTGQLDTGKGEPKKFEYSYTQMTDVASQLDK
                     ADVPYSVDPQNTGFWLTLLGTLAPILLIVLFFILFMSSMQGGGNRVMSFGKSR"
     gene            complement(544..1086)
                     /gene="hpt"
                     /locus_tag="VFT03_10235"
     CDS             complement(544..1086)
                     /gene="hpt"
                     /locus_tag="VFT03_10235"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU4848565.1"
                     /translation="MMPDVKEVLITSQEIEEKVREIGARITEDYRGENPLLIGVLRGA
                     VVVMSDLMRNIDLQCELDFMDISSYGSGTSSSGVVRILKDLEEDITDRHVLIVEDIID
                     TGLTLSYLRRSLLARGPASLEICALLSKPSRRRTDLDVKYLGFEVPDEFVVGYGLDYA
                     GAYRNLPDICILKPEVFATG"
     gene            complement(1098..2384)
                     /gene="tilS"
                     /locus_tag="VFT03_10240"
     CDS             complement(1098..2384)
                     /gene="tilS"
                     /locus_tag="VFT03_10240"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU4848566.1"
                     /translation="MDLRSPLALVSGGPDSVALLRVVIALGGEPVVLHVDHGLRGEES
                     REDAEFVRELCHRLNVRYEERRIRLSGDSNLQERARDERYRLAEEVAVRLGLRVVATG
                     HTADDVAETVLMNLARGTGLRGLTGIPPVRGRIQRPLVGRTRREILDYLEEIDQPYRT
                     DPTNLSGKYARNRIRLEVLPVLEELYPAAASNIARAASLVRGDLEVLEELASGTMERR
                     GEEVVLPLDRLMALRPAHRRHAVRLAYTTVVPDTAPLPSNLIEAVLELLEGGEGTRTL
                     DLPGGVVASGRSGEELAFYRGPRSMVSGREEIRVGEEMVFGGWRISAREVSGYDPLDA
                     TRPEVAYLDAGNGPYQVRMAREGDIIRPLGLGGTKKVLRALMDRKVPSDLRRMTPVVV
                     DARGEVAWIVLGELDERYKVDERTEEVLRLEVARNA"
     gene            complement(2542..2970)
                     /locus_tag="VFT03_10245"
     CDS             complement(2542..2970)
                     /locus_tag="VFT03_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4848567.1"
                     /translation="MSEGSASSQGSPLKSEMGNTTISSAVVAQIAGIAAQEVENAQMG
                     GGASAAVGGILQSVAGSVTGSTSGGNYSRGVSVEVGEEEAAVDLTMTADYGQSIPQIT
                     EAVRRNVINRVESLTGLRVTEVNIVVSDVQIPEEQPMLEQ"
     gene            complement(3199..3272)
                     /locus_tag="VFT03_10250"
     tRNA            complement(3199..3272)
                     /locus_tag="VFT03_10250"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3236..3238),aa:Met,seq:cat)
     gene            3345..3740
                     /locus_tag="VFT03_10255"
     CDS             3345..3740
                     /locus_tag="VFT03_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848568.1"
                     /translation="MYTVLLIFILLGCLISVIMALRALGAYLRFRRTRAAFQDEVTEE
                     VARLSARTDELEVSLSALEGRAAELPIQISELQQSLTTLQVLTSALGTSLRQVQKVLS
                     YNALKTLSAARIGKILQTASAQKSRQHTG"
BASE COUNT          711 a         1321 c         1109 g          774 t
ORIGIN      
        1 cacggctctt gccgaagctc atgaccctgt ttccgccacc ctgcatggag ctcatgaaca
       61 ggatgaagaa caatacgatg agcaggatcg gcgccagggt gcctagcaga gtgagccaga
      121 agcccgtgtt ctgcgggtcc accgagtaag gtacgtcagc cttgtcgagc tggctcgcca
      181 cgtcggtcat ctgcgtgtag gagtactcga acttcttggg ctctccctta cctgtatcca
      241 gctgacccgt taccgtctgg tcctcgtcga ggatcgtcag atagttctcg ctggttttgg
      301 ggaggccggt gacgaaagtc ttgtcctgga ccgcctgctt ccactgctgg gaattgagtt
      361 cctccacgtc cgggttgccc cggctcagca tgtttaccag gacgacggcc aagactatga
      421 ggatgacgaa gtacagcagc ccgccgcttc tcaagattct gcccaaagcc tcttaccttc
      481 cacgaattgc gtctaggaca tgggaattat actacctgcg caccgaatgg gacgctcttg
      541 ctctcagccg gtcgcgaaga cctcgggctt gaggatgcag atatcgggga ggttgcggta
      601 ggcccccgcg tagtcgagcc cgtatccgac gacgaactcg tctggtacct cgaagccgag
      661 atacttcacg tcgaggtccg tgcgccgcct ggaaggcttg gagagaagag cgcagatctc
      721 gagcgaggcg ggcccgcggg cgagcaggga acgcctcaag taggagagcg tcagccccgt
      781 gtctatgatg tcctcgacga tcaggacgtg acggtctgtg atgtcctctt cgaggtcctt
      841 gaggatgcga acgacgccac tcgatgaggt gccactacca tatgagctta tgtccatgaa
      901 atccaactcg cactgcagat ctatgttgcg catgaggtcg ctcatcacca cgaccgcgcc
      961 gcgcagtaca ccgatcaaga gcggattctc gcccctgtaa tcctcggtga tgcgcgcccc
     1021 gatctcacgc accttctcct cgatctcctg tgaggttatg agtacctctt tcacgtcggg
     1081 catcatactg gacattctca cgcgtttctt gccacctcca acctgagaac ctcctccgtc
     1141 ctctcatcca ccttgtaccg ctcgtcgagc tcacctagca ctatccaggc aacctcaccc
     1201 cgcgcatcca caacgacggg cgtcatgcgc ctgagatcgc tcggtacctt cctgtccatc
     1261 agcgccctca agaccttctt ggtaccaccg aggcccaacg gccggataat atccccctcc
     1321 cgcgccatcc gcacctggta aggcccgttt ccggcatcga ggtaagccac ctcggggcgc
     1381 gtcgcatcca agggatcgta accagagacc tcccgcgccg agatcctcca ccccccgaac
     1441 accatttcct ctccgaccct gatctcctct cgccccgaaa ccatcgaccg aggccctctg
     1501 taaaacgcga gctcctcccc ggagcggccc gaggcaacca ccccacccgg caggtccagc
     1561 gtccgtgtac cctcaccgcc ttccagaagc tcgagcaccg cttctatgag attggaaggt
     1621 agcggcgcag tatccgggac cacggtcgtg tatgccaggc gcacggcgtg gcgccggtgc
     1681 gcgggccgca atgccatgag cctgtcgagc ggcaggacca cctcctctcc cctcctctcc
     1741 atggttccgc tcgcaagttc ttcgaggact tcgaggtccc cgcggacgag ggaggcggcg
     1801 cgcgcgatgt tgctggccgc cgctggatac aactcctcaa ggacgggcag aacctcgaga
     1861 cggatgcgat tgcgggcgta cttgccgctc aggtttgtag ggtccgtacg atagggctgg
     1921 tctatctctt ccaggtaatc caggatctcc ctgcgcgtgc gcccgacgag ggggcgttgg
     1981 atcctacccc gcacgggcgg gatgccggtg agcccgcgca agcccgtgcc ccgcgccagg
     2041 ttcatgagca cggtttcggc cacgtcgtcg gcagtgtgcc ccgtcgcaac gacccgcagt
     2101 cccagccgga cggccacctc ctcggcgagt ctgtacctct catctcgcgc ccgctcctgt
     2161 aggttggaat caccgctgag cctgatccgc cgctcctcat agcgcacgtt caacctatga
     2221 catagttcgc ggacgaactc cgcgtcttcg cgtgactcct cgccgcgcag accgtggtcc
     2281 acgtgcagca ccacgggttc gccaccgagt gcgattacta ctcgtagcag cgccaccgag
     2341 tccgggccgc cggagaccag cgcaagcggg ctacgaaggt ccatgccgta acgctccgca
     2401 gtccgatgca ccttatccgg gaaactcgcg gatcccatgc cgacagttta gagaagtaca
     2461 gaggctagca aaagaaagga ccggagcgtg gtgccccggt cttccatcct gtgggtgttg
     2521 tagtagctac tccgtctgct cctactgttc tagcattggc tgctcttcag ggatctggac
     2581 gtcgctgacc actatgttca cctcggtcac acgcaggcca gtgaggctct ccacgcggtt
     2641 gatcacgttt cggcgcacag cctccgtgat ctggggtatc gactggccgt agtctgcggt
     2701 catggtcagg tctacggcgg cctcttcctc gccgacctcg acgctgacac ccctcgagta
     2761 gttccctccg ctcgtgctgc ctgtgacgct accggccacg ctctgcagga tcccaccaac
     2821 ggccgcggag gcgccgccgc ccatctgcgc gttctctacc tcctgcgccg cgatgcccgc
     2881 tatctgggcg accaccgcgc tgctgatcgt agtgttgccc atctcgctct tgagtgggct
     2941 tccctggctg ctagcgcttc cctcgctcat ggctcttcct ttccgttcat tcgtcagatg
     3001 ggtggcacct gaacgccacg caggtacccc gtacgtctcc cgccgaggcc ggatccatag
     3061 cttccccacg gctttgtaga cggttatcac gctgcctacc ccgcctttca cgatcctaaa
     3121 ccccgcgtgc caggtccagg cttcagggaa gacgtcccag aaaagcaata accccgcagc
     3181 ttcggggcct ccgagaagta gcgggggcag gatttgaacc tgcgaccttc gggttatgag
     3241 cccgacgagc taccagactg ctccaccccg cgtcgtaggc gaaattctaa cactggcccg
     3301 gagtttgtaa aggcggggag tgccgtggta cggttgccat cgatatgtat acggttctac
     3361 tgatcttcat actcctgggg tgcctgatct ctgtaatcat ggccctccgc gcgctcgggg
     3421 cttacctgag gttccgccgc acccgcgcgg ccttccagga tgaggttacg gaggaggtcg
     3481 cccgcctctc cgcacggacc gatgagcttg aggtcagcct ctcggccctc gaaggccgcg
     3541 ccgcggagct tcccatccag atctccgagc tgcagcagag cctgacgacg ctgcaggttc
     3601 tgacgagcgc actgggcacc tccctgcgcc aggtgcagaa ggtactctcc tacaacgcgc
     3661 ttaagacgct cagcgccgcc cgaatcggca agatcctcca gaccgcttcg gcccagaaga
     3721 gcaggcaaca cactgggtag cgacgccggt gtcaaagacg cgccgtagcc gtatactccg
     3781 gaatgtggac gaagagggcg cacagcaggc acagactacc gaggtactga aaccttcggg
     3841 tgaggactgc gagatctgtg gcgcggagat gtacggcctc cattgcaagc tcatgtgtcc
     3901 caactgcggc taccg
//
LOCUS       DASTUV010000507         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_181538, whole genome shotgun sequence.
ACCESSION   DASTUV010000507 DASTUV010000000
VERSION     DASTUV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181538"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..684
                     /locus_tag="VFT03_10260"
     CDS             190..684
                     /locus_tag="VFT03_10260"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEU4848569.1"
                     /translation="MSDNPVFLYAGEYDSVEDAKADLEELKELHREEVVGTYDAAVIT
                     KNEEGKVKIVDKIEKPTQHGGWAGLAVGAALGLIFPPGVLVTGLLGAGAGALIGHLEG
                     GMSRSDLKEVGDSLDRSEAALIVVGEATIERGVEEATRRAKRELKKEVRADAREMDRA
                     IDAM"
     gene            complement(936..1157)
                     /locus_tag="VFT03_10265"
     CDS             complement(936..1157)
                     /locus_tag="VFT03_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848570.1"
                     /translation="MRRSLEIEASERIVLGCGCGERLVLLGRESDWYAEGRDAFECEC
                     GEKLTLANRLEKGPGIADLIRGPAFLDGT"
     gene            1477..3123
                     /gene="ggt"
                     /locus_tag="VFT03_10270"
     CDS             1477..3123
                     /gene="ggt"
                     /locus_tag="VFT03_10270"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU4848571.1"
                     /translation="MFRSEADVAAGSRGARRPDLGGRSTVYGTKGAVACEHPSAALAG
                     IRVLDAGGNAADACVAMAACMAVLAPMATGMGGDAFLLFYEAETGSVLGANGSGRAPR
                     AATIEKLRERGVTEMPERGGLTITVPGAVRLWEDAVASLGTLPLARLLEPAWELAENG
                     YPVSEVFARYWELGEDLLLKNEAASRALLLDGRAPRAGEIFQQPDLASTISAVAEGGA
                     DAFYNGEIARSIARAAQEAGGYLSEEDLAEHESTWVEPISTDYRGVRVYEIPPPGQGV
                     AALEMLNILEGFEMGDLDPSGADRIHLEVEAKKLAYRDLYEEIGDPEFWLHAEIPTGR
                     LISKEYAAKLRERISPDLASERVPEPTLGEDTTYLCAVDAGGNGCSFINSLYMGFGSG
                     VVAEGTGVCLQNRGNSFRHHPDHPNRLEPGKRPMHTIIPGLATSSATGALWATFGVMG
                     GPMQPQGHAQLLSNLIDHDMSSQDAVDHPRHFHGGDILLVEGRVPEREVARLRSKGHD
                     VEVGANYVIPTGGAQLIRVLDSGVRACGSDPRKDGCALAQ"
     gene            3129..3605
                     /locus_tag="VFT03_10275"
     CDS             3129..3605
                     /locus_tag="VFT03_10275"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU4848572.1"
                     /translation="MSVLEGSPARRVAVAGVVGALYVVLSLAVAPFAFGPLQFRLGEV
                     LKPLVIKYPATIPAFAVGVAIVNLFSPLAGALELALMPAVNLLGGALCWLVARRIGGT
                     VGTYVASFLFALIIAAGVATVLHFAAGLPYLPSFASVAVSETVLLFIGNTLLVRRV"
     gene            3697..>4860
                     /locus_tag="VFT03_10280"
     CDS             3697..>4860
                     /locus_tag="VFT03_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4848573.1"
                     /translation="MNEIESLIFLLGAAALLAQLARVLKVPYPILLVLGGLGIGFVPG
                     LPVVQIAPEVIFIVFLPPLLNAAAFFSSPLDLRAHLRPIILLAIGLVLLTTVAVAAVA
                     HFVVGLPWAAAFVLGAILAPTDPVAAEAIFRRLGVPERVGTVVSGESLINDGTALVAF
                     RVALAALGGGAFSIWEAGLHFVLVGGGGIVVGLVLAWVILPLWGRLREPSIMITFSVL
                     IPYAVYILAEEVLHVSGILAVVTYGLYQGWRAPRLFPDASTRIQALSFWGVLIFVLEA
                     LLFILVGQQLPSILGNLGEYSLAQVLLYSVIVYAVVLGARFAWFFTTPYLHPVFNRLL
                     RNRYLRAPWQERLVMSWSGMRGAVSLAAALAVPVAIEGERFDVRDLILFITFAV"
BASE COUNT          785 a         1544 c         1640 g          891 t
ORIGIN      
        1 aacgaaagta gagtgtccag tgcgagagac gcgtgatgca gaagtgcaag ggagaattgg
       61 cgggatcggt tgagcccaat gacagctagt caccagggtg agcccgggaa ggaggacagc
      121 accccaggaa atctccatta cccgagggcg agaggttgca acgagtaaac ctgagaggga
      181 gacgatgacg tgagtgacaa cccagtgttc ctgtacgcgg gcgaatacga ctctgtggag
      241 gacgccaagg ccgacctcga ggagctcaag gagttacacc gcgaggaagt cgttggaacc
      301 tacgacgcag ctgtgatcac caagaacgaa gaaggcaagg tcaagatagt agacaagata
      361 gagaagccaa cccagcacgg cggatgggca gggctggcag tcggcgcggc ccttggcctg
      421 atcttcccgc ctggtgtcct ggtaaccggg ctgctaggag caggagcagg agcgctcatc
      481 ggccacctcg agggggggat gtctcgctcg gacctcaagg aggtaggcga ttcgctagat
      541 aggagcgagg cggccttgat cgtggtcgga gaggcgacca tagagcgggg tgttgaggag
      601 gcgaccaggc gtgcgaagag ggagctgaag aaggaggttc gggcagacgc cagggagatg
      661 gaccgagcga tcgatgcgat gtaacacctg gggttggcgg accgatcgtc aagcaggtca
      721 gctaggctga cccgccgcgc gagttcggga ggatctgaca ccaacgagac ccgggcatcc
      781 ccgggtctct caaagactcg gtcccaggtc ttctctgccg ggctaattgg tcgggctctc
      841 tggaaagtcc tggcgccgta acacctcttt agttgaggct cacggacctg ccgcgagagt
      901 ttggcagcgc cggatttatc ctggagcccg gctcgctatg taccgtcgag aaacgcggga
      961 ccgcggatca ggtcagcgat gccgggtcct ttctctaggc ggttggctag cgttagcttc
     1021 tcgccacact cgcactcgaa ggcgtcgcgt ccctccgcgt accagtcgct ctcgcgcccg
     1081 agcaggacca gccgctcgcc gcaaccgcaa cccaggacga tccgttccga agcctcgatc
     1141 tccagggacc gccgcatgtg ttgttctccc tctcgctggc ccgttgcagc ataactctcg
     1201 cacgccagga ccgcgcgcac aacgtaaacc tcgctgaagt tgatggtggt tatgcttcag
     1261 gcataaaaaa cctgtgttcc ctaaacttcg agttgaagtt catggtcggt ggtacgaaaa
     1321 agaggtgagc gagctcggta cgcggcgtta ttctgcgcgc cttgccgccg tcggtagttg
     1381 cgtccccttc tctcggtgca tcgggcgcga tgcacctgcg ggcagttctg taaactgtgt
     1441 gagggatcat aggcttacga gaggggctgc gacgccatgt ttagaagcga agcggatgtc
     1501 gccgcgggta gcaggggcgc ccgcaggccg gatctcggcg ggcgctccac ggtctacggg
     1561 acgaagggtg cggtggcgtg cgagcatccc agcgcggcgc tggctggcat ccgggtgctc
     1621 gacgccgggg ggaacgccgc cgatgcctgc gttgcgatgg ccgcctgcat ggccgtcctc
     1681 gccccgatgg cgacgggcat gggcggcgac gcctttctcc tcttctacga ggcggagacg
     1741 ggttctgtgc tcggggccaa cggctccggc cgcgcgccca gggcagccac catagagaag
     1801 ctgcgagaga ggggcgtcac cgagatgccc gagcgtggcg ggctcacgat cacggtcccc
     1861 ggcgccgtcc ggctctggga ggacgctgtg gcttctctgg gtacccttcc gctcgctcgg
     1921 ctgctcgagc ctgcctggga gctagccgag aacggctacc ccgtctccga ggtcttcgcc
     1981 cgttactggg agctcgggga ggatcttctg ctcaagaacg aggccgcctc tcgcgctctc
     2041 ctgctggatg gcagggcgcc gagggcgggg gagatcttcc agcagccgga cctggcttcg
     2101 accatctccg ccgtcgccga gggcggggcc gacgcgttct acaacgggga gatagccagg
     2161 agcattgccc gggccgcgca ggaggcgggc gggtacctga gtgaagaaga cctcgccgag
     2221 cacgagtcca cgtgggtgga gcccatctcg acagactacc gcggggtgcg ggtctacgag
     2281 ataccgcccc cgggacaggg cgttgccgcc ctcgagatgc tcaacatcct cgaggggttc
     2341 gagatgggcg atcttgaccc gtctggcgcg gaccgcatcc acctcgaggt agaggcgaag
     2401 aagctggcgt accgcgatct gtacgaggag ataggcgatc cggagttctg gctccacgcg
     2461 gaaatcccaa ccgggcgtct gatctccaag gagtatgcgg cgaagctgcg cgagcgcatc
     2521 tcgccggatc tcgcctcgga gagggtcccg gagccgacgc tcggcgagga cacgacctat
     2581 ctgtgcgcgg tggacgccgg gggcaacggc tgctcgttca taaacagcct ctacatgggg
     2641 ttcggcagcg gcgtggtagc agagggcacc ggggtctgcc tccagaaccg gggcaactcc
     2701 ttcaggcacc accccgacca cccgaaccgc ctcgagccgg gcaagcgccc gatgcacact
     2761 attatccccg gcctcgccac gagctccgct accggcgcac tctgggccac cttcggggtg
     2821 atgggcggcc cgatgcagcc ccagggccac gcgcagttac tgtcgaacct catagaccat
     2881 gacatgagtt cccaggacgc cgtggaccat ccccgccact tccacggggg cgacatcctc
     2941 ctcgtcgagg gccgcgtgcc cgagcgggag gtcgcgaggc tgcggagcaa gggccacgac
     3001 gtcgaggtgg gagcgaacta cgtgatcccc acgggcggcg cacagctcat ccgcgtcctc
     3061 gatagcggcg tgcgcgcgtg cggcagcgac ccacgtaagg acggctgcgc gctggcgcag
     3121 taagagacgt gtccgtactc gaagggagtc cggcccggag ggtcgcggtc gccggggtgg
     3181 tcggggctct ttatgtagtt ttgagcctcg cggtagcacc cttcgccttc gggccgctgc
     3241 agttccggct cggcgaggtc ttgaaacctc tggtgatcaa gtacccggcg accatccccg
     3301 ccttcgcggt tggggtggcg atagttaacc tgttcagccc gctggcaggc gcgctggagc
     3361 tcgccctgat gccagccgtg aacctgctag gcggtgcgct ctgctggctc gtggcgcgca
     3421 ggatcggggg tacggtcggg acatacgtgg caagctttct cttcgcgctc atcatcgccg
     3481 ccggcgtcgc gacggtgttg cacttcgccg ccgggctgcc gtacctgccg tccttcgcct
     3541 cggtcgccgt ctcggagacg gtactgctct tcattggaaa cactcttctc gtgcggcggg
     3601 tctagctgga cgtcgttata gaatgggatc atgagcatcc ccggacccga attgtcgttc
     3661 ctttcgggta gataggctag gggcattggg gcgcggatga acgagatcga gtcgctcatc
     3721 ttcctgctcg gggccgcggc gttgctcgcg cagctggccc gggtgctgaa ggtgccctac
     3781 ccgatcctgc tggtcctcgg aggcctcggc atcggcttcg tgccggggct gcccgtcgtt
     3841 cagatcgccc ccgaggtcat ctttatagtc tttctgcctc cgctcctcaa cgcggcggcc
     3901 ttcttctcct cgccgctcga cctgcgcgcg cacctcaggc cgataatcct gctcgccata
     3961 ggactcgtcc tccttaccac cgttgcggtt gccgccgtcg cccacttcgt cgtcggcctg
     4021 ccgtgggctg cggccttcgt cctcggggcg atcctcgcgc ctaccgatcc ggtggccgcc
     4081 gaggcgatct tccgtcgcct gggagtgccg gagcgcgtgg gtaccgtagt aagcggcgag
     4141 agcctcataa acgacggcac cgcgctcgtg gccttccgcg tggctctggc cgccctgggc
     4201 ggcggcgcgt tctcgatctg ggaggctggc ttgcacttcg ttctcgtcgg cgggggtggg
     4261 atcgtggtag gcctcgtcct cgcctgggtt atcctgcctc tgtgggggcg gctgagggag
     4321 ccctcgatca tgatcacctt ctccgtcctg attccctacg ccgtctacat cctcgccgag
     4381 gaggtcctgc acgtctcggg catcctggcc gtggtcacct acggcctcta ccagggctgg
     4441 agggccccga ggctcttccc ggacgcctcc acgcgcatcc aagcgctctc cttctggggc
     4501 gtcctgatct tcgtgctgga ggcgctgctc ttcatcctgg tcggccagca actcccctcc
     4561 atactcggga acctcgggga gtactctctg gcacaggtcc tcctctactc cgtcatcgtc
     4621 tacgccgtgg tcctgggcgc gcgcttcgca tggttcttca ccacgcccta cctgcaccct
     4681 gtcttcaaca ggctcctgcg caacaggtac ctgcgcgccc cctggcagga gcggctggtt
     4741 atgagctgga gcgggatgcg gggcgcggtg tctctggcgg cggccctcgc cgtgcccgtg
     4801 gccatcgaag gggagcgctt cgatgtacgg gacttgatcc tcttcatcac cttcgccgtg
//
LOCUS       DASTUV010000508         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_660048, whole genome shotgun sequence.
ACCESSION   DASTUV010000508 DASTUV010000000
VERSION     DASTUV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660048"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFT03_10285"
     CDS             complement(<1..768)
                     /locus_tag="VFT03_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4848574.1"
                     /translation="MRTKSVEQSIRDTEEPEFQLRKALGPLDLTVFGIGVIIGTGLFV
                     LTGEAAAGYAGPAVALSYVVGGVACALAALCYAEFASSVPVAGSAYTFSYATFGEFIA
                     WMIGWDLILEFTLGAATVAKGWSGYLVSVLEILGIKLPPGLYAATEGFSHDWLALIVV
                     ALVTTILVVGIKLSSMFNQVITGIKILVTLFIIGFGIWFIKLDNLTPFIPPPKPGGEA
                     YGSALDAYLLPSLLGIDTIYGVTGIFTGAALVFFAFIG"
     gene            complement(889..1815)
                     /locus_tag="VFT03_10290"
     CDS             complement(889..1815)
                     /locus_tag="VFT03_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848575.1"
                     /translation="MSEAARQAPVVSGAQVIAAGFAAATAAFVTSRFGVAGTLLGASV
                     TAMIITGGSAILRSYLENVTGNVRKMPRKYQARRERRKAQRYGEPETLSERPDLRDNF
                     AGRLRAALGWFSHLPPLARRSILVKGLIAAAVAFVIGMGAVWGVERVINNSLSCGFWA
                     NCPEGAKPGIHLNDEGQGANPTISLGRAKTKEGVSGSGAQGLPAFRQNAGAQQDGSVQ
                     QNRSFEQGSPDFEQQPAAPRGGGSSDQQGGGLFQPDRPVKEPAAAPVQPETPNSASPA
                     PEESPPPEESAPPGGAKEPGIEAQDASPAPAQ"
     gene            1969..2229
                     /locus_tag="VFT03_10295"
     CDS             1969..2229
                     /locus_tag="VFT03_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848576.1"
                     /translation="MAESREGKFSELFEVIQDYARREYDYQDKALQVLAGSYTFMFES
                     EDMPDARGVVDEILGQYDYVFTTLERGNLDPLSVDAVVKVAL"
BASE COUNT          419 a          766 c          755 g          404 t
ORIGIN      
        1 gcctatgaag gcgaagaaga ccagcgccgc cccggtaaag atgccggtga cgccgtatat
       61 cgtatctatc ccgagaaggc tcggcaggag gtacgcgtcg agcgcgctac cgtaggcttc
      121 tccccccggt ttcggcggcg gaataaaggg cgtaaggtta tccagcttga taaaccatat
      181 cccgaagccg atgatgaaca gcgtcaccag gatcttgatg cccgtgatca cctggttgaa
      241 catagagctt agctttatcc ctacgaccag aatggtcgtc acgagcgcta ctactatcag
      301 cgccagccag tcgtgggaga agccctccgt cgcagcatag agtccaggcg gcagctttat
      361 tccgaggatc tcgagcacgc tcaccaggta tccggaccag cccttggcga ccgtagcggc
      421 acctaaagtg aactccagga tcaggtccca cccgatcatc caggctatga actccccgaa
      481 cgtcgcatac gagaaggtgt aggcgctgcc ggccaccggt accgacgagg cgaactccgc
      541 gtagcagagc gccgccaggg cgcaggctac gccgccgaca acgtaggaga gggctaccgc
      601 gggcccggcg tacccggcgg ccgcctctcc ggtgaggacg aagagccccg taccgatgat
      661 cacgccgatg ccgaagacgg tgaggtccag gggaccgagc gccttgcgca gttggaattc
      721 cggctcttct gtgtcgcgga tggactgctc gacggatttg gtccgcatta ttcccaagtg
      781 gtcctccctc ccagggtctc ccctgaccca tgaaacgcga tccgagcgca ttgtaacggc
      841 tcgcgcgtct aagcgctgag cgggagccca ggaaggagag ccgcgctgct actgcgcggg
      901 ggccggtgag gcgtcctgag cttcgatccc aggttccttc gccccgcctg gtggcgccga
      961 ctcctccggc ggcggcgact cctcgggcgc cggggaagcc gagttcggcg tctccggctg
     1021 cacgggcgcc gcggcgggct ccttgaccgg ccggtcgggc tggaagaggc ctcctccctg
     1081 ctgatcgctg gaaccgccgc cgcggggtgc ggcgggctgc tgctcgaagt cggggctccc
     1141 ctgctcgaaa gaacggttct gctgcacgct gccgtcctgc tgtgcgccgg cgttctgccg
     1201 gaaggccggc agcccctggg ctccggaccc ggagacgccc tccttcgtct tcgcgcggcc
     1261 gagcgaaatc gtcggatttg cgccctggcc ttcatcgttg aggtgaattc cgggtttcgc
     1321 cccttcaggg cagttggccc agaagccgca cgagaggctg ttgtttatga cccgctcgac
     1381 gccccacacg gcgcccatgc cgatcacgaa cgcgaccgcg gcagctatga gacccttgac
     1441 cagtatcgag cggcgcgcca gcggcggcag gtgcgagaac cagccaagag ccgccctcag
     1501 gcgccccgcg aagttgtccc gcaggtcagg gcgctccgac agcgtctccg gttccccata
     1561 acgctgggcc ttacgccgct ccctgcgcgc ctggtacttt ctcggcatct tgcgcacgtt
     1621 gccggtgacg ttctccaggt agctcctgag gatcgccgac ccgccggtga tgatcatggc
     1681 ggtcaccgag gccccgagca gggtcccggc aaccccgaag cgcgacgtca cgaaggcggc
     1741 tgtcgccgca gcgaaccccg cagcgatcac ctgggctcca ctgaccacgg gcgcttgtct
     1801 ggctgcttcg ctcacgtccc ttaccctacc aaccaacccg ctgggtttca aagagaattt
     1861 cgtcggagtt cgacgggtct ctgtaggcga tttcacaccg cggcgtggtg tggcggcggg
     1921 tggcgcacca cagttagaat tccccggacg gcgacggaaa ggcagaggat ggcggaatca
     1981 agagagggga agttctcgga gctcttcgag gtgatccagg attacgccag gagagagtac
     2041 gactaccagg acaaggccct gcaggtgctc gccggatcct acacgtttat gttcgagagc
     2101 gaggacatgc ccgacgcacg cggcgtagtc gacgagatcc tcggccagta cgactacgta
     2161 ttcaccaccc tggagcgcgg aaacctggac ccattgagcg ttgacgcggt cgtgaaggtc
     2221 gccctctaga atctcttcga gtacggagcc ggcgacgatc ctttcgatgc cccggatgac
     2281 gacctcggcg tccgtgacgt agtcggagta ggtctcgtcg gtgcgcgagc ggcggtagag
     2341 gcct
//
LOCUS       DASTUV010000509         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_337595, whole genome shotgun sequence.
ACCESSION   DASTUV010000509 DASTUV010000000
VERSION     DASTUV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337595"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1023
                     /locus_tag="VFT03_10300"
     CDS             94..1023
                     /locus_tag="VFT03_10300"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEU4848577.1"
                     /translation="MITMRSFAAARTVSALVLFAFAFSACGGEEAGQSSVTAHSKAEA
                     ARAVPSAGGGELAAADPGAPADTGVPADTGVPDDLVAQDFDRKIVKTAELGIRANEVR
                     DAAAKAQQVAAGYGGSVLSSQIEGDGRVSADLVLLIPSPEFEAALDDLRGLGKKVTTD
                     TVRGEDVTEEFVDLQSRERNLLAAEQSLLELYDRANSVNAALAIERELTVVRGEIEQV
                     QGRIKYLENRTASSQISLSIQPVPRPGPPTPAWSAARVVAQAWSASLTVLQALATVIL
                     SALVFGWWLIPVLAAGLLWWKRRARGPSSSEAS"
     gene            1085..1459
                     /locus_tag="VFT03_10305"
     CDS             1085..1459
                     /locus_tag="VFT03_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEU4848578.1"
                     /translation="MMEQESPRVTHLSWGRLEVEGEDGPFKDAKLFPGGARAWDWNET
                     GTSHEPGIQPADVEELLERGATAVVLSKGFHGRLGVTPETLRTLEDRGVAAHVRQTGE
                     AISLYNELRETERVGALIHSTC"
     gene            1705..2331
                     /locus_tag="VFT03_10310"
     CDS             1705..2331
                     /locus_tag="VFT03_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4848579.1"
                     /translation="MSEQYQQQTDSPLVGERGRTTISDSVVSQIAGMAAQEVDGVHMG
                     GSVSRAAGGIIGSITGSEGQTRGVSVEIGTTEVAVDLTMGVEYGRNILEVVEEVRRRI
                     SERVQSLTGLRVTELNATVSDIVFPDDGGGRRAPDGGTSTAEPRTAPDSELTSGEHET
                     TGASPRNQTQTESGPLKEEETSELEPEDVSEARQSSEEDETARRPEDR"
     gene            complement(2593..2850)
                     /locus_tag="VFT03_10315"
     CDS             complement(2593..2850)
                     /locus_tag="VFT03_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU4848580.1"
                     /translation="MGILAWIVVGLIAGLLARLITPGDDPGGIIITILIGIAGAFVGG
                     FVFSIFGGAGMTGFNLWSILVATIGAIILLLIYRLIAGRTT"
     gene            3068..>3259
                     /locus_tag="VFT03_10320"
     CDS             3068..>3259
                     /locus_tag="VFT03_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848581.1"
                     /translation="MAVETVEAAELDDLVIQEFESGLRGRILRPGDADYDVARRHYNA
                     LINKHPALIVQCVGVADVID"
BASE COUNT          641 a         1001 c         1112 g          505 t
ORIGIN      
        1 cgcggatcgc ccgttcatct ttgccatcgc ggagcgggag acgggtgaga tcctgttcct
       61 cggacgcgta acggacccga agaggaggga cctatgataa ccatgagatc ttttgccgcc
      121 gcacggaccg tgagcgcgct cgtgctcttc gcgttcgcct tctcggcgtg cggtggggaa
      181 gaggcgggcc agtcgagcgt gacggcgcac tcgaaagcgg aggcggcccg ggccgtaccc
      241 agcgcgggcg gaggggagct cgcggccgcc gatccgggcg cgcccgctga tacaggcgtg
      301 cccgctgata caggcgtgcc cgacgacctg gttgcgcaag acttcgaccg caagatcgtc
      361 aagacagccg agctcggcat ccgcgcgaac gaggtacgcg acgccgccgc gaaagcgcaa
      421 caggtcgccg cagggtacgg cggcagcgtt cttagctctc agatagaggg cgatggccgc
      481 gtctccgcgg acctcgtgct cctgatccct tccccggagt tcgaggcggc gctcgacgac
      541 ctgcgggggt tgggcaagaa ggtcacgacc gacaccgtga gaggcgagga cgtgaccgag
      601 gagttcgtgg acctgcagtc gcgcgagcgc aatctattgg cggctgagca gagcctgctc
      661 gagctctacg acagggctaa tagcgtcaac gcggcgctcg cgatcgaacg cgagctcacg
      721 gtggtccggg gcgagatcga acaggtgcag ggcaggatca agtacctcga aaaccgcacg
      781 gcctcctccc agatctccct gagcattcag ccggtcccgc gccctggccc gccaacgccc
      841 gcctggagcg ccgcccgcgt cgtggcgcag gcctggagcg catcgctgac cgtcctccag
      901 gcgctggcta cggtcatcct ctctgccctc gtctttggtt ggtggctcat acccgtgctc
      961 gcagcgggct tgctgtggtg gaagcgacgc gcccgcggcc cgagttcctc cgaggcgagc
     1021 tagcggcgag aaccggggcc tccccggtgg taccctgatc ccgctacccg acgtgagagg
     1081 aggcatgatg gagcaggaat ctccgagggt cacccacctc tcctggggac ggctcgaggt
     1141 cgagggcgag gacggcccgt tcaaggacgc caagctcttc ccgggcggcg cgagggcgtg
     1201 ggactggaac gagaccggaa ccagccacga gcctggcatc cagcctgccg acgtcgagga
     1261 gctgctcgag cgaggcgcca ccgcggtggt cctctcgaaa ggcttccacg ggcggctcgg
     1321 cgttaccccg gagacgctgc ggacgctaga agacaggggc gtcgccgcgc acgtccggca
     1381 gacgggggag gctatctcgc tctacaacga gctgagagag accgagaggg taggagccct
     1441 catccactcg acctgctaac cgaagctcac caaggtgagc ttctcagcat ttcggcttac
     1501 tctctgcact gagagaagct gagaagctgc ctggcgaagc caggtggcgt gctgacaagc
     1561 ggaggccgca ggccggagcg tgctgacaag cgaaggcgtc agccggagcg tgctgacaag
     1621 gcgcccgcag ggcgccgtgc tgatagactt ttgggtagat atttggggaa ggctggaacg
     1681 cagatgagcg gaggaggcca aagtatgagc gagcagtacc agcaacagac cgacagcccg
     1741 ctcgtgggcg agcgcggcag gaccaccata agcgattcgg tcgtctccca gatagcgggt
     1801 atggcggctc aggaggtaga cggtgtccac atgggcggca gcgtctcccg ggccgccggc
     1861 ggcataatcg gcagcataac cggctcggag ggccagacgc gcggcgtctc ggtcgagatc
     1921 ggcacgacgg aggtagccgt agacctcacg atgggcgtcg agtatggcag gaacatcctc
     1981 gaggtcgtcg aggaggtgcg gcgcaggatc tccgagcgcg tccagagcct gaccgggctc
     2041 cgggtcacgg agctgaacgc gactgtaagc gacatagtct tccccgacga cgggggaggc
     2101 cgccgcgctc cggacggcgg cacgagtacc gccgagccgc ggaccgcgcc cgatagcgag
     2161 ctaacttccg gcgagcacga gaccaccggg gcgagcccac gcaaccagac gcaaaccgag
     2221 agcggaccgc tcaaggagga agagacctcg gagctagagc cagaggacgt atccgaggcg
     2281 cgacagtcct cagaagagga cgagacggca aggcggcccg aggacaggta gattccaggc
     2341 tcagccgggt ggcgtccagc ctcttgaagc cggacctcga tctcgtctcc tgcggatacg
     2401 acccttggct cgcgtccggg cgtagcagct accactacgc ccacctcggg caaccgctgc
     2461 tggcacgtgc tcttcgacga gctggtcgag cactaccgcg ctctgacccc tcttctggtg
     2521 tagagggcga gccgacacgc aagtagagga cggggcggga agccccgtcc tcctaaaaag
     2581 gtttcctcct gattaagtgg ttcgccctgc tatcaggcgg tagataagca ggagtatgat
     2641 ggctcctatt gtggctacga ggatggacca gaggttgaac cccgtcatcc cggctccgcc
     2701 gaagatgctg aagacgaagc caccaacgaa ggcgccagcg atccctatga ggatcgttat
     2761 tattatgccg ccggggtcgt ccccgggcgt gatcagcctc gccagcaacc cggcgatcag
     2821 tccaactact atccaggcca gtattcccat ctaacgtcct ctctggaggg tttgctcaca
     2881 ataaatctgt acccgcattc cagaaggctc gaaacatcct gtcgtgaatg ttacaaaaat
     2941 ctaggtcggg cgcgggcgga cggcgttggc gaagggggat agacgcccgg gcgtccgttg
     3001 ctgagattca actacctttg tacactatcg tgcaaccata agggtgccgc cagggcgaga
     3061 aaggagcatg gccgtggaga ctgttgaagc ggcggaactg gacgacctgg tcatccagga
     3121 attcgaaagt ggtctgcgtg gcaggatatt gcgcccgggt gatgcggact acgatgtggc
     3181 cagacggcac tacaacgcgc tgatcaacaa acacccggcg ctgatagttc aatgtgtcgg
     3241 ggttgccgac gtcatcgat
//
LOCUS       DASTUV010000510         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1252407, whole genome shotgun sequence.
ACCESSION   DASTUV010000510 DASTUV010000000
VERSION     DASTUV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252407"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFT03_10325"
     CDS             <1..916
                     /locus_tag="VFT03_10325"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEU4848582.1"
                     /translation="GLDLPLQLISFLEEEGSGFGQMLLGSRIIAQRVTEEDLREKFRA
                     TDDGRSFWEHAVEAGYEPERWQDSIRILDDLIGWIELHIEQARVLQDTGNRTGVVNAI
                     AGYVHADVTVHGRGDHAGATPMDFRQDAGVVMAECMLELERLAKEAGGGTVGTVGEIE
                     LRPNLINAVPGEARFSLDIRGVDEDGFRGVARDIAAFAKEAAGKRGMSAEYARRQTLS
                     ATPLDESVVAALEGAAEASGEPYMVMHSGAAHDTMSIADRVPSAMVFVPCKDGISHSP
                     EEDAEPADAALGAEVMLNAIRTFTTSRH"
     gene            977..2404
                     /gene="hydA"
                     /locus_tag="VFT03_10330"
     CDS             977..2404
                     /gene="hydA"
                     /locus_tag="VFT03_10330"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEU4848583.1"
                     /translation="MSVLIKGGRIVTAADDYVGDIFIEDERVTLIGESLDVQADKVID
                     ATGKYVIPGAIDPHTHMEMPFGGTVSCDDFTSGTISAAFGGTTSHVDFCLQEPGQSLP
                     DALATWHEKIERAKPVIDVGFHLAVTDLEEGGTLEDLKKAPDEGVTSYKLFMAYKGAI
                     MVDDETLFRTMQVAAETGALVMVHAENGDAIDVLVKQALAEGKTEPKWHAVTRPPITE
                     GEATNRAIQLARIAGAPLYVVHVSCKEAIDPIAQARTNDWHAWGETCTQYLFIDETAL
                     DQPNFEGAKYVYTPPPRPKENQEHLWHALATDVLSVVSTDHCPFNWDGQKSLGRDDFS
                     KIPNGGPGIENRLHMLHHFGVRQGRISLSRFVELVSTNPAKFFGMYPRKGTIAVGSDA
                     DIVVFDPEKRVKISAQTHRSNVDYNLFEGTEVSGAPEVVLVRGQVIVEGDELVGKPGA
                     GKFVKRARFGEELAGGSGQRAASVR"
     gene            2409..3059
                     /locus_tag="VFT03_10335"
     CDS             2409..3059
                     /locus_tag="VFT03_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU4848584.1"
                     /translation="MSDETREIYERARLGQSVTLGARPAVLVVDFSRGFTDPESTMGS
                     ELTREVEATTRLLAAAREREIPVIFTTIGFEPNLKDGSLWLEKAPGLGELIIGSKWVE
                     IDPRLERRDEETVILKKGASAFFGTNLPSILVSQGVDTIIMCGATTSGCIRATAIDLL
                     QYGYPTLVPRECVGDRAQAPHEANLVDIQAKYADVVSVDDALTYIESVPQKSGASV"
     gene            3101..>3491
                     /locus_tag="VFT03_10340"
     CDS             3101..>3491
                     /locus_tag="VFT03_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4848585.1"
                     /translation="MAKRAPIQEILEDLLRATDASRTTLRLDLPDQDSGLDAVVAEAL
                     APGVRSLRDDTLIRNLRGASTVRFLEEHGRVLVQNDCLADDPAPPSELIEHYGVKAQM
                     LAPIVRVDRLEGLISVHYAPGPRDWSSE"
BASE COUNT          688 a         1111 c         1170 g          522 t
ORIGIN      
        1 cgggctggac cttccactac agctcatctc tttcctggaa gaagaaggct cgggattcgg
       61 ccagatgctt ctggggagcc gcatcatcgc gcagagggtg accgaggaag acttgcgcga
      121 gaagttccgc gccaccgacg acggtcgaag cttctgggag cacgcagtag aggccggcta
      181 cgagccggag cgctggcaag actcgatccg catcctggac gacctgattg gttggatcga
      241 gctccacatc gagcaggcgc gcgtgctcca ggacacgggg aatcggacag gcgtggtcaa
      301 cgccatagcg ggctacgtcc acgccgacgt caccgtccac gggcgcggcg accacgcagg
      361 agcgacgcct atggatttcc gccaggacgc cggcgtcgta atggcagagt gcatgctcga
      421 gctggagcgg ctcgcgaagg aggccggggg cgggacggtc gggaccgtag gggagatcga
      481 gcttcgcccc aacctgataa acgccgtacc gggagaagcc cgcttctcgc tggatatccg
      541 gggcgttgac gaggacggct tccgcggcgt cgcgcgcgac atcgccgcgt tcgcgaaaga
      601 agccgccggg aagcgcggca tgagcgccga gtacgcgcgg cgccagacac tgtcggcgac
      661 gccgctggac gagagcgtgg tggcggcgct ggagggagcg gcggaggcga gcggcgaacc
      721 gtacatggtg atgcactcgg gcgccgcaca cgacaccatg agcatcgcgg accgcgtgcc
      781 gagcgccatg gtcttcgtcc cctgcaagga cggcatcagc cactcgcccg aggaagacgc
      841 ggagccggcg gacgcggccc ttggggccga ggtcatgctc aacgccataa ggacgtttac
      901 caccagccgg cattgaggca taacttaatc aggaaaggaa cgcagtagtg tccgtagaaa
      961 ggggtgcaag cagtttatgt ctgtactgat caagggcggc cgcatcgtca cggccgccga
     1021 cgactacgtc ggggacatat tcatcgagga cgagagggtc accctcatcg gagagtccct
     1081 cgacgtgcag gccgacaagg tcatcgacgc aaccggcaag tacgtcatac cgggcgctat
     1141 cgaccctcac acccacatgg agatgccgtt cggcggcacg gtctcgtgcg acgacttcac
     1201 ctcggggacg atctcggcag ccttcggcgg cacgacgagc cacgtagact tctgtttgca
     1261 ggagccgggc cagagcctcc ccgacgcgct ggcgacgtgg cacgagaaga tcgagcgcgc
     1321 caagccggtg atcgacgtcg gcttccacct tgcggtcaca gacctcgagg agggcgggac
     1381 gctcgaagac ctcaagaagg cgccggacga gggggtgacg agctacaagc tcttcatggc
     1441 ctacaagggc gcgatcatgg tggacgacga gacgctcttc aggaccatgc aggtcgccgc
     1501 cgagaccgga gcgctcgtaa tggttcacgc cgagaacggc gacgcaatag acgtcctcgt
     1561 caagcaggcg ctcgctgagg ggaagaccga gccgaagtgg cacgccgtga cgcgtcctcc
     1621 cattacggag ggcgaagcga cgaaccgggc gatacagcta gcccgcatcg cgggagcccc
     1681 gctctacgtc gtccacgtct cgtgcaagga ggccatagac ccgatagcgc aggcgcgaac
     1741 caacgactgg cacgcctggg gcgagacctg tacccagtac ctcttcatcg acgagacggc
     1801 gctggaccag ccgaacttcg agggagccaa gtacgtctac acgccgccgc cgcgacctaa
     1861 ggagaaccag gagcacctct ggcacgcgct cgccacggac gtcctctccg tcgtctctac
     1921 cgaccactgt cctttcaact gggacggcca gaagtccctg ggcagggacg acttctccaa
     1981 gatccccaac ggcgggccgg ggatcgagaa ccgcctgcac atgctccatc acttcggcgt
     2041 gcggcagggt cgtatctcgc tgagccggtt cgtcgagctc gtctccacca acccggccaa
     2101 gttcttcggc atgtacccca ggaagggcac gatcgcggtc gggagcgacg ccgacatcgt
     2161 cgtcttcgac cccgagaagc gggtgaagat ctccgcccag acgcaccgct cgaacgtgga
     2221 ctacaacctc ttcgagggta cggaagtaag cggcgcccct gaggtcgtgc tcgtccgggg
     2281 acaggtcatc gtcgagggcg acgagctcgt cgggaagccc ggcgccggca agttcgtgaa
     2341 gcgcgcccgc ttcggcgagg agcttgccgg gggatccggg cagcgtgcgg caagcgtgag
     2401 gtgagtcgat gagcgacgag actcgagaga tatacgagcg cgcccgcctc gggcagagcg
     2461 tgactctcgg cgcccgtccg gccgtcctcg ttgtcgattt cagccgtggc ttcacggacc
     2521 ccgagtcgac tatgggctcg gagctcacgc gcgaggtcga ggcgacgacc cgcctcctcg
     2581 ccgccgcccg cgagcgggag atcccggtca tcttcaccac cataggcttc gagcctaatc
     2641 tgaaagacgg ctcgctctgg ctcgagaaag ctcccggact cggagagctg atcatcggca
     2701 gcaagtgggt cgagatagac ccgcgtctcg aacgaagaga cgaagagacg gtaatcctca
     2761 agaagggcgc atcggccttc ttcggcacga acctgccttc gatcctggtc tcgcaagggg
     2821 tggacaccat catcatgtgc ggcgctacga cgagcggctg catcagggcg acagccatag
     2881 acctcctgca atacggctac ccgacactgg tcccccgcga gtgcgttggc gatcgggcgc
     2941 aggcgcccca cgaggcaaac ctggtggata tccaggccaa atacgccgac gtggtgtccg
     3001 tcgatgatgc cctcacctac atcgagagcg tgccccagaa gtccggcgcc tcggtgtagt
     3061 gagaccggcc acggcctcat aaggacaagc gggaggcgtc atggccaagc gggcgccgat
     3121 tcaagagata ctggaggacc tgctcagggc gactgacgcc agccggacga ccttgcgcct
     3181 ggacctgccg gaccaggact ccgggctcga tgcggtcgtg gcggaagccc ttgcgcccgg
     3241 cgtccgctcg ctcagggacg ataccttgat acgcaacctg cgcggcgctt cgacggtcag
     3301 gtttctggag gagcacggaa gggttctcgt gcagaacgat tgcctggcgg acgatccggc
     3361 accaccttcg gagctgatag agcactacgg cgtaaaggcg cagatgctcg cgccgatcgt
     3421 gcgcgtagac cgtctggagg ggctgatctc ggtccactat gctcccggcc cccgcgactg
     3481 gagctctgaa g
//
LOCUS       DASTUV010000511         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_906510, whole genome shotgun sequence.
ACCESSION   DASTUV010000511 DASTUV010000000
VERSION     DASTUV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906510"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFT03_10345"
     CDS             complement(<1..189)
                     /locus_tag="VFT03_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4848586.1"
                     /translation="MADPAENAPLLAAADALARKRLSDKRYGHTVRVSDTAEGLAQIH
                     SLDPNRTRLAALLHDAARE"
     gene            289..1563
                     /locus_tag="VFT03_10350"
     CDS             289..1563
                     /locus_tag="VFT03_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU4848587.1"
                     /translation="MHNGQKNIKSTEIVTIGTEMLLGDLVDTNTAWLSARLATLGVGV
                     YRHTTVGDNRERIVGVLEEAASRADLVITTGGLGPTSDDLTNESLAAVTGREMVEYPE
                     ARAHVDEMFRRFGREPTANNYKQALFPEGTKLIPNPLGTAPGALLEADGTLFATLPGV
                     PSEMKGMFEETLEPLIRDRTEGSIISRTLWFAGIGESALADKVQDFLDASDPTVAPLA
                     GQGKVRLRITTRAATPEEAEERIAPVEKEILSRLGDYYFGEDDETLESAVGRLLIEKG
                     ATLALAESCTGGLLAKRLTDIPGSSAYLEEGLVTYSNESKERLLGVSHELLVEHGAVS
                     EPVARAMAEGAREVAGTNYGLSVTGIAGPDGGTEEKPVGLVFVGLSDADGTYAERLDL
                     SAWARSRDAIRERSANRALDLLRRRLEERDEA"
     gene            complement(1987..>2258)
                     /locus_tag="VFT03_10355"
     CDS             complement(1987..>2258)
                     /locus_tag="VFT03_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEU4848588.1"
                     /translation="RKFGRSLPDNPPSQWRRGMRVKKKANLRPGDLVFFDENRNGKLQ
                     PWDHVGIYSGHRNLIHASSFFGRVVESKMKYIHGYWGARRLKRLS"
BASE COUNT          405 a          694 c          772 g          387 t
ORIGIN      
        1 ctcgcgtgcg gcgtcgtgca ggagggcggc gaggcgcgtc cggttggggt cgaggctgtg
       61 gatctgcgcc aggccctccg ctgtgtcaga caccctcacg gtgtgcccgt agcgtttgtc
      121 ggagagacgc ttgcgggcga gggcgtcggc ggccgcgagg agcggggcgt tttcggcggg
      181 gtctgccacc cggcacattg tagagggaac caccccgact ttgtaggact tccgcatgcg
      241 cgttgtcggg agacgccttc tgagaaggat cgcggtataa ataagagcat gcacaatgga
      301 cagaagaaca taaagagcac cgagatagtc actataggga ccgagatgct gctcggcgac
      361 ctggtggaca cgaacacggc gtggttgagc gctcggctcg ccacgctggg agtgggcgtc
      421 taccgccaca ccactgtcgg ggataacagg gaaaggatcg ttggcgtcct cgaagaggcg
      481 gcctcgcgtg ccgacctcgt gatcacgacc ggcggcctcg ggccgacctc tgacgacctg
      541 accaacgaga gcctggcggc cgtgacgggc agggagatgg tcgagtaccc cgaagcgcgg
      601 gcgcacgtgg acgagatgtt caggaggttc ggccgcgagc cgacggccaa caactacaag
      661 caggcgctct tccccgaagg gacgaagctc atccccaacc cgttggggac cgcgccgggt
      721 gccctcctcg aggccgacgg aaccctcttc gcgacgctcc caggcgtgcc ctcggagatg
      781 aagggcatgt tcgaggagac gctggagccc ctgatccggg atcgcaccga gggctccata
      841 atttcgagga cgctctggtt cgccggaatc ggcgagtcag cgctcgcgga caaggtccag
      901 gacttcctcg acgcttcgga ccccaccgtc gctcctctgg ccggacaggg caaggtgcgc
      961 ctgcgcatca ccacccgcgc ggctacgccg gaggaggccg aggagaggat agcgccggtg
     1021 gagaaagaga tcctctcccg tctcggcgac tactacttcg gcgaggacga cgagactctg
     1081 gagagcgccg tcgggcgcct gctgatagag aagggggcaa ccctcgccct cgccgagagt
     1141 tgcacgggag gtctgctcgc caagcgcctg acggacattc cggggtcctc ggcatacctc
     1201 gaggagggtc tcgtcaccta ctccaacgag tcgaaggaac gcctgctcgg cgtttcgcac
     1261 gaactcctgg tcgagcacgg cgccgtctcc gagccggtcg cccgcgcgat ggccgagggt
     1321 gcgcgcgaag ttgccggcac aaactacggg ctctcggtca ccgggatagc cggtcccgac
     1381 ggcgggacgg aggagaagcc cgtcgggctc gtcttcgttg ggctctcgga cgcggacggc
     1441 acgtacgcgg agaggctcga cctctccgcg tgggcgcgtt cgcgcgacgc catcagggag
     1501 cggagcgcca acagggccct cgatctgctc aggcgccgcc tcgaagagcg agatgaagcg
     1561 taaaaattct cccttgccgt acaccaagca tgatagtaaa cttctcgaag ttgacggcac
     1621 gcgaaagggg gtgatccaga tagacgcatc gagtagtcgc cgcgggtatc ttctgggaga
     1681 ggtggctctg acctagttcc gcccaaatcc ctgcggggtc ctgggtcatt ccttccgcct
     1741 cttcggtagt tgcaggcgga cgtgctgaaa gaccagatac ctgcgtagca ctcagaaggg
     1801 tacggccccc ggacttgtgt ccgggggctc gttctttgcg gcgcgtcacc ttgaccgtgc
     1861 tcggctgacc gaagcagccc cgagatggag gtggcctggc gataggctga agcggagggg
     1921 gtcgcagcca gagtgcgcgg tgatggccgc ggctttttcg caaccgcgcg actggcttgt
     1981 ggggtactag ctcagcctct tgagcctcct cgcgccccag taaccgtgga tatacttcat
     2041 cttgctctcg acgaccctac cgaagaagct ggaggcgtgg atcaggttac ggtgacccga
     2101 gtagattcca acgtggtccc acggctgtag cttgccgttc ctgttctcgt cgaagaagac
     2161 aaggtcgccg ggccggaggt tggccttctt cttcaccctc atgcctcgcc tccactggct
     2221 aggggggtta tcggggagcg atctcccgaa cttcctga
//
LOCUS       DASTUV010000512         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_766656, whole genome shotgun sequence.
ACCESSION   DASTUV010000512 DASTUV010000000
VERSION     DASTUV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766656"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..927)
                     /locus_tag="VFT03_10360"
     CDS             complement(160..927)
                     /locus_tag="VFT03_10360"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU4848589.1"
                     /translation="MPGRLEGKVAVITGGASGIGRETAKRFAEEGARVCVADLADEPG
                     KETAAEIDGLYIHADVTNPDDVQGMYREASERFGGLDVLFNNAGISPPDDDSILETEL
                     EAWQRVQDVNLKSVYLCCKYGIPYLLERGGGSVINTASFVAVMGAAASQISYTASKGG
                     VLAMSRELGVQFARQGVRVNALCPGPVNTPLLQELFAKDPEKAERRLVHLPMGRFAEA
                     TEIANGALFLASDESSYVTASTFLVDGGLSGAYVTPE"
     gene            complement(929..2290)
                     /locus_tag="VFT03_10365"
     CDS             complement(929..2290)
                     /locus_tag="VFT03_10365"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4848590.1"
                     /translation="MLEVLNPATEEVVERLEPATGEDADAAVARAKKAFPKWRDVEPS
                     DRSRLLRRLADALDAEKEKLAQLESKNTGKPIGDSRGEMGMVVETFHYYAGMPERLLG
                     DSIPVSGGVDVTFREPLGVVGLIVPWNFPLTIASWKMAPALAAGNTLVLKPAGLTPLT
                     AVEFERIAREAGIPEGVVNVVVGPGSEVGQRLVENPDVAKVAFTGSTEVGRRIAEGAA
                     GTIKRVTLELGGKSANVVFADADLEKAASEAPAAVFGNAGQDCCARSRILVERKVFDD
                     FLSLMQPAVEGFRVRDPFDEGTDMGPLISADHRETVSSYVPEDAPVAIKGSAPQGSGF
                     WYPPTVLAPLSNNDKAAREEIFGPVVVVIPFDDEEDAVRQANDTIYGLSGSIWTENGA
                     RALRVARRMETGVISVNSNTSVRVATPFGGMKQSGVGRELGPHALDYYTEIKNVYIST
                     EED"
     gene            complement(2291..3013)
                     /locus_tag="VFT03_10370"
     CDS             complement(2291..3013)
                     /locus_tag="VFT03_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4848591.1"
                     /translation="MSGFARPTIGITAATEDVSYGAWDEVPAFISPASYVQAVQRAGG
                     RPILLLPDPEDAEDPNGVLDLVDALILTGGAGDVNPALYGQERHPETGPIQDERDAYE
                     FALARGAVERGIPTLGICRGMEVLNVVYGGSIEQHLPDMLGHEEHRHTPGTFADHEVR
                     LAPGSLAARAAGSESTPVKSHHHQGIKEVGSGLEVTGWAAEDDAVEALEDPSCPFVLG
                     VLWHPEEDEKSQLIKALVSEVS"
BASE COUNT          455 a         1049 c         1042 g          582 t
ORIGIN      
        1 gtacttggcg atgcgcgctt tgatcgagcc cgaggggttg aggtactcga gcttgcaata
       61 gataccttcg atctcgagca tcggcgtgac cccgatcgcg tcgagcacgc tctccgagat
      121 gccgccgtga cgcgctcctt gtgtctgcat ctggacctcc tattctggtg taacgtaagc
      181 cccggagagg cccccgtcaa ccaggaaggt cgaggccgtg acgtatgacg actcgtcgga
      241 ggccaggaag agggcgccgt tggcgatctc ggtcgcctcg gcgaagcggc ccatcggtag
      301 atggaccagc cgcctctcgg ccttctccgg gtccttggcg aagagctcct gcaagagcgg
      361 tgtgttcacc ggccccggac acagggcgtt gacgcgcact ccctgccggg cgaactgcac
      421 gccgagctcg cggctcatgg cgagcacgcc gccctttgat gcggtgtagg agatctggga
      481 cgcagccgcc cccatcaccg ctacgaaaga agccgtgttg ataacggacc ctccgccgcg
      541 ctcgagcagg tacggtatcc cgtacttgca gcacaggtag acgctcttga ggttcacgtc
      601 ctgcacgcgc tgccaggctt cgagctcggt ctccaggatc gagtcgtcgt ccggcgggga
      661 gatgcccgcg ttgttgaaga gcacgtcgag cccgccgaag cgctctgagg cttccctgta
      721 catcccctgc acgtcgtccg ggttcgtaac gtcggcgtgg atgtagaggc cgtctatctc
      781 cgcggccgtc tctttgcccg gctcgtctgc gaggtcggcg acgcataccc tcgcgccttc
      841 ttcggcgaag cgcttggccg tctcgcgtcc gatgccgctc gccccaccgg tgatgaccgc
      901 gaccttccct tcgagacgtc ccggcatgtc agtcctcctc ggtggagatg tagacgttct
      961 ttatctcggt gtagtagtcg agggcgtgcg ggccgagctc gcgtccgacg ccggactgct
     1021 tcatcccgcc gaagggcgtc gcgacccgga cggaggtgtt ggagtttacg gagatgacgc
     1081 cagtctccat gcgcctcgcg acgcgcaggg cgcgggcgcc gttctccgtc cagatcgagc
     1141 ctgagaggcc gtagatggta tcgttcgcct gccgaacggc gtcctcctcg tcgtcgaagg
     1201 ggatcacgac gacgaccggg ccgaagatct cctctctcgc cgccttgtcg ttgttggaga
     1261 ggggcgcgag caccgtcggc ggataccaga acccgctccc ctgcggggcg gagcccttta
     1321 tcgccacggg cgcgtcctcg gggacgtagg aggagacggt ctcgcggtgg tccgcggaga
     1381 tgagcgggcc catgtcggtt ccttcgtcga agggatctcg gactctgaag ccctcgactg
     1441 ctggctgcat cagggagagg aagtcatcga agaccttcct ctcgaccagg atgcgcgagc
     1501 gggcgcagca gtcctgcccc gcgttgccga agacggcggc cggagcctcg ctggcggcct
     1561 tctcgaggtc ggcgtcggcg aagactacgt tcgccgattt accgcccagc tccaacgtca
     1621 cccgctttat ggtgcctgcg gcgccctcgg cgatcctcct ccctacctcg gtagatcccg
     1681 tgaatgctac cttcgcaaca tccggattct cgaccaatcg ctgcccgacc tcgctacccg
     1741 gccctacgac gacgttcacg acgccctcgg ggattccggc ttctctggct atgcgctcga
     1801 actcgacggc ggtgaggggc gtgagccctg ccggcttgag gacgagggtg ttcccggctg
     1861 cgagcgcagg ggccatcttc cagctcgcta tggtaagcgg gaagttccac gggacgatga
     1921 gcccgacgac gcccagaggc tcgcggaagg tgacgtccac gccgcccgag acggggatgc
     1981 tgtcgccgag gaggcgctcc ggcatcccgg cgtagtaatg gaaggtctct acaaccatcc
     2041 ccatctcgcc gcgcgagtcg ccgatgggct tgccggtatt cttcgactcc agttgcgcca
     2101 gtttctcctt ctcggcgtct agggcgtcgg cgagacgcct gaggaggcgc gagcggtccg
     2161 agggttcgac gtccctccac ttcgggaagg ccttcttcgc gcgggccaca gcggcgtcgg
     2221 cgtcttcccc ggtcgccggt tcgagcctct caaccacctc ttcggtggcg gggttcagga
     2281 cttcgagcat ctaggagacc tccgagacta gggcttttat gagctggctc ttctcgtcct
     2341 cctcggggtg ccagagcacg ccgagcacga acgggcagct cgggtcttcg agcgcctcga
     2401 ccgcgtcgtc ttcggccgcc cagccggtga cctcgaggcc gctccccacc tcctttatac
     2461 cctggtggtg gtgcgacttg accggcgtgc tctcggagcc ggccgcgcgg gcggcgagcg
     2521 agccgggggc caggcgcacc tcgtggtcgg cgaacgtgcc gggggtgtgg cggtgctcct
     2581 cgtggccgag catgtcaggg aggtgctgct cgatgctgcc gccgtatacg acgttcagga
     2641 cctccatccc gcggcagatg ccgagcgtgg gtatgccgcg ctcgacggcg ccgcgcgcca
     2701 gagcgaactc gtaggcgtcg cgctcgtcct ggatcggacc cgtctcgggg tggcgctcct
     2761 ggccgtagag agccgggttc acgtcccccg ccccgccggt gaggatcagg gcgtccacga
     2821 gatccagcac cccgttcggg tcctcggcgt cctcggggtc ggggaggagc aggatgggcc
     2881 ttccgccggc tctctggacg gcctgcacgt agcttgcagg actgatgaag gcagggacct
     2941 cgtcccaggc gccgtagctc acgtcctcgg tggccgccgt tattcctatg gtgggcctgg
     3001 cgaagccact cataggcgct cgaaggatcg gtagcgctcc cagtctgtga cggcggcctc
     3061 gaaggcctcg atctcgaccc gcgccatgtt caggtagtgc tcgacgacct cctcgccgaa
     3121 cgcatctc
//
LOCUS       DASTUV010000513         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_909586, whole genome shotgun sequence.
ACCESSION   DASTUV010000513 DASTUV010000000
VERSION     DASTUV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909586"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFT03_10375"
     CDS             <1..186
                     /locus_tag="VFT03_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848592.1"
                     /translation="SQVVLIVTGHVLAVYLGHVVSLRFFGSPQLALRSQLPLLALMIL
                     YTIFSLWILSQPIVSRG"
     gene            complement(298..921)
                     /locus_tag="VFT03_10380"
     CDS             complement(298..921)
                     /locus_tag="VFT03_10380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU4848593.1"
                     /translation="MEAEQFTVRLAGPEEDPAIAALVVEGFIDKFRPVFGRRMDRSVK
                     IMERWIGLEHASGGVTSLVIEGYGAGEIAGSVGVRTADSREDVLARGLWRTLERNLGL
                     PRALWATTLLSYPRYSSISSEAYIERLVVSPDFRRQGMARRLLAAAEDLARDAAKATV
                     GLHVSANNLAALRLYEGEGYEEISRQRSFLTNHFLNIRDWLYLKKEL"
     gene            complement(952..1749)
                     /locus_tag="VFT03_10385"
     CDS             complement(952..1749)
                     /locus_tag="VFT03_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4848594.1"
                     /translation="MGAIRVPSPRGAFRVWQRNLTIFRKYWKSIMFPNFIEPLLYLAA
                     LGLGLGAFIQQGGINGQSYIQFIAPGLLASNAMFAASFESTFNTFVKFEINKIYDAII
                     TTPINAEDVVAGEYLWAGTRAALYGTAFLAVLTVLGFAFDEPLITSFWAVLIPPTLLV
                     IGIMFSVMGTLFTSLIQRIDFYAYYFTLVVTPLFLFSGIFFPVEDFPAPVPQIAWFTP
                     LYHAVNVCRALATGPTPAVLVDVAWILVFTLALALVPVQIMRRRLIG"
     gene            complement(1749..>2405)
                     /locus_tag="VFT03_10390"
     CDS             complement(1749..>2405)
                     /locus_tag="VFT03_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848595.1"
                     /translation="DDLKVEENLLVYGRYYDLPRKVALQRAEELLDFVELTEKAESQV
                     EQLSGGMKRRLLIARALINDPEIVVLDEPTTGLDPQARHLVWDRLRALTVEGKTLVLT
                     THYMEEAARLCDRLVIMEDGLIIAEGSPKELVEKYVSPQVLEFRGSPEELERLEPVVE
                     AVADAVERTGDTLLAFTTEAEELMEQVRASGVEVENTVYRQAALEDVFLRLTGRRLIE
                     "
BASE COUNT          439 a          769 c          768 g          429 t
ORIGIN      
        1 tctcaggtcg tactgatcgt gaccgggcac gtccttgccg tgtacctggg gcacgtggtc
       61 tcgctgcgct tcttcggctc gccacagctg gctctgagga gccagctccc gctactcgcg
      121 cttatgatcc tctacacgat ctttagcctc tggatcctct cccagcccat agttagtcga
      181 ggatgaaggc ggcgaggaat acccttcgcg gcgttgccca aaaagcgttc gaagatagcc
      241 tgccagcgac cgcggcgtag gggcggttcg cgaaccgtca tggctaccca atgcgctcta
      301 gagctctttc ttcaggtaca gccagtccct gatgttcagg aagtgattcg tcaggaagga
      361 gcgctgccgg gagatctcct catacccctc tccctcgtag agccgaagcg cggcgaggtt
      421 atttgcgctg acgtggaggc cgacggtcgc cttcgcggca tcccgcgcga ggtcctcggc
      481 ggccgcgagc aggcggcggg ccatcccctg acgacggaaa tcaggcgaga cgacgaggcg
      541 ctctatgtag gcctcggagg agatcgagga gtagcggggg taagagagta gggtcgtggc
      601 ccacaaggcg cgtgggaggc ccaggttgcg ctcgagagtc cgccagagtc ctcgggcgag
      661 aacgtcctcc ctggagtcgg ctgtgcggac gccgacgctg ccggcgatct ccccggcgcc
      721 gtagccttcg atcaccagcg acgttacgcc gcccgaggcg tgctcgagac cgatccacct
      781 ctccatgatc ttgaccgaac gatccatccg cctgccgaac acagggcgga acttgtcgat
      841 aaatccttcg acgacgagcg cggctatcgc cgggtcctcc tctggccccg cgaggcgcac
      901 ggtgaattgt tcggcctcca ttccagagct atcctaccag aactcgggcc ctcagccgat
      961 aaggcgccgg cgcatgatct ggaccggaac gagggctagc gcgagggtga aaacgagtat
     1021 ccacgcgacg tccacgagca ctgcgggcgt cgggcccgtg gccagcgccc ggcagacgtt
     1081 gaccgcgtgg tagagcggcg tgaaccacgc gatctggggc accggcgcgg ggaagtcctc
     1141 taccgggaag aagatgcccg agaagaggaa gagcggcgtg acgacgaggg tgaagtagta
     1201 ggcgtagaag tcgatgcgct gtatcaggct ggtaaaaagc gtccccatga cgctgaacat
     1261 gatgccgatc acgagcaacg ttggcgggat gagaaccgcc cagaacgagg tgatgagcgg
     1321 ctcgtcgaag gcgaagccga ggacggtgag gaccgcgagg aaggcggtgc cgtaaagcgc
     1381 ggcgcgcgtc cccgcccaca ggtactcgcc ggcgacgacg tcctcggcgt tgatcggggt
     1441 cgttatgatc gcgtcgtaga tcttgtttat ctcgaacttg acgaaggtgt tgaaggtgct
     1501 ctcgaacgac gcggcgaaca tggcgttgct cgccagcagc cctggcgcga tgaactggat
     1561 gtagctctgc ccgtttatcc cgccctgctg gatgaacgcc cccaggccca accccagcgc
     1621 cgccaggtac agcaggggct cgatgaagtt ggggaacatg atgctcttcc agtacttgcg
     1681 gaagatagtc aggttgcgtt gccatactct gaacgccccg cgcggggagg gcaccctgat
     1741 agcacccact actcgatgag cctcctccct gtgagccgca ggaagacgtc ctcgagcgcc
     1801 gcctgcctgt acacggtgtt ctccacctcc acccccgagg cgcggacttg ctccatgagc
     1861 tcctcagctt ccgtggtgaa cgcaagtaac gtatcacctg ttcgctcgac ggcgtccgcg
     1921 accgcctcga cgaccggctc gagcctctcg agttcctcgg gagagccgcg gaactccagc
     1981 acctgtgggc tgacgtactt ctccaccagc tctttgggcg atccctcggc gatgatgagc
     2041 ccgtcctcca tgataacgag ccggtcgcac agccgagctg cctcctccat gtagtgggtc
     2101 gtgaggacca gcgtcttacc ctcgacggta agcgcgcgca gccggtccca caccaggtgc
     2161 ctggcctgcg ggtcgaggcc agtggtcggc tcgtcgagca ccacgatctc agggtcgttt
     2221 atgagcgccc gcgctatgag gagccgccgc ttcatgccgc cagagagctg ctccacctgc
     2281 gactctgcct tctctgtgag ctccacgaag tcgagtagct cctcggcccg ctgcagcgcc
     2341 accttgcgcg gcagatcgta gtaccgaccg tagacgagca ggttctcctc gaccttgagg
     2401 tcgtc
//
LOCUS       DASTUV010000514         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_302067, whole genome shotgun sequence.
ACCESSION   DASTUV010000514 DASTUV010000000
VERSION     DASTUV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302067"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFT03_10395"
     CDS             complement(<1..823)
                     /locus_tag="VFT03_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU4848596.1"
                     /translation="MSVVHRFEGLEEAVREGFGEKIVALRRDIHREPELGFDTEKTAE
                     KVLAALDGLPLDIQTGVAENGIVATLKGERDGPTVGLRADMDALPIHEETGFPFASQT
                     AGTMHACGHDGHTSMLVGAAHALSGMRERLSGNVRFFFQPAEEGGGGGEVMVEEGVAD
                     DVSSIFALHLWPGLPFGTAATKAGPIMAAADAFEMTVRGAGGHGAMPHLTADAIAMAA
                     QIVTALQTVVSREVDPVQPAVLTVGEIGAGSAYNIIPETARLGGTVRTLNEGLRRK"
     gene            complement(881..1855)
                     /gene="gluQRS"
                     /locus_tag="VFT03_10400"
     CDS             complement(881..1855)
                     /gene="gluQRS"
                     /locus_tag="VFT03_10400"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEU4848597.1"
                     /translation="MGEHREVGRPSPRLPRADAGPHGADGRFAPSPTGPLHVGNLRTA
                     LLAWLFARSEGARFLVRVEDLDRSRVQEGMEEAQLTDMWALGLDWDGPVVRQSERLAL
                     YEEAITGLDGEGLLYPCYCTRAEIRASVSAPHGILAADRYSGTCRELTARERAEKEAS
                     GRPPALRVRAENIRVSFEDRLLGPYEGEVDDFVVRRNDGAPAYQLAVVVDDAMQDIDE
                     VVRGADLLDSTPRQIHLHRVLGLPSPGYAHVPLVLGPDGRRLAKRHGAVTLADREALG
                     ESPREVLAWMARSLGLAAAGESPAPADLLARFDPARLPLDPTVWSPAK"
     gene            complement(1834..>2188)
                     /locus_tag="VFT03_10405"
     CDS             complement(1834..>2188)
                     /locus_tag="VFT03_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4848598.1"
                     /translation="RHAIEVGGLRAGERLPTVRRLAEDLTIAPNTIVRAYNELQRMGY
                     VVSRPGIGTVVAEGIEEVARERRVEGIFERLRVLVRDAAALGISEDELWASLDAEFER
                     IPRDNLQEHWESTEK"
BASE COUNT          337 a          816 c          673 g          362 t
ORIGIN      
        1 tcttgcgtct gaggccctcg ttaagcgtgc gcaccgtccc gcccagccgt gccgtctcgg
       61 ggatgatgtt gtaggcgctg ccagccccga tctcccccac tgtaagcacc gcgggctgga
      121 ccgggtccac ctcccgcgag accaccgtct ggagcgccgt cacgatctgg gctgccatcg
      181 cgatagcgtc cgccgtgagg tgcggcatgg cgccatgccc gccggcgccc ctcaccgtca
      241 tctcgaaggc gtcggcggcg gccatgatcg gaccggcctt cgtcgccgcc gtcccgaacg
      301 gcagcccggg ccacaggtgc agcgcgaaga tggaagagac gtcgtccgct accccctcct
      361 cgaccatgac ctcgccccca cccccgccct cctcggccgg ctggaagaag aacctgacgt
      421 tgccgctcaa cctctcgcgc atccccgaga gggcgtgcgc cgccccaacg agcatgctag
      481 tgtgcccgtc gtggccgcag gcatgcatcg tgccggctgt ctgcgaggcg aaaggaaacc
      541 ccgtctcctc gtggatcggc agcgcgtcca tgtccgccct caaccctacc gtcggaccgt
      601 ctctctcgcc tttcaaggta gccactatcc cgttctcggc cacgccggtc tgtatatcga
      661 ggggtagacc atccagagca gcgagcacct tctccgccgt cttctccgtg tcgaagccga
      721 gctcgggctc cctgtggatg tcgcgcctga gcgcgacgat cttctcgccg aagccttcgc
      781 gcaccgcttc ctcgagacct tcgaacctgt ggaccacgct catctctggc tcccttccac
      841 gcggatttct cagtctacag cgtttgccaa cgcgcggggc tcacttagca ggcgaccaga
      901 ccgtcggatc tagcggcagt cgagcaggat cgaagcgcgc gagcaggtcg gcgggagccg
      961 ggctctcgcc tgccgccgcg agtccgagcg agcgagccat ccaggcgagg acttctcgcg
     1021 ggctctcgcc gagagcctct ctatcggcga gcgtcaccgc cccatgccgc ttggccaacc
     1081 gccgcccgtc gggtccgagc acgagcggca cgtgggcgta gccgggcgag ggaaggccga
     1141 gtacccgatg gaggtggatc tggcgcggcg tcgagtccag gaggtcagcc ccccgcacca
     1201 cctcgtctat gtcctgcatc gcgtcgtcca ccacgacggc gagctggtag gcgggggcgc
     1261 cgtcgttgcg cctcacaacg aagtcgtcta cctcaccctc atagggccca agcagacgat
     1321 cctcgaacga tacgcggatg ttctccgcgc gcaccctcag ggcgggcgga cggcccgaag
     1381 cttccttctc ggcacgctct cttgccgtca actcgcggca ggttccggag taccggtcgg
     1441 cggcgaggat accgtgcggc gccgagaccg aggcccggat ctccgcccgc gtgcaatagc
     1501 aggggtagag aagcccttcg ccgtcgagtc cggttatcgc ttcctcgtag agcgccagcc
     1561 gctctgactg gcgcacgaca ggtccgtccc agtcgagccc aagggcccac atatccgtca
     1621 gttgcgcttc ctccatccct tcctggacgc gcgagcggtc gaggtcctcg acccgcacga
     1681 ggaaacgcgc gccctcagaa cgggcgaaga gccaggcaag cagagcggtc cgcaggttgc
     1741 cgacgtggag cggtcccgta gggctgggcg cgaagcgccc gtcagcgcca tggggaccag
     1801 cgtccgcgcg cggaagacgc ggcgacggtc ggcctacttc tctgtgctct cccaatgctc
     1861 ctggaggtta tcccgaggta tgcgctcgaa ctccgcgtcc agactggccc acagctcgtc
     1921 ctcgcttatc ccgagggccg ccgcgtctcg taccagaacc cgcagacgct cgaagatccc
     1981 ctcgaccctt cgctcgcgag cgacctcctc gataccctcc gctaccaccg tcccaatccc
     2041 gggccggctc acgacgtacc ccatgcgctg cagctcgttg taagccctga cgatcgtgtt
     2101 gggtgctatc gtgaggtctt cagccagccg gcggacggtc ggcaaccgct ccccggctct
     2161 caagcccccg acctcgatcg cgtgccgt
//
LOCUS       DASTUV010000515         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_139963, whole genome shotgun sequence.
ACCESSION   DASTUV010000515 DASTUV010000000
VERSION     DASTUV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139963"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /gene="pilM"
                     /locus_tag="VFT03_10410"
     CDS             <1..1210
                     /gene="pilM"
                     /locus_tag="VFT03_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEU4848599.1"
                     /translation="GRTLDLTGAAPRSLAGFDPGGVGVMDRLGGLGRAGSRTIGLDID
                     RGALKAVQISGSAGDLTLRHVGYHRLPPGAVVDGEVADVEVVGAEIQEFWSSHSFKGK
                     SVNLGVANGRVVVRLLDFPRMEAEDLKSAITFEAQDHIPMPLDEAVLDYVVLGPQAEG
                     SDLDRILLVAAHKDMIGGYTAALRAGGLKPAGIDVKALALTRSALPETFFDEGATLLL
                     DIGTELTNLAITQGSNPTLTRFIPSGLDRFVEAVSEAADLPEEQAEKEALNPRVRLGS
                     DPEVTDAPGLEEEEEEEEEEFDSALVYDVRRGLEEAVQALAEDVHRSIEYHHSQPGTS
                     EVSQAFVSGEGALIPGLDAYLRELLGVSTHRANPVAKLGSNKSNVSDEQLTAMEPVLA
                     VALGLAMEDE"
     gene            1207..1929
                     /locus_tag="VFT03_10415"
     CDS             1207..1929
                     /locus_tag="VFT03_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848600.1"
                     /translation="MRRINLLPPEERRRPAERLRGGAPAILLIVGATALIFMVGVYLF
                     FLFRLNDVEDEIAGLDQQIADQNAQLAELEPYRDLQAQLDAKKPVADGIYSTRFPWDQ
                     FLQGLAFVIPESTALDTFTAEAAPVSIEAQPEQPLEPPGAITFTGIALPRYQNVADFV
                     VQINDLRYLSNAELSSAQLDRDTFAQPAINFEVASELITVAGENGTEVRLDGGSSTQA
                     ADTGVQEERYQARVDRPGSERW"
     gene            1926..2630
                     /gene="pilO"
                     /locus_tag="VFT03_10420"
     CDS             1926..2630
                     /gene="pilO"
                     /locus_tag="VFT03_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU4848601.1"
                     /translation="MSRTDRNILILGLLIIVLVVVGYYFLLLSPLLGTLDARAQERST
                     KESELASLQQEVDQLEAAKRNAPETERQLLELAKRVPTQPEIPTLVVQIEDVADASGV
                     SQLSIEPGTPGPPPGGGNFSVVPVTMSFEGTYEQMQDFLLRTRNLARLVTVNSVTYCR
                     VVPLGTGEGTCNIEIPTAGETTTLPEIEEQLQVEIEAEVYFQPSVEPSNVPAGTPPVA
                     PAPPEETTPEETTGAQ"
     gene            2620..>2936
                     /locus_tag="VFT03_10425"
     CDS             2620..>2936
                     /locus_tag="VFT03_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848602.1"
                     /translation="MLNRLREAMFGVGRRVSAVGSGFVDTFLRDNRVIPIVLALLALL
                     VFAWILADAFLGGGPAKEPVSNRAEVAQAQNPPTSDPSAPEVKNPDVDSYAAYQFKDP
                     FRQL"
BASE COUNT          543 a          956 c          958 g          479 t
ORIGIN      
        1 cgggaggacg cttgacctta ccggcgcagc tccgaggagc ttggccgggt tcgatccggg
       61 aggtgtgggg gtcatggacc gcctgggcgg cttaggacga gctggctccc ggaccatcgg
      121 gctggacata gaccgcggcg ccctcaaggc ggttcagatc tcggggagcg ctggtgatct
      181 cacgttgcgg cacgtcgggt accacaggct gccccccggc gcggtggtcg acggcgaggt
      241 cgccgatgta gaggtcgtcg gtgcggagat ccaggaattc tggtcctccc actctttcaa
      301 ggggaagtcg gtcaacctcg gcgtcgcgaa cggcagggtg gtcgtgcggc tactcgactt
      361 cccgcgcatg gaagcagagg atctcaagag cgccataacc ttcgaggccc aggaccacat
      421 ccccatgccg ctggatgagg ctgtcctcga ctacgtggtg ctcggtccgc aggccgaggg
      481 ttccgacctg gatcgcattc tactcgtggc cgcgcacaag gacatgatag gaggctacac
      541 tgctgcgttg cgggcgggcg ggctcaagcc cgcagggata gatgtcaagg cgctcgcgct
      601 aacgcgctcc gcgttacctg agacgttctt cgacgagggg gccacgctcc tcctcgacat
      661 cggcacggag ctcaccaacc tcgcgatcac acaggggagc aatcccaccc tgacccgatt
      721 cattccctcg gggcttgacc gcttcgtcga ggccgtctcg gaggccgcgg atctccctga
      781 agaacaggcc gagaaagagg cgctgaaccc gcgagtcagg ctcggctccg acccggaggt
      841 aacggacgcc ccgggcctgg aggaggaaga ggaggaagag gaggaggagt tcgattccgc
      901 gctcgtctac gacgtgcggc gcggcctcga ggaagcggtg caggcgctcg ccgaggacgt
      961 gcaccgctcc atcgagtacc atcactccca gccggggacg agcgaggtgt cgcaggcgtt
     1021 cgtctctgga gagggcgcgc tcatacccgg tctcgacgct tacttgcgcg agttgcttgg
     1081 cgtctcgaca caccgcgcca acccggtggc aaagctcggc tccaacaagt ccaacgtctc
     1141 cgacgagcag ctcacggcga tggaaccggt gctggcggtg gctctgggcc tcgcgatgga
     1201 ggacgaatga ggcggataaa cctcctcccg cccgaagagc gccgccggcc agcagagagg
     1261 ctgcgcggcg gggcacccgc gatactcctc atcgtcgggg ccacggcgct catctttatg
     1321 gtcggggtgt atttgttctt cctcttccgg ctcaacgatg ttgaggatga gatcgcgggg
     1381 ctcgaccagc agatagcaga tcagaacgcg caactggccg agctggagcc ttacagggac
     1441 ctgcaggcgc agctcgacgc caagaagccc gtggccgacg gcatctacag tacccggttc
     1501 ccgtgggacc agttcctgca aggcctcgcc ttcgtcatcc cggagagcac ggcgctcgat
     1561 accttcaccg cagaggcggc tcctgtaagc atcgaggcgc agcccgagca gcctctcgaa
     1621 cctcccggcg cgatcacctt caccgggata gccctgcccc gctaccagaa cgtggctgac
     1681 ttcgtcgtgc agattaacga cttgcgctac ctgtccaacg cggagctcag ctcagcccag
     1741 ctcgatcggg atacgttcgc gcagccggcg atcaacttcg aggttgcctc ggagctgatc
     1801 acggtggccg gagagaacgg gaccgaggtc agattagacg gcgggtcgtc cacgcaagca
     1861 gccgacacag gcgtacagga ggagaggtac caggcccgcg tggaccgacc cgggagcgag
     1921 cgctggtgag ccgcaccgac cgaaacatcc tcatcctggg actcctgata atcgtgctcg
     1981 tcgtcgtcgg ttactacttc cttctcctga gtccgctcct gggcaccctg gacgcgagag
     2041 cccaggagcg ctccaccaag gagtccgagc ttgcgagcct gcagcaggag gtcgaccagc
     2101 tcgaggcggc caagcgcaac gccccggaga ccgagcgcca gttgctcgag ctcgccaaaa
     2161 gggtgcctac ccagcccgag atacccacgc tcgtagtcca gatagaggat gtggctgacg
     2221 cctccggggt tagccagctc tcgatcgagc ccggaacccc ggggccgccg ccggggggtg
     2281 gcaacttttc ggtggtaccc gttacaatgt ctttcgaagg aacttacgag cagatgcagg
     2341 acttcctgct ccgaacgcgc aatctggccc ggttggtgac cgtgaactcg gtcacctact
     2401 gtcgcgtagt tccgctcggg acgggagagg ggacgtgcaa catcgagatc cccacggcgg
     2461 gggagaccac cacattgccg gagatagagg agcagctgca agtggagata gaggccgagg
     2521 tgtacttcca accctcggtc gagccctcta acgtcccggc agggaccccc cccgtagccc
     2581 ccgccccacc ggaggagacg acaccggagg agacgacggg tgctcaatag gttgcgggag
     2641 gctatgttcg gcgtgggacg gcgggtgtcg gcagtcggct ctgggttcgt cgacaccttc
     2701 ctgcgcgaca acagggtaat accgatcgta ctggccctgc tcgcgctgct cgtcttcgcc
     2761 tggatcttgg ctgacgcatt cctcggcgga ggccccgcca aggagcctgt ctcgaaccgg
     2821 gccgaggtcg cccaggcgca aaacccccca acctccgatc cctctgcccc cgaggtaaag
     2881 aaccccgacg tagactccta cgccgcctac cagttcaaag atcccttccg ccagct
//
LOCUS       DASTUV010000516         5078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_378271, whole genome shotgun sequence.
ACCESSION   DASTUV010000516 DASTUV010000000
VERSION     DASTUV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378271"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFT03_10430"
     CDS             complement(<1..245)
                     /locus_tag="VFT03_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848603.1"
                     /translation="MMRVFNRIVMILLFAGLFVLGVYTVVYSFDLFGYRLEDLPRALG
                     LSGFYRNLETFVSNVENGSLSVLAVILLVAIAVLGLIL"
     gene            complement(242..535)
                     /locus_tag="VFT03_10435"
     CDS             complement(242..535)
                     /locus_tag="VFT03_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848604.1"
                     /translation="MAEELELARAAAQAALATDGVYSMGRGRYVEAATYEGNEKVSGV
                     VVKPDEVEIHIVARYPLPSPIPEISRGIIERVAPRAGDRRIVVVVDDLEVVAS"
     gene            complement(566..1024)
                     /locus_tag="VFT03_10440"
     CDS             complement(566..1024)
                     /locus_tag="VFT03_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4848605.1"
                     /translation="MTDQSSSPLQTERGSTSISDSVVSKVAGIAAQEVEGIRMGSGAS
                     QTVGGFIGSITGGSGPQTAGVSVEVGQEEAALDLTLTAEYGKSIPQLAEAVRRNVINR
                     VESLIGLRVTEVNITVANVFFPQQEQEQQRQQELEQQRREQDAQEQQRVQ"
     gene            complement(1053..1304)
                     /locus_tag="VFT03_10445"
     CDS             complement(1053..1304)
                     /locus_tag="VFT03_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU4848606.1"
                     /translation="MVWIVVGLIAGILAKWVMPGPDPGGVVLTALIGIVGAIIGGWVL
                     GWLGFGGSAGSLSLESILTATLGAIILLAIYRLLTRRTV"
     gene            complement(1467..2849)
                     /locus_tag="VFT03_10450"
     CDS             complement(1467..2849)
                     /locus_tag="VFT03_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU4848607.1"
                     /translation="MGDGILGGFFRFSERGANFRTEVLAGLTTFMTMAYIVFVNPAIL
                     GTEGTGLPITGVFFATCVAASAASIAMGVFANYPLALAPGLGINAIVAFTLILELGLS
                     WQQAMAVIIVEGLLVTVLVLTNLREAVMNAIPMSLKLAIAVGIGLFIAFIGLKNAGIV
                     VQNPDTYLALGDFTQGPVLLALFGLVLSLALVARGMRGGILIGILLTGVVGMVFGIVP
                     LPGGVVDFRFDTSTIGGGVLAVPEVLRISLVPVIFALFMTDFFDTMGTVIAVGQEAKL
                     LDESGRPPNLKSILLVDSLAAVGGGATGASSVTSYIESGAGVAEGGRTGLVSVVVGLL
                     FLLALPFSPLVSVFGGSIPVPGPQGEGQIFVSPVTAPALIVVGFLMMTAVRFIPWDDF
                     DEAIPAFLTILTLPLTFNISYGIAFGFISHVLIKLSRGKPGEVHPLHYVFAVLFALTF
                     VWPALQDMLS"
     gene            complement(2984..3655)
                     /locus_tag="VFT03_10455"
     CDS             complement(2984..3655)
                     /locus_tag="VFT03_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848608.1"
                     /translation="MDTHKTEQLPDYEGEHHGERGLGAWIRRNPWLLVRLFIVMVFTF
                     GASTTAANFSYGLEGQPTRLSIEQLQRGELPPGTQLGDYVEVRGTPDIGNDLERIGTR
                     ESKIAVSSRYSASYFYFRLEETGDSLLIQTAQGLPQGLENAGEREWRGKLETVGTVIF
                     HDTTQEGLEHAGLPHDESIPVVTTGDTPEYYRRIFPAYSAIIGLWLASLAWLVWKRNK
                     PFLGL"
     gene            3856..4488
                     /locus_tag="VFT03_10460"
     CDS             3856..4488
                     /locus_tag="VFT03_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848609.1"
                     /translation="MEGREAPERVPEYTVVGVINDGVELNQAVRELQELGVDRDDLTV
                     VLKREDMSEPEPFPEGTRYIVVPGDKRGLEVPIGFAIAFIVFGVLFAITTPAIGIPTL
                     MVFVSLAAILFVGSLTRVGVSPILIEMGAPSEEAGTWNDDFEMGKVLIFASTSERRLL
                     RPIREALQRAGAMYYIVDRRLEPRAVHQATLHRAGERGAGESVANRTGSA"
     gene            4510..>5078
                     /locus_tag="VFT03_10465"
     CDS             4510..>5078
                     /locus_tag="VFT03_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848610.1"
                     /translation="MAPSLALLLLAQKGLAVDLSSAFSEIGGVRFVTGLTMLIHMFFA
                     ELFVGFAIAAPVLQAWGARTGSPRMDRLAHSMARFNVLTFSTGATFAVLFVVILVGLY
                     PRVTTSLFSHFFYFIALAMLSMVLALWGMYSYYYKWDRYSVLHKKRHIALGFTMGFFI
                     WIWMMIMTGIDTYMVTGGGPGSPPLSAGN"
BASE COUNT          898 a         1654 c         1622 g          904 t
ORIGIN      
        1 aggatcaggc cgagtacggc gatggcaacg agcaagatca ccgccagcac cgacaggctg
       61 ccgttctcga cgttgctcac gaaggtctcc aggttgcggt agaagccgga gagccccaga
      121 gcgcggggca ggtcttccag cctgtagccg aagaggtcga agctgtagac aaccgtgtag
      181 acgcccagca cgaagaggcc ggcgaagagc agtatcataa ctatcctgtt gaagaccctc
      241 atcatgaggc caccacctcg aggtcatcga ccaccacgac gatcctccgg tcgccggccc
      301 ggggggccac gcgctcgatg atgccgcggg atatctctgg tatggggctg ggcagcgggt
      361 agcgggccac gatgtggatc tctacctcat cgggcttgac taccaccccg gagaccttct
      421 cgttgccctc gtaggtcgca gcctccacat agcgcccgcg ccccatggaa tacaccccgt
      481 ctgtggcgag ggccgcctgg gcggcggccc tcgccagttc cagttcttca gccaagatca
      541 gcctgtcctt cgggcgggca cggccttact ggacccgttg ctgttcctgc gcatcctgct
      601 cccggcgctg ctgctccagc tcctgctggc gctgctgctc ctgctcctgc tgcgggaaga
      661 agacgttggc aaccgttatg ttcacctcgg tcacccgcag gccgatcagg ctctccaccc
      721 ggttgatcac attgcgcctg acggcctcgg cgagctgggg gatggacttg ccgtactcgg
      781 ccgtgagcgt caggtcgagg gccgcctctt cctggccgac ctcgaccgag acgcccgcgg
      841 tctgggggcc gctgccaccg gtgatgctgc cgatgaagcc gcccacggtc tgcgaggcgc
      901 cgctgcccat cctgatgcct tccacctcct gggccgcgat cccggctacc ttcgatacca
      961 cgctgtccga gatgctcgtg ctgccgcgct cggtctgtag cgggctactg ctctggtcgg
     1021 tcatgcgtct ttctcctttc gtgctgcgtg ctttataccg tccggcgcgt gagcagccgg
     1081 tagatggcga gcaggatgat ggcgcccaac gtggccgtca gtatggactc gaggctgagg
     1141 ctccccgcgc tcccaccgaa gccgagccat ccgagcaccc atcccccgat aatggcgccc
     1201 acgatcccga tcagagccgt gagcacgaca ccccccgggt ccggtcctgg cataacccac
     1261 ttcgccagga tgcccgctat gagcccgacc actatccaca ccacgatggc cccgatgctt
     1321 atggcttttc acctccccac ctccaccgcg cctgtgccgg acgacacctg gtggaatata
     1381 taacagtcgg cgtgctccgc gctactatcg ccgattctac ccccgccgga gcggccataa
     1441 acaccgcgta tctgtcgggg gttctcctac gagagcatgt cctgcagggc gggccagacg
     1501 aaggtcagcg cgaagagcac ggcgaagacg tagtgcagcg ggtgcacctc gcccggcttg
     1561 cccctggaaa gcttgatcag cacgtgggag atgaaaccaa acgcgatgcc gtaggagatg
     1621 ttgaaggtca gcggcagcgt caggatggtg aggaaggcgg gtatcgcctc gtcgaagtcg
     1681 tcccacggga tgaagcggac ggcggtcatc atcaggaaac cgacaactat caatgccggg
     1741 gccgtcaccg gagagacgaa gatctgaccc tctccctgcg ggccagggac aggtatcgag
     1801 ccgccgaaga ccgagacgag gggcgagaac ggcagcgcga gcaggaagag cagacctacc
     1861 accacgctca caagacccgt acggcctccc tcggcgacgc cggccccgct ctctatgtag
     1921 ctcgtcacgg agcttgcgcc ggtcgctccg ccgccgaccg cggccagcga gtcgacgagc
     1981 agtatgcttt tgaggttcgg cggcctgccg ctctcatcca gcagcttcgc ctcctggccg
     2041 accgctatca cggtgcccat cgtgtcgaag aagtccgtca tgaaaagcgc gaagatcacg
     2101 ggtacgagcg atatccggag cacctcggga accgcgagca ccccgccgcc gattgtggag
     2161 gtgtcgaagc ggaagtccac gacgccaccg ggcagcggca cgataccgaa gaccatgccc
     2221 acgacgccgg tgagcaggat gccgatgagt atgcccccgc gcatcccgcg cgctacgagc
     2281 gcgaggctga gcactagccc gaagagcgcg agcaagaccg gcccctgcgt gaagtcgccc
     2341 agagccagat aggtgtcggg gttctggacc acgatgccgg cgttcttcag gcctatgaag
     2401 gctatgaaca gcccgatgcc gaccgcgatg gcgagcttga gcgacatggg gatggcgttc
     2461 atcaccgcct cgcgcagatt cgtcaggacg agcaccgtga cgagcagacc ctcgacgatg
     2521 atcaccgcca tcgcctgctg ccacgagagc ccgagctcga gtatcagggt gaaggcgact
     2581 atggcgttga tgcccagccc gggggcgagg gccagcgggt agttcgcgaa gacccccatc
     2641 gctatgctag ccgccgaagc ggccacgcac gtcgcgaaga acacccccgt gatcggaagg
     2701 cccgtaccct ccgtgcccag tatcgccggg ttgacgaaaa cgatgtaggc catcgtcatg
     2761 aacgtggtca accccgcgag cacctccgtc cggaagttgg ccccccgctc ggagaacctg
     2821 aagaagccgc ccaagatccc gtctcccatc tgcaagctta tctgcaatgg aaatatagca
     2881 gcaacaggtg tcaggattca ggcattggac agcgcgtatg tcgcgttttg agggctttcg
     2941 gaggcgaacg agagcctctc ttcgaccccc tgatgcccgt agcctacaaa cccaggaagg
     3001 gtttgtttcg tttccagacg agccaggcga gggaggcgag ccagagcccg atgatggccg
     3061 agtaggcggg gaagatcctg cgatagtact ctggggtatc gccggtggtt accacgggta
     3121 tgctctcgtc gtgtgggagg ccggcgtgct ccagcccctc ctgggtggtg tcgtggaaga
     3181 tcacggttcc cacggtctcg agcttgcctc tccactctcg ctccccggcg ttctcgaggc
     3241 cttgcggcag gccctgcgcc gtctgtatga gcagggagtc ccctgtctcc tccaggcgga
     3301 agtagaagta gctcgcgctg tagcgcgagg agacggctat cttggactcc ctggtcccga
     3361 tccgctccag gtcgttcccg atgtcgggcg tgccgcggac ctcgacgtag tcgccgagct
     3421 gcgtgcccgg cggcagctcg ccgcgctgca actgctcgat gctcaggcgg gttggctgcc
     3481 cctcgagccc gtaggagaag ttggccgcgg tcgtcgaggc gccgaaggtg aacaccatga
     3541 cgatgaacag gcgcacgagg agccaggggt tgcgccggat ccaggccccc aggccccgct
     3601 ctccgtgatg ttctccctcg tagtcgggga gttgctctgt tttgtgggtg tccacgcccg
     3661 caagtttacc cgagcgcgcc gaagcggatg gtgacggttt cacacggcag ggccaatatc
     3721 ctggcgtgtg aaagagattc ccgcgggtgg tgggggcggt ctagtatgga gtggcgcgag
     3781 catgtatgct gttgaccggc aaaaaccgcc gcggaaggtt agggccgtta gccacctttg
     3841 tggaggtaga agagtttgga agggcgggag gcaccagagc gggtccccga gtacaccgtg
     3901 gtaggggtga taaacgacgg ggtcgagctc aaccaggccg tcagggaact ccaggagctc
     3961 ggggttgacc gcgacgacct gacggtggtc ctcaagcgcg aggacatgag cgagcccgag
     4021 ccttttcccg aggggacgcg ctacatagtg gtcccagggg acaagcgcgg gctcgaggtg
     4081 cccataggat tcgccatcgc gttcatcgtc ttcggggtgt tgttcgccat cacgacgccc
     4141 gccatcggga tcccgacgct catggtcttc gtctcgctgg cggcgatact cttcgtcggc
     4201 tcgctgaccc gggtcggggt ctcgccgatc ctcatcgaga tgggagcccc ctccgaggag
     4261 gccggcacct ggaacgacga cttcgagatg ggcaaggtcc tcatcttcgc ctcgacgagc
     4321 gagcggcgtc tgctcaggcc gatacgggag gccttgcagc gcgccggcgc gatgtactac
     4381 atcgtggacc ggcgtttgga gccccgcgcc gtccaccagg ctacgctgca ccgcgccggc
     4441 gagcgcggcg cgggcgagtc cgttgcgaac cgtaccggta gcgcctagga taggagttca
     4501 ggaatgacca tggcaccatc gctggctctc ctcctcctgg cccagaaggg cttggccgtg
     4561 gacctctcga gcgccttctc ggagataggc ggcgtccgct tcgtcacggg tttgacgatg
     4621 ctcatccaca tgttcttcgc ggagcttttc gtcgggttcg cgatagccgc gccggtcctg
     4681 caggcgtggg gcgcgcgcac ggggagcccg cggatggaca ggctcgccca ctccatggcg
     4741 cgcttcaacg tgctcacctt ctcgaccggc gccaccttcg ccgtcctctt cgtggttatc
     4801 ctcgtcggcc tctacccgcg ggtgacgacc tcgctcttct cccacttctt ctacttcata
     4861 gctctggcta tgctctcgat ggtcctcgcg ctgtggggca tgtactccta ctactacaag
     4921 tgggaccgct acagcgtgct ccacaagaag cggcacatcg ctctaggttt caccatgggc
     4981 ttcttcatct ggatctggat gatgatcatg accggcatag acacctacat ggttaccggc
     5041 ggagggccgg gaagtccgcc gctctccgcg ggaaacat
//
LOCUS       DASTUV010000517         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1303511, whole genome shotgun sequence.
ACCESSION   DASTUV010000517 DASTUV010000000
VERSION     DASTUV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303511"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFT03_10470"
     CDS             complement(<1..702)
                     /locus_tag="VFT03_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU4848611.1"
                     /translation="MSFGKVLIANRGEIAVRVILACRELGIRSVAVYSEADEKAMHRR
                     LADEAHPIGPAPAAESYLNIERLLEAIEASGADAVHPGYGFLAESAPFARAVESSGAV
                     WVGPPPEAMEAMGFKVRAKELARQAGVPTVPGYDGEEESEERLAEEAEALGFPVLVKA
                     SAGGGGRGMRALTRPQDFAEAVRGARREAEAAFGDGSVFLEKLIEDPRHVEVQVIGDA
                     EGNVIHLYERECSIQR"
     gene            complement(699..1490)
                     /locus_tag="VFT03_10475"
     CDS             complement(699..1490)
                     /locus_tag="VFT03_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU4848612.1"
                     /translation="MPETYENLRRTDEGLVATLSLARPESRNALDAALIAEITRCFGE
                     LADDDDVRVVVLTGEGPIFCAGADIGYMRDTAGFSYEENLEDARRLAEMFRSVHELPK
                     PVVAKVRGAAMGGGAGLVAAADVAVSEEGARFAFSEVRLGIAPATIAPFVVRKIGLSQ
                     ARARFLTGERFDAEEAREIGLVHEAVLERDLDAAVDHVVSQLLEGGPEAQAAIKALLH
                     QVEATEPLEALGLMTSLTAELRTSEEGQEGLRAFLEKREPRWRNS"
     gene            complement(1598..3205)
                     /locus_tag="VFT03_10480"
     CDS             complement(1598..3205)
                     /locus_tag="VFT03_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU4848613.1"
                     /translation="MSKIRSHIDPKSEGFRENSDAYEELLLELRERSARARRGGGEKA
                     RKRHEERGKLPVRDRIELLLDPGTAFLELSPLAAFGMYDGRVPAAGIVTGIGRVSGRD
                     VVIVANDATVKGGTYYPITVKKHLRAQEIAEQNRLPCIYLVDSGGAFLPLQDEVFPDR
                     DHFGRIFYNQARMSAKGIPQIAAVMGSCTAGGAYVPAMSDEVVIVKGTGTIFLGGPPL
                     VKAATGEDVTAEELGGADVHTRRSGVADHFAENDEHALAIVRSIMENLNAEKSPPWTV
                     EEPEEPLYDAEDIYGIVPRDYRQGYDVREVIARIVDGSRLHEFKPLYGETLVCGFARI
                     MGMPAGILANNGILFSESALKGSHFIELCASRGIPLVFLQNITGFMVGRAYEAGGIAK
                     DGAKMVMAVANAGVPKFTVIVGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGAQA
                     ANVLLTVQEDNLEREGREMTDEEREELKRPILEKYEEEGNPYHSTARLWDDGVIDPVD
                     TRMVLALGLSAAANAPIEATRFGIFRM"
     gene            complement(3283..3609)
                     /locus_tag="VFT03_10485"
     CDS             complement(3283..3609)
                     /locus_tag="VFT03_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEU4848614.1"
                     /translation="MPLYALNLFDLAENEQYLRYSRRSPGAVAKHGGRVVALGRLDDA
                     LAGDTGPRSVMILVEWPSREAFQAFLDDPELADLHPLREEGTRNYLWWTYERLEDLRP
                     IFPGSA"
     gene            complement(3617..>3783)
                     /locus_tag="VFT03_10490"
     CDS             complement(3617..>3783)
                     /locus_tag="VFT03_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4848615.1"
                     /translation="GGEGFMEGSAVARYWRQVKINEIGEGTSEINRQIIARSLLAESA
                     EAAKANMVAQ"
BASE COUNT          589 a         1365 c         1136 g          693 t
ORIGIN      
        1 gcgttggatc gaacactcgc gctcgtagag gtgtatgacg ttgccctctg cgtccccgat
       61 cacctgcacc tcgacgtgcc tggggtcctc gatgagcttc tccaggaaca ccgacccgtc
      121 gccgaaagct gcctccgcct cacgcctcgc cccccgcacg gcctcggcga agtcctgcgg
      181 gcgcgtgagg gcgcgcatac cgcgcccgcc gccgcccgcg ctcgccttga cgagcaccgg
      241 aaagccgagc gcctcagctt cctcggcgag acgctcctcg gactcttcct ccccatcgta
      301 gccagggacg gttgggacgc cagcctgccg ggccaactcc ttcgccctga ccttgaaccc
      361 catggcttcc atcgcctccg gcggcggccc gacccacaca gcgcccgaag actcgactgc
      421 gcgggcaaag ggcgcgctct cggccaggaa cccgtacccg gggtggactg catccgcacc
      481 tgaggcttct atcgcctcca acagcctctc gatgttcagg tagctctccg cggccggggc
      541 agggcctatg ggatgggctt cgtccgcgag gcggcggtgc atggctttct cgtcagcttc
      601 ggagtagact gcgacgctcc ttatcccgag ctcgcggcag gcgaggatga cgcgcacggc
      661 tatctcgccg cggttcgcga tcaggacctt tccgaagctc acgaattcct ccagcgcggc
      721 tcgcgcttct ccaggaaggc cctcaggccc tcctgtcctt cctcgctcgt tcggagctcc
      781 gctgtaagac tggtcatgag cccgagggcc tccagcggct cggtggcctc gacctggtgc
      841 aacagggcct tgatcgccgc ctgggcctcc ggccccccct ccaacagttg cgagactaca
      901 tgatctaccg cggcgtcgag atcccgctcc agaacagcct cgtggacgag cccaatctcg
      961 cgcgcctcct ccgcgtcgaa cctctctcct gtcaggaacc gcgcccgggc ctgcgagagc
     1021 ccgatcttgc gcaccacgaa cggggctatg gtcgcaggcg ctatcccgag gcgcacctcg
     1081 gagaatgcga acctcgcccc ctcctcggac accgcgacat cagccgccgc cacgagcccg
     1141 gccccgccgc ccatagcagc cccccgcacc ttcgctacca ccggcttcgg gagctcgtgc
     1201 accgaccgga acatctccgc caggcgcctt gcgtcctcca ggttctcctc gtacgagaag
     1261 cccgccgtgt cgcgcatgta cccgatgtcc gcccccgcac agaagatagg gccctcgccg
     1321 gtcagaacca cgaccctcac gtcgtcgtcg tcggcaagct ccccgaagca gcgcgtgatc
     1381 tccgctatca gcgcagcgtc gagcgcgttt cgcgactcgg gacgtgccag cgacagggtg
     1441 gcgacaagtc cctcgtccgt tctccgaaga ttctcgtagg tctcaggcat cagacttcag
     1501 gtcttctgat aacgtaataa gtcgctctgc gtcctgagtc tgcattcgtc cggtcgtgca
     1561 atttcctaac cccttcctga tgcctgaagc ccgaaactta cattctaaag ataccgaagc
     1621 gggtcgcttc gatcggagcg ttcgcggcgg cggagaggcc aagagccaga accatcctgg
     1681 tatccaccgg gtctatgaca ccgtcgtccc agagcctggc ggttgagtgg tacgggttcc
     1741 cttcctcctc gtacttctcg aggatgggcc gcttgagctc ctcgcgttcc tcgtccgtca
     1801 tctctctgcc ctcgcgctcg aggttgtcct cctgcacggt gaggaggacg ttcgcggcct
     1861 gtgctccgcc catgacgctg atgcgggcgt taggccacat ccacaggaag cgcggcgagt
     1921 aggcgcggcc gcacataccg tagttgccgg caccgaagga gccgcccacg atcacggtga
     1981 acttcgggac gccggcgttc gcgacagcca tgaccatctt ggcgccgtcc ttggcaatgc
     2041 cgcccgcctc gtatgctcta ccgaccataa agccggttat gttctgcaag aagacgagcg
     2101 ggatgccgcg tgaagcgcac agctcgataa agtgcgaccc cttgagcgcg ctctcggaga
     2161 acaggatgcc gttgttggcg agtatgccgg ccggcattcc catgatgcgg gcgaagccgc
     2221 acaccagcgt ctcgccgtaa agcggcttga actcgtgcag cctgctcccg tccacgatcc
     2281 gggcgatgac ctcccgtaca tcgtagccct ggcggtagtc ccgcggcacg atgccgtaga
     2341 tgtcttctgc gtcgtagagc ggttcttcgg gctcctccac ggtccacggc gggctcttct
     2401 ccgcgttcag gttctccatg atggagcgta ctatggccag ggcgtgctcg tcgttctcgg
     2461 cgaagtggtc ggctaccccc gagcggcggg tatggacgtc ggcgcccccg agctcctcgg
     2521 cggtcacatc ttcgccggtc gcggccttga cgagaggtgg accgccgagg aatatcgtac
     2581 ccgtaccctt cacgatcacg acctcgtcgc tcatcgccgg cacgtaggcc ccgccagccg
     2641 tgcagctccc catgacggcg gctatctgag gaattccctt cgcgctcatc ctcgcctggt
     2701 tgtagaagat gcgcccgaag tggtcccgat ccgggaacac ttcgtcctgc aacggcagga
     2761 acgctccccc cgaatccacc aggtagatac acggaagacg gttttgctcg gcgatctcct
     2821 gtgcgcgaag gtgcttcttg accgttatgg gatagtacgt gccacccttt accgtcgcat
     2881 cgttggcgac gatcacgaca tctcgccccg agacgcgtcc gatgccggtg acgatgcctg
     2941 ccgccggcac cctcccgtcg tacatcccga acgcggctag aggcgagagc tcgagaaagg
     3001 cggtgccggg gtcgagcagc aactctatgc ggtcgcgcac cggcagcttc ccgcgctcct
     3061 cgtggcgctt ccgcgccttc tcgccgcctc cgcgccgcgc ccgggcggag cgctcgcgca
     3121 actcgaggag aagctcctcg taggcgtcgc tgttctccct gaacccctcg ctcttcgggt
     3181 ctatgtggct gcggatcttg ctcaaaccct ctcccgctct gtcataggtg atgttatccg
     3241 gcgcgagtta caggcgccac cggggagcgc agatcctggt actcaggcgg agccggggaa
     3301 gatcgggcgc aggtcctcca ggcgctcgta ggtccaccac aggtagttac gcgtcccctc
     3361 ctcccgcagc gggtgcaggt ccgcgagctc cgggtcgtcg aggaaggcct ggaacgcctc
     3421 gcgcgagggc cactcgacca ggatcattac gctgcggggc ccggtgtcgc ccgccagggc
     3481 gtcgtccagc cgcccgagcg cgacgacgcg cccgccgtgc ttcgccacgg ccccgggcga
     3541 gcggcgcgag tagcgaagat actgctcgtt ctcagccagg tcgaagaggt tgagggcgta
     3601 gagcggcatc gtcaggttac tgcgccacca tattcgcctt cgccgcttcg gcgctctcgg
     3661 cgagcaggct gcgggctatg atctggcggt ttatctcgct ggtcccctcg ccgatctcgt
     3721 tgatcttgac ctgccgccag tagcgcgcca ccgcgctccc ctccatgaac ccctcgccgc
     3781 cgt
//
LOCUS       DASTUV010000518         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_778095, whole genome shotgun sequence.
ACCESSION   DASTUV010000518 DASTUV010000000
VERSION     DASTUV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778095"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /gene="glmM"
                     /locus_tag="VFT03_10495"
     CDS             <1..1379
                     /gene="glmM"
                     /locus_tag="VFT03_10495"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU4848616.1"
                     /translation="FFGRLERGPSRAEEVYLQRRPITFGTDGARGVANEGLIPEDALR
                     LGLAAARLFGGPILICRDTRLSGGMLSSALAAGAASGGARVIDAGVLPTPGVAALASR
                     LGAAAAGVVSASHNPYPDNGVKFFCGEGQKLSGAKEREIEALAADTDPERPARSGIGP
                     VEQLDGAARLYVEGVLERLRPRVGGMKVLLDCANGAAYEAAPLAFEELDVDLTVVGAK
                     PDGTNVNANCGSTHIQRLDASGHDVAFALDGDADRVLAKDEKGEVVDGDRIMAILARD
                     LQERPALEGGVVVTVMSNLGFFKAMESMNIAYEVTPVGDRHVAEAMRARRVVLGGEQS
                     GHVILSEHVTTGDGLVTALALLDVMARSGRPLSELAEVMEAYPQQLINVAVADAASAK
                     SLAASAAVGRAVSEAEEQLGEEGRILLRPSGTEPVVRVMVEHADEDVCREVCEEVARV
                     VGEAEVRA"
     gene            1376..2257
                     /locus_tag="VFT03_10500"
     CDS             1376..2257
                     /locus_tag="VFT03_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848617.1"
                     /translation="MRFVGCALAWRPGEARDRASCLVVLDERGSIIANSFAADAGEIA
                     AAIEDYVAERRGVVVGADAPLAVPNERGTRRIERILSKVALPAYSASRKMFGGEPYPE
                     ELLAQLEGVGIEYTDYPFPRTRGQRVVTEVDSAAALKILALERTARDGEPSWNQPNGR
                     DLAQVLRGMEELKLRKGNKEARASALKQAVDILWNTPGLRLRTGNLSGDISALENIDL
                     SRIDVNAGLSHAELDRIASLLEGTLAAYTVHRHWRGRDGSMVVGTGDEGSVLLPTPEA
                     LHARIAEESRAAGVPYV"
     gene            2313..>2761
                     /locus_tag="VFT03_10505"
     CDS             2313..>2761
                     /locus_tag="VFT03_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU4848618.1"
                     /translation="MKGTKFENLRDAGDPVELAALYDREGADEIVFYDITASHEGRNT
                     ATQLARRTAEEVFVPYTIGGGVRTADDMRAMLRAGADKVSINSAAVRNPDLINEGAER
                     FGSQCVVLSVDVRRRASAPGWEVYLNGGRVNTGIDALSWLVEGERRGA"
BASE COUNT          462 a          852 c         1032 g          415 t
ORIGIN      
        1 gcttcttcgg gcggctcgag aggggcccat ctcgcgccga ggaggtctat ttgcaaagaa
       61 gaccgatcac tttcggcacc gatggggcgc gtggggtcgc gaacgagggc cttatccccg
      121 aggacgcgct gaggcttggc ctcgccgccg caaggctctt cgggggccct atcctgatct
      181 gccgcgacac ccgcctctct ggggggatgc tttcttccgc cctcgctgca ggcgcggcga
      241 gcggcggggc gcgggtgatc gacgcagggg ttctgcctac ccccggcgtc gccgccctcg
      301 cgtccaggct cggagcggcc gcagcgggcg tggtgagcgc ctcgcacaac ccctacccgg
      361 acaacggcgt aaagttcttc tgcggcgagg gccagaagct ctcgggagcg aaggagcggg
      421 agatcgaagc cctcgccgca gacacagacc ccgagcgccc cgcgcgaagc ggtatcggcc
      481 ctgtcgagca actcgacggg gctgcgaggc tgtacgtcga gggcgtcctg gagcgtctgc
      541 gcccgcgcgt cggggggatg aaggtgctgc tcgactgcgc gaacggggcg gcgtacgagg
      601 ccgcgccgtt ggccttcgag gagttggacg tagaccttac ggtcgtcgga gccaagcccg
      661 acggtacgaa cgtaaacgcc aattgcggct cgacgcacat acaacgtctc gacgcctcgg
      721 ggcacgacgt cgctttcgcc ttggacgggg atgcggacag ggtgctcgcc aaagacgaga
      781 agggcgaggt cgtggacggg gacaggatca tggccatcct cgccagagat ctccaggagc
      841 gccccgcgct cgagggcggg gtggtagtaa ccgtgatgag caacctcggc ttcttcaagg
      901 cgatggagtc catgaacatc gcatacgagg tgacccctgt cggcgacagg cacgtggctg
      961 aggctatgcg cgcgcgccgg gtggtcctcg ggggtgagca gtcgggacac gtgatcctct
     1021 cggagcacgt cactacaggg gacggcctgg taaccgcgct ggcgctcctc gacgtgatgg
     1081 cccggagtgg caggccgctc tcggagctgg ctgaggtcat ggaggcctat ccccagcagc
     1141 tcatcaatgt tgccgttgcc gacgcggcct ccgcgaagtc cctcgccgct tcggcggcgg
     1201 tggggcgggc cgtctcggaa gctgaggagc agctcggcga ggaggggcgc atactgttga
     1261 ggccgagcgg aacggagccc gtggtgcggg tcatggtcga gcacgcagac gaggacgtgt
     1321 gccgcgaggt ctgcgaggag gtcgcgcggg tggttggaga ggcggaggtc cgggcgtgag
     1381 gttcgtgggt tgcgcgctgg cgtggaggcc gggcgaggcg cgagatcgcg cttcgtgcct
     1441 ggtggtgttg gacgagcggg ggagcataat cgccaactcc ttcgcggcgg acgcggggga
     1501 gatagctgcg gcgatcgagg actacgtcgc cgagcgccgg ggcgtggtcg ttggtgctga
     1561 cgccccgctc gccgtcccga acgagagggg gacgcgcagg atcgagcgca tcctctccaa
     1621 ggttgccctc ccggcctact cggcgagccg caagatgttc ggcggggagc cgtaccccga
     1681 ggagctcctg gcccagctgg agggagtcgg gatcgagtac acggactacc cattcccgag
     1741 gacgcggggc cagagggtag tcaccgaggt cgactcggcg gccgcgctca agatcctggc
     1801 tctcgagcgc accgcccgcg acggcgagcc ttcgtggaac cagccgaacg gcagagacct
     1861 cgcgcaggtg ctgcggggga tggaggagct gaagctgagg aagggcaaca aggaggcgcg
     1921 ggcctcggcg ctcaagcagg cggtggatat cctgtggaac acccccggtc tgcgcctgag
     1981 gacgggaaac ctctcggggg atatctccgc cctcgagaac attgatctct cgaggatcga
     2041 cgtgaacgcc gggctatctc acgcggagct cgacaggatc gcgagcctcc tcgaagggac
     2101 gctcgctgcc tacacggtgc accggcactg gaggggaagg gacggctcga tggttgtcgg
     2161 aaccggagac gagggctccg tcttgctgcc cacaccggag gcgctgcacg cccgcatagc
     2221 cgaagagtca agggccgccg gggtgccgta cgtctagcat gggtctccta gtacgcgtaa
     2281 tcccctgtct cgacgtagac gcaggccgcg tcgtgaaggg gacgaagttc gaaaacttgc
     2341 gggacgcagg cgaccccgtc gagctcgccg ctctctacga ccgcgagggg gctgacgaga
     2401 tagtcttcta cgacatcacc gcctcccacg aggggagaaa cacggccacg cagctcgccc
     2461 gccggaccgc agaggaggtc ttcgtccctt acaccatcgg cggcggggtg aggaccgctg
     2521 acgatatgcg cgccatgctg cgcgccggag ccgacaaggt ctccataaac agcgccgccg
     2581 ttcgcaaccc cgacctcata aacgagggcg cggagcgctt cgggagccag tgcgtcgtcc
     2641 tcagcgtgga cgtcaggcgc cgcgcgtccg cccctggctg ggaggtctat ttgaacggtg
     2701 ggcgggtcaa cacggggata gatgctctct cgtggctcgt cgagggggag aggcgggggg
     2761 c
//
LOCUS       DASTUV010000519         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1589804, whole genome shotgun sequence.
ACCESSION   DASTUV010000519 DASTUV010000000
VERSION     DASTUV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589804"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..>2226)
                     /gene="dnaE"
                     /locus_tag="VFT03_10510"
     CDS             complement(62..>2226)
                     /gene="dnaE"
                     /locus_tag="VFT03_10510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4848619.1"
                     /translation="YSDTDKLAKFIPDKPVGTTLRDVLKPGEDGYVAGEKHPGAARDI
                     IQYVEKDEAAKQVLDTAFEIEGFARHAGTHAAGVVISEEPLTDIVPLQLIRARGASET
                     EEELPVMVQHPMSDVEALGLLKVDFLGLRNLDVIEATLETIKETTGEELDIRTIPLDD
                     EKTLRLFARGDTFGVFQFESSGMQRMLQEVRPDRFDDLVALNALYRPGPMDYIPNFKR
                     GKHDPESVKYLDPRLKPILEPTYGVAAYQEQLMEISKSLGGFTPGEADTLRKAIGKKN
                     VRLLAPLKDKFTSGCEQNRVAPEVAEELWNWMEKAGGYSFNKSHSACYSFLAFQTAYL
                     KAHYPEAYMAALMSSVMNTKDRVPQYVAEARAMKIRVLPPDVNESGRRFTVVGVTIRF
                     GLSAVKNVGDSCVEAIIAAREEGGPFEDLFDFCERVESSTFNKRTLESLIKCGAFDGT
                     GHPRAAMLAVHGQAVERAAKRKTSAGDDQFCMFDSTELANLAPPKPEIPAIEDDRRRS
                     LEWEKETLGLFVSDHPLRPVLHKLKKHADTTVSDLDGCRDGAVVWVGGLATSVRVNTT
                     RKGDQMAMLQLDDTRGLAEVIVFPRVYASCADCIREDAVLKVKGRVEHKEGIPRIVAL
                     EMEELHLEPGLDPVYLHAACFVDLPRSAARQAFEIIGRHQGSSPLFLVSSDGELEERL
                     GGVEDSSDLHAELKQLLGPKCIGYLRPQSTEPEMEQVS"
BASE COUNT          358 a          727 c          693 g          448 t
ORIGIN      
        1 taggggtaga tcaactgtta tggggaatcg ccgcgaagga gccgttcccc ggccggcggc
       61 ttcaggagac ctgctccatc tccggctcgg tggactgcgg ccttaagtag cctatacact
      121 tcggcccgag caactgcttg agctctgcgt gcaggtccga tgaatcttcg acgcccccga
      181 gccgctcttc caactctcca tcgctcgaga cgaggaatag cgggctgctg ccctggtgcc
      241 ggccgatgat ctcgaacgcc tgccgcgcgg cggagcgcgg cagatccacg aagcacgccg
      301 cgtgaaggta cacgggatcc aggcccggct cgaggtgtag ctcctccatc tcgagcgcga
      361 cgatgcgggg tatgccttcc ttgtgctcga cgcggccctt taccttgagc acggcgtctt
      421 ccctgatgca gtccgcgcag ctcgcgtaga ccctggggaa gacgatgacc tcggcgagcc
      481 cgcgcgtatc gtcgagctgg agcatcgcca tttgatcgcc cttgcgcgtc gtgttgaccc
      541 gcaccgaggt tgcgagcccc ccgacccaca caacagcccc gtcccggcag ccgtcgaggt
      601 ccgagacggt cgtgtcggcg tgcttcttga gcttgtgcag cacaggtctc aaaggatggt
      661 ccgagacgaa gagcccaagg gtctccttct cccactcgag gctccggcgc cggtcgtctt
      721 ctattgccgg gatctccggc ttcgggggcg ccaggttcgc cagctcggtc gagtcgaaca
      781 tgcagaactg gtcgtcgcct gcgctcgtct tgcgcttcgc tgccctctcg accgcctggc
      841 cgtgaacggc gagcatggcg gcgcgcgggt ggcccgtgcc gtcgaaggcg ccacacttta
      901 tgagcgactc gagggtgcgc ttgttgaagg tgctcgactc cacgcgctcg cagaagtcga
      961 agaggtcctc gaagggaccg ccctcctccc gcgcggcgat gatcgcctcg acacaagagt
     1021 ccccgacgtt cttgacggcg gataggccga agcggatggt cacgccaaca accgtgaagc
     1081 gacgcccgct ttcgttgacg tcgggcggca ggaccctgat cttcatcgcc cgcgcctccg
     1141 cgacgtactg gggcaccctg tctttggtgt tcatgacgct gctcatcaaa gccgccatgt
     1201 acgcctccgg gtagtgcgcc ttcagatatg ccgtttgaaa ggccaggaac gagtagcatg
     1261 ccgagtggct cttgttgaaa gagtagccac ccgccttctc catccagttc cacagctcct
     1321 cggcgacctc gggcgcgacc cggttctgct cacacccaga ggtgaacttg tccttgagcg
     1381 gcgcgagcaa cctgacgttc ttcttcccga tggccttgcg aagggtatcc gcctcgcccg
     1441 gcgtaaagcc gcccagagac ttggagatct ccatgagctg ctcctggtaa gcagcgacgc
     1501 cgtacgtcgg ctccaggatg ggcttgaggc ggggatccag atacttcacg ctctcggggt
     1561 cgtgcttgcc gcgcttgaag ttggggatgt agtccatggg tccgggacga tacagtgcat
     1621 tcagggccac gaggtcgtcg aagcggtcgg gcctgacctc ctggagcatc cgctgcatcc
     1681 cgctcgactc gaactggaag accccgaagg tgtcgcccct ggcgaatagc ctgagcgtct
     1741 tctcgtcgtc aagcgggatc gtgcgtatgt ccaattcctc gccggttgtc tccttgatcg
     1801 tctccagcgt cgcttctatg acgtcgaggt tcctgagtcc caggaagtcc accttcagga
     1861 gcccgagagc ctcgacgtct gacatggggt gctggaccat gacaggcagt tcctcttcgg
     1921 tttccgaggc ccctctggcc ctgataagtt gaagtggaac gatgtccgtc agaggctcct
     1981 ccgagatgac gacgcctgcc gcgtgagtcc cggcgtggcg ggcgaagccc tctatctcga
     2041 aggcggtgtc gaggacctgc ttcgccgcct cgtccttctc cacgtactgg atgatgtcgc
     2101 gcgccgctcc agggtgcttc tctcccgcta cgtacccgtc ctcgccgggc ttcagcacat
     2161 cgcgcagcgt cgtgccgacc ggcttatcgg ggatgaactt ggcgagctta tcggtgtcgg
     2221 agtatg
//
LOCUS       DASTUV010000520         3977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_517159, whole genome shotgun sequence.
ACCESSION   DASTUV010000520 DASTUV010000000
VERSION     DASTUV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517159"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFT03_10515"
     CDS             complement(<1..855)
                     /locus_tag="VFT03_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4848620.1"
                     /translation="MAGEVRYYSVEEAARVLRLTPERVLEMLEAGELDGIPTGRATWR
                     ITIDGDPDVPPPVTAVPTEPPTGDSEAREVPEEPGEPGVPPATLEEARQPAELFHGDH
                     VATNREPTSESGWVSTQQAAKALGISARTVRWHIERGNLEAIPEGEGVERTWRVSVDS
                     LQAYRDSRQAAAPSPRGNRASEIGSDIAAESPGNAIRELADRLVEEARRAEAARVRLE
                     LSERAQSTLEAELADERRRREEAERTAEDLRRELDEWRRLEESAETTAHEIHDRRGEE
                     RVAQLEEEI"
     gene            complement(921..1187)
                     /locus_tag="VFT03_10520"
     CDS             complement(921..1187)
                     /locus_tag="VFT03_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848621.1"
                     /translation="MGTTLQRRVARLEDASGGDEGCPRCVGAAVIIKDHRGGFIRASW
                     NGEEITKEELRERQAERECPRCSRKIDPDEATQIKVGGTWGSIL"
     gene            complement(1160..1456)
                     /locus_tag="VFT03_10525"
     CDS             complement(1160..1456)
                     /locus_tag="VFT03_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848622.1"
                     /translation="MVTSVAAKLARLEEATGGGGWDAPPCDECGWGGGEEPLDHHNGD
                     TYEIVVDKRPYDEPAEEETCSTCGRVLSMTIFFDDLADHERRERRGWGPPYRGG"
     gene            complement(1932..2144)
                     /locus_tag="VFT03_10530"
     CDS             complement(1932..2144)
                     /locus_tag="VFT03_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848623.1"
                     /translation="MSEDLEHLTDEDYEAIGAIVEGSSRRRQGEDVLAAVEASDAARQ
                     GPDPLLALLERAHQARAEPPDNAACV"
     gene            complement(2141..2740)
                     /locus_tag="VFT03_10535"
     CDS             complement(2141..2740)
                     /locus_tag="VFT03_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848624.1"
                     /translation="MTRTTVTGVRGVPLPNLEAHGLGRKMKEAKQLIDEQARLRQRVS
                     ELGAEQQQLREEIKRRKHEHTQEWGRAMRTGEEAPSGEDIRRAEKRLEAVTSEIGAVR
                     HAGDLADAELRQTVAEHREEWDRAVGERAEAILGEAQELATALAAKLSETEGLVGVHS
                     WLRSSGQSYTPASPAAVSIDHLLHERRRELGLLEAGVVG"
     gene            complement(2880..3059)
                     /locus_tag="VFT03_10540"
     CDS             complement(2880..3059)
                     /locus_tag="VFT03_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848625.1"
                     /translation="MSQLRTGPLFWILLVFAAGFARYFFLAMRDGDDELAIAAFVAYL
                     LVCVVTVAELFGLVW"
     gene            complement(3056..3376)
                     /locus_tag="VFT03_10545"
     CDS             complement(3056..3376)
                     /locus_tag="VFT03_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848626.1"
                     /translation="MTSHGQAIRRRRVSRRVPRVGVAAGVAVGFAMGVVTEVLLMGGG
                     WYSVLEEILYPLAFVFALVGAFSGGFLLLAAEHDGNQRRAWRAAVVFATSSVFAAVLF
                     WGVP"
     gene            complement(3619..>3977)
                     /locus_tag="VFT03_10550"
     CDS             complement(3619..>3977)
                     /locus_tag="VFT03_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848627.1"
                     /translation="VEPDDVLKAVALSDYFKDQARRVFGALRGYDEKKRLLEDVSRFV
                     AKEGGVWIGTATELHQQFQSDYKPQRAEELSKFIQQAAKDEEGFTYDSATVRYKDESS
                     GEWKSRREITLYRQTP"
BASE COUNT          633 a         1403 c         1094 g          847 t
ORIGIN      
        1 gatctcctcc tcgagctgtg cgacccgctc ctctccccgc cgatcgtgta tctcgtgcgc
       61 ggtcgtctcg gccgactctt ctagccgacg ccactcgtct agctcacggc ggagatcctc
      121 ggcggtccgt tcagcctcct cgcgccggcg ccgttcgtcg gccagctccg cttcgagggt
      181 ggactgggcc ctctctgaga gctccaggcg cactcttgct gcttccgccc gcctcgcctc
      241 ctccacaagc ctgtccgcaa gctcccggat agcattgccg ggactctctg cggcaatgtc
      301 cgagccgatt tcggaggcgc ggttgccgcg cggtgaaggc gcggcagctt gccgcgaatc
      361 cctgtaggcc tggaggctgt ctacggaaac tctccacgtg cgctccaccc cttcaccttc
      421 gggtatagcc tcaaggttac ctcgctcgat atgccagcgg acagtccttg cggatatccc
      481 tagagccttt gccgcctgtt gcgtgctcac ccatcctgat tcggaggtgg gctcgcggtt
      541 agttgccaca tggtctccgt ggaagagttc ggccggttgc cgcgcctcct cgagggttgc
      601 tgggggcact ccgggctctc ctggctcctc aggaacttct ctggcctcgc tgtcgccggt
      661 agggggttcg gttggtactg ccgtaacggg cggcggcacg tcagggtcgc cgtctatggt
      721 aatcctccac gtagctcggc cggtcgggat gccatcgagc tctccagcct cgagcatctc
      781 caggacacgc tcgggcgtaa gccgcagtac tcgtgcggcc tcctccacgg agtagtaacg
      841 gacctctccg gccacagctc acacactacc ctagtccggg acctagcgca cctcgagggg
      901 cttagaagcc tgtcatgctg ttacaagata gacccccagg taccaccgac cttgatctgg
      961 gtggcctcgt cggggtctat ctttctgctg catctaggac actcgcgctc cgcctggcgc
     1021 tcacggagct cctctttggt tatctcctcg ccgttccagc tagctcgtat gaagccgccg
     1081 cgatggtcct tgatgataac cgcagctccc acgcatctag gacagccctc atcgccgcca
     1141 cttgcgtcct cgagccgcgc taccctcctc tgtaaggtgg tccccatcct ctgcgctccc
     1201 ttcgctcgtg atctgccagg tcgtcgaaga agatggtcat ggagagaacc cggccacagg
     1261 tcgagcaggt ctcttcctct gccggctcat catacggacg cttatcgacg actatctcgt
     1321 aggtgtcgcc gttgtggtgg tcgagaggct cctcaccacc accccagccg cactcatcac
     1381 acggtggggc gtcccatcca cccccgcccg tggcctcctc cagccgcgct aactttgccg
     1441 ctacgctggt caccatgcct tcctacctct ctggttgggg tttcgttggt tgacgctcag
     1501 gcgcctctct aattcttcta cgcgctcgta aagttcctcc tgctcacgtg ctttaagaag
     1561 cccgagcacg cacgccctag cgccgtttag cagctgcgcc gcgacggcac catcgccacg
     1621 ctctacctca cccgaaagga ccttactcac cagctcctca aagcgttcct gcagacggac
     1681 gagttcacct gtggccggcg atgggcgccc tcgaccgcca cgcttgccgc ccttgtgggc
     1741 cgcccgcctt ctgtcgtcag cgtggttggg atgatgggcg tagcagtagc ccgagctatc
     1801 aatagctata cccttacagc ggttgccgcc ctgggtaatt gcggtacatt tagccaatag
     1861 ctacctaccc tccccctccc cttcggcggt gaggaatacc ttgcggttct cccagaacga
     1921 gagcaacgca cttagacgca tgccgcgttg tcgggcggct ccgcccttgc ctggtgtgcc
     1981 cgctccagca acgcgaggag cgggtcaggt ccttggcggg ccgcgtcaga ggcttccacg
     2041 gcggcgagaa catcttcgcc ctgtcgccgg cggctcgagc cctcgacgat ggcgcctatg
     2101 gcctcgtaat cctcgtcggt aaggtgctcg aggtcctcgc tcatccaacc acccccgctt
     2161 caagcaaccc gagctctcgc cggcgctcat ggaggagatg gtcgatactg accgccgccg
     2221 gcgacgccgg cgtgtaggac tgtccagagc tccgcagcca cgaatggacc ccgacgaggc
     2281 cttcggtctc tgagagcttg gctgcgagag cggtggcgag ctcctgcgcc tcgccgagga
     2341 ttgcctctgc cctctcccct actgctcggt cccactcttc acggtgttcg gccacggtct
     2401 gcctcagctc ggcgtcggcg aggtccccgg catgacgtac ggcgccgatc tcactcgtaa
     2461 cggcctcgag gcgcttctca gcccgccgta tatcctcccc tgagggcgct tcctcgccgg
     2521 tacgcatggc acgcccccat tcctgtgtgt gctcgtgctt gcgccgttta atctcctccc
     2581 ttagctgctg ctgttcggcg ccgagttcgc tgacccgctg acgaaggcgg gcctgctcat
     2641 ctatgagctg cttggcttct ttcatcttgc gccccaggcc gtgagcctcg aggttcggga
     2701 gtggcacgcc ccgcactccc gttactgtgg ttctggtcat acttctcctt tcgtttgctc
     2761 tgtctctagt agcttctctg ccttctccct atgctcctcc gctagtcggc tcacccgtgg
     2821 ccgaacattc gtttgatcgt cggcgtaaaa aaagagaaag cctgccgacg ggatcctact
     2881 caccacacca acccaaacaa ctccgccacg gtcaccacgc acaccagtaa gtaggccacg
     2941 aacgcggcga ttgccagctc gtcgtcaccg tctctcatgg cgaggaaaaa gtagcgggca
     3001 aaccccgcag cgaataccag gaggatccag aacaatggcc ccgtgcgcag ctggctcacg
     3061 gcacgcccca gaaaaggacc gcagcaaaca ccgaggatgt tgcgaaaacc acggcagcgc
     3121 gccaggcgcg gcgctgattc ccgtcatgct ctgccgcaag cagtaggaac ccgccggaga
     3181 acgcacccac cagcgcaaac acgaacgcga gcgggtacag gatctcctcg agaactgagt
     3241 accagccccc gcccatcaag aggacctcgg tgaccactcc catcgcgaac cccaccgcta
     3301 ctccggctgc cacgccgacc ctcggaaccc gtctgctgac gcgccgacgc ctgatggctt
     3361 gtccatgtga cgtcatgccg ctcttctccc ctcgccccta gccgccctgc agagctccgc
     3421 cagagcctcg agataggcgg gccagttgcc ggtcctctcc gccttgtatg cggccgctgt
     3481 gtggatgtct agcgatgagt aatccgtgcc cgggggtagg gtccgcgaga cgtagcggta
     3541 agcctgctcc gccctccgtg cagctctctc agcatcccat gtagtcatcg tgtctcatct
     3601 cgttacggcg ttacggcgtt acggcgtttg cctatagagt gtgatttcac gcctgctctt
     3661 ccactcgcca ctcgactcat ccttgtatcg gaccgttgct gagtcgtagg taaagccttc
     3721 ttcgtctttt gcagcctgct gaatgaactt ggatagctcc tctgccctct gtggtttgta
     3781 gtcgctttga aactgctgat gtagctctgt tgccgtacca atccacaccc cgccctcttt
     3841 ggcgacgaac cgagagacgt cctcaagtag cctcttcttc tcgtcgtaac cccgcagagc
     3901 accgaagacg cggcgggcct ggtctttgaa atagtcgctg agtgcaacgg cctttagaac
     3961 atcgtctggc tcgactc
//
LOCUS       DASTUV010000521         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1110008, whole genome shotgun sequence.
ACCESSION   DASTUV010000521 DASTUV010000000
VERSION     DASTUV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110008"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFT03_10555"
     CDS             <1..813
                     /locus_tag="VFT03_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU4848628.1"
                     /translation="LGNGLLSLMLAMVLMPFLEDAFNILTPMKLLELSDPRNPLLDKL
                     LRNAPGTFSHSMQVGLLAERAAERVGADALLAQVGAYYHDIGKMEHPAYFVENQIAQI
                     NPHAALSPTLSAKVIKRHVKDGLEIGTAWGLPQEILDIIAQHHGSTRIEYFYRKALED
                     APDEEAVRESDFRYSSGQPRSKEAGIMMLADSIEATVKSLPKPTPKRIDDVVADTIRR
                     KIQDGQFDKCQLTMHDIHEAGEAIRESLIGFLGPRIEYPEDPGHARNGKAAS"
     gene            881..1267
                     /gene="ybeY"
                     /locus_tag="VFT03_10560"
     CDS             881..1267
                     /gene="ybeY"
                     /locus_tag="VFT03_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4848629.1"
                     /translation="MDYAELDTERATQLFAASFSNLGLDLARIGEVSVVLVSPETIRD
                     LNSRFRDRDEPTDVLSFEVDGPYGEMVGEVVICPVCADTERGVDELVVHGALHLGGMD
                     HGEDFGASEMARVQRAVMEELRGEES"
     gene            1251..1994
                     /locus_tag="VFT03_10565"
     CDS             1251..1994
                     /locus_tag="VFT03_10565"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU4848630.1"
                     /translation="MAKKAERPSKGQQGVARSFEHAYRGMISAVRTQRNMRFHVVVAV
                     VVLVASLLVGVSKVELAVLILTILLVFVTELFNTAMEFVVDLATREYHPLAKLVKDVS
                     AGAVLVSSVGALLVGYLVLADDLGPLSLATLESIRRQPAHLTLVTLGVIVLIVLLGKA
                     LTHSPNSFAGGMPSGHAAVAFAGWVAASFIAVQGGAGSYAGLISVISLLMALLVCQSR
                     VESGLHSVYQIVMGAVIGVLVTTAAFQFL"
     gene            2006..2398
                     /locus_tag="VFT03_10570"
     CDS             2006..2398
                     /locus_tag="VFT03_10570"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU4848631.1"
                     /translation="MDPEAVMEAARAAAKRAYAPYSNFHVGAAILTGGSTIYSGANVE
                     NASYGLSVCAERNAVAMMATTDPDDREIELVAVFSPNTSPCFPCGACRQVLREFGCKE
                     VLVETGRGLQRYPFDQVLPNSFGPEELG"
BASE COUNT          459 a          763 c          739 g          453 t
ORIGIN      
        1 ctcgggaacg gcctcctctc gctgatgctg gcgatggtgc tgatgccttt cctggaagac
       61 gccttcaaca tcctcacgcc gatgaagctg ctcgagcttt ccgacccccg aaatcccctg
      121 ctcgacaagc tcctgcgcaa cgctcctggt actttctccc actcgatgca ggtagggctc
      181 ctcgctgaga gggctgcaga gagggtcggc gctgacgcgc tactcgccca ggtcggagcc
      241 tactaccacg atatcggcaa gatggaacat cctgcctact tcgtcgagaa ccagatcgcc
      301 cagatcaacc ctcacgccgc cctctccccg acgctctcgg ccaaggtgat caagcgccac
      361 gtcaaggacg ggctcgagat aggcaccgcc tggggattgc cgcaagagat cctggacata
      421 atcgcccagc atcacggatc gacccgcatc gagtacttct accgcaaagc cctcgaggac
      481 gcccccgacg aagaggcggt ccgcgagtcg gatttccgct actcaagcgg ccagccacgt
      541 agcaaggagg caggcatcat gatgctcgcc gactcgatag aggcgaccgt gaagtctctg
      601 ccaaagccga cccctaagcg tatcgacgat gtcgttgccg atacgatcag gaggaaaatt
      661 caggacggcc agttcgacaa gtgccaactc accatgcacg atatccacga agcaggggag
      721 gccatccgcg agtcgctcat aggtttcctc gggccgcgca tcgagtaccc cgaagaccca
      781 ggccatgccc gcaacggaaa ggcggcgagt taggccatac catacacgga gataccatct
      841 gaggggtgat ccacccatac ccgtcgccgt tctggatgag gtggactatg ccgagctcga
      901 caccgaacga gccacccagc tcttcgccgc ttctttctcc aacctgggtc tcgacctggc
      961 gcgaatcggc gaggtctctg ttgtactcgt cagccccgag acgatccgtg acctcaactc
     1021 gaggttcaga gatagggacg agcctaccga tgttctcagc ttcgaggtgg acggacctta
     1081 cggggagatg gtaggcgagg tcgtgatctg tcccgtctgt gcggacactg agaggggcgt
     1141 cgacgagctc gtggtgcacg gcgcgctaca cttgggtggc atggaccacg gtgaggactt
     1201 cggggctagt gagatggcga gggtccagcg ggccgtaatg gaggagctgc gtggcgaaga
     1261 aagctgaacg gccaagcaag ggccagcagg gcgtagcccg cagcttcgag cacgcctacc
     1321 ggggtatgat ctccgcggtc aggacccagc gcaacatgcg cttccacgtg gtagtggccg
     1381 tcgttgttct cgtagcgagc ctgctggtcg gagtcagcaa ggtggagctc gcggtcctta
     1441 ttctgaccat actcctggtc ttcgtgaccg agctgttcaa cacggccatg gagttcgtag
     1501 tcgaccttgc cacaagggaa taccatcctc tggccaagct agtcaaggac gtctcggcgg
     1561 gggcggtcct ggtctccagc gtcggggctc tgctcgtcgg ttatctcgtc ctggcggacg
     1621 atctggggcc gctctccctg gcgactctgg agagcataag gcgccagccg gcccacctca
     1681 cgctcgtcac gctcggtgtg atcgtactca tcgtcctcct cggcaaggcg ttgacccact
     1741 ccccgaactc cttcgccggc gggatgccct ccgggcacgc ggcggtcgcc tttgcaggat
     1801 gggtcgccgc gagcttcata gccgtccagg gtggcgcggg gtcctacgcc gggctcatct
     1861 ccgtgatctc gctcctcatg gcactgctcg tctgccagag ccgcgtcgag agcgggcttc
     1921 acagcgtcta ccagatcgta atgggggccg tgatcggggt cctcgttacg acggcagcct
     1981 ttcaattctt gtgaaaggag gagccttgga ccctgaggcc gtgatggagg ccgcacgtgc
     2041 cgctgcaaag cgggcatacg caccatacag caacttccac gtcggcgcgg ccatcctcac
     2101 aggaggtagt accatatact caggcgcgaa cgtcgagaac gcttcttacg ggctctcggt
     2161 ctgcgcggag cgcaacgcgg ttgcaatgat ggccactaca gatcctgatg acagggagat
     2221 cgaactcgtc gccgtcttca gcccgaacac ctcgccgtgt ttcccttgcg gagcctgccg
     2281 gcaggtcttg cgggagttcg gctgcaaaga ggtcctcgtg gagaccggtc gtgggctaca
     2341 gcgctacccc ttcgaccagg ttcttcccaa ctccttcggt ccggaggagt tgggatgaag
     2401 aacaacgggt tccg
//
LOCUS       DASTUV010000522         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_911396, whole genome shotgun sequence.
ACCESSION   DASTUV010000522 DASTUV010000000
VERSION     DASTUV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911396"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1262
                     /gene="lipA"
                     /locus_tag="VFT03_10575"
     CDS             360..1262
                     /gene="lipA"
                     /locus_tag="VFT03_10575"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU4848632.1"
                     /translation="MPFRQQRGLHGRPEWLKARAPVGEGYTDIKETMRGLNLHTVCEE
                     ARCPNIGECWNNRTATFMILGNVCTRSCGFCAVLTGKPTELDLDEPRRVADAAGQMGL
                     KHAVITSVNRDELADGGAAVFAATIREIRTHVPGCAVEVLTPDFKGDRDAIKTVIDAR
                     PDTFNHNIETVPRLYPAVRPQAKYQRSLEVLRYAKELNSEGLTKSGFMVGLGEVEEEI
                     QRTMLDLREHDVDILTIGQYLRPSENHLPMSRYYAPKEFAAFRKLGFEMGFKHVESGP
                     LVRSSYHAHEQTEDARSSALGASV"
     gene            1274..1933
                     /gene="lipB"
                     /locus_tag="VFT03_10580"
     CDS             1274..1933
                     /gene="lipB"
                     /locus_tag="VFT03_10580"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU4848633.1"
                     /translation="MALEKQAPASFIEVRRLPGLVPYAEAWELQAELVQLRKADSVPD
                     TLLLLEHPPVFTVGRAAKDASNLGAGEGYLRSLGAEVFWSDRGGDATFHGPGQLVGYP
                     ILRLRDRDTHGYLRRLEAIIIRALADYGLEGLHHPEYTGVWVGEKKIAAIGVKFSSGW
                     ITSHGFALNVSTDLTWFDRITPCGIREFGVTSLQRELGHQVELAEVERRVVEHFRETF
                     S"
     gene            2081..3103
                     /locus_tag="VFT03_10585"
     CDS             2081..3103
                     /locus_tag="VFT03_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848634.1"
                     /translation="MSVGKWQVRGPILLITALTLAVLTVVIVTGSSSAQTKQQTYDAC
                     QPGEDTITASRLPEAVSLQRCPVGERVIRDNGVGTVLPDPGQSVFVDATTTEGFQELE
                     VTRYKDGTVDLNHVGDESEDAQTSPDLVTAASGPSECSDRAFNLRDYKAFVVLRGYFN
                     TNSTPNGLSRRAAVRAVRRGTADVFDSRNNCHMGDRVPDRIGMSYEGSINARAQVGTG
                     GNCIGQDRKSVVSFGTLRSSALAVTCTERHLIDGKWHTKWSDIMINRAHFKWTTRPQS
                     RSCNGRKYDVESTVAHERGHTFGLDHVSESTHGNLTMSDRSNGPCQLSERSLGRGDVL
                     GLARKY"
     gene            complement(3260..>3626)
                     /locus_tag="VFT03_10590"
     CDS             complement(3260..>3626)
                     /locus_tag="VFT03_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU4848635.1"
                     /translation="TGEPRITFLVGLAAAVGAAISMGFSEGLSDDGTLTGRGHPFLRG
                     SITGVATFIGGILHTLPFLIPHVNLALYVAFGVVGFELLAISFIRYRYFEMSFLMSAM
                     QVILGGALVFASGILIGSA"
BASE COUNT          721 a         1160 c         1152 g          593 t
ORIGIN      
        1 atccctccct ctcggagata gtcggcgagg cctcgatggc tgtggaaggc cacccgatac
       61 atttctagga ggtttcaggt ctcgggcgtc aggtaggatt tggggggcgg cgttggcacc
      121 acccggcacc gctatcccgg agtcctacct gaaacctgat acctgagatg gaaaacacgg
      181 tacggatgct ctccgttacg gtaccataag gcgcatagtg cgagggagat aggagagccg
      241 tggcgcgcag acagatcaac ctcaagatcc tggacgacgg taccaagact ttcgaggtcc
      301 gtcatcgctg ggaagacagg cccgtcgccc cgccccctga cgggactccg accgagtacc
      361 tgcccttcag gcaacagagg ggcctgcatg gacggcccga gtggctgaag gcaagagccc
      421 ccgtcgggga gggctacacc gacataaaag agaccatgcg cggtctcaac ctccacacgg
      481 tctgtgagga ggcccgctgc ccgaacatcg gcgagtgctg gaacaacagg acagccacct
      541 tcatgatcct cggcaacgtc tgcacccgct cctgcggctt ctgcgccgtc ctcaccggca
      601 agcccacgga gctcgacctc gacgaaccca ggcgcgtcgc cgacgccgcc ggacagatgg
      661 gcctcaagca cgccgtaata acgagcgtga accgcgacga gttggccgac ggaggcgccg
      721 cggtcttcgc cgcgaccata cgcgagatcc ggacccacgt gccgggatgc gccgtcgagg
      781 tcctcacccc ggacttcaag ggggaccggg acgccataaa gaccgtcatc gacgcccggc
      841 ccgacacttt caaccacaac atcgagaccg tcccgcgcct gtacccggcg gtccgtcctc
      901 aagccaagta ccagcgctcc ctcgaggtgt tgcgctacgc caaggagcta aactcggaag
      961 ggctcaccaa gagcggtttc atggtggggt tgggtgaggt ggaagaggag atccaacgca
     1021 ccatgctcga cctgcgggag cacgacgtgg acatcctgac catcggccag tacctccgac
     1081 cttcggagaa ccacctgccg atgagccgct actacgcgcc aaaggagttc gccgcgttcc
     1141 gcaagctcgg cttcgagatg ggcttcaagc acgtcgagag cggaccgctc gtccgcagct
     1201 cctaccacgc ccacgagcag accgaggacg cccggagcag cgctctgggc gcgagcgtct
     1261 agaagcgccc gctgtggcgc tcgagaagca ggccccggcg tctttcatcg aggtccgccg
     1321 cctgcctggc ctcgtgccct acgcggaggc ttgggagctg caggcagagt tggtgcagct
     1381 ccgcaaggcg gattcggtcc ccgacaccct cctgctcctg gagcatccgc cggtcttcac
     1441 cgtgggacgg gcggcgaagg acgcatcgaa cctgggggcg ggggagggct acctgcgctc
     1501 gctcggcgcg gaggtgttct ggagcgaccg cggaggggac gctacgttcc acggccccgg
     1561 acagctagtc ggctatccta tcctgcggct gcgcgaccgt gacactcatg ggtacctgcg
     1621 ccggctggag gcgatcataa tccgcgcgct cgcggactac gggctcgaag ggttgcacca
     1681 tcccgaatat accggtgtct gggtcgggga gaagaagatc gccgccatag gcgtcaagtt
     1741 ctcctcgggt tggatcacct cccacggctt cgccctgaac gtctccaccg acctcacctg
     1801 gttcgaccgc attacccctt gcggtatccg cgagttcggc gtgaccagcc tgcaacgcga
     1861 gctgggacat caggtagagt tggccgaggt cgagcgaagg gtagtcgagc acttccgcga
     1921 gactttcagt tagcctgctc cctgaggctc tcccggcctc gcgaagtggt atgccgggat
     1981 gttaacatta tggtgacagc gcgacatctg gaggggctgt gccgatcttc gtacgatata
     2041 tggatagtca ccgcaccccc gcacacgata ggttacggtc gtgtcggtag gcaagtggca
     2101 ggtgcgcgga ccgatcctgc tcatcactgc cttgacgctc gccgtcctca cggtggtcat
     2161 agtcaccggg tcgtcgtcgg cgcagacaaa acagcagacg tacgacgcct gccagcccgg
     2221 cgaagacacc attaccgcct caaggctacc cgaagccgtt agcctgcaga gatgccccgt
     2281 cggggaaagg gtgatccggg acaacggcgt cgggaccgtt cttcccgacc ccggccagag
     2341 cgtcttcgta gacgcgacta cgaccgaggg ctttcaggag ctcgaggtaa ctcgctacaa
     2401 ggacggaacc gtcgatctga accacgtggg ggacgagtcc gaggacgcac agacaagccc
     2461 ggatctggtc acggcggcga gtggcccgag cgagtgctcg gatagagcgt tcaaccttcg
     2521 ggattacaag gccttcgtcg ttctgcgcgg gtacttcaac acgaattcga cccctaatgg
     2581 gctctcccgc agggccgctg tgcgcgcggt ccgcaggggg actgcagatg tcttcgattc
     2641 gcgaaataac tgtcacatgg gagatcgcgt tccggaccgg ataggcatgt cttatgaagg
     2701 ctccatcaat gcccgcgctc aggtaggtac gggcggtaac tgcataggcc aagacaggaa
     2761 gagcgttgtc tccttcggca cgttgcggag tagcgcgctc gctgtgacgt gcaccgagag
     2821 gcacttgata gatggcaaat ggcacaccaa atggtccgac ataatgatca acagggccca
     2881 cttcaagtgg accactcgcc cgcagtcccg ttcttgtaat gggaggaagt acgacgtgga
     2941 gagcactgtg gcgcacgagc gcggccacac cttcggcttg gaccacgtct cggagtccac
     3001 gcatggaaac ctgactatga gcgatcgctc caacggaccg tgccagctct cggagcggag
     3061 cctgggacgc ggcgacgtcc tgggtctggc ccgcaagtac tgatcagcac gcccgcttcg
     3121 caggctctca gcacgccacc tggcttcgcc aggcggcttc tcagcttctc tctgcactga
     3181 ctggctgaaa tgctgacaag cgagggccgc taggcccgag cgtgctgaca agccgaccga
     3241 agggaggcgt gctaacgggc tacgcgctgc cgattagtat ccccgaggcg aagaccagcg
     3301 cgccgccaag gatcacctgc atcgccgaca tgaggaagct catctcgaag taccggtagc
     3361 ggatgaagga gatcgccagg agctcgaagc cgaccacccc gaaggccacg tagagcgcga
     3421 ggttcacgtg cggaatgagg aacggcaggg tgtgcagaat ccccccgata aaggtggcga
     3481 cgcccgtgat ggaacccctc aagaaggggt ggccgcggcc cgttagcgtc ccgtcgtcgg
     3541 agagcccctc cgagaagccc atgctgatgg ccgctcctac cgcagccgcc aggccaacta
     3601 ggaacgtgat ccggggctcc cccgtc
//
LOCUS       DASTUV010000523         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_230878, whole genome shotgun sequence.
ACCESSION   DASTUV010000523 DASTUV010000000
VERSION     DASTUV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230878"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="VFT03_10595"
     CDS             complement(<1..1593)
                     /locus_tag="VFT03_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU4848636.1"
                     /translation="MRPEHETLAHTTFNPEERIDLLLRHLGTQREGLSEREAARRLEQ
                     HGPNEIQRREGPGRLRELARQFTHPLALLLWAAALFALVGGLAPLAAAIVAVIVLNAA
                     FAFFQELQAERATEALKKYLPPRARVRRGGRTIEVEATALVPGDLLLISEGDRLSADA
                     RLVSGSVEVDMSPLTGESQPVARGAARSRPAPSPLESDDLVFSGTLITGGEAEAVVYA
                     TGMATQLGRIAALTQRVQTEVSPLQVQVNKAARLIAAVAVGAGVVFLAVGTLAAGLPL
                     ADAVIFAIGLLVANVPEGLLPTITLALAVGVRRMARRRALLKRLTAVETLGSTDVICT
                     DKTGTLTEGRMVVSRFWADGEELAPDRHHTRVVEPFSGLLRTAVRCSNARISPTEDGW
                     ERSGDPSESALLAAAAILGEDVESVQAGRERRRRRLYHFDARVKRMTTADEEPTGELW
                     YHTKGAPLELLERCTAVRGAEGDRPLSLADGEKVRSAFERYAAEGLRVLGFAERRIGT
                     AQPAEDRGLAESGLTFLGLAALE"
     gene            complement(1590..2132)
                     /locus_tag="VFT03_10600"
     CDS             complement(1590..2132)
                     /locus_tag="VFT03_10600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4848637.1"
                     /translation="MKALAKPGDVVTDPTGVPVVSGDRGIIDLLDATRITCRQIQPQD
                     AAALRRFHRRLSQHSIHLRFFGAKPELSARKADYFTNVDWVNSFALVALDPEDPDEII
                     AIVSFYRERSTERAEYAAAVEDTWQGRGLGLALTRALITSALRRNVRVFTGMILPENA
                     LILDLPLELSPREKPGGLPA"
BASE COUNT          409 a          815 c          793 g          408 t
ORIGIN      
        1 ctcgagcgcc gcgagcccca ggaaggtgag cccagactcg gcgaggccgc ggtcttcggc
       61 gggctgggcg gtccctatcc tccgctcggc gaagcccagc accctgagcc cttctgcggc
      121 gtagcgctcg aaagcggagc gcaccttctc cccgtcggcc aggctcaggg gtctgtcccc
      181 ttctgctcca cggacggcgg tgcagcgctc gagcagctcg agcggcgcgc ccttggtgtg
      241 gtaccagagc tccccggtgg gctcctcgtc ggcggtggtc atgcgcttga cccgcgcgtc
      301 gaagtggtag agacgcctcc ggcgccgctc ccgtcctgcc tgcacactct cgacgtcctc
      361 gccgaggatg gcggccgcgg cgaggagcgc gctctccgaa gggtccccgc tccgctccca
      421 gccatcctcg gtgggactta tccgggcgtt ggagcaccgc acggccgtgc gaagaaggcc
      481 ggagaacggc tccacgaccc ttgtgtgatg gcgatccggc gcgagctcct ccccgtcggc
      541 ccagaagcgg gagaccacca tgcgtccctc ggtgagggtt ccggtcttgt ccgtgcagat
      601 aacatccgtc gagcccaggg tttccacggc ggtcagacgc ttcagaagtg cacggcggcg
      661 ggccatgcgc ctcaccccca ccgcgagcgc gagcgtgatg gtaggcaaga gaccctcagg
      721 gacgttggcg accaagagcc cgatggcgaa gatcaccgcg tcggcgagcg ggaggcctgc
      781 cgccagcgtg cccactgcca agaaaacgac cccagcgccg accgcgaccg cggcgatgag
      841 cctggcggcc ttgttcacct ggacctgcaa gggactgacc tcggtctgga cgcgctgggt
      901 gagcgcggcg atgcgcccga gctgggtggc cattccggtc gcgtagacta ccgcctcggc
      961 ctcgccgccc gtaatgagcg tgcccgagaa gaccaggtcg tctgactcca gaggcgaggg
     1021 tgcaggacgg gacctggcgg cgccgcgggc cacaggctgc gactcgccgg tgagcggcga
     1081 catgtccacc tcgaccgagc cgctgacgag acgagcgtcg gccgagagcc ggtctccctc
     1141 tgagataagc agcagatctc ctggcacgag cgcggtggcc tcgacctcta ttgtccgacc
     1201 cccgcgccgc acgcgtgcac gcggcggcag atacttcttg agcgcctcgg tcgccctctc
     1261 cgcctgcagc tcctggaaga aggcgaacgc cgcgttgagc acgatcaccg ccactatcgc
     1321 cgccgcaagc ggcgccagcc cacccacgag ggcgaacaac gccgccgccc acagcaggag
     1381 agccagggga tgggtgaact ggcgggccag ttcgcgcagg cgtccagggc cctcgcggcg
     1441 ttggatctcg ttgggcccgt gttgctcgag tctccgcgcc gcctctcgct cggagagtcc
     1501 ctcacgttgc gtcccgaggt gtctcaggag gaggtctatc cgctcctcgg gattgaaggt
     1561 agtatgcgcc agagtctcgt gctcaggcct catgccggca agccacccgg tttctcccgc
     1621 ggcgacaact ctaatggcag gtcgagtatc agggcgttct cgggcaggat catgcccgtg
     1681 aagacccgga cgttcctgcg caaggcagag gtgatcagcg ctcgagtcaa ggccagcccc
     1741 aggcccctgc cctgccacgt gtcctcaacc gccgccgcgt actcggcacg ctctgtgctc
     1801 cgctccctgt agaagctcac gatagctatt atttcatctg gatcttcggg gtccagcgct
     1861 accaacgcga agctgtttac ccaatcgacg ttcgtgaagt agtctgcctt cctggcgctc
     1921 agctccggct tggccccgaa gaagcgcaag tggatagagt gctggcttag gcggcgatgg
     1981 aagcgcctga gagcagccgc gtcctgaggc tggatctgcc ggcaggtaat tcgggtcgcg
     2041 tcgagcaggt cgatgatccc tctatctccg ctcacgaccg ggacaccagt cggatctgtc
     2101 accacgtctc ccggctttgc cagcgctttc atcgtctgct cctatccgct aaggcgcgta
     2161 agaaccttgt gcgtggactc tagccgaagg ggagctcgcg accttgagca gaatgggcta
     2221 tctgcctagt caaagcggag aagggaaagc cttcgccact tggggctacg aaccgttggg
     2281 tggtagaacg gacgaactcc tggcaagcga atctagccca caagaaattg gtgtggtgca
     2341 ccgagagaga agggcgagtc atcgacttct ggatcgcttt ctcgaacgta atcatcatca
     2401 tcgtggggag gctcattcgg aaagc
//
LOCUS       DASTUV010000524         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_280560, whole genome shotgun sequence.
ACCESSION   DASTUV010000524 DASTUV010000000
VERSION     DASTUV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280560"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFT03_10605"
     CDS             <1..740
                     /locus_tag="VFT03_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEU4848638.1"
                     /translation="TEPFRHATPGERLRLPEGATVTLHAPYPGHTAKGEGSRLWLSTL
                     DLPHPLDEYLDRHGFPIRYGYVGESWPISYYQTAYATEAGSAEMPSAGRAFTPELITR
                     LVAQGVQFAPLLLHTGVASLEEDEPPYEEFYRVPPETARLVNAARTSGRRVVAVGTTA
                     VRALETVTDESGTTHPGEGWTRLIVTPERGIRAVDSMLTGLHEPRSSHLAMLEALAGR
                     EHLRAAYGEALRESYLWHEFGDLHLIL"
     gene            complement(743..1456)
                     /locus_tag="VFT03_10610"
     CDS             complement(743..1456)
                     /locus_tag="VFT03_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848639.1"
                     /translation="MLLLEFVVLGCGGEQSSRHAGSPTGEGAKVQESGDHVSDTKVIV
                     TSDDASLPDGCNTRQIAELAIEFVEAFNRGDEERLSRLFFVSEGPSPPDFSEAGYFPW
                     SWYSVSEIGEDGRIENGFVTYDKGELLSYFAERHRHGERLELIKVSSTRAGLLDEENN
                     VGIVYVLTRDAEDLDPGLGGPSRIAFGKGAINCASRQLFVWSMEMREGDERTPREAAG
                     WLCTDPPGWRPGDAVVACA"
     gene            complement(1625..2248)
                     /locus_tag="VFT03_10615"
     CDS             complement(1625..2248)
                     /locus_tag="VFT03_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4848640.1"
                     /translation="MTPTRAFVVAPAPVTRAGLRSMLANAEGTDVRVVGEASSPTDSS
                     ELSEADVVLLANEELLEEASFAVAEDGTQALVLISEEDHAVSRLRAMQLRGWGVVPPD
                     APPEELAAAVAAAGQGLIVLPKAVTERLLHEPAASAEELSETLTAREREVLDLLGHGL
                     SNKLIGRELHISEHTVKFHISSLYAKLGVNNRAEAVSRGARYGLISL"
     gene            complement(2245..>2563)
                     /locus_tag="VFT03_10620"
     CDS             complement(2245..>2563)
                     /locus_tag="VFT03_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU4848641.1"
                     /translation="GAQQRRLRLGVRIVPVEIPASLCAADPEMPEAGLMVAALEAGSP
                     AERAGLLVGDVLLGVDGRTPGDVESLLDAIAGAREAIRLRLMRGGKISVVDVDLKAPG
                     RAA"
BASE COUNT          423 a          925 c          815 g          400 t
ORIGIN      
        1 ccacggaacc gttccgccac gccacccctg gcgagaggct gcgcctcccg gagggagcca
       61 ccgttacgtt acacgccccc tatccagggc acacagctaa gggcgaaggg tcgcgtctat
      121 ggctctcgac cctcgatctg ccgcatcctc tcgacgagta cctcgaccgc cacgggttcc
      181 cgatccggta cggttacgtc ggggagagct ggcccataag ctactaccag acagcctacg
      241 ccacggaggc cggcagcgcg gagatgccct cagccggaag ggcgttcacg ccggagctca
      301 tcacgcgcct ggtcgcgcag ggcgtacagt tcgcacccct gctactgcac accggcgtgg
      361 caagcctcga ggaggacgaa cccccatacg aggagttcta ccgcgtccca cccgagaccg
      421 cccgcctcgt gaacgccgcg cggacgtcgg gccgcagggt cgtcgcggtc gggacgacgg
      481 cggtgcgggc gctcgagacc gtcacggacg agagcggcac cacccacccc ggcgaaggct
      541 ggacgcgcct gatcgtcacg cccgagcgcg gcatccgggc tgtggactcg atgctcacgg
      601 gcctgcacga gccccgctcc tcccacctgg ccatgctcga agcgctcgcc ggccgcgagc
      661 acctgcgcgc cgcgtacggc gaagccctgc gggaaagcta cctctggcac gagttcggcg
      721 acctgcattt gatcttgtag gttcaggcgc aggcaaccac ggcgtcgccc ggacgccagc
      781 cgggcggatc ggtgcatagc cagccggctg cctcccgcgg ggttctctca tctccctctc
      841 tcatctccat gctccacacg aagagctggc ggctcgcgca attgatggct cctttgccga
      901 aggcgatccg ggacggaccg ccgagcccgg ggtcgaggtc ttcggcatcg cgagtgagga
      961 cgtagacaat gcccacgttg ttctcctcgt ccagcaagcc agcccgcgtc gagctcacct
     1021 ttatcaactc gagacgctct ccgtgcctgt gcctctcggc gaagtagctg agcaactcac
     1081 ccttgtcgta ggtgacgaat ccgttctcga tccttccgtc ttccccgatc tcgctcaccg
     1141 agtaccagga ccacgggaaa taccccgcct cggagaaatc cggggggctc gggccctccg
     1201 agacgaagaa gaggcgggac aaccgctcct catcaccccg gttgaaggct tccacgaact
     1261 cgatcgccag ctctgctatc tggcgcgtgt tacacccgtc cggcaaagag gcgtcgtcgc
     1321 ttgtgacgat taccttcgta tcggacacgt gatccccgct ttcctggacc ttcgctccct
     1381 cgcccgtagg cgatccggcg tgccgcgagc tctgctcccc cccgcacccc agcacaacga
     1441 actcgaggag cagcaccggt aagaccctag tgcttgtacg acgcccgctc actggttcct
     1501 ttccctaccg ccttgcgggg cgggtccact ctacacccgg gagaaggtct acaccacgcc
     1561 aaccatccgg gcgcgcaccc ccatagatag gaccttcgcc ggtgttcgac gctcgaacgt
     1621 tctcttagag ggagatcagt ccgtaacggg cgccgcggct gacggcttcg gcgcggttgt
     1681 tgaccccgag cttggcgtag agggaagaga tgtggaactt gacggtgtgc tcgctgatgt
     1741 gaagctcgcg gccgatgagc ttgttggaga ggccgtggcc gaggagatcc aggacctccc
     1801 gctcgcgcgc ggtgagggtc tcggagagct cctcagcgga ggccgcaggt tcatggagca
     1861 acctttcggt cacggctttg gggagcacga tcaggccctg ccccgccgcg gcgaccgcgg
     1921 ccgcaagctc ctccggcggg gcgtcgggcg ggacgacgcc ccagcccctt agctgcatcg
     1981 cgcgcagcct ggagacggcg tggtcctcct cggagatcaa gacgagcgcc tgcgtgccgt
     2041 cctccgccac ggcgaaagag gcctcttcga gcagttcctc gttagccagc aggacgacat
     2101 cggcctcgga gagctccgag gaatccgtgg gagaggaggc ttcccccacg acgcgtacgt
     2161 cggtcccctc ggcgttcgcg agcatcgagc gcagccccgc ccgggttacc ggcgccgggg
     2221 ccacgacgaa cgcccgcgtc ggtgtcacgc ggcgcgcccc ggggccttca gatccacgtc
     2281 cacgaccgag atcttaccgc cgcgcatcag acgcaggcgg atagcctccc tggcgccggc
     2341 aatggcgtcg agcaaggatt ccacgtcgcc gggcgtgcga ccgtccacgc cgagcagcac
     2401 gtcgcctacg aggaggccgg ccctctcggc gggactgccc gcttcgaggg cggcgaccat
     2461 gagacccgcc tcgggcatct cggggtctgc ggcgcacagg gaagcaggga tctcgaccgg
     2521 cacgatcctg acgcccagcc ggagccgccg ctgctgcgcg ccc
//
LOCUS       DASTUV010000525         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1410893, whole genome shotgun sequence.
ACCESSION   DASTUV010000525 DASTUV010000000
VERSION     DASTUV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410893"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFT03_10625"
     CDS             complement(<1..859)
                     /locus_tag="VFT03_10625"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU4848642.1"
                     /translation="MARRVSRARSAIRWERHRRTFEEYIRAMRFTEEPRLEGVEEAMR
                     YSMLAGGKRVRPTLCMEVAWVCGAEPAHVLPSAAAIELIHTYSLIHDDLPAMDDDDFR
                     RGRPTAHKKFGEAMAILAGDAFFGEALTLITVRQEGSCEQILEVVRELADSTGVNGMV
                     GGQVMDMDQTGVGAEVDLETLYMIHKYKTGALIKSSARIGAILAGAGEDAQAAVSEYA
                     SELGLCFQIVDDVLDATSTRDALGKSAGSDAEQGKATFVGAYGLDGARSEADRVLERA
                     LAALGRLDGD"
     gene            complement(843..1256)
                     /gene="nusB"
                     /locus_tag="VFT03_10630"
     CDS             complement(843..1256)
                     /gene="nusB"
                     /locus_tag="VFT03_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU4848643.1"
                     /translation="MSRRKARKQAFFVLYQSDVNEVPVEATFERWRAYRGNLDEYAVR
                     LAGGVEGEKDDLDALLAQVSVGWPVHRMSAVDRAILRLALYEMRSVDDVPPEVAVSEA
                     VELAKGFSGEEAPAFVGGVLRGAVDRVGVGHGETG"
     gene            complement(1260..1817)
                     /gene="efp"
                     /locus_tag="VFT03_10635"
     CDS             complement(1260..1817)
                     /gene="efp"
                     /locus_tag="VFT03_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU4848644.1"
                     /translation="MISTNQFRNGSAIRVDGKRFTIIYFQHVKPGKGGAFVRTRLRNI
                     DTGALIEKTFRAGEKLESVRTESRPMTYLYRDGDLLYFMDTETYEQIAVSLEVVGDAV
                     GYIVPNGTVSVLSADGEVVSVEPPAHVDLEVAETDPGLKGDTATGGSKPATLETGVVV
                     QVPLFVNAGDRVRVDTRTNEYLTRV"
     gene            complement(1967..2380)
                     /locus_tag="VFT03_10640"
     CDS             complement(1967..2380)
                     /locus_tag="VFT03_10640"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU4848645.1"
                     /translation="MFVLNGINLGSLGERRPEVYGTQTLGDIEDLLRAGFPDVDFEFR
                     QTDYEGEFVAWIRDAAGSAGLVVNPGAWTHYSYAIHDALEAVSVPKVEVHLSNVHARD
                     EGWRRESVVSPVVDATVAGMGASGYLAAVRYLLER"
     gene            complement(2382..>3072)
                     /locus_tag="VFT03_10645"
     CDS             complement(2382..>3072)
                     /locus_tag="VFT03_10645"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEU4848646.1"
                     /translation="SLLAMVDSSVGGKVGVDLSEGKNLLGSFLRPRLVAADLDWLETL
                     PEREVSGGLAEVVKMGLLSGGRYFEDLDGVGAARSRDLDALRALVLDSVRFKAQVVAE
                     DELEAGRRAILNYGHTIGHGLEAATGYTLPHGEAVATGMVAAAHLAREKLGTDLVSLH
                     EDLLASAGLPTRVPELDPQKVLAAMGRDKKRRAAEEAHRFVLLEGVGRPVWGVRVREE
                     EARRAIGAVVG"
BASE COUNT          492 a         1122 c          925 g          533 t
ORIGIN      
        1 tgtcgccgtc cagcctaccg agagccgcga gggccctctc gagcaccctg tctgcctcgc
       61 tcctggcgcc gtcgaggccg taagcgccaa cgaacgtagc cttgccctgc tcggcgtcgc
      121 tccccgcgct cttgcccaga gcgtcgcgcg tggaggtggc gtcgagcacg tcgtccacta
      181 tctggaagca gaggcccagc tccgaggcgt actctgatac ggcggcctgc gcatcctcgc
      241 cagccccggc gaggatcgcc cctatgcgcg ccgaggactt gatgagagcg ccagtcttgt
      301 acttgtggat catgtacagc gtctcgaggt ccacctctgc tcccaccccg gtctggtcca
      361 tgtccatgac ctgccccccg accatcccgt tgacccccgt ggagtcggcc agctcacgca
      421 ctacctcgag gatctgctcg cagctcccct cctggcgcac ggtgatgagc gtgagcgcct
      481 cgccgaagaa ggcatcgccg gccaggatcg ccattgcctc gccgaacttc ttgtgggccg
      541 tgggcctccc ccgcctgaag tcgtcgtcgt ccatagcggg gaggtcgtcg tgtatcaggg
      601 agtaggtgtg gatgagctct atggcggcgg ccgaagggag cacgtgggcc ggctcagccc
      661 cgcagaccca ggccacctcc atgcacagcg tgggccgcac gcgcttgccg cccgcgagca
      721 tcgagtagcg catggcctcc tcgaccccct cgaggcgcgg ctcctccgtg aagcgcatag
      781 cccggatata ctcctcgaag gtgcggcggt gcctctccca gcggatcgcg ctgcgcgctc
      841 ggctaacccg tctcgccatg tcccaccccc acccggtcca ccgctccccg caagacccct
      901 ccaacgaaag cgggagcctc ctcgcccgag aaccccttgg ccagctccac agcctcgctg
      961 acggcgacct cggggggaac atcgtccacg gagcgcatct cgtagagcgc cagccgcagg
     1021 atagcgcggt cgacagcgct catcctgtgc acgggccagc cgaccgagac ctgcgccagc
     1081 agcgcgtcca ggtcatcctt ctccccctcc accccgcctg ccagccgcac ggcgtactcg
     1141 tcgagattgc ccctgtacgc ccgccagcgt tcgaacgtcg cctcgacggg gacctcgttc
     1201 acgtcgctct ggtagaggac gaaaaacgcc tgcttgcgcg ccttgcgccg gctcaaggct
     1261 cagaccctcg tcaggtactc gttcgtgcgc gtgtccaccc gcacccggtc accggcgttc
     1321 acgaagagcg gcacctgcac cacgaccccg gtctccagcg ttgcgggctt gctgcccccg
     1381 gtcgccgtat cgcccttgag gcccgggtcc gtctcggcta cttcgaggtc gacgtgcgcg
     1441 ggaggctcca cgctcaccac ctcgccatca gccgagagca cgctaacggt gccgtttggc
     1501 acgatgtagc cgaccgcatc ccccaccacc tcgagggaga cggctatttg ctcgtaggtc
     1561 tccgtgtcca tgaagtacag cagatccccg tccctgtaga ggtaggtcat gggtctcgac
     1621 tcggtcctga cgctctcgag cttctcgcct gccctgaagg tcttctcgat cagcgctccc
     1681 gtgtctatgt tcctcagccg tgtcctgaca aacgctccgc ccttgcctgg cttcacgtgc
     1741 tggaagtaga tgatggtgaa gcgcttgcca tctacccgaa tggcacttcc gttcctgaac
     1801 tggttggtgg atatcatgcc tgaaactccc cttcgaaacg cctcgtcgta gctgtacgcc
     1861 accacaaacc cggcgcagac gccgcgatat tatcgcacag attagccccc agccatcagc
     1921 ttctcagcag aatatcgaaa cccgagcggt gttctcgagg gcgtattcac cgttccagga
     1981 ggtagcggac cgccgccagg tacccggagg cgcccatccc ggctacggtc gcgtccacga
     2041 caggtgagac gacagactcg cgccgccacc cctcgtccct ggcatggacg ttggagagat
     2101 gaacctccac tttggggacg gataccgctt ccagagcgtc gtgtatggcg taggagtagt
     2161 gggtccaggc cccggggttg acgacgagcc cagccgagcc ggcggcgtcc cggatccagg
     2221 ccacgaactc accctcgtag tcggtctgcc tgaactcgaa gtcaacgtcg ggaaacccgg
     2281 cgcgcaagag atcttcgatg tccccaagcg tctgcgtacc gtagacctcg gggcggcgct
     2341 cacccagcga gcccaggttg atcccgttga ggacgaacac tctagccaac gacggcccct
     2401 atcgccctgc gtgcttcttc ttcgcgcacc cgcactcccc agacgggcct gcccacgcct
     2461 tcgagcagga cgaacctgtg cgcctcctca gcggcgcggc gtttcttgtc gcggcccatc
     2521 gcagcgagga ccttctgtgg gtccagctcc ggcacccttg tggggagccc ggcggaggcg
     2581 agcaggtcct cgtgcaggct aacgaggtct gtgcccagct tctcacgggc gaggtgggcg
     2641 gcggcgacca tccccgtggc gacggcctcg ccgtgtggca gcgtgtagcc cgtggcggct
     2701 tccaggccgt gacctatggt gtgaccgtag ttgaggatgg cgcgccgacc cgcctctagc
     2761 tcgtcttcgg ccacgacttg cgccttgaag cggacggagt ccaggaccag ggcgcgcagg
     2821 gcatcgaggt ccctggacct cgccgccccg accccatcga ggtcctcgaa gtaacgtcct
     2881 cctgagagca ggcccatctt gaccacttcg gcgagtcccc cggagacctc gcgctccggg
     2941 agcgtctcta gccagtcgag atcggccgcg accaggcgag gccgcaggaa gctcccgagc
     3001 aggttcttgc cctctgagag gtcgacgccg accttcccgc ccacggagct gtccaccatg
     3061 gccagaagcg ag
//
LOCUS       DASTUV010000526         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1304013, whole genome shotgun sequence.
ACCESSION   DASTUV010000526 DASTUV010000000
VERSION     DASTUV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304013"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1057)
                     /locus_tag="VFT03_10650"
     CDS             complement(251..1057)
                     /locus_tag="VFT03_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU4848647.1"
                     /translation="MLELQRNVIFGQFVDTGSVIHRLDPRFKIAAVVVMLAASFLIDD
                     FAGFAVAFALLALVQISSHIPLGYLLRGSQLFFGFLLFILLFQVLFYAGDVSEYLWRW
                     GILSVSPEGLRYAAILGLRVVFLYYVTTMLMLTTSLVDLTNGLELIFGPLQRIGVPVN
                     ELVLVFVIAIKFVPIFLEEIERLARAQTARGVPFDEGGAIRRARRIGRLLVPIFISGF
                     ARADLLSTAMHTRCYRGGRYRTKLRQMKASAADWAMLALVAAWMAAAWLV"
     gene            complement(1057..1929)
                     /locus_tag="VFT03_10655"
     CDS             complement(1057..1929)
                     /locus_tag="VFT03_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848648.1"
                     /translation="MKHDATPIIELRNFSFTYLEGTPRANEAIRDVSLEIQRGERVAI
                     VGPSHSGKSTIVDAFAHLLRLKRGQVFYEGADVAAPGYDRAALRREVGIVFQQPDSQI
                     IEDVVGADVAFGPTAAGLPAEETRRRVEESLEALGLPYPEYRLRYVHALSGGQRRRVA
                     IAGVLAMHTTVLVLDEPMAGLDPRGRRELLGLLRRLADDRDLTLVVCSASLGDVSLLC
                     DRVVVLDEGSVAMDGSLSEVLRQAERLADLDITLPEPTAGARELRKLFPDLPADVLTE
                     EELETELLRKLGAA"
     gene            complement(1926..>2836)
                     /locus_tag="VFT03_10660"
     CDS             complement(1926..>2836)
                     /locus_tag="VFT03_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848649.1"
                     /translation="SSQTKSAATRIPTQRDGDVIEIRDLSFSYRTGEADAVPALRGLD
                     LRVEPGQFVAIIGHNGSGKSTLVKLLCAVLYPTEGEIRIEGIPVSEEHQWEVRERVAI
                     VFQDPDDQLVMNRVADDVAFGPENLGLPREEIEERVAFALGALGLEGIAGTLIEDLSP
                     GQKQRVAIAGSLAMRPRFLMLDEPTSLLPAPTAKRLISTVKELNRREGMGVLHVTHSM
                     SEAALFDRVVVMDEGRKVMDGTPAEVFRRVEELRSTGLDVPLAASLAHRLRSRGVPLV
                     GDILSDADLREAISALRGSRAESAGP"
BASE COUNT          506 a          931 c          959 g          440 t
ORIGIN      
        1 tcagcatttc agcttcttct gccgttgggc tcatacccgg tcacgcttgg agagacactc
       61 tttgggcata tccttggact gacgcaggga agcaccatct cgcgtcctcc ttggttttaa
      121 gctgagaagc cgcctgaggc gaagccaaag gctgcgtgct gagagcgagg ccctaaaagg
      181 gccgaagcgt gctgagaagc cgaccgaagg gaggcgtgct gacaagcgga ggccgtaagg
      241 ccggagcgtg ctagaccagc cacgcggcgg ccatccaggc ggctacgagg gcgagcatag
      301 cccagtcggc cgccgaggcc ttcatctggc ggagcttggt gcggtatcgg ccgccgcggt
      361 agcagcgagt atgcatggcg gtactgagta ggtcggcgcg ggcgaacccg cttataaaga
      421 tggggacgag caggcgtccg atgcggcgag cgcgcctgat cgcgcctccc tcgtcgaagg
      481 ggacgccgcg ggcggtctgg gcgcgggcga ggcgttcgat ctcctcgagg aagatgggga
      541 cgaacttgat cgcgattacg aacaccagga ccagctcgtt gaccggaact ccgatcctct
      601 gcagggggcc gaagatcaac tccagcccgt tcgtgaggtc cacgagcgag gtcgtgagca
      661 tgagcatggt cgtgacgtag tagaggaaga cgacgcgcag gcccagaatc gccgcgtacc
      721 tgagcccttc cggcgagacc gagagtatcc cccaccgcca caggtactcg gagacgtccc
      781 ccgcgtagaa aaggacctgg aataacagga tgaagagcag gaagccaaaa aagagctggg
      841 agccccgaag caggtagccc agggggatgt gcgacgagat ctgcaccagg gcgaggagcg
      901 cgaaagctac cgcgaagcca gcgaagtcgt ctatgaggaa cgaggcggca agcatcacga
      961 cgacggccgc gatcttgaag cgggggtcga gcctgtggat cacagacccc gtatccacga
     1021 actgcccgaa gatgacgttg cgctggagct cgagcactat gccgccccaa gcttcctgag
     1081 gagctcggtc tccagctcct cttcggtgag cacatccgct gggagatccg ggaacagctt
     1141 cctgagctcc ctagcaccgg ccgtcggctc ggggagcgtg atgtcgagat ccgcgagcct
     1201 ctccgcctgc cgcaggacct cactcaggct cccgtccatg gcgacgctac cctcgtcgag
     1261 gaccacgacc cgatcgcaca ggaggctcac gtcgcccaga gaagcggagc acaccacgag
     1321 ggtgaggtcg cggtcgtcgg cgagccgccg cagcagcccg agtagctccc gccggccccg
     1381 cgggtcgagg ccggccatcg gctcgtcgag gacgagcacg gtggtgtgca tcgcgagtac
     1441 gccggctatg gcgacgcggc ggcgctgccc gccggagagc gcgtgaacgt acctgagcct
     1501 gtactcgggg taaggtaagc ccagggcctc caggctctcc tcgacccgcc ggcgcgtctc
     1561 ctcggcagga aggccggcgg cggtcgggcc gaaggccacg tccgccccga cgacgtcctc
     1621 tatgatctgc gagtccggtt gctggaagac tatgccgacc tcgcggcgca gagccgctcg
     1681 gtcgtagcct ggagccgcca cgtccgcgcc ctcgtagaag acctgcccgc gcttcaggcg
     1741 caggaggtgg gcgaaggcgt ccacgatggt ggacttgccc gagtgcgacg ggcccactat
     1801 cgcgacgcgc tcgccgcgct ggatctcgag cgagacgtcc cggatcgcct cgttcgcccg
     1861 cggcgtgccc tcgaggtacg tgaagctgaa gttcctcagc tcgatgatcg gcgtcgcgtc
     1921 gtgcttcatg ggccggcaga ctccgcgcgg gagccgcgga gagcggagat cgcctcacgc
     1981 aggtccgcat cactcaggat gtcgcccacg agcggcacgc cgcgggagcg gaggcggtgg
     2041 gccaggctcg cggcaagcgg tacgtcgagt ccggtactcc gcagctcctc cacgcgccgg
     2101 aatacctcgg ccggggtgcc gtccatgacc ttccggccct catccatgac caccacgcgg
     2161 tcgaagagag cggcctcgga catcgagtgg gtgacgtgca ggacgcccat cccctcccgg
     2221 cggttcagct ctttgaccgt ggagatcaga cgcttcgccg tcggagccgg gaggagcgag
     2281 gtcggctcgt ccagcatgag aaaccgcggc cgcatggcca gagagccggc gatcgcgacc
     2341 cgctgcttct gtcccggaga gaggtcttct atgagggttc ccgcgatgcc ttcgagcccg
     2401 agcgccccca gcgcgaacgc gacgcgttcc tctatctcct cgcgcgggag gccgaggttt
     2461 tcgggcccga aggccacgtc atccgcgacc cggttcatga ctagctggtc gtcggggtcc
     2521 tggaagacga tcgcgacccg ttccctgacc tcccactggt gctcctcgct caccgggatg
     2581 ccttctatgc ggatctcgcc ctccgtaggg tacaggacgg cacacaggag cttgaccagc
     2641 gtgctcttcc ccgagccgtt gtgaccgatg atcgcgacga actgccccgg ctccaccctc
     2701 aggtcgagcc cgcgcagcgc cggaaccgcg tcggcctcac ccgttctgta ggagaacgag
     2761 aggtcacgga tctcgatcac gtccccgtcc ctctgcgttg ggattctagt agcggcgctc
     2821 ttggtctgac tcgatc
//
LOCUS       DASTUV010000527         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_338707, whole genome shotgun sequence.
ACCESSION   DASTUV010000527 DASTUV010000000
VERSION     DASTUV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338707"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VFT03_10665"
     CDS             <1..1076
                     /locus_tag="VFT03_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848650.1"
                     /translation="TIAASLQASLSQVAATASLYYLFYGLMQPVWGMLSDRLGRVRVM
                     RLTLFGVAVAGVVSALAPNLTVLIAARALAGGLFAAVIPASLVYIGDTVGISYRQKAL
                     ADLMAASAVGTAVATVLGGLAAYLDGWRLAFAAPALAGAALAVLLARLPEPKAFSEQK
                     REGPLVQVRRLIVQPWALVVVGIALVEGAVILGGLTFLAPSLESGGASSAVAGLAVGL
                     YGLAVLGWTRAVKLVANRLGASTLILIGGGMLALGYTAGAIEQSLAGVSAAAVFIGGG
                     FAFMHSTLQTWATEVVPESRATVISLFAAALFTGSGFAALAAAPLAEAGSFDLLFALA
                     AFVAVPLGLFASLARRLYPQHPQ"
     gene            complement(1115..1486)
                     /locus_tag="VFT03_10670"
     CDS             complement(1115..1486)
                     /locus_tag="VFT03_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU4848651.1"
                     /translation="MILRLWRARINRARIEEYRSFEAERCIPMLHKQPGLLGVLFLRQ
                     AEDHAASLTIWEDRGAVEALQSSPSYRHTIHELAESALLAGDQSVELLEVESGDFRLD
                     ALVRTLDQTRFSKRRRADSNR"
     gene            complement(1765..>2709)
                     /locus_tag="VFT03_10675"
     CDS             complement(1765..>2709)
                     /locus_tag="VFT03_10675"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU4848652.1"
                     /translation="LVHIGMADITYERDDIDAAERELEMGIGLTERTREVGELVRGYV
                     TLSKVKLARGDEEGAFEAAREAERVARTSGANLQISIAAAWTARLHIARGDLEETAAL
                     EQERATNADSGASQAVDRLTSARLLHARGRHDEALRLLRELREAAEAAGRIGTVIEIL
                     SLQALALWTSNEKEQGVGTLTQALAMAEPEGFVRTFVDEGPAMSELLSEVLQARQRGH
                     LDATGRVSTQYLAKLLAVLAQGASANGANARLAEPVSERELEVLALIAAGETNGEIAG
                     KLFVSISTVKTHINNLYRKLGARSRTQAVARARELGLL"
BASE COUNT          380 a          925 c          860 g          544 t
ORIGIN      
        1 tgaccattgc tgcctccctc caggcgtcgc tgagccaggt cgcggccacg gccagcctct
       61 actatctgtt ctatggcttg atgcagcccg tgtgggggat gctctcggac cggctcgggc
      121 gcgtgcgtgt gatgcgcctg acgctcttcg gggtcgcggt ggcgggggtc gtctcggcgt
      181 tggccccgaa cttgactgtg ttgatagccg cgcgggcgct cgccggggga ctcttcgcgg
      241 cggttatacc ggcctccctc gtctacatag gcgatacggt cgggattagc taccggcaga
      301 aggcgcttgc ggatctgatg gcagcctcgg ccgtgggtac ggccgtggcg acggtgctcg
      361 gggggcttgc cgcatacctc gacggctggc ggctcgcttt cgcggctcct gccctcgccg
      421 gtgctgcgct tgccgttctg ctcgcccggc tgccggagcc gaaagccttc tccgagcaga
      481 agagggaagg gccactggta caagtccgcc gtctcatcgt ccagccatgg gcactggtag
      541 tggtagggat agcgctggta gagggagcag tgatactggg cggccttacc ttcctcgctc
      601 cttcgctcga gagcggcggc gctagctccg cggtcgcagg gctcgccgtc ggactctacg
      661 ggctcgcggt gctcggctgg acccgggccg tcaagctcgt cgccaatcgc cttggtgcga
      721 gcacgctcat cctgatcggt ggcggtatgc tggcgctcgg ttacaccgcg ggcgccatcg
      781 aacagtctct cgcgggcgtc agtgccgctg ctgtattcat cggcggaggg ttcgccttca
      841 tgcactccac ccttcagacc tgggccaccg aggtcgtgcc cgagtcacgc gccaccgtga
      901 tctcgctctt cgccgccgcc ctcttcaccg ggagcgggtt tgccgccctg gccgcggccc
      961 cgttggcgga ggcaggttcc ttcgacctgc tcttcgcgct ggccgccttt gtggccgtcc
     1021 ctctgggact atttgcgagc ctggcgcgcc gcctgtatcc acagcatccg cagtaaagac
     1081 tgcaagccgc ctcacagcac ttgcaaggcg ggggtcagcg gttcgagtcc gctcgccggc
     1141 gctttgagaa cctcgtctgg tcgagcgtcc tcaccagcgc atccagacgg aagtctccgc
     1201 tctcgacctc caggagttcc accgactgat ccccggccag cagggcgctt tcggcgagct
     1261 cgtggatagt atgccggtaa gaaggcgagc tttgtagcgc ctccaccgcc ccacggtcct
     1321 cccagatcgt gagtgatgcc gcgtgatcct cggcctggcg caggaagagc accccgagaa
     1381 gcccgggctg tttgtggagc atcggaatac accgctcggc ctcgaagctc cggtactctt
     1441 cgatacgcgc ccgattgatc cttgcgcgcc acaagcgcag gatcatagct cctcctccat
     1501 gttcggcctc acccggtgtc cggtgatggg gatcaccaac ctcatgaaca ccccggtctc
     1561 cccgaccacc actccaaggg gcctccgcac aatatttatc atcgaagtgc tcccttcctg
     1621 cgaccttctg gtcgagcgca tctcgaggac cactctctta atatcgagga ccagggtggg
     1681 tggcatcggt cgaagggtgg atcggggggt ggagttccat ctgttgccgc cggtctcggg
     1741 cactatatcg agctacgggc tgtgctagag gaggcctaac tctctggcac gagccaccgc
     1801 ctgcgtacgg ctgcgcgccc ctagcttgcg gtaaaggttg ttgatgtggg tcttgacggt
     1861 gctgatggag acgaagagtt tgcctgctat ctccccgttg gtctcgccgg cggcgatgag
     1921 cgcgagcacc tccagctccc gctcgctcac tggctctgcg agccgtgcgt tggcgccgtt
     1981 ggccgacgca ccctgcgcga gaaccgccag gagcttcgcg aggtattgtg tcgagacacg
     2041 gcccgtagcg tcgagatgcc ctctctggcg ggcttgcagc acctcagaga ggagttcaga
     2101 catcgcggga ccctcgtcaa cgaaggtcct gacgaagccc tccggctccg ccatggcgag
     2161 ggcttgcgtg agggttccaa cgccctgctc tttctcgttc gaagtccata gcgccagggc
     2221 ctgcagcgaa aggatctcta tcacggtgcc tattcgccct gccgcttccg ccgcttcgcg
     2281 caactctctc agcagtcgga gcgcctcgtc gtgccggccc cgggcgtgga gcagcctcgc
     2341 cgaggttagc ctgtccaccg cctgggaggc gccagagtcg gcgttcgtag cacgctcctg
     2401 ctctaaggca gccgtctctt cgagatcccc ccgcgcgatg tgtagccgcg ccgtccaggc
     2461 cgcggcgatg gagatctgca ggttcgcgcc ggaggtgcgc gcgactcgct cggcctcgcg
     2521 ggctgcctcg aaggcaccct cctcgtcccc ccgtgccagc ttcaccttcg acagggtgac
     2581 atagcccctc accagctcgc cgacctccct cgtccgttcg gtcagcccta tgcccatctc
     2641 cagctcgcgc tccgctgcgt cgatgtcgtc ccgttcgtaa gtgatgtctg ccatccctat
     2701 gtggacgag
//
LOCUS       DASTUV010000528         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_504476, whole genome shotgun sequence.
ACCESSION   DASTUV010000528 DASTUV010000000
VERSION     DASTUV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504476"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFT03_10680"
     CDS             <1..386
                     /locus_tag="VFT03_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010826143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4848653.1"
                     /translation="VSYRGRSVQVTVNDRGPYVGRRDLDLSQGAAEYIGLTHAGVDYV
                     EYSRTGGYDPRYETYPADTGYSNSSGTESYASGDMTGGGTYVVQAGDTMSGIAAQLGT
                     SVDYLAAANGIANPDLIYIGQTLYY"
     gene            450..1478
                     /locus_tag="VFT03_10685"
     CDS             450..1478
                     /locus_tag="VFT03_10685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU4848654.1"
                     /translation="MAKDGAAPTFGLWYDFRQRLPLGSYEKFYAECFEEIEESERLGF
                     TGLWLSEHHFVDDGYLPSPLVVAAAVASRTRRLTIGTNVLLLPMHHPLRVAEDAAVVQ
                     LISGGRFVLGVGQGYVQHEFEALGFDRKYRPSLLEEGIEVIKHSWEVGGTGYEGKRWR
                     FDDLPFGPRPEKRPPIYVGAFADPAIDRAVRLADGFLASTGGGALADTYRRVREALAR
                     HGRDDEDFPFIASGVVYVHEDPERAFADVAPAISYQRSRYAEWGTDRDRPRPEPIRPE
                     DLPWERYLVGSPDEIAEGLIRLHQEAPYDHYCFWGRLPGITHEQALANARLFASEVMP
                     KVRSVAGK"
     gene            1475..2539
                     /locus_tag="VFT03_10690"
     CDS             1475..2539
                     /locus_tag="VFT03_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU4848655.1"
                     /translation="MKATPETLSPQQAAFLVRQRVARLATADAAGAPHAVPVCFAFSG
                     GEIFIALDDKPKDVAPARLKRVRNILENPSVALVADRYAEDWSLLAFVMVRGRARLLE
                     PGSEEHIAAIRLLRGKYHQYEEMRIEDNPVICIRPERVVSWGALDVAGSGEPAVLDAV
                     RGRRSVRRYLPKEVPEDLVEAVLEAGRWAPSPHGTQPWRFAVVTKEETRERLAGAMGE
                     EWRANLEMDGQAPEVVERRLQGSRRRLLDAPVLVLLCLYLDDLDVYPDAGRQESETTM
                     AVQSLGAAAQNMLLAAYDLGLDAGWMCAPLFCPEEVVEALALDQALVPHALLTLGYAE
                     GDPPKRRSRRPLDDLVVYRD"
     gene            2671..>3364
                     /locus_tag="VFT03_10695"
     CDS             2671..>3364
                     /locus_tag="VFT03_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4848656.1"
                     /translation="MALGAALWGTDAVLRVPLLEVMSPPAIVLSEHLVLMLYSVPAVA
                     LGWGVFRTLRAPQWAALLVIAWGGSALATLLFTTAFAFGNPTVVILLQKTQPLFAIAL
                     AHVLLRERLRWTYWPCFAVAVLGAYLISFDGFVEPFEALGSAQALPALLTLGAAVLWG
                     SSTALGRFVLKDMPFHTLTGARLLFAVPVLWAIVLVQGSLGEMGAGFAVEPARVVLLA
                     LIPGLLGLLLYYR"
BASE COUNT          493 a         1112 c         1192 g          567 t
ORIGIN      
        1 tggtgagcta ccgtggccgc tcggtgcagg tgacggtgaa cgaccgtggc ccctacgttg
       61 gcaggcgcga cctcgacctc tcgcaggggg ctgcggagta catcggcctc acccacgccg
      121 gcgtcgacta cgtcgagtac agccgcacgg gcggatacga cccccggtac gagacctacc
      181 cggcggatac gggctattcc aactcttcgg ggacggagag ctacgcctcg ggtgacatga
      241 cgggaggagg gacctacgtg gtgcaggcgg gggacacgat gtccgggatc gccgcccagc
      301 tggggacctc ggtggattac ctggctgcgg ccaacggcat agcgaaccct gacctgatct
      361 acatcggcca gacgctctac tactgagccg acggttcggg ctaccgcccg gtgtaaactt
      421 ggtgcgcaag ctcgagcata ggaggcgtct tggccaaaga cggtgctgcc ccgaccttcg
      481 gtctctggta cgacttccgg cagaggctcc cgctggggag ctacgagaag ttctacgcgg
      541 agtgcttcga ggagatcgaa gagtccgagc ggctgggctt cacggggctc tggctctcgg
      601 agcaccactt cgtggacgac gggtacctgc cttcgccgct cgtcgtggcg gcggccgttg
      661 cgtcccgtac tcggcgcctc acgatcggga cgaacgtctt gctgctgccc atgcaccacc
      721 ctctgcgcgt cgccgaggac gcggcggtgg tgcagctcat ctcaggcggt aggttcgtcc
      781 tcggggtcgg gcagggctac gtccagcacg agttcgaggc tctggggttc gaccgcaagt
      841 accggccatc gctgctggag gagggcatcg aggtcattaa gcactcctgg gaggtggggg
      901 gcaccggcta cgaggggaag cgctggcgct tcgatgacct gccgttcggg ccgcgaccgg
      961 agaagcggcc cccgatctac gtcggcgcct tcgccgaccc cgcgatagac cgtgccgtca
     1021 ggctcgcgga cggctttctg gcctcgacgg gcgggggcgc gctcgcggac acctacagga
     1081 gggtcaggga ggctctcgcg cgtcacggaa gggacgacga ggacttcccg ttcatcgcct
     1141 cgggggtggt ctacgtgcac gaggacccgg agcgggcgtt cgccgacgtg gcgcccgcca
     1201 tctcctacca gcgcagccgc tacgccgagt ggggcaccga cagggacagg ccgagacccg
     1261 agccaatacg gcccgaggac ctgccgtggg agaggtacct ggtgggaagt ccggacgaga
     1321 tcgcagaggg cctgatacgg cttcaccagg aggcgcccta cgaccactac tgcttctggg
     1381 ggcggctgcc cggcatcacc cacgagcagg cgctcgctaa cgcgcggctc ttcgcctccg
     1441 aggtgatgcc gaaggtccgt agcgtggcgg gtaagtgaag gccacgcccg agaccctgag
     1501 cccccagcag gccgcttttc tggtgcggca gcgcgtcgcc cggctcgcga cagccgacgc
     1561 ggcgggagcc ccgcacgccg tccccgtctg cttcgccttc tcagggggtg agatcttcat
     1621 cgccctcgac gataaaccca aagacgtcgc cccggcgcgc ctgaagaggg ttcggaacat
     1681 cctcgagaac ccgagcgtcg cgctcgtcgc ggaccgctac gccgaagact ggagcctcct
     1741 ggctttcgtc atggtgcgcg ggcgggcgcg gctcctcgag cctggcagcg aggagcacat
     1801 cgccgccata agactgttgc gcggcaagta ccatcagtac gaggagatgc ggatagagga
     1861 caaccccgtg atctgcatcc ggccagagag ggtcgtctcg tggggcgccc tcgacgtcgc
     1921 cgggagcgga gagccggccg ttctcgacgc ggtccggggc cgccgctcgg tgcggcgtta
     1981 tctacccaaa gaagtgccgg aggacctcgt ggaagcggta ctcgaggccg gacgctgggc
     2041 gccttctccg cacggcacgc agccgtggcg cttcgccgtc gttacgaagg aggagaccag
     2101 agagcggctc gccggggcga tgggcgagga gtggcgggcc aacctcgaga tggacggcca
     2161 ggctcccgag gtcgtcgaga ggcgcctcca gggctcgcgg cggcggctgc tcgacgcgcc
     2221 cgtgctcgtg ctcttgtgcc tgtatctcga tgacctcgac gtctaccctg acgccgggcg
     2281 gcaggagagc gagaccacga tggccgtaca gtcgctgggg gccgcggcgc agaacatgct
     2341 gctcgccgcc tacgacctcg gcctcgacgc gggctggatg tgcgctccgc tcttctgccc
     2401 ggaggaggtc gtggaggcgc tggcgctgga tcaggcactc gtcccgcacg ccctgctcac
     2461 gctgggttac gccgagggag accccccgaa gcgccgctcg cggcggccgc tagacgatct
     2521 ggtcgtatac agggactgag ggcgccgggc ttctagagga taggccgcgt gctccggacc
     2581 tatctgcggt tcggtatcat tgccggagtc ggatcacctg gaaggggcgg cgtgggctgg
     2641 agaaaaggta gttacattgg ggtcttgctc gtggccctgg gggcggcgct gtggggcacg
     2701 gacgcggttc tgagggttcc gctgctggag gtgatgtcgc cgccggcgat cgtgctctcc
     2761 gagcacctgg tgctcatgct ctactccgtg ccggccgtcg ctctggggtg gggcgtattc
     2821 cgcacgctga gggcgcccca gtgggccgcg ctgcttgtga tcgcctgggg tgggtctgcg
     2881 ctagcgaccc tcctgttcac gacggccttc gccttcggca accccacggt cgtgatactg
     2941 ctccagaaaa cccagcctct gttcgccata gccctggccc acgtcctgct gcgggagcgc
     3001 ctgcgctgga cctactggcc gtgcttcgcg gtagccgtgt tgggggcgta cctgatctcc
     3061 ttcgacggtt tcgtcgagcc cttcgaggct ctcggcagcg cccaggctct gccggcactc
     3121 ctcaccctgg gcgccgccgt tctctggggc tcctcgaccg cgctgggacg attcgtcctc
     3181 aaagacatgc ccttccacac cctgaccggg gcgcggctcc tcttcgccgt ccccgtcctc
     3241 tgggccatag tgctggtgca gggctccctc ggcgagatgg gcgctggctt cgcggtcgag
     3301 ccagcgcggg tggtcctgct ggccctgatc ccagggcttc tcggcctgct cctctactac
     3361 aggg
//
LOCUS       DASTUV010000529         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_946849, whole genome shotgun sequence.
ACCESSION   DASTUV010000529 DASTUV010000000
VERSION     DASTUV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946849"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1101
                     /gene="speB"
                     /locus_tag="VFT03_10700"
     CDS             151..1101
                     /gene="speB"
                     /locus_tag="VFT03_10700"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEU4848657.1"
                     /translation="MTRYEPADSFVTPRFSGVRTFMRLPNVQDLENADAAIVGAPFDT
                     GGTFRVGARFGPESIRSISHLLRRYNPSLGVGIFDHLSVIDYGDVPVVPGYIEESYER
                     IKEGLEPIHRAGVIPIVLGGDHSIALPELRAAAAAHGPLSLVQFDSHPDTWDAYFGQK
                     HTHGTPFRRAVEEGLLDPSRSIQVGMRGSIYDEGDWEDAIEMGFDLVPTDTVRKLGIE
                     ETITRIRERVGDARIYVSFDVDFVDPAFAPGTGTPEIGGFTSREAQEFVRGLAGLDIV
                     GCDVVEVLPPYDSAQITALLAANVAHEFLSLFALRRKNGG"
     gene            1094..2521
                     /locus_tag="VFT03_10705"
     CDS             1094..2521
                     /locus_tag="VFT03_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU4848658.1"
                     /translation="MDNLLLDYLVIALYFAVMIGAGYWGLRRARSADDYLVAGRRLGP
                     FMYVGTLSAVVLGGASTIGGVALGYENGISGMWLVFWIGMGVIALGILMSTRLSRLGV
                     YTVSEMLENRYGEASRLISAIIIAAYALMIAVTSTIAIGSVFNVVLPLSPSVAILVAG
                     GIVVAYSVAGGMWSITLTDFLQFLIMTVGIFFLLLPLTIAAAGGFSGMQEALPASYFD
                     LTAIGWQTIFTYFLLFFFGLMIGQDIWQRVFTARSPEIARWGSVAAGVYCLLYALAGA
                     LVGTAARVILPDLSNPDNAFARVASEALPAGITGLVLAAALAAVMSTASAGLLASSTL
                     LANDVYAGFIARGEHNRVLINRVTALLVGIVVLVISLIVSDVIGALTVAYDLLSGALF
                     VPIVGALFWRRATAAGALASMAAGSAVVVFFMVKDGLFANTPILYGLAASLVVFVVVS
                     LLTPKPSEESMRAWESKLSGSGTPP"
     gene            2570..>2919
                     /locus_tag="VFT03_10710"
     CDS             2570..>2919
                     /locus_tag="VFT03_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4848659.1"
                     /translation="MLESTRIKTEIPGPKSRALMERRHRAVSSGVGIATPMFAKEAKG
                     ALLTDVDGNTFIDFGGGIGVMNVGHTNARVAEAVREQAERLTHSCFYVTEYEQYIELA
                     ERLNALIPGDSEKRS"
BASE COUNT          496 a          972 c          910 g          541 t
ORIGIN      
        1 tgatagcgag cttgatcagc gccgccgtga tcctggttgc ctacctgcta cgccgccagt
       61 tcggaccccc ggagaaagat cccagggagg tggcccagag atagagatag aggacgcccc
      121 gcgcgctcct agacgaaagg agagggtccc ttgacccgct acgagccggc cgactcgttc
      181 gtgacgccgc gcttctcagg cgtgcgcacg ttcatgcggc tgccgaacgt ccaggacctc
      241 gagaacgccg acgccgctat cgtcggcgcc cccttcgaca ccggcggcac cttccgcgtc
      301 ggcgcccgct tcggccccga gtcaataagg tcgatctcgc acctcttgag acgctacaac
      361 ccctctttgg gcgtggggat cttcgaccac ctctcggtca tagactatgg cgacgtgccc
      421 gtcgtccccg gctatatcga ggagagctac gagaggataa aggagggact cgagccgata
      481 caccgcgcgg gggtcatccc catagtgctc ggcggcgacc actccatagc cctgccggag
      541 ctcagggccg cggccgcggc gcacggccca ttatccctcg tgcagttcga ctcgcaccca
      601 gacacctggg acgcctactt cggccagaag cacacgcacg ggacgccctt ccgccgcgcc
      661 gtggaagagg ggctgctcga cccttcgcgc tcgatacagg ttggcatgag aggttcgatc
      721 tacgacgaag gggactggga ggacgcaata gagatgggct tcgacctggt accaactgat
      781 accgtcagga agctcgggat agaagagacc ataacccgca tccgagagcg ggtcggagac
      841 gccaggatct acgtctcctt cgacgtggac ttcgtcgacc cggcgttcgc gccaggcacc
      901 gggacaccgg agataggggg cttcaccagc cgcgaggcgc aggagttcgt gcgcgggctc
      961 gcaggactgg acatcgtcgg ctgcgacgtg gtcgaagtat tgccccccta cgactccgcg
     1021 cagataacgg cgcttctcgc cgcgaacgtg gcgcacgagt tcttgagcct cttcgccctc
     1081 aggaggaaga acggtggata acctgctcct cgactatctg gtcatagctc tctacttcgc
     1141 cgtgatgatc ggagccggct actggggtct gagaagggcc cgcagcgccg acgactacct
     1201 cgtcgcaggg cgacgtctcg gtcccttcat gtacgtcggg actctgagcg ccgtggtgct
     1261 cggcggtgcc tcgaccatcg gcggcgtagc cttgggctac gagaacggca tctccggcat
     1321 gtggctcgtc ttctggatcg gcatgggcgt catagccctc ggcatcctca tgtccacccg
     1381 gctctcgcgt ctcggcgtct acacagtctc cgagatgctc gagaaccgct acggggaggc
     1441 ctcgcgcctg atcagcgcga tcatcatagc tgcctacgcc ctcatgatcg ccgtaacctc
     1501 cactatcgca ataggctccg tcttcaacgt cgtactccct ctctcgccaa gcgtcgcgat
     1561 actcgtcgcc ggcggcatcg tcgtcgccta ctccgtggcg ggagggatgt ggtccatcac
     1621 cctcacggac ttcttgcagt tcctgatcat gaccgtcggc atcttcttct tgttgttgcc
     1681 gctcacaatc gccgccgccg gcggcttctc cgggatgcag gaagcgttgc ctgcctccta
     1741 cttcgacctc actgctatag ggtggcagac gatcttcacc tacttcctgc tgttcttctt
     1801 cgggctgatg atcgggcagg acatctggca gcgggtgttc accgcgcgca gccccgagat
     1861 cgcgcgctgg ggcagcgtcg ccgcaggcgt ctactgcctg ctctacgctc tcgccggtgc
     1921 cctcgtcggg accgcggcac gcgtcatcct gcccgacctc tccaatccgg acaacgcttt
     1981 cgcccgcgta gcgagcgagg ccctaccggc ggggatcacc ggcctggtcc tcgccgccgc
     2041 gctcgcggcc gtgatgtcaa ccgccagcgc cggcctcctc gcctcctcta ccctcctcgc
     2101 caacgacgtc tacgcgggct ttatcgcccg tggcgagcac aacagggtgc tgataaaccg
     2161 cgtgaccgcc ctgctcgtcg gcatcgtagt cctcgtcatc tcgctgatcg tcagcgacgt
     2221 gatcggggct ctgacggtag cctacgacct cctgagcggc gcgctgttcg tgcccatagt
     2281 gggagccctg ttctggaggc gcgcgaccgc agcgggcgcc ctggcttcca tggccgcggg
     2341 cagcgctgtc gtggtgttct tcatggtcaa agatggcctg ttcgcgaaca ccccgatcct
     2401 ctacggtctc gcggcgagcc tcgtcgtctt cgtggtcgtg agcctgctca cgccaaagcc
     2461 ctccgaagag agcatgaggg cttgggaaag caagctcagc gggtccggca ccccgccgta
     2521 gtggaaggta tactggaatg cattagaccg acgggaagag gtgccgaaga tgcttgagtc
     2581 gacgaggatc aagaccgaga taccgggccc gaagagccgg gctttgatgg agaggcggca
     2641 ccgcgctgtc tcgtcgggcg tgggtatcgc gacgccgatg ttcgccaagg aagcgaaggg
     2701 cgccctgttg accgacgttg acggcaacac gttcattgac ttcggcggcg gcatcggggt
     2761 tatgaacgtc gggcacacga acgcgcgggt tgccgaggcg gtcagggagc aggcagagag
     2821 gctcacccac tcctgctttt acgtcacgga gtacgagcag tacatagagc tcgccgagag
     2881 gctcaacgct cttatccccg gggactccga gaagcgctc
//
LOCUS       DASTUV010000530         4437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_17473, whole genome shotgun sequence.
ACCESSION   DASTUV010000530 DASTUV010000000
VERSION     DASTUV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17473"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..473
                     /locus_tag="VFT03_10715"
     CDS             84..473
                     /locus_tag="VFT03_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848660.1"
                     /translation="MGERRTSMIERMVRRVEEHVEEWRKRDATRHAEAEANRDELWAA
                     AAERERLLAEVIGEEEAHRASVEEVMKEHRVVFVVHSEEVAQTLQTFADEKDYLVKAI
                     PSTRGNYAKEMGIKGSWLVFEKSEWRE"
     gene            complement(509..655)
                     /locus_tag="VFT03_10720"
     CDS             complement(509..655)
                     /locus_tag="VFT03_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848661.1"
                     /translation="MNDISEKALKTIEETFGIRFVWHVDGLSATTDIELVRLAGRSWM
                     PQQP"
     gene            complement(687..1286)
                     /locus_tag="VFT03_10725"
     CDS             complement(687..1286)
                     /locus_tag="VFT03_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848662.1"
                     /translation="MTIHLEVFLAVGYAIFLLGVAIGLDLLARHSHARSERYRTAGFT
                     YHPSHDAWICPEDQLLVRSEVDQERRLVRYRGRPQICNHCPAKADCTDSDDGREVVRA
                     MDPWPHSEAGRFHRGISLAVALIAGLILAAEAVRNHAALELAVLGAILFVVALAAWHL
                     HAAFRATPANFPAPRLTPSSKTQINPVQAGYRKSKRVER"
     gene            complement(1290..3596)
                     /locus_tag="VFT03_10730"
     CDS             complement(1290..3596)
                     /locus_tag="VFT03_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU4848663.1"
                     /translation="MTAGSGRRGLLVSKGWVQAVVLVVLFGFFVLGLLAYRAYSGQPP
                     IPDRVVDPGGEVVFTGEDVRAGQGVFLRNGLMQYGSIFGHGAYLGPDFTADYLRRSAL
                     FVRESYGGEDSDRSATLTVSDFKENRYDPGTRTLEFTAPQAAAFEELRGHYGRYFGEP
                     TTKYGLRPEAISDPEEIRQLTAYFAWSAWAASALRPGTDYTYTNNWPPEPLVENEPTA
                     NVVVWSVLSLIALLGGIGLLFAAFGRWNFLGWRGREQATLSFRTPGDVALTPAQRACA
                     WFFLVMAALFLIQTFVGAASQHYRADLASFFGVDLAQVLPYNLMRTWHVQLAIFWVAT
                     SFLAAGIFLAPMIAGREPRGQHLLAFGLLGALAIVVFGSLIGEWAGIFGFTGDLWAWF
                     GNQGFEYRDLGRFWQVLLSIGLFLWVAMLFRAMRGRLRREHMGNMPWLFFFAALAIPA
                     FYAVGLLARPESHFTTADFWRFWVVHLWVEDFLELFTTVMVAYIFVLLGVVREKVALT
                     VIYLDILLYSAGGVIGTMHHLYFSGTPAEHMALGAFFSAAEVIPLTFLTVEAWSFLQL
                     GARQESRSRTPFPHRWAVMFLVAVGFWNFLGAGIFGFLINLPVISYYQIGTALTANHG
                     HAAMMGVYGMLAAGLALFCLRYLIPEERWSERAAKISFWSLNIGLAWMCFATLFPLGI
                     LQLYQSVSVGYYEARTLEFLTNETNALLEWIRLPGDVVFIVGGVLPLLYLCYLGVRYT
                     VPRIVMEEPEDVLFTEITEPEAAGPRER"
     gene            complement(3713..4144)
                     /locus_tag="VFT03_10735"
     CDS             complement(3713..4144)
                     /locus_tag="VFT03_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU4848664.1"
                     /translation="MQLELSSEGRYALRALVYLAWAGERVTADRISTEAHIPRRLLAR
                     ILAELSHAGLVESEQGRSGGSRLARPAGKITLREAVEAIEGPFGVTRCIMEDRACGEG
                     APCALHEAWKEGQRAILEYLGTQTIEEFVSRTASDKLPPRA"
BASE COUNT          919 a         1367 c         1397 g          754 t
ORIGIN      
        1 acgctgcctc tcctcgcagc atcatacagg tcgatggcgg gcaaaatgtt gtcacgaact
       61 gcaaattaga agaggaggta ggaatgggcg aacgtcgcac atccatgatc gaaaggatgg
      121 tacgccgggt cgaggagcac gtcgaggagt ggcgcaagcg tgatgccacc cgccatgccg
      181 aagcggaggc caacagggac gagctgtggg ccgcggcggc ggagagggag agactcctgg
      241 ccgaggtcat aggcgaagag gaagctcacc gagcttccgt agaggaggtc atgaaggagc
      301 accgcgtggt cttcgtggtg cattccgagg aggttgccca gacgcttcag acctttgccg
      361 atgaaaagga ttacctggtc aaagcgatcc ccagcacgag gggaaactac gccaaggaga
      421 tgggtatcaa gggatcctgg ctcgtcttcg aaaagtcgga atggagagag taaatgcgtg
      481 tgtctgtccg ctgcctgatc ggtctctttt acggctgctg aggcatccag gagcggccgg
      541 ccagtcgcac caactcgatg tccgtagtgg cggacaggcc atccacgtgc cacacgaagc
      601 ggattccgaa ggtttcttcg atggttttca gggccttctc ggagatgtcg ttcataagac
      661 gcccttctac ccttcgtgcg ctacgttcaa cgctccaccc tcttgctctt cctgtagcct
      721 gcctgaacgg gatttatctg ggttttagac gatggggtga gtcgcggagc cgggaagttg
      781 gccggggtgg ctcggaaggc agcgtgcagg tgccacgctg ccagagcgac tacgaagagt
      841 atggctccga ggacggcaag ctcgagcgcg gcgtggttgc ggaccgcctc ggcagcgagg
      901 atcaggcccg ctatgagcgc caccgcgagc gagattcccc ggtggaagcg gcccgcttcg
      961 gagtggggcc aggggtccat ggcgcgcacc acctcgcgtc catcgtcgga gtcggtacag
     1021 tcggccttgg ccgggcagtg gttacagatc tggggcctgc cccggtagcg taccagccgc
     1081 cgctcctggt ccacctcgct cctgacgagg agctggtcct cggggcagat ccaggcgtcg
     1141 tgcgagggat ggtaggtgaa ccctgctgta cggtagcgct ccgagcgagc gtgagagtgg
     1201 cgggctagga ggtcgagccc gatggcgact ccgagcaaaa agatcgcgta gcctaccgcg
     1261 agaaagacct cgaggtggat ggtcattacc tatcgctccc tcggcccggc cgcttcgggc
     1321 tcggtgatct cggtgaacag tacgtcctca ggctcctcca tgactatccg cgggaccgta
     1381 tagcgcacgc cgaggtagca gaggtacagg agcggcagaa cgccgccgac gatgaagacg
     1441 acgtcgccgg gcaggcgaat ccactcgagg agggcgttcg tttcgttagt caggaactcg
     1501 agcgtgcggg cctcgtagta gccgacgctc accgactggt agagctgcaa gataccaagg
     1561 gggaatagcg tcgcgaagca catccaggca agccctatgt tgagcgacca gaagcttatc
     1621 ttcgctgccc gctccgacca gcgctcctct gggatcaggt agcgcaggca gaagagcgcc
     1681 agacccgccg cgagcatccc gtagacaccc atcatggcgg cgtggccatg attggcggtc
     1741 aaagcggtgc cgatctggta ataggagatt acggggaggt ttatgaggaa gccgaagatc
     1801 ccggcgccga ggaagttcca gaaccctacc gcgaccagga acatgacggc ccagcgatgg
     1861 gggaacggcg tgcgcgacct cgactcctgc cgcgccccga gctgcaagaa gctccaggct
     1921 tcgacggtca ggaaggtcaa ggggatcacc tccgctgccg agaagaacgc gcccagagcc
     1981 atgtgctcgg ctggcgtgcc cgagaagtag aggtggtgca tcgtcccgat cacacccccc
     2041 gccgagtaga gcagtatgtc cagatatatg acggtcaggg ccaccttctc gcgcacgacc
     2101 ccgagcagaa caaatatgta ggcgaccatg acggtcgtga agagctcgag gaagtcctcg
     2161 acccagaggt gtactaccca gaaccgccag aagtcggccg ttgtgaagtg cgactcgggc
     2221 cgggcgagca gcccgacggc gtagaacgcc gggatcgcaa gggcggcgaa gaagaacaac
     2281 cacggcatgt tgcccatgtg ctcgcgccgc aaccgcccgc gcatcgccct gaacagcatc
     2341 gcgacccaca agaacaggcc tatcgagagc agtacctgcc agaagcgacc gaggtcgcgg
     2401 tactcgaagc cctggttgcc gaaccaggcc cacaggtcgc ccgtgaagcc gaagatcccg
     2461 gcccactccc cgatgaggct cccgaagaca actatggcca gggcgccgag cagcccgaag
     2521 gcgagaaggt gctgcccccg cggctctctg cccgcgatca tcggcgccag gaatatcccc
     2581 gccgccagaa acgaggtcgc cacccagaag atagcaagct gcacgtgcca ggtgcgcatg
     2641 aggttgtaag gcagaacctg tgcgaggtcc acgccgaaga agctcgccag gtcagcccgg
     2701 tagtgctggg acgccgcgcc cacgaacgtc tggatcaaaa acaacgcggc catgaccagg
     2761 aagaaccacg cgcacgctct ctgggccggc gtgagagcca cgtcgccagg ggtgcgaaac
     2821 gatagcgtcg cctgctcgcg cccacgccag ccgaggaagt tccaccggcc gaaggccgca
     2881 aagaggagcc cgatgccgcc aaggagcgcg atcagggaca gcacgctcca caccaccacg
     2941 ttggcggtcg gctcgttctc cacgaggggc tccggcggcc agttgttggt gtacgtatag
     3001 tccgtcccag gccgcaaggc agacgcggcc cacgcactcc aggcgaagta cgccgtgagc
     3061 tgcctgatct cctcgggatc gcttatagcc tcgggcctga gcccatactt cgtagtcggc
     3121 tccccgaagt accgcccgta atgtcccctg agctcctcga aggccgcagc ctgcggcgcg
     3181 gtgaactcga gggttcgcgt cccgggatcg tagcggttct ccttgaagtc ggagacggtc
     3241 agcgtcgctg acctgtctga gtcctctcct ccgtaactct cacgcacgaa gagggcggat
     3301 cgccgcagat aatcggcggt gaagtcgggt ccgaggtagg cgccgtgccc gaagatggaa
     3361 ccgtactgca tcagaccgtt gcgcaggaac accccctgcc cggccctcac gtcctctccg
     3421 gtgaagacga cctcgccccc aggatccacg acccgatcgg gtatcggcgg ctgccccgaa
     3481 tacgcccgat atgccagaag cccgagcacg aagaagccga acaggacgac cagcactaca
     3541 gcctggaccc agcccttcga gacaagcaat ccgcgtcgcc ccgatcctgc tgtcaccaac
     3601 cacctccgga tgatcccggt cagggcagat tgcaccaccc ctaactatta cctaattagt
     3661 aatataactt ttgggttgac cattgcgcaa gcggttcggc gtaacgccta cgctatgccc
     3721 ttggagggag cttatcggag gcagtccgtg agacaaactc ttcgatggtc tgtgtaccga
     3781 ggtactcgag gatagctcgc tggccctctt tccaggcttc gtgcagggcg cagggagcgc
     3841 cttctccgca ggcgcgatcc tccataatgc agcgagtgac accgaagggt ccttctatgg
     3901 cttctacggc ttcccgaagc gttatcttgc ccgcaggacg cgccagccgg gagcctccac
     3961 tgcggccttg ttcgctctcc accagcccgg cgtgggagag ctcggccagg atgcgtgcca
     4021 gtaggcggcg cgggatgtgg gcttcggtcg agatcctgtc cgcggtcacc cgctcaccag
     4081 cccaggcaag gtagaccaga gccctcaagg cgtaccggcc ttcgctgctc aactcgagtt
     4141 gcacactcgt ctctccttac ccgtttccct tgcattcttg ctcatctccg ttaaggcgga
     4201 caagttgggg aacatctggc gcataagcgt gtcgatctat tcgatcacca gttctgtaat
     4261 agtatttgtg taatactggc gcctctccca aatactacct tcagagcaaa tatattacgt
     4321 tgcaaagatc actcatttgg gggatgcacc cggtaatcaa tgcttgatac ggtagtggcg
     4381 ttatataaca cacggcgggg ctctgaggag cctcgcccta catgagagag gcctcgc
//
LOCUS       DASTUV010000531         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1386100, whole genome shotgun sequence.
ACCESSION   DASTUV010000531 DASTUV010000000
VERSION     DASTUV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386100"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /gene="glgX"
                     /locus_tag="VFT03_10740"
     CDS             <1..1685
                     /gene="glgX"
                     /locus_tag="VFT03_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEU4848665.1"
                     /translation="PRIPWHDTIIYETHVKGFTQRHPNIPPALRGTYAGLASAPAIDH
                     LKRLGATAVELMPVHLFVDDKHLVDRGLRNYWGYNSIGFLAPDMRYSATGSINEFKTM
                     VKRLHSAGLEVILDVVYNHTAEGNHLGPTLSFRGIDNAAYYRLVPDDRRYYMDFTGTG
                     NTLNMMHPRVLQLIMDSLRYWILEMHVDGFRFDLASALARELYEVDQLSSFFTIIHQD
                     PVISQAKLIAEPWDVGEGGYQVGNFPVGWTEWNGKYRDAVRAYWKGEGGRTGELAYRL
                     TGSSDLYERGGRKPHASINFVTAHDGFTLRDLVSFEEKHNEANGEGNTDGENHNLSRN
                     YGVEGPTDDVEIRRLRARQRRNLMATLLLSQGVPMILSGDELGRTQNGNNNAYCQDNE
                     ISWLDWELSTLDRNFLAFVRRVIRLRKEHPAFRRRSFFQGRELRGVKDITWLTPAGRE
                     MTDEEWNSSFARSLGLQMSGLLEGEYDAQGRPEVDDDFLLLFNAHEEEVAFTLPSVPE
                     EARWECFMDTAFSAGLKPDGFFKPGGVYALKPLSMAVLVNDRQHENVDEDAD"
     gene            1709..>3028
                     /locus_tag="VFT03_10745"
     CDS             1709..>3028
                     /locus_tag="VFT03_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU4848666.1"
                     /translation="MWWQRSVVFQVYPRSFQDSDGDGVGDLRGMVSRLSHLEWLGVDA
                     VWVSPFYPSPMADFGYDISDYTDIHPIFGTLTDFDAFVAEAHRRDMKVILDYVPNHTS
                     EEHPWFVESRSSRENPRRDWYIWQDPAHDGGPPNNWQSVFGGGAWEWDEETQQYYYHA
                     YLREQPDLNWRNPEVREAMMDVLRFWLERGVDGFRVDALRQLVKDEHLRDNPSNPDER
                     TRSPYDALLPVYSTDRPEVHEAVREMRSVLEEYGDRLLVGELYLPIDRLVTYYGEDNS
                     GVHLPFNFHLISTPWNAREISTLIDTYEDALPPGGWPTWVLGNHDRSRIASRVGDAQA
                     RVAAMLLLTLRGTPTLYYGDELGMRDVEIPPERVQDPFEKNVPARGLGRDPARTPMQW
                     DASPNSGFCAPDVEPWLPVADDYWTYNASTEREDPESMLNLNRRLLAL"
BASE COUNT          584 a         1041 c          915 g          488 t
ORIGIN      
        1 cgccacgcat cccctggcac gacaccataa tttacgagac ccacgtcaag gggtttacgc
       61 agaggcaccc gaacatccct ccggcgctgc gcggtacata cgccggcctg gcttcggccc
      121 cggccataga ccacctgaaa cggctcgggg caacggcggt cgagctcatg ccggttcacc
      181 tcttcgtgga cgacaagcac ctcgttgaca ggggcttgag gaactactgg ggctacaact
      241 ccatcggctt cctcgcgccc gacatgcgct actcggccac gggcagcatc aacgagttca
      301 agacgatggt caagaggctg cactcggctg gcctcgaggt gatcctcgac gtcgtctaca
      361 accacacggc cgaaggcaac cacctcggac cgacgctctc ttttcggggc atagacaacg
      421 cggcctacta ccggctagtc ccggacgaca ggcgctacta catggacttt acagggacag
      481 gcaacacgct caacatgatg cacccgcgcg tgctgcagct catcatggac tccctgcggt
      541 actggatcct ggagatgcac gtggacgggt tccgcttcga tctggcctcc gccctggccc
      601 gcgagctcta cgaggttgac cagctctcga gcttcttcac gatcatccac caggacccgg
      661 tgatctccca ggccaagctc atcgccgagc cgtgggatgt gggcgaggga ggctaccagg
      721 tcggcaactt cccggtgggc tggacggagt ggaacggcaa gtaccgcgac gccgtccgcg
      781 cctactggaa aggtgagggc ggacgcacgg gcgagctggc ctaccggctc accggctcga
      841 gcgacctcta cgagcgcgga gggcgcaaac cccacgcgag cataaacttc gtcaccgccc
      901 acgacggctt caccctgcgg gacctcgtct ccttcgagga gaagcacaac gaggccaacg
      961 gcgagggaaa caccgacggc gagaaccaca acctgagccg caactacggc gtcgaaggac
     1021 cgaccgacga cgtggagatc cggcgcctca gagcccgcca gagacgcaac ctcatggcaa
     1081 ccctcctgct ctctcaaggc gtgcccatga tcctctcggg cgacgagctg ggccgtaccc
     1141 agaacggaaa caacaacgcc tactgccagg acaacgagat aagctggctg gactgggagc
     1201 tctcgaccct cgacaggaac ttcctggcct tcgtgcggcg cgtgatccgg ctgcgcaagg
     1261 agcatcccgc cttccggcgc cggagcttct tccaggggcg ggagctgagg ggcgtcaagg
     1321 atatcacctg gctcaccccc gccggcagag agatgacaga cgaggaatgg aacagctcct
     1381 tcgcccggtc cctcggcctc cagatgtccg gcctcctcga aggcgaatac gatgcgcagg
     1441 gccgtcccga ggtggacgac gacttcctcc tcctcttcaa cgcccacgag gaagaggttg
     1501 ccttcaccct cccgtccgtc cccgaggagg ctcgctggga gtgcttcatg gacaccgcct
     1561 tctccgccgg tctgaagccg gacggcttct ttaaacccgg cggcgtgtat gctctgaagc
     1621 ctctctccat ggccgttctc gtgaacgacc gccagcacga gaacgtcgat gaggacgcgg
     1681 attgagtccg ggggaccggt aggtattcat gtggtggcag cgcagcgtcg tcttccaggt
     1741 ctacccgcgc tccttccagg actccgacgg agacggcgtc ggcgacttga gagggatggt
     1801 tagccggctg agccatctcg aatggctggg cgtggacgct gtctgggtct cacccttcta
     1861 cccttccccg atggccgatt tcggctacga catcagcgat tacacggaca tacacccgat
     1921 attcgggacg ctcacggact tcgacgcctt cgtcgccgag gcgcaccggc gcgacatgaa
     1981 ggtgatcctg gactacgtcc cgaaccacac ctcggaggag cacccctggt tcgtcgagtc
     2041 gcgctcctcg cgcgagaacc caagacgcga ctggtatatt tggcaggacc ccgctcacga
     2101 tggaggaccc cccaacaact ggcagagcgt cttcgggggt ggagcctggg agtgggatga
     2161 ggagacgcag cagtattact atcacgccta cctcagggag cagcctgacc tcaactggcg
     2221 caaccccgag gtcagggagg cgatgatgga tgttctgcgc ttctggctgg agcggggcgt
     2281 agacgggttc agggtggacg ccctcaggca gcttgtgaaa gatgagcatc tgcgcgacaa
     2341 cccgtccaac cccgacgaac gtacgaggtc accgtacgat gcgctgctcc ctgtctactc
     2401 caccgaccgg cccgaggtac acgaggccgt ccgcgagatg cgcagcgtgc tcgaagagta
     2461 cggcgaccgg ctcttggtcg gcgagctcta cctgccgata gaccggctcg tgacctacta
     2521 cggcgaggac aactcgggcg tccatctgcc cttcaacttc catctcatct ccaccccctg
     2581 gaacgcgcgg gagatctcca ccctcataga tacctacgag gacgcgctcc cgccaggcgg
     2641 ctggcctacc tgggtgctcg gcaaccacga ccgctcccgc atcgcgagcc gcgtgggcga
     2701 tgcgcaggcg cgcgtagcgg cgatgctcct cctgacgctg cgcggcacac ccaccctcta
     2761 ctacggggac gagctcggga tgcgcgacgt cgagatacca cccgagcggg tccaggaccc
     2821 cttcgagaag aacgtccccg cccggggcct gggccgcgac cccgcccgca ctccgatgca
     2881 atgggacgcg agccctaact ccggcttctg cgcgcccgac gtcgagccct ggctacccgt
     2941 cgccgacgac tactggacat acaacgcctc taccgagcgc gaagatccgg aatccatgct
     3001 gaacctcaac cgccggctcc tcgcgctg
//
LOCUS       DASTUV010000532         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1053911, whole genome shotgun sequence.
ACCESSION   DASTUV010000532 DASTUV010000000
VERSION     DASTUV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053911"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..422
                     /locus_tag="VFT03_10750"
     CDS             180..422
                     /locus_tag="VFT03_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848667.1"
                     /translation="MSFLSGSRPGRWFVWIIYRALNKPLPVPHAAEWMMIPEHSVDQV
                     LETGALRSLYPQDVLEAARRMDRQRREHDERHANKS"
     gene            457..1743
                     /locus_tag="VFT03_10755"
     CDS             457..1743
                     /locus_tag="VFT03_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU4848668.1"
                     /translation="MDLFDESGSESLSRRAPLAERLRPRTLDEVVGQPHLTGEDGPLR
                     AAIERGKIGSLVLWGPPGTGKTTLARVLAGTVEEEFVPLSAVTSGVKDLRAAMDGARE
                     RLKYEDRGTLVFVDEVHRFNKAQQDALLPALEEGLVDFIGATTENPSFEVTAPLLSRS
                     RVLRLRPLSEGELGELLERGAEELGVEVSPEAREYLLRLSGGDGRRLLNALEVAAVGA
                     DSVEVGNVERAVGQRSLRYGREEHYDVISAFIKSVRGGDPDAALHYLARMLDAGEDPV
                     FVARRLVILASEDVGNADPHGLPLAVAAAQAVQLIGMPEGRIPLAQATTYLASAPKSN
                     ASYAAIGRALEDVRQGPVAEVPLHLRNAATGLMKEEGYGAGYRYAHDDEPEGMNDRYL
                     PEELAGRVYYKPAKSGAEAEIRARIERWREARDERE"
     gene            complement(1911..>2574)
                     /locus_tag="VFT03_10760"
     CDS             complement(1911..>2574)
                     /locus_tag="VFT03_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEU4848669.1"
                     /translation="EGGHRLYTAEDVVRLQRIKSLRALGLSLREIGEYLDGADVPPGR
                     LIELHLARLKERIELQRRLCDRLEQVASRLRSGEEISSERFVELVMEVIEMSERLDKY
                     YSPEQLEYLEARRREVGEERIRESQAEWAELMENVRAEMEAGTDPSNERVQALAARWM
                     ALVEEFTGGDPGIERSLGNMWRQEENIHGIDTGEAREMGRYITAAMAASQEPPDDDAP
                     SS"
BASE COUNT          425 a          864 c          851 g          434 t
ORIGIN      
        1 gcagctccga gagctccggc ggcagccctt cgagggcttt ctccaccagc tcgtagaagt
       61 ccagggtctc actgctcacg ggcgtcattg tacttcagag ctcactagcg tttcgcctgg
      121 cagcgctcct ctaagctaga ataagctacc gccaggcgga ctgaaaaagg gtgttgcatt
      181 tgagctttct atccggttcg cgtccgggac gatggtttgt ctggataatt taccgcgcgc
      241 tcaacaagcc gctgccggtg ccgcatgcgg cggagtggat gatgatcccc gagcactctg
      301 tggatcaggt tctcgagacc ggcgccctcc ggagcctcta cccgcaagat gtcctcgaag
      361 ccgcccgccg catggaccgc cagcgccgcg agcacgacga gcggcacgcc aacaaatcct
      421 gagggtgcca gaccccctcg ccggtatact agtactgtgg acctcttcga cgaatccggc
      481 tccgagagcc tctcccgccg cgcccccctc gccgagcgtc tgcgcccgag gacgctggac
      541 gaggtagtcg ggcagccgca cctgacaggc gaggacggac cgctccgcgc agcgatcgag
      601 cggggcaaga tcggctccct cgtcctctgg gggccgccgg ggacgggcaa gacgacgctg
      661 gcgcgggtgc tcgccggcac ggtagaggag gagttcgtgc ccctcagcgc cgtgacgagc
      721 ggggtgaagg acctgcgggc ggccatggac ggcgcccgcg agcgcctgaa gtatgaggac
      781 cgggggacgc tcgtgtttgt ggacgaggtc caccgcttca acaaggcgca gcaggacgcc
      841 cttttgccgg cgctcgagga ggggttggtg gacttcatcg gggcgacgac cgagaacccc
      901 tccttcgagg ttacggcccc gttgctctcg cgctcccgcg tgctcaggct gcgcccgctc
      961 tccgaagggg aactcggcga gctgctcgag cgtggggcgg aggagctggg tgtggaggtc
     1021 tcgcccgagg cgagggagta cctgctgagg ctgtcgggtg gtgacgggag gcggttgctc
     1081 aacgcgctgg aggtcgctgc ggtcggcgcg gatagcgtcg aggtggggaa cgtcgagcgg
     1141 gcggtggggc agaggtcgtt gcgctacggg cgcgaagagc actacgacgt catcagcgcc
     1201 ttcataaaga gcgtgcgcgg cggcgaccca gacgcggcgc tgcactatct ggcccggatg
     1261 ctcgatgccg gggaggatcc cgtcttcgtg gcccggcggc tcgtgatcct cgcctccgag
     1321 gacgtcggca acgccgaccc gcacggtctt cccctcgcgg tggccgcagc ccaggctgtt
     1381 cagctcattg gcatgcccga gggccgcatc ccgctcgccc aggcgacgac gtacctcgcg
     1441 tccgccccga agtccaacgc ctcctacgcc gctataggac gagccttgga ggacgtgcgg
     1501 cagggtcccg tggcagaggt ccccttgcac ctgcgaaacg cagccacggg tctaatgaag
     1561 gaagaaggat acggggcggg gtaccgctac gcgcacgacg acgagccgga ggggatgaac
     1621 gaccgctatc tgccggagga gctcgcaggc cgggtctact acaagccggc aaagagcggc
     1681 gctgaagcgg agatccgagc ccgaatcgag cgctggcgcg aggcaaggga tgagcgggag
     1741 tgacgtccgg cggacgccgg gctggctaca gcgtttctcg aaacgatcga tctcgcagcc
     1801 cgtcgacgtt gcgtgcggaa atgcgcctca gccgactagc tcggcgagtc catcgacctg
     1861 aatctcatga ccagaggact tcgccgggta cccgaacacc ctggtcgttg ctacgagctg
     1921 ggggcatcat cgtccggcgg ttcctgcgac gcggccatgg ctgcggtgat gtaccgtccc
     1981 atttcccgcg cctcgccggt gtctataccg tggatgttct cctcctgccg ccacatgttg
     2041 ccgagggagc gctcgatccc aggatctccg ccggtgaact cctcgacgag cgccatccag
     2101 cgcgcggcga gggcctgcac ccgctcgttt gacgggtctg tgccagcctc catctcggcg
     2161 cggacgttct ccatgagctc ggcccactcg gcctgcgact cgcggatcct ctcctctccg
     2221 acctcgcgcc tcctcgcttc cagatactcc aattgctccg ggctgtagta cttgtcaagc
     2281 ctctcggaca tctcgatcac ctccatgacc aactctacga accgctcgga agagatctcc
     2341 tcccccgagc gcaaccggga tgcgacctgc tccaggcggt cgcagagcct cctctgcagc
     2401 tcgatcctct cctttagccg cgccaggtga agctcgatca agcgccccgg aggaacgtca
     2461 gctccgtcca gatactcccc gatctcccgc aggctgagcc cgagagccct gagcgatttg
     2521 atccgctgta gccgaacgac gtcttcggcc gtataaagcc ggtgtccccc ctcg
//
LOCUS       DASTUV010000533         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_410568, whole genome shotgun sequence.
ACCESSION   DASTUV010000533 DASTUV010000000
VERSION     DASTUV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410568"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFT03_10765"
     CDS             <1..834
                     /locus_tag="VFT03_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4848670.1"
                     /translation="GSARMLASRLEKAFPQIRLIQNTSLFDLEDFDPDGFDLVVSTIP
                     LPIPSGSYVQVHPLLSEDQVESIRDHLREKMIRDPLRDKSIDARLAERASSESLEVFG
                     GGQTKFDQMAEATQVIAELLEDAFLARHEARGSVPEAVRLMCESLAGRGLISEPERLE
                     ADLLARMELGGIGIPGTTLALFHARGDTVVRPSFSVHDLDEPLELEGMDGAMMRVRRS
                     LLMVAPPELSPVALEAISEISVAMVERPAEREVFEEGSEVQVIALLNGIFARYLQSKL
                     A"
     gene            848..1285
                     /locus_tag="VFT03_10770"
     CDS             848..1285
                     /locus_tag="VFT03_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4848671.1"
                     /translation="MSEILAPETIELKASLDSQDEAIRRAGQLLVDNGHVDDQYIDSM
                     FEREKSVSTFMGNGVAIPHGTGDSKQWVKRSGLSVITVPEGVEYGDGNVATLIVGIAG
                     KGDEHLEILSKIALVVAEEENVERLVNAETKEELLAIFEEVKV"
     gene            1282..1785
                     /locus_tag="VFT03_10775"
     CDS             1282..1785
                     /locus_tag="VFT03_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848672.1"
                     /translation="MKSVHFGAGNIGRGFIGQLLHESGYDITFVDIQDDLVDALKRDG
                     RYDVILADEREERIPVDRVTALHSINEAAEVTERLAEADLITTAVGPSVLPVLAPAIA
                     KGLVERERRGGVPVNVIACENMVGASQALRDFVMENVPDEDAEAVEEIAGFPNAAVDR
                     IVPERQA"
     gene            1782..2423
                     /locus_tag="VFT03_10780"
     CDS             1782..2423
                     /locus_tag="VFT03_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848673.1"
                     /translation="MNIDVLVEPFYEWVVDASQIKGKRPGVSGITYVEDPLPYIERKL
                     LTVNAGHSAIAYLGYVRGKPTIHAALEDDEVRETASKTLEETSLLLIKEYGFEPEELR
                     EYRRKVLARFSNPYISDDVTRVARAPIRKLGRNERFVSPALRLLDMGHMPVHLATVIG
                     AVLRYDYPEDAEAEELQETIRAAGERSALARYAGIGEDHPLVDLVVERMDHVR"
     gene            2539..>2784
                     /locus_tag="VFT03_10785"
     CDS             2539..>2784
                     /locus_tag="VFT03_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU4848674.1"
                     /translation="MVEKETTVGPKEGLHARPAAEFVQKAKGFSSAIVVEKDGREANA
                     KSPLKLMTLAAKHGDKITIRAEGDDAQEAVDSLVAQIS"
BASE COUNT          587 a          798 c          936 g          463 t
ORIGIN      
        1 ggctccgcca gaatgctcgc ctccaggctg gagaaagcgt tcccccagat tcggctaata
       61 caaaacacct cgttgttcga tctcgaggac ttcgacccgg acgggttcga ccttgtcgta
      121 tccacgatcc ccctgccgat ccccagcgga tcttacgtgc aggtccatcc gctcctctcc
      181 gaagaccagg tggagagcat aagggaccac cttagagaga aaatgataag agatcccctc
      241 agagacaaga gcatcgacgc tcgcctcgcg gagcgcgcgt cctccgagtc cctggaggtc
      301 tttggcgggg gacagacgaa gttcgaccag atggccgagg cgacgcaggt aattgcggag
      361 ctactggagg acgccttcct agcgcggcac gaggcccgtg gctccgtgcc cgaagcggtg
      421 cgcctgatgt gcgagtcgct cgccgggcgg ggcctgatct ccgaacccga acgcctggag
      481 gctgatctgc tcgcgcgcat ggagcttggc ggaatcggga taccgggtac cacgctggcc
      541 ctgttccatg cccgcggcga caccgtcgtc agaccttcct tctcggtaca cgacctcgac
      601 gagcctctgg aactggaagg tatggacggt gcgatgatgc gggtccgccg gagcttgttg
      661 atggtcgcgc cgccggagct ctccccggta gccctcgaag caattagcga gatatcggtc
      721 gcgatggtcg agcggcccgc ggagcgcgag gtcttcgaag agggctcgga ggtccaggtc
      781 atcgcgttgc tcaatggcat atttgcccgg tatttgcaga gtaaactagc gtgaaaggag
      841 ctgggaagtg tccgagatac tggcgccgga gaccatcgaa ctgaaggcca gcctggatag
      901 ccaggacgag gcgatccggc gggcaggcca gctgctcgtc gataacggcc acgtggatga
      961 ccagtacata gactccatgt tcgagcggga gaagtcggtc tcgacgttca tgggcaacgg
     1021 cgtggcgatc ccccacggca cgggggactc aaagcagtgg gtcaagaggt cagggctctc
     1081 cgtcatcacc gttcccgaag gtgtggagta cggtgacggg aacgtggcga ccctcatcgt
     1141 cgggatcgct ggcaagggcg acgagcacct ggagatcctc tcgaagatcg cactcgtcgt
     1201 ggccgaggag gagaacgtcg aacggctcgt gaacgccgag actaaagagg aactgcttgc
     1261 gatcttcgaa gaggtaaagg tttgaagagc gtccacttcg gagccggcaa catcggccgc
     1321 ggctttatag gccagctgct gcacgagtcc ggctacgaca tcaccttcgt ggacatccag
     1381 gatgacctgg ttgacgccct gaagagggat gggcgctacg acgtgatact ggcagacgag
     1441 agggaggagc ggatccccgt ggacagggtc accgcgctcc actccataaa cgaagcagcc
     1501 gaggttaccg agcgtctggc ggaggcggac ctcatcacca cggcggtcgg accgtcagtc
     1561 ttgcctgttc tggcccccgc gattgcgaag gggctcgttg agcgcgagcg tagaggagga
     1621 gtgccggtca acgtgatcgc ctgcgagaac atggtcgggg cgagccaggc gctgcgggac
     1681 ttcgtgatgg agaatgtccc ggacgaggac gcggaggcgg tcgaggagat agccggcttc
     1741 ccgaacgcag cggttgaccg catcgtaccg gagcgccagg catgaacatc gacgtcctgg
     1801 tcgagccttt ctacgaatgg gtagtcgatg cctcgcagat aaaggggaag cgtccgggtg
     1861 tctcgggcat aacttatgtc gaggacccat tgccctacat cgagaggaaa ctgctcacgg
     1921 tgaacgcggg ccactccgca atagcctacc tgggctacgt ccgcggcaag ccgacgatcc
     1981 acgccgccct cgaagacgac gaagtccgcg agacggcgtc aaagaccctg gaggagacca
     2041 gtctgctgct catcaaggag tacggcttcg aaccagaaga gctccgcgaa taccggcgga
     2101 aggtcctcgc ccgcttcagc aacccataca tctccgacga cgtaacccgg gttgcgcgtg
     2161 cccccatccg gaaactcggc cgcaacgagc gctttgtctc acctgccctg cgcctgctcg
     2221 acatggggca catgccggtg cacctggcga cggttatcgg ggcggtgctg cgctacgact
     2281 atcccgagga tgcagaagca gaggagctac aggagacgat acgcgcggca ggcgaacgtt
     2341 cggcgctggc ccggtacgct gggataggag aggaccatcc gctggtagat ctcgtcgtgg
     2401 aacggatgga tcacgttcga tagtagtagt gtagtagtgg gaaaggcgcg tgtgttgggg
     2461 tcgaagcacg aaatgttggc ccgtagagac cgtgcggcct ggagtgaagc aagggaaggg
     2521 acaggaggag gagtgcacat ggtagagaag gagacgacgg ttggaccgaa ggaggggctc
     2581 cacgcccgtc cagcggccga gttcgtgcag aaggccaagg ggttttcttc agccatcgtt
     2641 gtggaaaagg acgggcgcga ggctaacgcc aagagccctc ttaagctgat gaccctggca
     2701 gccaagcacg gcgacaagat aaccatccgg gccgaaggag atgacgccca agaggccgtc
     2761 gactccctgg tcgcgcaaat ctcg
//
LOCUS       DASTUV010000534         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_839815, whole genome shotgun sequence.
ACCESSION   DASTUV010000534 DASTUV010000000
VERSION     DASTUV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839815"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..1424)
                     /locus_tag="VFT03_10790"
     CDS             complement(441..1424)
                     /locus_tag="VFT03_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4848675.1"
                     /translation="MKAVQIEEFGGPEVLKTAEIPTPKAREDEVLIELRASGVNRSDI
                     LTRSGSYHAAGQPPIVPGFEGSGIVREPGPAATGISSGDRVLAFDGRPGFYAEYVAVP
                     TRHVVKIPGTLDWDSAAALPVAPLSAWYCLQRLARLRNGEKVLIWAAASGVGDAAVQI
                     AKHLGATVIATAGTDEKVAWALENGADEGINHARENVLKRTRAIAGEEGVEVVLDTVG
                     GSIFGESLKVVGHGGRVVALANVALEESRIDTRDFYPKNATIYGFQITNLIQRLGYGP
                     RDDLGELADLVVRNKLEIHVDRTFPLGRAADAHRYLEERRNRGKVIIHPGD"
     gene            complement(1523..>2018)
                     /locus_tag="VFT03_10795"
     CDS             complement(1523..>2018)
                     /locus_tag="VFT03_10795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU4848676.1"
                     /translation="DDPMPGAAASREMGGLPFLWAILAGEQPPPPIGELMGFEPLEVE
                     EGRALFVCTPAEYHYNPIGAVHGGLACTLLDSAMGCAVHTMLPAGVGYTTVELKVSFL
                     RPITLKTGRLLCEGTIIHLGSRIATAEARLLDTSGKLYGHATTTCMILRNLAPEQSKP
                     SVGE"
BASE COUNT          347 a          672 c          585 g          414 t
ORIGIN      
        1 ctccacgatc tcgagcacct ccggcccgtc cttcctcgtc aggagcaccc gcttttgctt
       61 catccccgcg tcttttccgt ctctcactct tgctcctccc caaacggtcc tgctttgttt
      121 cgcccggcgc tcggacgccc cacggggaac tactcccctc ggcgacgggc atactctaca
      181 gcgcgtcgcc ctcccggcgc atcgctcttc ttcgtctccg tcccgcatcg tacggcaaca
      241 cgggagtcgc gtggttgcaa gggagccaac accgccttct ggaaggcata cgagagccgg
      301 gcggtccgcc ggagggtctg cccgcccgtg agggggtgtt tctgccaccg acgtgacgga
      361 tacgtgtagc ggtggccgtg gctaggtttg ctcgcatctg gcacgttcag aatatcctac
      421 ccacctcctc cgacttcgct ctaatcccct gggtggataa taaccttgcc tcggttgcgg
      481 cgttcctcca ggtagcggtg ggcatcggcc gctctcccta ggggaaaggt ccggtccacg
      541 tggatctcga gcttattccg gaccacgagg tcggccaact ctccgaggtc gtcacgtgga
      601 ccgtagccaa gtcgttgtat gaggttggtg atctgaaacc cgtagatcgt cgcgttcttc
      661 gggtagaagt cgcgagtgtc gatccgactc tcctcaagcg caacgttcgc tagagctacc
      721 acacgtccgc catgaccgac gactttaagg ctctcgccaa agatcgatcc ccccacagtg
      781 tcgagtacca cctcaacgcc ctcttcccca gcaatggctc tcgttctctt cagaacgttc
      841 tctcgggcgt ggttgatccc ttcgtccgca ccgttctcta aagcccaggc aaccttctcg
      901 tcggtgccgg cggtagcgat aaccgtggcg cccagatgct tagcgatctg taccgccgcg
      961 tcgccaacgc cgctggcagc cgcccagatg aggacttttt cgccgttccg aagccgggcg
     1021 agacgctgca gacagtacca ggccgagaga ggagcgacgg ggagagccgc cgcggagtcc
     1081 cagtctagag tgcctggtat cttcacaacg tggcgtgtcg gaactgcgac gtactcagca
     1141 tagaagccag gcctgccgtc gaaggcgaga acccggtcac cggagcttat gcctgttgcc
     1201 gccgggccag gctctcggac aataccgctt ccctcaaagc ccggcactat cgggggttgc
     1261 cccgcggcgt ggtagctgcc actacgagtg agtatgtcgg atcgatttac acctgaggcc
     1321 cgaagctcga tcaggacttc gtcctccctt gccttgggag taggaatctc cgccgttttc
     1381 agtacctctg gaccaccgaa ttcctcgatc tgaacggctt tcacgtcgtg ttcctttccg
     1441 cagtctattt cggcactctg tactgcgccg ctaggaaacc tctacaggtc gtctgccgac
     1501 tacccgcaca cgctcatcat cctcactcgc caacggaagg ctttgactgt tcgggtgcta
     1561 ggttgcgcaa gatcatgcag gtagtggtgg cgtgaccata gagcttgccg cttgtgtcga
     1621 ggagcctcgc ctcggcggtg gcgatgcggc tgccgaggtg gatgatggta ccctcgcaca
     1681 gcagccgccc ggtcttcaac gtgatcggac ggaggaagct taccttcaac tcgaccgtag
     1741 tgtacccgac gccggcaggg agcattgtgt gcacggcgca gcccatcgcg gagtcgagga
     1801 gagtgcacgc caagcccccg tgcaccgcgc cgatcgggtt gtagtggtac tcggcgggcg
     1861 tacatacgaa caaggcgcgg ccctcctcca cctccaacgg ctcgaagccc atgagctctc
     1921 ctatgggagg cgggggctgc tcgccagcga gaatggccca caggaacggt agaccgccca
     1981 tctccctgga agccgctgcc ccgggcatgg gatcatcc
//
LOCUS       DASTUV010000535         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_124889, whole genome shotgun sequence.
ACCESSION   DASTUV010000535 DASTUV010000000
VERSION     DASTUV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124889"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFT03_10800"
     CDS             <1..359
                     /locus_tag="VFT03_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU4848677.1"
                     /translation="GMRVAVVTSKRRSSVEMALKIFPGLGKVVDQWVTMEDTTEHKPR
                     PEPLLKGLEMLGNVPREQAAYVGDSPFDVTAAKAAGVESVAVSWGAFSEDALRAAEPD
                     QLVPDLDAAVDVLLSA"
     gene            648..950
                     /locus_tag="VFT03_10805"
     CDS             648..950
                     /locus_tag="VFT03_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848678.1"
                     /translation="MAYKDELRVPAGAPVTFAYGGRKLDSIAVVAYRIGPGNQPEQMG
                     RKILLIPAGKGTELPARRSGNRAQISAELPAGRYVVDVFATMPGGDASYGFLIAVE"
     gene            complement(934..2061)
                     /locus_tag="VFT03_10810"
     CDS             complement(934..2061)
                     /locus_tag="VFT03_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU4848679.1"
                     /translation="MFPALSSNDFVYLNSGSSGPPSYGVIEAMRAADALCTGAAYLEG
                     AGLYARQADIRERARESAAQLIGAAPQDVALTQNTTHGMNLGTASIDWREGDEVISAT
                     TEHPGCLVPLHNLRARSGVKVQLVSPPVTPEKVESALTPRTRLVALSHVDWTSGEILP
                     LREICALARARDVLTLIDGAQSVGNVPVDAPASGADMYAFTGHKWILGPEGMGALYVR
                     PGLDIFSTNVGFYSLADPLSFDAEGDYELRADARRFEASTASPALCAGFATAAQEVYE
                     RGGPGFEGIRRRAELLIDLLSEIPGVTLRSSWPAASGLVSFEVEGFAAKDVVEHLLEK
                     RFVLRYIPGPRPYVRASTHLFNTEEELGALVRAVSIATRRR"
     gene            2143..>2664
                     /locus_tag="VFT03_10815"
     CDS             2143..>2664
                     /locus_tag="VFT03_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU4848680.1"
                     /translation="MQNGNLRRRGRRRRQARKKSHWLAVARNAFLLLFGASIVGVVVL
                     FASFTQTYAAVAEDMPQLDDYSSTELAQTSVVYDANGNVVDELYGVQNRYVVPLDGID
                     PTLRKAVIAIEDHRFYEHRGLDFEAIGRAAVENIRSLSIQEGGSTITQQLIKNTYIAQ
                     KQRMIPSFERKFAE"
BASE COUNT          484 a          838 c          873 g          469 t
ORIGIN      
        1 ccggcatgcg ggtagccgtg gtcacctcca aacgccgctc ctccgtagag atggccctca
       61 agatcttccc cggcctcggg aaggtggtag accaatgggt cacgatggag gacaccaccg
      121 aacacaagcc ccgccccgaa ccgctcctga aagggttgga gatgctcggc aacgtgccca
      181 gagaacaggc cgcatacgtc ggcgactctc ccttcgacgt caccgcggcg aaggctgccg
      241 gtgtagagag cgttgcggtg agctggggcg ccttttccga ggacgctcta cgcgccgccg
      301 agcccgacca gctcgttccc gacctcgacg cggcggtgga tgtcctcctg tcagcgtaga
      361 aaacaggctt cgatatactg gcggtggtga tctcacgtct tgtcggaaca ctgctcctgg
      421 tcgcggcggc gctcgtcgct ccctacgact gcgggacgac cgcgggttct ggagacgcgg
      481 gtgactcccc cgaaacgaca gcaaccgaag taaaatctaa cgaacctact ctgatgggcg
      541 gtggttccag acccccggat agcaccctct cttacggtgg agagaccgcc cgcggggcgc
      601 tcggaacgta ttgttggaaa agaggttgtg cagacagcgt tggcatcgtg gcctacaaag
      661 acgagctaag ggtgcctgcg ggagcgcccg tgacgttcgc gtacggggga agaaagctcg
      721 actcgatagc agttgtggcc taccggatcg gtcctggaaa ccaaccggaa cagatgggca
      781 gaaagatcct gctcataccc gcggggaagg gtaccgagct cccggcgcga cgtagtggca
      841 accgggcgca gattagtgcg gagttacccg ctggcaggta cgtagtcgat gtcttcgcca
      901 cgatgcctgg gggcgacgct tcctacggct tccttatcgc cgtcgagtag ctatgctcac
      961 cgctcggacc aacgctccta gctcctcctc cgtgttgaac aggtgcgtgc tcgcccggac
     1021 gtagggtcgg ggcccgggga tgtagcgcaa tacgaacctc ttctccagca gatgctccac
     1081 tacatccttc gccgcaaatc cctcgacctc gaaactcacg agccctgaag ctgcgggcca
     1141 ggacgatcta agggtgacgc ccgggatctc cgagaggaga tctatgagca gttcggcgcg
     1201 gcgacgaatc ccctcgaagc ctggtcctcc tcgctcgtag acctcctgcg cagccgtggc
     1261 gaaaccggcg cagagggctg ggctcgccgt cgaggcttcg aagcgtcgcg cgtcagctct
     1321 cagctcgtag tcgccctccg cgtcgaagga gagggggtcg gcaagggagt agaaaccgac
     1381 gttcgtgctg aagatgtcga gcccgggcct gacgtagagt gcgcccatgc cttcgggccc
     1441 caggatccac ttgtgcccgg taaaggcgta catgtcggcg ccggacgcgg gtgcgtccac
     1501 ggggacgttg ccgacggact gggcgccgtc tatcagggtc aacacatccc gcgctcgggc
     1561 gagcgcgcat atctcccgca ggggcaagat ctccccgctt gtccagtcca cgtgcgagag
     1621 ggcgacgaga cgcgttctcg gggtaagggc agactccacc ttctctggcg tcacgggtgg
     1681 tgagacgagc tgtaccttca cgcctgagcg tgccctcagg ttgtggaggg ggaccaggca
     1741 gcccgggtgc tctgtggttg ccgagatgac ttcgtcgcct tcgcgccagt cgatcgacgc
     1801 ggtgccgagg ttcatgccgt gggtggtgtt ctgggtcagg gcgacgtcct gtggggcggc
     1861 cccgataagc tgggcggcgc tctcgcgggc gcgttccctt atatcggcct ggcgggcgta
     1921 gaggccggcg ccctcgaggt aggccgcgcc ggtgcagagg gcgtcggcgg cgcgcatggc
     1981 ttcgatcacg ccgtaggaag gcggtccgct gctgcccgag ttcaggtata cgaaatcgtt
     2041 gctggagagg gccgggaaca tctcgcggcc aagactttcg tgcctgacga tgcgtgcctc
     2101 cttccgggga tggtgggatc ctttctgtat aatatccgct tcatgcagaa cggtaacctg
     2161 cgcaggaggg gccgccggcg ccggcaggcg cgcaagaaga gccactggct ggcggtggcg
     2221 cgaaacgcgt ttctgctgct attcggggcg tcgatcgtcg gggtcgtcgt cctcttcgcg
     2281 agcttcaccc agacctacgc ggctgtcgcg gaggacatgc cgcagctcga cgactactct
     2341 tccaccgagc ttgcccagac ctccgtcgtc tacgatgcca acggcaacgt cgtcgatgaa
     2401 ctctacggcg tccagaaccg ctacgtggta cccctcgacg gcatagaccc gacgctgcgc
     2461 aaggctgtca tcgctatcga ggaccaccgc ttctacgagc atagggggct ggacttcgag
     2521 gccataggcc gcgccgccgt ggagaacata cgcagcctct ccatccagga gggcggctcc
     2581 accataaccc agcagctcat aaagaacacc tacatagccc agaagcagcg catgataccg
     2641 agcttcgaac gcaagttcgc cgag
//
LOCUS       DASTUV010000536         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1053783, whole genome shotgun sequence.
ACCESSION   DASTUV010000536 DASTUV010000000
VERSION     DASTUV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053783"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..2684
                     /locus_tag="VFT03_10820"
     CDS             249..2684
                     /locus_tag="VFT03_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4848681.1"
                     /translation="MSDPIPKDGGTEARDSDESLPERLPPRDLPEELIRRRLARLDTR
                     RALADLCKNVPGLEEVLLPRGIQADALTRKSGAGASLVRGVARRITQDAAAWSAFQTA
                     VQEQIPPETFAALEDFSPEKLEDLSGSHTTEGLLLAALSAEDEPNGEVLASLIGAWRD
                     EHQKAEKRASEDARVRELETELERLKQENEQLSFASRAAKERAAALAEEVEVLTGERE
                     GTSELVRKAEERAASALDVRGQLDDRIAELEKRGAQLERALEGERTAYSRAAERVEEL
                     HEELGRVMAERDRIQDALQNARFTDRGFGELLVRAVKNEVGALPTSIDSTAQTARLLE
                     FMGKVLAAHNDLREPRTAGSTPGPQPSGEDAEDSESSLASAPTSRIEVYARPRPTLSF
                     RALGGAGEVGGSSHLLDFGATRVLVDAGIKPDGRGPLAPNFGKLDRLDAAVITHAHLD
                     HCGALPLLVRDRPELPIYCTPPSAKLIVSALNDHAAMGGGLAGGAPIHEVKKRLMPVP
                     FGKPITVGDARVTLTESGHILGAASVLINSGPATVFHTGDICMEDHFSIPSARLPEVR
                     DIDLLIMEATLADQRPQPFSDSIKKMVDVINETTLEREGSVLIPTYALGQAQEIILGL
                     KHYGREYGLDKDVFIYVDGSVVTTSERLYAEQLGYMKPYLQHTDPRELFFSENIRAVA
                     NDDKARERILTSPCAIIASPVTMQGGASAFYRRRLEKNPKNAVILPSNAASSYGTHHA
                     AGEEGQWQVERVSFAAHCTQEELLGITETLNPRQIILIHGSKRRISDLAFRLAPNHKI
                     HTPAVGETVRTVL"
     gene            2684..3016
                     /locus_tag="VFT03_10825"
     CDS             2684..3016
                     /locus_tag="VFT03_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848682.1"
                     /translation="MELPEVTLLRDARPSRPENAVGISDFWYEPEVWSLPLSPASRVL
                     YAGLCSYLGHGEINRRDLRNTLKDCSSEEVAAALQELVRHSLLVPEPEENLASYAVLS
                     VTQFDAYK"
     gene            complement(3201..>3475)
                     /locus_tag="VFT03_10830"
     CDS             complement(3201..>3475)
                     /locus_tag="VFT03_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848683.1"
                     /translation="HLEGGAVHGKGGRSEQGGHKQCGANSEHHTRGFSVSVHLLRPFF
                     IGFTFFFVASLLILDQAIILHRSLDSPFSMSGGLYGAFAWTVFQIT"
BASE COUNT          699 a         1145 c         1097 g          534 t
ORIGIN      
        1 cccgaggtcg taaccggtct cgtagtagac ggtgcgcagc ccgccgtccc tgagctcgac
       61 gacgaggatg gggtatctgc cgggggagta tgcccggata agcacgtttc cctccgccga
      121 tgacatgccc tgagtttata cgagacccgt ccgcgataga agcccggtaa ggggcgcaca
      181 aagcaccgtg gtagaatcac cgggtatgaa gacgccgtac gacgctcgta ttcgcggagg
      241 gagacctcat gtcagatccc atacccaaag acggaggaac tgaggccaga gactccgacg
      301 agtccctacc cgagagactg ccgccaagag atctgccgga ggagttgatc aggaggcgtc
      361 tggcgcgtct ggacacacgc cgcgcgctcg cggatctctg caagaacgtg cctggactcg
      421 aagaggtcct cctcccccgc ggcatccagg ccgacgccct tacccgcaag tcgggcgctg
      481 gcgcctcgct cgtgcgcggc gtagcgcgcc ggatcacgca ggacgccgcc gcctggagcg
      541 ctttccagac cgccgtacag gagcagatcc cacccgagac cttcgccgcc ctcgaggact
      601 tctcgccgga gaagctggag gacctctccg gctcccacac caccgagggc ctgctcctcg
      661 cggccctgag cgccgaagat gagccaaacg gagaggtcct cgcctccctc ataggagcct
      721 ggcgggacga gcaccagaag gccgagaagc gggcttccga ggacgctcgc gtcagggagc
      781 tcgagaccga gctggagcgc ctcaagcagg agaacgagca gctctcgttc gcctcgcgtg
      841 ccgccaaaga gcgggccgcg gcgctggccg aggaggtcga ggttctcacc ggcgagcgcg
      901 agggcacctc cgagctggta aggaaggcgg aggagcgagc cgcctcggca ctcgacgtgc
      961 ggggccagct cgacgatcgc atcgccgagc tggagaagcg cggcgcccag ctggagcggg
     1021 cgctcgaggg tgagcgtacg gcctattcca gggcagccga gcgagtcgag gagctgcacg
     1081 aggagttggg tcgggtgatg gcggagcggg accgcatcca ggacgccctg cagaacgccc
     1141 ggttcaccga caggggcttc ggggagcttc tggtgagggc cgtcaagaac gaggtaggcg
     1201 cgcttccaac ctctatcgac agcacggccc agaccgcccg cttgctcgag ttcatgggca
     1261 aggtgctggc cgcccacaac gacctgcgag aacccaggac ggccggctcg acacccggtc
     1321 cgcagccttc cggagaggac gccgaagatt cggagtcctc tttagctagc gcccctacat
     1381 ccaggatcga ggtctacgcc aggccgcgtc ctacgctctc tttccgcgcc cttggcgggg
     1441 ccggggaggt gggcggcagc agccacctgc tcgacttcgg cgcgacgagg gtgctggtcg
     1501 acgccggcat caagcccgac ggcaggggac ccctggcgcc gaacttcgga aagctggaca
     1561 ggctcgacgc ggctgtgata acgcacgccc accttgacca ctgtggggcg ttgcctctgc
     1621 tcgtgcgcga caggcccgag ttgccgatct actgcacccc gccgtcggcc aagctcatcg
     1681 tcagcgcgct caacgaccac gcggcgatgg gcggagggct ggctggcggg gcgccgatcc
     1741 acgaggtcaa gaagaggcta atgcccgtcc cgttcggcaa gcccataacg gtaggcgacg
     1801 cgagggtcac gctcaccgag agcggtcaca tcctcggagc cgcgagcgtg ctcatcaact
     1861 cgggtccggc gaccgtcttc cacaccggcg acatctgcat ggaggaccac ttctctattc
     1921 cctcggcccg gctgccggag gtgcgggaca tcgacctcct catcatggag gccaccctgg
     1981 ccgaccagag accccagcca ttctccgact ccataaagaa gatggtggac gtcatcaacg
     2041 agaccaccct ggagcgcgag gggagcgtcc tgatcccgac gtacgccctc ggacaggcgc
     2101 aggagatcat cctcggcctc aagcactacg gacgggaata cggcctggac aaggacgtct
     2161 ttatctacgt tgacggctcg gtcgtaacga cctcggagag gctctacgcc gagcaactcg
     2221 ggtacatgaa gccctacctg caacacaccg acccgcgcga gctcttcttc tcggagaaca
     2281 taagggcggt cgccaacgac gacaaggcgc gcgagcgcat cctcaccagc ccctgcgcga
     2341 tcatcgcctc gccggtcacg atgcagggcg gggcgagcgc cttctaccgc aggaggctgg
     2401 aaaagaaccc gaagaacgcc gtgatcctgc cttccaacgc cgcctcttcc tacgggacgc
     2461 accacgccgc cggcgaggag gggcagtggc aggtcgagcg ggtcagcttc gcggcgcact
     2521 gcacgcagga ggagcttctc ggcataaccg agacgctcaa cccccgccag atcatcctga
     2581 tccacggctc caaacggcgc ataagcgacc tggccttccg gctcgcccct aaccacaaga
     2641 tacacacccc ggccgtcggc gagacggtac gtaccgtcct ttagtggagc tcccggaggt
     2701 cacgctcctc cgggacgcgc gcccttcgcg ccccgaaaac gccgttggga taagcgactt
     2761 ctggtacgag ccggaggtgt ggtctctccc cctctccccc gcctcgcgtg tcctctacgc
     2821 gggcctctgc tcctacctcg ggcacggcga gatcaaccgc cgcgacctcc gaaacaccct
     2881 gaaagattgc tcgagcgaag aggttgcggc ggccctgcag gagctggtgc gccacagcct
     2941 cctcgtccct gaaccggaag agaacctcgc gagctacgcg gtactctccg tgacgcagtt
     3001 cgacgcctac aagtagtttg ttcctctaaa tcaacagact accgctaagc agcttgcgca
     3061 aataggcggg tagattcacc tggcagcaga aaggctgttg catcgctcag acgtctgcca
     3121 ggcaagaaga gttaccaagc tattggcacg gtctctaaac cgttgcgtag cggctttttc
     3181 actaacgttc tcgaaggatt tcaggtgatc tgaaaaaccg tccaggcgaa ggctccgtat
     3241 aaccctccgc tcatagagaa gggcgaatca agtgagcgat ggaggatgat agcctgatca
     3301 aggattaaga gcgaagcgac gaagaaaaac gtgaagccaa taaagaaagg tctaagtagg
     3361 tgaacagaga cagaaaaacc acgcgtgtgg tgctcgctat tggcgccgca ctgcttatgg
     3421 ccgccttgtt cgctgcggcc gcctttgccg tggacagcac ctccttcaag atggt
//
LOCUS       DASTUV010000537         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_768816, whole genome shotgun sequence.
ACCESSION   DASTUV010000537 DASTUV010000000
VERSION     DASTUV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768816"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1718
                     /locus_tag="VFT03_10835"
     CDS             51..1718
                     /locus_tag="VFT03_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU4848684.1"
                     /translation="MSGRSRAGMAGVVGAIVAFGVAELVHGLYPPVPSVFVALAQQVV
                     ERTPGELVTRGIELLGQADIPVLVTSMIVGALVVAGALAHLGVRRPGLALVGVGALAA
                     VGIAASLAQPFVAPVPTVITILAALTLGVAVTETLLRASGLRAATARAQEAAPPDSKE
                     VQPPIARSREAYSSGGTMVGRRGFLMLGGLAAVLGLAAAGVGRALADRGPQASSAPKP
                     LKLPEKSPSETPEKGAGAEEAVKHETLPPPPKDASIDAPGMVPLITPASSFYLIDTAL
                     VSPRIDVNGWKLSVKGAVDNPIELSYKDLLGMSTREADITLSCVSNVVGGGLVSNGRW
                     TGVLLSDVLAEAGVSRDKITRSSRQLVGRGVDGFTTGFRTDIALDGREALVAFGLNGS
                     ELPIKHGYPVRLVVPGLYGYVSATKWLSEIELTSWNFDAYWIQRTWSKEGPIKTQSRI
                     DTVQDGDQLSAGKIPVGGIAWAPHRGIERVELSTDGGQTWNQTRLAKQLAIDTWRQWI
                     YDWDARPGEYTLMVRATDGEGVTQTATQAPPHPSGATGYDTVGVTVA"
     gene            2022..>2372
                     /locus_tag="VFT03_10840"
     CDS             2022..>2372
                     /locus_tag="VFT03_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEU4848685.1"
                     /translation="MKVEKLHGFDVSPAEARRLQGELASGVVAGPPLDLERVRYVAGA
                     DVSTQEGVAYATVAVLDFPGLSVVEVRGFEAALEFPYVPGLLSFREMPSVLGALEKVE
                     TDVDAVILDAQGLAH"
BASE COUNT          390 a          789 c          776 g          417 t
ORIGIN      
        1 tattgttgca agggcgagag cgcaccgggc gagccacagg agagtaggtc atgagcggca
       61 ggtctagagc gggaatggcg ggcgtcgtag gggcgatcgt ggcgttcggc gtcgcggagc
      121 tcgtccacgg cctgtatcct ccggttccct ctgtattcgt ggcgctcgcc cagcaggtgg
      181 tggaacggac gccgggcgag ctcgtgacga ggggcataga gctcctcggt caggccgaca
      241 tacctgttct ggtcacgtcg atgatagtcg gcgctctggt cgtcgccggc gctctggcgc
      301 acctcggcgt ccggcgccct ggcctcgccc ttgtcggggt gggcgcgctg gcggcggtag
      361 gcatcgccgc ctcgctcgcc cagccgttcg tcgctcccgt ccccacggtc atcactatcc
      421 tggccgctct taccctcggc gtcgccgtca ccgagaccct tcttcgcgcc tccggcctcc
      481 gggcggcaac ggcgagggct caggaggccg cgcctccaga ctctaaagag gtgcaacccc
      541 cgatcgcgcg ttcgcgggag gcctactcct cgggcgggac gatggtagga cgccgtggtt
      601 tcctgatgct cggcggcctc gcggcggtac tcggactcgc ggccgcgggc gtgggacgcg
      661 cgctggccga ccggggaccc caggcgtcct ccgccccgaa accgctgaaa ctgcccgaaa
      721 agtctccctc cgagaccccg gaaaagggcg ccggtgcgga ggaggcggtg aagcacgaga
      781 cgctcccgcc tcctcccaaa gacgcctcga tcgacgcgcc ggggatggtg ccccttatca
      841 caccggccag cagcttctac ctcatagaca cagctcttgt ttccccccgg atagacgtga
      901 atggctggaa gctctcggta aagggagctg tggacaaccc gatcgagctc tcctacaaag
      961 acctcctggg catgtccacc cgcgaggccg acatcacgct ctcgtgcgtc tcgaacgtgg
     1021 tcggcggggg gctcgtctcg aacggacgct ggaccggcgt cctgctctcc gacgtgctcg
     1081 ccgaggcggg ggtgagccgc gataagatca cacgctctag cagacagctc gtgggccgcg
     1141 gcgtcgacgg ctttacgacc ggcttcagga cagatatagc cctggacggc cgcgaggcgc
     1201 tcgtcgcctt cggcctgaac ggcagcgagt tgccgatcaa acacggctat ccggtacgcc
     1261 tcgtcgtacc cggcctctac ggctacgtct cggctaccaa gtggctcagc gagatagagc
     1321 tcaccagctg gaacttcgac gcatactgga tccagcgcac ctggtccaag gaaggcccta
     1381 taaagaccca gagccgtata gacaccgtgc aggacggcga tcagctctcc gcaggcaaga
     1441 tccccgtcgg cggtatcgcc tgggcgcccc accgcggcat cgagcgcgtc gagctctcga
     1501 ccgacggcgg ccagacctgg aaccagaccc gcctcgcgaa acagctcgcc atcgacacct
     1561 ggcggcagtg gatctacgac tgggacgccc ggcccggcga atacaccctc atggtccgcg
     1621 ctacagacgg cgaaggcgtg acccagaccg cgacgcaagc cccgccacac ccctccggcg
     1681 ctaccggcta cgatactgtc ggcgtcaccg tcgcctgacc agccccgttg gatgtcttac
     1741 cctgatcttt agcttcaacg tgaggcttta tgcgcgccct ggcacgtcgt gtggcccaaa
     1801 acacggcttt gtaaccaaag tgtttccctt acgttacaaa accgtaacac tatggcaagc
     1861 tcatcgtaac cgggcaactg tagtattcgg attaaaccga gccccggccg atgggaccaa
     1921 tcccccctcc agattccatc ggtcgggctg gtctttctgg ggtggcacgt ctggctcgcc
     1981 ccaactctgc cgggatgttc tgggataatc gcacttgagc catgaaggtt gagaagcttc
     2041 acgggtttga cgtgtcgcct gcggaggcgc ggcgcctgca gggggagctg gcgtccgggg
     2101 tcgtggcggg tccgccgctg gaccttgaac gggtacgcta cgtagccggg gccgacgtct
     2161 cgacgcagga aggcgtggct tacgctacgg tggcggtgct cgacttcccc gggctctccg
     2221 tggtggaggt gcggggtttc gaggcggcgc tcgagttccc gtacgtgccg gggttgttgt
     2281 ctttccgaga gatgccttcg gtcctcgggg ctctggagaa ggtggagact gatgtagacg
     2341 ctgtgatcct ggacgcccag gggctagccc ac
//
LOCUS       DASTUV010000538         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_515052, whole genome shotgun sequence.
ACCESSION   DASTUV010000538 DASTUV010000000
VERSION     DASTUV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515052"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..870)
                     /locus_tag="VFT03_10845"
     CDS             complement(169..870)
                     /locus_tag="VFT03_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848686.1"
                     /translation="MRSRRFLPPRTRDLLREATALALVLGAALLLVACASEGRPPACP
                     PGVGRGMWDFPWSEDGVPADAGVRKLLADRDFGATPQAAQDLEAGVVDERLVAVLRTL
                     TEEHRVCVEAFKEGHFFEPGVPDGPRIPRGYGMAGGLPNTHYFGRAADVYWVDGKPVE
                     GNATDPDVLNIGRALAQMPARVRPDQIIGPPGWTSALSYGRQKGWILERDQLELHEDH
                     LHLGYMDERGTRNGR"
     gene            1117..1602
                     /locus_tag="VFT03_10850"
     CDS             1117..1602
                     /locus_tag="VFT03_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU4848687.1"
                     /translation="MRRIQMTVDGNTHVAEVEPRLLLIHHLRENLGIGGVHSGCDTTN
                     CGACTVLLDGESVKSCTVLAVQADGHEITTVQGITENGLWHPMQQAFHESHGLQCGYC
                     TPGMIMASISLLKEKPNATEEEIREGLEGNLCRCTGYENIVKAVRMAQEHLNQLPTPT
                     S"
     gene            1616..4192
                     /locus_tag="VFT03_10855"
     CDS             1616..4192
                     /locus_tag="VFT03_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU4848688.1"
                     /translation="MTEAPEKYVGGGVRRKEDPALITGQATWVDNIKLPGMLHAAVLR
                     SPFAHARITSIDTSAAEEQPGVVAVFTGEDLADEWFQNPTANPAAVTGYDLSFDQRVQ
                     LGGESVTPMAEQPEEQPVEPGHKTFLAWHVTEDIKVPHHPPVAIEEVNYAGEPVAAVV
                     ATDRYKAQDALEFIEVDYEPLDPVLDLEAAAQEGAPLVHESLGTNHSYTWDLDVGDIE
                     DAFSKADITIKERYIQQRLIPNAIETRGAVANPDPVSGGFTFYSSTQIPHILKAVLTE
                     YCGFYENKLRVVAPDVGGGFGSKLNVYPEEALTLVLSRRLGVPIKWVENRSENYLATI
                     HGRGQVQDIELAATNEGRILGMRVKLLADMGAYLQLNGPGIPLLGAFMYPGVYTFDAY
                     SFECTGIFTNKTPIDAYRGAGRPEAAFAIERIVDALARETGLDPAEVRRRNFYEPFDE
                     PTDTPAGIQYDSMNMQPALDRVLELADYEALREEQRRRREAGDPVQLGIGFSTYTEIC
                     GLAPSQVLAALRAGGAGWEVAKVQMLASGKVEVITGTSPHGQGHVTSWSQIAADTLGV
                     EVDDIEILHGDTAIAPYGRDTYGSRSLPVGGVAVHVAANRVIDKARKIAAHMLEAAEG
                     DIEFQGGRFSVAGSPDRNITIQEVSGQAFLAHDLPEGMDPVLSEEYFFDPPNFTFPFG
                     AHICVVEVDTETGFVKIRDYFAVDDCGTIVNPTIVDGQLHGGIAQGIAQALYEEAVYD
                     EEGNLTTGTMVDYLIPGAPELPRYTLERTVTPSPSNPIGVKGVGEAGTIGSPPAVINA
                     VVDALSLYGITHVDMPASPWRVWQAIQDAQGREAGERDADLSGRPAQATDSADQEGGS
                     AL"
BASE COUNT          771 a         1385 c         1368 g          671 t
ORIGIN      
        1 gacctcctcc agtacctcgt cgtccggtgc gtccgaggct accagcgcct ccacctcggc
       61 ccgcagcttc gagcggtccc ggctgttcat gctctgttct ctcatcggtg gtctcctcgt
      121 cgtcgcgaaa ggattcccgg atatgatagc tcctgacccc ccttcggctc accgcccgtt
      181 gcgggtgccc ctctcgtcca tgtagccgag gtggaggtgg tcctcgtgca gctcgagctg
      241 gtcgcgctcc aggatccatc ccttctgacg cccgtagctc agagcgctcg tccagccagg
      301 cgggcctatg atctggtccg gcctgacccg cgcgggcatc tgcgccagtg cccttccgat
      361 gttcaagaca tccgggtccg tggcgttgcc ctcgacaggc ttcccgtcca cccagtacac
      421 gtcggcggcc ctcccgaagt agtgggtgtt gggcaggccg ccggccatac cgtagcctct
      481 ggggatgcgg ggtccgtcag ggaccccagg ctcgaagaag tggccctcct tgaaggcctc
      541 gacgcagacg cggtgctcct ctgtgagggt ccggaggacg gcgaccagac gctcgtccac
      601 gaccccggcc tcgaggtctt gcgcagcctg cggcgtcgct ccgaagtccc ggtctgcaag
      661 gagcttcctc acccccgcgt cggcaggcac accgtcctcg ctccacggga agtcccacat
      721 accgcgtccg acgcccggag gacaagccgg gggtcggccc tctgaggcgc aggcgacgag
      781 caggagtgct gcacccagga ccagggcgag cgccgtggcc tctctgagca ggtccctcgt
      841 ccgtggcgga agaaagcgtc tcgatctcat ccgggatgat tctacgatgc tcgcggacgg
      901 gccccgcaga cgcacgcgat aagatcgcct gtgctacact gcgcacgtcg gagagaaagg
      961 ggaaaggccg gcaaaaccgc cagaaacacc ggggaaccgc tgctcttgta agcgcgttga
     1021 cggcgacccc ggagcggacc gtagaccctc tgttctgggt gcacggtaag ccgggcgacc
     1081 ccggttctcg gggtcttctc agggaaagga gggcatgtgc gaaggatcca gatgacggtg
     1141 gacgggaata cgcacgtcgc ggaggtggag ccgcgtctct tgctgatcca ccacctcaga
     1201 gagaacctcg gtatcggggg cgtgcactcc gggtgcgaca ccaccaactg cggagcgtgc
     1261 acggtattgt tggacggcga gtcggtcaag tcgtgtacgg tgctcgcggt gcaggccgac
     1321 gggcacgaga tcacgacggt gcagggcatc acggagaacg ggctctggca cccgatgcag
     1381 caggcctttc acgagtcaca cggcttgcag tgcgggtact gcacgccggg catgatcatg
     1441 gcctctataa gcctcctcaa ggagaagccg aacgcgacgg aggaggagat ccgggagggt
     1501 ctcgagggca acctctgccg ctgcaccggc tacgagaaca tcgtgaaggc cgtgcggatg
     1561 gcccaggagc acttgaacca gctgcccacg ccgacttcct gaaggaggta aataagtgac
     1621 cgaagcaccg gaaaaatacg tcggcggcgg tgtacggcgc aaggaggacc ctgccctcat
     1681 aaccggacag gccacctggg tagacaacat caagctgccc ggtatgttgc atgcggccgt
     1741 cctccgcagc cctttcgccc acgccaggat cacatccatc gacacctcgg cagccgagga
     1801 gcaacccgga gtggtcgccg tcttcaccgg tgaggatctg gccgacgagt ggttccagaa
     1861 ccctaccgcc aacccggcgg ccgtaaccgg ctatgacctg agtttcgacc agcgggtgca
     1921 gctgggtggg gaaagcgtca ccccgatggc cgagcaaccc gaagagcagc ccgtcgagcc
     1981 ggggcacaag accttcctcg cctggcacgt tacggaggac ataaaggtcc cgcaccaccc
     2041 gcccgtagcc atcgaggagg tcaactacgc gggagagccc gtggcggcgg tggtggcgac
     2101 cgaccgctac aaggcgcagg acgccctgga gtttatcgag gtcgactacg agccgctgga
     2161 cccggtgctc gatctggagg ccgccgctca ggaaggtgca ccgctcgtcc acgagagcct
     2221 cgggaccaac catagctaca cctgggatct ggatgtcggc gacatcgagg acgcttttag
     2281 taaggccgac atcaccatca aggaacgcta catccagcag cgcttgatac ccaacgcgat
     2341 agagacgcgc ggcgccgtgg caaatcctga cccggtaagt ggaggcttca ccttctactc
     2401 ctcgacccag atccctcaca tcctcaaggc ggtcctcacc gagtactgcg gcttctacga
     2461 gaacaagctg cgggtcgtgg ccccggacgt gggtgggggc ttcggctcca agctcaacgt
     2521 ctacccggaa gaggccctga cgctcgttct ctcgcgcagg ctcggggttc cgatcaagtg
     2581 ggtcgagaac cgctcggaga actacctggc caccatccac gggcgcggcc aagtccagga
     2641 catcgagctg gcggccacga acgaaggcag gatcttgggg atgcgcgtga agctgctcgc
     2701 cgacatgggc gcttacttgc agctcaacgg tcccggcatc cccctgctcg gcgcgttcat
     2761 gtaccccggc gtctacacct tcgacgccta ctcattcgaa tgcaccggta tcttcaccaa
     2821 caagacaccg atcgatgcct accggggcgc gggcaggcct gaggcagctt tcgccataga
     2881 gcgcatcgtg gacgccctcg cccgcgagac cgggctcgac ccggcggagg tacgtcgccg
     2941 caacttctac gaacctttcg acgagccgac cgacaccccg gccggcatcc agtacgactc
     3001 gatgaacatg cagccggcgc tggaccgggt cttggagctc gccgactacg aggccttgcg
     3061 cgaggaacaa cgtcggcgcc gcgaggcggg cgatccggtc cagctcggca taggcttttc
     3121 gacgtacacc gagatctgtg gcctggctcc ctcccaggtg ctcgctgcgc tgcgcgccgg
     3181 cggcgccggc tgggaggtgg cgaaggtcca gatgctcgcc tcgggcaagg tcgaggtcat
     3241 cacgggcact tcgccccacg gccaggggca cgttacgtcc tggtcgcaga ttgctgccga
     3301 cacactcggc gtcgaagtgg acgacatcga gatcctgcac ggcgatacgg ccatcgcccc
     3361 ctacggtcgc gacacctacg gatcgaggag cctccccgta ggcggtgtgg ctgtgcacgt
     3421 ggccgccaac agggtcatcg acaaggccag gaagatagcg gcgcacatgc tggaagccgc
     3481 ggagggagac atagagttcc agggcggtcg cttctcggtc gccgggtctc ccgaccgcaa
     3541 catcacgatc caggaggtct cggggcaagc tttcctggcg cacgacctcc cggaggggat
     3601 ggatccggta ctctcggagg agtacttctt cgacccgccg aacttcacgt tcccgttcgg
     3661 ggcgcacatc tgcgtggtgg aggtggacac cgaaacaggc ttcgtcaaga tccgggacta
     3721 cttcgcggtg gacgactgcg gcacgatcgt caacccgacc attgtggacg gccagctgca
     3781 cggcggcatc gctcagggta tagcgcaggc gctctacgag gaggccgtct acgacgagga
     3841 gggcaacctg acgacgggca cgatggtgga ttacctgatc cccggcgccc cggagctgcc
     3901 gcgctacacc ctggaacgga ccgtcacgcc gtcgccttcg aacccgattg gcgtgaaagg
     3961 cgtcggcgaa gccgggacta tcggctctcc gcccgcggtc ataaacgcgg tagtggatgc
     4021 gctctcgctc tacggcatta cgcacgtaga tatgccggcc tccccctgga gggtgtggca
     4081 ggcgatacag gacgcccagg ggcgcgaggc gggggagcgc gacgccgacc tctcgggccg
     4141 cccggcgcag gcaacggaca gcgccgatca ggagggaggt agcgccttat gattc
//
LOCUS       DASTUV010000539         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_519196, whole genome shotgun sequence.
ACCESSION   DASTUV010000539 DASTUV010000000
VERSION     DASTUV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519196"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2629
                     /gene="dnaE"
                     /locus_tag="VFT03_10860"
     CDS             <1..2629
                     /gene="dnaE"
                     /locus_tag="VFT03_10860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4848689.1"
                     /translation="GLPGPGYRTTDQLYLKPRARMEALFGDRPGALRNAAAVAERCAG
                     AVDLGGEIHVPSARLRGGETARGKLARLALLGARERYGHVEGRVRARLQRELRCIEEL
                     GFAPYFLLAREAAEIARERGIPVTGRGSAANSLVSYCLGLTRPEPFSNRLLFERFMHE
                     FRSDAPDIDLDLCSLRRDEVRDELARRYEEYGVAVAATAQTMSLRGAVRVAARALGHA
                     PSEIDALSRHVPTRFRDRERTYNPLSGWEEALAEPAMRGHPLQDRERYGLLLEVSGRL
                     AGRHLQAGTHNGGLVFGNEHHHLSELAPLEPTAMPSLLRVQYDKDDLEYAGMPKLDLL
                     GLRMHTALHEAGVLASRRAGKRVDPYDPPPDDRETYALIRTGRNAGMFQLESPGQMHL
                     SRRLGPRRFSDLVAQISLFRPGPVRGDLVTPYVLRKQGREQYSVPLPELGPVLRSTYG
                     VLVFQEQVLEIACVVAGFSLAEGDQIRRAMTRDRGPGAMKALREEFVSRAVSRGVPRA
                     TAREVFSWTEGFAAYGFSAAHAASFAELAYASAYMRAHFPAEYLAAVLNAQPMGFYSP
                     RAVLNEVRRIGLSVLPADIHLSGEGFTEEEDGTALRVGLKYARGLSGKALSSILAERS
                     KAPFSSTLDLYRRTAVGRGALENLVKGGFLDALSPTGDAGGRAALLAEVSVLPKKPPP
                     SWRNQDELPLPHPARRSRQKGVVELRGPGFLRLSAARQETMEWESLSLNVLRHPLLPY
                     RQALRGLGVTPSPEILELPHGTRARAAGLLESLQRPPTRSGRPVYFLLIEDEAGLFQA
                     TIFEGVYKRDGHVLHQRGAFLLDGRVEQDKRRGFSFLVERVRDLSRALEAAGRVPEPQ
                     ATPSPGAFLRAKRRGRRAG"
BASE COUNT          474 a         1043 c         1016 g          435 t
ORIGIN      
        1 cggtctcccc ggtcccggct accggacgac cgatcagctc tacctcaagc ctcgggcgag
       61 gatggaagcg ctcttcggcg acaggcccgg cgctctccgc aacgccgccg cggtggccga
      121 gcgctgcgcc ggcgccgtcg acctgggagg ggagatccac gtgccatcgg cccggctgcg
      181 cgggggcgag acagctcgtg ggaagctcgc ccggctcgcg ctcctggggg caagagagag
      241 gtacggtcac gtcgaaggcc gggtgcgcgc caggctccaa cgcgagctca ggtgcataga
      301 ggagctcggc ttcgccccgt acttcctcct cgcccgcgag gcagcggaga tagcacggga
      361 gaggggcata cccgtgacgg gccgggggag cgcggccaac tcgctcgtct cctactgcct
      421 ggggctgacc cggcctgagc ccttctccaa ccgcctcctc ttcgagcgct tcatgcacga
      481 gttccgctcc gacgctccgg acatagacct ggacctgtgc tctttgcggc gcgatgaggt
      541 ccgcgacgag ctcgcgagac gctacgagga gtatggcgta gcggtggctg cgacggcgca
      601 gacgatgtcg ctgcgaggcg cggtgcgagt agccgcccgg gcgctcggcc acgccccgag
      661 cgagatagac gcgctctcga ggcacgtgcc gacgcgtttc agggaccgcg aacggaccta
      721 caacccgctc agcggctggg aggaggcgct ggccgagccc gctatgcgag gccaccccct
      781 gcaagatagg gagagatacg ggctcctgct ggaggtctcg gggaggctcg ccggacgcca
      841 cctccaggcc gggacccaca acggcggcct ggtcttcggc aacgagcacc accacctctc
      901 cgagctcgcg cccctggagc ccacggccat gcccagcctc ctcagggtcc agtacgacaa
      961 ggacgacctg gagtacgccg ggatgccgaa gctagatctc ctgggcctcc ggatgcacac
     1021 ggcgctccac gaggctggcg tcctcgcctc caggagggcg gggaagaggg ttgaccccta
     1081 cgacccgccg cctgatgaca gggagacata cgccctgatc cggaccggca ggaacgccgg
     1141 gatgttccag ctcgagagcc ccggccagat gcacctttcc aggcgcctgg gtccccgccg
     1201 cttctccgac cttgtggccc agataagcct cttcaggcca ggcccggtga ggggcgacct
     1261 ggtgacgccc tacgtcttga ggaagcaggg cagggagcag tattccgtgc cgctgccgga
     1321 gctcgggccc gtgctgcgct ccacctacgg cgtcctcgtc ttccaggagc aggtcctgga
     1381 gatagcgtgc gtggtcgccg gcttctcgct cgcggaggga gaccagatcc ggcgcgccat
     1441 gacccgcgac cgcggccccg gcgccatgaa ggcgctgcgc gaggagttcg tctcccgagc
     1501 ggtctcacgc ggggtgccca gggctacggc gcgtgaggtc ttctcctgga ccgagggctt
     1561 cgccgcctac ggtttctccg ccgcccacgc tgcctccttc gccgagctcg cctacgcgtc
     1621 ggcctacatg agagcccact tccccgcgga gtacctcgcc gccgtcctga acgcccagcc
     1681 catgggcttc tattccccgc gcgcggtcct caacgaggtg cgcaggatcg ggctctcggt
     1741 attgcccgcg gacatccacc tctccggcga gggcttcacc gaagaggaag acggcaccgc
     1801 ccttcgcgtg ggcctcaagt acgccagagg gctctccgga aaggccctct cttccatcct
     1861 cgctgagagg agcaaggctc ccttctcctc cacgcttgac ctgtaccgca ggaccgcggt
     1921 cggtcgcggc gctttagaga acctggtcaa aggcggcttc ctggacgctc tctccccgac
     1981 gggggacgcc ggcgggcgcg ccgcgctgct ggccgaggtg agcgtcctgc cgaagaagcc
     2041 acctccctcg tggcgaaacc aggacgagct gcccctcccg catccggcgc gccggagcag
     2101 gcagaagggc gtcgtcgagc tgagaggacc cggcttcctc cgcctctcgg ccgcccggca
     2161 ggagaccatg gagtgggagt cgctctccct caacgtcttg aggcaccctc tattacccta
     2221 ccgtcaggcg ctccgcgggc tcggcgtgac cccgagcccg gagatcctcg agttgcccca
     2281 cgggacgcgg gccagggcgg ccgggctcct ggaatcgttg cagaggcccc cgaccaggag
     2341 cggtcgcccc gtctacttcc tgctcatcga agacgaggcc ggattgttcc aggccaccat
     2401 cttcgagggc gtctacaaga gggacggaca cgtgctgcac cagcgcggcg ccttcctcct
     2461 agacggcagg gtggagcagg acaagaggcg cgggttctcc ttcctggtgg agcgggtgcg
     2521 ggacctctcg agggcgctag aggccgcagg aagggtgccg gaaccacaag caactccctc
     2581 cccgggcgcc ttcttgcgag cgaagcggcg ggggaggaga gcgggatagt aactgcggcg
     2641 gcacccggag ggatacgtcg aactgtgttt ccgcgccccg cggaccgagg acgacccggc
     2701 tctgggatta cgcgacgctc ctggagctcg cggttctggg caatcgcgag caggaggcct
     2761 tcgacgcgct cgccgacgcc ctggcgaaca tccgcgagtc ctgggacccg aaacaaccct
     2821 gcgtaacctg cgcctcatcc gtaaccgcag ggaacagcgt ggcgaagccg taccctgggc
     2881 catagagatc gaacgagagc tggaagcccg ggcggctacc tcagggcccg atccaggcgc
     2941 acggaggctg tgatcaggga gaggtggg
//
LOCUS       DASTUV010000540         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1556134, whole genome shotgun sequence.
ACCESSION   DASTUV010000540 DASTUV010000000
VERSION     DASTUV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556134"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFT03_10865"
     CDS             <1..697
                     /locus_tag="VFT03_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848690.1"
                     /translation="IGFGVIINAMFKMYPKRTILGLTLMGSQAFLYNAIFFTYALVLT
                     KFYNIDASHVPYYIFPFAIGNVLGPWLLGRLFDTVGRVPMIAGCYLVSGLLLAGTGYL
                     FYLGTLNAVTQTALWCVIFFFASAAASAGYLTVSEVFPMEIRAMAIALFYAIATALGG
                     ISGPVIFGNLIGTGNVTNLFIAYLLSAGLMIFAALVEVFLGVRAEGQSLENVARPLTA
                     IEEGTGTSAAAKA"
     gene            768..1370
                     /locus_tag="VFT03_10870"
     CDS             768..1370
                     /locus_tag="VFT03_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4848691.1"
                     /translation="MRPLADEVQALVARVGTHPVWGYAHCLRVHALAEELARAERLSY
                     DAEILRIAALLHDVGLYKAYAFREAPDHARRSADVAERVLKDGDFPPHATRAVIEAIL
                     SHPPGVPPGGSVESALLKDAVALDYLGAIGLSRILAMVGIEDDVADIPAALWQAESLR
                     RKIPGFLVFESSKGIARERILEMDDFLRDLKGATKDLKLI"
     gene            complement(1648..1866)
                     /locus_tag="VFT03_10875"
     CDS             complement(1648..1866)
                     /locus_tag="VFT03_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848692.1"
                     /translation="MSPTLAFGLLLALAGVAGLSFGLYALLRGGRGQEGGIGPISERG
                     VHVVAGVRMLVVGALSLATGVYLIWSSL"
     gene            complement(1876..3219)
                     /locus_tag="VFT03_10880"
     CDS             complement(1876..3219)
                     /locus_tag="VFT03_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4848693.1"
                     /translation="MEYEEFLEQGEILAGEGLIEEALARFEQALAAAPENPEVIEAVG
                     RALLNLDRLEEAEASFLDALELDPEWVGPRMGLAMVALRRDEPFKIVHHLERAIETDP
                     EYPDAYVELGRYYGAMAEPALAKATFERWTARHPEDADMLINAGLTAFDAADYAQALD
                     FFEKAVEAALVEEQKSGARTFRANTLDMLGRYDEAVEAYEEVIAETPEWWEAHANLGI
                     CHARNGQSARAEAAFRRGLEDCPGSPEIRDELAAHLLSERRDLPEALRLSEEAVALGR
                     DEIRHLHTLGEARLALGDEAGAAEAYSAMLALDPENPEAHLELGLLYEARGEAREAEE
                     HFVESLKSDPANPRALYSYASLYYAADDLETAEELLVRAVASDAGYSPALSALASIRA
                     RRGDYRDALDYIEKAVEAGESDVEHFKSALEFAPLRSDPRFRSLLSRMVRMGDEG"
     gene            3463..3936
                     /locus_tag="VFT03_10885"
     CDS             3463..3936
                     /locus_tag="VFT03_10885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU4848694.1"
                     /translation="MKHPPVIARISIRYNDLDPYGHVNNAAYLEFFDEIRLAYWRALA
                     DLTGFDELEAGDVPGARYVIAETTVRYKVPIFLDDALHGAAIVPTVGNRSYAMDFELR
                     TGETFEEGLVVAEGFAAHAFFDPSTDEVKPRPDWFLPAVAELEGRPEEAFIPVTE"
BASE COUNT          691 a         1367 c         1342 g          697 t
ORIGIN      
        1 catagggttc ggcgtgatca taaatgccat gttcaagatg tacccgaagc gcaccatcct
       61 cgggctgacc ctcatggggt cgcaggcctt cctctacaac gccatcttct ttacctacgc
      121 gctggttctg acgaagttct acaacataga cgccagccac gtcccgtact acatcttccc
      181 gttcgctatc ggcaacgttc tcggcccgtg gctcctcggg cgcctcttcg acacggtggg
      241 ccgggtgccg atgatcgcgg gttgctacct agtctcgggc ctgttgctgg ccgggaccgg
      301 ctacctattc tacctgggaa ccctgaacgc ggtcacgcag acggccctgt ggtgcgtcat
      361 tttcttcttc gcctccgcgg ccgccagcgc tggctacctg accgtgagcg aggtcttccc
      421 gatggagata agggccatgg ccatagcgct cttctacgcc atagccacgg ctctgggtgg
      481 gatctcgggt cctgtaatct tcggcaacct cataggcacg ggcaacgtca cgaacttgtt
      541 catcgcctac ctcctgagcg cggggttgat gatcttcgca gccctcgtag aggtgttcct
      601 cggcgtgagg gccgaggggc agtccctcga gaacgtcgcg aggccactca cggccatcga
      661 ggagggcaca ggcacgagcg ctgcagccaa ggcgtgatat aagagcggtc gcaaaaaccg
      721 cgcctcctgc gcggtcattc gtgtccgtct gctacactag cgaacccatg aggccgctgg
      781 cggacgaggt ccaggcgctg gtcgcccggg tggggaccca ccccgtctgg ggctacgccc
      841 actgcctgcg ggtccacgcg ctggccgagg agctagcaag ggcggagcgg ctctcctacg
      901 acgccgagat cctgcgcatc gccgccctcc tgcacgatgt ggggctctac aaggcctacg
      961 cgttccgcga ggctcccgac cacgccaggc gctcggcgga cgtcgcagag cgggtcctga
     1021 aggacgggga cttccctccg catgctacga gagcagtcat cgaggccatc ctgagccacc
     1081 ccccgggagt cccgcccggc ggctcggtgg agtccgcgct cctcaaggac gccgtcgccc
     1141 tcgactacct cggggctata ggcctctcgc gcatcctcgc catggtgggc attgaggacg
     1201 acgtagccga cataccggcc gcgctgtggc aggcggagag cctgcgacgg aagataccgg
     1261 gtttcctcgt cttcgagagc agcaagggta tagccaggga gcgcatcctg gagatggacg
     1321 acttcctgcg cgacctgaaa ggcgccacga aggacctgaa gcttatttga gcatgtcagc
     1381 acgcctgctt cgcaggcttg tcagctatga tggcgcactt cgtcgctcca cagcgtcaga
     1441 caaggctact gtccgaaagc cgtctcgtaa cacgaaaccg ggcacgaggc gaacggcaga
     1501 agaagctgaa atgctgagaa gcgagggcct ttactaaagg cccgaagcgt gctgagaagg
     1561 cgtctggcga agccaggcgc cgtgctgaca agcttcccga agggaggcgt gctgacttca
     1621 ccctggtgct cccccggaac ggcccgtcta gagactagac cagatgaggt agacgcctgt
     1681 agcgaggctc agagcgccga cgaccagcat ccttacaccg gcgacgacat ggacgccgcg
     1741 ctcggagatg gggccgatgc cgccctcctg ccctcggcca ccgcgcagca gggcgtagag
     1801 gccgaaggag aggcccgcca cgcctgcgag cgcgagaagg aggccgaagg ccagggtggg
     1861 ggacacgagc tcctgctagc cctcgtcccc catacgtacc atgcgggaga ggagggagcg
     1921 gaagcggggg tcggagcgta agggtgcgaa ctcgagggcg ctcttgaagt gctccacgtc
     1981 gctctcgccg gcctctaccg ctttttcgat gtagtcgagc gcgtcgcggt agtcgccgcg
     2041 gcgggcccgg atgctggcga gcgccgagag ggcaggagag taacccgcgt cagaggcgac
     2101 ggcccgcacg agcagctcct cggcggtctc caggtcgtcg gcggcgtagt agaggctggc
     2161 gtaggagtac agggcgcggg ggttcgcggg gtcgctcttg agggattcca cgaagtgctc
     2221 ctcggcctcc ctcgcctcgc cgcgcgcctc gtacaggagc ccgagctcca ggtgcgcctc
     2281 ggggttctca gggtcgaggg cgagcatcgc gctgtaggcc tcggcggccc cggcctcgtc
     2341 gcctaaggcc agacgggcct ctccaagggt gtggaggtgc cggatctcat cgcgcccaag
     2401 cgccacagcc tcctccgaga gcctgagcgc ctcggggagg tccctccgct cggagaggag
     2461 gtgggcggcg agctcgtccc ttatctccgg cgagccggga cagtcctcga ggccccgccg
     2521 gaacgcggcc tccgctctcg ccgactggcc gttgcgggcg tggcagatcc cgaggttcgc
     2581 gtgcgcctcc caccactccg gcgtctcggc tatgacctcc tcgtaggcct cgacagcctc
     2641 gtcgtagcgg ccgagcatgt ccagtgtgtt ggccctgaag gtgcgcgctc cgctcttctg
     2701 ctcctccacc aaggctgcct caacggcctt ctcgaagaag tcgagcgcct gggcgtagtc
     2761 ggcggcgtcg aaggcggtga ggccggcgtt aatgagcatg tctgcgtcct ccgggtggcg
     2821 cgccgtccag cgctcgaagg tggctttagc aagggcgggc tccgccatgg caccgtagta
     2881 gcggccgagc tccacgtagg cgtcgggata ctcggggtcg gtctctatcg cccgctcgag
     2941 gtggtgcacg atcttgaacg gctcgtcgcg cctgagcgcg accatggcca ggcccatccg
     3001 gggcccgacc cactctggat ccagctccag ggcgtcaagg aagctcgcct cggcctcctc
     3061 gagcctgtcc aggttcagga gggcgcggcc gacggcctct atcacctcgg ggttctcggg
     3121 ggccgccgcc agcgcctgct cgaagcgggc gagggcctcc tcgatcaaac cctccccggc
     3181 gaggatctcg ccctgctcta gaaactcttc gtactccatc ccaccagatt aaaccagatg
     3241 acaggacctg ggagcgcgat gcttctacgc cacgttgaga tcctctctag ggatttcaaa
     3301 gataaattcc cggaggcgcg gatgcagtat gctgtatgaa gggagaccca cagacgctcc
     3361 aggggccgcc ctggggggcg ctttccggcc tagagcggca aagtgacgca accagtcttg
     3421 cagtacaatc ttgcgggcat ggatccggca ggggaggagc ctttgaagca cccgcccgtg
     3481 atcgcgagga tctcgatccg gtacaacgac ctcgacccct acggccacgt caacaacgcc
     3541 gcctacctgg agttcttcga cgagatacgg ctggcttact ggcgggctct ggcggacctc
     3601 acaggcttcg atgagctcga agcgggcgac gtccccggcg cccggtacgt gatcgccgag
     3661 acgaccgtcc gctataaggt tccgatcttc ctcgacgacg ccctccacgg cgccgcgatc
     3721 gtccccaccg tcggcaaccg ctcctacgct atggacttcg agctccgtac gggcgagacc
     3781 ttcgaagaag gcctcgtcgt cgccgagggc ttcgccgccc acgccttctt cgaccccagt
     3841 acagacgagg taaagccccg ccccgactgg ttcctccccg ctgtcgccga gctcgaaggc
     3901 cgccccgaag aagccttcat ccctgtgacg gagtagcttt ggagcgccgc ggtctcggaa
     3961 ggagcggagt ggaggtcccc gtcgtgggcc tggggacctg gcaggtcctc gacgttcgcg
     4021 gcgccgagga agaggaagcg cgtcacgagg tcgtccgggt cgcgctcgac gaggacgcca
     4081 acctcttcga ctcttcc
//
LOCUS       DASTUV010000541         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1126567, whole genome shotgun sequence.
ACCESSION   DASTUV010000541 DASTUV010000000
VERSION     DASTUV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126567"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..993
                     /locus_tag="VFT03_10890"
     CDS             427..993
                     /locus_tag="VFT03_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848695.1"
                     /translation="MSTSGLIRGSGLAALLGGALWVVVFAIYALRASGPGAEPPYRSF
                     EGLGVPGVLSLLLIALGFMGLDWPTWRGEGYGRLGRGLGRVGFGLALLGVFVIVGSGA
                     SGTVGIIGVVVLMVASLLVGAAALLTSVLPRWGAIVLIVGSLTFFLFDTETARAWCAV
                     PYGVAWIVVGYLQWAGRGVELQGPTRAR"
     gene            complement(1196..2740)
                     /locus_tag="VFT03_10895"
     CDS             complement(1196..2740)
                     /locus_tag="VFT03_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU4848696.1"
                     /translation="MKTRERLDPVTLSVLTSALAGIAEEMGAVLIRGAYSSNIKERRD
                     CSTALFDGRGRMVAQAEHIPVHLGAMPEAVAAIMRHDPRPGDVFAINDPYSGGTHLPD
                     ITLVSPVAPKGEIIGYAVTRAHHSDVGGMRPGSMPSDSREIYQEGIIIPPLRLVRGGE
                     YVDDILDFILANVRTPEIRRGDLRAQIAANRIAEERVGELIERRGEEIVLAAFDEVIA
                     YTERRTREAIRELPDGVYRAEDFLEGDGTVEDEDIPIRAKVRIKDDSITIDFAGTADS
                     VRGNVNCPLPVTRSACYFALRVLLPKDMPANAGTYAPLEIRAPEGSLVNAKSPSAVVA
                     GNVETSNRIADAVLAALSEAASLPAQGQGTMNNTIIGGPGWTYYETIGGGQGASEKGP
                     GPSGVHVGMSNTLNTPIEAFELEYPMRVERYELLYGSGGEGKHRGGDGIVRSVRVLEP
                     ASLSLLTDRRRHPPRGANGGGPGKVGGNFLNEEKLPPKVSRELKAGDVVTVRTPGGGG
                     YGRAEE"
     gene            complement(2737..4716)
                     /locus_tag="VFT03_10900"
     CDS             complement(2737..4716)
                     /locus_tag="VFT03_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU4848697.1"
                     /translation="MAPTRLGVDVGGTFTDLVALSEGGVTTSKVPSTPRDQSIGVMNA
                     IGASGVEAGALDALAHGMTVATNALLERRGARMALVTTEGFRDVLEIARQTRPSLYDL
                     TQDRPPTLVLRDLRFTLKERMGSEGELSPLDEAGLKDVLSAIEEAEVEAVAVCLLFAF
                     MHPEHERRVGEALREALPSVHVSLSSEVLPEFREYERFSTTAADAYLAPKLAAYLKNL
                     AGKVEDAGMPTPLIMQSAGGVVGIEDAVNNASAIVLSGPAGGVVGAAYVGTLSGYRDL
                     LTFDMGGTSTDVAPIIGGEAQTTTETVIAGVPIKVPMVDVHTVSAGGGSIAWADAGGA
                     LRVGPHSAGAEPGPAGYNKGGQEPAVTDANLFLGYLADRAELGGEVVLRRELSEEALQ
                     KLGNKVGLNALETALGIVRVADAEMVRALRVISVERGLDPREFALLAFGGAGGMHACR
                     LAEELGMRTVLVPRAGGVLSALGLAISDLRRDYVSPYLADLADVNVRELKELFEGMEG
                     TAAKDLEGPRHTRRADLRYRGQSFELMVEAEKPLEIDKLEESFHAVHERRYGYRMEDE
                     PVELVNLRLIATVPVDKPELRESPAEGEAESGRREANFDGEWQEVPILDRERMGKGSE
                     VVGPAIVEFREATCVVRPGWQGLVDGVGTLVLEKE"
     gene            complement(4716..>4962)
                     /locus_tag="VFT03_10905"
     CDS             complement(4716..>4962)
                     /locus_tag="VFT03_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848698.1"
                     /translation="LFPTRVRYGGYSIGYNFSVAIFGGSAPFLATWLISTTGNPLSPA
                     FYVIFAAVATLLTVLTVRETARTDLLKTQPHLEEVAR"
BASE COUNT          739 a         1610 c         1648 g          965 t
ORIGIN      
        1 taaaggggag gcggggctct cttgggaact agaaggtagt gcgatgggcc gggagcaatg
       61 ggagcgtaag tgcgataaag cccgtcgctg tagccgtctg gttcggcctt taggagatag
      121 gagaagagac atgctccagc tgcatccagc tgcagcgttt ctctcaccac gcgcaccggc
      181 tcctcgtaca gcctgtgcag gtcggtctcc tcgcgcagcc ctggcggaga acgcagcccc
      241 agggtctttg ctacgccagc ttttctacac tactttagtg aagaagtccc aagaggcgaa
      301 cggggcatcc gaactgcgga gaatttccct tccacgcact tgggtgaata ggatgtctga
      361 agaggcgtag agcttcgttc ggtggcatca tgtccttgtg ttagaggtag cgagaagaga
      421 ggtgctatgt ctacttcggg ccttatccgg gggagcggcc tagcggctct attaggcggg
      481 gcgctgtggg tggtggtgtt cgccatctac gcgttgaggg cctcgggacc gggggcggaa
      541 cctccttaca ggagctttga gggccttggt gttcctggcg tgctttccct gctgctcatc
      601 gctttgggct tcatgggcct tgattggccg acctggcggg gggaaggtta cgggaggctc
      661 gggagggggc tcgggagggt aggcttcggt ctggccctac tcggggtctt cgtgatagtc
      721 ggcagcggcg cctcagggac tgtgggaata atcggggttg tcgtcctgat ggttgcctcg
      781 ctattggtgg gtgcggccgc tctgcttacg agcgtgcttc cccgctgggg ggcaatagtg
      841 ctcatagttg gctcgctgac gttctttttg tttgacaccg agactgcccg ggcgtggtgc
      901 gcggtgccct acggggtggc gtggatagtg gtgggctacc tgcagtgggc tggtcgggga
      961 gtggagctgc agggacctac acgcgctaga tgaacttctg agaattcacc ttctacgagg
     1021 ttgggtgaat agaggggtgg ggctacctcc tgcccctggc cgtaatctgg atggtccagc
     1081 ccttgtgtct cctccttggt tgtttgctcc ggatgccccg ctctctaggc tagcgggtcc
     1141 gccgacccgc aagacgacac gcccgtaaac gcgagaacgc cgtccggttc ggagcttact
     1201 cttcggctcg tccgtacccg ccgccgcccg gcgtgcggac cgtaacgacg tctccggcct
     1261 tgagctcccg gctgaccttc ggcggcaact tctcctcgtt gaggaagttc ccgcccactt
     1321 tccccggtcc cccgccgttc gctccgcgtg gcgggtggcg ccggcggtcg gtgagcaagg
     1381 agaggctggc gggctccagt acccgcacgg agcgaactat gccgtcgccg ccccggtgct
     1441 tgccctctcc gccggagccg tagaggagct cgtagcgttc gacgcgcatc gggtattcga
     1501 gctcgaaggc ctctatcggg gtgttgaggg tgttgctcat cccgacgtgg acgccggagg
     1561 gaccggggcc tttctcactt gctccctggc cgccgccgat ggtctcgtag taggtccagc
     1621 ccgggccgcc gatgatcgtg ttgttcatcg tcccctggcc ctgcgcgggc agcgaggcgg
     1681 cctcggagag tgcggcgaga acggcgtccg cgatgcggtt gctggtctcg acgttcccgg
     1741 ccacgacggc cgaagggctc ttggcgttca cgaggctgcc ttccggggcg cgtatctcca
     1801 gcggcgcgta ggtgcctgcg tttgcgggca tgtccttggg taagagtacg cgcagggcga
     1861 agtagcacgc cgagcgcgtc accggcagcg gacagttgac gttgccccgc acggagtcgg
     1921 cggtgccggc gaagtctatg gttatcgagt cgtcctttat cctcaccttc gcccggatgg
     1981 ggatgtcctc gtcttctacc gtgccgtcgc cctcgaggaa gtcctcggcg cggtagacgc
     2041 cgtccgggag ctcccggatc gcctcgcggg tgcgcctctc ggtgtaggcg atgacctcgt
     2101 cgaaggcggc gagcacgatc tcttcgccgc ggcgttcgat gagctcaccc acccgctctt
     2161 cggcgatgcg gttggcagct atctgggcgc ggaggtcccc gcgccggatc tcgggggtgc
     2221 gcacgttggc gaggatgaag tccagaatgt cgtctacgta ctcgccgccc ctgaccagcc
     2281 tgagcggcgg gatgatgatg ccctcctggt agatctcgcg cgaatcgctg ggcatcgagc
     2341 cggggcgcat cccgcccaca tcggagtggt gcgcccgggt cacggcgtag ccgatgatct
     2401 cgcctttagg ggccactggc gagacgagcg ttatgtccgg caggtgcgtg cccccggagt
     2461 aggggtcgtt tatcgcgaag acgtcaccgg gcctaggatc gtggcgcatg atcgccgcca
     2521 ccgcctcggg catcgccccc aggtgcaccg ggatgtgctc ggcctgggcg accatcctgc
     2581 ccctaccgtc gaagagcgcc gtcgagcagt cgcgccgctc ttttatgttc gaggagtagg
     2641 cgccccggat caggaccgcc cccatctcct cggctattcc cgccaaggcg ctcgtcagca
     2701 ccgagagggt tacgggatct agtcgttctc tggtcttcat tccttctcca atacgagcgt
     2761 gcccacgccg tccaccaggc cctgccagcc gggccttacg acgcaggtcg cctccctgaa
     2821 ctccactatc gccggtccga cgacctccga acctttgccc atccgctcgc ggtccaggat
     2881 cggcacctcc tgccactcgc cgtcgaagtt ggcctcccgg cgtccggact cggcctcgcc
     2941 ctccgctgga gactctctca gttccggttt gtccaccggt acggtggcga tgaggcgcag
     3001 gttcaccagc tctacgggtt cgtcctccat ccggtagccg tatcgccgct cgtgtactgc
     3061 gtggaagctc tcctccagct tgtctatctc caggggcttc tcggcctcga ccatgagttc
     3121 gaaggactgt cccctgtagc gcaggtccgc ccggcgggtg tgtcttggcc cttccaggtc
     3181 tttggcggcc gtgccctcca tgccttcgaa gagctccttg agctccctga cgttcacgtc
     3241 cgcgaggtcc gcgaggtaag ggctcacgta gtcccggcgc aggtcggaga tcgcgagccc
     3301 gagcgcgctc aggacccctc ccgctcgggg gacgagcacg gtcctcatgc cgagttcttc
     3361 ggcgagccgg caagcgtgca tgcccccggc cccaccgaag gccaggaggg cgaactcccg
     3421 ggggtcgagg ccgcgctcga cgctgatcac gcgtagcgcc cgcaccatct ccgcgtcggc
     3481 cacccgcacg atccctaaag cagtctcgag agcgttaagg cccaccttat tgcccagctt
     3541 ctggagggcc tcctcagaaa gttcgcggcg cagcaccacc tcgccgccga gctccgcccg
     3601 atccgcgagg tatccgagaa acagattcgc gtcggtgacg gccggctctt ggccgccctt
     3661 gttgtatccg gcgggaccgg gctcggcgcc tgccgagtgg ggcccgacgc gtagagcccc
     3721 gcccgcgtcc gcccaggcga tggagccccc gccggcgctc accgtgtgca cgtccaccat
     3781 gggaaccttg atcggcacgc ccgctatcac cgtctccgtg gtcgtctgcg cctcgccacc
     3841 gatgatgggc gccacatccg tgctcgtccc gcccatatcg aaagttagaa gatcccggta
     3901 gccgctgagg gttccgacgt aagccgcgcc cacgacccca ccggccgggc ccgaaaggac
     3961 aatggcggag gcgttgttca ctgcgtcttc tatcccaacc accccgcccg cggactgcat
     4021 gatcaggggg gtcggcatgc cggcatcctc caccttgccg gccaaattct ttagataggc
     4081 ggcgagcttg ggggccaggt acgcgtcggc ggcggtcgtg gagaatcgct cgtactccct
     4141 gaactcgggc agcacctcgc tagagagcga gacgtgcacg ctggggagcg cctcccttag
     4201 cgcctcgccg acccttcttt cgtgctccgg atgcatgaac gcgaagagta ggcatacggc
     4261 gacggcttcc acctccgctt cttcgatggc ggagagcacg tctttaagac cggcttcgtc
     4321 gagcggtgag agttcgcctt ccgagcccat cctctccttg agcgtgaagc gcaagtcgcg
     4381 caggaccagg gtggggggcc tatcctgggt gaggtcgtag agggaggggc gggtctggcg
     4441 cgcgatctcc agcacatccc ggaagccctc cgtcgtcacg agcgccatcc gcgcgccgcg
     4501 gcgctcgagc agggcgttgg tcgcgacggt catgccgtgg gccagggcgt ccagagcacc
     4561 ggcctccacc cccgaggcac ctatggcgtt catcacgcct atagactggt ctcgcggcgt
     4621 cgaaggaacc ttcgacgtgg tcactcctcc ctcggagagc gccaccaggt cggtgaacgt
     4681 gccgcccacg tcaaccccga gccgtgttgg tgccatcaac gtgccacctc ttcgagatgt
     4741 ggctgcgtct tcaggagatc ggttcgggct gtctctctga ccgtcaagac cgtgaggagc
     4801 gtcgccacgg cggcgaatat cacgtagaaa gcgggcgaga gggggttacc ggttgtggag
     4861 atcagccagg tcgctaagaa tggcgcgctc cccccgaaga tcgccaccga gaagttgtaa
     4921 ccgatggagt agccgccgta gcgcacgcgc gtggggaata gt
//
LOCUS       DASTUV010000542         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_841034, whole genome shotgun sequence.
ACCESSION   DASTUV010000542 DASTUV010000000
VERSION     DASTUV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841034"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFT03_10910"
     CDS             complement(<1..623)
                     /locus_tag="VFT03_10910"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEU4848699.1"
                     /translation="MDNRYISLEWRPELERPVLIAAFTGWNDAAEAASVALAALRDSW
                     EARNFGVFDAEEFFDYQAARPRIKLVEGVTRTIEWPENQLSATAPSLESAGGRGAVLL
                     SGPEPSFRWRSFCAAVIDLARELNVELVVTMGALLADVPHSRPVSVAANSQDPALVES
                     LGLSASRYEGPTGITGVLHRACAEASLSSVSFWAPVPHYLPAIPSAPA"
     gene            complement(707..1279)
                     /gene="aat"
                     /locus_tag="VFT03_10915"
     CDS             complement(707..1279)
                     /gene="aat"
                     /locus_tag="VFT03_10915"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU4848700.1"
                     /translation="MRLTPEVLEACYRAGAFPMDDGYGRTGFYRSDPRSILELDALHV
                     SKSLARVVRKGTYELRVDSDFEAVIRACADRRDTWIGAEIIRSFVRFHELGKAHSVEA
                     YRGGELAGGLYGVALGGAFMGESMFSRMPDASKVCLVHLVERLKSRGYVLLDCQIQND
                     HLARLGAVEIPEREFLRRLEHALSLDRSFV"
     gene            complement(1366..>2320)
                     /locus_tag="VFT03_10920"
     CDS             complement(1366..>2320)
                     /locus_tag="VFT03_10920"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEU4848701.1"
                     /translation="NRLAAAGLAPSANGAHQTSGLPVGSPAPTFTLDDLDGEKVALDG
                     LRARGKPVMLVFTDPDCETCMELLPDVGRWQQQYGGKLTIAVVGLGSVEENSAEASEH
                     ALENVLLQEDWEVADAYEVDATPSAVVLSPEGIISSPVAEGPEEVAALLAYAAGERAQ
                     LPLFQAAAGHAADDEEEDDEPEGPEVGEPAPEIRLQDLEGRDVSLEDFRGEEVLVLFC
                     SPGCGFCQEMMPDIKQWEAAPPEGAPRLLVVSDDTVEENKAIGLSSPVVLDNTDAVWD
                     AYDVSGTPSAVLVDADGRIASRMAVGSEEVLELIKSGRTAA"
BASE COUNT          392 a          783 c          759 g          386 t
ORIGIN      
        1 gccggggccg agggtatggc cgggaggtaa tgcggcactg gagcccagaa gctgaccgaa
       61 gacaagctgg cctcggcgca cgcgcggtgc aggacgcctg tgatgcccgt cgggccctcg
      121 tagcgcgagg cggagagccc cagagactcg accagggccg gatcctggga gttggcggcc
      181 acagagaccg ggcgcgagtg gggcacgtca gcgagcaggg cccccatggt gacgacgagc
      241 tcaacgttga gctccctggc cagatcaatc accgccgcgc agaacgagcg ccaccggaag
      301 ctcggctccg gcccggagag caatacggct ccgcgcccgc cggcggactc gaggctcggc
      361 gcggtcgcgg agagctggtt ctccggccat tcgatggtac gcgtcacgcc ctcgaccagc
      421 tttatccggg gcctggccgc ctggtagtcg aagaactcct ccgcgtcgaa gaccccgaag
      481 ttccgggcct cccaactgtc cctcagcgcc gccagcgcga cgctagcggc ctccgccgcg
      541 tcgttccaac ccgtgaaagc ggcgatcaat acaggccgtt cgagctccgg ccgccactcc
      601 agacttatgt acctgttgtc cataaggcta gcttatcacg caccggtcta agcatggttt
      661 cacaggcgga agatgagctt cgcgctacct tccacggcct gattgatcag acgaaggatc
      721 tatcgaggct cagggcgtgc tcgaggcgcc tgaggaactc gcgctcgggg atctcgacgg
      781 ccccgagacg ggccagatga tcgttctgga tctggcagtc aaggagcacg tagcccctgc
      841 tcttgagacg ctcgacgagg tggacgaggc aaaccttcga ggcgtcgggc atgcggctga
      901 acatcgactc ccccatgaac gccccgccta aagcaacgcc gtagagacct ccggccagct
      961 cgccgcccct gtacgcctcg acgctgtggg ccttcccgag ctcgtggaag cgaacgaagg
     1021 agcggatgat ctcggcgccg atccaggtgt cacggcggtc ggcgcacgcc cttatgactg
     1081 cctcgaagtc gctgtcgacg cggagctcgt aggtcccttt gcggacgacc cgggcgaggg
     1141 acttcgagac gtgcagggcg tcgagctcga ggatggatct cgggtcggag cggtagaaac
     1201 ctgtgcgccc gtacccgtcg tccatgggga atgcaccggc ccggtaacac gcctcgagaa
     1261 cctctggtgt gagcctcatg gcccaatagt agcggaggtg gagcgcgccg gccgcagcga
     1321 ctgcgcgacc cgaactctca ggctataagg ttctccgatc tcacgtcatg cggcggtccg
     1381 gccggacttg atcagctcga gcacttcctc cgatcccacg gccatcctcg aagcgatgcg
     1441 cccatccgcg tcgacgagca cagccgacgg cgtgcctgag acatcgtagg cgtcccacac
     1501 cgcgtcggtg ttgtccagca ccaccggaga gctgaggccg atggccttgt tctcttcgac
     1561 ggtatcgtcc gagacgacca gcaatctggg agcaccctcg ggtggggcgg cctcccactg
     1621 cttgatgtcg ggcatcattt cctggcagaa gccgcagccc gggctgcaga agagcaccag
     1681 gacctcttca cccctgaaat cctcgaggct gacgtccctg ccctcgaggt cctggagtct
     1741 gatctccggg gctggctcgc cgacctccgg gccctctggc tcatcatctt cttcttcatc
     1801 gtccgcggcg tgaccggcgg cggcttggaa cagcggcagt tgagcgcgct ctcccgccgc
     1861 atacgcgagg agagctgcga cctcctcggg accctcggcc acgggactcg agatgattcc
     1921 ttcgggactg aggaccaccg cgctgggcgt agcgtctacc tcgtaggcgt cggccacctc
     1981 ccagtcttcc tggagcagca cgttctccag cgcgtgctcg gaggcctcgg cgctgttctc
     2041 ctcgacgctg cccaagccta cgaccgcgat cgtgagcttc ccgccgtact gctgctgcca
     2101 gcgtccgacg tcggggagca gttccataca ggtttcgcag tccgggtcgg tgaacacgag
     2161 catgaccggc ttgccgcggg cgcgcaagcc gtcgagggct accttttcgc cgtcgaggtc
     2221 gtccagggtg aaggtagggg ccggagagcc aactggcagc ccggacgtct gatgggctcc
     2281 gttcgccgac ggtgccaggc ccgcggcggc gagcctgtta
//
LOCUS       DASTUV010000543         5805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_297214, whole genome shotgun sequence.
ACCESSION   DASTUV010000543 DASTUV010000000
VERSION     DASTUV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297214"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFT03_10925"
     CDS             <1..811
                     /locus_tag="VFT03_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4848702.1"
                     /translation="AVVAIIALGQTFVIVSGGIDLSVGSVLGLAGIVFGWASVVANLP
                     LMAALALGIGAGALAGLTNGLLITLGNLPPFIATLAMLSAARGLALVISGGVPLNPIP
                     GAILSLGSGDLFGFVPLPVVLMLVMWALTIGILRSTYAGRCMYAIGGNEEASRLSGIN
                     VSRQKLIVYTLSGLFAGIAGILLTARLASADPQAGFTYELDAIAAVVIGGASLSGGVG
                     NASGTLIGALILGVLRNGLNLLNVSAFWQQVIIGAVIALAVMTDTLRRRKS"
     gene            824..1765
                     /locus_tag="VFT03_10930"
     CDS             824..1765
                     /locus_tag="VFT03_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4848703.1"
                     /translation="MRTRATLLAVLMLAILATAFAAGCQRGGGGGGEGEGPRVGLSIS
                     TLNNPFFVSMRSGAQQAAKDEGVQLLIADAQNDSATQQDDVQNFVTQQVDAILVNPVD
                     SEAIVPSIQAANQANIPVIALDRGASGGEIATLIASNNVEGGTMAGEELIRLVGSGPV
                     AQLEGIPGASPTRDRGQGFEEAINAQNAVKLVASQTANFDRAEGLDVTENIMQSNPEI
                     KGIFAQNDEMALGAVRALGGRAGTDVKIVGFDGVEDALNAVQDGKMNATVAQQPDKIG
                     SLGVENAMKVIDGESVPKNIPVEVKLVTKENVSQFLQ"
     gene            1773..2678
                     /gene="rbsK"
                     /locus_tag="VFT03_10935"
     CDS             1773..2678
                     /gene="rbsK"
                     /locus_tag="VFT03_10935"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEU4848704.1"
                     /translation="MSNIFVLGSINQDFVLKVERRPEPGETVTNAELSTHNGGKGANQ
                     AAAAALLGASVAFLGRVGDDEFGEPLVQALEEKGIDTILIKPASGTPTGAAFITVTPD
                     GENAITVAPGANRSLTPGDVEAAADEIGEAGVFVAQMEVPVQAVLRAVEVAAEQGTRA
                     LVNLAPTFEVPRELLEKLDPLVVNEHEAAFLLGREVEGVKGALSAAPELLSSGPRSAV
                     ITIGEDGAVLADGESVEHLPAPEVDVVDTTGAGDAFVGALATQIARGASLQEAVAYAV
                     RAGAAAVTKEGAQGALPTPEVVEAL"
     gene            2675..3061
                     /gene="rbsD"
                     /locus_tag="VFT03_10940"
     CDS             2675..3061
                     /gene="rbsD"
                     /locus_tag="VFT03_10940"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEU4848705.1"
                     /translation="MKKEGIINARLAGALAGLSHTDHVVVCDAGLPIPPGPEVVDLAF
                     RFGVPSFETVLSGVLEELVIEGATAAEEVDRNPRSYEFLTTRLPDLGLVPHEELKRMV
                     PGAKLVIRTGEATPYSNVILRCGVPF"
     gene            complement(3247..4068)
                     /locus_tag="VFT03_10945"
     CDS             complement(3247..4068)
                     /locus_tag="VFT03_10945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848706.1"
                     /translation="MFNRELLPIVRPHPSDPAGLVFAEGTEQNGVILAGKVRSPSGET
                     YEVKNGYLDLLKNRAGADNAANLTSFLPGAGRGYEPLWRVHSLGLLTGEPFSNERELG
                     IISDLAGVERGGRYLDLGCSAGLYTRNLAPGDGGEVIGIDISPSMLKEAARRARGTGT
                     RPSFARADAHNLPFADACFSGAVCGGTLNELGDPARALRETRRVLEPGGRLAIMGILR
                     ARTLRGRRLQRFLSTSGIRFFEPDELRSMLDHTGFEPAVLRTYGAVFFVGATSRG"
     gene            complement(4076..4705)
                     /locus_tag="VFT03_10950"
     CDS             complement(4076..4705)
                     /locus_tag="VFT03_10950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU4848707.1"
                     /translation="MPADEPRGADEHGWEVHESSADALRGAMRLFPTGVTVVTSGREE
                     RAEGMTANAVVSVSLDPPLFLVSVHKDARLNPRIREEGYFAANILAAEQEGLSRLFAS
                     PERSSGLPAIRSLGGGYGSTGAPLATGALAVIECELEAVHPGGDHDLFVGRVVAVRMG
                     DTRREPLIYHEGGYTTPRPVQRPGEAGAFVDSVRGFDARLQRLRERKRR"
     gene            complement(4695..5462)
                     /locus_tag="VFT03_10955"
     CDS             complement(4695..5462)
                     /locus_tag="VFT03_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848708.1"
                     /translation="MAGDPIVLVGGLAAWPGRYRSLAQSLHEISGAEVYTAPITPLDW
                     LLGRLRGYGQLVFEVASVVDRALLESESDKAVLVGHSMGGIACRIYLGGDPPFGGRRY
                     SGYRRVRRLITLGTPHLVAKENLTLISRVNDYFPGTLHEPAGLEYLSVAGAGADGATS
                     ARVRRRYERLVEDGRVAGDGVVPVESALLPGSEALVLDDLYHNGFYGRWYGSDLAAVE
                     RWWPGDLRLDANLVGEHRAQSRLRTPSHTPRRGEDAG"
BASE COUNT          990 a         1878 c         1975 g          962 t
ORIGIN      
        1 tgcggtggtc gccataatcg ccctcggcca gaccttcgtg atcgtctcgg ggggcataga
       61 cctctccgtc gggagcgtcc tgggactagc cggcatagtc ttcggctggg cgagcgttgt
      121 tgccaatctg ccgctaatgg cggcgctggc gctcgggatc ggagcaggag cgctggccgg
      181 gctcacgaac ggattgctga taaccctcgg caacctgccg ccgttcatcg ccactctagc
      241 gatgctcagc gcggcgcggg ggctggcgct cgtgatctct ggtggcgtcc cgctgaaccc
      301 gataccaggc gcgattctct ctttgggcag cggggatctc ttcggcttcg taccgctgcc
      361 ggtggtcctg atgctcgtca tgtgggccct caccatcggg atcctcagga gcacctacgc
      421 gggccgctgc atgtacgcaa tcggcggtaa tgaggaagcc tccaggctct ccgggataaa
      481 cgtctcgcgc cagaagctca tcgtttacac gctctccggg ctcttcgcag gaatagccgg
      541 catcctgctc accgcccggc tggcttcggc tgatccgcag gccggcttca cctacgagct
      601 ggacgcgatc gctgcggtcg tgatcggcgg cgccagcctc tcgggcgggg tgggaaacgc
      661 ttcggggaca ctgatcgggg cgctcatcct gggcgtactt cgcaacggct tgaacctgct
      721 gaacgtctcg gcgttctggc agcaggtgat cataggcgcc gttatcgccc tcgcggtgat
      781 gacggacacc ctgagacgca ggaagagcta gaaggagggc acgatgagaa cgagggcgac
      841 gttgctggcg gtactcatgc tggcgattct ggcgacggcc tttgcggccg gctgccagcg
      901 cggtggcgga ggaggagggg agggagaagg tccgagggtc ggactctcta tctctacgct
      961 gaacaacccg ttcttcgtga gcatgcgcag cggcgctcag caggccgcga aggacgaggg
     1021 ggtccagctc ctcatcgccg acgcgcagaa cgactcggcc acgcagcaag atgacgtgca
     1081 gaacttcgtt acccagcagg tcgatgccat cctggtcaac cccgtcgact cggaggcgat
     1141 agttccgtcg atccaggccg ccaaccaggc gaacatcccc gtgatcgcgc ttgaccgtgg
     1201 cgcctcgggc ggcgagatcg ctacccttat agcctcgaat aacgtggaag gcggcacgat
     1261 ggcgggggag gagctaatcc gactcgtcgg cagcggcccc gtagcccagc tcgagggtat
     1321 ccccggcgcc agcccaacgc gcgacagggg gcagggcttc gaggaggcga ttaacgcgca
     1381 gaatgcggtc aagctcgtgg cgagccagac ggcgaacttc gaccgggcgg aggggctgga
     1441 tgtgacggag aacatcatgc agtccaaccc ggagatcaag ggcatcttcg cccagaacga
     1501 cgagatggcc ctgggcgccg tacgggccct cggaggcagg gccggcacag acgttaagat
     1561 cgtcggcttc gacggcgtcg aagacgcgct gaatgccgtc caggatggca agatgaacgc
     1621 cacggtcgcc cagcagccgg acaagatcgg ctcccttggc gtagagaacg ccatgaaggt
     1681 catcgatggt gagtccgtgc cgaagaacat ccccgtggag gtgaagctgg tgacgaagga
     1741 gaacgtctcg cagttcctcc agtaggggcc ccatgtccaa catcttcgta ctgggatcta
     1801 ttaaccagga cttcgtcctg aaggtcgagc gccgccccga gccgggcgag acggtcacga
     1861 acgccgagct ctccacgcac aacggcggca agggcgcgaa ccaggctgcc gccgctgctc
     1921 tcctcggcgc ctccgtggcg ttcctgggcc gtgtgggcga cgacgagttc ggcgaaccac
     1981 tggtgcaggc gctcgaggaa aagggcatag acacgatcct aatcaagccg gcttcgggca
     2041 cgccaaccgg cgccgccttc ataaccgtca cgccagacgg ggagaacgcg atcacagtcg
     2101 ctccaggcgc gaaccgcagc ctcaccccag gggacgtgga ggccgctgcg gacgaaattg
     2161 gggaagccgg ggtcttcgtg gcgcagatgg aggtccccgt gcaggcggtg ttgcgggcgg
     2221 tcgaggtcgc ggccgagcag ggcaccaggg cgctggttaa cctggcgcct acgttcgagg
     2281 tcccgagaga gctcctcgag aagctcgacc cgctcgtggt gaacgagcac gaggccgcct
     2341 tcctgctcgg gagggaggtc gagggagtga aaggtgcgct ctccgccgct cctgagctac
     2401 tctcttcagg tccgcggtcc gctgtgatca cgatcggaga agacggagcc gttctcgcgg
     2461 acggcgagtc cgtagaacac cttcccgccc ccgaggtcga cgtagtagac acgaccggcg
     2521 cgggggacgc cttcgtcgga gccctggcca cgcaaattgc ccgcggagct tctttgcaag
     2581 aagctgtagc ctacgccgtg cgagcgggcg cggcggcggt taccaaggag ggcgcccagg
     2641 gcgcgctccc gacccctgag gtggtggagg cgctatgaaa aaggaaggca tcataaacgc
     2701 gaggctcgct ggagccctgg ccgggctgag ccacaccgac cacgtcgtcg tctgcgacgc
     2761 cggcctgcct ataccaccgg gcccagaggt cgtggatcta gcgttccggt tcggtgtccc
     2821 ctcgttcgaa accgtgctct ccggcgtgct cgaagagctg gtcatcgaag gcgccaccgc
     2881 cgccgaagag gtggacagga accctcgctc ctacgaattc ctgacgactc gcctgccgga
     2941 ccttggcttg gttccgcacg aagagctcaa gcgcatggtc ccgggggcga agctcgtgat
     3001 ccgtaccggc gaggctacgc cctactcgaa cgtcatcctc cgctgcggag tgcctttcta
     3061 gcgcgcaagg ggtaggggcg cgcccctacc ccttgcgctt cagcacgcct gcttcgcagg
     3121 cttgtcagca cgctcgggcc tttttaaagg ccctcgctat tcagcatttc agctttttgt
     3181 gaggcgcggg ctggtggcac tcgccctgga tgaacggcgg gctgacccca atctacccgc
     3241 cgaagatcaa cctctgctag ttgcgcctac gaagaagact gcgccgtaag ttcgtaacac
     3301 ggcaggctcg aagcctgtgt ggtcgagcat gcttcttaac tcgtcgggct cgaagaaccg
     3361 gatgccgctt gtggagagga agcgctggag acgccgcccg cgcagggtcc gggcacgcaa
     3421 gatacccatg atggcgagac gtcctcccgg ctcgagtacg cgccgcgtct ctctcagagc
     3481 gcgcgccggg tccccgagct cgttcagggt acctccacag acggccccgg agaagcaggc
     3541 gtcggcgaac ggaaggttgt gggcgtcagc ccgcgcgaaa gagggtctgg tgccggtgcc
     3601 acgggcgcgg cgggcggcct ctttgagcat ggaaggggag atgtctatgc cgattacttc
     3661 gccgccgtcg ccgggtgcga ggttgcgcgt gtagagaccg gcggagcatc cgaggtcgag
     3721 gtaccggccg ccgcgctcca cgccggcgag gtcggagatg atccccagct cgcgctcgtt
     3781 ggagaaaggc tcccctgtga gcaggccgag ggaatggacg cgccagagcg gctcgtagcc
     3841 ccgcccggcg ccggggagga agctggtcag gttggcggcg ttgtccgcgc cggcgcggtt
     3901 cttcagcagg tcgaggtaac cgttcttcac ctcgtaggtc tcgccggagg gagaacggac
     3961 ctttccggcc aggatcacac cgttctgctc cgtgccctcc gcgaacacga gccctgcggg
     4021 atctgaaggg tgcggccgga cgatagggag tagctcccgg ttgaacatcc cggctctagc
     4081 ggcgctttct ttctctgagg cgctgcaggc gggcgtcgaa gccgcggacg gagtctacga
     4141 acgcgccggc ctccccggga cgttgcacgg gtctgggggt ggtgtatccg ccctcgtggt
     4201 agatcagagg ttccctgcga gtgtcgccca tcctgacggc tacgacccgc ccgacgaaga
     4261 ggtcgtggtc gccgccgggg tggaccgcct cgagctcgca ctctatgacc gcgagcgccc
     4321 ccgtcgcgag cggggcgccg gtagagccgt agccgccgcc tagagaccgg atcgccggca
     4381 gcccgctcga gcgctcgggg gaggcgaaga gcctggataa cccctcctgt tcggcggcca
     4441 ggatattggc ggcgaagtag ccctcctcgc ggatacgggg gtttagacgg gcgtccttgt
     4501 ggacgctcac caggaagagt ggggggtcca gggagacgga gacaaccgcg ttcgccgtca
     4561 tgccctcggc tctttcctcg cggcccgagg tgaccactgt gacgccggtg gggaagaggc
     4621 gcatcgcacc cctcaaggcg tccgcgctgg actcgtgaac ctcccaaccg tgctcgtcag
     4681 cgccacgcgg ctcgtcagcc ggcatcttcg cctctcctcg gggtgtgtga tggcgtccga
     4741 agcctgctct gtgcacggtg ctcgcccaca aggttcgcgt caaggcgcag gtctccaggc
     4801 caccaccgct cgaccgcagc cagatccgag ccgtaccacc gcccgtagaa cccgttgtga
     4861 tagaggtcat cgaggacgag agcctccgag ccgggtagca gcgccgattc caccggcacg
     4921 accccgtcgc ctgccaccct gccatcctcc accagacgct cgtatctcct gcgaacgcgg
     4981 gccgaggtcg ctccgtcagc gccagcgcca gccacggaca ggtattcgag gccggccggt
     5041 tcgtgcaggg tgcccgggaa atagtcattg accctgctaa tgagggtgag gttttcttta
     5101 gcgacgaggt gaggggtgcc cagtgtgatc aggcgcctca cccgccggta gcccgagtag
     5161 cggcggccgc cgaagggagg atctccgccg aggtatatcc ggcaggcgat gccgcccata
     5221 gagtgtccca cgagcacggc cttgtccgac tcgctctcga gcagcgcgcg gtctacgacg
     5281 ctggcgacct cgaagaccag ttgcccgtag cctctgaggc gtcccagcag ccagtcaagg
     5341 ggtgtgatcg gggccgtgta cacctctgcg ccggagatct cgtgtaggct ctgcgcgaga
     5401 ctccggtacc tccccggcca cgccgccaat ccgccgacca ggactatggg atcgcctgcc
     5461 atgcaagaga gtataaacgt ctcagccggc agggtatcta ctggagcacc tcgagagccg
     5521 tccgcaccga gtcgtcgagg acggcccggt ccactccggc tctcgcgagg accctcacgc
     5581 cgtgcatggt gcttactagg aaacgggcga gagccttcga gtctttgttg gcgggacctc
     5641 accggcggcg caccagcgcc cgctcgaacg tgtcctcggt gcggcgtacg tagcgagaga
     5701 tcctccccgc tacctcctcg tcgtgcggcg cgagctccac ggccgtgttc gccaggaggc
     5761 acccgcggcg cggctcgtag gagacgagcc cctctatggt cgttt
//
LOCUS       DASTUV010000544         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_912439, whole genome shotgun sequence.
ACCESSION   DASTUV010000544 DASTUV010000000
VERSION     DASTUV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912439"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..262)
                     /locus_tag="VFT03_10960"
     CDS             complement(59..262)
                     /locus_tag="VFT03_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU4848709.1"
                     /translation="MAQGTVKWFNDEKGYGFISPDDGGEDLFVHYSGIEGDGFRTLDE
                     GARVTFEKTQGKKGMQAVNVLPA"
     gene            complement(373..1014)
                     /locus_tag="VFT03_10965"
     CDS             complement(373..1014)
                     /locus_tag="VFT03_10965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4848710.1"
                     /translation="MARFVVLDVDGTLMDTNYLHTEAWARAFEEVGHRIPRARLHKQI
                     GKGSSLLIREFVEDEETEERIDELHSEIYAELQEYGHPLPGAKELISSLRERGYEVWF
                     VTSAKDEELEHHMQELEAESGISGVVNSSAVENPKPAPDIFELALQKAGASPDETVAV
                     GDAVWDVEAADAAGIRTVAVLTGGAFSRQELEEAGAVAVYEDCAELLDSSFPE"
     gene            1052..2056
                     /locus_tag="VFT03_10970"
     CDS             1052..2056
                     /locus_tag="VFT03_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848711.1"
                     /translation="MEDPRPSQLRDRPDTGEAVYTRSSDGSVSDEYAALREGAALVDL
                     SYRKILRLSGKKTVEMLNAVLTNQVPEEDRLGVYALLLNPKGRIQADLRVLKSGEDIL
                     IAVEPEGAAAAKELLGRYAPFYRVSLEDLSDASWGVLGLYGPRAGDLLEAPYLAEHES
                     AEVSLNGADLLAAGVALPVGGYDLLGPSDALEAARNHLRERGAVAAGLDVYETARIEA
                     GVPRFGSDITPENFPAEAGILERAVSFEKGCYPGQETVARMHYRGHPNKELYRFVVEG
                     TPPAPGAEVAQSGRQVGWITSVAPLPVEGQILVLGYLHRNADPRGELESGETQLRNLA
                     "
     gene            2371..>2772
                     /locus_tag="VFT03_10975"
     CDS             2371..>2772
                     /locus_tag="VFT03_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4848712.1"
                     /translation="MLKIENLRVEIDGNEIVKGLDLEVGKGEIHAIMGPNGSGKSTFA
                     NVLLGHPRYEVTDGTVTFEGEDVLELEPDERAKLGMFLAFQYPSEVPGVSVANFLRTA
                     VNSVREEELSPMEMYRLLQEKMAIMHMDPKFA"
BASE COUNT          499 a          896 c          852 g          525 t
ORIGIN      
        1 ccgggaaata agaaggccgg gcgagatgcc cggcccactc accagctctg ctcctgtttc
       61 aggcgggaag gacgtttacg gcctgcatac ccttcttgcc ctgggtcttc tcgaacgtta
      121 cgcgggcgcc ttcatcgagc gtcctgaacc cgtcgccctc tatgccggag tagtggacga
      181 agaggtcttc accaccgtcg tccggggaga taaagccgta gcccttctcg tcgttgaacc
      241 acttcaccgt tccctgggcc atgcgcttgc gcccgccttt ctttcctggg ccgaccgatc
      301 aaaatcggcc ctctttcaga cagcgatgcg cactctagta caaaaccggg cgggctgcac
      361 gcgccgatta atctactccg ggaaactcga gtcgagcagc tcggcacagt cctcgtagac
      421 agcgactgcg ccggcttcct cgagctcttg ccggctgaac gccccgcccg tcaggacggc
      481 gacggtgcgg atgccggccg cgtccgccgc ctcgacgtcc cagacggcgt ctcccacggc
      541 gactgtttcg tccggcgagg cgccggcctt ctgcaaggca agctcgaaga tgtcgggggc
      601 gggcttcggg ttctcgacgg cgctggagtt gacgacgccg gagatcccgc tttccgcctc
      661 gagctcttgc atgtggtgct cgagctcctc gtccttggcg ctcgtgacga accatacttc
      721 gtagccccgc tcgcgcaggg aggagatcaa ctccttcgcc cccggtagcg ggtggccgta
      781 ctcctgcagc tccgcgtaga tctcgctgtg cagctcgtcg attctctcct ccgtctcctc
      841 gtcctcgacg aactctcgga tcaaaaggct cgagcccttc cctatctgct tgtggagccg
      901 ggctctcggt atccggtgcc cgacctcctc gaacgcccgc gcccacgcct cggtgtgcag
      961 gtagttggtg tccatcagcg tgccgtctac gtcgagcacc acgaacctcg ccatctgcga
     1021 cctcctcgac atcatggctt aaaataccgg catggaagat ccgagacctt ctcagcttcg
     1081 ggatcgcccg gacaccggcg aggcagtgta tacccgctcc tcggacggtt cagtctccga
     1141 tgagtacgcc gccctcaggg agggcgctgc ccttgtggac ctctcataca ggaagatcct
     1201 gcggctctcc gggaagaaga ccgtggagat gctgaacgct gtcctcacaa accaggtgcc
     1261 cgaagaagac cgcctcggtg tctacgctct gctcctcaac ccgaagggcc gcatccaggc
     1321 cgacctgcgc gtcctgaaga gcggtgaaga catcctgatc gcggtcgagc cagagggagc
     1381 cgccgcagcg aaggagctgc ttggccgcta cgctcctttc taccgcgtga gcctcgaaga
     1441 cctctccgat gcgtcctggg gcgtcctcgg cctctacgga ccccgggcgg gagacctcct
     1501 cgaagctccc tacctcgccg agcacgagag cgccgaggtg tctctcaacg gcgcggacct
     1561 gctcgccgcg ggcgttgctt tgcccgtcgg cggctacgat ctcctcggtc cgagcgacgc
     1621 tctggaggcg gctcgaaacc atctccggga acggggggcg gtcgcggcgg gcctcgatgt
     1681 atacgaaacg gcgcgcatcg aagccggcgt cccccggttc ggatcggaca tcacgcccga
     1741 gaacttccca gcggaggccg gcatcctgga gcgggccgta agcttcgaaa agggttgcta
     1801 ccccggacag gagaccgtcg cccggatgca ctaccgcggc cacccgaaca aggagctcta
     1861 tcgcttcgta gtcgagggca cccctcccgc gccgggcgct gaagttgcgc agagcggcag
     1921 gcaggtcggt tggatcacca gcgtcgcgcc tctgcccgta gaggggcaga tcctggtcct
     1981 cggttacctg catcgcaacg ctgacccgcg aggcgaactc gagtccggtg agacccagct
     2041 tcggaacctc gcctaacctc gaagcgcacg ggaatcccta tctagaggcc tactccgaac
     2101 gccccaacta ccctcttatc gggaatgatt cccgataaag ccggtcttgc ttgttacaag
     2161 ctgcttctgc gtggtcgctg atgacaacgg ctcgcagttg gatgcattgt gggacaagtg
     2221 gtggtcgaaa accgtttggg aatgccgagt tctgggcaca tcaagaaggg ttattgatcc
     2281 aggtcacgcc gcattgttag gggcaggatg ctgtgattaa atagccctgt aatgtagcaa
     2341 attatgtaac gcgcgagcgt taggggttcg atgctaaaga tagagaatct gcgggttgag
     2401 atcgacggca acgagatcgt gaagggtttg gatctcgagg tcggcaaggg tgagattcac
     2461 gcgatcatgg gcccgaacgg ctcggggaag agcacgttcg cgaacgtgct tctggggcat
     2521 ccccgctacg aggtgaccga cggtacggtc accttcgagg gtgaggacgt actcgagctc
     2581 gagcccgacg agcgagccaa gctggggatg ttcctcgcgt tccagtaccc gagcgaggtt
     2641 cccggcgtga gcgtagcgaa cttcctgcgc acggcggtca actcggtacg tgaggaggag
     2701 ctctctccca tggagatgta caggctcctg caggagaaga tggccatcat gcacatggac
     2761 cccaagttcg cc
//
LOCUS       DASTUV010000545         5649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_518894, whole genome shotgun sequence.
ACCESSION   DASTUV010000545 DASTUV010000000
VERSION     DASTUV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518894"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFT03_10980"
     CDS             <1..417
                     /locus_tag="VFT03_10980"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU4848713.1"
                     /translation="GDSLAGKKVLIPRALVAREILPEKLREAGAEVAVPPAYESVPSS
                     EGADGLARRLEAGEVDCVTFTASSTVENFVGAFGEEEARRLLLGTRVACIGPITADTA
                     RRRGIRVDVEAREYTIPGLVEAVVDLFAADPAKRGE"
     gene            420..1400
                     /gene="hemB"
                     /locus_tag="VFT03_10985"
     CDS             420..1400
                     /gene="hemB"
                     /locus_tag="VFT03_10985"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU4848714.1"
                     /translation="MPFPIQRLRRTRRTGALRGLVRETRLSPSDLVYPIFVTVGEDVK
                     NPVASMPGVFQLSINHAVAEAKRASDLGVPAVLLFGIPNQKDEAASGAYDPEGIVQLA
                     TRAIKDAAPELIVITDVCLCEYTSHGHCGVVEKGTGEVMNDVSLELLARTASSQAEAG
                     ADIVAPSDMMDGRVAAIRSELDNEGFQDTPILAYAAKYASAFYGPFREAAESAPAFGD
                     RRSYQMDPANAREALLEVELDVEEGADIVMVKPALPYLDVVRRVRETTKLPVAAYSVS
                     GEYAMIKAASQNGWFDERTAVLEALTGVKRAGADIIVTYHAPDVARWLSA"
     gene            1477..2802
                     /gene="hemL"
                     /locus_tag="VFT03_10990"
     CDS             1477..2802
                     /gene="hemL"
                     /locus_tag="VFT03_10990"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU4848715.1"
                     /translation="MNERGGDVDARTMLAMDRSARLMERALRRMPGGVNSPVRAFRSV
                     GGDPLFAQRGEGSRIIDVDGNSYVDYVMSYGPLVLGHAHPEVIEAIERTARKGTTFGA
                     PTELEVELAELVCEAVPSVEVVRMTNSGTEATMSAIRLARGFTDREKILKFDGNYHGH
                     GDALLVSAGSGVATLGLPDSPGVTKGAARDTAVLPYNELGAVRELFEREGEAFAAVIL
                     EPVAGNMGCVPPAEGYLEGLREITSEYATLLIFDEVMTGFRLARGGAQERFGVTPDLT
                     TLGKIIGGGLPVGAYGGRREIMDLVAPTGPVYQAGTLSGNPLAMAAGLATLRATGEPG
                     FYERLEELGRRWRQGMNEAASAGGVPFAINQVGSMASIFFTGGPVTDFAAAASSDAEA
                     FKDFFWHMISRGVYLAPSQYEAGFISTAHSERDLEMTCEAAGEWFARGV"
     gene            2804..3232
                     /locus_tag="VFT03_10995"
     CDS             2804..3232
                     /locus_tag="VFT03_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848716.1"
                     /translation="METARRTPEASLKEIFLMAASRLPGEDDRAGVVAEALDYLQRGF
                     ENHYAVSDGATDEGILVGDNAYAWAVETIACLDEPMFVAVASRMIRDGVGRIAAGERV
                     TLELWMPHLAGLLDVISGEGQERSEARIREAAEEARNPYG"
     gene            3225..3953
                     /locus_tag="VFT03_11000"
     CDS             3225..3953
                     /locus_tag="VFT03_11000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848717.1"
                     /translation="MGDLREKRLPAGTERARLVREMFDRIAGRYELLNTVMTAGLNRL
                     WNREVVRVSGLQPGDRALDLACGTGSLTRDLARRVGTKGYVLGVDFSREMLRVARARP
                     APNVEYRIGDATDLEGIPSGAFDAATIAYGARNIPDLDALFSEMARSLRPGGVAVCLE
                     IARPTNRVFGAFYGLWFDRVVPRLGARISGDAWAYSYLPESVKEFVAPDELSDIMERN
                     GLQDVTWRRLAGGIITLHRATRRP"
     gene            3877..4920
                     /locus_tag="VFT03_11005"
     CDS             3877..4920
                     /locus_tag="VFT03_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU4848718.1"
                     /translation="MDYKMLHGGGSRAASSPSTAQRGDPEAGLPSALPVGAFETVDEV
                     EALLGGVGERAPAALAGPAHEALTSGGKRLRPLLLVLCARIGEPERGDLLRAATAIEV
                     LHTATLIHDDIVDKAESRRGEPTTVARYGREAAIATGDYLFAEAFSELAKTGDPRLVR
                     AFSEASEGLAAGELEQYRADGAAVDVEAYLEHIRKKTAGLFKAACVAGGTLGGLSLRQ
                     IDSLATYGQALGIAFQMSDDIMDLVGKPGLMGKGIGTDLAEGTVTLPVIFALREGDAP
                     TIRKVLASPDPSPELLEAGIEAVLATDAVARTEAWARGEIDAALEGLGLLPECPEREF
                     LEVIASEVVGRDA"
     gene            4913..>5649
                     /locus_tag="VFT03_11010"
     CDS             4913..>5649
                     /locus_tag="VFT03_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4848719.1"
                     /translation="MPEPRPTIFAAGLVLPVSAPPISEGAVLVRDGRLEAIGTLEDLG
                     QQNADAELRYYPHYTIVPGAVNTHAHLGFRRGDAPEGGSFSKWLAGLIERLPEKEKWT
                     AEAARASAREAIEAGTTFMAESSPYGECLPQLAASGMAGTVYAEFFPHEIGTPEEAVE
                     FIIQKVRGLREGLPERVDAQVSVHSPYTVDPESSRLAARRTRELGWRLAIHLSESPEE
                     VEFVREGSGGLTDIFGSNNWGGVGVPPV"
BASE COUNT          971 a         1772 c         2034 g          872 t
ORIGIN      
        1 ggcgactctc tcgcggggaa gaaggttctc atcccgcgcg cgctggtggc gcgggagata
       61 ctgcccgaga agctgcgcga agccggcgcc gaggtcgccg ttccgccggc atacgagtcg
      121 gtcccgtcct ccgaaggcgc agacgggctg gcaaggcggc ttgaggcggg cgaggtggac
      181 tgcgttacct tcacagccag ctcgacagtg gagaacttcg tcggggcgtt cggcgaggaa
      241 gaggcgcggc ggctgctcct ggggacacgg gtcgcctgca tcggtcctat aaccgccgac
      301 accgcgcgca ggcgcggcat tcgggtcgat gtcgaggcaa gagagtacac tatacccggg
      361 ttggtcgagg cggtagtaga tctgttcgcc gccgacccgg caaagagagg tgagtagcga
      421 tgccattccc gatccagagg ctcaggagga cacgtcgcac gggcgccctg cgcggcctgg
      481 tgcgcgagac gcggctctcc ccgagcgacc tcgtctaccc tatcttcgtg acggttggag
      541 aggacgtgaa gaacccggta gcttccatgc cgggcgtctt ccagctctcc ataaaccacg
      601 ccgtcgcaga ggcgaagagg gcgagcgatc tcggggtgcc cgcggtcttg ctcttcggca
      661 tcccgaacca gaaagacgag gcggctagcg gagcgtacga ccccgagggg atagtccagc
      721 tcgcgacccg tgccataaag gacgctgcgc ccgagctgat cgtgatcacc gacgtctgcc
      781 tgtgcgagta cacgagccac ggccattgcg gggtcgtgga aaagggtacg ggcgaggtca
      841 tgaacgatgt gagcctcgag ctcctggcgc gcaccgcctc atcccaggca gaggccgggg
      901 cggacatcgt cgcgccatcc gacatgatgg acgggcgggt agcggcgata cggagcgagc
      961 tggacaacga ggggttccag gacaccccga tcctggccta cgctgccaag tacgcctcgg
     1021 ccttctacgg acctttccgc gaggccgccg agagcgcccc cgccttcggc gaccggcgca
     1081 gctaccagat ggaccccgcc aacgcgcgcg aggctctcct cgaggtcgag ctggatgtcg
     1141 aggaaggcgc ggacatcgtg atggtgaagc ctgcccttcc ttacctcgac gtcgtacgca
     1201 gggtcaggga gacgacgaag ctgcccgtcg ccgcgtacag cgtgagcggg gagtacgcga
     1261 tgatcaaggc tgcctctcag aacggctggt tcgacgagag aacggcggtc ctcgaggcgc
     1321 tcacaggcgt gaagcgcgcc ggcgccgaca tcatcgtcac ttaccacgct ccggatgtag
     1381 cccgctggct ctcggcttag ggtaacctcg ctcaccaccc atatcggcca aacaatataa
     1441 acttcgacca ggcgttacga agggggtttc gcgcgcttga acgagcgagg cggcgacgtg
     1501 gacgcgagga cgatgctcgc gatggaccgt tcggcccggc tcatggagcg ggcactgaga
     1561 aggatgcccg gcggggtgaa cagcccggtt cgggcgttcc gctccgtggg cggagatccc
     1621 ctgttcgcgc agcggggcga ggggtcgcgc atcatcgacg ttgacggcaa ctcctacgtg
     1681 gattacgtga tgagctacgg gccgctggta ctcggccacg cgcatcccga ggtgatcgaa
     1741 gccatagagc gtacagcccg caagggcacg acatttggcg ctccgacaga gctcgaggtc
     1801 gaactcgccg agctggtgtg cgaggccgtc ccctcggtag aggtcgtgcg gatgacgaac
     1861 tccggcacag aggccacgat gagcgccata aggctcgccc gcggcttcac ggaccgcgag
     1921 aagatactca agttcgacgg caactaccac ggccacggcg acgccctgct cgtctccgct
     1981 ggctccggcg tcgccaccct tggcctcccg gatagtcccg gcgtcacgaa gggcgcggct
     2041 agagatacgg ccgtcctgcc gtacaacgaa ctcggcgccg tgcgggagct gttcgagaga
     2101 gagggcgagg ctttcgccgc ggtgatcctg gaacccgtgg ccgggaacat gggttgtgtg
     2161 ccgcccgccg aaggctacct cgaagggttg cgcgagatca cgagcgagta cgcgacgctc
     2221 cttatcttcg acgaggtcat gaccggcttc aggctggcgc gcggcggcgc ccaggaacgc
     2281 ttcggtgtta cgccggacct cacgacctta ggcaagataa tcggtggggg actccctgtc
     2341 ggtgcgtatg gcgggaggcg cgagatcatg gatctcgtcg cgcccacggg tccggtctac
     2401 caggccggca ccctctctgg taacccgctc gcgatggccg cgggtctcgc cacgctgcgc
     2461 gccaccggcg aaccgggctt ctacgagcgg ctcgaagagc tcggacgacg ctggcgccag
     2521 gggatgaatg aagctgcctc cgcgggcggc gtcccgttcg cgataaacca ggtcggctcg
     2581 atggcgagca tcttcttcac gggagggccg gtcaccgact tcgccgccgc cgcgtcttcg
     2641 gacgcagagg ctttcaagga cttcttctgg cacatgatct ctcgcggcgt ctacctggcg
     2701 ccgagccagt acgaggccgg ctttatctcg accgcgcact cggagcggga cctcgagatg
     2761 acctgcgagg cggccggcga gtggttcgca aggggtgtct gaggtggaga cggcccgccg
     2821 gacgccagag gcttcgctta aagagatctt cctcatggcc gcctcgcgtc tgccgggtga
     2881 ggacgacagg gccggggtcg tcgccgaggc gctcgactac ctgcagcggg gtttcgagaa
     2941 ccattacgcc gtcagcgacg gagcgacgga cgaggggatc ctggtcgggg acaacgccta
     3001 tgcgtgggcc gtggagacca tagcgtgcct cgacgagccc atgttcgtgg ctgttgcgag
     3061 ccgcatgatc cgggacggcg tgggtcgtat cgccgcgggc gaaagggtca ccctagagct
     3121 ctggatgccg cacctggcag gtctccttga cgtcatatcc ggtgagggcc aggagaggtc
     3181 cgaggcacgc atccgcgagg cggcggagga agcgcggaac ccttatgggt gacctccgcg
     3241 agaagcggct gccagcgggg acggagcgag cgcggctggt gagggagatg ttcgaccgga
     3301 tcgccggccg ctacgagttg ctgaacacgg tgatgaccgc agggctcaac cgtctctgga
     3361 acagggaagt ggtcagggtg agcggtctgc agcccggcga cagagccctc gacctggcgt
     3421 gcggcacggg gagcctcacg cgcgacctcg ccagaagggt agggacgaag ggatacgtgc
     3481 ttggggtaga cttctcgaga gagatgttgc gggtggcgcg ggcgcgtccc gcgcctaacg
     3541 tcgagtaccg gatcggcgac gcgacggacc tggagggcat cccttccggg gccttcgacg
     3601 cggcgaccat cgcctacggc gcgaggaaca tccccgacct cgacgcgctc ttctccgaga
     3661 tggcccggag cctgagaccg ggcggcgtgg cggtgtgcct ggagatagcc aggccaacca
     3721 atcgcgtctt cggcgcgttc tacgggttgt ggtttgatag ggtcgtgccg cggctagggg
     3781 ccagaatctc cggcgacgcc tgggcgtact cctacctacc cgaatcggtg aaagagttcg
     3841 tggcgcccga cgagttgtct gacataatgg agcgtaatgg attacaagat gttacatggc
     3901 ggcggctcgc gggcggcatc atcaccctcc accgcgcaac gcggcgaccc tgaggcaggt
     3961 ctaccctccg cgctgcccgt tggcgccttc gagaccgtag acgaggtcga agcgctgctc
     4021 ggcggggtgg gagagcgggc ccctgcggcg ctcgccgggc cggcgcacga ggccctcacg
     4081 tcgggaggga agcgcctcag gccgctgctc ctggtgttgt gcgcgcggat aggagagccg
     4141 gagcgcgggg acctgctgcg ggccgcgacc gccatcgagg tgctgcacac ggcgacgctc
     4201 atccacgacg acatagtgga caaggccgag agcaggcggg gggagccgac gaccgtggcc
     4261 cggtacggac gggaagcggc gatcgccacg ggcgactacc tcttcgccga ggccttctcc
     4321 gagctggcaa agacaggtga tccgcggctg gtgcgcgcct tctccgaggc ttccgagggg
     4381 ctcgccgccg gagagctcga gcagtacagg gcggacgggg cggccgtgga cgtcgaggcg
     4441 tacctggagc acattaggaa gaagacggcg ggcctgttca aagccgcgtg cgtggccggc
     4501 ggcacccttg gcgggctctc gctcaggcag atagactcgc tggcgaccta cgggcaggcg
     4561 ctcgggatag ctttccagat gtccgacgat attatggacc tggtaggaaa gccgggcctg
     4621 atggggaaag gaatcgggac ggacctcgcc gagggcactg tgacgttgcc tgtcatcttc
     4681 gcgctcaggg agggggatgc gccgacgatc cggaaggttc tcgcctcgcc tgacccctcg
     4741 ccggagctgc tcgaggccgg catagaggcg gtgctcgcca ccgacgccgt cgccaggacc
     4801 gaggcgtggg ccaggggcga gatagacgca gcgctcgaag ggctcgggct gctgccagag
     4861 tgtcccgagc gcgagtttct cgaggttata gcctccgaag tggtcggaag ggatgcctga
     4921 gccccggccc accatcttcg cggcaggact cgtcttgccc gtctccgcgc cacccataag
     4981 tgagggcgcc gttctcgtca gggacgggcg cctagaggcc atcgggacgc tcgaggatct
     5041 cggccagcag aacgcggatg ccgagctgcg gtattacccc cactacacta tcgttcccgg
     5101 ggcggtgaac acccacgccc acctgggctt ccgccggggg gacgcgccgg agggcgggtc
     5161 gttctcgaag tggctggcgg gcctgatcga acgcctcccg gagaaggaga agtggacggc
     5221 cgaagccgcc cgcgcctcgg cccgcgaggc tatcgaggcg gggaccacgt tcatggccga
     5281 gtcctcgccc tacggcgagt gcctgccgca gctcgcagcg tccggtatgg ccgggaccgt
     5341 ctacgccgag ttcttcccgc acgagatcgg cacgcccgag gaagcggtcg agttcataat
     5401 ccagaaggtc aggggactgc gcgaaggtct gcccgagagg gtagatgctc aggtcagcgt
     5461 ccactccccg tatacggtcg accccgagtc gtcgcgcctg gcggcgcggc ggacgcggga
     5521 actcggctgg cggctcgcga ttcacctctc cgagagcccc gaggaggtgg agttcgtcag
     5581 ggaagggagc ggcggcctca cagacatctt cggcagcaac aactggggag gcgtgggagt
     5641 gccgcccgt
//
LOCUS       DASTUV010000546         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1270381, whole genome shotgun sequence.
ACCESSION   DASTUV010000546 DASTUV010000000
VERSION     DASTUV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270381"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFT03_11015"
     CDS             <1..1115
                     /locus_tag="VFT03_11015"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEU4848720.1"
                     /translation="DGLLRRYLEDEGRFPETVGIVVWGTAAMRTQGDDVAEILALLGV
                     RPIWNQESLRVTGIEIIPPEELGRPRIDVTVRISGFFRDAFPNLISLMDDAVRAVADL
                     DEPHDVNFVRKHAEEEKKAGADGRRSTTRIFGSKPGAYGAGLLPLIDARNWRDDEDLA
                     EVYAVWGGYAYGKDLNGVEAREAMESNLRRTEVAVKNVDNREHDLFDSDDYFQYHGGM
                     IAAVRALTGRSPKGFIGDSADPSRPRTRSLSEEARRVFRSRVANPKWIEAMQRHGYKG
                     AFELSATVDYLFGYDATAGVVEGWMYRDVTQKYVLDEKVRDFMQESNPWALRAIAERL
                     LEAAERGLWSEPDPEDLEALKAAYLENEGMLEEGPA"
     gene            1112..2269
                     /locus_tag="VFT03_11020"
     CDS             1112..2269
                     /locus_tag="VFT03_11020"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4848721.1"
                     /translation="MSAPYPFSAIVGQENLKLALLLNAISPEVGGVLVRGEKGTAKST
                     AVRALARLLPPIRVISGCPYSCDPESQNPECPAGPHPEDASWEWRPVRLAELPVGAST
                     DRLTGTLDIERALTQGEKAFEPGILASAHRGILYVDEVNLLSDHLVDLLLDVAAMGVN
                     HVEREGVSVRHPSRFILVGTMNPEEGELRPQLLDRFGVTVEVSGNLDMDERVEIVRRR
                     LRYESDPEDFATEWAAADRELSRSIEEARGGLPAVHLPEETLHKIAAVCTHLGVDGLR
                     GDLVTAKAARALAAWEARGVVATEDVRRAALLALPHRRRRSPFEATGIDPEELESLLP
                     DDPDPDPPGAGSPPPDGTGGRGSTPEEPSETRSGFGERTFSATEGFEPARL"
     gene            2270..3034
                     /locus_tag="VFT03_11025"
     CDS             2270..3034
                     /locus_tag="VFT03_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU4848722.1"
                     /translation="MPEKGSGGPLGRRSRVVGDHGHPVGDREAGETSEDIALAATVRS
                     AAPHQRARGRDEAGLVLRREDLRTNLREGREGNLVLFVVDASGSMAARSRMAAVKGAV
                     LSLLTDAYQRRDKVGLVSFRGEGARVLLPPTSGVELAASRLADLPTGGRTPLAAGLEK
                     AAEILMRERTREKERRPLILVLTDGRTTAGPDPRAAAAALRRLGISSVVVDTEEGYVR
                     LGMAAALAEALGARCLRLEELRADALVELVERRRVA"
     gene            3031..3603
                     /gene="cobO"
                     /locus_tag="VFT03_11030"
     CDS             3031..3603
                     /gene="cobO"
                     /locus_tag="VFT03_11030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU4848723.1"
                     /translation="MSEVREPRERRLRRRSNRERPLLIVNTGHGKGKSTAAFGVMLRG
                     WARGYRVGVYQFVKSGKWNVGEQRAAETLGNIDWFKMGDGWTWTSKDLVESADRAREG
                     WEEVKRRLADETYDMLLLDEFTYPMKFGWLDTQEVVDVLENRPGFQHVIITGRDAPQE
                     LVEIADLVSEVSKVKHPFDEGYRGQKGIEW"
     gene            3597..4952
                     /locus_tag="VFT03_11035"
     CDS             3597..4952
                     /locus_tag="VFT03_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEU4848724.1"
                     /translation="MVETPRIVVAGTHSGAGKTTVASGLMAALSRKGLGVAPFKVGPD
                     FIDPSYHALASGRPGRNLDAFLSGSELIARLFAHGSRGADVAVVEGVMGLFDGKSGAG
                     EYASTAHVAKLLEAPVLLVIDASAMARSVAAMVHGYATFDPEVRVAGVILNRVGSPTH
                     ESMLREAIASLNIPVLGVLRRHAHIHTPYRHLGLVPASERRDEARAALDAATATISRS
                     CDLDAILRLAGSAGRLDAEPWSPEAPDHGPQARVAVASGPSFSFLYEENLELLCGAGA
                     ELVFFDPTSDEDLPGAADALYLGGGFPETYAESLAANRSLKQQVRRFAAEGRPIVAEC
                     GGLLYLIEELDGHRMCGILDARALMTDRLTLGYREARSPHDSPLAEAGTRVRGHEFHY
                     SAVEPGAGERPAWELEGRGPEGFVSGGVHASYLHLHWAATPEIPHRLVRAAVESAGVR
                     A"
     gene            4949..5662
                     /gene="cobI"
                     /locus_tag="VFT03_11040"
     CDS             4949..5662
                     /gene="cobI"
                     /locus_tag="VFT03_11040"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEU4848725.1"
                     /translation="MSGRLIGVGVGPGDPELLTLKGLRALQQADHVFVPVGDTGEIGR
                     AERVVQAHLHPYRARRLLFALSDDASARAENWERGAQEVSQPLLRGETCAFATIGDPN
                     VYSTFTYLARKVRQYIPEAYVETVPGITAMQDLAARGGAVLLEGEERLALLSFTAGEN
                     ALRSALSGFETVVCYKGGSRLREVLEIAAETGRLEDAIYGSRLGMEGEEVLQAREVVG
                     REGPYLATLIFTARGREFG"
     gene            5649..>5862
                     /locus_tag="VFT03_11045"
     CDS             5649..>5862
                     /locus_tag="VFT03_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4848726.1"
                     /translation="MNLGKVWFIGAGPGAPDLLTVRGARLIGEADVVIWARSLVNEGV
                     LEHASPDAEIVESTTIPLESVRELYER"
BASE COUNT          975 a         1930 c         2075 g          882 t
ORIGIN      
        1 cggacggcct cctgcggcgt tatctggagg atgagggacg gtttcccgag acggtgggga
       61 tcgtggtgtg gggcacagcg gcgatgcgca cccagggcga cgacgtggct gagatacttg
      121 cgcttctagg cgtgagaccc atctggaacc aggagtcgct gcgggtgacg ggtatcgaga
      181 taatcccgcc ggaagagctc ggacgtccca ggatagacgt cacggtgcgt ataagcggct
      241 tcttcaggga cgcttttccg aacctgatct ccctgatgga cgacgccgtc cgcgccgtag
      301 cggacctgga cgagcctcac gacgtgaatt tcgtcaggaa gcacgccgag gaagagaaga
      361 aagcgggcgc ggacgggcgc cgctctacga cgcgcatctt cggctcgaag ccgggcgcct
      421 acggggccgg tctcctgccg ctcatagacg cccgcaactg gcgcgacgac gaggacctcg
      481 ccgaggtcta cgccgtatgg ggcggctacg cctacggtaa ggacctgaac ggcgttgagg
      541 cgcgggaagc gatggaatcc aacctgcggc gcacagaggt cgcggtaaag aacgtggaca
      601 accgcgagca tgacctcttc gactccgacg actacttcca gtaccacggg ggcatgatcg
      661 ccgccgtccg cgccctcacg ggccgcagcc cgaaagggtt tataggggac tcggcagatc
      721 cctcacgccc gaggacgcgg agcctctccg aagaagcccg ccgcgtgttt cgctcgcgcg
      781 tcgctaaccc gaagtggata gaggccatgc agcgtcacgg ctacaagggt gccttcgagc
      841 tctccgccac cgttgactac ctcttcggct acgacgccac cgctggcgtg gtcgaaggct
      901 ggatgtaccg cgacgtgacc cagaagtacg tcctggacga gaaggtgcgg gacttcatgc
      961 aggaatccaa tccctgggcc ctgcgcgcca tcgccgagcg gcttctggaa gccgcagagc
     1021 gcggcctgtg gtcagagccg gaccccgaag acctcgaagc gctaaaggcg gcgtacctgg
     1081 agaacgaagg gatgctggag gagggacccg cgtgagcgct ccgtacccct tctccgccat
     1141 cgtcggccag gagaacctga agctagccct gctcctgaac gccatctcac ctgaggtcgg
     1201 cggcgtcctc gtgcggggag agaagggtac cgcgaagtcc accgccgtgc gcgcgctcgc
     1261 gcgcctcctt ccgcccatac gggtcatctc cggctgcccg tactcctgcg accccgagag
     1321 ccagaacccg gagtgtcccg cggggcctca ccccgaggac gcttcctggg agtggcgtcc
     1381 ggtgcggctc gcggagctgc cggtgggggc gagcacggac aggctcactg ggaccctgga
     1441 tatcgagagg gcgctaaccc agggtgagaa ggcattcgag ccggggatcc tggcttcggc
     1501 gcacaggggc atcctctacg tcgacgaggt gaacctgctc tccgaccacc tggtggacct
     1561 actgctcgac gtggcggcta tgggcgtgaa ccacgtcgag cgcgagggcg tgagcgtgcg
     1621 tcacccttcg cgctttatcc tggtcgggac gatgaacccg gaggagggag agctgaggcc
     1681 gcagctcctg gaccggttcg gcgtcacggt ggaggtctcg ggcaacctcg acatggacga
     1741 gagggtcgag atcgtccgcc gccgcctccg ctacgagtcc gaccccgagg acttcgcgac
     1801 ggaatgggcc gccgcggaca gggagctgtc ccgtagcatc gaggaggccc gcggagggct
     1861 ccccgccgtc catctccccg aagagacgct ccacaagata gccgccgtgt gtacccacct
     1921 cggggtggac ggcctgcgcg gcgacctggt taccgcgaaa gccgcacgcg ctctggccgc
     1981 gtgggaggcg cgcggcgtcg ttgccaccga ggacgtgcgc cgcgccgcgt tgctcgctct
     2041 cccgcaccgc cggcggcgca gccccttcga ggcgacaggc atagaccccg aggagctgga
     2101 gagcctcctc cccgatgacc ccgacccgga cccgcccgga gccggcagcc ctccgcccga
     2161 cggcaccgga ggtcgcggtt ccacgcccga ggagccgtcc gagacgagat ccggtttcgg
     2221 ggagcggacg ttctcggcta cggaggggtt cgagccggcg cggctctagg tgccggagaa
     2281 gggcagcggc ggacctctcg gtcgccggag ccgggtggtg ggggaccacg gccacccggt
     2341 aggcgaccgc gaggccggcg aaacctccga ggacatcgcg ctcgccgcca ccgtgaggtc
     2401 ggcggccccg caccagcggg cgcgcggtcg agacgaggca ggcctcgtcc tgcgccgcga
     2461 ggacctgcgc acgaacttgc gcgagggccg cgagggcaac ctcgtcctct tcgtggtgga
     2521 cgcgagcggc tcgatggccg cgcgtagccg gatggccgcc gtgaagggag ccgtgctctc
     2581 gttgctgacc gacgcctacc agcggcgcga caaagttggc ctcgtctcgt tcaggggcga
     2641 gggggccagg gtgctcctgc caccgacctc gggcgtggag ctggcggcct cccgcctcgc
     2701 agatctcccg acgggcggcc gcacacctct ggcggcgggc ctggagaagg cggcggagat
     2761 cctgatgcgc gaacgaactc gggagaagga gcgtcgtccc ctgatcctgg tcctcaccga
     2821 cggaaggact acggccggac ccgacccgcg cgccgccgcg gcagcgttac ggcggctcgg
     2881 gatctcctct gtcgtggtgg acactgagga gggatacgtg aggctcggga tggccgcggc
     2941 gctcgccgag gcgctcgggg cgcggtgcct acgcctggag gagctgcggg cggacgcgct
     3001 tgtcgagctg gtggagagga ggcgggtggc gtgagcgagg tgcgagagcc gcgcgagagg
     3061 cggctgcggc gcaggtcgaa cagggagcgc ccgctcctga tcgtgaacac agggcacggc
     3121 aagggcaaga gcacggcggc gttcggggtc atgctcaggg gctgggcccg cggctaccgt
     3181 gtcggcgtct accagttcgt caagagcggc aagtggaacg tcggagaaca gagagccgcc
     3241 gagactcttg gcaacataga ctggttcaag atgggggacg gctggacgtg gacatcgaag
     3301 gacctcgtcg agagcgcgga cagggcgcgc gagggttggg aggaggtcaa gcggcggctc
     3361 gcggatgaga cctacgacat gctcctcctc gacgagttca cctacccgat gaagttcggc
     3421 tggctggaca cgcaagaggt ggtcgatgtc ctcgagaacc ggccgggttt ccagcacgtc
     3481 atcatcacgg gccgcgacgc gccgcaggag ctcgtcgaga tcgccgacct ggtgagcgag
     3541 gtcagcaagg tcaagcaccc cttcgacgag ggctaccgcg ggcagaaggg catcgaatgg
     3601 tagagacccc tcgcatcgtc gtcgctggca cccactcggg ggcgggcaag accaccgtcg
     3661 cgtccggtct gatggccgct ttgtcccgca aaggccttgg cgtcgccccc ttcaaggtcg
     3721 gccccgactt catagacccc tcctaccacg ccctcgcttc cggccgccca ggccgtaacc
     3781 tcgacgcctt cctctccggc tccgagctca ttgcccggct cttcgcccac ggctcgcgcg
     3841 gcgcagatgt cgcggtggtg gaaggtgtga tgggcctctt cgacggcaag agcggggccg
     3901 gggagtacgc ctcgactgcc cacgtcgcga agctcctcga agccccggtc ctcctcgtga
     3961 tagacgcctc ggcgatggcg cggtctgtcg cagccatggt gcacgggtac gcgaccttcg
     4021 acccggaggt acgggtcgct ggtgtgatcc tgaaccgcgt cggctccccg acccacgaaa
     4081 gcatgctccg cgaagctata gcctccctga acatccccgt cctcggcgtc ctgcgccgcc
     4141 acgctcacat ccacacgcca tataggcacc tcggcctggt gcccgcctcc gagcgccgcg
     4201 acgaagcccg cgcagccctc gacgccgcaa cagcgacgat ctcccgttcc tgcgacctcg
     4261 acgccatcct gcgcctcgcc ggctccgccg gacgcctgga cgcggagccc tggagcccgg
     4321 aagcccccga tcacggacca caggcgcgcg tcgcggtagc gtctggcccc tcgttcagct
     4381 tcctctacga agagaacctg gagctgctgt gcggagcggg cgccgagctc gtcttcttcg
     4441 accccacctc cgacgaggat ctccccgggg ccgccgacgc cctctacctc ggcggcggtt
     4501 tccccgagac ctacgccgaa tctctcgctg cgaacagatc actgaaacaa caggtgcgcc
     4561 ggttcgcggc ggagggaagg cccatcgtcg ccgagtgcgg cggcttgctg tacctgatcg
     4621 aggagctgga cggccaccgg atgtgcggca tactcgacgc gagagccctg atgacggacc
     4681 gcctcactct aggttatcgt gaggcgcgct ccccccacga ctcgccgctc gccgaggccg
     4741 gaacccgcgt gcgcggccac gagttccact actcggcggt cgagccgggg gcaggggaga
     4801 ggccagcatg ggagctcgag ggccgaggac cggagggttt cgtctcgggc ggcgtgcacg
     4861 cgagctacct gcacctgcac tgggcggcga cgccggagat cccgcaccgt ctcgtgcgag
     4921 ccgccgtcga gagcgcaggg gtgagggcgt gagcgggcgc ctgatcgggg tcggcgtagg
     4981 acccggagac cctgagctcc tgacgctgaa gggcctgcgc gcgcttcaac aggcagacca
     5041 cgtcttcgtc cccgtcggcg acaccggcga gatcggccgc gccgaaagag tcgtgcaggc
     5101 ccacctccac ccctaccgcg cccggcgctt gctattcgcc ctctccgacg atgcctccgc
     5161 ccgcgccgag aattgggaac ggggtgccca agaggtctca caacccctcc ttcgcggtga
     5221 gacctgcgcc ttcgccacca tcggagatcc caacgtgtac tccactttca cgtacctggc
     5281 ccgtaaggtg aggcagtata tacctgaagc ttatgtagag actgtaccgg ggatcacggc
     5341 gatgcaggac ctcgccgcgc ggggtggtgc ggtgctgctc gagggtgaag agcggcttgc
     5401 cctcctatcc tttaccgcgg gagaaaacgc tttacgtagt gcgttatccg gcttcgagac
     5461 ggtcgtgtgc tacaaaggcg ggagccggct gcgggaggtg ctggagatcg ctgccgagac
     5521 cggaaggctc gaagatgcaa tctacggttc acggttgggc atggaggggg aagaagtgtt
     5581 gcaggcgcgt gaggtagtgg gacgggaggg gccttatttg gcgactttaa tcttcacggc
     5641 gagggggcgt gaatttgggt aaggtgtggt ttatcggagc ggggcctggg gcgccggatt
     5701 tgctgaccgt gcgtggggcg cggctcatag gtgaggcgga cgtcgtgatc tgggcgagga
     5761 gcctggtgaa cgagggggtt ctggagcacg cgagcccgga cgcggagatc gtcgagtcca
     5821 ccacgatccc cctcgaaagc gtacgcgagc tctacgagag gg
//
LOCUS       DASTUV010000547         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_240673, whole genome shotgun sequence.
ACCESSION   DASTUV010000547 DASTUV010000000
VERSION     DASTUV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240673"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..604)
                     /locus_tag="VFT03_11050"
     CDS             complement(17..604)
                     /locus_tag="VFT03_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEU4848727.1"
                     /translation="MGKGNEKVGRFLRVLKVNPVIPAVRGRDGDLERALAGDHAAIFV
                     LGGDVFRMLERVRRRPYRRPFICVNVDMVGGVAPDPSGIRFLAREVDGVISTHRSVVQ
                     IARSNGALAIQRLFAIDTSAVERGLKVLRQADPDAVEILPGLAFPEIVESYRAALNKP
                     VLAGGLIKDRATAATILRAGAVGVSTSDHRLWKLA"
     gene            671..1705
                     /gene="selD"
                     /locus_tag="VFT03_11055"
     CDS             671..1705
                     /gene="selD"
                     /locus_tag="VFT03_11055"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:722..724,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU4848728.1"
                     /translation="MDERTRIRLTKYSTKAGUASKFAPGDLEQALKGLAPTAVDGFVS
                     GMETRDDAGYVPFGGGLLLQTADFFTPIVDDPYRFGQIAAANAFSDVYAMGGEPLTAL
                     NLVAFPCSLDLGVLREILAGGQSKIEEAGARLCGGHSVQDEEPKYGLSVTGWVEEERV
                     VRNAGAKVADALVLTKPLGLGILTTALKRDLVSEAEIEEAVSTASHLNRGARDAVRDV
                     GVSAATDVTGYGFLGHLFEMLEASGVGAVVRRSEVPVWELAVPLANDGCYPGGLKSNR
                     EYLEGRVFADGVGPDELLPLYDPQTSGGLLVAVPEERASAFISSLEERGVSGVVVGEI
                     VEGRGVRVAP"
     gene            1802..>2034
                     /locus_tag="VFT03_11060"
     CDS             1802..>2034
                     /locus_tag="VFT03_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU4848729.1"
                     /translation="MQIILNTALPFFALIFCGYAAGRFNVLSEASISGVNAFVFYFAL
                     PTFLFNLMATSPIGEVPIGSFVAAYLGTGLAVFA"
BASE COUNT          319 a          694 c          664 g          357 t
ORIGIN      
        1 tccgccggat acggagtcaa gcgagcttcc agagcctgtg gtcgctcgtg gagaccccga
       61 cggcgccggc cctgaggatg gtggcggcgg ttgcgcggtc tttgatcagg ccgcccgcca
      121 acaccggctt gttgagggcg gcccgatagc tctctactat ctccgggaag gcgaggccgg
      181 gaagaatctc gacggcgtcg gggtcggcct ggcgcagcac cttcagcccc cgctcgacgg
      241 cgctggtgtc tatggcgaag agacgctgga tcgctagcgc cccgttgctc ctggcgatct
      301 ggacgacgct gcggtgggta gagattacgc cgtccacctc cctggcgagg aaccggatgc
      361 cgctcgggtc cggcgccacc ccgccgacca tatccacgtt gacgcagatg aacggccgtc
      421 tgtagggcct cctcctcacc ctctcgagca tccggaacac gtcccccccg agcacgaaga
      481 tcgccgcgtg gtcccccgcg agcgcccgct cgagatcccc atcccgcccc cggacagccg
      541 gtatgaccgg gttgaccttc agcacccgca gaaagcgccc gaccttctca ttaccctttc
      601 ccatgcgatg actctagcat ctattcgcgc ttaccgatgc cccaaccggc ggcgggtata
      661 ctcagacccg atggatgagc gtacacgcat aaggctgacg aagtactcga cgaaggcggg
      721 ttgagcgtcc aagttcgctc caggggacct ggagcaagcc ctcaagggcc tcgccccgac
      781 cgccgtggac gggttcgtga gcgggatgga gacccgggac gacgccgggt acgtcccgtt
      841 cggcggcggc ctgctccttc aaacggcgga cttcttcacc cccatcgtcg acgatccgta
      901 ccgcttcggc cagatcgccg cggccaacgc cttctccgac gtctacgcga tgggcggcga
      961 gccgctcacc gcgctgaacc tggtcgcctt tccctgctcg ctggacctcg gtgttctgcg
     1021 ggagatcctg gccggcggac agtcgaagat cgaggaggcc ggcgctcgcc tctgcggcgg
     1081 ccactcggtg caggacgaag agccgaagta cggcctctcc gtcacagggt gggtcgagga
     1141 agagagagtc gtgaggaacg ccggagctaa ggtagctgac gcgctggtgc tcaccaagcc
     1201 gctcggcctc ggcatcctta cgacggctct caagcgcgac ctggtctcgg aggcagagat
     1261 cgaggaggcc gtctcgacgg cctcccacct gaacaggggc gcccgcgacg ccgtgcgcga
     1321 tgtcggggtc tcggccgcga cggacgtcac cggctacggc ttcctcggac acctcttcga
     1381 gatgctcgag gcctcgggcg tcggggccgt cgtgcgccgg agcgaggttc cggtctggga
     1441 gctggccgtg cctctggcga acgacgggtg ctatcccggc ggtttgaaga gcaacagaga
     1501 atatctggaa ggacgcgtat tcgctgacgg ggtcgggccg gacgagctcc tgccgctcta
     1561 cgacccgcag acgagtggcg gcctgcttgt cgcggtgccc gaggagcgag cctctgcgtt
     1621 catcagttcc ctggaggagc gcggagtctc cggcgtcgtg gtcggagaga tcgtcgaggg
     1681 acgcggggtg cgggtcgctc catagcgata acctggcgcc acggagactc tggtcccgtg
     1741 gtgtgatact cattggcctc gtgacccgcg gatcaaccag acctgatagc tgagggctgc
     1801 cgtgcagatc atcctgaaca cggcactgcc cttcttcgcc ctgatcttct gcggctacgc
     1861 cgccggacgc tttaacgtcc tctccgaggc gagcatctcg ggcgtgaacg ccttcgtctt
     1921 ctacttcgct cttccgacgt tcctgttcaa cctgatggcg acctcaccga tcggcgaggt
     1981 ccccatcggc tccttcgtcg ccgcctacct cggcacgggc ctcgccgtct tcgc
//
LOCUS       DASTUV010000548         5914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_155295, whole genome shotgun sequence.
ACCESSION   DASTUV010000548 DASTUV010000000
VERSION     DASTUV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155295"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..917)
                     /gene="pyrF"
                     /locus_tag="VFT03_11065"
     CDS             complement(78..917)
                     /gene="pyrF"
                     /locus_tag="VFT03_11065"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU4848730.1"
                     /translation="MRALVEPARRKGSALVVGLDPIPSRFPKELRGLPQAHASQEFCR
                     GILEAVEPYAAAIKLQAAYFERLGPPGMEVYGDLIADATALGLPAIADVKRGDIGSVA
                     AAYAEAHLAVYGAACVTVNPFMGADAVAPFLEETRRQARGGGVFALVATSNPSAESIQ
                     AATHPPLFEVSARLVAELGSGEGDYPDAGAVVGATRPETGRRVRELLPDALFLVPGYG
                     AQGGEASGIRPLLDRRDEGVLVNSSRGVLYAYEGLRTGYREAAREAAKRARDELRAAG
                     VAT"
     gene            complement(1007..1279)
                     /locus_tag="VFT03_11070"
     CDS             complement(1007..1279)
                     /locus_tag="VFT03_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4848731.1"
                     /translation="MNKTELIEKIADETNGSKNEAQRFFDAFTTVVESELKRGNQVQI
                     TGFGKFYVQQRDARQGINPQTKQRINIPASKVPKFTAGNALKDSIK"
     gene            complement(1361..1954)
                     /gene="pyrE"
                     /locus_tag="VFT03_11075"
     CDS             complement(1361..1954)
                     /gene="pyrE"
                     /locus_tag="VFT03_11075"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU4848732.1"
                     /translation="MGWTSGPSTRHWTGSSGGSASLVPPDLASRVREAALLEGDFVLS
                     SGKRSSFYVDKYLFSTQPGLLRDLADALSTKLPDGVERLAGVELGAVPLVVATALVTG
                     LPYVIVRKSAKDHGSSAGRDVEGNLESGERVALIEDVVTTGTQALRAAESLAREGADV
                     VTIVAVLDRREVSEPSLGGFPFQALLKLEDLRVGKKD"
     gene            complement(1878..2162)
                     /locus_tag="VFT03_11080"
     CDS             complement(1878..2162)
                     /locus_tag="VFT03_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848733.1"
                     /translation="MEDRTPDIEGLEEKVGELERLADTLDGVPDEELGGELGRAVELL
                     REINTCIEAELDDAARESREIGEIIDGLDLGPFDEALDGLERRERESGTP"
     gene            complement(2217..2477)
                     /locus_tag="VFT03_11085"
     CDS             complement(2217..2477)
                     /locus_tag="VFT03_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU4848734.1"
                     /translation="MQRENKTEVIRRLKSVEGHVRGVAKMVEEGEVSYAEIVQQTNAI
                     FSAIRRINNLLLKDFVEERTSMDGASEELVKDIMKAIERVTK"
     gene            complement(2485..4005)
                     /locus_tag="VFT03_11090"
     CDS             complement(2485..4005)
                     /locus_tag="VFT03_11090"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU4848735.1"
                     /translation="MSLAAYPRRALATLRAVILDRAELARVLGTEIPPGVDEGVRGVA
                     HDSRRVKPGFAFVAIPGFKRDGAEFAPEALRRGASLVVAEREVPEVPTAVVPDARIAL
                     AALARAVFGDPSGSMQVYGVTGTNGKTTTAYALHSILAGAYGEGMCGLMTTVETISAG
                     ERRPAVRTTPEAVEVQETLARMLEAGVRRVVMEVSSHGIELGRVSGTRFEGALFTNLT
                     RDHLDLHGTMEAYYATKRELFRWTESPKVANAEDAWGRRLAREVACVKTFGGTAEADY
                     RVARVETSRNGTSFTLCHSGGDLGLETPLLGPYNVLNVAGAVSLALEAGVEGDAAAGA
                     VRTMGQVPGRFERVAAESGFEVIVDYAHTDVGLEAVLGVARGLVENGRHPGGRVICVF
                     GAAGERDGAKRPRMGRVAARLADLSIVTTDDAYAEDPSRIAREVAVGADPARTEVLLD
                     RRAAIRRALSVARAGDVVIIAGKGHERVQHLPEGDVPFHDATVVGELLEELDEGEG"
     gene            4247..5350
                     /locus_tag="VFT03_11095"
     CDS             4247..5350
                     /locus_tag="VFT03_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4848736.1"
                     /translation="MAAENSMMLAERTDELLNRGRSQGFISTEEVADLVQEGDLSTAE
                     IEEFYAALEEEEITLVESEAPEASGEATTTAPAVKDQETPAIQIAHAVATGDSIRMYL
                     AEIGRVRLLTHADEIRLAKGIARGDKRCKDKLVEANLRLVVSIAKKYRNRGVSFLDLI
                     QEGNLGLIRAAEKFDHCKGFKFSTYATWWIRQAITRAIADKGRTIRIPVHMVEKVNKF
                     HRTHRRMVQFLGREPTDEEIASELRVPVEEILRLQEISQRSISLETPVGDDDSSELGD
                     FLEDAASATPTDAVSESLLKLHLREALDELPERERQIIELRFGMKDDRPRTLEEVGRE
                     FDITRERVRQIQMKTLNLLREQRRTQNLREYLR"
     gene            5334..>5914
                     /locus_tag="VFT03_11100"
     CDS             5334..>5914
                     /locus_tag="VFT03_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEU4848737.1"
                     /translation="MSTSARSSSEELHEFVCDLARRAGELQLSRYEAPGKVTEKAPKD
                     IVTEVDLLCEELMVGAITERYPEDAILSEEQGGEISASGRTWLLDPVDGTANYARANP
                     IFCACVSVLEGGAVTHAAVAAPRIGDLYHAALGGGAYRDSHEGRSRLRVSDTERLEYA
                     FFGADLAFRGVGSSGVRRVLAASWQFRALGSAGV"
BASE COUNT         1022 a         2040 c         1875 g          977 t
ORIGIN      
        1 atccgggcaa gagcccaacg ccagagagta agctgaaatg ctgagaagcg agggccgcaa
       61 ggcccgagcg tgctgactta cgttgcgacg ccggcggcgc gcagctcatc gcgagcccgt
      121 ttcgccgcct cccgcgcagc ctcgcggtaa cctgtccgta atccctcgta ggcgtagagg
      181 acgccccggc tgctgttgac aaggaccccc tcgtcccgac ggtctagcag cgggcgtata
      241 cccgaggcct cgcccccctg agccccgtag ccggggacga ggaagagggc gtcgggcagc
      301 agctcccgca cccgccgtcc ggtctcgggg cgagtggccc cgaccacggc cccggcgtcg
      361 gggtagtcgc cttctccgct cccgagctcc gcgacgaggc gggccgagac ctcgaacagg
      421 ggcgggtgcg tggccgcctg gatgctctcg gcggatgggt tggaggtcgc tacgagcgca
      481 aagacgcccc cgcccctagc ctggcgacgg gtctcttcga ggaacggtgc cacagcgtca
      541 gcccccataa acgggttgac ggtgacgcac gcggcgccgt agacggcgag gtgcgcctcc
      601 gcgtaggctg cggcgacact ccctatgtca ccgcgcttca catcggcaat ggccgggagc
      661 cccagagccg tagcatcggc gatgaggtcc ccgtagacct ccatgccagg tggcccgagg
      721 cgctcgaaat acgccgcctg caacttgata gcggcggcgt aaggctcgac ggcctcgagg
      781 atgccccggc agaactcctg gctcgcgtgg gcctggggca ggccgcgcaa ctccttgggg
      841 aacctagagg ggatgggatc cagtcccacc accagcgcgc tgcccttgcg gcgggccggc
      901 tctacgagcg ctcgcacgat gcggcaccgt cggtcgccag gggggacaag ggactaaagt
      961 gccgcggagg aaacctcccc gcggcacgga ctggatctct ccaggcttac ttgatcgagt
     1021 ctttgagcgc gttgccggcg gtgaacttgg gcaccttgga agcggggatg ttgatcctct
     1081 gcttggtctg cgggttgatc ccctgccggg cgtcgcgctg ctggacgtag aacttgccga
     1141 agccggtgat ctggacctga ttgccccgct tgagctccga ctctaccacg gtggtgaagg
     1201 cgtcgaagaa cctctgggct tcgttcttag agccgttggt ctcgtcggcg atcttctcga
     1261 tcagttccgt cttgttcatc gcttgcttaa cctcccttta ccgcttacac tcctctcccc
     1321 cgaaatcccc gagggaatat accagagacg tgtcagcgcc tcaatccttt ttccctaccc
     1381 gtaaatcctc tagtttaagc agggcctgga aggggaaccc gccgagactc ggctcagaga
     1441 cctcccgtct gtcgagtacc gcaacgatcg ttacgacatc ggccccctct ctcgcgaggc
     1501 tctcggcggc ccgtagagcc tgcgtcccgg tagtcacgac gtcctcgatc agggccaccc
     1561 tttcgccgga ctccaggttg ccctctacgt cgcgtccggc ggagctgccg tggtctttgg
     1621 ccgacttgcg gactatgacg taggggaggc ctgtgaccag agccgtcgcc acaaccaggg
     1681 gcacggcccc gagctcgacg ccggccaggc gctcgacgcc gtcgggaagc ttcgtggaga
     1741 gggcatccgc cagatcgcgg aggaggccgg gctgcgtgga gaagaggtac ttgtcaacgt
     1801 agaagctgct gcgcttcccg ctggagagga cgaaatcccc ctcgagcagc gccgcctcgc
     1861 ggacgcggga ggccaggtca gggggtacca gactcgcgct cccgccgctc gagcccgtcc
     1921 agtgcctcgt cgaagggccc gaggtccagc ccatcgatta tctcgccgat ctcccgcgac
     1981 tccctggcgg cgtcgtcgag ctccgcctct atgcaggtat tgatctccct caagagctcc
     2041 acggccctgc cgagctcccc gcccagctct tcgtcgggaa cgccgtccag cgtgtcggcg
     2101 agcctctcga gctccccgac cttctcctcg agaccttcga tgtccggcgt acggtcctcc
     2161 actggtcccg gactatacat catcctgccg ccggatttgg cctcgagagc cggacactat
     2221 ttcgtcaccc gctctatggc cttcatgatg tccttcacca gctcctctga ggcgccgtcc
     2281 atcgaggtgc gctcctcgac gaagtccttg aggagaaggt tgtttatccg ccggatggcg
     2341 gagaagatgg cgttggtctg ctggacgatc tcggcgtatg atacctcccc ctcctccacc
     2401 atcttcgcga ctccccgcac gtgcccctcc acgctcttca ggcgccttat gacctcggtc
     2461 ttgttctccc gctgcaaacc tctgctaccc ctccccttcg tctagttcct ccagaagctc
     2521 cccgacaacc gtggcgtcgt ggaagggcac gtctccctcg ggcaagtgtt ggaccctctc
     2581 gtgccccttg cctgcgatta tcacgacgtc ccccgcacgc gcgacggaga gagcgcgccg
     2641 gatcgccgcc cgcctgtcga gcagcacctc ggttcgcgcg gggtcggccc ccaccgcgac
     2701 ctctctcgct attctggacg ggtcctcggc gtatgcgtcg tcggtagtga cgatgctcaa
     2761 gtcggcaagg cgggcggcca ccctgcccat cctggggcgt ttggccccgt cccgctcacc
     2821 cgccgccccg aacacgcaga tcacacgccc tcccggatgc cggccgttct ccaccagccc
     2881 gcgggcgacc ccgaggaccg cctctagccc cacatcggtg tgggcgtagt ccacgatgac
     2941 ctcgaatccg ctctctgcgg ccactcgctc gaagcgcccc ggcacctgtc ccatcgtcct
     3001 tacggccccg gccgctgcat ccccctcgac ccccgcctcc agcgcgagcg agaccgcccc
     3061 tgcgacgttg agcacgttgt atggcccgag aagcggggtc tcgagaccga ggtcgccgcc
     3121 tgaatggcag agtgtgaagc tggtcccgtt ccgcgacgtc tctacccgcg cgacgcggta
     3181 gtccgcttcg gcggtcccgc cgaaggtctt gacgcaagcg acctcacgag ccagacgccg
     3241 accccacgcg tcctccgcgt tagctacttt agggctctcg gtccagcgga agagctcgcg
     3301 cttggtcgcg tagtacgcct ccatagtgcc gtgcaggtcg aggtgatccc gggtcaggtt
     3361 ggtgaagagc gccccctcga aacgcgtccc cgagacgcga ccaagctcta tgccgtgcga
     3421 ggagacctcc atgacgaccc gcctgacacc cgcctccagc atccgggcga gcgtctcctg
     3481 tacctcgact gcctcggggg tcgtgcggac agcaggacgg cgctcgccgg cagagatcgt
     3541 ctccacggtg gtcatgaggc cgcacatccc ctcgccgtag gcgccagcga ggatggagtg
     3601 aagcgcgtag gctgtcgtcg tcttgccgtt ggtccccgtc accccgtaga cctgcatgct
     3661 gcccgaaggg tcgccgaaga cggcccgggc gagggcggcg agggcgatcc gggcatccgg
     3721 cacaacggct gtcggtactt cagggacctc acgctcggcg acgacgaggg aggcaccgcg
     3781 gcgcagggcc tcgggggcga actcggcccc gtcgcgcttg aagccgggga tcgcgacgaa
     3841 ggcgaagccg ggctttacgc ggcgcgagtc gtgagcgacg cccctcaccc cttcatctac
     3901 gccgggcggg atctccgtgc cgagaacccg cgcgagctct gcacggtcca ggatcacagc
     3961 ccgtagtgta gcaagggcgc gcctcgggta cgccgcaagg gacaccgcgc gctgatacct
     4021 ccgcgaaatg tccccgaaac aacgccgaaa cacggcgcgg ggcgcaatat atgggcgtcg
     4081 agcgaaagaa aatacacaaa atgggccaaa atcacggtga ctacacatat gtggtgtgct
     4141 aacctacgcc agttaatttt acgggaattt cacaatcgcg atacgtacgg cgatacgtgc
     4201 gcgaagacaa tattgcagcg agagcacggt cgaggagagg agatagatgg cggccgaaaa
     4261 ctccatgatg ctggccgagc gcactgacga gcttttgaat agggggcgaa gccagggctt
     4321 tatcagtacg gaggaggtcg ccgacctcgt acaggagggg gacctctcca cggcagagat
     4381 cgaggagttc tacgccgcgc tcgaggagga ggagatcacg ctcgtagaga gtgaggctcc
     4441 cgaggcctcg ggggaggcga ccacgacggc cccggccgtc aaggaccagg agactccggc
     4501 gattcagatc gcccacgccg tcgccaccgg cgactccatc cggatgtacc ttgcggagat
     4561 agggcgggtg cggctcctca cacacgccga cgagatcagg ctcgccaagg gtatcgcccg
     4621 cggcgacaag cgctgcaagg acaagctagt cgaggcgaac ttgcggctcg tcgtctccat
     4681 agccaagaag taccgcaacc gcggcgtatc tttcctcgac ctcatccagg aagggaacct
     4741 cgggctcatc agggccgcgg agaagttcga ccactgcaag ggcttcaagt tctccaccta
     4801 cgccacctgg tggataaggc aggccataac gcgcgccatc gcggacaagg ggcgcacgat
     4861 ccgcatcccg gtccacatgg tcgagaaggt caacaagttc caccgcaccc accgccgcat
     4921 ggtccagttc ctcgggcgcg agcccacgga cgaggagatc gccagcgaac tcagggtgcc
     4981 tgtagaggag atcctgcgcc tgcaggagat cagccagcgc agcatcagcc tcgagacccc
     5041 tgttggcgac gatgactcat cagagctcgg cgacttcctc gaggacgcag cctcggcgac
     5101 gcctaccgac gccgtctctg agtctctgct caagctgcac ctcagggagg cgctggacga
     5161 gctgcccgag agggagcgcc agatcatcga gctccggttc gggatgaagg acgacaggcc
     5221 ccgcacgctc gaggaagtcg ggcgcgagtt cgacatcacg cgggagaggg tgcgccagat
     5281 ccagatgaag accctgaacc tcctgcgcga gcagcgccgc acccagaacc tgcgtgagta
     5341 cctccgctag gagctcctcc gaagagttac acgagttcgt ttgcgacctg gcccgcaggg
     5401 ccggggagct ccagctctcc cgctacgagg cccctggcaa ggtaacggag aaggccccca
     5461 aagacatagt taccgaggtg gacctcctgt gcgaggagct catggtgggc gccatcacgg
     5521 agcgctaccc cgaagacgct atcctctccg aggagcaagg aggcgagatc tccgcctcgg
     5581 gacgcacctg gctgctcgac cccgtagacg gcaccgcgaa ctacgcgcgc gccaacccta
     5641 tcttctgcgc ctgcgtctcg gtcctcgaag gtggcgctgt cacgcacgcc gccgtcgccg
     5701 cgccccggat cggcgacctc taccacgcgg ctcttggcgg cggagcctac agggattctc
     5761 acgagggccg ctcgcgcctg cgcgtgagcg acaccgagcg gctcgagtac gccttcttcg
     5821 gagcggacct cgctttcagg ggggttggca gttcaggagt gaggcgagtc ctcgccgcct
     5881 cgtggcagtt tcgcgctctg ggcagcgcgg gcgt
//
LOCUS       DASTUV010000549         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1055120, whole genome shotgun sequence.
ACCESSION   DASTUV010000549 DASTUV010000000
VERSION     DASTUV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055120"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFT03_11105"
     CDS             complement(<1..218)
                     /locus_tag="VFT03_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4848738.1"
                     /translation="MPLGGLDEIGKNMTAVRGGSGIILVDAGMAFPDEEMPGIDLVLP
                     DFTYLREHSDEIRGIVLTHGHEDHVGALP"
     gene            complement(329..1588)
                     /locus_tag="VFT03_11110"
     CDS             complement(329..1588)
                     /locus_tag="VFT03_11110"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4848739.1"
                     /translation="MSDPNIGKKELPGGLRVLSEPLEEATSVSLGVWIRAGSRDERDE
                     VAGISHLMEHMLFKGTPDMDALGVAQAFESIGAQENAATGEEYTVLYARFLPEHLERA
                     LDIMSDMVLRPTLADLEREREVIVEEIRMYEDRPDQLADEYLSGLIFHDDPLGRPIIG
                     SAETVRGVDHEILKTFHHGAYTAPNIFVVAAGKLDRDELERMVEEKFSGLPEGQPFTR
                     EARPRPPESRFYYKHKETEQYHVSLGSQGIPARSEDRYAMAALNNVLGGGMSSRLFQE
                     VREKRGLAYAVYSYHQGYSDAGAIKTYVGSTTGNVEEAVRVITEQLGRVQEELVSDEE
                     LDRTKQQLKSSTLLALESTAARMNRIGRSVITGTELLSPEEIAERIEAVSAEDIRRLA
                     RAHLNLDNLYLSAVGPEELDLGRHLNS"
     gene            complement(1592..>2562)
                     /locus_tag="VFT03_11115"
     CDS             complement(1592..>2562)
                     /locus_tag="VFT03_11115"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU4848740.1"
                     /translation="SKRYMHHYNFPPFSTGETGRLGSPRRREIGHGALAERALLPVIP
                     DEESFPYALRIISDVLESNGSSSMASVCGSTLALMDGGVPIKAPVSGVAMGLVKEGDD
                     YVILTDIQGLEDHMGDMDFKVAGTRDGITALQMDMKITGVSAQLLKEALEKAKRGRIE
                     ILDIMRETIAEPRSEVSDYAPRVEVLQIPTDKIGLLIGPGGKTINALQDEYGVNISVE
                     NDGTVFVAGVDGVSVKGALSAINGMTKDVESGDIYTGKVVKTTNFGAFVELMPGRDGL
                     IHISRLAPGKQRVERVEDVVSEGDRVKVRVLDIDKQNRISLEKLEE"
BASE COUNT          401 a          838 c          805 g          518 t
ORIGIN      
        1 gggagagccc ccacgtggtc ctcgtgcccg tgggtgagga ctatcccccg gatctcgtcc
       61 gagtgctccc tcagataggt gaagtccggc aggaccagat ctatccccgg catctcctcg
      121 tcggggaagg ccatcccagc gtcaaccagg atgatgccgc tccccccgcg cacggcagtc
      181 atgttcttgc cgatctcgtc cagcccccca aggggcacga cctgcaacgt tttgtcgtct
      241 agcagttcgg attacctcct cattcaaagg cttcaggtat caggcatcag ggggttttcc
      301 tgaagccttt agcctgaaac ctgaattcct aactgttcag gtgcctcccg agatcgagct
      361 cctcgggccc gacggcggag aggtacaggt tatccaggtt caaatgtgct cttgcgagac
      421 gtcttatgtc ctccgcgctg accgcctcga tgcgctcggc gatctcctcg ggggacagca
      481 gctcggtgcc tgttatgacg ctccgtccga tgcggttcat cctcgcggct gtgctctcta
      541 gggcaagcag cgtcgagctc ttgagctgct gcttggtgcg gtcgagctcc tcgtcggaaa
      601 cgagctcctc ctgcaccctg cctagctgct ccgtgatgac gcgtaccgcc tcctccacgt
      661 tccccgtggt ggagccgacg taggtcttga tggcgcctgc gtcagagtag ccctgatggt
      721 aggagtacac ggcgtaggcg agcccacgct tctcccgcac ctcctggaag agccggctgc
      781 tcatgccgcc gccaagcacg ttgttcaggg ccgccatggc gtagcggtcc tcgctgcggg
      841 ccgggatccc ctgggatccc agagagacat gatactgctc cgtctccttg tgcttgtagt
      901 agaagcggct ctccggtggc cgggggcgcg cctccctcgt gaagggctgg ccttcgggga
      961 ggccagagaa cttctcctcg accatccgct ccagctcgtc ccggtcgagc ttgcctgcgg
     1021 ccacgacgaa gatgttgggg gccgtgtagg caccatggtg gaacgtcttc aatatttcgt
     1081 ggtcaactcc gcggacggtc tcggccgagc cgataattgg gcgtccgaga gggtcgtcgt
     1141 ggaagatcaa gccggagagg tactcgtcgg ccagctggtc aggccggtcc tcgtacatcc
     1201 ggatctcctc gacgatgacc tcgcgctcgc gctcgaggtc ggcgagcgtg ggccgcaaga
     1261 ccatgtcgct catgatgtcg agcgcccgtt ccagatgctc ggggagaaag cgggcgtaga
     1321 gcacggtgta ctcctcgccg gtggcagcgt tctcctgggc accgatgctt tcgaaggcct
     1381 gggcgacccc gagagcatcc atgtccgggg tccccttgaa gagcatgtgc tccatcaggt
     1441 gggagatgcc cgcgacctcg tccctctcgt cgcggctgcc agcccggatc cagacgccca
     1501 gggagacgct cgtcgcctct tcgaggggct cggagagtac ccttaagccc cccggaagct
     1561 ccttcttgcc tatgttggga tcgctcaagg gttactcctc cagcttctcg agggagatgc
     1621 ggttctgctt gtcgatgtcg agcacccgca ccttcacgcg gtcgccctcg ctcacgacgt
     1681 cctcgacgcg ctcgacgcgc tgtttgcccg gcgcgaggcg ggagatgtgg atcagaccgt
     1741 cccgtcccgg catgagctcg acgaaggcgc cgaagttggt ggtcttgacg accttgcccg
     1801 tgtagatgtc cccggactcg acgtccttgg tcatcccgtt tatggccgag agcgcaccct
     1861 ttacagagac cccgtcaacc ccggcgacga agaccgtgcc gtcgttctcg acggagatgt
     1921 tgacgccgta ctcgtcctgc agggcgttta tcgtcttgcc gccgggaccg atgaggagcc
     1981 cgatcttgtc cgtcggtatc tgcaacacct cgacgcgcgg ggcgtagtcg gagacctcgg
     2041 aacgcggctc ggcgatggtc tcgcgcatga tgtccaggat ctcgatcctg cccctcttgg
     2101 ccttctcgag cgcttccttg aggagctgcg cggagacgcc ggtgatcttc atgtccatct
     2161 ggagcgccgt tatgccgtcg cgcgtgccgg cgaccttgaa gtccatgtcg cccatgtggt
     2221 cctcgagccc ctggatgtcg gtgaggatga cgtagtcgtc gccctccttc accaacccca
     2281 tcgcgacccc cgagacaggg gcctttatcg gcacgccgcc gtccatcagg gcgagggtcg
     2341 agccgcacac gctcgccatt gaggaggacc cattggactc gagaacgtcg gagattatcc
     2401 gcagagcgta aggaaaggac tcctcgtccg ggatcaccgg caggagcgcg cgttcggcga
     2461 gcgccccgtg cccgatctcg cgccgccgag ggctgccgag cctccccgtc tcgccggtgg
     2521 agaacggcgg aaagttgtag tggtgcatgt agcgcttgct gg
//
LOCUS       DASTUV010000550         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1771728, whole genome shotgun sequence.
ACCESSION   DASTUV010000550 DASTUV010000000
VERSION     DASTUV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771728"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFT03_11120"
     CDS             <1..692
                     /locus_tag="VFT03_11120"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEU4848741.1"
                     /translation="PLRARVRSEGWLGRSTVRWTLTLYRDEPRLHLRVEINFSERFKL
                     LQMPVHLAAPPNRRTDGLPGGHVQRTPGPTEWPVQGWSRVDVADRQLALVTGDAYSLS
                     LDDTRWQWTLLRSPKMAWGGGEPGVYAGRDRHTDQGSHTFDFVLIVGDHLEETGLHTA
                     ARQQMQPPVVFDRYEGMDRPPWGNTPPRALWLGAVERALADGRMTHVLENEDVDSVRP
                     LFDKPERNREE"
     gene            696..1490
                     /locus_tag="VFT03_11125"
     CDS             696..1490
                     /locus_tag="VFT03_11125"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU4848742.1"
                     /translation="MAEFSGKVAIVSGGARGIGRAAARKLAGEGASVVICSDREEQVE
                     VTVAALREEGLEVRGVRADVTSSADMKKLMDLASETYGGVDVLVNSAGVQRYGTVVET
                     EEEVWDEVLDANLKGVYLASRYAIPEMRKRGGGAIVNLSSVQAFASQTGVAAYTASKG
                     GINALTRAMALDHAGENIRVNAVCPASVDTPMLRWSADLFKGDKSVEETLEDWGRMHP
                     LGRVARPEEVAEVIAFLASARASFITGGDYKVDGGMLAALGVRLPD"
     gene            1565..>4204
                     /locus_tag="VFT03_11130"
     CDS             1565..>4204
                     /locus_tag="VFT03_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEU4848743.1"
                     /translation="METYDRHRLERHARRIEELRLWRNAYESTVKGWRFSAGEEAGAS
                     GKTHDIEPGGFWPEIGIPVNLSARTNVPEEWAGLPVELELWLGGEGFVEIFVGDGCTA
                     SGLNPFHRSFPVLDRARGGEEVGIEAEVVSKGPFGSNFSEPRLERAQLVVPETETRAL
                     ERDLTSIFEACAALDDHEAVPHLLDVLDAAAAVLSDAWPTATGVTLTRYLEGYVNPIG
                     NAAQSLPPHYAEKALDINRMLGEPWSLPPAPETLGPLPGEAREAVRKARRVVAECLGR
                     IKEEYPPVGRLALTGHAHLDLAWLWPLEETRRKARRTFASVLGLMDRYEDFTFNQSSA
                     QLYEWVEQDAPELFERVKERVAEGRWETVGGSWVEPDCQIPSGESFTRQLFYGQRYFE
                     EKFGRRSSVAWFPDTFGYSPGLPQLLRGAGLSGFFTYKLNWSETNRFPHDLFVWEGID
                     GSRVVAHTFENPGTDYNGDITPIDLYGTWRNFKGKRNFRESLFSFGWGDGGGGPSEKM
                     LENYDRLKCFPAMPRLRMARVDGFFASLPQDGLPRWAGELYLELHRGTLTTQARVKKL
                     NRQAEHRLLEAEAFATMAALSGRPYPAEELERLWKVLLLNQFHDVLPGTSISEVYQDA
                     HRQLEETVSGAERLRDGALHTLARGTAGAQRSVTVANAALHPRPLTVLLPAEPGVTPA
                     DAKGRPLPAQETASGLLVHAPDREVPGLGWVSLALRDETQDASSAGSASGVEAGEAGG
                     GAFIENGLLRVEISAGGSLGRVYDKEAEREVLDGAGNQLWTYADKPPNWDAWDINEDY
                     EMEGEEVPGASSVEVVEAGPLRGAVRVERRFRGSSISQTYRLLSASRRLDLDARVDWR
                     ERQVLLRALFPLNVRSHEATFETM"
BASE COUNT          728 a         1355 c         1487 g          634 t
ORIGIN      
        1 ggcccttgcg ggcgcgggtc cgctcggaag gatggctggg ccgctccacc gtgcgatgga
       61 cgctcaccct gtatcgtgac gagccccggc tccacctcag ggtggagatc aacttcagcg
      121 agcgcttcaa actccttcag atgccagtcc atctggcggc tcctcccaat cgccggaccg
      181 acgggctgcc cggcggtcat gtgcagcgca ctcctggtcc gacggagtgg cccgtccagg
      241 gctggtcgcg cgtggacgta gcggatcgtc aactggccct ggtaaccggc gacgcctaca
      301 gcctgagcct ggacgatact cgctggcagt ggaccctgct caggagcccg aagatggcct
      361 ggggaggggg cgagccaggg gtgtacgccg ggcgtgaccg gcacacggac caggggtcgc
      421 acaccttcga cttcgtcctc atagttgggg atcatctgga agagaccggg ctgcacaccg
      481 cggcccggca gcagatgcag cctcccgtcg tgttcgaccg ctacgagggc atggaccgcc
      541 cgccctgggg aaacactccc ccgcgagctc tctggcttgg cgcggtggag cgtgccctgg
      601 cagacggaag gatgacccac gtgctggaga acgaagacgt ggacagcgtg cgtcccctct
      661 tcgacaagcc cgaacggaat cgggaagagt agagcatggc ggagttctca gggaaggtag
      721 cgatcgtctc cggcggcgcg cggggtatcg gccgggctgc ggcccggaag ctggcgggag
      781 aaggcgcctc ggtcgttatc tgcagcgacc gcgaggagca ggtagaagtg acagtcgccg
      841 cgttgcgcga ggagggcctc gaggttcgcg gggtgagggc ggacgtgact tcgtcggccg
      901 acatgaagaa gctgatggat ctcgcaagcg agacctatgg tggcgtggac gtcctggtga
      961 acagcgccgg tgtgcagcgt tacgggaccg tagtcgagac cgaagaagaa gtgtgggacg
     1021 aggtcctcga cgcaaacctg aagggggtct acctcgcctc gagatacgcc ataccggaga
     1081 tgaggaagcg tggtggagga gcgatagtca acctctcctc cgtgcaggcc ttcgcctctc
     1141 agactggagt ggcggcctac acggccagca aagggggcat caatgctctc acccgcgcga
     1201 tggccctgga tcacgcgggt gagaatatcc gggtgaacgc ggtctgcccg gcctcggtgg
     1261 acacgccgat gctgcggtgg tcggcggatc tgttcaaggg ggacaagagc gtcgaggaga
     1321 ccctggagga ctggggcagg atgcacccgc tgggaagggt ggcaagacca gaagaggtcg
     1381 cagaggtcat cgccttcctc gccagcgcca gggcgagctt catcaccggt ggggattaca
     1441 aggtcgacgg cggcatgcta gccgcgctcg gcgtccgcct gcccgactag gcgacgctgg
     1501 agtgtgccac cgcgtcgata acctgtagta tctcggacgt gtccgtatgc caagaaggag
     1561 accgatggag acctacgacc gtcaccggct ggagcgccac gcccgccgga tcgaggagct
     1621 gaggctctgg cgcaacgcct atgagagtac tgtgaagggc tggcgcttct cagccgggga
     1681 agaagcaggg gcttccggga agacccacga catcgagccc ggcggcttct ggccggagat
     1741 cggcatcccg gtcaacctct cggcccgcac gaacgtccct gaggagtggg cggggctacc
     1801 cgtggagctg gagctctggc tcggtggcga ggggttcgtc gagatatttg tcggcgacgg
     1861 ctgcacagcg agcggcctca accccttcca ccgcagcttt cccgtgctgg acagggcgcg
     1921 aggcggggag gaggtaggca tcgaggccga ggtggtctcg aagggcccgt tcggctcgaa
     1981 cttctcggag ccgcgcctcg agcgggcgca gctcgtggtg cccgagacgg agacgcgggc
     2041 gctggagcgg gacctcacgt caatcttcga ggcgtgtgcc gcgctcgacg accacgaggc
     2101 cgtgccgcat ctgctcgacg tactcgacgc cgcggccgcc gtgctctctg acgcctggcc
     2161 gaccgcgacc ggcgtcaccc tcacccgcta cctcgagggg tacgtcaacc ccataggcaa
     2221 cgcggcgcag agcttgccgc cgcactacgc cgagaaggcc ctcgacatca accggatgct
     2281 cggcgagccc tggagcctgc cgccggctcc cgagaccctc ggccccctcc ctggcgaggc
     2341 acgggaggcc gttcgcaaag cgcgccgggt agtcgctgag tgtctcggtc gcatcaaaga
     2401 ggagtacccg cccgtcgggc ggctcgcgct cacaggccac gcgcacctgg acctcgcgtg
     2461 gctctggccc ctcgaagaga cgcggcgcaa ggcgaggagg accttcgcga gcgtcctcgg
     2521 gctcatggac cgctacgaag actttacctt caaccagtcc tcggcccagc tctacgagtg
     2581 ggtcgagcag gacgcgcccg agctcttcga gcgggtgaaa gagcgcgtcg cggaagggag
     2641 gtgggagaca gttggcggct cctgggtaga gccggactgc cagatcccct ccggcgagtc
     2701 tttcacgcgg cagctcttct acggccagcg ttacttcgag gagaagttcg gacgccgctc
     2761 gagtgtggcc tggttccccg acaccttcgg ttactcgccc ggcctccccc agctcctgcg
     2821 cggcgcggga ctatccggct tcttcacgta caagctcaac tggagcgaga ccaaccgctt
     2881 cccccacgac ctcttcgtgt gggagggcat agacggcagc cgcgtcgtcg ctcatacctt
     2941 cgagaacccc ggcacggact ataacggcga catcacccct atcgacctgt acggcacgtg
     3001 gcgcaacttc aagggcaagc gcaacttcag ggagagcctg ttctcgttcg gctggggcga
     3061 cggcggcggc ggtccgagcg agaagatgct cgagaactac gaccggctca agtgcttccc
     3121 ggcgatgccc cgtctgagaa tggcccgcgt ggacgggttc ttcgcctcgc tcccgcagga
     3181 cggcctcccc agatgggccg gcgagctcta cctcgagctc caccgcggca ccctgaccac
     3241 ccaggcgagg gtcaagaagc tcaaccgcca ggccgagcac cggctcctcg aggcggaagc
     3301 cttcgccacg atggcggctt tgagcggcag gccatacccg gcggaggagc tcgagcggct
     3361 gtggaaggtg ctcctcttga accagttcca cgacgtcctg ccgggaacct cgatctccga
     3421 agtctaccag gacgcccacc gccagctcga agagacggtg agcggcgccg agaggttgcg
     3481 tgatggagcc ctgcacaccc tggcgcgggg gacggctggc gcgcagagaa gcgtaaccgt
     3541 ggccaacgcc gccctgcacc cgagacccct cacggtgctc ctgccggcgg agccgggggt
     3601 aacgccagcc gacgcgaagg gcagacccct gccggctcag gagaccgcca gcggtctcct
     3661 cgtccacgcg ccggaccgcg aggtgcccgg tctgggatgg gtctcgctgg cgctgcgcga
     3721 cgagacgcag gacgcctcct cggcagggag cgcatccggt gtcgaagccg gggaggccgg
     3781 gggaggggcg ttcatcgaga acgggttgct gcgggtggag atctccgccg gcggcagcct
     3841 cgggcgcgtc tacgacaaag aggcggagcg cgaggtgctc gatggagccg ggaatcagct
     3901 ctggacgtac gcggacaaac cgcccaactg ggacgcctgg gacattaacg aggactacga
     3961 gatggaaggg gaggaggtcc ctggagcctc cagtgtggag gttgtggaag ccgggcccct
     4021 gcgcggcgcg gtccgcgtcg agcggcgttt tcgggggtcc agtatctccc agacctacag
     4081 gctgctttcg gcctcgaggc gcctggacct cgacgcgcgc gtggactggc gcgagcggca
     4141 ggtcctgctg cgggcgctct tcccactgaa cgtacgctcc cacgaggcga ccttcgagac
     4201 tatg
//
LOCUS       DASTUV010000551         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_412248, whole genome shotgun sequence.
ACCESSION   DASTUV010000551 DASTUV010000000
VERSION     DASTUV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412248"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFT03_11135"
     CDS             <1..207
                     /locus_tag="VFT03_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848744.1"
                     /translation="REQGNTPSLPNQAGFMQNAGYEFRRIPTNAKFRKEYRTGSLARI
                     EVFGLLHAVLECKAGEGFFCRVAA"
     gene            complement(674..976)
                     /locus_tag="VFT03_11140"
     CDS             complement(674..976)
                     /locus_tag="VFT03_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU4848745.1"
                     /translation="MVTEERVRDQLRNVIDPEIGMDLVELGLIYDVGVHDEGRHIDVT
                     FSLTSPMCPVGDLIQEQVETETLSIEGVETVNAQLTFEPMWNPEMMSPAAKLFFGR"
     gene            complement(1182..2711)
                     /locus_tag="VFT03_11145"
     CDS             complement(1182..2711)
                     /locus_tag="VFT03_11145"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEU4848746.1"
                     /translation="MDERTRLGLGVLGAALFLGVLGDGLLRATPWGINLLVWVAALAG
                     ATVAVAWRGRLRVAGEGRWLVPVALVFAAGLAWRDSPTVVLLNLLAFLVAVSLAALWG
                     RRGRLRLAGVSEYVLGGIYAGTLSSAGPVPVAASEIGWQQVARGRWRSPALAAARGVF
                     FAAPLLLVFGALFVAADAVFEQLVVDLFGFDVAEVFGHISLMLVFAWFTAGLLWVALL
                     AHTRENLALRRPDALSLGIVELGIVLGLLDLLFLAFVAVQVRYLFGGAERVVTTAGLT
                     YAEYARRGFFELVTVTALALPILLLTHWLLRAEGRGSRRVFHLLAGTLIALLFVVMAS
                     ALQRMYLYTQVYGLTELRFYATIFMAWLAVVLVWFLLTVLRGRRDRFAFGALLSGFAA
                     VLLINAMNPDALIARANLQRLEEGKRFDAYYLTTLSADAAPVLFDALPELSGKRIAPE
                     ANLTMEQVILDRYREDEQGDWRTWNLSRSRAGYLAESYPSSGGIAAKDRAAQAPEVNS
                     R"
     gene            complement(2863..>3146)
                     /locus_tag="VFT03_11150"
     CDS             complement(2863..>3146)
                     /locus_tag="VFT03_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4848747.1"
                     /translation="LVPNVWLLVVPAVLFGVAQGINLPNVFSLLNAHAPSENRGAFMA
                     TNGMSLRAGQTIGPLIMAATAGTLGLTGAYLAATALAVVALLLVITLLR"
BASE COUNT          625 a          989 c         1010 g          522 t
ORIGIN      
        1 agggagcagg gaaacacgcc ctcgctgccc aatcaggccg ggtttatgca gaatgctgga
       61 tatgaatttc ggagaattcc aaccaacgcg aagttcagaa aggagtatcg gaccggttcg
      121 ctagcgagaa tcgaggtctt cggtctgctg catgcagtgt tggaatgcaa ggcgggcgaa
      181 ggcttcttct gtagggttgc cgcgtagcct tgccctcttt tcttcggcga tccagaaagc
      241 cgcttctatc cacgcttccc gttgcggcgt ccacgggaag aggtcttgag attcctcgta
      301 gaaaccttga gatcccatca ctttatttta gcccaaggta catggccgcg gtcctaatgg
      361 gccaggaccg ccgggaagct ggtaggtagt cgcgcccagg ccttagctgg ctcctccgcg
      421 gcgcttgtgg gcgattcttg tggctctcag cgcacttcta cgccccgctc agataaggtg
      481 atgtcaaccc tgctggccga cgatggtgcg aaccagggag ccgtaggcgt cgtcggggcg
      541 gccgcggcgg cgtgcctcgt ggtcgagcgg gccgatgcgc tcaaccagcc cggccatgac
      601 cggatcgccg caatcgcgaa tgaagtgaga gcccgcgtcc gggtgcgcgg gctcctccac
      661 tctggaagtc aacctaccgg ccgaagaata gcttggcggc cgggctcatc atctccgggt
      721 tccacatcgg ctcgaaggta agctgggcgt tcaccgtctc gacgccctcg atactaaggg
      781 tctcggtctc tacctgctcc tggatcagat cccccaccgg gcacatcggg ctcgtcaggg
      841 agaatgtgac gtcgatgtgg cgtccctcgt cgtgaacgcc gacgtcatag atcaaaccga
      901 gctccacgag gtccatcccg atctccgggt ctatcacgtt tctaagctgg tccctcactc
      961 gctcctcggt taccacgctc tcacctcttt ttggcttgct ctagttttct aattgtatct
     1021 gcacccgggt ccgttgccac caaagccacc tgcgctcccg cgagcttgcg gttttgttaa
     1081 cctcttgtta cacctgatgc acttatcccc cgctctggcg ctcttttcca caacatgttg
     1141 tggataactt tcgaaacgcg gtgcccaact accctatatt gttacctgga gttaacttct
     1201 ggagcttgcg ccgctcggtc cttggccgcg attcctccgg aagaggggta ggactcggcc
     1261 aggtatccgg ctcgtgaccg gctcaggttc caggttcgcc agtcaccctg ctcgtcttcg
     1321 cggtagcgat ccaatatcac ctgctccatc gtcaggttgg cttcgggggc gatgcgtttc
     1381 ccggataact ccgggagcgc gtcgaatagg acgggagcgg cgtcggcgct cagggtggtg
     1441 aggtagtagg cgtcgaacct cttcccctcc tccaggcgct ggaggttcgc tcgggctata
     1501 agggcgtcgg ggttcatggc atttatgaga agaacggccg cgaagccgga gaggagggcg
     1561 ccgaacgcga agcggtcgcg ccgaccccgc agtacggtca gcaggaacca gaccagcacg
     1621 acggcgagcc aggccatgaa gatggtggcg tagaaacgca gctcggtcag cccgtacacc
     1681 tgagtataga ggtacatccg ctgcaaggcc gaggccatga cgacgaagag aagggctatg
     1741 agggtccccg cgagaaggtg gaagacgcgc cggctgccgc ggccctcggc gcgaaggagc
     1801 cagtgggtca ggagcaggat cggcagcgct agcgccgtca cggtcacgag ctcgaagaag
     1861 ccgcgcctgg cgtactcggc gtaggttaga ccggctgtgg ttacgaccct ctcggcgcct
     1921 ccgaagaggt aacgcacctg gacggccacg aaggcgagga aaagcaagtc gagcaggccg
     1981 aggacgatgc cgagctcgac gatgccaagc gagagggcgt cggggcgcct cagggcaagg
     2041 ttctcgcgcg tgtgggccag caaggctacc cacagcagtc ctgcagtgaa ccaggcgaaa
     2101 acgagcatca gggagatgtg gccgaagacc tcggcaacgt cgaagccgaa gaggtctacg
     2161 accagctgct cgaagacagc gtcggcggcg acgaacagcg cgccgaagac aagcaggagc
     2221 ggggctgcga agaacacgcc ccgagccgcg gcgagcgcgg gcgaccgcca cctgccgcgc
     2281 gcgacctgct gccacccgat ctcgcttgct gccacgggga cagggccggc ggagctgagg
     2341 gttccggcgt agataccgcc gagcacatac tccgagacgc ctgctagccg cagtcgaccc
     2401 ctccgccccc acaacgcggc gagcgagacc gcaacgagga aagccaggag gttcaacaat
     2461 acgaccgtcg gggaatcccg ccacgcgagg ccggccgcga acacgagagc cacaggtacg
     2521 agccagcgcc cctcacccgc gacccgcagc cgcccgcgcc aggcaacggc aaccgtggcg
     2581 ccggcgagcg ccgcgaccca cacgagaagg ttgatccccc aaggtgtcgc ccgcagcagc
     2641 ccatcgccaa gcaccccgag aaacagcgcc gccccgagca cgccaagccc cagcctcgtc
     2701 ctctcgtcca tcccacacct ccgctagcca cccttgcggg gctgcactct agcggacgga
     2761 tataacagaa cgggcgacgg tccggttaaa aaggtcgaag cttgttgaag gcctgcgtcc
     2821 ccgagataac ataccgtaat ccatcgccaa cgataccgcg cttcagcgga ggagggtgat
     2881 gaccagcagg agggcgacga cggcgagggc agtggccgcg aggtaggcgc cggtgagacc
     2941 aagggtgcct gcggtagccg ccatgatcag gggtccgatg gtctgtccgg cgcgcagcga
     3001 catcccgtta gttgccatga aggcgccacg gttctccgag ggggcgtgag cgttcagaag
     3061 ggagaagacg ttgggaaggt tgatcccctg cgccacgccg aagaggacgg cgggaacgac
     3121 gagcagccac acattcggga cgagag
//
LOCUS       DASTUV010000552         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_818902, whole genome shotgun sequence.
ACCESSION   DASTUV010000552 DASTUV010000000
VERSION     DASTUV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818902"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /gene="trxB"
                     /locus_tag="VFT03_11155"
     CDS             <1..934
                     /gene="trxB"
                     /locus_tag="VFT03_11155"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU4848748.1"
                     /translation="YAARANLDVLVFQGFEVGGQLMLTSDVENYPGYRDGVMGPEMMD
                     DFEAQAARFGAQMRHENVERVDFSERPFKLWAEGEEEPTLARSVIIATGAKAKWLGLP
                     GESRLMGRGVSGCATCDGFFFKDKRVAVVGGGDTAMEEALFLTKFADEVLLIHRRDEF
                     RASKIMLERARKNPKVTFLTDTVIQDVLGENSVEGIRTKNLKTGEEREIEVEGFFAAI
                     GHAPATGLFKDQVEMDEGGYILQKGHTMTSVPGVFAAGDVSDKRYRQAVTAAGDGSRA
                     AIDAERWLEEQGEAEGAEDPAVWLAAKDKDAASL"
     gene            complement(962..2668)
                     /gene="polX"
                     /locus_tag="VFT03_11160"
     CDS             complement(962..2668)
                     /gene="polX"
                     /locus_tag="VFT03_11160"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU4848749.1"
                     /translation="MRNGEIVRALEDIATLMQIKGEEYYRYLGYQRAAESVGALGRPV
                     TEVEDLTQLPHVGPTTAEVIRDLAEDRTPQILADLLAEIPTGLVEMTHLPGVGPRTVH
                     TLWKELGVVSVEELAGLEEDQISSLKGFGKKSAEKILRAARTYNAQERRMLLNTATAL
                     GEEMLEFVRSHPSTIEAEVAGSLRRKKETIGDLDVVAASTDQGSLADAFADAPFADEV
                     LAHGPTKVYIVRDGVEIDLRIVEPEAFGSLLHHFTGGQAHNIALRERAVKMGINISEY
                     GLAEEGTGNYEPVATEEELYGRLGLPYIPPELREDTGEIEAAERGELPDLIEVSDLRG
                     DLHVHTNWSDGKGTIESMAEAAIERGYEYLVFSDHSQSLRVANGLSPERLKAKIEAVR
                     RADEEYGEIHLLNGSEVDILRDGSLDYEDALLAELDFVVASVHTSFNVGEEAMTERII
                     RAMNNPYVRTIGHPTGRILNRREPYEVDVSRLIREAAATNTALEHNAYVDRLDLSVPY
                     MREAINAGVRITIDTDAHDETALSFAKFGISQARRAWVEKENVINTLPWNEFESYLKS
                     GK"
     gene            2791..>3458
                     /locus_tag="VFT03_11165"
     CDS             2791..>3458
                     /locus_tag="VFT03_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU4848750.1"
                     /translation="MPGSDIEAVVFDLDGVIVDSEHVWDAAREALARERGGRWHEGAQ
                     QDMMGMSSVEWSRYMHDVIGLKDPPEEISAEVARRLEATYREELPLIDGATEAVARLA
                     ERWPLAVASSSNRPIIDLVLGLSGLDRFFRATVSSEEVPRGKPAPDVYLEAARRLGAD
                     PERSAAVEDSRSGILSARAAGMRVVAIPNMRFPPGEDALAAADVVIPSIGELTPDVVE
                     GSELP"
BASE COUNT          533 a         1145 c         1164 g          616 t
ORIGIN      
        1 gtacgccgct cgggcgaacc tcgacgtgct ggtgttccag ggtttcgagg tcggcgggca
       61 gctcatgctc acctctgacg tggagaacta ccctggctac agggacggcg taatgggtcc
      121 tgagatgatg gacgacttcg aggcccaggc ggcccgcttc ggggcacaga tgcgccacga
      181 gaacgtagag cgggtagact tctccgagcg cccgttcaag ctctgggccg agggcgagga
      241 ggagcctact ctagccaggt ccgttattat cgctacgggg gccaaggcca agtggctcgg
      301 gctccccggg gagagccgcc tcatgggccg cggggttagc ggctgcgcca cctgcgacgg
      361 cttcttcttc aaggacaagc gcgtcgcggt cgtcgggggc ggggacacgg ccatggagga
      421 ggcgctcttc ctcaccaagt tcgccgacga ggtgctcctg atccaccgcc gcgacgagtt
      481 ccgggcgtcc aagatcatgc tcgagagggc gcgcaagaac cccaaggtca ccttcctcac
      541 cgacactgtg atccaggacg tccttggcga gaactcggtc gaggggataa ggacgaagaa
      601 cctgaagacg ggagaggagc gcgagatcga ggtcgagggc ttcttcgcgg ccatcggcca
      661 cgccccggcg accggcctct tcaaggacca ggtcgagatg gacgagggcg gctacattct
      721 ccagaagggg cacaccatga cgagcgtgcc tggcgtcttc gccgcaggcg acgtctcgga
      781 caagcgatac cgccaggccg tcactgccgc cggcgacggc tcccgcgcag ccatcgacgc
      841 cgagcgctgg ctcgaggagc agggcgaggc cgaaggggca gaagaccctg ccgtctggtt
      901 ggccgcgaag gacaaggacg cggcgagcct gtaagcccgc gcgtaaccca cgcctccggc
      961 gttacttacc gctcttcagg tagctctcga actcgttcca ggggagagtg tttatgacgt
     1021 tctccttctc tacccacgcg cggcgggcct gggagatgcc gaacttcgcg aagctcagag
     1081 cggtctcgtc gtgggcgtcg gtgtctatgg tgatacggac gcctgcgttt atggcttccc
     1141 tcatgtaggg gacggagagg tcgaggcggt cgacgtaggc gttgtgctcc agggccgtgt
     1201 tcgtagcggc tgcctctctt atgaggcgcg agacgtccac ttcgtatggc tcgcggcggt
     1261 tcaggatgcg gcctgtcggg tggccgatgg tgcggacgta agggttgttc atcgcccgga
     1321 tgatgcgctc cgtcatcgcc tcctcaccga cgttgaacga ggtgtggacc gaggcgacga
     1381 cgaagtccag ctcggcgagc agggcgtcct cgtagtcgag ggagccgtcc ctgagtatgt
     1441 ccacctctga gccgttgagg aggtgtatct cgccgtactc ctcgtctgct ctcctcaccg
     1501 cctcgatctt cgccttcagg cgctccgggg agaggccgtt cgccaccctc aggctctgcg
     1561 agtggtcgga gaagaccagg tactcgtagc cgcgctcgat cgcggcctcc gccatgctct
     1621 ctatggtccc tttgccgtcc gaccagttcg tgtgcacgtg caggtcgccg cgcaggtcgg
     1681 agacctcgat tagatcgggc agctcgcctc tctcggcggc ctcgatctcc cccgtgtcct
     1741 cccttagctc cggcgggatg tacggcagcc cgagccgccc gtagagctcc tcctcggtag
     1801 ctacgggctc gtaattgccc gtcccttcct ccgccagacc gtactccgag atgtttatgc
     1861 ccatcttcac ggcccgctcg cgcagggcga tgttgtgggc ctggccgccg gtgaagtggt
     1921 gcaggagcga gccgaacgcc tcgggctcga ctatccggag gtctatctcg accccgtcgc
     1981 gcacgatata gactttcgtc ggcccgtggg cgaggacctc gtctgcgaac ggggcgtcgg
     2041 cgaaggcgtc ggcgagcgac ccctggtccg tgctcgctgc cacgacgtcc aggtcgccta
     2101 tggtctcctt cttgcgcctg agggagccag cgacctcggc ttcgatggtg gaaggatgtg
     2161 agcgaacgaa ctcgagcatc tcctctccca gagcggttgc cgtgttgagc agcatgcggc
     2221 gctcctgggc gttgtaggtg cgggcggcgc gcaggatctt ctcagcgctc ttcttcccga
     2281 agcccttgag ggaggagatc tggtcctcct caagcccggc gagctcctcg acgctcacga
     2341 cgccgagctc cttccagagg gtgtggaccg tacgcggccc caccccgggt aggtgcgtca
     2401 tctcgaccag gccggtcggt atctccgcga gcaggtcggc gaggatctgg ggcgtgcggt
     2461 cctcggcgag gtcccggatc acctccgccg tcgtcgggcc tacgtggggc agctgcgtga
     2521 ggtcctcgac ctccgttacc gggcggccca gagcgcccac ggactcggcg gcccgctggt
     2581 agccaaggta gcggtagtac tcctcgccct tgatctgcat gagggtcgct atatcctcga
     2641 gggcgcggac tatctctccg tttcgcaact tgcctcctag ctcgtaggac gaaatcggtc
     2701 ccagcactag tttaccgctc cgcgccgcag ttccatccca ggataatccg cgccggccct
     2761 tcactcgtgt cccgtgtaga atcgggcacc atgccgggct cggacataga ggcggtcgtc
     2821 ttcgacctcg acggggtgat cgtggactcc gagcacgtct gggacgccgc ccgcgaggct
     2881 ctggcgcgcg agcgcggcgg acgctggcac gagggcgcgc agcaggacat gatggggatg
     2941 agctccgtcg agtggtcgcg ctacatgcac gacgtgatcg gcctgaagga ccctcccgag
     3001 gagatctccg ccgaggtcgc caggcgcctc gaggctacct accgcgagga gctgccgctg
     3061 atagacggag ctacagaagc tgtcgcccgc ctcgccgaac gctggccgct ggcggtcgcc
     3121 tcctcgtcga accgccccat aatcgacctg gtcctcgggc tctcaggact tgaccggttc
     3181 ttccgggcga cggtctcctc cgaggaggtg ccgcgcggca agcccgcccc tgacgtctac
     3241 ctcgaggccg cccggcgcct cggcgccgac ccggagagga gtgccgccgt ggaagactcc
     3301 cgcagcggca tcctctcggc cagagccgcg ggcatgcgcg tcgtggcgat ccccaacatg
     3361 cgcttcccgc ccggcgagga tgcactcgcc gcggcggacg tcgtcatccc ctccatcggc
     3421 gagctcacgc cggacgtcgt ggaagggagc gagctccc
//
LOCUS       DASTUV010000553         4277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_877545, whole genome shotgun sequence.
ACCESSION   DASTUV010000553 DASTUV010000000
VERSION     DASTUV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877545"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..593
                     /locus_tag="VFT03_11170"
     CDS             255..593
                     /locus_tag="VFT03_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEU4848751.1"
                     /translation="MVELAWEDFERVDVRVGRVVEAKPFPEARKPSIKLSIDFGPEVG
                     EKRTSAQLTEHYTPDSLVGRQVVAVVNFPPKRIAGFKSEVLVLGVPDEAGEVVLLSPD
                     LEVPPGGRMF"
     gene            complement(590..817)
                     /locus_tag="VFT03_11175"
     CDS             complement(590..817)
                     /locus_tag="VFT03_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848752.1"
                     /translation="MFGEGNKANASLPRYEKGDRVFVRGTRAPRLPGVVIGHYVDRNL
                     YRVVYMVDVAGESPRALEEWRLSPRREGEER"
     gene            951..1622
                     /locus_tag="VFT03_11180"
     CDS             951..1622
                     /locus_tag="VFT03_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEU4848753.1"
                     /translation="MSETNLEQFREVAPAGKFTLQNSAMLKVVLQGDQIQARVGSMVA
                     YQGELRFEYQSGGLGRFFKKAMTGEGVKLMTATGTGDLFLAHDKRKIMILDLENERMT
                     VNGDNVLAIEPGIEWDIHRVEGAGRLSGGLFNVVLHGTGKVAVTSDGEPVLLDTSRPT
                     FADPESAIAWSGNVRTGIKSDVSFKTFTGRGSGESFQMSFEGPGWVLIQPSEGSVVPE
                     HSHGG"
     gene            complement(1677..4112)
                     /gene="leuS"
                     /locus_tag="VFT03_11185"
     CDS             complement(1677..4112)
                     /gene="leuS"
                     /locus_tag="VFT03_11185"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU4848754.1"
                     /translation="MSEASTTKRDYDPITLERRWQERWAESGLYRTDEDPEKTKHYAL
                     TMLPYPSGDLHVGHWYAMTPSDTRARFMRMRGYKVFFPIGFDAFGLPAENAAINRGIH
                     PYRWTISNIENMRGQIRQMGTMFDFDAEVVTCDPEYYRWNQWFFLKFFEKGLAYRKEV
                     PVDWCPKDNTTLAREQVVGPDRRCERCGTPVIRKNLVQWLFKITDYAEELLDFSNIEW
                     PERVRALQTNWIGRSEGAEIFFEVERYGTIEVFTTRPDTVFGATFFVLAPEHPAVEQI
                     TTPERSDEVRAYVEKASRMTEIDRTDVTREKTGVFTGAYAINPATGEKIPVFVADYVL
                     MGYGTGAIMAVPGQDQRDWDFARKYDLPIIRTVRPPEDFDGEAYIGEGAAINSGFLDG
                     LNIEDAKARIIEWLEAQGKGRAAVTYRLRDWLISRQRYWGTPIPIIYCDNCGTVPVPE
                     EDLPVLLPEDAEFMPTGESPLKRDPDFYNTVCPTCGGPATRETDTMDTFVDSSWYWYR
                     YLSPHYGEGPWEPEKGEEWLPVDIYTGGIEHAILHLLYARFWTKTMRDLGLVEFGEPF
                     NRLYNQGIILGEDAEKMSKSRGNVVNPQDYLDRYGADALRDFLMFIGPWDQGGPWDAR
                     GIEGVSRFLRRALSLTGDGDPSGAEADPGELARRTNRTVKKVTEDLEGFRFNTAIAAL
                     MEQTNYLLAIKGQVGEEEWGDALRTFALVLAPFAPHHAEEMWAAMGEPYSVHDQAWPA
                     WDETLIAAEEVTLVVQVNGKLRDRIEVPADITEEAATELALSSERVRLHVEGREIRKS
                     IYVPGRLVNLVVG"
BASE COUNT          729 a         1383 c         1333 g          832 t
ORIGIN      
        1 cggtgccgat ttgccgaatt cgtcatctgg ctgcccgctc gagtgcgagc tcgcgtcttt
       61 tcctccaacg tctccctatt cttcgtgact ccaccaggtc ccctgggatc gactctttac
      121 gcgtctcgat tcacgttaca ggagaaacta ctcaggcaca accgcgctcg gatgccaagg
      181 cttgcctacc ttcatcttgt ggcccactat acgttctcca atcccttacc aaggacttga
      241 aaggagcttc cattatggtc gagctagcgt gggaagactt cgagagggtg gacgtgcggg
      301 tgggaagggt cgtcgaagcg aagccgtttc cggaggcgcg gaagccctcg ataaagctct
      361 cgatcgactt cggacccgag gtcggtgaga agaggaccag cgcccagtta acggagcact
      421 acaccccgga ctcgctcgtc ggcaggcagg tggtcgcggt ggtcaacttc ccgcccaagc
      481 ggatcgcggg cttcaagagc gaggtgctcg tgctcggggt gcctgacgaa gccggcgagg
      541 tggtgctcct ctctcccgac ctcgaagttc cgcccggcgg caggatgttc tagcgctcct
      601 ccccttccct gcgcggggag agccgccact cctcgagggc cctggggctc tcgccggcga
      661 cgtccaccat gtacacgacc ctgtacaggt tccggtccac gtagtggccg atgacgacgc
      721 cgggcagacg cggggctcgc gtgccccgga cgaacaccct gtcgcccttc tcgtaccggg
      781 gtagcgacgc gttcgccttg ttaccctcgc cgaacatacc ctgcctcgtt gcggcttccg
      841 aacatagcct acacgcggag ggaagcgggt gcatcacctc ttgtggaaac ctggactacg
      901 cgggtaacgt agtagatcat gctggtcgaa caagttcggg aggtgtggtg gtgagcgaga
      961 ccaatctaga gcagttcagg gaagttgcgc cggctgggaa gttcacgcta cagaactcgg
     1021 cgatgctcaa ggtcgtactg caaggggacc agatccaggc cagggtcggc tcgatggtcg
     1081 cctaccaggg cgagttgcgc ttcgagtacc agagcggggg cctggggcgc ttcttcaaga
     1141 aggccatgac aggcgagggc gtcaagctga tgacggctac ggggacagga gacctgttcc
     1201 tggcgcacga caagcgcaag atcatgatcc tcgacctcga gaacgagcgg atgaccgtca
     1261 acggcgacaa cgtgctggct atcgagccgg gcatcgagtg ggacatccac agggtcgagg
     1321 gcgcgggcag gctctccggc ggcctcttca acgtcgtcct gcatgggacg ggcaaggtgg
     1381 ccgtcacctc cgacggggag ccggtcttgc tcgacacgag cagacccacc ttcgccgacc
     1441 ccgagtcggc tatcgcctgg agcggtaacg tccgtaccgg catcaagagc gacgtctcgt
     1501 tcaagacctt cacgggccgc ggctcagggg agtcgttcca gatgtccttc gaaggtcccg
     1561 gctgggtcct catacagccc tcggaaggct ctgtggtgcc cgagcactct cacggaggct
     1621 aaagtgtcgg aggagggctc gctcttcggc gattaggctt cagttataac tgaaacctac
     1681 cccaccacga ggttgacgag ccgccctggc acgtagatgc tcttcctgat ctcacgtccc
     1741 tcgacgtgga gccgcaccct ctcgctggag agcgccagct ctgtggctgc ctcctctgtg
     1801 atgtccgccg ggacctctat gcggtcgcgc agcttcccgt tgacctgcac gaccagcgtg
     1861 acctcctccg ccgctatcag ggtctcgtcc cacgcaggcc acgcttggtc gtgcaccgag
     1921 tagggctcgc ccatagcagc ccacatctcc tcggcgtggt gcggcgcgaa cggcgcgagg
     1981 acgagcgcga aggtcctcag ggcgtctccc cactcctcct cccccacctg gcccttgatc
     2041 gcgagcaggt agttggtttg ctccataagc gcggctatag ccgtgttgaa ccggaacccc
     2101 tcgaggtcct ccgtgacttt cttgacggtc ctgttggtcc gcctggcgag ctctcccggg
     2161 tcggcctcgg cccccgacgg gtcgccgtcg cccgtgagcg agagggctcg cctcaagaaa
     2221 cgtgagacgc cctcgatacc gcgcgcatcc cagggtccgc cctggtccca gggacctatg
     2281 aacatcagga agtcgcgcag ggcgtccgcg ccgtagcggt caaggtagtc ctgcgggttg
     2341 accacgttgc cgcggctctt gctcatcttc tccgcgtcct cgcccaggat gatgccctgg
     2401 ttgtagagcc ggttgaatgg ctcaccgaac tccacgagcc cgaggtctcg catcgtcttg
     2461 gtccagaaac gggcgtagag caggtgcagg atggcgtgct cgatgccgcc ggtgtaaatg
     2521 tctaccggca gccactcctc gcccttctcc ggctcccacg gcccttcgcc gtagtgcggc
     2581 gagaggtacc ggtaccagta ccatgaggag tccacgaaag tatccatagt gtccgtctcc
     2641 cgcgtggccg ggccgccaca ggtgggacac accgtgttgt agaagtccgg atcgcgcttg
     2701 agcggcgact cgccagtcgg catgaactcc gcgtcctcgg ggagcaggac gggcaggtcc
     2761 tcctcgggga ccgggaccgt gccgcagtta tcgcagtaga taatgggtat gggcgtgccc
     2821 cagtagcgct ggcgtgagat cagccagtcg cggaggcggt acgtaacggc ggcgcggccc
     2881 ttgccttgcg cttcgagcca ctcgataatg cgcgccttcg cgtcctcgat gttcaggcca
     2941 tcgaggaagc cgctgttgat cgccgcaccc tcgccgatgt aagcctcgcc gtcgaagtct
     3001 tctgggggcc gcacggtgcg gattatcggc agatcgtact tcctggcgaa gtcccagtcg
     3061 cgctggtcct gccccgggac cgccatgatc gcccccgtgc cgtagcccat gagcacgtag
     3121 tccgcgacga agaccgggat cttctcgccg gtcgccgggt ttatggcgta ggcgccggtg
     3181 aagacacccg tcttctcccg cgtcacgtct gtccggtcga tctcggtcat cctgctcgcc
     3241 ttctcgacgt aggcgcgcac ctcgtcggag cgctcgggcg tagttatctg ctcgaccgcc
     3301 gggtgctcgg gagcgagcac gaaaaacgtg gcgccgaaga cggtgtccgg acgtgtagtg
     3361 aagacttcga tagtcccgta gcgctccacc tcgaagaaga tctccgctcc ctcggagcgc
     3421 ccgatccagt tggtctggag cgccctgacc cgctcgggcc actcgatgtt cgagaagtcg
     3481 aggagctcct ctgcgtagtc cgtgatcttg aagagccact gcaccaggtt cttccttatg
     3541 accggcgtcc cgcaccgctc gcagcgcctg tcgggcccga cgacctgttc tctggcaagc
     3601 gtcgtgttgt ccttcgggca ccagtccaca ggcacctctt tgcggtacgc cagccccttc
     3661 tcgaagaact tgagaaagaa ccactggttc cagcggtagt actcggggtc gcacgtgacg
     3721 acctcggcgt cgaagtcgaa catggtgccc atctgccgga tctggccgcg catgttctcg
     3781 atgttggata tcgtccacct gtaagggtgt atcccgcggt ttatcgcggc gttctccgca
     3841 ggcagcccga acgcgtcgaa gcctatcggg aagaagacct tgtagccgcg catccgcatg
     3901 aagcgcgccc gcgtgtccga aggcgtcatc gcgtaccagt gcccgacgtg caggtccccg
     3961 ctcgggtaag ggagcatcgt gagcgcatag tgcttggtct tctccgggtc ctcgtcggtc
     4021 ctgtacagac ccgactcggc ccagcgctcc tgccagcgcc gttcgagggt tatgggatcg
     4081 taatctcttt tcgtagtaga cgcctcgctc acgagagact ccttcgggta cgggatcggg
     4141 gtagttctag accggtaaag acgcgcagag ttttatagtc gggagtccga cttctgaacc
     4201 ttgtgatccc tggcacgcat atactcaata tatcatcggc ggtactgcct acgaagacca
     4261 acgatccgcc ccggccc
//
LOCUS       DASTUV010000554         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_112121, whole genome shotgun sequence.
ACCESSION   DASTUV010000554 DASTUV010000000
VERSION     DASTUV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112121"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..699
                     /locus_tag="VFT03_11190"
     CDS             274..699
                     /locus_tag="VFT03_11190"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4848755.1"
                     /translation="MGPAVFLAVSAGVAIAFQVVVNAVGLRDLGLGGLISVSGAMTAF
                     AGLAWALFAARPDVTGRALMCALASGLLGAFILSAIVLAANRAGLAQTLSLVIASQLV
                     FGVAIDRLGVFGPTTQELGILKIFGIALILAGGILVVRA"
     gene            complement(800..2050)
                     /locus_tag="VFT03_11195"
     CDS             complement(800..2050)
                     /locus_tag="VFT03_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU4848756.1"
                     /translation="MIEESIVKDPALAPEGRRKIDWVAQHAPVLNAIFEERLSDGSLG
                     GRKIAMTIHLEAKTAYLAILLSEAGAEVTVSGSNPLSTQDDVCAALAERGVRVFATHD
                     PSEDEFEEFLLRTIETGPEIIIDDGAELVSRMILHRPDLLEGVIGASEETTTGILKLR
                     AMDTEKALTFPVLAANDARMKHLFDNRYGTGHSSIVSLLSNTNLFLSGKTVVVMGFGW
                     VGRGLAKYAKGMGARVVVCEPEPVKLLEAYAEGYEAMNSLAAAGIGDVFITGTGNLKV
                     LGSEHFARMKDGVLLANAGHYDHEFDLAALAREAVAVREARRNVTEYEMEDGRRFHVL
                     ARGRLVNIAAADGHPVEIMDLTFAVQALAAHLLANHASEMTPGLHTIPDEIDEYVART
                     KLATLGVEPEELTEEQIEYLTSWR"
     gene            complement(2120..>2716)
                     /locus_tag="VFT03_11200"
     CDS             complement(2120..>2716)
                     /locus_tag="VFT03_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU4848757.1"
                     /translation="VAGMTLEEVRRLDAGNSFSPDGGQTFPYRGRGVRIPALAEVYEE
                     FPNTCVNADIKEARPGAEKAVLRVIQDAAAEGRTLVASTDHAVLRRFRKVSGGRISTG
                     ASRREIAAFYVLSKLHLEALLNPAYEALQAPVEHQGIELVTPRFLKAAHSSGVRVDVW
                     TINDAAEMRRLLDLGVDVIMTDYPEVLESLLQERGGQR"
BASE COUNT          410 a          869 c          909 g          528 t
ORIGIN      
        1 ctcgcgcgag ccttgaggga cgccgggcga aacgagcgcc gtgcagggcg gcgctttgcg
       61 caccagcggg caagctggat catggacctt gtgcggggaa cgcttgcagg taaggggatt
      121 ctcgagcagc cggcctgcga ggagtgcctc tcggaatagt agaaggcata gctggagcta
      181 ccgctggtcg ccaacgggtg catcgccggt agacggcgag acggagcgct ggtagtctgc
      241 ggcgtttcgg ttcgggcgac gagggaggtc cgtgtgggtc cggcggtgtt cttggcggtg
      301 tcggccgggg tggccatcgc gtttcaggtt gtcgtcaacg cggtcgggtt gcgggacctc
      361 ggtctcggcg gcctcatcag cgtctcgggc gccatgacgg ccttcgcagg actggcctgg
      421 gccctcttcg ctgcgagacc cgacgtcacc gggcgcgcgc tgatgtgcgc cttggcgtcc
      481 gggctgctgg gcgcgttcat cctgagcgcc atcgtgctgg cggccaaccg agcggggctc
      541 gcgcagacgc tctcgctcgt catagcctcc cagctcgtct tcggagtggc aatcgaccgc
      601 ctcggcgtat tcggccccac aacgcaagag ttgggaatcc tcaaaatctt cgggatagct
      661 ctgatcctgg ccggcggaat cttggtggtc agggcgtgat caagtttcag gtaatggacc
      721 tcggtagcac atgcgtcgcg tttgtggggg ctttcgaagg cgaactagag cctctctaca
      781 accgctgtag cccgatgccc tatctccagc ttgtcaggta ctctatctgc tcctcggtga
      841 gctcctcggg ctcgacgcca agggttgcga gcttggttcg ggccacgtac tcatctatct
      901 cgtccgggat ggtgtgcaga cccggcgtca tctcagaggc gtggttggct aggaggtgcg
      961 ccgcgagcgc ctggacggcg aaggtgaggt ccatgatctc gaccggatgc ccgtccgcgg
     1021 cggcgatgtt gacgagcctg ccgcgagcga ggacgtggaa gcgccgccca tcctccatct
     1081 cgtactccgt gacgttacgg cgagcttccc ggaccgcgac tgcctccctg gctagcgccg
     1141 cgaggtcgaa ctcgtggtcg tagtgtccgg cgttggcgag gaggacgccg tccttcatgc
     1201 gagcgaagtg ctcgctcccg agcaccttca ggttcccggt tccggtgatg aagacgtccc
     1261 cgatccctgc ggccgcgagc gagttcatcg cctcgtatcc ctcggcgtag gcctcgagca
     1321 gcttgaccgg ctccggctcg cagacgacta cgcgggcgcc catccccttc gcgtacttgg
     1381 cgagcccccg ccccacccag ccgaagccca tcacgactac cgtcttgccg gagaggaaga
     1441 ggttggtgtt ggagagcagg ctgacgatgg atgagtgccc cgtaccgtag cggttgtcga
     1501 agaggtgctt catgcgggcg tcgttggcgg cgagcacggg gaaggtcagc gccttctccg
     1561 tgtccatcgc cctgagcttg aggatgcccg tcgtcgtctc ctcggaggcc cctatgacgc
     1621 cctcgagaag gtcgggtcgg tgcaggatca tgcggcttac gagctcggcg ccgtcgtcta
     1681 ttatgatctc tggtcccgtc tcgatcgtgc ggagcaggaa ttcctcgaac tcatcctcgc
     1741 tcgggtcgtg ggtagcgaag accctgacgc cccgctcggc gagcgcggcg cagacgtcgt
     1801 cctgggtgga gagcgggttg ctccccgaga cggtgacctc tgcacccgcc tcggagagga
     1861 gtatcgcgag gtaggcggtc tttgcctcga ggtggatggt catcgcaatt ttgcgcccgc
     1921 ccaacgagcc gtcggagagc cgctcctcga agatggcgtt caggacaggc gcgtgctggg
     1981 cgacccagtc tatcttgcgt cgtccctcgg gagcgagggc agggtctttt acgatggatt
     2041 cctcgatcat ggctctccta ctctgtctgg cgattggcgc cccaagttta cagggcgcca
     2101 gcgcgggatg ccgctcccgt caccgctgtc ccccgcgctc ctggagcagg ctctcaagca
     2161 cctcagggta atccgtcatt atgacgtcca ccccgagatc caggagccgg cgcatctcgg
     2221 ccgcatcgtt tatcgtccac acgtcaactc gcaccccact ggagtgggcc gccttcagaa
     2281 agcgtggcgt gacgagctct atcccctggt gctctaccgg cgcttgaagc gcctcgtagg
     2341 cggggttgag gagcgcctcg agatgtagct tgctcaacac gtagaacgcg gcgatctcgc
     2401 gcctcgaagc cccagttgag atacgccccc ccgagacctt gcgaaacctc cgcagcacgg
     2461 cgtggtcggt ggaggcgacg agggtccgtc cctcggcggc tgcgtcctgt atgaccctga
     2521 gcaccgcctt ctcggccccc gggcgcgcct cctttatatc ggcgttcacg caggtattag
     2581 ggaactcctc atagacctca gcgagcgcgg gtatcctgac cccccggcca cggtagggga
     2641 aggtctgtcc gccatcgggg ctgaagctat tgccggcgtc gaggcgccgg acctcctcca
     2701 gcgtcatccc tgcgac
//
LOCUS       DASTUV010000555         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1268878, whole genome shotgun sequence.
ACCESSION   DASTUV010000555 DASTUV010000000
VERSION     DASTUV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268878"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFT03_11205"
     CDS             <1..961
                     /locus_tag="VFT03_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU4848758.1"
                     /translation="EESAVMGSGAPRPVKVLYVAGLGRSGSTILANTLGQVEGFFSGG
                     ELNFIWKHALIENRLCGCGKPSQECEFWGPVFETEFGGQSQELAREMMQLQYSGARTR
                     HIPMMLTESGRQRIRARLGKFLDNTGRLYRAIQSVSNSRVVVDTSKEPAYGYAIGMAP
                     GVDLRVLHLIRDPRAAAYSWSKKKRQPDSTEREFMHQTTPAQSAVLWDAWNAAIEALW
                     RQMPARYLRLRYEDFVTDPRRSFQEILELAGEEDAALPLVGERDVRLGISHTVSGNPN
                     RFDTGTVELRQDRAWQKQMKPRDRALVTALTLPLLTRYHYSLR"
     gene            complement(993..1610)
                     /locus_tag="VFT03_11210"
     CDS             complement(993..1610)
                     /locus_tag="VFT03_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEU4848759.1"
                     /translation="MKWLKVYGLPKLPLFLARWAMKLGLPGPTWYKWKAASAGTGVIL
                     DEMIRAADDLGYDGQKVGKLAMSRIGEKQGSDLREQLGVKTMKDAVDVVMLANRFFDI
                     EITLTEQGDGTYVINADRCPWFGGMGNEGVPGWDVKPCAAFSTYEKAMVQAINPNVKL
                     SYTEKRTTGGHTCRGVYQYRDEELGDGPTEGVQPEMVSIGKKPKA"
     gene            1852..2514
                     /locus_tag="VFT03_11215"
     CDS             1852..2514
                     /locus_tag="VFT03_11215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4848760.1"
                     /translation="MLRDIEEHLGRVTEILDEPSLKEVEEDTRRLLEDIRGGDPFSPR
                     WAADSRFARLGYLLCRLISPSVVLETGVAYGVSSVFILKALEVNGRGILHSVDLPPLR
                     SEYERFWGIAVPDALKDRWRLHRGSSARVLPRLLREIRTVDLFVHDSLHTRGNMRREF
                     DMVWPHLRNGAVLLADDVERNRAFSELQRKSPALWRVVQDREESPLHGRAAPVVFGIA
                     IK"
BASE COUNT          477 a          822 c          816 g          487 t
ORIGIN      
        1 agaggaatca gcagtgatgg gcagcggtgc tccaaggccg gtcaaggtgc tctacgtcgc
       61 cggactaggg cggagcggta gcacgatcct ggccaacacc ctgggccagg tcgagggctt
      121 tttctcgggg ggcgagttga actttatctg gaagcacgcc ctgatcgaaa acaggctctg
      181 cggctgtggc aagccgtctc aggaatgcga gttctgggga ccggtcttcg agacagagtt
      241 tggcggccag agccaagagc tggcccgcga gatgatgcaa ctacagtact ccggcgcccg
      301 tacccgccac attcccatga tgttgacgga gagcgggagg cagaggatca gggcccgtct
      361 cggaaagttc ctggacaaca cgggcaggtt gtaccgggcg atccagtcgg tctcgaatag
      421 ccgcgtggtc gtcgacacct ccaaggagcc agcctacggt tacgcgatcg gcatggcgcc
      481 cggcgtggac cttcgggttt tgcacctgat ccgagacccc agggcagcgg cctactcctg
      541 gtcgaagaag aagcgccagc ccgacagcac cgagcgcgag ttcatgcacc agacaacccc
      601 ggcccagagc gccgtgctct gggacgcgtg gaacgccgcc atcgaggccc tctggcgcca
      661 gatgcccgcc agataccttc gcttgcgcta cgaggatttt gtcaccgacc ctcgtcgcag
      721 cttccaggag atcctggagc tcgcgggtga ggaagacgcg gcgttgcccc tggtagggga
      781 gcgcgacgtc aggctaggta taagccacac cgtctccggc aaccccaacc gcttcgacac
      841 gggcaccgtc gagctacgcc aggaccgtgc gtggcagaaa cagatgaaac cccgtgacag
      901 ggctctcgtt acagcgctga ccttaccgct gctgacacgc taccattact ccttgaggta
      961 gccggcgcaa cccaccggcg cctcgaagct tcctacgcct tcggcttctt gccgatgctg
     1021 accatctcgg gctgaacgcc ctctgtgggt ccgtcgccga gctcctcgtc gcggtactgg
     1081 tagacgccgc ggcaggtgtg gccgcctgta gtccgctttt cggtgtagga gagcttgacg
     1141 ttggggttta tggcctggac catcgccttc tcgtaggtag agaaagcggc gcagggcttc
     1201 acgtcccagc ctgggacgcc ttcgttgccc atcccgccga accacgggca gcggtcagcg
     1261 tttatgacgt aggttccgtc gccctgttcg gtcagtgtga tctcgatgtc gaaaaagcgg
     1321 ttggctagca tgacgacatc gacggcgtcc ttcatcgtct tgacgccgag ttgctcgcgc
     1381 agatcgctac cctgcttctc gccaatgcgg ctcatggcga gcttgcctac cttctggccg
     1441 tcgtaaccca ggtcgtcggc ggcccggatc atctcgtcca ggatgacgcc cgtccccgcc
     1501 gacgcggcct tccacttgta ccacgtcggc ccaggcagcc cgagcttcat cgcccatcgc
     1561 gccaggaaca gtgggagctt cggaagaccg tagaccttca gccacttcat aatgggtaaa
     1621 cccttcgttc gcttacgtcg gcgcaagtcc cattatactc tgacctccga gcgtggagta
     1681 ccgaaggagc gcccgtgagc cgtgcgtagg gtcgaaccgc ttataaacct tgctctcctg
     1741 ctcccgcggc gtccccggga gtttcgtgac agggtggcag gttatgctga cctgagcctg
     1801 gaacgaatct ggagaagcgc accggcctac gagaccgcga ggtggcaagg tgtgctcagg
     1861 gacatcgagg agcacctggg ccgcgtaacc gagatcctcg acgagccttc cctcaaagag
     1921 gtcgaagagg acacgcgccg gctcctggag gacatccgtg gtggggatcc gttcagcccg
     1981 cgttgggcag cggactccag gttcgcccgc cttggctacc ttttgtgccg tctgatctcg
     2041 ccttccgtag tgcttgagac cggcgtggcc tacggtgtga gctcggtctt tatcctgaag
     2101 gccctcgagg tgaacggccg cggcatactc cacagcgtag acctgcctcc cctgaggagc
     2161 gaatatgaga ggttctgggg tatagccgta ccagatgcgc tcaaagaccg ctggaggctg
     2221 caccgcgggt ccagcgccag ggttctgccc cggctcctcc gagagatccg cacggtagac
     2281 ctcttcgtac acgacagcct gcacacccgc gggaacatgc gccgcgagtt cgatatggta
     2341 tggccccacc ttcggaacgg agccgtactc ctggccgacg atgtggagag gaaccgcgcg
     2401 ttcagcgaac tacagcggaa aagtccggca ctctggcgcg tggtccagga cagggaggag
     2461 tcgccgctcc acggcagggc cgcgcccgtg gtgttcggga tcgcgatcaa atagcatttc
     2521 agcacgcgct ctgtcgggcg cttatcagca cttcagctag acactgttcg catgcctaag
     2581 tttttgctga aagcaagggc cg
//
LOCUS       DASTUV010000556         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1628207, whole genome shotgun sequence.
ACCESSION   DASTUV010000556 DASTUV010000000
VERSION     DASTUV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628207"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFT03_11220"
     CDS             complement(<1..371)
                     /locus_tag="VFT03_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU4848761.1"
                     /translation="MRVASLLPGATEMVHFVGAGDALVGVTHECDYPAGVERLPKLTS
                     SEIEGHTMTSAEIDAAVEKHMTEAGSLYALDAGLLEDLGPDLILTQGLCEVCAVSTSL
                     VERAVAGMRKEPEILTLNPTS"
     gene            complement(379..1488)
                     /locus_tag="VFT03_11225"
     CDS             complement(379..1488)
                     /locus_tag="VFT03_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4848762.1"
                     /translation="MGGSFRIGRAFGIEIKVHWTFFLLLAFFGFTGYQNSGSLPSALL
                     TVGIIVALFVCVLLHEFGHALMAQRVGIETADITLLPIGGLARLKSLPDRPWDEVKIA
                     IAGPLVNVVLAPIFFGIALLLGGSLTMPPNILEGVQSAGQVFVYLGYINVALVLFNLI
                     PAFPMDGGRVLRGLLATRFGMVRATDIASAVGQFFAAAFFLLGLLGGNLLLALIAVFI
                     FFGATGEAQIVRQREQMRGLLVSDVMGTRPRLETVTPYHTFGQVLDSVIHGYQEDFPV
                     VDENGKLVGMLTRNEIMTAAHSPERYASVRDLMKTDFPTISPDADLFTDGARILQESD
                     LKALPVVRDGELIGMLTMEDIGESALLRDIRKLQY"
     gene            1556..3298
                     /locus_tag="VFT03_11230"
     CDS             1556..3298
                     /locus_tag="VFT03_11230"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HEU4848763.1"
                     /translation="MEERTTSYRLVTDAGGLSGIAETLALAEAIGADIETTDLDPRDG
                     KTRLLQLATPEETFVIDVFAVRDLSPLKGILESGPVKALHNSKFDYQFLLAEHGVSLS
                     PLFDTMLAAQLLDGGEYGASYALEAVAERYLDEAVDKSARREDWSGGLSQRQLEYAAR
                     DAAILLPLRERLAGKLEEERLGYISVIEFGAVAAIAEMELAGVKLDLVRWKELEKTVR
                     ERRDKAALALDAQFPEPEGMLPLEGLGPRLNLNSPKQITDAFLSLGIELPDTKVWTLL
                     KVDHPAARLLLEYRELQKKLGTYLETYPSFVHPKTGRIHANFLQCRVPTGRLACTNPN
                     IQQIPHEDEFRRCFVAEEGYVLVIADYSQIELRILAEVSDDPAFVHAFQNGEDLHRVT
                     AATMYGVPIDDVTKEQRTAAKRINFGLMYGRGARSLSAQLGTDEERGRQLIDEYFANY
                     PKVQRFLQRTANRAVRDRELRTLAGRVRKFGNVPVADDRGAMRREAMNYPIQGAASDI
                     AKLALGYVREELEGLDARLINCIHDEFVVECREEISGEVSERTRAAMMRAGEEILNRV
                     PVDVEVAVSREWRK"
     gene            3357..3773
                     /locus_tag="VFT03_11235"
     CDS             3357..3773
                     /locus_tag="VFT03_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4848764.1"
                     /translation="MAFAAKAHWGYPDHWMERWREDLAITPEYVQQNEVHVAIVEGGP
                     AGFYALAGEGRRIELGHLWVLPERMGEGVGRALFEHALRTAASLGAGVVEIEADPNAE
                     GFYRRMGASRVGEIVYEIDGRERVLPLLAMYVQAPS"
     gene            complement(3944..>4216)
                     /locus_tag="VFT03_11240"
     CDS             complement(3944..>4216)
                     /locus_tag="VFT03_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU4848765.1"
                     /translation="GGLALLDPRLLLAGFGVAMLSSAIPFSLELEALRKLPARVFGVL
                     MSLEPAVAALVGFVVLGERLGTRSLAAIVLVTVAAAGASRFGGKRG"
BASE COUNT          797 a         1322 c         1420 g          677 t
ORIGIN      
        1 aacgaggtcg ggttgagggt gaggatctcg ggttccttcc tcatcccggc taccgccctc
       61 tcgacgaggc tcgtggagac ggcgcagacc tcgcagaggc cctgggtgag gatcaggtcc
      121 ggcccgaggt cctcgagcag cccggcgtcc agagcgtaga ggctccccgc ttcggtcatg
      181 tgtttttcga cggctgcgtc tatctcggcg ctggtcatgg tgtgaccttc gatctcgctg
      241 ctcgtgagct tcgggagcct ctcgacgcct gcggggtagt cgcactcgtg ggtcacgccg
      301 accagagcgt cgcccgcgcc gacgaagtgg accatctcgg tggcgcccgg caggagcgag
      361 gctacgcgca cgaggctcct aatattgcag cttccggata tcgcgcagca gggcggactc
      421 tccgatgtcc tccatggtga gcataccgat gagctcacca tctctgacca cgggcagggc
      481 cttgaggtcg ctctcctgca ggatgcgggc gccgtcggtg aagaggtcgg cgtcgggcga
      541 gatggtcggg aagtccgtct tcatcaggtc gcggacgctc gcgtacctct ccggtgagtg
      601 ggctgcggtc atgatctcgt tgcgcgtgag catacccacc agctttccgt tctcgtccac
      661 tacggggaag tcctcctggt agccgtggat cacggagtcg aggacctgcc cgaaggtgtg
      721 gtagggcgtg acggtctcga ggcgcggcct ggttcccatg acgtcggaga ccaggaggcc
      781 gcgcatctgc tcgcgctgcc tgactatctg cgcctccccg gtcgcgccga agaagatgaa
      841 gacggcgatg agggcgagca gcaggttgcc gccgagcagc cctaggagga agaaggctgc
      901 tgcgaagaac tgcccgaccg ccgacgcgat gtccgtcgcc ctgaccatgc cgaatcgagt
      961 ggcgagcagg ccccgcagca cgcgcccgcc gtccatgggg aaggcgggga tcaggttgaa
     1021 gagtacgagg gcgacgttta tgtagccgag gtagacgaaa acctgccccg cggactgcac
     1081 cccttcgagt atgttagggg gcatcgtgag gcttcctccg agcagcaggg ctatgccgaa
     1141 gaagatcggg gcgagcacga cgttgaccag cggccccgcg atggcgatct tgacctcgtc
     1201 ccacggccta tctgggaggg acttgagccg ggcgagcccg ccgatgggca acagcgttat
     1261 gtccgccgtc tctatcccga cccgctgcgc catgagggcg tggccgaact cgtggagcag
     1321 cacgcagacg aacagcgcga ctatgatgcc gacggtgagc agcgcgctcg gcaaactgcc
     1381 cgagttctgg tagccggtga agccgaagaa ggccagaagc aggaagaagg tccagtggac
     1441 cttgatttct ataccgaacg cgcgacctat cctgaacgag ccgcccatgt cgaggagagt
     1501 ttacctcaaa gcccgagata ccgagcataa tcgtattatc ttgtagaatc ggtccatgga
     1561 ggaacgtacc acgagctacc ggctcgtcac cgacgcggga gggctctcag gcatagcgga
     1621 gaccctcgcg ctcgccgaag ccataggcgc ggacatcgag acgacagacc tcgatcctag
     1681 ggacgggaag acacgccttc tccagctcgc tacccccgag gagactttcg ttatcgacgt
     1741 gttcgcggtg cgtgacctct ccccgctcaa aggaattttg gagagcgggc ccgtaaaggc
     1801 attacacaat tccaagttcg attaccagtt cctgctcgcc gagcacggcg tctcgctctc
     1861 ccccttattc gacactatgc tagccgccca gctcctcgac ggcggggagt acggggcctc
     1921 gtacgcgctc gaagccgtgg cggagcgcta cctggacgag gctgtggaca agtctgcccg
     1981 ccgcgaagac tggtcgggag gactcagcca gagacagctg gaatacgccg ccagggacgc
     2041 agcgatcctg ctgccgctgc gcgagcggct cgccgggaag ctggaggagg aaaggctggg
     2101 ttacatctcg gtgatagagt tcggggcggt cgccgcgata gcggagatgg agctcgccgg
     2161 agtcaagctc gacctcgtgc gatggaagga gctcgagaag accgtacgcg agcggcgaga
     2221 caaggctgct ctcgcgctcg acgcgcagtt ccccgagccg gaggggatgc tgccgctcga
     2281 agggctgggg ccgcggctca acctcaacag cccgaagcag atcaccgacg ccttcctctc
     2341 ccttggcatc gagctgccgg acaccaaggt gtggacgctc ctgaaagtag accatcctgc
     2401 agccaggctc ctgctcgagt accgcgagct gcagaagaag ctcggcacct accttgagac
     2461 ctacccgagc ttcgtccacc cgaagaccgg acgcatccac gccaacttcc tccagtgcag
     2521 ggtacccacc gggagactgg cgtgcacaaa cccgaacatc cagcagatac cgcacgagga
     2581 cgagttcagg cgctgcttcg tcgcagagga gggttacgtc ctcgtaatcg ccgactactc
     2641 gcagatcgag ctgcggatcc tggccgaggt ctcggacgac ccggctttcg tccacgcctt
     2701 ccagaatggc gaggacctgc atcgtgtaac ggctgcgacg atgtacggcg tcccgattga
     2761 cgatgtcacc aaggagcagc gcacggcggc caagcggata aacttcggcc tgatgtacgg
     2821 ccggggggcg agaagtctct cagcccagct cggcacggac gaggagcggg gccgccagct
     2881 catagacgag tacttcgcca actacccgaa ggtgcagcgc ttcctgcaac gcacggccaa
     2941 ccgggccgtg cgggaccgcg agctgcgcac actcgccggc cgtgtcagaa agttcggcaa
     3001 cgtccccgtg gccgacgacc gcggggcgat gcgccgcgag gctatgaact atccgatcca
     3061 gggggcagct tccgacatcg ccaagctggc tctgggctac gtgcgtgagg agctcgaagg
     3121 gctcgatgcc cggctcataa actgcatcca cgacgagttc gtcgtcgagt gccgcgagga
     3181 aatatccggg gaggtttcgg agaggacgag ggcggcgatg atgcgggcag gggaggagat
     3241 cctgaacagg gtgcccgtgg acgtcgaggt ggccgtctcc cgcgagtgga ggaagtgaga
     3301 gcccgaccct ggagatccgg cgcgcacagc ctgacggcgc aaacgtcctg agccggatcg
     3361 ccttcgccgc caaagcgcac tggggctacc ccgaccactg gatggagcgc tggcgggagg
     3421 atctggccat tacccccgaa tatgtccaac agaacgaggt ccacgtcgcg atcgtcgagg
     3481 gaggaccggc tggcttctac gccctcgccg gggaggggcg gaggatcgag ctaggccacc
     3541 tctgggtgct gccggagcgc atgggcgagg gggttggacg cgcgctgttc gagcacgccc
     3601 tgcgcacggc cgcttcgctc ggtgccgggg tagtggagat cgaggcggac cccaacgcgg
     3661 agggcttcta caggcgcatg ggcgcgagtc gcgtcggcga gatcgtctac gagatcgacg
     3721 gccgggagcg cgtgctgccg ttgctcgcga tgtacgtaca ggctccgagc tagctttaca
     3781 ggatacgttc gagggcctcg cagaagcggg agacctcttc ctcggtgttg ttgaagtgca
     3841 gaacttcgtc gcaccccggg gtgtcttgcc aggcgcgcga gaggtccgtc tccgaaaacc
     3901 caccgctcga cttagccccc tctctggcct accacgagct attctagccg cgctttcccc
     3961 cgaaccgtga ggcacctgcg gcggccacag tgaccaggac gatggctgcg agcgagcggg
     4021 tcccgagcct ctccccgagg acgacgaaac ccacgagagc agcgacggcg ggctcgaggc
     4081 tcatgagcac gccgaagacg cgggccggca acttgcgcaa cgcttccagc tcgagcgaga
     4141 acggtatggc cgacgaaagc atcgcgacac cgaagcccgc gaggagcagt cttgggtcga
     4201 gcagcgcgag tccccc
//
LOCUS       DASTUV010000557         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1485764, whole genome shotgun sequence.
ACCESSION   DASTUV010000557 DASTUV010000000
VERSION     DASTUV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485764"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFT03_11245"
     CDS             complement(<1..364)
                     /locus_tag="VFT03_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848766.1"
                     /translation="MTVDGWVPGREPGIEAVFALVNGYLGTRAALEEGSDVSDPATFI
                     NGVFDTVTEEVMQAAATPEHQIIAAPTPELVVAPDWSKLRLTVEGEPLKIDSAELLEQ
                     RRTLDMRRGVLIREWLIRS"
     gene            complement(725..1828)
                     /locus_tag="VFT03_11250"
     CDS             complement(725..1828)
                     /locus_tag="VFT03_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4848767.1"
                     /translation="MKIAEIAPPWVKVPPERYGGIEWIVSLLTDGLVQRGHHVTLYAP
                     GTSTTKGELRAPFDEAVELGGMDFYSAVHPMAVQSVAPFLEAERYDIIHDHTGPIAAC
                     LGALSDTPVLHTLHGPFNPDVKHLFSMIKDKIYFNSISDAQRSGFPELNYIGTIYNAV
                     DLSSYPFRSEKDDYALFLGRFTKDKGAHNAIYIAQKAGVPLRMAGKVDPGEDARYFEE
                     HIEPHLDGEHIVFEGEVGGEQKRKLLSGARFLLFPIQWEEPFGLVMTEALACGTPVVA
                     TAMGSAPEIVIDGEVGILRPPYRWDEMVSAIRGGRLEGIDPHRCREVVRERFSAEAMV
                     DGYEKAFEKILALEKAPGTRRHRLDRTGTLQER"
     gene            complement(1928..2806)
                     /locus_tag="VFT03_11255"
     CDS             complement(1928..2806)
                     /locus_tag="VFT03_11255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4848768.1"
                     /translation="MQRLGFVGLGAMGSPMAGRLAEAGFELKVFDVVAARASPLVEMG
                     AASAASPKKAAEGSGALVLMVANAAQANDVLFGEGGVAGALPEGAAVVVMSTIGPEAV
                     REIDQELAGRGLRTLDAPVSGGVARAERGDLLIMAGGPRDLFEELRPVLEAMGSSVVH
                     CGPRVGDGQAVKLVNQLLCGVHIAAAAEALAYAESLGLDPRFVFETIRHGAANSFMLE
                     DRGERMLEEEYVPPKSALDIFVKDMGLVGNAAEERGFEAPLAAAALEMYLAGRDAGLG
                     AEDDSGVIRVFRRRMT"
     gene            complement(2809..3666)
                     /locus_tag="VFT03_11260"
     CDS             complement(2809..3666)
                     /locus_tag="VFT03_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU4848769.1"
                     /translation="MRVDTMFGAVLARCREAGRAVGAFTCYDLLGFEAVVRAAESRRA
                     PVIVLVSPSSFGAEGGERLVRAFVAASRGAPVEVLVQLDHARDERLIGRAAGCGVDAV
                     MADGSKLPFEENLAFTTTVALSMRSRGVGVEAELGRVEGHEDEADETLSGEMTEPDDA
                     ERFVDETSVDCLAVAVGNVHGHYSGAPDLDWRRLEEIRDRVSAPLSLHGASGLPEEDL
                     RRAVSLGVAKFNVNTELRAAYFGLLEDELVNRTAALDLKSLGDGVVEAVQEVVESKLS
                     AFGWTREEA"
BASE COUNT          638 a         1322 c         1175 g          680 t
ORIGIN      
        1 cgctacgaat gagccactcg cggatcagga cgccacggcg catgtctagc gtccggcgct
       61 gttcgagcaa ctcggctgaa tctatcttga gcggctcacc ctctacggtg agccgcagct
      121 tcgaccagtc tggcgccacg acgagctcgg gcgtgggcgc cgcgattatc tggtgttcgg
      181 gcgtcgcagc ggcctgcatg acctcttcgg taacggtgtc gaagacgccg tttatgaacg
      241 tcgcggggtc ggagacgtcg ctgccctcct cgagggcggc gcgggtgccg aggtagccgt
      301 tgaccagcgc gaagacagct tcgatccccg gctcgcgccc gggcacccag ccgtcgaccg
      361 tcaagatcca gcgccgatcg agatgcttcg ggaacaattc ctgctccgcg cccatcagcg
      421 agacccgagc cggggaccgc tcgggcgcgg cttgccagca aatgccgccg gggtaagacg
      481 cccggctgtg cggctggtcc gaggtcgagt gtcacgccgg tctgtctcca tcaggagcgt
      541 gccgcgtcag gcccgctcat ggggcggaat ctgccagcac gccgtcaacg taagatctag
      601 ccacggtagg caaggctaca gttactcttt cggagttaca agacctgcgg ccgggacgag
      661 ccgccggctc cgtcagggta tcaccccgac tcaccatggt gagtcgcctc gggtagatcg
      721 cggatcaacg ctcctgtaac gttcccgttc tatctagcct gtgtctccgg gttcccggcg
      781 ctttctcgag ggcgaggatc ttctcgaatg ccttttcata gccatccacc atcgcctcgg
      841 cgctgaaacg ctccctcacc acctccctgc accgatgggg atctatccct tccagacgcc
      901 ctccccttat ggccgagacc atctcgtccc accggtaggg tggcctcagg atgcccacct
      961 ccccatctat gactatctct ggagccgagc ccatagcagt tgccaccaca ggagtgccac
     1021 atgccaaggc ttctgtcatc accaacccaa agggctcctc ccactggatg ggaaacaaaa
     1081 ggaagcgtgc tcctgagagc agcttgcgct tttgctcccc tcccacctcg ccctcaaaga
     1141 cgatgtgctc gccatcgaga tggggctcta tgtgctcctc gaagtatctg gcgtcctctc
     1201 ccgggtctac cttgcctgcc atgcgcaggg gcactcctgc cttctgggct atgtagatgg
     1261 cgttgtgggc tcccttgtct ttagtgaacc tgcccagaaa gagcgcatag tcatccttct
     1321 ccgagcggaa ggggtaggag ctgagatcaa cggcgttgta gatggtgcct atgtaattca
     1381 actccgggaa gccggaacgt tgggcgtctg agatggagtt gaagtagatc ttgtctttga
     1441 tcatggagaa gaggtgcttg acgtcagggt tgaaggggcc gtggagggta tgaagcacag
     1501 gtgtatcaga gagtgcccca agacaggcgg cgatgggacc tgtgtgatcg tggatgatgt
     1561 cgtagcgctc tgcctccagg aaaggggcca cgctctgcac ggccatcgga tgcaccgcgc
     1621 tgtagaaatc catgccgccc aactccacgg cctcatcgaa gggggcacgc agctcgccct
     1681 tggtggtgga ggtgcctggg gcgtagaggg tgacgtggtg gcccctctgg acaagcccgt
     1741 cagtaaggag gcttacgatc cactctatcc cgccgtatcg ttcgggtggt accttgaccc
     1801 agggtggggc tatctcagct atcttcaaag ggacctcgct tttctggcga tccgcgccga
     1861 cgccctagct acatctacct gctgcgcgtg ccaccgttta gtgtacacaa caaggcgctc
     1921 ggcctggcta ggtcatccga cgccggaaca cccggatgac ccccgagtcg tcctcggcgc
     1981 caaggccagc gtcgcgcccc gccaggtaca tctcgagcgc cgccgcggcc agaggggcct
     2041 cgaagcctcg ctcctcggcg gcgttgccca ctaggcccat gtccttgacg aagatgtcca
     2101 gtgcgctctt cggcgggacg tattcctcct cgagcatccg ctcgccgcgg tcctcgagca
     2161 tgaaggagtt ggcggcgccg tgccggatcg tctcgaagac gaagcgcggg tccaggccca
     2221 gagactccgc ataggccagt gcctctgccg ccgccgcgat gtgcaccccg cacaggagct
     2281 ggttgacgag cttgacggcc tgcccatcgc ccacccgcgg gccgcagtgc acgacgctcg
     2341 agcccatcgc ttcgaggacc gggcgcagct cctcgaagag gtcccgggga cctccagcca
     2401 ttatcagaag atctcctctc tcagccctcg ccacgccccc gctcacgggc gcgtccaggg
     2461 tccggaggcc acgcccggcc agctcctggt cgatctctcg gaccgcctct ggtccgatgg
     2521 tgctcatgac gaccacggct gccccctccg gcagtgcccc ggcaacgcct ccctcgccga
     2581 agagaacgtc gttcgcctgt gccgcgttcg ccaccatgag gactagcgct ccgctgcctt
     2641 ccgccgcctt cttcggggag gcggcgctcg cggcccccat ctcgacgagg ggactggcgc
     2701 gcgcggctac gacgtcgaag acctttagct cgaagcccgc ttcggccagc ctgccggcca
     2761 tgggggaacc catcgctcca agcccgacaa accccaaacg ttgcactgct acgcctcctc
     2821 tctcgtccag ccgaaggctg agagtttcga ctccacgacc tcttgcaccg cctcaactac
     2881 tccgtcgccc agagacttga ggtcgagcgc cgctgtcctg ttgacgagct cgtcttcgag
     2941 cagcccgaaa tatgccgccc tgagctcggt gttgacgttg aacttggcta cccccaggga
     3001 gacagcccgc cgcaggtctt cctcggggag ccccgaagcg ccgtgcaggg agaggggagc
     3061 ggagacgcgg tcccggatct cctcgagcct ccgccagtcg agatccgggg cgccggagta
     3121 gtggccgtgg acgttgccga cagcgaccgc gagacagtcc acgctggtct cgtccacgaa
     3181 tcgctctgcg tcgtccggct ccgtcatctc gccgctcaaa gtctcgtctg cctcgtcctc
     3241 gtggccttcg acccggccga gctcggcctc gaccccgacg ccgcgggaac gcatggagag
     3301 cgccacggtc gtggtgaagg ccaggttctc ctcgaagggg agcttcgagc cgtcggccat
     3361 cacggcgtcc acaccgcagc cggcggcgcg tcctatgagg cgctcgtccc tggcgtggtc
     3421 gagctgcacg aggacctcga caggagcgcc cctggacgcg gctacgaacg cccgcacgag
     3481 gcgctcgccg ccctcagctc cgaaagacga agggctcacc aggacgatca cgggcgcccg
     3541 gcgcgactcg gccgcccgga ccacggcttc gaagccgagg aggtcgtagc aggtgaaggc
     3601 gccgactgcc cgccccgctt cccgacaccg ggccagaacc gccccgaaca tcgtgtcgac
     3661 ccgcattccg cccccttagc gccgtctccg tcgagcgttt ctaccgctct ccgcgcagga
     3721 cttctatggc ccgggccaga gaatccgggc cgccgacgtt cccggggaag atcacgtacg
     3781 gcatccccgg gaagtcgttg tcgccgggga ggatc
//
LOCUS       DASTUV010000558         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_1178683, whole genome shotgun sequence.
ACCESSION   DASTUV010000558 DASTUV010000000
VERSION     DASTUV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178683"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFT03_11265"
     CDS             <1..723
                     /locus_tag="VFT03_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4848770.1"
                     /translation="YGDSELHYGRWMPEIRDQIFLSTKTGERNGDAARRQIEASLERL
                     QVDRVDLIQLHAVGDLEDLDRATSSGGSLESAVEAREEGLVGAVGITGHGYEAPATHL
                     EALRRYPFDTVLTPWNYILSTDESYSRDFEALVEEIVRQDAGLMTIKTVSRRNWPEGN
                     PLEGQRRATWYEPFEEQEYVDAAVSFVLARGEITGIPMVGDASLIETMIEAERRRIPL
                     EEAEQTLARAPDYSSPFISIPF"
     gene            777..1739
                     /locus_tag="VFT03_11270"
     CDS             777..1739
                     /locus_tag="VFT03_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4848771.1"
                     /translation="MQTVRFGKTGMEITPIGFGAWAIGGGGWAAAWGPQDDDEAVGAI
                     RRAVELGVNWVDTAAVYGLGHSEELVAQALKSVPESERPYVFTKCSLVWDDAGDISNV
                     LEKNSVKRECEESLRRLQTDVIDLYQIHWPRPDEYIEEGWEAMAELKDEGKVRHIGVS
                     NFDVSQMERIAEIAPVETLQPPYNMLNRGVEEEILPYCGENDTGVIVYSPMRSGLLTG
                     KMTPERVANLPSDDWRRNAQDFQEPRLSRNLELVELLEEIGADHGFSPGEVAIAWTLR
                     QPAVTAAIVGGRRPDQVDGIIGAAELRLSEDELDRIESFLTQNP"
     gene            1825..3441
                     /locus_tag="VFT03_11275"
     CDS             1825..3441
                     /locus_tag="VFT03_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU4848772.1"
                     /translation="MFSVALLQSEVQVNDTWLDYFIIAVYFVFVLGIGYVLRTKMQTS
                     EDYFLSGRSLPSWITGLAFLGANLGALEIMGMAAGAAEYGIMQSHYYWIGAIPAMVFV
                     ALFMIPFYYASRVHSVPGFLRLRYNEATRGFNSILFAIFMILLSGINMFAMALVFQLI
                     LGWSLTASIFLSAGIVLAYVTLGGLSSSIYNEVLQFFLITLGLVPLVVFGLIDVGGLS
                     GLRQSIGKPEFFHVWANTGSTDNPFAVQWFYIVFGLGLVQSFGYWCTDFLVVQRALAA
                     EDLAASQRTPLIAAFPKLMYGVLAIFPGLIALSILPKVTANDPNNAVPFAMAHYFPTG
                     MLGFGLTALLAAFMSGMAGNVTAFNTVWTYDIYKPYINRDAPDRHYLNVGRIATVVGV
                     LLSIATAYLALGFTTIMDYVQVLHGLFLAPLFGTFLLGMFWRRTTGWGGFTGLVAGTA
                     TGLTMYLISLDAGLGASMARAFWAWIVTLVVTIAVSLVTQPKPDSELRGLVWGLTEQK
                     EGVEEIWYKKPWVLAVTVLTITVALNIYFW"
     gene            3451..>3641
                     /locus_tag="VFT03_11280"
     CDS             3451..>3641
                     /locus_tag="VFT03_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4848773.1"
                     /translation="MTEERQTEHHDYLEEERVPEHSGTNHSIAIGFYISAIVLIYGVT
                     IVLAGLLSTGSQTEGNLGF"
BASE COUNT          675 a         1124 c         1136 g          706 t
ORIGIN      
        1 tacggcgact ccgagctgca ctacggtcgc tggatgccgg agatccggga ccagatcttc
       61 ctctccacca agacgggcga gcgcaacggt gacgctgcca ggcgccagat cgaggcgtct
      121 ctagagcgcc tgcaggttga ccgcgtggac ctgatccagc ttcacgccgt cggcgacctc
      181 gaggacctcg accgcgccac cagctccggc ggctcgctcg agtccgccgt cgaggcgcgt
      241 gaggagggcc tcgtcggcgc agtgggaata accggccacg gctacgaagc cccggcgacg
      301 cacctcgaag ccctgcgccg ctacccgttc gacacggtac ttacgccctg gaactacatc
      361 ctctccacgg acgagagcta cagccgcgac ttcgaagcgc tcgtcgagga gatagtgcgc
      421 caggacgcgg gcctcatgac tataaagacc gtctcgcggc gtaactggcc cgaggggaac
      481 ccactggaag gccagcggcg ggcgacctgg tacgagccct tcgaggagca ggagtacgtg
      541 gacgcggcgg tctcgttcgt gctggcacgc ggggagatca cgggtatacc gatggtcggg
      601 gacgccagtc tgatagagac catgatcgag gccgagaggc gcaggatacc gctcgaagag
      661 gccgaacaaa ccctcgccag ggcccccgac tactcctcgc cgtttatctc gatacctttc
      721 taggcttcag ggaaaatccg tagcctgaca cctgagacct acaaaaggag gctgctttgc
      781 agacggttag gttcggcaag acggggatgg agatcacacc tataggcttc ggggcctggg
      841 cgataggcgg gggcggctgg gccgccgcct ggggaccgca ggacgacgac gaggcggtcg
      901 gcgccataag gcgggctgtc gagctggggg ttaactgggt ggataccgcc gccgtctacg
      961 gtctcggcca ctccgaggag ctcgtagcac aggctctgaa gagcgtaccg gaatccgagc
     1021 ggccctacgt cttcaccaag tgctcgctcg tgtgggacga cgcgggcgac atctcgaacg
     1081 tcctcgagaa gaactcggta aagcgcgagt gcgaggagag cttgcggagg ctccagaccg
     1141 acgtcataga cctctaccag atccactggc cccgccccga cgagtacatc gaggagggct
     1201 gggaggccat ggccgagctc aaggacgagg gcaaggtccg ccacatcggg gtctcgaact
     1261 tcgacgtctc ccagatggag cggatagcgg agatagcgcc cgtcgagacc ctgcaaccgc
     1321 cctacaacat gctcaaccgc ggcgtcgagg aggagatact cccgtactgc ggggagaacg
     1381 acaccggggt aatagtctac tcgccgatga ggagcggtct gctcacgggc aagatgaccc
     1441 ccgagcgcgt ggccaacctc ccttccgacg actggcgccg caacgcccag gacttccagg
     1501 agcccaggct ctccagaaac ctcgagctcg tcgagctgct ggaagagata ggcgcagatc
     1561 acggcttttc gccgggtgag gtcgccatag cctggacgct caggcagcct gcggtgacgg
     1621 cggccatcgt gggtggacgg aggccggatc aggtagacgg catcatcggt gctgcggaat
     1681 tgcgtctctc cgaggacgaa ctggacagaa tagagtcctt cctcacccag aacccctagg
     1741 tcgtttgtag cttacttgat ttgttaactc gaagactggg tattgctatg ctgcttacgt
     1801 aggggaaaaa gtaaggggga cgccatgttc agtgtggctt tgctgcagtc ggaggtccag
     1861 gtcaatgaca cgtggctcga ctatttcatc atcgcggtgt acttcgtgtt cgtgctcggc
     1921 atcgggtacg tgctcaggac caagatgcag acctccgagg actacttcct ctccgggagg
     1981 tcgctgccga gctggatcac gggcctagcc ttccttggtg cgaacctggg agcgctggag
     2041 atcatgggga tggccgcagg tgcggcggag tacgggatca tgcagtccca ctattactgg
     2101 atcggcgcga tcccggcgat ggtcttcgtg gccctgttca tgatcccctt ctactacgcc
     2161 agccgcgtcc actccgtccc tggcttcctg aggctccgct acaacgaggc gacacgcggg
     2221 ttcaactcca tcctgttcgc catcttcatg atactgctct cgggcataaa catgttcgcg
     2281 atggcgctcg tcttccagct gatcctcggc tggtcgctca cggcgagcat cttcctctcg
     2341 gcggggatag tactcgcgta cgtgaccttg ggcggtctct cctcatccat ctacaacgag
     2401 gtcttgcagt tcttcctgat cacgttaggt ctggtgccgc tcgtggtctt cggccttatc
     2461 gacgttggcg gcctctccgg tttgcggcag tccataggca agccggagtt tttccacgtc
     2521 tgggccaaca caggctcgac ggacaaccct tttgctgtgc agtggttcta catcgtcttc
     2581 ggactcggtc tggtgcagag cttcggctac tggtgcacgg acttcctcgt ggtgcagagg
     2641 gctctggcgg ccgaggatct ggcggcatcc cagaggaccc cgctcatcgc ggcgttcccg
     2701 aagcttatgt acggggtact cgctatcttc cccggactga tcgccttgtc catcctcccg
     2761 aaagtcaccg cgaatgaccc caacaatgct gttcccttcg cgatggccca ctacttcccc
     2821 accgggatgc tcgggtttgg cctgacggcg ctgctcgcgg ccttcatgag cgggatggcc
     2881 ggcaacgtga cggccttcaa taccgtctgg acctacgaca tctacaaacc gtacatcaac
     2941 cgggacgccc ccgaccgcca ttatctcaat gtgggtcgca tcgccactgt tgttggggtg
     3001 ctcctgagca tcgcaacggc ctacctggcc ctcggattta ctaccatcat ggactacgtc
     3061 caggtgctgc atggcctgtt cctggccccg ttgttcggga cgttcctcct tggcatgttc
     3121 tggaggcgca cgacgggctg gggtgggttc accgggctcg ttgctggaac ggctactggc
     3181 cttaccatgt acctcatctc gctggacgcc ggcctggggg cgtccatggc gcgagcattc
     3241 tgggcctgga tcgtgaccct cgtcgtaacg atcgcggtct cactggtcac ccaacccaag
     3301 ccggactctg agctgcgcgg cctcgtgtgg ggcctgaccg agcagaagga gggcgtggag
     3361 gagatctggt acaagaaacc gtgggtcctc gcggtgaccg tgctcactat taccgttgcc
     3421 ctgaacatct atttctggta ggaggagacc atgactgaag aacgccagac agaacaccat
     3481 gattacctgg aagaagagag ggtgccggag cactccggca cgaaccactc tatcgcgata
     3541 ggcttctata tctcggccat cgtcctgatc tacggcgtca cgattgtcct ggccgggctg
     3601 ctctctaccg gatcacagac cgagggcaac ctcggcttta a
//
LOCUS       DASTUV010000559         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U2342
            k141_89464, whole genome shotgun sequence.
ACCESSION   DASTUV010000559 DASTUV010000000
VERSION     DASTUV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960805
            Sequence Read Archive: SRR25158487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:25:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,226
            CDSs (with protein)               :: 2,226
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89464"
                     /isolate="SMAG_U2342"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.96 N 113.0766667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFT03_11285"
     CDS             complement(<1..831)
                     /locus_tag="VFT03_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU4848774.1"
                     /translation="MLALLQAEVVPRIPLAEWTESGVEWITDTFDGAFDLLDSVIDIV
                     IGTLEFVLLSPPALVMVAILGVIALLLAGWRVALFTVLGLLFVISLDLWEAAMQTLAL
                     VLAATVVALAIGIPIGIIAAKSNAVEATIRPVLDIAQTMPAFVYLVPIVVLLGIGAGP
                     ALIATVIFAMPPAVRLTMLGIQQVPSDTVEAAHAFGATPWQTLFKVELPLSLKTIMAG
                     VNQVIMLSLSMVVIAGLAGAGGLGEPVVTGLSQLNVGISFVGGVGIVVIAIMLDRVTR
                     A"
     gene            complement(835..2067)
                     /locus_tag="VFT03_11290"
     CDS             complement(835..2067)
                     /locus_tag="VFT03_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564534.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU4848775.1"
                     /translation="MVFGRRTQQALEMRRAGKSKPEVERATGATIGAYDVSFEVARGE
                     IFVIIGLSGSGKSTILRAVNGLNELTEGKVYLDGDLISEMPPKQLRKVRRKKIGMVFQ
                     HFGLLPNRTVLDNIAFGLEIQGVSAQERRRKAEETLPEVGLEGQGSKLITELSGGMKQ
                     RVGLARALATEQEILLMDEPFSALDALIRRDMQNLFLDIQGEARRTVVFVTHDLDEAL
                     TLGHRVAIMKDGDIVQIGTPEQILTEPANEYVERFVEGVDYAKVRHAESVMVDPKEVA
                     YASEGPTVVLRRMKQAGLSHIFVLDEERRLVGVAEAEEIAKLVERNERELHSAVEDAP
                     SVGRRTPLREVLPLFVEDGSAPVAVVGEGGRLEGVIVRGALIAGLTTSVEPHGEEGLG
                     GVGASENGDRVHVRRVGE"
     gene            2439..2511
                     /locus_tag="VFT03_11295"
     tRNA            2439..2511
                     /locus_tag="VFT03_11295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2472..2474,aa:Arg,seq:cct)
BASE COUNT          488 a          905 c          763 g          464 t
ORIGIN      
        1 tgcgcgagta acgcggtcca gcatgatggc gataaccacg atgcctaccc cgccgacgaa
       61 gctgatcccg acgttgagct ggctcaagcc cgtcactacc ggctcgccga gaccacccgc
      121 cccggccaga ccggcgatga ccaccatcga gagcgagagc atgatgacct ggttgacccc
      181 ggccataata gtcttgagcg agagcggaag ctcgaccttg aagagcgtct gccagggggt
      241 cgccccgaag gcgtgcgccg cctcgacggt gtcgctgggc acctgctgga tgccgagcat
      301 ggtgagcctg acggcgggcg gcatcgcgaa gatgacggtc gcgatcaggg caggcccggc
      361 gccgatcccc agaaggacga cgatgggcac gaggtatacg aaggccggca tcgtctgggc
      421 gatgtcgagt acgggccgga tggtggcctc gaccgcgttg gatttggccg cgattatgcc
      481 tatcgggatg cctatcgcca gcgcgacgac cgtcgccgcc aggaccagcg ccagggtctg
      541 catcgcagcc tcccagagat ccaggctgat cacgaacagc aaccccagca cggtgaagag
      601 cgcaaccctc cagccagcca gcagaagcgc gatcacgccc aggatggcga ccatgaccag
      661 cgcgggcggc gaaaggagga caaactccag cgtaccgatg acgatatcta ttacggagtc
      721 caggagatcg aaggccccgt cgaaggtgtc ggtgatccat tcgacgccgc tctcggtcca
      781 ctcggcgagc ggtatcctgg ggacgacctc ggcttgcaag agagcgagca tctcctactc
      841 ccccacccgg cgcacgtgca cccgatcgcc gttctcgctg gcacccacac cgccgaggcc
      901 ctcttcaccg tgcggctcga cgctcgtggt cagcccggcg atgagcgcgc cgcggacgat
      961 gacgccctcg agacggccac cttcgccgac gacggccacc ggcgcgctcc cgtcctccac
     1021 gaagagcggc aggacctcgc gcagcggggt tctccgcccg acgctcgggg cgtcctccac
     1081 cgcggagtgc agctcgcgct cgttgcgctc gaccaacttg gcgatctcct ccgcctcggc
     1141 gacgccgacc aggcgccgct cttcgtccag cacgaagatg tgcgagaggc cggcctgctt
     1201 catgcgccgc aggaccacgg tagggccctc gctggcgtac gcgacctcct tgggatcgac
     1261 cattacgctc tcggcgtggc gcaccttggc gtagtccacg ccctcgacaa agcgctccac
     1321 gtactcgttg gcgggctccg tcaggatctg ctccggggtg ccgatctgga cgatgtcccc
     1381 gtccttcatg atcgccaccc ggtgccccag cgtgagcgcc tcatccaggt catgggtgac
     1441 gaagacgacc gtccgcctcg cctcgccctg gatgtccaaa aagaggttct gcatgtctcg
     1501 ccgaatgagc gcgtcaaggg cgctgaaggg ctcgtccatc agaagtatct cctgctcggt
     1561 cgcgagcgcc cgcgcgagcc cgaccctctg cttcataccg ccggagagct cggttatgag
     1621 cttgctgccc tgcccctcta ggccaacctc gggcagggtc tcctccgcct tcctcctgcg
     1681 ctcctgcgcg gagacgccct ggatctcgag cccgaaggcg atgttgtcca gcacggtccg
     1741 gttcggcaag agcccaaagt gctggaagac catgccgatc ttcttgcgcc ggaccttcct
     1801 gagctgcttg ggaggcatct cggagataag gtccccatca agatagacct taccctcggt
     1861 cagctcgttt aggccgttga cggcccgcaa gatcgtggac ttcccgctcc ccgagaggcc
     1921 gatgatcacg aagatctcac ccctcgcgac ttcgaagctg acgtcgtagg ctccgatagt
     1981 ggcccccgtc gcccgctcga cctcgggctt gctcttcccc gcccggcgca tctccagagc
     2041 ctgctgcgtc cggcgcccga agaccaccga acacccctcc acccgtatta gagcctcccg
     2101 gctcctgctg tttgcttccg tggcttcccc ttctatagcc tgcaacttat gattgcgcgc
     2161 tctataactc gcactattcc cgtcggttac aacaacaatg cttacaaccg ctcttccatc
     2221 cggttgcgat tctgaatgat aagcggttct tatcacctat tcaactgaaa gagccgttaa
     2281 gttatgttac tttccgcata tctggtatcg tgcaacactt ccataacgcc tgcaaacgac
     2341 gctacgcatg ggcctccgtt catcgcagcc acgtacacgt agcggctcca cgagacgctg
     2401 gtaggagcgg atttcgtggg ttaaactaac cgcgctttgc gcctgtagct caggggatag
     2461 agcagcggtt tcctaaaccg cgagtcggag gttcgaatcc ttccaggcgc actcctgaaa
     2521 gacctgcaaa tgagcgaaaa agaagagagc cccggtttag cgccggggca ctactacacc
     2581 aactactaca ccaacgcaac tacccgagcg cggcgtccat
//
